LOCUS       DAOSOF010000001        49997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_744_length_49997_cov_5.806355, whole genome shotgun sequence.
ACCESSION   DAOSOF010000001 DAOSOF010000000
VERSION     DAOSOF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 49997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_49997_cov_5.806355"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLM68_00005"
     CDS             <1..901
                     /locus_tag="PLM68_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT53785.1"
                     /translation="VVSVAGCVLLEHLIVRYLMRKKTTVHDLSAVVTGLILAVNLPVS
                     SPWWMVLIGCIAAIGIAKMTFGGLGHNLFNPALVGRVFLLISFPVQMTNWSVNNSIFS
                     SETWNLPKILLGAQGQLPDAVSGATPLGIIKEGLANGLTMETLSQQYDFSYWQMLFGH
                     IDGSLGEASALAVLVGFAYLLFRKVIKPLIPVSILFTVAVFSAILWLLDPSMNADPLF
                     HLLTGGLLLGAVFMATDYTTSPMSPPGMLIYGVGIGVMTMIIRVWGVYPEGVSFAILF
                     MNALTPLLNKIKPIPYKKEVSRG"
     gene            921..1484
                     /locus_tag="PLM68_00010"
     CDS             921..1484
                     /locus_tag="PLM68_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOT53786.1"
                     /translation="MFLCLLIICFGGSALLGGVYLITYEPIEAAQVAKVNQALGGVLP
                     QFDNLPAQEAYEVEVGGRKATVYPGTKNGELTGVAVEATTTKGFGGQIKVMVGFLPDG
                     TINNTAIISHTETPGLGDKINPKKSKFPLQFNQKNPESFRLAVKKDGGDVDAITAATI
                     SSRAFCDVVDLAFQVFRKSIQQEGGDQ"
     gene            1481..2062
                     /locus_tag="PLM68_00015"
     CDS             1481..2062
                     /locus_tag="PLM68_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOT53787.1"
                     /translation="MSRMKTLVNGIIKENPTFVLVLGMCPTLGTTTSAINGMSMGLAT
                     TFVLMLSNMVISLVSGLVPSKVRIPAFVVVIAVFVTVVQLVMQAYVPALYETLGLFIP
                     LIVVNCIVLGRAEAYASKNTPFDSFLDGLGSGLGFTLSLTVLGTIRELLGSGSFFGFK
                     LLEGDGILVFILAPGAFMALGYLMVLFNKLKKA"
     gene            2067..2666
                     /locus_tag="PLM68_00020"
     CDS             2067..2666
                     /locus_tag="PLM68_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HOT53788.1"
                     /translation="MEYIIIIISAVFVNNIVLNQFLGICPFLGVSNRISTATGMAAAV
                     VFVMTLSTLFTYLVQYYILDPLNISFMQTVTYILVIAALVQMVEIILKKISPALYQAL
                     GIFLPLITTNCAVLGVAILVVTKEFSFGGTPHMLDLPQSLVYTASMALGFGLALIIFA
                     GMREQMDLVGVPKPFRGVPISLITAGILSLAFMGFSGIV"
     gene            2678..2905
                     /locus_tag="PLM68_00025"
     CDS             2678..2905
                     /locus_tag="PLM68_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT53789.1"
                     /translation="METIEELEKYVKTTEGSRDQNAWYKLGSLYRQQENWGKALNAYA
                     RCMELGASNAAREATEAIKRILDYRNEDWMP"
     gene            complement(2878..3783)
                     /locus_tag="PLM68_00030"
     CDS             complement(2878..3783)
                     /locus_tag="PLM68_00030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT53790.1"
                     /translation="MKFYLSALLSVLLLVFTACEKQPLPNPEPDPDKPVSLGLSCQGA
                     IVIDAASGEALYSLSPDFPLVPASMTKVMTMYIVFDHIEKGYLSLDSMIPISVNAAAR
                     SVNPGESNVPLNQNLTYSVHEMIGALCVVSANACAVALGEYLSGGTESDFSVIMNRYA
                     DSLKLEARYYDAAGMSSQNGITPRSMALLTREFIRRFPVILQYTSLTSLDFRGVTYGA
                     TNNMLPGKTYYYNGCDGFKTGYTSAAGLCITATATLADKRVIAVVMKAPSSFARAQDA
                     TRLMDYGFTVLMNRVSVYGIQSSFL"
     gene            3822..6107
                     /locus_tag="PLM68_00035"
     CDS             3822..6107
                     /locus_tag="PLM68_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT53791.1"
                     /translation="MKKLTWIALLTAISCSSPDYSVVQDLNTGWEFWNDQEPEAIQDV
                     FLPHTPRLEPYVVNDQWQGICWYRKVVDIPPGRHVLVRFEGAMNVAELWVNGHYAGRH
                     MGGYLPFVCDITRVMKENGVFSGQAELKLRLDNHDNPITGPKPLELLDFNMYGGLYRD
                     ARLIIKSPLHITDPILENVPAGGGILVTFPAVSQDSALVEVRTHIRFSPLNDDQAEAQ
                     TFLLEQALLAEDSLCAFSRQEIRLESGQACTALDLLRVEQPALWSPEHPYCYNLETRI
                     IMTGPGGRTVVVDRQRTTVGIREVVFQGKELFVNGSKTFLRGVNRHQEYPYVGYAVPR
                     NAQWRDALKIKEAGFNLVRLSHYPQSPDFMEACNRLGLFVLDAIPGWQYFSDDPAFQE
                     QVIGTARDMIRRDRNHPCVLAWEVSLNESWMSEDFIDRILEASRHEMPVPIHTAGWQE
                     YEYDIFLQARQHRLGHPPVKTPKPLIVSEYGDWEYYALNAGFAQQNWENLQPAERSSR
                     QLREHGQKRLLQQVTNIREAHSSNYHVDACADNYWIMFDYNRGYADDLEASGLMSLER
                     LPKFSWHYFRSLRSPLEHPMVFIADYWEGTDTANVEVQVLSNAEQVALWLNGNLLERR
                     DPPFTFVVPRFEKGWLKAEAYCADKVCARDSVHTPGEAVAVEWWLDTGNIPPAPNDLL
                     FVYAKAVDRQGYPVPSAKGRVLFTLDGKEMCLIGEEPDGSVSRTLRTEAPLRAGNAFA
                     LLQLSPLWDGSEPHITVTLLP"
     gene            6104..6628
                     /locus_tag="PLM68_00040"
     CDS             6104..6628
                     /locus_tag="PLM68_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53792.1"
                     /translation="MKQKIRILIPVLAAAILAVSCQGEEGYTASYYFGSRANMAFSKV
                     TSTLTPGLRELAEMLTQKRDEIQKDYQFDWTVTSSGSTRETAVQNAIGKAIASFTSSM
                     TINFDLDMQSFADEFSAKKKELSHALSGETGSVSFTVHLYLTAEEIIAEAYEMPLAVS
                     KTYTFECRGKNESY"
     gene            complement(6625..7293)
                     /locus_tag="PLM68_00045"
     CDS             complement(6625..7293)
                     /locus_tag="PLM68_00045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT53793.1"
                     /translation="MKKSTLIILIGMSALFMGSCNNTGKKAKEAAAVTANEISTEAAT
                     EQAFDPATLPEEPVFEIKTTEGSMIIRLYSETPQHRDNFVKLAAGHYYDNILFHRVIN
                     GFMIQCGDPESRTATPEQRLGSGGPGYTVPAEFVTGLTHKKGAIAAARTGDAVNPEKA
                     SSGSQFYIVHNPETCRQLDGNYTVFGEVTEGLEIIDRIASVPTGTADRPVTDVRIISV
                     LPVL"
     gene            7363..7797
                     /gene="dut"
                     /locus_tag="PLM68_00050"
     CDS             7363..7797
                     /gene="dut"
                     /locus_tag="PLM68_00050"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT53794.1"
                     /translation="MKIKIINHSPYPLPEYATPLSAGMDLRAHIPGVITLQPLERSLV
                     PTGLFIELPAGYEAQIRPRSGMAARKGIGILNSPGTIDADYRGEIGIILVNFSGQAAE
                     IAPGERIAQLVVASHQRVVWEPVEQLEETERGKGGFGSTGHQ"
     gene            7808..9094
                     /gene="murF"
                     /locus_tag="PLM68_00055"
     CDS             7808..9094
                     /gene="murF"
                     /locus_tag="PLM68_00055"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT53795.1"
                     /translation="MNRLVALFEKHPDICTDTRQIRPGCLFFALKGEHFDGNMFASQA
                     LENGAAYAVVDQYHGTDPRCILVPDVLQALQQLAAVHRSRFAGPVLALTGSNGKTTTK
                     ELIHAVLSVRYNVTATQGNLNNHIGVPLSLLKMGPATEAGLFEMGASHPGEIAFLTDM
                     VKPNAGLITNISGAHLEGFGNLDGVKQCKGALYDYLHAHEGTIFHNADDPELTEMLEQ
                     RTIRDHLIPYGISKQPVQTSLEDGVLVITVSGYPQIRTHLAGLYNTPNVLAALAVARY
                     YGIENTKAAEAVAGYIPSNNRSQWINTGRNALIADAYNANPVSMSLALKNLDSIREGK
                     KCAILADMLELGAYAHQFHLGIVKQVEQMGLEQVFWVGPLFSKAVQELGAAGMCFSKV
                     EDLILHLATRPLEGATVLVKGSNGMGLSKFIASGVC"
     gene            complement(9072..10091)
                     /locus_tag="PLM68_00060"
     CDS             complement(9072..10091)
                     /locus_tag="PLM68_00060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT53796.1"
                     /translation="MMPKVAIVILNWNGKYFLERFLQLLVTRTPEPWARIYVADNGST
                     DGSVAWLRDNFPGMHLISFDRNLGYTGGYNKALEQIEAEYYILLNSDVEVTHGWLQPL
                     VTYMDSQTGCGVCMPKILSWTNPDRFEYAGACGGFIDRLGFPFCRGRILTSIETDHGQ
                     YDKPADIFWASGTCLVVRSVLFHSLKGLDDHFFAHMEEIDFCWRAQWAGYTIAVVPES
                     KILHVGGGTLPNDSPHKLYLNYRNNLLMMYKNLDNGEVVPIIALRLFVDHLSAIAYLF
                     QGKWSFTGSVWKAHRHFWAIRRSVERTKKESARNFSGVVYRGSIVLRFFLSLKKVTFS
                     KLRMQ"
     gene            10127..11956
                     /locus_tag="PLM68_00065"
     CDS             10127..11956
                     /locus_tag="PLM68_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOT53797.1"
                     /translation="MKPKVELLAPARNLECAVEAVNHGADAVYMGADRFGARAAAGNS
                     LSEIGQAVLYAHRYGARIYITMNTLLFDSELEEAVRMARGLYDAGADALIIQDISLLY
                     LRRHNVFPPLPIHASTQMDNASAEKVLYLEKEGFDRVVLARELAPEQIRRIRERTSVE
                     LECFVHGALCVSYSGKCYISQEDCGRSANRGECAQYCRLSYDLEDAAGNVLLRNKHLL
                     SLKDMDRSERLQELIDAGVHSFKIEGRLKDVSYVKNITAYYRQQLDRLQGQKTSLGTC
                     TFFFRPDPQKTFHRDQTGYFPENGRFTEPVHQWYTPKSTGEYLGKINRVFPGGFETNA
                     VVNTGDGLCFTLPDGTFSGFSVNRVEGKRIFREGTPLEKGMEIYRNHDRLFEKQLRGK
                     TAERRMDMDIEIAARNGGLFLSLIRESLPGEPPARVTVPIDCPYDEAKDPSQARDFLS
                     ATVSRMGDTPYRIRKVFFSPVDFPWFVPASVLALAKKEALERMELLVQSNARGFHVTP
                     HELPEQDPLTALQKNYPPGSYLTAATPIGEHTPLMTCRYCLKQALGYCPREKKNTPGT
                     LREPLFLVRGQKRFPVSFDCQECLMLVGRAASSKRARPQEMKK"
     gene            complement(11884..13371)
                     /locus_tag="PLM68_00070"
     CDS             complement(11884..13371)
                     /locus_tag="PLM68_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HOT53798.1"
                     /translation="MKNLLKRILLAALLFVMAGSSALYGQRSLKTIEDLDNPDIRIGV
                     LIATWQESYARERFPKARILPLANTTDLLSLLRSGGCDVVFLAQPPCIYILEEAPDLA
                     FLDERVAPTAVSAGFPLNDTVFLPRFNEFLQELQDNGIHRQMEERWLAESGNTMPDFP
                     ESTTSQKLLRIGTTCMDVPFSFYQNNQPAGFDIELIRRFAAWMGRKPVFVISDFSGML
                     AGMLAGKMDIMVNYIMVTPERTLKYSFSETYLTTYSSMITLRSRLEQGMAVDKPRQGL
                     KERFYNNIILEKRYILLWVGLKITLLIAFLSTLGGTLTGAVICYLRMSRRKILRLMAS
                     GYISFFRGIPQVVLLMLMYYVVFASTKMTGVTVSVITFSMIFGAYVSEMFRSAIQSVP
                     RGQTEAGIALGFSKINTFIHIVLPQAIRQVYPIYKGELIALTKLTSIVGYIAVQDLTK
                     ASDLIRSRTFDAFFPLIVISVIYFFISWGLARLLDAALPTSIRHS"
     gene            complement(13382..14347)
                     /locus_tag="PLM68_00075"
     CDS             complement(13382..14347)
                     /locus_tag="PLM68_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT53799.1"
                     /translation="MAGKGSKIAKKLRGSAGFYAAIAIACWSTVATAFKWALEGLTPA
                     SMLFIATTAAIATLFILLRAGGYRGDFFAIPWKAWKRSAFLGLVNPFLYYLILFKAYS
                     VLPAQVAQALNMIWPLILVLLSIPILGQKIRWAGILAMVISFAGALVIALQGDPLTVF
                     REGLLIKNPWGVVLALGSAFLWAVYFLINMKNTLPDEISLLMNFLFAWVYIALFLLLS
                     GLVSLRNGTGPASPALLKSILSGIYIGIFEMAVPFVVWLKALKLAKNTAVVSSLIYLF
                     PFLSLILIHYVLGEPIFPSTVIGLILIITGVLLSRFSNRSAPKSI"
     gene            complement(14323..15858)
                     /locus_tag="PLM68_00080"
     CDS             complement(14323..15858)
                     /locus_tag="PLM68_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOT53800.1"
                     /translation="MFKGQPKGLIAAALANMGERFGFYTMMAILSLFLSAKFGLSETT
                     TGLIYSIFYALIYILALVGGVIADKTKNYKGTILTGIIMMAVGYLLIGLPTPTPVPNL
                     PLYLTITCVGLFTIAFGNGLFKGNLQAVVGQMYDNPKYADKRDTGFQIFYMFINIGAI
                     FAPFFAIAVRNWWLQSHGLFYNAELPALCHQFRAGELTGEAMDKFALAATEATQGPIT
                     DLAAFADRYLNVFSTGFHYAFFVAIVAMVISLVIFITTKKRLPDVKKDGAAKDKTAST
                     VTKMDVAEVKQRLLALFAVFAVVIFFWFSFHQNGLSLTFFARDYTDLSMIDIDLGFTR
                     IEGAEIFQSVNPLFVVLLTLLIVPFFSWLNKKGMEPSTPKKIAIGMGIAAAAYLIMTI
                     ASINLPLFSEVKAAGGLEQSAKVGPFILISTYFVLTIAELFISPLGLSFVSKVAPPHM
                     QGLMQGGWLAATAVGNQLLFIGGLMYSHIPLWATWTVFVVVCLISMITMLSMVKWLER
                     VAK"
     gene            15924..16850
                     /locus_tag="PLM68_00085"
     CDS             15924..16850
                     /locus_tag="PLM68_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT53801.1"
                     /translation="MKALFSQLGYFALYTIVFLLSLLPLPVLYGLSRVLVVLSVNLAG
                     YRRTVVISNLTRSFPEKTKREILIIARDFYRYLGRLCAETIWLFTASQQSVMKRFSLE
                     GCEVPESLKRMGKSVVVMLPHIGNWEYLRACSCPGFGSVCGFDQDQVHMVYQQVNSPL
                     FDKLMYTLRNRYKTGWHLLESRRAGSHMARHRDVQGIYTMIADQCPGPAGKSYAVSFM
                     GQPTLMIAGPETLARKFNFAVVYGVIRPSAKGHYMLHFTLISENAAVHEEGYVTRRFA
                     QLLEQDIRKNPHLWLWSHKRWKRTPGQGSIFA"
     gene            16871..17920
                     /locus_tag="PLM68_00090"
     CDS             16871..17920
                     /locus_tag="PLM68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOT53802.1"
                     /translation="MKQLIILLLTISLWVPLQGQPVTEGQQIPPGAVVYSLPRTCVRI
                     VAEAVYVNFNPGPYARYARKFLGIDAGQAKTTTYELSRVEMTPMLEADPHQLYVVNLS
                     TANAQPWFLQLTSQGLVLLSDAYASKENNWRFPSLDVSPDVSDAGIIENLKEERTTLY
                     RSQVRGREVSAIPVQQTQLVEKTLEQRAEEAARKIFELRNMRFAIITGDTDAVYSGEA
                     MGAAVEEMRRMEEEYLKLFLGETTTGKTHLSTDVLPDPDNTRYVAFRFSETEGLLPSG
                     NVGGRPILLEIDTSAEETIPRLLADESRIRTGLRLYYRQPVITLVRLTDGQKELLQAR
                     IPVYQKGPVLNFIIN"
     gene            17932..19389
                     /locus_tag="PLM68_00095"
     CDS             17932..19389
                     /locus_tag="PLM68_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT53803.1"
                     /translation="MDIINLKPQRVWKHFYSLTQIPRPSKKEEKIRAFLAEFGRNLGL
                     ETIVDETGNVIIRKPATPGMENHKGVILQAHMDMVPQKNNATEHDFEKDPIIPRIEGE
                     WVYATGTTLGADNGMGIAAAMAVLEARDLKHGPVEVLITTDEETGMTGARGLKPGVLQ
                     GDILLNLDSEDEGELYVGCAGGEDGSFEMEYQPSPVPAGWKAARINVTGLKGGHSGMD
                     IILGRGNANKIMTRILYHLLQECNICIAWAEGGNLRNAIPREADAVICFPSDDAACVK
                     GKVENMAGIIGNELKQVDPGLEVNVTMCDIPAHVIPSEIALKIIRAVYAAPNGVWGMS
                     ASMPGLVETSSNLAIFKAKDGKMAVHCLIRSLADTAKQDMGTALACCFANIGASVSFS
                     GAYPGWRPDMDSPILKVMLDTYKKMYKQEAKVKAIHAGLECGILGGTYPHWDMISFGP
                     TLRSPHSPEERCLIPTVQKFWDLIVETLQNIPEKQ"
     gene            19485..19907
                     /gene="rpsF"
                     /locus_tag="PLM68_00100"
     CDS             19485..19907
                     /gene="rpsF"
                     /locus_tag="PLM68_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT53804.1"
                     /translation="MQQYETVFILTPVLSDAQMKETVTKFRDTILANEGEIVYEEDWG
                     LKKLAYPIQKKTTGFYHLIGFKANPDFIARLELQFRRDERVIRYLTFAMDKHAVAYAE
                     KKHGQVKDAAPVVEKEEKPAPRVTKPVVDDDIEIDEED"
     gene            19911..20177
                     /gene="rpsR"
                     /locus_tag="PLM68_00105"
     CDS             19911..20177
                     /gene="rpsR"
                     /locus_tag="PLM68_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT53805.1"
                     /translation="MAQNNEIRFLNPPTVEVKKKKYCRFKKAGIKYIDYKDPEFLKRF
                     LNEQGKILPRRLTGTSLKYQRKVAQAVKRARHLALLPYVTDLLK"
     gene            20189..20641
                     /gene="rplI"
                     /locus_tag="PLM68_00110"
     CDS             20189..20641
                     /gene="rplI"
                     /locus_tag="PLM68_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT53806.1"
                     /translation="MEIILKQDVEKLGHKDDIVKVRNGYATNYLIPKGMAVLATPSAV
                     KIHQENMRQRAHKETRIREDAQALAAKLAACQVRIATKVSSNGKVFGSVNNIQVAEAL
                     AAQGIEVDRRNITFTGAESLKEVGVHETVIKIYRDIIATIQVEVVGEE"
     gene            20716..21996
                     /gene="glyA"
                     /locus_tag="PLM68_00115"
     CDS             20716..21996
                     /gene="glyA"
                     /locus_tag="PLM68_00115"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT53807.1"
                     /translation="MKQDITLFDLIRKEENRQTNGIELIASENFVSPQVLSAMGSVLT
                     NKYAEGYPGARYYGGCEVVDKSEQLCIDRICELFGAEYANVQPHSGAQANMAVFLACL
                     QPGDTFMGLDLAHGGHLTHGSPVNFSGILYRPVAYTLNPETGVVDYDQMERLALEHKP
                     RMIIGGGSAYPREWDYERMRGIADKVGAIFMVDMAHPAGLIAAGLLDNPVKYAHIVTS
                     TTHKTLRGPRGGIILIGKDFDNPWGITTPKGVVRKMSSLINSSVFPGVQGGPLEHIIA
                     AKAVSFFEALQPEFRDYQVQVRKNAQALASAFIRRNYTLVSGGTDNHLLLIDLRSKFP
                     ELTGKEAENVLVRADITVNKNMVPYDSRSPFKTSGIRVGTPAVTTRGLKELDMETIVA
                     LVDEVLSNLDDQAVIDRVKRKVKELMGGLPLYAW"
     gene            22008..22724
                     /locus_tag="PLM68_00120"
     CDS             22008..22724
                     /locus_tag="PLM68_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53808.1"
                     /translation="MKRNTLTIALLIAVALLLCETSGAQVQKRGYTEAVIGTDINDIL
                     PDDMIFMYPEFLKGSVHFLDRSVAEGVMNIYLPGLQLYFIDSNKDTLVMRNQDEARLL
                     TIGHDTYLRHDKTWVRILATSGTAAFCLKSVLKIHQDVKIGAYGTTDVTSSITNVDMM
                     YGVEGNTAIRLKSLRHVPYTLTHFGLLYDGSKLFIANVRNFKRMFPDRKDDIDKYIAE
                     NKLNLNDAKNALQLFNFLNL"
     gene            complement(22701..24917)
                     /locus_tag="PLM68_00125"
     CDS             complement(22701..24917)
                     /locus_tag="PLM68_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOT53809.1"
                     /translation="MKKHRVLLLFLFLAGLITARSNTVLTIRQERTEEDKVHLISAQM
                     AEMYELFGVSYRKITGPARFLHNNTYILCDTAIWNTAENIIDAVGNIQIIQEGTRLTG
                     ETIHYLGDLNTAEVRGRVVELIDKEENRLRTQYLNFNTKDSIAYFFNGGSVVDNEGTI
                     LESRRGYYYSKEKLFCLYEHVEMGTDTLRIKADSLYYHSDSNLAEFWGNVQAWHTDGF
                     LTSREGTYHRDEELYHFLDDVFIRSDEQEIRAEDVLYNKGSNSGILRNNVQVRDTTQQ
                     IHILGDRLDFRREPDWVRVTEDPVLVVWEIDSETNRRDSLFLRADTLLAYTSTYGKMD
                     SLERVRAFERQEIPERKRPAAAPDSIILPDSSSHAMPGSVNLPGSPALPEKDSLPPAK
                     DSLPSGRDTIPDPTAEAAVNYLRQNLRDVQVFLVRADSTLLADVKILGDSLFNTTDVH
                     FLYGYNNVRVYKSDIQSICDSLVFNSIDSVVRQYGKPVLWNEESQLSADSIQFRFSRD
                     SLYRVDFLSAAFLISQEEQVFFHQIKADLMYAHISDNDVNRFDALKNTRALFYIPEDS
                     IITSLNIKECEEMKVILKDRRAQRITYKKSVKSDLIPLYDLTKEQERLSGFDWRGAER
                     PLDRTSITSRTIEETFSEPGIIYKEPLFPYTLKYFPFVHALPSTLRTGVTTDAVKELS
                     QRERENKVNPYIPLKEIPAAPAQQDNIPAKRDTIPAQTRRIRPVQERRQLQIQEIE"
     gene            complement(24917..26281)
                     /locus_tag="PLM68_00130"
     CDS             complement(24917..26281)
                     /locus_tag="PLM68_00130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT53810.1"
                     /translation="MKINKIASSLLFFLSITTFAFGQKYANGLVDKVIALVGNEMIQL
                     SAIEEEVQMQIMQGIITDKNLRCEILENMLVSKLMLNQARLDSLTVNEEYVELELENR
                     LQEIKTRLGGEKATEDYFHKPIFKLKQEWRDLFREQSLTRDMQYAITDKIPDMTPKDI
                     EDFYDRTSKDSMPIIPTQYQLSQIVLYPPKEEAILLVKERLLEFRERIINGERFNTLA
                     TLYSQDPYSAMRGGELGMAAKQMYWPAFGDAAIVLKEGQVSQIVETPDGFHLIQMIER
                     EGDMFNARHILLKPDVLTADKLKCLGRLDSIATAIKSDSLTFEKAAMKYSGDPPSRLN
                     GGRMSDENSGSMLFDKDQLKTNDYNVLKDMKEGDISFAFESRDNEGRDGNVIYKIIRL
                     DKIVPSHVANLQDDYYIIQNMSKEKESQNAVERFIKEKQQTTYIRIDPLFRDCPFKRE
                     GWIK"
     gene            complement(26271..27137)
                     /locus_tag="PLM68_00135"
     CDS             complement(26271..27137)
                     /locus_tag="PLM68_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53811.1"
                     /translation="MKKWILILLLLTTLTSCHQIRRWLGNEPERVAQIGKEILYIDEV
                     EGLLKSAATSDDSLNLVNHYIDLWAIDNLLLKKAEADLSKEDKDVDQELADYRKSLLV
                     FRYQKKYVEERIDTVIEQEEILAYYQDNLELFLLDDPLLKLRLIKMRLHSPYLPMVRN
                     IYRSNTIEDQTQLRRLCESSAEIDTDYEGKWLTGQELAQDLPVGNMDKIQALKADGYI
                     DTRDSLYAYLVRVYDFVPAGEPAPIEQTEARIRQIIIIKRKQKLLEDLEVEVLKEGWN
                     KQMIKIYRKNED"
     gene            complement(27146..28615)
                     /gene="guaB"
                     /locus_tag="PLM68_00140"
     CDS             complement(27146..28615)
                     /gene="guaB"
                     /locus_tag="PLM68_00140"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT53812.1"
                     /translation="MKSISDRILYEGLTFDDVLLLPAASAVLPREVDLTTRFSRNIIL
                     STPIVSSAMDTVTDATLAIAIAREGGLGVIHKNMTIEQQMEHVRKVKRSESGMIYDPI
                     VIGPEATVGDALRLMAENSIGGIPVVDSKHRLIGIVTNRDLRFVNNDSTPITEVMTKE
                     NLITIRNGADLSTATELLKKYKIEKLPVVDENYKLIGLLTFRDIRKIKDNPRATKDSK
                     GRLRTAAAVGVRANTLEHIEKLLSVDIDAVVIDTAHGHSISVLETLRKAKKAFPHLDI
                     VAGNVATATGALALAECGADAVKVGIGPGSICTTRVVAGVGVPQLSAIMGAYSVLKDK
                     GVPVIADGGVRYSGDMVKALAAGADTVMAGSLFAGVEESPGETIILNGRKFKSYRGMG
                     SLEAMQQGSKDRYFQDAEEDVKKLVPEGIVAQVPYKGTLSEVFYQLAGGLRAGMGYCG
                     TPDLASLKKAEFVRITSAGVIENHPHDVTITREAPNYSR"
     gene            28678..29859
                     /locus_tag="PLM68_00145"
     CDS             28678..29859
                     /locus_tag="PLM68_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOT53813.1"
                     /translation="MKKWKIGLLARVAIAIILGILSGLFFPTWALRIFLTFNGLFSNF
                     LDFIVPLIIVGLIAPGIAELGKGAGKMLGTTMALAYGSTVLTGFFTLLACKLVYPILL
                     DGAETAMTMASPAENGLSAGEQLVPYFTLDFSPLFGVMPALVLAFVLGLGLTIINNGD
                     VLKQALLGFREIVNKTISGVIIPLLPLFIFGIFMKMAAEGHVVQVMGVFVKIVILIFI
                     LTVILLVFQFSVAGLVARRNPFTMLRTMLTAYVTALGTQSSAATIPVTLHQTISLGID
                     PRIAGSVIPLCATVHLSGSMLKITACALAVMIMMNIPVDPAVFVGFIFLLAITMIAAP
                     GVPGGAIMAAIGVLQSVLGFDPEATGLMISLYIAMDSFGTACNVTGDGAIAVIVDKMY
                     A"
     gene            29852..30385
                     /locus_tag="PLM68_00150"
     CDS             29852..30385
                     /locus_tag="PLM68_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOT53814.1"
                     /translation="MRRVSVFCGSHTGNDPGIIQQAELLGSILAEQGIELVYGGTNIG
                     IMGVLANSVLACGGSVEGVMPSLIEEKRIAHHHLTRMVLVSTLEERKKKLIEDCDAIV
                     VFPGSYGTLDELFSALVLKQLGKIDKPIVMLNFRGYYDPLLAQLDHMVKAGFMPERFR
                     SNLQAVEDCHQLHFLWD"
     gene            complement(30312..31304)
                     /locus_tag="PLM68_00155"
     CDS             complement(30312..31304)
                     /locus_tag="PLM68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775449.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT53815.1"
                     /translation="MMQRYKSFTGYFISRYGTRLQKVVVDAGFSCPNRDGTTGTGGCT
                     YCDNRAFHPGYSTPDKSLTLQIDEGIEFHRVRYCKAGGYLAYFQPYSNTHAPLEDLRR
                     IYKPALEDPRISGIVVATRPDCIDARKLKYFRDLSLEKIVILEYGVESCYNRTLQRIN
                     RCHTWEQSAAAIRDTAALGIHTCVHMIFGLPGESREEMLSEAGILSRLPLTSLKLHQL
                     QIVKGTPMEKEFAEKPGDFVTFSLHQYLDFIIDFLERLRPDIFIERVAGEVPPRFVHS
                     NPWGLIRNVEILRLLEKRLEERNTFQGALYVNPTESATDGNPQPPAGLNENAPA"
     gene            31361..32572
                     /locus_tag="PLM68_00160"
     CDS             31361..32572
                     /locus_tag="PLM68_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOT53816.1"
                     /translation="MKKQFYYLVAISAALAVLAGCTKTRTLEKLSNISDNVKTAFTPD
                     RRDNVYEIVFVKLKGSKTYVMKGSTTEPGVVPALVAAAASQGIELTDSIVLLPDPALE
                     GRIYGVTSLSVANLRYEPRYSSESATQTLMGMPLRILDKKGSWTRVRTPEGYIAWVSS
                     SSLQEMDEQTYGQWMKAPKVIVNKHYTLFREKPLPGAPVVSDGVWGNIVELEGQTPLF
                     YKVRLPDGKQAYLPRYDAEPFEAWARSGNPLPEDIIATAMQFVGFPYLWAGTSIKAMD
                     CSGLVKTAFYLNGIVLLRDASQQARMGEQVDISEGWQRLTMGDLIFFGRKATEDRKES
                     ITHVGIYIGDGRFIHSAGRVRINSLDPASDIYYEGSDRLLRAARILNHIDDGTQVVSV
                     REHPWYGIKTQ"
     gene            32596..33774
                     /locus_tag="PLM68_00165"
     CDS             32596..33774
                     /locus_tag="PLM68_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOT53817.1"
                     /translation="MNSTRRDFLKTSMAMAAAAALASPSRLFAGNAKKTLQSKKAASS
                     DKMVLTFEPFEARMKHVFTISGSSRSTTPIVLTRITYQGHVGYGEASMPPYLGESTAS
                     VMEFLSRVDLSRFSSPFQLEEILTYVDNITTNNTAAKCAVDIALHDLCGKLMGQPWWK
                     IWGWSNKNLPSTCVTVGLAYAPDGSIDREEVRKKTLETLDYNLIKVKLGNTEENDKTM
                     INTIREVTDLPIVVDANQGWKEKGYALSMMEWLAARGVEMVEQPMPKLLLDEAAWLRD
                     RSPLPIMADEACQRLSDVRKLYGAYDGINIKLMKCTGMREAREMITLAKALNMRIMMG
                     CMTETSVAISAAAQFASQLEWADLDGNWLIANDIFTGMKVVDGYITLEDKPGIGVEPL
                     "
     gene            33794..34069
                     /locus_tag="PLM68_00170"
     CDS             33794..34069
                     /locus_tag="PLM68_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53818.1"
                     /translation="MPENLRTLKKDFDYLIDEVISDCCTFVYVNPDKKEAEAMDVINE
                     AIALRDEMFERLNKIREKPAKPHIKALKEEFLTRIDALFEKISELAK"
     gene            34086..37601
                     /gene="metH"
                     /locus_tag="PLM68_00175"
     CDS             34086..37601
                     /gene="metH"
                     /locus_tag="PLM68_00175"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOT53819.1"
                     /translation="MSGRILILDGAMGTMIQRFAPSFPPGNDGNNDILTLTQPELILS
                     IHREYVQAGADIITTNTFNANAISQQDYGMQDQVYTMNFQAARLARESDARFVAGSLG
                     PTNKSASISPDMNRPGVRATDFATLVGAYREQARGLKEGGVDLFLVETVFDALNAKAA
                     LFALSEEAPGMPVMLSATIADNSGRILSGQTLEAFLVSTAHAGLFSVGLNCSFGPDKM
                     LPYVRILSRFAPGFTSAHPNAGLPDMFGKYTLSPEAMAQQVKPYVEEGLVNIIGGCCG
                     TTPDHIEALAGMVRKNQAPLRRVPVRVEEGQPQRITLSGLEMLTVREEPAFLIAGERT
                     NVSGSRKFARLIREKQYDQALEIARGMVRDGAHILDVNMDDPMLDAPTAMREFLFMLG
                     SDPEVARVPLMLDSSRWEVLLAGLQCVQGKSIVNSISLKEGPEAFLRKAKLIKQYGAV
                     PLVMAFDEQGQASTRERRIEICTRAFRFLVDQAGFEPHDIILDPNVLAVGTGMEEHRH
                     FAVDFIDTVRYLKQNLQGALVIAGVSNLSFAFRGNETIREAMHAVFLYHAIAAGLDMA
                     IVKPGAQPIYSEIPEPLRRAVEDVILDTDAGATSRLIEMAATINGPGQQEAGTVTREA
                     WRDLDAEERLHHALAHGIEEYLEEDLEALCTTPAIDIVEHTLMEGMKRVGKLFGEGKM
                     FLPQVVRTARTMKRAVSILQPRIEAEKNSKTSGNAATETVKGIIATVRGDVHDIGKNL
                     VILVMECNNYKVSDLGVMVPAHKIVEAAIREQAAFVGLSALITPSLDEMVDVAGAMEK
                     AGLTTPLLIGGATTSSLFTSLRIAPLYSGPVIHCPDAAGVVPVLAACTSKDPVERERY
                     FTDLRASQKRMRDAYGMAAARKEYLSPAEARANKWVPSLSLAGQESCPCCGQHRVPDD
                     FVPPFMGVQHVEIPFAKLRKHLDWDTFLHIWEVRESREQGKIAVEDAVRLLDDLIGGH
                     YGDIAKVRAAAGFWPAVSTPDDHILFFAGDSSLHIVLDLPMERDLLRHADGSPNTSLA
                     DFIAAADPAHTPDHAGLFLLTASSPQLEKLAESMQKRGDVYNALILRTLLDLLAEAAS
                     EALHRELMVFSCGAPRGIRPAFGYPSCPDHTLKQSVVTMLQPADELGIALTSSCMLQP
                     AASVCGMYISHPQAHYITVHKDPGL"
     gene            37598..38470
                     /gene="metF"
                     /locus_tag="PLM68_00180"
     CDS             37598..38470
                     /gene="metF"
                     /locus_tag="PLM68_00180"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOT53820.1"
                     /translation="MKLTDIFKVSEKTFSFEFYPPRDEIAAVDFGLNIGQLIKLSPSF
                     VSVTYGAGGSTQSRTFDLVDYLKNRVGLNTVAHYTCVGSTRSRTLEDLKTLRKLGLQN
                     FMLLRGDPEVGSKYGFIPCYDGFANATELITFAREFIDDCCIGGGAYPEVHPESPSAE
                     IDMRNLKKKVDAGCDFLITQFFFDNKRYFDFVSRARKAGIKCRIIPGIIPLTKYSQLE
                     RFVNLSRASVPPAFLEQLESNRYNPEAVYKAGMDFAIRQCRDLLMLGAPGLHFYTLNK
                     SRATVEIYQTLMVR"
     gene            complement(38449..38982)
                     /locus_tag="PLM68_00185"
     CDS             complement(38449..38982)
                     /locus_tag="PLM68_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT53821.1"
                     /translation="MPRKIPNRELRRLSPGEFRNSPRMPVVLVLDNVRSQHNIGSAFR
                     TADAFALEHVFLCGITACPPSAEIHKSALGAENTMEWTYVENTLDAVRRLQEQGYAVL
                     AVEQAEGAGNLAAFSPGKHQKTALVFGNEVRGVKQEVVDACDGCLEIPQHGTKHSLNV
                     SVAIGVVLWHYYLTMRV"
     gene            39020..39619
                     /locus_tag="PLM68_00190"
     CDS             39020..39619
                     /locus_tag="PLM68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOT53822.1"
                     /translation="MPTMYVISGCNGSGKTTASYTILSELLQCRAFVNSDEIARGISP
                     FDLSNAAIDAGRVMLKRVRELSESKEDFAIETTLAVRSFASLIEKTKKKGYDVMLVYF
                     WLKSPDLAVKRVQSRVMAGGHTIPENVIRRRYWSGIQNLFNIYIPLSDRWLLINNSDT
                     PSSLIAEGHGHREIKIFNPEDYQTLKKLSQNPLPGSGKP"
     gene            complement(39573..41573)
                     /locus_tag="PLM68_00195"
     CDS             complement(39573..41573)
                     /locus_tag="PLM68_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOT53823.1"
                     /translation="MKTKPATGMLTLAVFTFLTCVTGTILQAKVKVHSISSPDGKLEL
                     QAVTEPNISFCLLRGDQVLVSSPALSLSLEDGTILGPNARLSRAKTTNVREIIPSPLY
                     RKSEIIAVYNQLDISFREGFGIRFRLYDQGAAYQFYTTGRDSLHIVNESAGFVFEKDY
                     TCYVPYSRGVESPFNNSFENIYTVTPVSGFNPDSLAFLPLLVCLDDGIKMVLTESDLE
                     SYPGMFLRGEKTSRGFEYRGVFAPIPSATRTEPVRGQVIPTDYSRVIARTPGTRSFPW
                     RIMAIAEKDTELPVNDLVYALGAPNRTGATDWIKPGKVAWDWWNNWGVTGVDFPVGIN
                     TETYKYYIDFAAANGIEYVVLDEGWSPPAGRNIMQVVPQIDLQELVTYAEKKNVGLIL
                     WCVAYVLDEKLEEACSFYSRMGFKGFKVDFMDRDDQPVVEMLYRIAETAARHKMLIDF
                     HGMYKPTGFNRTYPNVVNFEGIFGLEQSKWSREDMVPHEVTFPYIRMMAGPVDYTQGA
                     MRNATRQDFHPVYNNPMAAGTRARQVATYIVFDNPLVMLCDNPSTYMKEQETTSFITC
                     IPVAWDETRILQGELGKYIVSARRKDSQWFVGGLTDWTPRTITLDLSFLEPGRTYRAK
                     IFRDGVNAHRHATDYRIEEREVTSKDEIKVYLSPGGGFAIIF"
     gene            complement(41575..45372)
                     /locus_tag="PLM68_00200"
     CDS             complement(41575..45372)
                     /locus_tag="PLM68_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT53824.1"
                     /translation="MLRYLFVSIILSLIPSLSLWSQPGIIAHRGFWRAEGSAQNSRSS
                     VQNAIDARCYGAEIDVYLTTDGKVVLVHDPVLNGVRVDASSYAELSGYRLSNGETLPL
                     LDEILALIAASDHTRLIIEIKSHRNQDTEKAAVARILELVSQAGVRNKVEYISFSFHI
                     CETLIHSDPEARVAYLGGNLSPDQLKEKGYTGLDYNMRVLKAHPQWIPRARELGLDVN
                     VWTVNRPEDFHYFIQSGVDFITTDYPLYKAGEVWQDPEVNQINRVPMRTSYHAYESRS
                     LALDGKRESSRYFKDLNGLWKFAWTHDANGGYTGFEQSCYDDRSWEIIPVPGLWELNG
                     YGHPLYLNFNYAWYNQYRSAPPVVPLENNHTGYYRHNFSLPEEWNGRQVFIHFGSVTS
                     NMSLWVNGMFAGYSEDSKLEAEFDITPFLRPGDNLIALKVNRWCDGTYLEDQDFWRLS
                     GMGREVFLYAREKTHMHDFILHPVLDDNYRNGTLGMSVTLNSPSAGTRVRAELIDPQG
                     VSLGQKTLSSGESNTYTGSFTVKNTKNWSAELPHLYTLLLSLDDTETGKETEFIPWKV
                     GFRSVEIKNAQLLVNGKPILIKGVNRHEMDPATGYVVSEERMIQDIALMKRFNINAVR
                     TSHYPNSPLWYRLCDQYGIYVVDEANIESHGMGYGERTLAARTDYQRAHAERMERMYL
                     RDKNHTSVIIWSMGNEAGDGENFTMGYDLLRKADIQKRPIQYERATRPEISDIFAPMY
                     MGYESCIRYLENNPQRPLIQCEYAHAMGNSMGGFDYYWEIIRKYPQYQGGFIWDFVDQ
                     ALFLDRKEGHYVYAYGGDFNPYDASDQNFNNNGLFSPARVPNPHAYEVGYHYQNVWVQ
                     DVDVANGRIGVMNEYFFRDLSHLSLEWELVANGVAVKKGYADDLKTPPGETSVVDLGY
                     RKTFLSSYSDQEVFLNVYFSTLQAEALLPAGIVLARAQLPVHSPEPAKRPEEGQEVQS
                     FTMEHNDRNWLIARSGNMRVEFSRRDGFVSHLSFSGISILDENSRITPNFWRAPTDND
                     FGALLQLKYVAWRDPVMEFKGFETTDDAITAVYDLPQLKSRLTMTYRTAPQGGLQVTQ
                     ELEALATAEEAGKIPPMFRFGVRFNTPKKYNRLIYYGRGPGENYPDRSAAAFTGLYRQ
                     SVSEQFYPYIRPQENGTRTGLRWWELLDHTGSGIRVDSDSLFSASALHYSIESLDSGP
                     AKRNLHSHDLKEENFTQCCIDLRQMGLGCVNTWGALPRPVYMLPYGNYAFTFTISETK
                     NNY"
     gene            45416..46003
                     /locus_tag="PLM68_00205"
     CDS             45416..46003
                     /locus_tag="PLM68_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT53825.1"
                     /translation="MRIYDISQEIFSGNIFKGDPVPAATPLYRIQNGDPYNLSSVSMC
                     LHSATHIDAPSHYLEDGLTIDQVRLEHCIGPCTVLSVNGPVDEGLITPLLKRGAKRLL
                     FKGEGWLSVKAARALAESEVVIVGTESQTVGYGNYIDDVHRELLKSNIVILEGLVLQD
                     VPEGNYYLISLPLKMAGMDGSPCRAVLLDYMPPVD"
     gene            complement(46000..47052)
                     /locus_tag="PLM68_00210"
     CDS             complement(46000..47052)
                     /locus_tag="PLM68_00210"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOT53826.1"
                     /translation="MEVTQYIHGYSPKGEAIVSYKITNKKGSYVQLCNIGATVMGIGV
                     PDRNGLVEDVVPGYQDVMQYFLDPPYLGKTVGRYANRIARGLFSLEGKQYQLAVNNGS
                     NHLHGGPQSMCVQVWVSRIEDGDDAVSFTYASPHGEENYPGNMQVTATFRWSENDVLT
                     LELRAISDLSTIINITSHTYFNLAGEGNGTIHNHILQLNANNYLPVDPTSIPLGDPAP
                     VKGTPFDFTVPKPIGKDIAMDNEQLRIGNGYDHCWCLPSSDGTLQQAALLKDPVSGRT
                     VTISTTQPGIQVYTGNWLEGNGKGKNGIPHRNRHAVALECQNYPDSPNRSQYPDCILR
                     PGELYLQKIVYAFGTD"
     gene            47087..48286
                     /locus_tag="PLM68_00215"
     CDS             47087..48286
                     /locus_tag="PLM68_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT53827.1"
                     /translation="MKKALKISTCFFALLLASCQPYHDAPSGPDQYLAGIHKIIDLNL
                     DELFNTFGVEDYPEITSKLEYVISSRNFRAVVITYHTTDPFGEPVIADGTLYYPLDCD
                     IRGVVEMSGIAHMSKDGGSSENIPVLEALPVLVGYAVILPDMIGYGKYGNTKEMPHPF
                     MICDNLGKVAWDMRMAATEYFRTLGYNVPRHTIISGYSYGGGVGLAVARYYQEHHMHE
                     VYVERVVIGGGACDLNATFKGFADNPICTYPLLPGIIMSLDHYYNLSLDYHKIFQGPL
                     ADNYALWYDRTRIASGLVSLIGEDMRNYMHPDFFKPAEERNSEFQKLQPVLDMNSAVD
                     GWVPYMPVYLYHSTEDQYVPEEAANYAYRQFRKAGAYIQYKSGPGTHADWGVETFAGL
                     LLHLAIK"
     gene            complement(48279..49502)
                     /gene="pepT"
                     /locus_tag="PLM68_00220"
     CDS             complement(48279..49502)
                     /gene="pepT"
                     /locus_tag="PLM68_00220"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOT53828.1"
                     /translation="MEKILDKFLRYISFDTASDPQSETQPSTTKQCALLEVLLKELHQ
                     MGITDAHLDEYGYVMASIPSNLEKEVPAIGFLAHVDTSPDASGKDIRPQIIPKYDGKP
                     ILLNKEKELYLDPAEFPELLSYQGQTLITTDGNTLLGADDKAGVAAIMSAAEYLMTHP
                     GIKHGPVKIAFTPDEEIGRGVAKFDVKKFGAAYAYTIDGGQLGEMEYENFNAAMASIH
                     IQGRNIHPGYAKGKMVNALIIGTELNNMLPVEQRPEFTDHYEGFFHITSFRGTVEEAS
                     LIYIIREHDHGKFEQKKQLMQDVAGFLNLKYGNCITLEMKDQYFNMRRQIEPHMHVVE
                     KAIKAIKAAGITPIVHPIRGGTDGAQLSYRGLPCPNIFAGGHNFHGKMEYLPLKSMEK
                     ASKVILRIIDEYAVT"
     gene            complement(49514..>49997)
                     /locus_tag="PLM68_00225"
     CDS             complement(49514..>49997)
                     /locus_tag="PLM68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOT53829.1"
                     /translation="GFTGENALVAPQANAMAAVIEPLMSGGGAPWLLYGIGGLIAILL
                     TLFKVPALAFSLGMFIPMELNLPLLVGGAVAWFVSTRSKKSLVNEERKDRGTLLASGF
                     IAGGALMGVVSAIMRFAGINLVNAEWQHSTAGELLALAAYACIIIYLAISSMRAAKER
                     "
BASE COUNT        12351 a        12286 c        12947 g        12413 t
ORIGIN      
        1 tgttgtttcg gttgccggtt gtgttctcct ggaacacctg attgtccggt accttatgag
       61 gaaaaaaaca acagtacatg acctctccgc tgtggtcacc ggattgatac tggctgtaaa
      121 cctgcctgta tcctccccat ggtggatggt actgatagga tgtattgctg ccatcgggat
      181 cgcaaaaatg accttcggcg gattgggcca caacttattc aatcctgccc tggtcggccg
      241 tgttttcctg ttgatctctt tccctgtaca gatgaccaac tggtctgtga acaacagcat
      301 tttcagctcc gagacatgga atctgcccaa aatactgctg ggtgcccagg ggcaattgcc
      361 cgatgcggtt tccggggcca cacccctggg aatcatcaag gaagggctgg ccaacggcct
      421 gacgatggaa acattgagcc agcaatacga tttttcttac tggcaaatgc tcttcggaca
      481 tatagacggg tctttgggtg aggcatctgc actggctgtt ttagtggggt ttgcgtacct
      541 gctcttcagg aaagtcatca aaccccttat ccccgtttcc atacttttca ccgtggctgt
      601 tttttcagcc atattgtggc tgctggatcc atccatgaac gccgaccccc tcttccacct
      661 gcttaccggg ggattgctcc tgggcgcggt attcatggca acagactaca ccacttcacc
      721 catgtcgccg ccgggaatgc tcatctatgg ggtgggtata ggggtgatga ccatgatcat
      781 ccgtgtctgg ggagtctatc cggaaggtgt ctcttttgcc atattgttta tgaatgcttt
      841 aacgccccta ttaaacaaaa tcaaaccaat cccatacaaa aaggaggtga gccgtggcta
      901 gggaatcgaa tttcaaaaac atgttcctgt gcctgctgat aatctgcttt ggcggatcgg
      961 cactgctggg aggtgtatac ctgatcacgt acgagcccat tgaggcagct caggttgcta
     1021 aggtcaacca ggcactgggc ggtgtattac cccaatttga caatctgcct gcccaggaag
     1081 cgtatgaagt ggaagtggga ggacgaaaag cgaccgttta cccgggcacc aagaatggag
     1141 aacttaccgg ggtggccgta gaggcaacca ctaccaaagg ttttggaggc cagatcaagg
     1201 tgatggtggg ctttctgccc gatggcacta tcaacaacac ggccatcata tcgcacaccg
     1261 aaacccctgg actgggagat aagattaatc ccaaaaaaag taaatttcct ttgcaattca
     1321 accagaaaaa ccctgaatct ttccggcttg ccgttaaaaa ggacggaggg gatgttgacg
     1381 ccataacagc ggccaccatc agttcaaggg ctttttgcga tgtggtggac ctggctttcc
     1441 aggttttcag gaaatcaatt caacaagaag gaggtgacca atgagcagga tgaaaacttt
     1501 agtgaacggc attataaagg aaaatccaac atttgtgctt gtcctgggca tgtgtcccac
     1561 actggggacg accacttctg ccataaacgg gatgagcatg ggcctggcca ccacttttgt
     1621 gctcatgttg tccaatatgg tgatttccct ggtatcgggc ctggtcccgt caaaggtccg
     1681 gattcccgct ttcgtggtag tgatagccgt ttttgtcacc gtggtgcaat tggtcatgca
     1741 ggcttacgtt ccagcgctgt acgaaactct cgggctgttc attcccctga tagtggtgaa
     1801 ctgtattgtg ctgggaaggg cagaagctta tgccagcaag aatacaccct ttgattcctt
     1861 tctggacggt ttgggttcag gtctgggttt caccctgtcg ctaaccgtgc tggggactat
     1921 ccgtgaactg ctgggttcag gctctttctt tgggttcaaa ctcctggagg gagatggcat
     1981 acttgtcttc atacttgccc ccggcgcctt catggctttg ggatacctaa tggtgttgtt
     2041 caacaagtta aaaaaggctt aatgttatgg aatacatcat catcatcata tctgctgtct
     2101 ttgtaaacaa tattgtgctg aaccagtttt tgggcatatg cccgttcctg ggcgtgtcca
     2161 accggatatc aacggctacg ggcatggctg cagccgtggt gtttgtaatg acactctcca
     2221 ccctgttcac ctacctggtc cagtattata tcctggatcc gctcaacata tccttcatgc
     2281 agacggtgac ctatatcctg gttattgcag cacttgttca gatggtggaa atcattctga
     2341 agaaaatttc tccggcctta taccaggcat tgggcatttt cctgccgctg atcactacca
     2401 actgtgcggt tttgggggtg gccatcctgg ttgtcacaaa agaattcagt ttcgggggga
     2461 caccacatat gctggacctg ccacaatcac ttgtttacac ggcctccatg gcgcttggct
     2521 ttggcctggc cctcatcata tttgccggca tgcgtgagca gatggacctt gtaggggttc
     2581 ccaaaccatt ccgcggcgtt cccatatcac tcatcacggc agggatattg tcccttgcct
     2641 ttatgggttt ttcaggaatt gtgtaataaa gaaagttgtg gaaacaatag aagaactgga
     2701 aaaatacgtc aaaacaacag agggttcccg ggaccagaat gcatggtata agctgggaag
     2761 cttgtaccgt cagcaggaaa actggggcaa ggcactcaat gcctatgcca ggtgtatgga
     2821 actgggcgca tccaacgctg cacgcgaagc gacagaagcc atcaagcgca tcctggatta
     2881 caggaatgag gattggatgc cgtagacaga gaccctgttc ataaggacgg taaaaccata
     2941 atccataaga cgggtagcat cctgggcccg ggcaaaagaa gacggggctt tcatgacaac
     3001 cgcaataaca cgtttgtctg ccagggttgc agtggccgtt atgcaaaggc cggccgctga
     3061 cgtatacccg gtcttgaatc cgtcacatcc attgtaataa taagtttttc cgggcaacat
     3121 gttattggtg gccccataag tgactcccct gaaatccagg ctggtgagtg aagtatactg
     3181 caggatgacc ggaaaccttc tgatgaactc cctggtaagc aacgccatgg aacgaggagt
     3241 gatgccgttc tgtgaagaca tcccggctgc atcgtagtaa cgggcttcca gtttcaggga
     3301 atctgcgtac cggttcataa tcacggaaaa atccgattct gttcccccgg acaggtattc
     3361 acccaaagca acggcacagg cattggccga cactacacat agagccccta tcatttcatg
     3421 gacagaatag gtgaggtttt ggttaagcgg gacgtttgat tctccgggat tgaccgaacg
     3481 tgccgccgcg ttgacactga tggggatcat ggaatccagt gacaggtagc ctttttcaat
     3541 gtgatcaaag acaatgtaca tggtcatcac tttggtcata ctggccggga caagcggaaa
     3601 atcggggctc agggagtaca acgcttcccc gctcgctgca tctatgacta tcgcaccctg
     3661 gcagctcaaa cccagggata ccggtttgtc ggggtctggt tcgggattgg gaaggggctg
     3721 tttttcacag gctgtgaaga ccagcagcag gactgatagc agggcagaca gatagaattt
     3781 catggaatca aaagtaggta aaattccatt acctttgttt tatgaaaaag ttgacctgga
     3841 ttgcattatt gacagccata tcctgcagtt ctcccgatta ttccgtggtt caggacctga
     3901 atacgggatg ggaattctgg aatgaccagg agcctgaagc tattcaggac gtttttcttc
     3961 cccatacacc ccggctggaa ccttatgtgg tgaacgatca gtggcagggg atctgctggt
     4021 accggaaggt ggtggatatt ccaccgggaa ggcacgtact ggtgcgtttt gaaggagcca
     4081 tgaatgtggc tgaactatgg gtgaacggcc attatgccgg aaggcacatg ggaggatacc
     4141 tgccttttgt ctgtgatatt acccgtgtga tgaaagaaaa cggggttttt tcgggacagg
     4201 cggaactgaa actccgtttg gacaaccacg ataatcccat aacagggccc aaaccccttg
     4261 aattgcttga tttcaacatg tacggaggac tgtaccggga tgcccggctg atcatcaaat
     4321 ctccgcttca cattaccgac cccatcctgg aaaacgtgcc tgcaggcggc ggtattctgg
     4381 ttaccttccc tgctgtttca caggactctg ccctggttga ggtccggact catatcaggt
     4441 ttagcccact gaatgatgac caggccgaag cacaaacctt cctcctggaa caggcactcc
     4501 tggccgaaga cagcctgtgt gccttttccc gacaggagat ccgcctggaa agcggtcagg
     4561 cctgtactgc cctagatttg ctcagggtgg agcagcctgc actctggagc ccggaacatc
     4621 cttattgcta taatctggaa acgcggatca ttatgaccgg cccgggggga cgtaccgtgg
     4681 tcgttgacag acagcgcacc accgtgggga tcagggaagt ggtttttcag ggcaaggaac
     4741 tttttgttaa cgggagcaag acttttctgc gcggggtaaa ccgccaccag gagtatcctt
     4801 atgtggggta tgccgtaccc cggaacgcgc aatggcggga tgccctaaag atcaaagaag
     4861 ccggattcaa cctggtacgt ctgtcccact atccgcaatc tcccgatttc atggaagcct
     4921 gcaaccggct gggattattt gtgctggatg ccatcccggg ctggcagtat ttttctgacg
     4981 acccggcttt ccaggaacag gtcataggaa cagccaggga tatgatacgc agggaccgca
     5041 accacccgtg tgtgctggcc tgggaagtct cactgaatga atcgtggatg agtgaagatt
     5101 tcatagacag gatactggaa gcttcccgtc atgaaatgcc cgttcccatc cataccgccg
     5161 gctggcagga atacgaatac gacatcttcc tgcaggccag acagcaccgc ctgggacacc
     5221 cgcccgtcaa aacacccaag cccctgattg tttctgaata cggggactgg gaatattatg
     5281 cgttgaatgc cggatttgcc cagcaaaact gggaaaacct gcaaccggcc gagcggtcca
     5341 gccgccagct cagggaacac ggacagaaac gactgcttca gcaggtcacc aatatcaggg
     5401 aagcgcacag cagcaattac catgtggatg cctgtgccga caattactgg atcatgttcg
     5461 attacaacag ggggtatgcc gatgacctgg aagcctcggg gctcatgtcc ctggaaaggc
     5521 tccccaaatt ctcctggcac tacttccgct cactgcgttc tcccctggag caccccatgg
     5581 tttttattgc cgattactgg gaaggaaccg ataccgctaa tgtggaagtg caggtgttaa
     5641 gcaacgcaga gcaggtggca ttgtggctga acgggaacct gctggagcgc agggacccgc
     5701 cgttcacctt tgtcgtaccc cgctttgaaa aaggctggct gaaagccgag gcatactgtg
     5761 cagataaggt ttgtgcccgg gacagcgttc ataccccggg tgaagcggtt gcggttgaat
     5821 ggtggctgga tacaggtaac atccctcccg ctcccaacga tctgctcttt gtctacgcaa
     5881 aagctgtcga ccgccagggt tacccggtgc catcggcaaa gggccgggtc ttattcaccc
     5941 tggatgggaa agaaatgtgt ctcatcgggg aagagcctga cgggagcgtt tccaggacac
     6001 tccggacaga ggcaccattg cgtgccggaa acgcttttgc cctattacag ctttcacccc
     6061 tttgggatgg aagcgaacct catatcacag taactctttt accatgaaac aaaagatcag
     6121 aatactcatc cccgtattgg cggcagccat cctggcggtt tcgtgccagg gagaagaggg
     6181 atatacagcg tcctattatt tcggatcacg agccaatatg gcctttagca aagtgacatc
     6241 aactcttacg cccggtctta gggagctggc tgaaatgctc acccagaagc gtgacgaaat
     6301 tcaaaaggat tatcaatttg actggacggt gacaagctcg ggcagcaccc gggaaacggc
     6361 tgtacaaaat gctattggaa aggctattgc cagtttcact tccagcatga caatcaactt
     6421 tgacctggac atgcaaagtt ttgccgatga gttttccgcc aaaaaaaagg aattatcaca
     6481 tgctttgtca ggtgaaacgg gttcggtttc gtttacggtg cacttatacc tgactgcaga
     6541 agaaatcata gcagaggcat atgaaatgcc cctggcagtt tccaaaacgt ataccttcga
     6601 atgccggggt aagaacgaat cctactagag tacgggaagc acgctgatga tgcgcacgtc
     6661 cgtgaccggt ctgtcggccg tgcctgtggg caccgatgct atcctgtcta tgatctccag
     6721 tccttcggtc acttccccga ataccgtata attaccgtcc agctggcggc atgtttcggg
     6781 gttatgcact atatagaact gtgacccgga agaagccttt tccggattga cggcatctcc
     6841 cgtcctggca gcagcaatgg ctcctttctt atgggtcagt cctgttacga attctgcggg
     6901 aacggtataa ccgggcccac cagaaccaag acgttgttcc ggagtggctg tacgtgactc
     6961 tggatctccg cactgtatca tgaaaccatt gatcacacga tggaacagga tgttgtcata
     7021 gtagtggcct gcagccagct taacaaaatt atcccggtgt tgtggtgtct cgctgtaaag
     7081 tctgatgatc atggaacctt ctgtggtctt gatttcaaaa acaggttctt cgggcagtgt
     7141 ggccggatca aaagcctgtt ccgtggcagc ttctgtggat atttcatttg ctgtcacagc
     7201 agccgcttct tttgcttttt tacccgtgtt gttacaggac cccatgaaga gtgcactcat
     7261 accgatcaga atgattaatg ttgatttttt catatagatg aacatgatta tttctaagat
     7321 ttccggaggg cgaaggtagc gttttttttt atatttgcat tcatgaaaat caagataatc
     7381 aaccattctc cctatcccct gcctgagtat gcaactcccc tctctgcagg gatggatctg
     7441 cgggcacata ttccgggagt cattaccttg cagccgctgg aaaggagtct ggttcccacc
     7501 ggtttgttca ttgaattgcc tgcgggctat gaagcacaga tcagaccgcg cagcggcatg
     7561 gccgcccgca agggaatagg cattctaaac agtccgggga ccatagatgc cgattaccgc
     7621 ggagaaattg gcatcatact ggtcaatttt tccggccagg ctgcggagat cgcgcccggt
     7681 gaacgaattg cacaactggt agtggcttca caccagaggg tggtatggga acccgtggag
     7741 caactggaag aaacagaaag gggaaaaggt ggttttggat ctaccggtca tcagtaatca
     7801 ttaacctatg aatcgtctgg tcgcactgtt tgaaaagcat cccgatatat gcaccgacac
     7861 ccggcaaatc cgtccgggtt gcctgttttt tgccctaaag ggagaacatt ttgacgggaa
     7921 catgtttgct tcccaggccc tggagaacgg agccgcctat gctgtggtgg atcaatacca
     7981 cggaaccgat ccgcgctgta ttttggtacc cgatgtgttg caggctttac agcaactggc
     8041 cgcagtgcat cgcagccggt ttgccggtcc ggtcctggcc ctgaccggat ccaacggaaa
     8101 aaccaccacc aaggaactca ttcacgccgt gctttctgta cgctataatg taacagccac
     8161 gcaagggaat ctgaacaacc atataggggt tccgctgagc cttcttaaga tgggccccgc
     8221 aacagaagcc ggactgtttg agatgggagc gagccatccg ggagaaatag cctttcttac
     8281 agacatggta aaacccaatg ccggcctgat aaccaacatt tccggggctc acctggaagg
     8341 atttgggaac ctggacgggg taaagcaatg taaaggggct ttgtatgatt acctgcatgc
     8401 ccatgaggga accattttcc ataacgcaga tgatcccgaa ttaacggaaa tgctggagca
     8461 aagaacgatc cgcgatcacc tcatacctta cggaataagc aaacagcccg tacagacctc
     8521 actggaagat ggtgtattgg tcatcacagt gtcgggttat ccgcaaatca gaacgcacct
     8581 ggccgggtta tacaacacac ccaatgtcct ggcagcactg gcagtagccc ggtattacgg
     8641 aattgaaaac acaaaggccg cagaagccgt tgccggctac attccttcca acaaccgttc
     8701 acagtggatc aataccgggc gcaatgctct catagccgat gcatacaatg ccaatcccgt
     8761 gagcatgtca ttggctttga aaaacctgga ttcgatccgg gaagggaaaa aatgcgccat
     8821 cctggcagat atgcttgagc tgggggctta tgcgcatcag ttccatctgg gcattgtaaa
     8881 gcaggtagaa cagatgggac ttgagcaggt gttctgggta ggaccgctat tttcaaaagc
     8941 cgtgcaggaa cttggggcgg cgggaatgtg tttttccaaa gtggaagacc tgatcctgca
     9001 cctggctacc agacccctgg aaggagctac ggttttggtc aaaggatcca acgggatggg
     9061 tctttcaaaa tttattgcat ccggagtttg ctgaaagtca cctttttcag ggaaaggaaa
     9121 aaccgcaaaa caatactgcc cctgtacacc acgcctgaaa agttccgggc gctctctttt
     9181 tttgtccgtt ccactgagcg gcgtattgcc cagaaatgac ggtgcgcttt ccacacagaa
     9241 ccggtaaaag accatttgcc ctggaacaga taggcaatgg ccgacagatg atctacaaac
     9301 agcctcaggg ctatgatggg tacaacctcg ccgttgtcaa ggtttttgta catcatgagc
     9361 aggttgttcc tgtaattcag atagagtttg tgcggggaat cgttgggcag ggtgcccccg
     9421 cccacgtgaa ggatctttga ttccggaacc acggcaatgg tataacctgc ccattgcgca
     9481 cgccagcaga aatcaatctc ttccatgtgg gcaaaaaaat ggtcatcaag acctttcagg
     9541 ctgtgaaaca aaacggaacg taccaccagg caagtgccac ttgcccagaa aatgtcggcc
     9601 ggcttatcat actgcccgtg atcggtttca atggaagtaa ggattcgtcc cctgcaaaag
     9661 ggaaacccaa ggcggtctat gaatcctccg caagcccccg cgtattcgaa cctgtcgggg
     9721 tttgtccagg aaagtatctt gggcatgcac actccgcaac cggtttggct gtccatatat
     9781 gtgaccaggg gctgtagcca tccgtgagta acctctacgt ctgagttgag cagtatgtag
     9841 tattctgctt caatttgttc caatgctttg ttgtacccgc ccgtatagcc caggttgcga
     9901 tcgaatgaga tcaggtgcat gccggggaag ttgtcccgga gccaggcgac tgaaccgtct
     9961 gttgaaccat tgtctgccac atatatgcgc gcccagggct cgggtgtacg tgtgaccagc
    10021 aattgcagga agcgttccag aaaatatttt ccgttccagt ttaggatcac tatggccact
    10081 ttcggcatca taagacaaat gtaagggttt ttcgtaaatt cgcttcatga aaccgaaggt
    10141 ggaattgctg gcacctgcca ggaacctgga atgtgccgtg gaagcggtca accatggagc
    10201 cgatgccgtt tacatggggg cagacaggtt cggagcccgt gcggcggcag gcaattcact
    10261 cagcgaaatc gggcaggcgg tcctttatgc gcatcgttac ggagcccgta tatatattac
    10321 catgaatacg ctgttgttcg acagcgaact tgaagaggcg gtccggatgg cccgcggact
    10381 ttatgacgcg ggggccgatg cgctgatcat ccaggacatc agcctgttgt acctgcgccg
    10441 gcacaatgtg tttcctccgt tgcccataca tgccagcacg caaatggaca atgcttctgc
    10501 ggaaaaagtg ctgtacctgg agaaggaagg gtttgacaga gtagtgctgg cccgggaact
    10561 ggctcctgaa caaatacgcc ggatcaggga aagaacatct gttgaactgg aatgctttgt
    10621 ccacggtgcg ttatgtgtga gctacagcgg caagtgctat atcagtcagg aagattgcgg
    10681 ccgcagtgcc aaccggggag aatgtgcgca gtattgccgt ctgtcctatg acctggaaga
    10741 tgcggcggga aatgtgcttc tcaggaacaa gcacctgctc tccctcaagg atatggatcg
    10801 ctcggaacgt ttacaggaat tgattgacgc gggagtgcat tcgttcaaga tcgaaggccg
    10861 cctgaaggat gtctcttacg tgaaaaacat cacggcctat tacaggcagc aactggaccg
    10921 gctgcagggg caaaaaacct cactgggaac atgtacgttc tttttcagac ccgatcccca
    10981 aaaaacattt caccgcgacc agaccggata tttccccgaa aacggtcgtt ttaccgagcc
    11041 tgtccatcag tggtacaccc caaaatccac cggcgaatac ctgggaaaaa tcaaccgtgt
    11101 ctttcccgga ggatttgaga ccaatgcggt ggtgaataca ggtgacggac tttgttttac
    11161 actccctgac ggtacctttt ccggtttttc cgtcaacagg gtggaaggaa aacgcatttt
    11221 ccgggaaggc actcccctgg aaaagggaat ggaaatatac aggaaccacg accggctgtt
    11281 cgaaaagcaa ctgagaggaa aaacggctga gcgccggatg gatatggata tagagatagc
    11341 agcccggaac gggggattat ttctttccct gatcagggaa tctctcccgg gggagccgcc
    11401 tgcccgggtt accgtaccga tagactgtcc ttatgatgag gctaaggacc cctctcaggc
    11461 cagggatttc ctgtcagcaa cggtctcccg gatgggagat actccgtaca ggatacgcaa
    11521 ggtgtttttt tcgcccgtgg actttccctg gttcgttcct gcctctgtac tggccctggc
    11581 caaaaaagag gccctggaaa gaatggaact actggtccag tcaaatgcac gtggttttca
    11641 tgttaccccg cacgaactgc ctgaacagga tcctttaacg gccttgcaaa agaattatcc
    11701 gcccggatca tacctgacag cggctacacc cattggagaa catacccccc tgatgacctg
    11761 ccggtattgc ctgaaacagg ccctgggata ttgtccgcga gaaaaaaaga atacaccggg
    11821 aaccttgcgg gaacctcttt tcctggtcag ggggcaaaaa cgttttcctg tctcctttga
    11881 ctgtcaggag tgtcttatgc tggtgggaag ggccgcatcg agcaagcgag ccagacccca
    11941 ggaaatgaaa aagtaaatga cagaaatcac gatcagggga aaaaaggcat caaaagtacg
    12001 actgcgtatc aaatcgctgg ctttggtcag atcctgcact gctatgtatc ctacaatgga
    12061 agtgagcttg gtaagagcaa tgagttcgcc tttgtatatg ggatacacct gcctgatagc
    12121 ttgaggaagg actatatgta tgaaggtgtt tatcttgctg aatcccaggg cgattcccgc
    12181 ttctgtctgc ccccggggaa cggattgaat ggcagaacgg aacatttccg acacgtaagc
    12241 cccgaagatc atggaaaaag tgattaccga tacagtaaca ccggtcattt ttgttgaagc
    12301 aaagaccacg taatacatca gcatcaaaag aacaacctgt ggaatgcccc tgaaaaatga
    12361 aatatagccg gaagccatca ggcgaaggat ctttctgcgg ctcatgcgca ggtaacaaat
    12421 gacggccccg gtcagggtgc ctcccagcgt agacaggaag gctatgagca gggtgatctt
    12481 caggccgacc cataaaagaa tatatcgttt ctccaggatg atattgttgt agaaacgttc
    12541 cttcaatccc tggcggggct tatcaaccgc cataccctgt tccagcctgg aacgcagcgt
    12601 tatcatggat gaataggttg taaggtaggt ttctgaaaag gaatatttaa gagtcctttc
    12661 cggtgttacc atgatgtaat tgaccatgat gtccatttta ccggccaaca tgcctgccag
    12721 catgccgcta aaatccgaga tcacaaagac cggttttctt cccatccagg cagcaaacct
    12781 ccggatcaat tctatatcga accctgccgg ttgattgttc tggtaaaaag agaagggaac
    12841 atccatacag gtggtgccta tccgtagcaa cttctgactt gttgtgcttt caggaaagtc
    12901 aggcatggtg ttgccggatt ctgccagcca ccgttcttcc atttgcctgt ggatgccatt
    12961 atcctgtagt tcttgcagga attcattaaa gcggggcaga aaaacagtgt cattcaacgg
    13021 gaacccggcc gagacggcgg taggagcgac ccgttcatcc aagaatgcca ggtccggggc
    13081 ctcttccagg atgtatatac agggcggttg tgccagaaag acaacatcac agcccccgct
    13141 ccttaacagg gaaagcaggt cggttgtatt ggcaagcggt aaaattcttg ctttagggaa
    13201 acgttctctt gcatagctct cctgccatgt ggctatgaga accccaatgc ggatatccgg
    13261 attatccagg tcctctattg ttttcaggga gcgttgtccg tacagggcac tggatccggc
    13321 cataacaaag agcagggcag caagcagtat tctcttcaac aggtttttca catcaggagt
    13381 tttaaatgga ttttggggca gacctgttag aaaaacggga taacagtacg cctgtaatta
    13441 tcaggatcag tcctatgacg gtagatggaa atatgggttc gcccagcaca tagtggataa
    13501 gaatgagtga aaggaagggg aacagataga tcagggaact taccacagcg gtgttttttg
    13561 ccagtttgag agctttgagc cagacaacaa acggaacggc catttcaaag atcccgatat
    13621 aaatccccga caggatactt ttcaggagag cgggtgaagc agggcccgtg ccgtttcgca
    13681 acgaaaccag cccgcttagc aataaaaaca gggcaatgta aacccaggca aagagaaaat
    13741 tcatgagcaa tgatatctca tcgggcaatg tgtttttcat attgatcaaa aaatacacgg
    13801 cccaaaggaa agcactgccc aaagccagta caacacccca gggatttttt atgagcagcc
    13861 cttcccggaa aacagtaagc gggtctccct gcagtgcaat caccagtgct cccgcaaaac
    13921 ttatgaccat ggccagtatg cctgcccagc ggatcttctg ccccaggatg ggaatggata
    13981 acaggaccag gatgagcggc cagatcatgt tcagtgcctg ggccacttgt gccggcagca
    14041 cgctgtaagc tttgaaaagg ataaggtaat agaggaatgg attcacaagt cccagaaagg
    14101 cactcctttt ccaggccttc cagggtattg caaaaaaatc gccacggtat cctcctgcac
    14161 gcaacaggat gaataacgtg gcgatagcag ccgtggtggc tatgaatagc atggatgcag
    14221 gggtcagtcc ttccagcgcc catttgaatg ccgtggccac agtagaccag catgctatgg
    14281 caatggcggc ataaaaaccg gctgacccgc gaagtttttt tgctattttg ctaccctttc
    14341 cagccattta accatagaca gcatagtaat catagatatg agacatacta caacaaatac
    14401 cgtccaggta gcccataagg gaatatgtga atacatcagg cctcctatga atagcaactg
    14461 gtttcctaca gcggtggcgg caagccatcc cccctgcatc aatccctgca tatgaggcgg
    14521 ggcgaccttg gacacgaatg acaatccgag cggactgatg aacagctcgg caatagtcaa
    14581 aacaaaatat gttgatatca ggataaaagg gcctactttg gcagattgct ccagccctcc
    14641 ggctgccttg acttcactga aaaggggaag gttgattgat gcaatggtca tgatcaggta
    14701 agcggcggcg gcaattccca taccaatggc gatctttttt ggtgtggaag gttccattcc
    14761 ttttttgttc agccaggaga agaacgggac gataagcagg gtcagtaaca ctacaaacaa
    14821 aggatttaca gattggaaaa tttctgcccc ttctatgcgt gtaaatccaa ggtcgatgtc
    14881 tatcatgctc aggtctgtgt agtcgcgcgc aaagaaagtc agagagaggc cgttctggtg
    14941 gaaagaaaac cagaagaaga tcacaaccgc gaacacggca aacagtgcaa gaagtcgttg
    15001 tttgacctcg gcaacatcca ttttggttac agtagaggcg gttttatctt ttgcagctcc
    15061 gtctttttta acatcaggca gtcttttttt ggtggttatg aatatcacta gagaaatgac
    15121 catggccaca atggctacga aaaatgcata atggaacccg gtgctgaata cgttcaggta
    15181 cctgtctgcg aaggcggcca ggtcggttat gggaccttgg gtggcctcgg tggctgcaag
    15241 ggcgaattta tccatggctt ccccggtcag ttctcctgca cggaactggt gacaaagggc
    15301 aggcagttca gcattgtaga acaggccgtg cgactgcagc caccagtttc ttaccgcaat
    15361 agcaaagaac ggggcaaaaa ttgctccaat attgatgaac atgtaaaaga tctgaaaccc
    15421 ggtgtcccgt ttgtctgcat atttggggtt gtcgtacatt tgtcctacaa cagcctgcag
    15481 gttacctttg aacaggccgt taccaaaagc tatggtaaac aggcccacgc aggtaatggt
    15541 caggtaaagg ggcaggttgg gaacgggtgt gggtgtgggc aaccctataa gcaggtatcc
    15601 aacggccatc atgatgatac cggtaaggat ggttcccttg tagttttttg ttttatcggc
    15661 aatgacacct ccgacaagag ctaaaatata aatgagtgca tagaaaatgg agtaaataag
    15721 tccggtcgta gtttcgctca ggccaaattt tgctgataaa aacagagaca ggatggccat
    15781 catggtgtag aaaccgaagc gttcgcccat gttggccagc gcagcggcta tcaatccttt
    15841 gggttgtcct ttgaacatag taggtggtta tttttggttt atacccggca aaaatacgat
    15901 aaatttggta actttgtctg cctatgaagg cactattttc gcagcttgga tattttgccc
    15961 tttacacgat tgttttcctg ttaagtctgc ttccactgcc tgtcctctat ggcctttccc
    16021 gggttctggt ggtgttgtcc gtgaaccttg cgggttatcg caggaccgtt gtaatttcca
    16081 acctgaccag gtcctttcct gaaaaaacaa aacgggagat acttatcatt gcgcgggatt
    16141 tttaccggta cctgggacga ttgtgtgcag aaactatctg gctctttacc gcctcgcaac
    16201 aaagtgttat gaagcgtttt tccctggaag gctgtgaagt gccggaatca ttgaaaagaa
    16261 tgggtaaaag tgtggtcgtg atgcttccgc acatcgggaa ctgggagtac ctcagggcat
    16321 gtagttgtcc tggctttgga agtgtctgcg gatttgacca ggatcaggtg catatggtct
    16381 atcagcaggt aaactcaccc ctgtttgata aactgatgta cacccttcgc aacaggtaca
    16441 agacgggatg gcatcttctg gaaagcagga gagcaggctc gcatatggca aggcacaggg
    16501 atgtccaggg tatctatacc atgatagccg atcagtgccc gggcccggca gggaaaagtt
    16561 atgccgtaag tttcatggga caacccacac tgatgatagc aggacccgaa accttggcgc
    16621 gaaaattcaa ttttgcggtg gtgtacggag tgatccgacc atcggcaaag ggacattata
    16681 tgcttcattt taccctgata agcgagaatg cggccgtgca tgaagaaggt tatgtgaccc
    16741 gccgttttgc ccagctgctg gagcaggata tcaggaaaaa tccccacctt tggctttgga
    16801 gccataaacg ctggaaaagg acaccggggc aaggctcgat atttgcataa aacaaataaa
    16861 attaaccgtt atgaaacagc tgatcattct attactgacc ataagcctat gggtgccctt
    16921 gcagggacag cccgtaacgg aagggcagca gatccccccg ggggctgttg tctacagcct
    16981 gccccgcaca tgcgtgcgta ttgtggccga ggctgtctat gtcaatttca accccggacc
    17041 ttatgcgcgt tatgcaagga aattccttgg catagacgcc ggccaggcaa aaaccaccac
    17101 ctatgaactt tcgcgcgtgg aaatgacacc catgctggag gccgaccccc accagcttta
    17161 tgtggtcaac ctgtctactg ccaatgccca gccgtggttc cttcaactga catcccaggg
    17221 tctggttctt ttatccgatg cctacgcatc gaaagagaac aactggagat tcccttcgct
    17281 ggacgtgtcg ccggatgttt cagatgcagg gatcattgaa aacctgaaag aagaacggac
    17341 taccctttac cgttcacagg taaggggtcg cgaagtgtct gccatccccg tgcaacagac
    17401 acaactggtt gaaaaaacac tggaacagag agccgaagag gccgccagaa agattttcga
    17461 gctgcgcaat atgcgttttg ccatcatcac gggggacacc gatgctgttt acagcgggga
    17521 ggccatggga gctgcggtgg aagagatgcg gcgcatggaa gaagagtacc tgaaattgtt
    17581 tctaggtgaa actactacag gaaaaacaca tctttccaca gatgtgctgc ccgatccgga
    17641 taatacacgc tatgtggcct tccgtttctc ggagactgag ggattgctgc cttccggcaa
    17701 tgtgggtggc cggcctatcc tgctggaaat agacaccagt gcggaagaga ccataccccg
    17761 gctcctggca gatgaaagcc gtatcagaac cggtttgcgc ctgtactaca ggcagccggt
    17821 cataaccctg gtgcggctta ccgacggtca gaaagaactg ctccaggccc gtattcccgt
    17881 ataccagaaa ggaccagtac tgaattttat cattaattaa aaaaaacatt tatggacatt
    17941 atcaacctca aaccccaaag agtctggaag catttttatt ctctgacgca aattccgcgt
    18001 ccctcaaaaa aagaagaaaa aatccgtgct ttcctggctg aatttggcag gaacctggga
    18061 ctggaaacca ttgtggatga gaccgggaac gtgatcatac gtaagccggc cacccccggt
    18121 atggaaaacc acaagggtgt catccttcag gcacacatgg acatggtgcc gcaaaaaaac
    18181 aatgctacag aacacgattt cgaaaaggat cccattattc ccagaattga aggtgaatgg
    18241 gtatatgcaa ccgggacgac actgggtgcc gacaacggca tgggcatagc cgcagccatg
    18301 gctgtcctgg aggcccgcga tctgaagcat ggccctgtgg aagtgctgat cacaacagat
    18361 gaagaaacgg gaatgaccgg tgccagggga ctcaaaccgg gcgtattgca gggtgacatt
    18421 ctgctgaacc tggactcgga ggatgagggt gagctttacg tgggatgtgc cggaggcgaa
    18481 gacggctcat ttgaaatgga gtaccagcct tctcctgttc ctgccggatg gaaagctgcc
    18541 cgcatcaatg ttacggggct caagggagga cactcgggaa tggatatcat actggggcgg
    18601 gggaatgcca acaagatcat gacacggatc ctgtaccatc tgttgcagga atgcaacatc
    18661 tgcattgcct gggctgaagg cgggaatctg cgcaatgcga tcccgcggga ggcagatgcc
    18721 gtgatatgct ttccttccga cgatgcagct tgcgtcaaag gcaaggttga aaacatggcc
    18781 ggaatcattg gaaatgaact taaacaggtg gatccgggac tggaagtgaa tgttaccatg
    18841 tgtgacatcc ctgcccatgt gatcccgtcg gaaattgccc ttaagatcat ccgtgcagta
    18901 tacgctgctc ccaacggggt gtgggggatg agcgcatcca tgccgggact ggtggagaca
    18961 tcgtccaacc tggctatttt caaggcaaaa gacgggaaaa tggccgttca ttgcctgatc
    19021 cgcagcctgg cagatacagc aaaacaggat atgggaacgg ccttggcctg ctgctttgcg
    19081 aacattggtg ccagtgtttc attctccgga gcttatccgg gctggaggcc cgatatggat
    19141 tcccccatcc tgaaagtgat gctggataca tacaaaaaga tgtacaaaca ggaagcaaaa
    19201 gtcaaagcta tccatgcagg actggaatgt ggcatactgg gtggaaccta tccgcactgg
    19261 gatatgatct cgtttggacc cacattgcgt tcaccgcact cacccgaaga acgctgcctg
    19321 ataccgactg tccagaaatt ctgggacctg atagttgaaa cattgcaaaa catcccggaa
    19381 aaacagtaag cctgtttctc taataaataa aaaatttgca gggctgcagg aatttcctat
    19441 ctttgcagcc ctttataatt ttataataat aaccaatcac tatgttgcaa cagtacgaaa
    19501 ccgttttcat tttaactccc gttttgtctg acgcccagat gaaggaaacg gtaaccaagt
    19561 ttcgtgatac gatccttgcc aacgaaggtg agattgttta cgaagaggat tgggggctga
    19621 aaaagctggc ttaccccatc cagaaaaaaa ccaccggttt ttaccatttg ataggattca
    19681 aggcaaatcc cgatttcatt gccaggctgg aactccagtt tcgcagggat gagcgtgtga
    19741 tccggtactt gacctttgca atggacaaac atgccgttgc ctatgcagag aaaaaacacg
    19801 gccaggtcaa agatgccgcc cctgtagtgg aaaaggaaga aaaacctgcc cccagggtaa
    19861 cgaagccggt tgtggatgat gatattgaaa ttgacgagga ggactagaac atggcacaaa
    19921 acaatgagat tcgttttctg aaccccccta cagttgaggt aaaaaagaaa aaatactgtc
    19981 gtttcaaaaa agcagggatc aaatatatcg actataaaga ccccgagttc ctgaaacgat
    20041 ttctgaatga acagggcaag atcctgcccc gcaggcttac cggaacatct ttgaaatacc
    20101 agcgtaaagt ggcccaggcc gttaaacgtg cccgccacct tgctttgctg ccctatgtca
    20161 ccgatttact taaataagga gagaggctat ggaaatcata ttaaaacaag atgttgaaaa
    20221 actgggacat aaggatgaca ttgtgaaagt ccgcaatgga tatgccacca actaccttat
    20281 tcctaaaggg atggccgttc tggcaacgcc ttctgcagtg aaaatacacc aggaaaatat
    20341 gcgtcagcgt gcccataagg aaaccaggat tcgtgaagac gcccaggccc ttgccgccaa
    20401 actggcagca tgccaggtca ggatcgccac caaagtaagc agcaacggca aagtattcgg
    20461 atcggtgaac aacatccagg tagctgaagc ccttgcagcc cagggaattg aagtggaccg
    20521 ccggaacatc actttcaccg gtgcagaatc cttgaaggaa gtgggtgtac atgaaacagt
    20581 catcaagatt tatcgtgata tcatcgccac cattcaggta gaagttgttg gcgaggaatg
    20641 aatcccttct tgaaatgagt aagaacccgg tgagcccggg tttttttcgt atctttacgg
    20701 actaattttt ttgaaatgaa acaggatata accctatttg atcttatcag gaaagaggag
    20761 aaccgccaga ccaacggcat tgaattgatt gcttccgaga attttgtcag cccccaggtg
    20821 ttaagtgcca tgggatctgt actgaccaac aaatatgcag agggataccc cggcgcacgt
    20881 tattacggag gttgtgaagt ggttgacaaa tcggaacagc tttgcataga caggatctgt
    20941 gaattgtttg gtgccgagta tgccaatgtg cagccccatt cgggcgcaca ggccaatatg
    21001 gctgttttcc ttgcatgcct gcaaccggga gataccttca tgggtcttga cctggcacac
    21061 ggaggtcacc tgacacacgg ctcgcccgtc aatttttcag ggattctgta ccgtcccgtt
    21121 gcctatacac tgaaccctga aacaggtgtt gtagattatg atcagatgga acgcctggca
    21181 ctggaacata aacccaggat gatcataggc ggcgggtctg cctatccaag ggaatgggat
    21241 tatgagcgca tgcgcgggat cgcagacaag gtcggagcca ttttcatggt ggacatggcc
    21301 catcccgcag gactgatcgc tgccggactg cttgataacc ccgtgaaata tgctcatatt
    21361 gtcacttcaa ccacccacaa gacactcagg ggtcccaggg gcggcatcat tcttataggt
    21421 aaggattttg ataatccgtg gggcatcacc acacccaagg gcgttgtaag gaaaatgtcc
    21481 tccctgatca attccagtgt gttccccgga gtgcagggcg gtccgctgga acacatcatt
    21541 gcagccaagg cagtgtcgtt ctttgaagcg ttgcagcccg agttcaggga ttaccaggtt
    21601 caggtgcgga aaaacgcaca ggcattggcc tcggctttta tcaggagaaa ctacacactc
    21661 gtatcgggtg gcacagataa ccatttgctg ctgatagacc tgcggtcaaa atttcctgaa
    21721 ctgacaggaa aagaggcaga aaatgtgctg gtcagggcag acattaccgt gaacaagaac
    21781 atggtgcctt acgattcccg ttctcctttc aagacctcag gaattcgcgt gggaacgcct
    21841 gccgtgacca cacgcggtct taaggaactg gacatggaga ccattgtggc gcttgttgat
    21901 gaagtgttga gcaacctgga tgatcaggca gtcattgacc gggtaaaaag aaaagtgaaa
    21961 gaactgatgg gcgggctccc cctgtacgcc tggtaaatat aatttttatg aaaagaaata
    22021 cactgactat tgccctactg attgctgtgg ctttactatt atgcgaaaca tcaggagcac
    22081 aagttcaaaa aaggggttat actgaagcag tcataggtac tgatatcaat gacatacttc
    22141 cggacgatat gatattcatg tatccggaat ttctgaaagg cagcgtgcat ttcctggaca
    22201 ggagcgtagc cgagggagtg atgaatatct atttgcccgg attgcaactt tactttattg
    22261 actccaataa ggatacgctg gtgatgagaa atcaggacga ggcccggttg ctcacgattg
    22321 gccatgatac ttacctgaga catgacaaaa catgggtaag gatcctggct acaagcggaa
    22381 cagctgcctt ctgcctgaaa tcagttttga aaattcacca ggatgtgaaa atcggagctt
    22441 atggaacaac tgatgtcaca tcgtcaatca ccaacgtcga tatgatgtat ggtgtggaag
    22501 gtaatactgc tatcaggttg aagagcctga ggcatgtccc gtatactctt acccattttg
    22561 gcttactgta tgatggcagc aaattgttta ttgccaacgt ccggaatttc aaaagaatgt
    22621 ttcccgacag aaaagacgat attgacaaat atatcgctga aaacaagctg aacctgaatg
    22681 acgcaaagaa tgcgttacaa ttattcaatt tcctgaatct gtagctgcct gcgctcctga
    22741 acaggtctta ttctcctggt ctgagccgga atggtatccc gttttgccgg aatgttgtcc
    22801 tgctgtgcag gagctgccgg tatttcctta agcggaatgt aaggattgac cttgttttcc
    22861 ctttccctct gactgagttc cttcacggca tctgtggtta caccggtacg cagtgttgaa
    22921 ggcaaggcgt ggacaaatgg aaaatattta agggtatagg ggaacaaggg ttctttataa
    22981 atgatccctg gttctgaaaa ggtctcctct atagtacgtg aagttatgga agtgcggtcc
    23041 agtggccttt cagcgcctct ccagtcgaaa cccgatagcc gctcctgttc tttggtcagg
    23101 tcatacaggg gtatcagatc gctctttacc gattttttat aggttatgcg ctgagcccgc
    23161 ctgtctttca ggatgacttt catttcttca cattctttaa tgttcaggga agtgatgatg
    23221 ctgtcttcgg gaatgtagaa aagagcccta gtgtttttca gggcgtcgaa ccggttgaca
    23281 tcattatcgc tgatgtgagc gtacatgagg tctgccttga tctggtggaa gaacacctgt
    23341 tcttcctggg aaatgaggaa tgcggcggaa agaaaatcaa cccggtagag ggaatccctg
    23401 ctgaaccgga actggatgct gtctgcactt aactggcttt cctcattcca cagtacaggc
    23461 tttccatatt gccgcacaac tgaatctatg ctgttgaata ccaaggagtc gcatatagac
    23521 tgtatgtcac ttttgtacac cctgacattg ttgtaaccgt ataaaaagtg cacgtcagta
    23581 gtattgaaca gactgtcacc caaaatcttt acatcggcaa ggagggtgct gtccgcccgg
    23641 accaggaaca cctgcacatc acgcagattt tgcctgaggt aatttacggc cgcttctgcc
    23701 gtgggatccg ggatggtatc cctgccggac ggcagggagt cctttgcagg cgggagggag
    23761 tccttttcgg gcagggcagg ggatcctggc aggtttacag aaccgggcat tgcatgcgaa
    23821 gaggaatcgg gaagtatgat ggaatcaggg gcagctgccg gtctcttccg ttcaggaatc
    23881 tcctgccttt caaaggcacg cacacgctcc agggagtcca ttttcccata tgtggatgta
    23941 taggcgagca gggtgtcggc acgaaggaat agcgaatccc tgcggttcgt ttcactgtca
    24001 atctcccaga cgaccaaaac gggatcttcc gttaccctga cccaatccgg ttctctccgg
    24061 aaatccagcc tgtcgcccag gatgtggatc tgctgggtcg tgtccctgac ctgtacatta
    24121 ttgcgcaata tgccggaatt gcttcccttg ttgtacagca catcttcggc cctgatttcc
    24181 tgttcgtccg agcggatgaa gacatcgtcc aggaaatggt acaattcttc atctctgtga
    24241 taggtgcctt cccgggaggt gaggaaacca tcggtatgcc aggcctgtac atttccccag
    24301 aattctgcca aattcgaatc ggaatgataa tacagggaat cggcttttat cctgagtgtg
    24361 tcggtgccca tttccacgtg ctcatacagg cagaaaagtt tttcctttga ataataataa
    24421 cccctgcggc tttccagtat ggttccttcg ttgtcgacca cgctgccacc attgaaaaaa
    24481 taggctatgc tatctttcgt gttgaaattc aggtattgag tacgcaaccg gttttcttcc
    24541 ttatctatga gttcaaccac acgaccccgg acttctgccg tgttgaggtc ccccaggtaa
    24601 tgaatggttt ctcctgtcag ccgcgtgcct tcctggatga tttgaatatt tcccacagca
    24661 tctattatgt tttctgctgt attccagata gctgtgtcac agagaatata cgtgttattg
    24721 tgaaggaaac gggcaggacc agtgattttc cggtaactga caccaaaaag ttcatacatc
    24781 tcggccatct gggcactgat aaggtgtact ttgtcttcct ctgtcctttc ctgtcttatg
    24841 gtcaggactg tattgctccg ggctgtgatc aacccggcca aaaaaaggaa aagtagcaga
    24901 acccggtgtt ttttcattat ttgatccagc cttcccgctt gaagggacaa tccctgaaca
    24961 gggggtcaat acggatgtat gtggtctgtt gtttttcttt gatgaatctt tctacggcat
    25021 tctggctttc cttctcttta gacatattct gaatgatgta gtaatcatcc tggagattgg
    25081 ccacatggga gggaacgatc ttgtccagac ggattatctt atagatgaca ttcccatcac
    25141 gtccttcatt gtcccggctt tcaaaagcaa aggaaatatc gccttccttc atgtctttga
    25201 gcacattata atcatttgtc ttgagctggt ctttgtcaaa gagcatggac ccggagtttt
    25261 cgtccgacat acgtccccca ttaagacggg aaggcggatc tccggaatat ttcatggcag
    25321 ccttttcaaa tgtgagactg tccgatttga tggctgtggc aatactgtcc aggcgtccca
    25381 ggcatttcag cttgtctgca gtaagcacgt cgggtttgag gagtatatgg cgtgcattga
    25441 acatatctcc ttcgcgttca atcatctgga tcagatggaa tccgtcaggg gtttccacaa
    25501 tttgtgatac ctgtccttct ttcaggacaa tggctgcatc accaaaagca ggccagtaca
    25561 tttgcttggc cgccatgccc agttctcctc cccgcatggc cgaataagga tcctgtgaat
    25621 agagtgtggc cagtgtgttg aaacgttccc cattgataat tctttcacgg aactcgagaa
    25681 gacgctcctt gaccagcagg atggcttcct ctttgggcgg gtatagaact atctggctga
    25741 gctggtattg ggtgggaatt atgggcatgc tgtcttttga ggtacggtcg taaaaatcct
    25801 cgatatcctt gggtgtcatg tcggggattt tatcggttat ggcatattgc atgtcacgtg
    25861 tgaggctctg ctcgcggaac aggtcccgcc attcctgttt cagtttgaaa atcggtttgt
    25921 gaaaatagtc ttccgttgct ttttctccgc ccagacgtgt cttgatctcc tgaagcctgt
    25981 tctccagttc cagctccaca tattcttcat tgactgtcag gctgtctaag cgggcttgat
    26041 taagcatgag cttggatacc agcatgttct ccaatatttc gcagcgcagg ttcttgtcgg
    26101 tgatgattcc ctgcataatt tgcatctgca cttcttcttc tatggctgaa agctggatca
    26161 tttcgttccc gacaagtgcg ataaccttat ctaccaggcc gttggcgtat ttttgaccaa
    26221 atgcgaaagt cgttattgat aggaaaaaaa gcaggcttga agcaatttta ttaatcttca
    26281 ttttttctgt agatttttat catttgtttg ttccagccct ctttcaacac ctcaacttca
    26341 aggtcttcca gcaatttctg ttttctttta attatgatga tctggcgtat tcttgcttct
    26401 gtctgttcta tgggtgcagg ttcaccggcc ggaacaaagt catataccct tacaaggtag
    26461 gcatacaggc tgtcacgcgt atctatgtat ccgtctgctt tcaatgcctg tatcttgtcc
    26521 atgttgccaa ccggcaaatc ctgtgccaat tcctgaccgg taagccactt gccttcataa
    26581 tccgtgtcga tttctgcaga gctttcgcac aaacgtctca attgcgtctg gtcttcaatg
    26641 gtgttggatc ggtaaatatt gcgcaccatg ggaaggtaag gcgagtggag cctcattttt
    26701 atcaacctga gcttgagaag aggatcgtcc agcaggaata gttcaagatt gtcttggtaa
    26761 taggccaaga tctcttcttg ttctatgacc gtgtcgatac gttcttccac gtattttttc
    26821 tggtacctga agaccagcag ggatttcctg taatcggcca attcctggtc cacatccttg
    26881 tcttctttgg atagatcagc ctcggccttt ttcagcaaaa ggttatcgat ggcccaaagg
    26941 tcaatatagt gattgacaag gttcagagaa tcatcagaag tggcggcgct tttcaacaat
    27001 ccctcaacct cgtcaatgta gagaatctct tttccaatct gggccaccct ttccggttcg
    27061 ttccccaacc atcgtctgat ctggtggcag gaagtcagcg ttgtcaaaag gagtagtatg
    27121 aggatccact ttttcatgac cgtttttatc tggaataatt gggagcttca cgggtaatag
    27181 tcacgtcgtg cggatggttt tctatgacac cggcagatgt gatgcgtacg aattctgctt
    27241 ttttcagtga tgccaggtcc ggtgtgccgc agtatcccat tccggcacga agtcctcccg
    27301 ccagctggta aaagacttcg ctcaatgttc ccttgtaggg aacctgggcg acaatgcctt
    27361 cggggaccag ttttttcaca tcttcctcgg catcctggaa ataacggtct ttggaccctt
    27421 gttgcatggc ttccagggat cccatgccac ggtagctctt gaattttctt ccgttcagga
    27481 tgatggtctc acccggagac tcttccactc cggcgaaaag cgatccggcc ataacggtat
    27541 cggcccctgc cgccaaagcc ttgaccatat cccccgaata ccgcacgccg ccgtcggcaa
    27601 ttacaggaac acccttgtcc ttaagcacgc tataagctcc catgattgcc gataattgcg
    27661 gaactcctac acctgcaacc acgcgtgtgg tacagataga accggggcca atgccaacct
    27721 tgacagcatc tgccccgcat tcggcaaggg cgagtgcgcc ggtagcagtg gccacattac
    27781 cggctacaat atcaaggtga ggaaatgctt tttttgcttt acgcaacgtt tccagaacgc
    27841 tgattgaatg accgtgcgct gtgtcaatga ctaccgcatc aatatctact gacaggagtt
    27901 tttctatgtg ttccagggta ttggcccgta ctccaacagc tgctgccgta cgaagccgtc
    27961 ctttgctgtc tttggttgcc ctgggattat ccttgatttt tcttatatcc ctgaatgtca
    28021 gcagtcctat aagtttataa ttctcgtcta ctaccggtag tttctcaatc ttgtacttct
    28081 tgagcaattc tgtggcagtg ctcagatcgg ccccgttacg gatggtaatc aggttttcct
    28141 ttgtcatgac ttctgtgatg ggagtggagt cattgttcac aaaacggagg tcacgattgg
    28201 tcactatgcc aataagccta tgcttactgt caaccaccgg tattcctcca atgctgttct
    28261 cggccatcag ccggagtgca tcacctacgg tagcttccgg tcctatgaca atgggatcat
    28321 agatcatccc gctctcggat cgctttactt tcctcacatg ttccatttgt tgttcaatgg
    28381 tcatgttctt atggatcacc cccaggccgc cttcacgcgc gatggctatg gcaagggtgg
    28441 catctgtcac agtatccatc gcagatgaca ctataggggt actgagtatg atattccttg
    28501 aaaaacgtgt agtcaggtcc acttcccggg gaagaacagc agaggcggca ggcaggagca
    28561 gcacatcgtc aaaggtgagc ccttcataga gtatccgatc acttattgat ttcatggcct
    28621 aagattttat cctgcaaaga taaagaaaac gactgattat gtattatctt tgtaaccatg
    28681 aagaaatgga agatcggctt acttgcccgg gttgccatag ccatcatact cggaattctg
    28741 tcgggattgt tcttcccaac ctgggcgtta cggatctttt tgacattcaa cggtcttttt
    28801 agcaacttcc ttgattttat tgtcccgctc attatagtgg gcctcattgc cccgggaatc
    28861 gcagaattgg gaaagggtgc cggtaaaatg ctgggcacaa ccatggcact ggcgtacggt
    28921 tccactgtat tgaccggttt ttttacctta ttagcctgta aattggtata tccaatcttg
    28981 ctggatggag cagaaactgc catgaccatg gcatctccgg cagaaaacgg attgtctgcc
    29041 ggagagcagc ttgtaccgta tttcaccctg gatttttccc ccctgtttgg agtgatgccg
    29101 gcactggttc ttgcctttgt actgggtctg ggactgacca tcatcaacaa cggggatgtc
    29161 ctgaaacagg cactcctggg attccgtgaa attgtgaaca agaccatatc aggggtgatt
    29221 attcccttat tgcctctctt tattttcggc atttttatga aaatggcagc cgaaggccat
    29281 gtcgtccagg taatgggagt gttcgttaag attgtaatat tgattttcat cctgaccgtt
    29341 atcctcctgg tgtttcaatt ttcggttgcc ggcctggtag caagaagaaa tccttttacc
    29401 atgctgcgca ccatgcttac cgcctacgtg acggcactgg ggacgcagtc ctctgcggca
    29461 accattccgg tgacattgca ccagaccatc agtttaggca tagatccgcg catagcagga
    29521 tctgtcatcc cgttgtgtgc aacggtgcac ctgtccggga gcatgctaaa gataaccgcc
    29581 tgtgccctgg cggtcatgat tatgatgaac ataccggttg acccggctgt ctttgtggga
    29641 tttattttcc tgctggctat tacgatgata gcagcacccg gtgttcccgg gggggccatc
    29701 atggctgcta taggggtact tcagtctgtt ctgggttttg accccgaggc aacaggattg
    29761 atgatatcac tgtatattgc gatggatagt tttggaacgg cgtgcaatgt aaccggtgac
    29821 ggggccattg ccgtcatagt ggataagatg tatgcgtagg gtaagtgtct tttgcggctc
    29881 ccatacgggg aacgacccgg gaataataca gcaggcagaa ctcctggggt ccatcctggc
    29941 agagcaagga atcgaacttg tttacggggg aacaaacatt ggcatcatgg gcgttctggc
    30001 caacagtgta ctggcctgtg ggggcagcgt cgagggggtg atgccttcgc tgatcgaaga
    30061 aaaacgcata gcccatcatc acctgacccg gatggtattg gtttcaacac tggaggaacg
    30121 caagaaaaag ctcatagaag actgcgatgc catagtggtt tttcccggga gttacggaac
    30181 gttggatgaa ttgttctcgg ctttggtcct gaagcagctg ggaaaaatcg acaagcccat
    30241 tgtgatgctc aattttcgcg gctattatga tcctttgctg gcccagctgg atcatatggt
    30301 aaaagcggga ttcatgccgg agcgttttcg ttcaaacctg caggcggttg aggattgcca
    30361 tcagttgcac tttctgtggg attgacgtac agggcgccct ggaaggtatt gcgttcttcc
    30421 agcctttttt caaggagacg caggatttcc acattgcgga tcagtcccca gggattggaa
    30481 tgcacaaaac ggggcggcac ttctccggcc acacgttcaa tgaatatatc ggggcgcagc
    30541 ctttccagaa aatctatgat aaagtccagg tactgatgga gtgaaaaagt gacaaagtct
    30601 ccgggttttt ccgcaaattc cttttccatg ggagttcctt tcacaatttg caactggtgg
    30661 agtttgaggc ttgtcagagg caaccggcta aggattccgg cttccgaaag catctcttcc
    30721 ctgctttctc ccggtaaccc gaatatcata tgaacacagg tgtgaatccc cagggcagcg
    30781 gtgtcacgga ttgcagcggc agattgttcc caggtgtggc aacggttgat gcgctgcagt
    30841 gtcctgttat agcaggattc tacgccgtat tccagaataa cgattttttc aagtgacagg
    30901 tccctgaaat acttcagctt tcgtgcatca atacaatcgg gacgggtagc caccacaatt
    30961 ccgctgatgc ggggatcttc cagggcaggc ttgtatatcc tgcgcaggtc ctccaatggg
    31021 gcatgtgtgt tggaataagg ctggaaataa gcaagataac ctcccgcttt acagtagcgc
    31081 acacgatgga attcaatgcc ctcatctatc tgcagggtca ggcttttatc cggtgtgctg
    31141 tagccgggat gaaaagcccg gttgtcgcaa taggtacaac ctcctgtacc tgtggtaccg
    31201 tcacggttgg gacaggaaaa accggcatca acgactactt tctgaaggcg ggttccgtac
    31261 cggcttatga aataacccgt aaaggatttg taacgttgca tcatgcaaag gtaacaagta
    31321 tgccttgtca aatcaaagat ttgccgtaaa tttgtaagat atgaaaaagc aattctatta
    31381 tcttgtcgca atttcagctg ctctggccgt actcgccgga tgcacgaaaa caaggacctt
    31441 ggaaaaactt tccaacattt ccgacaatgt gaaaactgct ttcactccgg acaggcgcga
    31501 taatgtttat gagatcgtgt tcgtaaaact aaagggttca aaaacttatg ttatgaaagg
    31561 ctctaccacc gaacccggcg tggtacccgc cctggttgct gccgctgcat cacaaggaat
    31621 agagcttaca gacagcattg tcttgttgcc cgatcctgcc ctggaaggcc gtatatatgg
    31681 tgttaccagc ctgtcagtgg ccaacctgcg ctatgaacca cgctattcat ccgagtcagc
    31741 cacgcagaca ctcatgggaa tgcccttgcg cattctggac aagaaaggaa gctggaccag
    31801 ggtcaggaca ccggaaggtt atatagcctg ggtttcttcg tccagcctgc aggagatgga
    31861 tgaacaaaca tacggtcaat ggatgaaagc acccaaagtc attgtaaata aacattatac
    31921 cttattcagg gagaagcctc ttccgggagc acctgtggtg tctgacgggg tgtggggcaa
    31981 tattgtggag ctggaagggc aaacaccttt gttttataag gtaaggctac cggatggaaa
    32041 acaggcatac ctgccacgct atgatgcaga accctttgag gcatgggcca gatcgggaaa
    32101 tcccctgccg gaagatatca ttgcaacggc catgcagttt gtgggcttcc cttatctttg
    32161 ggccggaact tccatcaagg caatggattg cagcggtctt gtaaaaaccg cattttacct
    32221 gaacgggatt gtcctgttgc gggatgcatc gcagcaggca cgcatgggag aacaggttga
    32281 cataagcgaa ggctggcaac gactgaccat gggtgacctg atatttttcg ggaggaaagc
    32341 cacggaggac aggaaggaaa gcattaccca cgtaggaata tatattggag acggccgttt
    32401 tattcattca gccgggaggg tcaggatcaa ctcgctggat cctgccagtg acatttatta
    32461 tgaaggttca gacaggttgc tgagggcagc caggatcctg aatcatattg atgacggaac
    32521 ccaagtggtt tctgtcaggg aacatccctg gtacgggatc aagactcaat aatgtaccat
    32581 aaccttgact aaacaatgaa ttcaacaagg cgcgattttc tgaaaacatc catggccatg
    32641 gccgcagcgg ctgctcttgc cagcccctcc agactgtttg ccggaaatgc aaaaaagaca
    32701 ctccaatcaa aaaaagccgc ctcatcggat aagatggtac ttaccttcga gcctttcgag
    32761 gcaagaatga aacatgtttt taccatttcg ggttcctccc gttccactac gcccatagtc
    32821 ctgaccagga tcacgtacca gggacatgtg ggctacgggg aagcctctat gccgccctat
    32881 ctgggagaaa gtaccgcgtc tgtgatggaa tttctcagtc gagtagatct gagccgtttc
    32941 agttcacctt tccagttgga agagatattg acctatgtgg acaatataac gaccaacaat
    33001 actgcagcca agtgtgctgt ggacattgcc ctgcacgatt tgtgcggaaa acttatggga
    33061 cagccctggt ggaagatctg gggatggagc aacaaaaacc ttcccagcac ctgcgtaaca
    33121 gtgggactgg cctatgcgcc ggatggaagc attgacaggg aagaggtgcg caaaaagaca
    33181 ctggaaaccc ttgattacaa cctgatcaag gtaaaactcg gcaacacgga agaaaatgat
    33241 aaaaccatga taaacaccat cagagaggta accgaccttc ctattgtggt ggatgccaac
    33301 cagggatgga aagagaaagg atatgccctt tccatgatgg aatggctggc tgcccgcggt
    33361 gtggaaatgg ttgaacaacc catgcccaag ctcctgctgg atgaagcggc ctggctgcgt
    33421 gaccggagtc cccttcccat tatggcagat gaagcctgcc aacgactgtc cgatgtacga
    33481 aaactttacg gtgcctacga cgggatcaat atcaaactga tgaaatgcac cggcatgcgt
    33541 gaagcccggg agatgataac ccttgccaaa gccctcaata tgcgaatcat gatgggctgc
    33601 atgacagaaa cctctgtagc catatcggca gctgctcaat tcgcaagtca gctggaatgg
    33661 gcagatctgg atggaaactg gttgattgcc aacgatatct tcaccggaat gaaagtcgtg
    33721 gacgggtaca taaccctgga agataaaccc gggatagggg ttgaaccatt ataaacaata
    33781 cataccaaag catatgcctg aaaatttaag aacacttaaa aaagattttg actatttgat
    33841 agatgaggtg atctcggatt gctgtacgtt cgtctatgtc aatcccgata aaaaagaggc
    33901 agaggccatg gacgttataa acgaggccat tgcgctcagg gatgagatgt ttgaacgact
    33961 gaacaagata cgcgagaaac cggcaaaacc ccatatcaaa gcgttgaagg aagaatttct
    34021 gaccaggata gatgccttat ttgaaaaaat aagtgaattg gcaaaataac ccgcccggga
    34081 gtcttttgtc agggcgcata ttgatcctgg acggagcaat ggggacaatg atccagcgtt
    34141 ttgctccgtc ttttcccccc ggaaacgatg ggaacaacga tatccttacc ctgacacagc
    34201 cggagctcat attgtccatt cacagggaat acgtacaggc cggcgccgat atcatcacca
    34261 ccaatacatt caacgcaaat gccatatccc agcaggatta cggcatgcag gaccaggttt
    34321 acacaatgaa ctttcaggcc gcacgcctgg caagagaaag tgatgcacgg tttgttgccg
    34381 gatccctggg cccgacgaac aaatcggcct ccatatcacc cgatatgaac aggcccgggg
    34441 tgcgtgccac ggattttgcc accctggtag gagcttaccg tgaacaggcc cgaggcctta
    34501 aagagggcgg tgtcgacctc ttcctggtag aaaccgtttt tgatgcactc aatgcaaagg
    34561 cggccctttt tgccctgtcc gaagaagctc cggggatgcc tgtaatgctt tctgcaacca
    34621 tagcagacaa ctcggggcgc atcctgtccg gccagacttt ggaagcgttc cttgtttcca
    34681 cggctcatgc cggattgttc agcgttggac tgaattgttc ttttggcccg gataaaatgc
    34741 ttccctatgt tcgcatactg tccagattcg ctccaggttt caccagcgca caccccaatg
    34801 ccggattgcc ggatatgttc ggcaaataca ccctttctcc tgaagctatg gcgcagcagg
    34861 taaagcctta tgttgaagaa gggctggtca atatcatagg aggatgttgc ggcactacac
    34921 cggaccatat tgaagcactg gccgggatgg tccggaagaa ccaggccccg ttgagaagag
    34981 tgccggtcag ggtagaagaa ggccagccgc aaaggattac cctgagcggt cttgagatgc
    35041 tcacagtaag ggaagagcct gcattcctga tagccggaga acgtacaaac gtgtctggaa
    35101 gccggaagtt cgcccggttg ataagagaaa agcaatacga ccaggcattg gaaatagcgc
    35161 ggggaatggt ccgggacggg gcacatatac tggatgtcaa tatggatgat cccatgttgg
    35221 atgcacccac agccatgagg gaattccttt tcatgctggg ctccgatccg gaagtggcca
    35281 gggtgccgct tatgctggat tcttcccgat gggaagtgct gcttgcgggc ctgcagtgtg
    35341 ttcagggcaa atccattgtg aattcgatca gtctgaaaga agggccagaa gcatttttgc
    35401 gaaaggcaaa actgataaaa caatatgggg cggttccgct tgtcatggct tttgatgagc
    35461 aggggcaggc atccacccgg gaacgccgca tagaaatatg tacccgggcc tttcgttttc
    35521 ttgtggacca ggccggtttt gagccccatg atattattct ggatcccaac gtgctggcgg
    35581 tgggcacagg gatggaggaa caccggcatt ttgccgtgga tttcatagat acagtccgtt
    35641 atctgaaaca aaacctgcaa ggagccctgg tcattgccgg ggtaagcaac ctttcttttg
    35701 cgttccgagg aaacgaaacc atccgggaag ccatgcatgc tgtttttctc tatcatgcca
    35761 tagcggcagg actggatatg gccattgtca aacccggagc acagcccata tattctgaaa
    35821 ttccggaacc tttgcgccgg gctgtggaag atgtgatcct ggacacagat gcaggggcca
    35881 catcccggct catagaaatg gcagcaacca tcaacggtcc gggacaacag gaagccggga
    35941 ccgtgacccg cgaagcctgg cgggacctgg atgctgagga acgcctgcac catgcactgg
    36001 cacacgggat agaagaatac cttgaagaag acctggaagc cctgtgcacc accccggcca
    36061 ttgatattgt ggaacatacc ctgatggaag gcatgaaacg ggtaggcaaa ctttttggag
    36121 agggaaaaat gttccttccc caggttgtca gaacagctcg gaccatgaaa agggcagtat
    36181 ccatattaca accccgcata gaggcggaaa agaacagcaa gacttcgggg aatgcagcaa
    36241 cggaaacggt gaaaggcatc attgccacag tcaggggcga tgtgcatgat attgggaaaa
    36301 acctggtgat cctggtaatg gaatgtaaca attacaaggt gagcgacctg ggggtcatgg
    36361 tgcccgcaca caagatagtg gaagcagcca tacgtgagca ggctgccttt gtaggattga
    36421 gcgccctgat cactccctcg ctggacgaaa tggttgatgt ggcaggtgcc atggaaaagg
    36481 caggactgac aacacccctg ctcattggag gagccacgac ttcatccctg ttcacctccc
    36541 tgaggatcgc gccgctttac agcggaccgg tcatccattg ccccgatgcc gccggtgtgg
    36601 tgccggtcct ggccgcttgt acctcaaaag atcccgtgga acgcgaacgt tattttacag
    36661 atttgcgcgc ttcccagaaa aggatgaggg atgcctacgg catggccgca gccaggaagg
    36721 aatacctgtc ccccgccgag gctcgtgcca acaaatgggt cccttctctc tccctggcag
    36781 ggcaggaatc ctgtccatgt tgcgggcagc accgggtgcc tgatgatttt gttcctcctt
    36841 tcatgggagt acagcatgtt gagattccct ttgcaaaact ccgcaagcat ttggactggg
    36901 atactttcct gcacatatgg gaggttcgtg aaagcaggga acagggaaag attgccgtgg
    36961 aagatgccgt ccgtctgctg gatgatctga ttggcggaca ttacggggac attgcaaagg
    37021 tccgggcagc cgccggattc tggcctgccg tatccactcc ggatgatcat atcttgtttt
    37081 ttgcagggga ttcctccctg catatcgtcc tggatttacc catggagcgt gacctgttgc
    37141 gccatgcgga cggatcgccg aatacaagcc ttgccgattt cattgctgcc gcggatcccg
    37201 cacacactcc ggatcatgcc ggcttgttcc tgctgactgc ctcctccccg cagctggaaa
    37261 aacttgcgga aagcatgcaa aagcgcggag atgtctacaa tgcgcttatt ttgagaacat
    37321 tgctggacct gctggcagaa gctgccagcg aagccctgca cagggagctt atggtttttt
    37381 cctgcggtgc acctcgcggg attcgtccgg cctttggtta tccttcctgt cccgaccaca
    37441 ccctcaagca aagcgttgtg accatgctgc aacctgcgga tgaactgggc attgccctta
    37501 catcgtcctg catgctgcaa cccgccgcat ccgtttgcgg catgtacatc agccacccgc
    37561 aggctcatta tatcactgta cacaaggatc cgggcctatg aagctgaccg atatattcaa
    37621 ggtttccgag aaaacttttt cctttgagtt ctatcctccc agggatgaaa tcgcagccgt
    37681 tgattttgga ttgaacatcg gacagcttat caagctgagt ccttcctttg tttctgttac
    37741 atacggagcc ggaggctcca cacagtcccg tacctttgac ctggtcgatt acttgaaaaa
    37801 cagggtagga ctcaataccg tggcgcatta tacatgtgtg ggcagcacaa ggtcaaggac
    37861 actggaagac ctgaagacgc tgcgcaagct tggactgcag aatttcatgc tgttgcgggg
    37921 ggatccggaa gtgggttcca agtatggttt catcccctgt tacgacggtt ttgccaatgc
    37981 gacggaactc atcacttttg cccgcgagtt cattgatgat tgctgtatag gaggaggagc
    38041 ctatcctgaa gtccatcccg aatcacccag tgcagaaata gatatgagga acctcaaaaa
    38101 gaaagtcgat gccgggtgcg atttcctgat aacccagttt ttcttcgaca ataaacggta
    38161 ttttgatttc gtttcccggg cacgcaaggc agggatcaag tgccggatca ttcccgggat
    38221 cattcccctg accaaatatt ctcagctgga gcgttttgtt aacctttcgc gtgcctcggt
    38281 gccacctgct ttcctggagc aactggaaag caaccgctac aatccggaag ccgtttacaa
    38341 agccggaatg gactttgcca tccgccaatg ccgggacctg ctcatgctgg gtgcccccgg
    38401 tcttcatttc tatacactca ataagtcaag ggcaaccgta gagatctatc agactctcat
    38461 ggtcaggtaa tagtgccaga gcaccactcc aatggccacg gatacattca gcgagtgctt
    38521 ggtgccatgc tggggaattt ccagacatcc gtcacaggca tccacaacct cctgtttgac
    38581 tccgcgcacc tcattcccga ataccagcgc cgttttctga tgttttccag gactgaaagc
    38641 cgccaggttt cccgcccctt cggcctgttc cacggccagt acggcatatc cctgctcctg
    38701 cagccggcgg acggcatcca gggtgttttc aacgtatgtc cactccatgg tgttttcggc
    38761 cccaagggcc gatttatgga tctctgcgga gggcggacac gcggttatcc cgcacaaaaa
    38821 gacatgttcc agggcgaatg cgtcggcagt gcggaaagcc gagccaatat tatgctggct
    38881 ccggacgttg tccaaaacca gcactacggg catcctgggg ctgttgcgga actcaccggg
    38941 ggataaacga cggagttccc tgtttggaat ttttctcggc atatggcaaa tgtacaggat
    39001 tttagtgtaa atttgtacta tgccaaccat gtatgtcata agcggctgca acggatcagg
    39061 taaaacaacc gcttcctata ccattttatc tgaattgctt cagtgcaggg cttttgtgaa
    39121 ttcagacgaa attgccaggg gtatttctcc ttttgacctt tcaaatgccg ccatagatgc
    39181 gggaagggtg atgctcaaaa gggtcaggga attgtcggaa tccaaggagg attttgccat
    39241 agagacaacc ctggctgtac gttcttttgc cagtctgata gaaaaaacaa agaaaaaggg
    39301 atacgacgtg atgctggttt atttctggct caaaagcccc gacctggcgg tgaaacgtgt
    39361 acaaagccgg gtgatggccg gggggcacac catccccgaa aacgttatac gccgcaggta
    39421 ctggtccggc atccagaacc tgttcaacat atatataccc ctgagtgaca ggtggttgct
    39481 tataaacaac tcggatactc cttcctccct gatagccgaa ggacacgggc acagggaaat
    39541 caaaatcttc aatccggaag attaccagac gcttaaaaaa ttatcgcaaa accccctccc
    39601 gggctcaggt aaaccttgat ctcatccttg gaggtaactt cccgctcttc tatcctgtaa
    39661 tctgttgcat gccggtgtgc gttcacaccg tccctgaaga ttttcgcccg gtaggtgcgt
    39721 cccggttcca gaaaggataa atccagggtg atggtgcgtg gcgtccagtc tgtgaggcct
    39781 cccacaaacc attgagagtc ctttctgcgt gcagatacta tgtattttcc cagttctccc
    39841 tgcaggatgc gtgtttcatc ccatgcaacg ggaatgcagg tgatgaaact ggtcgtttcc
    39901 tgttctttca tatatgtgga agggttgtca cataacatga ccaggggatt gtcaaagaca
    39961 atgtaggtgg ctacctgcct ggcccgtgta ccggcagcca tggggttgtt gtataccgga
    40021 tggaaatcct ggcgtgttgc attgcgcatc gccccctggg tgtagtcgac gggtccggcc
    40081 atcattctta tgtaagggaa agtgacttcg tgagggacca tgtcttcacg gctccacttg
    40141 gattgttcca gtccgaagat gccttcaaaa ttgaccacgt tcggataggt gcggttgaat
    40201 cccgtgggct tgtacatgcc atggaagtca atcagcatct tgtgcctggc ggctgtttct
    40261 gcaatccggt acaacatttc cactacaggc tggtcatccc tgtccatgaa gtcgaccttg
    40321 aatcccttga aacccattcg ggaatagaag ctgcaggctt cttccagctt ttcatccaga
    40381 acataggcta cacaccagag tatcaggcca acgttctttt tttcggcgta tgtaaccagt
    40441 tcctgcaggt caatctgcgg gaccacctgc atgatgttcc gacctgcggg aggcgaccat
    40501 ccctcgtcca gcaccacgta ttcaatgccg ttggcggcag caaagtcaat gtaatatttg
    40561 taggtttccg tgttgatccc taccgggaaa tccaccccgg tgacacccca gttattccac
    40621 caatcccagg ctaccttgcc tggtttgatc caatctgtgg cgcctgtcct gttgggagca
    40681 cccagcgcat agaccaggtc gttgacaggc aattcggtgt ctttttcagc tatagccatg
    40741 atgcgccatg gaaaagaacg tgttcccggg gtgcgtgcta tcacccggga ataatccgtg
    40801 gggattacct gtccgcgtac cggctcggtc ctggttgcgg aagggatggg tgcgaatacg
    40861 ccgcggtatt caaaaccacg gctggtcttt tctccccgca ggaacatgcc gggataagac
    40921 tccaggtcag attctgtaag aaccatcttt atgccgtcgt ccaggcagac cagaagcggc
    40981 aggaaggcca ggctatcagg attaaaaccg ctgacagggg taactgtgta tatgttttcg
    41041 aatgaattat taaaagggct ttccactccc cggctgtaag gaacgtaaca ggtgtaatct
    41101 ttttcgaaca caaatccggc cgattcgttc actatgtgca gggaatccct tccagtggta
    41161 tagaattggt aagcagctcc ctggtcatac aggcggaaac gaatcccgaa tccttcacga
    41221 aaactgatat ccaactggtt atacactgct attatttctg atttcctgta cagcggggac
    41281 gggatgattt cccgtacatt ggttgttttg gccctggaaa gcctggcatt gggtcccagg
    41341 attgttccgt cttccaggct gagtgaaagg gcgggggatg agacaaggac ctggtctccc
    41401 cgcagcaggc aaaaagagat gttgggctct gttaccgcct gaagttccag tttcccgtcc
    41461 ggagacgaaa tggagtggac tttaactttt gcttgcagga tggtgcccgt cacgcaggtg
    41521 aggaatgtga atactgccaa ggtcagcatc cctgtagccg gtttggtttt catatcagta
    41581 attgtttttg gtttcggaaa tggtaaatgt aaaggcatag ttgccataag gcagcatgta
    41641 caccgggcgg gggagtgccc cccaagtgtt gacgcacccc agccccatct ggcgaaggtc
    41701 aatgcagcat tgcgtgaaat tttcctcctt caggtcatgg gaatggaggt tgcgcttggc
    41761 cggaccggaa tccaggcttt ctatggaata atgaagcgcc gatgcggaaa acagcgagtc
    41821 tgaatctaca cggataccgg accctgtatg gtccagcagt tcccaccacc ggaggcctgt
    41881 ccgtgttccg ttttcctgag gacgtatata cggatagaac tgctcagaga cactctgcct
    41941 gtaaaggcct gtaaaagcag cggccgacct gtcgggataa ttttcaccgg gtcccctgcc
    42001 ataataaata aggcggttgt atttttttgg agtgttgaac ctgaccccga agcggaacat
    42061 cgggggaatt ttcccggctt cttcagcagt tgccagcgct tccagttcct gcgtgacctg
    42121 cagcccgccc tgtggcgccg tgcggtacgt catggtcagc ctagacttga gctgaggcag
    42181 gtcatataca gcggtaatgg catcatccgt tgtttcaaac cccttgaatt ccatgaccgg
    42241 atcacgccac gcgacgtatt ttaactgaag caatgcgcca aaatcgttat cggtcggggc
    42301 acgccagaaa ttgggggtta tccgtgaatt ttcatccagt atgctgatgc cactgaatga
    42361 aagatgggac acaaaaccat cgcgacggct gaattccacg cgcatgttcc cggagcgggc
    42421 gataagccag tttctgtcgt tatgctccat ggtgaaggac tgtacctcct gtccttcctc
    42481 cggccgcttt gccggttccg gcgagtgaac agggagctgg gctcttgcca gcacaatgcc
    42541 cgccgggaga agggcctcgg cctgaagggt ggaaaagtaa acgttcagga acacctcctg
    42601 gtcagagtac gatgaaagga aggtcttcct gtagcccaga tccaccacgc ttgtttctcc
    42661 cggaggtgtt ttcaggtcat ctgcataacc tttcttcacg gcaaccccgt tggccaccag
    42721 ctcccattcc agggaaaggt gtgagaggtc ccggaaaaag tattcgttca tcacaccaat
    42781 cctgccattg gccacatcca catcctgcac ccatacgttc tggtaatggt atcccacttc
    42841 ataagcgtgt ggattgggaa cacgggcagg gctgaacagc ccgttgttgt tgaaattctg
    42901 atccgaggca tcgtaaggat tgaaatcgcc cccgtaggca tacacatagt gtccttcctt
    42961 ccgatccagg aaaagagcct ggtctacaaa atcccatatg aatcctccct ggtattgggg
    43021 gtacttgcgt atgatctccc agtaataatc gaaacctccc atggaattac ccatggcgtg
    43081 agcatattca cattgtatca ggggcctttg gggattgttt tccaggtaac ggatacagct
    43141 ttcgtagccc atgtacatgg gggcgaaaat atctgatatc tcgggacggg tggcccgttc
    43201 gtactggatg ggcctttttt gaatatccgc ctttcgcaac aggtcataac ccatggtgaa
    43261 attttccccg tctccggcct cgttgcccat ggaccagatg atcacgctgg tgtggttctt
    43321 gtccctcagg tacatccttt ccatgcgctc tgcatgagcc ctttggtaat cggtgcgggc
    43381 ggccagagtc ctttccccgt aacccatgcc gtgcgattct atatttgcct catccaccac
    43441 gtagatcccg tactgatcac acaacctgta ccataggggt gaattggggt aatgactggt
    43501 ccttacggca ttgatgttga atcttttcat cagtgcaata tcctgaatca tgcgttcttc
    43561 agacaccaca taaccagttg cgggatccat ctcgtgccgg ttcacccctt tgatgagtat
    43621 gggttttccg ttgaccagca attgggcgtt tttaatctca acgctccgga agcccacctt
    43681 ccatggaatg aattctgtct ccttgccggt ttccgtgtcg tcaagcgaca ggagcagggt
    43741 gtagagatgc ggcagttctg ccgaccagtt ttttgtgttt tttacagtga atgagcctgt
    43801 ataggtattg ctttcacctg atgacaaagt tttttgtccc aggctgacac cctgcgggtc
    43861 aataagctcg gcgcgtacac gtgtcccggc agacggactg ttcaaggtca cggacattcc
    43921 cagggtgccg tttctgtaat tgtcatccag tacaggatgt aatatgaaat catgcatgtg
    43981 tgtcttttca cgtgcataga gaaatacttc gcgacccatc ccgctcagtc gccagaaatc
    44041 ctggtcctcc aggtatgtcc cgtcacacca gcggttcact ttcagggcta tgaggttatc
    44101 tcccgggcgg aggaaaggag tgatgtcaaa ttcggcttcc agtttgctat cttcagaata
    44161 cccggcaaac atcccgttga cccacaggct catgttggaa gtgaccgacc cgaaatgaat
    44221 aaaaacctgc cggccgttcc attcctcagg cagagagaag ttgtggcggt aataccctgt
    44281 atgattattt tccaggggaa caacaggggg agccgaccgg tactggttgt accatgcgta
    44341 attaaagttc aggtaaagag ggtggccgta tccgttcaat tcccacagac cggggacagg
    44401 aataatctcc caggacctgt catcgtaaca tgactgctca aacccggtgt aacccccgtt
    44461 ggcatcatga gtccatgcaa atttccacaa accgttcagg tccttgaagt atcgggagga
    44521 ttcccttttg ccgtcaaggg ccagggagcg tgactcatag gcatgatagg aagtacgcat
    44581 gggtactcgg ttgatctggt tcacttcagg atcctgccag acttccccgg ctttatacaa
    44641 aggataatca gtggtaatga aatccacgcc ggattggatg aaataatgaa aatcctcggg
    44701 ccggttaacc gtccagacgt tcacgtcaag acccagttcc cgcgcccgcg gaatccactg
    44761 cggatgagcc ttcagcaccc tcatgttata gtccagtccg gtgtatccct tttccttgag
    44821 ctggtcggga gacaagttgc cgcccaggta agccacccgg gcttccggat ccgaatggat
    44881 cagtgtttcg cagatgtgga aagaaaaact gatgtattcc actttattcc ggacacctgc
    44941 ctgtgatacc aattccagga tcctggcaac cgctgccttt tctgtatcct ggttcctgtg
    45001 agattttatt tctatgatta gtctggtgtg gtccgatgca gcgattaagg ccagaatttc
    45061 gtcaagcaac ggaagggttt ctccgtttga taaacgatag ccggatagtt ctgcataaga
    45121 acttgcatcc acacgtactc cgttcaagac aggatcgtgt accagaacca cttttccgtc
    45181 ggtagtcagg tatacgtcaa tttcggctcc gtaacaacgg gcatcgatgg cattttgcac
    45241 ggaactcctg gagttttgcg ccgagccttc cgccctccag aaaccccggt gggctatgat
    45301 gcccggttgc gaccaaaggg agagagaagg gatgagagac aggataatgg aaacaaagag
    45361 atagcgtaac atgttgtata attttgtaaa ttgcacgtcc atttgagatt ctgatatgcg
    45421 aatatacgat atttcacagg aaattttcag cggcaacata tttaaaggag accctgttcc
    45481 ggcagccacg cccctgtatc gcatccaaaa cggcgacccc tataatttat cttctgtatc
    45541 catgtgtctg cacagtgcca cacacattga tgctccttct cattacctgg aagacgggct
    45601 aaccatagac caggtcaggc tggaacactg tatcggcccc tgcacggtac tctctgtcaa
    45661 cggacctgtt gatgaagggc ttattacacc attattgaaa aggggtgcca agcgcctgtt
    45721 attcaagggt gaaggatggc tatcagttaa agcagccagg gcgttggctg aaagtgaggt
    45781 ggttatagta ggtaccgaaa gtcagaccgt tggttatgga aactatatag atgacgtaca
    45841 ccgggagttg ctgaagagca atatcgtgat cctggaagga ttggtgctgc aggatgttcc
    45901 cgaaggtaat tattacctga tatctcttcc tttaaaaatg gccggcatgg atggctctcc
    45961 ctgccgcgct gtcctgctgg attacatgcc accggtggac taatccgttc caaaagcata
    46021 tactattttc tgcaggtaca attctcccgg cctgagtata caatcaggat attgagaccg
    46081 gttggggctg tccgggtaat tctggcattc cagggctact gcgtgacggt ttctgtgtgg
    46141 tatcccgttt tttccttttc cgttaccttc cagccagttg ccggtgtata cctgtatccc
    46201 tggctgcgtt gtgctgatag tcaccgttct gccgctgacc gggtctttca gcagggctgc
    46261 ctgctgcaaa gtcccgtcag aagaaggcag acaccagcaa tggtcgtatc cgttgccaat
    46321 gcgtaactgt tcgttgtcca tggcaatgtc tttacctatg ggtttgggaa ccgtgaaatc
    46381 aaaaggcgtt cctttgaccg gggccggatc tcccagggga atactggtgg gatccacggg
    46441 caggtaattg ttggcattaa gttgcaggat atgattgtgg atggttccgt tgccttcgcc
    46501 cgccaggttg aaatacgtat gactggtaat gtttatgatg gttgacaggt ctgagatggc
    46561 tctcagttcc agtgtcagga catcattttc gctccagcgg aaagtggctg taacctgcat
    46621 gtttccagga taattttctt ctccgtgggg acttgcatag gtgaaagata cggcatcgtc
    46681 accatcctcg atgcgggaca cccagacctg tacacacatg gattgcgggc ccccgtgcag
    46741 gtggttggaa ccattgttga cggccagctg gtattgtttt ccttccaggg agaaaaggcc
    46801 ccgggcaata cggtttgcgt agcgtcctac cgtctttcca aggtacggag gatccaggaa
    46861 atactgcatg acgtcctgat aacccggtac cacatcttcc accagtccgt tgcggtcagg
    46921 cactcctatg cccatgaccg tagctccaat attacaaagt tgtacgtaag aacctttttt
    46981 attggtaatt ttgtaactga caattgcctc ccctttggga gagtagccat gaatatactg
    47041 cgtgacttcc ataatgcctg atttaaaaaa taaagataca aataatatga aaaaggccct
    47101 gaaaatttcc acctgctttt ttgcattact gcttgcttcc tgccagccgt accatgatgc
    47161 accatcaggt ccggatcaat accttgccgg tatacataag atcatagacc ttaaccttga
    47221 tgagctgttc aatacgtttg gagtggagga ttatcccgag atcacctcaa aactggaata
    47281 cgttatttcc agcaggaatt ttcgtgcagt tgtcatcaca taccacacga cagacccttt
    47341 cggggagcct gtgattgcag acggaacgct gtattacccg ctggattgtg atatcagggg
    47401 cgtggtggaa atgtcgggga tcgcccatat gagcaaagac ggcggaagct ctgagaatat
    47461 tccggttctg gaggctttgc cggtcctggt cggttacgcg gtaatcctgc cggatatgat
    47521 aggttatggc aaatacggca ataccaagga gatgcctcat cctttcatga tctgtgacaa
    47581 cctgggcaag gttgcgtggg atatgcgcat ggctgccact gaatatttca gaacactcgg
    47641 atacaacgtt cccaggcaca cgatcatttc agggtattcc tatggaggag gtgtgggatt
    47701 ggcggtagcc cgttattacc aggaacatca catgcatgag gtatacgttg aaagggtagt
    47761 gataggcgga ggtgcctgtg acctgaacgc cacattcaag gggtttgccg ataatcccat
    47821 ctgtacctac ccccttttac ccgggataat catgtccctt gaccattatt ataacctgag
    47881 cctggattac cacaagatat tccagggacc tctggccgat aattatgctt tatggtatga
    47941 cagaacgcgc attgccagcg ggctggtttc attgatagga gaagatatgc gcaattacat
    48001 gcaccccgat ttcttcaagc cggcagagga aaggaattcc gaattccaaa aactccaacc
    48061 tgttctggat atgaattccg ccgttgacgg gtgggttccc tatatgccgg tttacctcta
    48121 ccattcaacg gaagaccagt acgttccgga agaggcggca aactatgcgt acaggcaatt
    48181 caggaaagcc ggagcttata ttcaatacaa atcaggcccc ggaacacatg cagactgggg
    48241 ggtagagact tttgccgggc tgctgctcca tctggcaatc aagtaacagc atattcgtct
    48301 attatccgga ggatcacctt ggaagctttc tccatgcttt tcagtggaag gtattccatt
    48361 tttccgtgaa aattatgccc tcctgcaaaa atgttgggac agggaagtcc cctgtaggaa
    48421 agctgtgccc cgtctgtccc gccgcgaatt ggatgcacta tgggagtaat gcctgcagct
    48481 tttatggctt ttatggcttt ttccaccaca tgcatgtgtg gctctatctg cctgcgcata
    48541 ttgaaatact ggtccttcat ttccagggta atgcaattac cgtatttcag gttaaggaaa
    48601 ccggcgacat cctgcatgag ctgcttcttt tgttcaaatt tcccgtgatc gtgttcccgg
    48661 atgatgtaaa tcaaagaagc ttcttctacg gttccccgga atgatgtaat gtggaagaat
    48721 ccctcgtaat ggtctgtgaa ctcgggccgt tgctctaccg gtagcatgtt gttcagttct
    48781 gttccaatga taagggcgtt taccatcttg cctttggcat atcccgggtg gatattgcgt
    48841 ccctggatgt gaatgctggc catggctgca ttgaagtttt catattccat ttctcccagc
    48901 tggcctccgt caatggtgta ggcataggcc gccccgaatt ttttcacatc aaattttgct
    48961 accccgcggc caatttcttc gtcgggtgta aatgcgatct tgaccggtcc gtgttttata
    49021 ccgggatgcg tcataagata ttcggcggca gacatgatgg ctgccactcc agccttgtcg
    49081 tctgctccca gcagggtatt gccgtcggtg gttatcaggg tctgcccctg ataggaaagc
    49141 agttccggaa attcagccgg atccaggtac aattcctttt ccttgttcaa caggatgggt
    49201 ttcccgtcgt atttgggaat gatctgcggc cggatatcct tcccgctggc atcgggggag
    49261 gtatccacgt gtgcaaggaa gcctattgcg gggacttctt tttccaaatt tgaaggaatg
    49321 gaagccatga cgtacccgta ttcatccaga tgagcgtctg taatgcccat ctgatgcaat
    49381 tccttgagca gtacctccag cagggcacac tgctttgtcg tgctgggctg tgtttctgac
    49441 tgagggtcag aagccgtgtc aaaggagata tatctgagaa atttatccag tatcttttcc
    49501 atgatagatg tgtttatctt tcttttgcgg ccctcatgct gctgatggcc aagtatatga
    49561 ttatgcatgc ataagctgcc agtgcaagga gctcaccggc cgttgaatgc tgccactctg
    49621 cattgaccag attgatcccg gcaaagcgca tgatggcact caccacaccc atgagcgcac
    49681 ctcctgcaat aaagccagaa gcgagcaggg tgccacgatc cttacgttct tcgttaacca
    49741 ggcttttctt gctgcgtgtc gaaacaaacc aggctacagc tccacctacg agcaaaggca
    49801 ggttcagttc catgggaatg aacattccca gtgaaaaggc aagcgcggga accttgaaaa
    49861 gtgtcagcag gatggcaatc aggccgccaa tcccataaag gagccacgga gcacctcctc
    49921 cggacatgag cggttcaatg accgcagcca tggcgttggc ctgaggagct acaagcgcat
    49981 tttccccggt aaaaccg
//
LOCUS       DAOSOF010000002        31344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1466_length_31344_cov_6.056058, whole genome shotgun sequence.
ACCESSION   DAOSOF010000002 DAOSOF010000000
VERSION     DAOSOF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1466_length_31344_cov_6.056058"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1211
                     /gene="ruvB"
                     /locus_tag="PLM68_00230"
     CDS             198..1211
                     /gene="ruvB"
                     /locus_tag="PLM68_00230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT53830.1"
                     /translation="MADFDPHSNKETIDRDFEEQIRPREFTQFSGQDKITDNLKVFVK
                     AAILRDESLDHVLLHGPPGLGKTTLAHIIAHEMGVGMRITSGPVLDKPGDLAGLLTGL
                     EPRDVLFIDEIHRLSPVVEEYLYSAMEDFTIDIMLDKGPGARSVQISLQPFTLVGATT
                     RSGLLTSPLRARFGIQCHLEYYDAPVLLQIVQRSASILQVEIEHEAAREIASRSRGTP
                     RIANSLLRRVRDFAQVKGSGKIDTAITQYALNALNIDKRGLDQMDNKILSTIIHKFNG
                     GPVGIGTIATAVSEEVGTLEEVYEPFLIKEGFLQRTQRGREATKLAYEHLGILPAENK
                     TLF"
     gene            1223..2449
                     /locus_tag="PLM68_00235"
     CDS             1223..2449
                     /locus_tag="PLM68_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53831.1"
                     /translation="MNKSLFFTVLSILLISAATDAHACTSAIITGKVTANGKPMLWKH
                     RDTGQEQNRIAYFDSGKYGFLALTDAPDKGEEAWIGTNKAGFSIMNTASYNLKDDEVR
                     EMDREGKLMFRALEICAGLEDFERFLDTLSRPMRVEANFGVIDASGGAAFYEVNNHSW
                     VKLDVNDPAIAPHGYLVYTNFSYTGRINQGKGYMRYMTASDLFLEKSAIGGFTPQWIF
                     SHVSRSFYHAFLGIDLCDPAFSTQLAKGWFIDQDFIPRRSSTCSIVIEGVKPGEDPLN
                     TIMWTVLGYPPAGVCVPLWVNMESRQPALLVGQGENNRSPLCEKAVGLKHRVFSVERG
                     NGPCYMHFSLIYNSEGTGFMQQLTILEGSLFEKYNALIDSLEKEGMTGKKLAKKSVGE
                     MYKEVSALIEEVYDRL"
     gene            2436..5600
                     /gene="mfd"
                     /locus_tag="PLM68_00240"
     CDS             2436..5600
                     /gene="mfd"
                     /locus_tag="PLM68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT53832.1"
                     /translation="MTDFDVQEIQQFLEKTSPRHIPGILSLKGLTGSAVYFPVASLVK
                     KTPGKIHVLIREDSTEASYAAADLSVLLGYENVYLFPASYRGTGKTARPDESFQVQRT
                     MALRAVAQFYREPADILLVTYPKALQEGIPEKSLLETGHLAIRQHQALSHQDIKEYLF
                     SAGFEKTDFVSEPGQFAVRGSILDVFSYSENRPCRINFFGDQVENIRIFDQNTQLSIE
                     ERATIDIFPRLQGERSLLDVIENQALVWSFLESFRDIPQQIPVILVNPLTEEGSKVLY
                     DTTPQPVFNKNFNLLVNDISLKKENGYDVFILSENKAQIRRLEGIFQSLDCPKDFVRY
                     REISLHEGFTDHGNKICYYTDHQLFQRIHRVYLKRSVQKSEQITINDLSGFKIGDYVV
                     HIDHGVGVFGGLVKMVNNGREQEAVKLIYRDNDALYVSIHGLHRIARFKGKDSEPPRI
                     SKLGTGAWNKIRQQTKKKVKDIARDLIQLYSERISAKGFAFSADTYLQQQLEASFLFE
                     DTPDQLLATEQVKADMEKPYPMDRLLCGDVGFGKTEIAIRAAFKAVTDGKQVAILVPT
                     TILALQHYQTISERLADFPVKTRFLSRLKTPAQTSEILEQLKEGAIDIMIGTHRLLNK
                     DVIFKDLGLLIIDEEQKFGVAAKERLKHLKVNVDTLTMTATPIPRTLQFSLMGARDLS
                     IMQTPPSNRLPIHTEVQVFDEEIIGQAIDRELERGGQLFFVHNRILDIETIADILRQM
                     RPGLRIAIGHGQMPPRTMEKVLLDFICGDYDVLLSTSIIENGIDIPNCNTIIINMAHR
                     FGLSDLHQMRGRVGRSNKKAYCYLLVPGNTDLCEDAKRRLKALESFTELGSGLNIAMQ
                     DLDIRGAGNLLGGEQSGFIVQMGFEAYQRILEEALAELNPGAGDITVAVDCAIDTDLE
                     IMIPDDYISQVSEKIRLYKELDSIRDEDRLQRFFASLEDRFGPIPEQLRQLGYVVRLR
                     QLAVKTGFERIVLKNGIMLAYFISDQQSDYYKTQQFSDILNYISLNHKNLQVKEHNNK
                     LFLKIPHVRSAEAAYNFLKIFAKP"
     gene            5623..6930
                     /locus_tag="PLM68_00245"
     CDS             5623..6930
                     /locus_tag="PLM68_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53833.1"
                     /translation="MQKTGISILVFILPFLFIGTGKIQAQATMDALGTYTSYSMFGVG
                     ELLRPGTTLNRGMGGIGTGLRDNRYINYLNPASASSVDSLAFMFDFGLMQNNYINTDG
                     TLTTAYNAFNMHHIILSFPVWRRSAMQLGIVPYSNVGYRFIEDESRDEILANIGAYRY
                     YYYGQGSINQAFVSFSATFFRHLAVGAQGMFFFGNIEKHAQAISLSAPELYGTLKTGY
                     DIVIGSFGAQFGLQAFGNFSPSTTFTVGATFQPRASLRGDVTRFAKVEKGAFIDTVYS
                     QTSQGSQMSIPMEWSVGFSIRKRDKWVLGADYTQSDWRKSIFASTPGINFEPALSRTV
                     KAGLEFTPDRYSIRYYMRRWTYRLGAYYDKSYMMLNGNQVTAMGVTFGFSVPVYRWYN
                     SIAVSVDAGQRGSIENNLIRERYIMLILNFNLHDIWFIKHRYN"
     gene            6942..8282
                     /locus_tag="PLM68_00250"
     CDS             6942..8282
                     /locus_tag="PLM68_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53834.1"
                     /translation="MKKLGILFGFVMLAFVTQGYAQTGKYGATPEDSVECVRCLNFYK
                     EYYKNGNMKEALPSWRGALRVCPIGVTQSLYQDGQNILKFLINQTKDDVRRKELVDSL
                     ILMYDIRIQKFPAVKARLSAYTFKAYDMVTYFKEDDTKIFAALQDVVKFGQENTDQNI
                     LVLNMQYASSLFKEGKLTAEDVINVYEQISALFDKKTQTEAGEALAEARSSFETLFAL
                     SGVADCNNLLALFEPRFEQNATDINFIRRVVQLLSNAGCESSDLFFKAVQNLYKLEPS
                     YNSAYYLYRLCSARDINEDATGYLKSAIDCDSIDDITKGDYLLELGTFYYKKMNNYSN
                     AAHYALQSFEFNPATKGRAYLLMGHIWAMAKVRDGEMPDIDARANFWVAVDYFVKAKQ
                     ADPSLGEECDRLIATYRQYFPTVEEAFLHDLAEGSSYAVRANGISATTTVRTIK"
     gene            8282..8914
                     /gene="lptC"
                     /locus_tag="PLM68_00255"
     CDS             8282..8914
                     /gene="lptC"
                     /locus_tag="PLM68_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOT53835.1"
                     /translation="MFFPRRKTTVIMVATGLTLATMFVSCKNRMTTIGSLDNLDSIPT
                     QIVENMEGWQVEGGLMMGKLSAPYMEKYSRGTDPYEIFPRSLRVEGYTREGQLETVIT
                     ADRALHRSGDEQIWIATGNVVVRNLVKEETMETDTLYWDQQKKIIYTHCYVRLTSPDF
                     FAQGYGMESDERATNAKILRPFDSYGYIQKDTLSNEEKELTSRPETQPQP"
     gene            8925..11018
                     /locus_tag="PLM68_00260"
     CDS             8925..11018
                     /locus_tag="PLM68_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOT53836.1"
                     /translation="MALLEKIRVKMGIFITVLIAVALLSFIIDPGTLQSALSMFSSKN
                     DVGKMNRQGITYMEYAKKLEKFTNLQKAITGGSSLDEQSQEAVEQSAWQAFLKDLVYM
                     PAIQKAGISLGDEELFDMVQGRDISPVILNDPVFQGEDGQFDRTRLTMFVQNARTEPK
                     GIAALYWEFLQDNMETERIFTKYSSLLSKSNIVTPVELRRQMEENNITSNVSFSLTPV
                     SFARDTTIKVTSQEIRQYYEAHKHQYQQIATRDIEYVVFPILPSAEDFTLAQANIEKV
                     YEEFATSDNLRQFLARNSDKALDTYYYKKGELSSVSPKLDSFAFKATMRDVLPVYKED
                     NFFRAARIADIKQLPDSVYVHHILLQKTNKREATALADSLIAILNRTPGRFAELAQEF
                     SADNNPNAGPGEIGWFYQNRIVPGMESVFDAPLNKPYTLETQYGLHIVKVSERTRLHK
                     KVQLAVLVKEAVAGKDTYQAIYSMANSLSTESKDLEEFNRVAMQNNLMIIPAMNITQD
                     AKTISGYEHMRELVRWAFDAKYGAVSPVISVDNKYFFVAALNGIHEQGTSTLQEKQAE
                     IESILTLEKKQEKSYRQIREELAGHTSLESWAEATGRTISTQTGLAFGSTQQTDPKFV
                     GAVTAATEQTMYGPFKGEIGVYVFRVDERLDGAYYTENDAKQNAAYLSGVQLQMVPFV
                     LQEIAKVEDNRARFY"
     gene            complement(11015..12118)
                     /gene="ychF"
                     /locus_tag="PLM68_00265"
     CDS             complement(11015..12118)
                     /gene="ychF"
                     /locus_tag="PLM68_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT53837.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCVSSGKAQAANFPFCTIEPNLGST
                     QVPDPRLEQLASLVHPGRIVPATVDFVDIAGLVKGASKGEGLGNQFLSNIRETDAILH
                     VLRCFDNPNVAHVDGSVDPVRDMEIIDTELQLKDLETVENRLAKVQKQAVTGGDKQAA
                     RLAGLLSRYRDVLIQGKNARSVQLFNQEEEHMARELFLLTNKPVLYVCNVDEKSALTG
                     NHYTNRVTGILEKENEQYLIVAARIESEIAELESFEERQIFLESMGLEESGVSRLIKA
                     AFKLLDLETYFTAGPMEVKAWTYLKGTKAPQAAGLIHSDFEHGFIRAEVIKFKDYMHY
                     GSEAACREAGKMAVEGKNYVVQDGDIMHFLFSR"
     gene            12211..13146
                     /locus_tag="PLM68_00270"
     CDS             12211..13146
                     /locus_tag="PLM68_00270"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT53838.1"
                     /translation="MLRFIFCLVIFWNVENYFDPFDDPLTGDNDFTTAGSYTWTWNRF
                     LKKRNDIVKTLIACGEQEDGSWVAPCIVGLCEIENFFVLWQLVSDTPLSPLGYGIIHR
                     NSPDPRGIDVALLYRKERFSPVKTCFYGIPSEDGTLLKTREILYVSGVLDGRDTLHFL
                     VNHWPSRRGGDMAEANRQAAARLLQGIVDSIATASPGARIVVMGDFNDPPEAPVLRSL
                     SSDSLINVSAGDTYKYKGVWECMDQFLVSPTVNRELEYVKVARLPHLLERDQAHLGHK
                     PKRTYQGPAYRGGISDHLPITLKFITFVSYVIPKR"
     gene            13127..15736
                     /gene="mutS"
                     /locus_tag="PLM68_00275"
     CDS             13127..15736
                     /gene="mutS"
                     /locus_tag="PLM68_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT53839.1"
                     /translation="MSSRSVKTPLMDQYFAIKAEYPDAMMLFRVGDFYETFGEDAIKA
                     SKALGIVLTRRANGAASSVELAGFPYHALDTYLPKLVRAGYKVAVCDQLEDPKLTKKI
                     VKRGITEVVTPGLAYNASLLESSEHNFLASLYLHQEGTSESAGIAFTDISTGTIQIAQ
                     GDMAYAETLLSQYAPREILVQRGTEKKWKPLADHQAYFSTMDDWAFVHAAAEEKVKTH
                     FGVESLKGFGAENLPLAVCAVGAMLFYLEMNKYSSYGHLCTMQRIDKEQHMWLDKYSI
                     RNLELFPRPENNQKGASLIEVIDKTNTPMGARLLRQWLVMPLRDRTAIEERHNVVSSL
                     LEQWELAGKLRKLLSQVGDMERLISRAAAGRINPREAAQLRTGLNQVSRIRKLTAELP
                     QENPVYTLASTLDDCTGLVQTLQQMLVPMPPAQLGKGTVIAPGTDTALDELRHILSHN
                     KEYLQEMQQQAAAQTGITSLKIGFNNVFGYYIEVRNTHKDKVPPSWIRKQTLVSAERY
                     ITEDLKSYEERILGAEEKIAQLEQDLFNQVVDAIRATIPPIQENARILARLDCLLGFA
                     ILAREQKYERPRMQDDDVIDIRQGRHPVLETLMLPGEAYVPNDVYLDTGQQQIIILTG
                     PNMAGKSALLRQTGLIMLLAQIGSFVPVREATLGIIDKLFTRVGASDNIARRESTFMV
                     EMLESAAILNNVTDRSLVLFDEIGRGTSTYDGISIAWAIVEYLHEKPGASARTLFATH
                     YHELNQMAEQYPRVKNFHIAVKERGQKVLFLRKMISGGVEHSFGIHVATMAGMPRRVI
                     LTAQEVLKRMEAGKEQPPSPERPRQLSFFQLEDPLLQALKRDIESIDLNRLTPLEAFD
                     ALRELKRKIGQKE"
     gene            15733..17010
                     /locus_tag="PLM68_00280"
     CDS             15733..17010
                     /locus_tag="PLM68_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT53840.1"
                     /translation="MKKIFKSINIFFKLLGESFVMSYDSVKGDKFRAFLSLLGVTIGI
                     FSIVAVFTAIEALEKNVRNMFSALGTDVVYIDKFSWEAFTEEGGFKWWEYRQRPNNTY
                     EEFIFLQKNLNHADMVALSVYTTSNVGYERNVIRNASIQGVTYDWNKISYFELSEGRY
                     FTFAEAAENIPLVVIGQNVADELFPPGISPTNTNIKIGGFTARVIGVIKKKGESMFDP
                     ITYDDAVIVPLLFIRNFLDLRNTSPSIVVHRNPEADEEEFMAELRIAMRAIRRLKPIQ
                     KDNFALNKMSFLDDALSQLFKVISLAGWVIGGFSILIGAFGVANIMFVSVKERTHIIG
                     IEKAMGAKSSFVLAQFLFEAVLLAILGGMIGILVVWGLTIFVTRQYDFVMTMSMGNIM
                     RGVLISGLVGIVAGFIPSLQAARMDPVEAMNQH"
     gene            complement(16994..17797)
                     /gene="rsmA"
                     /locus_tag="PLM68_00285"
     CDS             complement(16994..17797)
                     /gene="rsmA"
                     /locus_tag="PLM68_00285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT53841.1"
                     /translation="MMEVRPKKSLGQHFLNDLKVAARIVDGLWPEKEMRSGAGIPVVE
                     VGPGTGVLTDFLLERPCLDYHAIELDRESVGFLQKKYPGLGSRLIEGDFLTAARSVFP
                     PRFLLIGNFPYNISSQIFIRVLEWRDSIPLVTGMLQKEVAERFCAPPGSKTYGILSVL
                     LQAWYDAEYLFTVPAGAFSPPPKVQSGVIRLVRNRRTALDCPEELFRKVVKTTFNQRR
                     KTIHNSLKPLLGEKKGFASPFSGLRPEQLGVEEFVALTQQVAAFLNAGS"
     gene            17848..19008
                     /locus_tag="PLM68_00290"
     CDS             17848..19008
                     /locus_tag="PLM68_00290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT53842.1"
                     /translation="MKRTVIIMAALLVLVSCSGYRTHKADIRLDELRQELNAVGLSVA
                     VISDNQVVYTGATGFRNMETREPLSSSDLMRIASISKSFTATAIMQLYEAGCFDLDDD
                     IGTALGFPVRNPHYPDIPITYRMLLSHTSSLSDSAGYFSLDVLRPDITKDVSPSFNDY
                     APGTAYQYCNLGFNTLGILVEIHSGERFDQYLWYNVLEPLGVYASYNVNDLDTTRFAT
                     IYSFEDGEFVPAPQAYNPRKEDIENYVMGSSAVVFSPTGGLKISAPDLARQALVQMND
                     GTLDGREVLTRESVALMQTPQSPGGRDTMAEGLGYGFALQKTDKLIEGEIMTGHTGSA
                     YGLYSAMFFEKDKKFGFVVLCNGCDTSIPNRDNGFMAVQTDVINALYDIFIR"
     gene            complement(19022..20785)
                     /gene="aspS"
                     /locus_tag="PLM68_00295"
     CDS             complement(19022..20785)
                     /gene="aspS"
                     /locus_tag="PLM68_00295"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT53843.1"
                     /translation="MYRTHTCGELDLSCAGQRVTLAGWIQRIRKLGGMTFIDLRDRYG
                     ITQLVVKEDSPVKVKETAESLGREFVVSAEGIVVERSSKNLKIPTGEIEIDLGGISVL
                     SPSQVPPFTIEEISDGGEELRARYRYLDLRRPPLQKAMILRHKIAQETRGYLDNCGFL
                     EIETPFLIKSTPEGARDFVVPSRMNQGQFYALPQSPQQLKQLLMIAGYDKYYQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFVERDDVLSVFEGLILHLFKKVHNLDFTAPLPRMSYK
                     TAMEKYGSDKPDIRFDMLINDLTTFTRDTEFSVFRNAAYVGAICVKGAAGYTRKQLDE
                     LTEWVKRPQVGGSGLVYVRCQAEGDGSVAYKSSVDKYFDQQTLARWTQQTGATGGDLL
                     LVMSGPETKTLEALGSLRLEMGSRLGLRNKDVFAPLWVYDFPLLEWDEETQRFYAKHH
                     PFTAPKPEDLDKFYSDDPRDLEQVNANAYDFVLNGTEIGGGSIRIHDARMQERMFRIL
                     GMTEEEVNARFSCIIDAFRYGAPPHGGIAFGFDRLCSLFGGSESIRDFIAFPKNNSGR
                     DVMLDAPAGIDALQKKELGLK"
     gene            20842..21636
                     /locus_tag="PLM68_00300"
     CDS             20842..21636
                     /locus_tag="PLM68_00300"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT53844.1"
                     /translation="MKNYLDLLQYILDNGTDKEDRTGTGTRSVFGYQMRCPLAEGFPL
                     LTTKKVHLKSIIYELLWFLRGDTNIAYLNRNGVTIWDEWADARGDLGPVYGKQWRSWE
                     TADGQVIDQIAALIEGLRSNPNGRRHIVSAWNVGQISRMALPPCHVLFQFYVADGKLS
                     CQLYQRSADVFLGVPFNIASYSLLTLMVAQVCGFRPGDYIHTFGDAHIYRNHMEQVHL
                     QLSRTPRPLPHMILNPDRHDIFGFEYSDFTLQDYDPHPLIKAPVAV"
     gene            21633..22148
                     /locus_tag="PLM68_00305"
     CDS             21633..22148
                     /locus_tag="PLM68_00305"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT53845.1"
                     /translation="MKSEHPSKPGILSLIAVLGPSGELGVRNGLLWHISEDLKHFKSL
                     TMGHTVIMGRRTFESLGKQLPGRTNIVLSRSGKKLEEILEDVKDQPEVFIIGGAQVYR
                     QAIHLADRLYITHVQAPLPSGGADVFFPPIDPRQWQEVSRQDFVRGAAFPYPFSFVTY
                     ERRKTQQQPPV"
     gene            complement(22069..24570)
                     /locus_tag="PLM68_00310"
     CDS             complement(22069..24570)
                     /locus_tag="PLM68_00310"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOT53846.1"
                     /translation="MQKIPVKKWIVYPVAAVFFLVLSYGFVPQIFQGKVLNQGDISAW
                     QAMAKEAKDYNQTHEDEALWTNSMFSGMPTITINKVTRGNYTRLISRVLEAGKAPASY
                     LFLSLVGGFLLFLALGVNGWLAMGGAIAITFCAYNMQIIQAGHVTKMIAIAYMPWVLA
                     SLVFAYRKNALWGALFFALTVSFQVAANHPQITYYLGFIMVFCFGGLLAEAIIRRRLP
                     RFVNTTVLLIAAGLLGLATSANHLLPMYEYTQYSMRGGSELENPEGEAPRKGLDVEYA
                     TQWSYSQMESFNMMIPDFRGGASVGALDQDSHSYEKLSGMGYNARSIISNMPLYWGEQ
                     PFTAGPMYMGAIMIFFFVLGLILVKGPLKWALAGVSLLALLLSWGYHLEWFSVLFFRY
                     VPLYDKFRTVSMILVIWQMVVPALGIYAVWNLLDNQYPQEKTTRALYWSLGITAGLCL
                     VFALAPSLAGSFTGPSDASLPGELLPALVADRKGLLRQDAFRSAFLIAGAALVIWLAV
                     HKKMRTVWAVAALGILLLVDYLPAARRYLNDSHFVTRSNYSNQFAERPVDKLIKGDPD
                     MYYRVLDLTGSPFSESRPSYHHKSIGGYNAAKLQRYQDVIDRHLLPEMQYFMEKIRQC
                     QTLQDASEIFAYQGEQTSTPVMNLLNTRYVILSGDAAPVVNPHALGNAWVVSSILPVE
                     TNLEELEMLSRADLRTTAVMRDAPEEWVQAASPSGSTVTLASYSPNTLEYNASMEKDG
                     IVVFGEVFYPKGWKAWIDGEEVPILRSNYITRTLLVPAGEHGIRFTYLPASYTTGILV
                     SRISSVGLLLTLAAAVVFSCVRMLRRKKDTGKQLP"
     gene            24635..25972
                     /locus_tag="PLM68_00315"
     CDS             24635..25972
                     /locus_tag="PLM68_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOT53847.1"
                     /translation="MKRDEFSGFFGVLVATVGSAVGLGNLWRFPYLVGQNGGAAFILI
                     YLAFVLVICLPIMISEFIIGRRTQANTVTAFQVLAPHTPWSVIGIVGVVAAFCIMAFY
                     TVVGGWTLDYIFRSVIHLFSKPDASAIESQFASLVTSPVRPIVWTIVFILLSALIVMS
                     GIKKGIEKYSKVMMPFLFLMVVILMIRSITLPGAEKGISFLVKPDFSKITTQAVLAAL
                     GQAFFSLSLGMGCMITYGSYIPRRENLNKLAFTTAMSDTLFAILAGLAIMPAVFSFGI
                     SPQEGPGLVFIVLPRIFAQIPLGNIFAVMFYLILFIAAISSAISLLEVVTAYITERLK
                     MKRKAAVAIISALVAVCASFSSLSQGVMQDVKILGKTIFDFFDYLSANILLPLGGLSI
                     VLFVGWYLKKTDIYDEFTNQGTHKGTLFRVYYFLIRYVAPIAIAAIFVDLIVR"
     gene            complement(25959..29636)
                     /locus_tag="PLM68_00320"
     CDS             complement(25959..29636)
                     /locus_tag="PLM68_00320"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOT53848.1"
                     /translation="MKKITSLLVIAATLLFISGCKPEVPYIEASPVSVIFPQEGGTQA
                     ISLSTNSMSWTASVSGKGFSISPASGAGDATLQLTAAASTSSSDQTGMLTIKSGTMQA
                     SVTITQSARNTLIVNGDNTVEASGGTYSLTLQYNTEYTVEIEEAAKSWIQYTGTKALS
                     TATLQFLITPNTGSPRSGKVSVVDKAGIAQTQTFTFNQKENALRTLLVQLYDNMDGNN
                     WVAEKKTNWKTEEPIDQWAGVTMEEGKIIQLNLSGFGLNGQLPAGIGTLTDLTKLTLS
                     SNPDLTGPLPSSLGDLANLDALLAVTTGLEGPLPKEMGNLKKLTNLQLNNNNITGTIP
                     SEWGDMAALKNFGMFNTKISSPLPNTIFENWKHIGTILLHSNPNLTGSLPLKLGQMET
                     DNTLFSVQMYNCNFEGGIPEEWGSMPTVSKQLHLYGNKLTEPVPLSIQNHPSWTTDKW
                     DKWKDAGTHFIRTQQNGIFLELEQVPDAQRDCLMALYNALDGPNWTKGTNWNTDAELG
                     TWEGVTVTDEAITGLSLSGFGLKGQLPPQIGELTALKTLNLGSNPNLTGPLPAELGNL
                     VNLTALMAVTTGLEGPLPAEMGNLNKLTNLQLNNNQISGTIPKEWGGMTALNNFGMFN
                     TKISGPIPSEIFTGWKNLATFLMNDNPNLTGSLPEGLGNMTTTQTRFNIHLHECNFTG
                     GIPASWGNLPAVSSQLRVYGNKLTKPVPAVIINHPSWTSDKWDSSKTNTEIHYIRTQQ
                     NGVFLELEGVLPTVGKVTVTELLYNKISVSAEVLKQGSHAVTERGFVLNTTTRQAGDG
                     TGIFSIIFTGNIHENTAYTIKAYATSQAGTAYGEEITVTTPRYNEVNMVLVDNTGASV
                     DYADVYLKLLSSEKRSASPAYVHTAGKALPVIKGRTQEEVLQSAGEWLTSLMKPYASQ
                     IRNNLEALKNGTLQVPAMRSTSIRPAAADDYDYKVTSAAGGSVQIKNVVPGTYGVRVN
                     GYGLVKDFYTMLTIPEGVSNIEVKDIPLLSVTSSTLSLIRPNAGIRPFSSPVADDNVM
                     VLVQMAPDFVKTWKGKILENIMLCPMDTTSSIVIMAKDENQLTEIYEIFISLVDEEGN
                     PQQPTDPTQYVNFIETLSRRIIMVQDASVAQTNLLSQSTMNLKKYFIDAIKATGKWDL
                     VKTFVTENYIVQIQDGDGVILNMIIAQKGTQPVLMTDGEGPAHEGGNLLLLTSEEEEN
                     TVTSLKDLGYNGNWHLGITVR"
     gene            complement(29671..30534)
                     /locus_tag="PLM68_00325"
     CDS             complement(29671..30534)
                     /locus_tag="PLM68_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT53849.1"
                     /translation="MGKIDYAAMKPVQPARKKVRDENESVKIDGMNGVKHVIAIASGK
                     GGVGKSTVAVNLAVALALEGYKVGLADADIYGPSVPILTGTQEMQPQVDAIDDNVQVF
                     EPVEKFGVKWMSIGYFARPGQALIWRGPMASNALKQLLQQTKWGVLDFLLIDLPPGTG
                     DIHLSLMQDIPLTGGIVVTTPQQVSVTDVEKCLNMFMHPDLNKRVLGLVENMAWFTPE
                     ELPDKKYFIFGKGGAEELAKKYNMPLLAQIPIVQSVCERGDAGIPVANDKNAAGQAFK
                     DLAGKLTSLLA"
     gene            complement(30539..>31344)
                     /locus_tag="PLM68_00330"
     CDS             complement(30539..>31344)
                     /locus_tag="PLM68_00330"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOT53850.1"
                     /translation="KHFALKVDKAYYPSVYGIIENEFSGNIDAYVEHLFEKSHFVSAE
                     RVNSLIECLSAENTREAALQEIASDPAVLLGQSVESRNAECQAVIAPCFLQYERGHRN
                     FIAGMMEMNKGKAMYPDANSSMRLTYGNVLNYKPKDGVIYDYYTTLTGVMEKEDPDNW
                     EFVVPEKLKQLYRDKDYGQYALPGQEIVTGFIANTDITGGNSGSPVLNADGELIGLAF
                     DGNWESMSGDVIFEPALQRCISVDIRYVLFIMDKYGEAGYLLKEMDIRK"
BASE COUNT         7942 a         7936 c         7844 g         7622 t
ORIGIN      
        1 aggtgattat tgtgcatatt tttaattttg tggacaaatc acgatttttg catttttgcc
       61 aacagggata ttaacatccg tttttgggcc attcatgatt aaacgtttga ttttcaaacc
      121 tattattttg ttttcaacat atcaacagac tattaaaagt aacaaaagac tataaatcca
      181 attatattta ttgcttcatg gcagattttg acccccacag taacaaagaa actattgaca
      241 gagattttga agagcagata agaccaaggg aattcaccca gttctccggt caggataaga
      301 tcacagacaa cctgaaggtc tttgtgaaag ctgccattct cagggatgag tccctggacc
      361 atgtcctttt gcatggccct ccgggattgg gtaaaacaac gctggcacac atcattgcgc
      421 acgaaatggg ggtagggatg aggatcacct cgggccccgt gctggataaa ccgggagatc
      481 tggcaggatt gctcaccgga ctggaaccaa gggatgttct tttcatagac gagatccatc
      541 gtctttcccc tgttgtggaa gaatacctgt attctgccat ggaggatttt acgattgata
      601 tcatgctgga caaaggaccg ggtgcccgtt cagtacaaat atccctccag ccattcaccc
      661 tggtaggggc cacaacccgg agcggcctgc ttacttctcc ccttagggca cgttttggta
      721 tacaatgcca ccttgagtat tacgatgctc ccgtgctgct gcagatcgta cagcgttcgg
      781 cgtccattct ccaggtggaa atagagcatg aagctgcccg cgaaatcgct tcccgcagcc
      841 ggggaactcc cagaatagcc aattcgctgt tgagaagggt aagggatttt gcccaggtaa
      901 aaggttccgg aaagatagac acggctatca cgcaatatgc gctgaatgca ttgaatattg
      961 ataaaagagg gctggaccag atggacaaca agatattgtc caccatcatc cataaattca
     1021 acggaggccc tgtcggaata ggcactatag ccacagccgt aagcgaggaa gtgggaacac
     1081 tggaagaagt atacgaacca tttcttatca aggaaggctt tttacagcga acccaaaggg
     1141 gcagggaggc tacgaaacta gcttatgagc atttaggcat attgcctgca gaaaacaaga
     1201 cactgttcta aaaagtaata ttatgaataa aagcctgttt ttcaccgttt taagcatttt
     1261 gttgatttca gcggccactg atgcgcacgc atgcacttcg gccatcataa cggggaaagt
     1321 caccgcaaac ggaaagccta tgctatggaa acacagggat acagggcagg agcaaaaccg
     1381 catagcctat tttgattccg gaaagtatgg cttcctggcc ctgacagacg cgcccgataa
     1441 aggtgaagaa gcctggatag gaaccaataa ggcaggtttt tccatcatga acacggcatc
     1501 ctacaacctt aaggatgacg aggtcaggga aatggaccgt gaaggaaagc tcatgttcag
     1561 ggctttggaa atatgtgcag gattggaaga ttttgagcgg tttctggaca cgttgtccag
     1621 acctatgagg gttgaggcca attttggcgt tattgacgct tccggagggg ctgcctttta
     1681 cgaggtcaac aaccattcct gggtgaaact ggacgttaat gaccctgcca tagccccaca
     1741 cggatacctg gtctatacca atttttccta taccggcagg atcaaccagg gaaaggggta
     1801 catgcggtat atgactgcca gtgatctttt cctggaaaaa tcagccatag gcggttttac
     1861 cccgcaatgg attttcagtc acgtttcccg ctccttttac catgcatttc ttggaatcga
     1921 cctttgtgat ccggcctttt ccacgcaact tgcgaaagga tggtttattg accaggattt
     1981 cataccccgc cgtagcagca cctgttctat cgtgatagag ggtgtaaaac caggagagga
     2041 tcccctcaac accatcatgt ggactgtttt gggatatccg ccggccggag tttgtgttcc
     2101 cctgtgggtg aacatggaat cacggcagcc cgccctgctt gtgggacaag gcgaaaacaa
     2161 caggtcccct ttgtgtgaaa aagcagtcgg cctcaagcac cgggtctttt ctgtggaaag
     2221 aggcaacggg ccctgctata tgcatttttc tttaatatat aacagtgagg gtacagggtt
     2281 catgcaacaa ctgactatcc tggaaggcag tctttttgaa aaatataatg cgctcattga
     2341 ttccctggaa aaagaaggca tgacagggaa aaaactggca aagaaaagcg taggggagat
     2401 gtacaaggaa gtctcggcgc ttattgaaga ggtatatgac agactttgat gtacaggaaa
     2461 tacagcaatt tctggaaaaa acatcaccgc ggcatattcc cgggatcctg tcgctaaagg
     2521 gacttaccgg ttctgccgtc tattttcccg ttgcatcgct tgttaaaaag acacccggga
     2581 aaatccatgt actgatacgt gaagattcca ccgaggcttc ttatgctgcc gcagatctgt
     2641 ccgtgttgct gggctatgaa aacgtatatc tttttcctgc ttcgtacagg ggaacgggaa
     2701 aaaccgcccg tcccgacgaa tcgttccagg tgcagcggac catggctctg cgggcagttg
     2761 cacagtttta cagggaacct gcagacatcc tgctggtgac ttatccaaaa gccctgcagg
     2821 aaggaatacc tgaaaaatcc ttgctggaaa caggacatct tgccataagg cagcatcagg
     2881 cattgtcaca ccaggatatc aaagagtatc tgttttccgc tggcttcgaa aaaacagatt
     2941 ttgtaagtga gccgggacaa tttgcggtca ggggaagcat cctggatgtt ttttcctatt
     3001 cggaaaaccg tccatgcagg atcaatttct ttggagacca ggtggaaaac atcaggatat
     3061 tcgaccaaaa cacacaatta tccatagaag agcgtgccac catagatatt tttccgcgct
     3121 tgcagggtga acggtctttg ctggatgtca ttgaaaacca agccctggta tggtcttttt
     3181 tagaatcatt cagggatatt ccacagcaga ttccggttat cctggtgaat cccctgacgg
     3241 aagaagggtc gaaagtctta tacgacacaa cgccacagcc tgttttcaac aagaatttca
     3301 acctgctggt aaatgacatc agcctgaaga aggaaaatgg ctatgacgtc ttcatactca
     3361 gcgaaaacaa agcacaaatt agaaggctgg aagggatctt tcaaagcctg gactgtccaa
     3421 aggattttgt ccgctaccgg gagatctctt tacacgaggg tttcaccgat cacggcaaca
     3481 agatatgcta ttatacagac caccagcttt tccagcgtat tcacagggta tacctcaaaa
     3541 ggtcggtcca gaaaagcgag cagatcacca tcaatgacct gtccgggttc aaaatcggag
     3601 attatgtggt ccacattgac cacggggtgg gggtattcgg agggctggtg aaaatggtca
     3661 acaacggcag ggaacaggaa gccgtaaagc tcatatacag ggacaacgat gccctgtatg
     3721 tgagtatcca cgggctacac agaattgccc ggtttaaggg caaagacagt gaaccaccca
     3781 ggatttcgaa gctaggcaca ggggcctgga acaagatcag gcagcaaaca aagaaaaagg
     3841 tcaaggatat tgcccgtgac ctgatacaac tttattcaga aaggatctcg gcaaaaggat
     3901 ttgctttttc tgccgatacc tacctgcagc agcagctgga agcctctttc ctttttgaag
     3961 acacccccga tcagctcctg gccacggaac aggtaaaggc cgatatggag aaaccgtatc
     4021 ccatggacag gctcctgtgc ggggatgtgg gtttcggaaa aaccgagata gccatcaggg
     4081 cggccttcaa ggcagttaca gacgggaaac aggtggccat ccttgttccc acaaccatcc
     4141 tggccctaca gcattaccag acgatttccg aaaggctggc cgattttccc gtgaagacca
     4201 ggttcttatc ccgcctcaag acaccagccc aaaccagtga aatccttgag cagctgaagg
     4261 aaggagccat agacatcatg atcgggacgc accggcttct caacaaggac gtgatcttca
     4321 aagacctcgg cttgctgatc atagatgaag agcaaaaatt tggcgttgcc gccaaggaaa
     4381 ggctcaagca cttgaaagtc aatgtggaca ccctgaccat gaccgctacc cccatcccca
     4441 ggacattgca gttttcattg atgggagcca gggacctgtc catcatgcag acaccgccat
     4501 ccaacaggct tcctatccat acggaagtac aggtttttga cgaggaaatc ataggtcagg
     4561 caatagacag ggaacttgag aggggaggac agctcttttt tgtgcacaac cggatattgg
     4621 acattgaaac cattgccgac atacttcggc agatgcgtcc cgggctcagg atagccatag
     4681 gtcatgggca aatgcccccc aggaccatgg aaaaggtatt gctggatttc atttgcgggg
     4741 attacgatgt gttactctcc acatcgatca tagaaaacgg gatagacata cccaattgca
     4801 acaccataat catcaatatg gcccatcgtt tcggactgag tgacctgcac cagatgaggg
     4861 ggcgggtggg acgctccaac aaaaaggcct attgctacct gctggtgccc gggaataccg
     4921 atctgtgtga agatgcaaaa agaaggctca aagcgctgga atcctttaca gagctgggaa
     4981 gcggcctgaa tatagccatg caggacctgg atatccgtgg cgcagggaac ctgttggggg
     5041 gagaacagag cggattcatt gtccagatgg gctttgaggc ttaccagcgc atcctggaag
     5101 aggcactggc cgagttgaat cccggtgccg gggatatcac tgttgcggtc gattgtgcca
     5161 tagacacaga cctggagatt atgattcccg atgattacat ttcgcaggtt tctgaaaaga
     5221 taaggcttta caaggaattg gacagcataa gggatgaaga ccggctgcaa cgttttttcg
     5281 cctccctgga agacaggttc gggccgatac ctgaacaatt aaggcaactg ggatatgttg
     5341 tcaggttaag gcaattggcg gtaaagacag ggtttgagcg cattgtgcta aagaacggca
     5401 taatgcttgc atatttcatt tctgatcagc agtcggatta ctataaaaca caacaatttt
     5461 cggatatatt gaattatata tcattgaatc ataaaaattt gcaggtgaag gaacataata
     5521 ataaactgtt tctcaaaata cctcatgtaa ggagtgccga ggctgcttac aattttttaa
     5581 aaatatttgc aaaaccctga aaaagtgcga catttgcaag ctatgcaaaa aacaggaatc
     5641 agtattctgg tctttatttt accgttcttg ttcattggaa cgggcaaaat acaggcacag
     5701 gcaacgatgg atgcattagg cacatataca tcgtattcaa tgtttggggt gggtgaactg
     5761 ctcaggcccg gaacgaccct gaacaggggc atgggcggca taggaacggg actaagggat
     5821 aaccgctata tcaattacct gaatccggct tctgcatcgt cggtggattc gctcgcattc
     5881 atgttcgatt ttggattgat gcagaataat tacattaaca cggacggaac acttaccacg
     5941 gcatacaatg ccttcaatat gcaccacatt atcctgtcat ttcccgtatg gagacgttct
     6001 gccatgcaat tggggatagt tccgtacagc aatgtgggat accgtttcat tgaagatgag
     6061 tcccgggatg agatactggc caatattgga gcctatcgtt attattatta cggtcaggga
     6121 agcatcaacc aggcctttgt ttcgttttcg gccacatttt tccgccacct ggcggtaggt
     6181 gcgcagggta tgtttttttt cgggaacata gaaaagcatg cgcaggccat atccctttct
     6241 gccccggaat tgtacggcac actcaagacg ggttatgaca ttgtgatagg atcttttgga
     6301 gcacaattcg ggctgcaggc attcgggaat ttttctccaa gcacaacatt caccgtcggg
     6361 gcaacattcc agccacgggc atctctgcgc ggggatgtca cgcgctttgc caaggtcgag
     6421 aaaggggctt tcatagatac agtttattca cagaccagcc agggatctca aatgtccatc
     6481 cccatggaat ggtctgttgg tttttccatc cgcaaacgcg acaaatgggt tttaggggct
     6541 gattatacac agtctgactg gagaaaatca atatttgcat ccacacccgg gatcaatttt
     6601 gagccggcat taagcaggac ggtgaaagcc ggacttgaat tcaccccgga ccgttacagt
     6661 atccgttatt atatgcgcag gtggacctac aggctgggag cttactacga caagtcctac
     6721 atgatgctga acgggaacca ggtgacagcc atgggtgtga cattcggatt ttcagtgcct
     6781 gtttaccgct ggtacaattc aatagctgtc tctgtagacg ccggtcaaag gggaagtatt
     6841 gaaaataatc ttatcaggga acgttatatt atgttaattt tgaacttcaa tttgcatgat
     6901 atttggttta taaaacatag atacaattaa aacatacaac aatgaaaaaa ctcggaatcc
     6961 tattcggctt tgtaatgctt gcctttgtaa ctcagggtta tgcacagacc gggaagtatg
     7021 gggcgacgcc tgaagacagt gtggaatgtg taagatgttt gaatttttac aaagaatatt
     7081 acaagaacgg gaatatgaaa gaagcccttc cttcctggag gggagcactc agggtatgtc
     7141 ccataggggt aacgcaatcc ttataccagg atggacagaa tattctaaaa ttccttataa
     7201 accagacaaa agatgacgtt cgcagaaaag aactggttga ctccctgatt ctcatgtacg
     7261 atatcaggat tcaaaagttc ccggctgtaa aagcacggct gagcgcatat actttcaagg
     7321 catacgacat ggtcacgtat ttcaaagaag atgacacgaa aatcttcgca gcgttgcagg
     7381 atgtggtgaa attcggacaa gagaacacgg accagaacat tctggtattg aacatgcaat
     7441 atgcatcgag tcttttcaag gaaggcaagc tgacagcgga agacgtcatt aatgtatatg
     7501 agcagataag cgcccttttt gacaagaaaa cacagacaga agcaggcgag gcccttgcag
     7561 aagcccgcag cagtttcgaa acactgtttg ccctcagcgg agtggctgat tgcaacaatc
     7621 ttcttgccct gtttgaaccg cgctttgagc agaatgctac cgacataaac tttataagac
     7681 gggtggtcca gttgctttcg aatgcaggat gtgagagctc cgacctgttt ttcaaggcgg
     7741 tacaaaatct ctataaatta gaaccgtcct acaactcggc ttattacctt taccgcctgt
     7801 gttcggccag ggatataaat gaagatgcca caggctacct caaaagcgcc atcgattgcg
     7861 attcaattga cgatataacc aagggagatt atttgctgga actgggcacc ttctattaca
     7921 aaaaaatgaa taattattcc aatgccgcac attatgcact ccagtctttt gagttcaacc
     7981 cggcaacaaa gggcagggct tatctcctga tgggtcacat atgggccatg gcaaaagtgc
     8041 gtgacgggga aatgcccgac attgacgccc gtgccaactt ctgggttgcc gtggattatt
     8101 ttgtaaaggc aaaacaggca gatccctccc ttggggaaga atgcgaccgc ctgattgcca
     8161 cgtacaggca gtacttcccg acagtggaag aagcattttt gcacgatttg gcagaaggtt
     8221 ccagctatgc ggtgagagct aacggcatat cggcaacgac tactgtcaga acaataaaat
     8281 aatgtttttt ccccgcagga aaacaaccgt gatcatggta gcaacgggtc ttacccttgc
     8341 taccatgttt gtctcctgca aaaacaggat gacaacgatt ggatctcttg acaacctgga
     8401 cagcattcct acccagattg tcgagaacat ggaaggatgg caggtcgagg gagggcttat
     8461 gatgggaaag ctgagcgctc cttatatgga aaaatattcg cggggaaccg atccctatga
     8521 aatcttcccc cgttcattac gggttgaagg gtatacccgg gaaggacaac tggaaacggt
     8581 gattacagct gacagggcat tgcaccgttc aggagatgag cagatatgga tagctacagg
     8641 caacgtggtg gtcaggaacc tggtcaagga agaaaccatg gaaaccgaca ccctgtactg
     8701 ggaccagcaa aaaaaaatta tctatacaca ttgttatgtc aggctgacct caccggattt
     8761 ttttgcacag ggatacggga tggaatcgga cgaaagggcg acgaacgcca aaatcctgag
     8821 accgtttgat tcttacggat acatacaaaa agacactttg tccaatgaag aaaaagaatt
     8881 aacttcgcgc cctgaaacac aaccacaacc ataaaaacat caagatggca ctattggaga
     8941 agattcgagt aaagatgggt atctttatta ccgtactcat agcagttgca ttattatctt
     9001 ttattattga ccccggcaca ttacagtcag ccctgtccat gttctcatct aaaaacgacg
     9061 tggggaagat gaacaggcag ggcataacat atatggaata cgccaaaaag ctggaaaaat
     9121 tcacaaacct gcagaaggcc atcaccggag gctcttccct ggatgaacaa agccaggaag
     9181 ctgtagagca gagtgcatgg caggccttcc tgaaagatct ggtgtacatg cctgccatac
     9241 aaaaagccgg tattagcctt ggcgatgaag agctttttga tatggtgcag ggacgtgata
     9301 tttccccggt tatcctgaac gaccccgttt tccagggaga agacggccag tttgaccgca
     9361 cgcggcttac catgtttgta cagaacgccc ggacggaacc gaaaggaatt gccgcattgt
     9421 actgggagtt tttacaggac aacatggaaa cggaaaggat ttttacaaaa tacagctccc
     9481 tgctgtccaa aagcaatatc gtgactcccg ttgaattgcg ccggcaaatg gaagaaaaca
     9541 acattacgtc caatgtaagc ttttcactta cccccgtatc gtttgccaga gataccacca
     9601 taaaagtcac atcacaggaa atacgccagt attacgaagc tcacaagcac cagtaccagc
     9661 aaatagccac gcgcgatatt gaatacgtgg tgttccccat cctgccctcg gcagaagatt
     9721 tcacactggc ccaggccaat atcgaaaaag tgtacgaaga gtttgccact tcagataacc
     9781 tgcgtcaatt ccttgcccgt aattcagaca aagccctgga cacctattac tataaaaaag
     9841 gagaattatc ctctgtttcc ccaaaactgg acagctttgc gttcaaagcc accatgcggg
     9901 atgtgcttcc tgtatacaaa gaggataact ttttccgggc agcaaggatt gcagacatca
     9961 aacaactccc cgattcggta tacgttcacc acatactgtt gcaaaaaaca aataaaagag
    10021 aggccactgc cctggccgac agtctgattg ccatactcaa ccgcacaccc ggccgttttg
    10081 ctgaactggc acaggagttc tcggcagaca ataatcccaa cgccggcccc ggtgaaatag
    10141 ggtggtttta ccaaaaccgc atagtccccg gaatggagtc ggtgtttgat gctcccctta
    10201 acaaacccta taccctggaa acacaatatg gccttcatat tgtcaaggtt tcggaacgca
    10261 cccgcctgca taaaaaggta cagcttgccg tgcttgtcaa ggaagccgtg gcaggaaagg
    10321 atacgtacca ggccatttat tcgatggcaa acagcttgtc cacggaaagc aaggacctgg
    10381 aagaattcaa ccgggtggcc atgcaaaaca acctgatgat cattcctgcc atgaatatca
    10441 cacaggatgc caaaaccatt tccggatatg aacatatgcg ggaactggtc cgctgggctt
    10501 tcgatgccaa atatggagcg gtttctcccg tgatatcggt ggacaataaa tatttctttg
    10561 tggcagccct taacggtatt catgaacagg gaacatccac cctgcaagag aaacaggccg
    10621 agatagaatc catcctcacc ctggaaaaga aacaggaaaa atcttaccgg cagatacgcg
    10681 aagaactggc cggccatact tcccttgaat catgggccga ggccactggc aggaccatat
    10741 ccacccagac cggactggca ttcggttcca cgcagcagac agacccgaaa ttcgtgggag
    10801 cagtgacagc cgccacagaa cagaccatgt acggaccttt caaaggcgag ataggcgtat
    10861 atgttttccg tgtcgatgaa cgtcttgacg gtgcctatta tacagaaaat gacgcaaagc
    10921 agaatgccgc atatctctcc ggcgtgcagt tacagatggt tccctttgtt ttgcaggaaa
    10981 ttgccaaagt ggaagataac agggcccggt tctattaacg gctgaacaga aagtgcatga
    11041 tgtccccgtc ctgcaccaca tagttcttgc cttccaccgc catctttccg gcttcacggc
    11101 aggcagcttc ggaaccatag tgcatgtaat ccttgaactt gatcacttct gcgcgaataa
    11161 aaccgtgttc aaaatcagaa tgtataagcc ctgctgcctg cggggctttt gttcctttga
    11221 ggtaagtcca ggctttgact tccatgggcc cggccgtaaa ataagtttcc aggtcgagta
    11281 acttgaatgc tgccttgatg agacgtgaaa cacccgattc ttccaggccc atggattcca
    11341 ggaaaatctg acgttcctcg aaggactcca gctctgcgat ttccgattct atgcgtgcgg
    11401 ccactatcag gtattgttca ttttccttct ccaggatacc tgtcacacga tttgtataat
    11461 ggttgcctgt cagggcagac ttttcgtcca cattgcagac atacagcacc ggtttattgg
    11521 tgagcaggaa cagttcccgg gccatgtgtt cttcctcctg attgaataat tgcacggacc
    11581 tggcgttttt tccctggatc aggacatcgc ggtagcggga aagcaaaccg gcaagcctgg
    11641 ctgcctgttt gtcccctcct gtaacagcct gtttctgtac ctttgccagt cggttctcca
    11701 ctgtttccag gtccttgagc tgcaattcgg tatctatgat ctccatatcc cttaccgggt
    11761 caaccgagcc gtccacatgg gccacattgg gattgtcaaa acaacgcagg acgtgcagaa
    11821 tggcatctgt ttccctgatg ttggaaagaa actggttccc aagcccctca cccttgctcg
    11881 ctccctttac cagcccggca atatccacaa aatccaccgt tgcaggcaca atacgtccgg
    11941 ggtggaccag ggatgccaat tgttccaggc ggggatccgg tacctgtgtg gagcccaggt
    12001 ttggctctat ggtacagaat gggaaattgg ccgcctgcgc cttgcccgat gaaacgcaat
    12061 tgaacagggt ggatttgcca acattgggaa gtcctacaat accacattta agtcccataa
    12121 agcaggtttt attgttttga agggcacaaa actaataact tttttacgat ttgtcctttt
    12181 tatcctcctg catgttcata acttgtgttc atgttacggt ttattttttg tttggtaatc
    12241 ttctggaatg tagaaaacta ctttgatccc tttgatgatc cgcttaccgg cgacaatgat
    12301 ttcaccactg ccggctcgta cacctggaca tggaacaggt ttttgaaaaa gcgcaatgat
    12361 attgtcaaaa cactcatagc ctgcggcgag caggaagatg gttcctgggt ggcgccctgc
    12421 atagtgggcc tgtgcgagat tgaaaacttt tttgtcctct ggcagcttgt cagcgacacc
    12481 ccgctttccc cgcttggata cggcatcatt cacagaaatt cccccgatcc ccgcggaata
    12541 gacgtggcgc tgctctaccg gaaagaacgt ttcagcccgg taaagacctg cttctacggc
    12601 atcccttcgg aagacgggac tttgctcaag acaagggaga tcctctatgt cagtggagtt
    12661 ttggacgggc gggacaccct tcattttctt gtgaaccact ggccatcccg gaggggagga
    12721 gacatggccg aggcaaacag gcaagctgcg gcccgacttc ttcagggcat tgtggacagc
    12781 attgccacag cttccccggg ggccaggatc gtggttatgg gcgatttcaa cgatcctccg
    12841 gaggccccgg tactgcgcag cctgtcgtcc gacagcctaa tcaatgtttc agccggcgac
    12901 acatacaaat acaaaggggt ctgggaatgc atggaccaat tcctggtaag tcccactgta
    12961 aacagggaac ttgaatatgt gaaagtggcg cgccttccgc atttgctgga gcgggaccag
    13021 gcacacctgg gacataagcc aaaacgcacc taccagggac cggcctacag gggcgggatc
    13081 agtgaccacc tgccgataac cttaaaattt attacctttg tttcttatgt catcccgaag
    13141 cgttaagaca ccactgatgg accagtattt tgccattaag gcagaatatc cggatgccat
    13201 gatgttgttc agggtgggtg atttttacga gactttcgga gaagatgcca tcaaggcatc
    13261 caaagcgctg gggatcgtgc ttaccaggcg cgccaatggg gcagcctcgt cggtggaact
    13321 ggcgggattc ccttaccatg cgcttgatac ataccttccc aagctggtac gtgccggata
    13381 caaagtggcc gtttgcgacc agctggaaga tcccaaactg accaagaaaa ttgtaaaaag
    13441 aggcatcaca gaggttgtga caccgggtct tgcctacaat gccagcctgc tggaatcttc
    13501 agagcataat tttctggcca gtctttatct ccaccaggaa ggaacttcag aatcggcagg
    13561 cattgccttt accgacattt ccaccggcac catccagatc gcacagggag acatggctta
    13621 tgccgagacc cttttatcac aatacgcacc cagggaaatc ctggtgcagc gcggaacaga
    13681 aaagaaatgg aaaccgctgg cagaccatca ggcgtatttt tccaccatgg atgactgggc
    13741 ttttgttcac gcagcggcag aggaaaaagt aaagacacac tttggggtgg agagcctgaa
    13801 aggattcggg gccgaaaatc ttcccctggc cgtatgtgcc gtaggagcca tgctctttta
    13861 cctggagatg aacaaatatt ccagctatgg ccacctgtgc accatgcagc gcattgacaa
    13921 ggagcaacat atgtggctgg ataaatacag tatccgcaac ctggaacttt ttccacgtcc
    13981 ggagaacaac cagaagggag ccagcctgat agaggtcata gacaagacca atacccccat
    14041 gggcgccagg ttactgcgac agtggctggt catgcccctc cgggacagga cggccataga
    14101 agagcgccac aatgtggtgt catctctgct ggaacaatgg gaactggccg gcaagctccg
    14161 caagctactt tcccaggtag gggatatgga acggcttatt tccagggcag ccgcaggcag
    14221 gatcaatccc cgcgaagcgg cccagctcag gaccgggttg aaccaggttt cccgcatcag
    14281 gaaactaaca gcagaacttc cgcaggaaaa cccggtatat acccttgctt ccaccctgga
    14341 cgattgcacc gggctggtac agacactgca gcaaatgctt gttcccatgc cccccgcaca
    14401 actgggaaaa ggaaccgtga ttgcaccggg cacggacacc gccctggacg aattgcgcca
    14461 cattctctcc cacaacaagg aatacctgca ggaaatgcag cagcaggccg ctgcgcagac
    14521 gggcatcact tccctcaaga tcggctttaa caatgtgttc ggctattata tcgaagtccg
    14581 caacacccat aaagacaagg tgcccccgtc gtggattcgc aaacaaacgc ttgtgagcgc
    14641 tgaacgctac atcacggaag atctcaaatc ctatgaggaa aggatccttg gagcagaaga
    14701 aaaaatagcg caactggagc aggacctgtt caaccaggtg gtcgacgcca ttcgtgccac
    14761 catacctccc atccaggaaa atgcgcgcat tctggcacgt cttgactgcc ttctgggttt
    14821 tgccatactg gccagggaac aaaaatacga aaggccacga atgcaggatg acgacgtgat
    14881 tgacatccgc cagggacgcc acccggtgct ggaaacgctc atgctgcccg gagaagccta
    14941 tgttcctaac gatgtatacc tggacacggg acagcagcag atcatcatcc tgaccggacc
    15001 caatatggcc ggaaagtccg ccctgttgcg ccagacaggg ctcatcatgc tgttggcaca
    15061 gataggttct ttcgttcctg tcagggaagc caccctgggg atcatagata aactgttcac
    15121 ccgggtggga gcttcggata acattgcccg cagggaatcc acctttatgg tagagatgct
    15181 ggaatctgcc gcaattctca ataacgtgac cgaccgttcc ctggtgctct ttgacgagat
    15241 aggacgcggg accagcacct acgacgggat ttcgatagca tgggccattg tggaatacct
    15301 gcacgaaaag cccggggcaa gtgcccggac actgtttgcc acgcattatc atgagctcaa
    15361 ccagatggca gagcaatacc cacgggttaa aaattttcac atagccgtga aagaacgggg
    15421 acaaaaggta ttatttttgc gtaaaatgat ttccggagga gtggaacaca gttttggcat
    15481 ccatgtggct accatggccg gtatgccccg cagggtgatc ctgacggcac aggaagtgct
    15541 caagagaatg gaagccggta aagaacaacc tccttccccg gaaagaccca ggcaactgtc
    15601 tttcttccag ctggaagatc ccctgttgca ggctctgaaa agagatattg aatccataga
    15661 tttgaacaga ttaacacccc tggaagcatt tgatgccttg cgggaattga aaagaaaaat
    15721 aggacaaaag gaatgaaaaa gatcttcaaa agcatcaaca tcttctttaa actgctgggt
    15781 gaaagctttg tgatgtcata cgactctgta aaaggagaca aattcagggc ctttctctcc
    15841 ctgctgggag ttaccatcgg gatattttcc atagtagccg tcttcaccgc catcgaggcc
    15901 ctggagaaaa atgtccggaa catgttttcc gcgctgggaa ccgatgtggt ttacatagac
    15961 aagttttcct gggaagcttt tacggaagaa ggaggtttca aatggtggga atacagacaa
    16021 cgccccaata acacctacga agaattcatt ttcctgcaaa agaacctgaa ccacgcagac
    16081 atggttgccc tgtcggtcta caccaccagc aatgtgggat atgagagaaa cgtcatccgc
    16141 aacgcatcca tccagggggt cacgtatgac tggaacaaga tttcttattt cgagttaagt
    16201 gaaggccgtt atttcacctt cgccgaggca gccgaaaaca tacccctggt ggtgattgga
    16261 cagaacgtgg ccgatgaact ttttccgccc ggcatctctc cgaccaacac caacatcaag
    16321 atcgggggct ttaccgcccg cgtcattggc gttatcaaga aaaaaggcga gtccatgttt
    16381 gaccccatca cgtatgacga tgcggtcatc gtccccctgc tttttatccg caacttcctg
    16441 gatttgagga acacatcccc cagcatcgtg gtacacagga atccggaggc cgatgaagaa
    16501 gagtttatgg ccgagttgcg catcgccatg cgcgctatac gccgcctgaa gcccatacag
    16561 aaagacaact tcgccctgaa caaaatgtcg ttcctggacg atgccctgtc acaacttttt
    16621 aaagtcattt cgctggccgg ctgggtgata gggggctttt ccattctcat aggtgccttt
    16681 ggtgtggcca acatcatgtt tgtttctgtt aaggaaagaa cacatatcat tggcatcgaa
    16741 aaagccatgg gggcaaaaag ctcctttgtg ctggcccagt tcctttttga agctgtcctg
    16801 ctggcgatcc tgggtggtat gatagggatc ctggtcgtat ggggcctgac gattttcgtc
    16861 acccggcagt atgattttgt catgaccatg tccatgggca acataatgcg cggggtgctg
    16921 atatcagggc tggtaggcat tgtggcaggc ttcataccct cgctccaggc agccaggatg
    16981 gaccccgtgg aagctatgaa ccagcattga gaaaggcggc cacctgctgc gttaaagcca
    17041 cgaattcctc tacacccagt tgctctggtc tcagtcccga gaaaggtgaa gcaaagccct
    17101 ttttctcacc cagtaacggt ttcaggctgt tatgaatggt tttccgccgc tggttgaacg
    17161 tggttttgac cactttgcgg aacaattctt cagggcagtc cagggcagtt ctcctgttac
    17221 gcaccaggcg gatcacaccc gactgcacct tgggaggagg ggagaaagcg ccggcaggca
    17281 cggtaaacag atattctgca tcataccagg cttgcagcag gacactcagg ataccatatg
    17341 tctttgagcc cggaggggca cagaaccttt cggccacctc tttctgcagc atgcccgtca
    17401 caagggggat ggaatcccgc cattccagga ccctgataaa tatctgtgaa gagatgttgt
    17461 aagggaaatt ccctatcagg agaaaccggg gagggaaaac cgaacgtgcc gccgtgagga
    17521 aatcaccctc aatgagccgg cttcccaggc cggggtattt tttttgaagg aaacccaccg
    17581 attccctgtc cagttctatg gcatggtaat caaggcaggg tctttccagc agaaaatccg
    17641 taagcacgcc ggtgcccgga cccacttcca caaccgggat cccggcgccg gagcgcattt
    17701 ccttttccgg ccacagtccg tccactatcc gggcggccac cttcaggtcg ttgagaaaat
    17761 gttgtccgag tgatttttta ggccttactt ccatgataat acaaaggtag ttttttatac
    17821 ctttacggaa atctaacact ttaatctatg aaaagaactg tcataattat ggccgcgctg
    17881 ctggtcctgg tatcctgttc cggctacagg acgcataaag cggatatccg cctggatgaa
    17941 ctcaggcagg aactcaatgc tgtgggactg tcagttgccg taatatccga caaccaggtg
    18001 gtgtatacag gggcaaccgg tttcaggaac atggaaacca gggaaccact cagctcctcc
    18061 gacctgatgc gtatagcctc catatcaaaa agctttacgg caaccgccat catgcagctt
    18121 tatgaggcag gctgttttga cctggacgac gacataggaa ccgcactggg ttttccggta
    18181 aggaatcccc attatccgga catacccatc acttatagga tgttgctgtc ccatacatca
    18241 agcctgagcg acagtgcggg ctatttctcc ctggacgtgc tccgtcccga catcaccaaa
    18301 gacgtgtccc cgtccttcaa tgactatgct cccggcacag cataccagta ttgcaacctg
    18361 ggtttcaaca ccctgggaat ccttgtggag atccattcag gggaacggtt tgaccaatac
    18421 ctctggtata acgtgctgga acccctgggc gtttacgcct cctataatgt gaatgacctg
    18481 gacaccaccc gttttgccac catttattcc tttgaggatg gtgagtttgt accggcaccc
    18541 caggcataca accccaggaa agaagatatt gaaaattatg tgatgggttc ttccgccgta
    18601 gttttctctc ccacaggagg gctcaagatc agcgcaccag acctggcgcg acaggcgttg
    18661 gtccagatga atgacgggac cctggacggc agggaagtgc tcacacggga aagcgttgca
    18721 ctcatgcaaa caccccaaag cccaggaggc cgcgatacca tggccgaagg cctaggctat
    18781 ggatttgccc tgcaaaagac ggataaactg attgaagggg aaatcatgac aggacatacc
    18841 ggaagcgcct acgggcttta ctcggccatg ttctttgaaa aggataagaa attcggcttc
    18901 gtggtcctgt gcaatggctg cgacacgtca atacccaaca gggataacgg attcatggcc
    18961 gtgcagacag acgtgatcaa cgccctgtac gacatcttca tccggtaagg gcctgtgccc
    19021 tttacttaag gcccagttcc tttttttgca aggcatcaat tccggcggga gcatccagca
    19081 tgacatccct gccggaattg tttttcggga atgcaatgaa atcgcgaata ctctccgatc
    19141 cgccaaagag cgaacacaag cggtcaaatc cgaaggcaat accgccatgc gggggcgcac
    19201 cgtagcggaa cgcatcaatg atgcaggaaa acctggcatt tacctcctct tccgtcatgc
    19261 ccaatatccg gaacatcctt tcctgcatgc gggcatcgtg tatccttatg gaccccccgc
    19321 caatttcagt gccattcagc acgaagtcat atgcgttggc attcacctgt tccaagtcac
    19381 ggggatcgtc gctgtagaac ttatcaaggt cctccggctt gggggctgtg aatggatggt
    19441 gtttggcata gaaacgttgt gtttcctcgt cccattccaa taaggggaaa tcgtacaccc
    19501 acaaaggggc aaaaacatct ttgtttctca atcccagccg gctgcccatt tccaggcgca
    19561 agcttcccaa tgcttccagg gtttttgttt ccgggccgct cattaccagc aacaggtctc
    19621 cccctgttgc gcctgtctgt tgcgtccagc gggccagcgt ctgctggtca aaatatttat
    19681 ccacggaaga tttataggcc accgagccgt caccttctgc ctggcaacgc acatatacca
    19741 acccggaacc gcctacctgc ggccgtttta cccattctgt aagttcgtcc agctgctttc
    19801 tggtataccc ggctgccccc ttgacgcaaa tggcccccac ataggcggcg ttcctgaaga
    19861 cgctgaattc cgtatccctt gtaaaggtgg taagatcgtt tatcaacatg tcaaaccgaa
    19921 tgtcgggctt gtcgcttccg tatttttcca tggctgtctt ataggacatg cggggcaggg
    19981 gagctgtgaa atccaggttg tgcacctttt tgaaaagatg cagtatcaac ccttcaaaca
    20041 cagaaaggac atcgtcccgc tccacaaaag acatttcgca gtctatttgt gtgaattccg
    20101 gttgccggtc tgccctcaga tcttcatcgc ggaaacagcg cacgatctgg taatacttgt
    20161 cataaccagc tatcatgagc agctgcttta attgttgcgg gctctggggc aaggcataaa
    20221 actgcccctg gttcatgcgc gagggcacca caaaatcacg ggccccttcc ggggtggatt
    20281 tgatcaggaa aggtgtctcg atttccagga aaccgcaatt gtccaaataa ccgcgtgttt
    20341 cctgtgcgat tttgtgacgc agtatcatgg ccttttgcaa cgggggcctt cgcaggtcca
    20401 ggtagcggta acgggcacgc agttcttccc cgccatcgga gatttcctca atggtaaagg
    20461 gcggtacctg cgacgggctc agcacactta tcccgccgag gtcaatttca atttccccgg
    20521 tggggatttt caggtttttg ctgcttcgtt ccacaacaat cccctcggcc gagaccacaa
    20581 actcacgccc cagcgattcg gcggtttcct ttaccttgac cggggaatct tctttgacta
    20641 ccagctgggt gattccatac cggtcccgca ggtcaatgaa agtcattcct cccagttttc
    20701 ttatccgttg tatccacccg gccagtgtaa ccctctggcc ggcgcaagac aggtcaagtt
    20761 ctccgcatgt gtgtgtcctg tacatttcca aacattttat ggtcacaaag gtaatgagaa
    20821 aatggctacc tttgttttga tatgaaaaac tatttggacc tgttacaata catactggat
    20881 aacggaaccg acaaggagga caggaccggt accggcacca ggagcgtttt cggataccag
    20941 atgcgttgtc ccctggcaga gggattcccc ctgctcacca ccaaaaaagt acacctcaaa
    21001 tccatcattt atgaattgtt atggttcctg cgtggtgaca ccaacattgc atacctcaac
    21061 cggaatgggg tgaccatctg ggatgagtgg gcagacgcgc ggggagacct cggacctgtg
    21121 tacgggaagc aatggcgttc ctgggaaacg gcagacggac aagtcataga ccagatagca
    21181 gcccttattg agggactcag gtcaaatccc aacggcaggc gccacattgt cagtgcctgg
    21241 aacgtaggac agatctcccg catggcgctg ccgccctgcc acgtcctgtt ccagttctat
    21301 gtcgcggacg ggaaactctc ctgccagcta taccagcgca gtgccgatgt tttcctgggc
    21361 gtccccttca atattgcctc ttattccctg cttaccctga tggtggcaca ggtgtgcggc
    21421 ttccggccgg gagattatat ccatactttc ggggatgcgc acatatacag gaaccatatg
    21481 gaacaggtgc acctgcagct ttcacgcacc ccgcgcccgc tgccgcacat gatactgaac
    21541 ccggatcgtc acgatatttt cgggtttgaa tactccgatt tcacccttca ggattacgat
    21601 ccccatcccc tgatcaaagc tcctgttgcc gtatgaaaag tgaacatcca tccaaaccag
    21661 gcattctttc ccttatagcc gtactgggac catcagggga actcggcgtt cgcaacgggt
    21721 tgctgtggca tatttccgaa gacctgaaac atttcaagtc cctgaccatg ggacataccg
    21781 tcatcatggg ccgcaggact tttgaatcgc tgggcaaaca gctgccaggg cggaccaaca
    21841 ttgtgctttc acgttcagga aagaaactgg aagaaattct ggaggacgta aaagatcagc
    21901 cggaagtgtt tatcatcggg ggagcccagg tctaccgcca ggccatacac ctggcagaca
    21961 ggctttatat aacccatgta caggccccgt tgccatccgg aggggccgat gtgttttttc
    22021 cgcccataga tccccggcaa tggcaggaag tatcgcggca ggactttgtc aggggagctg
    22081 ctttcccgta tcctttttcc ttcgtaacat acgaacgcag gaaaacacaa cagcagccgc
    22141 cagtgtaagc aacagcccaa cagagctgat gcgtgaaacc aggattcccg ttgtgtagga
    22201 agcaggcagg taagtaaagc gtatgccgtg ctccccggca ggcacaagca gggtgcgggt
    22261 gatataattg gaccgcagga tgggcacctc ttccccgtct atccaggcct tccatccttt
    22321 cggatagaaa acctccccga ataccacaat cccgtctttt tccatggatg cgttgtattc
    22381 cagcgtattg ggagaatagg atgccagtgt tacagtcgaa cctgacggac tggcggcctg
    22441 tacccattct tccggggcat cacgcatcac cgctgttgtc cgcaggtcgg cacgggaaag
    22501 catttccagt tcttccaggt ttgtctccac gggcaggata gaagacacca cccaggcgtt
    22561 tcccagggca tgcgggttga caaccggagc agcgtcaccg gacaggatga cataccgtgt
    22621 gttgagcaga ttcatgaccg gggtgcttgt ctgttccccc tggtatgcaa atatttccga
    22681 ggcgtcttgc agcgtctgac actgtctgat cttttccatg aaatactgca tttccggtaa
    22741 caggtggcgg tctatcacat cctgataccg ctgcagtttg gccgcattgt atccgcctat
    22801 ggatttgtgg tgataactgg gacgggattc agagaaagga cttcccgtaa gatccagtac
    22861 gcgatagtac atgtccggat ctccctttat gagtttatcc acgggtcttt ctgcaaactg
    22921 gttgctgtaa ttgctgcggg tgacaaaatg tgaatcgttc aggtaacgtc tggctgcagg
    22981 caggtaatcc accaggagca ggatgcccag ggcggcgaca gcccataccg ttctcatttt
    23041 tttatggaca gccagccaga tgacaagtgc cgctcccgct atcaggaaag cagaacggaa
    23101 ggcatcctgg cgcaataacc ctttccgatc agccaccagc gctgggagca gttctccggg
    23161 gagcgaggca tcggaaggcc ctgtaaaaga acccgccagg gaaggagcca gggcaaaaac
    23221 aagacatagg ccggcggtaa tgcccaggct ccagtatagg gcccgggtgg ttttttcctg
    23281 cggatattga ttatccagaa gattccatac agcatatata cccagcgcag gcaccaccat
    23341 ctgccagatt accaggatca tgcttacggt acggaactta tcgtacaagg gaacgtaccg
    23401 gaaaaacaac accgaaaacc attccaggtg gtatccccag gatagcagca atgccagcag
    23461 cgaaacgccc gcaagggccc atttcagggg ccctttgacc aggatcagcc ccagtacaaa
    23521 aaagaagatc atgatggctc ccatgtacat gggcccggcc gtgaagggtt gttctcccca
    23581 gtacaggggc atgttggaaa taatgctcct ggcgttgtat cccatgcccg aaagtttttc
    23641 ataggagtgc gaatcctggt ccagtgcccc gacagaggcc cctcccctga aatcggggat
    23701 catcatgttg aaagattcca tttgggaata gctccactgg gtggcgtatt caacatccag
    23761 tcccttgcgc ggggcctctc cttccgggtt ttccagttca gatccgccac gcatggaata
    23821 ttgcgtgtat tcatacatgg gaagcaggtg atttgccgag gtggccaggc ccagcaatcc
    23881 ggccgcgatc agcaacaccg tggtgttgac aaaacgcggc aaccgccgcc tgatgatggc
    23941 ttctgcaagt aaaccgccaa aacaaaatac catgatgaat cccaggtaat aagtgatctg
    24001 gggatgattg gcagccacct ggaaactcac ggtcagggca aaaaacaatg ctccccacaa
    24061 ggcatttttc cggtatgcaa acacgagtga agccagcacc cagggcatat aggcgatggc
    24121 tatcatcttg gtcacatgtc cggcctgtat gatctgcatg ttgtaagcgc agaaggttat
    24181 ggcaatggct cctcccatgg ccagccaccc gttgaccccc agggcaagga acagcagaaa
    24241 accacctaca agtgagagga agagatacga tgccggcgcc ttgccggctt ccagaacccg
    24301 gctgatcagc cgggtataat tgccccgggt gactttattg atggtaatgg taggcatgcc
    24361 actgaacatg gagttggtcc agagggcctc gtcctcatgt gtctggttgt aatcttttgc
    24421 ctcctttgcc atggcctgcc aagccgaaat atctccctgg ttgaggactt ttccctggaa
    24481 aatctgggga acaaaaccat aggataacac caggaaaaaa acggcagcta ccggatatac
    24541 gatccatttt ttaacaggaa tcttctgcat agttctctta tttactaaca aaggtaaaaa
    24601 gaaaggacgt atatttgtct aaaaccgact ctttatgaaa cgtgacgaat tttcaggatt
    24661 ttttggagtt ctggtagcca ctgtgggttc tgctgtggga ctgggaaacc tttggcgctt
    24721 cccttatctg gtagggcaaa atggaggtgc cgccttcatt ctcatatacc ttgcctttgt
    24781 tctggtcatc tgtctgccca tcatgatctc tgaattcatt atcggccgcc ggacacaggc
    24841 caacacggta acagccttcc aggtgctggc tccccacaca ccatggagcg ttataggaat
    24901 tgtcggggtt gtagccgctt tctgcatcat ggctttttat accgttgtgg gaggctggac
    24961 actggattac attttccgct ccgtgatcca cctcttttcc aaaccagatg catcggccat
    25021 cgaaagccag tttgcctcac tcgttacatc cccggtccgg cccattgtat ggacaattgt
    25081 gtttatactg ctcagcgccc tgatagtcat gtccggcatc aagaaaggga ttgagaaata
    25141 ttccaaggtc atgatgccat tcttgttttt aatggttgtg atcctgatga ttcgttccat
    25201 taccctgccg ggggcagaaa aagggatttc attccttgta aagccggatt tctccaaaat
    25261 cacaacgcag gcagtactgg ccgccctggg tcaggccttt ttctcgctca gcctgggaat
    25321 gggttgcatg atcacctatg gatcatacat cccccgaagg gaaaacctga acaaactggc
    25381 ctttaccaca gccatgtcag atactctgtt cgccatcctg gcgggactgg ccatcatgcc
    25441 ggccgtattc tctttcggga tatctccaca ggaaggaccc ggtctcgttt tcatagtcct
    25501 gcccagaatc tttgcccaga tcccgctggg taatattttc gctgtgatgt tttacctgat
    25561 cctgttcatt gccgccattt cttcggccat atcattgctg gaggtggtga cggcatatat
    25621 aacagaaagg ctgaaaatga aaaggaaggc agcggtagcc atcatctctg cactggtagc
    25681 agtgtgtgcc tctttcagct ccctgtcaca gggagtgatg caggatgtga aaattcttgg
    25741 caagaccata tttgatttct tcgattatct ctctgccaac atccttctcc cgctgggggg
    25801 actctccatt gtactcttcg tggggtggta cctgaagaaa accgacatct atgatgaatt
    25861 tacaaatcag ggcacccata agggtaccct gttccgcgtc tattattttc tcatacgtta
    25921 tgtggccccc attgcgatag cggccatatt cgtggacctt atcgtacggt gatacccagg
    25981 tgccagttcc cgttataacc caggtctttc agcgaggtta ccgtattctc ttcttcttct
    26041 gaggtcagga gcaacaggtt cccgccctca tgggcaggcc cttccccgtc tgtcatcaat
    26101 acgggctgtg tccccttttg cgcgatgatc atgttaagga taaccccgtc accatcctgg
    26161 atctggacga tataattttc tgtaacaaag gttttcacca aatcccattt gccggtagcc
    26221 ttgatggcgt caatgaaata tttcttaagg ttcattgtgc tttgtgacaa gagattcgtt
    26281 tgcgcgacag aggcatcctg aaccattatg atacgccggg aaagtgtttc tataaaattc
    26341 acatactgcg tgggatcggt aggctgctgg ggattgcctt cttcatcgac aagcgaaatg
    26401 aagatctcat atatttctgt cagctgattt tcatcttttg ccattatgac aatggaagaa
    26461 gttgtatcca tggggcagag catgatgttt tccagtattt tccctttcca tgttttaaca
    26521 aaatcgggag ccatctgtac cagcaccatg acattgtcat ctgccaccgg cgaggagaaa
    26581 ggcctgatcc ccgcattggg cctgatcaac gataatgtgg aggaggtcac ggacaacaaa
    26641 ggaatatcct ttacctcaat gttggaaaca ccctcgggaa tggttagcat ggtatagaag
    26701 tccttgacca ggccataccc gttaacgcgg acaccgtagg tgccggggac cacgtttttg
    26761 atttgtacgc tgcccccagc ggcgctggtc accttgtaat cataatcatc tgcagcagca
    26821 ggccttatag aggtgctccg catggcaggc acttgcaggg tcccgttctt cagggcctcc
    26881 aggttgttcc tgatctgcga ggcatagggt ttcatcaagg aagtcagcca ctcgccggca
    26941 gattgcagga cttcctcctg tgtacggcct tttattacgg gcagggcttt acccgccgta
    27001 tgcacgtaag ccggtgaggc cgatctcttc tccgaggaaa ggagtttcag atatacatcg
    27061 gcataatcca cggaagcacc ggtgttgtca accaatacca tattcacttc gttataccgc
    27121 ggtgtggtca ccgtgatctc ctccccgtat gccgtacctg cctggctggt tgcataggct
    27181 ttgattgtat aggccgtgtt ttcatgaatg ttgccggtaa aaattatgga gaaaattccc
    27241 gttccgtccc ctgcctggcg ggttgtggtg ttgaggacaa aacccctttc agtcaccgca
    27301 tggctgcctt gtttgagtac ctcggcagaa acacttattt tgttgtataa cagttcagtt
    27361 acagtcacct tgcccacggt gggaagcacc ccttccagtt ccaggaaaac cccgttctgc
    27421 tgggtgcgga tgtagtggat ttcggtattt gttttggagc tatcccactt gtccgacgtc
    27481 caggacgggt ggtttatgat gacagccggg accggtttag tcagtttatt gccatagaca
    27541 cgcagttggg acgatacggc cggaagattt ccccacgagg cagggatccc accggtgaaa
    27601 ttgcattcat gcaaatggat gttgaaacgt gtctgggtgg tggtcatgtt gcccagccct
    27661 tcgggcagtg acccggtcag gttggggttg tcgttcatca ggaaagtggc caggtttttc
    27721 catccggtaa agatctcgga gggaatgggc cccgagattt tggtgttgaa catcccaaag
    27781 ttgttcagtg ccgtcattcc gccccattct ttgggaatgg ttcccgagat ctggttattg
    27841 ttcaattgca ggttggtcag cttgttcaga tttcccattt ccgcgggcag cggaccctcc
    27901 agcccggtag tcaccgccat cagcgccgta aggtttacca ggtttcccag ttccgctggc
    27961 agcggcccgg tcaggttggg attgctaccc aggttgagtg ttttcagcgc cgtcagttcc
    28021 cctatttgtg ggggtaactg ccccttcagc ccgaaaccgc tcaggctcag accggttata
    28081 gcctcatccg taacagtgac cccttcccat gtgccgagtt ccgcgtctgt gttccagttt
    28141 gtacccttgg tccagttggg tccgtccagg gcgttgtaaa gcgccatcag acagtcacgc
    28201 tgggcatcgg gaacctgttc cagttccagg aaaatcccgt tctgctgggt acggatgaag
    28261 tgggtgccgg catctttcca tttatcccac ttgtccgtag tccaggacgg gtggttttgt
    28321 atggacaggg gtaccggctc tgtcagtttg ttcccgtaaa ggtgcagttg tttggacacc
    28381 gtgggcatac tgccccattc ttccgggatt cctccttcaa aattgcaatt gtacatctgc
    28441 acactgaaga gcgtgttgtc ggtttccatc tgtcccagtt ttaagggcag ggagccggta
    28501 aggttggggt tggagtgcag aaggattgtc cctatgtgtt tccagttctc gaatatcgta
    28561 ttggggaggg ggctggagat tttggtattg aacatcccga aattctttaa agccgccatg
    28621 tctccccatt ccgaggggat agtaccggtg atattgttat tgttcagctg caggttcgta
    28681 agttttttca ggttacccat ttccttgggc aacggacctt ccagtcccgt ggtaacggcc
    28741 agcagcgcat ccaggtttgc caggtcaccc agcgaggaag gcagcggccc tgtcaaatcg
    28801 gggtttgaac ttaaggtcaa ctttgtaagg tcggtcaatg tcccgatacc cgcaggaagt
    28861 tggccattca atccaaaccc actcaggttc agttgtatga tcttaccttc ctccatggtt
    28921 accccggccc attgatcaat aggctcttca gtcttccagt tggtcttttt ctcggccacc
    28981 cagttattcc cgtccatgtt gtcatacagc tgcaccagaa gggtcctcag cgcattctct
    29041 ttctgattga aggtgaaagt ctgggtctgt gcaattccgg ctttgtccac aacagatacc
    29101 tttccagagc ggggggaccc cgtgttggga gttatcagaa actgaagcgt tgcagtagac
    29161 aatgccttgg ttcccgtata ctggatccac gatttggcgg cctcttctat ctctaccgtg
    29221 tactccgtat tgtactgcaa ggtgagggag taagtacccc cggaagcctc tacagtatta
    29281 tctccattaa caatgagggt attgcgcgca ctttgtgtaa tggtcaccga ggcctgcatg
    29341 gtgccgcttt tgatggtcag cattcctgtc tggtccgacg acgaggtaga agcagctgct
    29401 gtcaattgca aggtggcatc gcctgccccc gaagcgggag aaatagaaaa acccttgccc
    29461 gaaacagatg cagtccagga catactgttg gtagacaggc tgatggcctg cgttcctcct
    29521 tcctgcggga agataacgct cacggggctg gcttctatgt aaggaacttc cggtttgcag
    29581 ccggaaataa acagcagcgt tgctgctata accaataaac ttgtgatctt tttcatgttt
    29641 cctagacgct aattgttact atttggttgc ttaggctaat aaagatgtca atttaccggc
    29701 aagatcctta aaggcctgac cggcagcatt tttatcgttt gccacgggta tgcctgcatc
    29761 gccgcgttca cagacactct ggacaatggg gatttgtgcc agtagtggca tattgtattt
    29821 tttggccagt tcttctgctc cgccttttcc gaaaatgaaa tattttttat caggcaattc
    29881 ttcaggggta aaccaggcca tgttttccac cagacccaac acccgcttgt tcagatcggg
    29941 gtgcatgaac atattcaggc atttttccac atcggtgacc gacacctgtt ggggtgtggt
    30001 aaccacaata cccccggtca ggggaatgtc ctgcatcagg ctcaggtgga tatctcccgt
    30061 tcccggagga aggtctataa gcaaaaagtc cagcacgccc cattttgtct gttgaagcaa
    30121 ttgcttcaga gcattgctgg ccatgggtcc ccgccagatc agggcctgtc ccggccgggc
    30181 aaaatatcct atggacatcc atttgacccc gaatttttca acgggctcaa aaacctgcac
    30241 attgtcatct atggcatcta cctgtggctg catttcctgc gtaccggtga gtatgggcac
    30301 cgagggtccg tatatatcgg catcggccag ccccacctta tatccttcca gggccagggc
    30361 cactgccagg ttgaccgcca ccgtcgattt acccacaccg cccttgccgc tggctatggc
    30421 tatgacatgt ttcaccccgt tcatcccgtc aattttgacg gattcgtttt catccctcac
    30481 ttttttcctg gcaggttgta ccggcttcat ggccgcataa tcgatctttc ccatgatttt
    30541 attttcttat atccatttct ttcagcaaat atcctgcctc accgtattta tccatgataa
    30601 aaagaacgta ccggatgtcc acggaaatac aacgctgcag ggcgggttca aagatcacgt
    30661 ccccgctcat ggattcccag ttgccgtcaa aggccaggcc aatcagttct ccgtcagcgt
    30721 tcagcacagg agatccggag ttgccgccgg taatgtctgt gttggcaata aaaccggtta
    30781 caatttcctg acccggcagg gcatattgtc cgtaatcctt atccctgtaa agttgtttca
    30841 gtttttcggg caccacgaat tcccagttat cggggtcttc tttttccata accccggtca
    30901 acgtggtgta gtaatcatag attacgccat ctttgggttt gtaattcaac acattgccat
    30961 aagtgagacg catggaggaa ttggcgtcgg gatacatggc cttgcctttg ttcatttcca
    31021 tcatgccggc aatgaagttc cggtgtccac gctcgtattg caggaaacag ggagcaatga
    31081 cggcctgaca ttctgcgttt ctggattcca ccgattgtcc cagcagcact gcgggatcag
    31141 aggcaatctc ctgcagggcg gcctcacggg tgttttcggc agacaaacat tctatcagag
    31201 aattgaccct ttcggcagag acaaaatggc ttttctcaaa aagatgttcc acgtaagcgt
    31261 ctatattgcc agagaactca ttttcaataa tcccgtaaac agaaggataa tacgctttgt
    31321 ccaccttcag ggcaaaatgt ttaa
//
LOCUS       DAOSOF010000003        30876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1493_length_30876_cov_4.780474, whole genome shotgun sequence.
ACCESSION   DAOSOF010000003 DAOSOF010000000
VERSION     DAOSOF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30876
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_30876_cov_4.780474"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLM68_00335"
     CDS             complement(<1..925)
                     /locus_tag="PLM68_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOT53851.1"
                     /translation="MMRLLVFLFSSLLIQVSAFAQQEGAVTFDKTVHDFGDFGINDGP
                     KTHTFTLTNNSAKAIVIQTVISSCGCTVPEWTKEPLQPAAKGTITVTYSNDQGPYPFD
                     KMMSVYISGFPEPFILRIRGVVHQKKFTLEDTHPVVMGPLRLRNRELELGQIRQGMVK
                     TDSVQVINTGKLPVFLKFESPDKNLQVSAMPSRLNPAESGMIVYSIDTRKQEDWGNVL
                     YTCQVVINDRRDPLHVLTVKGSIKMNTSGMTRADKEMAPIPQMDKSAVEFPLTPAGSE
                     IKTNFSLSNKGKSPLVIHKIDLSHAGIKVTMD"
     gene            1009..1176
                     /locus_tag="PLM68_00340"
     CDS             1009..1176
                     /locus_tag="PLM68_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT53852.1"
                     /translation="MAHKITENCIACGTCIDECPVEAISPGDIYVINPDECIDCGACA
                     AVCPSGAIEGE"
     gene            1332..4610
                     /gene="secA"
                     /locus_tag="PLM68_00345"
     CDS             1332..4610
                     /gene="secA"
                     /locus_tag="PLM68_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT53853.1"
                     /translation="MASFINVIGKIFGSKADRDLKELNPYVERIKKEYDRLDKLDHDD
                     LRAETQKIRQIILDHIAEDEKQKVALREQLEDMEIEVEKKEALATEVDKLTKKIDEKI
                     EEVLDSVLPQVFAIMKSTARRFFENKTLTVTASEFDRELSTSRDHVEIRGDKAIWHNS
                     WMAGGNRITWDMVHYDVQLIGGVVLHKGKIAEMATGEGKTLVATLPVFLNALAGKGVH
                     VVTVNDYLSRRDSEWMGPLYEFHGLRVDCLDKHQPNSDERRKAYRADITFGTNNEFGF
                     DYLRDNMAVNSDDLVQRKHHFAIVDEVDSVLVDDARTPLIISGPVAKGDDQQFNEFRP
                     IVENLYQAQRVLVQQFLADARKLIQDGDEENGGKLLLKAFKGLPKYNPLIKYLSEPGI
                     KQLLQKTENFYMQDNNKQMYQITDDLYFVIEEQQKSVNLTEKGHDLIAKKVSDATFFI
                     LPDMGTEISELEKKNITAEEKAAAKDALLNDYAIKSERVHTVNQLLKAYAMFDKDVEY
                     IVVDNKIKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMY
                     HKLAGMTGTAETEAGEFWSIYKLDVVVIPTNRPVIRKDEEDLIYKTKREKYNAVIDKI
                     DQLTKAGRPVLVGTTSVEVSELLSRMLKMRGVKHNVLNAKQHAREAEIVAEAGRAGAV
                     TIATNMAGRGTDIKLREGVREAGGLAIVGTERHDSRRVDRQLRGRAGRQGDPGSSVFF
                     ISLEDDLMRLFGSGRIAAVVDKMGMKEGEVLQHSMLSSSIERAQKKVEENNFGIRKRL
                     LEYDDVMNSQREVIYTRRRNALYGERVDVDVANMMSDYAESFMQRMDGVTYDDLRFEL
                     IRQISIEPGFDGEVFRKASTPEMAGLLAEQIQTTYNRRAEALIAQAWPIIKNIYQTQR
                     ATYENVVVPVSDGKRIYQVIVNIQKAYESKGKELTRSINKTINLVVIDEFWKEHLRDM
                     DDLKQSVQSATYEQKDPLLIYKFESFNLFSEMLEKIAREVLSFLMKAHIPLRQDQDRL
                     DKAGSERKRTDLSNTQTSRPDLLTQSVEPRSNAPVHVEKQVGRNDPCPCGSGKKFKQC
                     HGKN"
     gene            4629..6005
                     /gene="lpdA"
                     /locus_tag="PLM68_00350"
     CDS             4629..6005
                     /gene="lpdA"
                     /locus_tag="PLM68_00350"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT53854.1"
                     /translation="MLYDLIILGSGPAGYTAAVRASQLGFKTAVLERGPLGGVCLNWG
                     CIPTKALLKSIEVLNLSKSAQSYGVKLAPGAEPDLAAIVSRSREVSAQMSKGIEFLFN
                     KYKVEVIHGRGSVLGPGKVSAGDKILETKRILIATGAKPNSLHGASTDGKKIIAYRQA
                     MVPETIPATIAVIGSGAIGTELAYFYHSLGSKVTIIEYMDQLVPTEDADVAALLSRSF
                     RKAGIQVMTSARVKSADKSVGGCRLEVETKKGTEFIDADVVLSATGVVPNTSNLGLKE
                     AGVKMERERIIVDKNFMTSVPGIYAAGDVLPTPALAHLASAEALCCVERMAGLDVPDV
                     DYANIPGCIYATPEIASVGLREKDAKVKGIEVSIGKFPFTASGKAATAGEREGFVKLI
                     FNAQDSRLAGAHIIGAHATEMIGGLVACIGMKIKDMDLARTVFPHPTMSEGIMEAAAL
                     ACGHCVNL"
     gene            6017..6448
                     /locus_tag="PLM68_00355"
     CDS             6017..6448
                     /locus_tag="PLM68_00355"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOT53855.1"
                     /translation="MYKKTLALIAHDNKKKGLIEWVEFNRERLSRHKLVCTGTTGRMV
                     EEATGIHVSTLKSGPLGGDQQMGALIAEGKIDMLIFFWDPMQPQPHDVDIKALLRISS
                     LYNIPTACNRSTADFIISSTLFDSSYIPAPESFDDYLNRPL"
     gene            complement(7005..7976)
                     /locus_tag="PLM68_00360"
     CDS             complement(7005..7976)
                     /locus_tag="PLM68_00360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT53856.1"
                     /translation="MDLSVVISLYNERESLPELLERIRKVLGSPLGEDQHDPPEYEVI
                     FVDDGSTDGSWDFITEQAEADSRVHGICFRRNYGKSAALFCGFSAAQGNVVITMDADL
                     QDDPAEIPALYRMVVRENLDLVSGWKKKRKDGLFTKNIPSRIYNVTARMVTGIKLHDM
                     NCGLKAYRREVVKSIEVYGEMHRYIPYLAKEAGFTRIGEKPVVHARRKYGKSKFGLSR
                     FVNGYLDLLTLWFLARFGKKPMHIFGLFGSLMFLAGGVIALWLIIQKLYWQSHGLPYR
                     AVTEQPLFYLSLLAIVLGMQLFLAGFIGELVSRNAPERNHYHIKDKR"
     gene            complement(7993..8499)
                     /locus_tag="PLM68_00365"
     CDS             complement(7993..8499)
                     /locus_tag="PLM68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOT53857.1"
                     /translation="MNQSFWNKAAISGILLALVSVFFIVLQSVLTPGRGLSILFWILK
                     LAATLGLLYYFMKDFGKGKESYTYADSLKFGLAVSFCSSIIAAAYYYFHVMFLFPDTA
                     NQLSNALLTGFEQAGVTAEDFNTDKFIARLPVIITISQLIYYNILGLLWSSLLANGTK
                     HKIVSTPF"
     gene            8597..10531
                     /gene="thrS"
                     /locus_tag="PLM68_00370"
     CDS             8597..10531
                     /gene="thrS"
                     /locus_tag="PLM68_00370"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT53858.1"
                     /translation="MITITFPDGKTKQYDNGITGYQIARDISPGLAAAVLGMKVDGTL
                     TDIRIPLKKDASVRFLKWEDEEGKQVFWHSSSHLMAEAIEILYPGVKFGIGPAIENGF
                     YYDIDLEGRQLSEADLETIERKMLEMAKTGQMFERREVSKEEAMRTYLDKGDPYKCEL
                     INDLEDGTISFYSNGSFTDLCRGPHIPDTSVIKAVKLTSIAGAYWRGDENRQMLTRIY
                     GITFPKKSLLDEYLLMLEEAKKRDHRKLGKELELFTFSQRVGQGLPLWLPNGAALRER
                     LENFLKDVQKVHGYQQVITPHIGQKELYVTSGHYAKYGASSFRSIKTPQEGEEFMLKP
                     MNCPHHCEIYKAKPHSYKDMPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQIKEEFCKVIDIVLYIFKTLSFNEFTVQISLRDQKDTSKYIGSDENWEKAEKAIIE
                     AAAEKNLETQVVYGEAAFYGPKMDFMVRDAIGRKWQLGTIQVDYNLPERFELEYTGAD
                     DKKHRPVMIHRAPFGSMERFVAVLLEHTGGKFPLWLTPRQVVVLPLSEKYNDFAKKVC
                     DFLNNCELRASLDDRNETVGKRIRENELKRIPYLLVVGEKEESAGTVSVRRQGEGDKG
                     TMKTEEFAALLRKEIKESIN"
     gene            10645..11154
                     /gene="infC"
                     /locus_tag="PLM68_00375"
     CDS             10645..11154
                     /gene="infC"
                     /locus_tag="PLM68_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT53859.1"
                     /translation="MNAEIDAPQVRVVGDNIEKPGIYPLAQALKMAEKMNLDLVEISA
                     QTDPPVCKIIDYQKFLYQKRKKAKEMKANASRVIVKEIRFGPNTDEHDYQFKLKHAIE
                     FLKEGAKVKAFVFFRGRSIIFSEQGEKLLLRFAVDLEDYGKAEQMPKLEGKRMIMMLG
                     PNKANIKKK"
     gene            11171..11365
                     /gene="rpmI"
                     /locus_tag="PLM68_00380"
     CDS             11171..11365
                     /gene="rpmI"
                     /locus_tag="PLM68_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT53860.1"
                     /translation="MPKMKTNPGAKKRFTLTGTGKVKRKHAYHSHILTKKTNKQKRNL
                     THSAVVDKADEKRIKELICA"
     gene            11449..11793
                     /gene="rplT"
                     /locus_tag="PLM68_00385"
     CDS             11449..11793
                     /gene="rplT"
                     /locus_tag="PLM68_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT53861.1"
                     /translation="MPRSVNSAASKARRKKILKQTKGNFLARRNVWTVAKNTYEKGLT
                     YAYRDRKNKKRTFRALWIQRINAAARMHGMSYSQFMGKMNKANVGLNRKVLADLAMNN
                     PQAFEAVINSIK"
     gene            11802..13136
                     /gene="lepB"
                     /locus_tag="PLM68_00390"
     CDS             11802..13136
                     /gene="lepB"
                     /locus_tag="PLM68_00390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT53862.1"
                     /translation="MNRWIKFSLVAAGYLAWVIWLGNLWWLFGLLVIFDIYITKKVRW
                     AFWKGNAKKEGKRNVLLEWVDAIIFALIAATFVKSFFFEAYMIPTSSMENTLMTGDYL
                     FVGKLKYGPKRPQHPLTIPLTHNVIFGKESYSRLIERPYKRMAGFTQVSRDDIVVFNF
                     PQGDTVLVNYPTDDYYTHVRLNGRKNTLEHYGPVKVRPSGKEDNYVKRCVAVAGDTLH
                     VRDGKVHVNGVEQPAFSGIRNTYTVVTDGQPLNQRLLDKYGISPEETYFDNRIPGYPS
                     LPLHEEAAVEMAALRNIVSVTQNVDVFPPDYPDSQMMLFPFVTSSDFPWTRDNYGPLW
                     IPRSGATVTLTPQNIPLYKRIITVYEGHSFEEKDGEYYIDGQLTTDYTFGMDYYFMMG
                     DNRHNSLDSRYWGFVPEDRIVGNPLVIWFSTDKHKSFPSRIRWERILKFRFK"
     gene            13211..13453
                     /gene="rpmB"
                     /locus_tag="PLM68_00395"
     CDS             13211..13453
                     /gene="rpmB"
                     /locus_tag="PLM68_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT53863.1"
                     /translation="MARVCQVTGKKRMVGNKVSHSKRRTKREFAPNLKTKRFWSEEEG
                     RFITLKVSAAGIRTINKNGLAATLKASREKGLIDIV"
     gene            13456..13638
                     /gene="rpmG"
                     /locus_tag="PLM68_00400"
     CDS             13456..13638
                     /gene="rpmG"
                     /locus_tag="PLM68_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT53864.1"
                     /translation="MAKKENRVQIILECTEQKESGVPGISRYITTKNKKNTTQRLERR
                     KYNPYLKKVTLHKEIK"
     gene            13654..13818
                     /locus_tag="PLM68_00405"
     CDS             13654..13818
                     /locus_tag="PLM68_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOT53865.1"
                     /translation="MAKKAVATLKDKSAEKGSVKCIRMVRSERSGSYVFKESIVTPDK
                     EKDFFAGKQE"
     gene            13888..14811
                     /gene="ftsY"
                     /locus_tag="PLM68_00410"
     CDS             13888..14811
                     /gene="ftsY"
                     /locus_tag="PLM68_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT53866.1"
                     /translation="MQNGEILNKGLEKTKKGLFSRLTRVVMGRSRVDDQVLDHLEEAL
                     IASDVGVDTTLRIIDRIEERVRRDKFMNTEELNRYLREEIESLLLENKATNQVMPFDF
                     SKKPFVILVVGVNGVGKTTTIGKLASQLKNEGKKVMLGAADTFRAAAVEQLTIWSERA
                     GVPIVKQAMGSDPAAVAYDSLASAISRDMDVLLIDTAGRLHNKVNLMNELSKIRKVLQ
                     KLIPDAPHEVLLVLDGSTGQNAYQQASEFSKATDVTALAVTKLDGTAKGGVVIGISDM
                     LQVPVKFIGLGEGINDLQVFDKKAFVATLFE"
     gene            14821..17268
                     /gene="pheT"
                     /locus_tag="PLM68_00415"
     CDS             14821..17268
                     /gene="pheT"
                     /locus_tag="PLM68_00415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT53867.1"
                     /translation="MKILYNWLQDYFPLDMTAAQTAQVLTEIGLEVESLEITETIPGG
                     LRGVVTAEVVSCEKISGSDHLSLTTVFTGDGDPVQVVCGAPNVAAGQKVLLATVGTQL
                     AFSNGNQVTIKKSKIRGAESLGMICAEDELGIGTSHEGIMVLPPDTPVGVPAAEYLNL
                     PTYEVFEIGLTPNRIDAASHLGVARDLAAWYASRGKELAVKRPDVNAFRENGTVAGVQ
                     VEVHAPEAAPRYSGITLDNIRTGPSPLWMQDRLRMAGIRPISNVVDITNYVMLETGQP
                     LHAFDREHIQGDIVVVRMADAGSRFVTLDGQEHILDGEDLMICNQKEPMCIGGVFGGL
                     GSGVTDRTTSIFLESAWFHPAFIRRTSRRHGLKTDASFRFERGADPDATIYALKRAAL
                     LMQEICGARVAGRIIDRYPNPVLPARVELDYNTMFSLMGNNIGENTMETILRALEIKL
                     VARNEKTVTVEVPPYRVDVKRECDVVEEVLRIYGYNNIELPGRISSSMGQASKPEPHR
                     VRDDVADHISALGFREIMCNSLTGAAYYEHLETYPLRSLVRILNPLSNDLNAMRQTLL
                     WGGLETIAYNINRQQYDLRFFEQGNVYSLNGSGDTLQGYREQQNLALFITGNDRPQNW
                     HTAARGSDFYILKSYVDDIAGLFGLDLLRLESLQAPTDLFEEGIRYMVKDKVLADLGR
                     VGRSCLNILDIRQDVYAAEINWSLFFSMIRNNRTQFNDLPRFPEVHRDLALVVDESVS
                     YRQLQGIAFKTEKHLLKSVGLFDVYMGKGIPEGKKQYALSFVFQDPQKTLTDVYVEQI
                     INRLLEAFSKEAGAILR"
     gene            complement(17260..21723)
                     /locus_tag="PLM68_00420"
     CDS             complement(17260..21723)
                     /locus_tag="PLM68_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT53868.1"
                     /translation="MRVFRNILRTLLFILVISLTAAAIIVQLPSVQSRLAGRAAQWLS
                     EATGAEVMVGEVNFVLFNRLIIKDLLIRTAPEDTLLHSKKLSVRIGHVSALEKKIVLR
                     QVVLSEGSYHLIIQDSVTNIETLFPPSAGKDTTSRPFTWDIRSNRVILEDFAYNMINR
                     DTGPTHNNPECMDFSDLHISDISIDIRDARYCNGVVEARLDRLSCRERSGYLVHHLEG
                     DVTVDSKEALIKNLVIRDNHSYITAHHFLMHYGTSRNLSDYLNKVVMELDLDNAYFSF
                     RTIGYYAWSFPEDFTLAVRATGKVTGTVSSLGADNLDIRSETGRTRIGCGFLLRGLPE
                     SEETTLQVDIDELHSDMNDLNGILYSIARVDLKNLEEATGSRTPFHFRGRLAGLLTDF
                     AIDGDLDGHFGSLSLDMLVNNNIAPPGVLLSGVADLRDLDLGLFTGVENLGRCSLYTK
                     ASVTIREGGISSLHAVIDTLFLARLDFNQYSFQDIKMMGEFRNGIFNGKMIASDPNLQ
                     FLFQGRADLTRSAGTAADFFANIAYADLKLLNLYKNDSLAVLSGLLQADFSRINNLGD
                     IEGTIHASGLSLLNSRGTHALDSLILESTRTEDRSRYRLVINSDALQAEYTGEEGFMG
                     FVKRLVSRNLFRHVPSYFSSWTDYDPDSTVNGQAVFQMTVRNSVFFEEVFLPGLYIAP
                     GTNFSLHDYGNDSIRSVIRSERLGYQDHNLRNLDILAVARPSDIRADIQCRDLSLGPL
                     KADTLRIEAVAGNDRMNLDLFYNPGHDKKNHAIIGSVFNFSTEEGSDIPVIKAHLASD
                     SIMINRKKWTLHSDSLMLSGGKLALNNVLLQHIIGHEGVESFLINGALSQQPEDTLFV
                     KLSRFDASLFNTFLPASYPFTFRGSFTGQGYLTDFYGERRFMADIVGERFFVNDTLAG
                     DLRLKSSLDPQADYLQLEASTTLPGGQVPLLATGHYHPADKKLDLKATFNHFEAAIAG
                     PFLTGLVSDVHGKITGDMYLSGTLPYLDLVSDNTRVDKLNFTVDYTKVPYVLSGPVRL
                     TREGLYLPKDTLLDAGGNYAIVQGGLKYRQFRDIRADLNMSFTNFQGLNLDEKDNDTF
                     YGKAFATGNLIIRGPLNDILLDLSIRPEPNSVIHIPLSSASQARQTSLLTFFEAPVPY
                     TGRDPYTLAAMEQKKIKANDSRLTFNMKTELTPNAEILLEINKTTGDIITARGNGVIN
                     LGVDPRDDSFSITGDCTVESGSYLFGLQGIVSKRFQILPGGTIFFNGDIETTQLNLTA
                     SYKTKASLNTLLSDTTMISNRRDVDCQILMSGNLMNPTLGFNIDIQDIDPMTRARVES
                     ALSTDDKMMRQFVTLLISSSFIPEQQSGIVNNSNILYSNATEILSNQLNNIFVQLNIP
                     LDVGFNYQPNQRGDLFDVAISTRLFNNRVIINGNMGNNPYTRNENDLVGNVDIELKLD
                     KKGKFRLKAFSHAADQYSNYLDNTQRSGAGFVYQEEFNTFGQLWRRWLKLP"
     gene            21790..22841
                     /gene="tsaD"
                     /locus_tag="PLM68_00425"
                     /pseudo
     CDS             21790..22841
                     /gene="tsaD"
                     /locus_tag="PLM68_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            complement(22863..23393)
                     /locus_tag="PLM68_00430"
     CDS             complement(22863..23393)
                     /locus_tag="PLM68_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT53869.1"
                     /translation="MTISEEKVVSLSYTLVVDGDVIETVKDDKPMRFIYGLGYLLPAF
                     EEQIMGKKTGDEFSFTLSPKDGYGEVDPNAIVELSKDIFKVNGKVEEGLLVKDKVIPM
                     QDSRGNQLNGIVEEVLEDMVKMNFNHPLAGCELNFSGKVVEIREATEKELTEGLYGER
                     AASACSGSCQGCSGCN"
     gene            complement(23390..24814)
                     /locus_tag="PLM68_00435"
     CDS             complement(23390..24814)
                     /locus_tag="PLM68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOT53870.1"
                     /translation="MKKPSLLVSLIPVILLVAILLSSVLVFGDKVTAGPSQFALLLVA
                     VITFAIARLKYGISWSQAEESMLSHLKNTYPSILILISIGALTGSWMTSGIIPSMIYY
                     GLKVIHPAIFLPLSFVITALISLVTGSSWSTVGTFGVALLGAGQIIGLPTPWLAGAII
                     SGAYFGDKMSPLSDTTNLASSFCGVPLFTHIRYMLVNTGPVFVICLIIYVVAAIWIPV
                     DNAVDIQSELTALDNTFNISAWFLIIPVITIVLVVKKVSPFVTLFLSAIAGAIVAVLF
                     QPHICYQIAPGRFAAAIKILSTHVSVETGDQLLNNLTSTNGMAGMMNTVWLILCVIAY
                     SGVMTASGMINTITDNLLKLMRNTVSTIGTTLGTCLFFNMTLGDQYMAIILPANMFGD
                     TYRKRGLAPEVLSRTVEESGTVTSVLVPWNTCAVAQSTVLNVATMTYLPYCFFNIITP
                     IFSLFAAAIGYKIRKLHTNNNNLS"
     gene            complement(24824..26149)
                     /locus_tag="PLM68_00440"
     CDS             complement(24824..26149)
                     /locus_tag="PLM68_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT53871.1"
                     /translation="MKQKDLLKQMCSYALEMSLKKGATHARVAATSNNANGMSVLNDS
                     LETIEKAVDSSLTLHLFVEGRYGTCSTNKVDPSGLDKLLDDAIASVKLLAPDPFRKLV
                     PKELRYEGPPLYSAHSGSVDTGVKKAFLLDCACAIWNKEPALISVMADYSDHRQWLHL
                     ADSDGLHCTSEETWYNYSVECTVRGKGDARPSDWDFQGGIHPWDIGIGSGLPCTCAAT
                     ALERALAKRNPRKIVSGRYRVIVENRVAATLLAPLIDAMSGSSLQQQNSFLLDKLGEN
                     IASSVLTLIDDPRRKGTPGCRFFDSEGLATAKRAVIEAGRLNMYFLSTYYAEKMQMHP
                     TISSPSLLLLKEGKGNAATLAGLIKNGIFVTGFNGGNCNTTTGDFSYGIEGFYVEHGQ
                     ITYPINEMVMTGNMLDLWSRLLAAGDDALKTMSWQTPSLLFDDIEFSGL"
     gene            complement(26146..27546)
                     /locus_tag="PLM68_00445"
     CDS             complement(26146..27546)
                     /locus_tag="PLM68_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT53872.1"
                     /translation="MDLTTAIEMDSKKLLNLALQQGGDYADLYMQQTTFHSIVLRDGE
                     VNAASTHNEGGLGIRVVSGQNTGYAYTESTLPRDREEAARTASYIAQGLIRRTKPAPI
                     ATLNLPDRYRIVRPWEGFSPQEKMPFLYDLNERIFSADKRVEKVTAQLSDATSHIECY
                     NSLGIHCSDIRPMASLSVTCVMESGGQRESASASRSFRMGGEFLHSAMIRELAQEVLD
                     RTSFLFGAKQPNGGEMPVVMAAGGSGILLHEAIGHAFEADFARKKVSIFSDKMGQRIC
                     NPGITVIDDGTIPCNRGALNVDDEGVPGQKTYMVTDGILTSFLHDRISAGYFHTEPTG
                     NGRRESFRCMPLPRMRATYMDNGRDTEEALIASVKKGLYVDTFSNGQVQIGAGDFTFY
                     VKSGYLIENGKLTQPIKDTNIIGNGPEALARITGVANNCIIDNGTWTCGKGQTCAVSC
                     GMPSVLVASLTVGGRV"
     gene            complement(27524..29110)
                     /locus_tag="PLM68_00450"
     CDS             complement(27524..29110)
                     /locus_tag="PLM68_00450"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOT53873.1"
                     /translation="MRRFSGVLHCLAVLALSLTLFTGCREAVQEIELSVSAEKINLPQ
                     SGGWVDIIVYTNTSWRLENPQGYDIVPETGTGSASLRLSVEENPNLGTPLTGNFTIKA
                     GVLAVSVEITQAGIPPLLDIGETVAEYDYRAGSYTIHVTANLPWEAHSDRTWCLVQPV
                     SGNGSGSLEISFGENRDPQTRTAVITIGNETLNLFREFLLTQTGFVLDPRMQDSLALV
                     EFFMDTEGETWKKNKWDLSATMDSWEGVRLNAAGRVDSLSMASNVISRQSPIPASIGK
                     LTALEHLTLNSNNFTGTLPQALGELVLLKRLSISSNPGITGNIPAWIGNLTALERLSF
                     SSMTGLTGGLPEELFNLASLTDLNLVGCTGLGGPIPAGIGKLTGLTNLQMSNVPFTGT
                     LPPEIGQLKVLRSLGLFNTKLELPLPDALFDLENLTSVLFQQNANFNGPLPAGFGRMK
                     TKSDRLSIRLENCNFTGTIPAEWAGIPDVTFQLRVQENRLSGTIPIIVKQHTCWTPSV
                     WNPAVYICPQQPGYGFDNCD"
     gene            29161..>30876
                     /locus_tag="PLM68_00455"
     CDS             29161..>30876
                     /locus_tag="PLM68_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT53874.1"
                     /translation="MERPHHKYPVLLVLLLLMTALPGRNSYAQQQQYALSGIVRDQTT
                     GEPVPYATVFLEDLGLWFISAEDGSFRFTAIPAGTHTLHVEILGYAKKTFTQKIERDI
                     TGLVLQIAEESLRLDEVVVTAEEGGRLNSSSRIEKQALDHVQPVSLRDVMQLVPGNLT
                     ENPGLNRVNQLTIRDIASNSANAFGTAVIMDGVTLSNDANLQVRKTSTIGTAGESTAG
                     SGVDARQISTDNIQTVEIIRGIPSAVYGDLTSGAVVVKTRSGVSPWQIRLKADPRLKQ
                     VYAGKGFSLGPEKGVVNIDGDYALSYGDVRTPADVYRRVNLQIGYSQTVRKKLTYNAR
                     MRLIYSDADNRTDPDNFLDNISRDRDMGLGLDINGTYRLNKKWVTNLEFMAAGDVTSQ
                     YSLEKTYRSQGRIPQSLAMTSGENLGFFTPYRYYSNLEVFGLPINVQARVVANLYGKY
                     GDITNKVLAGVEWTSRGNRGAGKVFDPYLSPDVNSTSTYRERSFRDIPFLNRYSVFAE
                     DKLRVPLGLVRALEIQAGVRFNGIVPDTRFSMDRYYSVEPRLNARFQILRQQEGFREL
                     SIRAGW"
BASE COUNT         8273 a         7255 c         7868 g         7480 t
ORIGIN      
        1 ggtccatggt cacctttatt cctgcatggc tgaggtcaat tttatgaatg acaaggggtg
       61 attttccctt gttcgaaagt gaaaaattgg tcttaatctc cgaaccggcc ggcgtcaggg
      121 ggaattcaac ggcactttta tccatttgcg gtatgggggc catttcctta tctgcccggg
      181 tcatgccgga agtattcatt ttgatggatc ccttcaccgt aagaacatgc aagggatccc
      241 gccggtcatt gataaccacc tggcaggtat acagcacatt gccccagtct tcctgcttcc
      301 gggtgtctat ggaatacact atcataccgc tttccgccgg attgagcctg gaaggcatgg
      361 cacttacctg caggttcttg tccggacttt cgaacttcag aaaaacggga agttttccgg
      421 tatttatgac ctggaccgaa tctgttttga ccatcccctg gcggatctgt ccaagctcca
      481 gttccctgtt gcgcagccta agcggaccca tcacgacagg atgggtatct tccaaagtga
      541 attttttctg gtgcacaaca ccccttatcc gcaaaatgaa tggttcaggg aatcctgaaa
      601 tatatactga catcatttta tcaaaagggt agggtccctg gtcattggaa taggtcacgg
      661 taatggtacc tttcgctgcc ggctgcagag gctctttggt ccattcgggt accgtgcatc
      721 cgcatgaaga tatgacggtc tggatgacaa tggctttggc cgagttgttg gtaagcgtga
      781 atgtatgtgt ttttggcccg tcattgatcc cgaaatctcc aaaatcatga acagttttgt
      841 cgaatgtcac ggcgccttcc tgctgtgcaa aagcagaaac ctggatcaag agggaagaaa
      901 acaggaaaac cagcagtctc atcatataaa gattgtcaat ttacaaaggt agtaagaatt
      961 taattcatat atttgtggca attatttaca acaaacagtc aaacaaccat ggcacacaaa
     1021 attaccgaaa actgcatagc atgcggaact tgtatcgatg aatgtcccgt agaagccatt
     1081 tcccccggtg acatctatgt tataaatcca gacgaatgca ttgattgcgg ggcttgcgct
     1141 gcagtttgtc cgtcaggcgc tattgaaggc gaatagtata aggtccactg aagatcttga
     1201 aagagactgg ccaatggtat attggccagt tttttttaga ggttattggt ccaaacccac
     1261 acctgatatg acttcgtgat aattgacaat attaatgtac ctttgtaagc tattatttca
     1321 agatttcaga tatggcttct tttataaatg ttataggaaa gatctttggc tccaaagcgg
     1381 acagggacct gaaagaactg aacccttacg tggaaaggat caaaaaggaa tacgacaggc
     1441 tcgataagct ggatcacgat gatttgcgtg cagaaacgca gaaaatcagg cagatcatcc
     1501 tggatcacat agcagaagac gaaaagcaga aagtagcgct aagggaacag ctggaagata
     1561 tggaaatcga agtggaaaag aaggaagccc tggccaccga agtggacaaa ctcaccaaaa
     1621 agattgatga aaagatagag gaggtgctgg atagcgtgct tccacaggtc tttgccatca
     1681 tgaaatccac ggcacggagg tttttcgaga acaagacgct taccgtcacg gcttctgaat
     1741 ttgacaggga gctttccact tcaagggacc atgtggaaat caggggagac aaagccatat
     1801 ggcacaattc ctggatggcc gggggtaacc gcataacatg ggacatggtc cattacgacg
     1861 tgcagctcat tggcggcgtc gtgctgcaca agggcaagat tgccgagatg gcaacagggg
     1921 aaggaaaaac acttgtggcc accctgccgg tttttctgaa tgcattggcc ggcaagggag
     1981 tgcatgtggt tacggtcaac gattacctgt ccaggcgtga ctcggaatgg atgggacccc
     2041 tctatgaatt tcacggactc cgggtggatt gcctggacaa gcatcagccc aactcggatg
     2101 aacgccgcaa ggcatatcgc gccgacatca cctttgggac aaacaatgaa ttcggctttg
     2161 actacctgcg tgacaatatg gccgtcaact cggatgacct ggtccagaga aagcaccatt
     2221 ttgccattgt ggacgaggta gactcggttc tggttgacga tgcccgtacc cccctgatca
     2281 tttccgggcc cgtggccaag ggagatgacc agcaattcaa tgaattcagg ccgattgtgg
     2341 aaaacctcta ccaggcacaa agagtcctgg tccagcaatt ccttgccgat gccaggaaac
     2401 tgatacagga cggagacgag gaaaatggag gaaagctcct gctcaaggca ttcaagggcc
     2461 tcccgaaata caatccgctc atcaaatacc tgagcgagcc cggtatcaag cagttgctgc
     2521 agaagaccga aaacttctat atgcaggaca acaacaagca gatgtaccag ataacggatg
     2581 acctttactt tgtcattgaa gagcagcaaa agtctgtgaa cctaactgaa aaaggtcatg
     2641 acctgattgc aaaaaaggta agcgatgcca ctttctttat cctgcccgat atgggtacag
     2701 aaatttccga actggaaaag aaaaacatta cagcagaaga aaaagcagcc gccaaagatg
     2761 ccctgttgaa cgattatgcc atcaagtcgg aacgtgtaca tacagtcaat caattgctca
     2821 aggcatacgc catgttcgat aaggatgtag aatacatagt ggtggacaat aagatcaaga
     2881 tcgttgacga gcagacagga aggatactgg aaggaagacg ttattcagac ggactgcacc
     2941 aggctattga agccaaggaa cgggtaaagg ttgaggcggc cacccagacc tttgccacca
     3001 tcaccctgca aaattatttc cgcatgtacc acaagctggc cggtatgacc ggtaccgcag
     3061 aaacagaagc aggtgagttc tggtccatat acaagctgga tgtggtggtc atcccgacaa
     3121 accgcccggt gataagaaaa gatgaggaag acctgatata caagacaaaa cgtgagaaat
     3181 acaatgctgt catagacaag attgaccagt tgaccaaagc cggaaggccg gtactggtgg
     3241 gaacaacctc tgtcgaggtc tccgagctgc ttagccgtat gctgaagatg cgaggtgtca
     3301 agcacaacgt gctcaacgcc aaacaacatg ccagggaggc cgaaatcgtt gcagaggcgg
     3361 ggcgggcagg tgcggtgacc attgccacca acatggcagg aaggggtacg gacatcaagc
     3421 tcagggaagg tgtcagggaa gccggaggtc tggccatcgt tgggacagag cgtcacgaca
     3481 gccgccgtgt agacaggcag cttaggggac gtgccggccg ccagggagac cctgggtcat
     3541 cggtgttttt catttccctt gaagacgacc tgatgcgcct cttcgggagc ggaaggattg
     3601 cagccgtggt ggacaagatg ggcatgaagg aaggagaagt gctccagcac tccatgttgt
     3661 caagttccat tgaaagggcg cagaaaaaag tagaagagaa taactttggc atacgcaaga
     3721 ggctattgga gtatgacgat gtcatgaatt cccagcgtga ggtgatctat acacgccgcc
     3781 ggaacgcctt gtacggagaa cgtgtagatg ttgacgtggc caatatgatg tcggactatg
     3841 ccgaatcttt catgcaacgg atggacggtg tcacttatga cgacctccgt tttgaactga
     3901 tacgccagat atcgattgaa cccggatttg acggggaagt tttccggaag gcatctaccc
     3961 cggaaatggc aggattgctt gcagagcaga ttcagaccac ctacaaccgc agggcagaag
     4021 ccctgatagc gcaggcctgg cccatcatca agaatattta ccagacccag cgggccactt
     4081 atgaaaatgt ggttgttccc gtaagtgacg gcaagcgtat ttaccaggtc attgtgaaca
     4141 tacagaaggc gtacgaaagc aagggaaagg aattgaccag gtccatcaac aagacgatca
     4201 acctggttgt catagatgag ttctggaaag aacacctgcg cgatatggac gatttgaaac
     4261 aatccgtgca gagtgcaacg tatgagcaaa aagatcccct gctgatatac aaattcgaga
     4321 gtttcaacct ttttagtgaa atgcttgaaa aaatcgcccg cgaagtcttg tctttcctta
     4381 tgaaggccca tatccccctg cgtcaggacc aggacaggct ggataaagca ggaagcgaaa
     4441 gaaaacgcac cgatttgagc aacacccaga cctcacgtcc cgatttgctt acccagtctg
     4501 ttgaaccccg ttcaaacgca cctgtccacg tagagaaaca agtgggaaga aacgatccct
     4561 gtccctgcgg gagcggtaag aaattcaaac aatgtcacgg taaaaattaa tctatccacc
     4621 aagaatatat gctatacgat ctaataatct taggttccgg tcctgcggga tatacggctg
     4681 cggtccgggc ttcccagctg ggattcaaga ctgcagtctt agaaaggggc cccctgggag
     4741 gtgtctgtct taactggggt tgcattccga ccaaagccct gctcaaaagc attgaagtac
     4801 taaatttatc aaaaagtgcc caatcctatg gggtgaaact agccccggga gctgagcccg
     4861 atctggctgc cattgtttcc cgcagccggg aagtctcggc ccagatgtcc aaaggaattg
     4921 agttcctgtt caacaagtac aaagttgagg tgattcatgg caggggctcc gtactggggc
     4981 ctggaaaagt ttccgcgggg gataagattc tggaaacaaa aaggatactg atcgctacgg
     5041 gagcaaaacc caattccctg cacggagcct ccacagacgg gaaaaagatc atcgcttacc
     5101 gccaggccat ggttcccgaa accattcccg ctaccatagc agtgattggt tccggagcca
     5161 taggcaccga actggcttat ttctaccact cactcggtag taaagtgacg atcattgaat
     5221 atatggacca gctggttcct acggaagatg ccgacgtggc agccctgctg agccgctctt
     5281 tccgtaaagc aggaatccag gtgatgactt ctgcccgcgt gaaatctgcc gataaatctg
     5341 tcggaggatg ccgcctggaa gtggaaacaa agaaagggac agaattcata gatgccgatg
     5401 tggtgctgtc tgctacggga gtggtgccca atacatcaaa tttgggcctg aaggaagcgg
     5461 gcgtgaaaat ggaacgcgaa aggatcattg tagataagaa tttcatgacc tctgttcccg
     5521 gaatttatgc tgcaggcgat gtgctcccga caccggccct ggcccacctg gcatctgctg
     5581 aagccctgtg ctgcgtggag cgcatggcag gtctggatgt tccggatgtg gattacgcta
     5641 atatacccgg atgcatttat gcaacgcctg aaattgcgtc tgtgggttta cgtgaaaaag
     5701 atgcaaaagt aaaagggatt gaggtcagca ttggcaaatt cccctttacc gcatcgggga
     5761 aagctgcgac agccggtgaa cgtgaaggtt ttgtaaaact tatcttcaac gcacaggaca
     5821 gccgccttgc aggggcacac atcataggtg cacatgctac cgagatgata ggcggtcttg
     5881 tggcatgcat tggcatgaaa attaaagaca tggacctggc acgcactgtt ttcccgcatc
     5941 ccaccatgag tgaggggatt atggaggctg cagccttagc ctgcggtcat tgtgttaatt
     6001 tgtaatcaat agctttatgt acaaaaaaac cctggcttta atagcccatg acaataagaa
     6061 gaaaggtctg atagagtggg tggagttcaa ccgggaacgc ctctccaggc acaaactggt
     6121 atgtacaggc acaaccggcc gcatggtgga agaagccacc gggatacatg tttccacgtt
     6181 gaaatcaggc cctcttggag gggatcagca gatgggtgcg ctgatagctg aaggtaagat
     6241 agacatgctt atctttttct gggatcccat gcaaccgcag ccccacgatg tggatattaa
     6301 ggcattgctc cggatttcaa gcctttacaa tattccgact gcctgcaata ggtcaaccgc
     6361 cgatttcatc atttcttcca cgctcttcga cagttcctat atcccggctc ctgaatcatt
     6421 tgatgattac ctgaaccgcc ccttgtaacc ggcagtcact cccccataaa gaaccgggag
     6481 cttttgtttt agtcgaggct cccggctctt tatgggggag ctttccagtc atttattagt
     6541 attcttgcac aaatggctgc acggtggtct tcaaagcagt gcgccaaagt ccttttgcat
     6601 ttataatagg ctgcttcgcg acaccactct gtttatcggc ggtttttgcg ccaacaggta
     6661 agtgaaaaca cctgtatcta ctagcgtcaa ccccccttcc ggaagtaact ctgctccatc
     6721 cacaaaaaga agggggagaa gttccgcgta cacttgatcc attatgttag atatcagcac
     6781 tataacaaaa gagctttccg atagggctct tttgttgatc acttgttaaa caggataata
     6841 catcatgtgt acgcggtgct cccagaggac tgtcgctgcc accactggga acggagagcc
     6901 tcggctaaac tctccgttcc cagtggtggc agcaacaaca ggaggcagca aacgcacaga
     6961 aagctcccgg taaaaagaaa aaagtgtgta agacaccctg ggcgttagcg tttgtctttt
     7021 atgtggtaat gatttctttc cggggcattg cgcgaaacca gttccccgat gaagcccgcc
     7081 aggaacagtt gcattcccag tacaatggcc agaagagaca ggtaaaagag cggctgttca
     7141 gtgacagcgc ggtaaggtaa tccatgggat tgccagtaaa gtttctggat gatcagccat
     7201 aaggcaatga caccgccagc caggaacatg agcgatccga aaaggccgaa aatatgcatc
     7261 ggcttttttc caaaacgggc caggaaccac aaagtcagca agtcaaggta accattgaca
     7321 aaacggctca atccgaattt gctctttccg tatttgcgcc gggcatgcac aacaggtttc
     7381 tcaccaatcc ttgtaaaacc ggcttccttt gcaagataag ggatgtagcg gtgcatttct
     7441 ccgtaaacct caatgctttt gacgacttcc ctgcggtaag ccttcagccc gcagttcatg
     7501 tcgtgaagct ttatccctgt caccatcctg gcagtgacat tgtatatgcg cgaaggaata
     7561 ttcttggtga acaaaccgtc cttgcgtttc tttttccagc ccgagaccag gtcaagattc
     7621 tcccggacca ccatccggta aagggcggga atctcggccg gatcgtcctg cagatcggca
     7681 tccatggtta tcaccacgtt cccctgagcg gcactgaatc cgcaaaaaag tgctgccgat
     7741 ttaccgtaat ttcggcgaaa gcagatgcca tgaacacggg agtcggcctc ggcctgttcc
     7801 gttatgaaat cccaggagcc atcggtgctg ccgtcatcca caaagatcac ctcatattct
     7861 ggaggatcat gctggtcttc tcccagcggg gaaccaagga cctttctgat acgttccagc
     7921 agttcgggaa gcgattcccg ttcattgtac aaagaaatta ctaccgaaag gtccatgcgg
     7981 gaagtttact ggttaaaagg gtgtggacac aattttgtgc tttgttccat ttgccagcag
     8041 tgaagaccag agcagaccca gaatgttgta atagatcagc tgggatatgg ttatgataac
     8101 gggcaaacgg gctatgaatt tgtctgtgtt gaaatcttct gcagtaacac cggcctgttc
     8161 gaaacccgtg agcaatgcgt ttgagagctg gtttgcagta tcggggaaca ggaacatcac
     8221 atggaaatag taataggctg cagcaattat ggaagaacag aacgacacgg caagaccgaa
     8281 tttcagggag tctgcatatg tgtagctttc ttttcccttc ccgaaatctt tcatgaagta
     8341 gtaaagcagc ccaagagtgg cagcaagttt caggatccag aataaaatac tgagcccccg
     8401 cccgggtgtc agcaccgatt gcaatacgat gaaaaaaacg gagaccaggg ctaacagtat
     8461 gcccgatatg gcggctttgt tccaaaatga ctgattcatt cgcacatggt tagatttgat
     8521 gtacaaagta actcaatttt ttaaaaaata cctaacttta caagttggta caaaacagaa
     8581 cattttaatt cgacttatga taacgattac ttttcccgac gggaaaacaa aacaatatga
     8641 caacggaata accggttatc agattgccag ggatatcagt ccaggactcg cagccgccgt
     8701 tctgggcatg aaagtggacg gtacattgac agatatacgc attcctctga aaaaggatgc
     8761 ctcggtacga tttctcaaat gggaagacga agagggaaaa caggttttct ggcactcctc
     8821 gtcccatttg atggcagaag ctatcgagat cctttacccc ggcgtcaaat tcgggatcgg
     8881 accggccata gagaacggct tttactatga tattgacctg gaaggacgtc agctttcgga
     8941 agccgacctg gagaccatag aacgcaagat gcttgaaatg gcaaaaacag gccagatgtt
     9001 tgaacgcagg gaggtaagca aggaagaggc catgaggaca tacctggata aaggagatcc
     9061 gtacaaatgc gaattgatca atgacctgga agacgggact atctcttttt attcaaatgg
     9121 ttctttcaca gacttgtgca ggggtcctca tattcctgac acgtctgtga tcaaagctgt
     9181 caaactcact tccattgccg gtgcctactg gcgcggggat gagaatcgtc agatgctgac
     9241 caggatctat ggtataacat tcccaaaaaa atccctgttg gacgaatacc tgctaatgct
     9301 tgaagaagct aaaaaaaggg accaccgcaa gctgggaaaa gagctggagc ttttcacttt
     9361 ttcacaaagg gtgggacagg gacttccttt atggttgccc aatggagcag cgctgaggga
     9421 gcgcctggag aatttcctga aagatgtgca gaaagtccat ggttatcaac aggtcatcac
     9481 cccccacata ggtcagaaag aactgtatgt gaccagcggt cattatgcca agtacggcgc
     9541 cagcagcttc agatccataa agacacccca ggagggtgag gagttcatgc tcaaacccat
     9601 gaactgccca catcactgcg agatttacaa ggcaaaaccg cactcctata aagacatgcc
     9661 ggtacggctg gctgagttcg gcaccgttta ccgttatgaa caaagcggcg agttgcacgg
     9721 attgacaagg gtccgcggct ttacacagga tgatgcgcac atattttgca gacccgacca
     9781 gatcaaggaa gaattctgca aagtgataga tatcgtgttg tatatattca agacgttaag
     9841 tttcaacgaa tttacggtac agatatccct gcgcgaccag aaggacacct ccaaatacat
     9901 cggttcggat gaaaactggg aaaaggcgga aaaagccatt attgaagctg cagcagaaaa
     9961 aaatctggaa acacaggtgg tatatggaga ggctgccttt tacggaccca agatggattt
    10021 catggtacga gatgccattg gaagaaaatg gcaattgggt actatacagg tagattacaa
    10081 cctgccggaa cgttttgaac tggaatatac tggagcagat gataagaaac acaggccggt
    10141 gatgatacac agggcgccgt ttgggtcaat ggagcgtttt gttgctgttt tactggaaca
    10201 tacaggtggt aaattccctc tttggctgac tccccggcag gttgtcgtat tgcctttgag
    10261 tgaaaaatac aatgattttg caaaaaaagt ttgtgatttc ttgaataatt gcgaacttcg
    10321 cgcctcgctt gacgaccgta acgagactgt aggtaagcgt ataagggaga atgagttgaa
    10381 gcgtattccc tatctgctgg tggttggtga aaaggaagag tctgccggaa cggtgtcggt
    10441 tcgtcgtcag ggagaaggag ataaaggcac catgaaaacg gaggaattcg ctgcattact
    10501 ccgtaaagaa ataaaagaat cgattaacta aaaaaggagg attacattat cggaacgtcg
    10561 tacagacctc agtctacccc acagaaaagg aattatcagg gaaagaatta tcaaaggaat
    10621 tcccctcagg atgacggttc caggatcaat gccgaaattg atgctccaca ggtgcgcgtg
    10681 gtgggtgaca acattgaaaa gcctggaata tatccccttg cacaggcttt gaagatggca
    10741 gaaaaaatga accttgatct ggtggagatc tcggcccaga cagacccccc ggtctgtaag
    10801 ataattgact accagaagtt tttgtaccag aagcggaaga aagccaagga gatgaaggca
    10861 aacgcttcca gggtcatagt caaggaaata cggttcggtc caaatacaga tgaacatgat
    10921 taccagttta agctcaagca tgccatcgag ttcctgaaag agggtgccaa ggtgaaggct
    10981 tttgtgtttt tcaggggacg ttccatcatt ttctccgaac agggagaaaa actgttactc
    11041 cggtttgccg tggatcttga agattatggt aaagccgaac agatgcccaa actggaagga
    11101 aagcgcatga ttatgatgct gggcccgaat aaagcaaaca ttaagaaaaa ataaccatta
    11161 acaatagatt atgcccaaaa tgaaaaccaa tcccggagcc aagaagcgtt ttacgctcac
    11221 cggaaccgga aaggttaagc ggaaacacgc ttaccacagt catatcctga ccaaaaaaac
    11281 aaataagcag aaacgtaatc tgacgcactc cgctgtggtg gacaaagcag atgaaaaacg
    11341 catcaaagaa ctgatttgcg cttagttctg caattatatt aaccagattg aacagccggg
    11401 aagattctca aaggaggaga acgctgccaa tcgcaaagaa tttaaattat gccaagatcg
    11461 gtaaattcag cggcctcgaa ggccagacgc aaaaaaattc taaaacagac gaagggtaat
    11521 tttttggctc gccgcaatgt ctggacggta gccaaaaaca cgtatgaaaa aggtttgacg
    11581 tatgcatacc gcgaccgtaa aaacaaaaag cgcactttcc gcgccttgtg gatccagcgc
    11641 atcaacgcag ccgcccgtat gcatggtatg tcctactcac aatttatggg taagatgaac
    11701 aaggccaacg taggccttaa ccgtaaggtt cttgcagacc tggccatgaa caatccccag
    11761 gcatttgaag ctgtaatcaa ttccattaaa taatcagggt tttgaaccgc tggatcaaat
    11821 tctcactggt tgctgcaggc tacctggcat gggttatctg gctgggtaac ttatggtggc
    11881 tctttgggct cctggtcatt tttgatattt atatcacaaa aaaggtcaga tgggctttct
    11941 ggaaagggaa tgccaagaaa gaaggaaagc gcaacgtgct tttggaatgg gttgatgcca
    12001 ttatttttgc actgattgcc gccacctttg taaaatcctt cttcttcgaa gcctatatga
    12061 tccccacctc atcaatggag aataccctga tgaccgggga ctacctgttt gtgggaaaat
    12121 tgaagtacgg gccaaaaagg ccacagcacc cccttaccat tcccctgaca cacaatgtga
    12181 tcttcggtaa ggaatcatat tccaggctta tagaaaggcc gtacaagcgt atggcgggat
    12241 tcacacaggt cagtagggat gatatcgtgg tgttcaattt tccacaaggt gatacggtgc
    12301 tggtcaatta cccgaccgac gattactaca cgcatgtgag gcttaatggc cgtaaaaaca
    12361 cactggaaca ttacggcccg gttaaagtaa gaccctcggg gaaggaagat aattatgtaa
    12421 agcgctgtgt ggcggtagcc ggagataccc tgcatgtaag ggatggaaag gttcatgtga
    12481 acggagtgga gcaaccggca ttttccggga tccggaatac ttataccgtt gtgacagacg
    12541 gacaacccct gaaccagcga cttttggata aatacggcat atccccggaa gaaacatatt
    12601 ttgacaaccg catacccgga tacccgtccc tgcccctgca tgaggaagcc gctgtagaga
    12661 tggctgcctt gcgcaacatt gtatccgtta ctcagaatgt ggatgttttt cctcctgatt
    12721 atcccgattc ccagatgatg cttttcccgt ttgtcacatc ttccgatttt ccctggacaa
    12781 gggataatta cgggccttta tggattccca ggtcaggagc aaccgttacc ctgacaccac
    12841 aaaacatccc tttgtacaaa cgtatcatta ccgtgtatga ggggcattca tttgaggaaa
    12901 aagatggtga gtattacatt gacggtcagc tgacaacgga ctataccttt gggatggatt
    12961 actacttcat gatgggagat aaccgtcata attcactgga ttcacgatac tggggatttg
    13021 tccctgaaga caggattgtg gggaatccgc ttgttatatg gttttccacc gataagcaca
    13081 aatcattccc atcccggatc cgctgggagc gtatcctgaa gttccggttt aaataaattt
    13141 tgggtttcca aaaaaaatgc agatatttgc agccccttaa aaatgaataa atcacataaa
    13201 tttagatacc atggcacgag tttgtcaagt tacaggtaag aagaggatgg ttgggaacaa
    13261 agtgtctcac tctaagcgtc gtaccaaacg cgagtttgcc cccaatttaa aaaccaagcg
    13321 tttctggtcc gaggaggaag gccgttttat cacgttaaag gtttctgctg ccggaatccg
    13381 gactatcaac aagaatggcc tggctgccac attgaaagca tcaagggaaa aaggtttgat
    13441 tgatattgta taattatggc aaagaaagag aacagggtac agatcatact ggaatgtacc
    13501 gaacagaaag agagtggcgt tccggggata tcccgttaca ttaccacaaa gaacaaaaaa
    13561 aacacgactc aacgtctgga gcgcaggaaa tacaatcctt accttaagaa agtaaccctt
    13621 cacaaagaaa tcaaatagta aacacagatc attatggcaa aaaaagcggt tgcaactctt
    13681 aaagataaat cagcggaaaa aggcagtgtg aaatgcattc gcatggtccg ctcggaacgt
    13741 tccggatcct atgttttcaa agaaagcatt gttactccgg ataaagaaaa agattttttt
    13801 gcaggcaaac aggaataatt gccttacgga aatataaatg aagaccggtt ctgtaaggcc
    13861 ggtctttttt tttatatttg cattcttatg caaaacggtg aaatcctcaa taagggactg
    13921 gaaaaaacaa agaaagggct gttcagccgt ctgaccaggg tggttatggg acggtcaagg
    13981 gtagatgatc aggtcctgga ccacctggaa gaagccctca ttgcttccga tgtaggtgtg
    14041 gacacaacac tcaggattat tgacaggata gaggagcgtg ttcgccggga caagttcatg
    14101 aacacagagg agctgaacag gtacctgagg gaagaaattg agtcgctcct tttggaaaac
    14161 aaggcaacga accaggttat gcccttcgat ttttcaaaaa aaccttttgt gatccttgtt
    14221 gtcggggtga acggtgtggg aaaaaccaca accataggta aacttgcttc gcaattgaaa
    14281 aatgaaggaa agaaggtcat gctgggagca gccgatacat tccgggcagc agccgttgaa
    14341 cagcttacca tctggagtga gcgtgccggg gtgcccattg tgaagcaggc tatgggcagt
    14401 gaccccgccg ctgttgccta tgattccctg gcctcggcta tctcaaggga tatggatgtg
    14461 ctcctgattg acacagcggg ccgtcttcat aacaaggtga atttgatgaa cgaattgtcc
    14521 aaaatacgaa aagttttgca aaagctcatt cccgatgcac cccatgaagt gctgctggta
    14581 ctggatggct ccacgggaca gaatgcctac cagcaggcca gtgagttttc caaagccacc
    14641 gatgtgacgg ccctggcggt aaccaagctt gacgggacgg ccaagggagg tgtggtgatc
    14701 ggcatcagtg acatgttgca ggttccggtg aagttcattg gactgggtga gggaataaac
    14761 gatttgcagg tttttgacaa aaaggcattt gtggccacat tatttgaatg atcataaggt
    14821 atgaagatac tctacaattg gttgcaggat tactttcccc tggacatgac cgccgcacag
    14881 acggcacagg tgctcactga aatcgggctg gaagtcgaat cacttgagat tacagaaacc
    14941 attccgggcg gactccgggg tgtggtgact gcagaggtgg tctcgtgtga aaagatatca
    15001 ggctcggacc acttgtccct gacaaccgta ttcaccggag acggtgatcc tgtacaggtt
    15061 gtctgcgggg ctcccaatgt ggctgccggc cagaaagtac tgttggcaac cgtaggaacg
    15121 caactcgcct tttccaacgg gaatcaggta accatcaaga aatcaaaaat ccggggagcc
    15181 gaatccctcg ggatgatctg tgccgaggat gaacttggga taggtacatc ccacgaggga
    15241 atcatggtcc ttcccccgga cacgcctgtg ggtgttcctg cagctgaata cttgaatttg
    15301 cccacgtatg aagtatttga aatagggttg actcccaacc gcatagacgc agcatcccac
    15361 ctgggtgttg cccgcgatct ggcagcctgg tatgcctccc ggggaaaaga gttagccgtg
    15421 aaaaggcccg atgtgaatgc attcagggaa aatggcacgg tagcaggtgt acaggtggaa
    15481 gtccatgccc ctgaagcggc tcctcggtac agcggtatta cccttgacaa catcaggacg
    15541 ggtccttccc cgttatggat gcaggaccgc ctgcggatgg ccggcattag acccatctct
    15601 aatgtggtgg atattacaaa ttatgtaatg cttgaaacag gccaacccct tcatgccttt
    15661 gaccgggagc acattcaggg agatatcgta gtcgtacgta tggccgatgc cggcagccgt
    15721 tttgtaaccc tggacggtca ggaacacatc ctggacggag aggatctgat gatctgcaac
    15781 caaaaagagc cgatgtgcat tggcggggta tttggaggac ttggcagcgg tgtgaccgat
    15841 agaacaacct cgattttcct ggaatcggcg tggtttcatc cggcattcat aagaaggaca
    15901 tcaagacgcc acgggttgaa gacagatgcc agtttccggt ttgaaagagg agcagacccc
    15961 gacgcgacaa tatatgccct aaagcgtgcc gcactcttga tgcaggagat ttgcggggca
    16021 cgtgtggctg gccggatcat tgatcgttat cctaacccgg tgttgcccgc acgggtggaa
    16081 ctggattaca acaccatgtt ctcactgatg ggaaataaca ttggggaaaa caccatggaa
    16141 acgattctca gggccctgga aataaagctg gttgccagaa atgaaaaaac ggtcacggta
    16201 gaagtgcctc cctacagggt agatgtgaaa agggaatgcg atgtggtgga agaagtgctc
    16261 aggatatatg ggtacaacaa catagaatta ccgggacgta taagttcttc catgggacag
    16321 gcgtccaaac ctgaaccgca cagggtccgg gatgatgtgg ccgaccacat ttcagcattg
    16381 ggattcaggg agataatgtg taattccctg accggtgcag cctactacga acacctggaa
    16441 acgtatcccc tgcgctcgct tgtcaggatc ctgaatcctc tgagcaacga tctgaacgcc
    16501 atgcgccaga cactcctctg gggagggctg gaaaccattg cctataatat taacaggcaa
    16561 caatacgacc tgcgcttctt tgagcagggc aatgtctatt cgctcaatgg gagcggtgat
    16621 accctacagg gttaccggga gcagcaaaat cttgctctgt tcattactgg caatgaccgg
    16681 ccacaaaact ggcatacggc tgcccgtgga tcagactttt atatactcaa atcatacgtt
    16741 gacgatattg ccgggttatt cggacttgac ctgttacggc tggagagttt gcaggctcca
    16801 actgaccttt ttgaagaagg gatccgctat atggttaagg ataaggtatt ggccgacctg
    16861 gggcgtgtag gccgttcatg cctcaatata ctggacatac gtcaggatgt ttatgctgcg
    16921 gaaatcaact ggagcctgtt cttttctatg atccgcaaca acaggacaca attcaacgac
    16981 ttgccaagat tccccgaagt gcacagggac ctggcccttg tggtggatga aagcgtttct
    17041 taccgccaat tgcagggaat tgccttcaaa acggaaaaac acttgttgaa atcggtagga
    17101 ttgttcgacg tgtatatggg aaaaggtatt cctgaaggga aaaaacagta tgccctgagt
    17161 tttgttttcc aggatccgca aaaaaccctt accgatgttt atgtggaaca aatcataaac
    17221 cgcctgctgg aagcattcag taaggaagcc ggggccattt tacggtagct tcagccaccg
    17281 cctccacagc tgaccgaatg tattgaattc ctcctggtat acaaagccgg caccgcttcg
    17341 ctgtgtatta tcaaggtagt ttgaatactg gtctgcagca tgactaaaag ccttgagacg
    17401 gaatttaccc tttttatcga gtttcagttc gatatccaca ttacccacca ggtcgttttc
    17461 gttccgtgta tagggattgt tccccatgtt gccgtttatg atcacccggt tattgaacag
    17521 gcgggtggaa attgccacat caaagagatc acctctttga ttcggctggt aattgaaacc
    17581 gacatccaag ggtatgttta gttgtacgaa tatattattc agctggtttg aaaggatctc
    17641 ggtagcattg gaatagagga tattggagtt gtttacgatc ccggattgct gttcaggtat
    17701 gaaactgctg gaaattaaca gagtgacaaa ttgtcgcatc attttgtcat ctgtggacag
    17761 ggcactttca acacgggccc tagtcatggg atcaatatcc tggatatcaa tattgaatcc
    17821 cagtgtagga ttcatcaaat ttcccgacat gagtatctgg caatccacat cccgccggtt
    17881 actgatcatg gtggtgtccg acagcagggt gttaagggag gccttggttt tgtaggatgc
    17941 ggtcaggttc agctgtgtgg tttcaatgtc tccgttgaaa aagatagtgc ccccgggtag
    18001 tatctgaaac cgtttagata cgataccctg gagtccgaaa agataactgc cgctttcaac
    18061 agtgcagtca cctgtaatgg agaaggagtc gtctcttgga tcaactccaa gattgatcac
    18121 gccgttaccc cttgctgtga tgatatctcc agtggtcttg ttaatttcca gaaggatctc
    18181 ggcattgggc gtcagttcag tcttcatatt gaaagtgagc ctggaatcat tcgccttgat
    18241 tttcttttgt tccatggcgg ccagcgtata cggatccctt ccggtataag gaaccggggc
    18301 ctcgaaaaat gtcagcaggc tggtttgcct ggcctgtgaa gcggatgaca aaggaatgtg
    18361 gatgacggaa ttgggttcag gccggatgga aagatccaac aggatatcat taagggggcc
    18421 cctgatgatc aggttgcctg tggcaaaagc cttaccgtaa aaagtgtcat tgtctttttc
    18481 atccaggttc aatccctgga agttggtgaa actcatgttc aggtcggccc tgatatccct
    18541 gaattgcctg tatttcaaac ctccctgcac tatggcataa ttaccccccg catccaggag
    18601 agtgtccttt gggaggtaga gtccttccct tgtcaagcgg acaggccctg acaagacata
    18661 gggtactttt gtgtaatcga cggtaaagtt aagcttgtca acccttgtat tatcactaac
    18721 caggtccagg tagggtaatg ttccggacag atacatatct cccgtaattt taccgtgtac
    18781 gtcagagacc agtcctgtta ggaaaggtcc tgcaatagcg gcttcaaaat gattgaaagt
    18841 tgcttttaga tccagttttt tatcagcagg gtggtaatga ccggtagcca gtaaaggaac
    18901 ctgcccccct ggcagggtag tacttgcttc cagttgcagg tagtcggcct gtggatccag
    18961 cgaactcttc agacgcaggt cccctgccag ggtgtcgtta acaaaaaacc tttctccgac
    19021 aatatctgcc atgaaacgcc gttcaccgta aaaatcggtc agatatccct gcccggtgaa
    19081 ggaccccctg aaggtaaacg gatatgaagc agggaggaag gtattgaaca ggctggcatc
    19141 gaaccgggat aatttcacaa atagcgtatc ctcaggctgc tgtgacaagg ctccgttgat
    19201 aaggaacgat tccacccctt catgccctat gatgtgttgc agcagcacgt tgttcaaggc
    19261 aagttttcca cccgataaca tcaggctgtc cgagtgaagt gtccattttt ttctgttgat
    19321 catgatactg tcagaggcca aatgcgcctt tatcaccgga atgtctgaac cttcctctgt
    19381 agaaaaattg aacacagatc caataatagc atgatttttt ttatcatgtc ccggattata
    19441 gaacaagtca agattcatcc tgtcattgcc tgccaccgct tcaatccgta atgtatccgc
    19501 tttaagcggt cctaacgaaa gatcccggca ttgaatatct gccctgatat cagagggccg
    19561 ggcaacagcc agaatatcaa gatttctcaa attgtgatcc tggtagccca gcctttctga
    19621 acggattacg gaacggatgc tgtcattgcc gtaatcatgg agcgaaaaat ttgttccggg
    19681 tgcaatatac agaccgggca ggaacacttc ttcaaaaaag acactgtttc tgactgtcat
    19741 ttgaaagaca gcttgtccgt tgaccgtact gtcggggtca taatcggtcc aggaactgaa
    19801 ataggagggt acgtgacgga ataggtttcg cgaaaccagg cgtttcacaa agcccatgaa
    19861 tccttcttcg ccggtatact ctgcctgcaa tgcatctgag ttgatgacca gcctgtagcg
    19921 ggaacggtcc tctgttctgg tactttccag gattaaggaa tccagggcat gagtgccacg
    19981 tgaattgagc agagacagac cagaggcgtg aatagtccct tctatgtctc ccaggttgtt
    20041 tatccgggaa aaatctgcct gtaacaaacc gctaagtacg gcaagggagt cattcttata
    20101 aagattcaaa agtttcaggt ctgcataggc gatattggca aaaaaatcgg ctgctgttcc
    20161 ggctgagcgg gtcaggtccg cccgtccctg gaagaggaat tgcaaattgg ggtcggaagc
    20221 gatcattttt ccgttaaata ttccattgcg aaattctccc atcattttga tgtcctggaa
    20281 tgaatattgg ttgaaatcaa gacgggcaag gaaaagagtg tctatgacgg catgcaggct
    20341 gctgataccg ccctctctta tagtcacact tgccttggta tacaaagaac accgtcccag
    20401 attttctacc ccggtaaata gtcccaggtc cagatccctg agatctgcaa cacccgatag
    20461 caacaccccc ggaggtgcta tattgttgtt gacaagcata tccagggata aagaaccgaa
    20521 gtgaccatcc agatccccgt caatggcaaa gtcagtcagc aatccggcca ggcgtccccg
    20581 gaaatggaaa ggcgtccggc ttccggttgc ttcctcaaga tttttcaggt caacacgtgc
    20641 aatcgaatac aggataccgt tcagatcgtt catatctgaa tggagttcat caatatccac
    20701 ctgcaaggtc gtttcttccg attcaggcag gcccctgagc aggaaaccac aaccaatcct
    20761 tgtccttcct gtttcggaac ggatatccag attgtctgcc ccaagactgc tcacggtgcc
    20821 tgtcactttt cccgtggcac gcactgccag ggtaaagtct tcagggaaag accaggcata
    20881 ataacctatt gtcctgaaag aaaaataggc attgtccagg tcgagttcca ttacaacctt
    20941 gttcaggtaa tcggaaagat tccgggatgt gccgtaatgc atcaggaaat gatgcgctgt
    21001 aatgtaggaa tggttatccc tgatgaccag gttttttatc agtgcctctt tgctgtccac
    21061 tgtcacatcc ccttccaggt ggtgtaccag gtatcccgat ctttcccggc aggaaagacg
    21121 gtccaggcgt gcttcaacta ccccgttgca ataacgtgca tcacgaatat caatggatat
    21181 gtcgctgata tgcaggtcgc tgaaatccat acattccggg ttgttgtgtg taggaccggt
    21241 gtccctgttg atcatattat aagcgaaatc ttccaggatc acccggttgg aacggatatc
    21301 ccatgtgaaa ggccgtgacg tggtgtcttt accggccgaa ggaggaaaca aggtttcaat
    21361 attggtaaca ctatcctgta tgatcaggtg atatgatcct tcagaaagga caacctgacg
    21421 caaaacaatt ttcttttcca gcgcacttac atggccaatt cttaccgata gttttttgga
    21481 gtgcaacaaa gtgtcttcgg gtgctgtacg tatgagcaga tctttaatta tcagcctgtt
    21541 aaataacacg aaattcactt cgccaaccat tacttcggca cccgtggctt ctgaaagcca
    21601 ctgagcggcc cttccggcca accgtgactg aacggaaggc aactgtacta taatggcagc
    21661 cgctgtcagc gaaatgacca aaatgaacag gagggtgcgc agtatattcc gaaagacccg
    21721 catagttaaa gccttaactt gcaaacttac aaaaaaaatg gccacatttg cagactcaag
    21781 catggtacta tggcttatat tttaggtata gaatcttcct gcgatgacac atcggctgcc
    21841 gtactccggg acacgttgct cctctccaat gtaatggcct cacaggaggt acatggaaaa
    21901 tacgggggcg tggttcctga actggccagc agggcgcatc agcagcatat agttccggtc
    21961 gtaagccagg cgcttgagat tgcaggaata aggccttcgg acattgatgc catagctttt
    22021 acccaggggc ccggtctgct ggggtcgctc ctggtgggga cttcttttgc caagggtttg
    22081 tcattggctg caggggcacc attgatcagg gtgaatcacc tggtcggaca cattctgagc
    22141 cacttcatag atgtgagatc atcttcgggg aaggagcaca acccgcgtcg ggaaagaaac
    22201 ctgattttcc ttttttgtgt ctgcttgtct cggggggaca tacacaaatc attaaggtag
    22261 atgcccctct gtctttcagt atcctgggac aaacgataga tgatgctgtc ggagaggctt
    22321 ttgacaaagg ggcgaaactc ctggggctag aatatccggg tggcccccat attgacaggc
    22381 tgtcgcaaca gggagacccc gggatgtata gttttgcccg tccccatatc aaggatctgg
    22441 atttcagttt cagcggggtc aagacatcgt tcctttattt cttacgggac aagcagtcag
    22501 aggaccctgg ttttattgat aaaaacaagg cccaccttgc tgcaggcctt cagcataccc
    22561 tgatagacat acttgtggat aaagtggtga aagctgtcag gcagaccggc atcaatcggg
    22621 tagccctggc cggaggtgtt tcggccaaca gcggtctcag ggaacgaatg atgagagaat
    22681 caaaaaaaag acattgggat gtttttcttc ctcctttgtc ttttaccaca gacaatgcag
    22741 caatgatagc gattgtaggc tattacaaat acctggatgg caggtatgcc gggctggata
    22801 ttgccccggt cacaagaagc agccttatga ccggggaatg atccctgtct gtaagggtta
    22861 gtttagttgc agccgctgca tccctgacag cttccgctac aggcagaggc tgcccgttca
    22921 ccgtaaagac cctcggtaag ttctttctct gttgcctccc tgatctcaac cacctttccg
    22981 gaaaaattta gctcacaccc ggccagcgga tggttaaagt tcattttcac catatcttct
    23041 agcacttctt ctactatacc gttgagctgg tttccgcggg aatcctgcat agggatcact
    23101 ttatccttta caagaagtcc ttcttccact tttccgttca ctttaaaaat gtcttttgaa
    23161 agttccacga tggcattggg atcaacttca ccgtatccat ccttcgggct caaggtgaac
    23221 gaaaactcgt ccccggtctt tttccccatt atctgttctt caaaggcggg gagaaggtaa
    23281 cctaatccat atataaaacg catgggcttg tcatccttaa ctgtttcgat cacatcaccg
    23341 tcaaccacca gcgtgtatga caatgaaact actttttctt ctgaaatggt catgataaat
    23401 tgttattgtt agtatgtaat tttcttattt tataccctat tgctgcggca aacaatgaaa
    23461 atatcggcgt tattatgttg aagaaacagt agggaaggta cgtcatggtg gccacgttca
    23521 atacagtgga ctgtgccacg gcgcatgtgt tccatggcac gagcacagac gtcacggtgc
    23581 ctgactcttc gacggtgcgg gagagcactt caggcgcaag tccacgctta cggtaagtat
    23641 caccaaacat gttggctggt aaaatgattg ccatatattg atctcccagt gtcatgttga
    23701 aaaataaaca tgtccccaat gttgtgccta tggtagacac ggtgttgcgc atcagtttga
    23761 gtaaattatc agtaattgta ttgatcatgc ccgaggcagt catcacacct gaataggcta
    23821 taacacataa aatgagccaa actgtgttca tcatgccggc catgccgttt gttgatgtaa
    23881 gattgttcag gagctggtct cctgtttcaa cgctgacatg agtgctgagt attttgattg
    23941 ctgcggcaaa acgtcccgga gctatctgat agcaaatatg aggctggaac agtactgcca
    24001 cgatagcccc ggcaatggca gaaaggaaaa gcgtgacaaa cggagatact tttttgacta
    24061 ccaacacaat ggttatgacc gggatgatca ggaaccacgc agaaatgttg aaagtgttat
    24121 ccagtgcggt tagctctgac tgtatgtcaa cggcattatc caccgggatc cagatcgcgg
    24181 caacaacgta gattatcaaa caaataacaa ataccggtcc tgtgttgacc agcatgtacc
    24241 ttatgtgggt aaaaagggga accccgcaga aagatgaggc caggtttgtg gtatctgaca
    24301 ggggtgacat tttgtcgcca aaataagccc ccgatataat cgctccggcg agccacgggg
    24361 tgggaaggcc tatgatttgg cctgccccga gcagggcaac gccaaaagtt cccaccgtgc
    24421 tccatgaact gcccgtaacc aaagatatga gggctgtgat cacaaaggac aggggcagga
    24481 agatggccgg atggatcacc ttgaggccat aatagatcat ggaggggatg atcccgctgg
    24541 tcatccagct cccggtaagg gcccctattg agatcaggat caggatggag gggtatgtat
    24601 ttttcagatg cgagagcatg gattcttccg cctgggacca ggaaatacca tattttaacc
    24661 gtgctatggc aaatgttatg acagcgacaa gcaacagggc aaattgtgaa gggccggcag
    24721 ttaccttgtc cccgaagaca agaacgctgg aaaggagtat ggcgaccaaa agaattacag
    24781 gtatcaggct tactaacagg gatggtttct tcataaatta ttattagagt cctgaaaatt
    24841 caatgtcgtc gaataaaaga gaaggtgttt gccaggacat ggtcttgagt gcatcgtctc
    24901 ctgctgccag gagtctgctc cacaaatcca gcatattgcc tgtcatgacc atttcattga
    24961 tcgggtatgt tatctgtccg tgttccacat aaaaaccttc aatcccgtat gaaaaatcgc
    25021 ctgttgtcgt gttgcagtta cccccgttga atccggtcac aaagatgcca tttttaatca
    25081 aacctgcaag cgttgctgca tttcctttac cttccttcag caacagcagg gaaggagaag
    25141 atatggtagg gtgcatctgc attttttcag cataataggt acttaagaaa tacatattca
    25201 acctgcctgc ttctattacg gcccgttttg cagttgccag accttcactg tcaaagaacc
    25261 ggcagcctgg tgttcctttc ctgcgaggat catcaatgag tgtaagtaca gaggaggcaa
    25321 tattttctcc cagtttatca agaaggaatg aattctgctg ctgcagggag cttcccgaca
    25381 tggcatctat cagaggagcc agcagggtag ccgccaccct gttttctacg atcacccggt
    25441 acctgccgga aactattttt cgggggtttc ttttggccag agccctttca agagctgttg
    25501 cggcacatgt acagggaagt ccggagccta tccctatgtc ccaggggtgg atgcccccct
    25561 gaaaatccca atcggagggc cgggcatcac cttttcccct gacggtacat tcgactgaat
    25621 aattgtacca tgtttcttcc gatgtgcagt gcaaaccgtc tgaatctgcc aggtgtaacc
    25681 actgcctgtg gtctgaatag tctgccataa cacttatcag ggcaggctct ttattccata
    25741 tggcacaggc acaatccagc agaaaggcct tttttacacc ggtgtcgaca gagccactgt
    25801 gggccgaata caagggaggg ccttcatagc gtaattcttt ggggacaagt ttcctgaagg
    25861 ggtccggggc cagcagctta accgaggcaa tggcatcgtc cagcagtttg tccagaccgg
    25921 agggatcaac cttgtttgtt gagcacgtcc cataacgccc ttccacgaaa aggtgcagtg
    25981 tcagggagga atccaccgct ttttcgatgg tttccagaga atcgttcagc acagacatcc
    26041 cgttggcgtt attgcttgtg gcagccacac gggcatgtgt cgcccctttt ttgagagaca
    26101 tctccagggc ataagagcac atttgtttca gaaggtcttt ttgcttcata cacgtcctcc
    26161 caccgtcaaa gaagccacca ggacggatgg catgccgcat gaaaccgcac atgtctggcc
    26221 tttgccgcaa gtccatgtcc cgttgtcgat gatgcaattg ttggccacac ctgttatcct
    26281 ggcaagggct tccggcccgt ttcctattat attggtatcc ttgatcggct gtgtcagttt
    26341 gccgttttct atcaggtatc cgcttttgac atagaaagtg aaatctccgg cccctatctg
    26401 gacctgtccg ttgctgaagg tatccacata aagtcctttc ttgacagaag cgatcagtgc
    26461 ttcttccgtg tccctgccat tgtccatgta agtggcccgc atcctgggca ggggcatgca
    26521 ccggaaagac tccctgcgtc catttcctgt tggttctgta tggaagtagc cggccgagat
    26581 cctgtcatgc aggaaggagg taaggatgcc atcggtgacc atataggttt tctgacccgg
    26641 tactccttcg tcatccacat tgagggcacc cctgttgcac ggaatggtac cgtcgtctat
    26701 gaccgtgatt cccgggttac agatacgttg ccccatttta tcggaaaaga tcgatacttt
    26761 ttttctggca aaatcggctt cgaacgcatg gccaatggct tcatgcaaca ggataccgga
    26821 accaccggcg gccatcacta caggcatttc cccgccattt ggttgttttg ccccgaaaag
    26881 gaatgatgtg cggtcaagga cctcctgtgc cagttcccgg atcatggctg aatgcaagaa
    26941 ttcacctccc atccggaaag agcgggaagc cgaggccgat tccctttgtc ccccggattc
    27001 catgacacag gtgacagaca gggaggccat gggacggata tccgaacaat gaatgcccag
    27061 ggaattgtaa cattcaatat gggatgtggc atcggaaagc tgcgccgtca ctttttcgac
    27121 acgcttgtct gcactgaata tacgttcatt caggtcatag aggaatggca ttttttcctg
    27181 gggcgaaaat ccttcccagg gcctcacgat cctgtaccgg tcgggaaggt tcagagtggc
    27241 aattggcgca ggttttgttc gcctgatgag tccctgcgca atataggatg ccgttctggc
    27301 agcttcttcc ctgtctcgcg gcaatgtgga ttccgtataa gcatagcctg tattctgccc
    27361 gcttaccacc cggatgccca gaccgccttc gttatgcgta gaggccgcat tcacttctcc
    27421 gtcccttaac actatggagt ggaaggtggt ttgttgcata tacaggtcgg cgtaatctcc
    27481 tccctgttga agggccaggt tcagcagttt tttggaatcc atttcaatcg cagttgtcaa
    27541 atccatagcc tggttgctgt ggacatatgt aaacggccgg attccataca gacggggtcc
    27601 agcatgtatg ttgtttcact atgatgggga ttgttcctga aagccggttt tcctgcacgc
    27661 gcagctggaa tgtcacatcg gggatacctg cccattctgc ggggatggta cctgtgaaat
    27721 tgcaattttc cagcctgatg ctcaggcgat cagattttgt tttcatacga ccgaatcctg
    27781 cgggcagggg gccgttgaaa ttagcgttct gctgaaacaa cacagaggtg agattttcca
    27841 gatcgaataa ggcatccggc aggggcaatt ccagtttggt attgaataga cccagacttc
    27901 tgagcacttt caattgaccg atttccgggg gtaaagtccc ggtaaagggt acattgctca
    27961 tttgcaggtt ggtcagtccg gttagttttc ctatgcctgc cgggatgggc cccccaagac
    28021 cggtgcaacc cacaaggttc agatctgtaa gggaagcaag gttgaagagt tcctcaggca
    28081 acccgcccgt aaggcctgtc atggaactga aagaaagacg ctccagggcg gttaggttgc
    28141 ctatccatgc gggaatgttt ccggttatcc cgggatttga agatatgctc agtcttttga
    28201 gcaggacaag ctctcccagg gcctggggga gcgttcctgt aaagttgttg ctgttcaggg
    28261 taaggtgttc cagcgcggtg agttttccaa tggatgcggg tatcgggctc tgtcgggaaa
    28321 tcacgttgga tgccattgac agggaatcca cacgtcctgc cgcattaaga cgaactccct
    28381 cccatgaatc catagtggca gacaggtccc atttgttttt cttccaggtt tctccttcag
    28441 tatccatgaa gaattcaacc agggcaagac tatcctgcat cctgggatcg agcacgaaac
    28501 cagtctgggt caacaagaat tccctgaaaa ggttcagggt ttcatttcca attgtgatga
    28561 cggctgttcg cgtctgcggg tccctgttct ccccgaaaga tatctccagt gagccacttc
    28621 cgttccccga aacaggctgt accaaacacc atgtcctgtc ggagtgggct tcccagggga
    28681 ggtttgccgt aacatggatc gtatagctcc cggccctgta atcatattca gctactgttt
    28741 cccctatgtc cagcaggggc gggattccgg cctgtgtgat ttcaacgctt acggccagca
    28801 ctccggcctt tatggtaaag ttgcccgtga gcggggttcc cagattggga ttttcttcaa
    28861 cgctcaggcg aagggaagcg ctgcctgttc ccgtttccgg gactatgtcg tacccctgtg
    28921 gattctcaag gcgccaggaa gtgttggtat agacaataat atctacccat cctccgcttt
    28981 ggggaaggtt gatcttctct gcagacactg acagttcaat ctcctgtacg gcttccctgc
    29041 accctgtgaa cagcgttaag gataaagcca ggacagccag gcaatgtagg actcctgaaa
    29101 atcgtctcat agaaagcaaa agtacaaaaa aagtattatt tttacatcat agaaagcact
    29161 atggaaaggc cacatcacaa atatccggta ctattggttt tgctcctctt gatgaccgct
    29221 cttccgggaa ggaactctta tgcacaacag cagcagtatg ccctgtcggg tattgtgagg
    29281 gatcagacta ccggtgaacc tgtcccgtat gccactgttt ttctcgaaga tctgggactt
    29341 tggtttattt ccgcggaaga cggctctttc cgcttcaccg ccattcccgc gggaacccat
    29401 acccttcacg ttgaaatcct tgggtacgcc aaaaagacgt tcacacagaa aatcgaacgt
    29461 gacatcacag gcctggtgct tcagatcgcc gaggaaagcc tccggctgga tgaagtggtg
    29521 gtgacagccg aggaaggggg acggcttaac tcctcgtcaa ggattgaaaa acaggcgttg
    29581 gaccacgtgc agccggtaag cctgagggac gtcatgcagc tggttcctgg gaatctgaca
    29641 gaaaatcccg gtctcaaccg ggtgaatcag cttaccatta gggatattgc gtcaaatagc
    29701 gccaatgcct tcggaacagc cgtgatcatg gacggagtca ccctctccaa cgatgccaac
    29761 ctgcaggtga ggaaaacttc aaccattgga acagccggtg aatcgaccgc aggctccggg
    29821 gtggatgcaa gacagatatc taccgacaat atacagactg tggaaatcat aaggggaatt
    29881 ccctctgccg tgtatggaga ccttacatcg ggcgctgtgg tcgtaaaaac ccgctcgggg
    29941 gtttccccgt ggcagatacg cctgaaagcc gatccccgcc tgaagcaggt ctatgccgga
    30001 aaaggttttt ctctcggtcc ggaaaaaggt gtggtgaaca tcgacggcga ctatgccctc
    30061 tcatacggag atgtcaggac accggccgat gtatacagga gggtcaacct gcagataggt
    30121 tattcccaga cagtgagaaa aaaactgacc tataatgccc ggatgcgcct gatatactcc
    30181 gatgccgata acaggactga tccggacaat tttctggaca atatttccag ggacagagat
    30241 atgggcctcg ggttggacat caacggcaca taccgcttga acaagaaatg ggtcaccaac
    30301 ctggaattca tggctgcagg cgatgtaaca tcccagtact cactggagaa aacgtaccgg
    30361 agtcagggga ggataccgca atccctggcc atgacgtccg gcgaaaatct cggctttttc
    30421 acgccatacc gctattattc caacctggaa gtgttcggat tgcccatcaa tgtccaggcc
    30481 agggtggttg cgaacctcta tggaaaatac ggtgacatca ccaataaggt gctggccggt
    30541 gtggaatgga cctccagggg gaacaggggc gcgggcaagg tcttcgatcc ctatttgtct
    30601 cccgatgtca actcaacttc cacatatagg gaacgttcct tcagggatat tcccttcctg
    30661 aaccggtata gtgtttttgc cgaggacaag ctgcgggttc ccctgggcct tgtccgtgcg
    30721 ctggaaattc aggcaggagt ccgatttaac ggcatagtgc cagacacgcg cttcagcatg
    30781 gaccggtatt attctgtcga gccgaggctg aatgcccggt tccagatcct gagacaacag
    30841 gagggattcc gggaactgag cattagggca ggctgg
//
LOCUS       DAOSOF010000004        29839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1559_length_29839_cov_4.437853, whole genome shotgun sequence.
ACCESSION   DAOSOF010000004 DAOSOF010000000
VERSION     DAOSOF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1559_length_29839_cov_4.437853"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1345)
                     /gene="argH"
                     /locus_tag="PLM68_00460"
     CDS             complement(41..1345)
                     /gene="argH"
                     /locus_tag="PLM68_00460"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT53875.1"
                     /translation="METLWNKGIETPGEVTRFTVGNDPHWDMRLAPYDIKGSMAHVRM
                     LGKTGLLTQQETEVIITALDRILEEILQGRLVIGQEAEDIHSLVELALTRELGETGKK
                     IHAGRSRNDQVMTDIHLYLKDVCREIALLTGNLFRCLQQKSESYKDIFMPGYTHERPA
                     MPSTFGLWLGAYAEVLVDDMHQLGAAWEQCDQNPLGSAAGYGNSFPLDREETTRLLGF
                     STLKFNSIAAQMSRGRTERSLSYAMASLAGTLNKLAADCCLFSGPDYGFIEFPESLTT
                     GSSIMPHKKNLDVWELIRAHCNNIQGLPNQITLVCTNLTHGYHRDYQLLKDFLFPAID
                     SLLDCIRMSLLMVDNMKVNPALAEDARYNTVFSVETVNRLVINGMPFRDAYRQVASSL
                     RDGSYKPEKNTRHTHTGSTGNPANEKIRDKMDRALSRILQQD"
     gene            complement(1326..2405)
                     /locus_tag="PLM68_00465"
     CDS             complement(1326..2405)
                     /locus_tag="PLM68_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT53876.1"
                     /translation="MKEMTDLLCRLIGTPSVSGSEEKTAALLADYLQAQPGIRVERMG
                     NNVIARKDSFSAGLPTVLLCSHHDTVRPAASYSRDPFVPVTENDPQGRTRLYGLGSND
                     AGGAVVTMIRAFCGLQELPFNLLLVLAAGEETSDPAGITRVLPALPEISCAIVGEPTG
                     LQAAVGEKGLLVLDGHTWGRSSHAARNDGVNALYAALDDIAVLRNFRFEKESEIMGPI
                     HLQVTQIEAGEQHNIIPDSCHYVVDIRTTDAYTSSGIVELLGKVVSGQLVPRNLSYKA
                     SLTPLGNPLRDAVKKAGLGTYISPTSSDWMRLPCPAIKLGPGDSLRSHTSDEFIYLSE
                     LEQGVETYTKVLRSLTLQHNGNTLE"
     gene            complement(2402..3142)
                     /gene="argB"
                     /locus_tag="PLM68_00470"
     CDS             complement(2402..3142)
                     /gene="argB"
                     /locus_tag="PLM68_00470"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT53877.1"
                     /translation="MYLNIFKIGGNIVDDPKRLDVFLDTFSHCREKKILVHGGGTLAS
                     ELCRQLGIPVEMNQGRRITGPITLRVAVMVYAGWINKNLVASLLARGCRAMGLSGADA
                     GCILADKRKPEPLDYGYVGDLGKESVNVHYIQSILENGLVPVFSPITCDHAGQLLNTN
                     ADTIASALAVAFRGFYMVRLRYVFDREGVCCPGSEKPLSLLSRAQYEKLRDQGTISDG
                     MIPKVENALCALEQGVGEVFIGNTCIRL"
     gene            complement(3133..4110)
                     /locus_tag="PLM68_00475"
     CDS             complement(3133..4110)
                     /locus_tag="PLM68_00475"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HOT53878.1"
                     /translation="MKHFTSVKDIGPLDKAIQEALRIKKDKFTENELGKNLTALLIFF
                     NSSLRTRLSTQKAAENLGMHVIVLDIQQGAWNLETRKGVIMDGDKPEHLLEAIPVMGC
                     YADIIGVRSFARFESKEEDYGEVILNQFIQYSGKPVISLESATRHPLQSFADHITIEE
                     YKTVEKPRVVLTWAPHPKALPQAVPNSFAEWMIAAGYPLTITHPRGYELDERFTQGAT
                     ITYDQQEAFRGADFIYAKNWAAYRDPDYGRILSTDPSWMVDRKKMELTSNAFFMHCLP
                     VRRNMIVTDEVLDSPRSIVISQAANREIAAQVVLRSILKTLQVANPSCT"
     gene            complement(4121..5128)
                     /gene="argC"
                     /locus_tag="PLM68_00480"
     CDS             complement(4121..5128)
                     /gene="argC"
                     /locus_tag="PLM68_00480"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT53879.1"
                     /translation="MSTKINVAIAGGAGYTGGELIRLLLHHPSAHLVSIVSQSGAGMP
                     LGSVHHDLEEETDLTFEEELPHKDIDVLFLCLGHGRSRDYLGRHNTESIGHIIDLSQD
                     FRHTGNQVAGNRRFIYGLPESWQSPGQYATNLRNAGNVANPGCFAACIELALLPLAAG
                     KLLEEAIHVHAITGATGAGGRPAETSHFPYRDNNLAVYKVFTHQHVTEICQTLSASCQ
                     GEIPPLHFVPIRGDFPRGILASVYTPCRLKQEQAVQWYREYYREHPFVHVSDREISMK
                     EVVNTNKCTLHIQKHGNMLHITSILDNLLKGASGTAVHNMNLLAGLPATTGLQLKSIV
                     F"
     gene            complement(5112..6308)
                     /gene="argG"
                     /locus_tag="PLM68_00485"
     CDS             complement(5112..6308)
                     /gene="argG"
                     /locus_tag="PLM68_00485"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT53880.1"
                     /translation="MKSKVVLAYSGGLDTSFCIPYLKQEKKMEVHTLLVNTGGFSPKE
                     VSALSQKALDLGAASHKSIDIRETYYTRCIRYLIFGNVLKNNTYPLSVSSERAFQAMT
                     VADYARRIGAHAIAHGSTGAGNDQVRFDYFIHVMAPEIQLIAPIRELRISRKEEMDYL
                     KNLGFEFSWEKSRYSINQGIWGTSIGGAETLSSLGELPEEAYPVQCTKTGTEVLTIGF
                     SGGEPISLNGIAMDPLQVIEKIHEIAAPYGIGRDIHVGDTIIGLKGRVGFEAAAPMIL
                     IKAHHLLEKHVLGKWQLYWKEQLANWYGMMLHEAQYLDPVMRNIEDFLENTQKRVTGE
                     VSVRLHPRTFTLAGCTSPFDLMNPAFGQYGEGTAAFTGEDVAGFTRIYSLPVKIYQSL
                     VHEHKD"
     gene            complement(6298..6891)
                     /locus_tag="PLM68_00490"
     CDS             complement(6298..6891)
                     /locus_tag="PLM68_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT53881.1"
                     /translation="MNVRILVANASHIGYAERICELMYISALERGTGIARRSPEYIGE
                     KMSSNKAVIALDGKKVVGFSYIETWSHKSFVANSGLIVDHDYRHLGVASRIKKRIFHL
                     SRQLYPQAKIFGITTGAAVMRINSKLGYRPVPFSELTHDEAFWNGCRDCRNYDILMRN
                     DKRMCLCTGMLYDPAEHPKRQTTRKTQPEKEEIIYEK"
     gene            7167..8696
                     /locus_tag="PLM68_00495"
     CDS             7167..8696
                     /locus_tag="PLM68_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT53882.1"
                     /translation="MKKYYICSVLFLLTCGVMHLRAQEADAWTMSRMRLRTHVSFLAS
                     DSLAGRLIGTEGNLIAGEYIADCFADAGLDEYNGTGYFHYFDVKLPSAVPDIPLAVIE
                     GCNVIGVLPGTDPVLKAEFIVIGAHYDHLGKATRGSGEKQSIYNGADDNASGTAVLIE
                     LAGKLQEKGGLARTVVFAAFDGEEQGLLGSKQFLIDSLFPRESIRIMISLDMVGYYRT
                     SGVLKILGTGTLDDARALLPKTKALRVRFIPYEISPFSATDTKPFASAGIPTLHITTG
                     SSSPYHKEEDESEKVDYEGLTAVTNYVQNLVTALGKHTSLTPSGNYAVIHHVPSSLFS
                     WGPAWALGTNRFVHTKGALEGKTAFYASLGADFRFLWKGFLELNPGIGVEYVGARHAP
                     YPGSSYMNQMHMLALGVPLSLRVYFPEFNKLPVGVYAWATTYYRYYLAGQTFDPGFVF
                     TDVFRRHEWGLGLGLGVRASVFQVGFETRWGMTGLFRPGVLQGYNVKNSTQTIRFSYF
                     F"
     gene            8702..9961
                     /locus_tag="PLM68_00500"
     CDS             8702..9961
                     /locus_tag="PLM68_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HOT53883.1"
                     /translation="MKKNVLFLVFLVYPLIAASQPDKEIMRCVEAVSADSIAGYIGTL
                     TGFHTRHNLSSLTGDEKGIGAASRWLYQKVAGWIPLAGGRLSVENVTYTAGGPGTRLD
                     RQVELTNVMAVLKGSDSREILLLAHYDSRVDDNSDSTSFAPGANDNGSGVACLLECIR
                     VMAGIPLQATVRFLFLSGEEHGLLGAACMADMARSQGWNVTAVLNFDMIGNSQAGETG
                     HRDNTRTRVFSAEGTHRELARYIKETALTYADNVEVALVYRNDRYGRGGDHTPFLKAG
                     YTAVRICEFHENYDRTHQLVREENGVLYGDLPAGVDYEYVRKNTGIGIAVIMNLAKAP
                     AAPAGVKMNTRVLSNYTELEWEPSYGPGVVRGYHVMIRETDQSVWQKRIFTEDTRIRI
                     PYSKDNYFFGVCAVGPGGHESLVSVFE"
     gene            complement(9965..10750)
                     /locus_tag="PLM68_00505"
     CDS             complement(9965..10750)
                     /locus_tag="PLM68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT53884.1"
                     /translation="MKLLFTITTLFCFIMTSVAQEKQPGLQQDTLRPFSVKVVDRRGK
                     PLPGLVVSVKRQENELITGQKGRLQFGSRLSLNRQENALITDQKGLAEFEGVSDSDTI
                     IIYLPNIGETHVPCMGTDSIQIMVRSKKRFRIQDREELAHIRANTSNTIDNVPEFLKR
                     HPARNLAELLNGQIPGLFISTGPRGMTANIRGINSINSSTEPLIVIDGMAYDSFAVVN
                     SFLDVHSIQSIQVQKDGALYGVRGANGVIIITTIGAVSNPLAY"
     gene            complement(10763..11479)
                     /locus_tag="PLM68_00510"
     CDS             complement(10763..11479)
                     /locus_tag="PLM68_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53885.1"
                     /translation="MKKIVLTLMLSFFLLPLSAQDQVNDQDKEAIRSFSVKIVDRKGR
                     PMPNLVVSTVREKVTFLTDRKGIVSLEGISDIDSLVVFLPNIGETHIPCAGLDSVQIT
                     VRSKTKYLVQNRKELADLKTNPSNNIDNVPELLKTRPARDLAELLTGVVPGLRITMGP
                     TGLTASIRGINTITGNTEPLIVVDGMTYDTLAEVNGFLDVHSIQSIQVQKDGGMYGMR
                     GSNGVIIITTIGASSNPLSY"
     gene            11558..13177
                     /locus_tag="PLM68_00515"
     CDS             11558..13177
                     /locus_tag="PLM68_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT53886.1"
                     /translation="MIYPTLKHLYSNSIASYADRPSFSLLGEAPVTYADFAQRVEKAR
                     THLLTAGLQKGDKVALLSTSMPNWNACYFATVISGMVAVPILPDFSPDAIVAILEHSE
                     SKALFVSDKLFAKIPVQAKERLDVIIRAVNIKVIDGQKAGTTPMPEAIPEPGPEDLAT
                     IIYTSGTTSSPKGVMLTHRALCNQLHMLISMIEVLPQDVFLSILPLSHTYECSLGMLY
                     PFMQGASVVYLGTPPTPTSLLPALKEVRPTIMLTVPLIMEKIYTRQIFARFTGNKAMK
                     TLYKVRFIRRKLHRIAGKRLYETFGGRLRFFGIGGSKINTATEKFLLDARFPYSIGYG
                     LTETAPLIAGAVHPNTCLQSIGKVMEGISIRLGDKDPDTGVGEIQVKTPCIMEGYYKN
                     PETDKEAFTDDGWFRTKDLAIMDNRGRIFIKGRLSNMIVGSSGENIYPEEIESIINQH
                     ELVTESLVVQRDGKLVALVYMQDLSQKLRDQITEVHADIMNFVNSKVGKMSRITRILE
                     QENGFEKTPTLKIKRYLYSEMRTRKDKKQAV"
     gene            complement(13180..13914)
                     /locus_tag="PLM68_00520"
     CDS             complement(13180..13914)
                     /locus_tag="PLM68_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOT53887.1"
                     /translation="MIKESRTIACYETDAARNLKPYAFMNLAQEMANQDALGLGFGYE
                     QLIEKQTIWVLSRVEVRFVRPPVWKEPVVMETWHKGEDGIFWLRDFLIRNRDETEDLV
                     RATSSWLIIHVNTRRIQRPQHILHASLLQEKATRHAIATPCEKIPVPKNELCYVESKK
                     VCFSDIDFNQHTNNARYIEWAVDCLDTELVTKHSVEMFRINFNAESRLSETLDLFVAE
                     TAPLGRYIEGRTGDKNVFQVQVSFTS"
     gene            complement(13984..15498)
                     /locus_tag="PLM68_00525"
     CDS             complement(13984..15498)
                     /locus_tag="PLM68_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT53888.1"
                     /translation="MKKIFLSIILCSISAFLLSAAEPDIPVPQDAVITISGNRITLES
                     FISQVEKQTEYLFVYSKQDVNVNSVLPIREGKKTVAAFLSEAFGNSNLNYVFENQYIV
                     LTYKAPEYLSVSGTVIDAQTRKPLVFASLYIVGRGISNVTNGEGYFSLKFPPAFANDS
                     LRISFLGYHPQTLALKDLINKGDKLVVPLNFVATKLPAILVTPINAAGLVARAIEKIP
                     DNYPQETMQMTGFYREMIRKGSNYVTLTEAVLDINKSSYSRMYGIDQVGVFKGRGSVD
                     WARIDTVFVKFRGGINSALQIDVAKYPFLGTNVPEINQIYDFTMEEPVQIDGKINHVV
                     AFQQKDNVDDIWFRGKIYIDMKSEAISRIEFNMNVEGRSDATNIFISRRPLGFKAEVL
                     YAAYMVQYKEIAGDWTFDYSRTEIKFDSKWDRKLFKNTYTITSELAITERSDKSIKIP
                     SEQRVRSTDITLDKVTDFQDEGFWDDYNVIEPESGIEQVIARITRQLKRRAQEQ"
     gene            complement(15579..16541)
                     /locus_tag="PLM68_00530"
     CDS             complement(15579..16541)
                     /locus_tag="PLM68_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT53889.1"
                     /translation="MEEMLHKYFADELTPLEKRALLEVVEKDPELRKEFCRIQNLKAV
                     SSLFPASSDAETATRKLAALKRINHRSIYRQVAVYAAAMLVVGFALWGLLQPLTRSLP
                     EEPSAYMIEVTPPGNRTKSFRLSDGTVVWLNAQSVLRYPNKFTGNERRVELTGEAFFE
                     VAENQEMPFIVTTGQYDIRVLGTKFNVFAYKDHNVKTSLVEGSLEVVNPEQEAPPVRI
                     RPYESIVWEQDGWQRSVFEDSEFLLWRDGIYVFNDVPFIEIVNKLELYYNTNIIVNNE
                     TLAQYRFNAKFRQDDGLESILKTMKKIFRFKIDEDRETNTIKIE"
     gene            complement(16611..17150)
                     /locus_tag="PLM68_00535"
     CDS             complement(16611..17150)
                     /locus_tag="PLM68_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT53890.1"
                     /translation="MNWAIPGKEVEISITRERFDGIYIAYFSRLCRFARTYVLSDEAA
                     ENIVQNVFMFLWEKHRIIQIRTNLSAYLYTLVKNKCIDYLRHKKVDKDYRAEQALNLA
                     ALEQLDTGLIPGEDIEETVMEAINNLPPKCREIFILSRVEGKKYSKISQILGISVNTV
                     ENQMGIALRKLRKDLEHLI"
     gene            complement(17128..17580)
                     /gene="rpiB"
                     /locus_tag="PLM68_00540"
     CDS             complement(17128..17580)
                     /gene="rpiB"
                     /locus_tag="PLM68_00540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT53891.1"
                     /translation="MEVIALGSDHAGFHLKENIKEYLAGLGYKVRDFGTGSPDRVDYP
                     DFGIAVGEAVARGECDKGIVVCGTGIGISISANKVKGIRAAACSDPFSARLSREHNNA
                     NILALGSRIVAPEYARYIVKEWLEAKFQEGRHAERALKIAQYELGNPG"
     gene            17634..18335
                     /locus_tag="PLM68_00545"
     CDS             17634..18335
                     /locus_tag="PLM68_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT53892.1"
                     /translation="MKTYRIEALIIAIALVIMGLLLKHGIDGFSGRDRTVNVKGLSEM
                     EVPADKVTWPLVYKEIGNNLQDLYVRINRNNKTLVDYLISNGISEEEIGINAPEIIDM
                     SAERYSTTPAPYRYNVTSVITVSSTKVDLVRKLIADQGELLKQGIAITGGEYRYNTVY
                     SFTGLNDIKPQMIEEATRNARAAAMKFAQDSGSKLGKIKNASQGQFTISDRDENTPYI
                     KNVRVVTTVNYYLKD"
     gene            18340..18744
                     /locus_tag="PLM68_00550"
     CDS             18340..18744
                     /locus_tag="PLM68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT53893.1"
                     /translation="MNQRKIVETTLAPPAIGPYSQAVIAQGFLYMSGQLGIDPATGAM
                     VESGREEQFEQIFKNAGHILEAAGCGFKDVVKCTGFLTDMDDFPLFNKVYSRYFQLPY
                     PARSVFEVSALPKKGAFAELELVAKMTDNLSF"
     gene            18847..19281
                     /locus_tag="PLM68_00555"
     CDS             18847..19281
                     /locus_tag="PLM68_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT53894.1"
                     /translation="MLPALRHTQNWWPSLFNEFFDNEGLVRFVGTTSPAINVIENEKD
                     YQVEVAAPGMSKEDFCIRLDQENRLTICLEKKNDDSQKDKNGKYLRREFSYSHYEQAF
                     LLPDDIIKDKIAASMENGVLRISIPKDLETPAVPKEYTIEIR"
     gene            complement(20051..21283)
                     /locus_tag="PLM68_00560"
     CDS             complement(20051..21283)
                     /locus_tag="PLM68_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT53895.1"
                     /translation="MSQKGIRNYSKIVVFALASSIFPVLASCERNMDMPEREPVHFRE
                     VLAQDTFAFKDLLSSLVSIYPNFSNQYGGQLEQLMSLLDTTGKVHSTAISYLTKDPNG
                     NEILASGIVLRPLDRESRGLLHFFPSAKIDKTTTGSELMITFEGILSFFGYTVIVPDM
                     IGYGVSSDREYPFLFADNTGQVAYDMHLAAAEYFQSIGLPFPRKVTIGGYSMGGMGVV
                     ALHRHIEQYGTDGFVVEKSYPGGGVYDLGMAVDILTKTRYSTFLFIPYMYVAMNYWYG
                     LNIDYSEVFIDPLLSNMDDWLSRKYLAVEMAQFLSPDITTYMHPDMFTPEKNASFNKV
                     DSCLRLHSVIEKWAPRAPMTIIHTVNDNMAPFEIAEKMYNNFLEKGGNVNLIRADDNH
                     FDSGIAYYISMMLYMLIK"
     gene            complement(21309..21671)
                     /locus_tag="PLM68_00565"
     CDS             complement(21309..21671)
                     /locus_tag="PLM68_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HOT53896.1"
                     /translation="MKDSICMMKDVLMAFNEFEKSLIQAHSLSLNEAMVLCLLGECEK
                     EPGRLSASAIAEKTGFSASHTSKVLRSAESRKWIIRSLCSEDRRKMYFELTPEGQKKL
                     WQIKNTPVEIPALLKKLF"
     gene            complement(21680..24175)
                     /locus_tag="PLM68_00570"
     CDS             complement(21680..24175)
                     /locus_tag="PLM68_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT53897.1"
                     /translation="MKHVIIGGVAGGATTAARIRRNDENAEIIMFEKGKYISYANCGL
                     PYYIGDVIKDRAKLFVQTPESFGKRFNVDVRVNSEVIGIDTEKQLVTVRNAKGDEYRE
                     SYDKLLLSPGANPVKPPLPGIDSEGIFTLRNVDDTDRIKEYMRSHPIRRAVVVGAGFI
                     GLEMAENLHATGAHVAIVEMAEQVMTPIDFSMAGLVHQHLYQKHVDLHLNQAVQGFIP
                     VSGPDPSIKINLKNGNELEADIVILSIGVRPDTTLAKQAGITLGRTGGIQVNEYLRTS
                     AENVYAIGDAIEFPHPVSGEPWLNYLAGPANRQARIVADNMVSGDRIKYEGSIGTSIA
                     RVFDLTVASTGLPGKKLKQLNIPYLTSTTHSNSHAGYYPNALQMSTKITFDPETGRLY
                     GGQIVGYDGVDKRIDLLAHVVKKQGSIYDLITMEHAYAPPFSSAKDPVAIAGYVASNI
                     LSGKMKPVYWRKMQKTGPGEVTKLDVRTAAENSLGTISGSVNIPVDELRNRLDEVPRD
                     KPVYVFCAVGLRGYLASNILIQNGFNNVYNLSGGYKTYEAAAESEKKSIPITPAFSAP
                     YSDSGDPLAKNKAAASSKAATIEVNTLKIDATGLQCPGPIMKIKSSIDSIVPGQRVEI
                     QATDPGFARDIQAWCQTTGNVLVQQSDSKGVFTAVVEKKERGIQAPAAPEGGKGKTLI
                     MFSDDLDKALATFVLANGAAATGEKVSIFFTFWGLNVIKKIKKPAVKKDIFGKMFGMM
                     LPSHSGALKLSKMNMFGMGRIMMRHIMKIRGIDSLEDLRRQAIENGVEFIACQMSMDV
                     MGVKAEELMDNVIIGGVATYMNRAVQSNVNLFI"
     gene            24389..25672
                     /locus_tag="PLM68_00575"
     CDS             24389..25672
                     /locus_tag="PLM68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT53898.1"
                     /translation="MFVHFGPNTFTGVEWGNGAEDPAVFNPRALDCRQWARTAKNAGM
                     QGIIITAKHHDGFCLWPSRYSDHTVRNSPWRSGQGDVLAELSAACREYGLKFGVYLSP
                     WDRNNPAYGTPEYNRLFANTLTEVLTSYGPVFEQWFDGACGDLVSSPYDWSLFNSTVQ
                     EHQPGALIFSDVGPGCRWVGNERGFAGETNWSTLDTSGFTPGRGAPSTIILNSGQRGA
                     PYWIPVEADVSLRPGWFYNPDTDNSLKSVEELMDIYFGSVGRNANLLLNVPPDTRGRI
                     PAGDSLRLMEFRQRRDEVFSRDLARGDAAFATSVRNDGSRKFRPDNVTDADYDTYWIP
                     EEGITTAALTIDLGKEENFSALMLQEYIPLGQRIARFIVEYADPEEDRWLLLAQGTTI
                     GYKRLFRFPAVTSDKIRISILEADAPPLLNAIAIY"
     gene            25696..26817
                     /locus_tag="PLM68_00580"
     CDS             25696..26817
                     /locus_tag="PLM68_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT53899.1"
                     /translation="MNKLAKYIIAVAALAIIVFIVWYFRSIITYILIAALLSLVGSPL
                     VRLLSRIRIKKWHIPKSVSSILVICAMFGLIFLFFYYMAPLVIGIASRIDTIGLEDVP
                     AMLSGSLAQANKLIHGLFPSLAPSMTIESLIWNEIEKFLHPEFFTRLFDSLASFFVNF
                     GVGLFSVVFITFFFLREENMFSNMLMAIVPVKYEERFSNAMKSVNELLLRYFIGIFIE
                     VLIMTILISAGLHFVVRIEYKLALVLGLIFGILNIVPYAGPLIAGSIGTVIGILTSYA
                     TSGYSSILWFVVAMVAVYAGINMLDTYLIQPVIYSSSIKAHPLEIFLVILISGYMGGA
                     VGMLVAIPAYTVIRVFASEFLSRFKVVQKLTAKLPDHGD"
     gene            26807..27580
                     /gene="zupT"
                     /locus_tag="PLM68_00585"
     CDS             26807..27580
                     /gene="zupT"
                     /locus_tag="PLM68_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT53900.1"
                     /translation="MEIEPSQLLIAFVLTLFAGLSTGIGSLAALLAKRTNTSFLSVTL
                     GFSAGVMVYISFMELIPGARQILTPLQVVLSFFGGIALIAIIDFLIPKDENPHELHLT
                     EDMDKKSRLKRMGIMIALGIAIHNFPEGIAAFIAGMKSLDVGIPIALAIAIHNIPEGI
                     AVSVPIFYATGNRKKAFWYSLLSGLAEPFGALVAILILFQYWDPLLEGIVMTAVSGIM
                     VYISTDELLPGAERFGKHHLSILGFIGGMAIMASSLLLI"
     gene            27601..28482
                     /locus_tag="PLM68_00590"
     CDS             27601..28482
                     /locus_tag="PLM68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT53901.1"
                     /translation="MSNVVLNEIPPLSDKDCFHVVERYKSEFTFPLHQHREYEINFIE
                     HGKGLKRIVGDNIEYTDDYDLILISGDSLEHVWDQGNCKSPVIREITIQFSPDLLPTC
                     LLEKNQFNSIRKMLIKAEHGLAFPLETIMKVYNLIDTIAREGKGFEQFLRLLKLLNEL
                     SLCVNSRSLASSSFAHAERNMESRRVSKVKDYINQNYTKKLSLDLLSEMVGMSPVAFS
                     RFFKLRTGKTLSDYIIDIRLGYASRMLVDSNRTISEICFECGFNNLSNFNRIFKKKKH
                     MTPKLFRAHYRKSKSTI"
     gene            complement(28445..>29839)
                     /locus_tag="PLM68_00595"
     CDS             complement(28445..>29839)
                     /locus_tag="PLM68_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53902.1"
                     /translation="SFTRRPTLVSKGDLIFILLLSELKKGTSATLEFNLSARGLIDKE
                     PVTIRADFTSPGRRFDGFGGNFRLQNPQMDPKVIQYCLDNMRVAWGRVEMPWANWQPF
                     EDKAPAPDQRVQDAMEMARRLSARGMPVIVSAWFPPAWAVQGDISEYWRRGGGVRAYR
                     LDPEKKEAIYNSLADYLDYLKKWYGVEAYAFSFNESDIGIDVFHTPREHAVFIRELGQ
                     VLASRGLATKILLGDNSDATTFDFILPAMEDLSTHKYIAAVSFHSWRGCDQATLEKWA
                     GAARRLNVPLIIGEGSTDAAAHRYPAIFAESTFAFYEINLYIRICAICQPLSILQWQL
                     TSDYSILWGDGIYGSSGLLRPTQRFWNLKQLASTPENVFSMPVQSDRDLINCAAFGNP
                     GTGDYAIHIVNNGAVREATLEGIPAGKAVVYVTNENDGMKEIEKITTTGEAFMLTLPA
                     LSFISLICSSGNGP"
BASE COUNT         7252 a         7238 c         7330 g         8019 t
ORIGIN      
        1 gagatcatct ggtacatcag gaacatgtcc tgatacctgt tcagtcctgt tgcaatatcc
       61 gggagagggc cctgtccatt ttgtcccgga ttttttcatt tgcaggattg cccgtgctgc
      121 ccgtatgggt atgacgtgta ttcttttccg gcttgtagga accgtcacgg agagacgagg
      181 ctacctgccg gtaggcatcc ctgaacggca tgccattgat gaccagacgg tttacagttt
      241 ctacactaaa aaccgtatta tatcgggcat cttctgctag cgcagggttg actttcatgt
      301 tgtccaccat caggagagac atccggatac aatcgagcag cgaatctatg gccggaaaaa
      361 gaaagtcttt aagcagctgg taatccctgt ggtacccatg ggtcaggttg gtgcacacca
      421 gggtgatctg gttgggcagg ccctggatat tgttgcaatg cgccctgatc agttcccaga
      481 catcgagatt ctttttgtgc ggcatgatac tgcttcccgt ggtcagtgat tctggaaatt
      541 ctataaaacc atagtcagga cctgaaaaca ggcaacaatc tgcagcaagc ttgttcaggg
      601 ttccggcaag agaagccatg gcataagaaa gggaacgctc tgttcttccc cggctcatct
      661 gtgctgcaat actgttgaat ttcaaggtgc tgaatcccaa aagccttgtt gtttcctccc
      721 tgtccagcgg gaatgaattc ccgtatccgg cggctgaacc cagaggattc tggtcacatt
      781 gctcccatgc cgcgcccagc tggtgcatgt cgtctaccag cacctcggca taagctccca
      841 gccaaaggcc gaaagtagaa ggcatggccg gtctttcatg ggtgtacccg ggcatgaaga
      901 tatctttgta cgactcgctt ttctgctgca gacaccggaa aagattcccg gtaagaaggg
      961 caatttccct gcagacatcc ttaaggtaca ggtgaatgtc ggtcatcacc tggtcattgc
     1021 gggaacgtcc cgcatgtatt tttttgcccg tctcacccaa ctctcgcgtc aatgcaagct
     1081 cgaccaggga atgaatatcc tcggcttcct gcccgatgac cagtcttccc tgcaggattt
     1141 cctccaggat cctgtcaagg gcggtgatga ttacttctgt ctcctgttgc gtcagcaggc
     1201 cggtcttgcc cagcatcctg acgtgtgcca tggatccttt tatgtcatag ggagccagcc
     1261 gcatgtccca atgaggatcg tttcccacgg tgaaccgggt gacttccccg ggcgtttcaa
     1321 ttcctttatt ccagagtgtt tccattatgt tgcaatgtta gtgaccggag cactttcgtg
     1381 taggtttcca ctccctgctc cagttcagat aagtaaatga attcgtcgga cgtatgggac
     1441 ctgagagagt ctccgggacc cagttttatg gcagggcagg gcaggcgcat ccagtccgac
     1501 gaggtagggg atatatatgt gccgagtccc gcttttttca ccgcatcgcg caacggatta
     1561 cccaatgggg taagggaggc tttgtatgaa agattccgtg ggacgagctg tcccgagaca
     1621 accttgccca gcaactccac tatcccgctg ctggtataag catctgttgt tcttatatcc
     1681 accacataat gacatgagtc gggaatgatg ttgtgttgtt ccccggcttc aatctgtgtc
     1741 acctgcaggt gaatcggacc catgatttcg gattctttct caaaacgaaa atttcttagg
     1801 actgcaatgt catccagggc tgcataaagc gcgttcaccc cgtcgttacg ggcagcatgg
     1861 gaagagcgtc cccatgtatg tccgtccagc accagcagtc ccttttctcc cacggcggcc
     1921 tgtagcccgg tgggctcacc cactatggcg cagctgattt cgggaagagc tggcaatacc
     1981 cgggtaatac ccgccgggtc cgaggtttcc tctcctgctg caaggaccag caaaagattg
     2041 aagggcagct cctgcaggcc acagaaagcc cggatcatgg ttaccaccgc gccccctgca
     2101 tcgttgcttc ccagaccgta cagccgtgtc ctgccctgag gatcgttttc agtcacaggg
     2161 acaaaagggt ccctggaata actggcagcc ggcctgaccg tgtcgtgatg ggaacaaagc
     2221 agaaccgtgg gaagtccggc tgaaaaagaa tctttccggg ctatgacatt attgcccata
     2281 cgttctaccc tgatgcccgg ttgtgcctgc agataatctg caagtaacgc ggctgttttt
     2341 tcctcactgc ccgaaacaga tggcgttcct atcagcctgc agagcagatc agtcatttct
     2401 ttcataaccg tatacaggta ttacctatga aaacctcacc cacaccctgt tccagggcgc
     2461 atagcgcatt ttccaccttg gggatcatcc cgtccgagat ggtaccctga tcgcggagtt
     2521 tttcgtattg agcacggctc agcagggaca gcggcttttc cgaaccggga cagcaaactc
     2581 cttccctgtc gaaaacatac cgcagcctga ccatatagaa acctctgaat gcaacagcca
     2641 gggccgaggc tatggtatcg gcgttggtgt tgagcagttg tcccgcatgg tcgcaggtga
     2701 tcggtgaaaa gaccggtacc aggccatttt ccaggatact ttgtatgtaa tgcacattta
     2761 cgctttcttt tcccaggtcc cccacataac cataatccag cggttccggt ttccttttat
     2821 cagccagaat gcagcctgcg tccgccccgc tcaggcccat cgcgcggcag cccctggcaa
     2881 gtaacgaggc caccaggttc ttgtttatcc acccggcata caccatgaca gcaacacgca
     2941 gggttatggg accagtgatc ctccgcccct ggttcatttc aacgggtatg cccagctgcc
     3001 ggcacagttc actggccagg gtgcctccgc cgtgaacaag tatctttttt tcccggcaat
     3061 gggaaaaagt gtccaggaat acatccagcc ttttggggtc atctacgatg ttgcctccaa
     3121 tcttgaaaat attcaggtac atgatggatt tgctacttga agtgttttca atatactgcg
     3181 aagtaccacc tgggcggcga tctcacggtt ggctgcctgg gaaatgacaa tggagcgcgg
     3241 actgtccagc acctcatccg ttacaatcat gttgcgccta actggaaggc aatgcatgaa
     3301 gaaagcattg ctggtgagtt ccattttctt cctgtccacc atccaggagg ggtctgtgct
     3361 cagtatcctg ccatagtcgg ggtccctgta ggcagcccag ttcttggcgt agatgaaatc
     3421 tgccccgcgg aaagcctcct gctggtcgta tgtgattgtt gcgccctgtg taaagcgctc
     3481 atccagctca taacccctgg gatgggttat ggtcagcgga tatccggcag ctatcatcca
     3541 ttcggcaaat gagttgggta cggcctgggg gagggccttt ggatgcggtg cccaggtcag
     3601 gaccacccgt ggtttctcta ccgtcttgta ctcctctatg gtaatgtggt ctgcaaagct
     3661 ttgcagcgga tggcgggtag cggattccag gctgatgacc ggtttcccgg aatactggat
     3721 gaactggttc aggataacct caccgtaatc ctcttctttg ctttcaaaac gggcaaagga
     3781 acgtacccct atgatatctg cataacatcc catgacggga atggcttcca gcaggtgttc
     3841 cggcttgtcc ccgtccatga tgacaccttt gcgtgtttcc aggttccagg cgccttgctg
     3901 tatgtccagg acaatgacat gcatgcccag gttttcggct gctttctgtg tgctcaggcg
     3961 ggtacgcagg ctggaattga aaaaaatcaa cagggccgta aggtttttcc ccagttcgtt
     4021 ttctgtgaat ttatcctttt tgatacgtaa tgcttcctgt atggctttgt caagtggacc
     4081 gatgtccttg accgaggtga aatgtttcat ggccgtcttt ttaaaatacg attgatttga
     4141 gctgcaatcc tgttgtcgcg ggcaaccccg caagcaggtt catgttatgt actgccgttc
     4201 ccgatgcccc ttttagcagg ttgtccagaa tggaggttat gtgcagcatg tttccgtgtt
     4261 tttggatgtg cagtgtgcat ttattggtat tgaccacttc tttcatgctg atctcccggt
     4321 cagagacatg gacgaacggg tgttcccggt aatattccct gtaccattgc actgcctgct
     4381 cctgtttcag gcggcagggc gtatagacac tggcaaggat cccccgggga aagtcacccc
     4441 ggatgggaac aaaatgcagg ggcggtatct caccctggca ggacgcagat agcgtctggc
     4501 aaatttccgt gacatgctgg tgggtgaaaa ccttataaac ggccaggttg ttatccctgt
     4561 atggaaaatg tgacgtttcg gcaggccgcc ctccggctcc tgttgcccct gttatagcgt
     4621 gcacatgaat cgcctcttcc agcagttttc ctgctgccag aggtaacagg gcaagctcga
     4681 tacaggcggc aaaacacccc gggttagcca cattgcctgc attcctgagg ttggtggcat
     4741 attgcccggg agactgccag gattctggca atccgtagat gaaacgacgg ttgcctgcca
     4801 cctggttccc cgtatgcctg aaatcctggc tcaggtcaat gatatgaccg atagattcag
     4861 tgttgtgccg acctaggtaa tccctggaac gtccgtgtcc caggcacagg aacaacacat
     4921 caatgtcctt gtggggaagc tcctcctcaa aggtgagatc cgtttcttct tccaggtcat
     4981 ggtgtaccga gcccaggggc attcctgccc cggattggct tactatagaa acaagatgcg
     5041 ccgaagggtg atgcaacagc aaacggatga gttccccgcc ggtgtatccg gcgcctccgg
     5101 ctatggctac gttaatcttt gtgctcatga acaagacttt gataaatttt caccgggagt
     5161 gaataaatgc gtgtaaaacc tgccacatct tccccggtga atgccgcggt tccttctccg
     5221 tactgtccga aggccgggtt catcagatcg aacgggcttg tgcaacctgc cagggtaaac
     5281 gttcggggat ggaggcggac cgatacttcc ccggttacac gtttctgggt gttttccagg
     5341 aaatcttcta tgtttcgcat cacggggtcc aggtattggg cctcgtgcag catcatgccg
     5401 taccagttgg caagttgctc tttccagtac aactgccatt ttcccaggac atgcttttca
     5461 agcaggtggt gagcctttat caggatcatg ggtgctgccg cttcaaaacc cacacgtccc
     5521 ttcaggccta tgatggtgtc tcccacgtgg atgtccctgc caatcccgta aggagcggct
     5581 atttcatgta ttttttcaat gacctggagg ggatccatgg ctatcccgtt cagtgaaatg
     5641 ggttctcctc cggaaaatcc aatggtcagt acttcggtac cagttttggt gcattgcacc
     5701 gggtaggctt cttcaggcaa ctcacccagg gagctgagcg tttcggcgcc cccgatgctt
     5761 gttccccata ttccctggtt gatagaatac ctcgactttt cccagctaaa ctcaaaaccc
     5821 aggttcttca ggtaatccat ttcttccttc ctgctgatcc ttagttccct gataggggca
     5881 attagttgta tttccggtgc catcacgtgt atgaaataat caaaacgaac ctggtcgttg
     5941 cccgcacccg tactgccatg agcaatggca tgggctccga tgcggcgggc gtagtctgcc
     6001 accgtcatgg cctggaatgc acgctcagaa cttaccgaca agggataggt gttgtttttc
     6061 aacacatttc caaagatcag atagcgaata caacgtgtat aataagtttc tcttatgtca
     6121 atggatttgt gggatgctgc tcccaggtca agggcttttt gtgacaatgc actgacctct
     6181 ttcgggctga aaccgcccgt attcacaagc agggtatgga cctccatctt tttttcctgt
     6241 ttcagataag gaatacagaa agaggtgtcc agtccgccgc tgtaagccaa tactacttta
     6301 cttttcatag ataatctctt ctttttctgg ttgtgttttc ctggttgttt gccgtttcgg
     6361 gtgctctgcc ggatcgtata gcatccctgt acagaggcac atgcgtttgt cattgcgcat
     6421 gaggatgtca taattgcggc agtccctgca tccgttccag aatgcttcat cgtgtgtgag
     6481 ctcggagaag gggacgggcc ggtatccgag ttttgaattg atcctcatga ccgcagcacc
     6541 tgttgtgatc ccgaaaatct ttgcctgggg atacagctga cgcgatagat ggaagatcct
     6601 ttttttgatt ctggaggcaa cgcccaagtg cctgtagtca tgatccacta tgagacctga
     6661 attggccacg aatgatttgt ggctccaggt ttctatatag gagaatccta caactttttt
     6721 gccgtcaagt gcgatcacgg ctttattgct gctcattttc tcaccaatgt attccgggga
     6781 acgccgtgca ataccggttc cgcgctccag ggcggagatg tacatcagtt cacaaatacg
     6841 ttctgcatag ccaatatgag aggcatttgc aacaagaatg cggacattca ttgtcttttt
     6901 taataatgat tatggaatgt ttctggaaaa tttaaaaagg gcccgtcagg atgcagaaat
     6961 gaattctgca gcctaagccc gtcgtcggat acgtagaatc tgaaggatct ttttcattca
     7021 gcggcaaaag taatgtaatt tgcataatta ggacaagatt tttattaatt atttcaaata
     7081 gttttttttt aatcgtatac actttcattt tgtaagtaga aaagcttttc atcacaaata
     7141 cttatatttg cattgactca ggcactatga aaaaatacta tatctgttct gtactttttc
     7201 ttctcacatg cggtgtgatg catctccggg cgcaggaagc cgatgcctgg accatgtcca
     7261 ggatgagact gcgaacacat gtctccttcc tggcttccga ttcgcttgcc ggaaggctga
     7321 ttggcacaga aggcaatctg attgcaggag aatacatagc tgactgtttt gcagatgcag
     7381 gtcttgacga atacaacggc acaggttatt ttcattattt tgatgtgaaa ctcccctcgg
     7441 ccgttccaga tattccgctt gctgtcatag aggggtgtaa tgtaattggg gtcttgccgg
     7501 gaacggatcc cgtcctgaaa gccgaattca tagtcatcgg ggctcactac gatcacctcg
     7561 gaaaggcgac caggggttcc ggggagaagc aatccatata caatggagcg gacgacaatg
     7621 cttctggaac agctgtcctg atagaactgg ccggaaagct tcaggaaaag gggggattgg
     7681 caaggacggt tgtgttcgca gcttttgacg gagaagaaca aggcttgctc ggctcgaaac
     7741 aattcctgat tgattccctg ttcccgcggg aaagtatacg aatcatgata agccttgata
     7801 tggtgggata ttacaggaca tcaggcgttc tgaaaatact gggcactggc acgctggatg
     7861 atgcgcgtgc tttattgccc aaaaccaaag cgctgagggt tcgtttcatt ccctatgaaa
     7921 tttctccctt ttcagcaaca gatacaaaac cttttgccag cgcgggaatc cctaccttac
     7981 acattaccac gggaagcagc tctccttatc acaaagagga agatgagtct gaaaaagttg
     8041 attacgaggg acttactgcc gttacaaatt atgtccaaaa cctggttact gccctgggaa
     8101 agcatacttc cctgactccg tcaggtaatt acgcggtgat ccaccatgtt ccctcgagcc
     8161 tgttcagttg gggacctgca tgggccctgg gtaccaaccg ctttgtacat acgaaggggg
     8221 cactggaagg gaagactgcc ttttatgcat cactgggtgc ggatttcaga ttcctgtgga
     8281 aaggtttcct ggagctgaat ccgggtatcg gggtggaata tgtgggggca cgccatgcac
     8341 cgtatcccgg gtcgtcttat atgaaccaga tgcatatgct tgccctgggc gtaccgttat
     8401 cactgcgggt atattttccc gaattcaata aactgcctgt aggtgtttat gcctgggcaa
     8461 ccacgtatta tagatattat ctggccggac agacctttga tcccggtttt gtttttaccg
     8521 atgtattcag acgccacgaa tggggacttg ggctgggatt gggggtacgt gcttctgtgt
     8581 tccaggtggg attcgagacc aggtggggga tgacaggcct tttccggccc ggagtgcttc
     8641 agggatataa tgtgaaaaac agcacccaaa cgatccggtt ctcttatttc ttctgatggt
     8701 tatgaaaaag aacgtactct ttttagtgtt tcttgtttac ccgttgattg cagcctcaca
     8761 gccggacaag gaaatcatgc gttgtgttga agcagtttct gcagacagca tagccggtta
     8821 tatcgggaca ctcaccggtt ttcatacgcg ccacaacctg agttccctga ccggtgatga
     8881 aaagggaatt ggtgctgctt cccggtggtt gtatcaaaag gttgccggat ggatacctct
     8941 ggccggagga agactttccg tggaaaatgt tacttatacg gccgggggac ccgggacaag
     9001 gctggacagg caggtggagc taaccaatgt catggctgtg ctgaaggggt cagacagccg
     9061 tgagatcctt ctcctggcgc attatgacag tcgtgtggat gacaacagcg actccacatc
     9121 ttttgccccg ggagcgaatg acaacgggag cggggtagcc tgcctgctgg aatgtatccg
     9181 ggtcatggcc ggcatcccct tgcaggcaac tgtgcgtttc ttgttccttt caggagagga
     9241 gcatggcctg ttgggagctg cctgcatggc cgacatggcc cgttcacagg gatggaatgt
     9301 cacggctgtt ctaaattttg atatgattgg caattcgcag gcaggggaaa ccggtcatcg
     9361 tgacaacacc aggacaaggg ttttctcggc agaaggaact cacagggaat tggcaagata
     9421 tatcaaggaa accgcgctga catacgcaga caatgtggag gtggcgctgg tctacaggaa
     9481 tgacaggtat gggcggggtg gtgaccacac acctttcctt aaggccgggt atactgctgt
     9541 caggatttgt gagtttcatg agaattacga caggacgcac cagctggttc gggaggaaaa
     9601 cggggtcctg tatggagatc ttcctgcagg tgttgattat gagtacgttc gtaaaaacac
     9661 cggcattggt atagctgtca tcatgaacct ggccaaagct cctgcggcac ctgccggcgt
     9721 gaagatgaac acgagggtgt taagcaatta cacggaactg gagtgggaac cgtcttacgg
     9781 acccggtgtg gttaggggat accacgtaat gatacgtgaa acagaccagt ctgtatggca
     9841 aaaaagaatt ttcacagagg acacacggat ccggattccc tacagcaaag acaattattt
     9901 tttcggggtc tgtgccgttg gccccggcgg tcatgaaagc ctggtatctg ttttcgaatg
     9961 attatcagta ggccagggga tttgagacag cgccaatggt agtgattatg atcaccccgt
    10021 tggctccacg gaccccgtac agcgctccgt ccttttgtac ctggattgac tgtatgctgt
    10081 gaacgtcaag gaacgagttg accacggcaa aagaatcgta ggccattcca tcaattacta
    10141 tgagaggttc tgtgctgctg ttgatggaat tgatgccccg gatgttggct gtcattcccc
    10201 ggggacctgt agaaatgaac aagccgggta tctggccgtt tagaagctct gccaggttcc
    10261 ttgccggatg acgtttgaga aattccggaa cgttgtcgat tgtattggag gtattggcac
    10321 gaatgtgtgc cagttcctcc ctgtcctgaa tgcggaaacg ttttttagac cgcaccatga
    10381 tctggatgga gtctgttccc atgcagggaa cgtgggtttc accaatattt ggcaaataga
    10441 ttatgatggt atctgaatcg gaaacacctt caaattctgc cagacctttc tggtcggtta
    10501 tcaaagcatt ctcctgtcta ttcaatgaga gcctcgaacc aaactgcaat ctgcctttct
    10561 ggccggtaat caactcattc tcctgtcttt tgactgaaac cacaagcccg ggcaagggct
    10621 tgcccctcct gtcaactacc ttgacgctga aagggcgcag ggtgtcctgc tgcaatcctg
    10681 gctgtttctc ctgtgcaaca gaagtcatga taaaacaaaa cagggttgtt atggtaaaca
    10741 ataatttcat gacgatttca tattagtatg atagaggatt agaagaggcg ccaatggtgg
    10801 taataatgat aaccccattg gagccacgca taccgtacat acctccgtcc ttctgtacct
    10861 gtatggactg gatgctgtga acatcaagga atccgttcac ttccgccaaa gtatcataag
    10921 tcatcccgtc aaccacaata agtggctctg tgttgcctgt aatggtgttg atcccgcgta
    10981 tgctggctgt cagcccggta ggtcccatgg ttatgcgaag tcccggtacc actcctgtca
    11041 gcagttctgc caggtcccgt gcaggccgcg tcttgagcaa ttccggaaca ttatcgatgt
    11101 tgtttgaagg atttgttttc agatctgcca gctcttttct gttctggacc aggtactttg
    11161 tttttgacct gaccgttatc tgaacagaat ccagaccggc acaggggatg tgtgtctctc
    11221 cgatgttggg caggaatacc accagggagt cgatatcgga aattccttcc aggctgacta
    11281 tgcctttcct gtctgtgagg aaagtcactt tttccctgac agtggatacc accaggttgg
    11341 gcataggcct tcctttcctg tccactatct tgacgctgaa agagcggatc gcttccttgt
    11401 cttgatcatt tacctgatcc tgtgccgaca acggcagcag gaaaaatgat agcatgaggg
    11461 tgaggactat ttttttcata atttcaaata taacaaaaat tatacctttg tcggcattat
    11521 tattgcacat aagcaatgaa ttcatagata aaccagaatg atttatccga cattaaaaca
    11581 cctatattca aatagcatag caagttacgc ggaccgtccc tctttttccc ttttagggga
    11641 agcaccggta acctatgcag attttgccca aagagtagaa aaggcaagga cgcatctgct
    11701 cacggcggga ttacagaaag gggacaaagt ggccctgctt tccaccagca tgcccaattg
    11761 gaatgcatgc tattttgcca ctgtgatatc gggaatggtg gctgttccca tactgccgga
    11821 tttttccccc gatgcgatag ttgccatact ggaacattct gaatcaaaag cactctttgt
    11881 atcggacaag ctttttgcaa aaatcccagt tcaggccaaa gaacgtcttg atgtcatcat
    11941 cagggcggtc aacataaagg tcatagacgg ccaaaaagca ggtacaacgc ccatgccgga
    12001 agcaattcca gaacccggtc ctgaagacct ggccacgatt atatacacct cggggaccac
    12061 ttcttctccc aaaggggtga tgctgacaca tcgtgcgctg tgcaaccagt tgcatatgct
    12121 gatttccatg attgaagtgc ttccccagga tgtattcctt tcgatactgc ctctttcaca
    12181 tacgtatgaa tgttccctgg ggatgttgta tccctttatg cagggagctt ccgtagtgta
    12241 tctggggacc ccgccgacac ctaccagcct gctgcctgcc ctgaaagagg tacggcctac
    12301 gatcatgctg acggttcccc ttatcatgga aaaaatctat acaaggcaga tttttgcccg
    12361 ttttaccggg aacaaggcaa tgaagactct ctacaaggtg cgttttatcc gtcgtaagct
    12421 tcatagaatt gcaggaaaac gcctgtatga aactttcgga ggaaggctgc gtttctttgg
    12481 catagggggt tcaaagatca atacagccac agaaaaattc ctgctggatg cccgttttcc
    12541 ctattcaatt ggatatggtc tgacagaaac ggcaccgctt attgccggag cagtccatcc
    12601 caatacttgc ctccaatcca taggtaaggt catggagggt atcagtatcc gtctgggcga
    12661 caaggatccc gataccggtg taggagagat ccaggttaaa acaccctgta tcatggaggg
    12721 gtattataaa aatcctgaaa ccgacaaaga ggcctttaca gacgatggct ggttccggac
    12781 aaaagacctt gccattatgg acaaccgggg acgtatcttt atcaaaggcc gcctgagcaa
    12841 tatgatagtg ggctcttcag gggaaaacat atacccggaa gagatcgaaa gcattatcaa
    12901 ccagcatgaa ttggtgactg aatccctggt ggtgcaaagg gacgggaaac tggtggctct
    12961 tgtttacatg caggacctga gccagaaact cagggaccag attactgagg tgcatgcaga
    13021 tatcatgaac tttgtcaaca gcaaggtggg caagatgtcc cgcattaccc gcatactgga
    13081 acaggagaac ggattcgaaa aaacaccgac tctcaagata aaaagatacc tttattcgga
    13141 gatgagaacc cgtaaagaca agaaacaggc tgtctgattt caggatgtga agctgacctg
    13201 aacctggaaa acatttttgt cgcccgtcct gccttcaata tagcgaccca ggggggctgt
    13261 ttccgccaca aaaagatcca gagtttctga tagcctggat tctgcattga aatttatacg
    13321 aaacatttcc acactatgtt ttgtgacaag ctccgtatcc aggcagtcca cggcccattc
    13381 gatatacctg gcgttgttgg tatgttggtt gaaatctata tcggagaaac aaaccttttt
    13441 actttctacg tagcacagtt catttttagg gacaggtatt ttctcacagg gggtggcaat
    13501 ggcatgccgg gtggcttttt cctgtagcaa tgaggcatgt aggatatgct gaggacgctg
    13561 gatacggcgg gtattgacat ggatgatcaa ccaggagctt gttgcccgga ccaggtcctc
    13621 tgtttcatcc cggttacgga taagaaaatc cctgagccag aaaatgccgt cttccccttt
    13681 gtgccaggtc tccatgacaa caggctcttt ccatacagga ggccgcacga acctgacctc
    13741 cacacgggac aacacccaga tagtctgctt ttctatgagc tgttcatatc caaatcccaa
    13801 tccaagggca tcctgattgg ccatttcctg ggccaggttc atgaaagcat aaggtttcag
    13861 gttcctggcc gcatctgttt cgtaacaggc aatagttctg gattctttga tcatatgcaa
    13921 atataagaaa aacgggcagc aataacccgt gtcatttgct gcccgttgca tggcctgtaa
    13981 gttttattgt tcctgtgccc tgcgtttcaa ttgcctggtt atacgtgcta taacctgttc
    14041 aataccggac tcgggttcaa taacgttgta atcatcccaa aatccctcgt cctgaaaatc
    14101 ggttacttta tccagggtga tatcggtaga gcgcacccgt tgctcggagg gaatctttat
    14161 ggatttgtca ctcctttcgg taatagccag ttcagatgtt atggtatacg tgttcttgaa
    14221 aagtttcctg tcccattttg aatcaaactt gatctcggta cgggaatagt caaaagtcca
    14281 gtcaccagca atttccttat attgaaccat ataggctgca taaagcactt cggccttgaa
    14341 acccaagggc ctgcgcgata tgaagatatt ggtggcatcg gaacggcctt caacgttcat
    14401 attgaattca atacggctta tggcctctga cttcatgtct atgtatatct tcccgcggaa
    14461 ccagatatca tccacattgt ctttttgttg aaatgctaca acgtggttta tctttccgtc
    14521 tatctgaacg ggctcttcca tggtgaaatc atatatctgg ttgatttcag gaacatttgt
    14581 tcccaggaaa ggatatttgg ccacgtcaat ttgtaatgcc gagttgatac ctccgcggaa
    14641 cttgacgaag accgtatcaa tacgggccca gtccacactg ccccttcctt tgaaaacccc
    14701 tacctggtct atgccgtaca tcctggaata ggaagatttg ttgatatcca gcacagcttc
    14761 tgtgagggta acgtaattac tgcctttgcg gatcatttcc ctgtagaaac cagtcatctg
    14821 catggtctcc tgtgggtagt tgtcgggaat tttttcaatg gcccgggcaa caagcccggc
    14881 agcattgatc ggggttacca ggattgcggg tagtttggtg gccacgaaat tcaggggaac
    14941 caccagttta tcacccttgt ttatcaaatc cttcagggca agtgtctgcg ggtggtaacc
    15001 aaggaaggag atccgaagag aatcgttggc aaaagctggt ggaaatttca gggaaaagta
    15061 tccttccccg ttggttacat tgcttatacc cctgccgact atgtaaagcg acgcaaaaac
    15121 cagcggttta cgggtttggg catctataac ggttccggac acactcaggt actcaggtgc
    15181 tttgtatgtt aatacaatgt attggttctc aaatacgtaa ttcagattgc tgttgccaaa
    15241 agcctctgaa agaaatgcag cgactgtctt tttcccttcg cggataggaa gaacagagtt
    15301 cacgttcaca tcctgtttgc tgtatacaaa caagtactcg gtttgttttt ccacctggct
    15361 gatgaacgat tccagtgtta tgcggtttcc cgatatggtt atgacggcat cctgcggaac
    15421 cggaatatcg ggttctgcgg ccgaaagcaa aaaagcactt atggaacaca gaatgattga
    15481 taaaaagatt tttttcataa ctttgtaaaa ttagaagtta atacatttat tgtgttagcg
    15541 gttcagccgg tggatgttgc agcgtctgcc ggcttttttt attcaatttt tatagtattg
    15601 gtttccctgt cttcatctat tttgaaacgg aaaatctttt tcatggtttt gaggatgctc
    15661 tccagcccgt catcctgcct gaattttgcg ttgaaacgat actgtgccag cgtctcattg
    15721 ttgacaatga tgttcgtatt gtaataaagt tccagcttgt tcactatttc aatgaatggc
    15781 acatcattga atacatatat gccgtctctc cagagaagga attcgctgtc ttcaaaaaca
    15841 gaacgctgcc agccgtcctg ttcccagacg atactttcgt agggacggat acggacaggc
    15901 ggtgcttctt gttcaggatt gactacttcc agggaacctt ccaccaggga tgtcttgaca
    15961 ttgtggtcct tgtaagcaaa aacgttgaat tttgttccca gtacacggat atcgtattgg
    16021 cccgtggtga ctatgaaggg catttcctga ttttctgcca cttcaaaaaa agcctcgcct
    16081 gtgagttcaa cacggcgctc attacctgta aacttgtttg gatatctcaa taccgattgt
    16141 gcattgagcc atacgacagt tccgtcgctc agacgaaagg atttggtcct gtttccgggc
    16201 ggagtgacct cgatcatata ggccgatggt tcttcgggca gtgacctggt gagtggctgt
    16261 agcagccccc acagggcgaa acccactaca agcatggcag cggcatatac agcgacctgt
    16321 ctgtagatgg agcggtgatt aattcttttc aatgctgcca gcttgcgggt agctgtttcg
    16381 gcatccgaag aggcagggaa aagggaagag acagccttca gattctgtat ccggcagaat
    16441 tcttttctga gctcaggatc tttctccaca acttcaagca gtgccctctt ttccagggga
    16501 gtgagctcat cggcaaaata tttgtgaagc atttcttcca tgattgattc tccttttctg
    16561 actatagaac gtttaacttg ataaatacca ccaaacggat ttgaaaaaaa tcagataaga
    16621 tgttccagat ctttcctcag ttttctgagc gcgattccca tttggttttc aacagtattg
    16681 acagagattc ccagaatttg tgagatcttg ctgtattttt taccctctac ccggctcagt
    16741 atgaaaattt cccggcattt cgggggaaga ttgtttatgg cttccatgac ggtctcctca
    16801 atatcttcgc cggggatcag accggtatcg agttgttcca gggctgcaag attcaacgcc
    16861 tgttcggccc tgtaatcctt gtctactttc ttatgtcgca ggtaatcgat gcatttgttc
    16921 tttaccaatg tataaagata ggcagacagg ttggtacgga tctgtatgat gcggtgtttt
    16981 tcccatagga acatgaaaac attctggaca atgttttcag cagcctcgtc cgagaggaca
    17041 tacgtgcgcg caaaacgaca caggcgggaa aaataggcaa tgtagatccc gtcaaaacgc
    17101 tctcgcgtaa tgctgatctc cacctcttta cccgggattg cccaattcat attgagcgat
    17161 tttcagggct ctttccgcat gacggccttc ctggaacttt gcttcaagcc attcctttac
    17221 aatataccgg gcatattccg gggctacgat tctggatccg agagccaata tattggcgtt
    17281 gttgtgttca cgggacagtc gcgcagaaaa gggatcgctg cacgcagcag cccggatacc
    17341 cttgaccttg ttggcgctta tggagatccc tatcccggtg ccacacacga caatgccttt
    17401 atcgcattca ccacgtgcaa cggcttctcc cacagcaatg ccaaaatcgg gataatccac
    17461 cctgtccggg ctcccggtac caaaatccct gaccttgtag ccaagtccgg ccaggtattc
    17521 cttgatattc tctttcaggt ggaaaccagc gtggtcgctt ccaagagcaa taacttccat
    17581 gtccattagt ttatcttaca aatttataat ataattcgta aattagcggc attatgaaaa
    17641 cttaccgtat tgaagccctg ataatagcaa tagctttagt tataatgggc ttgctgctta
    17701 aacatggcat agatggtttt tcaggaaggg acaggaccgt caacgtgaaa ggtttatccg
    17761 aaatggaagt gccggcagac aaggtgacct ggccgcttgt atacaaggag atcggcaaca
    17821 acctgcagga cctctatgtg aggatcaaca ggaacaacaa aacactggtt gactacctca
    17881 tatccaacgg gatatcagaa gaggaaatcg ggatcaatgc tccagagatc attgatatga
    17941 gcgcggaacg ctacagtacc acacccgcgc cttaccggta caacgttacc tcggtcatca
    18001 ctgtcagttc cacaaaggtt gacctggtta gaaaactgat agcagaccag ggcgaattgc
    18061 tcaagcaggg tattgccatc acagggggtg aataccgcta caatacagtg tactccttta
    18121 ccgggctgaa cgatatcaag ccacagatga ttgaggaggc aacgcgcaac gcccgcgctg
    18181 ctgccatgaa attcgcccag gactcgggaa gtaaactcgg caagatcaag aatgcatccc
    18241 aaggtcagtt taccataagc gacagggatg aaaatacgcc gtacatcaag aatgtgcgtg
    18301 tggtgaccac cgtgaattat tatttgaaag attaatacta tgaaccaaag gaaaattgta
    18361 gaaacgaccc tggccccgcc tgctataggc ccttattcac aagctgtcat tgcccagggc
    18421 tttctttata tgtcaggcca gctgggcata gatcctgcca caggagccat ggttgaatcg
    18481 ggcagggaag aacaattcga gcaaatcttc aagaatgcgg gtcatatact ggaagcggca
    18541 ggatgcggat tcaaggacgt ggtgaagtgt accggttttt tgaccgacat ggatgatttt
    18601 cccttgttca acaaggttta ttccaggtat ttccagttac catatcctgc acggtcggtt
    18661 tttgaggtat cggcccttcc caagaaaggt gccttcgcag agttggaatt agttgccaaa
    18721 atgactgata atctgtcatt ttgaccgttc caatcacgtt ttttccaaga ggtacggtat
    18781 ttgaataatt gattgttgaa attacaattg aagtttaaca attaattact caaggaggat
    18841 acagctatgt taccagcatt aagacacaca cagaactggt ggccctcatt gttcaacgag
    18901 ttttttgaca atgaaggatt ggtgaggttt gtaggaacga cttctcctgc catcaacgta
    18961 attgaaaacg aaaaggatta ccaggtcgag gtagccgctc ccggcatgtc caaagaggat
    19021 ttctgcatcc gtctggacca ggaaaatcgt ttgaccatat gcctggaaaa gaagaatgat
    19081 gattcccaaa aggataagaa cggcaaatac ctcaggcgtg aattttctta ctcgcactac
    19141 gaacaggcat tcctgttgcc cgatgacatt atcaaggaca agatagcagc ttccatggaa
    19201 aacggggtgt tgagaatatc catcccaaaa gacctggaga cgcctgcagt gcccaaagaa
    19261 tacacgatcg agatcaggta attcaacggt caggtagctg acagtaaaga acaggggagc
    19321 tttggtacgc cagggctccc cttgcttttg ctgcctgtta tcgctgacac cacaaggaac
    19381 gaagagcttt ttggggtacg ccgaggctct ccgttccttg tggtgtcagc gactgaggcg
    19441 ggcgcctctc atacattgct ctttaaactg ttattcaatg tgttatgtaa tgcgtacccc
    19501 cggtggtaag aaggaggttc cacctccttt gatcttttgg gattgtggtt gacagggagg
    19561 tggtaagtag cgataattgc gttcatgaca ataaaacatt taataacagc atgttacaag
    19621 acttcatgac aaaaccgcta cttaccacct ccccccaaaa taacgaaaaa tcgcaaaaac
    19681 cgggggaggt agtaagttgc gaaaagtgcg aatatgcctc tattttacac attaacggac
    19741 tgttatgcgt ttttgtaaca tagttgttac ttaccacctc ccgcaaaata tactgaaaaa
    19801 ttagtggtgc tgcgaagcag ccttttatca gcgccaaaag tctttggcgc accaccttga
    19861 agatcaccgt gcggttgttt gagcaggaat actattcaat ggcagggtcg ctacgttata
    19921 aagaacggag agcctcggct taaggaaaaa agctctccgt tctttatggc gtagcgacta
    19981 aacagagcaa aagcctcggc ttaaagaaaa agcttcccgg gtttgtggtg cagcgcctta
    20041 tagggcacgc ctatttgata agcatgtaga gcatcatgga aatgtaatag gcaatcccgc
    20101 tgtcaaaatg attatcgtcg gccctgatca ggttcacgtt gcctcccttt tccaggaagt
    20161 tgttgtacat cttctctgcg atttcaaagg gagccatatt atcattcacc gtgtggatta
    20221 tggtcatggg ggccctggga gcccattttt cgatcaccga gtggagcctg agacaggaat
    20281 ccaccttgtt gaacgaagca tttttctccg gggtgaacat gtcgggatgc atgtatgttg
    20341 taatgtcggg agataagaat tgagccattt caacggccag gtatttccgc gacagccagt
    20401 catccatgtt tgaaagcaac gggtctatga aaacctcgct gtagtcgatg ttcagtccat
    20461 accaatagtt catggcgaca tacatgtatg ggatgaacag gaatgtactg tagcgtgtct
    20521 tggtaagaat atccacggcc atgccaaggt catatacacc ccctcctgga taggacttct
    20581 ctactacaaa tccatctgta ccgtattgct cgatgtgccg gtgcagggct acaacaccca
    20641 tgccccccat ggaataacct cctatggtta ctttcctggg aaagggaagc ccaatggatt
    20701 gaaagtactc ggctgcagcc aggtgcatgt cgtaagccac ctgtcccgta ttgtcggcaa
    20761 agagaaaagg atattccctg tcgctgctta caccgtatcc tatcatgtcc ggtactatca
    20821 cagtataacc gaaaaagctc aggatgcctt caaaggttat catcaactca ctgccggtcg
    20881 tggttttgtc aattttagcc gagggaaaga aatggagcag gcccctggac tctctgtcca
    20941 ggggcctgag aacgatcccc gatgccagga tttcattgcc gttaggatct ttggtcaggt
    21001 aggatatggc ggtactatgt acttttcccg ttgtatccag caggctcatg agttgttcga
    21061 gttgtccgcc gtactgattg ctgaagttgg gataaatgct caccagggaa gagagcaggt
    21121 ctttgaaagc aaaggtgtct tgcgccaaca cttccctgaa atgcaccggt tcccgttcag
    21181 gcatgtccat gttcctttca caggaagcca gtacagggaa aatggaagat gcaagtgcaa
    21241 aaacgactat cttagaataa ttgcgaatac ctttttgact cattattcgc cgaaagtaca
    21301 aaaaaaaact aaaacagctt tttcaacaga gcagggattt ccaccggggt attttttatt
    21361 tgccagagct ttttctgtcc ttccggggtg agctcaaagt acatctttct ccggtcctcc
    21421 gagcacaggg aacgtataat ccatttcctg gattctgccg atctgagcac tttggaagtg
    21481 tgtgaggcag aaaagcctgt tttttcagca atagccgagg cagaaagacg tccgggttcc
    21541 ttttcgcatt ctccgagcaa acagagcacc atggcttcgt tgagcgagag agaatgagcc
    21601 tggatcaggc ttttttcaaa ttcattgaaa gccatcagga cgtctttcat catgcatatt
    21661 gaatccttca tgccccggat cagataaaca ggttaacatt tgattgtacg gccctgttca
    21721 tataggtggc aactcctcct atgatcacat tgtccatcag ttcttccgct ttcactccca
    21781 tcacatccat ggacatctgg caggcaatga attccacccc gttttcaatg gcctggcggc
    21841 gaaggtcctc cagggaatcg atacccctga ttttcatgat atgacgcatc atgatccgtc
    21901 ccatgccgaa catgttcatc ttggacaatt tcagtgcgcc gctgtgcgaa ggcaacatca
    21961 tcccgaacat tttcccgaaa atatctttct tgacggcagg tttcttgatt ttcttgatga
    22021 cattaagtcc ccagaaagta aagaatatgc tgactttttc cccggtggcg gctgctccgt
    22081 ttgccagcac aaaggttgcc agagctttgt ccaggtcatc actgaacatg atcagggttt
    22141 tccctttgcc tccttcaggg gctgcaggag cttgaatccc acgctctttc ttttctacaa
    22201 cagcagtgaa cactcctttg ctgtctgact gttgaaccag cacattccct gtagtctgac
    22261 accatgcctg tatatcacgt gcaaaacccg ggtcggtagc ctggatttcc accctctgcc
    22321 caggaactat ggaatcaatg ctgctcttta ttttcatgat gggaccggga cattgcagtc
    22381 ctgttgcatc aattttcagg gtattcacct caatggtggc tgcttttgaa gaggcagcgg
    22441 ctttgttctt tgccagtgga tcccccgagt ctgaataagg tgcagagaaa gccggagtga
    22501 tgggaatgga tttcttttcc gattcagctg cggcttcgta agtcttatat cctccactaa
    22561 ggttataaac gttattgaat ccattctgta tcaatatatt ggatgcaaga taccctctca
    22621 gtcccacagc acagaataca tatacaggtt tgtcacgggg aacctcgtcc agcctgttcc
    22681 tgagttcatc tacgggaata tttacggatc cggaaatggt tcccagacta ttttcagcag
    22741 ccgtacgcac atcgagtttg gttacttctc cgggtccggt tttttgcatt tttctccagt
    22801 aaaccggttt cattttgccg cttaaaatat tggatgccac atagccggca atggcaacag
    22861 gatcttttgc cgatgagaaa ggcggggcat aggcgtgttc catggtaatc agatcgtata
    22921 tgcttccttg ttttttaact acatgtgcca acagatcaat acgcttgtct actccgtcat
    22981 agcctactat ctgccctccg tacagtcgtc ccgtttccgg atcgaaagta attttggtac
    23041 tcatctgcag ggcattggga tagtatcctg catgagaatt agaatgtgta gtggaagtaa
    23101 gatagggaat attgagctgt ttgagttttt tgcccggaag cccggtagaa gccacagtca
    23161 ggtcaaatac cctggctatt gaagtgccga tggaaccttc gtactttata cggtccccgg
    23221 ataccatatt gtctgctaca atcctggcct gtctgttggc cggtcccgcc aggtaattga
    23281 gccagggttc gccgcttaca gggtggggaa attctatggc gtccccaatg gcataaacat
    23341 tttctgccga ggtccgcagg tattcgttca cttgtatacc tccagtacgg cccagcgtga
    23401 ttccggcctg ttttgcaagg gtcgtatctg gtctgacccc gatggaaaga atgacaatat
    23461 cggcctcaag ttcgttcccg tttttaagat tgatcttaat ggaaggatcc ggcccggaaa
    23521 cagggatgaa tccttgcact gcctggttca aatgcagatc tacatgtttc tggtaaagat
    23581 gctggtgcac caggccggcc atggaaaaat ctataggagt cataacctgt tcggccattt
    23641 ccacaatggc cacatgagcg cctgttgcat gcaggttttc tgccatttcc agaccaatga
    23701 aaccagctcc tacgaccacg gccctgcgga tggggtggga acgcatatat tccttgatac
    23761 gatccgtgtc atccacgttc ctgagggtaa agataccttc cgaatctatg cccggcagcg
    23821 gaggtttcac gggattggcc ccgggtgaaa gaagaagttt gtcataactt tccctgtatt
    23881 cgtctccttt tgcattcctg accgtaacaa gttgtttttc agtatcgatg cctatcactt
    23941 ccgagttcac cctgacgtcc acattgaaac gtttcccaaa actttctggt gtctgaacaa
    24001 aaagtttagc ccggtctttg atcacatcac ctatgtaata aggcaaacca cagtttgcat
    24061 aggaaatgta cttccctttt tcaaacatga tgatttctgc attttcatca tttctcctga
    24121 tgcgggctgc agttgtagct cctccggcca caccgccaat gataacatgt ttcatacgta
    24181 aatattttgt aatttagaaa agaaattaat gattatcgta aatatttgac aaaggtaaat
    24241 atttcccatg gaaaataaaa ttctttaccc ctttttttgt atcttttttt caattttcat
    24301 atccggctgc agacaatctg cccccgtgaa ggcccatgga ccggttccca caccggaaca
    24361 attggagtgg caaaaaatgg agtattatat gtttgttcac ttcggtccca acacttttac
    24421 gggggtggaa tggggcaacg gggcagagga tccggccgtt ttcaatccca gggcactgga
    24481 ttgcaggcag tgggccagga ccgctaaaaa tgccggcatg cagggtatca tcatcaccgc
    24541 taagcaccac gacggctttt gcctctggcc cagccgctac agcgatcata ccgtccggaa
    24601 cagtccctgg cgctcgggcc agggcgatgt gctggcggaa ttatccgctg cttgcaggga
    24661 atacggcctg aaattcgggg tttacctgtc tccctgggac aggaacaatc ccgcctacgg
    24721 cacgccggaa tacaaccggt tgtttgcaaa tacactcacg gaagtgctca cttcatacgg
    24781 acccgtattc gagcaatggt ttgacggagc ctgcggggac ctggtttctt caccctatga
    24841 ctggtccctg ttcaacagca cagtgcagga gcatcagccc ggagcgttga tattcagcga
    24901 tgtggggcca ggttgccgat gggtgggcaa tgaaaggggc ttcgccggtg aaacaaattg
    24961 gtccaccctg gacacttcag gtttcacacc gggaagggga gcgccgtcca ccatcattct
    25021 caactccgga cagcggggag ccccgtactg gatacccgtc gaggccgatg tctccctccg
    25081 gccaggatgg ttttacaacc ccgataccga taattccctc aagagcgttg aggaactgat
    25141 ggacatctat ttcggctcgg tgggcaggaa tgccaacctc ttactgaatg ttccccctga
    25201 cacccggggt cgcattcctg ccggggattc cctccgtctt atggaattca ggcagagacg
    25261 ggacgaagtc ttttccaggg atcttgcgcg gggtgacgct gctttcgcca cttctgtcag
    25321 gaatgatggt tcaaggaaat tccgtccgga taacgttaca gacgctgact atgataccta
    25381 ctggatcccg gaggagggaa taaccaccgc agcactgacc attgatttgg ggaaggaaga
    25441 aaatttctcg gcactcatgt tacaggaata tataccgctg ggccagcgga tcgcacgttt
    25501 tatcgtcgaa tatgccgatc cggaggaaga ccggtggctg ttgctggctc agggcaccac
    25561 catagggtac aagcgcctgt tccggttccc ggccgtcacc tcggacaaga tcaggatcag
    25621 catcctggaa gccgatgccc caccgctgct gaatgcgatt gccatctact gaaaaaatcg
    25681 tatttttgat acaaaatgaa caaactggcg aaatatatca ttgccgttgc tgcactggcc
    25741 atcatagttt tcatagtttg gtatttccgc agcatcatca cctatatcct gatagcggca
    25801 ctgctctccc ttgtaggaag cccgcttgta agattattgt cccgcataag gataaagaaa
    25861 tggcacattc ccaagtcggt atcttccatt ttggtcattt gtgccatgtt tggactgata
    25921 ttcctcttct tttactatat ggcacccctg gtcataggca tagcttcacg tattgatacc
    25981 attgggctgg aggatgtgcc ggccatgcta tccggctcat tggcacaggc aaacaaattg
    26041 atccatggcc tttttccttc acttgcccct tctatgacta ttgaatccct gatctggaat
    26101 gagatagaaa aattcttgca tcctgaattc tttaccaggc tatttgactc gctggcttca
    26161 ttttttgtga attttggcgt gggtttattc tctgtggtct tcattacctt tttctttctc
    26221 cgggaagaga acatgttttc aaatatgctg atggccatag tgcccgttaa atacgaagag
    26281 aggttctcca acgccatgaa atcggtgaat gaactccttc tcaggtattt catagggatt
    26341 ttcatagaag tccttatcat gaccattctc atttctgcag gactgcattt cgtggtacgt
    26401 atagaataca agctggcgct tgtcctggga cttatctttg gcatcctcaa tatcgtaccg
    26461 tatgccggtc cgctcattgc aggcagtatt ggaacggtta tcggtatttt aacttcttat
    26521 gcaacatccg gttattcaag tatcttgtgg tttgtggttg ccatggttgc cgtctatgcc
    26581 ggtatcaaca tgctggatac atatcttatt cagccggtga tctattccag cagtatcaag
    26641 gcacaccccc tggagatctt cctggtaatc ctcatttcag gatacatggg aggcgctgtg
    26701 ggaatgctgg tggccattcc tgcctacacc gtgatcaggg tatttgccag cgagtttcta
    26761 tcgcgcttta aagttgtcca gaaattgaca gccaaacttc cggatcatgg agattgagcc
    26821 ttctcagctg ctgattgcat tcgttctgac tctttttgcc ggcctgtcca cagggatcgg
    26881 atcactggcc gctttactgg ctaaaaggac aaataccagc tttctttccg tcaccttggg
    26941 attttcggca ggggtaatgg tatacatatc ctttatggag ctaatacccg gggccaggca
    27001 aatattgaca ccgctgcaag tggtgttgtc tttctttgga gggattgccc tgatcgccat
    27061 cattgacttt ctgataccca aggatgaaaa tccacacgag ttgcatctga cagaggatat
    27121 ggacaagaag agccgcctga agagaatggg cataatgata gcactgggca ttgcaatcca
    27181 taattttccg gaaggtatag cggctttcat agcaggtatg aaaagccttg atgtaggcat
    27241 acccattgcg ttggccatag ccatacataa catacccgaa gggattgccg tgtcggtgcc
    27301 catcttctat gctacaggaa accggaaaaa ggccttctgg tactctttac tgtctggcct
    27361 ggctgaacct tttggagccc tggtggccat acttatcttg tttcaatact gggacccttt
    27421 gcttgagggg atagtcatga cagccgtttc cgggatcatg gtatacattt ccactgatga
    27481 actgctgccg ggagcagaac gcttcggcaa gcaccatctg tcgatcctcg gttttatcgg
    27541 tggcatggcc atcatggcgt caagcttgct gctgatctga tttttagtag ctttgcaatt
    27601 atgagtaacg ttgttctgaa cgaaattcct cccttgtccg ataaggattg ttttcacgtg
    27661 gtggaacgtt ataaatctga gttcactttt ccactccacc agcacaggga atacgagatc
    27721 aatttcattg aacacggcaa gggtctgaaa aggatagtag gtgataacat tgaatacaca
    27781 gatgattacg acctgatact tatttcggga gacagcctgg aacatgtatg ggaccagggt
    27841 aattgcaaat caccggtgat ccgtgagatc accatccagt tttctcccga tctgctgcca
    27901 acctgtttgc tggaaaagaa ccagttcaat tccatccgca aaatgctgat caaggccgag
    27961 catgggctcg ccttccccct ggaaaccatc atgaaggtct ataatcttat tgataccatt
    28021 gccagggaag gaaaaggatt tgagcaattc ctgaggttgc tcaaactgct gaacgagctt
    28081 tccctgtgcg ttaacagcag gagcctggcc agcagttctt ttgcgcatgc cgagagaaat
    28141 atggaaagcc ggcgggtcag caaggtgaaa gattacatca accagaacta cacaaagaaa
    28201 ctcagcctgg atcttctttc ggaaatggtg ggaatgtcgc cggtcgcatt cagccgtttt
    28261 ttcaaattac gaaccgggaa gacgctttcg gattatatca ttgatatccg cctgggatac
    28321 gcctcacgta tgctggttga ttccaatagg acgatttctg agatctgttt tgaatgtggt
    28381 ttcaacaacc tttccaactt caacaggatt ttcaagaaaa agaagcatat gacccccaag
    28441 ctgttcaggg cccattaccg gaagagcaaa tcaacgatat aaaggataat gcaggcaagg
    28501 tcagcataaa tgcttctcct gttgtggtta tcttttctat ctctttcatt ccgtcatttt
    28561 catttgtaac ataaactaca gccttaccgg ccgggattcc ttcaagggtt gcctccctta
    28621 cagccccgtt gttcactatg tgtatagcat agtccccggt gccgggattt ccaaaggcgg
    28681 cacagttgat cagatccctg tcactttgaa cgggcataga aaaaacgttt tcaggtgtgg
    28741 aagccagctg cttcaagttc cagaaacgtt gtgtggggcg taaaagcccg gaagaaccat
    28801 atattccgtc tccccataga atggaataat cagaagtgag ttgccattgt agtatggata
    28861 atggctggca tatggcacaa atccttatat agagattgat ttcatagaat gcgaatgtgg
    28921 actccgcaaa tattgccggg tatcggtggg cagctgcatc ggtactgcct tcacctatga
    28981 tcagcggaac gtttagcctg cgtgctgcac cagcccattt ttcaagggtt gcctggtcgc
    29041 atcccctcca ggagtgaaat gataccgcag ctatatactt atgtgtagaa aggtcttcca
    29101 tagcaggcag aataaaatca aaggtggtgg catctgaatt gtctcccagt agaatttttg
    29161 ttgccaatcc ccgtgatgca agaacctgcc ccagttcccg tatgaagacg gcatgttccc
    29221 ggggggtatg gaacacgtca atccctatgt ccgattcgtt gaatgaaaaa gcataggctt
    29281 ctacgccata ccactttttc aggtaatcca ggtaatcggc tagtgaattg taaatggctt
    29341 cctttttttc aggatccagg cgataggctc tcacgcctcc tccccggcgc cagtattcac
    29401 taatgtctcc ctgaacagcc catgcgggag ggaaccaggc gctgacgatc actggcatgc
    29461 cccgggctga cagacgtcgt gccatttcca tagcatcctg cacacgctgg tcaggagcag
    29521 gggccttgtc ttcgaacggt tgccagttgg cccacggcat ttcaacccgg ccccatgcca
    29581 cacgcatgtt atccagacag tattgtatca cttttggatc catttgcgga ttctgtaacc
    29641 tgaaattgcc tccgaatccg tcaaaacgcc tgcccggaga ggtgaaatcg gctcgtattg
    29701 ttaccggttc cttgtctatg aggcccctgg cactaaggtt gaattccagg gtggctgatg
    29761 ttcctttttt tagctcagag agcaggagta tgaagataag atcccctttg cttaccaacg
    29821 ttggtctacg ggtaaagga
//
LOCUS       DAOSOF010000005        27729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1725_length_27729_cov_4.939257, whole genome shotgun sequence.
ACCESSION   DAOSOF010000005 DAOSOF010000000
VERSION     DAOSOF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27729
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_27729_cov_4.939257"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3311
                     /gene="purL"
                     /locus_tag="PLM68_00600"
                     /gene_synonym="purI"
     CDS             <1..3311
                     /gene="purL"
                     /locus_tag="PLM68_00600"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOT53903.1"
                     /translation="NEEEIGYLQDLSRRLNRSLTDSEIFGFSQVNSEHCRHKIFNGTF
                     IIDGEKQPSTLFEMIKKTAAVNPNRIVSAYRDNCAFISGPSQHVFFIPGFPDRPGFFK
                     TVPYNMVISLKAETHNFPTTVEPFNGAATGSGGEIRDRMAGGKASFPLAGTAVYMTSL
                     PRLEGGAERPEGRPWMYHSPEDILTKASNGASDYGNKFGQPLICGSLLCFEHTENNRQ
                     YGYDKVIMQAGGVGFARMEDAMKEKPGKGDKIVLLGGDNYRIGMGGGAVSSVATGEYG
                     NAIELNAAQRSNPEMQKRVYNTIRSLAENTVNPIVSIHDHGAGGHLNCLAELVEHEGG
                     KIDMEQLPLGDPTLSAREIIGNESQERMGLVMHPQDLELLRKTAERERAPLYVIGEIT
                     GDGRFTFENQATGEKPIDLEIKDLFGSVPRTVMEDQTCVISFEPLAYGQDNKSLMDYA
                     RQVIQLESVACKDWLTNKVDRSVTGWIAGQQCAGPLQLPLNDYGLTAIGFVPSPKGFS
                     GIATSLGHAPVAGLIDAAAGSRLSIAEALTNLVFVAPDNGLRDVSLSANWMWPCRNRG
                     EDARLYSAVKAASDFACQLGINIPTGKDSLSMTQKYPDGSKVLSPGTVIISAVAPVSD
                     VRRKVQPVMDCKTASAFIYVDFSGRTENACHLGGSALAQCLGKVGASCPDVEDAEAFK
                     RGFETVRELIAHDMVLAGHDISAGGLLTCLLEMCFANTTGGIRLREHALEEIAGADRA
                     AALFAEYPGVVLQVRADRIHQALGMLLEHDVKTRLLGTPCEERAIRVDETLSFDIEMF
                     RDLWFRTSYLMDIRQCGPVKARERFENYAAIPLQYEFPAWFDGTLEMPDRSGGSKPLP
                     KAAIIREKGSQCDREMAWMLYMAGFEVRDVHTTDLISGRETLDDIRLIVFVGGFSNAD
                     TLGSAKGWAGAFLYNPKAKETLDRYYARKDTLSLGVCNGCQLMAEMGLITPAAREKSP
                     KIKPNESGKFECNFVNVTIEGSNSVFLKSLQGSRLGIWVAHGEGRFSLPQEGDYAVAA
                     RYSYDKYPANPNGSPGAVAAVTSACGRHLAMMPHPERSIYPWNWAYYPAERASDAVSP
                     WLRMFIDARRFCMQ"
     gene            complement(3280..5256)
                     /locus_tag="PLM68_00605"
     CDS             complement(3280..5256)
                     /locus_tag="PLM68_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT53904.1"
                     /translation="MRIWILSILATCCLMCGCHADKGTCFALIADTHINSNSSTNSEG
                     LLTIIRDINSRDSIDFVIIAGDITEFGSDLEIELARGLLDSLEKPWYILSGNHDSKWS
                     ESGCNTFLETFGYEQFYFVHNHIAYIGTNSGPNMRMAPALVPRESLIWLDSVLRTIPA
                     RMPVVYVNHYPLTEDMSNYRKVIDLLATRNIQLALCGHYHTNEAFTTLGIPAVKNRTA
                     LPGRTGGRGYNVVSIQDGQVTFRERIAREEPDSSYTLPAWHSVTFTKRPSRIADTLAA
                     VDKKAAAERLTAAEAKDPEQQEQQGQHQDQEPEQQPEPGIAGLNVEVVWSWQDESDIG
                     SAAMADTLAGIVVFANTKGVVKAVALEDGKALWSTPTQGKIFSTPAICKDRVFIGSTD
                     KNIYCLDLHSGQIIWTYPVGKSIVASPAVMDGIVYIGGSDGTFRALDAANGSLLWEYP
                     HINGFMESRPYADSSQVVAGSWGNELYSFHPRTGAIQWVWENRHRGRGYSPAATWPVK
                     TNGKIFFTTPERKCYAIDARTGKTVWEAQGGRESIGLSEDGQQVYVKTMFDSLYAFQT
                     GPQTAVSQWRVAGGFGYEIGPSPVTEQDGLVFVPSATGSLYAYHRQDGSKAWQVRLSD
                     GLVNYAQPTGHSRLVVSTMDGRVMLIAYKNGGHL"
     gene            complement(5265..8075)
                     /locus_tag="PLM68_00610"
     CDS             complement(5265..8075)
                     /locus_tag="PLM68_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOT53905.1"
                     /translation="MKSKNFFSGILPPDRIKTDYLHRLAWGTDASFYRKIPRLVLFPE
                     TEEEVSEILDICNKKHIAVTFRAAGTSLSGQSLSDSVLMVAATRWDKYQVHDGGETIS
                     LQPGIIGDRVNEILKPYGRKFSPDPASLSSAMVGGIMANNASGMNCGTHANSDRIVRS
                     MRLVLADGTILDTGNKASRDSFSCTHARFLTELMQIREKVMGKPALVKHIRYKYSIKN
                     VTGLNLLPLVEFEDPFEMITHLIVGSEGTLAFFSRVTVSTEPLWPVRASSMTYFTSIE
                     EACRAVQALKELPVIGTELLDRKALRSIEGRRGIPDYIKDFPDGTTALLIETAAHDKK
                     ELDNKILTITRGLERFEKLRPVNFTDKKEEYSVLWNMRSGIFPSIGGMRPPGSTCLIE
                     DVAFHLEDLPRAVAELQEILTRHEYHDSAIYGHALEGNFHFILNQSFDTPQEVARYQA
                     LMQDVITMVTDKYHGSLKAEHGTGRNMAPFVEKEWGKEAFSVMRQIKELFDPKNILNP
                     GVIFNEDPLCHIKDFKPLPVVHPVVDKCIECGFCEVHCLTWGKTLSSRQRIIVQREIR
                     RLKETGENPGLLKQLDKDYFYPGRATCAGDGLCATSCPMEINTGELTHYLREQAHPRG
                     SRDEVLGMLSAHHFQGLKNIMRIVLRLAHTAHIVLGTRLMSWICKVANKMGFPLWTPA
                     MPGAHRVKPHHQESEADTVVYFPSCINQSMGVARGAPDNTPLTDKMVNLLQKAGYRVV
                     FPDHMDRLCCGTIWESKGMPHIADEKTAELNGALWIASHAGKYPVLCDQSPCLYRMRE
                     KIKNLDLYEPVEFIEKFLVPRLDFHSTDEPVALHITCTTRKMHLDNEMRRLAERCSTQ
                     VLIPAEVGCCGFAGDKGFTRPEINAYALRKLRPQLEAAGIRTGYSNSRTCEIGLMTNG
                     GIPYMSLVYLVDSCTTPKKPVK"
     gene            complement(8072..8761)
                     /locus_tag="PLM68_00615"
     CDS             complement(8072..8761)
                     /locus_tag="PLM68_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOT53906.1"
                     /translation="MKKIVVLTGAGMSAQSGISTFRDSGGLWENHPVEEVASIEGWYR
                     NPGIMLDFYNERRAALQGVQPNKGHEILAGLEKYFDVGIVTQNVDNLHERAGSSKVLH
                     LHGELTKARSSGNPDLVMDIGYAPIALGDKAPDGTQLRPHVVWFGEAVPQMERAMQWT
                     RQADIMVVVGTSLAVYPAAGLIDFAHHEVPLFLVDPKPAPVRRKNLHVIAQKAVEGLE
                     KLEKILLDNYL"
     gene            complement(8758..9924)
                     /locus_tag="PLM68_00620"
     CDS             complement(8758..9924)
                     /locus_tag="PLM68_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53907.1"
                     /translation="MNSPFQYNRPLQAPDVLGRTAGSYWLASNLLKGQHSVVWDKPKT
                     GKTSFINKVLVQISKNEKAPLVCRISCFNIRSEHSLYAALANALFSCVANTLGDWESL
                     AATLLPLSKPGIPVPNHQEHEFFLVFPDDLSGEAYIELARFPQAFSTHLGKPIIMVLE
                     SFQTLVEFSGTPSRPLFQKMVELWKSMKTVTWLICGSGSTASSGYSVFRELFDERRPF
                     YRFAEHIPLEPIEEKEFTDYIIRSFAKAGRVISRDYAGMIYQKMEGHPYYVQYLADIC
                     FSNTKGYMNDDMYQSGLEELLDIFQQSFESICQELTTLQIYFLRAVCMGVEQFCSIEV
                     MDKYHLKSSGNVSRIRDALERKGIIETVRKKPRFIDPLFRFWFEERYCKTYWLT"
     gene            10090..10398
                     /gene="rplU"
                     /locus_tag="PLM68_00625"
     CDS             10090..10398
                     /gene="rplU"
                     /locus_tag="PLM68_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT53908.1"
                     /translation="MYAIVDIAGQQFKVQEGWKVFVNRLEVEEGESINFDKVLLKDHD
                     GKVQLGKPYLEGAAVNATVLKHLKGDKVLVFKKKRRKGHQKCNGHRQHLTQIRIDTIS
                     "
     gene            10417..10689
                     /gene="rpmA"
                     /locus_tag="PLM68_00630"
     CDS             10417..10689
                     /gene="rpmA"
                     /locus_tag="PLM68_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT53909.1"
                     /translation="MAHKKGVGSSKNGRESQSKRLGVKLFGGQVAKAGNILVRQRGTV
                     HNPGDNVGIGKDHTLYALTDGIVVFRKKANNKSYVSIQPLAETAQA"
     gene            complement(10752..11870)
                     /locus_tag="PLM68_00635"
     CDS             complement(10752..11870)
                     /locus_tag="PLM68_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOT53910.1"
                     /translation="MEFSHHIFSNHPECSVQVSSFTPPSGERTEHHMIFQFSRQHDNP
                     GADTGTDCIAVLHSCMQEWLSSDPYRDSERVFVRYFLKDASVQRSRIHTRELFPATCA
                     VSFIEQPPANKTEMAVWIYLRDKGSVPERYTHFWTTEKFCLAGNVTQQTRKLLMDYQQ
                     WLQAKGCGMKGQCIRTWFFIKDIDTNYAAFAQSRTQYFSEIGMDKDTHYIASTGIEGS
                     NGAPASYVTMDAYAVGGLEPDRITYLRGLTNLSPTNIYGVTFERGVALRLDDRTQVLI
                     SGTASIDKWGNVMHPDDIQAQTHRMWENVEVLLQEAGSSFEDVAQIIVYLRRQKDFEL
                     VRESFRSRFPSIPTVIVMGRVCRPAWLIEMECIAISHT"
     gene            complement(11884..13656)
                     /locus_tag="PLM68_00640"
     CDS             complement(11884..13656)
                     /locus_tag="PLM68_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT53911.1"
                     /translation="MNIRRIFPLVIATASLITTYAQRSPIATPAVFPAATPALSASAA
                     PSLKPLRILAFYNLGEDANHVAFAESAISFFSGLNGSRELKFEATADPSAMNKENLGD
                     ADVVMMLNYFPLDKKQRKAFEEYMDNGGRWIGFHKAALYREGEWPWFTRFLGGVVFAD
                     YNWPPLPTRIQVEDPLHPVCKGVPESFIAPANEWYRFSPGLRENPKVQVLASVAAESF
                     PLGISRIFESEDLPIAWANREYRMVYMNMGHGGNGLSDASQNLLFTNALQWLLAPEMP
                     VEEKVAVAYVTSWKEAVPDPAFITHINYAFGHVNETFDGVIVDNPDRLRQIVALKKQK
                     PSLKVLLSIGGWGSGRFSEMVAVDVLRDSFAMDCKRVVEEFGLDVIDIDWEYPTKQGP
                     GISYAPEDTRNFTLLMRDIRKHLPDGMLLTLATAASGKFIDFKSITGYVDFVNIMTYD
                     IGLPPFHHSGLYPSSMTGNLSCYESVLAHVRAGFPLERLVLGIPFYGKTGPDFPKGMG
                     GYGKLIQLEEYVHCWDDVAKAPYLKDCNGKIVCTYDNPLSIGYKSRFIKDYGMKGAMY
                     WEYEGDDENGSLRRAVYEGVFAPE"
     gene            13724..15442
                     /locus_tag="PLM68_00645"
     CDS             13724..15442
                     /locus_tag="PLM68_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOT53912.1"
                     /translation="MHTVLQILNLAGSLGLFLYGMTLMSESLQKIAGDRLRRILSSIT
                     SNPLKGVLTGVAITSLIQSSSATTVMVVSFVNAGLLTLTQAIGVIMGANIGTTVTAWI
                     VSLVGFKADIAILAIPLIAVGFVMFMSKKQKRKNTGEMLIGFALLFLGLTFLKDSMPD
                     LSSSPETLKFLQNFTGHGFWSVLIFVGIGTLLTMILQSSSATVALTLVMVNYGWIPFE
                     IAASMVLGENIGTTITANIAAAVGNVSAKRAALAHTFFNVIGVIWAVALFRPFLRLVG
                     AIITGLGGADPFIPIAQAADPVASGNAMIYSVSLLHTMFNLINTTLLIWFVPILVKIV
                     TFVIKSPKEEEKYRLKFIQAGLLSTAELSIDQAKQEIIHFGNIITRQYAIIRNALDVK
                     DNDEFDTLFNKISYYEEVTDRIEFEIAEYLNKIGDEGISAESGNRIQSMYKIIGEMES
                     MGDSGYNLGRILQRKRNNNVSFDERMIRNVLGMMDLVDKGYEIMLFNLQADYTKLNNI
                     DNALDAEKDINKYRDMIREGNLLSLGKNEYDYLSGVYYMDLIAELERVGDFIINVSES
                     IMEIKS"
     gene            15447..16715
                     /gene="serS"
                     /locus_tag="PLM68_00650"
     CDS             15447..16715
                     /gene="serS"
                     /locus_tag="PLM68_00650"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT53913.1"
                     /translation="MLTIKLLREDPDFVAERLAIRNIDARPVIQQIISLDTQRRAAQT
                     EADALTAEQKKMASRIGKLMAEGKLDQVAEIKKNAGESKEKVKALIADQEKYEKELNE
                     LMVTLPNLPHKMVARGEGAQDNVLVREGGQIPTMIQGALPHWELARKYDLIDFDLGVK
                     LTGAGFPVYKGKGARLQMGLIQFFLDQNAGEGYQEIMPPVIVNEDSGYGTGQLPDKGQ
                     QMYHVGLDNFYLVPTAEVPLTNIYRDTILEASKLPVRLTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQLTHPDHSYSALDDMVKHVERLVQMLELPYRILRLCGADLSFTSA
                     MTYDFEVYSAAQQRWLEVSSVSNFETFQSNRLKARFRGEDGKTQLVHTLNGSALALPR
                     IVAALLENNQTEDGIIIPEVLHKYLHFSRI"
     gene            16719..17276
                     /gene="ruvC"
                     /locus_tag="PLM68_00655"
     CDS             16719..17276
                     /gene="ruvC"
                     /locus_tag="PLM68_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT53914.1"
                     /translation="MKNGQIIIGIDPGTRLMGYGVIRINGSKMETLALGVVRMASIKD
                     HYRRMSHILEKMYELLDAFQPDCMAIEAPFYSKNVQSMLKLGRAQGVAIAAALSRNVP
                     VCEYAPRKIKMAVTGRGAASKEQVAAMLKHMMHFTQEFESLDATDALAVAVCHANQLK
                     LQEKTAPAGSWEAFVSKNPQRIVRK"
     gene            complement(17245..18273)
                     /locus_tag="PLM68_00660"
     CDS             complement(17245..18273)
                     /locus_tag="PLM68_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT53915.1"
                     /translation="MRDEQELFEHFHIVVDKGQSPLRIDRFLTSKLEAISRNRIQLAA
                     ESEYILVNGTPAKSNYKVKPLDDIRIMLPFEKREFRLIAEDIPLDIVYEDQDILVVNK
                     PAGLTVHPGHGNYTGTLLNGLAWHFGERGTVKTDDSRMGVLVHRIDKDTSGLLVVARS
                     EVAQFRLAKQFFDHSADRLYVALVWGDMESDQGTVTGDIARHPSDRLRFRVCPDGVNG
                     KHAVTHYRVLERFGYVTLVECRLETGRTHQIRVHMEHIGHPLFGDVRYGGDRIRKGTL
                     YSKYKQFIDNCFEILPRQALHAATLGFIHPATGEKVHFQAPLPDDMKQVLDKWRRYSI
                     SSQSFEDS"
     gene            complement(18274..19032)
                     /locus_tag="PLM68_00665"
     CDS             complement(18274..19032)
                     /locus_tag="PLM68_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT53916.1"
                     /translation="MFLVVLFLGIYTFLHLFTRHGKGLLVPDFSGLSMEEARKTARPL
                     HLRLEVTDSLYMPELPAGVIFRQVPPPGQHVKKNRRIELTVNSLLPRQVSVPSLVGFS
                     LRQAKAELASHGLKLGRLIYVPDLATNNVLEQMYQGQPLPAGAPINIYSAIDLKLGMA
                     PGHELAYIPMLTGKSLEAALDIISDHSLNIGSIKYDGTVLTAADTLAARVFSQSPDFS
                     HQPAYPLGTSIHLVLTINPDLLIPRDITITEEDL"
     gene            complement(19092..19244)
                     /gene="rpmH"
                     /locus_tag="PLM68_00670"
     CDS             complement(19092..19244)
                     /gene="rpmH"
                     /locus_tag="PLM68_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT53917.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMSTPNGRRVLAARRLRGRKKLTV
                     SDEDRF"
     gene            complement(19258..19944)
                     /gene="rsmG"
                     /locus_tag="PLM68_00675"
     CDS             complement(19258..19944)
                     /gene="rsmG"
                     /locus_tag="PLM68_00675"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT53918.1"
                     /translation="MDADVVFRFFPHLDQAARKRIMELAGLYAFWNERINVISRKDLE
                     HLYVRHVLHSLTLSAAIKDDPFQAGATVLDAGTGGGFPGIPLAILHPQSRFILCDSIA
                     KKIKVVREISDSLGLDNVTAVVSRVEQMPDRCCDVMVSRAVTSLAGFIPWTRNKLREP
                     GRILFLKGGDLEAEIKEAAIKCRIPASCFSVTPLADTYRGLEDDFFETKKICEIKQTS
                     YLCAPLLKSS"
     gene            complement(19946..20500)
                     /locus_tag="PLM68_00680"
     CDS             complement(19946..20500)
                     /locus_tag="PLM68_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT53919.1"
                     /translation="MSQSLDQLDDRALVELALQNRQEAYTRLLVRHKDRVQAFINKLI
                     INSSESEDLVLMSFDKAFRNLKNYNPKYAFSTWLYSIVQNLCIDHFRKNKQFFVSLEE
                     AQDAEDVNPEDVLIAEQQKQIMENMISRLKPEYGEVIRLRYMHHYAYEEIANELNIPL
                     GTVKTRIHRAKVSLARIIELESKK"
     gene            complement(20505..21641)
                     /locus_tag="PLM68_00685"
     CDS             complement(20505..21641)
                     /locus_tag="PLM68_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT53920.1"
                     /translation="MDLTLLYQEPLLYILTLGSFVVITLFLFVQYARPAFGKRDRNPV
                     VKEEAPGVSVVLCVRNEYDNLVRLLPALLEQEYETFEIVVVDKNSEDDTEVLLASLEH
                     FHKNLTVRILSANAKFGRDELMALGVGIRAAKYPSVVFFRPDCFPSSKKWLVSLVKTA
                     LEKTADTVMGYTSFTGSSLLVRYDMMDQQLHQMGATRRKMPYVSWGNNILFQKDKFLN
                     GREFETSTTAFNQSEQAIISHVLEKNNTHSCVYPCGTVVLDRKITLQEYHHNRTERLC
                     TLMLTKKRAWFLLTLEKVFIAAFYILLAFACVRIGKTGDSLLMILPGGLLLLRWITVW
                     MHYIGFGLHLDEKRLAYAAPVWDLFSPLVHFYYLVASLIKRLKN"
     gene            complement(21651..24293)
                     /gene="polA"
                     /locus_tag="PLM68_00690"
     CDS             complement(21651..24293)
                     /gene="polA"
                     /locus_tag="PLM68_00690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT53921.1"
                     /translation="MKRIFMIDGHALLYRAYFAFLNRPMVNTKGVDTSAIYGFCRSLF
                     DIILKEKPSHLLVAFDPGGKNFRHEAFPEYKANRPETPQVIKDSLPVVREFLESCRIP
                     VLTIEKAEADDVIGTLSKRAENEGFKVFMVTPDKDYGQLVSENILMYRPLNHNDGWEI
                     MGVKEICEKYGISDPGQVIDILAIWGDTSDNIPGVRGIGEVGAKKLVSRYGSVENILE
                     NLGELSSSLQDKLNQSRETLFLSKDLATIRTGLDIPWNEESFRINAPDLVRLKELLRE
                     YEFHSLSRMLPRLEELFCCNGEQAPKAVSVSLSPPGSIIPADNPAVVETIIKEALRQG
                     KAGLAGSPQQLVLFAGNNAYLTTAALAAPLLGNRNIPKCGFKLKDLMLPLSLQSIPLE
                     GMLWDPDVMHYLVNPERNHRPADLAIQYLNYFPAEKPMESRSLFDAHPEDDPGIQNLV
                     NEAFIAFHLKEPLLEVLRNDAMETLYTELEMPLMEVLAAMEKEGVRVDIRQLEKYGSE
                     LNKSALEIERQIREHAGDPTLNVSSPKQVGIILYDKLKIDDRSRKGKKQYTTDEETLG
                     ALADRHPIVPLILEYRSLKKMLSSYIESIPPLVDPVTGRIHTTFNQTVTATGRLSSQK
                     PNLQNIPIREERGREIRKFFVPRDGDHILMSADYSQIELRIMAHMSGDEDFIAAFHAG
                     ADIHTATAAKIFHCSKSEVTPEQRSKAKTANFGIIYGISAFGLASRLRIPRKEASELI
                     EGYFEHYPKVKEYLDSTIASAKEKGYVSTLFNRRRYAPDIKAPNPMLRGLAERNAINA
                     PIQGTAADIIKKAMVAVHRALRDKGLKSRMILQVHDELLFDVYRPEKEIMEQLVRSHM
                     ENIAPLSVPLSVGIGFGENWEEAH"
     gene            complement(24290..24721)
                     /locus_tag="PLM68_00695"
     CDS             complement(24290..24721)
                     /locus_tag="PLM68_00695"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOT53922.1"
                     /translation="MSALHITREFRFEGAHALTHYDGKCRHIHGHSYRLMVTVTGEPS
                     LVPEDPKSGMIMDFNDLKQIVTRHVLEPLDHSLMLRKDAPLARELAESYDNVIIFDFQ
                     PTCEQLVRYIAGILEPVITPPNRLCRVRLYETPTSYAEWVK"
     gene            24879..26090
                     /locus_tag="PLM68_00700"
     CDS             24879..26090
                     /locus_tag="PLM68_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT53923.1"
                     /translation="MQQNYLDSIISDNAKNMRRSAIRDLLKMATRPEIISFGGGFPDI
                     NAFNVDELNEVIQEVMRENALQALQYGETLGVKRLREALAAHYRKQGMDVTYENFIIT
                     TSSQQAIDMVTQLFINPGDTLVCGLPSYLGALQAFYAHRANPVGIPKDEELRTVVNAL
                     VTAGKKPKFIYAIPDFQNPSGVTMTLKQRLNVLEVAKEFDILVLEDSPYREIRFSGEP
                     MPLIYSLDKEGRTMLLGTFSKTFIPGFRLGWVMAPPAIIEKLEIVKQSTDLCAPVFNQ
                     FIAARYMEKGYFDKNIERIKINYRNKRDNMMAAFAKYMPEGVTWTNPEGGLFLFVTLP
                     EGYDATELFRIALKKDVAFVIGEAFHCDGSGKNTLRINFSFTEESRAVEGVRRLADSI
                     RQMMEERKAKG"
     gene            complement(26080..26676)
                     /locus_tag="PLM68_00705"
     CDS             complement(26080..26676)
                     /locus_tag="PLM68_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT53924.1"
                     /translation="MMNMLDYIRKNRSCRRFVQDVKIGIPELESMIEAARLSPSAANR
                     QPLKYILCGEADSARGKEIFECLAWAAYLKDWPGPAEGERPAAYIIMLHDKTIHQQVL
                     CDPGIALQSILLQAVYLGYAGCIFASVNRPRLQQYLSLPEHLEILYVVALGKPAETIV
                     LEKINPGLPAGESIKYWRDEAEVHHVPKRSLEDLIINL"
     gene            complement(26687..27385)
                     /locus_tag="PLM68_00710"
     CDS             complement(26687..27385)
                     /locus_tag="PLM68_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT53925.1"
                     /translation="MSGILDILDVSVGYSWHKPLFEHINAQLDSGTVTGLLGPNGIGK
                     STLLKTIAGLLPPLKGSIRHGEGVKKNDFCAFVPSQPLRAVHFSVRDTVSTGRYRYTN
                     WLGMEDREGKLIVESVLERAGIKHLADRNSASLSDGEYHRVAIARALVQDTPLILLDE
                     PTAFLDIANKRSIGLLLRKLAQEEDKGILFSTHDLSLAMEHCDFFWIMTPERELHRGT
                     PAVMKEQGIIDRLY"
     gene            complement(27382..>27729)
                     /locus_tag="PLM68_00715"
     CDS             complement(27382..>27729)
                     /locus_tag="PLM68_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT53926.1"
                     /translation="MGEQYAQNLGISLSRVRNRIFLATTLLAGSVTAFCGPIGFIGIA
                     VPHMARMLFRNANHRVMIPASALTGACVMILADIISQLPGTQSVLPVNTVAALMGIPV
                     IIYVIFKNRAVDL"
BASE COUNT         6624 a         7055 c         7208 g         6842 t
ORIGIN      
        1 tgaatgaaga ggagataggt tatcttcagg atctgtcccg gcgcctgaac cgctctctga
       61 cagactcgga aatattcggt ttctcccagg tcaactccga gcattgccgg cataagatat
      121 tcaacggcac attcatcata gacggggaaa agcagccttc caccctgttt gaaatgatca
      181 aaaaaacagc ggctgtcaat ccaaaccgca ttgtgtcggc ctacagggac aattgtgcct
      241 ttatctctgg tccgtcgcaa cacgtgtttt tcataccagg atttccggac cgtcccggtt
      301 ttttcaagac agtcccctac aacatggtga tcagcctgaa ggccgagacc cataatttcc
      361 ccaccacggt agaacctttc aacggggcag cgaccggcag cgggggagag atccgggaca
      421 ggatggccgg cggaaaagcc tctttccccc tggccggcac ggccgtttac atgacatcac
      481 tgcccaggct tgaaggcgga gcagaaagac cggagggtcg cccctggatg taccattctc
      541 ccgaagacat cctgacaaaa gcctccaatg gggccagcga ttacgggaac aagttcgggc
      601 agcccctcat atgcggatcc ctcctgtgtt ttgaacacac ggaaaataac cggcaatacg
      661 ggtatgacaa ggtgatcatg caggccggcg gtgtgggttt tgcccggatg gaggatgcca
      721 tgaaggaaaa gcccggcaaa ggagacaaga tcgttctcct gggcggggac aattacagga
      781 taggcatggg tggcggggca gtgtcgtccg tggccaccgg tgaatacgga aatgcgatag
      841 aacttaacgc tgcgcagcgt tccaaccccg agatgcagaa acgtgtctat aacaccatac
      901 gttccctggc cgaaaacaca gtgaatccca ttgtctccat tcacgaccac ggggcaggag
      961 gccatttgaa ctgccttgcc gagctggtgg aacatgaagg cggcaaaatt gacatggagc
     1021 aattgcccct gggggatccc accctgagtg ccagggagat cataggcaat gaatcacagg
     1081 aacgcatggg cctggtgatg cacccgcagg acctggaatt gctccggaaa acagcagaac
     1141 gggaaagggc tcccctatat gtgataggag aaatcacagg agacggacgc ttcacgtttg
     1201 agaaccaggc aacaggagaa aaacccatag acctggaaat caaagacctg ttcggttccg
     1261 tgccgcgtac ggtcatggag gaccagacat gcgtgatatc atttgaaccg ctggcttacg
     1321 gacaggacaa caagtcattg atggattatg cccggcaggt gatacagctt gaatcggtcg
     1381 cctgcaaaga ctggcttacc aacaaggtgg accgctctgt cacgggatgg attgcggggc
     1441 agcagtgtgc cggaccattg cagctgcccc tgaatgacta cgggctgaca gccatcggct
     1501 ttgtgccttc ccccaaaggt ttttcgggca ttgccacttc gctgggacat gcacctgtgg
     1561 ccggacttat cgatgcggca gcaggcagcc gcttatccat tgccgaggcc ctgaccaacc
     1621 tggtatttgt tgccccggac aacggattgc gtgacgtttc cctgagcgcc aactggatgt
     1681 ggccttgccg gaacaggggg gaagacgccc gtctgtacag cgccgtgaag gccgccagtg
     1741 attttgcctg tcaactgggc ataaacatcc cgacagggaa ggattccctt tccatgaccc
     1801 agaagtaccc cgacggaagc aaagtgcttt ctcccggcac tgtcatcatt tctgcggtgg
     1861 ctccggtgag cgatgttcgc cgcaaggtgc agcccgtgat ggattgcaag accgcctcgg
     1921 cttttattta tgttgatttc tcgggaagga ccgaaaacgc atgccacctg ggtggctcgg
     1981 cacttgccca gtgcctggga aaagtgggcg cctcttgtcc cgatgtggaa gatgctgagg
     2041 cctttaaacg gggatttgaa acagtccggg aactaatagc gcatgacatg gtccttgcag
     2101 ggcacgatat ttcagccgga ggcctgctga cctgcctgct ggaaatgtgt ttcgccaata
     2161 ccacaggagg aatccgcctc agggaacatg cactggagga aatagccgga gcagacagag
     2221 ccgccgccct ttttgccgaa tatccaggag tggtcctgca ggttcgggcc gacaggatac
     2281 accaggctct ggggatgttg ctggagcatg acgtgaaaac caggctgctt ggcactccct
     2341 gtgaggaacg tgcaatcagg gtggatgaaa ccctctcgtt tgatattgaa atgttcaggg
     2401 atctgtggtt caggacctcc tacctgatgg atatccgcca gtgcggcccg gtgaaagccc
     2461 gcgaacggtt tgagaattac gccgcaatac ctttgcaata cgagtttccg gcatggtttg
     2521 acggcacact ggaaatgccc gaccgctcag ggggaagcaa gcctctgcca aaagcagcta
     2581 tcatcagaga gaagggatcg caatgtgaca gggaaatggc ctggatgtta tacatggccg
     2641 gatttgaagt acgcgatgtg cacaccacag acctgatcag cggacgcgaa acactggatg
     2701 acatacgcct gatagtcttt gtgggcgggt tttccaatgc agacaccctg ggttccgcca
     2761 agggatgggc aggtgcgttc ctgtacaatc ccaaggcaaa ggaaacactg gaccgttatt
     2821 atgcacggaa ggataccctc agcctgggtg tatgcaacgg atgccagctg atggctgaaa
     2881 tggggctgat cactcctgct gcccgggaaa agtcacccaa gatcaaaccc aatgaatccg
     2941 gtaaatttga gtgtaacttt gtcaatgtca ccattgaagg aagcaactca gtatttctga
     3001 aatccctgca gggaagcagg ctcgggattt gggtcgccca cggcgaggga aggttctcgt
     3061 tgccgcagga aggagattac gctgtggctg ccaggtattc ctacgacaag tatccggcca
     3121 atcccaacgg atcaccaggg gctgtggcag ctgtcaccag tgcttgtgga cgccatctgg
     3181 ccatgatgcc gcacccggaa cgttccattt acccgtggaa ctgggcttac tatcctgcgg
     3241 aaagggcttc ggatgctgtt tctccgtggc tcaggatgtt tatagatgcc cgccgttttt
     3301 gtatgcaata agcatcaccc tgccatccat ggtggaaacc accagcctgg aatgtcctgt
     3361 gggttgcgca taattgacaa gtccgtcgga aaggcggact tgccatgctt ttgagccgtc
     3421 ctgccggtgg taggcatata atgatccggt ggccgacggc acaaagacca gcccgtcctg
     3481 ctctgttaca ggggaaggtc ctatctcgta gccgaaacct cctgccaccc gccattggga
     3541 cacggctgtc tgcgggccgg tctggaaggc gtacagggaa tcgaacatgg tcttaacata
     3601 aacctgctgc ccgtcttctg agagccctat gctttcccgc cctccctggg cttcccatac
     3661 ggtttttccc gtacgggcat caatggcgta gcattttctt tcgggggtgg taaagaatat
     3721 ttttccgttt gttttcaccg gccaggtggc tgccggagag tacccacgtc cccggtgcct
     3781 gttttcccat acccactgga tcgctcctgt acggggatgg aacgagtaga gctcattgcc
     3841 ccaggacccg gcaaccacct gggaggagtc tgcatagggt cttgattcca taaaaccgtt
     3901 gatgtgcgga tactcccaga ggagcgaccc attggccgca tccaaggcac ggaaggtgcc
     3961 gtcagatccg ccaatgtaca cgatgccgtc cataaccgca ggggaggcca ctatggactt
     4021 tcccacgggg taggtccaga tgatctggcc actgtgcagg tccaggcagt aaatattttt
     4081 gtctgtcgat ccaatgaata cacgatcctt acaaatggca ggggtcgaga atatcttgcc
     4141 ttgtgtgggt gtgctccaga gcgccttccc gtcttccagg gctaccgcct tgacaactcc
     4201 tttggtgttc gcaaaaacaa cgatccccgc tagcgtatcg gccatggccg ccgacccaat
     4261 atcgctctca tcctgccagc tccagacaac ttccacgttt aggcctgcaa tacccggctc
     4321 aggttgctgc tcaggttcct gatcctggtg ctgcccttgt tgctcctgct gttccggatc
     4381 ctttgcttct gctgcggtta gtctctctgc agcggccttt ttatccactg ccgccagggt
     4441 gtctgcaatc cgggatggtc ttttggtgaa agtcaccgag tgccaggcag gcagggtgta
     4501 tgaactgtca ggttcctccc gggcaatacg ctcccggaat gtcacctgtc cgtcctggat
     4561 actaaccacg ttatatcccc ttcctccggt ccttcccggt aaagccgtcc ggtttttgac
     4621 agcaggaatg cccagcgtgg tgaaggcttc gttcgtatga tagtggccgc acaaggccag
     4681 ctggatgttc ctggttgcca gcaaatcaat gaccttgcgg taattgctca tgtcttcggt
     4741 caacgggtaa tggttcacat agacaacagg cattcgtgca gggatggtcc tgagcacact
     4801 gtcaagccag ataaggcttt cccttggtac aagtgcaggg gccatgcgca tgttgggccc
     4861 cgagttggtg ccaatgtatg caatatggtt gtggacaaaa taaaactgct cgtagccaaa
     4921 ggtctccagg aatgtgttgc atccgctttc agaccatttg gaatcatggt ttccggacag
     4981 tatgtaccat ggtttttcca gcgagtccag caatcccctg gccagttcaa tttccaggtc
     5041 ggacccaaat tcggtgatat ccccggcaat gatcacaaaa tcaatgctgt cccgggagtt
     5101 gatgtcccgg attatagtca atagtccttc actgttggtg gaagagttgc tgttgatgtg
     5161 cgtgtcggct atcaaggcaa aacaagtccc tttgtctgca tggcatccgc acatcaggca
     5221 gcaggttgcc agaatactta atatccagat tctcatttat ttagttattt aaccggtttt
     5281 tttggggtag tacaggaatc cacgagataa accagggaca tataaggaat gcccccgttg
     5341 gtcatcagtc cgatctcgca ggtacggctg ttggaataac cggtgcggat accggctgct
     5401 tccagttgcg gtcttaactt cctcagggca taggcgttga tctcgggccg ggtgaaacct
     5461 ttgtccccgg caaagccgca acatcccact tcagcaggaa tgagcacctg cgtggaacac
     5521 ctttcggcca ggcggcgcat ttcattgtca aggtgcatct tccgggtggt gcaggtgatg
     5581 tgcagcgcca ccggttcatc ggtggaatgg aaatccagcc tcggcacaag gaacttttca
     5641 atgaattcca ccggctcgta aaggtccagg tttttgatct tttcccgcat gcggtaaaga
     5701 caaggactct ggtcgcaaag cacggggtat ttccctgcat ggctggcaat ccaaagggcg
     5761 ccgttcaatt ccgccgtttt ttcatcagca atgtgcggca tgcccttgct ttcccatatg
     5821 gtgccgcagc aaagcctgtc catgtggtcc ggaaaaacaa cacggtatcc ggctttttgc
     5881 agcaggttca ccattttatc ggtcaggggt gtattgtccg gtgccccacg ggctactccc
     5941 atgctctgat tgatacagga agggaagtag acaactgtgt cggcttcgct ttcctggtgg
     6001 tggggcttca cccggtgggc accgggcatg gccggggtcc agagcgggaa tcccatttta
     6061 ttggccactt tgcatatcca gctcatcagg cgggttccca gaactatgtg ggcagtatgg
     6121 gcaagtcgca gtacaatcct catgatattt ttcagcccct ggaaatggtg ggcagagagc
     6181 atgccaagca cttcatcgcg agaacccctg ggatgtgcct gttcgcgcag gtaatgcgtc
     6241 aactctcccg tatttatctc catggggcag gaggttgcgc agagtccgtc ccccgcgcag
     6301 gtagcacgtc cgggatagaa ataatccttg tccagctgtt tcagcaatcc gggattctct
     6361 cctgtttctt tcagacgccg gatctcacgt tgtacaatga ttctttgcct ggaactgagg
     6421 gttttgcccc aagtcaggca atggacctca caaaagccgc attctatgca tttgtccact
     6481 acaggatgca ccaccggcag gggtttgaag tcttttatat ggcatagcgg gtcctcattg
     6541 aagatcactc cgggattcag gatattcttt ggatcgaaca gctctttgat ttgcctcatg
     6601 acagagaagg cttccttgcc ccattccttt tccacaaagg gcgccatgtt ccttcctgtt
     6661 ccgtgttctg ccttgagcga tccatggtac ttgtcggtga ccatggtgat aacatcctgc
     6721 atcaatgcct ggtatcgtgc cacttcctgc ggtgtatcaa aagactggtt caggatgaag
     6781 tggaagttgc cttccagggc atgaccgtat atggcactgt cgtggtattc atgcctggta
     6841 aggatttcct gcagttcggc aacagcccgg ggcaggtctt ccaggtggaa ggccacgtct
     6901 tctatcagac aggtggaccc gggcggcctc atgcctccta tggatgggaa gatgccggaa
     6961 cgcatgttcc ataatacaga atactcttcc tttttgtcgg taaagttcac gggacgtaat
     7021 ttttcaaaac gttccaggcc acgggttatg gtaaggatct tattgtcaag ttccttttta
     7081 tcatgggctg ccgtttctat caggagtgcc gtggtgccgt ccgggaaatc tttaatgtaa
     7141 tccgggatcc ccctgcgacc ttcaatagaa cggagggctt tcctgtccag aagctccgtg
     7201 cctatgacgg gaagttcttt cagggcctgg acggcccggc aggcttcttc tatggaagtg
     7261 aaatacgtca tggaactggc acgaaccggc cagaggggct cggtactgac agtaacccgg
     7321 gagaagaaag ccagcgtacc ttccgatccg acaatcaggt gtgttatcat ctcaaaggga
     7381 tcttcaaatt ccaccaaagg aagcaggttc aggcctgtca cgttcttgat gctgtatttg
     7441 taccggatat gcttcaccag ggccggtttg cccattacct tttcacggat ctgcatcaat
     7501 tcggtcagga acctcgcgtg cgtacagctg aaagaatcgc gggatgcctt atttcccgta
     7561 tccagtatcg taccgtctgc caggaccagg cgcatggagc ggacaatcct gtcgctgttg
     7621 gcatgggttc cgcaattcat cccggaggcg ttgttggcca tgatgccacc caccatggca
     7681 gacgaaaggg aagccggatc gggggagaat tttctgccat aaggcttgag gatctcattg
     7741 acccggtctc caatgatgcc gggctgtagc gatatggttt ccccgccatc gtgcacctgg
     7801 tatttgtccc agcgtgtggc agccaccatc aggacactgt cggaaaggct ctgacccgag
     7861 aggcttgtcc ctgctgcgcg gaaagtcact gcaatatgtt tcttgttgca aatgtccagg
     7921 atctcagata cttcttcctc ggtttccggg aacagtacca ggcggggtat tttacgatag
     7981 aaactggcat cggttcccca ggccagacga tgcaagtagt ctgttttaat acgatccggc
     8041 ggcagtatgc cggaaaagaa attcttggat ttcataaata attatcaagc aggatctttt
     8101 cgagtttttc aagaccttca acggcttttt gagctatgac gtgcaggttc tttctcctga
     8161 ccggggcggg tttcggatca accaggaaca aagggacttc atgatgcgca aaatcaatga
     8221 gtccggctgc aggatatacg gccagggaag tgcctacaac caccatgata tctgcctggc
     8281 gggtccattg catggcacgc tccatttggg ggacagcttc cccaaaccat accacatgcg
     8341 gacggagttg tgtcccgtcc ggggctttgt cacccaaggc tatgggagca taaccgatat
     8401 ccatgaccag atccgggttg ccggaacttc ttgcttttgt caattcgccg tgcaggtgaa
     8461 ggactttact gctgcccgca cgttcatgca ggttatccac gttttgggtc acgattccca
     8521 catcaaagta tttttccagt ccggcaagaa tctcatggcc cttgttgggc tgcacccctt
     8581 gcagggctgc ccggcgttcg ttataaaaat ccagcatgat ccccggattc ctgtaccatc
     8641 cttctatgga ggccacttcc tctacaggat ggttctccca aagccctccg ctgtcgcgaa
     8701 aagtgctgat gccgctttga gcactcattc cggctccggt caatactaca atttttttca
     8761 cgtcagccag tatgtcttgc agtatcgttc ttcaaaccaa aacctgaaca aaggatcaat
     8821 gaaacgtggt tttttacgga ctgtttcaat tatgcctttt ctttccaggg cgtcccgtat
     8881 ccgggaaaca ttgcccgaag atttcaggtg atacttatcc atcacctcta tagaacagaa
     8941 ttgttccact cccatgcaga cggcccgcag gaaataaatc tgcagtgtcg tcagttcctg
     9001 gcatattgat tcgaaggatt gttggaaaat gtcgagcagc tcttccaaac cactctgata
     9061 catgtcgtcg ttcatgtatc cttttgtgtt ggaaaaacaa atgtcggcca ggtattgaac
     9121 ataataagga tggccttcca ttttctggta gatcatccct gcgtaatccc tggatatcac
     9181 cctgcctgct ttggcaaaac tccggatgat gtaatctgtg aattcctttt cttctatggg
     9241 ttccagggga atatgttcag caaaacggta aaagggcctt cgctcatcaa agagttcccg
     9301 gaacacagaa taaccggaag aagccgtgct tccggacccg cagatgagcc acgtaaccgt
     9361 cttcatgctt ttccacagtt caaccatttt ttggaaaagc gggcgggacg gggttcctga
     9421 aaattccacc agggtctgga aagactccag gaccatgatg atgggtttgc ccaggtgtgt
     9481 tgaaaaagcc tgtggaaagc gagccagttc gatgtaggct tctccggaca ggtcgtcggg
     9541 aaaaaccagg aaaaattcat gttcctggtg attgggtacc gggattccgg gcttgcttag
     9601 cggcaacagg gtggcggcca aactttccca atcacccaat gtgtttgcca cacagctaaa
     9661 aagtgcgttt gccagcgcgg catacaagga atgttcactt ctgatattga agcaggatat
     9721 acgacagacc aggggagctt tttcgttttt gctgatttgt accagtacct tgttgatgaa
     9781 ggaggtcttc cctgttttcg gtttgtccca tacaacagaa tgctgtccct tcaaaaggtt
     9841 ggaggccagc cagtatgatc cggctgtcct tcccaggaca tcgggtgcct gaaggggccg
     9901 gttgtattga aaaggtgaat tcataacaaa gtggttacaa cgaacaaaga tactttttct
     9961 ttggtattcc gggaaacttt tttacctttg cagtccgttt tgaggaaaaa ttattgacaa
    10021 tcgttaagct attgaataag aggtgtttgt taatacccgc ttgcggttaa aactataaga
    10081 agttttttta tgtacgcaat tgtagacatt gcaggacaac aattcaaagt tcaggaaggc
    10141 tggaaggtct ttgttaaccg tcttgaggtt gaggaaggcg aaagcattaa ctttgacaag
    10201 gtactgctca aggatcacga cggaaaggta cagctcggaa aaccttacct ggaaggagct
    10261 gctgtcaacg caacggtcct gaaacactta aaaggtgata aagttctggt attcaaaaag
    10321 aaaaggagaa aaggacacca gaaatgcaac ggacaccgtc agcatctgac acaaatccgg
    10381 attgacacaa tttcataaca tctaaaacgt agtattatgg ctcataagaa aggtgtcggc
    10441 agttcaaaga acggccgcga atcacaaagc aaacgtttag gcgtgaagtt atttggcgga
    10501 caggttgcca aggccggtaa cattctggtc cgtcagagag gtacggtaca caatcccggt
    10561 gataacgtgg gtatcgggaa agatcatacg ttgtatgccc ttacagatgg aattgtggtt
    10621 ttccgcaaga aagccaataa caaatcatac gtttccattc aacccttggc agaaactgcc
    10681 caggcgtaag tccaacggat ctgcatttaa ggagactagc cataaatggt tagtctcttt
    10741 ttttacatct cttaagtgtg cgagatggcg atgcactcca tttctatgag ccaggcgggg
    10801 cgacagactc tgcccatgac gatgacggtg gggatggacg ggaagcggct gcgaaatgac
    10861 tcacgaacca gttcaaagtc tttctgcctt cgcaggtata caatgatctg agcgacgtct
    10921 tcaaaactgc tgcccgcttc ttgtagcagc acttccacat tctcccacat cctgtgcgtt
    10981 tgagcttgta tatcatccgg gtgcatcacg ttgccccatt tgtcaatgct ggctgtgccc
    11041 gagataagta cctgggtgcg gtcatccagg cgtagcgcca caccccgctc aaaggtgacc
    11101 ccgtaaatat tggtgggact caggttggtc agccccctga ggtatgtaat acgatcaggt
    11161 tccaggccac cgacagcata ggcgtccatg gtcacatacg aagcgggagc cccgttactg
    11221 ccttctatgc cggtgctggc aatatagtgc gtatccttgt ccatcccaat ctctgaaaaa
    11281 tattgtgtgc gggattgggc gaaagcagca taattcgtgt caatatcctt gatgaagaac
    11341 caggtcctga tgcattggcc cttcatgcca catcccttgg cctgcaacca ttgctggtaa
    11401 tccatgagca atttgcgtgt ctgttgggtc acattgcctg ccagacaaaa tttttctgta
    11461 gtccagaagt gggtatacct ttccggtaca ctccctttgt ctctcaaata gatccatacg
    11521 gccatttctg ttttgtttgc aggcggttgc tcaataaaag acacggcaca tgtggccggg
    11581 aacaactccc tggtatggat acgggaccgt tgcactgaag catctttcag gaaatacctg
    11641 acaaaaaccc gctctgaatc acggtaagga tcgctggaaa gccactcttg catgcaggaa
    11701 tgcaaaacgg ctatgcagtc agtgccggta tctgcaccgg gattatcatg ctgccttgag
    11761 aactgaaaga tcatgtggtg ttcggtcctt tcacccgacg ggggggtgaa agaggagacc
    11821 tgtaccgaac attccgggtg atttgaaaat atgtgatgcg aaaactccat gaccatgtaa
    11881 ttattattcc ggtgcaaaca ccccttcata aacagccctt ctgagggagc cgttttcatc
    11941 atctccctcg tattcccagt acatggcccc tttcattcca taatctttga taaagcggga
    12001 tttataacct atggagagcg ggttgtcata cgtgcacaca atctttccgt tgcagtcttt
    12061 caggtaaggg gccttggcga cgtcgtccca gcagtgtaca tattcttcca actgtatgag
    12121 tttcccgtag cctcccatgc cttttggaaa atccgggccg gtttttccat agaaaggtat
    12181 tcccaggaca agcctttcaa gggggaatcc tgccctgacg tgggccagaa ccgattcata
    12241 gcaggaaagg ttccctgtca tggaggaagg atacagaccg gaatgatgga aagggggcag
    12301 gccgatatca tacgtcatga tattcacaaa atccacataa ccggttatgg atttgaagtc
    12361 tatgaacttc ccgctggcag ctgtagctag tgtgagcagc atgccgtcgg gcaggtgttt
    12421 gcggatatcg cgcatcagga gcgtgaaatt cctggtgtct tcgggagcat aggaaatgcc
    12481 gggtccctgc ttggtgggat attcccaatc tatgtcaatt acgtccaggc cgaattcttc
    12541 cacaacacgc ttgcaatcca ttgcaaaaga gtctcttagg acgtcaaccg ccaccatttc
    12601 actaaaacgt ccgctacccc agccgccgat ggacagcagc accttgaggg atggtttttg
    12661 tttcttcaag gctacgatct gccggagtct gtcgggatta tccacgatca ccccgtcgaa
    12721 ggtttcattc acgtgtccga aggcatagtt gatatgggtt atgaaggcag ggtcgggaac
    12781 agcttctttc catgaagtga cataggcgac tgccaccttc tcttcaacag gcatttccgg
    12841 ggctaaaagc cactgtaacg cgttggtaaa aagcaggttc tgggatgcat ccgacaatcc
    12901 gttgcctccg tgtcccatgt tcatatatac catgcggtat tccctgtttg cccatgcaat
    12961 ggggagatct tcgctttcaa agatccttga tatgccgagg gggaaagatt cagcagccac
    13021 gctggccaga acctgaactt ttggattttc acgcaaaccg ggtgaaaaac ggtaccattc
    13081 gttggccggg gctataaaac tttccggtac acccttgcag accgggtgca gggggtcttc
    13141 cacctgtatc cgtgtcggca ggggtggcca gttgtaatct gcaaaaacca cacctcccag
    13201 gaacctggtg aaccagggcc attctccttc cctgtacaat gcggccttgt gaaagccaat
    13261 ccaccggccc ccattgtcca tgtattcctc gaatgctttt ctctgttttt tgtccagggg
    13321 aaagtaattg agcatcatga ccacatcggc atctcccagg ttttccttat tcatggccga
    13381 ggggtccgca gtggcttcaa acttcaactc acggcttccg ttaagcccgg aaaaaaagga
    13441 tatggcactt tctgcgaatg cgacgtgatt agcgtcctcg cccaggttat agaaggcaag
    13501 gatcctgagt ggctttaagg acggggcggc cgaagcagat aatgccgggg tggcggctgg
    13561 gaaaacagca ggggtggcta tgggagacct ttgagcatag gtggttatca ggcttgcagt
    13621 tgcaataacc aacgggaata ttctgcgtat gttcataatt gacaaaagtt tctttaccaa
    13681 aaatacaaaa aacaatgcat ctttgcagtg aaataattga aatatgcata ctgtactgca
    13741 aattctaaat cttgccggct ccctgggtct ctttctctat ggcatgaccc ttatgagtga
    13801 atcgttgcag aaaattgccg gagacaggct ccgtcgtatt ctttcctcca taacatcaaa
    13861 tcccctgaaa ggagtactta caggtgttgc catcacttcc ctcattcaat cctccagtgc
    13921 cacaaccgtg atggtggtga gttttgtcaa tgccggacta cttacgctca cgcaggctat
    13981 cggggtcatc atgggagcca atatcggtac aacggttacc gcatggatcg tatccctggt
    14041 gggtttcaaa gccgacatag ccattttggc catacccctg attgcggtcg gattcgtcat
    14101 gttcatgtcc aaaaagcaga aacggaaaaa caccggggag atgctcattg gatttgcact
    14161 attgtttctc ggcctcacct tcctgaaaga ttccatgccc gatctgagtt cgagccccga
    14221 gacccttaaa tttttacaaa attttaccgg acatggtttc tggtctgtcc tgatcttcgt
    14281 gggaattggc acactgctta ccatgatatt acagtcttcc agtgccaccg tggccctaac
    14341 cctggtcatg gtcaattacg gatggatccc gtttgagatc gcagcatcca tggttctggg
    14401 agaaaacatt ggaacaacca ttacagcaaa tattgcggct gcggtgggca atgtttctgc
    14461 aaaacgggca gccttggcgc atacgttttt taatgtgata ggagtgatct gggcagtggc
    14521 tttattccgc ccgttcctca gactggttgg agccataata accggacttg gcggggccga
    14581 tccattcatt cccattgccc aggctgcaga tccagtggcc tcggggaatg cgatgatcta
    14641 ttccgtatcg ctgttgcaca ccatgttcaa cctcattaac acgaccctgc tgatatggtt
    14701 tgtccctatt ctggtcaaaa tagtgacctt tgtgattaag agccccaagg aagaagaaaa
    14761 gtaccgcctc aaattcatcc aggcaggttt gctgagtacc gccgaattat ccattgacca
    14821 ggccaaacag gagatcatac atttcgggaa tatcattaca cgacaatatg ccattataag
    14881 gaatgccctg gatgtcaagg ataatgatga attcgacacc ttgttcaaca aaatttctta
    14941 ctatgaggaa gtgacagaca ggatagaatt tgaaatcgca gaatacctga acaagatagg
    15001 ggatgaagga atcagcgcgg agtccgggaa caggatccag tctatgtata agatcattgg
    15061 agaaatggaa agtatgggtg actcgggata taacctggga aggatactgc agagaaagcg
    15121 gaacaacaac gtttcctttg atgaacgcat gatccgcaat gttctcggga tgatggacct
    15181 ggtagacaaa ggctatgaga tcatgctatt caatcttcag gccgactata caaaactgaa
    15241 caacatagac aatgccctgg atgctgaaaa agatatcaac aaatatcgag atatgatcag
    15301 agagggcaat ttgttaagcc tgggtaaaaa tgaatatgat tatctttcag gcgtttatta
    15361 tatggacctt attgcagagc tagagcgggt aggggacttt ataatcaatg tttccgaatc
    15421 tatcatggaa attaaatcat gataaaatgt taacaattaa acttttaaga gaagatccgg
    15481 attttgtggc agaacggctg gccataagga acatcgatgc gcgtcctgta attcagcaaa
    15541 taatcagcct ggatacccaa aggagggcgg cacagacaga agccgatgcc ctgacggcag
    15601 aacaaaaaaa gatggcatcc cggatcggga agctcatggc agaaggtaaa ctagaccagg
    15661 ttgccgagat caaaaagaac gcaggagaaa gcaaggaaaa ggtaaaggct ctgatcgccg
    15721 atcaggaaaa gtatgaaaaa gaactgaacg agctgatggt cacgttgccc aaccttcctc
    15781 ataagatggt tgcaagggga gaaggagcac aggacaacgt cctggtgcgt gagggaggac
    15841 aaatccccac catgattcaa ggggcgctac ctcactggga actggccagg aaatacgacc
    15901 tgatagattt tgacctcggg gtaaagctca ccggggccgg tttccccgta tataaaggga
    15961 aaggagcccg tttgcagatg ggcctgatac aatttttcct ggaccagaat gccggcgaag
    16021 gataccagga aatcatgccc ccggtcatcg tgaacgagga ttccggctac ggaacaggac
    16081 aattgcccga caagggacag cagatgtatc acgtggggct tgacaatttt tacctggtac
    16141 cgactgccga agtccctctc accaacattt accgcgatac catcctggaa gcttccaagc
    16201 tgccggtgag gcttaccgcc tatacgccct gtttccgccg cgaagcgggt tcctacggca
    16261 aggatgtaag gggcttgaac cgcctgcatc agtttgacaa ggtcgaaatc gtacagctca
    16321 cgcacccgga tcattcctac tcggctcttg atgatatggt caaacatgta gagcggctgg
    16381 tacagatgct ggagttgccc tatcgcatcc tgcgcctgtg cggagctgac ctgagcttca
    16441 cctcggccat gacctatgat tttgaagtgt actctgcggc ccagcaacgc tggctggaag
    16501 tcagctcggt ctccaacttt gaaacctttc aatcaaacag gcttaaagcc cgtttcaggg
    16561 gagaggacgg caaaacccaa ttggtacaca cacttaacgg aagcgccctg gccctgccgc
    16621 gtatcgtggc tgccttgctg gagaataacc agacagagga tgggatcatc ataccggagg
    16681 ttttacacaa gtacctccat ttttccagga tataacaggt gaaaaacgga cagatcataa
    16741 taggcataga cccaggaaca cgtctaatgg gatacggcgt tatccgtatc aatggttcga
    16801 aaatggaaac actggccctt ggtgttgtcc ggatggcttc catcaaggac cattaccgca
    16861 ggatgagcca tatccttgag aaaatgtatg aattgctgga tgcgttccaa ccagactgca
    16921 tggccatcga agcgcctttt tacagcaaga acgtccagtc catgctcaag ctgggaaggg
    16981 cccagggagt ggcgattgcg gcggctctgt cccggaatgt ccccgtctgc gaatatgccc
    17041 cccgcaagat caagatggct gttaccggaa ggggagcggc ttccaaggaa caggttgccg
    17101 ccatgttgaa acatatgatg cacttcacac aggagttcga gtccctggat gccaccgatg
    17161 cccttgcggt tgccgtgtgt cacgcaaatc agctgaaatt acaggagaaa acggctccgg
    17221 ccggttcctg ggaagctttc gtgtctaaga atcctcaaag gattgtgagg aaatagaata
    17281 ccttcgccac ttatccaata cttgtttcat gtcatcgggt aacggtgcct ggaaatgcac
    17341 cttttcccct gtggcgggat ggatgaaacc cagcgttgcg gcatggagtg cctgcctggg
    17401 caggatttca aagcaattat ctatgaattg tttgtatttg ctgtagagtg tccctttgcg
    17461 gatgcggtcc cctccataac gcacgtcacc aaagagaggg tgcccgatat gttccatgtg
    17521 tacccttatc tggtgcgtcc ggcccgtttc aaggcggcat tccacgagcg taacgtagcc
    17581 aaaccgttcc agcaccctgt agtgggtaac ggcatgtttg ccattcaccc cgtcggggca
    17641 aacgcggaac cggagtctgt ccgagggatg gcgcgcaata tcgcctgtta ccgttccctg
    17701 gtcgctttcc atatctcccc aaaccaaagc cacatacaac cggtctgccg agtggtcgaa
    17761 gaactgcttt gccagcctga attgagccac ttctgaacgc gcaaccacca aaaggcccga
    17821 ggtgtcctta tctatccggt gtaccagtac ccccatgcgg gaatcgtccg ttttgaccgt
    17881 tccccgttcc ccaaaatgcc aggccagtcc gttgagcagg gtcccggtgt aattgccatg
    17941 tccgggatgc accgtgagcc ctgctggttt attcaccacc agtatatcct ggtcttcata
    18001 cacgatatcc agggggatat cctcggcgat aagacggaat tccctttttt caaaggggag
    18061 catgatccgg atatcgtcca ggggctttac cttgtaattg ctttttgccg gtgtcccgtt
    18121 aaccagtatg tattcagatt cagccgcaag ctgtatgcgg tttcttgaga tggcttccag
    18181 ttttgatgtg aggaacctgt ctatgcgcaa aggggattgc cccttgtcaa cgacaatatg
    18241 aaaatgttcg aaaagttcct gttcatctct catttacagg tcttcttctg taatggtgat
    18301 atcccggggt atgagcaaat cgggattgat ggtaaggacc aggtgtattg aagtacccag
    18361 aggatatgcc ggttgatggg aaaaatcggg ggattgactg aaaacacgtg cagccagggt
    18421 gtcggcggca gtgaggaccg tcccgtcata cttgatgctc ccgatattca gtgaatgatc
    18481 gcttatgatg tcaagtgctg cttccaggct tttccccgtc agcatgggaa tgtatgccag
    18541 ttcgtggccg ggtgccattc ccagtttcag atcaatggca ctgtatatgt tgataggagc
    18601 accggcaggt aagggctgtc cctggtacat ttgttccagt acgttgttgg tggctaagtc
    18661 gggaacgtaa atcagacgcc cgagctttag tccatgcgaa gccagttctg cttttgcctg
    18721 ccttagcgag aaaccaacca gggaaggtac agatacctgg cggggcagta atgaattcac
    18781 ggtcaattca atcctgcggt tctttttaac atgctgaccc ggaggaggaa cctgtctgaa
    18841 aatcactccg gcaggcaatt caggcatgta gagtgaatcg gttacttcca ggcgcagatg
    18901 caaaggtctg gcagttttcc tggcttcttc catagagaga ccggaaaagt cagggaccag
    18961 taatcctttc ccgtgacgtg tgaagaggtg taaaaaagta tatatgccaa gaaaaagcac
    19021 aaccagaaac acaaaaatcc acagtaagtt cctgaggata ggtttcctta tggttttttt
    19081 accaggcact tttagaaacg atcttcgtcg gatacagtga gtttttttcg tccgcggagg
    19141 cggcgggcag ctaaaacgcg acgtccgttg ggggtagaca tacgctcacg aaacccgtgt
    19201 ttgttacggc gtttgcgttg agagggttga aatgtccttt tcatattatt tgatacttta
    19261 actacttttc aataggggag cgcaaaggta cgaagtttgt ttgatttcac aaattttttt
    19321 ggtttcaaaa aagtcatctt ccagtcccct gtaagtgtct gcaagcggtg tcaccgaaaa
    19381 acagcttgcc ggtatgcggc atttgatcgc agcctctttt atttcagcct caagatcacc
    19441 ccccttgaga aagagtatcc ggcccggttc cctgagtttg ttccttgtcc agggaatgaa
    19501 tccggccagg cttgtcacag cccgtgaaac catcacatca caacacctgt cgggcatttg
    19561 ttcaacacgc gaaaccacgg cggtcacatt atccaggccc agcgaatccg agatctccct
    19621 cacaaccttg attttttttg caatggaatc acacaatatg aaccttgact gcgggtgcaa
    19681 tatggcaagg gggattcccg ggaaacctcc ccccgtaccg gcatcaagca cagtggcacc
    19741 ggcctggaag ggatcatctt tgatagcggc cgaaagcgtg agcgaatgaa gtacatgcct
    19801 tacatagagg tgctccaggt cctttcggct gatgacgttt atgcgctcat tccagaatgc
    19861 ataaagcccg gccagttcca tgatgcgttt cctggcagcc tggtccaggt gggggaaaaa
    19921 tcgaaaaaca acgtcggcat ccatgtcact ttttgctctc cagctcaatg atcctggcca
    19981 ggctcacctt tgccctgtga attcttgttt tcacagtgcc cagcggaata ttcaattcgt
    20041 tggcgatctc ttcgtaggca taatgatgca tgtaacgaag acggatcact tctccgtatt
    20101 ccggcttcag ccgtgatatc atgttttcca tgatctgttt ctgttgctct gctatcagga
    20161 cgtcttcggg gttgacatcc tcggcatcct gcgcttcttc aagcgaaacg aaaaattgtt
    20221 tgttcttcct gaaatggtct atgcacagat tctggacaat gctgtagagc caggttgaaa
    20281 aagcatattt tggattgtaa ttcttaaggt tcctgaaagc cttgtcaaaa ctcatcagga
    20341 ccaggtcttc cgattcggag gaattgatta tcagcttgtt gatgaacgcc tgcacgcggt
    20401 ccttgtgcct gaccagaagc cttgtatagg cttcctgcct gttctgcagg gccagttcca
    20461 ccagggcccg gtcatccaac tggtccaaag attgtgacat aagatcagtt cttaagccgt
    20521 ttgataagcg aggcaacaag atagtaaaaa tgtacaagag gagaaaacag gtcccataca
    20581 ggggcagcat aagcaagcct tttctcatca agatgcaatc caaatccgat ataatgcatc
    20641 cagacagtga tccagcgaag cagcagcaaa cctcccggca ggatcatcag cagggagtct
    20701 cctgtctttc ctatccttac acaggcaaaa gccaggagga tgtaaaacgc ggctatgaaa
    20761 accttctcca gggtcagtaa aaaccaggcg cgtttttttg tcagcatcaa ggtacataac
    20821 ctttctgtcc ggttatgatg gtactcttga agagtgattt tgcggtccag gactactgtt
    20881 ccacaaggat agacacagga gtgcgtgttg tttttctcca aaacatggga tatgatggcc
    20941 tgctcgcttt ggttgaaggc ggtcgtactg gtttcaaact ccctcccgtt gaggaatttg
    21001 tctttttgaa aaagtatgtt gttcccccat gaaacataag gcatcttcct gcgcgtggcc
    21061 cccatctggt gcagttgctg gtccatcatg tcataacgca caagcaggct gctccctgta
    21121 aaggaggtat accccatgac ggtatccgct gttttttcca gggctgtctt tacaagggaa
    21181 acgagccatt ttttggaaga aggaaagcaa tcgggtctga aaaagacaac cgacgggtac
    21241 ttcgcagcac gaatacctac tcccaaagcc atcagttcat cccgcccgaa ttttgcattc
    21301 gcgctcaaaa tcctaacagt cagattttta tgaaagtgtt ccagagaggc cagcaacacc
    21361 tctgtatcgt cctcggaatt cttgtccacc acaacaattt caaaggtttc gtattcctgt
    21421 tccagcaatg caggaagcaa ccgcaccagg ttatcgtatt cgtttctcac acaaagtaca
    21481 acggatacac ccggggcctc ttcttttacc acagggttcc tgtccctttt cccgaatgcg
    21541 ggacgcgcgt attgcacgaa cagaaacagg gtgatcacga caaaagaacc tagtgtgagt
    21601 atatacaaca gcggttcctg gtataacagt gttaaatcca tggttatcat ctaatgcgcc
    21661 tcctcccagt tttctccaaa tccaatgcca accgacaggg gaacactgag cggggctatg
    21721 ttttccatgt gcgaacgcac caattgttcc atgatctcct tctcggggcg gtaaacatca
    21781 aaaagcaact cgtcatgcac ctgcagtatc atgcggcttt tcagtccttt atcccggagg
    21841 gccctatgca cagcaaccat cgcttttttg ataatatcgg ctgccgtacc ctgtataggc
    21901 gcgtttatgg cgttacgctc ggccagtccc cggagcatgg gattgggtgc cttgatgtcc
    21961 ggcgcatagc gcctgcggtt gaacaaagtg ctcacatatc ccttttcttt tgccgaggcg
    22021 attgtactgt caaggtactc tttcactttg gggtagtgtt caaaatatcc ttcaatcagc
    22081 tcactggctt ctttcctggg aatcctgagc ctcgaggcca gaccgaaagc ggaaattccg
    22141 taaatgatgc cgaaattggc cgtcttggct ttgcttcttt gttccggcgt cacttcactt
    22201 ttgctgcaat ggaagatctt tgccgcagtg gccgtatgga tatcggcacc ggcatgaaag
    22261 gccgcgatga aatcttcatc accgctcatg tgggccatga tccgcagttc aatctgggaa
    22321 tagtcggccg acatcaggat gtggtccccg tcgcggggaa caaagaactt ccggatctcc
    22381 cggccccgtt cttccctgat ggggatgttt tgcaggttcg gtttctgtga actgagcctt
    22441 ccggttgccg taaccgtctg gttgaaagtg gtatggatgc gtccagtcac aggatccaca
    22501 agcggcggga tgctttcgat ataggaggag agcatttttt tcaaagacct gtattccaga
    22561 atgaggggta cgatggggtg ccggtctgcc agcgctccca gggtttcttc gtccgttgta
    22621 tactgttttt tgcccttgcg ggagcggtca tctatcttga gcttgtcgta caagatgatc
    22681 cccacctgtt tgggagagga cacattgagg gtggggtcgc ccgcatgctc cctgatctgc
    22741 cgttctattt ccagggccga tttgttcagc tcgctcccgt atttttccag ttgccggata
    22801 tccaccctga ccccttcctt ctccatggcg gccagcacct ccatcagcgg catctcaagc
    22861 tccgtataca gggtttccat agcatcgttg cgcagcacct ccaggagagg ttctttcaga
    22921 tggaacgcta tgaaggcctc gttcaccagg ttttgtatgc caggatcgtc ttcgggatgg
    22981 gcgtcgaaaa gactccggct ttccatgggt ttctcagccg gaaaatagtt gaggtattgg
    23041 atggccaggt cggcaggccg gtggtttctt tccggattca ccaggtagtg catcacatcg
    23101 gggtcccaga gcataccctc caggggaatg ctttgcagcg acaggggcaa catcaggtct
    23161 ttcagtttga aaccgcattt tgggatattc ctgttgccca gcaacggggc agccagtgcg
    23221 gcggtggtca aataagcatt gttcccggca aagagtacga gttgctgagg acttcctgcc
    23281 agccctgcct tcccttgcct gagtgcttcc tttatgatgg tttccactac agcgggattg
    23341 tctgcaggaa tgatactccc gggcggggag agggaaacgg aaacagcttt gggagcctgt
    23401 tccccgttgc agcagaacag ctcttccagc ctgggcagca tgcgggacag cgaatgaaat
    23461 tcgtattccc ttaacagttc tttcaggcgt accaggtcgg gtgcgtttat cctgaaggat
    23521 tcctcgttcc agggaatgtc cagcccggta cggatggttg ccaggtcttt agacaggaag
    23581 agcgtctcac gcgactggtt cagtttatcc tgcagggaag aagacagttc cccaaggttt
    23641 tccaggatgt tttccacgga accgtagcgg gacaccagct ttttagctcc cacttctcct
    23701 atgcccctga cacccggtat gttatcggaa gtatcccccc agatggccag gatgtcaatc
    23761 acctgccccg gatcgctgat gccgtatttt tcacagattt ccttcacgcc catgatctcc
    23821 cagccatcgt tgtggttcaa gggacggtac atcaggatgt tttccgatac aagttgtcca
    23881 tagtccttgt ccggagtaac catgaagacc ttgaaccctt cattttcggc ccgcttggaa
    23941 agggtgccga tgacatcatc ggcctcggct ttttctatgg tgagaacggg aatcctgcac
    24001 gattccagga attcccgcac cacaggaagg gaatccttta tcacctgcgg tgtttcgggg
    24061 cggttggcct tgtattcggg gaatgcttcg tgcctgaaat tctttcctcc cgggtcgaaa
    24121 gccaccagca ggtgggaggg tttttctttc agtatgatat cgaacaggga gcggcaaaaa
    24181 ccgtaaatgg cagatgtgtc tactcctttc gtgttgacca tggggcggtt gagaaaggca
    24241 aaatacgccc ggtagagcag ggcgtgcccg tcaatcatga atattcgttt catttcaccc
    24301 attctgcata agatgtgggg gtttcataca atcggacgcg acagaggcgg ttgggcggcg
    24361 tgatgaccgg ttccaggatc ccggcaatgt aacggaccag ctgttcacag gtaggctgaa
    24421 aatcaaaaat gataacattg tcataacttt ctgccagttc cctggcaagc ggcgcgtctt
    24481 tgcgcagcat cagggagtgg tccaggggtt cgagaacatg ccgggtcacg atctgtttca
    24541 agtcgttgaa atccatgatc atgcccgatt tgggatcttc gggaacaagc gacggttctc
    24601 ctgtgacggt aaccatcaag cggtaagaat gtccgtgaat atgacggcat ttcccatcgt
    24661 aatgggtcag cgcatgtgcc ccctcaaagc gaaattctct tgtaatatgc agtgcagaca
    24721 tagagcgcta ttaattacgc aaatgtaaga tatttatccg agagcagctc ctaactaatg
    24781 attttattaa taatgacagt tattgctata taaatgacat tttttttgca tttatggtta
    24841 aacttattta gttttgcttt aattaaaaac atcatattat gcaacagaat tacttagatt
    24901 ccatcatttc cgataacgca aagaatatga ggcgttccgc catcagggac cttctgaaaa
    24961 tggccacacg tcccgagatc atctcctttg gcggagggtt ccccgacatc aacgccttca
    25021 atgtggacga actcaatgag gtgatacagg aagtaatgag ggagaatgcg ttgcaggctt
    25081 tgcagtacgg ggaaaccctg ggggtgaaaa ggctgcgcga agcgctggct gcccactacc
    25141 gtaaacaggg catggatgtg acctatgaga atttcatcat taccacatct tcccaacagg
    25201 ccatagacat ggtaacccag ctcttcatca atcccggtga tacgctggtc tgcggccttc
    25261 cttcgtacct gggagcgctc caagcttttt atgcccacag ggcaaatccc gtaggcattc
    25321 caaaagacga ggaattgcgt acggtggtta acgcactggt cactgccgga aagaagccca
    25381 agttcatata tgccattccc gatttccaga atccttccgg ggtgaccatg actttgaaac
    25441 aacgcctgaa cgttcttgag gtggcaaagg aattcgatat cctggtgctg gaagacagcc
    25501 cttaccgcga gatccgcttc agcggggaac ccatgcccct gatctactca ctggacaagg
    25561 aaggcaggac catgttgctg ggcaccttct ccaagacatt cattcccggt ttccgcctgg
    25621 gatgggtcat ggccccgccc gctatcattg aaaaacttga gatcgtaaaa caatcaacgg
    25681 acctgtgcgc cccggtgttc aaccagttta tagccgcccg ctacatggag aaaggctact
    25741 ttgacaagaa catagaacgc atcaagatca attaccgcaa caaacgggac aatatgatgg
    25801 ccgccttcgc aaaatacatg cccgagggcg tgacctggac aaaccccgaa gggggactct
    25861 tcctttttgt caccctgccg gagggttatg atgccaccga attgttccgc atagctttaa
    25921 agaaagatgt ggcgtttgtg attggtgagg ccttccattg cgatggctcg ggcaagaaca
    25981 ccctgcgtat caacttctcc ttcacggaag aatccagggc agtagaaggg gtccgccgcc
    26041 tggccgattc cattcgccag atgatggagg aaaggaaagc taaaggttga tgatcaggtc
    26101 ttcaagcgaa cgcttgggga catgatgcac ctctgcttca tccctccagt atttgatact
    26161 ttccccggcc ggcaggccgg ggtttatttt ttccaggacg attgtctcgg ctggtttgcc
    26221 aagagccacc acataaagga tctccagatg ctcgggcagg gacaggtatt gttgcagccg
    26281 cggccggttc acagaagcaa agatgcagcc tgcgtagccc aggtatacgg cctgtagcag
    26341 gatgctctgc agggcaatgc ccggatcgca aagcacttgc tgatggatgg tcttgtcgtg
    26401 tagcatgatt atgtacgctg caggcctctc tccttcggca ggacccggcc agtccttgag
    26461 ataagctgcc caggccaggc attcgaaaat ttctttgccc cttgccgagt ccgcctcccc
    26521 gcacaggatg tacttcaagg gttgcctgtt ggcagcagac gggctcagcc gcgctgcctc
    26581 tatcatggac tccagttcag gaatcccgat tttcacgtcc tgcacaaacc tcctgcagga
    26641 cctgtttttc ctgatataat caagcatgtt catcatatgg cattcattaa tacagacggt
    26701 caataattcc ctgctctttc atcacggcag gcgttccccg gtgcaattca cgctccggtg
    26761 tcattatcca gaaaaagtcg caatgctcca tggccagaga caggtcgtga gtcgagaaca
    26821 ggataccctt gtcttcttcc tgggcgagtt tgcgcagtaa cagccctatg gaacgcttgt
    26881 tggcaatatc caggaaagcg gtgggttcgt ccagcaggat aaggggagta tcctgtacca
    26941 gggcacgggc aatggccacc ctgtggtact ctccgtccga aaggctggcg ctgttcctgt
    27001 ctgccaggtg tttgatgcct gcccgctcca gcacgctctc aactattagt tttccctccc
    27061 ggtcttccat gcccagccag ttggtatacc ggtaccggcc tgtgctgacc gtatcacgga
    27121 cagaaaaatg cactgcccgc aagggttgag agggtacaaa agcacagaaa tcattcttct
    27181 tcacaccctc accgtgcctt atggagccct tgaggggagg cagcagcccg gcaatggttt
    27241 tgagcagggt ggacttcccg atcccgttgg gtcccaggag cccggtcacc gtaccgctgt
    27301 ccagttgcgc gttgatgtgc tcaaagagcg gtttatgcca ggaatacccg accgatacat
    27361 ccaatatgtc cagaattccg ctcataggtc gacagccctg tttttgaaga tcacatatat
    27421 aatgacagga atgcccatca gggccgccac cgtgttgacc ggcaagacgc tctgcgttcc
    27481 cggcaactgt gatatgatat cggccagtat catcacacag gccccggtaa gggctgaggc
    27541 cggtatcatg acgcggtggt ttgcgttgcg gaaaagcatc cgggccatat gcggcacggc
    27601 aatccctata aaacctatgg ggccgcagaa tgccgtcacg cttcctgcca gcagggtggt
    27661 tgccaggaaa atcctgttcc tgacccttga cagggagatg cccaggtttt gggcatactg
    27721 ttctcccat
//
LOCUS       DAOSOF010000006        25356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1964_length_25356_cov_5.400972, whole genome shotgun sequence.
ACCESSION   DAOSOF010000006 DAOSOF010000000
VERSION     DAOSOF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1964_length_25356_cov_5.400972"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..362
                     /locus_tag="PLM68_00720"
     CDS             27..362
                     /locus_tag="PLM68_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOT53927.1"
                     /translation="MTVKEMAAALQLRVLAGEEGLEKQIAGGYTSDLLSDVMGYADAG
                     MVWITLQTHKNIIAIASLKELSAVVLVKGFEPDADTVALAIQEQITLLGTTEQAFEFS
                     GRLYRLLYP"
     gene            365..1087
                     /locus_tag="PLM68_00725"
     CDS             365..1087
                     /locus_tag="PLM68_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT53928.1"
                     /translation="MICAKADLHIHTFLSPCGNIGMTPLNIVAQAKAKGLQLIAVTDH
                     NSTLQGPVVRQVCEREGIAVIYGAEITTREEVHCLAYVGSEEQRVELQHYLEKHLPHV
                     PNNPDIFGYQLWVDEHEQVLGEAPYLLISAIDQGIEQVAAFVHSIEGLFVPAHIERSR
                     NSLLSQLGFVPPGLPADALELSRYTNPVDFCSKNATLKKYRIIQSSDAHFPGDIGTAA
                     TSFLMEKTGFAGLKTALTSTHE"
     gene            1080..1634
                     /locus_tag="PLM68_00730"
     CDS             1080..1634
                     /locus_tag="PLM68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT53929.1"
                     /translation="MNDLALHLLDIVQNSLVAGASFITIEVNENPAGNLLTISVKDNG
                     RGMTPEQVQKLSDPFFTSRTTRRVGLGVPLLRQTAEQSGGSLEVVSEPGKGTLLTVCF
                     QYDHLDRPPLGDVANAVVLMISANPEVGFSYHYAYNDRSYEVNTMELKEVLGDLPVND
                     LYVMEMIEEMIKENQSDLKVCQIE"
     gene            1692..2081
                     /locus_tag="PLM68_00735"
     CDS             1692..2081
                     /locus_tag="PLM68_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT53930.1"
                     /translation="MTKIKSLDDLRQRKQQLEQSMEIRENSDHPDSLVQIKVAMATCG
                     IASGARAVMNAFVEKIKKEKIPAVVTQTGCMGYCYAEPTVEVKKPGMDPVVFSYVDVA
                     KAEEIVDKYIRNNELLDGILPANYQTI"
     gene            2115..3893
                     /locus_tag="PLM68_00740"
     CDS             2115..3893
                     /locus_tag="PLM68_00740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT53931.1"
                     /translation="MQLLVCGGTGCRASASAKIVEKLNESLASHGLTQDAQVVTTGCF
                     GFCEKGPIVKVIPDNTFYTQVKPEDAEEIVAEHILKGRKVKRLLYENPETEEHIPDSK
                     HMGFYQKQLRIALRNCGFIDPENIDEYIARDGYSALAQVVTELTPQQAIDMVKQSGLR
                     GRGGAGFPTGLKWEITKNFTSDQKYVVCNADEGDPGAFMDRSILEGDPNTIIEAMAIC
                     GYCIGASKGLVYIRAEYPLAIKRLQIAIKQAKEYGLLGDNILGTNFSFDIELRYGAGA
                     FVCGEETALIASMEGKRGEPRVKPPFPAEDGYQHKPTNVNNVETYANVPVIFLKGPEW
                     FSSIGTAKSKGTKVFALAGKINNVGLIEVPMGITLREIIYEIGGGIKNGKKFKAVQTG
                     GPSGGCLTEKHLDTPIDFDSLVAAGSMMGSGGMIVMDEDDCMVSIAKFYLEFIVEESC
                     GKCTPCRVGNKRMLEILEKICQGKGNMEDLDHLRNFANVIKDTALCGLGQTSPNPVLS
                     TMENFYDEYLAHVKDKRCIAKKCKALATYTIMPDVCTGCSVCAKNCPVEAITGERRQP
                     HFINQNKCIRCGVCYTKCKFNAILVQ"
     gene            3902..5665
                     /locus_tag="PLM68_00745"
     CDS             3902..5665
                     /locus_tag="PLM68_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT53932.1"
                     /translation="METVKLTIDNKHVEVPKGTTILQAAKQAGINIPSLCYMNLPHLA
                     QEHKPGSCRVCVVEIPGKKTLVPACVTKCTDGMEVLSHSLRAINARRTVVELLLSDHP
                     KDCLICPSSGNCELQKLSINMGIREINSVENAAISTYRKDSSPSLLRNMDKCVMCRSC
                     ETICNEIQTVGALSAVNRGFMSVVAPAFEQDLTDSPCTFCGQCVAVCPTGALTAVDNT
                     FHVIRALADPTKTVLIQTAPAVRAALGEEFGYTPGTLVTGKMVAALRALGFDKVFDTD
                     FAADLTIMEEGTELLNRISRHLSGDKSVRLPILTSCCPAWVNFFETNYSDMLDVPSTA
                     RSPQQMFGSIAKTYLAEKMGIKRENMVVVSVMPCLAKKYECQREEFKVDGNPDVDYSI
                     TTRELAHLIRMGNVDFKSLKDEDFDRPMGESTGAGVIFGATGGVIEAATRTAYELFTG
                     KKLEKVDFYELRGMDGIRTATIDFDGTDIHIGIAHTLGNARKLLDGIRKGMYNFHAIE
                     IMACPGGCIGGGGQPLHRGDSIVLKARMQAIYKEDLNKVIRKSHENPEIARLYEEFLG
                     KPLSEMSHKLLHTHYFSHPKK"
     gene            5687..6160
                     /gene="nuoE"
                     /locus_tag="PLM68_00750"
     CDS             5687..6160
                     /gene="nuoE"
                     /locus_tag="PLM68_00750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT53933.1"
                     /translation="MKFEINDCHKKTIKEICDSYGNKRSELINILHDTQEHFGYLPEP
                     VQLEIALNLNMPLAKVYGVVSFYSFFTMTPKGKYPISVCLGTACYVRGAEGIVEAIEK
                     ELKIKVGQVTEDGKFSLDCLRCIGACGLAPVMMIGDKVHGRLEPEMIKNILSQYE"
     gene            6256..7347
                     /gene="prfA"
                     /locus_tag="PLM68_00755"
     CDS             6256..7347
                     /gene="prfA"
                     /locus_tag="PLM68_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT53934.1"
                     /translation="MPENSLLNRLEGIRRKFESLQQQLSDSSLIQDMKRYVALNKEYA
                     GLEPLVRAGDKYRTVIRNLSSAKELLLTEKDEELREFAKEEIDRLEEEIPEMEQHIKF
                     LLIPADPQDSKNAIVEIRAGTGGDEASIFAGDLFRMYSKFAEKNGWKLDITGLSEGTS
                     GGYKEIIFKISGSGVYGILKYESGVHRVQRVPQTETQGRVHTSAASVVVLPEADEFDV
                     ELNEKDIRKDTFCSSGPGGQSVNTTYSAIRLTHIPTGIVVQCQDEKSQLKNYEKALSE
                     LRTRIYNMEYEKYLTSIAAKRKTMVSTGDRSAKIRTYNYPQSRVTDHRINYTTHNLPI
                     FMDGEIHEVIDLLQVAENTEKLKDEPSNT"
     gene            7328..8089
                     /gene="pyrF"
                     /locus_tag="PLM68_00760"
     CDS             7328..8089
                     /gene="pyrF"
                     /locus_tag="PLM68_00760"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT53935.1"
                     /translation="MNRQTLNRLILEKRSCLCVGLDISSISFEPIRRIVEETAPYCVA
                     FKANTAFFEAHGWKGWKCMEETVSYIRSRFPDHFLIADAKRGDIGNTAVQYARTFFER
                     MDFDAVTLSPYMGYDAVEPFLAYRDKWVILLALTSNTSAADFQFSLTPDGAPLFQKVI
                     EKANTWAGRDKLMYVAGATRPDMLRLVRAMAPDRFLLIPGVGAQGGTVEEVMQHAKTP
                     QGDILINVSRAIVQSEDGPGIAAKEFAGSIARFFS"
     gene            complement(8020..8679)
                     /locus_tag="PLM68_00765"
     CDS             complement(8020..8679)
                     /locus_tag="PLM68_00765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT53936.1"
                     /translation="MFSLHIIRFDEIDSTNTEARRNLEHAREGTVWTASFQTNGRGQY
                     TRRWESVKGLNLLATLLLRPVFLPANKQFLISKCTALAICDFLVTLGLSPRIKWPNDI
                     YIGPNKICGILIEHQLSGNNLTSSIIGFGINVNQEQFGGAPNPTSIVLETGIRYNVDE
                     LLPKVLLHIQKWYQTLYDGDSGKIDRSYEKLLVNEPDYEKNRAMDPANSLAAIPGPSS
                     D"
     gene            8733..9071
                     /gene="rsfS"
                     /locus_tag="PLM68_00770"
     CDS             8733..9071
                     /gene="rsfS"
                     /locus_tag="PLM68_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT53937.1"
                     /translation="MKEVEIIAGAMLDKKAQDVSSLDLRKLGTTICDYFVICNADSGV
                     QVAAIADNVEEQMILKAGRKVRRSQGKENRFWVILDYSDIVVHIFQTEYRRFYHLEDL
                     WADAVTRHYT"
     gene            9088..11079
                     /gene="ftsH"
                     /locus_tag="PLM68_00775"
     CDS             9088..11079
                     /gene="ftsH"
                     /locus_tag="PLM68_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT53938.1"
                     /translation="MNGNFPNKNKPNKSPLLQPKRRFNPFLWVYVILIALIVYFMFAN
                     RSGEPVKTEWITVKEQMGPGGEIEKIVYISNMQRAELYIKPDALEKYKAKFGDKVPKF
                     APQFYFIVTENFDAEEQFEVLRNSLQEEQRFKIEIEQRTSYWGRIIDWLLFPALLIVM
                     WIFMFRRMSRGSEGGSGGGGIFNVGRSQAKLFEKDKTVANVTFKDVAGLEEAKVEIME
                     IVDFLRNPGKYTNLGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEM
                     FVGVGASRVRDLFRQAKEKAPCIVFIDEIDAVGRARSKNTGFTSNDERENTLNQLLTE
                     MDGFGSNSGVIILAATNRADVLDKALMRAGRFDRQIHVDLPELKERHEIFKVHSQGLR
                     LEEGFDLGFLAKQTPGFSGADIANVCNEAALIAARKNKKEIQKQDFLDAVDRIVGGLE
                     RKSKIISPQEKRTIAFHESGHATVSWMLKHANPLLKVTIIPRGRALGAAWYLPEERQI
                     TTVEQMMDEMASALGGRAAEELMIGQISTGAMNDLEKVTRQAYAMVAYFGMSEKIGNV
                     SFYDSTENAGFNLGKPYSETTAQLIDKEVRRIIDEAYDTARKVLKQNNEGFLKLANQL
                     LDKEVIFADDLEAIFGKRPSVETPQEETINATENSEHVN"
     gene            11063..11902
                     /locus_tag="PLM68_00780"
     CDS             11063..11902
                     /locus_tag="PLM68_00780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT53939.1"
                     /translation="MNTLIKRSLSGLVFLILMIAGLLLHPFGYAILMTACVGIMVIEY
                     YRMSLGSWNKAGQIFGFLSAVMIFLGTYIMSRYGVWTARYFLLFLPLPVTVTWITVLF
                     NKSENVFQYAAYLFLPLVYIAVPFSTFNFLVFNSLGMFQGMYLLVLFILLWVSDVGGY
                     LIGMGFGQKNGHKLYPRISPKKSWEGLAGSLVFSVATGLLLWKLEMLPFHWTHCLILS
                     LIISILGVVGDLTESAMKRHFGVKDTGHIMPGHGGLLDRFDGALIAMPIAIVYVLFVI
                     FKG"
     gene            11906..12568
                     /locus_tag="PLM68_00785"
     CDS             11906..12568
                     /locus_tag="PLM68_00785"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOT53940.1"
                     /translation="MMRIHKEGQSTILVVMSVVTIFLVVILAFSPYNIWVLASFILLS
                     VLLLVQTMMFFRIPSRNILIRDDGIVSPADGRIVMIQEVQVNEYLQEKRLQVSIFMNL
                     FNVHVNWYPCGGDIVYYKYHPGDKMVAWHPKSSDKNEHTTLFVKQDNTLIGVRQIAGL
                     MARRVVCKARTGKQVLQCSEMGIIKFGSRVDVLLPPDAEVKVRKGQRVWGCETLIATL
                     KR"
     gene            complement(12558..13199)
                     /gene="rpe"
                     /locus_tag="PLM68_00790"
     CDS             complement(12558..13199)
                     /gene="rpe"
                     /locus_tag="PLM68_00790"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT53941.1"
                     /translation="MGKVAVSMLSADFGHLADSCRMVNQSIAWSIHLDVMDGVFVPNI
                     SYGFPVIQAIARYARKPMDAHLMTVEPSRYYERYRELGVEWLTVHYETCPHLNRDLQE
                     IRRLGMKAGIALNPATPVSVLEQAVYSADLVLVMSVNPGFGGQAFIEESLEKINRLDR
                     IRKEKGLDFLIQVDGGINDSNARILYENGADILVAGNYVFKSPDPLAAITAIS"
     gene            complement(13201..14649)
                     /locus_tag="PLM68_00795"
     CDS             complement(13201..14649)
                     /locus_tag="PLM68_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT53942.1"
                     /translation="MNTPEPYSVFGFFFELCRIPRESGNESGVTRYLEQFAKDRKLDY
                     QTDKTGNVVIRKAASAGKENSPCAVLQSHIDMVCEKNADTVFDFSKDAIQYYIEDGWL
                     RARGTTLGADDGIGVAASLAVLDDDSLEHGPLECLFTVSEEIGLDGARAVAPGFIKGT
                     ILLNLDSEDEGEVFIGCAGGIDTIACFSYHSIPTLPTGLTALSVSISGGTGGHSGDEI
                     HKDLCNAVQVLARFLWKAQQHFSVDISSLSGGNKRNAIAREASAVCVIRKEDEASFRE
                     LFSHMAADWEKEYKHTDPELQSQILSCQFPDAVIERKTARNLVSALYSCPHGVLAMSK
                     DVPGLVETSTNLASVKMEPDNHLIRIGSSQRSAINSARRNAASRIESLFLLAGAEVTH
                     QGEYPGWAPNPDSPTLKICVQAYQELFNTPVKVKAIHAGLECGIFLDAYPYLDMISIG
                     PTLKGVHSPAERLDIESTAKFWALLLKILKMV"
     gene            complement(14661..16076)
                     /gene="dnaA"
                     /locus_tag="PLM68_00800"
     CDS             complement(14661..16076)
                     /gene="dnaA"
                     /locus_tag="PLM68_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT53943.1"
                     /translation="MAPSPHKIVWNNCLNIIRDNIQPSSYNTWFEPIIPLQLKNNVLT
                     IEVPSHFFREYIEEHFIDLLGHSLRKELGSMAKLEYCVKVDSHSKGMPLPQQGQTQFQ
                     NKAIPFSRINRDEVASPYVIPGIRQVKIDPQLNPAYSFDNFVEGSCNRLARTAGLSIA
                     DKPGGPFNPLFVYGGSGLGKTHLGQAIGIEIKKRMPEKIVLYVSANLFQIQFMDAVNV
                     KNKLTDFMHFYQMIDVLIIDDVQDFAGKPGTQNAYFNIFNHLHQSGKQLILTSDKAPV
                     DLKDLDDRLLSRFKWGLSAELQSPDYATRLAILEKKCYNEGVEVPREVLEFIASQVTT
                     NIREIEGTFCSLLAQSTFNKQPVTLKLAQGITDRLVNSMPREVSVQDIQKTVCNYFGI
                     THDVLLSKTRKREICQARQIAMYLSRNMTKNSLSTIGSMIGGKDHATVLHSYNTVCNL
                     MDTDRTFRQYVTDIEKSLKNG"
     gene            complement(16287..17198)
                     /locus_tag="PLM68_00805"
     CDS             complement(16287..17198)
                     /locus_tag="PLM68_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT53944.1"
                     /translation="MKIKKYSREFKIGVFSLVVIVALYFVIQFLKGNDFFRGTSTYYA
                     IYPNVAGLAPTSPVSVLGLTAGTIDKIDFDQEKQHMVVTMKLKKGFRLPLGTEARIYS
                     ADILGGKAVQLILGDSTGWHQPGDTLDVSIERDLISMLTTEIVSLKDDISTLIANLNQ
                     TVLQLNDILDSENSGNIRESLNATLGHVNSLAETLNRNSGQIDRIFMGVDTLATGLNK
                     AMADLSSTLDNFDDISAGLKDADLAGTIEGLHKLLDNLSNPEGTVSLLGSDPGLYNAI
                     TDIISRADSLIRLISDNPKKYLKITVF"
     gene            complement(17204..18304)
                     /locus_tag="PLM68_00810"
     CDS             complement(17204..18304)
                     /locus_tag="PLM68_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT53945.1"
                     /translation="MKKLIPVLVAVFTVCNSYAIMGQPVSGPVIRKVVIDPGHGGHDP
                     GALSPDRKLREKDINLSVARYLGQEINKNYPEIEVLFTRDKDFAVDLDKRGTIASEQH
                     ADLFISIHVNSARKNTSVSMGPETWVFGGDRSLRKDDSYDVVLKENEVIRFEDNYKTK
                     YEGFDPDNPQSMIVFNFQKDAIMRQSRILGTCVQQSLRQGPLRGVGNDRGMKEGGFVV
                     LAYCSMPAVLVELGFINSSTDRRVLSSEDGRRNLARQLFTGFEKYMETYAENGKLHMT
                     LDEEDAQTEKRYRVQVMASMEKLPDSDGVLKTYPQIQYIVSPDQIYKYKYTMGDFITI
                     EEAQQYCNTLRSGLFPDAFIIAVKDGKLVPVN"
     gene            18320..21088
                     /locus_tag="PLM68_00815"
     CDS             18320..21088
                     /locus_tag="PLM68_00815"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOT53946.1"
                     /translation="MSAEDKDNKSILHTICCLLFLMLGTTILQGQQKRFPENAIAPVS
                     ELYKKSLYLPVTARPEKTPLAVMWETLGKYTGMEKPEVGLSVADMDSLARLDSVRAHT
                     QLEAPAFSMARDSVVEDFRNGRTLLYYYGDVKVTYDNMEITSEYMEYNADTKTVFATG
                     IIDSTGVMQGKPVMKEGNNNYEMESVYYNFTSRKARINNMVTQDEQGFLHGTTLKKMP
                     DNSINIKGGKYTTCDAEHPHFYLHMTNAKITSDEDGNVGQTIFGPSYVVIEDVPTPIV
                     VPFGFVPKRPERSGGLLIPSYGEEVARGFFLRDLGYYFVFGDYLDFSVTGDIYSLGSW
                     SIKGTARYKNRYKYNGNLNVNYSKNVTGERGSADYQESGDFSIRWSHTQDPKKRPGTS
                     FSASVNFSSPTNNRFNSRSVDEALQSQTSSSISYAKTFAGTPFNLSINLLHSQNNRDS
                     SYALTLPNLTFTMSRINPFQRKERIGKKRFYEDISISYNTAFQNKLTFKASQFGKPEF
                     WDDYKTGMQHNFAIGLPSFTLLKYLNFTPTVSYGMNWHFMSETRTFDPARQKVVSEMS
                     KPFSEFGITQSYSGGISMSTRIYGMFNFKPTGKLRAIRHMMTPSLSFGYHPELGTRAN
                     GFVTLDYTDSLGREHSVEYNKYSRSVYGPPSKGRTAAISFSLGNNLEAKVFNKKDTTE
                     NGELKKVKLIDNLSLSGSYNFLADSMRLSNINVTLSTSIFEKLGINANAMFDPYAVNE
                     HGERINTFNVVKTGNLARLTNASFSTSYSFNGGGDKSKNDAYQLIYEDPLTGEYIPGG
                     WVYYMEPEIPWSVNLNYSFSYNKSYTYANEQLNVNHNFTQTLGFSAQVKLTKDFSINI
                     RSGLDLMKFRLTTTQLSATYDLHCFQISFSWVPNGQWESWSFRIAAKASALADLLQYT
                     KNSSYWDN"
     gene            21161..23071
                     /gene="rho"
                     /locus_tag="PLM68_00820"
     CDS             21161..23071
                     /gene="rho"
                     /locus_tag="PLM68_00820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT53947.1"
                     /translation="MYDIIELSSKSMDELHRIANTLDIKKVESYAKNELIYQILDVQA
                     ESGAASAASAPKTTNAKNRTSQRARKPKANAQPGEIFPPVQQAKESKQPVHDTNKAEQ
                     QPLKSSPMARPEKRDLPKQDVKATPGQEAKEVPSPRNIEPAKDPAREQARETAKEPAR
                     ETAKEPAREQAATLPLQEPAVEEIKADAAKTGSKDAKPVTKAKKGRPRKNEESDRTSE
                     HTVTKSNMVEPVAESAAAELAPVEHAPGHKHHQEHAPKTNAEKKPEYEGVVRAIGVLE
                     MMPDGYGFLRSSDYNYLNSPDDIYLSQQQIKMYGLKTGDTVSGEIRPPRESEKFFPLV
                     KIHEINGREPEFIRDRIPFDFLTPLFPDSKFDITSKGHNILSNRVVDLFTPIGKGQRG
                     LIVAQPKTGKTILLQTIANAIADNHPEVYMIVLLIDERPEEVTDMARNVKAEVIASTF
                     DESAERHVKVAGIVLEKAKRLVECGHDVVILLDSITRLARAYNTVQPASGKVLSGGVD
                     ANALQKPKRFFGAARNIENGGSLTILATALIDTGSKMDEVIFEEFKGTGNLELQLDRK
                     LSNKRIFPAVDVTLSSTRRDDLLLEKEKLNRIWILRNYLADMNSVEAMEFMKERLLRT
                     QDNDEFLATMSK"
     gene            complement(23055..24122)
                     /locus_tag="PLM68_00825"
     CDS             complement(23055..24122)
                     /locus_tag="PLM68_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOT53948.1"
                     /translation="MWFTEVIKQSVLITGLVMVMMILIEYINVSSKGTVLKQLQNKPM
                     LQILISALLGLIPGCSGGFVVVSLYAHNLVSFGALIAMMIASVGDEAFVMLAMIPETS
                     LLLFAILFVMAIVAGFLTDRFIHRIPGPFSPEHFVVHEHESGKHENLQIRGSIRDNFS
                     PLRWRRLVLILGLVLFSASIVFGLLEHDHDAHEHTQNLLFSERWLNLTFAGLSLLTFV
                     LTLFATNHFIEEHIWEHVIKHHFPKIFMWTFGTLVVIHLLMNNIDMHTWIREQFWLVL
                     PVAILVGIIPASGPHLIFVTLFASGVIPFSILLANSIVQDGHAGLPLLAETKKGFFTA
                     KALKIILALAVAVAWQLIYSW"
     gene            complement(24129..25190)
                     /gene="meaB"
                     /locus_tag="PLM68_00830"
     CDS             complement(24129..25190)
                     /gene="meaB"
                     /locus_tag="PLM68_00830"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT53949.1"
                     /translation="MDSALTVNQGVPQPPDINPRARDIIKKKPGKSLAVPVYLEGIRS
                     GDVPLLAQSITLIESTLEEDRKKARELVSACLPLSGNSVRIGITGVPGVGKSTFIEAF
                     GQYIISAGHKLAVLAIDPSSERTKGSILGDKTRMETLANNPRAFIRPSPSGGTLGGVA
                     RKTRETIILCEAAGYDTIFIETVGVGQSETAVHSMTDFFLLLMLAGAGDGLQGIKRGI
                     MEMSDLLVINKADGSNIDNARKARALYKGALELFPPPPSGWKPLALCASAIEGTGMKT
                     ILEAVEEYMRQVKENGYFQTRRAEQAVYWMRESIDDYLLSRFYDNPRIKGRLSEMERK
                     VREGETDSFSAASELLKHL"
BASE COUNT         6943 a         5931 c         6123 g         6359 t
ORIGIN      
        1 tttaacaaaa aaaatcaaga taagatatga cagtcaaaga aatggcagca gccttacaat
       61 tacgtgtcct tgccggagaa gaaggattgg aaaaacaaat tgctggcggt tacacctccg
      121 atttactgag cgatgtgatg ggatatgccg atgcaggcat ggtatggatc accttgcaaa
      181 cccacaagaa tatcatagcc atcgcctctc tcaaggaact ctcggccgtt gtgctggtaa
      241 aaggctttga acccgatgcc gacacggtgg cccttgccat tcaggaacag attaccttgc
      301 ttggaacaac cgaacaggcg tttgaatttt ccggacgtct gtacaggctg ctttatccct
      361 gattatgatt tgtgctaaag ccgatctgca tattcatact tttttatctc cctgcggaaa
      421 cattgggatg actcccctga acatagttgc gcaggcaaaa gccaaaggtc tgcaactgat
      481 cgctgtcacc gatcacaact caacgctcca ggggccagtg gtccgacagg tgtgtgaacg
      541 cgaagggata gctgttattt acggagccga gatcaccaca cgcgaggaag tccactgtct
      601 ggcttatgtg ggatcggaag agcagcgtgt ggaattgcag cattacctgg aaaagcacct
      661 gccgcatgtt cccaacaatc ccgacatctt cgggtatcag ttgtgggttg atgagcatga
      721 acaggtgctg ggagaagctc cttacttgct aatatcggcc atagatcagg gaattgagca
      781 ggttgccgcc tttgtgcatt ccattgaggg actttttgtt ccggctcaca tagaaagatc
      841 caggaattca ctgctaagcc aattgggttt tgttccgccc ggattgcccg ccgatgccct
      901 ggaactatcc cgttatacca atccggttga tttttgcagt aaaaatgcca ccctaaaaaa
      961 atatagaatc attcaatctt ccgatgccca ttttcccggt gacataggta cggcagccac
     1021 atcttttctt atggagaaaa cgggttttgc aggattgaaa acagcactca catcaaccca
     1081 tgaatgatct ggcactgcat ttgcttgata ttgtacagaa ttccctggtt gcaggagcct
     1141 ctttcataac tatagaggta aatgaaaatc cggcaggtaa cttattaact atcagtgtta
     1201 aagataacgg gcgcggtatg accccggaac aagtgcaaaa gctgagtgac ccctttttta
     1261 cctcacgtac tacccgcagg gtggggctcg gtgtcccgct gctcagacaa accgccgaac
     1321 aaagcggagg cagccttgag gtggtctcgg aaccgggaaa aggcaccctc ctgacagtct
     1381 gttttcaata cgaccacctg gacaggcctc ccctgggaga tgtggccaat gcagtggtgt
     1441 tgatgatctc ggcaaatccg gaagtgggat tttcatatca ttatgcatac aatgacaggt
     1501 cttatgaggt taacaccatg gaactgaaag aagtgttggg agatttaccc gttaacgatc
     1561 tgtatgttat ggaaatgatt gaggaaatga taaaggagaa tcaaagtgac cttaaagttt
     1621 gtcaaattga ataatttggg cttactttgc cgattccttt ttttactact gtataaaaca
     1681 ttattgagat tatgacaaag attaaatcat tggacgattt gcggcaaaga aagcagcagc
     1741 tcgaacaaag tatggaaatc agggaaaata gcgaccatcc cgatagcctt gtacaaatta
     1801 aggttgcgat ggccacatgt ggcatagcct caggggcaag ggctgtcatg aatgcctttg
     1861 tagagaagat aaaaaaagaa aagatacctg ctgtagtaac acaaacagga tgcatggggt
     1921 attgttatgc agagcccacc gttgaggtaa aaaaaccagg aatggatccg gttgtatttt
     1981 cctatgtcga tgttgccaaa gcagaagaaa tagttgacaa atatatacgc aataacgaat
     2041 tgcttgacgg tatcctgccg gcgaactatc agactattta aaccattcaa taatatataa
     2101 tcataatgta taaaatgcaa ttacttgttt gcggcggaac cgggtgccgg gcctctgcaa
     2161 gcgctaaaat tgtagaaaaa ctgaatgaat cactcgcttc acacggattg acccaggacg
     2221 cccaggtcgt tacaacgggc tgttttggtt tctgtgaaaa aggtcccatt gtgaaagtga
     2281 ttccggacaa caccttctac acacaagtca agccggaaga tgcggaagaa atcgttgccg
     2341 aacacatcct caaaggaagg aaggtaaaac gactgcttta cgaaaatccc gaaacagagg
     2401 aacatattcc cgattccaaa cacatgggat tttatcagaa acaattgcgt atagccctca
     2461 ggaactgcgg tttcattgac cctgaaaaca tcgatgaata cattgccagg gacggttatt
     2521 ctgctttggc acaggtagtt actgagctca ctccgcagca ggccattgac atggtcaagc
     2581 aaagcggatt acgcggaagg ggaggagcag gttttcccac cgggctcaaa tgggaaatca
     2641 caaagaattt cacgtcagac cagaaatacg tggtatgtaa tgccgacgag ggagacccgg
     2701 gagcattcat ggaccggtcc atcctggaag gcgaccccaa taccattatt gaagccatgg
     2761 ccatctgcgg ttattgcatt ggggcaagca aaggcctcgt atacattcgt gccgaatacc
     2821 cactggccat caaacgtctt cagatagcca tcaaacaagc aaaagaatat ggtttactgg
     2881 gtgacaatat attaggaacc aatttctcat ttgacataga gttgcgttat ggcgcaggtg
     2941 ctttcgtctg tggagaagaa acagccctta tagcttctat ggaagggaaa cgcggagaac
     3001 cgagggtcaa acctcctttc cctgcagaag acggatacca gcacaaaccc accaacgtga
     3061 acaacgtgga gacttatgcc aacgttcccg tcatcttcct caagggaccg gaatggtttt
     3121 ccagcattgg tacagccaag tccaaaggca ccaaggtttt tgcccttgcc ggcaagatca
     3181 ataacgtggg acttatcgag gttcccatgg ggatcacatt acgcgagatc atctatgaaa
     3241 tcggtggcgg tataaagaat gggaaaaaat ttaaggcagt ccagacaggc gggccttcgg
     3301 gcggctgtct gaccgaaaaa cacctggata cacccattga ctttgacagc ctggtggctg
     3361 ccggttccat gatgggatca ggagggatga tagttatgga cgaagatgac tgtatggttt
     3421 ccatagcaaa attctacctt gaatttattg ttgaagaatc ttgcggcaaa tgcaccccgt
     3481 gtcgtgtagg caacaaacgt atgctcgaaa tcctggaaaa gatctgccag ggcaagggta
     3541 acatggagga tctggatcac ctgcgtaact ttgccaacgt gattaaagac accgcattat
     3601 gcggactggg acagacctct cccaatcctg tgctttccac catggagaac ttctatgacg
     3661 agtacctagc acacgtgaag gataaaagat gtattgccaa aaaatgcaaa gcactggcca
     3721 cttatacgat catgcccgat gtatgcacgg gatgttccgt ttgtgccaaa aattgtcccg
     3781 ttgaagccat cacaggggaa aggcggcaac cgcacttcat caaccagaat aaatgcatcc
     3841 gctgcggggt atgctatacg aagtgtaagt ttaatgctat cttagttcaa taagcgagaa
     3901 tatggaaaca gtaaaattaa ctatagacaa taaacacgta gaagttccca agggaacgac
     3961 cattctccag gcggcaaaac aagcgggcat caatattcct tcgctttgtt atatgaacct
     4021 gcctcacctg gcccaggaac acaaaccggg aagctgccgc gtctgtgtgg tggaaatccc
     4081 tggaaagaaa acactggttc cggcctgtgt aaccaaatgt acagatggca tggaagtttt
     4141 gtctcactca ctgcgcgcca taaacgcccg caggaccgtg gtggagctgc tgctttccga
     4201 ccacccgaaa gattgcctta tttgcccttc ttccggtaat tgcgaattgc agaagctttc
     4261 catcaatatg ggaatccgcg agatcaactc ggtggaaaac gcagccatat ctacataccg
     4321 caaagactct tctccttccc tgcttcgcaa tatggataaa tgcgtcatgt gccgcagttg
     4381 tgaaaccatt tgcaacgaaa tccagacagt tggcgcactc agtgctgtga accgcggttt
     4441 catgtcggta gttgcccctg cctttgaaca ggacctgaca gattctccct gtaccttctg
     4501 cggtcagtgt gtggctgtat gtcccaccgg tgctcttaca gcagttgaca ataccttcca
     4561 cgtgatacgc gccctggccg atcctaccaa gaccgtactc attcaaacgg ctcctgcagt
     4621 acgtgcagct ttaggagaag aattcggcta cacacctgga accttggtta ccggtaaaat
     4681 ggtggccgct ctcagagccc tgggctttga caaggtattt gacacagact ttgctgccga
     4741 cctcaccatc atggaggaag gaacagaatt gctgaacagg atctcgcgtc atttgagcgg
     4801 agataaaagc gtgcgtctgc ccatactgac atcctgctgt ccggcctggg tcaacttctt
     4861 tgaaaccaac tattccgata tgttggatgt tccttcgaca gcacgttcac cacaacaaat
     4921 gttcggttcc attgcaaaaa cctacctggc agaaaaaatg gggataaaga gggagaacat
     4981 ggttgtggta tctgtgatgc cttgccttgc caaaaagtat gaatgccaga gggaagaatt
     5041 caaggttgac gggaatcccg atgtggacta ctcaataacc acccgcgagc tggctcattt
     5101 gatcaggatg ggcaacgtgg atttcaaatc cctcaaggat gaggattttg accgtcctat
     5161 gggagaatct accggtgccg gtgtcatctt tggtgctacc ggtggtgtaa ttgaagctgc
     5221 cacccgtacg gcatatgaac tcttcacagg caaaaaactg gaaaaagtcg atttttacga
     5281 actaagggga atggatggga tcagaaccgc tactatcgat tttgacggaa ccgacattca
     5341 tatcgggatc gcccatactc tgggcaatgc cagaaaattg ctggatggca tcaggaaagg
     5401 catgtacaat ttccacgcta tcgagatcat ggcctgcccc ggaggttgta tcggaggagg
     5461 aggacagccg ctccacaggg gcgactccat tgttctgaag gctcgtatgc aggcaatata
     5521 caaggaagac cttaacaagg taatccgcaa atcacacgag aatcctgaaa ttgccaggct
     5581 ttatgaggaa ttcctcggga aacccctgag tgagatgtct cacaagctgt tgcataccca
     5641 ttatttcagt cacccgaaaa aataaccatt aagttctatt cctatgatga agtttgaaat
     5701 aaatgattgt cacaaaaaga caataaaaga aatatgtgat tcttacggga acaagagaag
     5761 tgaactgatc aatatccttc acgataccca ggagcatttc ggttatttgc ccgagcctgt
     5821 acagctggaa atagccctga acctgaatat gccccttgcc aaagtttacg gtgtggtttc
     5881 tttctactca ttctttacaa tgactcccaa agggaaatat cccatttccg tttgcctggg
     5941 aacagcatgt tatgtgcgtg gtgccgaagg gattgtagaa gccattgaaa aagaactgaa
     6001 gatcaaagtg gggcaggtta cagaagacgg caagttctcc ctggattgcc tgcgctgcat
     6061 aggtgcctgc ggactcgccc cggtcatgat gataggtgat aaggtgcatg gacgccttga
     6121 acctgaaatg ataaaaaaca tcctgagtca gtacgagtaa ccggactttt ttttttagac
     6181 agggagtgtt ccgtccaggg acactccctg tttgttttcc tgaaaaaaag tatctttgcg
     6241 cgttaataat taagtatgcc tgaaaattca ttgttaaaca gactggaagg tatccgtcgg
     6301 aaattcgaat cattgcagca gcaattatca gattcctccc tgatacagga catgaagcgt
     6361 tatgttgccc tgaataagga atatgccggg ttagagccct tggtcagggc cggtgataaa
     6421 taccgcacag ttatccgcaa cctgtccagc gccaaagaac tcctgctgac agaaaaggat
     6481 gaggaattgc gtgaatttgc caaagaagag attgaccggc tggaagagga aataccggaa
     6541 atggagcaac atataaaatt tctgctcatt ccggctgatc cgcaggatag taaaaatgcc
     6601 atagtggaaa ttcgcgcggg gacaggagga gacgaagcct caatctttgc aggtgaccta
     6661 ttcaggatgt acagcaaatt tgcagaaaag aacggatgga aacttgatat tacaggtctt
     6721 tctgaaggaa catcgggagg ttacaaagag ataattttca agatatccgg gtctggtgtg
     6781 tacggaattc taaaatacga aagcggtgta cacagggtgc agcgtgtacc ccagacggaa
     6841 acgcagggaa gggtgcatac ttcggcagct tctgttgttg tattgccgga agccgatgaa
     6901 tttgacgtgg agctcaacga aaaagatatc cgcaaggaca cgttttgttc ttccggtccc
     6961 gggggacaga gcgtgaacac aacttactcg gccatacgcc ttactcacat tccaacggga
     7021 atcgtggtgc aatgccagga tgaaaaatca cagttgaaaa actatgaaaa agctctttct
     7081 gaattacgga cccggattta taacatggaa tatgagaagt atctgaccag tatcgcggcc
     7141 aagagaaaaa ccatggtctc tacaggagac cgctctgcca agatcaggac ttacaactat
     7201 ccccaaagcc gtgtgacaga ccacaggata aattatacga cgcataactt gcctatattc
     7261 atggacgggg aaatacacga agtcattgat ctgttgcagg tagcagagaa tacagaaaaa
     7321 ctgaaagatg aaccgtcaaa cacttaaccg gctgatactg gaaaaacgca gctgcctctg
     7381 cgttggattg gacatttctt ccatttcttt tgagccgatc aggcggattg ttgaggagac
     7441 cgctccatat tgcgttgctt tcaaagcaaa tacagctttt tttgaggctc acggctggaa
     7501 aggatggaag tgtatggaag aaacggtttc ctacatacgc tcccggtttc cggatcactt
     7561 cctgattgca gatgccaaaa gaggtgatat tgggaacacg gcagtgcaat acgcccggac
     7621 attttttgag agaatggatt ttgatgccgt gacactgtct ccttacatgg gttatgacgc
     7681 ggtggaaccc tttcttgcat accgggacaa atgggtcatc ctgcttgccc ttacatccaa
     7741 cacgtccgca gcagattttc agttttcctt gacaccggat ggcgccccgc ttttccagaa
     7801 agtcattgaa aaggccaata cctgggccgg cagagacaaa ctgatgtatg tggccggtgc
     7861 aacccggcct gacatgttgc gtctggtgag ggcaatggca cctgaccggt ttctgctcat
     7921 ccccggggta ggggcgcagg gagggacagt ggaagaggtg atgcagcatg caaaaacgcc
     7981 ccagggagat atcctgatca atgtttccag ggcaattgtt cagtctgagg atggtcccgg
     8041 gatcgcagcc aaagaatttg ccggatccat agcccggttc ttttcgtaat ccggttcatt
     8101 gacaagcagc ttttcataag acctgtcaat tttaccgcta tccccgtcat atagtgtttg
     8161 gtaccacttt tgtatgtgta ggagtacctt cggtaacaat tcatccacat tgtaccggat
     8221 acctgtttcc aatacaatgg atgtgggatt gggagctccc ccgaattgtt cttgattgac
     8281 attgatccca aacccgataa tactggaagt caggttgttc cccgaaagct gatgctcaat
     8341 gagaatcccg cagatcttgt tgggaccgat ataaatgtcg tttggccatt taatgcgtgg
     8401 agacaagcca agggtgacaa gaaaatcgca gatggccagc gctgtgcact ttgaaataag
     8461 gaattgtttg tttgcaggaa gaaaaacagg tcttagcagc agggtggcca gcaggttcag
     8521 gcccttgacg ctttcccatc tgcgggtata ttgtccgcgt ccgtttgtct ggaaagaggc
     8581 agtccataca gtaccttccc tggcatgttc caggttgcgg cgggcttccg tgttggtcga
     8641 atctatttca tcgaacctga tgatatgcaa gctaaacatg gtcgcaaaaa tactaattta
     8701 tccttacctt tgtaaggaac agaaagtaac gaatgaaaga agttgaaata attgccgggg
     8761 caatgcttga caaaaaagcg caagatgtat ccagcctgga tctgagaaaa ctgggtacaa
     8821 caatctgcga ttattttgtc atctgtaatg ccgattccgg tgtgcaggtt gctgcgatcg
     8881 cagacaatgt cgaagaacaa atgatcctga aagccggacg gaaggttaga agaagccagg
     8941 gaaaagaaaa ccgtttctgg gttatcctgg actacagcga tattgtagtt catattttcc
     9001 agacagagta caggcggttt taccacctgg aagatctctg ggccgatgcg gttaccaggc
     9061 actacactta atgaatagga tttaattatg aacggaaatt tccccaacaa gaacaaaccc
     9121 aataaatcac ccctgctgca gccaaaaagg cgctttaacc cttttctatg ggtttatgtc
     9181 atattgattg cgctgattgt ctatttcatg tttgccaaca ggtccggaga gcctgtgaaa
     9241 acagagtgga tcactgtcaa ggagcagatg ggacccgggg gtgaaattga gaaaattgtg
     9301 tatatcagca atatgcaacg tgccgagttg tatatcaaac cggatgcgct tgaaaaatac
     9361 aaggcaaaat tcggagacaa ggtacccaaa tttgctccac agttttattt tattgtcaca
     9421 gaaaattttg acgctgaaga acaattcgag gttttacgta attcacttca ggaagagcag
     9481 cgttttaaaa ttgagataga acagcgcacc agttactggg gacggatcat tgactggctt
     9541 ctctttcctg ctttgctgat agtgatgtgg atattcatgt tcaggcgaat gtccagggga
     9601 tccgaagggg gttccggtgg cggtggcata ttcaatgtag gccgttcaca ggcaaagctc
     9661 ttcgaaaaag acaaaaccgt tgccaatgtc acgttcaagg atgttgccgg cctggaagaa
     9721 gccaaggttg aaatcatgga gatagtggat tttctgagga accccggcaa gtataccaac
     9781 cttggtggca agatcccaaa aggcgcattg ctggttgggc ccccgggaac gggaaaaaca
     9841 ctgcttgcca aagctgtggc cggagaagcg aacgttcctt tcttttccat ctcgggctct
     9901 gatttcgttg aaatgtttgt cggagtgggg gcatccaggg tcagggacct gttcaggcag
     9961 gccaaagaga aagcaccctg cattgttttt atcgatgaaa ttgatgccgt gggcagggcc
    10021 cggagcaaga ataccggatt tacatcgaat gatgaaagag aaaacacact gaaccagctc
    10081 cttacagaaa tggacgggtt tgggtccaac agtggtgtga tcatactggc agccaccaac
    10141 cgcgccgatg tccttgataa ggccttgatg cgcgcgggac ggtttgaccg tcagatacat
    10201 gttgacctgc ccgagttaaa ggaacgtcat gaaattttca aggtccattc gcagggatta
    10261 agactggaag aagggtttga cctgggattc ctggcaaaac agacgcccgg tttttcaggc
    10321 gcagatatcg ccaatgtctg caacgaggca gccctgatag ctgcacgtaa aaacaagaaa
    10381 gaaatacaga agcaggattt cctggatgcc gtagaccgca ttgtgggcgg actggaaaga
    10441 aagagcaaga ttatcagtcc gcaggaaaaa cgcaccatag ctttccatga aagcggacat
    10501 gcgaccgtaa gctggatgtt gaagcacgca aacccgctgc tcaaggttac catcattcca
    10561 agaggccggg ctcttggggc ggcctggtac ctgcctgaag aacgccagat cacaacagtt
    10621 gagcaaatga tggatgaaat ggcttctgcc cttggagggc gggctgcaga ggagttgatg
    10681 ataggtcaaa tttccacagg agctatgaat gacctggaaa aggtaacccg ccaggcttat
    10741 gcgatggttg cctattttgg gatgagtgag aaaatcggga atgtttcttt ctatgattct
    10801 actgaaaatg ccggattcaa cctggggaaa ccctatagtg aaacgacggc gcagctcatc
    10861 gacaaagaag tcagacggat tatagacgaa gcttacgata cagcacgcaa ggttttgaaa
    10921 caaaataacg agggatttct taagctggct aaccagctgt tggataagga agtgattttt
    10981 gccgatgacc tggaagcgat tttcggtaaa cgtccatctg tagaaacgcc tcaggaagag
    11041 acaattaacg ctacggaaaa tagtgaacac gttaattaaa aggtctttaa gcggcctggt
    11101 attcttaatt ctgatgattg caggactact cctgcaccct tttggatatg ccatcctgat
    11161 gacagcctgc gtggggatca tggtgataga gtattacagg atgtccctgg gttcatggaa
    11221 caaggccgga cagatatttg gttttctttc cgccgtcatg atattcctgg gcacttatat
    11281 catgtcgcgt tacggagtct ggacagcacg ttacttcctc ctgttcctgc cactgcctgt
    11341 aacggtgact tggattaccg tattgttcaa caagtccgaa aatgtttttc aatatgcagc
    11401 ctatctcttt ttgccactgg tatacatagc cgttcctttt tcgaccttca attttctggt
    11461 attcaattca ctgggtatgt tccagggaat gtacctgctg gtactgttca tcctgctctg
    11521 ggtatctgat gtaggcggct accttattgg tatgggtttc gggcagaaaa acgggcacaa
    11581 actgtatccc aggatttccc cgaaaaaatc gtgggaagga ctagcaggaa gccttgtttt
    11641 ttccgtggcc accggactgc ttttatggaa gctggaaatg ttgccgttcc attggactca
    11701 ttgtctgata ttatcattga ttatcagtat attgggagtt gttggagatt tgacagaatc
    11761 ggcaatgaag agacatttcg gggtcaaaga cacaggccat atcatgccgg gccacggcgg
    11821 attgctggac cgttttgacg gagcccttat agcgatgccc attgccatag tgtatgtgtt
    11881 gtttgtcata ttcaaaggtt gatatatgat gaggatccat aaagaaggcc aaagcaccat
    11941 actggtagta atgtctgttg tgaccatttt tcttgtggtg atccttgctt tttcaccata
    12001 caatatctgg gtgcttgctt cattcatcct gttgtcggtg ttgttgctgg tccagaccat
    12061 gatgtttttc cggataccca gtcgcaacat cctgataagg gatgatggta ttgtctctcc
    12121 tgccgatggc aggattgtaa tgatccagga ggtgcaggtg aatgaatatc ttcaggaaaa
    12181 aaggttacag gtttcaattt tcatgaacct cttcaacgta cacgtgaact ggtatccctg
    12241 cggcggggac attgtctact ataaatacca tccaggagat aagatggttg cctggcatcc
    12301 aaaatcatca gacaagaacg agcacacaac attgtttgta aaacaggaca acacacttat
    12361 tggagtcaga cagatagccg gattgatggc ccgtcgtgta gtgtgcaaag ccaggaccgg
    12421 aaaacaggtg ctgcagtgca gcgaaatggg gatcatcaag ttcgggtcac gggtggatgt
    12481 cttgctgcca ccggatgcag aagtaaaggt cagaaaggga cagagggtat ggggatgcga
    12541 aactttgatc gccacgctta agagatagcg gtgatagcag ccagaggatc cggggatttg
    12601 aaaacataat ttcctgcaac aagaatatct gcgccgtttt cgtatagtat acgggcatta
    12661 gaatcgttga tccctccatc cacctgtatc aggaaatcca gtcctttctc ttttctgatc
    12721 ctgtcgaggc ggtttatttt ttctaaggat tcctcaatga aagcctgacc tccaaagccg
    12781 ggattgacag acattaccag cacaaggtca gctgaataaa cggcttgttc caggactgaa
    12841 acaggtgtag ccggattcaa ggcgatgcct gctttcatcc ccagacggcg gatctcctgc
    12901 agatcccggt tcaggtgagg acaagtttca tagtgaactg tcagccattc tacgccaagc
    12961 tccctgtagc gctcataata gcgggaaggt tccactgtca tcaggtgggc atccatgggc
    13021 tttcttgcat atcgcgcaat agcctggata acgggaaaac cataggatat attagggaca
    13081 aaaacgccgt ccatcacgtc cagatggata ctccaggcaa tgctttggtt caccatacgg
    13141 caggaatccg ccaggtgtcc gaaatcagct gaaagcattg ataccgctac ttttcccata
    13201 tcaaaccatc ttaagtatct tgagcagcaa agcccagaat tttgccgtac tttctatgtc
    13261 aagcctttcg gccggagaat gcactccttt cagggtagga ccaatagaga tcatatccag
    13321 gtaaggatac gcatccagga aaatgccgca ttcaagcccg gcgtggattg ctttgacttt
    13381 taccggggtg ttgaacagct cctggtaagc ctgaacacat atcttcagtg tgggagaatc
    13441 aggattgggt gcccaccccg ggtattcacc ctggtgtgtg acttctgcac cggccagcaa
    13501 aaagaggctt tcaatccggg acgccgcgtt tcttcttgcc gagttgatgg cactccgctg
    13561 gctgcttcca atgcggatga gatggttgtc cggctccatt ttcaccgaag ccagattggt
    13621 tgatgtctcc accagaccgg gaacatcctt gctcatggca agcactccat gggggcagga
    13681 atacagtgca gatacaaggt tccgggctgt cttcctttca attaccgcat ccggaaactg
    13741 gcaggaaagg atctggctct gaagttcagg atccgtatgc ttgtattctt tttcccaatc
    13801 cgcggccata tgactgaata attccctgaa agaggcttcg tcttcttttc ttataacaca
    13861 aacggcactg gcttccctgg caattgcatt acgtttgtta ccgccggaga gtgagcttat
    13921 gtcaacacta aaatgttgct gtgctttcca gagaaaacgg gcaagcacct gcactgcgtt
    13981 gcacaggtcc ttgtggatct cgtctccgct gtgtcctccc gtccctcctg atatgctcac
    14041 cgagagggca gtaagaccag taggcaaggt gggtatggaa tggtatgaaa aacaggctat
    14101 tgtgtctatt cctcctgcac aaccaatgaa cacttctcct tcatcttctg aatccaggtt
    14161 cagcaggata gtacccttga taaaaccggg ggcaacagcg cgagcaccgt caagaccgat
    14221 ttcttctgaa actgtaaata gacattccag cggtccgtgt tccagcgaat cgtcatccag
    14281 tacagcaagc gaggccgcaa cgccaatacc gtcatctgcc cccagggtgg ttccccttgc
    14341 cctgagccag ccatcctcta tgtaatactg tatggcatct ttggaaaaat caaacacagt
    14401 atcggcattt ttttcgcaaa ccatatcgat gtggctctgc aaaaccgcac aggggctgtt
    14461 ttctttcccc gccgaggcgg ctttacggat aacaacattg cctgtcttat ctgtctggta
    14521 atccagtttc ctgtctttgg cgaattgttc caggtaccgg gtcacaccgg attcattgcc
    14581 actttcgcga gggatcctgc agagttcaaa aaaaaagcca aatacggaat agggttccgg
    14641 agtgttcatc acaaagaggt ttaaccgttc ttgagactct tctcaatgtc ggtcacgtat
    14701 tggcggaatg tccggtctgt gtccatcaaa ttgcacacag tattgtatga gtgaagtacg
    14761 gtagcatgat ctttaccgcc aatcatggat cctatggtag agagagagtt ctttgtcata
    14821 ttccggctca ggtacatggc gatttgccgg gcctggcata tttcccgttt tcttgtcttt
    14881 gacagaagaa cgtcatgagt aatgccaaaa taattgcata ctgtcttctg aatatcctgt
    14941 actgacactt ctctgggcat ggaattgacc agtctgtctg tgattccctg cgccagcttg
    15001 agcgtgacgg gctgtttatt gaaggttgac tgtgccagca acgagcagaa tgtaccctct
    15061 atctcacgga tattagtggt aacctggctg gcaatgaact caagaacctc ccttgggact
    15121 tccacaccct cgttgtaaca tttcttttca aggatggcca gtcgtgtggc atagtccgga
    15181 gattgcaatt cggcagaaag cccccatttg aatcttgaca acagcctgtc gtccaaatcc
    15241 ttgagatcta ccggtgcttt gtcgcttgtc aggatcagtt gttttccgga ttgatgcaaa
    15301 tggttgaaaa tgttgaagta tgcattctgg gttcccggtt ttcccgcaaa atcctgcacg
    15361 tcgtcaatga tcagcacgtc gatcatttga taaaagtgca tgaaatcggt cagcttgttt
    15421 ttcacattca ctgcatccat gaactggatc tggaagaggt tggccgacac gtacaagacg
    15481 attttttcag gcatcctttt ctttatctca atccctatgg cttgtccaag gtgtgtcttg
    15541 cccagaccgg atcccccgta tacgaataaa ggattgaaag gtcctccggg tttgtcggct
    15601 atgcttaacc cggcagtacg ggccaggcgg ttgcaggatc cttccacaaa attatcgaag
    15661 gaataggcag ggttcagttg gggatcgatc ttgacctggc gtataccggg tatgacatag
    15721 ggactggcaa cttcatccct gtttatcctg gaaaaaggaa tggccttgtt ctggaattgt
    15781 gtctgtccct gttgtggcag aggcataccc ttgctgtgtg agtccacttt gacgcagtac
    15841 tcaagttttg ccatgcttcc caactctttt cgcagggaat gacccagaag gtcaatgaaa
    15901 tgctcttcaa tgtactcccg gaaaaaatga gaaggcacct caattgtcaa aacattgttc
    15961 tttagttgta acggtatgat gggttcaaac catgtgttat aacttgaagg ttgaatatta
    16021 tccctgatga tattcagaca attattccat acaatcttgt gaggggacgg ggccatattc
    16081 aaatttgggg gatttggtat acaaaaatgg ttgaaaaaaa tggtaaaaaa aaatgaaaat
    16141 cgtttggatt tttcaaaaaa acaataatgc tttaataatc aagaagaaaa aatgcaaaca
    16201 ttttaaaaca ctgataatga gatatgtaaa aaagcccgat ttttgctttc tattgacaca
    16261 tcagcatttg ggacaaaagt gaaaagttaa aataccgtta ttttcaggta tttttttgga
    16321 ttatcactga tcagacgaat cagggagtct gccctggaga taatatcggt tatagcgttg
    16381 tagagtccag gatccgagcc cagcagggaa acggtaccct ccggattgct cagattatcc
    16441 agtaatttat gaaggccttc tatcgtacct gcaagatctg catccttcag tccggcagat
    16501 atatcatcaa aattatccag tgtggaactc aagtctgcca tggccttgtt cagccctgtg
    16561 gcaagtgtgt ccactcccat gaagatcctg tctatttggc cggagttacg gttaagtgtc
    16621 tctgccagcg aattaacatg ccccagtgtt gcgttcagac tttcccttat gttcccgctg
    16681 ttttcgctat ccagtatatc gttcagctgt agtaccgttt gattcagatt cgctatgaga
    16741 gttgatatgt cgtctttgag tgatacgatt tcggtggtga gcatgcttat aagatcacgt
    16801 tctattgata catccagggt atcacccggt tggtgccatc cggttgaatc gcccaaaatg
    16861 agttgcacag ccttacctcc caggatatcg gcgctgtaga tcctggcttc tgttcccagt
    16921 ggcaggcgga atcccttttt tagcttcatt gtgactacca tatgctgttt ttcctggtca
    16981 aagtcaatct tgtctatggt tcctgccgta agccccagca cagagacggg gcttgtgggt
    17041 gccaggccgg cgacatttgg gtaaatggca taatatgtac tggtcccacg gaaaaagtca
    17101 ttgcctttaa ggaactgaat gacaaaatac aaggcgacaa tgacaacaag ggaaaaaaca
    17161 ccgatcttga attcgcggga gtactttttg attttcatat gatttagtta acaggtacca
    17221 acttgccatc tttaacggca ataataaagg catcgggaaa aagacctgat cgcagcgtat
    17281 tgcaatattg ctgtgcttcc tcaatggtga tgaaatcacc catcgtatac ttatacttat
    17341 atatctggtc gggagaaacg atatactgga tttgcggata tgttttcaaa acgccgtctg
    17401 aatccgggag tttttccatc gatgccatga cctgaacccg gtagcgtttt tctgtctgcg
    17461 cgtcttcttc atccagtgtc atgtggagtt taccgttctc ggcatatgtt tccatatatt
    17521 tctcaaaacc tgtgaagagt tgacgggcaa ggtttctcct cccgtcttcc gagctgagca
    17581 ccctccggtc tgtgctgctg ttgataaaac ccagctcaac caggacagcc ggcatagaac
    17641 agtatgccaa taccacaaac cccccttctt tcatacccct gtcgtttcca acacccctca
    17701 ggggaccctg tcgcagggat tgctgaacgc acgtgcccag gatcctggat tgcctcatga
    17761 tggcgtcttt ctgaaagttg aatacgatca tggattgtgg gttatcaggg tcaaaaccct
    17821 cgtacttggt tttataattg tcttcaaacc ggatcacttc attttctttc agtaccacat
    17881 catagctgtc gtcttttctg aggcttctgt cccctccaaa aacccacgtt tctgggccca
    17941 ttgacacaga agtgtttttc ctggcagagt ttacatgaat ggatatgaac aggtctgcat
    18001 gttgttccga ggcaatggtc ccccttttat ccaggtccac cgcaaaatct ttgtcacggg
    18061 tgaacaagac ctcaatttcc ggataattct tgttgatttc ctggcccaga tacctggcaa
    18121 cagacaggtt gatgtctttt tccctgagtt tcctgtcagg gctgagggcg ccaggatcat
    18181 gaccgccatg gcccggatct atgaccactt tacgtatcac aggccccgaa acaggctgtc
    18241 ccatgatggc atacgaattg cagacagtga aaacggcaac cagaacagga atcagttttt
    18301 tcatatatat ttgcacctta tgtctgcgga agacaaagat aataaaagta ttttgcatac
    18361 catatgttgt ctgttgttcc ttatgcttgg aacgaccatc ttacaggggc agcagaaacg
    18421 gtttcccgaa aatgcaatag ctccggtaag tgaactatac aaaaaaagcc tgtaccttcc
    18481 cgtaacagca agacctgaaa aaacacccct ggctgttatg tgggagactc ttggcaaata
    18541 taccggcatg gaaaaacccg aggtagggtt atctgtggca gacatggaca gccttgcccg
    18601 cctggacagc gttcgtgcac atactcagct ggaagccccg gctttttcga tggcaaggga
    18661 ttccgtagtc gaagattttc gtaacggcag gaccctgttg tattattatg gagatgtaaa
    18721 ggtcacgtac gacaatatgg agatcacctc cgaatatatg gaatacaacg cagatacaaa
    18781 aactgttttt gcaaccggga taatcgattc tacaggtgtc atgcagggaa aacctgtcat
    18841 gaaggaaggg aacaacaatt acgagatgga atctgtctac tacaacttca cctcacgtaa
    18901 agcccgcata aacaatatgg taacccagga tgaacaggga ttcctccatg gtacaacctt
    18961 gaaaaaaatg ccggacaact ccattaatat aaagggaggg aaatacacca cctgtgatgc
    19021 ggagcatccc catttttacc ttcacatgac caatgcgaaa atcacgtccg atgaagacgg
    19081 gaatgtgggg cagaccatct tcggcccttc ctatgttgtc atcgaagatg tcccgacacc
    19141 cattgttgtt cccttcggat ttgttcccaa acgacctgaa cggtccggcg ggttgctcat
    19201 cccatcctat ggagaagaag ttgcccgcgg ctttttcctc agggacctgg gctattattt
    19261 cgtatttggg gactatctcg acttttcagt tacaggggat atatattctc tggggtcatg
    19321 gagcataaag gggactgcac gttataagaa ccgctataaa tacaacggca acctgaatgt
    19381 gaattattcc aaaaacgtga cgggcgaacg cgggtcagcc gattaccagg aatcgggaga
    19441 cttctcaatc cgctggagcc acacacagga ccccaaaaaa cgacccggca catccttcag
    19501 tgcctcggtg aatttttcca gccccacaaa caaccgcttc aacagcaggt cagtagacga
    19561 agcattgcaa tcccagactt catccagcat ttcttatgca aagacgtttg ctggtacccc
    19621 tttcaatctt tccatcaacc ttttgcacag tcagaacaac cgcgactcat cctatgccct
    19681 gaccctgccc aacctgacct ttaccatgag ccgtatcaat ccttttcagc gtaaggaacg
    19741 gatagggaaa aaacgttttt atgaggatat cagcatcagt tataacacag cttttcaaaa
    19801 taagctgact ttcaaagctt ctcaattcgg caagcctgaa ttctgggatg attacaagac
    19861 ggggatgcag cataactttg ccattggatt accaagcttc acactgttga aatacctgaa
    19921 tttcactcca actgtaagtt atggcatgaa ctggcatttc atgagcgaaa ccagaacttt
    19981 tgatcctgcc cggcaaaagg tcgtttcaga aatgtcaaaa cctttcagtg aattcgggat
    20041 cacccaatct tattcaggag gcatttccat gtcaaccagg atatacggaa tgttcaattt
    20101 caaaccaacc gggaaactgc gtgccatcag gcatatgatg accccttccc taagcttcgg
    20161 ctatcacccg gaattgggca ccagggcaaa cggtttcgtg acactggact atacagacag
    20221 cctgggcagg gagcattccg tggaatacaa caaatacagc agatctgttt atgggcctcc
    20281 ctccaaagga cgtacggcgg ccataagctt ttccctgggg aacaacctgg aggcaaaagt
    20341 tttcaataag aaggacacca ctgaaaacgg ggaactgaag aaagtcaaat tgatagacaa
    20401 cctgtccctg agcggctcct ataacttcct tgccgactcc atgaggctct ccaacatcaa
    20461 cgtcaccctg tccacctcaa tttttgaaaa actgggcatc aatgccaacg ccatgttcga
    20521 cccctatgcc gtaaatgagc atggagagcg catcaatacc tttaatgtcg tgaaaacagg
    20581 aaacctggcc agattgacca acgccagttt ttccacttcc tattctttca acgggggggg
    20641 cgataaatca aagaacgatg cctatcagct gatatatgaa gaccctctga ctggagaata
    20701 cataccggga ggatgggtat attacatgga acccgagata ccatggtctg tcaacctgaa
    20761 ttacagtttt tcctataata agtcttatac atatgctaac gagcaactga atgtaaacca
    20821 taatttcacc cagaccctgg gattttcagc acaggttaaa ctgaccaagg atttcagcat
    20881 caacatcaga agcgggctgg atttgatgaa attcaggctg acaaccacac agctaagtgc
    20941 cacctatgac ctgcattgct ttcagatatc attttcctgg gttcccaacg gacaatggga
    21001 gagctggagc ttccgcatag cagccaaggc ttctgccttg gcggatttac ttcaatacac
    21061 gaagaattcc agttactggg ataattaaaa attttgttta tatttgcatc gtctttgctt
    21121 catctcgcag cttaattcat attttaacat attttcttta atgtacgaca taatcgagct
    21181 tagcagcaag tctatggatg agcttcatag aattgcaaac acattggata tcaagaaagt
    21241 ggaatcatat gcaaaaaacg aactgattta ccagatactt gatgttcagg ccgagtcggg
    21301 tgctgcttca gcagcttcag ctccgaaaac caccaacgca aaaaacagga cttcccaacg
    21361 tgcccgaaaa ccaaaagcaa atgcgcagcc tggtgaaata ttcccgcctg tgcagcaggc
    21421 gaaggaaagc aaacagcctg tccatgatac aaataaagca gaacagcaac ctctaaagtc
    21481 ctcacccatg gccaggccag agaaaaggga tctcccaaaa caggatgtca aggcaacacc
    21541 cggacaggaa gccaaagaag tacccagccc ccggaatatc gaacctgcaa aggatcctgc
    21601 aagagaacag gcaagagaaa ccgcaaaaga acctgcaaga gaaaccgcaa aagaacctgc
    21661 aagagaacag gcagcaacgc tgccgttaca ggaaccagca gtggaagaga ttaaggcaga
    21721 tgcggcaaaa acagggtcaa aagatgcaaa acctgttaca aaagcaaaaa aaggcagacc
    21781 cagaaaaaac gaggaatctg atcgcacgtc ggaacatact gtcacaaaat ccaatatggt
    21841 cgagcctgtc gcagagtctg ctgccgcaga gcttgctcct gtcgaacacg cacccggcca
    21901 caaacatcat caggaacatg cccccaaaac gaatgcggaa aagaaaccag aatacgaggg
    21961 agtggtcagg gcgattggag tgctggagat gatgcccgat ggatatggat tcctccggtc
    22021 ctccgattac aactacctga attccccaga tgatatttac ctgtcccagc aacagataaa
    22081 aatgtacggt ctgaaaaccg gcgataccgt tagcggcgag ataaggcctc ccagggaaag
    22141 tgaaaagttt ttcccactgg tcaaaattca cgagatcaac ggacgtgaac cggaattcat
    22201 ccgcgacagg ataccctttg atttcctcac cccacttttc ccggattcaa aattcgatat
    22261 tacttccaaa ggccacaata ttttgtcaaa ccgtgttgta gacctgttta cccctattgg
    22321 caaaggtcag cgcggactga tagtggccca gcccaagaca ggaaaaacca ttctgcttca
    22381 aaccatagcc aatgccatag ccgacaacca tccggaagtt tacatgattg tgctgctgat
    22441 agacgaaagg cctgaggaag tgacagatat ggcccgcaat gttaaagccg aagtcatcgc
    22501 ttccacattc gatgaatctg ctgaacgtca tgtcaaggtg gcaggcattg tcctggaaaa
    22561 agccaaacgc ctggtggaat gcggtcacga tgtggtaatc ctgctggata gcataacccg
    22621 cctggcacgt gcttacaaca ccgtccagcc agccagtgga aaggttttga gtggtggtgt
    22681 ggatgccaat gccctgcaga aacccaaacg tttctttggt gcagcccgca atattgaaaa
    22741 tggaggctca ctgaccatcc tggccactgc cctgatcgat accggttcaa aaatggacga
    22801 agtcatcttt gaggaattca agggaacagg taacctggaa ttgcagcttg accggaaact
    22861 atccaacaag agaatattcc ctgccgtgga tgtcaccctg agttcgaccc gtcgtgatga
    22921 cctgctgctg gagaaagaga aactgaaccg gatatggatc ctgcgcaatt acctggctga
    22981 catgaactcg gtcgaagcca tggagttcat gaaggaaagg ctgttgcgga cacaggacaa
    23041 cgacgagttc ctcgctacca tgagtaaatg agttgccagg ccactgcaac ggccagcgca
    23101 agtatgatct taagggcttt tgctgtgaaa aagccctttt ttgtttctgc cagcagggga
    23161 agtccggcgt gtccatcctg tacaatgctg ttggccaaga ggatgctgaa cgggatgacc
    23221 cccgaggcaa atagtgtgac aaagatcagg tgaggacccg aggctggaat gatacccacc
    23281 agtattgcca ccggaagtac cagccagaac tgttccctga tccatgtatg catgtctatg
    23341 ttgttcatca gaagatggat caccaccagg gttccgaaag tccacatgaa gattttcgga
    23401 aaatggtgtt tgatgacatg ttcccagata tgctcttcta tgaaatgatt ggtggcaaac
    23461 aaagtgagga caaaggttaa cagagacagg cctgcaaatg tcaggttgag ccatctttcc
    23521 gaaaagagca ggttttgtgt atgttcatgc gcatcatggt cgtgttccag caaaccaaag
    23581 acaatgcttg cagaaaacag tacaaggcca agtatcagca caagccgccg ccatcgcagc
    23641 ggactgaaat tatcacggat acttccccgg atttgaaggt tttcgtgctt tccggattca
    23701 tgttcatgga ccacaaaatg ctcagggctg aacgggcccg ggatcctgtg tatgaagcgg
    23761 tctgtgagaa atcctgccac tatggccatg acaaaaagga tagcaaaaag aagcagcgat
    23821 gtttccggga tcatggccag catcacaaac gcctcatcac ccaccgaggc aatcatcatg
    23881 gcgatcaaag ccccaaagga taccaggttg tgagcataca ggctgacaac cacaaacccg
    23941 ccgctgcaac ccggaatcag tcccaggagg gcagagatga ggatttgcag catgggtttg
    24001 ttttgcagct gtttgagtac agtgcctttg ctggatacgt ttatgtattc tatcaggatc
    24061 atcattacca tgaccagacc ggtaatgaga acactttgct tgatcacctc cgtaaaccac
    24121 atgacatatt acaggtgttt cagaagttca gatgcggcac tgaacgagtc agtctcgcct
    24181 tccctcactt ttctttccat ttcggataat cttcccttta tccggggatt gtcataaaaa
    24241 cgtgacaaca aataatcgtc aatgctttcc ctcatccagt atacggcttg ttctgccctc
    24301 ctggtttgaa aatagccgtt ctcttttacc tggcgcatat actcttccac agcttccaga
    24361 atggttttca tcccggttcc ctctatggca gaggcacaca gagccaaggg tttccatccc
    24421 gaagggggag gagggaacag ctccagggct cccttgtaca gcgctctggc cttccgggca
    24481 ttgtcaatgt tgctcccatc ggccttgttg ataaccagca gatcggacat ttccataatg
    24541 ccccgtttga tcccctgcaa accgtccccg gctcctgcca gcatcagcag cagaaaaaaa
    24601 tcggtcattg aatgaacagc tgtctcggat tgtcccacac ccaccgtttc tatgaaaatg
    24661 gtatcatatc ctgcagcttc gcataaaatg atggtttccc ttgttttgcg ggcaacccct
    24721 cccagggtac ccccgctcgg cgaaggcctt atgaaagccc ttggattgtt ggccagtgtt
    24781 tccatgcggg tcttgtcacc caggatgctg cccttggtcc tttcactgct tggatctatt
    24841 gccagaacag ccagtttgtg acctgccgag atgatatatt gtccaaaggc ttcaataaaa
    24901 gtgcttttcc ccacccctgg cacacctgtt atgccaatcc tgaccgagtt tcccgataaa
    24961 ggcaggcagg ccgagaccaa ttcccgggct ttcttcctgt cttcctccag ggtgctttca
    25021 atcagggtta tggattgtgc cagcaggggc acgtcgccac tccttatgcc ttccaggtaa
    25081 acaggtacag ccaggctttt gcccggtttt ttcttaatga tgtcccgggc ccttggattg
    25141 atgtcgggcg gttgcggtac tccctggttt actgttaagg ctgaatccat acctttcagg
    25201 gtttgatttt gccatatacc aggagattca ccgtgggtct tgacggagca ttggtcgtca
    25261 ggctgatggt gtagatgact tctccgggtg ttcccgtttc cgtacccggg atgaggatac
    25321 ttaccgggac agttgctccg ggagcgattt gccggt
//
LOCUS       DAOSOF010000007        25024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_1996_length_25024_cov_6.133405, whole genome shotgun sequence.
ACCESSION   DAOSOF010000007 DAOSOF010000000
VERSION     DAOSOF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1996_length_25024_cov_6.133405"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLM68_00835"
     CDS             <1..519
                     /locus_tag="PLM68_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT53950.1"
                     /translation="RAILDGQTALYRLLVERYQNPVFRVIYKIVNNHEEAGELTQDVF
                     VKAYESLPKYDPRYKFFSWIYRMAINRALLCLRTRKPCVEADKIPAHERLAQKEEPFD
                     LVTKSTLIDKAVNELDKKYKTVIMLKYFAGLSYSEISETLDIPEKTIKSRLFDARMLL
                     KDKLEKKLKYVD"
     gene            509..1222
                     /locus_tag="PLM68_00840"
     CDS             509..1222
                     /locus_tag="PLM68_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53951.1"
                     /translation="MLTERDILLIQNKVDGMLSHREEDQFAALLEASPQAVDLYRKLL
                     AVHEEMYSSAARIPEIDITKQVMRRIEGKTFLHKLFTGSGRVYAFAAALVLFFVLGML
                     AATYLVPSLRNLSQEELAGTMMRKKTSLWSFHDTGIDIEVQQQQEGDLVIYRASVFTA
                     DSLNVEFSSPGEPVNVNLLKIIDFDGLAQKMENTDHKISYICQGNTVFTLTDPPDRSS
                     LTFSLEGKQAFHMKFPPTF"
     gene            1268..2383
                     /locus_tag="PLM68_00845"
     CDS             1268..2383
                     /locus_tag="PLM68_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53952.1"
                     /translation="MKTKNKSSIVLVLAVLVSLAAGFLVGILVDIPKTDNTQLAGTIG
                     RVQNYKNVKVTEEDIELKNDLVSDTLILNAISTYFNYHYVAAVSQGEKIRYALDALDN
                     QDAYREYAGLLLQEVSGYGTFLENARLDLLQAIAVVTNPAEVHPVMMRNTIVQANNVI
                     SQISYRKQSVLNLIDNLGSYLEKQDKEADATLASVHAVLSLEQVTNAMALNDKMVLKY
                     FEKKKIFTEQIQGSFGSELQGLIVEDAQRLGIDLNNIDPMGIIILNAESIGSHFALNN
                     IEVGSMINNMNELGVFGSEAVRSSNDLGSVAFMNLENLSLQIFGSSENLEAVLNAPDL
                     GMFNNMQELGFLNVNQLNVVMRVSELGGVIGIGPIGY"
     gene            2394..5342
                     /locus_tag="PLM68_00850"
     CDS             2394..5342
                     /locus_tag="PLM68_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53953.1"
                     /translation="MKKFKAIILLMVVIIPGPPGILRAQLINSVKKDNLHLVYFGNRY
                     SYIVPHVRQTYLNAMDFHKRFWSYRDTTTYVFLTDFEDTGHGGAIAMPYNQVILGIEP
                     YSLAFSIIPSNERFQWLFNHELTHIVLSEKPNTRDAKFRKFLQGKVIRADEYPISALW
                     SYFTTPRWYAPRWYHEGIACFMETWMSGGFGRAMGFYDEMYFRSIIEGKEDLYSLVGL
                     DTEGTTEDFQVGANSYLYGTRFVTYLAYQYGIDKLKDFYIRSDSSRAFYAGQFRKIYG
                     RPVREVWQEWMEWEQDYQQKTIARIGEFPLTTLRSLTEEPLGSVSRPEFNPETGKIYA
                     AINYPGIISQIAEIDRTTGSIRKVATLDSPKLYYTTHLAYNAEDEKIYTSEHNSNYRN
                     LVEIDVKTGSKKVLNKLTRAGDLVFNPADKSLWGIQNDNGYSILVKMPPPYDKALPVF
                     TVPFGRILLDPDISNSGKLLCATLSGIRGEQSLVLFDLEALDAGKSSMETIMELEDNT
                     LTQFRFSADDRFLIGTSYYTGVSNVWRIGLEDRNMELLSNSATGLFAPIQVTEDSLLV
                     LEYERDGMRPGIMPVTVLEDANAISFLGNMVHQAHPEVEEWSLPPASALEGVNDSIIE
                     TPYRPLKEMKIAGAYPDIAGFKNTVALGYRLNWRDPVGISNINLFLAVSPWSSYGIEQ
                     KFHAMLEWKFWSWRLKASYNPTHFYDIFGPTKRSRAGYSIGLGYQRTFILKDPLKYHV
                     DASVFTYGMLEVLPVYQEIASPIRNMQAASVTFGVSKLRKTLGGVDDEMGFTWDINAT
                     TYLAQKRFYPSFVSDQSVGFLIPFIRNTSFWIRNSVGISLGDRESSLSYFYFGGFGNN
                     YVDWQPSEQYRNTLSFPGARINQLPAYTYVRTMGELNLMPIRLRNVGTTWLYPTYIKP
                     SLYGAHLMTGFDRPQQTAHLFNCGGQIDIQLVLFSYLKTTWSFGYAKMFRAGEKSTNQ
                     FMLSLKLLGN"
     gene            5349..6926
                     /locus_tag="PLM68_00855"
     CDS             5349..6926
                     /locus_tag="PLM68_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOT53954.1"
                     /translation="MKNKFPAPLVIVFVLIVVAAMLTWFLPGGQYVEGTFQPADSRPQ
                     TWQVFTAFYNGFVRQAGIIVFILCVGAAFWVINATRAIDSGIRSFLDSAARLERFPLL
                     RRIGVNNLVIASVMVLFSLFGAVFGMSEETIAFTALVVPLALSMGYDSITGVCMVYVA
                     AHVGFAGAFMNPFTVGIAQDLSDLPIFSGMGYRLVCWALLTAGTIVFVLRYAHKVKRD
                     PKASPIWEEDASRKVAHEAGVDLLAEYPAEGAKTTWTAWMAFFLVLAALTAFSVSSPS
                     TMIALGAWKLKIPGLLPALTFLYAAGGLKILTGKDKQENPARLVRHFVLFAVLFLLVG
                     VLGYNWYINEIAGLFLALGILSGMAAGYRANTIAQQLTEGARDILGAALIVGLAAGIV
                     EILEQGRIMDRILFAMAGGLESVGRTGSLQVMYLIQTAINMLIPSASAKAAMTMPIMA
                     PFSDLVGVSRQATVLAFQFGDGFTNMITPISGVLMAVLGVARIPYTRWARWVWRFILG
                     LIVTGALLLLPTLFIWK"
     gene            complement(6975..7844)
                     /locus_tag="PLM68_00860"
     CDS             complement(6975..7844)
                     /locus_tag="PLM68_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOT53955.1"
                     /translation="MRVSESMTSKGCIFDIRRYAIHDGPGIRLNVFFKGCPLHCQWCH
                     NPEGQDPEPVVMFNRTRCLSCGACQDHTLPDACPSGALETCGKCMETGEILELALREK
                     LFFDRSGGGITCTGGEPLMQPEFLVSLLAACKENNIHTAVDTCLYAPEKVVQQVIPYT
                     DLFLADLKVMNPALHKQYTGADNALIHSNLRTVAHSRVPFALRIPLIPGVNDTKTELE
                     AMAAFALELQRAGNLTTIHLLPYHDYGKEKRSRILRGRAPFQGNFSSPPEKQVRAILN
                     DFHRQGLPAFRGG"
     gene            complement(7831..10266)
                     /locus_tag="PLM68_00865"
     CDS             complement(7831..10266)
                     /locus_tag="PLM68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOT53956.1"
                     /translation="MKSSIPGDSISQRIARLRQASYQTPPSLSVERAQIVTRFYRENQ
                     DRYPTPVLRALCFRELCLKQSIYIGDEELIVGERGPRPRAVSTYPELTCHTIEDLKTL
                     NARELQHYTVTGEDIRIYKEEIIPFWEGRTQRDKLFSILPRDWKLLYESGLFTEFAEQ
                     RAYGHTALDGKIYHTGMLAFRERIAEALAAAGPEADTPAGEAGADAAAGTPAHSRKDM
                     LQAMDIACQAIVVFAERHAALAEEMAVACRSEGNNGRAAELEEIARICRRVPALAPQT
                     YHEAIQAYWFVHLGTIMELNGWDAMNPGHLDQHLEPFYLKETQAGTLSDTFARELMAC
                     LFIKVNNHPAPPKVGVTAKESGTYNDFTNINIGGLRPDGTDAVSPVSYIALDTLEELH
                     LLQPGLSVHIASCTPERFVAASTRVIGKGYGYPSVFNPDVYIRELLRQGKTLPDACEG
                     GCSGCVEVGAFGKEAYLLTGYLNVAKVLEITLYNGVDPVLGRQVSLQTGPATGFRDFE
                     ELYQAFLEQLRYIIDTKIRVNLLIEKLTATGTPAPFLSVLIDDCIQKGRDYYDGGPRY
                     NTSYIQCTGLGSIADSLASLKKHVFEDKKWTMEQMLRAMSCNFEGEEPMRQFILNKTP
                     FFGNDLDYADDLAVRVYDDLFRLIEGKPNARGGVHHMDMLSTTCHVYFGTITGALPNG
                     RKAGKPLSDGTSPSQGADTKGPTAVAHSLGKLDQSLSGGTLLNQRFAPSMLKTAEERK
                     KLGDLIRCYFELGGHHIQFNVVDTKTLLAAQKEPERYRDLMVRVAGYSDYFNHLAPSL
                     QEDIITRTLNEGF"
     gene            10373..10753
                     /gene="rpsL"
                     /locus_tag="PLM68_00870"
     CDS             10373..10753
                     /gene="rpsL"
                     /locus_tag="PLM68_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT53957.1"
                     /translation="MPTIQQLVRKGRERVIEKSKSRALDACPQKRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHVLRGAL
                     ETSGVEGRTQSRSLYGAKRPKAKK"
     gene            10774..11250
                     /gene="rpsG"
                     /locus_tag="PLM68_00875"
     CDS             10774..11250
                     /gene="rpsG"
                     /locus_tag="PLM68_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT53958.1"
                     /translation="MRKTQPKKRILLPDPKFNDVLVTRFVNNLMLQGKKSIAYGIFYD
                     ALDIVGEKMKDSEKAPLDIWKKALENITPQVEVKSRRVGGANFQVPTEVRPERKISLS
                     MKNMIIYARKRSAHSMAEKLAAEIIAAYNSEGAAFKRKEDMHKMAEANKAFAHFRF"
     gene            11278..13404
                     /gene="fusA"
                     /locus_tag="PLM68_00880"
     CDS             11278..13404
                     /gene="fusA"
                     /locus_tag="PLM68_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT53959.1"
                     /translation="MSRDLQYTRNIGIMAHIDAGKTTTSERILYYTGKTHKMGEVHEG
                     AATMDWMVQEQERGITITSAATTAFWNTCGNKYQINIIDTPGHVDFTVEVERSLRVLD
                     GAVATFCAVGRVQPQTETVWRQADKYRIPRLAYVNKMDRVGADFLAVVDEIHHKLGAN
                     AIPISLPIGTEETYKGIVDLISLKAYIYNSDSKELINYREIEIPSSMREEVEYWRGKL
                     IEAAAEFDDSLMEKFFDNPGSIEKEEVKSALRKATLAMKIVPVTSGSSLKNKGIQFLL
                     DAICQYLPSPMDIDDVTGVNPYTQKTEKRAPSSEEPFCGLVFKIATDPYVGRLAYVRA
                     YSGILESGKTVLNVRTGKRERVTRLYQMHSNKQNPIEVLEAGDIGSAIGFRDLRTGDT
                     ICTDKNPIMLEAITFPDPVIGLAVEPKTQKDLDKLGIALSKLAEEDPTFTVRTDVETG
                     QTIISGMGELHLEIIVDRLKREFGVEINQGAPQVNYRETILGTVSHREVFKKQTGGRG
                     KFADIAFTLEPADPGVTGLQFVDQVKGGNIPREFIPAVEKGFKAAMENGVLAGYPVTA
                     MKVTLTDGSYHTVDSDTLSFEICARQAFRKAAPKAQPALLEPIMSLEVVTPEEYMGDI
                     IGDINKRRGQVNDMDTKGNARIIKAKVPLAEQFGYITTLRTLSSGRATSSMEFFQFEE
                     LPANLAREVMEKTGWAKGFHASSVEF"
     gene            13429..13734
                     /gene="rpsJ"
                     /locus_tag="PLM68_00885"
     CDS             13429..13734
                     /gene="rpsJ"
                     /locus_tag="PLM68_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT53960.1"
                     /translation="MSQKIRIKLKSYDHMLVDKSADKIVKTVKATGAVVSGPIPLPTN
                     KKIFTVNRATFVNKKAREQFELNSFRRLLDIYSSTPKTVDALMKLELPSGVEVEIKV"
     gene            13802..13876
                     /locus_tag="PLM68_00890"
     tRNA            13802..13876
                     /locus_tag="PLM68_00890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13837..13839,aa:Ile,seq:cat)
     gene            complement(14105..14674)
                     /locus_tag="PLM68_00895"
     CDS             complement(14105..14674)
                     /locus_tag="PLM68_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOT53961.1"
                     /translation="MFPAIAQTPWWKAILVAVIGMTLLPVAGGGWFDVRGWGENANTV
                     IGGWNVYQPQQAYIGLTRLLFPFFCGLLLSRFHAHIKVKGGFWWCAVLVAAILAMPYI
                     PGGIKGTPGSLNGWYNALAILVLFPLIVAMGAGSTITEKIGPKVCRFFGDISFPLYIT
                     HYPLMYCQMAWAMNHSLYDRSGVMIGSLG"
     gene            complement(14679..14978)
                     /locus_tag="PLM68_00900"
     CDS             complement(14679..14978)
                     /locus_tag="PLM68_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HOT53962.1"
                     /translation="MDNLIENAIRPLVLGRKKYLFCGNGDAAVRASMVYSLLGSCKAA
                     GVNPEQWLEDVLSKIYLYSTGKGNMEDLLPANWAKSNSVNYLSGAVRVAAVLFAG"
     gene            complement(15227..16522)
                     /gene="creD"
                     /locus_tag="PLM68_00905"
     CDS             complement(15227..16522)
                     /gene="creD"
                     /locus_tag="PLM68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HOT53963.1"
                     /translation="MKIEITNQGKWVNSLTLKIALLAVLSLFLLIPLQMIKDIIEERK
                     ANAEKVQEEISNEWANSQCFTGPVLNIPVRSVSPEDQAKTITQIWHILPAEMIIEGEI
                     LPGIRYRSIYQSVVYDANLKIKGAFTVPAESASPGAEVLWNQAYFTVGISDNRGLKGE
                     IAMQAGTRTLSAEPGVKDQDVFTSGISFAGLELKPGSTVAFDLNIDLAGSNTLKLTPL
                     GKNTNVSLRSVWNSPGFCGSFLPVTRVVDRSGFTADWEVTHLNRNFPQDWIGNAFQPE
                     ESAFGVNLVQPVDHYQKSWRSARYGILFIVLTFLVFLFIEIRGKKTIPVIHYVLVSLA
                     LVLFFSLLTALSEQLGFNAAYLIASGATIVLISVFTALVIKDTKTPWLVFSLLLLLYA
                     FIYVLLTLNDYAFLAGNIGLFLILAVVMGVAGKWSLGHN"
     gene            complement(16575..16871)
                     /locus_tag="PLM68_00910"
     CDS             complement(16575..16871)
                     /locus_tag="PLM68_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOT53964.1"
                     /translation="MKDLIANLNKIFESRVRLGIMSVLTVNETYDFNSLKETLDLTDG
                     NLASHLKALEKEGMIVVEKQFIDRKPNTSYSVTEKGRTAFSEHLSALENLIREP"
     gene            complement(16868..17440)
                     /locus_tag="PLM68_00915"
     CDS             complement(16868..17440)
                     /locus_tag="PLM68_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53965.1"
                     /translation="MKNEPNTHEDIKAIRKLMEESTRFLSLSGLSGVIAGVTALAGAL
                     LAWLFIDQTWSLAGVALIVLVISLSSAYFLSLKKARRDKKHFWTPVSRKMLLNLFIPL
                     VTGGVLALIFLLQGNLQMVMPVFLIFYGLALVNASKFTLGEIFFLGLFEIVTGLIVTI
                     FPNLWLIGWSVGFGLLHIVYGLVMYGKYGK"
     gene            18916..20088
                     /locus_tag="PLM68_00920"
     CDS             18916..20088
                     /locus_tag="PLM68_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT53966.1"
                     /translation="MISLDIQREDQQDQGVLLHCSGRLDATSTDHLTDYVERLVREGK
                     YQVALDLSRIEYMSSAGIRALVVQHKNLQSLNGHFYIIRSSDNVAQVLAMVGMSDMFS
                     PPDQKAAPAKEEIQDYITEEDGYVFRMQELDKQASTKVTCSGEPQRIADGDYRQEDTR
                     IIASARGKFALGLGAIGESFPECSGRFGEYLLLNGMAAYLPADGSKKPDYMISHGKLV
                     ARLTEVYGLHFSGPFSYRVVFEPGQEARGLELSPLARLISRLTGYHAWAFVMIAESAG
                     LIGTSLNLSPVGGRSLFSYPGVKDAVRFTTEPAHLRMQTVSAGIVSGAAEGDPGSAGA
                     PGAEGVPGLPVFTGLPVFPGLKELPDLKMLPDLKMLPDLPVFPVIPERRAGIFYGP"
     gene            20085..20321
                     /locus_tag="PLM68_00925"
     CDS             20085..20321
                     /locus_tag="PLM68_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53967.1"
                     /translation="MKDNTLRAHIHTAVFSYIPLKKNDPSPDDTISHLLEHARLTDIL
                     HLLCDDRPLSGLGESAFLQGVCWVVPVEKFVNDQ"
     gene            20328..21266
                     /locus_tag="PLM68_00930"
     CDS             20328..21266
                     /locus_tag="PLM68_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT53968.1"
                     /translation="MSILITSMIFGLILSFLALGVFISFRVFDFPDITVEGSFTTGAA
                     LAALLIVSGCDPMLSCLVSFAAGMVSGALTGLIHTRFRINPLLSGILVMTALYSVNLH
                     VMGRSNLPMPGAVTLFSGLEKISQQLFGQGSKVLLLGWSVDIKDVVILVTLLVAVSLT
                     GFVLRWFLRTNLGTAMRATGDNDQMIRALGVNTKWMIVFGIALANGFVALSGALMAQF
                     QGFADVQMGIGMMVWGLASVIIGEVVVGRTSVGYVIFGAVLGSVLFRLLVAIALRLGM
                     NPNDLKIITAAFVFIALILPGLMKRRKSLWGRKKYA"
     gene            21259..>22050
                     /locus_tag="PLM68_00935"
     CDS             21259..>22050
                     /locus_tag="PLM68_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT53969.1"
                     /translation="MLELYNIKKTFHSGTVHEVRALNGVSLTLEKGSFVCVLGSNGSG
                     KSTLLNAIAGTFPVDEGTIRLDGTDITAWPEHKRARHMGRVFQNPLSGTAPNMTISEN
                     ISLALRRGKPRGLGPGISHGKKEEYRQMVRPLNMDLEERLDNPIGKLSGGQRQALTLL
                     IASALQPKLLLLDEHTAALDPKTAARVTEITQMIITRDSLTTLMVTHSMHQAVHMGDR
                     LIMMHQGRVAYDFKGEDKKRLKVNDLLALFDDLRRKDAIDPGVAAL"
     assembly_gap    22051..22150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22226..23209
                     /locus_tag="PLM68_00940"
     CDS             22226..23209
                     /locus_tag="PLM68_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT53970.1"
                     /translation="MKSISKSLFILFLVAGVLLLLDLGNREKGKKDALKIAVFKMTTR
                     PSLDDTEDGVFEALSQRGYIDGQGCTIQRFSADGDMVTANAIAQNIVSSRFDMVVTIS
                     TPALQVMANANKKGDVLHVFCTVTDPYVSGVGITGTQPDQHPPHLVGIGTFQPVEETF
                     TLMKQIKPDLKKVGTVWCTSETCSEACVRLARKKCAELGIELLEMSVENTTQILEAAM
                     SLTSRGVEALWVGGDNVVEAAIDQMINAAAKARIPLIINNTNSVYGNTLFGLGAKYTE
                     VGYLAGQMAADVLEGKPTSEIGIRNMVPLHLLVNPDALNNIHDKWKLPEEL"
     gene            23206..23865
                     /locus_tag="PLM68_00945"
     CDS             23206..23865
                     /locus_tag="PLM68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT53971.1"
                     /translation="MKVILHNSMSLDGSFIGIAANMELHYQAVNSFGAQVYMVGSNTT
                     SRESIEQYGETVPPETASDFQKPVRDPALSYWVIPDTKGTLQGILHYHRRFEYCRDVI
                     LLISDATPAGYIEYLEQRNYGYIKTGSVHVNYREAFKQLAQRYGVDTILVDTGSRLGN
                     VLLNEGLVDEISLVVTPEILAKNNRHLFEGVEHHIRLRSKKCETMPDGYIWIVFEIIG
                     H"
     gene            complement(24038..24529)
                     /locus_tag="PLM68_00950"
     CDS             complement(24038..24529)
                     /locus_tag="PLM68_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOT53972.1"
                     /translation="MKINFEIMSFEHRDAVMDIFNYYVSNSLATYPDDVLPYEYYEKF
                     LDMTKCYPAYVIKENSKIVGFCMLRAYNPFPTFKECAEVSYFIDHHHTGKGIGSMALK
                     RLEEDALKVGIKTILANISSINTRSIDFHKKNGFVECGRFQKIITKKGKPLDIVWMQK
                     TLE"
     gene            complement(24541..24951)
                     /locus_tag="PLM68_00955"
     CDS             complement(24541..24951)
                     /locus_tag="PLM68_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOT53973.1"
                     /translation="MECFFENLKTCDFSNGGFLPFLPFIIMCMVFGMYIQALQVVAIW
                     HVFTKAGKPGWACLIPIYDYLVALKIIGKPWWWVILLFIPFVNLIFLIWGYNLLSKSF
                     GKGVDFTIGLLLLPFIFFPILGFGKTEYNGPAGK"
BASE COUNT         6246 a         6088 c         6516 g         6074 t
ORIGIN      
        1 cgggcaatcc ttgacggcca gactgcactg tacaggttgc tggtggaacg gtaccagaac
       61 ccggtcttca gggtgattta caaaattgtc aataatcacg aagaggccgg ggaactgaca
      121 caggatgttt ttgtgaaagc ttatgaatcg ctgccaaagt atgatccccg gtataagttc
      181 ttcagctgga tctacaggat ggcaatcaac agggcactac tgtgcctgag aaccagaaaa
      241 ccctgtgtgg aagctgacaa gatccctgcg catgagagat tggcacaaaa agaagagccg
      301 tttgacctgg ttaccaaaag taccctgata gacaaggcgg taaacgaact tgacaaaaaa
      361 tacaagacag tgatcatgct gaagtatttt gccggcctat cctattccga aatttccgaa
      421 accctggata taccggaaaa aacaataaaa tcacggttgt ttgacgcgcg gatgttgtta
      481 aaggacaagc ttgaaaaaaa actgaaatat gttgactgaa agggacatat tactcataca
      541 gaataaagtg gacgggatgc tctcccaccg cgaagaagat cagtttgcgg ccttgcttga
      601 agcatcaccc caggctgtgg acctttacag gaaactgctg gccgtgcatg aagaaatgta
      661 cagcagtgct gcccgtatcc ctgagataga tataacaaaa caggttatgc gccgtataga
      721 aggcaagaca tttctccaca agctcttcac cggcagcggc agggtatatg cctttgctgc
      781 cgccctggtg ctgttttttg tactgggtat gcttgcggcc acgtatcttg ttccttcact
      841 caggaacctt tcacaggagg agcttgcggg aacgatgatg cgtaaaaaaa cttccctgtg
      901 gtctttccat gatacaggga ttgacattga agtacagcaa cagcaggagg gtgatctggt
      961 aatttacagg gcgtctgttt ttaccgcaga cagtcttaat gtggagtttt cctcacccgg
     1021 ggagccggtg aacgtcaatt tgctgaaaat aatagatttt gacggactgg ctcaaaaaat
     1081 ggaaaatacg gaccataaaa tatcttatat ttgccaaggt aatacggtct ttacactgac
     1141 cgaccctccc gatcgttctt cccttacgtt ttctttggaa gggaaacaag ccttccacat
     1201 gaaatttcca ccaacttttt gacgaccatt gattatttat tatagaagac tacaaacaca
     1261 cacgacaatg aaaacaaaaa acaaatcctc aatcgtattg gtcctggccg ttcttgtgtc
     1321 tcttgctgct ggttttttag tgggaatcct ggtggatatt ccaaaaaccg ataacacaca
     1381 acttgccggc accataggca gggtgcagaa ttacaagaac gtgaaggtta cggaggaaga
     1441 cattgaactc aagaatgacc tggtttcgga cacgctcata ctcaatgcca tttccaccta
     1501 tttcaattac cattatgttg ccgccgtgtc ccagggagaa aagatccggt atgcactgga
     1561 tgcccttgat aatcaagacg cctataggga atatgccggc ctgctgctgc aggaagtgtc
     1621 aggttatggg acattcctgg aaaatgcccg gcttgatctg ctccaggcca tagccgtggt
     1681 cacgaatcct gccgaggtac acccggtaat gatgcgcaat acgatagtgc aggcaaacaa
     1741 tgtgatctcc cagatcagtt accggaaaca atcggtactg aacctgatag acaacctggg
     1801 cagttacctg gaaaagcagg acaaggaggc cgatgccaca ctggcatctg tgcacgcggt
     1861 actgagcctg gaacaggtca ccaacgccat ggcgctgaat gacaagatgg tgttgaaata
     1921 tttcgagaag aaaaaaatat tcacagaaca gatccaggga tcttttggct cggaactgca
     1981 gggtctcatt gtagaagatg cccagaggct gggaattgat cttaacaata ttgaccccat
     2041 gggaataatt atccttaacg cagaaagcat tggcagccat tttgccttga acaatattga
     2101 ggttggttca atgatcaaca acatgaacga gctgggggtt tttggatctg aggcagttag
     2161 aagctccaac gacttgggga gcgtagcgtt catgaacctg gaaaatcttt cgcttcaaat
     2221 attcgggagc tcggaaaacc tggaggctgt tcttaatgcc cccgacctgg ggatgtttaa
     2281 caatatgcag gaactcggat tcctgaatgt aaaccagttg aatgttgtaa tgagggtgag
     2341 tgaattggga ggggttattg gaatcgggcc aataggatat taatgaaaat aaattgaaaa
     2401 agttcaaagc catcatatta ttaatggttg taattatccc gggcccgccc gggattttgc
     2461 gtgcacagct tattaacagc gttaaaaaag acaacctgca cctggtctat ttcggtaaca
     2521 ggtattccta tatagtgccg catgtaagac agacttacct caatgccatg gattttcaca
     2581 agcgcttctg gtcttaccgg gacacgacaa cgtatgtttt ccttacggac tttgaagata
     2641 caggacatgg aggagccatt gccatgcctt ataaccaggt catcctgggg attgagccct
     2701 atagtctggc attcagtatc atcccttcca acgaacggtt ccaatggctg ttcaaccatg
     2761 agctgacaca tattgtactc tctgaaaaac ccaatacaag ggacgctaaa tttcgcaaat
     2821 tcctccaggg aaaggtgata agggccgatg agtaccccat atcggccctg tggagttatt
     2881 ttaccacccc ccgctggtat gccccacgct ggtaccacga ggggatagcc tgcttcatgg
     2941 aaacctggat gtcaggggga ttcgggaggg ccatgggttt ttatgacgag atgtacttcc
     3001 gcagcatcat tgaaggcaag gaggacctct attccctggt agggctggat acggaaggaa
     3061 ccacagagga tttccaggtg ggagccaatt cctatctgta cggcacccgt tttgtgacct
     3121 acctggccta ccaatacggg atagacaaac tgaaggactt ttatatccgt tccgacagtt
     3181 cccgggcatt ctatgcaggg cagttccgga aaatttacgg acgcccggtc agggaggtct
     3241 ggcaggagtg gatggaatgg gagcaggatt atcagcagaa aaccattgcc cgtatcgggg
     3301 aatttccact gacaaccctg cgctctctta ccgaagaacc tctgggcagt gtttccaggc
     3361 cggaattcaa cccggagacc gggaaaatct atgccgccat caactatccc ggcattattt
     3421 ctcaaattgc cgagatagac aggaccaccg gcagtatccg gaaggtggcc accctggatt
     3481 ctcccaaact gtattatacc acacacctgg cttacaatgc ggaggacgag aagatctata
     3541 cttccgaaca caacagcaat taccggaatt tggtggaaat cgacgtgaag acgggaagca
     3601 aaaaggtgct gaacaaactc acccgggccg gtgacctggt cttcaacccg gcagacaagt
     3661 ccttgtgggg gatccagaac gacaacggat actccatact ggtgaagatg ccaccgccat
     3721 acgacaaggc actgcctgtc tttactgttc cttttggtcg gatcctactg gatccggata
     3781 tttccaattc gggaaaatta ttatgcgcta ccctgtcggg cataagaggg gaacagtccc
     3841 tggtactgtt tgatctggaa gcgctggatg ccggcaagag cagtatggaa accataatgg
     3901 aactggaaga caacacactt acgcagttca ggttttctgc cgatgaccgc tttctgatag
     3961 gaacgtctta ctatacgggt gtttccaacg tatggaggat aggacttgaa gacaggaaca
     4021 tggaattgct atccaattcg gctaccgggc tttttgcccc aatccaggtg acggaagatt
     4081 cactgctggt gcttgaatac gaaagggacg ggatgcgtcc cgggatcatg cccgtcacgg
     4141 ttctggaaga cgccaatgcc atcagcttcc tgggcaacat ggtccaccag gcccaccccg
     4201 aggtggaaga gtggtccctg ccccccgcct cggccctgga aggggtcaac gattccatta
     4261 tcgagacacc ctacaggccc cttaaagaaa tgaagatcgc aggggcctac cccgacattg
     4321 ccggtttcaa gaataccgta gccctggggt accggctcaa ctggcgggac ccggtgggga
     4381 tcagcaatat caacctgttc ctggcggtat cgccatggag ttcctatgga atagaacaaa
     4441 agttccatgc catgctggaa tggaaattct ggagctggcg cttgaaagcc agttataacc
     4501 caacccactt ctacgatatt ttcggaccca caaaaagaag cagggccggt tattccattg
     4561 gcctgggata ccaacggact ttcatcctga aagatcccct gaagtatcat gtagatgctt
     4621 ctgttttcac gtatggcatg ctggaagttc ttccggttta ccaggagatt gcatctccca
     4681 taaggaacat gcaggcagcc tctgtcacct ttggtgtatc caagctgcgc aagaccctgg
     4741 gaggtgtgga cgacgagatg ggtttcacct gggatataaa cgccaccacc tacctggcac
     4801 aaaagagatt ctacccctcc tttgtttcgg atcaaagcgt gggcttcctg atacctttca
     4861 tccggaacac cagtttctgg atacgcaaca gcgtgggcat ctcgctgggt gatcgtgaat
     4921 ccagcttatc ctatttctat tttggaggat tcgggaacaa ttatgtggac tggcagccgt
     4981 ccgagcaata ccgcaataca ttgtcttttc ccggggcccg tataaaccag ttgccggcct
     5041 atacgtatgt caggacaatg ggggaactta acctcatgcc catcaggcta cgcaatgtag
     5101 gcaccacgtg gctgtatccc acctacatca aaccttccct atacggggct cacctgatga
     5161 ccggatttga ccgcccccag cagacggctc atttgttcaa ttgcggcgga caaatagata
     5221 tccagctggt cttgttttct tatctcaaga caacctggtc tttcggttac gctaaaatgt
     5281 tccgggcagg tgaaaaatca accaatcaat tcatgttgtc actaaaactg cttggaaatt
     5341 agttgcccat gaagaataag ttcccggctc cgctggtcat tgtctttgtg cttattgtgg
     5401 ttgcagccat gctaacctgg tttttgcccg gtggacagta tgttgaaggg acatttcaac
     5461 cggcagactc ccgtccccag acgtggcagg tattcacggc tttttacaac gggtttgtca
     5521 gacaggctgg gatcattgta ttcattttat gcgtgggggc tgccttctgg gtaatcaatg
     5581 caacccgggc cattgacagc ggcatccggt cttttctgga cagcgcggcc cggctggaac
     5641 gtttcccgct gttgcgccgc attggggtga ataacctggt gatcgcctct gtgatggtcc
     5701 ttttcagtct ttttggcgca gtcttcggga tgagtgagga aacaatagcc tttacagccc
     5761 tggtggttcc actggcctta tccatgggtt atgattccat aaccggggta tgcatggttt
     5821 acgttgcggc ccatgtgggt tttgccggag ccttcatgaa tcctttcacc gtgggcattg
     5881 cccaggatct gtcggacctg cccatatttt cgggcatggg ataccgcctg gtttgctggg
     5941 ccctcctgac agcgggaacc attgtctttg tgctgcggta tgcccataaa gtgaaacggg
     6001 atccaaaagc ctcgcccata tgggaagagg acgcttcccg caaagttgcc catgaggccg
     6061 gggtggacct gttagcagaa taccctgctg aaggggcaaa aaccacatgg acagcctgga
     6121 tggctttctt cctggtgctg gccgccctga ccgccttttc cgtttcctct ccttccacca
     6181 tgatagcctt gggagcctgg aagctgaaaa tccccgggct tcttcccgcc cttacatttc
     6241 tgtacgctgc aggagggttg aaaatcctta cgggaaagga caaacaggag aatcctgccc
     6301 gccttgtccg tcattttgtg ctattcgctg ttcttttcct gctggttggc gtcttgggct
     6361 acaactggta tataaacgag attgccggtc ttttccttgc cctggggata ctgagcggaa
     6421 tggcagccgg ctacagggca aacaccattg cgcaacagtt gacagaaggc gccagggaca
     6481 tcctgggagc agcgctgatc gtgggattgg ctgccgggat tgttgagatc ctggaacagg
     6541 gaaggatcat ggacaggata ctttttgcca tggccggagg cctggaatct gtcggacgga
     6601 cgggttcgct gcaggtcatg tacttgatcc agacggccat caacatgctc attccctcgg
     6661 cctcggccaa agcggccatg actatgccca tcatggcgcc tttcagtgac ctggtggggg
     6721 tctcgcgaca ggccacggtg ctggctttcc agttcgggga tggatttacc aatatgatca
     6781 cgcccatatc cggggtgctg atggctgtac tgggggttgc gcgcattccc tatacccggt
     6841 gggccaggtg ggtctggcgc tttatcctgg gactgatagt gaccggagcg ttgctgctgc
     6901 ttcccacact tttcatctgg aagtaacgtt gctcccggaa ggacggctgt cctgccttcg
     6961 tcgacgggat tggtttaacc gcccctgaat gcagggagtc cttgtctgtg gaagtcgttg
     7021 agtatggccc ggacttgttt ttccggcggg gaggagaagt tcccctggaa aggagccctg
     7081 ccccgcagga tccgggacct tttttctttt ccgtagtcgt ggtaggggag caagtggatg
     7141 gtggtcagat tgcccgcgcg ttgcagctcc agggcgaagg ctgccatggc ctccagttcg
     7201 gtttttgtgt cgtttacccc ggggatgagg gggatgcgca gggcaaaggg taccctggaa
     7261 tgggcgacgg tacgcaggtt gctgtggata agggcgttat cggccccggt gtattgttta
     7321 tgcagggcag gattcatcac cttgaggtcc gcaaggaaca ggtctgtgta tgggatcact
     7381 tgttggacaa ctttttccgg ggcgtagagg caagtatcca cggccgtgtg gatgttgttt
     7441 tccttacagg cggcaagcag gctcaccaga aattccggct gcatgagggg ttcaccccct
     7501 gtgcaggtaa tcccgccccc ggagcggtca aagaataatt tttcccgcag ggcaagttcc
     7561 agtatttcgc ctgtttccat gcacttacca cacgtttcca gggccccgga cgggcaggca
     7621 tcgggcaggg tgtgatcctg gcaggcccca catgacaggc aacgggtacg attgaacatg
     7681 accacaggct ccgggtcttg tccctcggga ttatggcacc actggcagtg taacgggcag
     7741 cccttgaaga acacattcag gcgtatgccc ggcccgtcat gtatggcgta gcgtctgata
     7801 tcaaaaatgc aacctttgga tgtcatggat tcagaaaccc tcattcaggg tacgggtgat
     7861 gatgtcttcc tgcagggaag gagccaggtg gttgaagtag tcactgtatc cggcaaccct
     7921 taccatcagg tcccggtagc gttccggttc tttttgcgca gccagcaggg ttttggtgtc
     7981 caccacgttg aactggatgt ggtggcctcc cagttcgaag tagcaacgga tcaggtcacc
     8041 cagttttttc ctttcttcag ctgttttgag catagacggg gcaaaacgct ggttcagcag
     8101 ggtgccgccg gaaagcgact ggtccagttt tcccagggag tgcgcaacag ccgtgggacc
     8161 tttggtatct gccccctggg acggggaggt gccgtccgag agcggtttcc ctgccttgcg
     8221 gccgttgggc aacgccccgg ttatggtccc gaagtacaca tggcaggtgg tggagagcat
     8281 gtccatgtga tggactcctc cccgggcatt gggctttcct tcaatcagcc ggaacaggtc
     8341 atcgtaaaca cgcaccgcca ggtcgtctgc atagtccagg tcgttgccga agaacggggt
     8401 tttattcaga atgaactggc gcattggttc ctccccttca aaattgcagg acatggcccg
     8461 gagcatctgt tccatggtcc atttcttatc ttcgaagaca tgcttcttca ggctggccag
     8521 gctgtctgca atgctgccca ggcccgtgca ctggatgtag gaggtgttgt aacgcgggcc
     8581 cccgtcgtaa taatcccttc ctttttgaat gcagtcgtct atgagaacgg agaggaacgg
     8641 ggccggtgtc ccagttgccg tcaatttttc gatgagcagg ttcacccgta tttttgtatc
     8701 tatgatgtac cggagttgtt ccaggaaagc ctggtataat tcttcaaaat ccctgaaacc
     8761 ggtggccggg cctgtctgca gtgaaacctg ccggcccagg accgggtcca cgccgttata
     8821 cagcgtgatt tccagcacct tggctacatt caggtacccg gtaagcaaat aggcctcttt
     8881 accgaaagca cccacttcca cgcaaccgct gcagcctcct tcacaggcat ccggcaaggt
     8941 ttttccctgg cgcagaagct cccgtatata cacatctgga ttgaacacgg aagggtaacc
     9001 gtatcctttg cctatgaccc gggtgcttgc cgcgacaaag cgttccggtg tgcaggaggc
     9061 aatatggaca gagaggcccg gttggagcag gtgaagctct tccagcgtgt ccagggcaat
     9121 gtaggaaaca gggcttacgg catctgtccc gtcgggtcgc agaccgccga tattgatatt
     9181 ggtaaagtcg ttgtaggtcc cgctttcctt tgcggtaacg cccaccttgg gaggggcggg
     9241 gtggttgttc acctttatga aaaggcaggc catcagttcc cgggcaaagg tgtcggataa
     9301 cgtccctgcc tgtgtttcct tcagatagaa aggttccagg tgctggtcca ggtgtccggg
     9361 attcatggca tcccagccgt tcagttccat gatcgtgccc aggtgtacga accagtaggc
     9421 ctgtatggct tcatggtagg tttgtggggc aagggcgggc acgcgccggc atatgcgtgc
     9481 gatttcttcc agttcagctg ccctgccgtt gttaccttcc gagcggcagg ctacagccat
     9541 ttcctctgcc agtgctgcgt ggcgctctgc gaaaacaaca atggcctgac aggcaatatc
     9601 catggcctgg agcatgtctt tccggctgtg tgccggggta cctgctgccg catctgcacc
     9661 tgcttcccct gccggggtgt ccgcttccgg tccggccgca gccagcgcct cggcaatccg
     9721 ctccctgaaa gcgagcatcc cggtatggta aatctttcca tccagggcgg tatggccgta
     9781 agcccgctgc tcggcaaatt ccgtaaaaag tccgctttca tacaacagct tccaatccct
     9841 gggaaggatg gaaaagagtt tgtccctttg tgtcctgcct tcccagaaag gaatgatctc
     9901 ttccttgtag atcctgatgt cttccccggt cacggtatag tgctgcagct ccctggcatt
     9961 caaggtcttc aggtcctcta tagtgtgaca cgtaagttcg ggataggttg agacagccct
    10021 gggccgcggg ccgcgttcac ccactatcag ttcttcatct ccaatgtata tggattgttt
    10081 aaggcataat tccctgaaac ataaagcccg tagaacaggt gtcgggtatc ggtcctggtt
    10141 ttcacggtaa aaacgggtta cgatctgtgc cctttcaaca cttagggaag gaggtgtctg
    10201 gtaagatgcc tggcggagcc tggctatgcg ctgactgatg gaatctcccg gaatgcttga
    10261 tttcatgagt gtaaaagtag aagatatttt tttaattcag aaaaataatc ctatctttgc
    10321 aacccgtttt acgtacaaga aaaatacata aagcaaagat taacaacaca ttatgccaac
    10381 aattcaacaa ttagtccgta aaggaaggga acgtgtgata gaaaaaagca aatcgcgcgc
    10441 tttggacgcc tgtcctcaga aacgcggcgt ttgtgtacgt gtctatacca ccacccctaa
    10501 aaaaccgaac tctgccatgc gtaaggttgc cagggtacgg ctgaccaatc agaaagaggt
    10561 caatgcctat atccccggag aaggacacaa ccttcaggag cactccattg tactggtgcg
    10621 cggaggacgt gtaaaggacc tgccaggtgt tcgctatcat gtgctcagag gcgctttgga
    10681 aacctctggt gtggaaggca gaacacaatc ccgttcgctg tatggagcaa aacgccccaa
    10741 agcgaaaaaa taaccattaa ttgattaaca ccaatgagaa aaacacagcc taaaaagagg
    10801 attctacttc ctgatcccaa gttcaacgac gtgctggtta cacgttttgt gaacaatttg
    10861 atgcttcagg ggaagaagag tattgcctac ggtatttttt acgatgcatt ggatatcgtg
    10921 ggtgagaaaa tgaaagattc tgagaaggct cctttagaca tttggaaaaa agcgttggaa
    10981 aacatcaccc cgcaagtgga agtaaaaagc cgccgcgtgg gaggagccaa cttccaggtg
    11041 cctacagaag tgcgtcctga gcggaagatt tcattaagca tgaaaaacat gatcatttac
    11101 gcccgcaaac gttcagcgca ttccatggcc gaaaaactgg ccgccgaaat catagctgcc
    11161 tacaacagcg aaggtgccgc gttcaagcgt aaagaagaca tgcacaagat ggcagaagcc
    11221 aacaaggctt ttgctcattt ccgcttctag acagggagtg tcttttagca cctgaaaatg
    11281 tcaagagatt tacaatatac aaggaatatt ggcatcatgg cccacataga tgccggtaag
    11341 accaccacat cggaacgtat tctctattat acgggtaaga cccataaaat gggggaagtt
    11401 catgaggggg ctgccactat ggactggatg gttcaggagc aggaacgggg catcaccatc
    11461 acctcggctg ctaccacagc gttttggaac acctgtggga acaaatacca gatcaacatc
    11521 atagacaccc ccggccatgt ggattttacc gttgaagtgg aacgttccct gcgtgtgctt
    11581 gacggcgccg tggcaacgtt ctgtgcagtg ggacgtgtgc agccccagac agagacagtc
    11641 tggcggcagg cagacaaata ccggatcccc cgcctggctt atgtcaacaa aatggaccgg
    11701 gtgggagccg atttccttgc tgttgtggac gagatacatc acaaactggg tgcgaacgcc
    11761 attcctataa gccttcccat tgggacagaa gaaacataca agggcattgt tgacctgatc
    11821 tccctcaaag cttatattta caatagcgat agcaaagagc tgatcaatta cagggaaatc
    11881 gaaattcctt catccatgag ggaggaggtt gagtactgga gggggaaact catagaagcc
    11941 gctgcggaat ttgatgattc cctgatggaa aaattctttg acaatcccgg atccatagaa
    12001 aaggaagaag tcaaaagcgc cctgcgcaag gctaccctgg ccatgaaaat tgttcctgtc
    12061 accagtggtt cttcactcaa gaacaaaggg atacaattcc tgctggatgc catctgtcaa
    12121 taccttccct ctcccatgga tatagacgat gttacgggcg tcaatcccta cacccaaaag
    12181 accgagaaaa gagcgccttc ttccgaagag cctttctgcg ggctggtatt caagattgca
    12241 accgatccct atgtaggcag gctggcttat gtaagagcct attccggaat actggaatcc
    12301 gggaagacag tgcttaacgt tcgtaccggg aaacgtgagc gcgtaacccg tctgtaccag
    12361 atgcactcca acaaacagaa ccccatagag gtccttgagg caggggatat tggcagcgct
    12421 atcggattca gggatttacg caccggagac accatttgta ccgacaagaa tcccattatg
    12481 ctggaagcca ttacctttcc ggatccagtc attggactgg ctgtggaacc aaaaacgcag
    12541 aaagacctgg ataaactggg catagccctt tccaaacttg ccgaggagga tcccactttt
    12601 accgtccgta ccgatgtcga aaccggccag accatcatca gcgggatggg cgaactgcac
    12661 ctggagatca ttgttgaccg gctcaaacgg gaattcggcg tggagatcaa ccagggtgcc
    12721 ccacaggtca actaccggga aaccatcctg ggaaccgtct cgcaccggga agttttcaaa
    12781 aaacaaaccg gcggacgcgg aaaatttgcc gatatcgcct ttaccctgga accggccgat
    12841 cccggtgtta caggactgca attcgttgat caggtcaagg gcgggaacat acccagggaa
    12901 ttcattcctg ccgtggagaa aggattcaaa gctgccatgg aaaacggcgt gctggccgga
    12961 tatccggtca cggcaatgaa agtgacccta acagacggtt cttaccacac tgtggactca
    13021 gataccctgt ccttcgagat ctgtgcccgc caggccttcc gcaaagcggc ccccaaggca
    13081 caacctgccc tgcttgaacc catcatgtca ctggaagtcg ttacacccga ggaatacatg
    13141 ggagacatca taggcgacat aaacaaacgc cgcggtcagg tcaacgatat ggacaccaaa
    13201 ggaaacgccc ggatcatcaa ggcaaaggtt cctttggcag aacaattcgg ttatatcact
    13261 accttgcgga cgctttcctc gggccgtgcc accagctcca tggaattttt ccagttcgag
    13321 gaattgcctg caaacctggc cagggaggtc atggaaaaga ccggatgggc aaaaggattt
    13381 catgcatcta gtgttgaatt ttaatatact tggttaataa ttattacaat gagccaaaag
    13441 atcagaatca aattaaaatc ttacgatcac atgctggtag acaagtctgc cgataagatc
    13501 gtaaaaacag tcaaagcaac cggcgctgta gtgagcggtc ccattcccct gcccaccaac
    13561 aagaagatct tcacggtcaa ccgcgccact tttgtgaaca aaaaagcccg ggagcaattt
    13621 gaattgaact ctttccgccg tctgctggat atttacagct ccacacccaa gacggtagac
    13681 gccctcatga aactggaact gcccagtggc gttgaggttg aaatcaaggt ctgacgcgtt
    13741 acgttctttg atcgggattt tacattaaca tacctttcgt gtatatttgt acacgaaaag
    13801 ggggcccata gctcagtcgg ttagtagcac ctgactcata atcagggggt cgctggttca
    13861 agtccagctg ggcccacgga ctgaaaggca cttgcgcatt gcgcaggtgc ctttttttgc
    13921 gcagcgggcg tgcgcagcgg gcgtgcgcag gcaaacccgg tttgccgcgc aagaagattt
    13981 ttgagatttt caaatgtctg atattctgct atttattttt tgcaaatttg gcgttgcgca
    14041 gtaaaccggg tttgcctgaa aagcccactg cgagaattaa gagcccaacg gaaaccatca
    14101 cgtgctagcc cagagagcct atcattacac cgctccggtc atacagggaa tgattcatgg
    14161 cccatgccat ctggcagtac atcaggggat agtgagtgat gtaaagcggg aaagagatgt
    14221 caccaaaaaa cctgcagacc tttgggccga tcttttcggt tattgtgctg ccggccccca
    14281 tggccacgat cagtggaaac agtaccagta tggcaagcgc gttataccat ccgttcaggc
    14341 ttccgggcgt tcccttgatt ccgccgggga tatagggcat ggcgaggatg gccgccacaa
    14401 gaacagcgca ccaccagaag ccacctttta cctttatgtg agcatggaac cgggaaagga
    14461 gaagcccgca gaagaacggg aaaaggagtc tggtcaggcc gatgtatgcc tgctgcggtt
    14521 gataaacgtt ccagcctccg attacggtat tggcgttctc gccccagcca cgcacgtcaa
    14581 accagccgcc gccggcaacg ggtaggagtg tcatgccgat aaccgcaacc aaaatggctt
    14641 tccaccacgg cgtttgggca atagccggaa acatgacgct atcctgcaaa aagaacagca
    14701 gcaacccgaa cagcccctga caagtagttt acagagtttg attttgccca attagctggg
    14761 agcagatctt ccatgttgcc ttttccggta ctgtacaagt agattttgga aagaacatct
    14821 tccagccact gttcggggtt cacaccggct gctttacagg agcccagaag cgagtagacc
    14881 atggaagccc ttacggctgc atcgccgttt ccacagaaca gatatttttt ccttcccaaa
    14941 accaacggac gaatggcgtt ttcaatcaga ttatccaaaa gtttgtataa tctgatggaa
    15001 agcaactaac gacagtgtta ccaactcaag tagttttaca ggccttccag gtgggttccg
    15061 taacagtggg gggcataagt acctataata ccgaagatgg ttattggtgg agttctaccc
    15121 tgtacagttc aaaagcctgc taccggggcc tgttttatac tcacgccaaa gtgcacggaa
    15181 actacggctc aaaagccttt ggtttttacg tacgttgctt taaggattaa ttgtgcccca
    15241 ggctccattt gccggctaca cccataacga cggccagtat caggaacagg cctatgttgc
    15301 cggcaagaaa ggcgtagtca ttcaaggtga gcaatacgta aatgaaagcg tagagcaaca
    15361 aaagcaggct aaaaaccagc cagggtgttt ttgtgtcttt gataaccaat gcggtgaaaa
    15421 cagagatcaa aacaatggtg gctccggagg ctattaagta agcggcgttg aagcccagtt
    15481 gttcacttaa ggcggttaac agggagaaga agaggaccag ggccagtgat accaatacgt
    15541 agtgaatgac agggatggtt tttttgcctc ggatctctat gaaaagaaac accaggaagg
    15601 taaggacaat aaaaagtatc ccgtaccggg cacttctcca ggacttctgg taatgatcca
    15661 ccggttgtac caggttcacc ccaaaggcgc tttcttccgg ttgaaatgcg ttgccgatcc
    15721 aatcctgggg aaagttccgg ttcaggtggg tgacctccca gtctgcggtg aacccgcttc
    15781 tgtccacaac ccgggtaaca gggaggaaac tcccgcaaaa tccaggcgag ttccacacgg
    15841 aacgtaaact gacattggtg tttttcccga gaggtgtcag cttcagggta tttgacccgg
    15901 ccaggtcgat gttcaggtca aaggccaccg tgctgcccgg cttaagctcc aacccggcaa
    15961 aagaaatacc cgaagtaaag acatcctgat cttttactcc cggctctgca ctaagggttc
    16021 gtgtcccggc ttgcatcgcg atttctcctt tcaaccccct gttgtcagaa atgccgactg
    16081 tgaaatatgc ctggttccag agcacctcgg cacccggtga agcggattcg gcgggaaccg
    16141 taaaagctcc cttgattttt agattggcgt cgtatactac agactggtaa atactccggt
    16201 acctgatccc gggaaggatt tccccttcaa taatcatttc tgccggcaga atgtgccaga
    16261 tctgcgtgat ggttttagcc tggtcctcgg gagaaaccga acgaaccgga atattaagca
    16321 caggccccgt aaaacattgg ctgttagccc attcattgga tatttcttcc tgcacttttt
    16381 ctgcattggc tttcctttcc tctatgatat ccttgatcat ttgcaaagga atgagtaaga
    16441 acaatgacaa aacggccagc agggcaattt ttaaagtaag ggaattaacc cattttccct
    16501 ggttggtgat ttcaattttc atgatttatt atatttaaag ttctttgcgt cacaaagtat
    16561 aagggtaaaa atttttaggg ttcccggatt agattttcca aagccgagag gtgctcactg
    16621 aaagcggtgc gtcctttttc cgtcaccgaa tagcttgtat tgggcttacg gtcaatgaat
    16681 tgtttttcca ctacgatcat gccttccttt tccaaagcct tcaggtgact ggccagattt
    16741 ccgtctgtca gatccagcgt ctctttcagg ctgttaaaat catacgtttc attaaccgtc
    16801 aaaaccgaca tgatccccag tctgacccgg ctttcaaaaa ttttattcag gtttgctata
    16861 aggtctttca ttttccgtat ttcccgtaca taaccaatcc atatactatg tgcaacaaac
    16921 caaatcctac ggaccatcca ataagccaca gattcggaaa tattgtaaca ataagccctg
    16981 tcacgatttc aaataagccc aggaaaaaga tttctcccag ggtgaattta gaggcattga
    17041 ccagtgccag gccataaaag atcaggaata caggcatcac catttgcagg tttccctgca
    17101 ggaggaatat tagtgccagc acaccgcctg ttaccagggg aatgaacagg ttcaacaaca
    17161 ttttccggga gacaggcgtc cagaaatgct ttttgtctcg cctggctttt ttaagggata
    17221 aaaaataggc agacgatagt gaaatcacaa gcactatgag ggcaacaccg gccagagacc
    17281 atgtttgatc aatgaacaac caggccagaa gagctccggc aagggcagtg accccggcta
    17341 ttaccccgga caagccgcta agcgatagaa accgtgtaga ttcttccatc agctttctga
    17401 tggcttttat atcctcgtgt gtattgggtt cgtttttcat aataaaaaag tactttgtag
    17461 tgcaaagtta ataaaagttt tcaattatgg tgcaggtgac caaaaaatca ttctcttttt
    17521 ttaagccgag gctgcagccc ttccttgctg gcggtcgctt gactgctaag aacgaagcgc
    17581 ttttttctta agccgaggct taaactgttt gtcacaagca gccgcttcca ccacaagata
    17641 gggaagcctg tttctttttg ccgaggcttc ccaacccttg tgatggcagc gatttttccg
    17701 ttatgcatat tgctgcccca tttgccgtcc agtggtcttt taaggcggat tattcaaggc
    17761 ggtgcgccaa aggcctttgg cgcttataat atgctgctcc gcagcaccaa tcattgttcc
    17821 cggttgcttt gagggaggtg gtaagtaaca attatgttac aaaaacgcat aacagacagt
    17881 atgtgtgcgt gttataagcg tgttcgcgtt ttttcacaac ttactacctc cctggatttc
    17941 agcgctattg ttgggaggtg gtaagtaaca attatgttac aaaaacgcat aacgcgttgt
    18001 ttacgtgtgc attaggtgca tgtctgcgtt tttcatcatt taccacctcc ccctgttttt
    18061 gcggtttttc ggtgtttttg gggagaggtg gtaagtagtt attatatcat aaaaacccgt
    18121 atatcgctat tatgttgtat gttacgagcg tttccgcctt ttcggttact tactaccctc
    18181 cctggatttc atcggttttg cagggaggtg gtaagtaaca actgtgttac aaaagcacat
    18241 aacggcctgt atacgtgtgc attaaaggca aacgtgaatt tctcacaact taccacctcc
    18301 ctgtcaaccc aaactccaaa aaggattaag atctggttgg taaacaaaat ccagggataa
    18361 cctgacttaa gtcaacaatt ttcaggaatg agacattccg tacataaaaa ggggcgtttt
    18421 tgtggatgga attcagcagg ggaccaaagg aggttccacc tcctttgtct ggcgggtgct
    18481 gttgctacga gctaatgtgt gcgaggcact tatctctgtc tctgacacca cctggaacgg
    18541 cgagcctcgg tgtaccccaa aagctcgccg ttccaggtgg tgtcagagac aataggcagc
    18601 ataacagggg caatctcggc actaaaaggg ttcccggtac gttgtgatgg cagcgaccta
    18661 atactggagc aggaggttgt atttgttctg aaaatcctaa gaggttgtct ttcagagtgg
    18721 tgcgctaaaa gctattgacg ccaataatag gctgcgtagc agcactttcc tgatttgctg
    18781 caagtctgcc attgagtagc attattgcac atatgactgt ccggtggtct tcaaggtagt
    18841 gcgacaaacg catttgatgc ataatagtgg aggcaactac tttttattca ccaccattta
    18901 actaaatttg ttacaatgat ttcattggat atacagaggg aagatcagca ggatcaaggg
    18961 gtattgcttc actgttctgg gcggctggat gctaccagta ccgatcatct gacagattac
    19021 gtggagcggt tggtgcggga aggaaaatac caggtagcac tggatctgtc caggattgaa
    19081 tacatgagtt ctgcaggaat ccgggcattg gtcgtccagc ataagaacct gcagtcgctg
    19141 aatggtcatt tctatatcat aagatcttct gacaatgtgg cccaggtgct ggcaatggtg
    19201 ggcatgagcg acatgtttag cccgccggac cagaaggccg ccccggcaaa ggaagaaata
    19261 caggattaca taaccgaaga agacggatac gttttccgga tgcaggagct tgacaaacaa
    19321 gcatccacaa aggtgacctg cagcggggag cctcaaagga tcgcggacgg ggattatagg
    19381 caagaggata caaggatcat tgcctcggcc cggggtaaat ttgcgctggg gctgggagcc
    19441 atcggggaat cgttcccgga gtgcagcggg cggtttggag aatacctgct cctgaatggg
    19501 atggcggctt acctgcctgc ggacgggtcc aaaaagccgg actacatgat aagccatgga
    19561 aagcttgttg cgcggcttac cgaggtgtac ggccttcatt tttcggggcc tttttcatac
    19621 cgggtggtgt ttgagccggg gcaggaagca cgcggcctgg agctttcccc cctggcacgc
    19681 cttatttccc gcctgaccgg ctaccacgcc tgggccttcg tgatgattgc cgagagtgcc
    19741 ggactgatag gcacttcgct caatctttca ccggttggag gaaggtcatt gttttcttat
    19801 cccggggtga aggatgctgt gcgttttacc acggaacctg cccaccttag gatgcagacc
    19861 gtctctgcag ggatcgtatc cggggcggct gaaggagatc ctggatctgc cggtgctccc
    19921 ggggctgaag gagttccggg acttccagtg ttcacgggac ttccagtgtt cccgggcctg
    19981 aaggagctcc cggacctgaa aatgctcccg gacctgaaaa tgctcccgga tctgccggtg
    20041 ttcccggtga ttccggagcg gcgtgcaggg attttctacg gcccatgaag gacaacacgt
    20101 tgcgggcgca catccatacg gcggtattct cgtatattcc cttgaagaaa aatgatcctt
    20161 caccggatga taccataagc catttgctgg agcacgccag gctgacagac atcctgcatt
    20221 tgctctgcga tgaccgtccc ctttcgggtc tgggagaaag tgctttcctg cagggggtgt
    20281 gctgggtcgt tcccgtggaa aagtttgtca atgaccaata aatagatatg tccattctga
    20341 ttacatccat gatctttggc ctgatactgt ctttcctggc attgggggtc tttatcagtt
    20401 tccgtgtgtt cgactttccc gatattacgg ttgaagggtc ttttaccaca ggggcagccc
    20461 tggcggcact gcttattgtg agtggatgcg atccaatgtt gtcctgcctg gtctcttttg
    20521 ctgcgggaat ggtatccggt gcgctcaccg gactcataca cacccgtttc aggatcaatc
    20581 cgctgctttc gggaatattg gttatgacgg cactctactc tgtgaatctt catgttatgg
    20641 gaagaagcaa tttgcccatg cccggggcag tgaccctgtt cagcggattg gaaaagattt
    20701 cgcaacagct cttcgggcag gggagcaagg ttctcctgct gggctggagt gtagacatta
    20761 aagatgtagt catcctggtt accttactag ttgctgtatc gctgaccggt tttgttttac
    20821 gctggttttt gaggaccaac ctggggaccg ctatgcgggc caccggggac aatgatcaga
    20881 tgatcagggc attgggtgta aacaccaaat ggatgatcgt gtttgggatt gcccttgcca
    20941 atggttttgt agccctgtcg ggcgcgctga tggcgcaatt ccaaggattt gccgatgtcc
    21001 agatgggcat tggcatgatg gtctggggac tggccagtgt gatcatcggc gaggttgtgg
    21061 ttggccggac aagcgtgggg tatgtgattt tcggggcggt tctggggtct gtcctgttcc
    21121 ggctgctggt ggccatagcc ctgcgtcttg gcatgaatcc caacgatctt aagatcatta
    21181 cggcggcatt cgtctttatt gcgctcattt tgcccgggct catgaaacgc aggaaaagtt
    21241 tgtgggggag aaagaaatat gcttgagctc tataatataa aaaaaacctt ccattccgga
    21301 acggtacatg aggtcagggc gctgaatggg gtttccctga cgctggagaa agggagtttt
    21361 gtctgtgtgc tggggagcaa cggatcgggg aaatcgaccc tgctgaacgc gatagccggt
    21421 acttttccgg tagacgaagg gactattcgc ctggatggta cagacatcac tgcctggcca
    21481 gagcacaaaa gggcacgtca catgggacgt gttttccaga atccgctgag cgggacagcc
    21541 cccaacatga ccataagcga aaatatttcg ctggctctca ggcgtggaaa gccccggggt
    21601 cttggcccgg gcatctcgca cggaaagaaa gaggagtaca ggcagatggt ccggccgttg
    21661 aacatggatc tggaagaacg gctggacaat cccataggga agctttccgg aggccagcga
    21721 caggcactca cgcttttgat agcctcggcg ttacagccaa aactgctact gctggatgaa
    21781 catactgcgg ccctggatcc caaaacggct gctcgggtga cggaaatcac ccaaatgatc
    21841 atcaccaggg actcgctgac tacccttatg gtgactcatt ccatgcatca ggccgtacac
    21901 atgggagacc gccttatcat gatgcatcag gggcgtgtgg cctatgattt caaaggggag
    21961 gacaagaaaa ggctcaaggt caatgacctg ctggccctgt ttgacgacct gcgccgcaag
    22021 gatgccattg acccgggagt ggctgcttta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22141 nnnnnnnnnn cctgtttgac gacctgcgcc gcaaggatgc cattgacccg ggagtggctg
    22201 ctttattgag taacaattac gtataatgaa aagcataagt aaaagcctgt tcatcctgtt
    22261 cctggttgcc ggggttttgt tgttgctgga tttgggaaac cgggaaaaag gcaaaaagga
    22321 tgcattgaag atcgcggttt tcaaaatgac cacgcgtccc tcgcttgatg acacagagga
    22381 tggggttttc gaggctttgt cccaaagagg atacatagac ggccagggct gcaccataca
    22441 acgtttttcg gccgacgggg atatggtaac tgccaatgct attgcgcaaa acatagtgag
    22501 cagccggttt gacatggttg tgaccatcag tacgccggca ctgcaggtaa tggccaatgc
    22561 caacaaaaaa ggggacgtgt tgcatgtttt ctgcacggtc accgatccgt atgtttccgg
    22621 agtagggata acaggcactc aaccggacca gcacccgccg caccttgtgg ggataggcac
    22681 attccagccg gtggaagaga cctttaccct gatgaagcag ataaaacccg atctgaaaaa
    22741 ggtgggaacc gtctggtgta ccagcgaaac ctgttccgag gcctgcgtac gcctggcccg
    22801 caagaaatgc gccgaactgg gcattgaact tctggagatg agcgtagaga ataccacgca
    22861 gatcctggag gcggccatgt ccctcacctc acgcggcgtt gaagccttat gggtgggagg
    22921 cgacaacgtg gtagaagcgg ccatagacca gatgatcaat gcggctgcga aagccaggat
    22981 ccctctgatc atcaacaata ccaacagcgt ttacggcaat acattgttcg ggcttggggc
    23041 aaaatatacc gaggtaggct acctggccgg gcagatggct gccgatgtgc tggagggcaa
    23101 gccgacctct gaaataggca tcaggaacat ggtcccgctc catctgctgg tgaatcccga
    23161 tgccctcaat aacattcacg acaaatggaa actcccggag gaattatgaa agtcatttta
    23221 cataactcaa tgagcctgga cggttctttc ataggcattg cagccaacat ggaattgcac
    23281 taccaggcgg tcaattcttt tggtgcgcag gtctacatgg tgggctccaa taccacgtca
    23341 cgggaaagta ttgaacaata cggggaaact gttccaccgg aaacagcatc agattttcag
    23401 aagcccgtgc gcgatccggc cctttcctat tgggtaattc ccgacaccaa agggaccctg
    23461 cagggaatac tgcattatca ccggcggttt gagtattgcc gggatgtgat attactgatt
    23521 tcggatgcaa cccccgccgg gtatatcgag tacctggagc aaaggaatta cggttatatt
    23581 aaaaccggca gtgtgcacgt gaattaccgg gaagccttca agcaacttgc ccaacggtac
    23641 ggtgtggaca ccattcttgt ggatacgggc agccgcctgg ggaatgtact gctcaacgag
    23701 ggcctggtag atgagataag cctggttgtc accccggaaa tcctggcaaa aaacaacaga
    23761 catttatttg aaggcgtgga gcatcacatc aggttgcgca gcaagaaatg cgagacaatg
    23821 cctgacggct atatctggat cgtattcgag atcattggtc actaagccag accacttttt
    23881 gcccgaaaag gatccagtat catggataca gccaaatcaa aaagccgtta agcatgactg
    23941 ctcgaacggc ttaaatattc attatgtgcc gcaaaggtaa gtcaggtatt tcaacataac
    24001 aaataaatcg aaaaaaactt tatcaacaaa gttttaacta ttccagtgtt ttctgcatcc
    24061 agactatatc caggggtttt ccctttttgg taatgatctt ctggaaacgg ccacattcca
    24121 cgaacccgtt tttcttgtgg aaatcaatac tccgggtgtt tatggaggaa atgttggcca
    24181 gaatggtctt gatgcctact ttcagggcat cctcctcaag tcttttcagc gccatggatc
    24241 caatcccttt tcctgtatgg tggtggtcaa tgaaatagga tacttccgca cattccttga
    24301 aggtggggaa aggattatag gcgcgtagca tgcaaaaacc gacaatcttg ctgttttcct
    24361 tgattacgta ggcagggtag cactttgtca tatccaggaa tttttcatag tactcataag
    24421 gcagtacatc atcaggatac gttgccaggc tgttgctcac gtaataatta aaaatgtcca
    24481 tcacagcatc cctgtgctca aaagacatga tttcaaagtt gattttcata gtcatatgtt
    24541 ttacttgccc gcagggccgt tatattctgt tttgccaaaa cccaggatgg ggaaaaagat
    24601 gaacgggagc agcaacaatc cgatggtgaa atccacccct ttgccaaagc tttttgagag
    24661 gaggttatag ccccaaatca aaaagatcag atttacaaat gggataaaca gcagtattac
    24721 ccaccaccag ggtttcccta tgatcttcag ggcaaccagg taatcataaa tgggtattag
    24781 acatgcccat ccaggttttc cggctttggt gaacacatgc cagatggcaa cgacctgtag
    24841 tgcctggata tacataccga agaccataca catgataatg aacggtaaaa atggaagaaa
    24901 gccgccgttg gagaaatcac aagttttcag attttcaaaa aaacattcca tagtttagct
    24961 ttttaagtga tgagtccact ttaaaaagtc ggtgacattc attttgttgt tttacattcg
    25021 tttt
//
LOCUS       DAOSOF010000008        23806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2148_length_23806_cov_5.084249, whole genome shotgun sequence.
ACCESSION   DAOSOF010000008 DAOSOF010000000
VERSION     DAOSOF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_23806_cov_5.084249"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PLM68_00960"
     CDS             complement(<1..1408)
                     /locus_tag="PLM68_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT53974.1"
                     /translation="MKIKKVRISNYRSIKDTIEIDFSQVTALIGPNNSGKSNILWCIN
                     KILGQETSLPGIFTEKDVYRQDPKRNIDVEIEFDQPFEYVPFEGSEPAQIHKIRFIYK
                     LNESGPDTGKRTLNKYCLTKDDRIVQVLSDGNGMGGSQLFTQLQCPLEKIREKIPVVY
                     IRTDRITTHSRSDIRQTLLNGLLKKINEDFMREDNTITIMNADGTETQIPRKVWFEQC
                     IQEAMITLRTGAFSNLKETIRESMLDYLGHGANKEDNSWVIFFNPVIQQNFYQSLEMC
                     LCEGDRLTPVRALGEGVQNAIVLSILNAYNQQKSEGFIFMIEEPELYLDPRMKRNLYK
                     ALTNMGKKNQVIYITHSPQFVSLQDFDSVRIVTNNGDGTRVKAPLGDNIPQIKEQFRS
                     MIPGSELNEIFFTGKVLLVENEKLKKILLDHEARMGLDYDTMNVAILAVGDKSIMPAM
                     VGLAKAFDIRVALAHEQFS"
     gene            complement(1421..2662)
                     /locus_tag="PLM68_00965"
     CDS             complement(1421..2662)
                     /locus_tag="PLM68_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOT53975.1"
                     /translation="MVKIKPFAALRPPRKFVEEVAARPYDVLNSEEAKAEAGEKSLLH
                     ITKPEIDFHPFVDEHSKEAYDKAVENFKLWQQRGWLIRDPKEMYYVYAQTMEGRTQYG
                     LVVCAHTEDYTEGRIKKHELTRKDKEEDRMIHIRIQSANIEPVFFTYHDVPEINRIVE
                     KIVSATPEYDFVAPDGFGHHFWPVSDDADIQRLTTLFASVPAFYVADGHHRTAAAALV
                     GAGRKDANPNHTGEEEYNYFMTVVFPESHLKIIDYNRVVKDLNGMAPQTFLQALAKDF
                     IVECLCDNTIPYVPQRPHNFSMYLDGCWYSLTARQGTYDDSDPIGTLDVTVLSNLIFD
                     RLLDIKDLRTSKRIDFVGGIRGLAELKRRVDSGEMAAAFALYPVTMKQLITIADTGNI
                     MPPKTTWFEPKLRSGLAIHTF"
     gene            complement(2691..3611)
                     /locus_tag="PLM68_00970"
     CDS             complement(2691..3611)
                     /locus_tag="PLM68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOT53976.1"
                     /translation="MKVLVATEKPFAKKAVDGIRAIVEQAGMKLSLLEKYTRKEELLE
                     AVADADALIVRSDKVTREVIQAAPRLRIVVRAGAGYDNLDLAACSEKQVVAMNTPGQN
                     SNAVAELAIGLMIFMARNQFQPGTGTELRGKTLGVHAYGNVGRLVAGLAKGFGMEIYA
                     YDPYIPASVMQSEGVHVAATLEELYRRSDYLSLHIPANKETTGSIGQALLSLMPKSAC
                     LINTARKEVINEPELEAILAQRTDLKYATDVAPGNMDALKAQFDSRVFATAKKMGAET
                     QEANINAGLAAARQIVGYLQKGDKTFCLNK"
     gene            complement(3618..4691)
                     /gene="serC"
                     /locus_tag="PLM68_00975"
     CDS             complement(3618..4691)
                     /gene="serC"
                     /locus_tag="PLM68_00975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOT53977.1"
                     /translation="MKTTHNFNAGPCVLPKEAVQAGIEALKDFNNSGMSVIEISHRSK
                     DWEAVMNECRALWKELLHIPDNYEVLFLGGGASLQFCMVPMNLLEKKAAYLDTGVWAK
                     KALKEAKAFGEVVNVASSAESNYNFVPKKYTIPADADYFHITTNNTIYGTEIHTDLDS
                     PVLLVADMSSDIMSRPVDVSKYGLIYGGAQKNVGPAGVTFVIIRKDILGKVSRHLPSM
                     LDYRIHIEGESMYNTPPVFSIFIMTRTLKWLKNLGGLKAIQKINREKADLLYSEIDRN
                     SLFRGTAAKEDRSLMNICFVMAEGHESLEAEFLDFAKSRGMVGIKGHRSVGGFRASVY
                     NACPKESVQALVDCMREFEKNHK"
     gene            complement(4759..5229)
                     /gene="pyrI"
                     /locus_tag="PLM68_00980"
     CDS             complement(4759..5229)
                     /gene="pyrI"
                     /locus_tag="PLM68_00980"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOT53978.1"
                     /translation="METKKKLKVSAIKNGTVIDRIPSGELFHVIEILGLSRGGFQMTF
                     GTNLESKVLGTKAIIKITDKFFEEDDVNRIALVAPDAVFNIIKDYMVVEKRRVVIPPE
                     ICGLVRCLNPRCITNHEPVKTRFSTSMGPGGIVLTCHYCEKETTQQNLQIISGN"
     gene            complement(5240..6139)
                     /gene="pyrB"
                     /locus_tag="PLM68_00985"
     CDS             complement(5240..6139)
                     /gene="pyrB"
                     /locus_tag="PLM68_00985"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOT53979.1"
                     /translation="MKSLISINDFNKEEMLHVLDLAEKYEAEPNCPIFNDKVIACIFF
                     EPSTRTRLSFEAAASRLGARVIGFASLAATSIAKGESLKDTIKMVSNYADMIVMRHHL
                     DGAARYASEIAGCPVINAGDGANQHPTQTLLDMYSIRKTQGTLENMSITMVGDLKYGR
                     TVHSLLQAMSHFNPRFHFVSCPELAIPAEYRHFLDSRNIPYTEHHTLEGQISNTDILY
                     MTRIQQERFQDPMEYERVKNLMSLDASMLTDTKPNLRILHPLPRIKEIATNVDDRPEA
                     YYFEQARNGVFTRMGIFTYLMNQ"
     gene            complement(6165..6752)
                     /locus_tag="PLM68_00990"
     CDS             complement(6165..6752)
                     /locus_tag="PLM68_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOT53980.1"
                     /translation="MMSPGQLYKTLEAPCEGLYKEKGSRFLSFGFPVSTEEEIRRQLG
                     LMKKKYFDARHICYAYRLGPEGEPWRANDNGEPASTAGKPILGQLISFELSDVLMVVV
                     RYFGGIKLGTGGLINAYRAAARDALAHAAIVEKRVLETVTLQFSYDRTDKVMKAVHDT
                     QAVIVEQSFQEGPLPCRLVVKVPVPGSHVLLSSLE"
     gene            6801..7826
                     /locus_tag="PLM68_00995"
     CDS             6801..7826
                     /locus_tag="PLM68_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53981.1"
                     /translation="MGFALKFTEQAAAALDAFDSALITKTVEENSWFIPEFVRHRLCT
                     LRESLDKEKHLMDGLMDLKIQKNAPRLLSIVAAGNIPLVCWHDFVCALAYAASHPEEV
                     VLEVKLSSKDRVLLPSIVDRMLTQKDSCLAGVKVRFVEKVDPHTQAILFTGGTQAEWF
                     YRRQFPATPMLLRTSRSSLAMLEGNETGFQIDGLVRDCFLYFGLGCRNVTLLLVPRSY
                     DFSEFIDRAGYVFGSLLQAHQGYRNAFRHARACLLMSGSVDVSPVPAFFVLKESELLK
                     PPMAVLHYQYYEDRAGAVRFMEQNKDFIQCVAGTPCTPFGQTQFPSFTDFADGLNTLE
                     WLQRLSS"
     gene            7802..8683
                     /gene="miaA"
                     /locus_tag="PLM68_01000"
     CDS             7802..8683
                     /gene="miaA"
                     /locus_tag="PLM68_01000"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT53982.1"
                     /translation="MATKIIILTGPTGVGKTEQGLAIARLLKTPVINCDSRQIYKELR
                     IGVARPSPEELSQATHYFIATHSIHQPYSAGDYEREAWELVMRLAPFHDYLLMVGGSG
                     LYIDAFIHGIDQMPLPDATLRKELMDMAEEPQGLEELRSRLRILDPVTYARIDLANKR
                     RVMRAVEVCLLSGKPYHSFLTHIPKKRPFEIEVLLMEKPRKELYDRIDKRVFHMMEEG
                     LLEEARNLLPYRQLPALRTVGYRELFGHFEGKYPLEEAISLICRNTRRYAKRQLTYWR
                     DVPRKEDHPLLRPDPTW"
     gene            complement(8637..8999)
                     /locus_tag="PLM68_01005"
     CDS             complement(8637..8999)
                     /locus_tag="PLM68_01005"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOT53983.1"
                     /translation="MEIRGTLIEKLNPIKGTGARGEWVKQEFILETRESSYARKVCIS
                     VFGEDKVRELDGFSIGEIVKASVNIESREYNKRWYTEIRAWRLEKETAVTGVSEDAPS
                     HAGPTMLDQDEGADDLPF"
     gene            complement(9008..9715)
                     /locus_tag="PLM68_01010"
     CDS             complement(9008..9715)
                     /locus_tag="PLM68_01010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT53984.1"
                     /translation="MKTLKILSIALLGAIISISCDSTAPKGDSDFSKGAVDSVSRSIG
                     YSLASMVKQSNFGALNNNEVIKAYKELMAREDEPTQEEMMSWNNDITSYMQRKSVVEG
                     ERNLREGEAFLDENKTKEGIVVLPSGLQYKIIREGNEIRAKATDTVEVHYLGTLIDGT
                     EFDSSYSRNEMAKFPLDRVIPAWTEGMQLVGEGGKIILYVPSGLGYGPNGAGGVIGPN
                     STLIFEVEMNKVFPAVN"
     gene            complement(9771..10805)
                     /locus_tag="PLM68_01015"
     CDS             complement(9771..10805)
                     /locus_tag="PLM68_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HOT53985.1"
                     /translation="MKKFNPDQAGAANGNYFALPFTPKEADIVLVSVPWDVTTSYRPG
                     THRGPEAIIDASAQIDLFNARVPKAWNIPIGTIAVHDELPVLNRSARTMAETIIHCWE
                     AGDDPMESGLGDMLEQVNQACARMNRIVYLQCSEQLRMGKMVGIVGGEHSVPLGYLKA
                     LDEHYGSFGILHIDAHADLREAYEGFTYSHASIMFNILKTISSVASLTQVGVRDYSGQ
                     ENQIICNDARIAFFPDHALHEDMYEGLTWREQCHRILESLPDQVYISLDIDGLSPELC
                     PNTGTPVPGGLGFQQVDYLLYLLASSRRRIIGFDLCEVAPSGDNPWDAIVGARMLHKI
                     CCYTYLNNNK"
     gene            complement(10802..11773)
                     /locus_tag="PLM68_01020"
     CDS             complement(10802..11773)
                     /locus_tag="PLM68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOT53986.1"
                     /translation="MNTPDRDIKQTALDVIEKEQFAIGGLRNSIDQSFLQVVQILHAS
                     TGRLIVTGIGKSAIICQKIAATLNSTGTPAVFMHAADAIHGDLGIIQPGDVVMCLSKS
                     GDTAEIKVLVPLIRKMGNLLIGMVGNRDSYLGRSADYVLLASVSSEACPNNLAPTAST
                     TAQMVLGDTLAVCLLKLRGFTPSDFARIHPGGSLGKKLYLRVADIMDPALRPMVRPGQ
                     SVRDTIFVISRNRLGATAVLDEGENLLGIITDGDVRRMVEKNDSLENLCASDIMTKNP
                     KTIAEGELAVHAYRMMEASKITQLIVLRKNRYMGMVHIHDIIREGIV"
     gene            11829..14018
                     /gene="recQ"
                     /locus_tag="PLM68_01025"
     CDS             11829..14018
                     /gene="recQ"
                     /locus_tag="PLM68_01025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOT53987.1"
                     /translation="MHNSSELHIQLKRHFGFNTFKGQQEEIISHLLQGGDAFVLMPTG
                     GGKSMCYQLPALLMEGTAIVISPLIALMKNQVDVLRGFSEDNYGVAHFLNSSLTKTQV
                     AEVRESLLSGRTKLLYVAPESLTKDENIQLFKQLKISFYAIDEAHCISEWGHDFRPEY
                     RRIRKLVNEIGKAPVIALTATATPKVQRDIQKNLDMMDAKVFKTSFNRENLYYEVRPK
                     VNVNKELVKFIRQNHGKSGIVYCLSRKKVEEIASFLQVNDIRALPYHAGMDAAARTIN
                     QDLFLMEEAEVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGQC
                     IAFYSYKDIQKLEKFKQGKPIAEQEISKQLLMETMAYAETNLCRRKVLLNYFGEDYEQ
                     DNCECCDNCLHPKKQFEGQEEMCLVLETVQKSLQQFNAEHVANIIAGVSNAMIKSYQH
                     HKLDVYGMGKDKTPNFWNAIIRQAIVLRFLDKDIEKYGALSVTPAGRDFLENPTTLML
                     YEDRQMEEEEDSEIEAAAKAGGGGGDIELLAMLKDLRKDMAHRLKLAPFVLFQDVSLE
                     DMSISYPITLEELARCQGVGEGKARKYGKPFIELIEKYVEENEIDRPQDIVVKSVINK
                     SQNKVYIIQSIDRKLDFVDIAEAKGLTIDELLEEIEVIINSGTHLDIDYYLRQNIDDD
                     KLETIYDYFRYDAKSESLSEAVKELGPEYTEEEVRLVRIKFLVEVAN"
     gene            14038..16308
                     /locus_tag="PLM68_01030"
     CDS             14038..16308
                     /locus_tag="PLM68_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53988.1"
                     /translation="MRKRTIIFLVLLLLLLAGASVYGFFLLFRDSGDHAAGNSTYRAV
                     PVDAVLVQHFSGLNTLTGEVLTPGSYLESLFHPEQETQAFLAELTAFLTAAYPGMAQA
                     ETLCSLHPSAKNTLAALFCISGEKAQDPQWWDNFLGKSLLSFKTRSYNGQTIYQAGGE
                     ESDHSVYASCVNDLLMVSSSLVVLESSLRHLGNGSSLADDPSFARLVEQTPVSKPTRI
                     FIPHQGIPSMFAAYLGMPLQKYAAFVRSAARWTVLDGFISGHEVKMDGYSLFPSGAEH
                     YFATFLGQEPQKLMAPQILPAAAVAGISIGISDLASYLEGYGRYLELQKSNRKGPDKE
                     RLEWFSLLYPTEVSLSCVPFGGELQWIAVIHSRYIHQARIQFALLNRQEENKVMENPV
                     PGLLPEIFGPVFTLCQASDYCYIGPFILFGPRKLLNGILKSNLDGNYHSLAAAISQTD
                     ASPGVMNASNLTLFLQPTAGLKQIQTLMDKRYAERLDAWGRYNAQYTFLQFSSLEDRL
                     YAHVSVFGDSLETSPLIPRQPEARIRSGMHQDTLARGRPPFKVFNHFTKKDNELFQTP
                     WPECRLIMRDHTGKTLWEKTMEGPIQDGVRQLDLLKNDKLQMLFSIGNRLYLLDRLGR
                     NVSPYPKAYASSILYGPFVFDPEGKKEYRILLAHQDNVLRCYDKFGNVPEGWSDFTLP
                     GYLTGQPRHTRWENTGLWIVYTEAGTVILSESGQVRGMTTRKECLDPQAEVRISGPYE
                     LYGTTIEGKTLAIMLE"
     gene            16314..16517
                     /locus_tag="PLM68_01035"
     CDS             16314..16517
                     /locus_tag="PLM68_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT53989.1"
                     /translation="MSLLTIIFFIIVAGYFFINLIPLMLSRKFRKMQEQGRKRDNIEN
                     NTSRKKRIDKGAGEYVDYEEIDE"
     gene            16514..18349
                     /locus_tag="PLM68_01040"
     CDS             16514..18349
                     /locus_tag="PLM68_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT53990.1"
                     /translation="MMKSFMRILAYGKPYSRYWPSYLVLSVFSVFFGIANYALVAPLL
                     TVLFQADTLPGEMARPVFSLSIPYFVDLFRYYMVMIKAKDGILMALVYVSVSLLVASF
                     LANLTYYLSQRILVDMRANLMKNIRNDLFSKISRLHIGYFHDQRKGDLLSTVSNDVNE
                     VQNSVAGSFHIIFRDPLLIAGFLSMLFYMSPKLTLASLLTLPLSALLIGRITRSLRSG
                     AEVSQSLLGKLLSHFEEAISGSRIVKAFNAQGYILDRFDGVNDQHRMATRRLYNRQEL
                     ASPLGEFLGISVAAAVLFYGGWLQVRGDLGMTWPEFVVYIGFYWRVLEPAKSITRTYA
                     VIRKGMVSALRIFSILDAPVEIRKDQDAMPVKTFSERIEYRNVSFRYKETDPYVLRDI
                     NLVIPKGKVIALVGPSGAGKTTLADMLPRFYDVTEGSVLLDGIDIRKYRPRDLIGLMG
                     IVTQESILFNDTVANNITFGLVNISRPEVEHAARVANAHDFIMELEHGYETNIGDRGE
                     KLSGGQRQRLAIARAVFKNPPILILDEATSALDSESERMVQEALTKLMKNRTSVVIAH
                     RLSTVRDADEIVVIDKGRITERGTHSRLLEQNGMYRHLYDLQAFG"
     gene            18368..20038
                     /locus_tag="PLM68_01045"
     CDS             18368..20038
                     /locus_tag="PLM68_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT53991.1"
                     /translation="MSKDILKTESLNELFENSFKKYWDYKALTEFNGQTLYYSDLARR
                     IRMIHIGFEKCGLKKGDKVALCGKNQSNWAVAFLATISYGAVVVPILHEFKPGNIQHL
                     VNHSDARIFFVGEQIWEGLNEAEMPNLEAIVELNDFSILYTPDKEIINIRKGLEKNFT
                     ELYPEGMKPEDVRFHRDQPEELALINYTSGTTGFSKGVMLPYRSLLSNVLFAWEVLPQ
                     VSNGARVVSMLPSAHMYGLMFELLFEMTSGSHVHFLTRMPSPKVIVEAFQSVKPRVII
                     AVPMIIEKIYKQKLLPLLKRPSIKVMLRMPFIDQRIKNRIHDELVKAFGGEFVEIIVG
                     GAPLNHEVEPFLKSISLAFTVGYGLTECGPIVSYAPWNERPLFSCGRAAPRMSIRIDS
                     EDPETVPGEIQLKGDNVMVGYYKNPEATEQVFTEDGWFRTGDMGTMDKDGYLYIKGRS
                     KTIILGPNGQNIYPEEIESSINNMSYVNESLVIMEDNKLVALVYPDFEKAAAEGLPDS
                     AMDATMEAMRAQINLDLPKYCQIARVRIVPEEFEKTPKKSIKRFLYNG"
     gene            20031..20441
                     /locus_tag="PLM68_01050"
     CDS             20031..20441
                     /locus_tag="PLM68_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOT53992.1"
                     /translation="MDKQTQFDLHYLEMARIWSQNSYCERRKVGALMVKDRMIISDGY
                     NGTPRGFENVCEDEEGHTKSYVLHAEANAITKVARSNNSSEGATIYITASPCIECAKL
                     IIQSGIVRVVYSDDYRLQEGIELLKRAGIQVDKF"
     gene            20448..22124
                     /locus_tag="PLM68_01055"
     CDS             20448..22124
                     /locus_tag="PLM68_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT53993.1"
                     /translation="MEGFRRLRKTLGIVLVILVALAITGIIRWDIVRKQNGGGRKQLG
                     REWSKLELILEQIQNNYVDPVDVSAFIEKTLPTIMEELDPHSVYLSPDELRTADEELR
                     GNIEGIGIVFNVPSDTATVINVVVGGPSEKMGLQPGDKIIKIDTQIVAGVQMDQDSIV
                     RLLKGPKGTQVDVHVKRYGENDLIHFSIVRDKIPVNSVDVAYMADSLTGYIKLSRFSM
                     TSYAEVLGAIADLMKQGMKQVIFDLRDNSGGYLDQALKITGEFLDENKLVVYTEGEHR
                     ERQDFYTKKRGFCYDMPLAVLINEASASSSEIMAGALQDNDRGTIIGRRSYGKGLVQE
                     PIYFSDNSGLRLTVARFYTPLGRSIQKPYMSNSREYAYDLLERYKHGELMQEDSIPKN
                     DSLRFVTQAGKVLYGGGGIIPDVFVPLDTTGVNDLYLRISRQNLPIRFSMDFADRNRV
                     QLSGIDTYDDLEQFFASTQLEEEFLRYLNKQGIHPLPVQWKECGGILLTQIQAYIGRN
                     TRLEDKGFYPYLGRIDNVLIKALEVLGTPLITEPAEPGLVFAPMGNNLDE"
     gene            complement(22059..22940)
                     /gene="era"
                     /locus_tag="PLM68_01060"
     CDS             complement(22059..22940)
                     /gene="era"
                     /locus_tag="PLM68_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT53994.1"
                     /translation="MSATHKAGFVNIIGNPNVGKSTLMNALVGEKLSIITSKAQTTRH
                     RIMGIVNGPDYQIVYSDTPGIIKPYYLLQENMMEYVESALADADILLYVTDVVEKLAK
                     NPDYIEKLNRIEVPVLVVLNKIDKSSQAEVLSLMDQWHTMVPKAEIFPVSALEDFNLD
                     SLLERILALLPASPPWFDKDQLTDKSLRFFASEIIREKIFLNYSKEIPYCMEVTVEEF
                     LESQARYDIRAVISVMRDSQKGIIIGDKGSALKRVGTQARLDMEDFFQKKVFLELFVK
                     VVPHWRENKTRLRRFGY"
     gene            complement(22942..>23806)
                     /locus_tag="PLM68_01065"
     CDS             complement(22942..>23806)
                     /locus_tag="PLM68_01065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT53995.1"
                     /translation="SERPRREGGYSSERPRREKSYRSERTYKSFGHKPAPKKTEAPQP
                     KQPKVPGEVRLNKYIANSGLCSRREADTFITTGLVTVNGKVVDTLGVKVTPADDVRVN
                     GQRIKGEPKTYIVMNKPRGYVTTLSDDHAEKSVMDLIAGKCEQRVFPVGRLDKMTMGV
                     LLFTNDGELTEKLTHPSYNRKKIYQTRLDMNLKQSDMEKLVNGIELEDGMMHVDQIAY
                     IDNDEKEIGLEIHSGKNRVVRRLFEALGYKVKKLDRVYFAGLTKKNLKRGQWRFLTEE
                     EVNMLITGFYQ"
BASE COUNT         5882 a         5688 c         5989 g         6247 t
ORIGIN      
        1 cactgaactg ctcgtgtgcc agggcaacac ggatgtcaaa ggcttttgcc agcccgacca
       61 tagccggcat gatagacttg tctcccacag ccaggatggc cacgttcatc gtatcataat
      121 ccagacccat acgggcttca tgatctaaca gaatcttctt tagcttttcg ttttccacca
      181 gcaacacttt gccggtgaag aatatttcgt tcagttcgct tcccggaatc atgctcctga
      241 attgttcctt tatttgtggg atgttgtcac ccagcggggc tttcacacgc gttccgtcac
      301 cattgtttgt gacaatcctg actgagtcaa aatcctgcag ggaaacaaat tggggagaat
      361 gggttatata gatcacctgg tttttcttac ccatattagt caaagccttg taaaggttgc
      421 gtttcatacg cgggtccagg tacagttcgg gttcttctat cataaaaatg aaaccctcgc
      481 ttttttgctg gttataggcg ttcagtatgg acaggacaat ggcattttga accccttccc
      541 ccaaggccct gaccggggtg agacgatctc cttcgcaaag gcacatctcc agcgactggt
      601 aaaaattctg ctgaatgact ggattaaaaa agattaccca gctgttgtcc tctttatttg
      661 caccatgacc caggtaatcc agcatggatt cgcggatggt ttccttgaga ttggaaaaag
      721 caccggtccg cagggtgatc atggcttcct ggatgcattg ttcaaaccac accttgcggg
      781 ggatctgtgt ttccgtcccg tcagcattca taatggtgat ggtattgtct tccctcatga
      841 aatcctcgtt gatctttttg agcaggccgt taagcaatgt ctgacggata tcggagcggg
      901 aatgggtggt gatcctgtct gttcggatat aaaccaccgg gatcttttca cggatttttt
      961 ccaaggggca ttgaagctgt gtaaaaagtt gggaaccgcc cattccgtta ccatcggaca
     1021 ggacctgcac tatgcggtca tcttttgtga ggcagtattt gttcagggtg cgtttgcctg
     1081 tatcgggccc gctttcattc agtttatata tgaaacggat cttgtggatt tgggctggtt
     1141 cacttccttc aaacggaacg tattcaaaag gctggtcgaa ttctatctcc acatcgatat
     1201 ttcttttcgg atcctgacgg tatacgtctt tttcggtaaa gatccccggt aaggaagttt
     1261 cctgtcccag gatcttgttg atgcaccata aaatgttgct tttaccagaa ttattgggtc
     1321 ctatcagggc cgttacctgt gagaaatcaa tttcaatggt gtcctttatg gaccggtaat
     1381 tagatatacg taccttttta attttcattg ccagatgata tcagaatgtg tgtatggcga
     1441 ggccgctccg gagcttgggc tcgaaccatg ttgtctttgg gggcattatg ttacctgtgt
     1501 cggcaatggt gatcagttgc ttcatggtaa cgggatacag tgcaaaagcg gcggccattt
     1561 ctcctgagtc cacgcgacgt ttcaattctg ccaggccgcg tattccacct acgaagtcta
     1621 tgcgtttgct ggtcctcagg tctttgatgt ccagcaatct gtcaaaaata aggttggata
     1681 ggacagtaac gtccagggta ccaatgggat cgctgtcatc ataagtaccc tgacgcgcgg
     1741 tcagggaata ccagcaacca tccaggtaca tggagaaatt atgcgggcgt tggggaacat
     1801 acggtatggt attgtcgcac aggcattcca ctatgaaatc ttttgccagc gcctggagga
     1861 aggtctgggg tgccataccg ttcaggtctt tcacaacacg gttgtaatca atgatcttca
     1921 ggtggctttc cggaaatacg accgtcatga agtagttgta ttcttcttct cccgtgtggt
     1981 ttgggttagc gtccttgcgc ccggcgccta ccagggcagc tgcagcggtg cggtggtggc
     2041 catcggccac gtagaaggcc ggaacagagg caaaaagggt ggtgagacgc tgaatgtctg
     2101 catcgtctga aacgggccag aaatgatgtc cgaatccatc cggtgccaca aagtcatatt
     2161 cgggtgttgc agaaacgatc ttttccacga tccggttgat ttccggaaca tcatgatacg
     2221 tgaaaaagac cggctctata ttagcgctct ggatccgtat atggatcatc cggtcttctt
     2281 ccttgtcctt gcgtgtgagt tcgtgtttct ttatccttcc ctccgtgtaa tcttcagtat
     2341 gggcacatac caccagaccg tactgggtgc ggccttccat ggtctgcgca tagacgtagt
     2401 acatttcttt cgggtccctg atcagccatc cccgttgctg ccacagcttg aaattctcaa
     2461 cggctttgtc gtatgcctct ttggaatgct cgtcaacaaa gggatggaaa tcgatttcag
     2521 gttttgtgat atgaagcaat gacttttctc cggcttcggc tttggcctct tcagaattca
     2581 acacatcata aggacgggct gccacttctt ccacaaattt cctggggggt ctgagggcag
     2641 caaaaggttt tatctttacc atatatgcct gtcttttgct ttggtacgtt ctatttgttc
     2701 aggcaaaagg ttttatcccc tttctgaagg taacccacta tctggcgtgc agcggccagt
     2761 ccagcattga tgttggcttc ctgggtttcc gctcccatct tttttgcagt cgcaaatacg
     2821 cgggaatcga attgtgcttt caaggcatcc atattgccgg gtgccacatc tgttgcatat
     2881 ttcaggtctg tgcgttgggc cagaatggct tccagttcgg gttcattgat cacttccttg
     2941 cgggctgtgt ttataaggca ggcgcttttt ggcatgagcg aaagcagcgc ttgtcctatg
     3001 gatcccgttg tttccttatt ggcagggatg tgcagcgaga ggtagtccga acgcctgtag
     3061 agttcttcca gggtggcggc cacatggact ccttccgatt gcattacaga ggcgggaata
     3121 tatggatcgt aggcatagat ttccattcca aaacctttgg ccaggccggc aacaaggcgt
     3181 ccaacattcc cgtaagcgtg aacgcccagg gtcttgcctc ttaactctgt accggtaccg
     3241 ggctggaatt ggttacgggc catgaagatc atcaagccaa tggccagttc ggccacggca
     3301 ttggagtttt gtcccggagt gttcatggcc acaacctgtt tttctgagca ggcggccagg
     3361 tcaaggttgt cgtaaccggc accggcacgt accacgatcc gcagccgcgg cgctgcctgg
     3421 ataacttcgc gggttacttt gtctgaacgc actatcaggg cgtctgcatc ggcaactgct
     3481 tccaggagtt cttctttccg ggtgtatttt tccagcaagg acaatttcat ccctgcctgt
     3541 tccacaatag cgcggatacc gtcaacggct ttctttgcaa atggcttttc tgttgctacc
     3601 agtactttca tatcgactta tttatggttt ttctcaaatt cgcgcataca atcaacaagg
     3661 gcctgcacac tttcttttgg acaggcgttg tacacagagg cgcggaatcc gcctaccgaa
     3721 cggtggcctt taatacccac catgccgcgg gattttgcaa aatcaaggaa ttcagcttcc
     3781 agggattcat gcccctcggc catgacaaaa caaatattca tcagggagcg gtcttctttc
     3841 gctgcagtac ctctgaaaag ggaattacgg tcaatctcgg agtacagcaa gtctgccttt
     3901 tcacggttga ttttctggat agctttcagc ccgcccaggt tcttcagcca tttaagtgtc
     3961 cgggtcatga taaaaatgga aaataccggt ggcgtattgt acatggattc accctctatg
     4021 tggatcctgt aatccagcat ggagggaaga tgacgtgaaa ccttccccag gatgtctttg
     4081 cggatgatca cgaaagtcac cccggccggg cccacgttct tctgggcgcc accgtatatc
     4141 aggccatatt ttgaaacatc aaccgggcgg ctcatgatgt ccgaggacat gtcggcaacc
     4201 agtagaacgg ggctgtccag gtcggtatgt atttctgtcc cgtagatggt gttgttggtg
     4261 gtgatgtgga aataatcggc atcagcgggg atggtatatt ttttgggaac aaaattatag
     4321 ttgctttcgg ccgaggaagc cacgttgacc acttccccga aggcttttgc ttctttcagg
     4381 gctttcttgg cccagacgcc tgtatcaaga taggcagcct ttttttccag caggttcatg
     4441 gggaccatac agaattgcag gctggccccg ccgcccagga acagcacttc atagttgtcg
     4501 ggaatatgta ataattcttt ccacaaagcg cggcattcat tcatcaccgc ttcccaatcc
     4561 ttggaacggt gagatatttc gattacggac attccggaat tgttaaaatc cttcagggct
     4621 tcaatgccgg cttgaacagc ttctttaggc aggacacagg ggcctgcatt aaaattgtga
     4681 gtcgttttca tattgaatca ggtttagtaa caaattattc gtgtatgcaa atataaagta
     4741 aatatgtaca atcagatatt aattaccgct aatgatttgc aagttttgct gcgtggtttc
     4801 cttttcacag tagtgacagg tcaggacgat gccgccgggg cccatactgg tggaaaaacg
     4861 ggtcttgacc ggttcatgat tggtgatgca cctgggattc aggcacctta ccaggccgca
     4921 gatctcggga ggtatgacaa cgcgtctttt ttccaccacc atgtaatcct tgatgatgtt
     4981 gaagacggca tcgggagcta ccagtgctat ccggttaacg tcgtcttcct cgaaaaactt
     5041 gtctgttatc ttaataatgg cttttgtccc cagcacctta ctctccaggt ttgtcccgaa
     5101 ggtcatctga aaacctcccc tggacagccc cagtatctca attacatgga aaagttcccc
     5161 ggaagggatc cggtctatga cagttccgtt cttaatggca cttaccttga gttttttctt
     5221 tgtttccatg atggtggttt tattggttca tcagataagt gaaaatgccc atgcgggtaa
     5281 acaccccgtt gcgggcctgt tcgaaataat aggcttcagg cctgtcgtcc acattggtgg
     5341 cgatttcctt tatcctgggc agggggtgca ggatacgcag gttaggcttg gtatctgtga
     5401 gcatggaggc atccaggctc atcaggttct tgacccgttc gtattccatg ggatcctgga
     5461 aacgctcctg ctggatcctg gtcatgtaca ggatatctgt attgctgatc tgtccttcca
     5521 gggtatggtg ttccgtatag ggtatgttcc tggaatccag gaagtgcctg tactctgccg
     5581 gtatggccag ttcggggcag gatacaaaat gaaagcgtgg attgaaatga ctcatggcct
     5641 gcagaagcga gtggacagtc ctgccgtatt tcaggtcgcc caccatggtg atggacatat
     5701 tctccagtgt cccctgtgtt ttccgtatgg agtacatatc gagcaatgtt tgcgtggggt
     5761 gctgattggc tccgtctccg gcgttgatca ccgggcatcc ggcgatttcc gaagcatacc
     5821 gggctgctcc gtccaggtga tgccgcatga cgatcatatc ggcatagttg gaaaccatct
     5881 ttatagtgtc tttcaggctt tcacccttgg caatgcttgt ggcagccagt gaagcaaaac
     5941 ctatgacccg tgccccaagc cggctggctg ccgcctcaaa actcaaccgg gttcgtgtag
     6001 agggttcaaa gaatatgcag gcgatgacct tatcgttaaa gatcgggcaa ttgggctctg
     6061 cctcgtattt ttcagccagg tcaagcacat gcagcatttc ttctttgtta aaatcgttga
     6121 ttgaaatgag cgatttcata tacgaaaaat tacatttgtt ctgttcattc cagggaagat
     6181 aaaagtacgt gactgcctgg tacagggact tttactacca gacggcaggg aaggggacct
     6241 tcctggaagg attgttctac tataacggcc tgtgtgtcgt gcacagcctt catgaccttg
     6301 tcggtcctgt cataactgaa ttgcagcgtg acggtttcca gcaccctctt ttccacaata
     6361 gcggcatgag ccagggcatc tctggcggct gctctgtagg cattgatcag cccgccggtt
     6421 cccagtttga tacctccgaa ataacgtact acaaccatca acacatctga taattcaaaa
     6481 gagatcagtt gtccgagtat gggtttgccc gccgttgaag caggctcccc gttgtcgttc
     6541 gccctccacg gctccccttc cggccccagg cggtatgcat agcaaatgtg gcgggcatca
     6601 aaatactttt ttttcatgag ccccagctgg cggcgtattt cttcttctgt agaaacggga
     6661 aaaccaaagg aaagaaagcg gcttcccttt tccttgtaaa ggccttcgca gggcgcttcc
     6721 agggttttgt atagttggcc gggagacatc atgcgaaaat acgtcttttg cgtatctttg
     6781 caaaatcgag cctcataatt atgggatttg ccctgaaatt cacggaacaa gctgccgcag
     6841 ccctggatgc gtttgattct gctctcatta ccaagacagt agaagaaaat tcatggttca
     6901 taccggaatt tgtccgacat cgtctgtgca cgctcaggga aagtctggac aaggaaaaac
     6961 acctgatgga cgggcttatg gatctgaaaa tacagaaaaa cgccccgcgg ctcctttcca
     7021 tagtggctgc gggaaacata ccccttgtct gctggcatga ttttgtatgt gccttggcct
     7081 acgctgcatc ccaccctgaa gaggtggtgc tggaagtcaa attgtcttca aaggacaggg
     7141 tattgcttcc ttccattgtg gaccgtatgc tcactcagaa ggattcctgc ctggccggag
     7201 taaaggtgcg ctttgtggaa aaggtggatc ctcacacgca ggccattttg tttaccggcg
     7261 gcacacaggc cgaatggttc tacaggcggc aatttcccgc cactcccatg ttgttgcgta
     7321 ccagccggag ttcactggct atgcttgaag gaaacgaaac gggattccag attgatggtc
     7381 tggtccggga ttgttttctg tattttggtc tgggctgccg caatgtaacg cttttgctgg
     7441 tgccccgttc ctatgatttc agtgagttca ttgaccgggc aggatatgtt ttcggatccc
     7501 tgctacaagc ccaccagggc tacaggaatg cattccgaca tgccagggct tgcctcctga
     7561 tgtcgggaag cgtcgacgtg agcccggtcc ccgcgttctt tgtgcttaag gaaagtgaat
     7621 tgctgaaacc acccatggct gttctgcact accaatatta tgaagaccgt gccggagctg
     7681 tacggtttat ggagcaaaac aaggatttca tccaatgtgt ggccggcact ccttgcacac
     7741 cttttggaca aacacagttc ccatcattca ccgattttgc cgacggacta aacactttgg
     7801 aatggctaca aagattatca tcctgacagg acccaccggt gtgggaaaaa cggaacaggg
     7861 tctggccatc gcccgcttgc tgaaaacccc tgtgatcaat tgtgacagcc gtcagatata
     7921 caaggaactg cgcataggtg tggcacgccc ttcgcccgag gaactttccc aggcaacgca
     7981 ttatttcatt gccacgcatt ccatacacca gccttattcg gcaggagatt atgaaaggga
     8041 agcctgggaa ctggtgatgc gcctggctcc cttccatgac tatttactga tggtaggcgg
     8101 ttccggtttg tatatagatg ccttcatcca cgggatcgat cagatgccgc tgcccgatgc
     8161 caccctgagg aaagaactta tggatatggc agaagagccg cagggcctgg aagaactccg
     8221 ctccaggctc aggatcctgg atcccgtaac atatgcacgg atagacctgg ccaacaaacg
     8281 acgggtcatg cgggcggtag aagtgtgttt gctttcaggg aagccttatc attcttttct
     8341 cacgcacatc cctaaaaaaa ggccctttga aattgaggta ctcctgatgg aaaaaccacg
     8401 caaagagctg tacgatcgta ttgacaaaag ggtattccac atgatggagg aaggtctgct
     8461 ggaagaggcc cgcaatctat taccatacag acagctccct gcactccgga ctgtcgggta
     8521 cagggagctg ttcggtcatt ttgaagggaa ataccctctg gaggaagcaa tcagcctgat
     8581 ttgccgcaat acccgcaggt atgccaaacg acagctgact tactggagag atgtacctag
     8641 aaaggaagat catccgctcc ttcgtcctga tccaacatgg taggaccggc atgtgaggga
     8701 gcatcttccg aaacgcccgt aacggccgtt tccttttcca gtcgccaggc gcgtatttcc
     8761 gtataccagc gcttattgta ttctctcgac tctatattga cagaagcctt cactatttca
     8821 cctatggaaa aaccatccag ttcccggacc ttatcttctc cgaaaacaga aatgcagact
     8881 tttctggcgt aagaagattc acgggtttcc aggataaatt cctgctttac ccattctccc
     8941 cttgccccgg ttcctttgat gggattgagc ttttcaatta aagttcccct gatttccact
     9001 tcagtgttca gtttacggca gggaacacct tgttcatttc cacttcaaag atcagcgtac
     9061 tgttggggcc aataacgccg ccggctccat tggggccgta tccgagtcct gaaggaacat
     9121 agagaatgat ttttcctccc tcaccaacaa gctgcatgcc ttcagtccag gcagggatca
     9181 ccctgtccag ggggaatttg gccatctcgt tgcgcgagta agaagagtcg aattcagtac
     9241 cgtcaatcag ggtgcccaga taatgcactt ctacggtgtc ggttgccttg gcacggattt
     9301 cgtttccttc acggatgatc ttgtattgca gcccgctggg cagcacaact atgccctcct
     9361 tggttttgtt ctcgtccagg aaagcttctc cttccctgag gttgcgttct ccttcaacaa
     9421 cactctttct ttgcatataa gaagtaatgt cgttgttcca ggacatcatt tcttcctggg
     9481 tcggctcatc ctcgcgggcc atcagttcct tatatgcctt aataacctcg ttgttgttca
     9541 atgctccgaa attggattgt tttaccatgg aggccagcga ataaccaata gaccggctta
     9601 cagaatcaac agctcctttg gaaaaatcgg aatccccctt gggtgcggta ctgtcgcagg
     9661 aaatggaaat gattgcgccc aacagggcga ttgataaaat ctttaatgtt ttcattgtaa
     9721 ttatagttaa aaagattgtt cgcctgaata ttaaacaacg aatgtaaggt ttatttattg
     9781 ttatttaaat aggtgtagca gcaaatttta tgcagcatgc gtgcccctac tatggcatcc
     9841 cagggattat cgccggaggg tgccacttcg cacaggtcaa atccaatgat cctgcgccgg
     9901 gaagaagcca gcaggtaaag caggtagtcc acctgctgaa atcccagccc gccgggaaca
     9961 ggggtaccgg tattgggaca aagttccggg gaaagcccat ctatatcaag gcttatgtaa
    10021 acctggtccg gaaggctttc aaggatcctg tgacattgtt ccctccatgt aaggccctcg
    10081 tacatgtctt cgtgaagcgc atggtcggga aaaaatgcta tgcgtgcatc attgcagatt
    10141 atctggtttt cctgacccga ataatcacgg acacccacct gtgtcaggga ggcgactgaa
    10201 gatatggttt tcaggatgtt gaacatgatg gaggcatgtg aataagtaaa tccttcatag
    10261 gcttcacgaa ggtccgcatg tgcatcaata tgcaggatac cgaacgagcc gtagtgttcg
    10321 tccagcgcct ttaggtatcc cagaggcaca gaatgctcgc caccaacgat tcctaccatt
    10381 tttcccatcc ggagttgttc tgagcactgc aggtagacaa tccggttcat ccgggcacaa
    10441 gcctggttga cctgttccag catatctccc agtccgcttt ccatgggatc atctcctgct
    10501 tcccagcagt ggataatcgt ttcggccatc gtgcgtgcgg agcggttcaa tacgggcagt
    10561 tcgtcgtgta ccgcgatagt gccaatggga atattccagg ctttaggcac cctggcattg
    10621 aagaggtcta tctgtgctga agcgtcaatt atggcttcag gccccctgtg tgtccctgga
    10681 cggtacgagg tggtcacatc ccatggtacg gaaaccagta caatatctgc ttcctttggt
    10741 gtgaaaggca atgcgaaata gttgccgttg gctgcacctg cctggtctgg gttaaacttt
    10801 ttcatacgat tccttctctt attatatcgt gtatgtggac cattcccata taacggtttt
    10861 tccgcagcac aatgagctgg gtgatttttg aggcctccat catgcggtaa gcatgcaccg
    10921 ccagttcccc ttccgctatg gttttgggat tcttagtcat gatatccgaa gcacacaggt
    10981 tttccaggct gtcgttcttt tctaccatac ggcggacatc tccgtctgtg attataccca
    11041 gcaggttctc cccttcgtcc agaacggctg tggctcccag ccggttcctg gagatcacaa
    11101 aaatggtatc acggacagat tgtccgggcc tgaccatcgg ccgcagggcc gggtccatga
    11161 tgtcagcaac acggagatac aatttctttc ccagtgatcc tcccggatga atccgagcga
    11221 aatcagaggg tgtgaatcca cgcagtttca acagacatac ggccagggta tctcccagga
    11281 ccatctgtgc agttgtgctg gccgttggag ccaggttgtt agggcatgct tcactggaga
    11341 ccgaggccag taatacgtaa tcggcagaac ggcccaggta ggaatccctg tttccgacca
    11401 tgcctatgag caggttcccc attttgcgta tcagtggcac cagcactttg atttccgctg
    11461 tgtccccgct tttggaaagg cacatcacca catctcccgg ttgtattatg cccaggtcgc
    11521 cgtgtatggc gtcggcagcg tgcatgaata cggcaggcgt tcccgtggaa ttaagtgtgg
    11581 cagcgatctt ctggcagatt attgcgcttt tgcctatacc tgttacaatc agccggcctg
    11641 tggaagcgtg caggatctgc acaacctgga gaaaagactg gtctatggaa ttacgcaggc
    11701 cgcctatggc aaattgttct ttttcaataa catcaagtgc agtttgttta atatcccggt
    11761 caggtgtatt cataaaattt tgaaagaata aaacaaacgt ataacttagt ggacaaaggt
    11821 agtaaaatat gcacaattca tccgaattgc acattcaact gaaaaggcat tttggattca
    11881 atacattcaa gggtcagcag gaagaaatca tctcccattt actccagggt ggagatgcct
    11941 ttgtgctgat gccgaccgga ggcggaaaat ccatgtgtta ccagttgccg gcccttctga
    12001 tggaagggac agcgattgta atatcccctc tcattgccct gatgaaaaac caggtagatg
    12061 tcctgcgcgg tttttcagaa gacaactatg gagtagcaca ttttttaaac tcttcactta
    12121 ccaagacaca ggtggccgag gtcagggaat ctcttctttc agggaggaca aagttgctct
    12181 atgtggcccc tgaatcgctt acaaaggatg aaaacatcca gttgttcaag cagctgaaga
    12241 tctctttcta cgccattgat gaggcgcatt gtatttcaga gtggggtcac gacttcaggc
    12301 ctgaatacag gcggatcagg aagttggtca acgaaattgg caaggcgccc gtgattgccc
    12361 ttacggcaac ggctacaccc aaggtgcagc gggacatcca gaaaaacctg gacatgatgg
    12421 atgcaaaggt tttcaaaaca tccttcaaca gggaaaacct gtattatgag gtcaggccaa
    12481 aggtgaatgt gaataaggaa ctggtgaaat tcatccgcca gaaccatggt aaaagcggca
    12541 ttgtatattg cctgagccgc aaaaaggtgg aagaaatagc ctcgttttta caagttaacg
    12601 acatccgggc actgccctac cacgccggta tggatgcagc tgccaggaca atcaaccagg
    12661 atctcttcct catggaagag gctgaagtca tcgtggccac catcgcattc ggaatgggta
    12721 ttgacaaacc cgatgtccgt ttcgtgatac attacgatat tcccaagtcc ctggaagggt
    12781 attaccagga aacaggccgg gcaggccgtg acggcggaga aggtcaatgc atagcattct
    12841 acagttacaa ggatatccag aagctggaaa aattcaaaca gggaaaaccc atagcagaac
    12901 aagagatcag caagcaactg ctcatggaaa caatggccta tgccgagacc aatctttgca
    12961 ggcgcaaggt gttactcaat tatttcggcg aggattatga gcaggacaac tgcgagtgtt
    13021 gtgacaactg cctgcatccc aaaaagcaat ttgaagggca ggaagaaatg tgccttgtgc
    13081 ttgaaacggt tcagaaatcg ctgcaacagt tcaatgccga acatgttgcc aatatcatag
    13141 ccggtgtatc caatgccatg atcaaatcat accagcacca caagctggat gtatatggaa
    13201 tgggtaagga caaaacccca aacttttgga atgccatcat ccggcaggcc atagtactcc
    13261 gttttctgga caaggacatt gaaaagtacg gagccctgtc ggtcacacct gccggcaggg
    13321 atttcctgga aaatccaacc accctgatgc tctatgaaga ccggcagatg gaagaagaag
    13381 aggattcaga aattgaagct gctgccaagg ccggaggagg cggaggggat atcgagttgc
    13441 tggccatgct gaaagacctg cggaaagata tggcacacag gctgaaactg gctccttttg
    13501 tcttgttcca ggatgtgagc ctggaagata tgtccatcag ctatcccata accctggaag
    13561 aactggcccg ttgtcagggt gtgggtgagg gaaaagcaag aaaatacggg aaacctttta
    13621 ttgagctgat tgaaaaatac gtcgaagaga acgaaataga ccggccgcag gatattgttg
    13681 tcaaatctgt tataaacaag tcccagaaca aggtgtatat catacaaagc attgaccgca
    13741 agctggattt tgtagatatt gcagaagcca aaggccttac tatcgatgaa ttgctggaag
    13801 aaatagaagt cattatcaat tcggggactc acctggatat agactattac ctgaggcaaa
    13861 acatagatga tgataagctg gagaccatct atgactactt ccgttacgat gcgaaatccg
    13921 aatccttgtc agaggctgtg aaagaactgg gtcctgaata tacagaagaa gaagtgaggc
    13981 tggtccggat aaaattttta gttgaggttg ccaactgaag ttttatcttt gtcttttatg
    14041 cgaaaacgta ccatcatatt ccttgtgctg ttgctgctgt tgctggcagg ggcttcggtg
    14101 tatggttttt ttctcctgtt cagggattcc ggtgatcatg ctgcaggcaa cagcacctac
    14161 agggctgttc ctgtagatgc cgttctggtc cagcatttca gcggattgaa caccctgact
    14221 ggagaggtcc ttactcccgg aagttacctg gaatccctgt ttcaccccga acaggaaact
    14281 caggcattcc tggcggaact gacggctttc cttacggccg cttatcccgg gatggcacaa
    14341 gccgagacac tctgttcgct gcacccctcg gcaaaaaata ccctggcggc ccttttctgc
    14401 atatcaggtg aaaaagcgca ggatccgcaa tggtgggata attttctggg aaagagcctc
    14461 ttatccttca aaacccggtc atataacgga caaaccatat accaggcagg aggagaagag
    14521 tcggaccata gtgtttatgc ttcctgtgtc aatgacctgt tgatggtatc ttcttctctg
    14581 gtagtgctgg aatcatccct gcgccacctg ggcaacggca gctctcttgc ggacgatcct
    14641 tcctttgccc ggttagtgga acaaaccccg gtttccaaac caacccgtat ttttattccg
    14701 caccagggga ttccttcaat gtttgccgct tatctgggga tgcccttgca aaagtacgct
    14761 gcttttgtgc gctctgctgc ccgctggacc gtgctggacg gattcataag cgggcatgaa
    14821 gtgaagatgg acggatattc actgtttccg tccggggcgg agcattattt tgccaccttt
    14881 ctgggccagg aaccccagaa gctcatggct ccgcaaatcc ttcccgcagc ggctgtggca
    14941 ggtatttcca taggcatatc ggacctcgct tcttacctgg aaggatatgg aagatatctt
    15001 gagttgcaga aatccaatag aaaagggcct gataaagaaa ggctggaatg gttcagtctt
    15061 ctgtatccaa cggaagtatc actctcctgt gttccttttg ggggtgaatt gcaatggata
    15121 gccgtgatac attccaggta tatccaccag gcccggatac aatttgccct gctcaacaga
    15181 caggaggaaa acaaggtgat ggagaatccc gttcccggtc ttctgcctga aatcttcggt
    15241 ccggttttta ctttatgcca ggcatcggat tactgctata taggtccgtt catacttttc
    15301 ggaccccgga aactgctgaa cgggattttg aaaagcaatc tggatggtaa ttaccattca
    15361 ctggctgctg ccatttccca gaccgatgcc tcaccaggtg ttatgaatgc ctccaacctg
    15421 acgttattcc tccagcccac ggcaggtctg aaacaaatcc agaccttgat ggataaacgg
    15481 tatgcagagc gactggatgc atggggccga tacaatgcgc agtatacatt cctgcagttt
    15541 tcatcactgg aggaccgtct ctatgctcat gtctctgtat tcggggattc actggagaca
    15601 tctcccctga taccacgtca gcccgaagca cgaattcgca gtggtatgca ccaggatacc
    15661 ctggccaggg gaagaccgcc cttcaaggtg tttaaccatt tcaccaaaaa ggacaatgag
    15721 ttgttccaga ctccctggcc cgagtgccgt cttatcatga gagaccacac gggtaagaca
    15781 ttatgggaaa agaccatgga agggcccatc caggacgggg taagacagtt ggacctgctg
    15841 aaaaacgaca aattgcagat gttgttttcc ataggaaaca ggttgtattt gttggacagg
    15901 ctggggcgca atgtatcccc ttatcccaaa gcttatgctt caagcatcct gtatggtcct
    15961 tttgtttttg atccggaagg aaagaaagaa tacaggatcc tgctggcaca ccaggacaat
    16021 gtgctgcgct gttatgacaa attcgggaat gtacccgaag ggtggagcga ttttactttg
    16081 cccggatacc tcaccggaca accccgtcat accaggtggg aaaacaccgg cttatggata
    16141 gtctacacag aagcagggac tgtcatttta tctgaaagcg ggcaggttag ggggatgact
    16201 acccggaaag aatgcctgga tccgcaggcc gaagtcagga tttcaggtcc gtatgaactt
    16261 tacggcacaa caattgaggg aaagaccctt gccataatgc tggaataatt aggatgagcc
    16321 ttctgaccat aatcttcttt atcatagttg ccggatactt tttcataaat cttatcccac
    16381 tgatgttgtc aaggaagttc cgtaaaatgc aggaacaggg ccgtaagcgg gacaacattg
    16441 aaaacaacac ctcccggaaa aagcgtatag acaagggtgc cggagaatac gtcgattatg
    16501 aggaaataga cgaatgatga aatcattcat gcggatactg gcttatggaa aaccatacag
    16561 caggtactgg ccttcttacc tggtactttc tgttttcagc gttttctttg gcatagccaa
    16621 ttatgccctg gtggctccct tgctgaccgt cctgtttcag gccgataccc tgccgggaga
    16681 aatggcccgg cccgtattca gcttatctat cccttatttt gttgacctgt tccgttatta
    16741 tatggtcatg ataaaagcga aggacgggat actgatggct ttggtgtatg tgtctgtcag
    16801 cttgctggtt gcttcctttc tggccaacct tacatattat ctttcccaga ggatcctggt
    16861 ggatatgcgt gccaacctga tgaaaaacat caggaacgat cttttttcca agattagccg
    16921 tctgcatata gggtattttc atgaccagcg taaaggggac cttctttcca ccgtttctaa
    16981 cgatgtgaat gaagtgcaga actccgtagc cggctctttc catatcattt tccgtgatcc
    17041 tttgctcatt gcgggattct tgtccatgct cttttacatg agtccgaagc tgaccctggc
    17101 ctccttgctt acactgccgt tgtcggcact gctcataggc aggatcaccc ggtcactgcg
    17161 ctcgggtgcg gaagtatccc agagtcttct gggtaagttg ctgagtcatt ttgaagaggc
    17221 catatccgga agccggatcg taaaggcttt caatgcacag gggtatatcc tggatcgctt
    17281 tgacggtgtt aacgaccagc accggatggc aaccaggagg ttgtacaacc ggcaggaact
    17341 ggcttctccc ctgggcgagt tccttgggat ttcagtggca gctgccgtgc ttttttacgg
    17401 cggctggctt caggtcaggg gggacctggg aatgacatgg cccgaatttg tcgtatacat
    17461 tggtttttac tggcgtgttc ttgagcccgc caagtccatt acacgaactt atgccgtgat
    17521 ccgcaagggc atggtatcgg cccttagaat cttcagcatc ctggatgcac cggttgaaat
    17581 aagaaaagac caggatgcca tgcctgtaaa aaccttcagt gaacgcatag aatatcgtaa
    17641 tgtcagtttt cgctacaagg aaacagatcc ctatgtgctc agggatataa accttgtgat
    17701 acctaaaggg aaggttattg cccttgtggg accttccgga gccggcaaaa caacactggc
    17761 agacatgctg ccacgtttct atgacgttac ggaagggtct gttttgcttg atggtattga
    17821 tatccgcaaa tacaggccca gggatcttat tggacttatg ggcatagtaa cccaggaatc
    17881 catcctcttc aacgatactg ttgcaaataa tattaccttt ggccttgtaa atatttcgcg
    17941 tccggaggtg gaacatgcag cccgggtagc caatgcacat gatttcatta tggaattgga
    18001 gcacggatac gaaaccaata tcggggacag gggtgaaaaa ctgtcgggag gacagcggca
    18061 acggttggcc attgcaagag ctgtgttcaa gaacccgccc atacttatcc tggatgaggc
    18121 gacatcggcc ctggactcgg aaagtgaaag gatggtacag gaagccctga ccaaactaat
    18181 gaaaaacagg acttcggtgg tgattgccca ccgcctgtcc actgtcaggg atgcagatga
    18241 aattgtggtc attgacaaag gacgcatcac agaacgcggc actcacagcc ggttgctgga
    18301 acaaaacgga atgtataggc acctctacga tttacaagct tttggataat tctaaatact
    18361 agacaaaatg agtaaggata ttttaaagac tgaatccctg aatgagcttt ttgaaaacag
    18421 tttcaaaaag tactgggact acaaggccct gaccgaattt aacgggcaga ccctatatta
    18481 tagtgacctg gcacgacgta tccggatgat tcacataggt tttgaaaaat gcgggctgaa
    18541 gaagggagat aaagtggctt tatgcgggaa aaaccagtcc aactgggctg tggcttttct
    18601 cgcgactatt agctacggag cagtggttgt acccatattg catgaattca aaccgggaaa
    18661 catccagcac ctggtaaatc attccgatgc acgcatcttt tttgtagggg aacagatatg
    18721 ggaaggactt aatgaagctg agatgcccaa tctggaagcc attgtggaat tgaatgattt
    18781 cagcattctt tatactcccg acaaagaaat catcaatatc cggaaaggat tggagaaaaa
    18841 tttcacagag ctttatcctg aagggatgaa accggaagat gtccgtttcc accgggacca
    18901 gcccgaagaa ctggccctga taaattatac aagcggtaca acaggtttct ccaagggtgt
    18961 catgctgcct taccgttccc tgctttccaa tgtactattc gcatgggagg tgctccccca
    19021 ggtgagtaac ggggccaggg tcgtttccat gcttccctcg gcacacatgt atggactgat
    19081 gtttgaattg ctctttgaaa tgacatccgg ctcacacgta catttcctga ccaggatgcc
    19141 ttcacccaag gtgatcgtgg aagccttcca gagtgtcaaa ccccgcgtga tcattgcggt
    19201 tcccatgatc attgaaaaga tatacaaaca gaagctcctg cccctgctca agcgtccttc
    19261 cataaaggtc atgctgcgca tgcctttcat tgaccagaga atcaaaaacc gtattcacga
    19321 cgagctggtc aaggcattcg ggggggaatt cgtggaaatc atcgtgggcg gtgcgcctct
    19381 gaaccatgag gtggagccgt tcctcaagag cataagcctg gcctttaccg tagggtacgg
    19441 gcttaccgaa tgtggtccca ttgtatctta tgccccctgg aatgaaagac cgctgttttc
    19501 ctgcggcagg gcagccccca ggatgtccat aaggatagat tcggaggatc cggagacggt
    19561 tccaggtgaa atccagctta agggggacaa cgttatggta ggatattaca agaatccgga
    19621 agccaccgag caggtgttta cagaggatgg ctggttcagg acaggagaca tgggaaccat
    19681 ggacaaggac ggatacctgt atatcaaagg acgctccaag accattatcc tgggacccaa
    19741 cggacagaat atatatcccg aagagattga aagttccatc aataacatgt catatgtaaa
    19801 cgaaagcctg gtgatcatgg aagataacaa gctagtggct ctggtttatc ccgattttga
    19861 aaaagcggca gcagaggggc ttcccgattc tgcgatggat gcgaccatgg aggccatgcg
    19921 tgcgcagatc aacctggatt tacccaaata ctgtcagata gccagggtaa ggatagtccc
    19981 ggaagaattt gaaaaaacac ccaaaaaaag tattaaacgt tttctataca atggataagc
    20041 agacccagtt tgacctccat tatctggaaa tggcccggat atggtctcag aattcttatt
    20101 gtgaaaggag gaaagtcggg gcattgatgg taaaagaccg gatgatcatc tcggatggtt
    20161 ataacgggac tccccgggga tttgagaatg tatgcgaaga tgaggagggg cataccaaat
    20221 cgtatgtttt gcatgcagaa gcaaatgcca taactaaagt ggcaaggagt aacaacagca
    20281 gcgaaggggc caccatttac attacggctt ctccctgtat tgaatgcgcc aagctgatca
    20341 tacaatccgg gatagtccgc gtggtgtaca gcgatgatta cagattgcag gaaggaattg
    20401 aacttttaaa aagggcaggt attcaggtcg acaaatttta acagataatg gaagggttta
    20461 gaaggctgcg gaaaacgctg ggcatagtgc tggtcatact cgttgcactg gccataacag
    20521 gtatcataag atgggatatt gtcaggaaac aaaatggtgg gggaaggaaa caacttgggc
    20581 gggagtggtc caagctggag ctgatcctgg aacagatcca gaataattat gtcgatccgg
    20641 tagacgtttc ggcgtttata gagaaaacgc ttcctactat catggaagag ctagacccac
    20701 actccgttta cctttcaccg gatgaattgc gaactgccga tgaagaattg cgtggaaaca
    20761 tagaaggaat aggcattgtt ttcaatgtgc ccagcgatac ggctaccgtg ataaatgtcg
    20821 tggtgggtgg accatcagag aaaatgggcc tgcaaccagg ggacaagatc ataaaaatcg
    20881 acacccagat cgttgccggt gtccagatgg accaggattc catagtacgg cttctcaagg
    20941 gacccaaggg cacccaggtg gatgtgcacg tgaagcgtta tggagaaaat gacctcatac
    21001 acttttccat cgtaagggac aagattccgg tcaacagtgt ggatgtggcc tatatggcag
    21061 attcactgac cgggtatatc aaactgtcaa ggttttccat gacaagctat gccgaagtcc
    21121 ttggcgccat tgcagatttg atgaaacagg gaatgaaaca ggttatcttt gatcttcggg
    21181 acaattcggg aggctacctg gaccaggccc tgaaaatcac aggtgaattc ctggatgaga
    21241 acaaactggt tgtttatacg gaaggagagc accgggaaag acaagatttt tacacaaaaa
    21301 aacggggatt ctgctatgat atgccactgg ctgttttgat caacgaagca tcggcctcat
    21361 cttctgaaat catggcggga gctttacagg ataatgacag gggtaccatc atagggcgcc
    21421 ggtcatacgg aaagggcctt gtccaggaac ccatttattt ctctgataat tcaggattgc
    21481 ggcttaccgt tgcccgcttt tacacgcccc ttggcaggtc cattcaaaag ccgtacatga
    21541 gcaactcccg cgaatacgca tacgaccttc tggaacgtta caagcatgga gagctgatgc
    21601 aggaagattc catccccaaa aatgattcgc tgcgttttgt aacacaggcc ggaaaagtcc
    21661 tgtacggcgg tggcgggatc attcccgatg tttttgttcc cctggacaca acaggggtaa
    21721 acgacctcta cctgcgcata tccaggcaga acctgcccat ccggttcagc atggactttg
    21781 ccgatcgtaa ccgtgtacag ctatccggca tagacactta tgacgatttg gagcaatttt
    21841 ttgcatcaac acagctggaa gaagaattcc tgcgctatct gaacaaacag ggaatccatc
    21901 cgctgcctgt gcaatggaag gagtgcggag gcatcctgct gacgcagatc caggcatata
    21961 ttggaaggaa tacccggctg gaagacaaag gtttctatcc ttacctcggc aggatagata
    22021 atgtgttgat caaagcgctg gaagtgctgg gaacaccgtt aataaccgaa ccggcggagc
    22081 ctggtcttgt tttcgcgcca atggggaaca accttgacga ataattccag aaatactttt
    22141 ttctggaaaa aatcctccat atccagacgg gcttgtgttc ccacgcgttt aagcgcactg
    22201 cctttgtcac ctattatgat ccctttctgc gaatcgcgca tgacactgat cactgctcgt
    22261 atgtcatacc ttgcctgact ctccagaaat tcttccacgg tgacctccat gcaataggga
    22321 atttcttttg aataattcag gaagatcttt tcccggatta tttccgaggc gaaaaaacgc
    22381 aggcttttgt ctgtcagctg atctttgtca aaccagggag gtgaggccgg cagcaaagcc
    22441 aggatcctct ccagtagtga atccagattg aagtcttcca gggcacttac cgggaaaatt
    22501 tcggctttgg gaaccatggt gtgccactga tccatcagtg acaagacttc ggcctgagaa
    22561 cttttatcaa ttttattgag aaccaccagg acaggcacct caatccggtt caacttttca
    22621 atataatcag gatttttagc cagcttttcc acaacatcgg tgacgtatag caggatatcg
    22681 gcatctgcaa gggcggattc tacatattcc atcatgttct cctgcagcag gtaatagggt
    22741 ttgatgatac ccggcgtatc ggaatacacg atctggtaat ccggcccgtt tactatgccc
    22801 atgatgcggt gacgggtggt ctgtgcctta gaggtgatta tggataattt ttcacctacc
    22861 agggcattca tgagcgtaga tttgcccaca ttgggattcc ctattatatt gacaaaacct
    22921 gccttatgtg tagcactcat gttattggta gaatcccgta atcaacatat tgacctcttc
    22981 ttccgtcaaa aagcgccatt gtccgcgttt gaggtttttc ttggtaagtc ctgcaaaata
    23041 gacgcggtca agttttttca ccttatagcc aagggcttcg aaaagacggc gcacaacacg
    23101 gtttttcccg gaatgaattt ccagtccgat ttctttctcg tcattgtcta tgtaagctat
    23161 ctgatccaca tgcatcatgc cgtcctccag ctctattcca ttgacaagct tttccatatc
    23221 ggattgtttc aggttcatgt ccaggcgtgt ctggtaaatc tttttccggt tgtaggacgg
    23281 atgtgtcagc ttttctgtga gttcgccatc attggtgaac aagaggacac ccatggtcat
    23341 tttatcgaga cgtcccacgg gaaatacacg ttgttcgcat tttcccgcaa taaggtccat
    23401 aacggatttt tcggcatggt catcgcttaa ggttgtgacg taccccctgg gtttgttcat
    23461 cacaatgtag gttttgggtt cgcctttgat ccgctggccg ttcacgcgta catcgtcggc
    23521 cggtgttacc tttactccca gagtgtccac cacttttccg ttgactgtga ccagtcctgt
    23581 tgtaataaag gtatctgcct ccctgcggga acagagaccc gagttggcta tgtatttgtt
    23641 gagccggact tcacccggca ctttaggttg ctttggttgg ggggcttctg tttttttagg
    23701 agccggttta tgaccgaagg atttgtatgt cctttcagag cggtaggatt tttcgcggcg
    23761 cggtctttct gagctgtaac cgccttcgcg gcgtggtctt tcagag
//
LOCUS       DAOSOF010000009        23104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2241_length_23104_cov_4.662068, whole genome shotgun sequence.
ACCESSION   DAOSOF010000009 DAOSOF010000000
VERSION     DAOSOF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_23104_cov_4.662068"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLM68_01070"
     CDS             <1..498
                     /locus_tag="PLM68_01070"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOT53996.1"
                     /translation="MEWFTKLNVGNLWPLILVAVGILIFTKARKGDHHYSRSGNESWH
                     DRPSSGSTRFNNGYVDYSMVFSGSDTVFLEPVFRGGNIESVFGGVTLDLRNTALQEGV
                     SYLKVSSVFGGVTLFIPPSWNIEIQSDSVFGNFKDNRPYTAGVDKNSKLIIKAECVFG
                     GGEIR"
     gene            509..1240
                     /locus_tag="PLM68_01075"
     CDS             509..1240
                     /locus_tag="PLM68_01075"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT53997.1"
                     /translation="MATGSVVLAIALLFAWLTGSLISLAWFEMLADGFIIAACMTGLG
                     LYTNKMDTYGMPPYVQSLIFCVGIAGIPVLAMYLYLDPDGFRNFVRIIPLRIFIAALI
                     CLVLLFLKKAGKAGKTDSPLPEKSLSASSPSQGYQDNIMVRSGNGIKVIPVNDLLYIQ
                     ADGDYIWLVAKDGKWMKEETMKHMQETLSPTRFVRIHRSFIVNVGAISRIERYGQQQL
                     IILTNGDSIRISTSGYRTLRDALGF"
     gene            1280..2674
                     /gene="ahcY"
                     /locus_tag="PLM68_01080"
     CDS             1280..2674
                     /gene="ahcY"
                     /locus_tag="PLM68_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT53998.1"
                     /translation="MDYKIRDLELAGFGNKEILLAEKEMPGLMALRRRYADKIPLRGA
                     RITGSLHMTVQTAVLIETLRMLGADVRWASCNIFSTQDHAAAAMAASGVPVFAWKGET
                     LEEYWWCTLQALSFPDGKGPHMVVDDGGDATLLIHKGVLAEKDPSSLDKLTGNEEEKI
                     IAALLRKTLQENPGIWTRMVREMKGVSEETTTGVHRLYQMFEKGDLLFPAINVNDSVT
                     KSKFDNLYGCHESLADGIKRATDVMIAGKVVVVCGYGDVGKGCARSMRSYGARVIVTE
                     IDPICALQASIEGFEVRKLENCLDEARIFVTATGNCDVIRIDHMEKMRDHSIVCNIGH
                     FDNEIQVDRLTNYPGIRKVNIKPQVDQFFFPDGHSVILLAEGRLVNLGCATGHPSFVM
                     SNSFTNQVLAQINLWTIQHELGVYLLPKHLDEEVARLHLDYLGAELTPLTKKQADYIG
                     VPVNGPFKPDYYRY"
     gene            2680..4071
                     /gene="asnS"
                     /locus_tag="PLM68_01085"
     CDS             2680..4071
                     /gene="asnS"
                     /locus_tag="PLM68_01085"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT53999.1"
                     /translation="MTKSRIKIVDLLKMQAGIPVMVKGWVRTKRGNNKITFIALNDGS
                     TIHNVQIVCDMNVFTEDMLRPVTTGACLRVSGQLVASPGKEQAVEIQAEEIEIYGTAD
                     ATVYPLQKKGHSMEFLRDIAHLRPRTNTFGCVLRIRHAMAFAIHKYFNDKGFVYLHTP
                     IITASDCEGAGAMFQVTTLDLNNLPKDTDGYVDYAQDFFGRQTSLTVSGQLEGELGAM
                     ALGEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIHDNMDLAEDFIKYLVRYALE
                     HCASDLQFLNNIIDKSLIERLEGVYNIEFVRLTYTEGIEILTNSGQQFEYPVYWGVDL
                     QSEHERYLVEKHFKRPVILTDYPRDIKAFYMKENPDGKTVRAMDILFPKIGEIIGGSQ
                     REENLEVLVRKMKEMHVSQENLDWYLDTRRFGTGPHSGFGLGFERLLLFITGMTNIRD
                     VIPFPRTPKSADF"
     gene            4074..4688
                     /gene="udk"
                     /locus_tag="PLM68_01090"
     CDS             4074..4688
                     /gene="udk"
                     /locus_tag="PLM68_01090"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOT54000.1"
                     /translation="MLVIGIAGGTGSGKTTVVNKILERLDERQITVIPQDSYYKDNSH
                     LPLEKRQELNFDHPQSIDWELMVDHLLKLKSGYAIEQPIYSYITCTRSAETIYTKATQ
                     IIIVEGILIFTDPPLRNCLDMKIFVDADADDRLSRVISRDIIERGRSVNKVLERYEKT
                     VKPMHLQFIEPTKRYADIIIPQGGHNTVAINMLISTIEKALLPS"
     gene            4701..5411
                     /locus_tag="PLM68_01095"
     CDS             4701..5411
                     /locus_tag="PLM68_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54001.1"
                     /translation="MKQESRIGLYLTIIIHLVALIVVLSLQVSSFIQKESTYILDFTP
                     QEELEMEQQRERIRAEVSEGLDDLIAGIQKESRNVAVDATLRNRPLRDDRHADPSQVY
                     KEAEELQKRLDAARQEIEEQGGMEEISMTEKEENDQETIPYTGPAVLEYTLEGRKAMS
                     LPIPVYKCLGGGDVTVSIQVDRKGYVQKAAIVAYFSSQDRCLQEYALRAARQSRFTAS
                     VDAPPLQTGEIVYRFIPQ"
     gene            5436..5882
                     /locus_tag="PLM68_01100"
     CDS             5436..5882
                     /locus_tag="PLM68_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54002.1"
                     /translation="MDEREYREIKELLAQLTARIDALYTPAPAPVPVPDTPGYFELET
                     PSVPVSPTLKDKFSGLDRRPTLADSLAKTKNKGLLKEVIPLNDKYLYLKTLFSEDSGL
                     YEKTIADLQSFKDMQQAEAYLAQHFPSWELSTPAVQKFLTQLENVF"
     gene            5872..6567
                     /gene="rsmI"
                     /locus_tag="PLM68_01105"
     CDS             5872..6567
                     /gene="rsmI"
                     /locus_tag="PLM68_01105"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT54003.1"
                     /translation="MCSKLIIVPTPIGNLEDITLRALKVLQEADLILAEDTRTSSVLL
                     KKYAIKTHMVSFHQYNEHKQVAPVLEKIRQGKLVALISDAGTPGISDPGFLLARECLE
                     EGIPVECLPGPTALIPALINSGFPADRFCFEGFLPVRKGRRSRMEVLAAETRTVILYE
                     SPFRLVKTLQQLEQYLGRDRMACVCRELTKIHQETRRGTLAELSQWYGQNTPKGEIVI
                     VLKGRGKDHETEQ"
     gene            6551..7333
                     /locus_tag="PLM68_01110"
     CDS             6551..7333
                     /locus_tag="PLM68_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT54004.1"
                     /translation="MKRSNRHHYIIHATGIAGIIIILVIKIFWEHLLPGHQTGRDSRM
                     SEEMLNLYAEPPATASGKSTVPSGQWTSATASREKPITGRQENPVPAPGERPATGRQS
                     ENHPDRGMESAAEPGKSSPGKYNPATHMKIELNTADSAELVKLYGIGPYYAVSIMKYR
                     EKLGGFAIPEQLLEVKGMDSERLEGFYDRVFADTSFISKMDLKTATEDQLAQHLYIGR
                     YLARCIVRYRETAGVDSCTLEHLVRDKVLTGEQAVKIGWYLY"
     gene            7392..8321
                     /locus_tag="PLM68_01115"
     CDS             7392..8321
                     /locus_tag="PLM68_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54005.1"
                     /translation="MNILETKNVVKSFDGLLALDAVSIAVPRGLVFGLLGPNGAGKTT
                     LLRIVNQITAPDSGSIFFDGHPLAAEDVFSIGYLPEERGLYKKMKIGDQALYLAKLKG
                     MTHSEALASLKSWFIRFGIQAWWNKKITELSKGMAQKVQFVTTVMHNPKLLILDEPFS
                     GFDPINVDLIRNEILRLKENGTSVILSTHNMESVEEMCDQIALIDNARLMVTGNLHEI
                     RRIHGDNHIEILYRASEPINPLNLGVVPFNIVSQGKKDGSYQMVVDPADKNDTNAVLR
                     SLMPHVSVVGMTEIIPRMHDIFIKLVTNKEERK"
     gene            8318..9661
                     /locus_tag="PLM68_01120"
     CDS             8318..9661
                     /locus_tag="PLM68_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT54006.1"
                     /translation="MNIKKINLIISREFAIRVRKKSFIIATFLTPLMMAALITVPMIM
                     SGVRDDREKVIEVIDPSGIGQAVLENQEYITFNFRQEGDLEAYRAGFEDKNLHAVCVL
                     EDTGNNSVDIRLYSTGQIDSDVQRYISRQVSQEVERRKLNTYDIPQLQEIMQDVRTNI
                     QAKTFTWTDTGDEKETIKEVYMAIAYLSSFLIYIFIFMYGSMVIRGVIEEKTNRIVEV
                     IISSVKPVELMLGKIVGIALVALLQFLLWILLTVIILLIVMAMVGTGTLGEMGQAAPQ
                     IQGGLAGTIAAFDTGNGVLTGIASTLSQINAGKFLLSFMLYFIGGYLMYASMFAAIGS
                     VSDAETDTQQFQLPITIPLILGLFIMMHTFQHPDSSLSFWASMIPFTSPMVMLARLPF
                     GGVPLWQLLLSITLLFGTFLGVVHFSAKIYKVGILMYGKKVTWKELWKWMRYKNG"
     gene            9637..10413
                     /locus_tag="PLM68_01125"
     CDS             9637..10413
                     /locus_tag="PLM68_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT54007.1"
                     /translation="MDALQKRLIRIVFLSALLLIARMTTSLAQTINATRVLMDTSAYQ
                     AKASKAVKQIIDTYSPELNRKMGEVVGYTTMAMDSYPPESPLSNMAADALLAIAKRHY
                     GLDKVDFALTNFGGIRTSFPKGDLSLYYVYAAFPFENSLVLVALKGTQVKALFEIFAR
                     SHAEALAGVRFRADNRKITKLLINGLPLDEDRVYYLATIDFLLSGGDRMTALRENLGV
                     DYSGITLRDVILEYIGVFTKNGEPIPARKDGRVVINNPRE"
     gene            10413..11222
                     /locus_tag="PLM68_01130"
     CDS             10413..11222
                     /locus_tag="PLM68_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOT54008.1"
                     /translation="MKRFFTIVLSLVLLAAISRPLSGQDLVIVHTNDFHSQIEPFQSG
                     RNAGTAGLLSLSGYLEESRMVCPDLLYLDAGDYNQGTPYFNLFGGQVEVEGMNALGLF
                     ATTLGNHEFDNGMEDLVRRLKKADYHVLCANYDIQHKPLRRLVKPYVITRVNGKKIGI
                     IGLTLDLNGLVSSEVLRKMNYRDVILTANRMASLLKRKGCDLIICLTHQGLDSDMEMA
                     AQSRNINLIIGGHSHTFLEEPVKKADLDGQEVVIVQAGAYGVYAGRIDITF"
     gene            11235..11798
                     /locus_tag="PLM68_01135"
     CDS             11235..11798
                     /locus_tag="PLM68_01135"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOT54009.1"
                     /translation="MFLETQKWNGRIEVICGSMFSGKTEELLRRLKRARFANQRVEIF
                     KPRIDVRYSTDDVVSHDSHSYPSTPVDSAESILLLATNVDVVAIDEAQFFDQNLVRVC
                     QILANNGIRVIVAGLDMDYQGNPFGPMPQLMAVAEHIVKVHAICVRCGDPAQYSHRLS
                     DSTRLVELGEKDIYEPLCRHCFNQIKK"
     gene            11795..12901
                     /gene="alr"
                     /locus_tag="PLM68_01140"
     CDS             11795..12901
                     /gene="alr"
                     /locus_tag="PLM68_01140"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT54010.1"
                     /translation="MNDNQFTHLEINIPNIRHNLNYFRSFLNKKTKLLVLVKAYGYGH
                     GIGEFSRILEENGVDYLAVAFPGEGVAIRDAGVKLPVIVLSAGMEHYSLLIKNQLEPS
                     LPCLEAFRRFSEEAAVMGEKGYPAHIKLDTGMHRLGFEEEELRFLADELPLTQSVRIA
                     SIFSHFVASGDPRHDDFTRTQIARFESLSSFLMQHLDYRPIRHLLNSPGIERFAKEAE
                     YDMVRLGIGIYGMSYVDRSRLRPTAYLKTPVLQVKTVAQGETVGYARAGVVTAPGTHI
                     ATLAIGYADGVNRHLSGGKACFSVNGHRAPTIGNICMDMCMIDVTGIDVNPGDMVTVF
                     GDDPTIFELAEILGTIPYEILTSVAHRVPRIYVE"
     gene            complement(12902..13945)
                     /gene="holA"
                     /locus_tag="PLM68_01145"
     CDS             complement(12902..13945)
                     /gene="holA"
                     /locus_tag="PLM68_01145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT54011.1"
                     /translation="MAKTTSKETITTFKRIMKDLEEGRYKPCYLLMGEEPYYVDKITS
                     FIAENALTEDEKAFNQMVIYGQDATVMQIADNARRYPVMASRQVIIVKEAQQLKGPDG
                     LVSYLKAMNPSCVLVLCFMGKSADKRTEFWKTAQKYCEILESVPFRDYQMQEWITTYL
                     KEKKTSIEPQAAALLADFLGTDLQRVVMELDKLFILIPEGTWAITTDMVENSVGINRD
                     FSPFTMFKHLTDGNFAGVQPIVQYFSENDKKYPLVMIISLLYVHLSRILRYHALRMEN
                     PSLSQEGIGKLMGGNYYFFKEIIQASRFFTFRRTVRAMELLCFYDSKYKSSDRGEASD
                     GELLMEMVCKMMA"
     gene            13972..14388
                     /locus_tag="PLM68_01150"
     CDS             13972..14388
                     /locus_tag="PLM68_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOT54012.1"
                     /translation="MNSPSAIYCAVRKKWVERTPEEEVRQHIIKHLNIVCQAPLHLMT
                     CEYPVQHLNQRADLVLHDREGKPLVLAEFKAPAVRVDDKTIEQLRRYNEEIMAPYLLI
                     SNGSQTFFWKLDRVNGSYQSMKDIPPYQDLIIKNPH"
     gene            14388..15791
                     /locus_tag="PLM68_01155"
     CDS             14388..15791
                     /locus_tag="PLM68_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT54013.1"
                     /translation="MKDPLKPGIFKIVSRQAAARNVRAYVIGGYVRDHFLKRPCTDID
                     IVVEGNGMELASDVASVLKVNATLFKNFGTAMLRYRGLEIEFVGARKESYSTDSRKPV
                     VENGTVEDDQKRRDFTINAMAFSLQQEDYGSLTDPFNGLNDLHDKVIRTPLDPDKTFS
                     DDPLRMIRAIRFAAQLEFTIDNTAFNSIQRNADRIQIVSQERITTELQKIMASRKPSA
                     GFYLLEKSGLLAIILPQVALLQGVETMEGKGHKENFSHTMEVLDNVASMTDNIWLRWA
                     ALLHDIGKPSTRHFDKETGWTFHGHDFVGSKMIPGIFRQLRLPLGEEMKYVRKLVQLH
                     LRPIALVNNVSDSGARRLLFEAGEDIDDLMLLCRADITSKNPMTVRHHLGNFEQVSKK
                     LIQVEEKDAIRNFQPPISGEIIMKTYNLPPCREVGLIKDHIKEAILDGKIGNNYEEAL
                     DLMKQRAAELGISEAGA"
     gene            complement(15772..16359)
                     /gene="folE"
                     /locus_tag="PLM68_01160"
     CDS             complement(15772..16359)
                     /gene="folE"
                     /locus_tag="PLM68_01160"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOT54014.1"
                     /translation="MSYTNNDHFTPETTRKLEELYKEALQLLGEDTEREGLLRTPLRV
                     AQAMQFLTKGYLEDPAAILNSAKFREEYQQMVLIRDIEMYSLCEHHMLPFYGKAHVAY
                     IPNGFITGISKITRVVETFARRFQVQERLTVQIRDCIQETLNPMGVAVVIEAEHMCMQ
                     IRGVQKQHSKMVTSAFTGTFLKDMRTREELMHLLH"
     gene            16497..17210
                     /locus_tag="PLM68_01165"
     CDS             16497..17210
                     /locus_tag="PLM68_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT54015.1"
                     /translation="MEKEVLIKIRGIKKIYKMGTQEVRALNGIDLEIRKNEYVAIMGP
                     SGSGKSTLMNLLGCLDTPTEGTYILNGTDVSQMRDDQLAEVRNKEIGFVFQSFNLLPR
                     YNALNNVALPLIYAGLPRQEREREAKKALVNVDLEDRMDHKPNELSGGQKQRVALARA
                     MVNNPSIILADEPTGNLDTKTSLDIMALFEQVYAKGNTIIVVTHEEDIARYARRVVRL
                     RDGLVESDEINANPALGNS"
     gene            17207..17758
                     /locus_tag="PLM68_01170"
     CDS             17207..17758
                     /locus_tag="PLM68_01170"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT54016.1"
                     /translation="MSKIYTGNGDNGSTSLQDGSRVSKAHPRVAAYGKADELISWIGL
                     IRSHPDFSSDGELHEINHFLRKIQVGLMYLARRLASNDSSRLFYYDFETATKALEESI
                     DIMQARLPAIHSFILPYKPVISSHIHIARTICRETERYMVVLGTDIASADMLTYINRL
                     SDYLFVLARFMTVTNKCEEDNFV"
     gene            17788..18009
                     /locus_tag="PLM68_01175"
     CDS             17788..18009
                     /locus_tag="PLM68_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOT54017.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYATRSGAPLEVIENLSDLE
                     DDGEIYDSIEDIWPDYPSKEDFFFNEDEY"
     gene            18019..19371
                     /gene="trkA"
                     /locus_tag="PLM68_01180"
     CDS             18019..19371
                     /gene="trkA"
                     /locus_tag="PLM68_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOT54018.1"
                     /translation="MNIIIAGAGEVGFHLAKMLSDENHQITIVDKNEARLNQIAETSD
                     VVPVAGIVTSISTLEKAGISRCDLFIAVSPAEEQDMNIVAALLAKQLGAKRVTARINN
                     NEYLKQENRVLFTEMGIDLLFYPENIAAHEIVDLLKQSGTSEFMDFSGGRLQLIVLKL
                     QESSSIVNKSLKQVAEENGGNIPFRAVAIAREGNTIIPRKDTVFQLHDLVFVITKREA
                     AHAAMVLTGKENMVVRNLMIMGGGRVGEMVAHQMESAIEIIRIVEIDSNRCRELTVSL
                     PRSLIINGDARNTDLLLEEDLTQMDAFVAVTSSAEANILSCMAAKRAGVKKAIAQVEN
                     LDYIKLAESVGVDATINKKLITASRIFRFTMTGDVPTMKCLNGSDAEVMEFIAKPRSL
                     VTQKIVRDLGFPRDAIIGGITRGNTSLIVRGDTEIKPYDRVVVFSLPSALPTIHRFFV
                     "
     gene            complement(19375..19824)
                     /locus_tag="PLM68_01185"
     CDS             complement(19375..19824)
                     /locus_tag="PLM68_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT54019.1"
                     /translation="MKNKNNLIKRVTRCTRGWLLTNPVLFVKKDAAYPENPEDIILKM
                     NKMMVQEAFYKDPCLTIEKLCRATHTNRSYLSRAIHRMYGNNFCEWVNLYRIEAAKNI
                     IDKEPGNKIDLEDLAMTCGFNSVRSFCRVFKARESCTPRQYFYQKRK"
     gene            complement(19808..20290)
                     /locus_tag="PLM68_01190"
     CDS             complement(19808..20290)
                     /locus_tag="PLM68_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT54020.1"
                     /translation="MKDTNLVYGGRWVEETQIQDSVACQTLQEKHNNITRVYMDIYQK
                     IKDYLTREKAYKSPAFTRNDMAVGVGTNITYVSRALNECAGVSFKQLINSYRVKHAQE
                     YFRQHPQARLIELCNESGFRSLTALNIAFRENVCMTPGEWCRKELSKINNEKDYEKQE
                     "
     gene            21233..>23104
                     /locus_tag="PLM68_01195"
     CDS             21233..>23104
                     /locus_tag="PLM68_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT54021.1"
                     /translation="MEYNFSDIEQKWQSFWSSHKTFKVEADSSLPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYTRYKRLKGYNVLHPMGYDAFGLPAEQYAIQTGQPPQKTTSQ
                     NIKRYREQLDKLGFSYDWDREIRTCDPSYYKWTQWAFLKMFHHWYDMELDKACPIETL
                     IDAFNTHGNTRIKAAHNCKEEFGASEWKAFSDKEKEDILMSYRIAYIGETTVNWCAAL
                     GTVLANDEVREGFSIRGDYPVVQKKMKQWQLRVSAYAERLLKGLDDIAWSDSLKEMQR
                     NWIGKSEGAQMEFMVKNGSETFSMSVFTTRADTIFGVTFMVLAPESEWVNLLTAPEQK
                     EEVEKYVAYVKTKTERERMSQTHHVTGVFSGSYATNPFTGKEIPVWISEYVMAGYGTG
                     AIMAVPAHDSRDYIFARHFKLPIIPLIEGCDISSESFDAKEGIMCNSGFLDGMTVQEA
                     IPAAIEEVEKRKLGVRTTNYRLRDAIFSRQRYWGEPFPIYYRDGMATPLPESQLPLEL
                     PPLDSFHPSSSGEPPLANAKNWTYGEYPLETSTMPGFAGSSAYYLRYMDPHNSRELVS
                     RDSNNYWQDVDLYIGGTEHATGHLIYSRFWNKFLFDLGIVCKEEPFRKLVNQGMIQGR
                     "
BASE COUNT         6400 a         5350 c         5570 g         5784 t
ORIGIN      
        1 atggaatggt ttaccaagct caatgtgggg aatttatggc ctttgatcct ggtggccgtg
       61 ggtatattga ttttcacaaa agcaagaaag ggcgatcacc actacagcag gtcagggaat
      121 gaatcctggc atgaccgtcc ttcaagcgga tccacccgct ttaataacgg atatgttgat
      181 tattcaatgg ttttcagcgg ttctgacact gtctttcttg aaccggtatt ccgtggaggc
      241 aatattgaaa gtgtatttgg cggagttacc cttgatttgc gaaataccgc actgcaggag
      301 ggagtgtcct atttgaaagt ttcctctgtt ttcgggggtg tcacgttatt catcccaccc
      361 tcctggaata ttgaaattca aagtgattcg gtattcggca acttcaagga caacaggcct
      421 tatactgccg gagtggataa gaattccaaa ttgataataa aagcagaatg cgtttttggc
      481 ggtggtgaga tcaggtaaat acaacataat ggcaactggg agcgttgttc ttgcaatagc
      541 gctcctgttt gcctggctta ccggatctct catttcactt gcatggttcg aaatgctggc
      601 ggatggcttc ataattgctg catgcatgac aggtctgggg ctttatacaa ataaaatgga
      661 tacatacggg atgccccctt atgttcaaag cctcatcttc tgtgtcggga ttgccggcat
      721 acccgtcctg gctatgtacc tgtaccttga tccggacgga tttcgtaatt tcgtacgaat
      781 catcccttta cggatcttta ttgctgccct gatatgcctt gttttattat tcctcaaaaa
      841 agccgggaag gccggcaaaa cagattctcc tctgccggaa aagtccttgt ctgcctcctc
      901 gccctcacag ggataccagg ataatatcat ggtcaggtcc gggaatggta taaaagtaat
      961 tcctgtaaac gatctgctgt atatacaggc cgacggggat tacatctggc ttgttgcaaa
     1021 agacggcaaa tggatgaagg aggaaaccat gaaacacatg caggaaacac tttcacctac
     1081 ccgtttcgtg aggatccacc ggtcctttat tgtgaatgtc ggcgcaatca gccggattga
     1141 acgttacgga caacaacagt tgatcatcct gacaaacggg gactccatca gaataagcac
     1201 ttcgggttac agaacactgc gggatgcttt gggattttga aacctgattt tgttttttgc
     1261 ttacctttgc aaaaaaaaga tggactacaa gatcagggac cttgagcttg ccggctttgg
     1321 taacaaagag attcttctgg cagaaaagga aatgccgggc ctgatggctt tgcgcaggcg
     1381 ttatgcagat aagatacccc taagaggagc gcgtattacc ggaagcctcc atatgaccgt
     1441 acagacagcc gttctgatag aaacactcag gatgctcgga gctgatgtac gctgggcttc
     1501 ctgcaacata ttttctaccc aggaccatgc agccgctgcc atggccgctt ccggagtgcc
     1561 tgtttttgcc tggaaaggag aaaccctgga agaatactgg tggtgtaccc tgcaggcact
     1621 ttcctttccg gatggcaaag gacctcatat ggtagtggac gatggcgggg acgcaacctt
     1681 actgatacat aaaggcgtac ttgccgaaaa agacccttct tccctggata agctcacagg
     1741 aaacgaagaa gaaaaaatca tagctgcctt gttgaggaaa acactgcagg aaaatcccgg
     1801 tatatggaca cgcatggtca gggaaatgaa gggggtgagt gaagaaacca ctacaggagt
     1861 tcaccggtta taccagatgt ttgagaaagg ggacttgctt ttcccggcca tcaatgtgaa
     1921 cgactcggtt accaaatcaa aatttgacaa cttgtacggt tgccatgaat ccctggccga
     1981 tggcatcaag cgtgccaccg atgtgatgat cgccggaaaa gttgtggtgg tgtgtggtta
     2041 tggagacgtg ggcaaaggat gcgccaggag catgcgttca tacggcgcga gggtaatcgt
     2101 tacggaaatt gatcccattt gcgctttgca ggcatccata gaaggatttg aagtccgcaa
     2161 gctggaaaac tgcctggatg aggcacgcat ttttgtaacc gccacaggga attgtgatgt
     2221 gatacgcata gaccatatgg aaaagatgag agatcattcc atcgtatgca atattggcca
     2281 ctttgacaat gagattcagg ttgacaggct tacaaattat cccgggatca gaaaggtcaa
     2341 tatcaagcct caggtcgatc agttcttttt cccggacgga cactccgtaa ttcttctggc
     2401 agaaggtcgc ctggtgaatc tgggctgtgc cacaggacat ccttcttttg tcatgagcaa
     2461 ttcctttaca aaccaggttc tggcacagat caatttatgg accatacaac acgaattggg
     2521 ggtttatctg ctccccaagc atctggatga agaagttgcc cgactgcacc tggattacct
     2581 gggggctgaa cttactccgc ttacaaagaa acaggctgat tatatagggg tgccggtcaa
     2641 tggccctttc aaaccggatt attacagata ttgatcatta tgacgaaatc acgcatcaaa
     2701 attgttgacc tgctcaagat gcaggctgga atccctgtga tggtgaaagg ctgggtccgc
     2761 acaaaaagag gcaacaacaa aatcactttc attgcactga acgatggttc caccattcat
     2821 aacgtacaga tagtatgtga tatgaatgtg ttcacagaag acatgcttcg tccggtgacc
     2881 accggagcct gccttcgggt gagcggacag ctggttgcct caccgggtaa ggaacaggct
     2941 gtcgaaatcc aggcagaaga gattgagatt tatggaacgg cagatgcgac agtctaccct
     3001 ttgcagaaaa aaggacacag catggagttt ctccgggata ttgcccacct caggcccagg
     3061 accaatacat tcggatgcgt gctcaggatt cgtcacgcca tggcatttgc catacataaa
     3121 tacttcaacg acaagggatt cgtatacctt cacactccaa tcatcaccgc ttctgactgt
     3181 gaaggagcag gtgccatgtt ccaggttaca accctggacc tgaacaatct gcctaaagac
     3241 acagacggat atgtcgatta tgcgcaggat tttttcggcc ggcagaccag cctcacggta
     3301 agcggccagc tggaaggaga actcggggcc atggctttgg gcgaagtgta tactttcgga
     3361 cccactttcc gggccgaaaa cagcaatacg cccaggcacc tggctgaatt ctggatgata
     3421 gagcctgaaa tggcctttta cgatatacac gataacatgg acctggctga agattttatc
     3481 aaatacctgg tccgttatgc cctggagcat tgtgcctcag acctgcagtt cctgaacaac
     3541 ataatagaca agagcctgat tgaaaggctg gaaggcgttt acaacataga atttgtgcgt
     3601 cttacctata ccgaagggat tgagatcctg accaacagcg gccagcaatt cgaatatcct
     3661 gtatactggg gggttgacct gcagagtgaa cacgaaagat acctggtgga aaagcatttc
     3721 aaacgtccgg tgatcctgac cgactatccc agggatatca aagctttcta tatgaaagaa
     3781 aacccggatg gaaagacagt acgggccatg gatattcttt tccccaagat tggagaaatc
     3841 attggtggat cgcaaaggga agagaacctg gaagttcttg tgcggaaaat gaaggaaatg
     3901 catgtatcgc aagagaacct tgactggtac ctggatacgc gccgttttgg aacaggtccc
     3961 cactccggat tcggactggg ttttgaaaga ctgctcctct ttataaccgg tatgacaaat
     4021 atccgggatg tgattccttt tccgcgaaca cctaaatctg cagatttttg atcatgttgg
     4081 taattggcat agccggagga accggttccg gaaaaacaac ggtggtaaac aagatcctgg
     4141 aacgtcttga cgaaaggcaa attacagtaa ttccacaaga ctcctattac aaagacaatt
     4201 cacacctgcc actggaaaaa agacaggaat tgaactttga ccatccgcag tccatagact
     4261 gggaactgat ggtggaccac ctgctgaagc tgaaatcagg atatgcgatt gaacaaccca
     4321 tctactcata cataacctgc acgcgttcag cagaaaccat ttatacaaaa gccacacaga
     4381 tcatcatcgt ggaagggatc ctgatcttca cggaccctcc cctgagaaac tgcctggata
     4441 tgaagatatt tgtcgacgcc gatgccgatg acaggttgtc ccgcgtcatt tcgagagata
     4501 tcattgaaag gggtcgttcg gtgaacaagg ttttggagcg gtacgaaaaa accgtaaagc
     4561 ctatgcatct gcaattcatt gaacccacca agcggtatgc cgacatcatc atcccacagg
     4621 gggggcacaa cactgtggcc atcaatatgc tcatatccac cattgagaaa gccctcctcc
     4681 cgagttaagc attcccggtc atgaaacagg aatcaagaat cggactttac cttaccatca
     4741 tcatacacct ggttgcattg attgttgttt tatcgcttca ggtctcttct ttcattcaaa
     4801 aagagtccac ctatatcctt gatttcacac cacaggaaga actggaaatg gagcaacaga
     4861 gggaaaggat cagggccgag gtaagcgaag gtctggatga cctgattgcg ggtattcaaa
     4921 aggagtcaag aaacgttgcg gttgatgcaa ccctgagaaa ccgtcccctg agggatgacc
     4981 gtcatgcgga tccctcccag gtttacaaag aggcggagga gttgcagaaa cgcctggatg
     5041 ctgcccgcca ggagatcgag gagcagggtg gcatggaaga aatttccatg acagagaaag
     5101 aggaaaacga ccaggaaacc atcccttata cgggccctgc cgtgctggaa tataccctgg
     5161 aaggacgcaa ggccatgagc cttcccatac ctgtatataa atgtctggga ggaggtgatg
     5221 ttaccgtctc tatccaggtg gacaggaaag gttatgttca gaaagccgct attgttgcgt
     5281 atttttcttc ccaggaccgt tgcctgcagg agtatgccct gcgtgctgcg cgtcaaagcc
     5341 gttttaccgc ttcggtagat gccccgcccc tgcaaacggg ggaaattgtg taccgtttta
     5401 ttcctcaata agcgttttat gtttattttt gaaccatgga cgaaagagaa taccgggaaa
     5461 taaaagaatt gctcgcgcag ctaaccgcca ggatcgatgc tttatacacc ccggctccgg
     5521 ctccggttcc ggttcctgac acacccggtt attttgaact ggaaacccct tcggtccctg
     5581 tttcccctac ccttaaggat aagttttcag gattggaccg gcgacccacg cttgcggact
     5641 cgctggcaaa aacaaagaac aaggggctcc tgaaagaagt cattccgctc aacgataaat
     5701 atctttatct gaaaacactt ttctctgaag actccggttt gtacgaaaaa accattgccg
     5761 acctgcagtc tttcaaagat atgcaacagg ccgaagccta cctggcgcaa cattttccct
     5821 cctgggaatt atctaccccc gccgtgcaga aatttctcac ccagctggaa aatgtgttct
     5881 aaactgatca tagtacccac acccataggg aacctggaag atatcacact gcgtgcgctg
     5941 aaggtgctgc aggaagctga ccttatcctg gcagaagata cacgcaccag cagtgttctg
     6001 ttgaaaaaat atgctatcaa gacgcatatg gtgtcattcc accaatacaa cgaacacaag
     6061 caggttgccc cggtcctgga gaagattcgc cagggaaagc tcgtggcgct gataagcgat
     6121 gcagggacgc ccggtatctc tgaccccgga ttcctcctgg ccagggaatg cctggaggaa
     6181 gggatccccg tagaatgcct gcccggacct actgcgctga tcccggcatt gataaacagc
     6241 ggttttcctg cagaccgttt ctgttttgaa ggattcctcc ctgtcaggaa gggaaggcga
     6301 agccgtatgg aagtactggc tgccgaaaca cgtactgtga ttttgtatga atctcctttc
     6361 aggctggtaa aaaccctgca gcaattggag cagtacctgg gacgggatcg tatggcctgt
     6421 gtttgcaggg aattgacaaa aatacaccag gaaacaagac ggggcacttt agcagaactc
     6481 agtcaatggt acgggcaaaa tactcctaaa ggtgaaattg taattgtgtt gaaaggtaga
     6541 ggaaaggatc atgaaacgga gcaataggca tcattatatc attcatgcca cgggaattgc
     6601 cggaattatc attatattag taatcaaaat tttctgggaa cacctgcttc ccggccatca
     6661 gacaggccgg gattcacgga tgtcagaaga aatgctgaac ctgtatgcag agccaccggc
     6721 aacagcatcc gggaagagca ctgttccttc cggtcagtgg acatccgcaa cagcctcccg
     6781 ggaaaaacct ataaccggca gacaggaaaa tcctgttcct gcgcccgggg aaaggcctgc
     6841 gaccggccgg cagtcagaaa accatcctga ccgtggcatg gaatcagccg cggaaccagg
     6901 gaagtcctcg cccggtaaat acaatccggc aactcacatg aaaattgaac tgaatacggc
     6961 agatagtgcg gaactggtaa aattatacgg gattggcccg tattatgctg tcagcatcat
     7021 gaaatacagg gaaaaactgg gtggctttgc catccctgag cagctgctgg aagtaaaggg
     7081 catggacagc gaaagactgg aaggattcta tgaccgtgtt tttgcagata catctttcat
     7141 ttcaaaaatg gatctgaaga cagccactga agaccagctg gcacagcacc tgtacatagg
     7201 caggtacctg gccaggtgta ttgtgagata ccgggaaaca gccggagtgg attcctgtac
     7261 cctggagcac ctggtaaggg acaaagtgct taccggagaa caggcagtca aaatcgggtg
     7321 gtatctttac tgagtggtgt tttttcctat atttgcctgt tataaaaaaa tctcaaacca
     7381 atatttgcta tatgaatata ctggaaacca aaaatgtggt gaaaagtttt gacgggctcc
     7441 tggcgctgga tgctgtgagc atagcagtcc cccgaggatt ggtctttgga ctgttggggc
     7501 cgaacggagc cggaaaaacc acccttttga ggattgtgaa tcagatcacc gcacctgact
     7561 cgggaagcat attttttgat ggacaccccc tggcggcaga agatgtcttc agcataggat
     7621 atcttcctga agaaaggggc ctgtacaaaa agatgaaaat tggcgatcag gccctttatc
     7681 ttgccaaact caagggcatg acacattcgg aggcgcttgc cagcctgaaa tcctggttca
     7741 tccgctttgg aatacaggcc tggtggaata agaagatcac cgaactgtca aagggaatgg
     7801 cccagaaagt gcaatttgta accacagtga tgcacaatcc gaaacttctc atcctggatg
     7861 agcctttcag cggttttgat cccattaacg tagatcttat ccggaatgaa atccttcggc
     7921 tgaaggaaaa cggcacctct gtcattcttt ccacccataa tatggaatct gtggaagaaa
     7981 tgtgcgacca gattgcgctg atagacaatg cccgccttat ggttaccggt aatcttcatg
     8041 aaatccgccg catacacgga gacaaccata ttgaaattct ctaccgggct tccgaaccca
     8101 ttaatccgct caaccttggt gtcgtgcctt tcaatatcgt ttcccagggt aagaaagacg
     8161 gatcctacca gatggtggtt gatccggcag ataaaaatga caccaatgcc gtgctccgct
     8221 cactcatgcc tcatgtaagt gttgttggca tgaccgaaat catcccgcgt atgcatgaca
     8281 ttttcatcaa acttgtaacg aataaggagg aaagaaaatg aatatcaaaa agataaacct
     8341 cataatcagc agggagttcg ctattcgtgt aagaaagaaa tcttttatca ttgccacttt
     8401 tctaacccct ttgatgatgg ctgccctcat taccgttccc atgatcatgt caggagtcag
     8461 ggatgacagg gaaaaagtca ttgaagtgat agatccctcg ggtattggac aagctgtgct
     8521 tgaaaatcag gagtatatca ctttcaactt ccgccaggag ggcgatctgg aagcttaccg
     8581 ggcaggtttt gaagacaaga atctccatgc cgtgtgtgtc cttgaggaca ccggtaacaa
     8641 ttcggttgat ataagactct acagtaccgg tcagatagac tctgatgtcc agcgatatat
     8701 ctcgcgccag gtcagccagg aagtggaaag acgcaaactc aacacgtacg atatccccca
     8761 actgcaggaa atcatgcagg atgtgaggac caacatacag gcaaagacct ttacatggac
     8821 agacacagga gacgagaagg aaaccatcaa ggaggtgtat atggccatcg cctacctgag
     8881 cagctttttg atctatatat tcattttcat gtacggatcc atggtcatac ggggagtcat
     8941 cgaggaaaag acgaaccgta tcgtggaagt catcatttca tcggtcaagc cggtggaact
     9001 catgctggga aagattgtgg gtattgcctt ggtagcctta ttgcagttcc tgctatggat
     9061 actgcttact gtaattatcc tgctcatagt catggccatg gtcggaacag ggacccttgg
     9121 cgagatggga caggcagcac ctcagatcca gggcggactg gccgggacca tcgctgcttt
     9181 tgacacaggg aacggtgtac ttacaggcat tgcaagcact ttgtcacaaa tcaatgccgg
     9241 aaaattcctc ttgagtttta tgctctattt tatagggggt tacctgatgt atgcgtcgat
     9301 gttcgcggcc ataggctcag tttccgatgc cgagaccgac acgcaacaat tccagctgcc
     9361 catcaccata cccctgatcc tgggtctgtt catcatgatg catacattcc aacaccccga
     9421 cagttccctg tccttttggg cttccatgat cccattcact tcgcccatgg tcatgctcgc
     9481 acgccttcct tttggagggg taccgctatg gcagctgctt ctttccatca cccttctctt
     9541 tggcactttc ctgggcgtgg tgcatttttc ggccaaaatc tacaaggtgg gtatacttat
     9601 gtatgggaaa aaagtaacct ggaaagaatt atggaaatgg atgcgctaca aaaacggctg
     9661 ataagaattg tcttcttatc tgccctgctc ctgattgcca ggatgaccac ctccctcgcc
     9721 cagacgatca atgcaaccag ggtactgatg gacacctcgg cttaccaggc caaggcttcc
     9781 aaagctgtca agcaaatcat tgacacctac tccccggaac tcaaccggaa gatgggagag
     9841 gtggttggct acaccacgat ggccatggac tcctaccctc ccgagagccc gctgtccaat
     9901 atggcagccg atgccctgct ggccatcgca aaaaggcatt atggcctgga taaggtggat
     9961 tttgccctta ccaatttcgg aggcatacgc acctcttttc ccaagggaga tttgagcctt
    10021 tactatgttt atgctgcctt cccgttcgag aattcgcttg ttctggtagc cctgaaagga
    10081 acccaggtaa aggcattgtt cgagattttc gcccgcagcc atgccgaggc cctggccggt
    10141 gtccggttcc gggctgataa ccgcaaaatt accaaattgc tcatcaatgg ccttcccctt
    10201 gacgaggacc gtgtgtatta tctggccacc attgatttcc tgctttcagg aggagacagg
    10261 atgaccgccc tgagggagaa cctgggggtt gattattccg gcataacact cagggatgtc
    10321 atcctggagt atatcggtgt atttacaaaa aacggagagc ccatacccgc caggaaagat
    10381 ggacgggtgg tgatcaacaa cccgcgtgaa taatgaaacg attttttacc atagtacttt
    10441 cccttgtgct gctcgctgcc atttcacggc ccctttccgg ccaggatctt gtaattgtgc
    10501 ataccaatga ttttcacagc cagatcgagc cctttcaatc gggtcgcaac gcaggcacag
    10561 ccggcttact gagcttgtcg ggatacctgg aggaatcaag gatggtttgc ccggacctgc
    10621 tctaccttga tgccggggac tacaatcagg gaacaccgta tttcaacctt tttggcggac
    10681 aggtagaagt ggaaggcatg aatgcactcg gactttttgc caccacgctg ggaaaccatg
    10741 agtttgacaa tggcatggaa gatctggtaa ggcgtcttaa aaaagctgat taccacgttc
    10801 tatgtgctaa ttacgatatt caacataaac cgctcaggag gttggtcaaa ccatatgtca
    10861 taacacgggt aaacgggaaa aaaattggca tcataggcct gacgcttgat ctgaatggtc
    10921 tggtttcctc agaggtgctc aggaaaatga actacaggga cgtaatcctg acagcaaacc
    10981 gtatggcgtc attactcaaa agaaaaggtt gtgatctgat aatctgtctg acccaccagg
    11041 gtcttgactc agatatggaa atggctgctc aatcaaggaa tataaacctg atcatagggg
    11101 gacattccca tacttttctt gaagaacccg tgaaaaaagc agacctggac ggacaggaag
    11161 tagtgattgt acaggcaggt gcttacgggg tttatgcagg acgtattgat ataacatttt
    11221 aagcgggcga catcatgttt ctggaaactc aaaaatggaa cggcaggata gaagttatct
    11281 gcgggtccat gttctcgggc aaaacggaag aactgcttcg caggctcaaa agggccaggt
    11341 ttgccaatca gcgggtggaa atattcaaac cccggataga tgtgaggtat tccacggatg
    11401 atgtggtatc tcatgacagc cattcctatc cctcaacacc ggtggactcg gcagaaagta
    11461 tccttttgct ggccacaaac gtggatgtgg tggccattga cgaagcacaa ttctttgacc
    11521 agaaccttgt cagggtctgc cagatactgg ccaacaacgg tatccgtgtc attgttgccg
    11581 gtctggatat ggattaccag ggaaatcctt tcgggcccat gccgcaattg atggctgtag
    11641 ccgagcatat tgtcaaggtg catgccatct gcgtgcgttg cggggatccc gctcagtatt
    11701 cccatcgtct tagtgacagc acccggcttg tggagcttgg ggaaaaagac atttacgaac
    11761 ctttatgcag acattgtttc aaccagatca agaaatgaac gataatcaat tcacacacct
    11821 cgaaatcaac attcccaaca tccgccacaa ccttaattat ttccgttcgt ttctaaataa
    11881 aaagacgaag cttcttgttt tggtcaaagc atacggatac ggtcacggta tcggggagtt
    11941 ttcccgcatc ctggaagaga atggtgtaga ttacctggcc gttgcatttc ccggcgaagg
    12001 cgttgccatt cgcgatgcag gcgtaaagct tcctgtcatc gtgctttctg ccggcatgga
    12061 gcactattcc ctgctgatta aaaaccagtt ggagccaagc ctcccctgcc tggaagcttt
    12121 caggcgtttt tcggaagaag cggcagtcat gggagagaag gggtatcctg ctcatattaa
    12181 actcgacacg ggcatgcacc gcctgggatt tgaagaggaa gagctccgtt tcctggcgga
    12241 tgaattgcct ttgacgcaat cagtgagaat cgcatccatt ttttctcatt ttgtggcctc
    12301 gggggatccc cggcatgatg atttcaccag aacacagata gcccgctttg agtctctgtc
    12361 ttcttttttg atgcagcatc tggattaccg gcccatccgt cacctgctca attcaccggg
    12421 tatagaacgg tttgccaaag aagccgaata tgatatggtc cggctgggta taggaattta
    12481 cggaatgtcc tatgtggaca gaagcaggct ccgcccgaca gcctatctga aaactccggt
    12541 tttgcaggtt aaaacggtag ctcagggtga aacagtgggg tatgccagag cgggggtggt
    12601 aacggccccg ggaacccaca tcgccactct ggcaatcggt tatgcagacg gggtgaaccg
    12661 tcatctgagt ggtggcaaag cctgcttctc tgtgaacggc cacagggctc cgaccatagg
    12721 taatatctgt atggacatgt gcatgattga tgtaaccggt attgatgtca atccaggtga
    12781 tatggttaca gtttttggtg acgaccccac catatttgaa ctggctgaaa ttttgggcac
    12841 cataccgtat gaaatcctta cttccgttgc gcatcgagtg ccccggatct atgtggaatg
    12901 atcaggccat catcttgcag accatttcca tgagcagttc tccgtccgag gcttctccgc
    12961 ggtcagaaga cttgtacttg ctgtcataga agcaaagcag ttccatggct ctgaccgtac
    13021 gcctgaaggt aaaaaaacgt gacgcctgga taatttcttt gaaaaagtag taattgcccc
    13081 ccatgagttt tcctatgcct tcttgtgaca gggacggatt ttccatgcgg agggcgtggt
    13141 accggagtat ccgggacaag tggacataca ataggctgat tatcataacc aggggatact
    13201 tcttgtcatt ctccgaaaag tattgcacta tgggctgcac cccggcaaaa ttcccgtctg
    13261 tgaggtgttt gaacatggta aaagggctga aatcacggtt aatgcccaca gaattttcaa
    13321 ccatgtctgt ggtaatggcc caggttcctt caggtatgag aatgaataat ttgtccagtt
    13381 ccatcacgac ccgttgcagg tctgtcccca ggaaatctgc cagaagagca gctgcctgag
    13441 gttcgatact tgttttcttt tctttcaggt aagtagttat ccactcctgc atctggtagt
    13501 ccctaaaggg taccgactcc aggatttcac aatatttttg tgccgttttc cagaactctg
    13561 ttcttttgtc tgcagatttg cccataaagc agagaaccag gacacatgaa gggttcatgg
    13621 cctttaagta cgataccagt ccatccggtc ccttgagctg ctgtgcctcc ttgacgatga
    13681 taacctgcct tgaagccatg accggatacc ttctggcatt atctgcaatc tgcataacgg
    13741 tagcatcctg tccatagatt accatctggt tgaatgcctt ttcatcttct gtcagtgcat
    13801 tttcggcaat aaaggatgtg attttatcca cataataggg ctcttccccc atgagcaggt
    13861 aacagggttt atatctcccc tcttccaggt ccttcatgat ccgcttaaag gtagtgatgg
    13921 tttctttgga tgtcgtttta gccatatgac aaaattacat aaatttgcac aatgaacagc
    13981 ccctctgcca tatattgcgc cgtaaggaaa aaatgggtgg aacgcacacc ggaagaggaa
    14041 gtccgtcagc atatcataaa acacctgaat attgtttgcc aggcacccct tcacctgatg
    14101 acctgtgaat atcccgtcca gcatcttaac cagcgtgcag acctggttct ccacgaccgg
    14161 gaagggaaac cgcttgtgct ggctgaattc aaagctcccg ccgtcagggt ggatgacaag
    14221 accatagaac agctgagaag gtacaatgaa gaaatcatgg cgccgtacct tcttatcagc
    14281 aacggttcac agacattctt ctggaagttg gaccgggtga acggctcgta ccaatccatg
    14341 aaagatatac ctccatacca ggatctgatc ataaaaaacc ctcactgatg aaagatcccc
    14401 tgaaacccgg aattttcaaa atcgtatccc gccaggctgc tgctaggaat gtacgtgcct
    14461 atgtaatagg cggatatgtc cgtgatcatt tcctgaaacg tccctgcact gatattgata
    14521 ttgtggtgga aggcaatgga atggaacttg catctgacgt ggcttccgta ctaaaggtaa
    14581 atgctacact gtttaagaat tttggtacag ccatgttgcg ctaccgcgga ttggaaattg
    14641 aattcgtcgg ggccagaaag gaatcttaca gtactgattc aaggaaacct gttgtggaaa
    14701 acgggaccgt ggaagatgac cagaaaaggc gtgacttcac tattaacgct atggcattca
    14761 gcctgcagca ggaggattac ggatccctta ccgacccgtt caatgggctt aatgatctgc
    14821 atgataaggt tatacgtact cctttagatc cggacaagac tttcagcgat gatcctctga
    14881 gaatgatccg ggccatccgg tttgcagctc agctggagtt caccattgac aatacagcat
    14941 tcaattccat acaaaggaat gcagacagga ttcagattgt ttcccaggaa aggatcacta
    15001 ctgaattgca aaagatcatg gcctcccgga aaccttccgc cggtttttac ctgttggaaa
    15061 aaagcgggct tcttgccatc atccttccac aggtagcact gttgcagggt gtggaaacca
    15121 tggaaggaaa aggtcataag gaaaatttca gtcacaccat ggaagtgctc gacaacgttg
    15181 catccatgac tgacaatatc tggcttcgct gggcagccct gttacacgat attggcaaac
    15241 cttccaccag gcactttgat aaggagacgg ggtggacttt tcacggtcat gatttcgtcg
    15301 ggagtaaaat gatacccggc attttccgtc aactcagatt gccactgggt gaggaaatga
    15361 aatacgtaag aaaattggtt cagctccact tgagacctat agcccttgtc aacaatgtga
    15421 gtgattccgg ggcgcgcagg cttttgttcg aggcagggga agacattgat gacctgatgt
    15481 tgctgtgccg agcagatatt acatcaaaaa accccatgac ggtgagacat catctcggta
    15541 attttgaaca ggtaagtaaa aagctcatcc aggtcgagga aaaggatgct atccggaatt
    15601 tccagccccc catctcggga gagataatca tgaagacata caaccttcct ccctgccggg
    15661 aagtaggtct gatcaaagat catatcaagg aggctatcct ggatggaaaa atcggcaaca
    15721 attatgagga ggcactggac ctgatgaaac aaagggcggc agaactggga atcagtgaag
    15781 caggtgcatg agttcttccc tggtacgcat atccttcagg aatgttcctg tgaaggccga
    15841 ggtcaccatt tttgaatgct gtttctgcac accgcgtatc tgcatgcaca tatgctcggc
    15901 ttcaatcaca acggctacac ccatggggtt gagagtctcc tgaatacaat cgcggatctg
    15961 tactgttaag cgctcctgta cctggaatct ccgggcaaat gtctccacaa ccctggttat
    16021 cttgctgatc ccggtgataa agccatttgg aatgtaagcc acatgggcct ttccataaaa
    16081 aggaagcata tggtgttcac acagggaata catttctata tcccttataa ggaccatttg
    16141 ctggtattct tcacggaact tggctgagtt cagaatggca gccggatctt ccaggtatcc
    16201 tttggttaaa aactgcatgg cttgtgctac cctcagcggg gtcctgagca gtccttccct
    16261 ttctgtatcc tctcccagca gttgtagtgc ttccttgtaa agttcttcca gttttctagt
    16321 cgtttcgggt gtaaaatgat cgttgttggt ataagacatg ggtaaacgga tatggattta
    16381 aaaaacaaaa gtacaaaaaa aaccaacccc gcaaaaacct taaactttca gaacctgcag
    16441 tagtcaaatg gaaaatcaag gtgtagacta atcatatttt ttgtacattc gcaaccatgg
    16501 aaaaagaagt acttatcaag atcaggggaa ttaagaaaat ttacaagatg ggaacccagg
    16561 aagtgcgtgc tctgaacggt atagacctgg aaatccggaa gaacgaatat gtggcgatca
    16621 tgggtccgtc gggatccggt aaatccacat tgatgaattt gctgggatgt ctggatacgc
    16681 ctactgaagg aacgtatatc ctgaacggaa cagatgttag ccagatgcgg gacgaccagc
    16741 tggcagaagt aagaaacaag gaaatcggat tcgttttcca atcatttaac ctgctaccca
    16801 ggtacaatgc cctcaataat gttgcattac cattgatata cgcaggtttg ccacggcagg
    16861 aaagggagcg ggaggctaaa aaagcccttg tcaacgttga tcttgaagac aggatggacc
    16921 acaagcccaa cgagttatcg ggaggacaaa aacaaagggt agcccttgcc cgggccatgg
    16981 tcaacaatcc gtcaatcata ctggccgacg agccgactgg taatctggat accaaaacat
    17041 ccctggacat catggcactc tttgaacaag tatacgccaa gggaaacacc ataattgttg
    17101 ttacccatga ggaagacatt gcccgttatg cacggcgggt ggttcgcctg agagacggac
    17161 ttgtagaaag tgacgagatt aacgccaatc cggcgcttgg caattcatga gcaagatata
    17221 cacaggcaac ggagacaacg gatccacttc actccaggac gggagcaggg tatccaaggc
    17281 tcacccgcgt gtggcagcat atgggaaagc agatgagctc atatcctgga tcggacttat
    17341 ccgatcccat ccggatttct cctctgacgg ggaattgcat gaaatcaatc attttttgcg
    17401 aaaaatccag gtgggtctga tgtatctggc tagaagactg gcttcgaatg attcatcccg
    17461 gcttttttat tacgattttg aaaccgccac caaagccctg gaagaatcga tagacatcat
    17521 gcaggcacgt ctgcctgcca tccattcttt catacttccc tacaagccgg taatttcatc
    17581 acatattcat atagcccgta ccatctgtcg tgagacagaa cgttatatgg tcgtccttgg
    17641 cacggatatt gccagcgcag atatgctcac atacataaac cgtctttcag actacctttt
    17701 tgtactggca cggttcatga ccgttaccaa taaatgtgaa gaagataatt ttgtgtaact
    17761 ttgttcactt tttttaaaat ttcaacgatg tactggacac ttgaattggc atcaaaactt
    17821 gaagacgctc catggccagc taccaaggag gagcttattg attatgccac acgctcgggc
    17881 gctccgctgg aagttattga aaacctgtca gacctggagg acgatggtga aatttatgat
    17941 agtatagaag acatctggcc tgactatccc agcaaggaag atttcttttt caacgaagat
    18001 gaatattaaa atcattgaat gaacataatt attgccggag ccggagaggt cggttttcat
    18061 ctggcaaaaa tgttgtccga tgaaaaccac cagatcacaa tagttgataa aaatgaagcc
    18121 cgcctgaacc agatagcaga gacttcagat gtggttcctg tggccggaat cgtgacatcc
    18181 atatctaccc tggaaaaagc aggaatcagc cgttgtgatt tattcattgc cgtaagtcct
    18241 gccgaagaac aggatatgaa cattgtggca gccctgctgg ctaaacaact gggagccaaa
    18301 cgcgtgactg cccgtatcaa taacaatgag tacctgaaac aagagaacag ggtcctgttt
    18361 accgaaatgg gtattgacct gctgttttat cctgaaaata ttgcagcaca tgaaatcgtt
    18421 gacctgctga aacaatcagg cacttcagaa ttcatggatt tctccggagg caggttgcag
    18481 ctgatagtgc tgaaactgca ggaaagttcg tccatagtaa ataaaagcct gaaacaggtg
    18541 gccgaagaaa acgggggaaa tattcctttc cgggcagtag ccattgcgcg tgaaggcaat
    18601 acaataattc cccgtaaaga cactgttttt cagttacatg accttgtttt tgtcattacc
    18661 aaaagagaag ccgcccatgc agcaatggtg cttaccggga aagaaaacat ggtggtaaga
    18721 aaccttatga tcatgggggg cggcagggtt ggtgaaatgg ttgcacatca aatggaatcc
    18781 gctattgaaa taatacggat tgttgaaatt gacagcaacc gctgtcgtga attaaccgtc
    18841 tccctgccac gttccctgat cattaatggc gatgcccgca atacagacct cctgctggag
    18901 gaagatctta cgcaaatgga tgcctttgtg gctgttactt ccagtgccga agccaatatc
    18961 ctttcctgta tggctgccaa aagggcggga gtcaaaaaag ccatagctca ggtcgagaat
    19021 cttgattata tcaagctggc ggaaagcgtg ggagtagatg ccacaatcaa taaaaaactc
    19081 ataacggcaa gcaggatatt tcgtttcacc atgacaggcg atgtgcccac catgaaatgc
    19141 ctgaatggat ccgatgccga agttatggaa ttcatagcca aaccccgcag tctggtaacg
    19201 cagaaaatag taagggacct tggcttcccc cgtgacgcta tcataggagg tataaccagg
    19261 ggaaacacca gcctgattgt acgtggagac accgaaatca aaccatacga cagggtggtt
    19321 gtcttctccc ttccttccgc gctgccgact attcatagat ttttcgtata aaagctactt
    19381 gcgcttttgg taaaaatatt gacggggcgt acaggactcc ctggctttga ataccctgca
    19441 aaaagagcga actgaattga agccgcatgt cattgcaagg tcctccaggt caatcttatt
    19501 gccgggctct ttatctatta tgtttttggc tgcttctatc ctgtataaat taacccattc
    19561 gcaaaaattg ttaccataca tacggtgaat agccctggaa aggtaggacc ggttagtgtg
    19621 tgttgcccgg cacaacttct ctatggtaag acacggatct ttataaaacg cttcctgaac
    19681 catcatttta ttcattttta gaatgatgtc ttccggattt tccgggtatg ccgcatcttt
    19741 tttaacgaac aatacgggat tggtcaaaag ccagccccgt gtacacctgg tgactctttt
    19801 tattaaatta ttcttgtttt tcataatctt tctcattatt gattttactg agttccttcc
    19861 tgcaccactc tccaggcgtc atgcacacat tctcccggaa agcaatgttt aatgctgtca
    19921 gggacctgaa gcccgattcg ttacataatt ctatcaggcg tgcctgggga tgttgccgga
    19981 aatactcctg ggcatgcttt acccggtagg aattgattaa ctgtttaaaa ctcacgccgg
    20041 cacattcgtt caatgccctt gaaacgtaag tgatgttggt ccccacacct acggccatgt
    20101 cgtttctggt gaaagcaggt gatttatatg ccttttccct ggtcagatag tcttttattt
    20161 tttgatagat gtccatatag accctggtaa tgttattgtg tttttcctga agcgtttgac
    20221 aagctacgct gtcctggatc tgagtttctt ccacccatct tccgccataa acaaggtttg
    20281 tatctttcat gataattgcc gtgatgaaaa aaatatgtgc tggtacgaat aaggcaaaaa
    20341 atatccttgc aggccatatg aaacctaaac ctaccagaag gtatgcagcc agagaaataa
    20401 ccggcagtgt aacaatcagc aaccagaatg aacgtatctc tttttgtccc agttcaaatt
    20461 gatatttcaa ttccttattg aggacgaaaa attgcatgaa ccccgataag atgcaggtta
    20521 gacacaacgc actggcaaaa ccggaaaaca ggacacctgt tgtaaattct ttctggataa
    20581 gtactgccaa gagaatgaat aatgcatata tcccgggtag aaacatcaag cttcttttca
    20641 ataaaagatt tctgagattt tttgaggcaa tggatgtgaa tgtaatacag taaatcggaa
    20701 ggcatgtgaa tgtaattctc agataatgat cttctttaag atagataccc ggaattccct
    20761 gcccccggta aaaaaagatt accctcagta aataaagtgc acccaaagcc aataaagaat
    20821 aaatcaggca cctgaaggga atagaaggtg aaaaatgaag ggcagcttta accctgatct
    20881 ttgccaataa aagagaggct gccacagcat aaacaattgt ggccattaca tttaatataa
    20941 acgctaacat agatagtgtg acttgatgaa tgaaaaaata atatatggtt tttatcaaat
    21001 ttacaactct attctctaac gaaaaaggat ttgccggtat aattgggaaa aaaatattgt
    21061 ccgaaaaaaa tgtcctttgt ataacatttg taattaaaag tttacatgat tacatgtgga
    21121 taatccacta aaaaaatata tacgttttgc tacttccctg cacctttttt ccggccttcc
    21181 cctgactaag gttttttatt acctttgtgc ctttacaaat gacaggaaca agatggagta
    21241 taatttttct gatattgaac agaaatggca atctttctgg tcttcccaca agaccttcaa
    21301 ggtagaggct gattcctcat taccaaagtt ttatgtgctg gatatgtttc cctatccttc
    21361 aggagcaggc ctgcatgtgg gccatccact gggctatatt gccagcgata tttatacacg
    21421 ctataaaaga ctgaagggtt acaacgtttt gcatccaatg ggttatgatg cattcggact
    21481 gcctgcagag cagtacgcca tacagacagg acaacctcca cagaaaacga cgtcacagaa
    21541 catcaaacga tatcgcgaac aactggataa gcttggtttt tcctacgact gggacaggga
    21601 aatacgaacc tgtgacccat cctattataa atggacacaa tgggcattcc tgaaaatgtt
    21661 ccaccactgg tacgacatgg agctggataa agcctgtcct attgaaacac tcattgatgc
    21721 tttcaatacc catggcaaca cccggataaa ggctgcccac aattgtaaag aagagtttgg
    21781 cgcctcagag tggaaagcct tttcagataa ggaaaaagaa gatatactga tgagctatcg
    21841 catagcctat attggagaaa caacagtaaa ctggtgtgcc gcgctgggaa cggtgctggc
    21901 aaacgacgaa gtcagggaag gtttttccat tcgtggtgat tatccggtag tgcagaaaaa
    21961 aatgaaacaa tggcaactgc gggtttccgc ctacgcagaa agattgctca agggactgga
    22021 tgacattgcc tggtcagatt ccctgaaaga gatgcagcgc aactggatag gcaaatcaga
    22081 aggggcccag atggagttca tggtaaaaaa cggttccgag actttttcca tgtccgtttt
    22141 tacgacaaga gccgatacca ttttcggtgt cactttcatg gtgctcgccc ctgaaagcga
    22201 atgggtcaac ctgcttacag ctcctgaaca gaaggaagag gtagaaaaat acgttgccta
    22261 tgttaaaacc aagactgaaa gggaacgcat gagccagaca caccatgtta ccggtgtctt
    22321 cagcggctca tatgcaacca atccgtttac aggaaaagag atacccgttt ggatcagtga
    22381 atatgtgatg gccggttacg gaaccggggc tatcatggcg gttccggcac atgacagccg
    22441 cgattacatc tttgcgaggc atttcaagct tcccatcata cctctgatag aaggttgcga
    22501 tataagcagt gaaagctttg atgccaaaga aggcatcatg tgcaattcgg gctttctgga
    22561 cggaatgact gtccaggagg caatacctgc agctatagaa gaagtggaaa aaagaaaatt
    22621 gggtgtacgt acaaccaatt accgtttacg tgacgctatc ttctcccgtc aacgctattg
    22681 gggagaaccc ttccccatat attacaggga tggtatggct acccctcttc ccgaatcaca
    22741 attacccctg gaattacctc cgctggattc tttccatcct tcttcaagtg gtgagccacc
    22801 cctggctaac gcaaaaaact ggacatatgg ggaataccct ttggaaacaa gcactatgcc
    22861 cggatttgca ggcagttctg cctattattt acggtacatg gacccgcata attccaggga
    22921 actggtttcc agggacagca acaattactg gcaggatgtg gacctgtaca taggaggtac
    22981 agaacacgcc acaggccacc tcatctattc caggttttgg aacaagttct tatttgacct
    23041 gggaatcgtc tgcaaagagg aaccttttcg caaactggtg aaccagggta tgatacaggg
    23101 acgt
//
LOCUS       DAOSOF010000010        22696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2285_length_22696_cov_7.147122, whole genome shotgun sequence.
ACCESSION   DAOSOF010000010 DAOSOF010000000
VERSION     DAOSOF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_22696_cov_7.147122"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..449)
                     /gene="gcvH"
                     /locus_tag="PLM68_01200"
     CDS             complement(72..449)
                     /gene="gcvH"
                     /locus_tag="PLM68_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT54022.1"
                     /translation="MNIPANLKFSKEHEWLKVEGDLAIVGITDFAQSQLGDIVFVDIQ
                     TLGETLERGEVFGAIEAVKTVADAFMPVSGTIEEVNESLEDAPQTVNEDPYGKGWMVK
                     IRLINPGEISDLLDAQAYGDLIS"
     gene            complement(499..1089)
                     /gene="ruvA"
                     /locus_tag="PLM68_01205"
     CDS             complement(499..1089)
                     /gene="ruvA"
                     /locus_tag="PLM68_01205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT54023.1"
                     /translation="MYEYIKGTLIAADPAQAVVETGGIGYHLLISLQTYSAIHERKDV
                     LLWIHLQVRDDAHLLYGFATRDEREIFRLLIGVTGVGPNTARMVLSAMSNAELRNAIV
                     TGDVVRIKSIKGIGTKTAERMIIDLRDKMMKIGLDPDAQGTQAPPVSATGVEASAALQ
                     QLGFQRSAVEKVISRLQKENPSFGLEDLIRQALKLL"
     gene            1139..2386
                     /locus_tag="PLM68_01210"
     CDS             1139..2386
                     /locus_tag="PLM68_01210"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HOT54024.1"
                     /translation="MNLSQIREQFPALSQKVYGKPLVYLDNGATSQKPRQVLDVLQEM
                     QGGLNGNIHRAVHYLSVQSTERYDRARATIRDFINAASVNEVIFTSGTTGSLNLLAFS
                     FGQTYIRPGDRIVITRAEHHSNIVPWQMMCKRTGASLEVWEVEESGAMDPKKLEQILD
                     KQGRKPKLLAIAHISNVLGLVNPLREIIRIAHYYGVPVVVDGAQGIVHKKVDVQDMDA
                     DFYVFSGHKLYGPTGTGVLYGKEKYLEEMVPWQGGGDMITKVCLPTGTTYAPLPLKFE
                     AGTANYIGIAGLGAAVEFINNLNSFDIEAHEKEITSNAMKALQEIEGCKIYGIPGTYY
                     GQVYEKAPVFSMSIEGVHPADIAILLDKLGIAARSGMLCAEPLLERYQLTAVLRASFA
                     LYNTQEELDFFVESLRKVLKMLR"
     gene            2399..2836
                     /locus_tag="PLM68_01215"
     CDS             2399..2836
                     /locus_tag="PLM68_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOT54025.1"
                     /translation="MTIQEKQKQIVEEFSFLEEWMDKYEYLIELGKKLPPLEDSLRLE
                     KNLIRGCQSRVWLAVSRENGKLVFKADSDALITKGIISLLVQVFSGHTPGEILESDFS
                     FIQKIGLKEHLSPTRANGLLSMIEQIRLYALAANTEKTAENGK"
     gene            2826..3155
                     /locus_tag="PLM68_01220"
     CDS             2826..3155
                     /locus_tag="PLM68_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOT54026.1"
                     /translation="MESELEKSIKRALRQIFDPEIPVNIVDLGLVYGIEIGKGDKEGH
                     PVTVIMTLTAPNCPLADQVVEEARFTISHVPGVSRATIKLTFDPPWDPSRMSEEALLE
                     TGLYGMF"
     gene            3168..3632
                     /gene="folK"
                     /locus_tag="PLM68_01225"
     CDS             3168..3632
                     /gene="folK"
                     /locus_tag="PLM68_01225"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOT54027.1"
                     /translation="MHTTYLLLGSNLGDRDSLMQQALEALYELGDITRISQRKETVPW
                     GFTETVPPFLNQAICLETRLEPEQLLDHCLEIEKKLGRERPVAESPGKDRKSYFSRLI
                     DIDILLYDNVRMNTGRLILPHPHIKDRDFARELLEEILPKSLSLKDFLPDDL"
     gene            3629..5170
                     /locus_tag="PLM68_01230"
     CDS             3629..5170
                     /locus_tag="PLM68_01230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT54028.1"
                     /translation="MTQEDLFKNIIAHAKEYGFIFPSSEIYDGLSAVYDYGQNGVELK
                     NNIRKYWWDSLVRMHDNIVGLDSAIFMHPKIWEASGHVAAFNDPLIDNKDSQKRYRAD
                     VLLEDYLAKLEEKVNKEVAKGKKRFGEQFDEVQFRATNPNVLREQAKIDSLQEKMIKA
                     LNGNDLVGLRQLILDCGIVCPVSGTRNWTEVRQFNLMFSTQLGSNGEGASQVFLRPET
                     AQGIFVNFLNVQKSGRMKVPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVKPG
                     EEMKWYEYWKDMRLRWHLSLGLGRDMYRFHNHDKLAHYANAACDIEFQFPFGFKELEG
                     IHSRTDYDLSRHEEFSGRKLRYFDPETNESYIPYVVETSIGLDRTFLAVLSASYREEE
                     LEDGETRVVLRLPHILAPVKLGIFPLVKKDGLPEVAQQILKTLRLEHACLYDEKDSIG
                     KRYRRMDAIGTPLCITVDHQTLEDNTVTIRDRDTMLQERVPVDNLPGILDEKLNSRNW
                     LGKLT"
     gene            5194..5580
                     /locus_tag="PLM68_01235"
     CDS             5194..5580
                     /locus_tag="PLM68_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOT54029.1"
                     /translation="MIFLLVVYLGGCTYFLFAKGDSLPAFKKAGMILWLASLLDVLPD
                     KIVHSLLFIPIIPLAWGAVRPRRIITKIVIFWSGLIFGVITEVIQHYLPYRTGDISDV
                     AADIVGSVLGIPFMLLFFSSEKKKRK"
     gene            5577..8495
                     /locus_tag="PLM68_01240"
     CDS             5577..8495
                     /locus_tag="PLM68_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54030.1"
                     /translation="MNEIILSGLLNLFALFGALAGIDKERAAKLIAAYLDQHFGVRRR
                     ETYLGLYQDLTELYELSPDLDKDKIIESVCEGLRKNIESSEQSLLLLRFMEFSAINRE
                     GFLKQEDLFHKVAAHFNVTGEELMHFKAFVLDGETDRVRSFRPEGWEGILRLIDLPGK
                     NVIAFTYRGNDQVMMNDVPVLQDTFQLWQQSAVMKGKHGNPLYYSNVQSLFQTGETAR
                     IPIELTGQGIEFRFPHSENGLHNFSFTLRSGQLVAIMGGSGVGKSTLLGLLNGNIKPQ
                     EGQILLNNHSIEDPFVQKLIGFVPQDDLLIEELTVFQNLWYTARLCFDGMSPAWLENK
                     VMEVLTDLGLAATRDLKVGSALNKTISGGQRKRLNIALELIREPAVLFLDEPTSGLSS
                     SDSEKVINLLKEQTFRGRLIVVNIHQPSSDIYKLFDRLWLLDKGGYPVFDGNPIEAVT
                     HFKQVAQHADAQTSMCATCGNVNPEIILNILESKSLDDTGRLTEKRRISPQEWHNKYL
                     ESRQEQEKPQIQDVPVTKQKKPSPWKQFLIFLERNLQTKKANTQYLLISLLEAPLLAA
                     IVAMLTRYAPETEYTVMDNKNLVSFFFMAVIVAIFMGMSVSAEEIFKDRSLLKRERFL
                     RLSHNGYIWSKIVYLAGLSLIQTLLFILVGHAIMGIHNMLAIWWIILFAASFVANLTG
                     LVLSQSLSSIVAIYITIPLLLIPQILLCGLVVKFDDLTPGSKTGNVPVIGEVIPSRWA
                     FEALAVSSYMYNPYMKNFFEEEKEKFRAQYYRLGFLEELQSQMETVQDEFLKSGNADQ
                     QRLRVIETGLPGLARITELEPFAHWTNTWSETLYQDLDSYFIQADKVLSRRSLMYTQA
                     IDKINKELLDELGKEGLLTLKKDNHNLFLQDLVLNTSVTNMYRLVDDVIVPKVGYIYL
                     EPSTRNGRAPFYSHVKILGSLKISTLWYNLAVLGLMAVLTSLALFFEIPARFMRKKST
                     "
     gene            8563..9387
                     /locus_tag="PLM68_01245"
     CDS             8563..9387
                     /locus_tag="PLM68_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54031.1"
                     /translation="MRKIAIALLFASALIVGACGNTGSKKKDAGTKSEVSLQEQYLTQ
                     DLLIKIDSLIAGIQKLGVMPHMKAIDEGTLVLTDREKRIKPTFLMPLEKVNDLVTLNQ
                     KSRALAVYAVDREIAQLYEMPLEEYDRVIAQLLVDTDNVSLMEGAEVKVEVNTMDVDS
                     WIVTMGEDQLAKEQMHLFFETLGAGVLESLYITSLDIPRYITYFNDQTASEVTYRLLL
                     VVDGIESLIPYHPELESIRGILEPLKVINAINVKQLEEQLNTLAVDIAEGRNKLLD"
     gene            9464..10420
                     /locus_tag="PLM68_01250"
     CDS             9464..10420
                     /locus_tag="PLM68_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54032.1"
                     /translation="MILKIALFLSMIIQLATAIVAVSMVRRTRYNVSWILISIAFVLM
                     ALERLVEFSSFFWKTKIIAKEEINSWLGILISILMLISLYYIRQIFNLQDQLDAMRKT
                     SEKRMLMGIIQGEENARRTIAGDLHDGMGPLLSSAKMIISAADTKKMNSENRKIVEQT
                     CTVIDEAIITLREISNHLSPHLLKNFGLVKAIETLTKQLFANSPIKVVLHSGISSKRY
                     DYDVEINVYRIVTELFNNSMKHAGAETIELGLKEELNHRLMVDYRDDGKGIEPGYFFR
                     QDPSSSGMGLDNIYSRVTSLDGSVSVESSPGKGFYAHIEIPV"
     gene            10420..11067
                     /locus_tag="PLM68_01255"
     CDS             10420..11067
                     /locus_tag="PLM68_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT54033.1"
                     /translation="MKKINIFLVDDHALFREGLHFLLLKMDFVDQILEAENGLDFLEK
                     IVGVNNCIVLMDIEMPLMNGIEATRKALERYPDLKILALSMYSEESYLSSMIEAGASG
                     FLLKNSSFSEVKSALRDVMEGKNYFSPAIIQSILEIMNQKINHPDKDLDEITQREKEI
                     LYYICKGFSNGEIAQQLGISKRTVDKHRENLLQKTQSRNTADLVTYAIKHHYFDI"
     gene            11165..11917
                     /locus_tag="PLM68_01260"
     CDS             11165..11917
                     /locus_tag="PLM68_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54034.1"
                     /translation="MIKSITIMPGKNKDGNPEYFSQIDIYPGEIVGVVGPTGSGKSAF
                     IEDIEQLAQADTATHRRVLINGSVPDISMRFDPKKKMIAQLSQNMNFLADMTVRDFLM
                     LHAKCRGKNPDMVEQVINHANTLTGEPITENMNLTVLSGGQSRSLMVADVAFISDSPI
                     VLIDEIENAGIKKHQAMKLLSGKGKIILVVTHDPLLALLTGRRIVMKNGGVEKIFNTT
                     THETDLCVELNRMDRELFRIRERVRLGEEIFA"
     gene            11928..12629
                     /locus_tag="PLM68_01265"
     CDS             11928..12629
                     /locus_tag="PLM68_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT54035.1"
                     /translation="MKVIVVAGTPGAGKTSVLLNTIKFLKEQQVTIAVAKIDCLYTED
                     HIRFKKTGIPTLVGLSRDMCPDHYTIYNMGEMVSFASEHTADMLIVETAGLCHRCAPY
                     TTNGLGVCVIDATTGPNTPLKVGPFLSTANVVAITKGDMISQAEREIFRERVMEMNPG
                     CRIIETNGLTGKGASELAGCFKDAPVFNYNENRLRHNAPFSICTLCVGEMHIEKKYHR
                     GVLRHINGVQEYKGE"
     gene            12633..13286
                     /locus_tag="PLM68_01270"
     CDS             12633..13286
                     /locus_tag="PLM68_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT54036.1"
                     /translation="MIKEETLLMLLPGFNCGACGYVNCATFASALKEKRATPEQCTVF
                     RQERFKEQFTKLTGMLNLAGIQVTETYTGLIDKAKADFLLHPLPGEPSCRETLACFSA
                     KKISKDDVIRYRPLGCPITHFARVVELDNGLLDVWVTGPGKLLGREEMVIDVGICMVL
                     SFRGVIEGMRPYVGQTVKFLPAHCMMGKVHSGVIVSLEDQTTRIDCIDLKIWEHTKV"
     gene            13332..15584
                     /locus_tag="PLM68_01275"
     CDS             13332..15584
                     /locus_tag="PLM68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT54037.1"
                     /translation="MKNTCKAALPILLLVFAATGCHRAEPVITSLSMLEKGKTFAVPA
                     GTAADQMVLDRIPDARIIYFNNVLHCALAVKEGKAHATAYDLPVLSNLQAKIEGLTVL
                     DEFIMDDQYGFAVRKEDNRLKEAMDRVLSKIQEDGTYARMLDRWFPKQGDPGPMPQIP
                     SSGENGILNFGTAAVTEPMTYVYANEGVIGFDIEYAAYIAQDLGMQLNIVDMEFGAML
                     PALISGKVDMIGAGLSITEERAKSVLFSEPYYKGGLAVMVREKPQQAKQAKRAKTLGV
                     LLGSTHEQYALTHYPNDRILQFQNVPDLLVSLRQGKSDAAMYVREMLAPVLKDNPDLG
                     ILADTIFYEPIGAVFNKENDSLRLQFNAFIQQIRGNGIYDQMVERWVTKQGEQMPEIE
                     KHMENGELRVAMVTSMGLPFIGVSQGSLVGYDVEIMMRFASYLGKKFVPIDMPFNSLL
                     LAVAMGKADMAVSCITITEERAKTVAFSDKYYECSVSIIADMSRMTRDSAEEFMPSET
                     TRSEKKGFFKKISESFHNNLVQEDRYKLVLDGLAVTVIISILAAILGTLLAGVVCSMR
                     MSASRFAATFAKGYISFFRGTPVLVLLMIIFYVVFASVQINPVLVAVIAFGLNFAAYV
                     AEIFRTSINSIGKGQKEAGIAAGFTRTQTMTHIILPQALRHALPVYKGEFISMVKATS
                     VVGYIAVQDVTRVSDIIRSRTFDAFFPLLVATVIYLALSWLLTWLLDKVEISVDPKRK
                     RVRKMKEETV"
     gene            15581..16654
                     /locus_tag="PLM68_01280"
     CDS             15581..16654
                     /locus_tag="PLM68_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT54038.1"
                     /translation="MIQVEHLSKKYGDLEVLKDVSVHIKKGEIISVIGPSGTGKSTFL
                     RCLNLLETPSGGKIVIDGESLLDKGTDVPRIRRKMGMVFQSFNLYSHLTVLDNLTIGP
                     VTLLKKKQAEADRKAMELLKMVGLAEKAYNFPDELSGGQQQRIAIARCLAMDPGILLF
                     DEPTSALDPTMVSEVLAVIRRLARDGMTMIIVTHEMEFARNISTRVFYMDEGVIYEEG
                     TPEQIFDHPRREKTRAFIQRVRSLQYAINSPLYDLYRLQGEIVLFCEKHVISPKISNR
                     LQIIAEEILQLQSDFTDILLQLSYLEKEDSLLMQCTAAGTPVNPLEQEDNEIRVTMLK
                     ALCESVEYSYNEGKNLLLLRVRR"
     gene            complement(17299..17372)
                     /locus_tag="PLM68_01285"
     tRNA            complement(17299..17372)
                     /locus_tag="PLM68_01285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17336..17338),aa:Pro,seq:tgg)
     gene            17569..18753
                     /locus_tag="PLM68_01290"
     CDS             17569..18753
                     /locus_tag="PLM68_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT54039.1"
                     /translation="MKDIILHLITTMLVFIYPRLASLNLELNSVSIESDSLMIGYGAA
                     YMLNIQVDPESSYNMVDLTWKSSDGQIVAVNEDGFLSAVGHGSATITVAASTADTTCT
                     DSCYVLVYPVPVRIPDTGFRSYCLSFADINADFMLTSDELAVVERISISDRGVKSLEG
                     LKYFTGLETLVCYNNSLTSLDVSGNPALKMILCANNEIATLDLSHNSSLEYIDCGNNK
                     LEKLNLEGNSNLALVICASNELANLDVSHNRLLEYLDCSKNHLKILDMTGNRALTVLN
                     CSDNELEELDLRNNSKLLMLGCGFNRLTALDVSANLLLENLNCLSNMLASLDVSRNTA
                     LLRLNCANNQLVELDLSAHKALQCQYLDCRNNPWLFELWLRSKQDISLYSMDEHTEVR
                     YR"
     gene            complement(19001..19090)
                     /locus_tag="PLM68_01295"
     tRNA            complement(19001..19090)
                     /locus_tag="PLM68_01295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19054..19056),aa:Ser,seq:gct)
     gene            complement(19205..20161)
                     /gene="floA"
                     /locus_tag="PLM68_01300"
     CDS             complement(19205..20161)
                     /gene="floA"
                     /locus_tag="PLM68_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137107.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOT54040.1"
                     /translation="MSSVGITLMWIAIGIVGLFILLWFFPITLWFQALISGVRVSLIQ
                     LVLMRWRKVPPSTIVMAMITGTKAGLKMDVNAMEAHYLAKGNIIKVVNALISADKANI
                     PLDFKMAAAIDLAGRDVFEAVQMSVNPKVINTPPVSAVAKDGIQLIAKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVSSIGSASSHKSVLENPDYISKVVLEKGLDSGTAFEILS
                     IDIADIDVGKNIGAVLQMDQANADKNIAQARAEERRAMAVALEQEKIADAQEARAKVI
                     LAEAEIPLAIAEAFRKGNLGIMDYYRLKNIQADTNMRDQIAK"
     gene            complement(20173..20643)
                     /locus_tag="PLM68_01305"
     CDS             complement(20173..20643)
                     /locus_tag="PLM68_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT54041.1"
                     /translation="MGLIITLIILGLLLIIAEMTIIPGFGITGILGLASFIGAIVMAF
                     TQFGSTVGFIVLFASLILCGILLFFVLRSKTWRKITLKQNIDHKAIPSPEEKGITVGM
                     EGISLTRLNPAGNGRFGDVTIEVRSSQGLIQAKTPIKVVYIEGLSIFVSPVENN"
     gene            complement(20655..22595)
                     /locus_tag="PLM68_01310"
     CDS             complement(20655..22595)
                     /locus_tag="PLM68_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54042.1"
                     /translation="MANKLHIALFLIAGLVLTASLAYAQQITAQDFFREGEKLQMDYQ
                     FERAKEAYTRAISLSGDSAFIARAEQRRVLCENGLILSNYVVSPHILGRAVIPYREFF
                     LYYDLSPSGAWALPPFELLRHLNDNGADRLPLFYRATLDRIVFTAKDSTSGSGYDLYM
                     IQKKDILANGASLWGPPVRLGPGINTPGNEIYPVLSSDGKTLYFSSDGLPGLGGMNLY
                     VSRWNTQTQEWGLAENLGIPFSSTHNDMLYMLSDDSRYFYFSSDRVAPKDSLVLYKVE
                     YESAPVKIRPSSVRELKEIASLPLRGDNTASVSNLQPTDTGPAIPDSVLQKTREYTAH
                     YAELTRTVRNLKNRVKEHENVLDSLRKRYGSLSIEEERALLANTIREEEFVLIELQQS
                     LRDARKSTQAIEDMFLSHGVLPPIAPAASESPVATAADQEHMSWYPVKQALASLEAHV
                     FETPVPPVPPVNLEFRIEKESVMVPWENEPPGLYYRIQLFTVSRKAPVSQLKGISPVF
                     EVRVGKQYVYYAGQFLRYADASRALTTTKRQGITGAIVVAYFQGKPIPVQEGRRREAQ
                     NKTAPEGIASYRVYLGSNEIPASLVSLVNELSDKDIIRVVTGTGADFFIGPFASVSQA
                     EDLARTLREKGFDMVSVQEVTN"
BASE COUNT         6065 a         5210 c         5768 g         5653 t
ORIGIN      
        1 tacgattacc tttttttcat tcgctcggac gtcttttacg tcatggttgt ttcataatat
       61 aatcaaaggc tttacgagat caaatccccg taagcctgag cgtccagcag atcggagatt
      121 tccccggggt ttatcaacct gatcttgacc atccagcctt taccataagg atcctcgtta
      181 actgtctgtg gcgcatcctc cagggattcg ttcacttctt ctatggttcc cgaaacaggc
      241 atgaaagcat ctgcaacggt tttaacggct tcgatggcgc cgaaaacctc acctctttcc
      301 agggtttcgc ccaaggtctg tatgtcaaca aaaacaatgt cgcccagctg ggattgtgca
      361 aaatctgtaa tgcccacaat ggccaggtcg ccttcaactt ttaaccactc atgctctttg
      421 gaaaacttga gattggctgg aatattcata acgattaagt ttaagtttag agaagcaaag
      481 atagctaaat atgccaaatt acaagagctt gagggcttgc cggatcagat cttccaggcc
      541 gaaggaagga ttttcctttt gcagacggga gatgaccttt tcaacggcag atcgctggaa
      601 gcccagttgc tgcagcgcag cagaagcctc gacaccagtt gccgatacag gcggagcctg
      661 ggtgccctgg gcgtcggggt ccagcccgat tttcatcatt ttatccctca ggtcaatgat
      721 catcctttcg gctgtctttg tgccgattcc cttgatgctt tttatccgga cgacatcacc
      781 cgtgacaatg gcattacgga gttctgcatt gctcatggcc gagaggacca tgcgtgcagt
      841 attgggcccg accccggtaa cccctatcaa caaccggaag atctcacgtt catcgcgtgt
      901 ggcaaaaccg taaagcagat gggcgtcatc ccttacctgg agatgtatcc acagcaagac
      961 atctttccgt tcatgaatgg ccgagtatgt ttgcagggat atgagcaaat gatagccgat
     1021 cccgcctgtt tccacaacgg cctgggcggg gtcggcagcg ataagggttc ccttgatgta
     1081 ttcgtacatt tttacagtgt ttactatact gcaaatatac atttattatc tttgaagcat
     1141 gaatctgtca caaatcaggg aacagtttcc cgccctgtca caaaaagtat acgggaaacc
     1201 tctcgtttac ctggacaatg gggctacatc ccagaaaccc aggcaggtac tggacgtcct
     1261 ccaggaaatg caagggggat tgaacgggaa catccaccgg gcagtccatt accttagcgt
     1321 gcagtccacc gaacggtatg acagggcacg tgccactata cgggatttca tcaatgccgc
     1381 ctctgtcaac gaggtgattt tcaccagcgg cactaccggc tcgctcaacc tgctggcttt
     1441 ttcgttcgga caaacctata tccgccctgg agaccgtatt gtgatcaccc gcgccgaaca
     1501 ccattccaat attgttccct ggcagatgat gtgcaaacgt accggggcca gtcttgaagt
     1561 atgggaggtt gaggagtcgg gagcaatgga tcccaaaaaa ctggaacaaa ttcttgacaa
     1621 acagggaagg aagcctaaac tgcttgccat agcacacata tccaatgttc tgggactggt
     1681 gaacccgctc agggaaatca ttcgtatagc ccattattac ggagttcctg tcgtcgtgga
     1741 cggggcacag ggtattgtcc ataaaaaagt cgacgtacag gatatggatg ccgatttcta
     1801 cgttttttca ggacacaagc tatacgggcc cacaggaacg ggcgtactgt acggaaagga
     1861 aaaatacctg gaagaaatgg ttccctggca gggcggggga gatatgatca caaaggtttg
     1921 cctgcccaca ggaaccacct atgccccgct tcccctcaaa tttgaagccg gtacggctaa
     1981 ttatataggg attgcaggcc tgggtgcagc cgtagagttt atcaacaacc tgaattcctt
     2041 tgacattgag gcacatgaaa aagagataac ctctaatgcc atgaaggccc tgcaggagat
     2101 agaaggttgc aagatatacg gcattcccgg aacctattac ggacaggtat atgaaaaagc
     2161 ccccgtcttt tccatgtcca tagaaggagt tcatcctgcc gatatagcca tcctgcttga
     2221 taaactgggc atcgccgccc gatctggcat gttgtgtgcc gaacccttgc tggagcgtta
     2281 ccagttgacg gctgtccttc gggcatcatt cgccctgtac aacacccagg aagaactgga
     2341 cttctttgtc gaatcactca gaaaggtgtt gaaaatgctc cgttaatccc gaattgtcat
     2401 gaccattcaa gagaaacaga aacaaatagt ggaggaattc tcttttctgg aggagtggat
     2461 ggacaaatac gagtacctca ttgaactggg taaaaaattg ccaccgctgg aagacagtct
     2521 gcgtcttgaa aaaaacctga taaggggatg ccagtccagg gtttggcttg ccgtaagcag
     2581 ggaaaacgga aaacttgttt tcaaggccga cagtgacgcc ctgatcacca aaggtatcat
     2641 ttctttgcta gtgcaggtgt tttcgggcca cacacccgga gaaatcctgg aatcagactt
     2701 ttctttcata cagaagatcg gtctgaagga acacctctct ccaacgcggg ccaacggact
     2761 gctgtccatg atagaacaga taagactata tgccttggct gccaatacag aaaagaccgc
     2821 ggaaaatgga aagtgaactt gaaaaaagca taaaacgtgc cctcagacaa atttttgacc
     2881 cggaaatccc ggtcaacata gtggaccttg gccttgtcta cggcattgaa atagggaagg
     2941 gggacaaaga gggacatccc gtgaccgtta tcatgaccct tacggcgccc aattgccctt
     3001 tggctgacca ggtggtggaa gaagcccggt ttacaataag ccatgtgccg ggagtttcga
     3061 gggcaaccat aaagctcaca tttgacccgc cctgggatcc ttcccgcatg agcgaagaag
     3121 cgctgctgga aacaggtctg tacggaatgt tttaatattt catgcccatg catacgacat
     3181 atcttctgct ggggagcaat ctgggagacc gtgacagcct tatgcaacag gcattggaag
     3241 cattatatga gctgggagac atcaccagga tttcccaaag aaaagaaacc gtaccatggg
     3301 gttttaccga aaccgttccg cctttcctga accaggccat atgtctggaa acacggctgg
     3361 aaccggaaca actgttggac cactgcctgg aaatagaaaa gaaactggga agggaaagac
     3421 ctgttgccga atctcctggc aaggacagga aatcatattt ttcccgtctg atagacattg
     3481 atatccttct gtatgataat gtgagaatga ataccggaag gcttatcctg ccacatcctc
     3541 atataaagga ccgggatttc gccagggagc tgctggaaga gattcttcca aaaagtctat
     3601 ctttgaagga ttttttacca gacgatttat gacacaggaa gatttattca aaaacatcat
     3661 tgcccacgca aaggaatacg gtttcatatt tccctccagc gagatttacg acgggttgag
     3721 tgccgtgtac gattacgggc agaacggggt agagctgaag aacaacataa ggaaatactg
     3781 gtgggattcg ctggtcagga tgcacgacaa cattgtaggg ctggactctg cgatcttcat
     3841 gcatccgaag atctgggaag ccagcgggca tgtggccgca ttcaacgatc cgctgataga
     3901 caataaggat tcccaaaaaa gataccgcgc cgatgtattg ctggaagatt acctggccaa
     3961 actggaggaa aaggtaaaca aagaagtggc caaaggtaag aaaaggttcg gggagcaatt
     4021 tgatgaagtt caattccgcg ccaccaatcc caacgtgctc agggaacagg ccaagattga
     4081 cagtttgcag gaaaaaatga tcaaggccct gaacgggaat gatctggtag gattgcgaca
     4141 gcttatcctg gattgtggga tcgtctgtcc ggtttcggga accaggaact ggaccgaggt
     4201 gcgccagttt aacctgatgt tcagcaccca gttgggaagc aacggcgaag gggcatccca
     4261 ggtctttctc aggcccgaga cggcccaggg aatctttgtg aactttctga atgtgcagaa
     4321 aagcggccgt atgaaagttc cctttggcat agcgcaaacc gggaaggctt tcaggaacga
     4381 aatcgtggcc aggcaattca ttttcagaat gcgcgaattt gaacagatgg aaatgcagtt
     4441 ctttgtcaag ccgggagaag agatgaagtg gtatgagtac tggaaggaca tgcgcctgcg
     4501 ctggcatttg tcactgggcc tgggaaggga tatgtaccgg ttccacaatc acgacaaact
     4561 ggcccactat gccaatgccg cctgtgacat tgaatttcag tttccttttg gtttcaagga
     4621 actggaaggg atccactccc ggacagatta tgatctctcg cgccacgaag aattctcggg
     4681 ccgtaaattg cggtattttg acccggaaac aaatgaaagc tatatcccct atgtggtaga
     4741 aacttctatt ggcctggacc gtacgttcct ggctgtattg tctgcctcgt accgggaaga
     4801 ggaacttgaa gacggtgaaa cacgggtggt gttgcgttta ccccacatac tggcccctgt
     4861 taagctgggg atatttcccc tggtcaagaa ggacggatta cccgaggtgg cgcagcagat
     4921 actcaaaacg ctccgcctgg aacatgcctg cctgtatgac gagaaggaca gcataggaaa
     4981 acgctatcgc aggatggatg ccattggcac gcccctttgc ataacagtgg accatcagac
     5041 cctggaagac aataccgtaa ccatcaggga cagggatacc atgctccagg aaagggttcc
     5101 ggttgacaac ctgcccggaa tactggatga aaaattaaac agcagaaatt ggctcggaaa
     5161 actgacataa aaaaaggatt tggaaggatt ttcatgattt ttctcctggt ggtttacctg
     5221 ggaggatgca cctatttttt atttgcaaag ggagattccc tgccagcatt caagaaggcc
     5281 gggatgatct tatggctggc ttccctgctg gatgtgttgc ccgataagat cgtccattcg
     5341 ttgctgttta ttcccattat tccccttgcc tggggagccg taaggccccg caggattatc
     5401 acaaagattg tcatattctg gtccggactg atttttgggg tgataacaga agtaattcag
     5461 cactacctcc cgtacaggac cggggatatt tcggatgtgg cagcagatat tgtaggaagt
     5521 gttctgggca ttccctttat gctgctattt ttttcaagtg aaaaaaagaa acgaaaatga
     5581 atgagattat tttatcggga ctgctcaatc tctttgcctt attcggggct ttggccggga
     5641 tagacaaaga gcgggcagca aaactcattg cagcctatct tgaccagcac tttggcgtac
     5701 gccgcaggga aacttacctg ggtctgtacc aggatcttac agagctttat gagttgtctc
     5761 cggatctgga caaggacaag atcatagaat ctgtatgcga aggattgcgc aaaaacattg
     5821 aatcatccga acagtctttg ctcctgctgc gtttcatgga gttttccgcc ataaacaggg
     5881 agggcttttt aaagcaggag gatctttttc ataaagtagc cgctcatttt aatgtaaccg
     5941 gtgaagagct catgcatttc aaggcttttg tactggacgg agagactgac cgggtgcgca
     6001 gtttcaggcc ggaaggatgg gaagggatat tgcgactgat agaccttccg gggaagaatg
     6061 tgattgcttt tacttaccgg ggaaatgacc aggtgatgat gaacgatgtc ccggtattgc
     6121 aggacacgtt ccagctgtgg cagcaaagtg cagtcatgaa gggaaaacac gggaatccac
     6181 tgtattacag caacgtacaa tctctttttc aaacagggga gaccgccagg atccctattg
     6241 aactaacagg tcagggtatt gaattccgct ttccacacag cgaaaacggg ctgcacaatt
     6301 tctcttttac gctcagatcg gggcagctgg tagctatcat gggaggaagc ggggtgggga
     6361 aatccacatt gctgggcctg ctcaacggaa acatcaaacc gcaggaagga cagatcctgc
     6421 ttaataacca ctccatagaa gacccgtttg tgcaaaagct catcggcttt gtgccccagg
     6481 atgatctgct gattgaagaa ctgacggtgt ttcagaacct ctggtatacc gccaggcttt
     6541 gttttgacgg gatgtcccct gcatggctgg aaaataaagt gatggaagtg ctgacagacc
     6601 tgggtcttgc cgctaccagg gacctaaaag tgggctcggc gctgaacaag acaatcagcg
     6661 ggggacaacg caaaaggctc aacatagcgc tggagctgat cagggagccg gcggtgctgt
     6721 tcctggacga acccacctcg ggcctgagct cttcggactc ggaaaaggtt attaatttac
     6781 tgaaagaaca aacgttcagg ggccggctca ttgtggtcaa catccaccag ccgtcctcgg
     6841 atatttataa attgtttgac cgcctgtggc tgctggacaa gggagggtac ccggtttttg
     6901 acgggaatcc tatagaggcg gttacgcatt tcaagcaggt ggctcaacac gccgacgcgc
     6961 aaacgagcat gtgtgccaca tgcgggaatg taaatcccga gattattctc aacatactgg
     7021 aatccaaatc tttggatgat accgggcgcc ttaccgagaa acgccgcata agcccccagg
     7081 aatggcacaa caaatacctg gaatcaaggc aggagcagga aaaaccacag atacaggatg
     7141 tgcctgtgac aaaacagaaa aaaccctccc cgtggaaaca attcctgata ttcctggaac
     7201 gtaatctgca gacaaagaaa gccaatacgc agtatttgct catttcactg ctggaagctc
     7261 ctttgctggc agccattgtg gccatgctca cacggtatgc cccagagacg gagtacaccg
     7321 tcatggacaa caagaacctg gtctcgttct ttttcatggc cgtcatagta gccatattca
     7381 tgggcatgag cgtgagcgca gaggaaatct ttaaagaccg ttccctgctc aaacgggaac
     7441 gttttttacg cctgagccac aacggataca tctggtcaaa aattgtctac ctggccggtc
     7501 tttcactcat ccagacactg ttgttcatct tggtggggca tgccatcatg gggatacaca
     7561 acatgttggc tatctggtgg atcattctgt ttgcagcttc ctttgtggca aatctcacag
     7621 gactggtgct ttcacagtcc ttaagctcca ttgttgccat ctatatcacc attccactgc
     7681 tgctcattcc ccagatcctg ttgtgcggtc tggtggtcaa gtttgatgac ctgacaccgg
     7741 gcagcaagac agggaacgtg ccggtgatag gcgaggttat cccttcgcgc tgggccttcg
     7801 aagccctggc agtgagctct tatatgtaca atccgtacat gaagaatttt tttgaggagg
     7861 aaaaggaaaa attcagggcg caatattaca ggcttggatt cctggaagaa ctgcagagcc
     7921 agatggaaac agtacaggat gaattcctga agtccgggaa tgcagaccag caaaggcttc
     7981 gtgtcatcga aaccggattg cccggacttg caaggataac agagcttgag ccatttgcgc
     8041 attggacaaa cacctggtcc gaaacgcttt accaggacct ggattcctat ttcatacagg
     8101 ccgataaggt cctctccaga aggtctttaa tgtataccca ggcgattgac aagataaaca
     8161 aggaacttct ggatgagctg ggcaaggaag gattgctaac cttgaaaaaa gacaaccaca
     8221 acctgttcct gcaggacctg gttctcaata catcagtaac aaacatgtac cggctggtgg
     8281 atgatgtgat cgttcccaag gtcggttaca tatacctgga accatcaacc cgcaacggcc
     8341 gtgctccctt ttacagccac gtgaaaatcc tgggatcatt gaaaatttcc accctctggt
     8401 ataacctggc cgttttggga ttgatggccg tgctgacctc gctggccttg ttttttgaga
     8461 ttcccgcgag gtttatgaga aagaaaagca cataataaag ggtttttttt atatttgtat
     8521 gattattccg gaaacattat aaacattcaa aaaagcataa acatgagaaa aatcgccatt
     8581 gctctgttgt tcgcctctgc actgatagtg ggtgcctgcg gcaacactgg ctccaaaaag
     8641 aaagatgccg gcacaaaatc agaagtttcc ctgcaggagc aataccttac acaggattta
     8701 ttaatcaaga tcgactcact gatagcagga atccagaaac tgggtgtcat gccccatatg
     8761 aaagccatag atgaagggac cctggttctt accgatcgtg agaaaaggat aaaacccact
     8821 tttctgatgc ctctggaaaa agtgaacgac ctggtcacac tgaaccagaa aagcagggca
     8881 ctggccgttt atgcagttga cagggaaatc gcccagctgt acgaaatgcc tcttgaagag
     8941 tacgacaggg tgatagccca gctcctggtg gataccgaca atgtttcgct gatggaagga
     9001 gccgaggtca aggtggaagt gaacaccatg gacgtggatt cctggatagt gaccatgggt
     9061 gaagaccaat tggcaaagga acaaatgcat ttattttttg aaacactggg ggcaggcgtt
     9121 ctggaaagcc tgtacataac cagcctggat atcccgcgtt atatcacata cttcaacgat
     9181 caaacagcct ccgaagtcac ctacaggcta ttgctggtgg tagacggcat agaatcactg
     9241 ataccctacc atcctgaact ggagtccatt cgcgggatac tggaaccgtt gaaagtaatc
     9301 aatgccatta acgtaaaaca actggaagaa caactgaaca cactggcagt agatattgct
     9361 gaaggacgca ataagctgct tgattgatac ttaaaaccta atcgtgtgta agccgcttct
     9421 ttaatgggcg gcttttttat tttatactaa atttacaaga aaaatgattt taaaaattgc
     9481 attattcctg tctatgatca ttcagctggc aacagccatt gtggccgtca gcatggtacg
     9541 cagaaccagg tacaatgtgt cgtggattct gatctcaata gcctttgtcc tgatggcgct
     9601 cgaacggctg gttgagtttt catcattttt ctggaagacc aaaattatcg ccaaagaaga
     9661 gattaacagc tggctgggta tcctgatttc catcctgatg ctcataagcc tctattatat
     9721 caggcaaatt ttcaacctac aggaccagtt ggatgctatg cgcaagacga gtgaaaaacg
     9781 catgttgatg ggaattatcc agggcgaaga gaatgcccgt cgtaccatag ccggagactt
     9841 gcacgatgga atgggcccgt tgctttcttc ggcaaaaatg atcataagtg ccgccgatac
     9901 caaaaaaatg aattcggaaa accggaagat tgtagaacag acgtgtacag tgattgacga
     9961 ggcaataatt acgcttcggg aaatatcaaa tcacctgagt ccgcatctgc tgaaaaattt
    10021 tggtttagtt aaggcaatcg aaacccttac aaaacaactg tttgccaatt ctcccataaa
    10081 agtggtttta cactccggca tttcttctaa aaggtacgat tacgatgtag agatcaacgt
    10141 ttaccggatt gtgactgaat tatttaacaa cagtatgaaa cacgccgggg cggaaaccat
    10201 agaactgggg ttaaaggaag agctgaacca ccggctgatg gtagattacc gggacgatgg
    10261 gaaaggaata gaacccggat atttttttag gcaggatcct tcttcttccg ggatgggtct
    10321 ggataacatc tattcgcgcg tcacgtcact ggatggttct gtttcggtgg aatcgtcccc
    10381 ggggaaagga ttttatgctc atattgaaat acctgtgtga tgaaaaaaat caatatattt
    10441 ttagtggatg accatgcctt gtttcgggaa ggcctgcatt ttttgcttct taaaatggat
    10501 tttgtagacc agatactgga agcggaaaac ggcctcgatt ttctggaaaa aatagttggg
    10561 gtaaacaact gtattgtatt aatggatata gagatgcctt taatgaacgg aatagaagct
    10621 acaagaaaag cacttgaacg ctatccggat ttaaaaatac ttgccttgtc catgtattca
    10681 gaggaaagtt acctttccag catgatagag gcgggggcgt cgggtttttt attgaaaaat
    10741 tcttccttta gtgaggtgaa aagtgctttg cgggatgtta tggaagggaa gaattatttt
    10801 tctccggcca taatacaatc cattctggaa attatgaacc aaaagatcaa ccatcctgat
    10861 aaggatctgg atgagataac ccaacgtgaa aaggaaattc tgtattatat atgcaaaggg
    10921 ttctccaatg gcgaaattgc acaacaactg ggcataagta agcgtacggt agacaaacac
    10981 cgggaaaacc tactccagaa aacacagtca agaaacacgg ccgacctggt tacctatgct
    11041 atcaaacacc attattttga tatctgagat cacaatgata cgtgttttaa cgtagctgcg
    11101 gatacgtttt tcatgggttt ataaaaatag taaaagcttt tacctttgtc taaaataaac
    11161 ggcaatgata aaaagcatta ccataatgcc cggaaaaaac aaagacggga atcccgaata
    11221 cttttcacag attgatattt atccgggtga aatcgtcggg gttgtagggc ctaccggaag
    11281 cggcaaaagc gcttttattg aagatattga acagctggca caggccgata ctgccacaca
    11341 ccgtcgtgtg cttataaacg gaagcgttcc tgatatttcc atgcggtttg atcccaaaaa
    11401 gaaaatgatt gcccaattgt ctcaaaacat gaattttctg gcagatatga cggtacggga
    11461 ctttttgatg cttcacgcca aatgcagggg aaagaatccg gatatggtgg aacaggttat
    11521 taatcatgcc aacacattga ctggtgagcc gataaccgaa aacatgaatc tgacggtcct
    11581 gagcggagga caaagccgtt cactgatggt ggcagatgtt gcttttatca gtgactcacc
    11641 catagtcctg atagatgaaa ttgaaaacgc aggcattaag aaacaccagg ctatgaaatt
    11701 attgtcgggc aagggtaaga tcattttggt ggtgacccat gatccgttgc tggccttgct
    11761 gaccggccgg cgcattgtaa tgaaaaatgg cggggtagaa aagattttca ataccactac
    11821 tcatgagacg gatctttgcg tggaattaaa ccggatggac cgggaactgt tcaggatccg
    11881 cgaacgggtg aggctaggtg aagaaatctt tgcctgacaa gagaaccatg aaagtaattg
    11941 tagtagccgg tacgccggga gcaggcaaaa cgtccgtatt gctcaatacc attaaatttc
    12001 tgaaagaaca gcaggtaacg attgccgtgg ccaagattga ctgtttgtat accgaagacc
    12061 atatccgttt taaaaagaca ggaattccaa ccctggtggg tttatccagg gatatgtgcc
    12121 ccgatcatta caccatttac aacatgggag aaatggtatc ttttgccagt gagcataccg
    12181 ccgatatgct catagtagaa acagccggat tgtgtcacag gtgcgctccc tataccacca
    12241 atggccttgg ggtttgtgtg atagatgcca caacggggcc caataccccg ctaaaagtcg
    12301 gtcctttttt aagcacggcc aatgtagtgg ccataactaa aggcgatatg atctcacagg
    12361 cagaaaggga aatattcagg gaaagagtaa tggaaatgaa tcccggatgc cgtatcattg
    12421 aaacaaacgg attaaccgga aaaggggctt ccgaattggc gggatgtttt aaggatgccc
    12481 ccgtgtttaa ttacaatgag aaccgcttgc gccataatgc gccgttttcc atatgtacgt
    12541 tatgcgtagg ggaaatgcat attgaaaaga aataccatag gggagtactg cggcacatta
    12601 acggggttca ggaatataag ggagaataag ccatgatcaa ggaggaaacc ctgttgatgt
    12661 tgttgccggg attcaattgc ggtgcctgcg ggtatgtgaa ttgcgcgact ttcgcctcgg
    12721 ctttgaaaga aaaaagagcc acaccggaac aatgcactgt tttcaggcag gaacggttca
    12781 aggagcagtt cacaaaattg accgggatgc tgaatctggc cggcattcag gtgacagaaa
    12841 cctacacggg cctgattgat aaagcaaaag ccgatttcct gttacatccg ttacccggag
    12901 aaccctcctg ccgtgagaca ctggcctgtt tctcggccaa aaagatatcc aaagacgatg
    12961 tgatccgtta tcgtccgctg ggatgtccca ttacccattt tgcgcgggtt gtggagctgg
    13021 ataacggatt attggatgtg tgggtgacag ggccggggaa actgttggga agggaggaga
    13081 tggttattga tgttggcatt tgtatggtgc tttcattccg gggggtaatt gaagggatgc
    13141 ggccgtatgt ggggcaaaca gtgaaatttc tgccggctca ttgcatgatg ggaaaagtgc
    13201 attcaggagt gatcgtatct ttggaagacc agactacgcg aatagactgt atagacctca
    13261 aaatctggga acacacaaag gtttaagcgg aatcaccatt tttttgattc cggctttatt
    13321 atctttgttc catgaaaaac acgtgtaaag ccgccctgcc tatcctgttg cttgtgtttg
    13381 ccgcaacagg ctgtcacagg gcggaacctg ttattaccag cctttccatg ctggaaaaag
    13441 gaaaaacctt tgccgttcct gcgggaacag cggctgatca gatggtcctg gaccgcattc
    13501 ccgatgcccg gatcatttat ttcaacaacg tgctccattg tgctttggcc gtaaaagaag
    13561 ggaaagcaca cgccacggcg tatgacctgc cggtactgtc caatttacag gcaaaaattg
    13621 agggactgac cgttctggat gagttcatca tggacgacca gtacggcttt gcggtccgca
    13681 aagaggacaa caggctgaaa gaagccatgg accgggtatt gtcaaagatc caggaggacg
    13741 ggacttatgc ccggatgctt gaccgctggt tccccaaaca gggggatccc ggtccgatgc
    13801 cgcaaattcc ttcaagcgga gaaaacggta tcctgaactt cgggacggct gcagttaccg
    13861 aaccgatgac atatgtatat gcaaatgagg gagttattgg ttttgatatc gaatatgcgg
    13921 catacatagc ccaggacctg ggcatgcagc tcaatattgt tgatatggaa ttcggggcaa
    13981 tgctcccggc tcttatctcg ggaaaagtgg atatgatagg agccggattg tccataacag
    14041 aagaaagggc caagagcgtc ctgttttccg agccctacta taaaggtggg ctggccgtca
    14101 tggtccggga aaaacctcag caagcgaaac aggcaaaacg ggccaaaaca cttggtgtac
    14161 tattgggatc cacccatgag caatacgccc tgacccacta ccccaatgac cgcatactgc
    14221 aattccagaa tgtacccgac ctgctggtgt cgctacgtca gggaaaatca gatgcagcca
    14281 tgtatgtccg cgaaatgctg gctcccgtgt tgaaagataa tccggacctg ggcatattgg
    14341 ccgatactat tttttatgaa cccatagggg ctgttttcaa taaggaaaat gactccctgc
    14401 gtttgcagtt caatgctttt attcagcaga tccgggggaa cggtatttat gaccagatgg
    14461 tggaacgatg ggtgaccaaa caaggggaac agatgccgga gattgaaaaa cacatggaaa
    14521 acggggaatt gcgggttgcc atggtaacca gcatggggct tccttttatc ggcgtcagtc
    14581 aggggagcct ggtaggttat gatgtggaga tcatgatgcg ctttgcctca tacctgggga
    14641 aaaagttcgt cccaatagat atgcctttca atagtttgtt acttgccgtg gctatgggaa
    14701 aagcggacat ggcggtctcc tgtatcacga ttactgagga aagggcaaaa acagtggctt
    14761 tttctgataa gtattacgag tgcagtgtaa gcatcattgc cgatatgagc cgcatgaccc
    14821 gcgatagtgc cgaagaattc atgccgtcgg aaaccacccg gtcagaaaaa aagggttttt
    14881 tcaaaaaaat aagtgagagc tttcacaaca acctggttca ggaagaccgt tataagctgg
    14941 ttctggacgg cttggctgtg acggtcatca tttccatcct ggcagccatc ctgggcaccc
    15001 tcctggcagg ggtggtctgc tccatgcgca tgtcggcctc gcgttttgct gccacttttg
    15061 ccaagggcta catctctttc tttcgtggca cgccggtcct ggtcttgctg atgatcatct
    15121 tctatgtggt gtttgcctcg gtccagatca acccggtgct ggtggcggtc attgccttcg
    15181 gattgaattt tgccgcgtat gttgccgaga ttttccgcac gtccatcaac agcatcggca
    15241 aggggcaaaa agaggctggg atagctgccg gtttcacccg cacacagacc atgacccaca
    15301 tcatcctgcc acaggcgctg cggcacgctc tgccggtgta caagggtgaa ttcatttcca
    15361 tggtcaaggc cacctcggtc gtgggataca ttgccgtaca ggacgtcacc agggtgagcg
    15421 acatcatccg cagccgcacc ttcgatgcct ttttcccgtt gctggtagcc acggtcatat
    15481 acctggccct gtcctggctg ctgacctggt tgctggataa ggtagagatt tctgtggatc
    15541 ccaaaagaaa acgcgtgcgt aaaatgaagg aggaaacggt atgatacagg tagaacattt
    15601 gtccaagaaa tatggggacc tggaagttct gaaagatgtg agtgttcaca taaaaaaagg
    15661 cgaaatcatt tctgtaatcg ggccgtcggg aactggtaag agcaccttcc tgcgctgcct
    15721 gaatttactg gaaacgccta gcggaggaaa gattgtcatt gacggggaat ccttgctgga
    15781 taaaggcacg gatgtgccgc ggatccgccg gaaaatgggt atggtcttcc agtctttcaa
    15841 cctctattcc cacctgaccg tcctggataa cctgacgatc ggtcctgtga ctcttttgaa
    15901 aaagaagcag gccgaggctg accgcaaggc tatggagctg ctcaaaatgg tcggtctggc
    15961 agagaaagcc tacaattttc ccgatgaatt gtccggcggg cagcagcagc gcattgccat
    16021 tgcccgctgc ctggccatgg atccggggat cctgcttttt gacgagccca cctcggccct
    16081 ggatcctacc atggtaagtg aggtgctggc tgtgatccgg cgtctggccc gggatggcat
    16141 gaccatgatc atcgtcacgc acgaaatgga attcgcgcgc aatatttcca cccgtgtttt
    16201 ctatatggac gaaggggtga tctatgagga aggtaccccg gaacagatat ttgaccatcc
    16261 acgccgggaa aagaccaggg ctttcatcca gcgggtaagg agcctgcagt atgcgatcaa
    16321 cagcccgctg tatgacctgt accggctgca gggcgaaata gttctcttct gtgaaaagca
    16381 tgtgatctcc ccgaaaatat ccaatcggct acaaatcatt gctgaagaga tacttcagtt
    16441 acagtccgat ttcacagaca tactcctgca gctttcttat ttggaaaaag aagactctct
    16501 gctgatgcag tgtacggcag caggaacacc cgtaaatccg ttggaacagg aagacaacga
    16561 aatccgcgtc accatgctga aggcgctctg cgaaagcgtg gaatacagct ataacgaagg
    16621 caaaaacctg ctgctgctgc gggtcaggag gtaaaacaac tatttcaggc gccacttcct
    16681 ttcccctgac ccctaaattg ttaaaccggt attcacccat ggtctgcttt atcagaaatg
    16741 agcccaatcc ttccatatcc tgttcaagga tattcgcagc agggtcatac ccttgtatca
    16801 atgaaggatc atagggtaat ccaatatcgc gaatatgaat ctctatccca tcaagcctgc
    16861 ctttaaactc cacctggtaa tcggctattt catcagggtg aaaagatgtt ttcatcacat
    16921 taatacaggt ttcttcgacg gccagctgga ttttgtaaca gtcgcgtgac gaaaatccgg
    16981 ctattttggc aatttcagca gtgtatgatt acagggcagg cagcagactt tcttcgttag
    17041 gcatgctcag ctgacccaat ttttttttgc ttttattggg attttgaaat gatttaaata
    17101 aatatataac aatatttttt tccacaatgc tgtaggtgag gaagtaagat ggccgcagca
    17161 ctgtaaagaa cagcgcgctt ttttaatacg ccgaggcgcg ctgttcttta cagtgctgcg
    17221 actgcccatt agtgcagctg aagccttggc ataaacaaat aaaaaaaccg gttaaaattg
    17281 cttataaccg gtttcctgtc ggggtggtcc gactcgaacg gacgaccccc tgctcccaaa
    17341 gcaggaacgc taaccaactg cgctacaccc cgaaacttca gggctgcgaa gatagggaat
    17401 tttttttatt ttattccttt aagcgctttc tcgtattttt ttttccactt ctttccagtt
    17461 gatcacattc cggaagactt ccctgtaatc tgcccgttca ggaggtgtca aaaattatat
    17521 tatctttaaa tgatattttg tgtcgggttc tttctaaaaa ttaccacgat gaaagatatt
    17581 attcttcact taataacgac catgcttgtt ttcatatatc cccgtcttgc gtcgttaaat
    17641 cttgaactca atagtgtttc catagaaagt gacagtctga tgattggcta tggagctgca
    17701 tatatgctaa atatacaggt agatccggaa tcgtcttata acatggtgga tctgacctgg
    17761 aaaagcagtg atggacagat tgttgccgtg aatgaggatg gtttcctgtc ggccgtgggt
    17821 catggcagtg caaccattac ggtggcagcg tctacggcag acacaacctg taccgattct
    17881 tgttatgtgc ttgtgtatcc tgtcccggta aggatcccgg ataccggctt cagatcatat
    17941 tgtctgtctt ttgccgacat caatgcagat ttcatgctta cgtcagatga actggcagtt
    18001 gttgaaagga tcagcatttc tgacaggggg gtcaaatcac tggaaggact gaagtatttc
    18061 acgggcctcg aaaccctcgt ctgttacaat aattcgttga caagccttga tgtgagcgga
    18121 aatcctgcac taaaaatgat cctgtgtgcg aacaacgaga ttgcgacatt ggacctgagc
    18181 cataactcat ccctggagta catagattgc ggcaacaata aactggaaaa gctgaacctg
    18241 gaagggaaca gcaacttggc cctggtcata tgcgccagca atgaactggc aaacctggat
    18301 gtcagccata acaggttgct tgagtatctg gactgcagca aaaaccactt gaagatactg
    18361 gatatgacag gaaacagggc tttgaccgtt ctcaactgca gtgacaatga gctggaggaa
    18421 ttggatctca ggaacaatag taagctgctc atgctggggt gtggattcaa caggctcact
    18481 gcactggatg taagcgctaa tcttttgctg gaaaacctga attgcctgag caacatgctg
    18541 gcatcgctag atgtgtcgcg caacaccgct ctattgcgtc ttaactgtgc caataatcag
    18601 ttggttgaat tggacctgag tgctcacaag gcgttgcagt gccagtacct ggactgcagg
    18661 aataatccct ggctgtttga gttgtggctg aggagcaaac aggatatctc gctatattcc
    18721 atggatgagc atactgaggt gaggtacagg tagagggggg attacctcgc ttcgctccgt
    18781 gatccctatt aggggaggct tcaaaactta acagtttttc agcaatcaag gggattgcct
    18841 cccgttggtc ggcgttcccc ttgattggat cctcaaagaa aaaacaagcc aaactgtcgc
    18901 agacagttta aaagcaaaaa aaacagaccg tctttgagca agctcagacg gtccttattt
    18961 ttttgctttt tccctttggg ggtttggctt gttttttctt tggcggtgag ggggggattc
    19021 gaacccccgg tacccttgcg agtacgtcag tttagcaaac tgatggtttc agccactcac
    19081 ccacctcacc aaacctttac ttttaaggag ggcaaaggta tatattttat tttatttttc
    19141 aattagtccg caccttcatg caaggccggg aatcaggcag ccgggataca ggtgcaggaa
    19201 atttttattt ggcaatctgg tcgcgcatgt tggtgtcggc ctggatgttt ttcaggcggt
    19261 agtaatccat aatgccgagg ttgcctttgc ggaaagcttc tgcgatggca agcgggattt
    19321 ctgcctcggc aaggatgacc ttggcgcgtg cctcctgggc atcggctatt ttttcctgtt
    19381 ccagggcgac cgccatggcg cggcgttctt ctgcgcgggc ctgggctatg ttcttatcgg
    19441 cgttggcctg atccatctgc agcacggccc cgatgttttt acccacgtca atgtctgcaa
    19501 tgtcaatgga aaggatctcg aaggcagtcc cggaatcaag tcccttttcc agaaccactt
    19561 ttgaaatgta atccgggttt tccaataccg atttgtggga agaagcagag ccgatgcttg
    19621 aaacgatgcc ttcacccacg cgggccagga cggtttcttc tccagccccg ccaaccagtt
    19681 gcttgatgtt ggccctgacc gtgacccggg ctttggcgat aagctggatg ccgtcttttg
    19741 caacggcaga cacgggcggg gtgttgatca ctttgggatt gacagacatt tggaccgctt
    19801 cgaacacgtc acgtcctgcc aggtcaatgg cagcggccat tttgaagtcc agtggaatat
    19861 tcgctttatc ggcagagata agggcgttta ccaccttgat aatgtttccc ttggccaggt
    19921 aatgggcttc catggcattt acatccattt tcaaaccggc ttttgttccg gtgatcatgg
    19981 ccatgactat ggtgctgggc gggactttcc tccaacgcat gagcaccagt tggatcaggc
    20041 tgacacgcac accggatatc agggcctgga accaaagggt gatggggaaa aaccataaaa
    20101 ggatgaataa accaacgatc ccgatggcta tccacattaa cgttatacct acacttgaca
    20161 taatattttc ttttagttgt tttctacagg agacacaaat atactcaatc cctcgatata
    20221 gaccaccttt attggggtct tggcctggat caggccctgg gaagagcgta cttctatggt
    20281 tacatcacca aaccggccgt taccggcggg attcagtcgg gtaaggctta tcccttccat
    20341 tcccacggta atgccttttt cttcaggaga agggatggct ttatggtcga tgttctgctt
    20401 gagggtgatt ttacgccagg tctttgagcg caggacaaaa aagagcagga tgccgcacag
    20461 gatcaatgac gcgaacaaca caataaaacc gacagtgctt ccgaactggg tgaaggccat
    20521 cacaatggcg ccaatgaagc tggccagacc caggatgccg gtgatgccga agccggggat
    20581 aatggtcatc tcggcgatta ttagcaaaag gcccaagatg ataagtgtta tgatcaatcc
    20641 catatcttat gtttttaatt ggttacttcc tgaacagata ccatgtcaaa ccctttttca
    20701 cgaagggtcc tggccaggtc ttctgcctgt gaaacggatg cgaacggtcc tatgaagaaa
    20761 tcggccccgg tgccggtgac taccctgatg atatccttgt ctgagagctc attgaccagg
    20821 gagacaaggc tggcaggtat ctcattgctg cccaggtaaa cccggtacga ggcgatgcct
    20881 tccggagcgg ttttgttttg agcctccctg cgccgtcctt cctgaaccgg tatgggtttc
    20941 ccctggaaat aagccacgac tatggccccg gtgattccct gcctttttgt ggtcgtaagg
    21001 gctcgggaag catcggcata acggaggaat tgtccggcat agtaaacgta ttgctttccc
    21061 acacgcactt caaatacagg gctgatgcct ttcagctggc tgacaggggc tttcctggaa
    21121 accgtgaaca gctggatgcg gtaatacagg ccagggggct cgttttccca gggaaccatc
    21181 acggactctt tttcaatgcg gaattcaagg tttacaggcg gtaccggtgg cacaggggtt
    21241 tcaaacacat gggcttccag gctggctaat gcttgtttga caggatacca ggacatatgt
    21301 tcctgatcgg ctgcagttgc aacaggactt tcagaagcgg caggggcaat ggggggcagc
    21361 actccgtggg aaaggaacat gtcttctatg gcttgagtcg atttgcgggc atcgcgaagg
    21421 gattgctgca attctatgag aacgaattct tcttccctta ttgtgttggc caggagagcc
    21481 ctttcctctt caatagaaag agaaccatag cgttttcgta aactgtccaa tacattttca
    21541 tgttctttga cgcggttttt caggttcctg accgtccttg tcagttctgc gtagtgggct
    21601 gtatattcgc gtgttttctg caacaccgag tcaggaatgg caggcccggt gtctgttggc
    21661 tgcaggttgc tgaccgatgc cgtattatca cccctcaagg gcagggaagc gatttctttc
    21721 agttccctaa ccgaggaggg ccggattttc accggggcac tttcatattc caccttgtac
    21781 aacaccaggg aatcttttgg ggcgacacgg tccgaggaaa aataaaaata acgagaatca
    21841 tctgaaagca tgtacagcat gtcattgtgc gttgaagaga aggggatgcc caaattttct
    21901 gccaaccccc attcctgcgt ctgagtgttc cagcggctta cgtaaaggtt cattcctccc
    21961 aggccgggaa gtccgtcgga gctgaaatac agggttttac catcggaact aaggacaggg
    22021 taaatctcat tgcccggagt attgatcccg ggccccaggc ggacgggcgg accccataaa
    22081 gaagctccgt ttgccaggat atctttcttc tgtatcatat ataggtcata acctgagccg
    22141 gaagtggagt cctttgccgt aaagactata cggtccaggg tggctctgta aaaaaggggg
    22201 agccggtcag ccccgttgtc gtttagatgc ctgagcagct cgaatggggg cagggcccat
    22261 gctccggaag gtgacagatc gtagtacagg aaaaactccc ggtaagggat tacagcccgg
    22321 cccagtatat gtggactgac cacataatta ctcagaatca gcccattctc acataatacc
    22381 cggcgttgtt ccgcacgggc tatgaaagcc gaatcacccg acagactgat ggcccttgta
    22441 tacgcttctt tggcacgctc gaactggtag tccatctgga gtttttctcc ttcacgaaaa
    22501 aagtcctgcg cagttatctg ttgggcatat gctaacgaag cggttaaaac aagacccgct
    22561 atcaaaaaca gtgctatgtg cagtttattg gccatatttg gacaaatgcg aactacaaag
    22621 ttaattaatt agggaaaaaa cattacattt gcaccctatt ttacctgact tataaattaa
    22681 taataaaatt atgcaa
//
LOCUS       DAOSOF010000011        21589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2426_length_21589_cov_5.976595, whole genome shotgun sequence.
ACCESSION   DAOSOF010000011 DAOSOF010000000
VERSION     DAOSOF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_21589_cov_5.976595"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLM68_01315"
     CDS             <1..464
                     /locus_tag="PLM68_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOT54043.1"
                     /translation="GGLISLIFNTLFVVSFFSIFTGVLPFTLDIDQQFIAAILTIIGY
                     SLNDTVVIFDRIREYRTLYPKRDIWENINEALNSTLSRTVNTSATTLVVLLAIAIFGG
                     EVIRGFAVALAIGVAICPFTSVLVSTPIVYELWKRRHGREEKKAMKGKTAR"
     gene            complement(469..978)
                     /locus_tag="PLM68_01320"
     CDS             complement(469..978)
                     /locus_tag="PLM68_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54044.1"
                     /translation="MKVVFPIIATGEQITRMCEMASLIWHEAYRDMLPRKQIQYMLAL
                     FLKPSIIEEQIAREGYTYCFINTGGVDAGFFGVCPRLEGNSLFLSKIYLLREYRGCGL
                     FVQAMDEIVRLARLQGLPAVRLHVNKNNTRAIKAYIRSGFHITGSDVFDIGKGYVMDD
                     HILEYVIPG"
     gene            1033..2526
                     /locus_tag="PLM68_01325"
     CDS             1033..2526
                     /locus_tag="PLM68_01325"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT54045.1"
                     /translation="MKNKRLVPLVLFIALGSLSLQAQPISHSKDIKKIATRAQELWQV
                     PAMAVTVVKDGETIFAEGIGKLSIEKNAPKADEYTLFVNASTSKAFTAALLGMMVDRG
                     YVKWTDRVIDHLPDFQLYDPWVTQNYLVKDIMTHKTGFEPQALDAIPALGYGRDDLYS
                     MFRLVRPVTSFRTTYAYCNALYTVAARIVEKYTGLTWEEAIEKFIFQPLEMNHSTTGK
                     DHYFIAANFASGHRLNKTEEGLKVTPRKDREDSYAWLQAVSPAAFVMSNAYDMGQWMK
                     MWLGKGTYQDKQILTEETVNMLFSPQTICSWDNERVVLYAQGWRIEQGVQGKLINHTG
                     LAGGFTAWVVLVPELNLGVSVLMNQGSTTSPQTAIIRQVIDLFRGVESDWISKIYDDY
                     MRPADSRVPKVEEEYIQPLDNKVYMGTYFKEIFGESQVYEKNDTLYLKINDIDAPLRH
                     RNGNTFHFDARDESFDITFHVQDKLDWAVSYTIDFGDDLGSFVRVFK"
     gene            2523..3224
                     /gene="ispD"
                     /locus_tag="PLM68_01330"
     CDS             2523..3224
                     /gene="ispD"
                     /locus_tag="PLM68_01330"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT54046.1"
                     /translation="MKKSYNIAAIMAAGKGTRAGASLPKQYEELRGRLVLERAVDAFE
                     GHPDIDQVVIVADPDEMERVEKIIQNNSWKKVAGSVRGGKTRYQSSRAVIKAFSNRPE
                     DNLILHDAARPLVSARIISEVIEKLKHCRAVGVAVPVTDTLFFTGPGQSHIIRVPDRD
                     LFLRAQTPQGFRISVLGKAYEYAMKDPVFSATDDCGVVKRYLNNEKILLVPGDEKNMK
                     ITYPGDLEILAGWLE"
     gene            complement(3226..6813)
                     /gene="dnaE"
                     /locus_tag="PLM68_01335"
     CDS             complement(3226..6813)
                     /gene="dnaE"
                     /locus_tag="PLM68_01335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT54047.1"
                     /translation="MSSFVHLHVHTQYSILDGAAFIQQLFGKAEADKQPALAITDHGN
                     MFGVKEFLNTAANYPLVKPIVGCEVYLAPEGRLLKKNKEEATTCHLVLLAKNHEGYLN
                     LIKLVSQAWIDGVYYKPRIDHELLKQYHQGLIAMSACLAGELPRLVLAGKMDEASRLV
                     RWYKDLFGDDYYIELQRHKTRLTTPNAMECYTLQKQIEPHLVDLAGIHQVKLVATNDV
                     HFVNYEDAPAHDRLICVATNDDVSNENRKIRYTEEEYLKSGQEMTELFADIPQALATT
                     LEIADKVEPFSLNRKPILPHFPIPEVFSGADAYLRHLTYQGAERLYQAVSQELKERIE
                     MELGTISEMNFSDYFLIVADFIRAAREMDVWVGPGRGSSAGSVVAYCLGITLVDPIRY
                     GLLFERFLNPQRASFPDIDIDFADDGRGKVLQYVENKYGKDHVAHVITFGTMAARSAI
                     KDVARVQRLPLSEAERLAKLIPDRLPDKDNKPQAVTIENALKYVPELQAALKSDDPLV
                     SSTVEYARRLEGSVRNTGVHASAIIIGPDTLTNFIPLSTTKDKETGEDILVSQFEGSL
                     IEQVGMLKMDFLGLKTLSILKSTVENVRANHNISIDINALPLTDGPTYELFSRGETVG
                     VFQFESDGMRKWLRELKPTCIEDLIAMTALYRPGPMDYIPDFIDRKHGRKTISYDLPQ
                     MEKVLRETYGVTVYQEQVMLLSREIAGFSGLKADTLRRAMGKKKPEEMEKMKQDFLTG
                     GKNNGIDEAILKKIWVDWTAFAQYAFNKSHAASYSILSYQSGYLKTQYPAEYMAAVLS
                     GNRNNIEEVSKYMDECKRMEINVLGPDINESAHDFTVTRKGHIRFGMGGIKGVGLGAV
                     ESIIQTREQGGFYRDIYDFAERVNLNTCNRKVLESLVYAGAFDSFTDIKRHQYFLPCN
                     KDGVFLDSIMRYGARVQEEKNKDMTSLFGAGPDSLAPPERPVPPHMVPEAPEDLLKKE
                     KEMVGIYLSSHPLDKFSFEIKHFTSHNLLQIQEILDNNQHEEPFAPREVRLAGLVTGV
                     TSALARTSGRPWGTFTLEDYSASRKFTLFGKEYEKFLPYMREGEALLIKCTLQERDQY
                     KRKGQEAQEAGPKEKELRIQEILLLSNARESLQSITMDLGVEEISGTFRQELVQVLFR
                     NPGKITVHFVLKDKVNKMAVKVFSRSHRVDLTPDLLNWINKKGIAFTVE"
     gene            complement(6823..8343)
                     /gene="gpmI"
                     /locus_tag="PLM68_01340"
     CDS             complement(6823..8343)
                     /gene="gpmI"
                     /locus_tag="PLM68_01340"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT54048.1"
                     /translation="MKNVLLMILDGWGEGKQDRTNAIYTAGQPHIEKLRKAYPHSTLS
                     TSGEDVGLPDGQMGNSEVGHLNIGAGRIVYQDLVKINRACRDGSIRENKEVKAVYEYA
                     RHSGKALHLMGLVSEGGVHSSMEHLFTFLDMAHEYGLSKVYVHCLMDGRDTDPQSGKG
                     YVQKLEDHLARTTGKIASVTGRYYTMDRDKRWDRVKQGYDVAVHGIGAPFRKAVEGIA
                     ASYSQGVTDEFIKPVVCVDEAGIPVGTIGKDDAMIFFNFRNDRAREITRVLTQENMPE
                     YGMYTIPLYYCCLTPYDDTFKGLHILFDKENVRETIGEIISCNGLRQLRIAETEKYAH
                     VTFFFSGGREEVFPLEDRILVPSPKVATYDLKPEMSAPEVAEKIIIALNSKEYNFIVL
                     NFANGDMIGHTGVYEAICQAIQVIDRLAYQVTQTALENGYSVIITADHGNADNALNLD
                     GSPNTAHSMNRVPFIVVDKQVKEVQDGILADIAPTILHMMDIPTPAEMTGKSLIRK"
     gene            8403..10445
                     /gene="rnr"
                     /locus_tag="PLM68_01345"
     CDS             8403..10445
                     /gene="rnr"
                     /locus_tag="PLM68_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOT54049.1"
                     /translation="MSRRNNQKARSAGDYRKKQGPKTSRPVEYQGKLSMTREGFAFLL
                     VSTAEGEAPVDDIFIPARKLGGALHGDTVKIAVVPGKSRDGRKVEGAVTEILERSARP
                     YVGIVQVVDNQAYVITESRNMPFDIQVVKGGLNGAKTGQKVAVLVSGWDRKEQIPVGR
                     IVDVLGKPGDNTTEMHAILAEFGLPYRFEEQVEKEAGLLRDEILPEEIRERIDFRGVP
                     TFTIDPSDAKDYDDALSFRRTGPGDSLMEVGVHIADVSYYVDPGSLLDQAAYERGNSV
                     YLVDRTIPMLPEKLSNQLCSLRPHEDKLCFSAVFRFDQDHNVVSRWFGRTVICSACRM
                     SYEEAQAVLDKVIDVHTSSGPIPEPIREGLFFLHALAHKLRKERMHRGAIAFERPEVK
                     VQVDEKGVPLSIQVMELLDSHWLIEEFMLLANREVAEFINKKKDKKTGKAPTFVYRVH
                     EAPAEDKITAFRNFVHHFGYEMKQTKTVREYARELNRLLEKTKEKPESGGIIIMALRS
                     MARAHYTTNNVGHYGLAFDHYTHFTSPIRRYSDLMVHRLLLHYMNGGKNESQADMEAR
                     CTYISQREQLATEAERASIKYKMVEFMQDKVGREFDGVITGLTEWGIYVELEQTQVEG
                     MVPLRDIQDDYYVFDKETVTLTGTHNGTRYVLGQKVRIRVSRANLEQKQLDFILIQ"
     gene            10449..11036
                     /locus_tag="PLM68_01350"
     CDS             10449..11036
                     /locus_tag="PLM68_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT54050.1"
                     /translation="MKKGTIWIIIIAIIVLVVMWFVRINNRMVEAQEQAVQAWAQVEN
                     VYQRRADLIPNLVNTVKGAVDFEKSTLEAVIEARSRATSITVDPTNLTEESMAAFQDA
                     QDNLSQSLGRLMAVVERYPELKANQNFLELQAQLEGTENRIAVERRSFNQVVQAYNVL
                     IRKFPNSIVASIRNFESKAYFKAAEGAETPPVVAF"
     gene            complement(11043..12086)
                     /locus_tag="PLM68_01355"
     CDS             complement(11043..12086)
                     /locus_tag="PLM68_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOT54051.1"
                     /translation="MSEKKQPPKTPIRELGKYELIDRLSARFISSNEATLQGVGDDAS
                     VACFHEALTVSAQKMSLEGVDFDLSYFPLKFIGYKTAIHAMGQVMAMNAIPLQLLVST
                     GVSQRFFVEDMEELFMGVEAAARLYGADVTAIDIQSSYTGLSVAVTAVGEGNAGLLTR
                     RCGARDTDLICLSGSVGGAYMGLQLLQREKRVFNGEAGKDRLPDLKGHEYILEQYLKP
                     VLPAALLENLRKDDMVPTSMCLLYNGLSNGVLSIAKASGTGAKVYVDKIPIARECFEY
                     AQANGNDPSPLVAALNGGDDFQMLFTLPLTCYETLSKEYTLDIIGHICEAPAGCTLIT
                     PGGKSIPLESPGM"
     gene            12125..13573
                     /gene="sufB"
                     /locus_tag="PLM68_01360"
     CDS             12125..13573
                     /gene="sufB"
                     /locus_tag="PLM68_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOT54052.1"
                     /translation="MKENQNDILKKIAESEYEQGFVTDLEQEVFPKGLDENVIRQISY
                     RKEEPAWLLEFRLDAFKRWQTMKMPEWAHLTIPPIDYQDIIYFAAPRKGPENAGPGEM
                     DPALAETFDKLGIPLHERNVLAGMAVDAIFDSVSVKTTFKETLAEKGIIFCSFSEAVR
                     DYPSLVRKYLASVVPVGDNFFSALNSAVFSDGSFCYIPKGVRCPMELSSYFRINARGT
                     GQFERTLIVAEEGSYLSYLEGCTAPQRDENQLHAAVVEIVVMQDAEVKYSTVQNWYPG
                     DATGKGGIYNFVTKRGICKGTNSRLFWTQVETGSAITWKYPSTILQGDNSVSEFYSVA
                     VTNNYQQADTGTKMIHIGKNTRSHIVSKGISAGYSQNSYRGLVRILPGAENARNHSQC
                     DSLLLGDKCGAHTFPVIENSNESSVVEHEATTSKIGEDQLFYCMQRGIGPEDAVGLIV
                     NGYAREVLNKLPMEFAVEAQKLLSLSLEGTVG"
     gene            13573..14232
                     /gene="pnuC"
                     /locus_tag="PLM68_01365"
     CDS             13573..14232
                     /gene="pnuC"
                     /locus_tag="PLM68_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT54053.1"
                     /translation="MKEFFNLYNIFFVIGGQGISYLEFFAVLFGLTSVLLASLARSIN
                     YWIGFVYTALLFMMFLQKNLYTNMLLQPVSLGINIYGLYRWTRPRQDEKNKKNQLRIT
                     FLNNRQRVIYVFLLACIVLVWGFVLTKLHFISQAFPPARTPYLDSLVAGLMLMAQILA
                     AQKKWECWIFWVALNIGNVILYVSAGLVFMPIVAVCYLALNIIGVFHWKKEWEKQKML
                     C"
     gene            14226..14972
                     /gene="sufC"
                     /locus_tag="PLM68_01370"
     CDS             14226..14972
                     /gene="sufC"
                     /locus_tag="PLM68_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOT54054.1"
                     /translation="MLSIKDLHVKIGDKEILRGINLEIKQGEIHAIMGPNGSGKSTLA
                     AVLAGREMFTVTGGSAHFMGQDLFAMAPEDRARMGLFLGFQYPVEIPGLTNVNFMKAA
                     VNEQRKYKNLPPLSSAEFLKVMREKMNVVQMDPSFVGRGVNVGFSGGEKKRNEIFQMA
                     MLEPKLAFLDETDSGLDVDALRIVAQGVNQLKTEENAFVVITHYQRLLDYIVPDIVHV
                     IYKGQIIRTDGKELATEVETMGYDHIINQS"
     gene            14969..16006
                     /locus_tag="PLM68_01375"
     CDS             14969..16006
                     /locus_tag="PLM68_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOT54055.1"
                     /translation="MTQYTYMPEVRHVFRCTVPDGNIRQVFLVDGVLQELENDVKGVV
                     LEKGPDQMGLRMHISGDHSDEPIQIISVRSEDLPEPLEFSNYWELAAGASAQVIVCDH
                     TLHEHLFRTFRFSYIKLGQGARLDILFMQNEHEKSRYWLDLKADLGRDSQLDCHILSL
                     FGGTIYNKLYVSMPETGAACYLDGLYLADREQEIHYDISVRHESQGCQSRQLFKGILD
                     NQARGSFNGSIYVAPGAQKTEAYQANHNLLLTRQAKITTKPQLEIYADDVKCSHGATI
                     GRLDELGLFYLRSRGISLAEAQMIQQMAFVQDVLDRVRTSSIRSRLEKMVESRLRGEF
                     TRCSHCTMHCC"
     gene            16125..18674
                     /locus_tag="PLM68_01380"
     CDS             16125..18674
                     /locus_tag="PLM68_01380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOT54056.1"
                     /translation="MDSDVKNAKAIFPYEEALAKTKEYFKDDELAASVWINKYAMKDS
                     FGNLYECTPKDMHQRLAAEFARIEKKYPNPMSQEEIFHLLDHFKYVIPQGGPMSGIGN
                     DHQIASLSNCFVIGHERPADSYGGILKIDEEQVQLMKRRGGVGHDLSHIRPGGSPVLN
                     SALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAEQFIDAKLEQNKVTGA
                     NVSVKIDDDFMKAALSGKPYTQQYPTDAVTPRYKKEIDAAALWKKIICNAWKSAEPGV
                     LFWDTILRESVADCYADLGYKTVSTNPCGEIPLCPYDSCRLIAINLFSYVDRPFTPEA
                     RFNVTLFKNHVMKAMRLNDDLVDLEMEKIERIIDKLDKDPEDEVIKRTERELWLKIKE
                     KTLGGRRTGLGITAEGDMLAALGLRYGSEEAIQVGVQVQKTLALAAYRSSVIMAQERG
                     PFPIYDANRELRNPMIERIRQEDEELYKQMIKTGRRNISMLTIAPTGTTSLMTQTTSG
                     IEQVFMPVYQRRKKVNPNDKNVNVSFTDKLGDAFEEYNVFHHKFLDWAEINGYDRKRV
                     MQMSKEDLQKLVEESPYYKATSNDVDWVAKVKMQGQLQKWVDHSISVTVNLPSDISEE
                     MVAKVYKTAWEWGCKGITVYRDGSRTGVLVSADSPEKGGKMMKSMPKERPVELEAEVI
                     RFKNANEQWIAFVGLYEGRPYEIFTGKLDEDTRVIPKSITMGKVIKVVEPKGSRYDFS
                     FIDKYGYPNTVGGISHMFNQAYWNYAKLISGVLRNGLPVEEVVHLVSSLELDSQTINN
                     WRTGVERALKRYIPNGTKDSSGTECEKCGERNLVYQEGCLLCMSCGYSKCS"
     gene            18685..19467
                     /gene="ispE"
                     /locus_tag="PLM68_01385"
     CDS             18685..19467
                     /gene="ispE"
                     /locus_tag="PLM68_01385"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT54057.1"
                     /translation="MATTVAYPFSKINMGLHVLEKRADGYHNIETLFFPHSLSDILEI
                     TDVPGAPQDVQLFLYGLPVTGPYDANLCVKAYRLLAERYKLPPVHIHLYKQIPPGSGL
                     GGGSSDAAACLQILNKHYDLGLRHEELLSHAIRLGSDVPFFIQSTPPLPYFASGKGEV
                     LEPFSLDLSAYRIELRFPPVVISTAEAYTAIKPDSNRVPLRDLLRQPVDTWRDAIRND
                     FETFVFQKYPGTIQYKQSLYEEGAMYASMTGSGSAFFGLFKI"
     gene            19501..20142
                     /locus_tag="PLM68_01390"
     CDS             19501..20142
                     /locus_tag="PLM68_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT54058.1"
                     /translation="MGLYYQEQFKNGAQIVVWEITETEEQLQEICTLPNEEREELSYI
                     TNPQRRLERLAVRAILHTLFQEKVYLGYHENGRPFLQNSIIELSIAHTRRFACVLTHP
                     ENNVGIDIELLDRNFTAVEKKAIGDDEKNFLSDKPEVRKLQLALIWCAKEALFKYMSQ
                     PDVDYIEQMRIDKIVPRETGEIDAVFYQRDGIEEDFTLEYKVLDDHVMVWLVG"
     gene            complement(20120..21055)
                     /locus_tag="PLM68_01395"
     CDS             complement(20120..21055)
                     /locus_tag="PLM68_01395"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOT54059.1"
                     /translation="MDSFKPLTLIDQGIIKPYLAKAGKAASEYSFATLFMWSGIYHTD
                     FSVFSSLGDELLLIRSHRNLAEPFHYLYPMSLHGPVAAGTAVEILSAFSKDAPFSVGV
                     LSAEEAGQLAAREPAHVEVQSFRDTFDYIYNASDLALLTGKKFQAKRNHLNSFLNEYR
                     EFEARQIRLEDIPACLAMNDEWCRLMGCNQDVGLEQETCAVHRAFNSFGELGLEGLMI
                     LIDGKIVAYTLGELLTKNTWLVHVEKAFQEFRGAYQAINQLFVRYAMEKYPHVEYINR
                     EDDSGDMGLRIAKMSYHPAFFVEKYTALFNQPATP"
     gene            complement(21065..>21589)
                     /locus_tag="PLM68_01400"
     CDS             complement(21065..>21589)
                     /locus_tag="PLM68_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54060.1"
                     /translation="DSPTLPFFIQASRELGYYGYDTKPFRFKTTRTRFIPEKAKKGLF
                     SKQEKPEKEAEQDTVKKQVAGKWVTKKYPAMELKSAERYLKDLFLPDFYKPFFNKSMY
                     RLQRDFVKKSDARLVFIYGEWDPWTAAAVPDPGKQNVLYYVQPGGSHRARIGTLPQSM
                     REALLEQLQSWLNE"
BASE COUNT         5709 a         5055 c         5495 g         5330 t
ORIGIN      
        1 taggtgggct tatatccctg attttcaata cgctttttgt tgtttctttc ttctccatat
       61 ttaccggggt gttacctttc acactggata ttgaccaaca gttcatagcg gccatactga
      121 ccattatcgg atactcgctg aacgacacgg tggttatctt tgacaggatc agggaatacc
      181 gtaccctgta tcccaaacgg gatatctggg aaaacatcaa cgaagcattg aattctaccc
      241 tttcccgcac tgtcaacaca tcggccacaa cgctggtggt tttgcttgcg attgccatct
      301 ttggcggaga ggtgatccgc ggttttgcgg tagctctggc tataggggta gccatctgtc
      361 ctttcacatc ggtcctggtt tccactccta ttgtatacga attgtggaaa aggcgccatg
      421 gcagggaaga gaaaaaagcc atgaagggaa aaaccgccag gtaaaggatc atcccggaat
      481 tacgtattcc aggatatgat catccatgac gtaccccttg ccaatatcaa atacatccga
      541 tccagtgata tgaaagccgg accggatgta tgcttttatg gccctggtgt tgtttttgtt
      601 cacatgcagc cttactgccg gtaagccctg aagacgggcc agccgtacta tttcatccat
      661 ggcctggaca aatagtccac atccgcggta ttctctgaga aggtatattt tactaagaaa
      721 caaagaattc ccttccagcc gggggcagac cccgaaaaag cccgcgtcaa cgccgcccgt
      781 attgataaaa caatacgtgt atccttcacg ggcaatctgt tcttcaatga ttgaaggttt
      841 caaaaacaac gccagcatgt attggatttg tttccggggc aacatgtccc ggtaggcttc
      901 atgccagatc agggaagcca tctcacacat gcgggttatc tgctcacctg tggctataat
      961 cggaaaaaca accttcatgg caggtaaaaa taaggttttc tttgtatttt tgttgaaact
     1021 aaagacataa aaatgaaaaa taaacggtta gtccctctgg tcctgttcat tgccctgggc
     1081 agtttatccc tccaggccca accaatttca catagtaaag acataaaaaa aatagccacc
     1141 cgggcacagg aactctggca ggtgcctgct atggctgtta cagtagtcaa agatggggaa
     1201 accatttttg ctgaaggtat aggaaagctg agcatagaaa aaaatgcacc gaaagccgat
     1261 gaatatacgt tatttgtgaa cgcctccaca agcaaagcgt ttacagccgc cttgctgggc
     1321 atgatggtgg acaggggcta tgtgaaatgg acagaccggg tcattgacca ccttcccgat
     1381 ttccaattgt acgacccctg ggttacacaa aattacctgg ttaaggacat catgacacat
     1441 aagaccggtt ttgagccaca ggcgctcgat gcgattccgg ccctgggtta cggcagggat
     1501 gatctttaca gcatgttcag gctggtcaga ccggtcacca gcttcaggac aacctatgct
     1561 tattgcaatg ccttgtacac cgtggccgcc aggatcgtgg aaaaatatac ggggcttacc
     1621 tgggaagaag ccatagaaaa gtttatattc cagccccttg agatgaatca ttcaaccaca
     1681 gggaaggacc attatttcat agcagcaaat tttgccagcg gccatcggct gaacaagacg
     1741 gaagaaggat tgaaagtcac cccgcgcaaa gacagggaag attcttacgc ctggcttcag
     1801 gcggtctcac ctgcggcttt tgtgatgtcc aatgcgtatg acatgggaca atggatgaaa
     1861 atgtggctgg gtaaaggcac ttaccaggac aaacagatcc ttacagaaga aacagtaaac
     1921 atgctttttt cacctcaaac gatttgttcc tgggacaatg agcgggtggt gctttatgca
     1981 cagggatgga ggattgaaca gggtgtacag ggaaaactca tcaaccacac aggactggcc
     2041 gggggattca cggcctgggt cgtccttgta cctgagttga acctgggggt cagtgtactg
     2101 atgaaccagg gatcaaccac ctcacctcag acagccatta tcaggcaggt catagatctg
     2161 ttcaggggtg tcgaatcgga ctggatatca aagatatatg acgattacat gcgcccagcg
     2221 gactcccgcg taccgaaagt agaggaagaa tatattcaac cacttgataa taaagtttat
     2281 atggggactt atttcaagga aatattcgga gagtcgcagg tgtatgagaa aaacgacaca
     2341 ttatatctga aaatcaacga catagatgcg ccattacgcc ataggaacgg caacacattc
     2401 cactttgatg caagggatga aagctttgac atcacttttc atgtacaaga caaattggac
     2461 tgggccgtga gttataccat tgactttgga gacgacctgg gctcattcgt aagggttttc
     2521 aaatgaagaa aagttacaat atagcagcaa tcatggccgc tggtaaagga accagggcag
     2581 gagcctctct tcccaagcaa tacgaagagt tgcgcggaag gcttgtgctg gaaagggcgg
     2641 tagatgcatt tgaaggacac cccgacatag accaggtggt cattgtggca gatccggatg
     2701 aaatggaaag ggtagagaag atcatccaaa ataactcctg gaaaaaagtc gccgggtctg
     2761 tcaggggagg aaaaacccgt taccagtcat cacgtgcagt catcaaggcg ttttctaaca
     2821 gaccggaaga caacctgata cttcacgatg cagccagacc tctggtaagc gccaggatca
     2881 tatcggaagt cattgaaaaa cttaaacatt gccgtgcagt aggggtggct gtgcctgtaa
     2941 cagataccct gttcttcacg ggccccggtc aaagccatat catcagggta cccgaccggg
     3001 atctttttct aagggcacaa acaccccagg gcttccggat ttccgtgttg ggcaaagctt
     3061 atgagtacgc catgaaagat cctgttttct cggccacgga cgattgcggt gtggtcaaaa
     3121 ggtatttgaa caatgaaaaa atcctcctgg tgccgggaga tgaaaagaac atgaaaatca
     3181 cgtatcccgg ggacctggag atccttgccg gctggctgga gtaagttatt cgacggtaaa
     3241 tgcgatacct tttttgttga tccagttaag aaggtccggg gtcagatcga cacggtgtga
     3301 acgggagaat actttaaccg ccatcttgtt gactttgtct ttcagtacga aatgtaccgt
     3361 gattttgccc gggttccgga acaaaacctg gaccagttcc tgacggaaag ttccggatat
     3421 ctcttccacc ccaagatcca tggtgataga ctgcagtgat tccctggcat tagagagtag
     3481 taaaatttcc tggatacgga gctctttttc ttttggaccg gcctcctggg cttcctgacc
     3541 ttttctttta tactgatcgc gttcctgcag ggtgcatttg ataagcagtg cttcgccctc
     3601 gcgcatgtag gggaggaatt tctcatattc tttcccgaac agggtgaatt tcctgctggc
     3661 cgaataatct tccagtgtaa aagtgcccca gggcctgccc gatgttctgg ccagggcaga
     3721 ggtcacaccg gtcacaaggc cggcaaggcg tacttctctg ggggcaaacg gctcctcatg
     3781 ttggttatta tccagtatct cctgtatttg caggaggtta tggctggtaa agtgttttat
     3841 ctcaaagctg aacttatcca gcgggtggga tgacaggtat atcccaacca tttctttttc
     3901 ctttttcagc aaatcttccg gagcttcagg aaccatatga ggtggcaccg gtctttcggg
     3961 aggggccagt gaatcgggac cggcaccgaa caagctggtc atgtctttat ttttttcctc
     4021 ctggacccgg gcaccgtaac gcattatact gtccagaaaa acgccgtcct tattgcaggg
     4081 caggaagtat tggtgtcttt ttatatctgt gaaagaatca aaagcccccg catacacaag
     4141 gctttccagc actttcctgt tacaggtgtt caggttgacc cgttctgcaa agtcatagat
     4201 atcccgatag aaaccacctt gttctctggt ctggataatg ctttcaaccg ccccgagtcc
     4261 cacgccctta ataccgccca taccaaaacg gatatgtccc ttacgggtta cagtgaaatc
     4321 atgtgcagac tcattgatat caggtccaag tacgttgatt tccatacgtt tgcactcatc
     4381 catatacttg gatacttcct cgatattgtt ccggttcccc gagagcaccg cagccatgta
     4441 ctcagcggga tattgtgttt taagatagcc cgactgatag ctgagtatgg aataactggc
     4501 tgcgtgtgac ttgttgaacg catattgagc aaaagcagtc caatcgaccc atatcttctt
     4561 caggatggct tcatcaatgc cgttgttttt tcctccggtc agaaaatcct gcttcatctt
     4621 ttccatttcc tccggtttct tctttcccat ggctctgcgc agggtgtcag cctttaatcc
     4681 tgaaaaaccg gcaatttccc tggacaacaa catgacctgc tcctggtata ccgttacacc
     4741 gtaggtttcc cgaagaactt tttccatctg cggcaggtca taggaaatgg ttttacggcc
     4801 gtgctttcgg tcaatgaaat cgggaatata gtccatgggt ccgggcctgt aaagggcggt
     4861 catggctatc aggtcttcaa tgcaggtggg tttgagctcc cggagccatt tacgcatacc
     4921 gtcgctttca aactggaaca cacccacggt ttcaccccgg ctgaacagct cgtaggtggg
     4981 cccgtctgta aggggaaggg cattgatgtc gatggatatg ttgtggtttg ccctgacgtt
     5041 ttctacggta ctcttgagga tgctgagcgt tttcagtccc aggaaatcca ttttcagcat
     5101 gcccacctgt tcaatgagcg atccttcaaa ctgggaaacc aggatatcct cccctgtttc
     5161 cttatcttta gttgttgaca ggggaatgaa attggtcagg gtatcgggcc cgataatgat
     5221 agcactggca tgaaccccgg tgttccttac agaaccttcc aaccggcggg catattcaac
     5281 cgtcgagctt acaaggggat catccgattt gagggcagct tgcagttcgg gcacgtactt
     5341 cagtgcgttt tcaatggtta cggcctgggg cttgttgtct ttgtcgggca gacggtccgg
     5401 gatgagtttg gccagccgct ctgcttccga cagtggaagg cgctgtaccc gggcaacatc
     5461 ttttatcgca ctgcgggctg ccatggttcc gaatgtgatc acatgcgcaa catggtcctt
     5521 cccgtatttg ttttccacat actgcaacac cttgccacgg ccgtcatcgg caaaatctat
     5581 gtcaatatcg ggaaatgaag ccctttgtgg attcagaaaa cgttcaaaca acaaaccgta
     5641 gcgaatagga tctaccagcg tgattcccag gcagtatgcc acaaccgatc ctgcagagga
     5701 accccttccg gggcccaccc atacgtccat ttcccgggca gcccgtataa aatccgccac
     5761 aatcagaaag taatccgaaa aattcatctc ggaaatagtt cccagttcca tctcaatacg
     5821 ttctttcagt tcctgggata ctgcctggta aagacgttcg gctccctggt aagtaaggtg
     5881 ccttaggtag gcatcggcac cggaaaacac ttctggaatt ggaaaatgag gcaggatggg
     5941 cttcctgtta agactgaagg gctccacctt atcggcaatc tcaagcgttg tggccaatgc
     6001 ctggggtatg tcggcgaaca attcagtcat ttcctgccct gacttcaggt attcttcctc
     6061 tgtatagcgg atcttgcggt tttcgttgga gacatcgtca tttgtagcca cacagataag
     6121 ccggtcgtgc gcaggcgcat cttcataatt gacgaagtga acatcattgg tggctaccag
     6181 tttcacctgg tgtatccctg caagatctac aagatggggc tcgatctgtt tctgcagggt
     6241 atagcattcc atggcgttcg gggtggtcag ccttgttttg tgacgctgaa gctcaatata
     6301 atagtcgtct ccgaacaaat ccttgtacca tcttacaaga cgagatgcct catccatttt
     6361 cccggcaagg acgaggcgcg gcaattctcc ggccaggcag gcactcatgg cgatcaatcc
     6421 ctggtggtat tgtttgagca gctcatgatc tatgcgcggt ttatagtaaa ccccgtctat
     6481 ccaggcctgt gaaacgagtt ttatcaggtt caaatatcct tcatggtttt tggcaagcag
     6541 caccaggtgg caggtggtgg cttcttcttt gttcttcttc aacaatctgc cctcaggggc
     6601 caggtagact tcacacccga cgatgggttt gaccaaaggg taattggctg ctgtattgag
     6661 aaactccttt acaccaaaca tgtttccatg atcggttatg gctagcgcag gttgtttgtc
     6721 ggcttctgcc ttgccaaaaa gctgctggat aaaagcagct ccgtcaagaa tggaatattg
     6781 tgtgtgaaca tgcaggtgaa caaaggagga catgatggat aattatttac ggatcagtga
     6841 tttgcctgtc atttcagccg gggttggaat atccatcata tggagtatgg tgggggcgat
     6901 atcggccagg ataccgtctt gcacctcttt cacttgcttg tcaaccacaa tgaacgggac
     6961 ccggttcatg gagtgggccg tattgggaga cccgtcaaga ttcaacgcat tgtctgcgtt
     7021 gccatgatca gccgtaatga taaccgaata accgttttcc agggctgtct gggtaacctg
     7081 gtaagccagc cggtcaataa cctgtatcgc ctggcagatg gcttcatata cccctgtatg
     7141 gccaatcata tccccgttgg caaaattgag aacaatgaaa ttgtattctt tgctgttcaa
     7201 ggcgatgatg atcttttcgg ccacttcggg tgcgctcatt tcgggtttca gatcataagt
     7261 ggccactttg ggagaaggga ccaggattct gtcctccaga ggaaaaacct cttcccgtcc
     7321 tccagaaaag aaaaaggtca cgtgcgcgta cttctcggtc tcggcaatcc tcaactgcct
     7381 taagccatta cagctgatga tctcgcctat ggtttcccgt acgttctctt tgtcaaaaag
     7441 gatatgaaga cctttaaagg tgtcatcgta gggagtcagg cagcaataat acagggggat
     7501 ggtatacatt ccgtattcag gcatgttctc ctgggtcagc acccgggtga tctcacgggc
     7561 gcggtcattc ctgaaattga agaatatcat ggcgtcgtct ttccctatgg tcccaacagg
     7621 aatacccgct tcatccacac agacaacagg tttgataaac tcatcggtaa caccctggct
     7681 gtaactggct gctattcctt caacagcttt tctgaatggt gcgcctatgc cgtgaacagc
     7741 cacgtcatag ccttgtttga cccggtccca gcgtttgtcc cggtccatgg tataataacg
     7801 cccggtaaca ctggcaattt ttccagtagt gcgggccagg tggtcctcca gtttttgcac
     7861 atatcccttt ccgctctggg ggtcggtatc acgaccgtcc atcaggcaat ggacatagac
     7921 ctttgacaag ccgtattcgt gtgccatgtc aagaaaagta aagagatgct ccatggagga
     7981 gtgtacacct ccctcagata caaggcccat caggtgcagc gccttgcccg aatgcctggc
     8041 atattcgtaa acggctttta cttccttgtt ttcacgaatg gacccatccc ggcaagccct
     8101 gttgatcttg acaaggtcct ggtatacaat gcgtcctgcg cctatgttca ggtgtccgac
     8161 ctcagaattt cccatttgtc catcgggcag gcccacgtct tcacccgagg tgctcagtgt
     8221 actgtgagga taagccttac ggagcttttc aatatgtggc tgacctgcag tatatattgc
     8281 attggtgcgg tcctgttttc cttctcccca gccatccagt atcattaaaa gtacattttt
     8341 catagaagca tagattatcc tgcaaaggta cgaaaagaat ttgtgtacct ttgccaaata
     8401 ttatgagcag gagaaacaac caaaaagcga gatctgcagg tgactacaga aagaaacaag
     8461 gcccgaagac atcccggcct gttgagtacc agggaaaatt aagcatgaca agggaaggtt
     8521 ttgccttcct gcttgtctct acggcggagg gagaagctcc ggtggatgac atattcatcc
     8581 cggcaaggaa attgggagga gcgttgcatg gggacacagt gaaaattgcc gtggttccgg
     8641 gaaaatcaag agacggacgc aaggtggaag gtgctgtgac cgaaatcctg gaacgctccg
     8701 caaggcccta tgtgggcatt gtccaggtag ttgataacca ggcctatgtt attacagaat
     8761 ccaggaatat gccctttgac atacaagtgg tcaaaggagg actgaacggg gcaaaaaccg
     8821 gtcagaaagt tgctgtactg gtgtccggat gggacaggaa agaacaaata cccgttggaa
     8881 gaattgtcga tgtgctgggc aaacccggtg acaatacaac tgaaatgcac gccatcctgg
     8941 ctgaattcgg gcttccctac aggttcgaag aacaggtgga aaaagaggcc ggattgttgc
     9001 gggatgaaat actacccgaa gaaattaggg aacggataga tttcaggggt gttccgactt
     9061 tcaccataga cccctccgat gcaaaggatt atgacgatgc cttgtctttc agaagaaccg
     9121 gaccgggaga ctcactcatg gaagtggggg tgcatattgc cgatgtaagc tattatgtgg
     9181 acccgggatc cctgctggac caggcggcct atgaacgcgg aaactcagtt tacctggtag
     9241 acaggaccat ccccatgctc ccggagaagt tatccaacca gctttgctcc ctgcgacccc
     9301 atgaagacaa actttgtttt tctgcggttt tccgttttga tcaggatcat aatgtggttt
     9361 cacgctggtt cgggcgcacg gtgatctgtt ctgcctgccg catgtcctat gaagaggcac
     9421 aagctgtttt ggataaggtg atagatgttc atacaagttc agggcccatt ccggaaccca
     9481 tacgggaggg actctttttt cttcatgcac tggcacacaa gctgagaaag gaacggatgc
     9541 acaggggtgc tatcgctttt gaaaggcccg aggttaaggt acaggtggat gaaaagggag
     9601 ttcccttgag catacaggtg atggaactgc tggattccca ctggctcata gaggaattca
     9661 tgctgctggc aaaccgcgaa gtggccgaat tcatcaataa aaagaaagat aagaaaaccg
     9721 ggaaggcccc gacattcgtg taccgggtgc acgaagcccc ggcagaagac aaaataacag
     9781 ctttccggaa ttttgtgcac catttcgggt atgaaatgaa acagaccaaa acggtacgtg
     9841 agtatgccag ggaactgaac cggcttttgg aaaaaacaaa ggaaaaaccg gagtcgggag
     9901 gaatcattat catggcgctg cgttccatgg cacgggccca ctatacgaca aacaacgtgg
     9961 gtcactacgg cctggccttt gatcactaca cccatttcac atctcccata cgccggtatt
    10021 cggatctgat ggtacacagg ctgctgctgc attatatgaa tggaggaaag aatgaatccc
    10081 aggcagatat ggaggcacgt tgtacatata tttcacaacg ggaacaactg gcaacggagg
    10141 cagagcgcgc aagcatcaaa tacaaaatgg ttgaattcat gcaagataag gtgggccggg
    10201 agtttgacgg cgtcataacc ggtctcacgg agtgggggat atacgtggaa cttgaacaga
    10261 cacaggttga ggggatggtg cccttgcgtg acatccagga tgattactat gtttttgata
    10321 aagaaacggt tactcttacc ggtacacaca atggcacccg ctatgtactg ggtcagaagg
    10381 tacgcatacg agtttcaagg gcaaatcttg aacaaaagca attggatttt attttaatac
    10441 agtaaacaat gaaaaaaggc acaatctgga ttattatcat cgccatcata gtgttggtgg
    10501 ttatgtggtt tgtcaggatc aacaacagga tggttgaagc ccaggaacag gctgtccagg
    10561 catgggccca ggtagagaat gtctatcaac gccgtgcaga cctgatcccc aacctggtca
    10621 ataccgtcaa aggggcagtg gattttgaaa agagcacttt ggaagcagtc atagaagccc
    10681 gctcacgtgc cacgtcaatc acggttgacc ccactaatct tacagaagaa agcatggccg
    10741 cctttcagga cgcgcaggac aacctgagcc agtcactggg gcgtctcatg gcagttgtgg
    10801 aaagatatcc cgagctcaag gcgaatcaga acttcctgga actgcaggca cagctggaag
    10861 gtacggaaaa caggattgcc gtggaaagac gttctttcaa ccaggtggtt caggcttaca
    10921 acgtgctgat ccggaagttc ccgaattcca ttgttgccag cataaggaat tttgaaagca
    10981 aggcttattt caaagcggcc gaaggagcag aaacccctcc tgtggtagca ttctgataaa
    11041 aattacatac cggggctttc caacggaatg gactttccac cgggggtgat taatgtgcaa
    11101 ccggccggag cctcgcagat gtgacctatg atatccaggg tgtattcttt gctcagggtc
    11161 tcgtaacagg taagcggcag ggtaaaaagc atctgaaaat catctcctcc gttgagagcg
    11221 gccacaagcg gggaagggtc gttcccgttt gcctgagcat attcaaaaca ttcccgggca
    11281 atggggattt tatccacata tacttttgcg cctgttcccg aagcttttgc aatgcttaaa
    11341 acaccattcg ataaaccatt gtatagcaaa cacatggatg ttggcaccat gtcatctttt
    11401 ctcaggtttt ccaggagtgc tgccggcaag acgggtttca gatattgttc caggatgtat
    11461 tcatgtccct taaggtcagg aagcctgtct tttcctgcct ctccgttaaa aacccttttc
    11521 tcgcgctgca gcagctgcaa acccatataa gctccgccaa cactgccgct aagacagata
    11581 aggtcggtat cgcgggcacc gcagcggcgt gttagcagtc cggcatttcc ttcccctacc
    11641 gcggtgacag ccacgctcaa tcccgtatag gaagactgga tatcaatagc ggtgacatcc
    11701 gccccgtaca gccgcgcagc tgcttcaacg cccatgaaca gctcctccat gtcctccacg
    11761 aaaaaacgct gggaaacacc tgtgctgaca agcaattgga gcgggatggc attcatggcc
    11821 ataacctgcc ccatggcgtg aatggcggtt ttgtaaccta tgaatttcag ggggaaatag
    11881 ctaaggtcga agtccactcc ctcaagagac attttttgag ccgaaacggt cagtgcttca
    11941 tgaaaacagg cgaccgaggc gtcgtccccc acgccttgaa gcgttgcttc attacttgaa
    12001 ataaaacgtg ctgaaagcct gtcaatcaac tcgtatttac ccaattcacg aatgggtgtt
    12061 ttcggaggtt gttttttctc gctcatacca caaaagtacg gaaaagatgg tatatttgcg
    12121 ggctatgaaa gaaaatcaaa acgacatact taaaaaaatt gcagaaagtg agtatgagca
    12181 aggttttgtg acggatctgg aacaggaggt ttttccgaaa gggctggacg aaaacgttat
    12241 caggcagata tcttacagaa aagaagaacc cgcatggctg cttgagtttc gtctggatgc
    12301 attcaaaagg tggcagacga tgaaaatgcc tgaatgggct catttgacaa taccacccat
    12361 tgattaccag gatattattt attttgcagc accaagaaaa ggacccgaga acgccggtcc
    12421 cggggaaatg gatcccgccc tggccgaaac attcgacaaa ctgggcatac cccttcacga
    12481 aaggaacgta ttggccggca tggctgtgga cgccatattt gattctgtct ctgtaaaaac
    12541 aaccttcaag gagacccttg ccgagaaagg gatcatcttt tgttcttttt cagaggcagt
    12601 aagggattat ccatcccttg taagaaagta tctggccagt gtggtgcctg tgggagacaa
    12661 tttcttttcc gctttgaatt ccgcggtatt ttccgatgga tcattctgtt atattccaaa
    12721 aggagtgcgt tgtcctatgg agttgtccag ctatttcaga ataaatgccc gtggcacagg
    12781 tcagtttgaa aggacgctca tagtggccga agagggatcg tatctgagtt acctggaagg
    12841 atgtactgct ccgcagcgcg atgaaaacca acttcatgca gctgtggtgg aaattgtcgt
    12901 gatgcaggat gccgaggtga aatactcaac ggtgcagaat tggtatcccg gagatgctac
    12961 agggaaaggt ggcatttaca actttgttac caaaagaggc atctgcaaag ggaccaattc
    13021 ccggttattc tggacacagg tggaaacagg gtctgccatc acatggaaat acccgtccac
    13081 cattttgcag ggagacaatt ctgtaagcga attttattcg gtggcagtga caaacaatta
    13141 ccagcaggcc gataccggaa cgaaaatgat ccatatcggg aaaaacacac gcagccatat
    13201 agtaagcaag ggcatatcgg ccggttacag ccagaattca taccgcggac tggtcaggat
    13261 attgccaggg gcagaaaacg caagaaatca ctcacaatgt gactccctgc ttcttggaga
    13321 caaatgcgga gcccatacgt tccccgtaat agagaacagc aatgagagct ccgtggtgga
    13381 acacgaggca accacttcaa agatcgggga ggaccagctt ttttactgta tgcagcgtgg
    13441 aatcggcccg gaagatgccg tgggactgat agtgaacggc tatgcccggg aagtgttgaa
    13501 taagttaccc atggaatttg ccgttgaggc gcaaaaactg ctaagcctct cgctggaggg
    13561 gactgttgga tgatgaaaga gttttttaac ctgtacaaca tttttttcgt tataggaggc
    13621 caaggcatat cttaccttga attttttgcc gtcctcttcg ggctgacctc ggtgctgctg
    13681 gccagcctgg cacgttccat taattattgg ataggctttg tgtacacggc cctgcttttt
    13741 atgatgtttc ttcagaaaaa cctgtatacc aacatgttat tacagcctgt ttcactgggg
    13801 atcaacatct atggattgta ccgctggacc aggccccgtc aggatgaaaa gaacaagaaa
    13861 aaccagctca ggataacgtt tctcaacaac cggcaacggg tcatatatgt tttcctgtta
    13921 gcctgtatag tgctggtctg gggatttgtt ctgacaaaac ttcatttcat ttcacaagcg
    13981 tttccacccg caaggacacc gtatctggac tcgttggtag ccggtctgat gctcatggcg
    14041 cagatcctgg cggcccaaaa aaaatgggag tgctggattt tctgggtggc cttgaacata
    14101 gggaatgtaa ttctgtatgt gtctgctgga ctggtattta tgccaatagt tgctgtatgc
    14161 tacctggcct tgaatattat tggggtgttt cactggaaga aggagtggga aaaacaaaaa
    14221 atgttatgtt aagcattaaa gatttacatg taaaaatcgg ggacaaggaa atcctccgtg
    14281 gaataaacct ggaaataaag cagggagaga ttcacgcgat catgggaccg aacgggtccg
    14341 ggaaatccac cctggcggct gtgctggcag gcagggaaat gtttactgtt accggaggat
    14401 cagcccattt tatgggccag gatttgtttg cgatggctcc ggaggacagg gcacgaatgg
    14461 ggctattcct cggattccag tatccggtgg agataccggg tctgaccaac gtgaatttta
    14521 tgaaagctgc cgtcaacgaa caaagaaaat ataaaaacct gcccccgttg tcttcagcgg
    14581 aatttctcaa ggtcatgcgc gaaaaaatga atgttgtgca gatggatccc tcttttgtgg
    14641 gcaggggggt gaatgtgggt ttttcggggg gagagaagaa gagaaatgaa atatttcaga
    14701 tggccatgct ggagccgaaa cttgcttttc tggacgaaac cgatagcggg ctggatgtgg
    14761 acgcactgcg tattgttgcg cagggtgtca atcaattgaa aacagaggaa aatgcttttg
    14821 tggtcataac acactaccag cgcctgctcg attatattgt gcccgatatt gtgcacgtga
    14881 tatacaaggg tcagattatc aggacggacg gaaaggagct agccactgaa gttgaaacca
    14941 tgggatacga tcacattatt aatcagtcat gacacaatat acctatatgc cggaagtaag
    15001 gcatgttttc cgctgtactg ttcccgacgg gaatatcagg caggtattcc tggtggacgg
    15061 ggtcttgcag gagctggaga acgatgtgaa aggtgtcgtt cttgaaaaag gaccagacca
    15121 gatgggcctg cggatgcata tcagcgggga ccattcagac gaacccatcc agatcatcag
    15181 cgtgcgtagc gaggacctgc ccgagccgct tgagttttcc aattactggg aacttgcagc
    15241 tggcgcatct gcacaggtga tagtctgtga tcatacgctg catgaacacc tgttcaggac
    15301 attccgtttt tcttatatca aactcggcca gggggcaagg ctggatatac tgttcatgca
    15361 gaatgaacac gagaagtcca ggtactggct tgatctcaaa gcagatcttg gcagggattc
    15421 gcaactggat tgccatatcc tgagcctttt cggagggacc atatacaaca agctgtatgt
    15481 gtctatgccg gagacgggag ctgcctgtta cctggacggt ctttacctgg cagacaggga
    15541 gcaggaaatc cattacgata tatcagtacg tcacgagagc caggggtgtc aatcgcgcca
    15601 gctgttcaag ggcatcctgg acaaccaggc acggggttca ttcaacgggt ccatctatgt
    15661 ggctcccggt gcgcaaaaaa cagaagcata ccaggcaaat cacaacctgc tcctgacccg
    15721 tcaggcaaag atcaccacca agcctcagct ggagatctat gcagatgatg tgaaatgttc
    15781 acatggggcg accattggac gcctggacga gctgggtctc ttttatctcc gttcacgggg
    15841 gatttccctg gcagaagctc agatgataca gcagatggct tttgtccagg atgtgctgga
    15901 ccgtgtacgc accagttcca tcaggtcaag attggaaaag atggtggaaa gcaggttacg
    15961 cggcgaattc accagatgtt cccattgcac catgcactgt tgttaaaatt ttgtgcatat
    16021 ctttttatgt gtttgctgtt aatcacggtc gtttaaacta acttgcaatt cccttttccg
    16081 atgaggaaaa acctttaatt agtaaccttc ttatttatag cgtgatggat tctgatgtaa
    16141 agaatgcaaa ggccattttt ccctatgagg aagctttggc caaaacaaag gagtacttca
    16201 aagatgatga actggccgcc tcggtctgga tcaacaaata tgccatgaaa gactcttttg
    16261 ggaaccttta cgaatgcacg cccaaagaca tgcaccagag gctggccgcg gaatttgcca
    16321 ggatagagaa gaaatatccg aatcccatga gccaggagga gatatttcat ttgctggatc
    16381 atttcaaata cgtcattccg cagggaggcc ccatgtctgg tatagggaac gaccatcaga
    16441 tagcttcttt gtccaattgt tttgtgatcg gccatgaaag acccgctgat tcctacggag
    16501 gaatactcaa gattgatgaa gaacaggttc agttgatgaa acgccgggga ggcgtggggc
    16561 acgatctgtc tcatatccgt cccgggggat ctccagtgct taactcggcc ctgacatcaa
    16621 caggggtggt tccctttatg gaaaggtatt ccaactcgac acgggaagtc gcgcaggacg
    16681 gaaggcgcgg ggcgctcatg ctctccatat ccataaaaca ccccgacgcg gaacagttca
    16741 ttgatgccaa gctggaacag aacaaggtga caggagccaa tgtatcggta aagattgatg
    16801 acgatttcat gaaggctgcc ttgtcaggga aaccatatac ccagcaatac ccgacagacg
    16861 ctgtcactcc gaggtataaa aaggaaatcg atgccgctgc cctgtggaaa aagattatct
    16921 gcaacgcctg gaaatcggca gaacccggag tactcttctg ggataccata ctgcgtgaat
    16981 ccgttgcaga ctgctatgcg gacctggggt acaaaacggt cagcaccaat ccctgcggtg
    17041 aaataccact ctgtccttat gattcctgcc gtctgatagc catcaacctg ttctcatacg
    17101 tggacaggcc ctttacacca gaggcaaggt tcaatgtcac ccttttcaag aaccacgtga
    17161 tgaaagcaat gcggctgaat gatgacctgg tggacctgga gatggaaaag attgaaagga
    17221 tcatagacaa gctggacaag gatcccgaag atgaagtgat caagaggaca gaaagggaat
    17281 tgtggctcaa gatcaaggaa aaaacgctgg gaggcaggcg taccggcctg ggcattacgg
    17341 cagaaggaga tatgctggca gcccttggcc tgcgctatgg atcggaagag gccatccagg
    17401 tgggagtgca ggtacagaaa acactggccc tggcagcata tcgctcttcg gtgatcatgg
    17461 cacaggaaag aggacctttt cccatatacg acgccaaccg ggaattgcga aatcccatga
    17521 ttgagcgtat acgccaggaa gatgaggaac tgtataagca gatgataaag accgggcgaa
    17581 ggaatatttc catgttaacg atagccccga ccggtaccac aagcctgatg acacaaacca
    17641 ccagtggaat tgaacaggtg ttcatgcctg tatatcagcg tcgtaaaaaa gtcaatccaa
    17701 acgataagaa cgtaaacgta tcgtttaccg ataaactggg agatgcattt gaagaataca
    17761 atgtgttcca ccacaaattc cttgactggg cagagatcaa cggctatgac cggaaaaggg
    17821 taatgcagat gagcaaggag gacctgcaga agctggttga ggaatcgccc tactataaag
    17881 cgacatcaaa tgatgtcgac tgggtggcca aagtcaagat gcagggacaa ttgcagaaat
    17941 gggtggatca ttccataagc gttacggtga acctgccttc cgacatatcg gaagaaatgg
    18001 tggccaaagt atacaaaact gcctgggaat ggggatgcaa gggcataaca gtataccgcg
    18061 acggaagccg tacgggagtg ctcgtatctg cagattcacc tgagaaaggc gggaaaatga
    18121 tgaagtccat gccaaaggaa agaccggtgg aactggaggc ggaagtcatc cggttcaaga
    18181 atgccaatga acaatggatc gcctttgtgg ggctgtatga gggaaggcct tacgagatct
    18241 ttaccggtaa gcttgacgaa gatacccgtg tcattcccaa atcaattacc atgggaaagg
    18301 ttattaaggt ggtagagccc aagggatccc gctatgattt cagcttcatt gacaaatacg
    18361 ggtaccccaa tacagtggga ggcatttccc acatgttcaa ccaggcttac tggaattatg
    18421 ccaaactgat atcgggcgtt ttgcgtaacg gacttccggt agaagaagtg gtccacctgg
    18481 tatcttcgct ggaacttgac agccagacca tcaacaactg gcggacaggc gtggaaagag
    18541 cccttaagag atacatcccc aacggcacaa aggattcctc gggcacggaa tgtgaaaaat
    18601 gcggcgaacg gaacctggta taccaggaag gctgcctgtt gtgcatgagc tgcggttact
    18661 ccaaatgcag ttaaaggaat ttccatggcc acaaccgtag catatccttt ttccaaaata
    18721 aacatgggat tgcacgtgct tgaaaaaagg gcagacggct accacaacat tgaaacactt
    18781 ttctttccgc attcactaag cgatatcctg gaaattaccg atgttcccgg cgctccgcag
    18841 gatgtacagc tttttcttta cgggcttccg gttacagggc cttacgacgc caacctctgc
    18901 gttaaggctt accgcctgct tgcagagcgt tacaagctgc ccccggtaca tatacatctc
    18961 tacaagcaga ttcctccggg atcaggcctg ggtggcgggt cttctgacgc cgcagcctgt
    19021 ttgcagatac tgaacaagca ctatgatctg ggactgcgtc atgaggaatt gctatctcac
    19081 gccatacggc tgggcagtga tgtgcctttc ttcatccagt caacgccccc gctgccatat
    19141 ttcgcttccg gaaaagggga ggtgcttgag ccgttttccc ttgatttgag tgcttacagg
    19201 attgaattgc gtttcccgcc tgttgttatt tccaccgccg aggcttacac agccatcaaa
    19261 cctgacagta accgggtgcc attgcgggac ctgttgcggc aaccggttga tacatggagg
    19321 gatgctatca gaaatgattt tgaaacattc gtgtttcaga aatacccggg aaccattcaa
    19381 tacaagcagt ctctttatga agaaggagct atgtatgcct ccatgacagg aagcggttct
    19441 gctttttttg gacttttcaa aatctaggat tccggatttg ttcttatctt taccgtacat
    19501 atgggattgt attatcaaga gcaatttaag aacggggcgc agattgttgt ctgggaaata
    19561 acagaaaccg aggagcaatt acaggaaata tgtacccttc ccaatgaaga aagggaagaa
    19621 ctgtcttata tcaccaatcc gcagcgccgg ttggagcggc ttgccgtaag ggccatactc
    19681 catacccttt tccaggaaaa agtatacctg gggtatcatg aaaacggacg gccgtttcta
    19741 caaaactcta tcatagagct gagcatagcc catacccgcc gttttgcctg tgtcctgaca
    19801 cacccggaaa acaacgtggg aatagacatt gaattgcttg accgcaattt tacagcggtt
    19861 gaaaaaaaag ccatcgggga tgacgaaaaa aatttcctgt cagataaacc cgaggtccgc
    19921 aaattgcaac ttgctttgat atggtgcgcc aaagaggcct tgttcaaata catgtctcag
    19981 cccgatgtgg attatatcga gcaaatgaga attgacaaga tcgttccccg ggagacagga
    20041 gagatagatg cggtttttta tcagagggac ggtattgagg aagactttac cctggaatac
    20101 aaagtgctgg acgaccacgt catggtgtgg ctggttggtt gaacaacgcg gtgtactttt
    20161 ctacaaaaaa agcaggatga taactcattt tggcgatgcg caaccccatg tcacctgaat
    20221 cgtcctctcg gtttatatat tcaacatggg gatatttttc catcgcataa cggacaaaga
    20281 gctggttgat tgcctggtaa gcacccctga attcctggaa tgccttttcc acatgcacca
    20341 gccatgtgtt tttggtcagc agttccccca gggtataggc aacaatcttt ccgtctatca
    20401 ggatcatcaa accttccaga cccagttccc cgaatgagtt gaaagccctg tgcacggcac
    20461 aggtctcctg ttccagcccc acatcctggt tacagcccat gagccggcac cattcatcat
    20521 tcattgccag gcaggcaggg atatcttcca agcgtatctg tctggcttca aactccctgt
    20581 attcattcag gaaagagttc aggtgattcc ttttggcctg gaatttctta cctgtcaaaa
    20641 gagccaggtc ggaggcattg tagatataat cgaaggtatc cctgaaggat tgtacttcaa
    20701 cgtgggcagg ctcccgggcg gccagttgtc cggcttcttc tgcagaaagc acaccaacgc
    20761 tgaaaggtgc gtcttttgaa aaagcagaga ggatctccac ggctgttccc gcggcaacag
    20821 gaccatgcag gctcatggga tataaataat gaaacggttc tgccagattc ctgtggcttc
    20881 ttatcagcaa cagctcatct ccgagactgg agaaaacgct gaaatctgtg tggtaaatcc
    20941 ccgaccacat gaacaaggtg gcaaaactgt actcggaggc agctttcccg gctttggcca
    21001 ggtacggctt aatgatgccc tgatcaatca gggtaagggg tttgaatgag tccatattca
    21061 acaattattc attcagccag gattgcaatt gctccagcaa agcctctctc atggattgtg
    21121 gcagcgtacc tatgcgggca cggtgacttc ccccgggttg cacataatac aatacatttt
    21181 gttttccggg gtccggaacg gcagctgcag tccacggatc ccattcgccg tagatgaaaa
    21241 ccaggcgggc atcgctcttt tttacaaaat cacgttgcaa gcggtacata gatttattga
    21301 agaagggttt gtaaaaatcc ggcaaaaaaa gatctttcag atatcgttcc gcacttttca
    21361 gctccatggc aggatatttc ttggtaaccc attttccggc aacttgtttt ttcaccgtat
    21421 cctgttcagc ttccttttca ggtttttctt gtttgctgaa aaggcctttc tttgctttct
    21481 cgggaataaa acgtgtgcgg gttgttttga aacggaatgg cttggtgtca taaccgtaat
    21541 aacccagctc ccgggaagcc tgtatgaaga agggcagtgt gggagaatc
//
LOCUS       DAOSOF010000012        20973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2534_length_20973_cov_5.956736, whole genome shotgun sequence.
ACCESSION   DAOSOF010000012 DAOSOF010000000
VERSION     DAOSOF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_20973_cov_5.956736"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLM68_01405"
     CDS             complement(<1..770)
                     /locus_tag="PLM68_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT54061.1"
                     /translation="MRKIALPLAAALLVAVLHPFPAFAQEQKDTMPVPLGEIVVTSLR
                     LDRQIRNLPTSMVVVDSSLYQKRSAFTIANVLEEEPGVSRGGDGVWATNINVRGFSED
                     RLVILIDGNRVETATDLTASLSMIDVNDIERVEVIKGAQSSLYGTGAMGGIVNIITKQ
                     GHFGQKPYVTADVISGYASVNNLFSNHASVSAGSRKWYLNLGGTYAKAGNLRTPDGML
                     PNSRYNTSNISAKLGIRPLANHLFKIQYQRNWSWDVGVP"
     gene            complement(772..1017)
                     /locus_tag="PLM68_01410"
     CDS             complement(772..1017)
                     /locus_tag="PLM68_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54062.1"
                     /translation="MEKRIGTVIIQINSPKEVAHLNDILSLHAGIILGRQGLPRSNGK
                     NLISLILEGSTDQIGALTGQLGRLDGIQVKSVLLKNS"
     gene            1181..2140
                     /locus_tag="PLM68_01415"
     CDS             1181..2140
                     /locus_tag="PLM68_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT54063.1"
                     /translation="MRLPAIVCVCFLLLAGCGNASGKKDHAIPLTIAALKGPSSMGMI
                     RFIDSLEGVADAGLKVTIVNEPLQVRKMMLDGTADFALLPLTTAAIVYNKGLDYKLAA
                     IPVWGTLYLFGQDSAITKWEDLSHKKVHMMARGMTPDVLFRYLLQKNGIDPEHDITLD
                     YRFPTHIDLANAMASGQASLGVISEPLASQVMHRNDKVKMLFDLNQEWISIQGAPIPQ
                     TALMVKASVIRNNPAVVEEVLDAYERSVEWVNSHPDSAAVLIVKHLILDDPRIAYQAI
                     PRSGIKLVRAAAIRSQITEYLRIFYEMDPQTTGGKIPDEGFYY"
     gene            2151..2882
                     /locus_tag="PLM68_01420"
     CDS             2151..2882
                     /locus_tag="PLM68_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT54064.1"
                     /translation="MTVASIVLMLVIWKVLALAFNTPSIIPHPETTFLAVLKIFAEPG
                     FLAVAGSTVLRGVTGFVISGILGILTGILAGVSPGFNTFITPMLVSVRSIPVISLILL
                     ALIWFNAGAVPVFIAFLTMFPFICTNVSDGIRSVDPGLVEMARFYRVDKGRILTGVYI
                     PAIMPFIISGTSSAMGIGWRAIIIGEVLSQPQYGIGTRLQTAQSFLNVEAVIAWTLIS
                     VVISFIFEKLIRWGEHHIIRWQTGS"
     gene            2879..3583
                     /locus_tag="PLM68_01425"
     CDS             2879..3583
                     /locus_tag="PLM68_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54065.1"
                     /translation="MSLHIHNLSKSYKQPVFRDFSISFPEDTITCVLGPSGCGKTTLL
                     NIIGGIIPPDSGSLEGFENKTFSYVFQEPRLLPWKTVTENIEFVLKRELHTGERKKQA
                     VDLIRQVELHGFAGYYPSQLSGGMRQRVSIARAFAIPSDIILMDEPLKGLDAGLKKNL
                     INWFLKLWKTDRRTVIFVTHDVDEALLLSDRVIVLSQPPTRILTHLNIEEPTGERNPG
                     SERLLQLKQELLKNLF"
     gene            3700..5037
                     /locus_tag="PLM68_01430"
     CDS             3700..5037
                     /locus_tag="PLM68_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT54066.1"
                     /translation="MILQKRSVFVIITIVFVIAGYTAKAQTTPWTLERCIEYARQNNI
                     QIKSQKITKELSDFDLEQTRAQRFPTLNFSSNFGVSFQNTTTYNDFMEKTEATAYSDN
                     YGLNTGVTLYSGGKLNKTIKQKQIENSAYSYDIEQSALDIEISVIQAYLQILYANESL
                     EIAEKAVALSQTQVERGQEMYKAGSISKADLAQMQSQNASDKHQVTQAKNSLSSAKLS
                     LKQLLELELKENFDVVFTEFDDQQVLKIIPDLSSVYQSALNNLPQMKSSALSVESANT
                     AISVAKTSRLPTLSASASINTGTISSAGTSYFEQLGSKINENIGLSLSIPIFNNKQIR
                     TNINKAELQSQSAQLQDMSLKKQLLSTVESLHNDAVSAQSQYVSAKEQLDAAEASFEL
                     VNEQFLAGFKNTIELITEQNNYTNALSTLLQSRYQAVLSLMLLDLYQYQNVNY"
     gene            5048..6229
                     /locus_tag="PLM68_01435"
     CDS             5048..6229
                     /locus_tag="PLM68_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT54067.1"
                     /translation="MKKIFLLAQTWIMLTMLFSCNTKEMTPVYETTRAQIGSISNAVT
                     ATGTVEPIKQVEVGTQVSGIISKIYVDYNSEVKTGQLIAEVDRSVLETELESSKANLN
                     SSKAEYDYQTANYNRIKGLYDKELISQSEYDQAFNAYSKATYSYTQAKSNYQKAERNI
                     GYTWIYSPIDGVVLSRAVDEGQTVAAGFSTPTLFTIANDLKKMQVIADVDEADIGQVL
                     EGQHVVFSVDAFPEDQFEGTVIQVRLLATTTSNVVTYEVVIDAPNPDLKLKPGLTAEV
                     NIYTLNKENIMTVPVRALRFVPGEYTGNIPETHVFVQNPEGGIEAREVIVGVSDGIYT
                     EIIDGLSIEEKVMIGIQLSGSIKGVPGSSSNAPDDKENNGSNSPFMPPRPGQERNVRT
                     K"
     gene            6241..6981
                     /locus_tag="PLM68_01440"
     CDS             6241..6981
                     /locus_tag="PLM68_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT54068.1"
                     /translation="MSEIIKVENIKRDFLVGSETVHAIRDISFNVTEGEFVTIMGASG
                     SGKSTLLNILGCLDVPTQGDYYLSGVQVRKMGKNERAVLRNRKIGFVFQTYNLLPKTT
                     ALENVELPLFYNKTVNIAQRKEKSVQALHAVGLEDRMYHRSNQMSGGQQQRVAIARAL
                     VNDPVIILADEATGNLDTRTSFEILSVFQDLHKKGRTILFVTHNQEITQYGSRNIVLK
                     DGKIIEDKINHTISSAKDTLASLPVEED"
     gene            6993..8213
                     /locus_tag="PLM68_01445"
     CDS             6993..8213
                     /locus_tag="PLM68_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT54069.1"
                     /translation="MNYKNLITIALRALFNNKVRTFLTMLGIIIGVTSVITMLAIGSG
                     SKQSIRDEIGEMGSNMIMIQPGNGRFGGVRQSASDMQTLKMTDYESLLRETNYLSKIT
                     PTVNSSGQAVYRANNTPTTIYGVNEQYSDIRGYSVDEGSMFTSTDIQKAAKVCVVGQT
                     IVKNLFPDGENPLGKNIRFGKIPLKIIGILESKGYNSMGMDQDDILLAPYTTVQKRIL
                     AINYLQGITAAAISEEYSTQAIEEITEILQINHNIQIGQENDFNVRSQQEIMETMDSV
                     MDMMTILLGCIAGISLLVGGIGIMNIMYVSVTERTREIGLRMSIGAKGIHILMQFLIE
                     AVIISVTGGVLGVLLGVGASCLIPIITGWPVSVQAYSIILSFLVCTLTGVFFGWYPAQ
                     KASRLNPIDAIRYE"
     assembly_gap    8416..8515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8516..9435
                     /locus_tag="PLM68_01450"
     CDS             <8516..9435
                     /locus_tag="PLM68_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT54070.1"
                     /translation="LGYCGSITAYLLVFYINFFVFIKRFMLQKRWIVFFVSNLLLIST
                     VSILLYVWHGYYFTHLAGIPTEHIPPPQNSFVFILRDMFFMMLTASMAVAIRVTLEWQ
                     HTDREKAKMEIVASQAEIKNLKNQLNPHFLFNTLNNIYSLMRMDQNKAQDAVLSLSKT
                     LRYVLYDNNQEKVSLDKDLAFTKNYIDLMSLRLTDNVSMRVNISEKTEGLTIAPLMFI
                     TQVENAFKHGISQSAPSFIDINIDIQGKTISCSVKNSLFPKDVNDYSGSGIGIENLER
                     RLSLLYPGKHSYTYSAHDNEYLSELHITL"
     gene            9444..10157
                     /locus_tag="PLM68_01455"
     CDS             9444..10157
                     /locus_tag="PLM68_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT54071.1"
                     /translation="MTSLKCIIVDDEPLALNLLQDYIHQTPFLKLVARCSSGVEALDV
                     FNNEHVDLAFIDIQMPHLNGLELSRIIGNCFVIFTTAFEQYALEGFKVNAIDYLLKPF
                     SYAEFLTAANKAQRQAELVNASHSLGNSTLTPKSLVIKSDYKQQIINTSEIVYIEGIG
                     DYIKIHLETGISVQTLMTLKNIEEELPKNQFIRVHRSFIVNIQKVKTIERQRIVFDRV
                     RIPVSDFYKQAFFRALGIK"
     gene            complement(10181..11062)
                     /locus_tag="PLM68_01460"
     CDS             complement(10181..11062)
                     /locus_tag="PLM68_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT54072.1"
                     /translation="MKRLTLVLFLIVFGVEASAQTGWWGPQSKVVTDTLYSEVLNANR
                     EYTIFLPKSYETQTNREYPVLYLLHGMSGVNTSWFENEHAREIADQLIASGEACEMII
                     VSPNAGGNLAEGAWNGYFNMPGWPYETFFFTEFLPFIEKTYRIVGDKQHRAVAGLSMG
                     GGGATVYGQKHSDMFSSVYAMSALMDIPEGGGVPSQDPEDKMALLTKSVQENNCIKYV
                     EEASDERKDQLRSIKWFVDCGDDDFLLDRNIELTQAMRRQQIPCEFRVRDGGHTGEYW
                     RSALYICLPFVSRNFGK"
     gene            complement(11184..11257)
                     /locus_tag="PLM68_01465"
     tRNA            complement(11184..11257)
                     /locus_tag="PLM68_01465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11221..11223),aa:Pro,seq:cgg)
     gene            11311..12000
                     /locus_tag="PLM68_01470"
     CDS             11311..12000
                     /locus_tag="PLM68_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT54073.1"
                     /translation="MKRFLFLPVAIILVAGCHRTDPRNPYNLPIANTMKTYLEQVKEN
                     PDTELVDLEEVIPGIALHIAYATEENFTGVPIYTSAKAFLCHPVAMALAAAQDSLTKK
                     GLGFKILDAYRPYAGTLYFYKVYPDTTFVASPRTGSIHNRGAAVDLTLIRLDTGEELE
                     MPTLFDSFSDTASHTFTDLPDNVLENRALLRGIMEAYGFHSYEAEWWHYNYTNARSYP
                     LRDISFEELMK"
     assembly_gap    12207..12306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12827..13285)
                     /locus_tag="PLM68_01475"
     CDS             complement(12827..13285)
                     /locus_tag="PLM68_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOT54074.1"
                     /translation="MDFLLEWGYLGLFIGSFLASTIIPFSSEFLLIGMLLAGGNPWVC
                     LALSTIGNTLGSYTCYGLGYLGKWEWIEKWLRIKREKLEKQQQVILKWSSLVALLCWL
                     PVVGDVFSVGLGFYKIPFWKMAILMFIGKALRFLFWIGLWFLMPEKLLKL"
     gene            complement(13286..13444)
                     /locus_tag="PLM68_01480"
     CDS             complement(13286..13444)
                     /locus_tag="PLM68_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54075.1"
                     /translation="MKKSFVLRIDAETWAALEKWAADEFRSVNGQLEYIISEALRKNK
                     RLPEKNKK"
     gene            complement(13445..14329)
                     /locus_tag="PLM68_01485"
     CDS             complement(13445..14329)
                     /locus_tag="PLM68_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT54076.1"
                     /translation="MKNIQEKFIQPRNGWLMLFVSILGFMAGIALPIIFTESLGWAPV
                     WYFLLIVTVFSFIAMFGLMVINPNESKVITFFGKYVGTVVDNGFFMVNPFVVKKTISL
                     KARNLNSDPIKVNDKMGNPIMIGVVLVWRVKDTFKACFAVDNYEKFVDIQSEAAIRSL
                     AGSYPYDDLEDEHADITLRSGGEEVNDLLEVSLSERLNIAGIEIIEARISYLAYAPEI
                     ASAMLQRQQATAIVAARHKIVEGAVSMVEMALQQLSEKEIIELDEEKKAAMVSNLMVV
                     LTSDRAASPVINTGSLYQ"
     gene            complement(14515..15762)
                     /locus_tag="PLM68_01490"
     CDS             complement(14515..15762)
                     /locus_tag="PLM68_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOT54077.1"
                     /translation="MMKRLILSTMAVLLFACSNRNEQVPAADFVRIEGPYLFWQGERF
                     FIKGTNLGNWLNPEGYMFGFRKTNSAGWIDRMFRELAGPDFTAEFWKTFKDRYITQAD
                     IAFIASTGANTIRLPFHYKLFTDEDYMGLTAAQDGFDRIDSVVAWCRREGLHVILDMH
                     DAPGGQTGDNIDDSHGYPWLFESETSQQLFCDIWVKIADRYKNEPVILGYDLLNEPIA
                     PYFDNKDSLNTLLEPLYKRAVAEIRKVDTNHIVILGAPQWNSNFDPFTDWTFDNKIMF
                     SCHRYGGDATLEAITHFIDFRNKTALPMYMGEIGHNTLEWMEAFCKVMEDNNIGYTFW
                     PYKKIEGSCFVSIGTPEHWDQVVAFSEASRDTYAAIREARPDQELSRLAMQQFLELCL
                     FGNCSIEEDYIRALRLNEERTDN"
     gene            15823..17736
                     /locus_tag="PLM68_01495"
     CDS             15823..17736
                     /locus_tag="PLM68_01495"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOT54078.1"
                     /translation="MKNFQPPLHCLLLAATLLAAACDKTPDLPEEVKDPELKSISVEK
                     QQVVIPVQGAATILMQVDEPGFVFNYDITSPLCQVELRVQGTTNHPECYAIQSIAPAQ
                     SPGSYEVRLADKGIRMNYKEQAVLCLITSRAGYGKVMITTGAFEVISSLSKSGISAFS
                     FTKSVNPALDADVELKIASTETAYVNTIEGNIPCWLDDLKLIATFTSGNTVKVNEVVQ
                     ESGKTVNDFSVPVEYTVLTDEGTLLTYTVKVDLFTGLPVVIINTEGGRPVTSKEDWMN
                     ATIRIEGVDQFEDLPEMATEIRGRGNTTWFWPKKPYALKLESKTSLLGMPKHKRWVLL
                     ANFMDRTMLRNRIAFKIAQSTSLAWTPRNEIVELIFNGRHQGNYMLIEQIKEDKNRVN
                     IGDEGYILELDFHFDNVVQWISPHGQSVQQGGIPFAVKFPDEEEITPAQVDWIKNYID
                     QAGQAIYGPDFADPQTGYRKYLDMQSFVDYWLVYELCINHELANPGSVYMYKDRDTKL
                     FAGPTWDFDWGTFSYQASPQAKGILFMTEAIWYKQLFKDPEFRALAKERWNALKGDLE
                     QIPAFLDREYERLLPSAELNFKMWDPAESRNMNGGRLINGDEYLTYTQAVERMRGILV
                     ERLATLDEKINAL"
     gene            17752..20223
                     /locus_tag="PLM68_01500"
     CDS             17752..20223
                     /locus_tag="PLM68_01500"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOT54079.1"
                     /translation="MKRTQTILILAILAFSLQPLTAQRRPQEQNNTQSENKPDSKTES
                     KGPARLEEFIKAETAVMEGMTPVYVQDKKYFIGIPDSLIGRDILMVTRMSETPAGFRA
                     NFFGYAGDQINSGMLRFEKGPDNNIFLTKVLAREQSRDSTQAMYYNVMRSNRPAIIAS
                     FDIKALNADSTLSLIDVTDLVKGDNESLYFSKRQKKSSKMTTLQNDKSYVKSVRTFPI
                     NTELRSVKSYTHQDNDDPLTFEINCSLVLLPKEPMQPRYFDERVGYFTSNYTDFDMNP
                     QGIKTIRMIARWRLEPKPEDLEKYKRGELVEPAKPIIFYIDPSTPKEWVPYLIQGVND
                     WQPVFEKAGFKNAIYALEAPSPEEDPSWSLEDARNSAIVYKPSTIANASGPHVSDPRS
                     GEIIESHINWYHNVMSLVHNWYFVQCSPVDPLARSMTFPSELMGQLVRFVSSHEVGHT
                     LGLRHNFGATSYYTTEQLRDPEFLRANGHTASIMDYSRFNFVVQPEDNVSRELLFPRL
                     SHYDNWAIEWGYRRLYQFADVQEEIPYLNQWVIEKTKDPYLRFNGGSESGLNDPRAQS
                     EDLGDNQMETCELGIRNLKVIMQNLPEWTKVPNENYRGLSTLYPQITSQFSRYIGHVS
                     KWVAGVYVDAKTVEQEGPIYVNVSKNKQKEAMAFLERHIFTTPLWLLPDYLSELLPSS
                     RLSIMENLQNSAITGLVNENVLVRMLRAEEQLGPAAYSAEEFFKDMNRYVFGDYGQTD
                     IYCRSLQNIYVNTLCKMITPEKPSDSATTVAMMRRMSVSIENNDVKALVAAELETIAK
                     KLKRGRGDDRTRAHYNYLIKTIENL"
     gene            20223..20837
                     /locus_tag="PLM68_01505"
     CDS             20223..20837
                     /locus_tag="PLM68_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT54080.1"
                     /translation="MNYTHIVFDVDGTLLDTEKTGMVSLQQTVRQLMDREMTLEELYP
                     YFGIPSWEAVIRLGFEDPHHAAVVWEHHFQELLHLTVPFPGIEETLEVLHDNGIVMGV
                     VTSRNRAEFESDPYIARWKHFFTVTVCAEDSLAHKPAPDPMLTFISRTGAEPAGVLFV
                     GDTFYDQQCAHAAGTSFALATWGSRNEKDIPAEHYLNHPTDLII"
BASE COUNT         5771 a         4965 c         4709 g         5328 t
ORIGIN      
        1 ggaacgccta catcccatga ccagtttcgc tggtactgga ttttaaacaa atggttggca
       61 aggggcctga tgcccagttt tgccgagatg ttgctggtgt tataccggct gtttggcaac
      121 atgccgtcgg gagtgcgcag atttcctgcc ttagcatagg tgccacccag gttcagatac
      181 catttgcgtg aacctgcact gaccgatgcg tggtttgaaa acaaattgtt gacagaagca
      241 taccccgaga tgacatctgc cgtgacatag ggtttctgcc caaaatgccc ctgtttggta
      301 atgatattga ctatcccgcc catggctccg gttccgtaaa gggacgattg ggctcctttg
      361 atgacctcca cccgttctat atcattaacg tctatcatgc tgagcgatgc ggtcagatcc
      421 gtggccgtct ctacccggtt cccgtctatg aggataacca gccggtcttc actaaaaccg
      481 cgtacgttga tgttcgttgc ccagactccg tcccctcccc tggaaacacc cggctcttct
      541 tccagcacat tggctattgt gaaagcagag cgtttttgat ataacgagga atccaccaca
      601 accatggatg tcggcaggtt cctgatctgc ctgtccagac gcaggctggt caccacaatt
      661 tcgcccagcg ggaccggcat ggtgtctttc tgttcctgcg caaaggcagg gaatgggtgc
      721 aagacagcga ctaaaagggc tgctgctaaa ggaagtgcaa tttttctcat tttatgaatt
      781 cttcaataat accgacttga cctgaatacc gtccagcctt cccagctgac cggtcaatgc
      841 gccaatctga tcggtagatc cttcaagaat caaagaaatg agatttttgc cattacttcg
      901 cggaagaccc tgtcttccca ggataatgcc tgcatgaagc gaaaggatgt cgttcaaatg
      961 ggcgacttcc ttggggctgt tgatttgaat gatgactgtt cctattctct tttccatcag
     1021 aatacctccg aatcagattg gtttatgtgt tgatttacgt gccgtccttg aagaggaata
     1081 tcctgttctc agtacagaca cctgttttat tagcgacaca aagataagac attttttact
     1141 tctgcctaaa aatgtgctat ttttgttgaa actttgtata atgcggttgc cagccattgt
     1201 atgcgtttgt tttttactcc tggccggttg tggaaatgcg tccgggaaaa aagaccatgc
     1261 aatccctctc accatagctg ccctgaaagg gccttcttcc atgggcatga tccgttttat
     1321 cgacagtctg gaaggtgttg cagacgcagg cctcaaagtc actatcgtga acgagccgtt
     1381 gcaggtgagg aaaatgatgc tggacggcac tgccgacttt gccttgttgc ccctgacaac
     1441 cgctgccata gtatacaaca agggactcga ctataagctg gcagccatcc cggtctgggg
     1501 aacactctat ctctttggcc aggattcggc cataacgaaa tgggaagacc tcagtcacaa
     1561 gaaagtgcac atgatggccc gcgggatgac gcccgatgtg ctgttccgct acctgctgca
     1621 aaaaaacggg attgatccgg aacatgacat aacgctggat tacaggttcc ccacgcacat
     1681 agacctggcc aacgccatgg cctcgggaca ggcctccctg ggtgtgatct cggaacccct
     1741 ggcaagccag gtgatgcacc gcaatgacaa ggtaaagatg ctgttcgatc ttaaccagga
     1801 atggatatcc attcagggtg cacccatccc tcagacagct ctgatggtga aagcttctgt
     1861 tatccgaaac aatcccgctg tggtggaaga ggttctggat gcttatgaaa ggtctgtgga
     1921 atgggtaaac agccatcctg acagcgcggc ggtgctgatt gtcaaacacc tgatactgga
     1981 cgatccccgg atcgcgtatc aggccattcc caggtctgga attaagctgg taagggctgc
     2041 cgccatacgt tctcagataa cggaatatct tcggattttt tatgaaatgg acccacagac
     2101 aacaggagga aaaataccgg atgaaggctt ttattactaa aaaaaggatt ttgaccgttg
     2161 cttcaatcgt cctgatgctg gtgatctgga aagtgctggc actagctttc aacactcctt
     2221 ccattattcc ccaccctgaa accacctttc tggctgtttt aaagattttt gccgagccgg
     2281 gctttctggc cgttgcaggg agcaccgtgc tccggggagt aaccggcttt gtcatctcgg
     2341 gcatcctggg catcctgacc ggtattctgg caggggtcag cccgggattc aacaccttca
     2401 taacccccat gctggtctct gtacgttcca tccccgtcat ctcactcatc ctgcttgcgc
     2461 tgatctggtt caacgccgga gcggtcccgg ttttcatcgc tttcctgacc atgttcccgt
     2521 tcatctgcac caatgtttct gacggtatca gaagcgtaga tcccggactg gtggaaatgg
     2581 cccggttcta cagggtggac aaaggaagga tcctgaccgg ggtctatatt cctgccataa
     2641 tgccgttcat catttccggg acgtcaagcg ccatggggat cggctggcgc gccatcatca
     2701 ttggcgaagt cctgagccag ccgcagtacg gcataggaac caggttgcag accgcccaat
     2761 catttctgaa cgtcgaggca gttatcgcct ggacattgat ttccgtggtg atcagtttca
     2821 tatttgaaaa actgatccgc tggggagaac atcatatcat tcgctggcaa accggatcat
     2881 gagcctgcac atacataatc tgagcaagag ttacaaacag cctgtcttca gggatttttc
     2941 catttcattt cccgaagaca ccataacctg cgtcctgggg ccttctggat gcggaaaaac
     3001 caccctgctc aacatcattg gcggtatcat cccgccggat agcggctcgc tggaaggatt
     3061 tgaaaacaaa acgttttcct acgtcttcca ggaaccccgc ctgctgccct ggaaaacggt
     3121 caccgaaaac attgagtttg tactcaaacg ggaattgcat accggagagc ggaaaaaaca
     3181 ggccgtagac ctgatccggc aagtggagct gcacggtttt gccggttatt atccctccca
     3241 gctgagcgga ggcatgcgcc aaagggtgtc cattgcccgg gcctttgcca ttccctcgga
     3301 catcatcctg atggacgaac ccttaaaggg actcgatgcg ggattaaaaa agaacctgat
     3361 caattggttt ctgaaactct ggaaaaccga ccgtcgcact gtgattttcg tgactcacga
     3421 cgtggatgaa gcacttctgc tgagtgaccg ggtgattgtg ctaagccagc ctcctacccg
     3481 gatcctgact catctgaata ttgaagaacc gaccggggaa cgcaaccccg gctctgaacg
     3541 gttgttgcaa ctcaagcagg aattgttaaa aaatcttttt tgactcggaa aaaggcccca
     3601 aaaaagtcta tctgacatga aaatacggcg ttctgccgat tttgtttgaa ggttgtattc
     3661 ttcccggata catttgccat caataaaaaa tgttcgatta tgattctaca aaaaagatcc
     3721 gtatttgtaa ttataaccat cgtgtttgtt atagcaggtt atacggcaaa agctcaaacg
     3781 acaccatgga ccctggaaag gtgtattgaa tacgccaggc agaataatat ccagatcaaa
     3841 agtcagaaaa taacaaaaga actatccgac tttgacctgg aacaaaccag ggcacagcgt
     3901 tttcctactc ttaatttttc atcaaacttc ggggtttctt ttcaaaacac gaccacatac
     3961 aacgacttca tggaaaaaac agaggctacg gcctattcag ataattatgg gctcaatacc
     4021 ggtgtaacat tatactccgg tggcaaactg aataaaacca taaaacaaaa acaaattgaa
     4081 aattcagcgt attcatacga catcgaacaa tcggcactgg atatagaaat atcagtcatt
     4141 caggcctatc tacagatttt gtatgccaat gaatcattgg aaatcgcaga aaaagccgta
     4201 gctctttcac aaacacaggt tgaaagaggg caagaaatgt ataaggccgg atccatatcc
     4261 aaagccgatc tggctcaaat gcaatcacaa aatgccagtg acaaacatca ggtaacccag
     4321 gctaaaaact ctttatcaag tgctaaactg tcattaaaac aattgctaga gctggaactg
     4381 aaagaaaatt ttgacgtcgt ttttacggag tttgacgatc agcaagtcct gaaaattatt
     4441 ccggatttgt cttccgtata tcagtctgca ttaaacaatt tgcctcaaat gaaaagtagt
     4501 gccttaagtg tggaatcggc aaatacggcc atatctgttg ccaagacttc caggttgcct
     4561 accctatctg ccagcgcctc tatcaatacc gggactatct caagcgcagg aacctcttat
     4621 tttgaacaac ttggcagcaa gatcaatgaa aacatcgggc tcagtctaag catccccata
     4681 ttcaacaaca aacaaattcg caccaatatt aataaggctg aattgcaaag tcaatccgct
     4741 caattacagg atatgagttt aaaaaaacaa cttttaagca ccgttgaatc cctccataac
     4801 gatgctgttt ctgcgcaaag ccagtatgtt tctgcaaaag aacaactgga cgcagccgaa
     4861 gccagttttg aacttgtaaa tgaacagttt cttgccggat ttaaaaatac catcgaactg
     4921 atcacagaac aaaataatta tacaaatgcg ctcagcacct tgcttcaatc aaggtatcag
     4981 gctgttttat ctcttatgct gctggatctg taccaatatc aaaatgtgaa ctattaataa
     5041 catcaaaatg aagaaaatat ttttattagc ccagacatgg atcatgctga caatgctgtt
     5101 ctcctgtaat acaaaggaaa tgactcctgt gtatgaaacg acccgggcac aaattggctc
     5161 aataagtaat gccgtaacgg ctaccgggac agtggagccc ataaaacagg tagaggtagg
     5221 gacgcaggtt tcggggatta tttccaaaat atacgtggat tataattctg aagtcaaaac
     5281 cgggcaactt attgccgagg ttgaccgatc agtccttgaa accgaactgg aatcctctaa
     5341 agccaattta aattcttcca aagcagaata cgattatcag acagccaatt acaatcgtat
     5401 aaaagggttg tacgataaag aactgataag ccaatctgaa tacgaccagg cattcaacgc
     5461 ttattcaaag gctacttact cctataccca ggccaaatcc aattatcaaa aagccgaaag
     5521 aaacatagga tacacctgga tctattctcc tattgacggc gttgttctct cgcgtgcggt
     5581 tgatgaaggt cagacggttg cagccggttt tagtactcca actcttttca ctatagccaa
     5641 tgacctgaaa aaaatgcagg ttattgccga tgttgatgaa gccgatatag gacaagtatt
     5701 ggaaggccaa catgttgtgt tttccgtaga tgcttttccc gaagatcagt ttgaagggac
     5761 ggtcattcag gtacgcctgc tggctacaac tacttccaat gtggttactt acgaagtggt
     5821 aattgatgct cctaatcctg atctgaaact aaaacctggc cttaccgctg aagtcaacat
     5881 ctataccctc aacaaagaaa atatcatgac cgtgcccgtg cgtgccctcc gtttcgttcc
     5941 gggggaatac accggaaaca tcccggaaac acatgtcttt gttcaaaatc ctgaaggagg
     6001 tattgaagcc cgggaagtca ttgtcggtgt ttccgacggg atctatacag aaattattga
     6061 cggtctgtca atagaagaaa aagttatgat agggatacaa ctctcaggca gtataaaagg
     6121 agtacccggc tcttccagca acgcccctga tgataaggaa aataacggaa gcaatagtcc
     6181 gttcatgcct ccccggccgg gacaggaaag aaatgtacgt acaaaataag gaagaatcta
     6241 atgagtgaaa ttataaaagt agaaaacatc aaacgcgatt ttttggtggg cagcgaaacg
     6301 gttcatgcca taagagatat ttctttcaat gttacagaag gtgaattcgt gactataatg
     6361 ggagccagcg gatcagggaa atctactttg ttaaatatat tgggttgcct tgacgtacct
     6421 acacagggtg actattacct ttcaggtgtt caggtgagaa aaatggggaa aaacgaaagg
     6481 gctgttttac gcaaccgtaa aattggattt gtatttcaaa cctataacct gttgccaaag
     6541 acaacggcac tggaaaacgt ggagttacct ttattttata ataaaaccgt caacattgcg
     6601 caaagaaagg aaaaatctgt tcaggcctta catgccgtag ggcttgaaga ccggatgtat
     6661 catcgttcca accagatgtc gggaggacaa caacaacggg tggcaattgc ccgtgctctt
     6721 gtaaacgacc cggtcattat cctggccgat gaagctacgg gaaatctgga taccaggaca
     6781 tcatttgaaa ttctatctgt tttccaggat ttgcataaaa aaggccgtac tattcttttt
     6841 gtcacacaca atcaggaaat cacacaatac ggaagtcgca atattgtgtt gaaagatgga
     6901 aaaattattg aagacaagat caatcacact atttcatctg ccaaagacac tttggcttca
     6961 ttacccgttg aagaagatta aaagactgca caatgaatta taagaatctg ataacaatag
     7021 ctttaagggc tctgtttaat aataaagtga ggactttcct gactatgctg ggaatcatca
     7081 taggagtgac ttctgttatt actatgctgg ccatcggatc aggctccaaa caaagtatcc
     7141 gggacgaaat aggtgaaatg ggatccaaca tgatcatgat ccagcccggg aatggccgtt
     7201 ttggaggagt tcgacaaagt gcttccgata tgcagacact taaaatgacg gattacgaat
     7261 cattattacg tgaaaccaat tacctgtcca agattactcc cactgtcaac agtagcggac
     7321 aggcagtgta cagggccaat aatacgccta ccactattta tggagtgaat gaacagtaca
     7381 gtgatatacg cgggtactcg gtagatgaag gctctatgtt tacctctaca gacatacaaa
     7441 aagccgcgaa agtctgtgtc gtgggacaaa ccattgtgaa aaacctcttt cccgacggag
     7501 aaaacccttt gggcaaaaac atccgttttg ggaagattcc actaaagatt ataggcattc
     7561 ttgaatcaaa aggatataat tcaatgggga tggatcagga tgatatcttg ctggctccct
     7621 ataccacggt tcaaaaaaga attctggcca taaattattt acagggtatt accgccgcag
     7681 ctatttctga agaatactcc acccaggcca ttgaagaaat tactgagata cttcaaataa
     7741 accataatat tcaaattgga caggaaaacg attttaatgt acgttctcaa caggaaataa
     7801 tggaaaccat ggattcggta atggatatga tgaccattct tttaggttgt atagccggca
     7861 tctctctgtt agtaggcgga atcgggatca tgaatattat gtatgtttcg gtaactgaac
     7921 gcacccggga aataggatta cgtatgtcca tcggagccaa aggcatacat atacttatgc
     7981 aatttttgat agaagccgtg attataagtg ttaccggagg tgttctgggt gtacttttag
     8041 gcgtgggtgc atcgtgtttg atacctatca ttaccggatg gcctgtttct gtacaagcat
     8101 attccattat tctttccttc ctggtctgta ctttaaccgg tgtgtttttt ggttggtatc
     8161 cggcgcaaaa agcttccaga cttaatccta tagatgcaat caggtacgaa taaagaattt
     8221 attatctttg aaaatgacta tgatatcgga aaaaaaaagc agacgggtgt ttattctcat
     8281 gaatattatg gccgtggtgt tgttttatag tcttcccatt tttataaatc cacgcaatga
     8341 gacactcact ttcgtgaggt acctgggata ttgtggcagc atcactgctt atttgctggt
     8401 tttttacatt aatttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacctg
     8521 ggatattgtg gcagcatcac tgcttatttg ctggtttttt acattaattt ttttgtattc
     8581 ataaaacgat ttatgctgca aaaacgatgg atcgtttttt tcgtgtctaa tctattatta
     8641 ataagcacgg taagcatcct gctttatgtg tggcatgggt attattttac ccatttggca
     8701 ggcatcccga ccgaacacat tccgcctcct caaaattctt ttgtttttat tttaagagat
     8761 atgtttttta tgatgcttac tgcttccatg gccgtagcta tacgcgtaac tcttgaatgg
     8821 cagcacactg acagagaaaa agcaaaaatg gaaattgttg ccagccaggc agaaattaag
     8881 aatctaaaaa accagctcaa tccgcacttt ctgttcaata cattgaataa catctattct
     8941 ttaatgagaa tggatcagaa taaagcccaa gatgccgttt taagtctcag caagacattg
     9001 cgttacgtgt tgtatgacaa caatcaggaa aaagtgtctt tagataaaga tctggctttc
     9061 acaaaaaatt acattgatct tatgtccctg cgccttactg acaacgtcag tatgagagtc
     9121 aacatttcgg aaaaaacaga agggttgact attgcaccgt taatgttcat tacacaggta
     9181 gaaaacgctt tcaaacacgg tatttctcag tcggcccctt catttattga tatcaatatt
     9241 gatattcagg ggaaaacaat ctcctgctcg gtaaaaaaca gtctattccc aaaagatgtc
     9301 aatgattaca gtggttccgg aattggcatt gaaaatttag agcgacgcct ttctttatta
     9361 taccctggaa aacattctta tacttattcc gcacatgata acgaatatct ttctgaactg
     9421 catattacct tatgatttga ataatgacaa gtttaaaatg cataattgtt gatgacgaac
     9481 cgttggcatt gaatctgctg caggattaca ttcaccagac cccttttctt aagctggtgg
     9541 ccaggtgcag cagtggtgtg gaagcattgg atgtgtttaa taatgaacat gtagatctcg
     9601 ctttcattga catacaaatg ccccatttga acgggctgga attatcccgg ataattggaa
     9661 attgttttgt catattcacc acggcatttg aacaatatgc attggaagga tttaaagtga
     9721 atgccatcga ttacctgctt aagcctttca gttatgcaga atttctgaca gcggccaaca
     9781 aggcccaacg ccaggcagag cttgtgaacg cttctcattc actgggaaac agcacgttga
     9841 ctccaaaaag tcttgttatt aaatccgact acaaacaaca aattatcaat acatcggaga
     9901 ttgtttatat agaagggatt ggcgattata taaaaatcca cctggaaacc ggtatttccg
     9961 ttcaaactct tatgacactg aaaaacattg aagaagaatt accgaaaaac cagtttatac
    10021 gagttcaccg ttctttcatt gtgaacattc aaaaagtcaa aaccattgaa agacaacgga
    10081 ttgtctttga ccgggtaaga atcccggttt ctgactttta taaacaagct ttttttcggg
    10141 ctttggggat aaaataaaac ccgggttttt actccgctat ttacttccca aaatttcttg
    10201 acacaaaagg caaacagata taaagtgccg aacgccagta ttcaccggtg tgcccgccat
    10261 cgcgcacccg gaattcacag ggaatttgct gacggcgcat agcctgcgtt aattcaatat
    10321 tacggtcaag cagaaaatca tcatcaccac aatcaacaaa ccattttatg ctgcgaagct
    10381 ggtctttacg ttcatcactc gcttcttcaa catactttat acaattgttt tcctgtacgg
    10441 attttgtaag taatgccatt ttatcttcag gatcctgcga aggaacccct cctccctccg
    10501 gaatgtccat taaggcactc atggcataaa cagaagaaaa catgtcactg tgtttttggc
    10561 cgtatacagt ggccccgccg ccacccattg ataaaccggc aacggcacgg tgctgtttgt
    10621 ctcctacaat acgatatgtt ttttcaataa agggcaggaa ttcagtaaag aaaaatgtct
    10681 cgtatggcca accgggcata ttaaagtagc cgttccacgc accttcggcc aaattgcctc
    10741 ccgcgttagg cgatacgatg atcatctcac aagcctcgcc cgaagctatc aattggtcgg
    10801 ctatctccct ggcatgttca ttttcaaacc agctggtatt gacacccgac ataccatgta
    10861 gaagatagag aacgggatat tccctattcg tctgggtttc gtaacttttg ggaaggaata
    10921 ttgtatattc acggttggca tttaaaacct cactgtaaag ggtatctgtt accaccttac
    10981 tttgtggccc ccaccatcct gtttgtgctg atgcttcaac accaaataca ataaggaata
    11041 atactaatgt aagacgtttc atataatgtt aaattaagtg atacaatctc ttctatacat
    11101 cacatgactt cctgaatgac aacggaattg ctaagaaggg taaaatcggg caaattataa
    11161 gcatttaact gcatgaatgc ttgtcggggt gaaaggactc gaaccttcgg cctccacgtc
    11221 ccgaacgtgg cgcgctagcc aactgcgcta caccccgaat gatcaagggt gcaaaggtag
    11281 ataaaatatt taattcctta tctttgattt atgaaacgct ttttatttct gcctgtagcc
    11341 ataatactag ttgccggttg tcaccggaca gatccgcgaa atccttataa cctgcccatt
    11401 gccaatacaa tgaaaacata cctggaacag gtaaaagaaa acccggacac cgaattggtg
    11461 gatctggaag aagtcatccc cgggatcgct ttgcatatcg cttatgctac ggaggaaaat
    11521 tttacaggcg tacccatcta tacctctgct aaagctttcc tttgccaccc ggtagccatg
    11581 gcactggcgg cagctcagga ttctctcaca aaaaaaggcc tgggattcaa gatcctggat
    11641 gcctaccgtc cctatgccgg caccctatat ttttacaagg tatatcccga taccacattt
    11701 gtagccagtc cccgcaccgg ttccattcat aaccgcggag cggccgtaga cctgacactt
    11761 atacggctgg atacaggaga agaactggaa atgcccacac tgttcgattc attttcagac
    11821 actgcatccc acactttcac tgaccttccg gataatgttc tggaaaacag ggccctgtta
    11881 cggggcatca tggaagccta tgggttccac agttacgaag cggaatggtg gcattacaat
    11941 tataccaatg cacgttccta tccgctgcgg gacatttcct ttgaagaatt gatgaaatag
    12001 gaagaggtgg aacctcatgc gagccgtcac ctgttagctg tataaggtcg cttcacttca
    12061 agaccagggg agtttttggg gaacgccgag gcttccatct tttctgcagc aggatgcctg
    12121 actgttaaga acagagcgct ttttcctgaa agccgaggct tccatctttt ctgcagcagg
    12181 atgcctgact gttaagaaca gagcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnncatc ttttctgcag caggatgcct gactgttaag aacagagcgc tttttcctga
    12361 acgccgaggc ttccatcttt tctgcagcag gatgcctgac tgttaagaac agagcgcttt
    12421 ttcctgaaag ccgaggcgct cttttcttaa cagtcaggca tccttgcagt tatgcgtata
    12481 gctgcctcaa caacttccgg tggtcttcga tgcatagcac caccaagtgc cccgcaaaca
    12541 ttgttctttt agttgatatt caagatgtta tgtaattcat acccccgggg gtatggaccg
    12601 cataagtagc tgattatgag gaaaacgaac caagtttgcg gggcactgct ggtgggtggt
    12661 cgctaacgca acaagataag ggaagcctcg gaataagaaa aaagcttccc gttcttgttg
    12721 cgttagcgac cgcagcagtc aattaagtaa cagagagcct cggcgtaaca aaaaagctct
    12781 cagtcccatg tgatgtcagc gactgcatgc taataaaagg agagggtcag agcttcagca
    12841 atttttcagg catcaggaac cagagtccga tccagaagag gaacctgagg gccttgccaa
    12901 tgaacatgag aatggccatt ttccagaacg ggatcttgta aaagcccaat ccaaccgaaa
    12961 agacatctcc gactaccggc agccagcaca atagggccac caggctgctc catttgagga
    13021 tcacctgttg ctgtttttcc agtttttccc tttttatgcg cagccatttt tcaatccatt
    13081 cccatttacc caggtacccc agaccataac aggtatagct ccccagggtg ttgccgatgg
    13141 ttgacagggc aaggcagacc catggattgc ctccggccag aagcatgcct atcaagagaa
    13201 actcggagct gaagggaatg atggtcgagg ccaggaaaga ccctatgaac aatccaaggt
    13261 agccccattc cagtaggaag tccatttatt ttttgttttt ctccggtaat cttttgtttt
    13321 tcctcaacgc ttcgcttatg atgtattcaa gctggccgtt aacgctacgg aactcgtctg
    13381 ccgcccattt ttcaagcgca gcccatgtct ctgcgtcaat gcgtaaaaca aacgattttt
    13441 tcatttactg gtaaaggctt cctgtattga tgaccggact ggccgcacgg tcagaagtca
    13501 ggacaaccat caggttgctg accatggccg ctttcttttc ttcgtccagt tctatgattt
    13561 ctttttccga taattgctgc agggccattt ccaccatact gacggcccct tccacgatct
    13621 tgtggcgagc ggcgacaatg gccgttgcct gctggcgttg cagcatggcg ctggcaatct
    13681 cgggcgcata ggccaggtaa ctgatccttg cctctatgat ttcaatgccg gcgatattca
    13741 atctttcgga gagagacacc tccagcaggt cattcacctc ttcccctccg gaacgaaggg
    13801 ttatatcggc atgctcatcc tcaaggtcat cataaggata ggacccggcc aggctcctga
    13861 tggctgcctc tgactgtatg tccacgaact tttcataatt gtccacggca aaacaggcct
    13921 tgaaggtgtc ttttacccgc cagaccaaaa ccacccctat catgatggga ttacccatct
    13981 tgtcgtttac cttgatgggg tcgctgttca ggttgcgagc cttcagcgat atggttttct
    14041 tgaccacgaa tggattgacc atgaagaatc cgttatccac cacggttccc acatattttc
    14101 caaaaaaagt gatcaccttg ctttcgttcg gattgatgac cataagacca aacatggcta
    14161 taaaagaaaa caccgttacg atcagcagaa aataccagac cggggcccac cccagcgatt
    14221 ctgtaaaaat tatgggcagc gctatgcctg ccataaaacc gagaatagac acaaaaagca
    14281 tcaaccagcc attccttggt tgaatgaatt tttcctgaat gtttttcatc tttgtcaata
    14341 tttgtttttt tgatatcttt ttgatatcac aaagatatta aaatttcaga aatggacaaa
    14401 aaaatattct tgtccatgga aaaagtttgg cacgctgttt gcaatttatt agggcagata
    14461 gttttttttc atctattagg ttttaggtta aatgggtcag gacaggcaac gcttctagtt
    14521 gtctgtcctt tcttcattca ggcggagtgc ccttatgtaa tcttcctcga tactgcaatt
    14581 cccgaacagg caaagctcca ggaactgctg catggcaagg cggctcagct cctggtccgg
    14641 cctggcttcc cttatggctg catacgtatc cctggaggct tcggaaaagg caaccacctg
    14701 gtcccagtgt tcaggtgtac ctatggagac aaagcaggaa ccctcaattt ttttatacgg
    14761 ccagaaagtg tacccaatat tattatcctc catgactttg caaaaagcct ccatccactc
    14821 cagggtgttg tgcccgattt ctcccatgta catgggcaag gctgtcttgt tcctgaaatc
    14881 aatgaaatgc gtaatggctt ccagtgtggc atcaccgcca taacggtggc aggaaaacat
    14941 gatcttgtta tcgaatgtcc agtcggtgaa cgggtcaaag ttgctgttcc attgaggagc
    15001 cccaaggatg acaatgtgat tggtgtctac cttgcggatt tcggccactg cccttttgta
    15061 taagggttca agcagggtgt tcagggaatc cttgttgtcg aagtacgggg caatgggctc
    15121 gttcagcagg tcataaccca ggatgacggg ctcgttctta tacctgtcgg cgatttttac
    15181 ccagatatca caaaaaagct gctggctggt ctcactttca aaaagccacg ggtacccgtg
    15241 actgtcgtca atgttgtccc cggtctgccc tcccggggca tcgtgcatat ccaggatcac
    15301 atgcaggcct tcccgccggc accaggccac aacgctgtca atgcggtcaa aaccgtcctg
    15361 cgctgccgtc agtcccatgt agtcctcatc tgtaaacaac ttgtaatgaa agggcaaacg
    15421 aatggtattg gccccggtgg aagctatgaa ggcaatgtct gcctgcgtaa tatacctgtc
    15481 cttgaatgtt ttccagaatt ctgccgtgaa atccggacct gccagttcac ggaacatcct
    15541 gtcaatccag cctgcagagt ttgtttttct gaaaccgaac atatacccct ccggattgag
    15601 ccagtttccc aggttggtac ctttgataaa aaaacgttct ccttgccaaa agagataagg
    15661 tccttcaatc ctgacaaaat ctgccgcggg aacttgttca ttgcggttgc tgcaggcaaa
    15721 aagcaacacc gccattgttg aaagaataag tcttttcatc ataaatgctg ttgttttatc
    15781 ttatggtaaa ggtaaaattt tataacttgc aagccaaagt acatgaagaa ttttcaacca
    15841 ccactccatt gcctgttatt ggctgccacc cttctggcag ctgcctgtga caagacaccc
    15901 gatctgccgg aagaagtaaa agaccctgaa cttaaaagca tatctgttga aaaacaacag
    15961 gttgttatcc ctgtgcaagg agccgccacc atcctgatgc aggtagatga accgggtttt
    16021 gtcttcaatt acgacatcac atccccgcta tgccaggttg aattgcgggt ccagggcacg
    16081 acaaaccatc ccgaatgcta tgccattcaa agtattgctc cggcgcaaag ccccggttcc
    16141 tatgaggtcc gccttgccga taagggtatc cgtatgaatt acaaggaaca ggcggtcctg
    16201 tgcctgatca cctcccgggc gggctatgga aaggtcatga tcacaaccgg agccttcgaa
    16261 gtaataagct ccctgagtaa atcgggaatc tcggcctttt cctttacaaa atcggtgaat
    16321 cctgcccttg acgcggacgt ggagctgaag attgcttcaa cagaaacagc ttatgtgaac
    16381 accatagaag gaaatattcc atgctggctg gatgacctga agctgattgc gacctttaca
    16441 agcggcaata cggtgaaagt gaacgaggtg gtgcaggaaa gcgggaagac ggtcaatgat
    16501 ttttcagtac cggtggagta taccgtcctg accgatgaag gcaccctgct gacctatacg
    16561 gtgaaagtgg atctttttac cggtctgcct gtggtgatca taaatacaga ggggggaaga
    16621 ccggttactt caaaagagga ctggatgaat gccaccattc gtattgaagg tgtggatcag
    16681 tttgaagacc ttcctgaaat ggcaactgaa atccgtggac ggggaaacac aacctggttc
    16741 tggcccaaga aaccttatgc cctgaaactg gagagcaaaa catcgctcct gggcatgccc
    16801 aagcacaaac ggtgggtgtt gctggccaat ttcatggacc gcaccatgct gcgcaaccgc
    16861 atagctttca aaattgcaca atccacctcc ctggcctgga ctccccgcaa cgaaattgtg
    16921 gaactcatat tcaacggtcg ccaccagggc aattacatgc tcattgaaca gatcaaggaa
    16981 gacaagaacc gtgtgaatat aggtgatgaa ggatatatcc tggagctgga ttttcatttt
    17041 gacaatgtgg ttcagtggat ttcaccgcac ggacagagcg tccaacaggg cgggatccct
    17101 tttgccgtca aattccccga tgaagaagag attactcctg cacaggttga ttggataaaa
    17161 aattacatag atcaggccgg gcaggccatc tacgggccgg actttgccga tccgcagacc
    17221 ggttaccgga aatacctgga catgcagtct tttgtggatt actggcttgt ctatgaattg
    17281 tgcatcaacc acgaactggc caatccgggc agtgtctata tgtataaaga cagggacacc
    17341 aaactgtttg caggtcccac ctgggatttt gactggggaa cgttttccta ccaggcttct
    17401 ccgcaggcaa agggaatact cttcatgaca gaagccatct ggtacaaaca gcttttcaaa
    17461 gaccctgaat tccgcgccct ggcaaaagag aggtggaatg cgctgaaagg ggatcttgaa
    17521 cagatccctg cgttcctgga ccgtgaatac gaacgtctgt tgccttctgc cgaattgaac
    17581 ttcaagatgt gggaccctgc cgaatcgagg aatatgaacg gaggcaggct tatcaacgga
    17641 gacgaatacc tgacttacac ccaggcggtg gaacggatgc ggggcatcct ggttgaacgc
    17701 ctggcaacac tggacgaaaa aatcaatgct ttataaataa accgttacac aatgaaacgc
    17761 actcaaacca ttctaattct ggccatcctg gccttttccc tgcaaccctt gacggcacag
    17821 cgccggccgc aggaacagaa caacacccag tctgaaaaca aacccgactc aaagacagag
    17881 tccaaaggac cagccaggct ggaagaattc atcaaggcag aaactgcggt aatggaggga
    17941 atgacacccg tttatgttca ggacaagaag tacttcatcg ggatccccga ctcgctcata
    18001 ggcagggata tccttatggt gacccgcatg tcagaaacac ctgccggatt ccgtgccaat
    18061 ttcttcggat atgccggtga ccagataaac agcggcatgt tgcgctttga aaagggaccc
    18121 gacaacaata tcttcctgac caaagtgctt gcacgggagc aatccaggga cagcacccag
    18181 gcaatgtatt ataatgtgat gcgttccaac aggccggcca tcatcgcttc ctttgacatc
    18241 aaggccctga atgcagacag cacgctttcc ctaatagatg tgacagacct ggtgaaaggc
    18301 gacaatgaga gcctgtactt cagcaaaagg cagaagaaat cctccaagat gaccaccctg
    18361 cagaatgata aatcctatgt caagagtgtc aggactttcc ccatcaacac cgaattgcgc
    18421 agtgtcaagt catataccca tcaggacaac gatgatccgc tgacattcga gatcaactgt
    18481 tcgctggtgc ttttgcccaa agaacccatg cagccccggt attttgatga acgggtgggt
    18541 tatttcactt ccaattatac cgattttgat atgaatccgc aggggatcaa aaccatacgc
    18601 atgatagccc gctggcgcct ggagccgaaa ccggaagacc tggaaaaata caaaaggggc
    18661 gaactggtgg agcctgcaaa acctatcata ttttacatcg atccctcaac cccgaaggaa
    18721 tgggttccct atctgatcca gggcgtgaac gattggcagc ccgtatttga aaaagccggt
    18781 ttcaagaatg ccatttatgc gctggaagcc ccctctccgg aagaagatcc ttcctggagc
    18841 ctggaagatg cccgcaactc ggccattgtc tataaaccct ccaccatagc caacgccagc
    18901 ggtccccacg tgagcgaccc ccgttcgggc gagatcattg aaagtcacat caactggtat
    18961 cacaacgtga tgagcctggt tcacaactgg tattttgtac agtgttcccc cgtagatccc
    19021 ctggcacgct ccatgacctt cccctctgaa ttgatgggac agcttgtccg gtttgtttcc
    19081 tctcatgagg tgggacatac cctgggattg cgtcacaatt ttggggcaac ctcgtattat
    19141 acgacggaac agctcaggga tcccgaattc ctgcgggcca acggacatac cgcatccatc
    19201 atggactatt cccggtttaa ctttgtggtg caaccggaag acaatgtctc ccgtgagctg
    19261 ctgtttcccc gtttaagcca ttacgacaac tgggccattg aatggggcta caggcgtctt
    19321 taccagtttg ccgatgtgca ggaagaaata ccttatttga accagtgggt cattgaaaag
    19381 acaaaagatc cgtacctgag gttcaacggt ggctcggaat cgggcctgaa cgatccgcgt
    19441 gcccaatctg aagacctggg agacaaccag atggaaacat gcgagctcgg aatcaggaat
    19501 ttgaaagtca ttatgcaaaa cctgcccgaa tggaccaagg tccccaatga aaattacagg
    19561 ggactctcca cattataccc ccagataaca tcccagttca gccgctacat cggccatgtt
    19621 tccaaatggg tggcaggggt gtacgtcgat gccaaaaccg tggaacagga aggtcccata
    19681 tatgtgaatg tttccaaaaa caagcaaaaa gaggccatgg cattcctgga acgccatatt
    19741 ttcacaaccc cgctgtggct gcttccggat tacctctcgg aactgcttcc ctccagccgg
    19801 ttgtccatca tggaaaacct ccagaatagc gccataacgg gactggtcaa tgaaaatgtc
    19861 ctggtacgca tgcttagggc ggaagaacag ctgggacccg ccgcctacag cgcggaagag
    19921 tttttcaagg acatgaacag atatgtattt ggcgattacg gtcagacaga catctattgc
    19981 aggagcctgc agaacatcta tgtcaatacg ctgtgcaaga tgataacacc ggaaaagcca
    20041 tcagacagcg ccacaacggt ggccatgatg cgcagaatga gtgtttccat agagaataac
    20101 gatgtgaaag cccttgtggc agcggaactg gaaaccatcg ccaaaaaact caaacgcggc
    20161 cgaggagacg accgcacccg cgcccattac aattacctga tcaaaaccat agaaaacctg
    20221 tagtgaacta cacacatatt gtatttgacg ttgacggcac ccttctggac actgaaaaga
    20281 cagggatggt atccctgcag cagacagtac ggcagctgat ggaccgcgaa atgacgctgg
    20341 aggaactgta cccgtatttt ggaataccca gctgggaggc cgtcatccgt ctgggtttcg
    20401 aggatccgca ccacgctgct gtagtgtggg agcatcattt ccaggagctt ctacatctga
    20461 ctgttccttt tcccggtatt gaggaaacac tggaagtgtt gcatgataat ggcattgtga
    20521 tgggggtggt cacttcgcgt aaccgtgcag aattcgaatc ggacccgtac atagcccgct
    20581 ggaaacattt cttcaccgtt acggtttgtg cagaagattc cctcgctcac aagccggcgc
    20641 ccgatcccat gctgaccttc atttcccgta cgggggccga accggcaggg gtgctattcg
    20701 taggcgacac gttttatgac caacagtgcg cacacgcagc cggcacttct tttgcactgg
    20761 ctacctgggg gagtcgcaac gagaaagata ttcctgccga acattacctg aatcacccta
    20821 cagatttgat aatataaccg gcatgttaaa acgctatatc tggcttatcc tgttcatacc
    20881 gctgcaggtg acagcacagg acattgatca tcctgcctca tcgctggcca ggatgcagga
    20941 ggctttccgg gtagaagcct caaagccgga tta
//
LOCUS       DAOSOF010000013        20915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2541_length_20915_cov_5.592330, whole genome shotgun sequence.
ACCESSION   DAOSOF010000013 DAOSOF010000000
VERSION     DAOSOF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_20915_cov_5.592330"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLM68_01510"
     CDS             complement(<1..398)
                     /locus_tag="PLM68_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54081.1"
                     /translation="MQQNKGKRTGIWLTVGLHLLLSVVLLLNGLHYVPPLRNNLMEIE
                     IELEEELPPLPLTIIAEQGTAPRSPEPVPDKAPELVQQAIVPEEIQGKQRTEESTPEE
                     TGDVEVHDPEPPVINERALYRSRDADTTEG"
     gene            complement(420..833)
                     /locus_tag="PLM68_01515"
     CDS             complement(420..833)
                     /locus_tag="PLM68_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT54082.1"
                     /translation="MAIKRNTKVEPSFTMASMTDIVFLMLLFFLVTSTLVNPNALKLL
                     LPKSSNQISDKAQVSVSIKHYTLAGTFSYHINGNQQAVPFNDIEPLLQQALQSSDDPT
                     VSLYVDKSVPMEQVVQVMNIAKRNQYKIILATSPE"
     gene            complement(840..1508)
                     /locus_tag="PLM68_01520"
     CDS             complement(840..1508)
                     /locus_tag="PLM68_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT54083.1"
                     /translation="MLHIVLQTEAIAEASSSISLLSMATKGGWLMLVLLALSIVAVYI
                     FGERLVAMHKASKVDENFMNNIKEFIYEGKIQSAIHLCESQNTVSSRLIAKGLRNIGR
                     PMADIKSVLENVGSIEVARLEKGMPVLASVAGGAPMVGFLGTVIGMIKAFYNMSMAGS
                     NIEISLLSEGIYIAMVTTVGGLLVGIPAYFAYNYLASRIGSIINTMEKNTLDFLELLQ
                     EPLK"
     gene            complement(1510..2289)
                     /gene="truA"
                     /locus_tag="PLM68_01525"
     CDS             complement(1510..2289)
                     /gene="truA"
                     /locus_tag="PLM68_01525"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT54084.1"
                     /translation="MRFFVHLSFNGTHYHGWQIQKNARSVQAFLEDALGRVLRETVAV
                     TGCGRTDTGVHALDFWAHFNYNGKPFGCEALTYKLNAILPNDIHIFGIFHVPENLHAR
                     YHAVSRTYRYYIRRNKEPFLEGMSSAPGTYAATPPDLDLMNQGAALFLGTHDFTSFSK
                     LHTQTVSNTCTVTEARWFTQEQEFGMAPLWVFQVTANRFLRNMVRCMVGTLLEVEKGK
                     KPVEWILQLLDGKNRSLAGFSVPPEGLFLWKVHYDKLIIVN"
     gene            2353..3345
                     /locus_tag="PLM68_01530"
     CDS             2353..3345
                     /locus_tag="PLM68_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT54085.1"
                     /translation="METVNIKELNERIQRESAFVDIMTLEMSKVIVGQKQLVENLLIG
                     LLANGHILLEGVPGLAKTLAIRTLASVVDAKFSRIQFTPDLLPADLIGTLIYSQRTEE
                     FQVKKGPLFANFILADEINRSPAKVQSALLEAMQERQITIGEHTFPLPEPFLVLATQN
                     PIEQEGTYPLPEAQVDRFMLKVIVDYPKKDEEKLIIRMNNSGEFPTAATVLKPADIRK
                     AREVVREVYMDEKIEKYIVDLVCATRRPEEYKLPELKSLIAYGSSPRASISLAIASKA
                     YAFIRRRGYVIPEDVRAVAYEVLRHRIGLTYEAEAENIVSENIITSIINAVEVP"
     gene            3355..4236
                     /locus_tag="PLM68_01535"
     CDS             3355..4236
                     /locus_tag="PLM68_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT54086.1"
                     /translation="MSENLLKNVRKIEIKSRGLSHQIFAGQYHSAFKGRGIAFSEVRE
                     YQYGDDIRDMDWNVTARMNAPYIKVFEEERELTVVLVIDVSGSRWFGTTKKLKKELLA
                     EVAAILSFSASINNDKVGALFFSDKVEKFIPPKKGRSHLLRIIREMLEFEPESRQTNI
                     SEALRFLTNAIKKRCTAFILSDMMDIDNAEQPRFEEALKIAVNKHDLAAIQVYDPREK
                     ELPNVGLVRMMDAETYREQWVDTSGKRLREDYAMWYRNSQERIKTLLNKYHVDHVKIA
                     TDQDYVEPLIRLFKQRA"
     gene            4236..5246
                     /locus_tag="PLM68_01540"
     CDS             4236..5246
                     /locus_tag="PLM68_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54087.1"
                     /translation="MMKRFLLQWFFPALLIMMPHISVARQDPATVQGKGDSVLGSVLE
                     KDSILIGDQVWWNMTLPPGIWTEGIIETPEFPSLPFEITRGIEALSGVRLDSVFRKKN
                     LESIRARILLTSFDSGSYQLPPVPVRLLRQDGTADTLWFKGPLLKVQTIPVDTTSFEP
                     YGLKPQMRYPITTGEILALAGIVALVTGLVFLALYIIRRRRKNLPLFGPVRPKDPPHI
                     AALKVLENMRRQEIWKKQKAKLYYTLLTDTIRVYLRDRWGMQAMELTSGEMIASLEQR
                     QPEDPLLSGKIIEVLREMLSMGDLAKFAKYTPSESENEESLRQAVRFVTETARYEEEK
                     EE"
     gene            5258..6247
                     /locus_tag="PLM68_01545"
     CDS             5258..6247
                     /locus_tag="PLM68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT54088.1"
                     /translation="MFFEYPRLLFLLLLLIPLAAWYVYRQMKGKQDPSLQVSSLQPFE
                     KEKVSYKRILYHVPFVLRMIALAALILAMSRPRSSSDYETTVTEGIDIMLAMDVSTSM
                     LARDFEPDRIAAAKDIAIQFIAERPADRIGITVFAGESYTQCPLTTDRITLINMMKEI
                     QTGLIEDGTAIGNGLATAVARLRDSEAISRVVILLTDGVNNRGEVTPLTAAEIARTYG
                     VRVYTIGVGAHGMAPYPVMTPYGVQIQQIPVEIDEELLRQIADITGGEYFRATDNTKL
                     MSIYSQINQMEKSRTLVDSFPVYRELFMIFALSALFALALEFLLRWIVLRRIP"
     gene            6254..7975
                     /locus_tag="PLM68_01550"
     CDS             6254..7975
                     /locus_tag="PLM68_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT54089.1"
                     /translation="MIQLAQVQYLWIIAVIPLLLVVYGLYRRYGKRMLRRFGDPELLK
                     MLMPSVSRRRGWIKISLFCAGLFFFALGLSRPRTGARVKEIKREGVEIIIALDVSNSM
                     LAQDYTPNRLERAKLAVSRLADNLGRDRIGLIVFAGDAFVQLPVTTDYVSAKLFLNTI
                     NTGSVPRQGTALGNAILTGIRSFSLDTPGSRALILITDGENHEDDAVAAAGKAANQGI
                     QVHCIGIGSEFGKPIPLADGGLLQDEEGNTVVTKLDEKTLMEVARAGGGIYVRADNSD
                     FGLQGIVDSIRQMDQQEFQAVVFEDFNEQYMYLFGIAFFFFFLETLIGERKGRRFKSL
                     DIFTKTAMVFVLFSALSITASAQADKKDVRAGNRSFRKGNLEKALLDYHRAVSRDSTS
                     LKARYNLANTMHLSGDDQQASSTMATVPDSLNIPEKKADAWHNLGNFQLSAKQYAESI
                     EAYKKSLRIRPGDMETKTNLAYAQKMLKDQQEKQDQQQQDQDQDKKDQDQNQDRQQQD
                     QNQDKQDQNRDQQQQDQNQVTPPKISPQAAQQMLEAVLQKERETQEKVNKEKARAAVP
                     VRSGKNW"
     gene            7987..9807
                     /locus_tag="PLM68_01555"
     CDS             7987..9807
                     /locus_tag="PLM68_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOT54090.1"
                     /translation="MKKTFLLILAVLSLVQATAQGQFTVEVPNVVASKERFRLVFTAT
                     DLAHESFSPPSISGFLVLAGPTTSTMNRMEYINGRRTQSRSVSYTYILEAGEPGKYTI
                     GEASILSDNVTYRTKPVTIEVIAGTAAPDSPAREENITTEANITDKDVFLRLSINKRR
                     VVKGEPLTATLKLYTRVNVAGAEDIRFPTFNGFWSQEVYAPQQIEWQRENVDGEIYQA
                     ATLRSYVLLPQQTGDIRIDPSEIVCVIQVRNTRRGQSLLDEFFDSYQTVRKRVIAPAL
                     TVRVNDLPAGAPASFTGAVGKFNLRAELGKDSVSAHDALSLFVTISGEGNINLLETPR
                     VQFPPDFEVYDAKVTDNSRSTGGTFSGSKTFEYPVIPRSHGDFTIDPVTFSYYDIEAG
                     VYRTISSRPLELKVSRGSQTPAQGMPYTPGTHRRAVESLGQDIRFIHTSVQPWRVKGR
                     YFTGSVYYFVLILSIFAMAGVAYVVLDKRRMHRNDIVKVRHSRANKVARNRLKTAGTL
                     LKQEQFEDYYEELQRALWGYIADKLALSQAECSRERVVDMLGSRNVDKSLVNDYTGLI
                     ESCEFARYAPDPGQLEKERIFDMAVVAISKMEQALKQIVV"
     gene            9804..10568
                     /locus_tag="PLM68_01560"
     CDS             9804..10568
                     /locus_tag="PLM68_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54091.1"
                     /translation="MKRFLLLFVLCFTASLTAGAKEVTDSLWNEANTHYSNAQYQEAL
                     DKYLSIEESGKESPALYYNIGNTYFQMRYIAYAILYYEKALRLEPNNPDIIYNLEVAR
                     EQCIDRIEPVPVFFLADWIRKSHQLLSSDIWTAISIILLVSALVFFMIFLFARGVRFR
                     KASFILSVVILLFTLLTVALAWQSRKEATREDLAIVLSAVSSVRSSPGTQGKDLLVIH
                     EGTRLRVLEKMGDWARIELEDGRQGWVALNEIVFVY"
     gene            10590..11237
                     /locus_tag="PLM68_01565"
     CDS             10590..11237
                     /locus_tag="PLM68_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOT54092.1"
                     /translation="MDYNTQREKLILPEYGRYIYQMVQDAKKIPDREARTRQVKAIVN
                     AMSLLNPQLRDMNDYQHKLWDHVYIMADYELDVDAPFPPPAREDFVTKPHAIDAERDP
                     VKVMHYGRNIQNMVMAIASKEPGEERDTMTLALAAYMRKQYLIWNKDTVSDQTIFNDI
                     RSLSNGTLTVPEGAKLSEIQGEFRQPNVLQGARTQQRQGQWHKNKKKKRSTSNGQ"
     gene            11227..13470
                     /locus_tag="PLM68_01570"
     CDS             11227..13470
                     /locus_tag="PLM68_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT54093.1"
                     /translation="MASDKEKKNRIRDNIRIISGWLFLALGAFLLVAFVSYLFNWTVD
                     QSLLSGDSLFMPEDSAANSGGFIGNRWADLFIGKLFGLSAFILPFFCLAVSVFLLKIR
                     SVRIIPLFFATAFLCIILSIALGYIFSFTDLRYVFGAGVGGTYGYRVNEWLMGMLGTV
                     GAGVVIFFVLTAIPLPLVYRWIRKRKERRLEAALAEPAGTEPEHGIAPESIVEPALKK
                     APIEIPPDPALHRPVFEVEKTEEISTSGKMVFYDPKQDLSHYKKPTLDLLEDYKNRIT
                     EVPQEELERNNQKIVECLRTFDIQIEKIVATPGPTVTLYKIVLSPGIRISQFTRLEDD
                     IMLSLAARGMRVIAPIPGTNMVGIEVANDKPSLVPMKSVLDSTKFKESKDDLPIVLGK
                     SISNEIVVLDLARLPHLLVAGATGQGKSVALNAILTSLLYKMHPAYLKLVLVDPKRVE
                     LNLYSKLENHFLARLPDADSSIIIDTKKVVYTLNSLCIEMDNRLELYNHAGVRNIKEY
                     NTKFQERILNPLKGHRFMPFIVLIIDEFADLIMTAGRDVEGPLTRLAQMGRATGIHLV
                     IATQRPTTNIITGTIKANFPARMAFRVASKIDSRTIIDVSEASQLVGRGDMLISAGTD
                     LQRVQCAFIDTPEVERMVDFIGNQPGYSGPFELPEYSETERQDAIYPSSGQRDELFEE
                     AARLLVQNGEGSTSLLQRRFHLGYNRAGRIMDQLEQAGIVGPPRGSNPRELLVSSLDM
                     LEDILSK"
     gene            13473..14060
                     /locus_tag="PLM68_01575"
     CDS             13473..14060
                     /locus_tag="PLM68_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT54094.1"
                     /translation="MQAFISGILMLVTALIPGAQTTESLLNSFSQQLAKAPAMEIEFL
                     WNETVSGTMVLQGRMFTLHMDEFHIYCNGEKKWYYNQGIDQWEEMPHDSNSPDILENP
                     SAFFSRLNEDYYHPDTPVKKTSMAGDPVWELTLVPNSIESPFTYVILGITVEGLKPLY
                     IRYALTDGTAYPVKINSFKTVSARPESFFLPGFSR"
     gene            complement(14031..14951)
                     /locus_tag="PLM68_01580"
     CDS             complement(14031..14951)
                     /locus_tag="PLM68_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT54095.1"
                     /translation="MKILTPEQYQKKGMRLQKMEKMASRAMYHAMIKRNKALRKEVFM
                     ETRHGHTRTVWYGFENPSPGPLLIDLHGGGFILGSPEMDERMNIEWSRLLGCRIISID
                     YPKAPGHPFPAAVDQVYAILEYLWEHAKVYGIDKARIAIGGHSAGANLTAVTCMKAKK
                     EGKIQPVCQFMNCPPLDMATSAIEKPLPKGCIPPKFAYMFDSSYLAGNDGHDPNISPV
                     YAQDEDLKGLPPALIIVAGRDSLHDEGVRYYNRLTENGVDAQLLDYPFGRHCFMLKKS
                     TLTTDAFEKVTRFLQQHLLGVSAGESGQKE"
     gene            complement(14955..16262)
                     /locus_tag="PLM68_01585"
     CDS             complement(14955..16262)
                     /locus_tag="PLM68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOT54096.1"
                     /translation="MTEFRHMMEASLMPGDVDPLLSCLDSQPTVSIRQHALKAAGELF
                     PGTDTVPWCPLGRYLDERPVFTLDPRFHAGAYYVQEASGMSLWQLGPYLDNITSPRVL
                     DACAAPGGKSTLLLDLMHGEGLLVSNEVIRTRVPILAENLAKWGYANVVVTQNDPADF
                     GKLPEYFDVVVADVPCSGEGLFRKDPDTRKTWSPGLVALCVARQRRILSDLWSSLRRG
                     GLFLYSSCTLNRMEDEEQIDWIRDHLGARLLLGPQKYLPHRVRGEGFFMALLEKNGPR
                     EAGVKKILPAPDGKKGWTPYRVPERAPLHLEGSYSWSMKGQMLKAFPRESHREMEALG
                     SMLHVFHSGIAIATKKGDDWVPWADLALATDLKKDAYPVVVIDREQALAFLSKNPLVL
                     PQGTEKGYLLLIYNGLPLGFVKNLGKRSNNLHPVSRRIHLSIQ"
     gene            complement(16285..16692)
                     /locus_tag="PLM68_01590"
     CDS             complement(16285..16692)
                     /locus_tag="PLM68_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOT54097.1"
                     /translation="MGNTHIEGNPCLVNKPASVVYHLFSDFSRFSERLPEDKKDKVII
                     TKDTILAEAQGMQIGVKVTKREEPSLIVMEQHGNVPFAFNLNLHIRPREEGCELQLFL
                     DAELNMMLKMMLGGKIKDFVNQFTTQLAEGLNK"
     gene            complement(16695..17327)
                     /gene="pyrE"
                     /locus_tag="PLM68_01595"
     CDS             complement(16695..17327)
                     /gene="pyrE"
                     /locus_tag="PLM68_01595"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT54098.1"
                     /translation="MESVEKKVAQILLEIEAVQLNIERPFEWASGWLSPIYCDNRKVL
                     SVPTVRNFICDALCRSIEQNYPTADVIAGVATGAIAMGALIADRLGKPFIYVRPRRKD
                     HGTGALIEGTLTPGSNVLVVEDLISTGHSALSAADCLRMACANVLGVVATFSYNFDVA
                     RRAFEKSNCELHTLTNYNTLIQEAAERNFVSPEQLETLKIWRLTPNTWGR"
     gene            17351..18025
                     /locus_tag="PLM68_01600"
     CDS             17351..18025
                     /locus_tag="PLM68_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOT54099.1"
                     /translation="MYSIFFNNRRLQLCRPDNPVCLLPSVTVESFENHSSWEELIVSF
                     QCNTSLRHVVVPVQNPHDSFMQICKNFTIMPAAGGLVTNKQDDLLMIYRYGKWDLPKG
                     KQEQGEKLSDTALREVAEETGTIDLKLLGDQLTTTLHCYHYKGNNVLKMTAWFPMTAN
                     SGTTLLPQAEEGIEKAEWVSRKDLAERLDMSYLSIKILVEKQFLNDCKNVGEACKIED
                     ILDLCP"
     gene            complement(17960..18259)
                     /locus_tag="PLM68_01605"
     CDS             complement(17960..18259)
                     /locus_tag="PLM68_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT54100.1"
                     /translation="MEVIISNDPVPVISINGRMDTGNTAIFEEAIVPILKSNTRELII
                     DGTGLEYISSSGLRQFLVLQKNIKEKGGTLTVKNLRAEIKDIFDLTGFTNIFTIV"
     gene            complement(18317..19564)
                     /gene="nqrF"
                     /locus_tag="PLM68_01610"
     CDS             complement(18317..19564)
                     /gene="nqrF"
                     /locus_tag="PLM68_01610"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOT54101.1"
                     /translation="MTIFQFLFLTFAVILTVTLVLVVVLIYAKARLTPGGKVKIKVND
                     EKMLEVEPGNNLLTTLAQNKIYLPSACGGSGTCGLCKCRVLSGGGSILSTETGFFTRR
                     EQQSQWRLGCQVRVRENMEIHIPAQVLGVKKWECEVISNRNVASFIKEFVIQLPPGEK
                     LDFRSGGYVQIDVPPVTVDFSKDIAIDELFREDWSSLGIWGLKMVNKEYSFRAYSMAN
                     HPAEGNRIMLNVRIATPPWDRSRGTFMKVNPGICSSYIFSRKPGDKVMISGPFGEFFL
                     KETQAEKIFIGGGAGMAPMRSHIFHLFKTLRATDKVSFWYGARSRREIFYEDEFLRIA
                     KEFPNFSFHIALSEPRPQDHWTGHTGFIHKVLYDQYLKDHPEPEEAEFYICGPPLMTS
                     AVTEMLDNLGVPADHILFDDFGG"
     gene            complement(19574..20191)
                     /gene="nqrE"
                     /locus_tag="PLM68_01615"
     CDS             complement(19574..20191)
                     /gene="nqrE"
                     /locus_tag="PLM68_01615"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOT54102.1"
                     /translation="MEQIANIFVKSIFTENMIFAYFLGMCSYLAVSKTVKTASGLGLA
                     VIFVLLITLPVNYLLDRYVLSPGALAWINPQWAEVDLGFLSFILFIAVIAAMVQLVEM
                     VVEKFAPALYNNLGIFLPLIAVNCAILGASLFMQEREYGNILEVFSYALGSGIGWYLA
                     IVSIAAIREKLSYSNIPAPMRGLGIAFIITGLMGIAFMSFMGLQT"
     gene            complement(20195..20809)
                     /locus_tag="PLM68_01620"
     CDS             complement(20195..20809)
                     /locus_tag="PLM68_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOT54103.1"
                     /translation="MNKRIFLDPLFKNNPIPVLALGICSALAVTVKLEPAMVMALSVT
                     LVTGFSSMILSLLRNTIPNRIRIIVQLVVVAALVILVDQILKAFAWEISKQLSVFVGL
                     IITNCIIMGRIEAFALYNKPFPSLVDGLANGLGYGIILIAVSIVRELLGSGTLIGYQV
                     IPQQWYVANGGFYENNGLFILPPMALIILGLIVWVFNAKNAKRV"
     gene            complement(20802..>20915)
                     /locus_tag="PLM68_01625"
     CDS             complement(20802..>20915)
                     /locus_tag="PLM68_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT54104.1"
                     /translation="NPNAVDGISGGTLTSRGVENMIRESLKPYLPFLKHYE"
BASE COUNT         5540 a         4992 c         5248 g         5135 t
ORIGIN      
        1 tcaccttcgg ttgtgtctgc atcccggctt ctgtacaagg ccctttcatt gataaccggg
       61 ggttcaggat cgtgtacttc aacgtcaccg gtttcttccg gcgtgctttc ctctgtgcgt
      121 tgcttgccct gtatctcttc gggaacaatt gcctgttgaa ccaattcagg tgccttgtcc
      181 ggcacaggtt ccggggagcg gggcgcagta ccttgttcgg caataattgt gagaggtagc
      241 gggggtaact cttcctccag ttcaatctct atctccataa gattattgcg caggggaggg
      301 acgtaatgca atccgttgag cagcaaaaca accgacagca gcaggtgaag tcccactgtc
      361 agccagatac cggtcctttt tcccttgttt tgttgcatag atgcatgttt tgttgctcct
      421 tactcaggag atgtggccag aatgatcttg tactgattac gtttggcaat gttcatcacc
      481 tggaccacct gttccatggg aacagattta tccacgtata aagataccgt gggatcatca
      541 ctgctctgaa gggcttgttg cagcagtggt tcaatgtcat tgaaagggac agcctgctgg
      601 ttaccgttga tgtggtatga gaaagtacct gccagggtat aatgcttgat ggatacacta
      661 acctgtgcct tgtccgagat ctggttggag ctttttggca gcaatagctt cagggcgttg
      721 ggattgacca gggttgaggt aaccaggaag aacaacagca tcaaaaacac aatgtctgtc
      781 atggaagcca ttgtgaaaga cggctcgacc ttggtgtttc tttttatagc catgtcattt
      841 tattttagcg gttcctgtaa aagttccaga aaatccagcg tattcttttc catggtgtta
      901 atgatggacc cgatacgtga agcaaggtaa ttgtatgcaa agtaagccgg tatgcccacc
      961 agcaatccgc ccaccgtggt aaccatggct atatagattc cctctgataa cagcgatatc
     1021 tcaatgttac ttccggccat ggacatattg tagaaagcct tgatcatacc gatgaccgtt
     1081 ccaagaaacc cgaccatggg cgcgccaccg gcaacggaag ccagtacagg cattcctttt
     1141 tccagccggg caacctcaat gcttcccaca ttttccagga cagatttgat gtcggccatt
     1201 ggccgtccaa tattacgcaa tcccttggct ataagccttg aggatacagt attctggctt
     1261 tcgcacagat gaatggccga ctgtatcttt ccttcatata tgaattcctt gatattgttc
     1321 atgaaattct cgtccacttt ggatgctttg tgcatggcaa caagtcgttc tccgaaaatg
     1381 taaacggcta caatggataa tgcaagcagg accagcatca gccaaccgcc tttggtagcc
     1441 atggacaata atgaaattga agaagaggct tctgcaatgg cctcggtttg aagtacgatg
     1501 tgcaacatat cagtttacaa ttataagttt atcataatga accttccaca ggaacaagcc
     1561 ttcgggtgga acggaaaaac cagccaggga acggtttttt ccatccagta actgcaaaat
     1621 ccattccacg ggttttttac ctttttccac ctccagcaat gtacccacca tgcaacggac
     1681 catgttccgg aggaaacggt tggcagtgac ctggaagacc cacaacgggg ccattccgaa
     1741 ttcctgctcc tgggtgaacc agcgggcttc ggtaacagtg caggtgttgc tcacggtttg
     1801 tgtatggagt ttggaaaagc ttgtaaaatc atgagttccc agaaacaatg cagctccctg
     1861 gttcatcagg tccaggtcgg gaggcgtagc ggcgtatgtt cccggagcgg aagacatacc
     1921 ttccaggaat ggttctttgt tcctgcggat ataataacgg taagtcctgc tcacggcatg
     1981 gtaccgggca tggaggtttt caggtacgtg aaaaatgccg aatatatgaa tatcattagg
     2041 caatatggca ttcaatttgt aagttaaggc ttcacacccg aaaggtttac cgttgtaatt
     2101 gaaatgagcc cagaaatcaa gcgcgtggac acccgtgtcg gtgcggccgc atcctgtgac
     2161 tgcgactgtc tctctcaaaa cacgtcctag cgcatcttcc agaaaagcct gaacgctacg
     2221 tgcgtttttt tgaatttgcc agccatggta atgcgtaccg ttaaaggaaa gatgaacaaa
     2281 aaaccgcata aaggatgtat ctttgacaaa tatatatgaa aaaaacataa tcgtatagaa
     2341 aaataacatt gtatggaaac cgtaaacatt aaagaattga acgaacggat tcagcgggaa
     2401 agtgcttttg tggatatcat gacgcttgaa atgagcaagg tgatagtagg gcagaaacaa
     2461 ttggttgaaa atctgcttat tggacttctg gccaatggac acatcctgct ggaaggcgtt
     2521 ccgggacttg ctaaaacgtt ggcaataaga acattggcaa gtgttgtgga cgcaaagttc
     2581 agccgcattc aattcactcc cgacttgttg cctgccgacc tgataggtac cctgatttac
     2641 agccagcgaa cagaggaatt tcaggtaaag aaaggccctc ttttcgctaa tttcattttg
     2701 gctgatgaaa tcaaccgctc tccggcaaaa gtgcaatcgg cccttctgga ggccatgcag
     2761 gaaagacaga tcaccattgg agaacataca tttcccctgc cggaaccttt cctggtgctg
     2821 gcaacccaga atcccattga acaggaaggg acgtatcctt tgcccgaggc acaggtggac
     2881 cgcttcatgc tcaaggtcat tgtggattac cccaaaaaag acgaagaaaa actcatcatc
     2941 cgcatgaaca attccggtga atttccgaca gcggcaacgg ttctcaaacc tgccgacatc
     3001 aggaaggccc gggaggttgt ccgggaagtt tacatggatg agaaaattga aaagtatata
     3061 gtagacctgg tttgtgccac ccggagaccg gaggagtaca aactgcccga gctcaaatcc
     3121 ctgatagctt acggttcttc cccgcgtgct agtatttccc ttgccatcgc ttccaaggcc
     3181 tatgccttta tccgccgccg tggctacgtc attccggaag atgtccgtgc agtggcctac
     3241 gaagtgttac gccacaggat aggactgact tacgaggcag aagccgaaaa cattgtctct
     3301 gaaaacatca tcacttcaat tatcaatgcg gttgaagttc cttaatagcc ggctatgtct
     3361 gaaaatttgc tcaaaaatgt ccggaagata gagatcaaat ccaggggtct ttcgcaccag
     3421 atctttgcag gccagtacca ctcagctttc aagggccggg gcattgcctt cagcgaggtg
     3481 cgtgaatacc agtacggaga cgacatcagg gacatggact ggaacgtcac ggcaagaatg
     3541 aacgccccct atatcaaggt ttttgaggaa gaacgtgaac tgaccgtagt gctcgtcatc
     3601 gatgtgagcg gctcgcggtg gtttggcaca acaaagaaac tgaagaagga attgctggcc
     3661 gaggtggctg ccattctcag tttctctgcg tccatcaaca acgacaaggt gggagcgttg
     3721 tttttcagcg ataaggtgga aaaattcatt cccccgaaaa aaggacgttc acacctgttg
     3781 cgtatcattc gcgaaatgct ggaattcgag cccgaaagcc ggcagacaaa catatctgaa
     3841 gccttgcgct ttctgaccaa tgccataaaa aaacgttgca ctgcctttat cttgtcagac
     3901 atgatggata tagacaatgc agaacaaccc cgttttgaag aagcactgaa gatagccgtg
     3961 aacaagcacg atctggcggc catacaggtt tatgatccaa gggaaaaaga attacccaat
     4021 gtaggcctgg tccggatgat ggatgccgaa acataccggg aacaatgggt agacacctcg
     4081 ggaaaaaggt tgagggagga ttatgccatg tggtacagaa acagtcagga gagaattaaa
     4141 accctcctga ataaatacca tgttgatcat gttaaaattg ctactgacca ggattatgtg
     4201 gaacccctga tccgcttgtt taaacaaagg gcgtgatgat gaaacgtttt ctgttacaat
     4261 ggtttttccc ggccttgctc attatgatgc cacacatatc agtggcccgg caggaccctg
     4321 ctacagtaca gggaaaggga gattctgtcc tgggatcggt tctggaaaag gatagcattc
     4381 tgatagggga ccaggtctgg tggaacatga cattaccacc cggaatatgg accgaaggaa
     4441 taatcgagac acctgaattc ccatcgcttc ctttcgagat caccaggggg atcgaggcct
     4501 tgtccggggt gcggctggat tccgtattca ggaaaaaaaa cctggaaagc atccgggcgc
     4561 gtattctgct gacttcgttc gacagcgggt cctatcagct gccgcccgtt ccggttcgtc
     4621 tgctacggca ggatggtaca gctgatacct tgtggttcaa aggacccctg ttaaaggttc
     4681 agaccattcc ggtagacacc acgtcttttg aaccttacgg gctgaagccc cagatgcgtt
     4741 atccgataac aaccggagaa atactggccc ttgccggtat tgtcgcactg gtgacaggcc
     4801 tggttttttt ggcgctttat attataagac gccgcaggaa gaatctgcct cttttcggac
     4861 ctgtcaggcc taaggacccg cctcacattg cagccctcaa agtgctggaa aatatgagga
     4921 ggcaggaaat ctggaaaaag cagaaagcaa aactatacta taccctgcta acagacacca
     4981 tccgggttta cctcagggac cggtggggga tgcaggccat ggaactgacc tcgggcgaaa
     5041 tgatcgcatc gctggaacag aggcaacctg aagatccgct gctctccggg aagatcatag
     5101 aagtgctccg ggagatgtta agtatgggtg acctggcaaa atttgccaaa tacacaccgt
     5161 cagaaagtga aaacgaagaa tccctcaggc aggccgtccg gtttgtaaca gaaacggcca
     5221 ggtatgaaga agaaaaagaa gaataaggag aaccattatg ttttttgaat atcccagact
     5281 cctgttttta ttgctcttgc tgatcccttt ggcagcctgg tacgtatacc ggcaaatgaa
     5341 agggaagcag gatccttcgc ttcaggtatc ttcgctgcag ccttttgaaa aagagaaggt
     5401 ttcctataag agaatccttt atcacgtgcc ctttgtactg aggatgattg ccctggccgc
     5461 cctgatactg gccatgtcca ggccgcgctc ttcctcggat tatgaaacaa cggtcaccga
     5521 agggatagac atcatgcttg ccatggacgt gtccacttcc atgctggcac gtgattttga
     5581 gccggacagg attgcagctg cgaaggacat tgccattcag tttattgcgg aacgtcccgc
     5641 agaccgcata ggcataaccg tatttgcggg tgaatcgtat acgcaatgtc ccctgacaac
     5701 agaccgcatc acgctcataa acatgatgaa ggaaatccag acaggtctga ttgaagacgg
     5761 aacggccatc gggaacggac tggcaacagc cgtggcccgt ctaagggatt cggaagccat
     5821 tagccgggtg gtcatcctgc ttacagacgg ggtgaacaac agaggcgagg tgacgccact
     5881 cacagcagcc gaaattgcac gcacttacgg ggtgcgggtg tatacaatag gtgtgggagc
     5941 gcatgggatg gctccctacc cggtcatgac gccttatggc gtgcagatac aacagatacc
     6001 ggtggaaatt gatgaggaac tgcttcggca gattgcagac attaccggtg gtgagtattt
     6061 tcgcgccacg gacaatacaa agttgatgtc catttacagt cagatcaacc aaatggaaaa
     6121 aagcaggacc ctggtggatt catttcccgt atacagggaa ttgttcatga tttttgccct
     6181 gtcagccctt ttcgccttgg cactggaatt tttattgcgt tggatcgtat tgagaagaat
     6241 accctaagga gatatgatac aattggctca agttcaatat ttatggatca tagcagtcat
     6301 cccgctgctg ttggttgtat atggccttta ccgcaggtac ggaaagagga tgctaagacg
     6361 gttcggggat ccggaattac tcaaaatgct catgccatcg gtatcacgcc gcaggggctg
     6421 gatcaagata agcctctttt gtgccggctt gtttttcttt gccctgggtt tgtcgcgtcc
     6481 ccgtaccggg gcccgtgtca aggaaataaa acgtgaaggt gtggaaatca tcattgcgct
     6541 ggatgtttcc aattcaatgc tggcccagga ttataccccc aacaggctgg aacgggccaa
     6601 actggccgtt tcgcgcctgg cggacaacct gggcagggac aggataggcc tgattgtttt
     6661 tgccggagat gcattcgtgc aacttcccgt aacaacggat tacgtcagtg ccaaactttt
     6721 cctgaatacc ataaataccg gatcggtccc ccggcaggga acggcactgg gcaatgccat
     6781 cctgacaggt atcaggagtt tcagcctgga tacacccggt agcagggccc tgatcctgat
     6841 tacagacgga gaaaaccacg aggacgatgc tgtggcggca gccgggaaag ctgcaaacca
     6901 gggaatccag gtgcattgca taggaattgg ctccgaattc gggaaaccaa tacctttggc
     6961 cgacggagga ttgctccagg atgaggaagg aaatacagtg gtcacgaaac tggatgagaa
     7021 aacattgatg gaagtggccc gggcaggcgg aggaatctat gtcagggcag acaattcaga
     7081 tttcgggctt caggggatcg tggattcgat acgtcaaatg gaccagcagg aattccaggc
     7141 agtagtattt gaagatttca atgagcagta tatgtacctg tttggtatcg cattcttttt
     7201 ctttttcctg gaaacgctca tcggagaaag gaaagggcga cgtttcaaaa gtttggatat
     7261 ttttacgaaa acagcgatgg tctttgtgtt gttctctgct ctctccatca cagcctcggc
     7321 ccaggcagat aaaaaggatg tccgggcggg taacaggtct ttccgcaagg gaaacctgga
     7381 aaaggcgctg ctggattatc acagggctgt atcaagggat tccacatccc ttaaggcgag
     7441 atacaacctg gcaaacacca tgcacctgag cggagacgac cagcaagcct cttctaccat
     7501 ggccactgtc cccgattccc tgaacattcc ggagaaaaaa gccgatgcat ggcacaactt
     7561 gggaaatttc cagctgtctg ccaaacaata tgccgaaagt attgaagcct ataagaaatc
     7621 attgcggata cggcccgggg atatggaaac aaagaccaac ctggcatatg cccaaaaaat
     7681 gctgaaagac cagcaggaga agcaggacca gcaacagcag gaccaggatc aggacaagaa
     7741 ggaccaagac cagaaccagg accggcaaca acaggatcag aaccaggaca agcaggacca
     7801 gaaccgggac cagcaacagc aggatcagaa ccaggtgaca ccaccaaaaa taagccccca
     7861 ggctgcgcaa cagatgctgg aagccgtgct gcagaaagag cgtgaaacac aggaaaaggt
     7921 gaacaaagaa aaggcccggg cagcagtacc tgttcgttcc gggaaaaact ggtaaacaca
     7981 ggaaatatga aaaagacatt tttgttgatt cttgcagttt taagcctggt gcaggcaacg
     8041 gcacagggac agtttaccgt tgaagttccc aatgtggtag cctcgaagga gagattccgc
     8101 ctggtattta ccgcaacaga tctagcccac gaatctttca gtccaccgtc aatttccggt
     8161 tttttagtat tggcagggcc aaccacttcc accatgaaca ggatggaata tatcaacggg
     8221 cgcaggacac aaagccgttc agtcagttac acatatatcc tggaagcggg tgaaccggga
     8281 aaatatacca taggggaggc ctccatcctg tcggacaatg tgacatacag gactaaaccg
     8341 gtaaccatag aagtgattgc cggaacagcg gctcctgaca gtccggcacg cgaagaaaac
     8401 attacaacag aggcaaacat tacagataag gacgtcttcc tgcggctttc cataaacaag
     8461 cgccgggttg tgaagggaga accgctgaca gccacgctta agctttacac acgggtgaat
     8521 gtggccgggg cagaagatat tcgtttcccc acctttaacg gtttctggag ccaggaggtc
     8581 tatgcgcccc agcagattga atggcaacgc gagaatgtgg acggtgaaat ctaccaggca
     8641 gccaccttga gaagctatgt gttgctaccc cagcaaacag gggacatccg gattgatccg
     8701 tctgaaatag tatgtgtcat ccaggtaaga aatacgcggc ggggacaatc cctgctggat
     8761 gaattctttg attcctacca aaccgtacgc aaacgagtta tagcccctgc attgaccgta
     8821 cgggtaaatg atctgccggc gggagctcct gcctccttta ccggtgctgt gggaaaattc
     8881 aacctcaggg cagaacttgg aaaagattct gtaagtgccc acgatgcttt atccttattt
     8941 gtgaccattt caggagaggg gaacatcaac ctgctggaaa cgccccgggt tcaatttcct
     9001 ccggattttg aagtatacga cgccaaagtg acggataatt cacgcagtac aggaggaact
     9061 ttctcgggat caaaaacatt tgaatatcct gtaattccaa ggagtcacgg agatttcacc
     9121 attgacccgg tgaccttttc ctattacgac atagaggcgg gagtatacag gaccataagt
     9181 tcccgcccgc tagagctgaa agtttcaaga ggttcccaga caccagccca gggaatgcct
     9241 tatacaccgg gcacgcaccg ccgtgctgtg gaaagcctgg gacaggacat acggtttatt
     9301 catacttctg tgcagccctg gcgggtgaaa ggcaggtatt ttacagggag tgtttattat
     9361 tttgttctga tcctctccat atttgcaatg gctggagttg cttatgttgt tctggataaa
     9421 aggcgtatgc accgcaatga catagtgaag gtacggcaca gcagggcaaa caaggtggcc
     9481 cgtaaccgcc tgaaaacagc cggcacattg ttgaagcaag agcaatttga ggattattac
     9541 gaagaattgc aacgtgcact gtggggatat attgcagata aactggcttt atcgcaggca
     9601 gaatgttccc gggaaagagt ggtggacatg ctgggcagca gaaatgtgga taaaagtctg
     9661 gttaacgatt ataccggact tatagaatct tgtgaatttg cccgttatgc tccggatccc
     9721 ggacagctgg aaaaagaaag gatctttgat atggccgttg tagctatcag caagatggaa
     9781 caggccctta aacagattgt ggtatgaaaa ggtttttgct gttgtttgtt ttatgtttta
     9841 ccgcttccct gaccgcggga gcaaaggagg tgacggatag cctgtggaat gaagccaata
     9901 cccattattc caatgctcaa taccaggaag cgctggataa atacctcagt atagaggaat
     9961 cgggcaagga atcccctgcc ctttattaca atatcggcaa tacatacttt caaatgcgtt
    10021 atattgccta tgccattctt tattacgaaa aagccctgcg cctggaaccg aacaatcctg
    10081 atatcatata caacctggaa gtggccaggg aacagtgcat agacaggata gagcctgtcc
    10141 cggtattctt tctggccgac tggatacgca aaagccacca gcttttatct tctgacatct
    10201 ggactgcaat ttccatcata ttgctggtgt ctgctctggt cttcttcatg attttccttt
    10261 tcgccagggg agtgaggttc cgtaaagctt cctttatcct gtccgttgtg atcttgctat
    10321 ttacactact taccgttgct ctggcctggc aatccagaaa agaggcaacc agggaggacc
    10381 tggccattgt tctctctgct gtgagttctg ttaggtcttc acccgggaca cagggcaagg
    10441 atcttctggt cattcatgaa gggacacggc tgcgtgtgct cgaaaaaatg ggggactggg
    10501 cccggataga gctggaagac ggccgccagg gatgggtagc attgaatgag attgtttttg
    10561 tatattagta gcttgaaaat taattaaaaa tggattataa cacacagaga gagaaactta
    10621 tactgcctga atacggccgt tacatatacc agatggttca ggatgcgaaa aaaatccctg
    10681 acagggaagc acgtacccgg caggtcaaag ctattgtaaa cgccatgagc ctgctgaatc
    10741 cgcaattacg tgacatgaac gattaccagc ataaattatg ggatcatgta tacataatgg
    10801 cagattatga gctggatgtg gatgccccgt ttccccctcc cgcccgggaa gattttgtaa
    10861 caaaaccgca cgccatagat gctgaaaggg acccggtcaa ggtcatgcat tacgggagga
    10921 atatccagaa catggttatg gcaatcgcat ccaaggaacc gggagaggaa cgtgacacca
    10981 tgaccctggc ccttgctgcc tacatgcgta aacaatacct tatatggaac aaggatactg
    11041 tatccgatca aaccatattc aatgatatac gcagcttgtc caatggaacc ctgacggtac
    11101 ccgaaggggc taagttgtct gaaatccagg gcgagttcag gcagcccaac gtactacagg
    11161 gtgcacgtac ccagcagcgt cagggacaat ggcataagaa taaaaagaag aaaaggtcga
    11221 catcaaatgg ccagtgataa agagaaaaaa aaccgtatcc gggataatat aagaattatc
    11281 tccggatggt tgtttctggc cctgggagca ttcctcctgg ttgctttcgt ttcctactta
    11341 ttcaactgga ccgtggatca aagtctcctt tcgggagaca gcctgtttat gcctgaagac
    11401 agtgctgcca acagcggagg tttcataggg aatcgctggg ccgacctgtt cattggtaag
    11461 ctgttcggat tgagtgcctt catacttccc tttttttgcc tggctgtttc tgtatttctg
    11521 ctcaaaattc gcagtgtccg cattattcca ttattctttg caacagcttt cctgtgcata
    11581 atcctgtcaa ttgcattggg atacatcttt agttttacgg atctcaggta tgtgtttggg
    11641 gcaggggtag ggggaactta cggataccgt gtgaacgaat ggcttatggg catgctggga
    11701 acagttggtg ccggtgtggt tattttcttt gttctgaccg ccattcccct gccattagtg
    11761 taccggtgga tccgcaaaag aaaagagaga cgtctggaag ctgcccttgc agaacctgcc
    11821 gggacggaac ctgaacacgg gatagcaccc gaatccattg tggaaccagc tttgaaaaaa
    11881 gcacctatag agattccccc cgatcccgct ttgcatcgcc ctgtctttga ggtggaaaag
    11941 acagaggaaa tttccaccag cggcaagatg gtcttttatg atcccaaaca ggacctgtcc
    12001 cattataaga agcctacgct ggacttgctg gaggactata aaaacagaat aacggaagtg
    12061 ccgcaggaag aattggagcg gaacaaccag aaaatcgtgg aatgcctccg tacattcgat
    12121 attcagatag agaagattgt tgccacaccc ggtcccacgg ttaccttgta caagatcgta
    12181 ctctcgcccg ggatcaggat ctcacagttc acgcgcctgg aagatgacat aatgctttcc
    12241 ctggcagcac ggggtatgcg agttattgcg cccatccccg gaaccaatat ggtgggtatt
    12301 gaagtggcta acgacaaacc atccctggtg cccatgaaaa gcgtgctgga ttccacgaag
    12361 ttcaaggaat caaaagacga tttgcccatc gtcctgggaa aaagcatctc taatgaaatc
    12421 gtagtgctgg atcttgcccg cctgcctcac ctgctggtgg caggagccac aggacaaggt
    12481 aaatccgttg cgctgaacgc gatcctcacc tcgttgctct acaaaatgca tccggcctat
    12541 ctcaagcttg tattggtgga tcccaagcgg gtagagctga acctttattc caaactggaa
    12601 aaccacttcc tggccaggct tcccgatgca gatagttcca tcatcattga caccaagaaa
    12661 gtcgtatata ccctgaattc cctatgcata gaaatggaca accgcctgga attgtacaat
    12721 catgcaggcg tgaggaatat caaggaatac aatacaaaat tccaggaaag gatactcaac
    12781 ccgctgaaag ggcaccggtt catgcccttt attgtgctta tcatagatga gtttgccgat
    12841 cttatcatga cggccgggcg cgatgtggaa ggccccctaa cacggctggc ccagatgggg
    12901 cgagctacag gcatacacct ggtcatagca acgcaacgac ctaccaccaa catcattacc
    12961 gggaccatta aagccaactt cccggccaga atggcattcc gtgtggcttc aaaaatcgac
    13021 tcccgcacga tcattgatgt atctgaagcc agccagctgg tagggagggg tgacatgctc
    13081 atctcggccg gaacagacct gcagcgtgta cagtgtgcat tcatagacac accggaagta
    13141 gaacggatgg tagattttat cgggaatcag cccggatatt caggaccgtt cgagcttccc
    13201 gaatattcgg aaaccgaaag gcaggatgcg atttatccgt cttcgggaca gcgtgatgaa
    13261 ttgtttgaag aagcggcaag gttattggta caaaacggtg aaggttccac ttcgctgctg
    13321 cagcgcaggt ttcatcttgg ctacaacagg gcgggcagga tcatggatca gctggaacaa
    13381 gcagggatcg tgggtccccc aaggggaagc aatcccaggg aattgttggt ttccagcctg
    13441 gatatgctgg aggatatttt aagcaaatga caatgcaggc attcatttca ggcatattga
    13501 tgctggtaac ggcccttatt cccggagcac agactacgga aagcctgctc aacagttttt
    13561 cacaacagct ggcaaaagct ccggccatgg aaatagaatt cctatggaat gaaacggttt
    13621 ccgggaccat ggtgcttcag ggaaggatgt ttaccctgca catggacgaa ttccacatct
    13681 attgcaacgg cgaaaagaaa tggtattata accagggaat tgaccaatgg gaggaaatgc
    13741 ctcacgacag caactcaccc gatatattgg aaaacccttc tgcattcttt tcccgcctca
    13801 acgaggatta ttaccatccg gacactccgg tgaagaagac aagcatggca ggagaccctg
    13861 tatgggagct gactttagtc ccgaattcca ttgaatctcc ctttacttat gtaattctgg
    13921 gcattaccgt tgaggggctc aaacccttgt acatccgcta tgccctgacc gacggaacag
    13981 catacccggt aaaaatcaac tcttttaaaa ccgtttctgc ccggccggaa tcattctttt
    14041 tgcccggatt ctcccgctga cacccccagg aggtgttgtt gcaggaaacg tgtcactttt
    14101 tcaaaggcat ccgtcgtcag ggtggatttt tttaacatga aacagtgcct gccgaaggga
    14161 taatccagca attgcgcatc cacgccattt tctgtcaacc ggttgtaata ccttacgcct
    14221 tcatcatgca gggaatccct tccggcaacg attatcaatg ccggaggcag tcctttcaga
    14281 tcctcatctt gcgcataaac aggtgaaata ttaggatcgt gaccgtcatt gccggcaagg
    14341 tagctactgt cgaacatgta agcaaacttt gggggtatgc agcctttggg taatggtttt
    14401 tcaatagctg aagtggccat gtccaacgga ggacaattca taaactggca taccggttga
    14461 atttttcctt cttttttcgc tttcatgcag gtaacagcag tcagattggc accagcacta
    14521 tgacctccaa tggcaatcct tgctttatct atgccataga cctttgcatg ctcccagagg
    14581 tattccagaa tggcatagac ctgatccacg gctgctggaa agggatgacc gggtgctttg
    14641 ggataatcta tgctgatgat cctgcatccc agaagacggc tccattcgat attcattctt
    14701 tcgtccattt cggggcttcc aagaataaat cctcccccgt gcaggtcaat aagcagcgga
    14761 ccgggagatg ggttttcaaa tccataccat actgtccggg tatgcccatg ccgggtttcc
    14821 ataaacactt ctttccgcag tgctttgttc ctttttatca tggcatggta cattgccctg
    14881 gaggccattt tctccatttt ttgaagacgc atgcctttct tctggtattg ttccggtgtg
    14941 agaattttca tgagttattg tattgaaagg tgaatcctgc gggaaacagg atgcaggttg
    15001 tttgaacgct tacccaggtt cttgacaaag ccaaggggaa ggccattgta tatgagcagc
    15061 aggtatcctt tttctgttcc ctgcggtaac acaaggggat tcttgctgag gaaagccagc
    15121 gcctgctccc ggtcaatgac tacaacagga taggcgtctt ttttcagatc tgttgctagg
    15181 gccaggtctg cccacgggac ccagtcatct cccttttttg tcgcgatggc aatcccagag
    15241 tgaaacacat ggagcatgga tccaagggcc tccatttccc ggtgcgattc ccggggaaag
    15301 gccttgagca tttgtccttt catggaccag gaataggagc cttccagatg tagtggtgct
    15361 ctttccggaa cgcggtaagg agtccaccct tttttcccat caggtgcagg caggattttt
    15421 tttaccccgg cttcccgtgg tccgttcttt tccagcagtg ccatgaaaaa tccttcaccc
    15481 ctgacacgat ggggcaggta tttttgcggg ccgagcagca ggcgggcacc caggtgatcc
    15541 cttatccagt caatctgttc ctcatcttcc atgcggttaa gggtgcagct gctgtataga
    15601 aaaagacctc cccggcgcag ggaggaccat aaatcgctca gaatccttct ctgacgggcg
    15661 acacataatg ccacaagccc gggcgaccag gttttgcggg tatcgggatc tttacggaac
    15721 aacccttctc cggaacaggg gacatcggct accaccacat caaaatattc aggtaattta
    15781 ccaaagtcag ccggatcgtt ctgggttaca acaacattgg cgtatcccca tttggccagg
    15841 ttttcggcta gaatgggaac ccgggtgcgg atgacctcgt tgctcaccag gagtccctct
    15901 ccatgcatca ggtcaagcag caatgtgctc ttgccaccgg gagcagcaca ggcatccagc
    15961 acccgtggtg aggttatgtt gtccagatag ggtccaagct gccagagaga catacccgaa
    16021 gcttcctgta catagtaagc cccggcatga aaacgggggt ccagtgtgaa tacaggcctt
    16081 tcgtccaggt acctgccgag gggacaccag ggaaccgtat ccgttccggg aaacaattcc
    16141 ccggccgctt tcagtgcatg ttgtcttatg ctcactgtcg gttggctgtc aaggcaggac
    16201 aaaagaggat ccacatcacc tggcataagg gatgcttcca tcatatgtct gaattccgtc
    16261 attggatttt ccgctttccg gatgtcactt attcaggcct tctgccaatt gcgttgtgaa
    16321 ttgattgaca aaatccttga tttttcctcc aagcatcatt ttaagcatca tattgagttc
    16381 ggcatccagg aaaagctgca gttcacaacc ttcttcccgg ggccggatat gaaggttcag
    16441 gttgaatgca aaggggacgt tgccatgctg ttccataaca ataagggaag gctcttccct
    16501 ttttgtgact ttgactccaa tttgcatccc ctgggcctcg gccaggatgg tatcttttgt
    16561 tattatcacc ttgtctttct tgtcttcagg caagcgttcc gaaaatcttg aaaaatcgga
    16621 gaaaagatga taaaccaccg atgccggttt gtttacaaga caaggatttc cctcaatgtg
    16681 tgtgtttccc atagttagcg tccccaggtg ttcggcgtca ggcgccagat tttaagtgtt
    16741 tcgagttgtt ccggactaac gaaattcctt tctgcagctt cctgtataag cgtattgtaa
    16801 ttggtaagcg tatgcagctc gcagttgctt ttttcgaagg ccctccgggc aacatcaaaa
    16861 ttgtaggaaa aagtggccac aacgcccaat acgttagcgc atgccatcct aaggcaatct
    16921 gccgcagaaa gtgcagagtg acccgtagaa ataaggtctt ccaccaccaa cacattagaa
    16981 ccgggtgtca gggtcccttc aatgagggca cctgttccgt ggtcttttct gcggggacga
    17041 acgtagatga agggtttacc cagccggtcg gctataaggg cgcccattgc aatagcccct
    17101 gttgccactc cggctatgac atctgcagtg gggtagttct gttcaatgga cctgcaaagg
    17161 gcatcacata tgaagtttcg taccgtcggt acagataaga cttttctgtt atcacaatat
    17221 atgggagaca accaaccgga agcccattca aaaggccttt caatgttgag ttgcactgct
    17281 tcaatttcca gcaggatttg tgcaactttt ttttcaacag attccataat aatcacatat
    17341 ctttgtgcaa atgtacagta ttttcttcaa taatagacga cttcagttat gccgtccgga
    17401 caacccggtt tgtcttcttc cttcagttac ggtagaatct tttgaaaacc actcttcctg
    17461 ggaagaattg attgtcagtt tccaatgcaa cacaagcctg agacacgtag tggtgccggt
    17521 tcaaaatccc cacgattcct ttatgcagat atgtaaaaat ttcaccatta tgccggctgc
    17581 aggggggctt gtcaccaaca aacaagacga cttgctgatg atctaccgtt acggtaaatg
    17641 ggatttaccc aagggaaagc aggaacaagg ggagaaactg agcgataccg cactcaggga
    17701 agtggcagaa gaaaccggga ccattgacct gaaactcttg ggagaccaat tgaccacaac
    17761 acttcactgt tatcattata agggaaacaa tgtgctcaag atgacggcct ggttccccat
    17821 gacagccaat tccggaacaa ccttactgcc acaggcggaa gaaggaattg aaaaagcgga
    17881 atgggtttcc cgcaaagacc tggcagagcg tctggatatg tcctatttat ctataaagat
    17941 actggttgaa aaacagtttc taaacgattg taaaaatgtt ggtgaagcct gtaagatcga
    18001 agatatcctt gatctctgcc cgtagatttt taaccgtcag ggttccgcct ttttctttta
    18061 tgtttttctg aagaaccaga aattgacgca gacctgagct gctgatatat tccagaccgg
    18121 ttccgtctat gatcagttcc ctggtgttgc ttttaagaat ggggacgata gcttcttcaa
    18181 agatggcggt attgcctgta tccattctcc cgtttattga gattacggga acgggatcgt
    18241 tgctgattat tacttccata tactgttgtt tttctttaca gagattgatt gtcaacaaag
    18301 gtaattaaaa aactgttcat ccgccaaagt catcaaacag aatatgatcc gcaggaacgc
    18361 ctagattgtc gagcatttcg gtcacggccg atgtcataag gggcggtccg cagatgtaaa
    18421 attctgcctc ttccggttcc ggatggtctt tcaggtattg atcgtatagt accttgtgga
    18481 taaagcccgt atggccggtc caatgatcct gtggcctggg ttctgataga gctatgtgaa
    18541 atgaaaaatt ggggaattct tttgctatcc ggaggaactc atcttcataa aaaatctccc
    18601 gcctggaacg ggccccgtac cagaaagaaa ccttatctgt tgcccggagg gttttaaata
    18661 agtgaaagat atgggatcgc atgggtgcca tgccggcccc gcctccaatg aatatttttt
    18721 cggcttgtgt ttctttgagg aagaattccc cgaagggtcc tgaaatcatt actttatccc
    18781 cgggcttcct ggaaaaaata taagaagaac aaataccggg attgaccttc ataaacgttc
    18841 cccttgagcg gtcccagggt ggggtggcta tgcggacgtt cagcatgata cggttccctt
    18901 cggccgggtg gttggccatg gaataagccc tgaagctgta ttctttgttt accattttca
    18961 gtccccagat acccagtgag ctccagtctt cccggaatag ttcatctata gcgatgtctt
    19021 tggaaaagtc cacagtgacc ggggggacat caatctgtac ataacctccg gagcggaaat
    19081 ccagtttttc gcccggtgga agctgaatca caaactcttt gatgaaagag gccacgttgc
    19141 ggttgctgat cacttcgcat tcccattttt ttacacccag cacctgggca gggatgtgga
    19201 tttccatgtt ttcccggacc ctgacctgac atccaagccg ccattggctt tgctgttcgc
    19261 gccgggtaaa aaagcccgtt tctgtggaca gtatggaccc ccctcctgac agaacgcggc
    19321 atttgcagag cccgcaggtt ccgctgccgc cgcaggccga gggtaggtat attttgtttt
    19381 gtgccagggt ggtcagcagg ttgttccccg gttcaacctc cagcattttt tcgtcattga
    19441 ccttaatttt cactttaccc ccgggtgtca gcctggcttt tgcatagatc aaaacaacaa
    19501 ccaggaccag ggttacggtc agtatgacgg caaaagtcag gaacagaaat tggaaaatgg
    19561 tcattggttg tatttaagtt tgtaatccca taaaactcat aaaggcaata cccataagcc
    19621 cggtaatgat gaaagcaatg cccaggccac gcatgggtgc cggaatgttg gaataactca
    19681 gtttttcccg gatagcggca atggagacaa tggccaggta ccacccgatc cctgatccca
    19741 gggcatagga aaagacttcc aggatgttgc cgtattccct ttcctgcatg aacagcgacg
    19801 ctcccagaat ggcacagttc acggctatca gcggcaggaa tattcccaga ttgttgtaca
    19861 gggcgggtgc aaatttttca actaccatct ctaccaattg taccatggcg gctatcacgg
    19921 ctataaagag gataaaactc aaaaaaccca gatccacctc tgcccattgg ggattgatcc
    19981 aggccagggc tcccggggag agcacgtaac ggtccagcag gtaatttacc gggagggtga
    20041 tgagcagcac aaagatcacg gccaggccca gtccagacgc ggttttcacc gttttggaca
    20101 cggccaggta ggaacacatg cccaggaaat aggcaaagat catgttctct gtgaagatgc
    20161 ttttgacaaa tatattggct atctgttcca tacgctatac cctctttgcg tttttggcat
    20221 tgaagaccca gactataagt cccagtatga taagggccat gggaggcagg ataaagaggc
    20281 cgttgttttc atagaaaccc ccgtttgcaa cataccactg ctgtgggatt acctgataac
    20341 ctatcaaggt tccggatccc aggagttccc ggacaatgga tacggcaatg agtatgatcc
    20401 cgtaacccag accattggcc agcccatcca ctaatgaggg aaacggcttg ttatagaggg
    20461 caaaagcttc tatccggccc atgatgatac agttggtaat gatcagtccc acaaaaacgg
    20521 aaagctgctt gcttatctcc catgcaaagg ctttcagaat ctggtcgacc aaaatgacca
    20581 gtgcggccac caccaccagc tgtactatga tcctgatgcg gttgggaatg gtattgcgca
    20641 gcagggaaag aatcatagaa gagaacccgg tcaccagggt cactgaaagg gccatgacca
    20701 tggccggttc cagctttaca gtcacagcca gtgcagaaca gattcccagg gccagtaccg
    20761 gaatggggtt gttcttgaaa agcgggtcca ggaatatgcg tttattcata atgtttcaaa
    20821 aaaggtaaat agggtttcag ggattcccgt atcatattct caacaccacg actggtgagc
    20881 gttccgcctg aaataccgtc aacagcattg ggatt
//
LOCUS       DAOSOF010000014        20841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2545_length_20841_cov_4.751034, whole genome shotgun sequence.
ACCESSION   DAOSOF010000014 DAOSOF010000000
VERSION     DAOSOF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20841
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_20841_cov_4.751034"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="tsf"
                     /locus_tag="PLM68_01630"
     CDS             complement(<1..804)
                     /gene="tsf"
                     /locus_tag="PLM68_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT54105.1"
                     /translation="MEIKAADVAKLRKMTGAGMMDCKNALVESNGDFERAQEIIREKG
                     KLVAAKRADRETTEGSVIAITNEKKDRGILVCVGCETDFVAKNKEFLDLAMGIARKAL
                     EHFPENLEALLNLPYQESTIADAVTQQTGKSGEKHVVVFYSRLESPYMGSYIHMNNKV
                     ATIVGFSKPIPAKAAKEIAMQITAMVPVAVNRESCPKEIIEKELHIYREQIRMEGKPE
                     EMVEKIALGKLNKFFKDYTLEDQIFVKDGKISVKEYLKTVDPQVRVTAFS"
     gene            complement(819..1724)
                     /gene="rpsB"
                     /locus_tag="PLM68_01635"
     CDS             complement(819..1724)
                     /gene="rpsB"
                     /locus_tag="PLM68_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT54106.1"
                     /translation="MPRTNFQELLDAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTIVKIEEAASVAKQLAKAGRKILFVATKKQAKDIVAEAAKEINMPYVTERWPGGMLT
                     NFPTIRKAVKKMNTIDKMMIDGTFETLAKRERLQVTRQRAKLEKNLGSIADLNRLPAA
                     VFVVDIQKEVNAVKEATRLNIPVIAMVDTCCDPTPIDFVIPANDDAAKSIALVINIIK
                     QAINEGLEERKMEKDKDVTLDLSEEEDSDVPVKRTRSRRQRIDEDRKEEAPAKSRQME
                     SVKEEAAPVKEEEVPAVEIKENKPE"
     assembly_gap    1820..1919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1965..2417)
                     /gene="rplM"
                     /locus_tag="PLM68_01640"
     CDS             complement(1965..2417)
                     /gene="rplM"
                     /locus_tag="PLM68_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT54107.1"
                     /translation="MNTLSYKTVSANKASVTKEWVVLDATDQVLGRLAVKAATLLRGK
                     HKPNFTPHVDCGDNVIIINADKVRLTGNKLDEKTYVRHSGYPGGQRSETAKELLKRKP
                     LALVEQAIKGMLPKTRLGSELFRNLYVYQGPEHPHQAQQPKQITLNEN"
     gene            complement(2503..3483)
                     /gene="pfkA"
                     /locus_tag="PLM68_01645"
     CDS             complement(2503..3483)
                     /gene="pfkA"
                     /locus_tag="PLM68_01645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT54108.1"
                     /translation="MKNIKTIGVLTSGGDAPGMNAAIRAVVRTSTYHGCSVVGIQRGF
                     TGLLQNLFIPLQNHSVAQKISLGGTFLKSSRCPEFKSAETRSVAWQNMQQQGIDSLVV
                     IGGDGSFRGAELLVNEFGLPVVGIPGTIDNDIYGTDYTIGFDTAVNTAVQAVDKLRDT
                     ADSHNMVFFVEVMGRDAGFIALSTSISTGAEATLIPELQTDIDKLCEYLELERRKNKT
                     SGIIIVAEGHEQGSATQVAAKVKERLPAYETRVTTLGHVQRGGSPTANDRILASILGH
                     DAVVALLEGKTGVMVGQVNNQTVFTPFQEAYSRHSAINKRWVDIAYILSL"
     gene            3519..4493
                     /gene="dusB"
                     /locus_tag="PLM68_01650"
     CDS             3519..4493
                     /gene="dusB"
                     /locus_tag="PLM68_01650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT54109.1"
                     /translation="MKIATIDLGKEPLFLAPMEDVTDASFRFVCKEYGADMMYTEFVS
                     ADGLIRDAYKSRDKLLISDSERPIGIQIYGHVPDVLLQAARIAEEAAPDVIDINFGCP
                     KHKIARRGAGCGWLRDPEGMVDITRRLVQSVKVPVTVKTRLGWDHESVIIEDLAEALQ
                     DAGIAALTIHGRTGTQMYKGEADWAPIGAVKANPRMHIPVIGNGDICTPLQAREAFDR
                     YGVDGIMIGRASFGHPWLFREIRHYLQTGELLPPMGVSERVALAKRHLARSLEVKGER
                     VGVLEMRRHLNSYFKGLSDFKPVRLQLVTENDPQALFGILDTIAEKWG"
     gene            4490..5482
                     /gene="lpxK"
                     /locus_tag="PLM68_01655"
     CDS             4490..5482
                     /gene="lpxK"
                     /locus_tag="PLM68_01655"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOT54110.1"
                     /translation="MTGSYLQWLSPYAWVLALRHLLYDRGLFRSYAFDVPVICVGNIT
                     AGGTGKTPMTEYLAGRLLEKGKQPLVLSRGYGRKTRGFRYVELNASSMETGDEPLQIK
                     RQVPGAVVAVCEDRAEGIRRLVVDHPHDPWIILDDAFQHRRIRAGKNILMSDHARPFA
                     RDILLPFGRLRDLPSAAGRADCIVYTHCPGSMAGCEKIGDRHTGIPVFYTFMSYGSLL
                     PLHNPRTLPHAGSPVLLVTGIANARALKEHVQQHFLLARHLEFPDHHRFTGKDIARML
                     ELLDRNPSWQLITTAKDAVRLAGSGIPGWVIPVRISFFPGESEDAFFHLVTGLR"
     gene            complement(5406..7730)
                     /locus_tag="PLM68_01660"
     CDS             complement(5406..7730)
                     /locus_tag="PLM68_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT54111.1"
                     /translation="MKNKSILLLWLFVLSGCSGDSGLQLWYDKPAEIWEATLPLGNGR
                     LGMMPDGNPLQETIVLNEITLWSGSEQDATNPAALESLPRIRALLLEGKNHLAQELMY
                     ETFVCGGEGSGRGGGAGVPFGCYQVLGNLRLEFNDKSKPEKNYRRNLDLEKAIARTGY
                     KGNRREYFTSFSHDVGVVRIKPGRKSHTVIRLDRPERFATAMENDRLVMQGVLSSGVE
                     GQDGMRYVVKVGVNHSGGRVRFEGDSIVLDGVRKATLIVSAATSYFHADPEIHCDTLL
                     EKALAVKYRHLRKKHKRAYQSLFERVYLHLGPQKNASPLPTDLRLAAYQDQPDPGLDA
                     LYYQFGRYLLISSTRPGQLPPNLQGLWANSIQTPWNGDYHLNINLQMNHWLAEPGNLA
                     ELHLPLIDFTKAIVPSGQTTARLFYGADGWTAHVICNPWHFTAPGEHPSWGATNTGGA
                     WLCQHLWTHYLYNPDKEYLQEVYPVMKGACEFFLSSMIREPSQGWLVTAPTTSPENEF
                     FMPDGITRASVCMGSTMDNQLVRELYTNTIKASEVLDLDPGFRERLREALEDLPPHKI
                     SPDGYLQEWLEDYRETEPQHRHVSHLYGLHPGNQITPETTPDLANACRATLERRGDGG
                     TGWSRAWKINFWARLGDGDRAHRLLRNLLAPESTYPNLFCAHPPFQIDGNFGGASGIT
                     EMLLQSHSGFIDLLPALPGIWSGGFFKGMKAQGNAVVSCWWKNGKVKKVILEAPDGGT
                     YLVRANDVQISVTLSPGEKKRLRFHREKKISAQE"
     gene            complement(7720..11310)
                     /locus_tag="PLM68_01665"
     CDS             complement(7720..11310)
                     /locus_tag="PLM68_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOT54112.1"
                     /translation="MKLYVSALLGLLVLVMAGCEPDTTYTKKPGNYPGDPAQDNAPVL
                     SADKENYRNIALNRMTIHSSSYDYNLTGQLVSDGIKSATPPSWINVSCHKGDLQKREK
                     EWLFDGKPDSKYRMSGEEVFIQLELSGSVPVVTSMELIGSVTYNKEKAGNYQVALYAS
                     MDGESWELIKKEQGWGFPGTEKPNPYAQWAHLLKPAAPQGPQPSFTFSYAPPASERNI
                     RQSYPFNEPVQYRFYKVAFRVPGAKDWAFSDWDFYRKSQLLVITPSIDFKSAWMSAGT
                     GREWLMVDFGAPSVIDSVALHWINKAVAGTIEVSGDKEKWDVVAQLPGTDDSVDIITL
                     DTPARGRYLKVNMESSANGKRYIMSELEAFGTGALRAVPKPRELPTPDRITLNGGDWR
                     LQRLSLVPEKGYVISRKDYQGAETWPIATVPGTVLSSYLNMGALPDPNYGDNQLQISE
                     SFFREDFWYRTEFFVPEDFKKDELFLHFDGINWVAVVFLNGERIGRINGAFTRAKFNI
                     TNKIIPGASNVLAVTIIDNRNFGAVKEQNAVSPDQNGGVLGADNPTFHASVGWDWIPT
                     IRGRNMGIWNDVFLTTTGSVSIEDPFVQTFLPLPDTTRATIRIEATLQNHKEIFLLGT
                     LEGSYGHIPFNLEVGLEPGEIRTVSTEISLENPRLWWPKGYGAPNLYPVDMTFNIGGT
                     VSDSLSFLSGVRQVDFDENNGILSLYINGRRFIGRGGNWGFSESNLNYRAREYDIAVA
                     YHADMNFTMIRNWVGQTGDDEFYEACDRHGIMVWQDFWLANPWDGPDPVSTRMFMQNA
                     NDLVRRIRNHPCIALYVGRNEGYPPKELDDALREMIPQLHPGIHYISHSSEGVVSGGG
                     PYRALPVRDYYLLYGKDRMHSERGMPNVMNYESILQTIPEEHLWPHNDMWGLHDFCLE
                     GAQGAASYLEMVEKAFGPSENAEQFSQYAQWINYDGYRGIFEGRSEFRRGMLLWMSHP
                     AWPSMVWQTYDYFFDPTAAYFGCKKASEPLHIQWNPVYHDIEVVNLHAGLHEGIRAHA
                     QIINTDGSVQWEKDTLVNSNEDSTVKCFGLEFPQTLSDVHFIKLTLYGNDRILSENFY
                     WRGLEEGNLKALHNLPKVSLDSKTKREKAGDQWKLTTTLVNTSETPCLMVRLKVTGNK
                     NGERILPVFFSDNYVSLMPGESRTITMTLKNQDTRGEKPIVALSGFNYEE"
     gene            11402..13555
                     /locus_tag="PLM68_01670"
     CDS             11402..13555
                     /locus_tag="PLM68_01670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOT54113.1"
                     /translation="MKKTFPGTAAFLAGAVVLITSCGRSFTQYVNPLIGSGGHGHVFV
                     GAGVPFGMVQAGPVQYNTGWDWCSGYHYSDSTIIGFAQMHLSGTGIGDLGDISLMPVA
                     GDVETNRKGLVSRFSHDNETVRPGYYSVLLDGSGIRAEMTSTSRVAFHRYTFPDDDRR
                     KCVVIDLEHGIGWDSPAEGHLQWDGGRVVKGYRISTGWARHQEIFFYAEFSVPILEAE
                     FFEESRPSGGNSITARKAYAKVWLDVPRNGEVLVKVALSPISMEKAAENMEHELPWWN
                     FRAVIRAADKAWNRELSKINYITDNPDDKVVFYTALYHTMIAPSLFCDAGLPDGFARY
                     TTLSLWDTYRAAHPLATIIHPEKMGDYVRTMLDIYRQQGKLPVWHLVGNETDCMVGNP
                     GIPVLADMVLKGYAKDPLQALEAMKESALREERGMDLYREYGYIPFDMDPGHETVAKT
                     LEYALADWCVAQVARMAGDTLTENEYTRRSKAYEHLFHPLSGFMRARSASGEFREPFS
                     PFRSSHREDDYTEGNAWQYTWLVPHDIPGLITLFGGPEPFVNKLDSLFLAEGDLGEEA
                     SPDITGLIGQYAHGNEPGHHIPYMYAFAGQPDKTAKRVRQILTTMYHNAPDGLCGNED
                     AGQMSAWYILSALGFYQVEPAGGRYVFGSPLMDKAVIRVRDGKTFTIVAHDNGPENHL
                     IRNIRLNGRPYDKFYIDYRDIMAGGLLEFNMCSAL"
     gene            complement(13838..16078)
                     /gene="pnp"
                     /locus_tag="PLM68_01675"
     CDS             complement(13838..16078)
                     /gene="pnp"
                     /locus_tag="PLM68_01675"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT54114.1"
                     /translation="MNIINKVITLDDGRKIEIETGKLAKQADGSVVVKMGGTMLLAAV
                     TCAKEAKPEADFMPLSVDYKEKYAAAGRYPGGFLKREGRPSDYEILVSRLIDRALRPL
                     FPDDFHAEVFVNVFLISAEKQIMPDALAGLAASAALAVSDIPFNGPISEVRVGRINGE
                     FIINPSFSQLEEADLDIMVAATYDNIMMVEGEMKEVSEAVMLDAIKFAHQEIKKHCKV
                     QMELMEEAGKTVKRAYNHEDNDEEIRKAVETFCYDRCYAIARSGEDKHTRSDAFEALK
                     EEFMQTIPEEEREEKALMISRYYHDVEKRAMRRMILDEGIRLDGRSTTDIRPIWCEVD
                     YLPMAHGSAIFTRGETQSLTTVTLGTKLDMKEMDEVLIQDTEQFVLHYNFPPFSTGEA
                     KPSRGTGRREIGHGNLALRALKAMVPVGEANPYATRVVSDILESNGSSSMATVCAGTL
                     ALMDAGVKIKKPVSGIAMGLITDQGNKKYAILSDILGDEDHLGDMDFKVTGTRDGITA
                     TQMDIKVDGLPYEVLEQALEQARLGRLHILNEMMKTISEPRADYKPHVPRIVQITIPN
                     TFIGAVIGTGGKVIQEIQKKTGTTITITEEGEFGIIDIFGENKEGMDAALAIIKGITT
                     VPEVGEVYTGKVVSILEFGAFVEILPGKEGLLHVSELDWKKIDKVEDVVKVGEEVTVK
                     LLEVDEKNGKMRLSRKALIEKPEGYVEPERKPRPSGGDRDRNSRGFSRGNDRGNSRGR
                     GGDRHR"
     gene            complement(16278..16553)
                     /gene="rpsO"
                     /locus_tag="PLM68_01680"
     CDS             complement(16278..16553)
                     /gene="rpsO"
                     /locus_tag="PLM68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT54115.1"
                     /translation="MAEYLNAQKKQEIFGQYGTSNKDTGSAESQIALFSYRIAHLTEH
                     MKSNRKDYGTQRALLRLVGKRRRLLDYLKANDIERYRNILKTLNLRK"
     gene            complement(16644..20789)
                     /locus_tag="PLM68_01685"
     CDS             complement(16644..20789)
                     /locus_tag="PLM68_01685"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOT54116.1"
                     /translation="MQAPPAGSYRIRVKKGRTASETAFYSGTVASMFRSFRNTGYVYA
                     ADLNTGEPFTKAEVRFVKDARPSFSIDMDGFTPVDVRTGESFRLSPPAPLRAVPDTFA
                     VPLTVHPWDFSYGYPFDKSTSAAIFTDRKLYRMQDTLFFKGIVTRYSNRRCEPETGKR
                     YTVVLRNNASYRDTLYTVEVVTNQYGSFSGYIPPGIVRLNGEHSLTMPGASALIRVEE
                     YTRPSFSIDLLPVKQAYAYGDTIIQDGVVGNYAGFPLDGVAVICRVYRQPMFRPLYRD
                     PHLYPAAGEPCSTDTLLTGSDGWFRAAIPAGKNDEDNPRGEWLQVHFSATDPSGETWQ
                     QQTFLPVSNHRYTVHTRLGSAGTEARDRILVKEKGPFLALEVKNSTGHDQEVSGTYTL
                     SRNRVIQYSGTFTGKETSLPSWSAMESGQWELSLDLAGAPPHKETFWLVSMQDTVSPA
                     DTAAFFCPLDTTDPSFLLGTVDRQLFALAEWYVADSLLKKYHIVLQPGMRTFSFGEAV
                     HESYPLELRLIAIRDGIFHEFRHRWEEPPGLEPAIQFVSLRKVAGPYSRETFGLKLPA
                     MEKAEVLVSIFNKSTDRFQPNSFSYQPARAPYVSIPYIRHHFMQTTTGYAGGTKGPVM
                     YRATSPYMVQGNNKAAGEEMAQARTDDSHENAMAEALSPAQQEETPVVRSDFEETLAF
                     LPHLVPDSTGYVPVAFRTNGLLSTFRILVLAHTSDGKSIPADEILTVKKEVMALPYFP
                     TYVRSGDSISLTWRVVNLTPDTLQGKAYLKAGDKDKACQPATLLPSARLLFRQDFQAP
                     RVRTLHVPLLTLTAGFESPSLSDAETHTIPVLSMMEQVTRAQTKTLQGNGVVTLLKKD
                     KSSEANLEVTTPLSSALQALPVLCEPESNNLTSWVAAFYANNTGARILERFPHILDTL
                     RAHPSGQAAVFEKSNRTGALLLEETPWSGYPGQEAERMQRLLRLADPAYVRSFNARAL
                     EHFRALQRQDGGFSWFPGMESSYLLTLHFLEKIGDMIEKEVVAYDDEPLYSIVRKALR
                     YTDSLFINNTDKEVIRNLGTDVNLYFFVRSAFRQKFPLGPDIIRIAARLARNPLQSWK
                     GASVMEKAYLAVTLERWGETKALESLLASLREFAVCDSQMGCHFPNAVPYEGPMSSQM
                     KAHALLLRIFNTDPILREGITRWILDRKENNIWTSRAGTTDVIHALLYYGGSVSSSPA
                     RYEILQRGTTYTVRSRTDALLYVSLYEHTTEDFSAAAPYSSGLEINRTWHRAADNAPV
                     GEGDTLRPGERILARYHLNNDKDRSFVHLKASRASCLMPESETSGYQGNISCSWFREV
                     KEASTQHFFQNLPAGTHVIEEGFYVTREGVFNQGSIRVQSLYAPQYGGFSPGGKLFVK
                     EKR"
BASE COUNT         4575 a         5328 c         5495 g         5343 t
ORIGIN      
        1 cgaaaatgcg gttaccctta cctggggatc tactgttttc aggtattcct taacagagat
       61 ctttccgtcc ttgacaaaga tctggtcttc cagcgtataa tccttgaaga atttattgag
      121 tttgcccagg gcaatctttt ctaccatttc ttccggcttg ccttccatac ggatctgctc
      181 acggtaaatg tgcagttcct tttctatgat ttctttgggg cagctttcac ggttgacagc
      241 taccgggacc atggcagtga tctgcattgc gatttctttg gcagcttttg cgggtatggg
      301 tttgctaaag cccacgatcg tggctacttt attgttcata tgaatgtatg agcccatgta
      361 gggagattcc agcctgctat aaaaaacgac aacatgcttt tccccgcttt ttccggtttg
      421 ttgggttaca gcatcggcaa tggtgctttc ctggtaaggg aggttgagca gggcttccag
      481 attttctgga aaatgttcca gggctttgcg tgcaataccc atggcaagat ccagaaattc
      541 tttgttcttg gctacaaaat cagtttcaca acccacacag accaggatcc cgcggtcttt
      601 cttctcattg gttatggcta tcaccgatcc ttcggttgtt tcacgatctg cccgcttggc
      661 ggcaaccaat ttcccttttt ctctgataat ttcctgtgca cgttcaaaat ctccattgga
      721 ttcaacaaga gcgttcttgc aatccatcat gccggcaccg gtcattttgc gtagctttgc
      781 tacgtcagct gctttgattt ccatatatat gatttttctt attcgggttt attttctttt
      841 atttccactg caggaacctc ttcttccttc acgggagctg cttcttcctt cacggattcc
      901 atctgcctgg atttagcggg agcttcttcc ttcctgtctt catcgatacg ttgcctgcgg
      961 ctgcgtgtac gctttacagg aacgtcactg tcttcttctt ccgataagtc aagggtaaca
     1021 tccttgtcct tttccatttt gcgctcttcc agtccctcgt ttatggcctg tttgataatg
     1081 ttaatgacaa gtgcaatgga tttggcagca tcatcgtttg ccggaatcac aaaatcaatg
     1141 ggtgtaggat cgcaacatgt atcaaccatg gcgataaccg gaatattcaa acgggtagcc
     1201 tctttgactg cgtttacctc tttctggata tccactacaa aaacagcagc aggtaaacgg
     1261 ttcagatcag cgatagaccc gaggttcttt tccagtttgg cacgctggcg ggtaacctgc
     1321 aggcgttcac gctttgcaag agtttcaaaa gtgccgtcta tcatcatttt atcaatggtg
     1381 ttcatttttt tgacggcttt gcgaatggtg ggaaagttgg tgagcattcc gcccggccag
     1441 cgttccgtga cataaggcat gttaatctct ttggcggctt ctgccactat gtctttggct
     1501 tgtttcttgg tggcgacaaa caggattttc ctgccggctt tggccagttg ttttgcaaca
     1561 ctggcagctt cttctatttt aacgatagtt ttgtgcaggt caatgatatg gatcccgttt
     1621 ttctccatga atatataagg ggccatttta ggattccact tacgtttgag gtggccaaaa
     1681 tgtactcctg catcaagaag ttcctggaaa tttgttcttg gcattgttat ggtttttaaa
     1741 aattagtttt tttctctttg tagcaattaa agggtagtgt cgctgacaat ccgctttaat
     1801 tttccgcggc aaccgaaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1921 ctgattttct tcttccaagg gcgttgatga cttccatgct ctgtttagtt ttcgtttaaa
     1981 gtgatttgtt tgggttgctg cgcctggtga ggatgttccg gaccctgata tacatacagg
     2041 ttgcggaaaa gctcagagcc gaggcgagtt ttggggagca tgccttttat ggcttgttcc
     2101 acaagggcaa gcggtttacg cttgagcagc tctttggctg tttcactgcg ttgaccgcca
     2161 gggtatccgg agtgccggac atacgttttc tcgtccagtt tgttcccggt caggcggacc
     2221 ttgtctgcat tgatgataat gacgttatca ccacagtcca cgtggggggt aaaattgggt
     2281 ttgtgcttgc cccgcaacaa agtggctgct ttgacggcaa gacgtcccaa cacctgatcg
     2341 gtagcgtcca ataccaccca ctctttggta acagaggctt tattggcgct tacggtcttg
     2401 taggataatg tgttcacctg gttgattttt agaataattg tataataaaa ttgcaatccc
     2461 tgaatttaaa ggggctgcaa agatactact tttttccaga aatcaaagcg ataatatgta
     2521 agcaatatcc acccagcgtt tgtttattgc actatgccgt gaataagctt cctggaaagg
     2581 agtgaacacc gtctggttgt tgacctgccc gaccatcacc cctgtttttc cttccagcag
     2641 ggcgactacg gcatcatgtc ccaggatgct ggccagtatg cggtcgttgg cagtggggga
     2701 gccgcccctt tgcacatggc ccagcgtggt gacccgggtt tcgtatgcgg gaagccgttc
     2761 tttgactttg gccgctacct gggttgcact gccctgctca tgaccttctg ccacgatgat
     2821 aataccggaa gtcttgtttt tacggcgttc cagttccagg tattcacaga gtttgtctat
     2881 gtctgtctga agctcgggga taagggttgc ttctgccccg gtcgaaatgg aggtgctcag
     2941 tgctatgaat cccgcatccc ggcccattac ctcgacaaag aacaccatgt tgtgcgagtc
     3001 tgccgtgtcg cgtaatttat ccacggcctg tacggctgtg ttgactgcgg tatcaaatcc
     3061 tatggtgtaa tcggtgccat atatgtcgtt gtctatggta ccggggatcc ctacaacagg
     3121 gagtccaaat tcattgacaa gcagctcggc acccctgaag gaaccatctc cgcctatgac
     3181 gaccagggaa tcgattccct gttgctgcat gttctgccat gcgacagaac gtgtttctgc
     3241 ggatttgaac tcgggacagc gtgaagattt aaggaaagtg ccccccagtg atatcttctg
     3301 ggcaactgaa tggttttgca gcggtatgaa caggttttgc aaaaggccgg tgaatccgcg
     3361 ttgtatcccg acaacactgc atccgtgata tgtggatgtc cttacaacgg cccttatggc
     3421 ggcattcatt ccgggagcgt ctcctccgga tgtgaggact cctattgttt tgatattttt
     3481 catacgactg caaaagtaga aaaaattatc tttgtcatat gaagatcgca acaattgacc
     3541 tggggaaaga accgcttttc cttgccccga tggaagatgt gacagatgct tcgttccgtt
     3601 ttgtgtgcaa ggaatacggg gcagacatga tgtataccga atttgtttcg gcagacggcc
     3661 tgatacgtga tgcatacaaa agccgtgaca aactgttgat atcggacagt gaaagaccca
     3721 tagggataca aatatacgga cacgttccgg atgtgttgct acaggctgcc cgcattgcag
     3781 aggaagcagc cccggatgtg atagatatca attttggctg ccccaagcac aagatcgccc
     3841 ggcggggggc gggctgcggg tggctccgcg atcctgaagg catggtggat attaccaggc
     3901 gcctggtgca gtcggtgaaa gtccccgtaa cggtcaagac ccgcctgggt tgggaccatg
     3961 aatcggtcat cattgaagac cttgccgagg ctttgcagga cgcaggcatc gccgccctca
     4021 ccattcatgg aaggactggc acgcagatgt acaaggggga ggcggactgg gcacccattg
     4081 gcgccgtgaa ggccaatcca cgcatgcaca tacccgtcat cgggaacggg gatatctgca
     4141 cgcccctgca ggcccgggag gcttttgaca ggtatggtgt ggatggcatc atgattggcc
     4201 gggccagttt cgggcatccc tggctattcc gggagatccg ccattatctg cagaccggcg
     4261 aactgcttcc gccaatgggc gtgagcgagc gcgtggcact ggctaaacgg catcttgcca
     4321 ggtctctgga agtgaaaggg gaaagggtgg gagtgctgga aatgcggcgc catctgaaca
     4381 gttatttcaa aggcctgtcc gatttcaaac cggtccggtt gcagctggta acggaaaacg
     4441 atccgcaggc gttgttcggc atcctggata ctattgcaga aaaatgggga tgacaggatc
     4501 ataccttcaa tggctttcac cctatgcatg ggtattggcc ctgagacacc tgctttacga
     4561 tagggggctc ttccggtcct atgcctttga tgtccctgtc atctgtgtgg gcaatatcac
     4621 cgctggaggg acgggcaaga cacccatgac agaatacctt gcagggaggt tgctggaaaa
     4681 gggcaaacag cccctggtcc tgtccagggg ctatggccgt aaaacccgtg gattccgtta
     4741 tgtggaacta aacgcgtcca gcatggaaac aggtgatgag cccctacaga tcaaaagaca
     4801 ggttcccggg gcggtagtgg ccgtttgtga agaccgggca gaggggatca ggcgcctggt
     4861 ggtagatcat ccgcacgacc cctggatcat cctggatgat gcattccagc acaggcggat
     4921 acgggccggg aaaaacatac tcatgtcgga tcatgcaagg ccttttgcac gggatatcct
     4981 gctgcccttt ggccggttac gggacctgcc gtctgcagca ggcagggcag attgcatcgt
     5041 ctatacacac tgtccgggat ccatggccgg atgtgaaaaa atcggggacc ggcacacagg
     5101 tattcctgtt ttctacacct ttatgtcgta tgggtcgctg cttcccctgc acaatccccg
     5161 gaccttgccg catgcaggtt ctcctgtgct gctggtgaca ggcattgcca atgcccgagc
     5221 gctgaaggag catgtacagc aacactttct cctggcacgc catctggaat ttccggatca
     5281 ccacagattt accggtaaag atattgcccg tatgcttgag ctgctagaca ggaatccgtc
     5341 ctggcaattg atcactacag ccaaggatgc cgtgcgtctg gccgggagcg ggatcccggg
     5401 atgggtcatt cctgtgcgga tatctttttt tcccggtgaa agcgaagacg ctttttttca
     5461 cctggtgaca gggttacgct gatttgcacg tcatttgccc gtaccagata ggtcccgcca
     5521 tcgggcgctt caaggatgac ttttttcact tttccgttct tccaccagca ggatacgact
     5581 gcattcccct gtgctttcat ccccttgaaa aaaccgccgg accagatccc gggcagggcc
     5641 ggcaacaggt cgatgaaacc gctgtggctt tgcagcagca tttctgtaat tcccgaggcc
     5701 cctccgaaat tgccgtctat ctggaacggc ggatgggcac agaaaagatt gggataggtg
     5761 ctttccggag ccagcaggtt cctcagcagg cgatgggccc ggtctccgtc acccaggcgg
     5821 gcccagaaat tgattttcca ggcgcgtgac cagcctgttc ccccgtcccc acgacgttcc
     5881 agggtggccc tgcaggcatt ggcaagatcg ggtgtggtct caggggtaat ctggtttccg
     5941 gggtgcagtc cgtaaagatg tgacacgtgg cggtgttgcg gctctgtctc ccggtaatct
     6001 tccagccatt cctgcaggta tccatccggg ctgatcttgt gggggggcag gtcttccagg
     6061 gcctccctga gcctttcacg gaaccccggg tcaaggtcca gcacttccga agccttgatg
     6121 gtgttggtgt acaattccct gaccagctgg ttgtccatgg tggaccccat gcaaacgcta
     6181 gctcttgtga tcccgtcggg catgaaaaat tcattttcgg gcgaagtggt gggtgcagtg
     6241 accagccatc cttgggaagg ttcgcggatc atggaggaga gaaaaaattc gcaagcccct
     6301 ttcatcaccg gatagacctc ctggaggtat tccttgtccg gattgtacag gtaatgggtc
     6361 cacaagtgct ggcacaacca ggcgcctccg gtattggtcg caccccagga aggatgttcc
     6421 ccgggggcgg tgaaatgcca gggattgcag atcacatgtg ctgtccatcc atcggctccg
     6481 taaaataacc ttgcggtggt ctgcccggaa ggcactatgg ctttggtaaa gtcaatcagc
     6541 ggcaggtgca actcggcaag gttccccggc tcggccagcc agtggttcat ttgcaggttt
     6601 atgttcaggt ggtagtcccc gttccagggt gtctggatgg agttggccca gaggccctgc
     6661 aggttggggg gaagctgtcc cggacgggta gaggagatga gcaggtaacg tccaaattga
     6721 taatagaggg catccaggcc cgggtcgggt tgatcctggt aggcggccag cctcagatcc
     6781 gtgggcaggg gtgaggcgtt tttctgtggc ccgagatgga ggtagaccct ttcgaaaaga
     6841 gactggtaag ctcttttgtg ttttttacgt aaatgcctgt atttgacggc cagggctttt
     6901 tccagcaggg tgtcgcagtg gatttcgggg tctgcatgaa aataactggt ggcagcagac
     6961 acgatcaggg ttgccttacg cacgccatca agcacaatgc tgtctccttc aaagcgtaca
     7021 cggcccccgg agtggttcac acccactttc accacgtaac gcatgccatc ctgtccttcc
     7081 accccgctgc tgaggacgcc ctgcatgaca aggcggtcgt tttccatggc tgtggcaaac
     7141 cgttccggac ggtccaggcg gatgacagta tgggattttc ttccgggttt aatcctgacc
     7201 acacccacgt catggctaaa ggaagtgaaa tattcccgcc tgtttccttt atacccggtg
     7261 cgggctatgg ctttttccag gtccaggttc ctgcggtaat tcttttcagg cttgctctta
     7321 tcattgaatt ccaaccggag atttcccagt acctggtaac agccaaaggg aacaccggcc
     7381 ccccctccgc gtcctgatcc ttccccgccg catacaaatg tttcatacat cagctcctgt
     7441 gccaggtggt tttttccttc cagcaacaat gcgcgtatgc ggggcaggga ttccagggca
     7501 gccggattgg tcgcgtcctg ctccgacccg gaccacaagg tgatctcatt gagaactatg
     7561 gtttcctgca ggggattccc gtcgggcatc atgcccaggc gcccgttgcc caggggcagg
     7621 gtggcctccc atatttcagc aggcttgtca taccacaact gcaacccgga atcaccgctg
     7681 cagcctgaaa gaacaaatag ccacaaaagc aggatggact tattcttcat aattgaatcc
     7741 ggataatgca acaataggtt tttcccccct tgtatcctgg tttttcaggg tcatggtgat
     7801 ggtcctggac tcgcctggca tcagagaaac gtaattgtcg ctgaagaaca cgggaaggat
     7861 cctttcaccg ttcttgttgc cggtcacttt caggcgcacc atcaggcaag gggtctctga
     7921 ggtgttcacc agggtggtgg tcaatttcca ctggtctccg gctttttcac gttttgtttt
     7981 gctgtccaga ctgactttgg gaaggttgtg cagggctttc aggtttcctt cctcgagccc
     8041 cctccagtaa aagttttcag aaaggatgcg atcattccca tatagagtga gctttatgaa
     8101 atgcacatcg ctcagggttt gtggaaactc caggccgaag cattttacgg tgctgtcctc
     8161 attactgttc accagcgtgt ctttttccca ttgaacggaa ccatcggtat tgattatttg
     8221 ggcatgggcc ctgatacctt catgcaatcc ggcgtgcagg ttgaccacct ctatgtcatg
     8281 gtatacaggg ttccattgaa tatgaagcgg ttccgaggct tttttgcagc caaagtaggc
     8341 tgccgtgggg tcaaagaaat aatcgtaagt ctgccatacc atggaaggcc aggccgggtg
     8401 gctcatccac aataacatgc cgcgcctgaa ctcgctcctt ccctcaaata tgcccctgta
     8461 accatcgtaa ttgatccatt gggcatattg gctgaactgt tccgcgtttt ctgaaggacc
     8521 aaaggctttt tccaccattt ccagatacga tgcggctccc tgggctcctt ccaggcagaa
     8581 atcatgcagt ccccacatat cgttgtgtgg ccacaagtgt tcctccggga tggtctgcag
     8641 gatactttcg taattcatga cgtttggcat gccccgttca ctgtgcatgc ggtctttacc
     8701 gtacagcagg taataatccc gcaccgggag ggcccggtaa ggacctcccc cgctgaccac
     8761 gccttccgag gagtgggaga tgtaatggat cccgggatgc agttgcggga tcatttcacg
     8821 cagggcatcg tccagttctt tgggaggata tccttcgttc cggcccacat atagggcaat
     8881 acaggggtgg ttccggatgc ggcggaccaa atcgttggca ttctgcatga acattcttgt
     8941 gctgacaggg tcagggccgt cccagggatt ggccagccag aagtcttgcc agaccatgat
     9001 gccgtgccgg tcgcaggctt cataaaattc atcgtcccct gtctgtccca cccagtttcg
     9061 gatcatggtg aaattcatat cggcatggta tgccacggct atatcgtatt ccctggccct
     9121 gtagttgagg ttggattcgg agaagcccca gttgccaccg cgtcctatga aacggcgccc
     9181 gtttatgtac agggaaagga tcccgttgtt ttcatcaaag tctacctgtc tcacgccgga
     9241 aaggaatgaa aggctgtcgg aaacagttcc cccgatattg aaagtcatgt caacaggata
     9301 caggttgggg gccccatacc cttttggcca ccataacctg gggttttcaa ggcttatctc
     9361 cgtggaaacg gtacggatct ctcccggctc cagccctacc tccagattga aaggaatgtg
     9421 gccgtaggaa ccttccaggg ttcccaggag aaatatttcc ttatgatttt gcaatgtggc
     9481 ttctatgcgg atggtagccc gggtggtgtc gggcaggggc aggaacgttt gcacaaaggg
     9541 atcttcaatg cttacagatc ctgttgtagt caggaacaca tcattccaga tacccatgtt
     9601 tcttccacgg atggtcggta tccagtccca gcccacagag gcatggaatg tgggattgtc
     9661 agcccccagg acgcctccgt tctggtcggg gctcactgcg ttctgctcct tgaccgcccc
     9721 aaagttcctg ttgtctatta tggtcacagc caacacattg gatgctcctg gaatgatctt
     9781 gttagtgata ttgaattttg cccgtgtaaa ggccccgttt atccttccaa tacgttcccc
     9841 gttcaggaat acaacagcta cccagttgat cccgtcaaaa tgcaggaaca gctcatcctt
     9901 cttgaaatcc tcgggaacga aaaattcggt ccggtaccag aagtcttcac ggaaaaaaga
     9961 ttcggagatc tgcagctggt tgtcaccata gttcggatcc ggaagggccc ccatgttcag
    10021 gtagcttgaa agcactgtcc ccggtacggt ggctatgggc cacgtttccg ctccctggta
    10081 atcttttcgt gaaatgacat aacctttttc cggaacaagg gacagtcgct gcaacctcca
    10141 gtcacctcca ttgagggtga ttcggtccgg ggtggggagt tccctgggtt tgggcactgc
    10201 cctgagggcc ccggttccaa aagcttccag ttcgctcatt atgtagcgtt taccgtttgc
    10261 agatgattcc atgttcactt taaggtaccg gccccgggca ggagtatcca gggtgatgat
    10321 atctacggaa tcatctgtgc ccggcaattg cgcaaccaca tcccattttt ccttatcacc
    10381 cgatacttca atggttccgg caactgcttt gttgatccaa tgcagggcca cactgtctat
    10441 gaccgaaggg gcaccgaaat cgaccatcaa ccactccctg cctgtcccgg cactcatcca
    10501 ggcacttttg aaatcaatag atggagtgat gaccaacaat tgacttttcc tataaaaatc
    10561 ccaatccgaa aaagcccaat ctttggctcc cggtacgcgg aaagccactt tataaaagcg
    10621 gtattggacc ggttcgttga atgggtatga ctgcctgatg ttgcgttccg atgccggggg
    10681 tgcataagaa aaagtgaaag agggttgcgg accctgaggg gccgcgggtt tgagcaagtg
    10741 ggcccattgg gcatagggat tcggcttttc agttcccggg aaaccccatc cctgttcttt
    10801 tttgatcagt tcccatgatt ccccgtccat ggatgcatat aaggctacct ggtaatttcc
    10861 ggccttttcc ttgttgtagg tcacagaacc gatcagttcc atggaggtga caacaggtac
    10921 gcttcctgac aactccagct gtatgaacac ttcttctccc gacatcctgt atttggaatc
    10981 cggcttaccg tcaaaaagcc actctttttc ccgtttttgc agatcaccct tatgacagct
    11041 cacattgatc cacgaggggg gtgtcgcaga ttttatcccg tcggatacca gctgtcctgt
    11101 gaggttataa tcgtaagaag aggaatgaat ggtcattcgg ttcagggcaa tgttcctgta
    11161 attctcctta tctgctgaca gtacaggagc attatcctgg gccggatcac ccgggtagtt
    11221 gcccggtttt ttggtatatg tggtgtctgg ttcacatccg gccatgacca aaaccaataa
    11281 tcccaaaagg gcagagacat ataacttcat aacgtacgat gacttgaatg acataaatta
    11341 tgtaaagtta tctaaaaatg aagaaagaca aaatacctat cttagtgaaa atattacctg
    11401 aatgaaaaaa acatttccag gaacggcggc tttcctcgca ggcgcagttg ttctaattac
    11461 ttcctgcggc agatcattca cgcaatatgt caatccattg attggcagcg gagggcacgg
    11521 tcacgtgttt gtcggcgccg gtgttccttt tggcatggtc caggccggtc ccgtgcaata
    11581 caacaccgga tgggactggt gttcaggtta ccactattcg gattccacca tcatcggctt
    11641 tgctcagatg catctgagcg gtacgggcat aggagacctg ggtgacatat ccctgatgcc
    11701 tgttgcaggg gatgtggaaa caaaccgcaa agggctggtt tcccgatttt cccatgacaa
    11761 tgaaacagtc cgcccggggt attattccgt acttctggat ggtagcggta tccgggccga
    11821 gatgacatcg acatccaggg tcgcctttca ccgctatacc ttccctgacg atgaccgacg
    11881 caagtgcgtg gtcatcgacc tggaacacgg cataggctgg gacagtccgg cagaagggca
    11941 cctgcagtgg gacgggggca gggtggtgaa aggatacagg atatcgacag gctgggcacg
    12001 tcaccaggaa atctttttct atgccgagtt ttctgtaccc atcctggaag cggaattctt
    12061 tgaagagtcg cggccttccg gcggaaacag cattactgcg cgcaaagcgt atgcaaaggt
    12121 gtggcttgac gttccccgga acggggaggt actggtcaaa gtcgccctgt cacccatatc
    12181 tatggaaaaa gcagcagaaa acatggaaca tgaactgcca tggtggaact tcagggctgt
    12241 aatcagggca gccgataagg cctggaaccg cgaattatct aaaataaact acataacgga
    12301 caatccggat gataaagttg ttttttatac agccctgtac cataccatga ttgctccgtc
    12361 cctgttctgt gatgccggcc tgccggatgg ttttgccaga tataccactc tctcgctatg
    12421 ggacacctac cgtgcggccc atccgctggc cacgatcatc catcctgaaa aaatgggtga
    12481 ttatgtccgg accatgctgg atatttaccg gcagcagggg aaattgcccg tatggcacct
    12541 tgtgggcaat gaaacggatt gcatggtggg aaacccgggt attcctgtcc ttgccgacat
    12601 ggtattgaaa gggtacgcga aagaccccct gcaggccctt gaagccatga aagaatcggc
    12661 cctcagagaa gagcgcggca tggacctgta ccgtgaatac ggttacattc cctttgatat
    12721 ggacccgggg cacgaaacgg tggctaaaac cctggagtat gccctggcag actggtgtgt
    12781 ggcgcaggtg gcccgcatgg cgggagacac cctgacggaa aatgagtata cccggcgcag
    12841 caaagcatac gaacatctgt tccatccgct cagcgggttt atgcgtgccc gttccgcatc
    12901 cggggagttc cgggaaccat tttcaccctt ccgttcatcc caccgggaag atgattatac
    12961 agagggcaac gcctggcagt acacctggct ggtgcctcac gacatccccg gcctcattac
    13021 gctttttgga ggtccggaac catttgtgaa taaactcgat tcgctgttcc tggccgaggg
    13081 agacctggga gaggaagcct ctcccgacat cacgggcctg atagggcaat acgcccacgg
    13141 aaatgaaccg ggacaccata tcccctacat gtatgccttt gccgggcagc cggacaagac
    13201 ggcaaagcgg gttcggcaga tacttaccac catgtaccac aatgcaccgg acggtctttg
    13261 cgggaacgag gatgcaggac aaatgtctgc ctggtacatt ctctcggccc ttggattcta
    13321 ccaggtggaa ccggccggcg gccgctatgt gtttggctct cccctgatgg ataaggcggt
    13381 catccgggta agggacggga aaacgttcac catagtggca catgacaacg gtccggaaaa
    13441 ccaccttatc agaaacatcc ggctgaacgg ccggccctat gacaaattct acatagatta
    13501 cagagacatc atggccggcg gcttgctgga attcaatatg tgttccgcgc tttaaggctc
    13561 ccggttgttt taatggctac agcgacgcct tacaattgca gaaacaggac gcagcgactt
    13621 gaaagaccac caggcggttg tttgagcagg agtactgctc aatggcaggg tcagctgcac
    13681 caaaaagaac ggagagcctc ggcgtaaaca aaaaagctct ccgttcttta tggtgctacg
    13741 acaacaggaa gtgagagcct tggcttatga aaaaccggct ttgcgcactt ttcatgtgca
    13801 gcaaagccgg tctggcgtta ataaccggtc agaataccta cctgtggcgg tctccgccgc
    13861 ggccccgcga attaccgcgg tcattgccac gggagaagcc ccggctgttg cggtcgcggt
    13921 cacctcctga aggacggggt ttgcgttccg gttcaacgta gccctcgggc ttttcaataa
    13981 gggcttttcg tgaaagacgc attttcccat tcttttcgtc tacttccaaa agttttaccg
    14041 tcacttcttc accgactttc acaacatcct ccaccttgtc gatttttttc cagtcgagct
    14101 cggaaacatg caacaaccct tccttgccgg gaaggatctc tacgaaagca ccgaattcca
    14161 ggatagagac aaccttcccg gtatacactt ccccgacttc gggaactgtg gtgatacctt
    14221 tgatgatggc aagggcagca tccatgcctt ccttgttttc accaaaaatg tcaatgatcc
    14281 cgaattcacc ctcttcggtt atggttatgg ttgttccggt ctttttctgg atctcctgga
    14341 taaccttgcc tcctgttccg ataacggctc caatgaaggt attggggatg gtgatctgaa
    14401 cgatgcgggg aacatggggt ttgtagtcgg cacgcggttc ggaaatagtc ttcatcatct
    14461 cgttcaggat atgtagcctg cccaggcggg cttgttccaa agcctgttcc aggacctcgt
    14521 aaggaagtcc gtctactttg atatccatct gggtggcggt gatcccgtca cgtgtgccgg
    14581 ttaccttgaa gtccatatct cccaggtggt cttcatctcc cagaatatcg gatagaatgg
    14641 catatttctt attgccctgg tctgtgataa gccccatggc aatcccggaa acaggttttt
    14701 tgatctttac accggcatcc atcagtgcca gcgtcccggc acatacggtt gccatggatg
    14761 aggaaccgtt ggattccaga atgtcagata caacgcgtgt ggcgtaagga ttggcttcgc
    14821 caaccggaac catggctttc aacgcacgga gagccaggtt cccgtgaccg atctcgcgtc
    14881 tgccagttcc acggctgggc ttggcttccc cggtagagaa aggaggaaag ttgtaatgca
    14941 ggacaaattg ttcagtatcc tggatgagta cttcgtccat ttctttcata tccagctttg
    15001 ttcccagtgt caccgtggta agagactgag tctcgccccg ggtgaagatg gcagagccgt
    15061 gggccatggg caggtaatcg acttcgcacc agatggggcg gatatcggta gtggagcgtc
    15121 catccaggcg gattccctcg tccaggatca tgcggcgcat ggcgcgtttt tccacatcgt
    15181 ggtaatagcg tgaaatcatc agggcttttt cctcgcgttc ttcttcgggt atggtttgca
    15241 tgaactcttc cttcagcgct tcaaaagcgt cggaacgcgt atgtttgtct tctccggaac
    15301 gggcaattgc atagcaacgg tcgtagcaga aggtttctac ggctttgcgg atttcctcgt
    15361 cgttatcttc gtggttgtat gcacgtttaa ccgttttacc ggcttcttcc atcagttcca
    15421 tttgcacttt gcagtgcttt ttgatttcct ggtgtgcaaa tttgatggca tccagcatca
    15481 cagcttcgct gacttccttc atttctccct ccaccatcat gatgttgtcg taagttgctg
    15541 cgaccatgat gtccaggtca gcctcttcaa gctggctgaa ggaaggatta atgatgaatt
    15601 ccccgttaat cctgcccaca cgtacttccg atatgggtcc gttgaaagga atgtcactca
    15661 ctgccagggc agccgaggct gccagtccgg caagagcatc gggcatgatc tgtttttctg
    15721 cagaaatcag aaaaacgttt acgaaaactt cggcgtggaa atcgtcaggg aacagagggc
    15781 gcagagccct gtcaatcagg cgtgatacta aaatctcgta atcggaagga cgtccttcac
    15841 gcttgagaaa acctccggga taccttccgg cagctgcata cttttctttg tagtctaccg
    15901 acaggggcat gaagtccgct tccggtttag cttcttttgc acaggtgaca gctgccagca
    15961 gcatggttcc acccattttt actaccacgg aaccatctgc ctgtttggcc agcttcccgg
    16021 tttcgatctc aatctttcgg ccatcgtcta aggtaatgac cttgttaatg atattcatgt
    16081 attttttctt ctgcaatccg ccccttccag taaggggttg ttaaaattta tccaattccg
    16141 cttatcccgg ttcccggatt gctttaattt gaaccggaat aataaagaaa gtcataaaaa
    16201 aaggctgttc cccttccgga gagtgcagcc tttgttgggt ttgcgaggga tacaacgtgt
    16261 ttccatcgcc ttgtaagcta tttccggaga ttgagcgttt taaggatatt acggtaccgc
    16321 tcaatgtcgt tggccttaag gtaatccagc aggcggcgac gtttacctac caaacggagc
    16381 agggcacgtt gtgtgccgta gtccttcctg ttggacttca tgtgttcggt aagatgggcg
    16441 atacggtaag aaaataaggc tatctggctt tcggcagatc ctgtatcctt attagacgtt
    16501 ccgtactgtc cgaaaatctc ttgctttttc tgggcattaa ggtactcggc catatgtcag
    16561 caattgattt taagtggtta ataaaaaaat gacccccaaa cggggcagca aagatatgca
    16621 aattttccaa taattacttt tttttacctt ttttctttga caaagagctt tcctccagga
    16681 gaaaagcccc cgtactgcgg tgcatagagg ctctgtaccc ttatgctgcc ctggttgaaa
    16741 acgccctccc gggtcacgta aaaaccttct tcaattacat gagtcccggc gggcaggttt
    16801 tggaaaaagt gttgtgtcga ggcttccttc acttcccgga accatgaaca ggaaatattc
    16861 ccttgatacc ctgaagtttc agactccggc atcaggcaag atgccctgga agctttcaag
    16921 tgtacaaaac tgcggtcttt atcgttgttc aggtggtatc gcgccaggat acgctctccc
    16981 gggcggagtg tgtctccctc accgacggga gcattgtctg cagcacggtg ccatgtccgg
    17041 ttgatttcca ggccgctgga ataaggagcg gccgccgaga agtcttccgt ggtgtgctcg
    17101 tatagactta catacaacag cgcatctgtg cggcttctga ccgtataggt ggttccacgc
    17161 tgcaggatct cgtaccgggc agggctgctt gacacactgc ccccataata cagaagggca
    17221 tggatcacgt ctgtggttcc cgcacgggag gtccagatgt tgttttcctt cctgtcaagg
    17281 atccagcggg tgataccctc acgcaggatg ggatccgtgt tgaagatgcg caacagcagg
    17341 gcatgagctt tcatctgtga actcatgggg ccttcgtagg gtacggcgtt gggaaaatga
    17401 caacccatct ggctgtcgca gacagcaaac tccctcaacg aagcaagcag ggattccagc
    17461 gcctttgttt ctccccaccg ctccagggtg acagccaggt atgctttttc catgaccgat
    17521 gctcctttcc atgattgcag gggattccgg gccaaacggg ctgcgattct tatgatgtcg
    17581 ggtccaaggg ggaacttctg tctgaaagcg gaccggacaa aaaagtacaa gttcacatca
    17641 gtacccagat tgcggattac ctccttatcc gtgttattga tgaacaggct gtctgtatag
    17701 cggagtgctt tccttacgat ggaatataaa ggttcatcat catacgccac gacctccttt
    17761 tctatcatat ccccgatttt ttccaggaaa tgcaacgtca gcaggtaaga actttccata
    17821 cccgggaacc aggaaaaacc accatcctgc cgttgcaatg ccctgaaatg ttccagtgcc
    17881 cgggcattga atgaccggac ataggcggga tctgccaggc gcagcaaccg ctgcatcctt
    17941 tcagcttctt gtcccggata accggaccag ggcgtttctt ccaggagcag cgctcctgtc
    18001 cggttgcttt tttcaaagac ggcagcttgt cctgaaggat gggccctaag ggtatccagg
    18061 atgtggggaa aacgttccag gatgcgggct cccgtgttgt tggcataaaa agcggctacc
    18121 cagcttgtaa gattattgct ttccggttcg caaagaaccg ggagtgcctg taaagcggag
    18181 gaaagcgggg tggttacttc caggttcgcc tctgaggact tgtccttttt caaaagcgtc
    18241 accacaccat ttccttgtaa tgtttttgtc tgagcccggg taacctgttc catcatggaa
    18301 agaacaggga tggtgtgagt ttccgcatcg ctgagggaag ggctttcaaa accggctgtc
    18361 agggtcagca ggggcacgtg caacgtccgg acccggggag cctggaaatc ctgccggaac
    18421 aacaggcggg ccgaggggag cagcgtagcc ggctggcatg ccttatcctt gtcgcctgcc
    18481 ttcagataag ctttgccctg caatgtgtcc ggtgtcaggt tgaccacccg ccaggtcagg
    18541 gaaatactgt ccccggacct tacataggtc gggaaatacg gcaaagccat cacttctttt
    18601 ttcaccgtca gtatttcatc cgccggaatg cttttcccat ctgatgtatg cgccagcacc
    18661 agtatgcgga aagtgctgag cagtccgttt gtccggaatg cgaccggaac atacccggta
    18721 ctgtccggaa ccaggtgcgg caggaaagcc agggtctctt caaaatcact ccggaccaca
    18781 ggtgtctctt cctgttgcgc aggagaaaga gcctcggcca tggcattttc atgactgtca
    18841 tctgttcggg cttgtgccat ttcttcccct gcggccttat tgtttccctg aaccatatag
    18901 ggacttgtcg ccctatacat gacaggtcct ttggtgcccc ctgcgtatcc ggtggtggtt
    18961 tgcatgaaat gatgccggat atatgggatg gaaacatatg gcgcgcgtgc cggctggtaa
    19021 gagaagctgt tgggctggaa gcggtctgta ctcttgttga agatgctcac cagcacctcg
    19081 gctttttcca tggcaggcaa tttaaggccg aaagtttcgc gtgaatacgg tccggccacc
    19141 ttgcgcagcg atacaaattg tatggccggt tccagcccgg gcggttcttc ccagcggtgt
    19201 ctgaattcat ggaaaatccc gtcccggatg gcaataagcc ttaattccag gggataagat
    19261 tcatgaacag cctctccaaa agaaaaggta cgcattccgg gctgcagtac tatgtggtat
    19321 ttttttagga gggaatcggc aacataccat tctgccagtg caaagagctg ccggtcaacc
    19381 gttcccagca ggaaggaagg atcggttgta tccagtgggc agaaaaaagc ggcggtgtct
    19441 gccggactta ccgtgtcctg catacttacc agccagaaag tctctttatg cggaggggct
    19501 ccggccaggt ccagggaaag ctcccattgg ccggattcca tggcagacca ggacggcagg
    19561 gaagtctctt ttccggtaaa ggtgcccgag tactggatca cacggtttcg ggacagggta
    19621 taagtgccgg aaacttcctg gtcatgaccg gtactgttct tcacctccag cgcaaggaag
    19681 ggcccctttt ccttaaccag gatgcggtcc cttgcctcag ttcctgcgga accaaggcgg
    19741 gtatggacgg tatagcgatg attgctcaca ggaaggaagg tttgctgttg ccaggtttct
    19801 cctgaaggat ccgttgcaga aaaatgcacc tgcagccatt cgccccgggg attatcctca
    19861 tcattttttc ccgcaggaat ggctgcgcga aaccagccat cggatccggt aagcaatgta
    19921 tcggtgctac agggttctcc tgctgccggg tataagtgag gatccctgta aaggggacga
    19981 aacatgggtt gtctgtatac acggcaaatc acggcaaccc cgtccagggg gaatcccgca
    20041 taattgccca ctacaccatc ctggataatg gtgtctccat aggcataagc ctgtttgaca
    20101 ggcagcaggt caatggaaaa ggaaggcctg gtatattctt caacccggat cagagccgat
    20161 gcgccaggca tggtcaggct gtgttctccg ttgagcctga ctatcccggg gggaatgtac
    20221 ccgctgaagg atccgtactg gtttgtcaca acttcgacgg tatacagcgt gtccctgtaa
    20281 ctggcattgt tgcgcaagac aaccgtatac ctttttcctg tttccggttc acaccgtctg
    20341 ttgctgtaac gggtaacgat tcccttgaaa aagagggtgt cctgcatcct gtataatttc
    20401 ctgtctgtaa agatcgcggc agatgtggat ttgtcgaaag ggtagccgta actgaaatcc
    20461 cagggatgca cggtaagggg tactgcaaaa gtatccggta ccgctctcag gggtgctgga
    20521 ggagacaggc ggaacgattc gcccgtacgc acatccacgg gtgtgaatcc gtccatgtct
    20581 atggagaaag atggcctggc gtcctttaca aaccggactt ctgcctttgt aaagggttca
    20641 ccggtgttaa gatcggccgc gtatacgtag cccgtgttgc ggaatgaacg gaacatggaa
    20701 gcaaccgtac cgctgtaaaa agcagtttcg cttgctgtac ggcctttttt gacacgaatc
    20761 ctgtaggatc cggcaggcgg tgcctgcaac agggtaagtg tatcggttac ggcttcatat
    20821 accggctcat ttaatcagct c
//
LOCUS       DAOSOF010000015        20515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2597_length_20515_cov_4.773069, whole genome shotgun sequence.
ACCESSION   DAOSOF010000015 DAOSOF010000000
VERSION     DAOSOF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_20515_cov_4.773069"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLM68_01690"
     CDS             <1..950
                     /locus_tag="PLM68_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT54117.1"
                     /translation="EIKAGDILVKIPRAIGKLRDITGGLPRVTELFEARNPSNPAIVS
                     EIDGEVLMGRIKRGNREIIIRSKTGEEKKYLVPLSKQILVQENDYVRAGMALSEGAIS
                     PTDILLIQGPTRVQEYIVNEIQSVYRNEGVKINDKHFEVIVRQMMRKVEIVDPGDTRF
                     LPEQLVDKWEFMQENDSIFDKKIITDAGDSQELKPGQMVTVRQLRDENSTLKRKDLKP
                     VQSRDAIPATSNQILQGITRAALRTNSFMSAASFQETTKVLSEAAIFGKSDTLEGLKE
                     NVICGHLIPAGTGQREFENILVTSFSDYKKMVEERTLPK"
     gene            1049..2893
                     /locus_tag="PLM68_01695"
     CDS             1049..2893
                     /locus_tag="PLM68_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT54118.1"
                     /translation="MPHKVIDVQDVVIRFSGDSGDGMQLTGSLFADTAAVFGNDITTF
                     PDFPSEIRAPQGTLSGVSGFQVHIGSSAIYTPGDYCDVLVAMNPAALKANVKWLKPNA
                     TIIVDIDTFEKKGLEKAGFTTDDAFDELNIQRYNIIAPPITTLTKEACIACSIDPKTG
                     IRCKNMFALGICYFLYNMPLKHTETYINKKFARNPDLININLRILKDGYNYAGNIQAI
                     ADTYHVKPSRHEPGLYRNINGNRAIAWGLMAASEKSGRPLFCGSYPITPATAILEELA
                     IHKYLGVKTLQCEDEIAGICTAIGASYAGSLAVTSTSGPGLSLKSEALGLAVMAELPL
                     VVINVQRAGPSTGIPTKTEQTDLNQALYGRNGECPVAIMAPHSPSHCFVTAFFAAKYA
                     LEHQMPVIVLSEGFLGNGSEPWKIPDMNDYPAINPPIVTELDPDEPYFYPFKRNPETL
                     ATRWALPGTPNLEHRVGGLEKNHRGAVSSDPLVHEQMVTERAEKVARLARYIPRQQII
                     GEQEGDILMVSWGGTYGHMLSAESQLQAEGIKVGLAHFDYIRPLPINTEDVLKRFKKI
                     IVFELNSGHFANYLRAQYPQFSYLQYNKIQGQPFIVAEITEAIKKIVK"
     gene            2890..3909
                     /locus_tag="PLM68_01700"
     CDS             2890..3909
                     /locus_tag="PLM68_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOT54119.1"
                     /translation="MNYTPQDFKSDQEVKWCPGCGDHAVLASIQRALPEISEALGYSK
                     ERYVFVSGIGCSSRFPYYMKTYGFHGIHGRAAAIATGIKVANPTLSVWEMTGDGDSLA
                     IGGNHLIHAIRRNIDINIVLFNNRIYGLTKGQYSPTSKWGTQTKTSPYGTVEHPFNPG
                     ALAMGARGTFFARTLDMELQLTRDIMVEAAKHDGTALVEVLQNCVIFNDKTHSVISDK
                     EVRAERTILLRHGEPMLFGKDRNKGLVLDGIRLRAVTLGQDGVTMDDILVHNAEEQNH
                     GLHMMLCEMEWPELPVALGVIRRVKDRTYDDMVRDQLTEVANKSEIHCMNDMLRSGGD
                     TWVVE"
     gene            3948..5285
                     /gene="gdhA"
                     /locus_tag="PLM68_01705"
     CDS             3948..5285
                     /gene="gdhA"
                     /locus_tag="PLM68_01705"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT54120.1"
                     /translation="MKAKEIMSALEAKYGHEKEYLQAVHEVLESIEDVYNQHPEFEKA
                     RIIERLVEPDRIFTFRVTWVDDKGEVQVNTGYRVQYNNAIGPYKGGIRFHRSVNLSIL
                     KFLGFEQIFKNALTSLPMGGGKGGSDFDPRGKSANEMMRFCQAFMTELWRHIGPDTDV
                     PAGDIGVGAREVGYMYGWYKKLAHEHTGVLTGKGLTWGGSLIRPEATGYGGVYFVEHM
                     LQALGQNIKNKTVAISGFGNVAWGAAQKAHELGAKVITISGPDGYVYDKDGMNEEKIA
                     YMLDLRASGNDIVAPYAEKFPGAVFHAGKKPWEVKVDIAMPCATQNELNEDDAKKLIA
                     NKVMMVAEVSNMGCTPQAIEAFQHAKVSFAPGKAVNAGGVATSGLEMTQNAAHLSWTA
                     EEVDAKLHQIMSSIHQACVEHGKQKDGYINYVKGANIAGFMKVARAMLEQGVI"
     gene            5288..6148
                     /locus_tag="PLM68_01710"
     CDS             5288..6148
                     /locus_tag="PLM68_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54121.1"
                     /translation="MFRLGLDFGSTLTKMALIKPGVKDKPFPQAVFKYKTNDHPGDFH
                     NALVDFLKRENIPPDAIEHISATGTHANMAKDNILGIPVNKVPEVESIGLGGLALSGL
                     DEALVVNMGTGTTYVDATAEGIHHLGGTGMGGGTLTGLGQCILGTSSVREIVRLAGHG
                     KLENVDLLISDITDQELQNLPPILTAANFGKLAHQLYAKQASLAGKEDLARALINLVL
                     QVIGSMAVMVCKGSNRRNVVFVGTLTSLPQIGETYSIFSKVYGLQFIVPRFSVYGTAL
                     GSTLVSAEEV"
     gene            complement(6043..6858)
                     /gene="murI"
                     /locus_tag="PLM68_01715"
     CDS             complement(6043..6858)
                     /gene="murI"
                     /locus_tag="PLM68_01715"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT54122.1"
                     /translation="MNSSPIGIMDSGIGGLGVLNLIKALLPGERLIYVADKAYCPYGS
                     LPVEEIRHRCILISLFFLSHNCKMIVIACNTATAAAIETLRSTFTGIPVVGMEPAVKP
                     AVLHTHTGIVGVLATPGTFKGRLYNRTLATFGGQVKVIERVGEGWVGAVERGDLDTPA
                     TREMVRKTIQPMLDEGVDHLVLGCTHYPFLIPVIRSIAGPGVVIVDPAPAVARRVKNL
                     LTNNGLLSGGVPDGEKDLFYTSSADTSVLPKAVPYTENLGTMNWRPYTFENME"
     gene            complement(6872..7429)
                     /locus_tag="PLM68_01720"
     CDS             complement(6872..7429)
                     /locus_tag="PLM68_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT54123.1"
                     /translation="MKRISTVLFLLTCIIGTSSAQNLKFGHINSQELIGLMPERDSAL
                     IKLQNYTNEVEQTMADMQQEYQSKVNAYQQKQATWTAAVLEAKQRELLEIQQRLEQFN
                     QTAQQEYQQMQQIFLSPVFQKANAAIAKVAKDNGFTYIFDLSAGGVIFFDEKVSVNIL
                     PMTKNELGIPAEKVAPTQLPGMEAQ"
     gene            complement(7469..7981)
                     /locus_tag="PLM68_01725"
     CDS             complement(7469..7981)
                     /locus_tag="PLM68_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT54124.1"
                     /translation="MKRMVLFLMICMVAGSLYAQKYAIIHSQNILSALPEYTSAQEQI
                     ARLQEQYQEKLQAEYDALEEMFQQYQKDKADLTAQQRRIYEQQILDREKEVKALQEQY
                     FGQEGTLAKTSEVLFKPIQERMQDTVRQIAESQGYSMVLDIASGMGVIYYAPQTDISD
                     LVIARLQGVR"
     gene            complement(7993..10533)
                     /locus_tag="PLM68_01730"
     CDS             complement(7993..10533)
                     /locus_tag="PLM68_01730"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOT54125.1"
                     /translation="MKHLFFCITFSLLVLPIFAQVTEQAATTDTTGRKQSSVVIDYTS
                     PTKCVIAGLQVTGVNYLNADQILSLTGLALGDTISIPGDELKTIVKRLFMQRFFSDVA
                     VYVDSLVNNNAYLNISITERPRVSQWKYDGVKKGEQTDIEERVRLRRGSELSEYVVKS
                     SSDIIRKYFVEKGFLNARVTATHQEDTTIKNAVQVTFHVERNQKVRIKQINFLGSDQL
                     SLLKMRFAMKKTKDKRLYNFFFSKKFDEKEFQNDKTLLLQKFHELGYRDARITKDSIY
                     FIDPGNLGIDIHIDEGKKYYFRNITWTGNTQYTGEMLNSVLRIGKGDVYDVVTMEERL
                     FGGGKLQQSNVSQIYKDQGYLFFRVVPIEVNIEQDSVDVEMRIFEGKPATYNHIIING
                     NNVSNEKIIRRQVFTRPGYLFSQTELERSIREIASMGHFDQEAIMQMGSGFNVVPNQV
                     NNTVDIIYNVTEKPDSQFEIAGGWGGNTFVGTVGISFNNFSIKRLFDKQAWRPVPLGD
                     GQTLSLRFQTNGTYYMALSVNFVEPWLTGKKPTSFSTSIYYTRQTASTYYYLTNDQFM
                     EVYGLGIGLGTRLKWPDNYFVFYNQLDIKRYNLQDWGYYFMFTDGQSNIIDYSITLQR
                     NSTDQIIYPRQGSDLKFGLQVTPPYSLFRDKNTDYASMTDPQRYRWIEYHKWTFDANL
                     YTRLIGDLVLMTRANFGYLGYYSHKLGYSPFEGFMLGGDGMSGYNTYGADIISQRGYN
                     NYSLTPTINDAYAGRVYTRFTVELRYPVILQPQSTIYALLFLEGGNCWSDIRDFNPFS
                     IKRAAGVGVRVYLPMVGLLGIDWGYGFDTDFSGKKGGSNFHFMIGQQF"
     gene            complement(10544..11260)
                     /gene="uppS"
                     /locus_tag="PLM68_01735"
     CDS             complement(10544..11260)
                     /gene="uppS"
                     /locus_tag="PLM68_01735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOT54126.1"
                     /translation="MVKQIEKLPDHVCIIMDGNGRWALSRGLERNVGHRQGVEAVRAA
                     TEYAAEKGIPYLSLFAFSEENWGRPKSETDAIMSVIVNAIHNETPKMMKNNIRCVFIG
                     DREQLDNKALKRMDDCMRQTAGNTGMTLIVAVNYSGKWDILQAVKRLLEENPPAEQLL
                     SPEVFSSYLSTAGVPEPDLMIRTSGEMRISNFMLWQMAYTELYFTPVLWPDFRKPDFE
                     AALNAYSNRKRRFGKIEESI"
     gene            complement(11253..12062)
                     /locus_tag="PLM68_01740"
     CDS             complement(11253..12062)
                     /locus_tag="PLM68_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT54127.1"
                     /translation="MKVVFYGKDLKKDFHLPERKDISFQMLGQDKILPAGTDLLVTVG
                     GDGTFLEALEIVKDSGVPVAGVNFGRMGFLTGCGPDFIGYLFDAVLNKQFSTTSHTVL
                     TIEREDLQKRIYPYAINEICIQRLSSAMIEVDVKINGSQISTYRGDGIIVATPTGSTA
                     YSLSVGGPIVFPGSGVLILSPIAPHNLNVRPIVFPDNAILAIQVRSRTPNAQLHLDNR
                     SEIVDLPYSFIVKKARFSITSLTFEGNDFVTTLREKLMMGFDKRDGGQNYG"
     gene            12113..12832
                     /locus_tag="PLM68_01745"
     CDS             12113..12832
                     /locus_tag="PLM68_01745"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOT54128.1"
                     /translation="MTKLSVNINKFATLRNARGGNMPDVLKAAIDCERFGAQGITVHP
                     RPDERHIRYQDARDIKPIITTEFNIEGNPIPSFIQLVLEVRPDQVTLVPDAHDAITSN
                     AGWDTIRHASELREWVGLFRNAGIRTSLFVDPNPDMVRAAALTGTDRVELYTEAYARM
                     YDADREKAIAPYIAAAEAAVDCSLGLNAGHDLNLTNISYFNKNIPGLLEVSIGHALIC
                     ESIYLGLENTIQLYLRHLSNF"
     gene            12862..13470
                     /locus_tag="PLM68_01750"
     CDS             12862..13470
                     /locus_tag="PLM68_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOT54129.1"
                     /translation="MKKSSLIITILLALAIAALYVLHFTGIGTGKPVADKDDMQGSET
                     LSGSIVYIRIDSLLNAYDFFLELQADLAAKARIVKEDLNKKGRAFENDVTNFQDKVQK
                     GLITRAQAETQQAQLAARQQELEQYAQQKQVELAEENQVMLNRVLDALKTFLAEYRLL
                     KNYDLILTTDGTSNTVIEAVSSMDITKDVVEGLNREYSKSGK"
     gene            13480..14430
                     /locus_tag="PLM68_01755"
     CDS             13480..14430
                     /locus_tag="PLM68_01755"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT54130.1"
                     /translation="MKSVTATQFHFAKQTGLYIGKVRDVYTIDGRILVMIATDRISAF
                     DIVFPKGITYKGQILNTIASRFLDSTHDIVPNWKIASPHPMVTVGYRCEPFPVEMIVR
                     GYLTGSSWRTYKSGQRSLCGIPLPDGMREHQAFPAPVITPTTKAGIGSHDQDISGEEI
                     IGRGLVSEEDYRKIEAFSLALFERGQKLAAEKGLILVDTKYEFGKLGTQIYLIDEIHT
                     PDSSRYFYAEDYERNYPLGLPPRQLSKEFVREWLMEKGFSGQPGQKMPELTDTVIQTI
                     SERYIELFEHLTGTAFVPLVYDDTFYDNMEGCIRNMLARL"
     gene            14427..15794
                     /locus_tag="PLM68_01760"
     CDS             14427..15794
                     /locus_tag="PLM68_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT54131.1"
                     /translation="MKIRNIFVIFLLFASLSLYAQEQHTIRHKVRWMETLYSIARKYK
                     VDPKDIALLNDLKTGEISRGQILLIPVPDAGLAEQEEEPLQDTLLVEEEHEMPVKWKT
                     PCRDYVHPPAWEPVVSVILPFPGTMAGAGFVEFYQGVLLAADRMKNQGMPILLQVFDW
                     NEKPMDELLSGSSLGQSNLIIGPVYSNQVGSTLAYFRNSETKIVSPLDSNAEVWTSLY
                     PNFFQVQPPLAAQQESLLRYLEPHDATLWVISEEGEETVAPGIRALLDKNLIQYRNFS
                     YDVLMGREVTEVLRELLGANPRNQIIIASLNEAFVSDAIRNLHLLLAYDNIPVELFGF
                     SRWRSFETLDLAALHQLQVVLPLTAFVDYSDVGVKAFVQDYRSRFHGEPSQFSFQGYD
                     VALFFLNALFQYGPDFEDCISNISVPLLQNRFTFVKTLAGGGFSNTGTRVVRYRPDFT
                     IELLP"
     gene            complement(15795..16586)
                     /gene="nagB"
                     /locus_tag="PLM68_01765"
     CDS             complement(15795..16586)
                     /gene="nagB"
                     /locus_tag="PLM68_01765"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT54132.1"
                     /translation="MRVLILENYDRLSVWAARYIAEKINTYKPTHDNPFVLGLPTGSS
                     PLGTYKELIAMHKAGKVSFKNVVTFNMDEYMGIPKDHPQSYHYFMQENFFRHIDINPV
                     NTHVPDGNATDPVAECRNYEEAIKRTGGIRLFMGGVGVDGHIAFNEPGSSLASRTRIK
                     TLTSETRMANARFFDNDMERVPKTALTVGIGTIMDAEEVLILVSGENKARALRHAVEG
                     AVSHMWTITALQMHPGAIIVCDQAATAELKAGTVRYFKDIEKDNL"
     gene            complement(16583..18250)
                     /gene="recN"
                     /locus_tag="PLM68_01770"
     CDS             complement(16583..18250)
                     /gene="recN"
                     /locus_tag="PLM68_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT54133.1"
                     /translation="MLYSLSITNYALINILDIEFSEGLTIITGETGAGKSIILGALSL
                     VLGQKADAGILRDPGSNAVVEACFRIPGNDNALEQLFSADEVNYSGELIIRRVIYPNG
                     KSRSFVNDQPVNVAFLRSLGNALIDIHSQHESLLLADTDYPVRVADAFAGQTQLADEY
                     RKKHAEVTSLAARVRTLRDACDQAKNETDYLSFQYQELQKAKLSPGEQEELEKELSLL
                     EHAAEIRQYLFQLSSLLEEGDFPVLTGLKEAVRVSAQITEKNPGFSDVSDRMEQARIE
                     LKDLAGECARSLQQVRVDPVRMEEVSGRLDTIYTLQGKHNVRTVEDLLILEKDFGRQL
                     EETVNDTTRLEKLEKELEAAIAIRDQLATDLHKGRMACLDDLSRQLCTRLALLGIPDA
                     QIEFVIREKATYGLLGNTGMEIMFSANRDMMPRELSCIASGGEMSRLMLCIKSVIAQH
                     SGLSTIIFDEVDLGVSGKIADRMGNMIYQLSEYMQVLAITHLPQVAAKGSTHYLVRKE
                     VVEHVTETRVLPLHGDERVMEVARMLSGSAITPAAIQNAKELLNNIS"
     gene            complement(18253..18579)
                     /locus_tag="PLM68_01775"
     CDS             complement(18253..18579)
                     /locus_tag="PLM68_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT54134.1"
                     /translation="MESKKQPIPDTTVTRDLSKFSEKTGNIYQTVMIIAQRANQIASE
                     VKSELNSRLEEFSNFTDTLEETFENREQIEISRYYERQPKPTLLAINEFEEGETYYKV
                     VEEPKE"
     gene            complement(18592..19389)
                     /gene="bamD"
                     /locus_tag="PLM68_01780"
     CDS             complement(18592..19389)
                     /gene="bamD"
                     /locus_tag="PLM68_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOT54135.1"
                     /translation="MKKHFLTFVLLLLTVAPACVNQYEVLLRSTDYDLKFRKAIEYFE
                     AKKYSRSQPLLDQLQMVYRGTVQDDTLQYYLGLSNYHLNNFLVAEDNFKQFVEIFPRS
                     PFTKSARFMRLDCLYQNTYRYELDQQPSRVAIAAIEEFIYDYPGSEYQEVCRKMLGDL
                     YQRLDRKAFESARLYYTIEDYKAATHAFKETLKENPESMYREEILYYIVAANYQYSNN
                     SLPELQRGRFLNVIDEYYNFVSEFPDSKYRREVDNMFRKAQQFTTKQ"
     gene            complement(19428..20360)
                     /gene="trxB"
                     /locus_tag="PLM68_01785"
     CDS             complement(19428..20360)
                     /gene="trxB"
                     /locus_tag="PLM68_01785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT54136.1"
                     /translation="METIKCLIAGSGPAGYTAAIYAARANLQPVLYEGVLRGGQLTTT
                     TEIENFPGYPEGVQGSQLMEDIRKQALRFGTDFRTGIVTETNLSEPPYRIRFDNGTEV
                     LTQALIIATGATARYLGLESEQKFKGMGVSACATCDGFFYRGKDVAVVGGGDTACEEA
                     IYLSALCRKVYMIIRRNVLRASQVMQKRVLSTPNIEILWEHQTAEILGDDSGVTGARL
                     VHKSGRERIINVPGFFLAIGHHPNSEPFLPWVEVNPEGYIITQPKSTETNVKGVFAAG
                     DVQDSKYRQAITAAASGCMAAIDLERYMSMQYGL"
     gene            20426..20499
                     /locus_tag="PLM68_01790"
     tRNA            20426..20499
                     /locus_tag="PLM68_01790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20460..20462,aa:Pro,seq:ggg)
BASE COUNT         5375 a         4962 c         4804 g         5374 t
ORIGIN      
        1 aggagatcaa agccggggat atattggtca agattccgcg tgccattgga aaattaaggg
       61 atatcaccgg gggtctgccc cgtgtgaccg agctgttcga ggcacgtaac ccatccaacc
      121 cggccattgt ttccgagatt gacggagaag tcctgatggg aaggatcaag agagggaacc
      181 gcgagataat catccgctca aaaacaggag aggaaaagaa atacctggta cccttgtcca
      241 aacagatcct tgtccaggag aacgactacg tccgtgcagg aatggccctt tccgaaggcg
      301 ctatctcccc caccgatatc ctgttgatcc agggacccac ccgtgtacag gaatacattg
      361 tcaacgaaat ccagagtgta taccgtaacg agggcgtcaa gatcaacgat aagcactttg
      421 aggtcatcgt ccgccagatg atgcgcaaag tagaaattgt tgatccggga gacacccgtt
      481 tcctgcccga gcaattggta gacaaatggg aattcatgca ggaaaacgat agcatctttg
      541 ataagaagat cattaccgat gcgggagatt cccaggaact caaaccaggc cagatggtta
      601 ccgtccgtca attgagagat gaaaattcca ccctcaaacg gaaagacctc aagccggttc
      661 agtccaggga tgccataccg gccacatcaa accagatatt acagggtatt acacgtgcag
      721 ccttgaggac aaatagtttc atgtcggctg cctcattcca ggaaacgacc aaggtgttat
      781 cggaagctgc catatttggc aaaagcgaca cactggaagg cttgaaggaa aatgtgatct
      841 gcggtcacct gatccctgcc ggtactggtc agcgggaatt tgaaaatatt ttggttactt
      901 cgttctcgga ctataaaaaa atggttgaag aacgaacctt acccaaataa tcatgttttt
      961 tattacaaat aatcaataat aagggcgcat gcccttatta ttttttatac atttgcctga
     1021 attaataaca aatcaatatt tcagctctat gccgcataaa gtaatcgatg ttcaggatgt
     1081 tgtaattcgc ttttccggag actccggtga cgggatgcaa ttgacaggat ccctgtttgc
     1141 cgacactgct gccgtttttg gtaatgatat cacaactttc ccggattttc cttccgagat
     1201 tagggccccc cagggtaccc tgtccggagt gtcgggattc caggtacaca ttgggagcag
     1261 cgccatatat actcccgggg attattgtga cgtgctggtt gccatgaatc ctgctgccct
     1321 caaggcaaac gtaaaatggc tgaaacccaa cgcaacgata atcgtagaca tagacacctt
     1381 cgaaaagaag ggccttgaaa aagccggctt cactaccgat gatgcctttg acgagctcaa
     1441 tatccagcgc tataatataa tagcacctcc catcacaacc ctgacaaaag aagcttgcat
     1501 tgcctgcagc atcgatccca agacgggaat ccgttgcaag aatatgtttg cactgggaat
     1561 ttgctacttc ctttacaata tgcctctcaa gcatactgaa acatatatca ataagaaatt
     1621 tgccagaaat ccggatctga taaatatcaa tttaagaata cttaaggatg ggtataatta
     1681 tgccggtaac atccaggcca tagccgacac ataccatgtt aagccttcca ggcatgaacc
     1741 gggcctttac cgcaacatca acgggaaccg ggccatagcc tggggtttga tggctgcttc
     1801 ggagaaatcg ggcagacctc tattttgcgg ctcctaccct attacacctg ccacggcgat
     1861 cctggaagaa cttgccattc ataagtatct tggcgtaaaa acactgcaat gtgaagatga
     1921 gatagccggt atctgtaccg ctatcggagc ctcatatgca ggatctctgg ctgtaacttc
     1981 cacttccggg cccggccttt ctttgaaatc cgaggcactt ggccttgccg ttatggcaga
     2041 attgccgctg gtagtgatta atgttcaacg ggccggacct tcaacaggta tccccacaaa
     2101 aaccgaacag acagacctga accaggcact ttacggtcgt aacggggaat gccccgtggc
     2161 aataatggca ccgcactcgc cgtcacattg ttttgtcact gctttttttg cagccaaata
     2221 tgccctggaa caccagatgc ctgtaatcgt cctttctgaa ggatttcttg ggaacggctc
     2281 agaaccgtgg aaaattccgg acatgaacga ttacccggca atcaaccctc ccattgttac
     2341 cgagctggat cccgatgaac catatttcta tcctttcaaa cgcaatcccg aaacgctggc
     2401 aacacgctgg gcattgcccg ggacacccaa ccttgaacac cgggtgggtg gcctggaaaa
     2461 aaaccatagg ggtgctgtct cgtcagatcc cctggttcac gaacaaatgg taactgaaag
     2521 ggcagaaaag gtagcccgtc ttgcccgcta cattccccgg cagcagatta tcggagaaca
     2581 ggagggagat atactgatgg tgagctgggg aggcacctat ggacatatgt tgagtgccga
     2641 aagccagtta caggcggaag gtataaaagt gggtctggca catttcgatt acatcagacc
     2701 gctacccatc aatacggaag atgtgttgaa acgctttaag aagatcattg tttttgaact
     2761 caatagcgga cattttgcca attacctgag agctcagtac cctcagttct catacctgca
     2821 atacaacaag attcagggtc agccatttat tgtagcagaa ataacagaag ccatcaaaaa
     2881 gatagtaaaa tgaattacac tcctcaggat ttcaaatcag accaagaagt gaaatggtgt
     2941 cccgggtgcg gggaccatgc cgttttggcg tccatacagc gtgccttgcc ggagatcagt
     3001 gaagctttgg ggtactctaa ggaaaggtat gtgtttgtgt caggaatagg ttgctcctct
     3061 cgttttccat attacatgaa aacatatgga tttcatggga tccacggtcg tgccgctgcc
     3121 attgccacag ggataaaagt agccaaccct acactttctg tatgggaaat gaccggagac
     3181 ggagattccc tggctattgg gggaaaccac ctgatccatg caatcagaag gaatatcgac
     3241 atcaatatag tgttgttcaa caaccgtatt tacggcctga ccaagggcca gtattcgcca
     3301 acatccaaat ggggaaccca aaccaagaca tccccctatg gaacggttga acatcccttt
     3361 aatcccggag cccttgccat gggtgcgcgt ggaacctttt ttgcccgtac tcttgacatg
     3421 gagcttcaat tgacccgtga tatcatggtg gaagcagcaa aacacgatgg taccgcttta
     3481 gtggaggtac tgcagaactg tgtcatattc aatgataaaa cccactccgt tatctctgac
     3541 aaggaggtaa gggcagaacg caccattttg ctccgccacg gagagcccat gttatttgga
     3601 aaagacagga ataagggcct ggtcctggac gggatcaggt tgagagctgt taccctcggg
     3661 caggatgggg tgaccatgga tgatattctg gtccacaatg cagaggaaca aaaccatggc
     3721 ctccacatga tgctgtgcga gatggagtgg ccggaacttc ctgttgccct tggggtgatc
     3781 cgccgcgtta aggatcgtac atacgacgat atggttcggg atcagcttac ggaagtagcc
     3841 aacaaatctg aaatccactg tatgaacgac atgctgagaa gcggtggaga tacctgggtt
     3901 gtagaataat tgttaactat caataatcac ttaattcatt tataattatg aaagcaaaag
     3961 aaataatgtc tgctcttgag gccaaatacg ggcacgagaa agagtacctg caagcagttc
     4021 acgaagtttt ggaatccatt gaagatgttt acaaccaaca tcctgaattt gaaaaagcca
     4081 gaatcattga gcgtcttgta gagcctgaca ggattttcac attcagggtc acctgggtag
     4141 acgacaaagg agaggtacag gtcaacacgg gctaccgtgt ccagtacaac aatgccatcg
     4201 ggccgtacaa aggaggtatt cgctttcacc gttcggtgaa cctttccatc cttaaattcc
     4261 ttggatttga gcagatattc aaaaacgcac tgacctcctt acccatgggt ggtggtaaag
     4321 gcggttctga ttttgacccg cgcggcaaat cggcaaacga aatgatgcgc ttttgccagg
     4381 cttttatgac cgagctttgg agacatattg gtcctgacac tgacgttcct gccggtgata
     4441 tcggagtggg agcacgcgag gttggttata tgtacggatg gtataaaaaa ctggcacatg
     4501 aacatacggg tgttctgaca gggaaaggac ttacatgggg aggttccctt atccgtccgg
     4561 aagccacagg ttatggcggt gtttattttg ttgagcatat gcttcaggct ttaggccaga
     4621 acatcaaaaa caaaaccgtt gccatatccg gtttcggaaa cgttgcctgg ggtgctgccc
     4681 aaaaggccca cgaactggga gccaaggtga tcaccatatc aggacctgat ggttacgttt
     4741 acgacaaaga cggcatgaat gaagaaaaga tcgcctatat gctggatttg cgtgccagcg
     4801 gtaacgatat tgtagctccc tatgcagaaa aattccccgg tgctgtattc catgcaggca
     4861 agaaaccctg ggaagttaaa gtggacattg ccatgccttg tgctacccag aacgaactca
     4921 acgaagatga cgccaaaaaa ctgatagcca acaaggtgat gatggttgcc gaagtttcca
     4981 atatgggttg tacaccccag gccattgaag ctttccaaca tgcaaaagtt agctttgccc
     5041 ccggtaaggc agtgaacgcc ggcggagtgg ctacctcggg actggaaatg acacagaacg
     5101 cagctcacct gagctggact gcagaagagg ttgatgccaa actgcaccag atcatgtcaa
     5161 gcatacatca ggcttgtgtt gagcatggca agcagaaaga tggctatatc aattatgtaa
     5221 aaggcgccaa tattgcaggg ttcatgaaag ttgcccgtgc aatgcttgaa cagggagtga
     5281 tatagtcatg ttcagactgg gccttgattt tggttctaca ctgactaaaa tggctctgat
     5341 aaagcctggg gttaaggata agccttttcc ccaggctgtt tttaaatata aaacaaacga
     5401 tcatcccggc gatttccata atgcactggt agatttcctg aagcgcgaga atatacctcc
     5461 ggacgccatt gagcacatat cggctacagg aacccacgcc aatatggcaa aggacaatat
     5521 cctgggaatc cctgttaaca aagttccgga agtggaatcc atcggccttg gcggcctggc
     5581 tctgagtgga ctggacgagg ctcttgtggt gaacatggga acaggcacca cctatgttga
     5641 tgccactgcc gaaggcatcc atcacctggg gggcaccggt atgggaggcg gaacgctgac
     5701 cgggctgggc cagtgtattc ttgggacctc ctcggttcgg gagattgtcc ggttggccgg
     5761 gcacggaaaa ctggaaaatg ttgacttgct gataagtgat ataacagacc aggaattgca
     5821 aaatctcccc ccaatcctga cagcagccaa tttcgggaaa ctcgcccatc agctctatgc
     5881 aaaacaagcc tccctggcag gtaaggagga tctggcccgt gctttgataa accttgtgtt
     5941 acaggttata ggaagcatgg ccgtgatggt atgcaaagga tctaacagga gaaacgtggt
     6001 atttgtggga accctgacga gcctgcccca aatcggggaa acctattcca tattctcgaa
     6061 ggtatacggc ctccagttca tagttcccag attttcggta tatggtacgg ctttaggcag
     6121 tacgcttgta tctgcggaag aagtataaaa caaatccttt tccccgtcag ggacaccacc
     6181 tgataataat ccgttatttg tgagcaggtt ttttacacgg cgggcaactg caggtgccgg
     6241 atcgacaatc accacgccgg gtccggctat gctgcggata accggtatca ggaacgggta
     6301 atgagtacat ccaaggacca ggtggtcgac accttcatcc agcatcggct ggatggtttt
     6361 tctgaccatt tcacgggtag ccggcgtatc caggtctccc cgttcaaccg ctccgaccca
     6421 gccttccccc accctttcaa taacttttac ctgaccgcca aaagttgcca gggtcctgtt
     6481 gtacagtctt cccttgaaag ttcccggggt agccagcact cccacaattc cggtatgtgt
     6541 atgcaatacg gcgggcttta cggcgggttc catacctacc acgggtatgc cggtgaaagt
     6601 cgaacgcaag gtttcaatag cggcagccgt agctgtgttg caggcaatca ctatcatctt
     6661 gcagttatgt gacaggaaaa aaagcgagat gagtatgcac cggtgcctta tctcttccac
     6721 tggcagagaa ccgtaaggac aataagcctt atctgcaaca taaatgaggc gctcccctgg
     6781 gagaagcgcc ttgataaggt tgagaacgcc cagcccacct atgccggaat ccattattcc
     6841 aatgggcgaa ctgttcatag gatcaatttt cttattgtgc ctccattccg ggtaattggg
     6901 taggagccac tttttcggcc ggaataccta attcgttttt agtcataggc aatatattga
     6961 cgcttacttt ctcatcaaaa aagataacgc ctccggcaga aagatcaaat atataagtaa
     7021 atccattatc ctttgccacc ttggctatgg ctgcattggc tttctggaaa actggcgaaa
     7081 ggaaaatctg ttgcatttgc tgatactcct gctgtgctgt ctggttaaac tgttccaggc
     7141 gttgctggat ttctaaaagt tcacgttgtt tagcttcaag caccgcagct gtccatgtgg
     7201 cttgtttctg ctgataggcg ttgactttgg attggtactc ctgttgcata tcggccatgg
     7261 tttgttcaac ctcgtttgta taattctgca atttgatcag ggcagaatcc ctttcaggca
     7321 tcaaaccaat cagttcctgg gagttgatgt gcccgaattt gaggttttgt gcagaagagg
     7381 ttccaattat acaagtcaac aaaaaaagga cggtggaaat acgtttcata agttcatggt
     7441 ttataaagaa tacaaatgta caaaaaaatt acctgacacc ctgtaatctt gcaataacca
     7501 gatcacttat gtctgtctgt ggagcataat aaataacacc catacctgac gcaatgtcca
     7561 ggaccatact gtatccctgg ctttcagcaa tttgacgcac tgtatcctgc atgcgttcct
     7621 ggatgggttt gaagagcact tccgatgttt tggccagtgt tccttcctgt ccaaaatact
     7681 gttcctgtaa tgcctttact tccttttccc tgtcaaggat ctgttgttcg taaatacgtc
     7741 tctgctgtgc agtaaggtct gctttatctt tctggtactg ctggaacatt tcttccaggg
     7801 cgtcgtactc agcctgcagc ttttcctggt attgttcctg caacctggca atctgttcct
     7861 gggcagaagt atactcgggc aatgcactga gtatgttttg ggaatggata attgcatatt
     7921 tctgggcata aagactgcct gcgaccatgc aaatcattaa aaataaaacc attcttttca
     7981 taatactgat gtttaaaatt gttgtccaat catgaaatgg aaattcgatc cgcctttctt
     8041 tccactgaaa tcggtatcga acccatatcc ccagtcgatc cccaacagac cgaccatggg
     8101 tagataaacc cttacgccga ctcctgctgc tcgctttatg gaaaagggat taaaatcacg
     8161 tatgtcactc caacagttcc ctccttccag gaacagaaga gcatagatag ttgattgtgg
     8221 ctgcaatatc acaggataac gcaattctac ggtgaacctt gtatacacgc gtccagcgta
     8281 agcatcgtta atggtaggtg tgagtgaata gttgttgtaa ccacgctggg atatgatatc
     8341 ggctccatat gtgttgtaac cgctcatacc gtcgcctccg agcataaaac cctcaaaagg
     8401 cgaatagccg agcttgtgac tgtaatagcc cagatagccg aaattggccc tggtcatcag
     8461 gaccaggtct ccgataagcc gcgtgtacag atttgcatcg aaagtccact tatggtactc
     8521 gatccaacgg taccgctgcg gatcggtcat agatgcgtaa tccgtattct tatccctgaa
     8581 aagggaatag ggaggtgtca cctgcaatcc gaatttgagg tctgatccct ggcggggata
     8641 tatgatctgg tctgttgaat tgcgctgcag tgtgatactg taatcaatta tgttggattg
     8701 cccgtcggtg aacatgaaat agtaacccca gtcctgcaga ttgtatcgtt tgatatccag
     8761 ctgattgtaa aatacaaagt aattgtctgg ccattttagc ctcgttccca ggccgatgcc
     8821 cagcccgtaa acttccatga actggtcgtt ggtcaggtaa tagtaagtag aagcggtttg
     8881 ccttgtataa taaatgctag tactgaatga cgtgggtttt tttccggtca gccagggctc
     8941 cacaaaattg acgctcagag ccatatagta agtgccgttt gtctgaaacc tgagggagag
     9001 agtctgtccg tctcccaggg gaacgggacg ccatgcctgc ttgtcaaaaa ggcgcttaat
     9061 agagaaatta ttaaaactga tacccacggt tcccacaaag gtgttgccac cccagccgcc
     9121 ggcaatttca aactggctgt cgggtttttc tgtcacatta tagatgatgt cgaccgtgtt
     9181 gttcacctgg ttgggaacca cattgaaacc gctccccatc tgcatgatgg cttcctggtc
     9241 aaagtgaccc atagaggcaa tttccctgat agacctctcc agttctgtct ggctgaacaa
     9301 gtagccgggc cgggtaaaaa cctgcctgcg gatgattttt tcattggaga cgttattgcc
     9361 attgatgatg atatggttat aagtggccgg cttgccttcg aatattctca tctccacatc
     9421 taccgaatcc tgttctatat tgacctcaat gggaacaacc ctgaaaaaaa gatagccctg
     9481 atccttgtaa atctgggaaa cgtttgattg ctgcagtttc ccgccgccaa acaacctttc
     9541 ctccatggta accacatcat acacatctcc ctttccgatc ctgaggactg agttcagcat
     9601 ttctccggtg tattgtgtgt ttcctgtcca tgttatattc cggaagtaat attttttccc
     9661 ttcatctata tggatatcta ttcccagatt gcccggatcg ataaaatata tggaatcttt
     9721 tgttatgcgt gcatcacggt atccaagttc atggaatttc tgcaacagga gcgtcttgtc
     9781 gttctggaat tccttttcgt cgaatttctt gctgaaaaag aagttgtaca accttttgtc
     9841 ctttgtcttc ttcatagcaa acctcatctt caggagtgac aactgatcac tcccaaggaa
     9901 attgatctgc tttatgcgta ctttttgatt acgttccaca tgaaacgtaa cctgtacagc
     9961 atttttgatg gtcgtatcct cttggtgagt tgccgttacc cgggcattga gaaaaccttt
    10021 ttcaacaaag tatttcctga tgatatcact ggaggatttt acgacatatt ctgacaattc
    10081 actgcccctt cgtaacctga cacgttcttc gatatcagtc tgctctccct tcttcacacc
    10141 atcgtacttc cattgagata cccggggacg ttccgttatg ctgatattca aataggcatt
    10201 gttgttcacc aggctgtcta catatacggc cacatcggag aaaaaacgct gcataaacag
    10261 gcgtttgact atggttttga gttcatcgcc aggaatactg atagtgtcac ccaaagccag
    10321 gccggtcagg gaaaggatct gatccgcatt cagataattt acaccggtta cctgcaaacc
    10381 ggcaatgaca catttcgtgg ggctggtata atcaataacc acagagctct gtttcctgcc
    10441 tgtggtatca gtagtagcgg cttgttccgt tacctgggca aaaattggta ataccaacaa
    10501 agaaaaggtg atgcagaaaa acaggtgctt cattcaaaag aaattatata gattcttcaa
    10561 tctttccaaa ccgtcttttt ctgttactgt aagcgtttaa ggcagcttcg aagtctggtt
    10621 tgcgaaaatc gggccaaagt acgggagtaa aatacaattc tgtataggcc atttgccata
    10681 gcatgaaatt gcttatgcgc atttctccgc tggtccggat catcagatcg ggttcgggca
    10741 ctccggccgt ggaaaggtaa gaggagaata cttccgggga aagcaactgc tctgcaggcg
    10801 gattttcctc cagcagacgt tttacagcct gaagaatatc ccacttcccg ctgtaattta
    10861 ccgccacaat aagggtcatt cctgtgttgc ctgctgtctg gcgcatacag tcatccatac
    10921 gcttcagggc tttattatca agctgttcgc gatcacctat gaaaacacaa cgtatgttgt
    10981 ttttcatcat cttcggcgtt tcattgtgaa tggcgttcac aatcacagac atgattgcat
    11041 cggtttcgct ttttggacgt ccccagtttt cttccgaaaa agcaaaaagg ctcagataag
    11101 gaataccttt ttctgcggca tattcagtgg ctgcacgaac ggcttctact ccctggcggt
    11161 gtcctacatt cctttccaga ccccgtgaca aggcccatct cccgttccca tccataatga
    11221 tacagacatg gtccggcagt ttctctattt gcttaaccat aattttgacc tccatctctc
    11281 ttatcaaagc ccatcatcag tttctccctg agcgtagtaa caaagtcatt cccttcaaag
    11341 gtcagactgg ttatgctgaa ccgggccttt tttacgataa aactgtaagg caggtccaca
    11401 atctcggacc ggttgtccaa gtgcaactgc gcattgggcg tgcggctcct tacctggatt
    11461 gcaaggattg cgttgtcagg aaaaacgatg ggtctcacat taaggttgtg aggagcaatg
    11521 ggagatagta tgagcacccc ggatccggga aaaacaattg gtcccccaac gctcagggaa
    11581 taagctgtgg atcccgttgg tgtggcaaca atgataccat cgccgcgata agtagaaatc
    11641 tggctgccat ttattttaac atcgacttct atcattgccg aactaagtct ttggatgcag
    11701 atttcattga tggcgtaagg atagatccgc ttctgcagat cttcccgttc tatggtaagc
    11761 acggtatggc ttgttgtgga aaattgcttg ttcaaaacgg catcaaaaag atacccgata
    11821 aaatcggggc cacatccggt cagaaatccc atccttccaa aattcacacc tgccacaggt
    11881 acaccggaat ccttgactat ttccagtgct tccagaaacg taccatctcc ccctacggta
    11941 accagaagat cggttccggc aggtaaaatc ttgtcttgcc ccaacatctg gaaagaaatg
    12001 tctttccttt cgggcaggtg aaagtccttt ttcaaatcct taccgtaaaa aacaactttc
    12061 atatttgtat tgccgataag ttggtttact tttgcaacaa agataaccaa taatgacaaa
    12121 actgagtgtc aacataaata aattcgccac ccttcgcaat gcacggggtg gaaatatgcc
    12181 ggatgtactg aaagccgcta ttgattgtga acgatttgga gcacagggaa ttactgtcca
    12241 cccaagaccg gacgagcgtc atatccggta ccaggatgca cgggacataa aacccattat
    12301 aaccacagag ttcaatattg aaggaaatcc aataccctca ttcatacaat tggtattgga
    12361 ggtcaggccc gatcaggtaa cccttgtgcc cgacgcacac gatgccatta cttccaacgc
    12421 gggatgggac acaatacgtc acgcaagcga actcagggaa tgggtaggcc ttttcaggaa
    12481 tgcaggcata aggacatccc tgtttgtaga tcccaatccg gatatggtac gcgcggccgc
    12541 cctaacagga actgaccgtg tggagctcta tacagaggct tatgcccgga tgtatgacgc
    12601 tgacagggaa aaagccattg caccatatat tgccgcagca gaggcagcgg tcgactgttc
    12661 cctgggattg aatgccggac atgacctgaa cctgaccaac ataagctatt tcaacaaaaa
    12721 catcccgggt ctgctggagg tctcgatcgg gcatgctctc atatgtgaat ccatttacct
    12781 gggtctggag aacaccattc agctgtattt acggcatctc tctaattttt aattatattt
    12841 acagttctta tattttacat tatgaagaag tcatcactga taattactat cttactggca
    12901 ttggccattg ccgcactcta tgttttacac tttacgggaa taggtacggg aaagcccgtt
    12961 gcagacaaag acgatatgca gggcagcgaa accctctccg gaagtatcgt atatatacgt
    13021 attgattccc tcctaaatgc ctacgatttt ttccttgaac tccaggctga ccttgctgca
    13081 aaagcccgca ttgttaaaga agaccttaac aagaaaggac gtgcctttga gaacgatgtg
    13141 acgaatttcc aagataaagt gcaaaaagga ctcataacaa gagcccaggc cgagacacag
    13201 caagcccagc ttgccgcccg tcaacaagag ttggagcaat atgcccagca aaaacaggtg
    13261 gaactggctg aagagaatca agtcatgctc aaccgggtcc tggacgccct caagactttc
    13321 cttgccgaat accgcttact gaaaaactat gatttgatat tgaccacaga cgggaccagc
    13381 aacaccgtca ttgaagctgt ttcttccatg gatatcacta aagatgtagt ggaaggactg
    13441 aacagggaat actcaaaaag cggaaaatag gcatcagcca tgaaatcggt cacggcgact
    13501 cagtttcatt tcgcaaaaca gacgggatta tatataggaa aagtccggga tgtctatacc
    13561 attgacggac gtatcctggt aatgatagca accgacagga tttcggcctt cgatattgtg
    13621 ttccccaaag gaatcactta taaaggacag atccttaaca caatagcttc gcggttcctg
    13681 gattctaccc atgatatcgt tcccaactgg aagattgctt caccacatcc catggtgact
    13741 gtaggttacc gatgcgaacc atttccggtg gaaatgatcg tacggggata cctgaccggc
    13801 agttcctggc gtacctacaa aagcggccaa cgctccctgt gcggtatccc gcttccggat
    13861 gggatgaggg aacaccaggc tttccctgca cccgtcataa ctcccacaac caaagcaggc
    13921 atcgggtcac atgaccagga catatcagga gaggaaatca tcggccgcgg actggtgagc
    13981 gaagaagatt accgcaagat agaggcattc tccctggccc tctttgaaag agggcagaag
    14041 ctggctgcag aaaaaggttt gatccttgtt gatacaaaat atgaatttgg gaaactgggt
    14101 actcagatct acctgataga cgagatccat acccctgact cgtcccggta tttctatgcg
    14161 gaagactatg agaggaatta ccctctaggc ctgcctccca gacagttatc caaagagttt
    14221 gtacgggaat ggctcatgga aaaagggttt agcggacaac ccggccaaaa gatgcctgaa
    14281 ctaactgata cagtgataca aacaatatca gaacgttata tagaattgtt cgaacacctt
    14341 acaggcacgg cttttgtgcc cctggtgtat gacgatacat tctacgacaa catggaagga
    14401 tgtattcgca atatgttagc ccggttatga aaatccgaaa cattttcgtc attttcctct
    14461 tatttgcctc cctcagcctg tatgcacagg aacaacatac tatccggcac aaggtccgct
    14521 ggatggagac cttgtattct atcgcacgaa aatacaaggt cgaccccaag gacattgccc
    14581 tgctgaacga cttgaagaca ggagagatct cgaggggtca gatactgctc ataccggtac
    14641 ccgatgcagg attggctgag caggaggagg agcctttaca ggacaccctt cttgtggagg
    14701 aggagcatga aatgcctgtt aagtggaaaa ctccctgccg ggactacgtc catcctcctg
    14761 cgtgggaacc cgtggtgtcg gtcattttgc cattcccagg caccatggca ggtgccggtt
    14821 ttgttgaatt ttaccagggt gtgctgctgg ctgctgatag gatgaaaaat cagggtatgc
    14881 caattttgtt acaagtcttt gactggaacg aaaagcccat ggatgaactt ctgtcgggat
    14941 cttcactggg gcagagtaac ctgataatag gaccggtata ctcaaaccag gttggaagca
    15001 cactcgccta cttcaggaac agcgaaacaa agatcgtatc gcccctggac agcaatgccg
    15061 aagtctggac cagtctatat cctaattttt tccaggtgca accccctctc gccgctcaac
    15121 aggaatcatt gctaaggtac ctggagcctc acgatgcaac actatgggtc atatcggaag
    15181 aaggagagga aacggtggca ccgggcatca gggcattgct ggacaaaaac ctcatccaat
    15241 acaggaactt ctcctacgat gtgctcatgg gaagagaagt gacagaagtg ctgagggagc
    15301 tgctgggtgc aaatcccagg aatcagatca tcatcgcttc cctgaacgaa gctttcgtct
    15361 ctgacgccat caggaacctg cacctgcttc tggcttacga taacataccg gtagaattgt
    15421 tcgggttttc ccgttggcgt agttttgaaa ctcttgatct tgccgccctg caccaactcc
    15481 aggttgtact cccgctcacg gcttttgttg attattcaga tgtaggggta aaagcatttg
    15541 tacaagacta ccgttcccgg ttccatggag agccttcaca attctccttt cagggatatg
    15601 atgtggccct gttcttttta aatgccctgt tccaatacgg tcctgatttt gaggattgta
    15661 taagcaacat aagcgtaccg ttacttcaaa acaggtttac ctttgtaaag actttagccg
    15721 gaggggggtt ttccaataca ggaacccgtg ttgtacgcta tcgtcccgat ttcaccattg
    15781 aattacttcc ctgatcagag attgtctttt tcaatatcct taaaatacct caccgtcccg
    15841 gctttcaact ctgctgtagc agcctggtcg catacaatga tggcaccggg atgcatctga
    15901 agcgccgtta tggtccacat atgggacacg gctccttcaa cagcatgacg caaagccctt
    15961 gccttgtttt ccccgctgac caggataagc acctcttctg catccatgat ggttccgatt
    16021 cccacagtca gggctgtttt tggcacgcgc tccatatcat tgtcaaaaaa ccgggcattg
    16081 gccatccggg tttctgatgt cagtgttttg attctggtcc tggaagccag tgaggacccg
    16141 ggttcattaa aggcaatgtg cccgtccacg cctacaccac ccatgaaaag acgtatacct
    16201 cccgtgcgct tgatggcctc ttcgtagttc ctgcactcag caaccggatc tgtcgcattc
    16261 ccgtcaggaa cgtgggtgtt cacgggattg atatcaatat gcctgaaaaa attttcctgc
    16321 atgaaataat ggtaggattg tggatgatcc ttaggaatgc ccatgtattc atccatgttg
    16381 aaggtcacga catttttaaa agaaactttt cccgctttat gcatggctat gagctctttg
    16441 taggtgccaa ggggtgagga gcctgtagga agccccagta caaacggatt gtcatgggtc
    16501 ggtttgtagg tattgatctt ttctgctata tacctggctg cccagacaga aagacgatcg
    16561 taattttcca gtatcagtac tctcatgata tattatttaa caactctttg gcattttgta
    16621 tggctgcggg ggtaatggca gacccgctca acattctggc cacttccatg accctttcat
    16681 ctccatgcag gggtagtacc ctggtttcag taacatgttc cacaacctcc tttctgacca
    16741 ggtaatgcgt gctccccttg gcagcgacct ggggcagatg ggtaatggcc agtacctgca
    16801 tgtattcaga caactggtat atcatatttc ccatgcggtc tgctatcttg ccggaaacac
    16861 ccaggtccac ctcatcaaag attatggtgc tcaatcccga gtgttgtgct atgactgact
    16921 tgatgcataa catcagccgc gacatttcac cgcccgaagc tatgcagctc aattccctgg
    16981 gcatcatatc cctgttagcc gaaaacataa tttccatgcc ggtgttgccc aacagtccat
    17041 aggtggcttt ttcccttata acaaactcta tctgtgcatc gggaatgccc aaaagggcaa
    17101 gccgggtgca taactggcgg gacagatcgt caaggcaggc catcctccct ttgtgcaagt
    17161 ctgttgccag ctggtccctg atggcaatgg cagcttccag ttctttttca agcttctcca
    17221 ggcgcgtggt atcgttcacc gtttcttcca gttgtcttcc gaaatctttc tccagtatca
    17281 gcagatcttc caccgtccga acgttgtgtt taccctgcag ggtatagatg gtgtccagcc
    17341 ttccggaaac ttcttccata cggacgggat cgaccctgac ctgttgcagc gaacgggcac
    17401 attctccggc aaggtctttc aattctatcc tggcttgttc catccggtca gagacatcag
    17461 aaaaaccggg atttttctcc gttatttgag ccgaaaccct aactgcttcc tttagaccgg
    17521 taagcaccgg aaaatcacct tcttccaata aagaagagag ctggaacaaa tattgcctga
    17581 tctctgctgc atgttccagc aggcttaatt ctttttcaag ctcttcctgt tctccgggag
    17641 acagtttggc cttctgcagt tcctggtatt ggaaagacag gtaatcggtt tcattctttg
    17701 cctgatcaca ggcatcacgt aacgtacgta ccctggcggc aagggaagtg acctcggcat
    17761 gctttttacg gtattcatcg gcaagctgtg tctgtcctgc aaaggcatcg gctaccctga
    17821 ccggataatc ggtatcggcc aggagcaggc tttcgtgctg ggaatgaata tcgatcaggg
    17881 cattacccaa agacctgaga aatgcaacgt tgacaggctg gtcattgaca aaggaacggc
    17941 tttttccatt agggtatatc acgcgccgaa tgatcagctc cccggaataa ttgacctcat
    18001 cggcggaaaa aagctgttct aaggcattat cgttaccggg tatcctgaaa caggcctcca
    18061 ccacagcgtt ggagccaggg tccctgagaa ttccggcatc ggccttttgt cccaaaacca
    18121 gtgaaagagc acccaatatg attgatttac cagcaccggt ctccccggta atgatagtca
    18181 gaccctctga aaactcaata tccaggatat tgatcagggc ataattggtt atgctcagcg
    18241 agtaaagcaa acttattctt taggttcttc aaccactttg taatatgttt ctccttcctc
    18301 aaattcgtta atggctagca gtgtgggttt tggctgacgt tcgtaataac gtgaaatctc
    18361 tatctgttca cggttttcaa acgtttcttc cagtgtatct gtgaaatttg aaaattcttc
    18421 caggcggctg ttcaattctg acttgacctc tgaggcaatc tggtttgccc tttgcgcaat
    18481 gatcattacg gtctgataaa tgttacctgt cttttcagaa aatttggaaa gatctcgggt
    18541 aacagttgta tcgggaatag gttgtttctt gctttccata tagtgttaaa attattgttt
    18601 agttgtgaat tgctgggctt tgcggaacat gttgtccact tccctgcggt attttgaatc
    18661 gggaaattcg ctgacaaaat tatagtactc atctataaca ttgaggaaac gcccccgttg
    18721 cagttcgggc aggctgttgt tggagtactg gtaattagcg gctacaatgt aataaaggat
    18781 ttcttcacgg tacatgcttt cgggattttc cttcagggtc tccttgaacg catgcgttgc
    18841 cgccttgtaa tcttctatgg tataataaag ccgggccgat tcaaacgcct tgcggtcaag
    18901 ccgctggtag agatccccga gcatcttcct gcacacttcc tggtattcgc ttccgggata
    18961 atcatagatg aattcctcaa tggcagcaat ggcaacccgg cttggttgct gatccagttc
    19021 atagcggtat gtgttttgat acaggcaatc cagtctcatg aagcgggccg actttgtaaa
    19081 cggactgcgc ggaaagattt ctacaaactg tttgaaatta tcttcggcaa ccagaaaatt
    19141 gttcaggtgg taattgctca gccccaggta atactgtaat gtgtcatcct ggaccgttcc
    19201 cctgtacacc atttgcaact gatctagaag aggctgtgat cttgaatatt tttttgcctc
    19261 gaagtattct atggctttcc tgaatttcag gtcgtaatct gtactgcgca gtaacacctc
    19321 atattggttg acacaggccg gtgcaaccgt aagcaaaagg aggacaaagg ttaagaaatg
    19381 ttttttcatc aagatcatca attgcaaata tacgacagtt tagcaaatta caggccatat
    19441 tgcatagaca tataacgttc caggtcgata gccgccatgc aaccgctcgc tgctgctgta
    19501 atcgcctgcc ggtatttgga atcctgcacg tcacccgctg caaagactcc cttcacattg
    19561 gtttccgtac tttttggctg cgttatgatg tacccttcgg gattgacttc cacccagggc
    19621 aggaaaggct ctgaattggg atgatgcccg attgccagga aaaatccagg tacattgatg
    19681 attctctcac gaccggattt atggacaaga cgcgctcctg tgacacctga gtcgtctccc
    19741 agtatttcag ccgtctggtg ctcccagagt atttcaatgt ttggggtgga caggaccctt
    19801 ttttgcatca cctgggatgc tctgagtacg ttgcgccgta tgatcatata gactttgcgg
    19861 cacaaagcag aaagatatat tgcctcttca caggcagtat cccctccgcc cactaccgcc
    19921 acatctttac ccctgtaaaa gaatccgtcg catgttgcac aggccgagac gcccatgccc
    19981 ttgaatttct gttccgattc cagccccagg tagcgtgccg tagcacccgt agcaatgatc
    20041 aaagcttgtg tcagcacctc tgtcccgtta tcgaacctga tacggtaagg aggttcgctc
    20101 aggttggttt cagtgacgat ccctgtcctg aaatcagtgc caaatcgcaa agcctgtttg
    20161 cgaatgtctt ccatgagctg gctgccctgg acaccctccg gatacccggg aaaattctcg
    20221 atctctgttg tggttgtcaa ctgtccgcct ctcaacactc cctcataaag aaccggctgg
    20281 aggttggccc tggcggcata tatggcagct gtgtaaccag caggaccgct tccggcaata
    20341 aggcatttaa tggtttccat agtgttattt ttcctataaa tttacaaatt tcaaaaaaaa
    20401 ccgtatcttt gtcgctctga aaaagcgggg cgtggcgcag ttggctagcg cacttgcatg
    20461 gggtgcaagg ggtcgaatgt tcgagtcatt tcgccccgac tttttttatc agccg
//
LOCUS       DAOSOF010000016        19953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2705_length_19953_cov_4.909488, whole genome shotgun sequence.
ACCESSION   DAOSOF010000016 DAOSOF010000000
VERSION     DAOSOF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19953
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2705_length_19953_cov_4.909488"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2544
                     /locus_tag="PLM68_01795"
     CDS             <1..2544
                     /locus_tag="PLM68_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54137.1"
                     /translation="AGTGLLIHHTLKALTMDSNIAMELFKIDEITWYTVIAYLVVSLL
                     FLAFFLLFKTLQKTANLPYLRLLAGQRFTYLFVFLAALYSILTVSIYGREKEFNLYQV
                     WTQKMSLERDLDLEMQLRRVEERIAIDPILSIMIQMEQDHDLILSRIKETYFSNFLQR
                     YQIQITICEPGDVLVVAGMPRPVSCYQFFHSDILRNGTPLAENSKFYNIDSKLGNISY
                     IGVFTFYNYSGSQDLYIELDSRYIKDALGYPSLLSDRSQFDRFKLPYDYSYAKYIDGH
                     LVSNSGKHVYPSKLTYEPEDGFSLRKSERSLHCYYKTAGKVLIITRPQRSPLPYLVSL
                     SYLFLFYGFVFALALALLRENDLKINLPGNSFRRKITVLLVGLLFVALVLMAIGSIGF
                     GLKLYEETNTQQMQDQMDAARSSLEEYVRYADRSNDPAGNVVELMNTINRLSSNININ
                     VNLYDPQGRLMRSSQPEIFERHLLGARMDSEAFRQLHEYNNPQFTHKEQIGSLVYYSM
                     YAPLFNTNGELAAYINVPYLSGQTNVSRDTSAIVAAIINLYLLLLLAAVFVSYTFSNQ
                     LTRPLAELVEKISRLDVTRKPEHIHYTSEDELGVLVRAYNKMADNLNESTRHLAQSER
                     EQAWREMARQIAHEIKNPLTPMRLSIQHLIRLKQEGGPRWLEHFDKIVASLLEQIDIL
                     SKAASEFSSFSRINTEEPVEVDLNGLINEQIVLFTSFEHIKIHFESEVSQPLVTVRRS
                     QWVSVIINLLSNAIQSVEEQTIGVISIHLAEEGDSYLISVADNGPGVAPENQHKLFKP
                     NFTTKSGGTGLGLAICRGIVEQYRGEIFYTSSEWGGACFNVRIPKKPVI"
     gene            complement(2537..3706)
                     /locus_tag="PLM68_01800"
     CDS             complement(2537..3706)
                     /locus_tag="PLM68_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT54138.1"
                     /translation="MRILIIDDEKSIRNTLKEILEYEGYEVALAENGEQALSVLEQGK
                     VQIDTIFCDIKMPGIDGIEVLEKIQDMELDVPVIMISGHGDIDTAVECIKKGAYDFIE
                     KPLDLNRILITIRNANDRTTLVQENKKLKRKEDKVPPLVGKSPAISRIWNIIERVAST
                     DARVLITGSNGTGKELVARWLHHKSNRSRMPFIEVNCAAIPGELIESELFGHEKGSFT
                     SAIRQHKGKFEQADGGTLFLDEIGDMSLAAQSKVLRVLQENRITRVGSDKDITVSVRV
                     VAATNKDLVQEIGKGTFREDLYHRLSVILIHVPPLAERREDIPLLTEYFIEQICSEYG
                     MSKRSITPQAVEELQKLNWTGNVRELRNVIERLIILSPGTIGEEEVRAYADPLFI"
     gene            3783..4484
                     /gene="cmk"
                     /locus_tag="PLM68_01805"
     CDS             3783..4484
                     /gene="cmk"
                     /locus_tag="PLM68_01805"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT54139.1"
                     /translation="MKQMIIAIDGHSSTGKSTFAKAIAKAMDYTYLDSGALYRAVTLY
                     ALEEGLTDSQGLPDASLLTERLRDITVSFCRNPATGLSETYLNGKNVEERIRSLDVSR
                     SVSPVSVIPRVREFVDNELRKWGEKKGVVIDGRDIGTAVFPQAELKIFMTAPAHVRAR
                     RRLDEMKAKGEEATFEEVLDNVLSRDHLDSTREVHPLTRASDAVLLDNGNMSVEEQME
                     WFKKIMEERWGIRLK"
     gene            4463..5344
                     /locus_tag="PLM68_01810"
     CDS             4463..5344
                     /locus_tag="PLM68_01810"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT54140.1"
                     /translation="MGYQVEIDKKSGFCSGVIRAIRCTETALGENKPVYSLGAIVHNN
                     EELHRLEAKGLRVINRGDLEHIPEGSTILIRAHGEPPSTYALMAERKIRLLECTCPVV
                     LLLQKKIKKEHARIKPQNGQIIIFGKQGHAEVDGLVGQVGGDALVVENEKDLEGLAFS
                     GGPVSLFSQTTQNPEGYQKIREYLAKLLEEKGIDPGMLKVFDTICSQVDGRQAALSRF
                     ALSRSVILFVAGKESSNGKVLFRTCKEANPRSHVIENPGEISPEWFRPGDTVGICGAT
                     STPFWLMEQVAEKVRNL"
     gene            5344..6972
                     /locus_tag="PLM68_01815"
     CDS             5344..6972
                     /locus_tag="PLM68_01815"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54141.1"
                     /translation="MKEKYADILFPLALENLLTYRIPESMHSQVETGCQVIAPVGHSI
                     HPGVIRRIRDEPPEGSLPESIRDLVPAGITLPLIPEKTLLFWEWIARYYMCPPGLVAK
                     MALNLWNFKRRATSTSAVSGPLPEPGPPLYLQRAEPGTSYREYVRRVLDLGGQCLVVC
                     PDRFSCESLHEAMLPAFGEMAICFHSKRPLREQSKAKKELYAGNPCVVTGMHLSLLLP
                     FTRLGLVIVDREEHPSHKKSDAVPFLQARDAALVLGRQFHAQVILGSSMPSLETFYNI
                     EKGKFGIPESGPALIPLQDKPLLIDTSRSLTTGSLYDRLEVRTRAAVKQCLEEQKKVI
                     LAEADPYCPEDLPQEPGIIVCRPLQLHHHLDSATGLICFLHMEKLLARKHFRATEQTC
                     HLVENALRWAAAQTFRVPVIVQTSCLDHPFYQFLNNGFDVSCLTNMLQERKKYGYPPY
                     TRQLLITVFHNRKSLARDRINIFYSVLQSASLPVRTDGPFVPSEVHALMFSYRLQVTI
                     PRTVNAQQVKDRILSLIRETPLAPARIRIDVDPA"
     gene            complement(6979..8766)
                     /locus_tag="PLM68_01820"
     CDS             complement(6979..8766)
                     /locus_tag="PLM68_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT54142.1"
                     /translation="MKSKFLILLILSLTWFVTSSSAQIMRRPTKKQLTQQVDSLLRLT
                     DSLQRMLLQAELPETDTSFYYNGINEGGFDETETYPDFDYDSLSTEQRLSLYYHQNQL
                     NQMPDSHTLETLDTALLTSAIPDSVYIDRLTRMNSFIKLPYNNIVRNHIIHYTQKMPD
                     RISTILGLAQYYMPLFEEIFDSYGMPLELKAMAVIESALNPRAVSRARATGMWQFMYR
                     TARNYNLTINSYVDERLDPVVSGDAAARYLLDSYRIYGDWILAIASYNCGVGNVNKAI
                     RRAGGSREFWDIYPFLPRETRGYVPAFVAALYTLRYYPEHQIRPLPVEMPAHVDTFEV
                     NRMLHFEQITNYLNISKDQLRDLNPQYVKDIIPGVERPYILRLPFEHTGDFAALQDSI
                     YSFKDSVYFNPLIMNTRATASDTQITHRVLSGETLSHIAYRYGVRIADLKYWNGLRSD
                     RIVANQRLIIYTSRTGAPRTETAAAATPSAEPSRTRGNLISHKVQKGETLGGIAEKYK
                     VSAANLRSWNNLRNNTIYAGSTLKVYTSATTTTSQDGSFEYYTVKSGDNLWDIANRLG
                     ITVQNIRALNGLDSESRIYPGQKLKIRKI"
     gene            complement(8783..9667)
                     /locus_tag="PLM68_01825"
     CDS             complement(8783..9667)
                     /locus_tag="PLM68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOT54143.1"
                     /translation="MKRSLIFILCLLCSGSLVYGQFAVDMGQKDTKSSQDTTVQAKEP
                     FKLRTYFSALAGKDTLKLGRVFSGAVVLPGYSQAYNRQYWKIPVVYAGMGAGIFMGTR
                     FHNKYKETENDSYALYQNLCYVGAGLFYWGSLLDGVAGFKHEKAFLPAKATLYSALLP
                     GLGQVYNGDYWKIPVIYGGLIACGYFWQYNGLQYNRYKTMYNHAMAKPSEYDGWMTPE
                     NLKWYRDTFRRYRDYSIIATVLVYALNVIDANVFAHFRDFDISDDLTLRVEPGLIPVA
                     TYASAGYPGAGIQMRLTF"
     gene            complement(9682..10578)
                     /locus_tag="PLM68_01830"
     CDS             complement(9682..10578)
                     /locus_tag="PLM68_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT54144.1"
                     /translation="MTKKPALGKGLGALISETPSLSDWKPRNPVVLPQVEGTSVIDID
                     LIEVNPEQPRKEFNGEALAELAASIRNLGLIQPLTLRKLAVGKYQLVSGERRLRAARM
                     AGLTKVPAYIRQVEDNALLEMALVENIQREDLDAMEIAYGFQRLTEECDLTQEQLAER
                     VGKKRATVANYLRLIQLPAQIQLFIRAGALTMGHARALLSLEEQDARLALAKKIVEKG
                     LSVRQVEAMVRPAGPEKNETQAREAELPDVYYRVLERIGTYFNNNISLKRNARGEGVL
                     TVYCSSDSEMEAFLEALEKNNI"
     gene            complement(10571..11344)
                     /locus_tag="PLM68_01835"
     CDS             complement(10571..11344)
                     /locus_tag="PLM68_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT54145.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLAASLAVMEKNVLLVDADPQAN
                     TTSGLCYDPNASSGDTLYECLIGKMPIEKTIIQTEIQGLKLIPSHIDLVGAEIELLPV
                     PEREGVLKSHLDTVSHHFDYILIDCSPSLGLITVNALTAADSVIIPVQCEFFSLEGLG
                     KLLNTIKIVQNRLNPALAIEGFLLTMYDVRLRSHNQIVEEIRHHFQEMVFQTVIQRNV
                     RLSEAPSHGKPVILYDAASVGSNNYLNLAREFLKKNQHD"
     gene            11435..12406
                     /locus_tag="PLM68_01840"
     CDS             11435..12406
                     /locus_tag="PLM68_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOT54146.1"
                     /translation="MSLTQWLIILNPVAGGGKTARMWPHISKYFLERDFIFECVFTKH
                     KNHAVTIVIEAIREGFRKFIVIGGDGTLHEVINGIFFQQDVPTSDITIGVIPIGTGND
                     TVSPYGIPLEYRQAMDIICNNKSVLTDVGTVTFQDYGVERKRFFINSAGLGLDAEVVR
                     HYEKVVEQHHDRGKSLYLQSLLNTFMFYRSKDYRILLDGEVFYEGPVLTLNIGIGTTI
                     GSGMKALPLAIPDDGLFDITLVTSMSKLSLALKIKDFINGKVYSFKEAMHGRGRTVEV
                     LPNSKQATGLETDGELMGSPPYVFNIIPSSIRIFAGESFVPFVRNET"
     gene            complement(12390..14297)
                     /locus_tag="PLM68_01845"
     CDS             complement(12390..14297)
                     /locus_tag="PLM68_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54147.1"
                     /translation="MRNVLWVLLCTAMICHAQAQDRDVMLENLAREYEFTGQDLEEML
                     DERISLNTATGEDLRLIGLLSPYQVASLIDYRQRYGAIISWAEVFLIPGFTEQDTRLL
                     SLFFHLEPEATYDRLQLKSMFRHGKRSFLVQTRTFFPRGEEYSPITEAEYQARPNSRY
                     QGVPWYRYLRYDYRYRDRVRWGFTLESDPGEKQVVDFLSFHAEIRDVKPFRTLVIGDF
                     RARFGQGLLLWNGTQFGKASSTASLCKQEMGITPYRSRDENLFFRGLGASFRRRSLDL
                     SVFISYRTLDARIVEEGFTSLVETGYHRTPLEHEKKNTLRSWVTGLNLSWSAERWKAG
                     VTALGYGYNHPDAGRITYYNDYKNRTVPFGGIAADLSFRLPSWRFFSEAALDLGGSPA
                     ALAGIIYYGKNGNQAGMQLRYFTPSYTAAYSARLGRNADPSNEYSLQMTGTLVLPCAW
                     KMDLSAWAYFFPRARYLCHDPSYGWDYRLQVHRDHHMLLFRQQRSLSDKGTLDRQSLC
                     LRTAVRFSDAFQLALRGDGMYCLLLENSSEWGGTAYAQLEYENVKETFRLSFRLALFH
                     TESWESRIYIYEPDVLYGYSVPALYGKGIRACLNLTYSPFPATDIWFKVSAMYKESYV
                     CESKVQIRFRF"
     gene            complement(14353..14862)
                     /gene="purE"
                     /locus_tag="PLM68_01850"
     CDS             complement(14353..14862)
                     /gene="purE"
                     /locus_tag="PLM68_01850"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT54148.1"
                     /translation="MNPQVSIIMGSISDMSVMEEAARFLNQMEIPFEIKALSAHRTPD
                     KVAEYAQEASQRGIKVIIAGAGGAAHLPGIIAAYTSIPVIGVPVKSAKSLEGLDSILS
                     ILQMPAGIPVATMALDGARNAAIFAARILALSDSNLAERMIDFQHNLNLKIEEANESI
                     KEIKYRFKI"
     gene            14909..15445
                     /gene="hpt"
                     /locus_tag="PLM68_01855"
     CDS             14909..15445
                     /gene="hpt"
                     /locus_tag="PLM68_01855"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT54149.1"
                     /translation="MERITLQDKSFRTYIPYQEIDKAIEQVAAALNRDLRNEDEPVFI
                     GILNGSFMFTADLMKKIDLSCVVTFVKLSSYSGTSSTGTVREIVGLCTDITDRTVVVV
                     EDVVDSGQTIEKLVEDLKKKNPRKIIICTLLFKPNAYRGKIPIDYVCIRIPNDFVVGY
                     GLDYNNLGRQLKDLYVID"
     gene            15449..16024
                     /locus_tag="PLM68_01860"
     CDS             15449..16024
                     /locus_tag="PLM68_01860"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT54150.1"
                     /translation="MLNIIIAGPPGSGKGTLAKLMIEEFGFVHLSTGDMLRHEIGLGS
                     ALGKEVEAILARGDLVSDQIVISLIANNLKANPGARGFLFDGFPRTLPQAEALDDFLE
                     KSGHPLQLMILLKVDDQVCEMRLLRRAGIENRPDDMDPERVRHRLATYYAQTVPIIDH
                     YKKQGKFVGVDSSKTPEYTLNQVSECLKQIL"
     gene            16003..16998
                     /gene="obgE"
                     /locus_tag="PLM68_01865"
     CDS             16003..16998
                     /gene="obgE"
                     /locus_tag="PLM68_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT54151.1"
                     /translation="MSETNFVDYIKLFCASGKGGAGSVHLLRAKYVPKGGPDGGDGGR
                     GGHIILKVNPQFWTLIHLKYRKHIRAEHGEAGRGNLAHGADGKDVILEVPAGTVVKNA
                     EDEQILAELTQPGQEFILCKGGRGGLGNTHFKSPTHQTPRFAQPGEAGQEGWFILELK
                     LLADVGLVGFPNSGKSTLLSAVSAARPKIADYPFTTLEPQLGIVSLPDHRSFVMADIP
                     GIIEGAHQGRGLGLRFLRHIERNSMLLFMVPADCPDVTAQYRILLKELGKYKPELLDK
                     QRMLAVTKADLLDPGGEKMLRRRLPRSLPVLLISSITGYNISQLKEQIWNILWTQ"
     gene            16986..17558
                     /locus_tag="PLM68_01870"
     CDS             16986..17558
                     /locus_tag="PLM68_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT54152.1"
                     /translation="MDSIIELDHKLFFILNGAHSDFLDPVMKTLSNIPVWIPLYLLIA
                     GGFFLVLPWRQALVAILGILFTFLLTDQLSSTLIKEWVHRLRPSHIPEWEGTFRLLED
                     KGGLYSFVSSHASNVFGLACFTSLVYRKKWYTCGIFVWAALVAYSRIYVGKHFPLDVV
                     CGALIGLLLGWAVYRLYRRIGRKIQTKCAA"
     gene            17546..18532
                     /locus_tag="PLM68_01875"
     CDS             17546..18532
                     /locus_tag="PLM68_01875"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOT54153.1"
                     /translation="MRCIIDSTTDPYWNLAVEEYLLTVPEEPVFRLWRNAPAIIVGRN
                     QNTPDQINSEYVREQGIAVVRRLSGGGAVFHDLGNINFTFVDAYVPRTDPGEAFARFT
                     RPILDVLHSLGVAAELEGRNDLVIEGRKFSGNAIALYKNRILMHGTLLFSASIRDLSL
                     ALQVKARERGRGVASNPRRVCNLSEYLPKGMKTEDFLEVLKRHIAGSPDKQTREQPLD
                     PRECQIIETLCRKKYRTYAWNYGRAPLRGDLRRERFAGGNVEILLQVNNGIMEEVKIY
                     GDYFFLYPTEEIEQALQGCPHNREAIAGRLARFSLDDYFRDIPREGLLDLFI"
     gene            complement(18521..>19953)
                     /locus_tag="PLM68_01880"
     CDS             complement(18521..>19953)
                     /locus_tag="PLM68_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT54154.1"
                     /translation="AGATSEIEMAYTLADGLEYLRTGVKAGLDIDDFAPRISFFWAIG
                     MNHFEEIAKMRAARMIWAKIVKSFNPKNPKSLSLRTHCQTSGWSLTEQDPFNNVARTC
                     TEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQLYLQDETEICRGIDPWAGSY
                     YVETLTHQMAHKAWELIQEIEDLGGMAKAIETGIPKLRIEEAAARTQARIDSGEQVVV
                     GVNKYRLEKEDPIDILDVDNTKVRLSQIARLEELKKTRDSKKVAASLAALTRAAADNS
                     GNLLELAVEAARNRATLGEISDACEKVAGRYKAVIRMNKGVYLSEVKDDPYFAKAREM
                     VASFAAREGRQPRIMVAKLGQDGHDRGAKVVATGYADIGFDVDMGPLFQTPEEAARQA
                     VENDVHIVGVSSLAAGHKTLVPQVVKTLREMGREDIMVIVGGVIPGQDYQELYDAGAV
                     AVFGPGTPVSLCAIRMLELLEQQSDK"
BASE COUNT         4942 a         5015 c         4964 g         5032 t
ORIGIN      
        1 gcaggtacag gcttgctgat acaccatacg ttgaaagccc tgactatgga ttccaatatt
       61 gccatggagc ttttcaagat tgatgagatc acctggtata cagtgatcgc ctacctggtc
      121 gtgtccttgc tgttccttgc ttttttcctg ttattcaaaa ccttgcagaa aacagccaac
      181 ttaccatact tacgcttgct tgccgggcaa cggttcacct acctgtttgt ttttctggcc
      241 gcactataca gtatacttac agtcagtatc tacggcaggg aaaaggaatt caacctgtac
      301 caggtatgga cccagaaaat gtcgctggag agagaccttg acctggagat gcagttgcgc
      361 cgtgttgaag aaaggattgc catagacccg atcctgtcca taatgattca gatggaacag
      421 gatcatgatc tcatcctttc ccgcataaaa gagacgtatt tctccaattt cctgcagagg
      481 taccagatcc agatcacaat ctgtgaaccg ggtgatgtcc tggtggtagc cggtatgccc
      541 cggcctgttt cgtgctacca gttttttcat agcgatattc ttagaaacgg gacccccctg
      601 gcagagaact ccaagtttta caacatagac agcaagttgg gaaatataag ttacataggg
      661 gtttttactt tttacaacta tagcggttcc caggatcttt acatagaact tgattcacgg
      721 tatatcaagg atgcactggg atacccgtca ctgctatcgg acaggtccca gttcgaccgg
      781 tttaaattgc cctacgatta ttcctacgcc aaatacattg acggacatct ggtttctaac
      841 agtggtaaac atgtgtatcc gagcaagttg acgtatgagc ctgaggatgg tttcagcctt
      901 cgcaaaagcg aaaggagcct tcactgttat tataagacag caggcaaggt gctgatcata
      961 acaaggccac agcgctcacc ccttccctac ctggtctccc tgtcttacct attcttgttt
     1021 tacggattcg tctttgcact tgccctggcg ttgctcaggg aaaatgatct gaaaatcaat
     1081 cttccgggca attcattccg gcgcaagatc actgtgttgc tggtggggct cctttttgtg
     1141 gccctcgtat tgatggctat cggcagtatc ggttttggtc tgaaacttta tgaggaaaca
     1201 aacacacaac agatgcagga ccagatggac gctgccagat catcgctgga agaatacgta
     1261 cgttatgctg accgttccaa cgatcctgcc ggaaatgtgg tggaactgat gaataccatc
     1321 aaccgccttt cctccaacat aaatatcaat gtgaatttgt acgatcccca gggaaggctg
     1381 atgcgttcct cccagccgga aatattcgag cgccatttgc tgggagcccg tatggacagt
     1441 gaggcgttcc ggcaactcca tgagtataac aatcctcaat tcactcataa agaacagatt
     1501 ggatctttgg tttattattc catgtatgcc ccgctgttca acacaaacgg ggaacttgca
     1561 gcctatatca atgtcccata cctctccgga cagaccaacg tgtccaggga tacttccgcc
     1621 attgttgcgg ccatcatcaa cctgtacctt cttttactgc tggcagccgt cttcgtgagt
     1681 tatacctttt ccaatcagct cacaaggccc ctggcggaac tggtagaaaa aatcagccgg
     1741 cttgatgtga cgcgcaagcc ggaacacatc cattatacat ctgaagatga actgggtgtc
     1801 ctggtcaggg catacaacaa gatggcggat aatctgaatg aaagtacccg gcaccttgct
     1861 caatcggaaa gagaacaggc atggagggaa atggctcgtc agatcgccca tgagatcaaa
     1921 aacccgctaa cccccatgcg tctcagcatt caacacctga tccggctgaa gcaggagggt
     1981 ggccccagat ggctggaaca ctttgacaaa attgtggctt ccctgctgga acagattgac
     2041 atcctgtcca aagcggcctc tgaattttcc agcttttccc gtatcaatac tgaggaaccg
     2101 gtagaagttg acctcaacgg gctgatcaat gaacagatcg ttttgtttac atcttttgag
     2161 cacattaaga tccatttcga atctgaagta agccagcccc tggtaacggt aagacgcagc
     2221 caatgggtga gtgtgataat caatcttttg tcaaatgcca tacagtctgt tgaagaacag
     2281 actattggcg tgatcagtat ccatttggca gaagaaggag attcatacct tatcagcgtt
     2341 gccgacaacg gaccgggtgt ggcccctgaa aaccaacaca aactcttcaa gcctaatttt
     2401 acgaccaaaa gcggcggaac cggtttgggt ctggccattt gccggggaat agtcgaacaa
     2461 tacaggggtg aaattttcta cacctcatcg gaatggggcg gtgcatgctt caatgtaagg
     2521 atccctaaga aacctgtcat atgaacaggg ggtcggcata ggccctgact tcttcctccc
     2581 ctatggttcc tggagaaagt attatcagcc gttcaatcac atttctcaat tcacggacat
     2641 tgcccgtcca gttgagtttc tgcaattcct ccactgcctg gggggtgatg gacctttttg
     2701 acatgccgta ttcggagcag atttgttcaa taaagtattc tgtcagcagc ggaatatcct
     2761 ccctgcgctc agccaatggc ggtacatgta tcagtatcac gctcaggcgg tggtacaggt
     2821 cttcacggaa cgtccctttg ccaatttcct ggaccaggtc cttgttggtg gcagctacca
     2881 ccctaacgct tacagttata tctttgtcgg atcccacacg cgtgatcctg ttttcctgca
     2941 gtacgcgcaa cacttttgac tgtgctgcca gggacatgtc tccgatttcg tccaggaaga
     3001 gtgttccccc atcggcttgt tcgaatttac ccttatgctg tcgaattgca gaagtaaagg
     3061 agcctttttc atgtccaaag agttcgcttt ctatcaattc accgggtatg gcagcacagt
     3121 tcacctcaat gaatggcatc ctggagcggt tgcttttatg gtgaagccag cgggcaacca
     3181 attccttgcc cgttccgttg gaaccggtta tcagcaccct ggcatctgtt gaggccacac
     3241 gttcaataat gttccatatg cggcttattg ccggagactt tcctaccagc gggggcacct
     3301 tatcttcctt ccttttcagt tttttgtttt cctggaccag ggtggtcctg tcgttcgcat
     3361 ttctgatagt gatcagtatg cggttaaggt ccaggggctt ttcaataaaa tcatacgcac
     3421 cttttttgat gcactccaca gccgtatcta tatcaccgtg gcccgagatc atgataaccg
     3481 gcacatcaag ttccatatcc tgaatctttt ccagcacttc aatcccatcg atccccggca
     3541 tttttatgtc gcagaaaatc gtatcaatct gtacttttcc ctgttccaga acggataaag
     3601 cctgctcccc gttctctgcc agggcaactt catatccttc gtactccagt atctctttca
     3661 aggtattgcg tatgcttttt tcgtcatcga tgatcaatat tctcatactc cgcgtgatta
     3721 ttcgtgcaaa gataacccga atttcgcaaa taggagtttt tccgttacat ttgagacaga
     3781 ttatgaagca gatgatcatt gccattgacg ggcattcttc aacgggaaaa agcacttttg
     3841 ccaaagccat agctaaggcc atggactata cctacctgga ttcaggtgca ctctacaggg
     3901 cagtgaccct gtatgcactg gaagaagggc ttacggactc ccagggactg cccgatgcct
     3961 ccctgctgac cgagcgcctg agagacataa cggtttcatt ttgcaggaat cctgcaaccg
     4021 gcctgagtga gacttacctc aacggaaaga acgtagagga acgcatcagg agccttgacg
     4081 tatccagaag tgtcagcccc gtctctgtca ttccccgggt tagggaattt gttgataacg
     4141 aacttagaaa atggggcgaa aaaaaggggg ttgtaattga cggcagggat atcggcacag
     4201 ctgtttttcc ccaggctgag ttaaagatct tcatgacagc tcccgcccat gtccgggccc
     4261 gcaggcgcct ggacgagatg aaggcaaaag gagaggaagc cacttttgaa gaagtgctgg
     4321 ataatgtgct tagtcgcgac cacctggact cgacaaggga agtccatccc cttaccagag
     4381 catcggacgc cgtattgctg gacaacggga acatgagcgt tgaagaacaa atggaatggt
     4441 ttaaaaaaat aatggaagaa cgatggggta tcaggttgaa atagataaaa aatcgggatt
     4501 ctgttcgggt gtgatccgtg ctatccgctg taccgagaca gccctgggtg aaaacaagcc
     4561 tgtttactcc cttggggcta tagtgcacaa caacgaggaa cttcaccgcc tggaggcaaa
     4621 aggcctgcgg gtgattaacc ggggtgacct ggaacatatc ccggaaggat ccaccatact
     4681 catacgcgct cacggggaac cgccttccac ctacgccctg atggctgaaa ggaaaatcag
     4741 gctgctggaa tgcacctgtc cggtggtact actgttgcag aaaaaaatca aaaaggaaca
     4801 tgccaggata aaaccgcaga acggacagat cattatcttt ggcaagcagg gtcacgcaga
     4861 ggtggacggg cttgtgggac aggtaggggg tgatgcactg gtagtagaga acgaaaagga
     4921 tctggagggt cttgcgtttt ccggaggccc cgtgtccctg ttttcccaga cgacccagaa
     4981 cccggaaggg tatcagaaga tccgtgaata cctggcgaag cttttagaag agaagggtat
     5041 cgaccccggc atgctgaagg tgtttgatac catatgctcc caggttgacg gaaggcaggc
     5101 agcattatca cgttttgccc tcagccgctc tgtaatcctg tttgttgcag ggaaagaaag
     5161 ttctaacggc aaggtcctct tccgtacctg taaggaagcc aatccgcgaa gtcacgtcat
     5221 cgaaaatccg ggtgagattt ccccggaatg gttcaggcca ggagatacgg taggtatatg
     5281 cggagccact tccacccctt tctggcttat ggaacaggtt gccgaaaaag tccggaattt
     5341 gtaatgaaag agaagtatgc cgatatcctt tttccacttg cgctggaaaa cctgctgaca
     5401 tacaggattc cggaatccat gcactctcag gtggaaacgg ggtgccaggt tattgcaccc
     5461 gtcggacata gcatccaccc cggggtaatt cgcaggatcc gggatgaacc gccggaagga
     5521 agcctgcccg aaagtatcag ggatttggtt ccggccggga ttaccttgcc cctgataccg
     5581 gaaaaaacgc tcttgttctg ggaatggata gcccgttact acatgtgtcc tccgggtctg
     5641 gtggcaaaaa tggccctgaa cctgtggaat ttcaaaagac gggcaacaag caccagtgcc
     5701 gtgtccggac cacttcccga acccggacct cccctgtacc tgcaaagagc ggaaccggga
     5761 acgtcttaca gagaatatgt ccgccgtgtg cttgacctgg gaggtcaatg tctggttgtt
     5821 tgtcctgacc gtttctcctg tgaatccttg cacgaggcca tgctcccggc attcggggaa
     5881 atggccatct gttttcattc gaaaagaccc ctgagagaac aaagcaaggc caaaaaagag
     5941 ttgtatgcag gcaatccctg tgtggtcacc ggtatgcatc tttcgctcct tttacccttt
     6001 acccgactgg gactcgtgat agtggacagg gaagaacatc cctcccacaa gaaatcagat
     6061 gcagtcccgt tcctgcaggc aagggatgcg gccctggtgt tgggccggca gttccatgca
     6121 caggtcatcc tgggatcttc catgccatcc ctggaaacct tttataacat agaaaaggga
     6181 aaattcggaa tcccggaaag cggccctgcc ctcatcccat tgcaggataa accccttttg
     6241 attgacactt cacgttcatt gaccaccgga tcgttgtacg accggctgga ggtgcgcacg
     6301 cgggcagccg tgaagcaatg cctggaagaa caaaaaaagg tgattcttgc cgaggcggac
     6361 ccctattgcc ccgaagacct tccccaggaa cccggcatca ttgtctgcag gcctttacag
     6421 ctgcaccacc accttgactc ggccacaggg ctgatctgtt tcctgcatat ggaaaaactt
     6481 ctggccagaa agcacttccg ggcaacggaa caaacctgcc acctggtgga aaatgcactc
     6541 cggtgggcag ctgcacaaac gttccgggtt ccggttatcg tgcaaacctc ctgcctggac
     6601 catccgtttt accaattcct caacaatgga tttgatgttt cctgcctgac gaacatgttg
     6661 caggaacgga aaaaatacgg ctatccgcca tatacccggc agctactcat taccgtcttt
     6721 cataacagga aaagcctggc ccgggatagg atcaacatct tctactctgt ccttcaatct
     6781 gcaagtcttc cggtacgaac agacggaccc tttgtaccat cagaagtaca tgccttgatg
     6841 ttctcatatc gtttgcaggt gaccataccc cgtactgtaa atgcacaaca ggtaaaagat
     6901 aggatcctct ccctgatacg ggaaaccccc cttgctccgg cccgcatacg tatagacgtg
     6961 gaccctgcct gacagatatc agatcttacg gattttcaat ttctgtccgg gatatatcct
     7021 ggattcacta tccaatccgt tcaaagcacg aatattctgg actgttatcc ccagcctgtt
     7081 ggcaatatcc caaaggttgt caccagactt cacggtgtaa tactcaaaag acccgtcctg
     7141 tgaggtggtg gtggtagccg aggtatagac cttaagggtg cttcccgcat agatggtgtt
     7201 gttcctgaga ttgttccatg aacggaggtt ggctgcgcta accttgtatt tttcagctat
     7261 gccgcccagt gtttctcctt tctggacctt gtggctgatc agatttcccc ttgtccggga
     7321 aggctcggca gaaggtgttg ccgcagcagc tgtttcagta cggggagctc ctgtcctgct
     7381 tgtataaatg ataagccgct ggttggcaac tatcctgtct gaacggagac cgttccaata
     7441 cttgaggtcg gcaatccgta ccccgtaacg gtaggctata tgactcaatg tctcaccgga
     7501 tagtaccctg tgagttatct gcgtatcgga agcagtggcc cttgtgttca tgatcagggg
     7561 attgaaatag acactgtcct tgaatgaata aatggaatcc tgcaaggccg caaagtcccc
     7621 ggtgtgttca aagggaagac gcagtatata ggggcgttcc actccgggga tgatatcctt
     7681 gacatattgc ggattcaggt cccggagctg gtcttttgaa atattgagat agttggttat
     7741 ctgttcaaaa tgaagcatcc tgttcacctc gaatgtatcg acatgcgcag gcatctctac
     7801 gggaagcgga cggatctggt gttccggata atagcgcaag gtataaagcg ctgcaacaaa
     7861 agccggcaca tatccgcgcg tttcacgagg gagaaaagga tagatatccc agaattcccg
     7921 ggatcctcct gccctgcgga tggccttgtt cacattgccc acaccgcagt tgtaggatgc
     7981 tatagccagg atccagtcac cgtatatacg gtaggaatca agcaaatacc tggcagcggc
     8041 atctcccgag acaacaggat ccagcctttc atccacatat gagttgatgg tcaggttgta
     8101 attccgggcc gtacggtaca tgaactgcca catcccggtg gctcttgccc tggataccgc
     8161 tctgggattg agggccgatt cgattaccgc catcgctttg agttcaaggg gcatcccgta
     8221 ggaatcgaat atctcttcga acaggggcat gtaatattga gccagcccca gtatggtgga
     8281 aatgcggtcc ggcattttct gggtataatg gatgatgtgg tttctgacaa tattgttgta
     8341 gggcagtttg atgaaagaat tcatcctggt gagcctgtcg atatagacag aatcggggat
     8401 agccgaagtc agcagcgcgg tatccagggt ttccagtgta tgtgaatcgg gcatctggtt
     8461 caactggttc tgatgataat acaagctcag tcgctgctct gtggaaaggg aatcgtaatc
     8521 aaaatccgga tacgtttctg tttcatcaaa tcctccttca ttgatcccat tgtaataaaa
     8581 cgatgtgtca gtttccggca attctgcctg caaaagcatt ctttgcaacg agtctgtaag
     8641 cctgaggagg gaatctacct gttgtgtcaa ttgtttcttt gtcggccttc tcatgatctg
     8701 tgcagaagaa gatgtaacaa accatgtcaa ggagaggatt agcaggatta aaaatttgga
     8761 tttcataagg ggtactttgt aattaaaagg ttaatctcat ttgtatgccg gcacccgggt
     8821 aacctgcaga agcgtacgtg gccaccggaa tcagacccgg ttcgacacgc agggtcaggt
     8881 catcgctgat gtcaaaatcg cggaaatgcg caaaaacatt ggcatctatc acattcaggg
     8941 catatacaag cacagtggct atgatggaat aatcacggta ccggcggaat gtgtcacggt
     9001 accatttgag gttttcaggg gtcatccaac cgtcgtattc agatggcttt gccatggcat
     9061 ggttgtacat ggtcttgtaa cgattgtact gcagtccgtt gtattgccag aaataaccgc
     9121 aggcaataag gcctccgtat atgacgggta ttttccagta gtccccgttg taaacctgtc
     9181 caagtcccgg aagcagtgcg gaatacaagg tggcttttgc cggcaggaag gccttttcat
     9241 gcttaaaacc tgccacaccg tccagcaggc ttccccagta aaacaatccg gctcccacat
     9301 agcaaagatt ctggtataaa gcataggaat cgttttcagt ttccttgtat ttgttatgga
     9361 aacgcgttcc cataaaaatt ccggcaccca taccggcata aacaacgggg atcttccagt
     9421 actgccggtt gtacgcctgg gaatagccag gcaggactac agctccggaa aatacgcggc
     9481 ccagtttcag ggtgtccttc ccggccaaag ccgaaaaata ggtacgtagt ttgaagggct
     9541 cttttgcctg aacggtagta tcttgtgatg acttagtgtc cttttgcccc atgtcaacgg
     9601 caaactgccc atagacaagg gaacctgaac acaataaaca caaaatgaat atcaggctac
     9661 gtttcacagg taaagaaccg gctaaatatt gtttttctca agagcttcca gaaatgcttc
     9721 catttcagaa tcgctggaac agtaaaccgt caaaacgcct tcacccctgg catttctttt
     9781 caggctaatg ttattgttga aatacgttcc gatccgctcc agtaccctgt aatagacatc
     9841 aggcaattcg gcttcccgtg cctgtgtttc attcttttct ggtcctgcag ggcgcaccat
     9901 ggcctccacc tgccggacag agagtccctt ttctacgatc ttttttgcta gtgccagccg
     9961 ggcgtcctgt tcttccaggg agagcagggc acgggcatgt cccatggtca gggcacctgc
    10021 ccgtataaac aactggatct gggccggcag ctggatcaac cgcaggtaat tggcaacagt
    10081 ggctcttttc tttccgacac gctcggccaa ttgttcctgt gtaaggtcgc attcttctgt
    10141 caaacgctga aaaccgtatg cgatttccat agcatccagg tcttcacgct ggatattctc
    10201 taccagtgcc atttccagca aggcgttgtc ttccacctgc ctgatatagg caggtacttt
    10261 ggtcaggccg gccatcctgg ctgccctgag cctcctttca ccgctcacaa gctgatactt
    10321 accgacggct agttttcgaa gagtcaatgg ttggatcagc cccaagttcc ttatggatgc
    10381 tgccagctcg gcaagagcct caccgttgaa ttccttcctt ggctgttccg ggttaacttc
    10441 aataagatca atatcgatca ctgaagttcc ttccacctgg ggaaggacca cgggatttct
    10501 gggcttccag tcactcaaag agggtgtttc tgatattagt gcacccagtc ctttgcccaa
    10561 agcgggtttc ttagtcatgc tggtttttct ttaaaaattc cctggccagg ttcaggtagt
    10621 tgttactacc tacggaagcc gcatcgtaaa ggatcaccgg tttcccatgc gagggagcct
    10681 cgctcaggcg tacgttccgc tgtatgacag tctgaaatac catttcctgg aagtggtgcc
    10741 ttatctcttc cacgatctga ttgtgtgaac gcaggcgaac atcgtacatg gtcagcagga
    10801 aaccttctat ggcaagggca gggttcaacc ggttctgaac aattttaatg gtattgagca
    10861 acttgcccaa tccttccagt gaaaaaaact cacattggac aggtatgatc actgaatctg
    10921 ctgcagtcag ggcattgact gtgattaggc ccagggaggg cgaacaatca atcaggatgt
    10981 aatcaaaatg atggcttact gtgtccaggt gggatttcaa taccccttcc ctttcgggta
    11041 caggcaacaa ctctatttca gcgcccacaa ggtctatgtg tgaaggaatg agtttgagtc
    11101 cctgtatctc ggtctggatg atcgttttct caatgggcat tttgccaatg agacattcat
    11161 aaagggtgtc accgctgctt gcattgggat cgtaacaaag ccccgatgtt gtgtttgcct
    11221 gtggatctgc atctaccaac aacacgtttt tttccatcac agccaatgat gctgcaaggt
    11281 tgatggccgt tgttgttttt cctactcctc ccttctgatt tgctatcgca attacttttc
    11341 ccataagatc cactgttaga tttcaatttg gcaaatatac attaattacc ataaatattc
    11401 ttaattttga cttctcaatt catagtcata tatcatgtcc ttaacacaat ggttgatcat
    11461 tcttaatcct gtggccggag gaggaaaaac agcccggatg tggccccata tttcaaaata
    11521 cttcctggaa cgggatttca tttttgaatg tgttttcaca aaacacaaga atcatgccgt
    11581 tacaatagtc attgaagcta tccgtgaagg tttccggaag tttattgtga tcgggggaga
    11641 tggcacctta catgaagtga tcaacgggat ttttttccag caggatgttc ccaccagcga
    11701 tattaccata ggtgtcatac ccataggaac aggcaacgat acggtttcgc cctacgggat
    11761 ccctttagaa tacaggcaag ccatggatat tatatgcaat aataaaagtg tcctgacaga
    11821 cgttggaacg gtgactttcc aggattacgg ggttgaacgc aagcgttttt ttattaatag
    11881 tgcaggtctg ggactggatg ccgaagtggt ccggcactac gaaaaagtcg tggagcagca
    11941 tcatgacagg ggaaaatccc tttacctgca aagcttgttg aacactttca tgttctaccg
    12001 gagcaaagac tacaggatcc ttttggacgg agaagtgttt tatgaaggac cggtgctcac
    12061 cttaaatatt ggaataggga ccacgatagg gagcggtatg aaagcccttc cccttgctat
    12121 ccccgatgac ggattgtttg acatcaccct ggtcacgtcc atgagtaagc tgtcgctggc
    12181 gctgaaaatc aaggatttca ttaatggaaa ggtctatagt ttcaaagagg caatgcatgg
    12241 caggggaagg acggtggaag tgttgcccaa cagtaaacag gcaacgggcc tggaaacgga
    12301 cggtgaactg atgggatcgc ctccctatgt attcaatatc attccttcat caatacgtat
    12361 ctttgcagga gaaagttttg ttccgtttgt tagaaacgaa acctgatttg cactttagat
    12421 tcgcacacgt atgattcctt gtacattgcc gatactttaa accagatgtc tgtggcagga
    12481 aagggtgagt acgtcagatt caggcaggca cggatgcctt ttccataaag tgcagggact
    12541 gaatacccgt acaaaacgtc cggttcataa atatatattc ttgattccca ggactctgta
    12601 tggaacaggg ccaggcggaa cgataaccgg aatgtttcct tcacgttttc atattccaat
    12661 tgggcataag cagtgcctcc ccattcgctg ctgttttcaa ggagcaggca atacatcccg
    12721 tctccacgca atgccagctg aaaggcatcc gaaaacctga ctgctgtacg caggcatagc
    12781 gattgcctgt ccagggtacc tttatccgat agggacctct gctgacggaa caataacatg
    12841 tggtgatccc tgtgcacctg caggcggtaa tcccatccat aggaaggatc atgacacagg
    12901 taccgggccc ggggaaagaa gtaggcccac gcgctcagat ccatcttcca tgcgcagggc
    12961 aaaacaagtg taccggtcat ttgcaggctg tactcattgg aagggtcagc attccttccc
    13021 agccgggcac tgtaggctgc cgtataggaa ggcgtgaaat accttagctg cataccggcc
    13081 tggttcccgt tttttccgta ataaatgata cctgccagcg ccgcaggaga acccccaagg
    13141 tcaagagccg cctcggaaaa aaaacgccat gaaggcaatc tgaatgacag gtctgcagca
    13201 ataccaccga atggtacagt acggttttta taatcattgt aataggttat cctgccggca
    13261 tcggggtgat tgtagccata tccgagtgcg gtcacaccgg ctttccatct ttcagctgac
    13321 caggatagat taaggccggt cacccatgac cggagcgtat tttttttctc atgttccaaa
    13381 ggggtgcggt ggtacccggt ttccacaaga gatgtgaagc cttcctccac aatgcgtgca
    13441 tccagagtcc tataagatat gaaaacagac aggtccaggc tcctgcgacg gaaagaggct
    13501 cccagtcccc tgaaaaaaag attttcatcc cttgaccggt atggtgtaat acccatttcc
    13561 tgtttgcaca acgaagcggt agaggaagct tttccaaact gtgttccatt ccatagcagg
    13621 agtccctggc cgaatcttgc acgaaaatcg cctatgacca gggttctgaa aggtttgaca
    13681 tctcttatct ctgcatgaaa agagagaaaa tccactacct gcttttcacc cggatcactt
    13741 tccagtgtga agccccacct gaccctgtcc ctgtaacggt aatcataccg caggtacctg
    13801 taccagggta ctccctggta acgtgagtta ggtcgtgcct ggtattcagc ttcagttatg
    13861 ggactgtact cctctccccg ggggaaaaaa gtccgtgtct gaacaaggaa tgagcgtttt
    13921 ccatgcctga acatggattt cagttgcaac ctgtcatatg tcgcctccgg ttccaggtga
    13981 aagaacaggg aaagcaggcg ggtgtcctgc tctgtaaagc cgggaatcag aaaaacttcg
    14041 gcccatgaaa tgatggcacc gtagcgttgc ctgtaatcga taagagaagc cacctggtaa
    14101 ggtgacaaaa gccctatcag gcggagatct tcaccggtgg ccgtgttcag actgattcgc
    14161 tcatcaagca tctcctcaag gtcctgtccg gtgaattcat attcccgggc caggttctct
    14221 aacatgacat ccctgtcctg tgcctgtgca tgacagatca ttgcggtgca taagagcacc
    14281 cataaaacat ttctcatgat cattccctcc tgttgcgctc ccctctgatg agcctggttt
    14341 tattttaata gttcagattt tgaaacggta tttgatttct ttgatggatt cgttggcttc
    14401 ttcaattttc aggttcagat tgtgctgaaa atcaatcatt cgttcggcaa ggttgctgtc
    14461 ggacagggcc aggatgcgag ccgcaaatat ggcagcattc cgggcaccgt caagggccat
    14521 ggtagcaact gggatcccgg cgggcatctg caatatggac agtatggaat ccagcccttc
    14581 cagggatttc gccgatttca ccggaacccc gatgaccggg atggacgtat atgcggcaat
    14641 gatgcctggg aggtgcgctg ccccgcctgc gcccgctatg atgaccttta tccccctttg
    14701 tgatgcttcc tgtgcatatt cggcaacctt gtccggggtg cggtgtgccg aaagggcctt
    14761 gatttcaaaa ggtatctcca tctggttcag aaagcgcgct gcttcttcca ttacagacat
    14821 atccgatatg ctgcccatga ttatgcttac ttgcggattc attatcagac tctattaaaa
    14881 gttcgaatat aatgattatt tttgttctat ggaacgcata accctacaag ataaatcttt
    14941 ccggacatac atcccgtacc aggaaatcga taaggctatt gaacaggtgg ccgccgcttt
    15001 gaatcgtgac cttcgaaacg aagacgagcc ggtctttatc gggatcctga acgggtcatt
    15061 catgtttacc gcagatctga tgaaaaagat cgacctcagc tgtgtggtta cctttgtcaa
    15121 gctgtcatcc tacagcggca cctccagcac aggaacggta agagagatcg tgggtttatg
    15181 cacagatatc accgaccgca ctgtggtcgt cgtcgaagat gtggttgaca gtggccaaac
    15241 cattgaaaaa ctggtggaag atttgaaaaa aaagaatccc cggaaaataa taatatgtac
    15301 cctgctattc aaacccaatg cataccgggg aaaaatcccc attgattatg tatgcatcag
    15361 gatccccaac gattttgtgg taggttacgg tctggactac aacaatctgg gaagacaact
    15421 caaagacctt tacgtaatag attagataat gctcaatatt attattgcag gccctccggg
    15481 atcgggcaag ggaaccctgg ccaaattgat gatagaagaa ttcggatttg ttcatctttc
    15541 tacaggagat atgctccggc acgaaatcgg cctgggctcg gccctgggaa aggaagtgga
    15601 agccatactg gccagggggg acctggtctc ggatcagata gtgatttcgc tgatcgcgaa
    15661 caatctgaaa gccaatccgg gggcacgcgg ttttctgttt gacggttttc cccgtaccct
    15721 gccgcaggca gaggcgctgg atgattttct ggaaaagtca ggacacccgc tgcagctgat
    15781 gatcctgctg aaagtggacg accaggtttg cgagatgcgc ctgctgcgcc gggcaggcat
    15841 agaaaaccgg cctgacgaca tggatccgga aagggtgcgt caccgtttgg ccacctatta
    15901 tgcccagaca gtacccatca tagaccatta taaaaaacag ggaaaattcg ttggcgtaga
    15961 tagttcaaaa acgcccgaat atacactcaa ccaggttagt gaatgtctga aacaaatttt
    16021 gtagattata taaaattgtt ttgtgcctct ggaaagggag gcgcaggttc agtacatctg
    16081 ctgcgggcaa aatatgttcc aaagggcgga cccgacggag gagacggagg ccggggaggt
    16141 catataatcc ttaaggtaaa tccgcaattc tggacattga tccacctcaa atacagaaaa
    16201 cacatccggg cagaacatgg tgaggccggc aggggcaatc tggcccacgg agccgatgga
    16261 aaggacgtca tactggaggt cccggcgggg accgttgtaa aaaacgcgga ggacgagcaa
    16321 atacttgccg agctgacaca gccggggcaa gaattcatcc tctgcaaagg gggccgtggt
    16381 ggtctgggca acacccattt caagtcgcct acacaccaga caccgcgttt tgcccagccc
    16441 ggagaagcag gccaggaagg ctggttcatc ctggaattga aacttctggc cgatgtaggc
    16501 cttgtgggtt tcccaaactc cgggaaatca acccttttgt cagccgtgtc ggctgccagg
    16561 ccaaaaattg cggattatcc gttcaccact ctcgagccgc aactgggtat tgtatccctg
    16621 cccgatcacc ggtcatttgt gatggccgat atccctggga tcattgaggg agcgcaccag
    16681 ggccggggac tgggattgcg tttcctcagg catattgaac gcaattccat gctgctgttc
    16741 atggtaccgg cagactgccc cgatgtgacg gctcaatacc gtatattgct caaagaactc
    16801 ggaaagtaca aacccgagct gctggataaa cagcgcatgc tggcagtaac caaggcagac
    16861 cttctggacc ccggaggtga aaaaatgctc cgccgccgcc tgccccgctc tttaccggta
    16921 ttgctgatct catcaataac aggatataac atttcccagc tcaaggaaca gatctggaac
    16981 atcttatgga ctcaataatt gaactggacc ataagctttt ctttatcctg aacggggcac
    17041 acagtgactt cctggaccct gtaatgaaaa cattgtccaa cattcctgta tggatacccc
    17101 tgtacctgct catagcgggg ggctttttcc tggttttgcc ctggcggcag gccctggtcg
    17161 caatactggg gattttattc actttcctgc tcacagacca gctttcatcc acgctgataa
    17221 aagaatgggt gcatcgtttg cgtccctctc atataccgga atgggaagga actttccggc
    17281 tgcttgagga caagggagga ctatattcct ttgtatcgtc acatgccagc aatgtatttg
    17341 gcctggcctg tttcaccagt cttgtgtatc gtaagaaatg gtatacctgt ggcatttttg
    17401 tctgggctgc tttggttgcc tacagcagga tctatgtagg aaagcatttc cccctggatg
    17461 ttgtctgcgg agcgcttatc ggcctgctcc tgggatgggc cgtatacagg ctatacagac
    17521 ggattggccg gaaaattcaa acgaaatgcg ctgcataatc gatagtacaa cggatccgta
    17581 ctggaacctg gccgtagaag aatacctcct gaccgtgccg gaagaaccgg ttttccgcct
    17641 gtggaggaac gccccggcca tcatcgtggg acgcaaccag aacacaccgg accagatcaa
    17701 cagcgaatac gtccgggaac aaggtattgc cgtggtaagg aggctgagcg gcggcggagc
    17761 agtcttccat gacctgggaa acatcaattt cacatttgtg gatgcatatg ttcccaggac
    17821 cgaccccgga gaggcctttg cccggtttac gaggccaatc ctggatgttt tgcattcact
    17881 gggtgttgcg gcggaactgg aaggacgcaa cgatctggtg atcgagggcc gaaaattctc
    17941 cggaaatgcc atagcactgt ataaaaaccg tattctcatg cacggcaccc tgctcttcag
    18001 tgcttccatc cgtgacttgt ctcttgcgct gcaggtaaaa gcaagggaaa ggggccgggg
    18061 ggttgcgagc aatccgcgcc gggtgtgcaa tctctccgaa tacctgccaa agggcatgaa
    18121 aacggaagat ttcctggaag tgctgaaaag acacattgca ggcagcccgg acaagcagac
    18181 cagggaacag ccgcttgatc cacgggagtg ccagatcata gaaacattat gcagaaaaaa
    18241 atacagaact tatgcctgga attacggcag ggcaccgctc cggggagatc tccgcaggga
    18301 gcggttcgca gggggcaatg tagaaatact gctccaggta aataacggga tcatggaaga
    18361 agtgaagatc tatggcgatt acttttttct gtatccaaca gaggaaattg aacaggccct
    18421 tcagggatgt ccgcacaacc gggaagccat tgccggccgc ctggcacgat tctcactgga
    18481 tgactacttc cgcgacattc cccgggaagg cttgctggac ctatttatct gactgctgtt
    18541 ccagcaattc aagcattctg atggcacaca acgatacggg tgtcccgggt ccgaatacgg
    18601 caactgcccc ggcgtcgtag agttcctggt agtcctggcc cgggatgacc cctcccacaa
    18661 tgaccatgat atcttcccgc cccatctccc tgagggtttt cactacctgg ggcaccagcg
    18721 tcttatggcc cgcagccagc gaagataccc ccactatatg cacgtcgttt tctacagcct
    18781 gacgggcagc ttcttccggt gtctggaaca agggacccat gtcgacgtcg aacccgatat
    18841 cggcataacc cgtggctacc accttggctc cgcggtcgtg cccgtcctga ccaagcttgg
    18901 ctaccatgat acggggctgg cggccttccc gggcagcaaa actggccacc atttccctgg
    18961 cttttgcaaa gtaaggatca tccttcactt ccgataaata aactccctta ttcatacgaa
    19021 ttacagcttt ataacgtcct gccacttttt cacaggcatc cgagatctca cccagagtgg
    19081 ccctgttacg ggctgcttcc acggccagtt ccagcaggtt ccctgaatta tcagctgccg
    19141 ccctggtcag ggctgccagg gaagctgcca cctttttgga atctcttgtc tttttgagtt
    19201 cttccaggcg tgcgatctgt gaaaggcgga cctttgtgtt gtccacatcc agtatatcta
    19261 tgggatcttc tttttccagt cggtatttat taacacctac caccacctgt tccccggaat
    19321 ctatcctggc ctgggtacgc gcagcggctt cttctatcct gagtttggga ataccggttt
    19381 caatggcttt tgccatcccg cccaggtcct cgatctcctg tataagttcc caggctttgt
    19441 gagccatctg gtgtgtcagt gtctccacgt aatacgatcc ggcccagggg tcaatgcctc
    19501 tgcagatttc cgtctcgtcc tgcaggtaca attgagtatt acgggcgatc cttgccgaaa
    19561 aatccgtggg aagcgctatg gcttcgtcca gggcgttggt gtgcaggctc tgggtatgac
    19621 ccagggcagc agccatggct tccgtgcagg ttcgtgctac gttgttgaat ggatcctgtt
    19681 ctgtaaggga ccaacctgaa gtctggcaat gtgtccgcag tgacagggat ttcggatttt
    19741 tgggattgaa ggatttcacg atttttgccc agatcatgcg agcagcgcgc attttggcta
    19801 tttcctcaaa atggttcatg ccaatggccc agaagaatga gattctgggt gcaaaatcat
    19861 caatatccag acctgccttc accccggtcc tgaggtattc aagcccgtct gccagggtat
    19921 aggccatttc aatctcactg gtcgcgccgg ctt
//
LOCUS       DAOSOF010000017        19439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2810_length_19439_cov_5.019810, whole genome shotgun sequence.
ACCESSION   DAOSOF010000017 DAOSOF010000000
VERSION     DAOSOF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_19439_cov_5.019810"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLM68_01885"
     CDS             <1..624
                     /locus_tag="PLM68_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT54155.1"
                     /translation="HIDPSKVITVHNAVDFSTVEDREITRGVPEKIVTFLGRITFQKG
                     PEYFIEAAYKVLQKTKDVRFVMAGSGDLFNRAIRRVAKLGISPHFHFTGFLRGEDVKN
                     MFAYSDVYVMPSVSEPFGISPLEAMRANVPTIISKQSGVAEVLRHAIKVDFWDVNAMA
                     DAIYGLLTYPALSAMTVRCGLEEVNALKWDNAALKVKEIYESVTEEK"
     gene            640..1827
                     /locus_tag="PLM68_01890"
     CDS             640..1827
                     /locus_tag="PLM68_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOT54156.1"
                     /translation="MKKTLCLYFQVHQPYRLRQYRFFDIGKNHDYFDEFTNKTIMGRV
                     ASRCYIPANRIILDLIKEFDGAFRVAYSISGIALEQMEQFAPAALESFQELARTGHVE
                     FLAETYSHSLSSLISPRKFKDQIKVHVKAVEQYFGQKPKTFRNTELIYSDTIGEMVGG
                     MGFTTMLTEGARHIMGWKSPDYLYVNALNPKLKLLLRNFRLSDDIAFRFSNKSWADWP
                     LTAEKYAGWIAGALEKDDIVNIFMDYESFGEHQPASSGIFDFLKAMPGMILHHTNLEI
                     LTPSQAADKHQPIAPLNVPYAISWADEERDISAWLGNELQSEAFKNLYKIEDQVRQSN
                     DPDLQRDFRRLQASDHFYYMCTKFFSDGDVHKYFNPYDTPYEAFINYMNVLSDITLRA
                     TKK"
     gene            1830..6041
                     /gene="glgP"
                     /locus_tag="PLM68_01895"
     CDS             1830..6041
                     /gene="glgP"
                     /locus_tag="PLM68_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOT54157.1"
                     /translation="MNSEMNLNPDYLFEVSWEVCNKVGGIHTVIATKALHMSREMENR
                     HILIGPDVWRDTNQNPEFLEDPRLLRSWRDRAVQEGLRIRVGRWNVPGNPIAILVDFS
                     SHISKKDEIFTQIWKEFKVDSISGQWDYVEPALFGYASGIVVESFVRFNLAPHHKSVA
                     QFHEWMTGTGLLYLKSAKIPIATTFTTHATVLGRSLAGNMMPLYDNLEKYNSVEKARE
                     FNVVAKHSLEKTAAYYADAFTTVSDITARECSHFLEREVDIVTPNGFENSITPSAQTF
                     EQKMKEGRQTLLQTASLMLHRKVDDNAFLVGISGRYEFKNKGIDIFLESLAWLNRERG
                     PEDRQILAFIMVPANHNGPRKEYDNPFTSHYLNDPEYDPVMKVIREQGLTNLKENNVS
                     VFFIPCYLNGDDGLINKTYYELLIGLDLTVFPSYYEPWGYTPLESLAFHVPTVTTSLT
                     GFGHWVNTNYKEPHPGIEIVFRNDNNHASVVNDVAAVIRRMSMLSAEEALRMKENARL
                     VSTIALWENQLKYYKKAYEIALEKLIGNLGKFQTIKEESTICRPDQLRSVPTWSPVTI
                     QKQLPQRLNFLEVLSRNLWWSWNQKARKLFSGIDHNLWRDVEKNPIVLLEKIPYKKYK
                     ALERSKTFLEQLDKIEASFNQYMKMREKLTGPRVAYFSMEYGLDWTLKIYSGGLGILA
                     GDYLKESSDKGVPITGVGLLYRYGYFRQVLSAQGDQVSEREMVDFQKIPAVPVRDSQD
                     NWTTISIAFPGRNIYARIWRVDVGRTPLYLLDTDFEDNLAEDRSITHQLYGGDWENRL
                     KQELLLGVGGIRALRALGIQADVYHCNEGHAAFTGMERLREYVVNDNLSFEQAREVVR
                     ASSLFTTHTPVPAGHDAFTEEMLRPYFSHYPERLKTSWETLMALGKTDVCNPNEKFSM
                     SFLAANLSQEINGVSKLHGQISREILNPLWPAYLPQESHVDYVTNGVHYHTWTAPEWK
                     EVHARVFGPDFGTHHYNKECFTGILNVPDAEIWNVRNALRKKLTDRLVLTLTQTRNAP
                     EFYSPREVVAIRAALNKDILTIGFARRFATYKRAHLLFTNLDRLDALVNNPRHPVQFL
                     FSGKAHPHDKAGQDLIRRIIEISRMPRFLGKILFLPNYNMELAKRLIQGVDVWMNTPT
                     RPLEASGTSGEKAAMNGVMHFSVLDGWWVEGYRKGAGWALPEERTYESQEYQDDLDAA
                     TIYNMLEHEIVPMFYKRNQEGIPVEWVQHIKNTIAFVAGNFTTNRMMEDYEEKFYRPL
                     AKRHTDMVDGNYALAREMAYWKRKMEREWNSLEVISYTHPNDSKDILVLGSECESRIK
                     LYLGALSKDDIRVEMVITTQNNDGKVVIDEIVPYRLEKASNGEATYVCKLVPQTAGSY
                     YIASRIFAYNSNMPHRQDFPLVKWL"
     gene            6032..6910
                     /gene="ribF"
                     /locus_tag="PLM68_01900"
     CDS             6032..6910
                     /gene="ribF"
                     /locus_tag="PLM68_01900"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOT54158.1"
                     /translation="MVIAATGFFDGVHLGHRAVLTSLADTARKMGGKSLALTFWPHPR
                     AVLQQDAQKFRLLTSLDEKKKMMYDLGVDEIQIIPFTREFSMVSSRDFFREYLDRKYN
                     VKVLVAGYNHRLGCNGAYDPQLMDREAEMAGLTIIRVPEYVSDAGEHISSTKIRAALT
                     AGSMETANRWLGYPYSLHGVVVLGNRMGRTLGFPTANMQLYEPLKQLPADGVYKVEAE
                     VNGRWYRGMMNIGVRPTIANNLERIIETHIFDFEDNIYGLDIRVRILKFHRQEIAFPS
                     LDSLKEQLNKDKEALL"
     gene            6907..7272
                     /locus_tag="PLM68_01905"
     CDS             6907..7272
                     /locus_tag="PLM68_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54159.1"
                     /translation="MKSDTLITILLSILAIVFGIINARSKKRRTESRIPRHIDEELYT
                     LPEERPQFRFSVPETPAENIMEEAIRTTSGNEDVGLPVRPAEQTVKTSRQGERKFGFN
                     AKKAVIFSEILKPKFDEFG"
     gene            7361..7834
                     /gene="greA"
                     /locus_tag="PLM68_01910"
     CDS             7361..7834
                     /gene="greA"
                     /locus_tag="PLM68_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT54160.1"
                     /translation="MTKIHYITAEGLEKLKEELNVLTTVERVAAARAIAEARDKGDLT
                     ENSEYDAAKDAQAMLEMKIAKLQYMIANARIVDESRLNTNVVQIMNKVKIQNLKNKSI
                     MEVTLVGESEANLEQRKLAISSPIGKGLLGKKKGEIAQISTPSGIIEFEILEIAL"
     gene            7836..8231
                     /locus_tag="PLM68_01915"
     CDS             7836..8231
                     /locus_tag="PLM68_01915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOT54161.1"
                     /translation="MATIFSRIISGEIPSYKVAEDDRFYAFLDINPRKPGHTLVVPKQ
                     ETDYIFDLDPVMLGDMFRFAQRVARAIESAIPCKKVGVGVLGLEVPHAHIHLIPMDSE
                     GDMNLSRPACKIPEQEMQRICRMIAQQFE"
     gene            8239..9387
                     /locus_tag="PLM68_01920"
     CDS             8239..9387
                     /locus_tag="PLM68_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT54162.1"
                     /translation="MKIHRLYLVAALLGTTLFFSCTGSNTVRISGTVHQITEKEPLLF
                     KRLDFTSETLLDTISADEKGNFSYDLDKGVDQPGYYYLYAGERRIASLILKKGDRVHV
                     EVGTDGSPVSIEGSEESLLLQQVNDRLFQTVRQFDSLYVLYEKAATGEKDDLSLKLGS
                     LFIRYKQDAIRFLYQHPRAFVNTSLVFHEFPGPLYVFADTKDAPLLRRVYDSLYLDYP
                     LSPYVMAIRDRFESMEKALRMEQAFDRADVIGFPDICLPGIDGAPVCLSSLKGKTIIL
                     SFWHSANVEMRLDNRELMEIYDTYSPKGLEVFQVAIDTDKTAWALAVREQSLPWVSVC
                     DGLGGYSPALVTYNVGELPAYFIINSDEEILARTASLDEVARIVKSLL"
     gene            complement(9371..10066)
                     /gene="tsaB"
                     /locus_tag="PLM68_01925"
     CDS             complement(9371..10066)
                     /gene="tsaB"
                     /locus_tag="PLM68_01925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT54163.1"
                     /translation="MIETSTQVCSVSLTAGSRIAASRQTDMPKAHASLLAVFIRDILQ
                     ECGLSVRDCDAVAVSGGPGSYTGLRVGVSTAKGLCFGASLPLIQVNSLELIAQSYLDH
                     NTSVNPRRIYPMIDARRMDVYTAPYSAGTIDDKGRVAIMEGPVEALTIIAGRFAEDLE
                     QGLVVFTGNGASKCREVIQHPNAVIAPLESHASGMARAAMKAFREKKFEDVANYTPFY
                     LKEFQAIRPKADF"
     gene            complement(10059..11351)
                     /locus_tag="PLM68_01930"
     CDS             complement(10059..11351)
                     /locus_tag="PLM68_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT54164.1"
                     /translation="MKKSVLWIIISAVVLIVILIIVGKNRSGKGIEVTVANPLVKNIT
                     EVIPANGKIQPVTEVKISPDVSGEIVVLNVAEGDRVKAGQVLIQIKQDQYRSARDRMI
                     AALNQSRAQLAQQEAQFKQVELSYKRNLSLHEQGAISQAEFEASSSEYSIAGEQLNGA
                     RFNVQSAEAGLAEAEESLAKTTIFSPMNGIVSKLSVERGERVVGTSQMAGTEMLRIAN
                     FEEMEVLVDVNENDIVRINQLDTAIVEVDAYRGRKFTGVVTQIANSAKNIGSSLEQVT
                     NFEVRVFILPESYSDLLITESSTPFRPGMSASVSIQTETKYQAIAIPIQCITTRPELL
                     SDSLREQLGPNELVEQVFVLREDNTVQTVQVRSGLQDHIHIEILEGLTPQDKVVTGPY
                     SAIATTLENGSKVEPIEEKAEGKETKVTQVRATTHTVD"
     gene            complement(11365..12747)
                     /locus_tag="PLM68_01935"
     CDS             complement(11365..12747)
                     /locus_tag="PLM68_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT54165.1"
                     /translation="MKKSLGLILFLVFMLPLSLQAQKKWSLEDCIRYAWENNLTIKQQ
                     ELGVEQSENIHFQSKMDFTPSLSARVSENVNWGQSVDLSTLTILNHVRSSNTGVGIYA
                     DMDLFTGFQKLNTVKQERSKVNVAIQEVQKLRNDISIEITRSYLNVLLTGEILDAAVL
                     SRESVAEQCSRTRKLVDAGSQPLSALLEVESQLASEELQVVDAQNNLRTYLLTLKQLL
                     DLPMEESFEIITPAIGDPGEMEPVSIEDLYYKSLDLPQIKKTEHTKEQRQYELAIAQG
                     GRYPRLSLSFGYSSFYSDANRKKDDDTDETSSGTVGDLFGGSSASFWEQMKNNNNPSI
                     GLSLTIPIFSKWQVNTNVKNARLGLDMAELEIKNARQVLLKEVQQAANDATSTYLRTQ
                     AAQRNVAAMEESFRYMQQKFDIGMLNGTDYIVSKTNLFKAQSSYIQAKYEHVFKLKIL
                     DFYKGIPITL"
     gene            complement(12756..13475)
                     /locus_tag="PLM68_01940"
     CDS             complement(12756..13475)
                     /locus_tag="PLM68_01940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT54166.1"
                     /translation="MRKYLFIAYPVGLYLIFGLPVLLLSAILYSGGIDLLTANSVGAM
                     GITLLHMRRKRRKMTTLPAMPWKRMFLLTGIMMIPVVSFTAVLTGKAFLQIPLTPWLI
                     NCTWSFFWVACCEELLFREFLITRMQEARIPAWVILILPAVMFSLCHRPESLSLLLQR
                     FVLGTCFGFLYLKKRDIALCMATHWVYNMILYTFQYSLPDVTALYSASRAALNVLLCM
                     LSLLGIVVAYSIKKSKFVGYS"
     gene            complement(13532..13735)
                     /locus_tag="PLM68_01945"
     CDS             complement(13532..13735)
                     /locus_tag="PLM68_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54167.1"
                     /translation="MRSADYLQGFESLGDKACCAVIAGVSSPGVDEELAYKLGEILGQ
                     AFRALYDLGQNLIGKLKKSGVPA"
     gene            complement(13732..13956)
                     /locus_tag="PLM68_01950"
     CDS             complement(13732..13956)
                     /locus_tag="PLM68_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54168.1"
                     /translation="MSDFEMRDFGFVTLEASVQCVITGGDSEYGMFGELLQDLLNGLA
                     SKMGKAGFLVALLAKNIDSFIDGFRDGWKK"
     gene            complement(14029..16281)
                     /locus_tag="PLM68_01955"
     CDS             complement(14029..16281)
                     /locus_tag="PLM68_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOT54169.1"
                     /translation="MKKIRVRQHHISDCGAACLASVSAWFGLRFPLEQIRQWSGTQTH
                     GISVQGIKEGAQALGLEAMAYRRCSGSGAVDHNLLNSLPVPAVFHMHKENGHSHFVVF
                     YGPCRTITGKKRKYRIMDPEDGRMHRRRPEEIMQQWTGVVVTLSAGENFRKGNHIPGF
                     LYRTGVLMKGEKRMFLAALVASLLYTCTGIISALFLQQIADRIIPQGAGDFLRLLGFS
                     MVYIALCAMALNMFRVHFLLKTGFRTASRLVNGFYRHILYLPQRFFDQRQSGELVARI
                     NDAFKVSTFLSTGLLNLALSVFTLIISFVVMFLFNPGLSWICIGALPLYAAIYYLFDL
                     RNKDTQRRIMIAQATLETGLVDSLRSVAQIKYSTAQEQMTGKIQDRFGTYVRHAWKGG
                     MNQLWAATCGDTITRGMGLAILWTGAVFVTRDLMSFGELLTFYALTAYFSSPVSEIIS
                     FSQKYRDARIATERLFEIMELETEEDPVRHKAGAEGGKAGMVKAHAACPGHGKGSGCR
                     GGDLAVKDLSFHYPGRMMLFENFNCRFPSGSITGIAGESGSGKSTLGSLLMRMYNPDG
                     GTIFLDGTEAGSIPLDTWRRKLGIVPQKADMFEGNILENITLGDTSPDLERVASLCSE
                     LGLEPFLSSLPRGILTPLGEQGIQLSGGQRQRIALARAAYRDPSWIILDEATSSLDST
                     SELYVLNALRRWSHQGTGIIIIGHRMSTLSVASHILLLHEGKIAEEGSHAVLIQKKGK
                     YAAWCRQQGI"
     gene            complement(16244..16561)
                     /locus_tag="PLM68_01960"
     CDS             complement(16244..16561)
                     /locus_tag="PLM68_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54170.1"
                     /translation="MKTICRPSYPVLSRDTAGDHVLRDKGSIGFGVFFIAIVLATVSG
                     MIVFLSFVATELSVVREPVSAEKGNMRALFPGYSRREKERTAPFIMEEGYEEDTCQAT
                     PYK"
     gene            complement(16641..17144)
                     /locus_tag="PLM68_01965"
     CDS             complement(16641..17144)
                     /locus_tag="PLM68_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT54171.1"
                     /translation="MLEKFSFNYIDIILLLVWLIAITEGLMKGLIKQVVGILALIVAA
                     YCSFHFSGFAADWIVDWFNWNGEGLRIVAFVVTFIVVLIVVLLLGHLLDKLLKIVLLG
                     WLNRLTGAIFGWIKWNILLVILVYVLSLVDAYIPFLPKEAFESSRLFQVIEKFSSILL
                     PYLPFLD"
     gene            17202..>19439
                     /gene="ileS"
                     /locus_tag="PLM68_01970"
     CDS             17202..>19439
                     /gene="ileS"
                     /locus_tag="PLM68_01970"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT54172.1"
                     /translation="MKFPEYKNLDLPAIQEEITGKWDQEQTFRRSVEDRPEAPAFVFF
                     EGPPSANGMPGIHHVISRAIKDVVCRYKTQRGFKVERRAGWDTHGLPVELNVEKMLGI
                     TKEDIGVTLTVEEYNAVCRKEVMKYTREWELLTRKMAYWVDMSRPYVTYHNSYIETVW
                     YLLSEIYRKGLLYKGYSIQPYSPAAGTGLSTHELNQPGCYKDVKDTTCTALFRITKDN
                     NSDFLFEREDEPLYFLSWTTTPWTLPSNTALCVGPAIQYIKLRTFNPYSGGPISVIMA
                     RDLRETLFPEKNGEIPMDSYKPGDKATPWIHTGPVFTGKQLEGIRYSQLIPWVNPGEG
                     AFRVITGDFVTTQEGTGIVHIAPTFGDDDHKVAKKHGVPALILKDSLGNLCPMVDKTG
                     KFYRLEDLDAHFVETRIDSGSYAPFAGRYVKNAYDPHKDGQSGEAAGADSLDVEICMW
                     LKKNNGVFRIEKMVHNYPHCWRTDKPVLYYPLDSWFIRTTAVRERMMELNDTIRWKPE
                     STGTGRFGKWLENLQDWNLSRSRYWGTPLPIWRTEDGKEERCIGSLQELYHALDDSVA
                     RGFMKSNPLRDKGFVPGDYSKENYERIDLHRPFVDQWVLSSASGKSMRREPDLIDVWF
                     DSGSMPFSQEGLEKLGTPAFGQVADFIAEGVDQTRGWFFTLHAIATMVNDRVCFRNVL
                     SNGLILDKNGNKMSKRLGNTVDPFKMLDMYGADPLRWYMLTNAQPWDNLRFDETGVDE
                     VRRKFF"
BASE COUNT         5108 a         4909 c         4803 g         4619 t
ORIGIN      
        1 catattgacc cttcaaaagt catcactgtt cacaatgcgg tggatttcag caccgtcgag
       61 gaccgtgaga taaccagggg ggttccggaa aaaatcgtca ctttcctggg caggatcacc
      121 ttccagaaag gtcctgaata tttcatagag gctgcctaca aagttttaca gaaaacaaaa
      181 gacgtgaggt ttgtaatggc aggcagcggc gatttgttca accgcgccat aaggcgtgtg
      241 gcaaaactgg ggatttcccc tcatttccac tttaccggct ttttgcgcgg agaagatgtg
      301 aagaatatgt ttgcctattc cgatgtatat gtcatgccct cggtttctga accctttggc
      361 atatctcccc ttgaagccat gagagcgaac gtgcccacta tcatttcaaa gcaatccggg
      421 gtggccgaag tattgcgcca tgccatcaag gtggatttct gggatgtcaa tgccatggca
      481 gacgccatat acgggttgct gacctatccg gccttatctg ccatgaccgt acggtgcggc
      541 ctggaagagg tcaatgcgtt aaaatgggat aatgccgctc tgaaggtcaa agagatctac
      601 gaatcggtaa cagaagagaa ataacaaaaa accaatcata tgaaaaagac actctgtttg
      661 tactttcagg ttcatcagcc ttacaggtta cggcaatatc gtttttttga cataggcaaa
      721 aatcatgatt acttcgacga attcaccaac aaaaccataa tggggcgggt tgcatcaagg
      781 tgttacattc ctgccaaccg gatcatactg gatttgatca aggaattcga cggagccttc
      841 agggtcgcct attccataag cgggatcgcc ctggaacaaa tggagcagtt tgcccccgcg
      901 gcacttgaat ccttccagga gctcgcccgt acagggcatg tggaatttct tgccgaaaca
      961 tactcccatt ccctgtcctc acttatctct ccgagaaaat tcaaagacca gataaaggta
     1021 cacgtgaagg cggtcgaaca atacttcggc caaaaaccaa agacattccg caacacggaa
     1081 ttgatttatt ccgatacaat tggtgaaatg gtgggtggta tgggatttac caccatgctc
     1141 actgaagggg cgcggcacat catgggatgg aagagtcccg attacctgta cgtgaatgcc
     1201 ctgaatccca agctgaaatt attgctcagg aacttcaggc taagcgacga tattgctttt
     1261 cgtttttcca acaaaagctg ggcggactgg cccctgacgg cagaaaagta tgcgggctgg
     1321 attgcaggag ctcttgaaaa ggacgatatc gtcaatattt ttatggatta tgaaagcttt
     1381 ggagaacacc agccggcttc atccgggatc tttgattttc tcaaagctat gcccggaatg
     1441 atactccacc acacaaacct tgagatcctg accccgtcac aggcagcaga caagcatcag
     1501 cccatagccc ctcttaatgt accctacgcc atatcatggg ccgatgaaga acgggatatc
     1561 agtgcatggc tgggcaatga acttcaatcc gaggccttta agaatctcta taaaattgaa
     1621 gaccaggtaa ggcaaagcaa cgatcctgac ctccagcgtg attttcgccg cctgcaggcg
     1681 agcgaccatt tttattatat gtgcaccaag tttttttcgg atggggacgt gcacaaatat
     1741 ttcaaccctt atgacacccc gtacgaagct tttatcaatt atatgaacgt attgagcgac
     1801 attacgttac gtgcaactaa aaaatgatta tgaacagtga aatgaatttg aatcccgatt
     1861 atttgtttga ggtaagctgg gaggtttgca acaaagtagg agggatccat accgttattg
     1921 ccaccaaagc actgcatatg tcccgtgaga tggagaacag gcatattctg ataggtcctg
     1981 atgtgtggag agataccaat cagaatcccg aattcctgga agatccccgt ctgcttcgtt
     2041 cctggaggga cagagccgtg caggaagggt tgagaatcag ggtaggacgt tggaatgtgc
     2101 ccgggaatcc catagctata ctggtggatt tttcatcgca tatctcaaaa aaagacgaaa
     2161 tatttaccca aatctggaaa gaatttaaag tcgactccat cagcggccag tgggattatg
     2221 tagaaccggc actgttcgga tacgcctcgg gaattgtagt tgaaagtttt gttcgcttta
     2281 acctggcgcc tcatcataaa agtgtagcac aattccatga atggatgacc ggaacagggc
     2341 ttttgtacct gaaatcggcc aagatcccca ttgccacgac atttacaact catgccactg
     2401 ttctgggacg ctcccttgca gggaacatga tgcccctgta tgataacctc gaaaagtaca
     2461 attcggttga aaaggcacgg gaattcaatg ttgttgcaaa acattccctg gaaaagacgg
     2521 ctgcatacta cgccgatgcc tttacaacgg taagcgacat taccgcccgg gaatgcagcc
     2581 attttctgga aagggaagta gacatcgtga ctcccaacgg gtttgaaaac agcattaccc
     2641 cctcggccca aacatttgag cagaagatga aagaaggcag gcagaccttg ttgcaaacgg
     2701 cttccctgat gctgcaccgt aaagtggatg acaatgcttt cctggtgggc atcagtggcc
     2761 gctatgaatt caagaacaag ggcattgaca tcttcctgga atcactcgca tggctgaaca
     2821 gggaaagagg acccgaagac aggcagatac tggcctttat catggtgccg gccaatcata
     2881 acggtcccag aaaagaatat gataatccct ttacctccca ttacctgaat gatcctgaat
     2941 acgacccggt tatgaaagta atacgtgaac aggggctgac caacctgaag gagaacaatg
     3001 tgagtgtttt tttcattcca tgttatctga acggagatga cggactgatc aataaaacgt
     3061 attacgaact gctcataggc ctggatctca ccgtctttcc ttcttactac gaaccctggg
     3121 gctatacccc gctggaaagc ctcgcttttc atgtgcccac tgtaaccaca tccctgaccg
     3181 gcttcggcca ctgggtcaac acaaactaca aggaaccaca tcccgggata gagatcgtat
     3241 tccgcaacga caacaaccac gcctctgtgg tgaatgatgt ggccgcggtg atccgccgta
     3301 tgagcatgct gtcagcagaa gaagcgttgc gcatgaagga gaatgcccgc ctggtatcaa
     3361 caattgcctt gtgggagaac caattgaagt actacaaaaa agcctatgag atcgccctcg
     3421 aaaagctgat tgggaacctg ggaaaattcc agacgatcaa ggaagaatcc actatatgcc
     3481 gccccgacca gctgaggtcc gtaccaacct ggtctcctgt gaccatccag aaacagctgc
     3541 cgcaacgtct gaacttcctg gaagtattat ccaggaacct ctggtggtca tggaatcaga
     3601 aggcaaggaa acttttttca ggaatagacc acaacctgtg gagggatgtg gaaaaaaacc
     3661 ctatcgtcct gctggagaag atcccttaca agaaatacaa ggccctggaa agaagcaaga
     3721 cattcctgga acaactggac aaaatagagg catcgttcaa ccaatacatg aaaatgcgcg
     3781 aaaagctgac cggtcccagg gtagcctatt tcagcatgga atacggtctg gactggacgc
     3841 tgaagatata ttccggaggc ctgggaatcc ttgccgggga ctacctgaaa gaatcatccg
     3901 acaagggagt tcccataacg ggagtgggac tgttgtaccg ctacgggtat ttccgccagg
     3961 tcctgtccgc acagggagac caggtgagtg agagggaaat ggtcgatttt cagaaaatcc
     4021 ctgccgttcc cgtacgtgac agccaagaca actggaccac catcagcata gctttcccgg
     4081 gacgcaacat ctatgcccgc atctggaggg tggatgtggg acggaccccg ctctatctgc
     4141 tggataccga ttttgaggac aacctggccg aggacaggag catcacccac cagctatacg
     4201 gcggtgactg ggaaaacagg ctgaaacagg aattgttgct gggtgtgggc ggaatacgtg
     4261 ccctccgtgc cctgggcatt caggccgatg tttaccactg caacgaagga catgctgcct
     4321 ttaccggaat ggagcggctc agggaatatg ttgtgaacga taacctttcc tttgaacagg
     4381 ccagggaggt ggtgcgcgca tcatccctgt ttaccaccca taccccggta ccggcaggtc
     4441 atgatgcctt taccgaggaa atgctccgtc cctatttttc gcattacccc gaaaggctta
     4501 aaacctcatg ggaaacactc atggccctgg gcaaaacgga tgtctgcaat cccaatgaaa
     4561 aattctccat gagctttctg gctgccaatc tttcgcagga gatcaatgga gtaagcaaat
     4621 tgcacggaca gataagccgg gagatactca atccgctatg gcctgcatat ttaccccagg
     4681 aatcgcatgt ggattatgtg accaacgggg tgcattacca tacctggacg gctccggaat
     4741 ggaaagaagt ccatgcacgc gttttcgggc cggattttgg gacgcatcat tacaacaaag
     4801 aatgtttcac gggaatcctc aacgtgccgg acgccgaaat ctggaatgtc cgcaatgctt
     4861 tgcggaaaaa actgacggac aggctggtcc tgacccttac ccagacacgg aatgctccgg
     4921 aattttattc acccagggaa gtggttgcca tcagggcagc cttgaacaag gacattctga
     4981 ccattggttt tgcacgacgc tttgctacct ataaaagagc tcacctgctt ttcaccaacc
     5041 tggacaggct ggatgccctt gtcaacaatc ccaggcatcc tgttcagttt ttgttttcag
     5101 gcaaagcaca tcctcacgac aaagccggcc aggacctgat aaggcggatc attgagattt
     5161 cacgtatgcc ccgttttctg ggcaagatcc tgttccttcc caattacaac atggaattgg
     5221 caaagcgcct gatccagggg gtggatgtct ggatgaatac gcccacacgt cccctggagg
     5281 catcggggac cagtggagaa aaggcggcca tgaacggagt gatgcatttc agcgtactgg
     5341 acggatggtg ggtggaaggg taccgaaagg gtgccggatg ggcattgccc gaagaaagga
     5401 cctacgagag ccaggagtac caggacgacc tggatgccgc aaccatatac aacatgctgg
     5461 aacacgagat tgttcccatg ttctacaaga gaaaccagga agggatccct gttgaatggg
     5521 tgcagcatat caagaatacc atagcctttg tagcagggaa tttcaccacc aatcgcatga
     5581 tggaggatta cgaagagaaa ttctacagac cgctggccaa acgtcatacg gacatggttg
     5641 acggcaatta tgccctggca agggaaatgg cttactggaa aaggaagatg gaaagagaat
     5701 ggaattccct ggaagtgatt tcctatacac atcccaacga cagcaaggat atcctggtac
     5761 tgggaagtga atgtgaatca cggataaaac tatacctggg cgctctttcc aaggatgata
     5821 tcagggtgga aatggtgatt accacccaaa ataatgacgg gaaggttgtc atagacgaaa
     5881 ttgttccgta ccgccttgaa aaagcttcca atggtgaggc cacttatgtg tgcaaactgg
     5941 ttccacagac agccggatcg tattacattg cctccaggat cttcgcgtat aattcaaaca
     6001 tgcctcacag gcaggatttc ccactcgtaa aatggttata gcagctaccg gattttttga
     6061 tggtgtccac cttggccacc gcgccgtact gacatccctg gcggatacag cacgaaagat
     6121 gggtggaaag agcctggcgc ttaccttctg gccccatccc agggctgtct tgcaacagga
     6181 tgcacaaaaa ttccgcctgc tcacatccct tgatgaaaag aagaaaatga tgtacgacct
     6241 gggtgtcgac gagatacaga tcataccatt taccagggaa ttttccatgg tctcctcacg
     6301 ggatttcttc agggaatacc ttgaccggaa atacaatgtc aaagtccttg tggcaggcta
     6361 caaccacagg ctgggctgta atggggcata cgatcctcag ctcatggacc gggaagcaga
     6421 gatggccggc cttacgatca tacgtgtacc ggaatatgtg tccgatgcag gtgaacatat
     6481 cagttccacc aagattcgtg cggccctgac tgccggttcc atggaaacgg ccaaccggtg
     6541 gcttggttat ccctacagcc tccacggggt ggtggtattg ggaaaccgga tgggaaggac
     6601 actgggtttt cctacggcca acatgcaatt atacgaaccg cttaaacagt tgcctgcaga
     6661 cggtgtttac aaggttgagg cagaggtcaa cggaagatgg taccgaggca tgatgaatat
     6721 aggagtccgt cctacgatag ccaataattt ggaacgtatc attgaaacgc atatctttga
     6781 ttttgaagat aatatttacg gactcgatat ccgggtccga atactgaaat tccacaggca
     6841 ggaaattgcc tttccttccc tggattcgtt aaaagaacag ttgaataagg ataaggaagc
     6901 cttattatga aatcagatac actaatcacc atactgttga gcatcctggc catcgtattc
     6961 gggatcatca acgcacgctc caagaaaagg agaacagagt cccgcatccc caggcatatc
     7021 gacgaggaat tgtacaccct tcctgaagaa aggccccaat tccggttttc cgttcctgag
     7081 acccccgcgg aaaatatcat ggaagaggca atcaggacga cctcaggcaa tgaagatgtc
     7141 gggttgccgg tcagacccgc ggagcaaaca gtcaaaacct cccggcaagg tgaacgtaaa
     7201 tttggcttca acgctaagaa ggccgttata ttttccgaga tcctgaaacc aaaattcgac
     7261 gaatttggtt gattcataaa gaagtattat atttgtgctg agaagagtcc tgccagcgca
     7321 ggattcttct tattctttat ttattcttta agattgtgat atgaccaaaa ttcattatat
     7381 aacagccgag ggtctggaga aattaaaaga ggaactgaac gtgctgacca cggtggaacg
     7441 ggtagcggcg gcacgtgcca ttgccgaggc ccgggataag ggtgacctga ctgaaaactc
     7501 cgagtatgat gctgctaaag atgctcaagc catgctggaa atgaagatag ccaagctgca
     7561 atacatgata gccaatgcca ggatagtgga tgaatcccgt ctcaacacca atgtggtaca
     7621 gatcatgaac aaggtcaaaa tacagaacct gaagaataaa tccatcatgg aagtcaccct
     7681 ggtgggggaa agtgaggcaa acctggagca aaggaaactg gctatatcgt ctcccattgg
     7741 aaaggggctg ctggggaaga agaaaggaga aatagcccag ataagcactc cctcgggtat
     7801 tattgagttt gaaatactgg aaatagcgct ttgatatggc tacaatattc tcacgcatca
     7861 tttcaggtga aatcccttcc tacaaggtag cagaggacga ccgtttctac gcctttcttg
     7921 atataaatcc ccggaagccc ggccacacac tggttgtccc taaacaggaa accgactata
     7981 tattcgatct ggaccctgtc atgttgggtg atatgttccg ctttgcccaa agggtggcaa
     8041 gggccattga atctgctatc ccgtgcaaaa aggtgggtgt cggggttttg gggctggaag
     8101 tcccccatgc gcatattcat ctcatcccta tggactcgga aggagatatg aaccttagcc
     8161 ggcctgcatg caagatcccg gaacaggaga tgcaacgtat ctgccggatg atagcccaac
     8221 aattcgaata atcccatcat gaaaatccac agactttatc ttgtggcagc cctactgggt
     8281 accaccttat ttttctcatg caccggcagc aacaccgtac gcatcagcgg cacggtacat
     8341 cagattaccg aaaaagaacc gctgctgttc aaacggctgg actttacatc ggaaaccctg
     8401 cttgacacga tttccgccga tgaaaagggc aacttcagct atgacctgga caaaggagtg
     8461 gatcaacccg gttattatta cctgtatgcg ggggaacgca ggattgcctc gctcatactc
     8521 aagaagggag accgggtgca tgttgaggtc gggacagatg gcagtcctgt cagcatagag
     8581 ggatcggaag aatcgctgct gctgcagcag gtcaatgacc gtctctttca aacggtaagg
     8641 cagtttgatt ccctgtatgt gttgtatgaa aaagcagcca ccggggaaaa agacgatctg
     8701 agcctcaagc tgggttcact ttttattcgc tacaagcagg atgcgatccg tttcctatac
     8761 cagcatcccc gtgcctttgt caatacttca ctggtattcc atgaatttcc cggcccgctg
     8821 tatgtctttg ccgacacaaa agatgctcct ttgttgcgca gggtatacga ttcgctatat
     8881 ctggactatc ctctttcccc ctatgtgatg gccatcaggg acaggtttga aagcatggaa
     8941 aaggccctgc gaatggagca ggcttttgac cgtgccgatg taataggttt tcctgacatc
     9001 tgtcttcccg gtattgacgg agccccggtg tgcctttcat cccttaaggg gaaaaccata
     9061 atcctctctt tctggcatag cgccaacgtc gagatgcggc tggacaaccg tgaattgatg
     9121 gaaatatatg atacctattc gccgaaaggg cttgaagtct tccaggtggc gatagatacc
     9181 gacaagacag cctgggcttt ggccgtaagg gaacagtccc tgccctgggt cagcgtgtgt
     9241 gacggactgg gggggtattc tcctgccctg gtaacctata atgtggggga actcccggct
     9301 tatttcatca ttaacagcga tgaggaaatc cttgccagga cagccagtct ggatgaggtt
     9361 gccaggatag tcaaaagtct gctttaggac ggatagcctg aaactctttc agatagaagg
     9421 gcgtataatt ggccacatct tcaaatttct tctcccggaa ggccttcatg gcagccctgg
     9481 ccataccgct ggcatgggat tccaacgggg cgattactgc gttgggatgt tgtatcacct
     9541 ccctgcattt tgaggcaccg ttcccggtaa agacaacaag cccctgttca aggtcttctg
     9601 caaaccgtcc cgctattatg gttagggcct caaccgggcc ttccatgatc gccacgcggc
     9661 ccttgtcatc tatggttccg gcagaataag gagcagtata aacatccatc ctccgggcat
     9721 ctatcatggg gtagatgcgc cggggattta cagaagtgtt gtgatcaagg tatgattggg
     9781 cgatcagctc cagtgaattc acctgtatca ggggcaggga agctccaaag cacaagcctt
     9841 tggctgtgga tactcccacc cgtaatcccg tgtatgagcc tggaccgcca cttactgcaa
     9901 cggcatcaca atcccgcacg ctcaggccgc attcctgcag gatatcgcga atgaagacag
     9961 caagaagtga tgcatgggct tttggcatgt ccgtctggcg actggcagcg atccggctac
    10021 ctgcggtcag cgagacagag caaacctgcg tgcttgtttc aatcaacagt atgtgtggtt
    10081 gcacgtacct gagtgacttt tgtttccttt ccttcagcct tttcttcaat gggttctacc
    10141 ttggacccgt tttcaagcgt tgttgcaatg gctgaatagg gacctgtcac caccttgtcc
    10201 tgtggtgtca gcccttcaag aatttctata tggatatggt cctgcaatcc ggatctgacc
    10261 tgcaccgttt gcacggtatt gtcttcgcgc aatacaaaaa cctgttctac cagttcattg
    10321 ggcccgagtt gctccctcag actgtctgac agtaattcgg gacgtgttgt gatgcattgg
    10381 atgggaatgg cgatggcctg gtattttgtt tcggtctgga tggaaaccga ggccgacatg
    10441 cccggcctga agggagtgga cgattccgta atgagcaggt cgctgtaaga ttccgggagg
    10501 ataaagaccc tgacttcaaa attggtaacc tgttcaaggg aagaaccgat attcttggcc
    10561 gagttggcta tttgcgtgac cacacccgtg aacttgcggc cgcggtaggc atccacttcc
    10621 actatggccg tatcaagctg attgatccgg acaatgtcat tttcattcac atccaccaac
    10681 acctccattt cctcaaaatt ggcaatgcgc agcatctcgg tcccagccat ctggcttgtt
    10741 cccacaacac gctccccgcg ctccacgctc agcttggaaa caataccgtt catgggggaa
    10801 aagatggtgg ttttggcaag gctttcctct gcttcggcaa ggcctgcctc ggcactctgg
    10861 acattgaagc gggctccatt caattgttca ccggctatgg aatattcaga ggaagaagct
    10921 tcaaattcgg cttgtgatat ggcgccctgc tcatgcagtg acaagttgcg cttgtaggaa
    10981 agctccactt gtttgaattg ggcctcctgt tgcgcaagtt gtgccctgga ctggttcaat
    11041 gcggctatca tcctgtcacg ggcagacctg tactggtcct gcttgatctg tatcaacacc
    11101 tgtccggctt tcacgcgatc gccctcggca acgttcaata caacaatttc acccgataca
    11161 tcggggctga tcttaacctc ggttaccggc tggatctttc cgttggcggg aatgacctcg
    11221 gtaatattct tgacaagagg atttgcaacg gttacttcta tgcccttacc ggaacggttc
    11281 ttgcccacga tgatcaggat gacaataagc acgaccgcac ttatgatgat ccacaatact
    11341 gattttttca tgtaaatgat aattttataa cgtaatggga atgcctttgt aaaaatccag
    11401 gatcttcagt ttgaacacat gttcgtattt tgcctgtata tagctgctct gggctttgaa
    11461 caaattggtt ttggaaacta tatagtctgt accattgagc atcccgatat caaatttctg
    11521 ttgcatgtaa cggaacgatt cctccatggc tgccacattc cgttgtgcag cctgtgtcct
    11581 gaggtaggta gaggtggcat cgttggcggc ctgctgcact tccttaagca gaacctgccg
    11641 ggcattcttt atttccagtt ctgccatatc caggcccagc ctggcatttt tcacattggt
    11701 attgacctgc cacttgctaa aaatggggat ggtaagcgaa aggcctatgg agggattgtt
    11761 gttgtttttc atttgttccc agaagctggc cgatgatccg ccaaaaagat cccccaccgt
    11821 tccggagctg gtttcatcag tgtcgtcatc ttttttacgg tttgcgtccg aataaaacga
    11881 ggaataccca aatgacaggg aaagcctggg atagcggcca ccctgggcaa tggccagttc
    11941 gtactgccgt tgctcctttg tatgctctgt cttttttatc tgcggcaggt ccagtgactt
    12001 gtaataaaga tcttcaatgg aaacaggctc catttcaccg gggtctccta tggcaggggt
    12061 aatgatctcg aaagattcct ccatgggaag gtcaagcaat tgtttcagcg tcagcagata
    12121 agtacgcagg ttgttttggg cgtctaccac ctggagctct tctgatgcca gctgggattc
    12181 aacctccagc agggcactca ggggctgact tcctgcatca accagtttgc gtgtacggga
    12241 gcattgttca gccacgcttt cccggcttag aacggcagcg tccaggattt caccggtcag
    12301 cagcacgttc aggtaactgc gggttatctc tatggaaata tcattccgga gtttttgcac
    12361 ttcctgtatg gccacattga ccttggaccg ctcctgcttt accgtgttga gtttctgaaa
    12421 acctgtgaaa agatccatat cggcatagat ccccactcct gtattgctgc ttctgacgtg
    12481 gttcagtatg gtcagtgttg acaggtcaac cgactgaccc cagttgacgt tttcactgac
    12541 gcgggcacta agggaagggg taaaatccat ttttgattga aaatgaatat tttccgattg
    12601 ttccaccccc agttcctgct gtttgatggt taggttgttt tcccaggcat aacgaatgca
    12661 atcctccagc gaccattttt tttgagcctg tagggaaagg ggaagcatga aaaccaggaa
    12721 cagaatgaga cctaaacttt ttttcatagt gctagttaag aatatccgac aaatttactt
    12781 ttttttatag aatatgcaac aacgatgcct aaaagcgaaa gcatgcacaa caacacattg
    12841 agcgcagcgc gtgatgctga atataacgct gttacatcag gcagcgaata ctggaaagtg
    12901 tagaggatca tgttgtagac ccaatgtgtt gccatacaaa gggcgatatc cctttttttg
    12961 agatacagga agccgaagca ggtcccaagg acaaagcgct gcaacaacaa tgaaagggat
    13021 tccggtctgt gacacaggga aaacataacc gccggaagta taagtatcac ccaggcaggt
    13081 atcctggcct cctgcatcct ggtgatgagg aactccctga acagcaattc ctcgcagcat
    13141 gcaacccaga aaaacgacca tgtgcagttg atgagccagg gtgtcagtgg aatttgtaaa
    13201 aaagcctttc ccgtaagaac cgcggtaaac gacactacag gaatcatcat gatccccgtc
    13261 aacaggaaca ttcttttcca tggcatcgca ggcaaagttg tcattttcct tcgcttgcgc
    13321 cgcatgtgca acagggttat tcccatggcc ccgacggaat tggccgtcaa cagatcaatt
    13381 cccccggaat agagtatagc ggataacagc aggacaggta agccaaagat aagatatagc
    13441 cctacgggat aggctatgaa taaatacttt cgcatctttt ttagatcaag aaaagagacc
    13501 agccaaactt agttggccag tctcattttc atcatgccgg aacgcctgat tttttcaatt
    13561 ttccaatcag attttgtccc agatcgtaga gagcacggaa tgcctgtccc aggatttcac
    13621 ccagtttgta tgccagttcc tcatccactc ccggtgagga cacgcctgcg atgactgcgc
    13681 agcaggcttt gtcgcccaac gactcaaagc cctgtaaata atcagcactt ctcatttctt
    13741 ccagccgtct ctgaatccgt caatgaaact gtcaatgttt ttggccagca atgccacaag
    13801 gaatcctgcc ttgcccattt tcgaggccaa accgttgagg aggtcctgga gtaattctcc
    13861 aaacataccg tactcggaat ctcctcctgt tattacacac tggactgaag cctccagggt
    13921 aacaaaccca aaatcccgca tctcaaaatc actcataatt ttcaggtttt ttaaatattc
    13981 tctcaccgtt caggcgcgtg agattccgcc tgcaatcgcg cgtttgcctt atattccctg
    14041 ctgcctgcac caggcagcat attttccctt cttttgtatc aaaacagcat gtgatccttc
    14101 ttctgcgatt tttccttcat gcaacagcag gatgtgcgaa gcgacagaaa gggtggacat
    14161 cctgtgccct atgataatga taccggttcc ctggtggctc cacctgcgca aggcgttgag
    14221 cacatacaac tccgatgtgc tgtccagcga agaggtggcc tcgtccagga ttatccagga
    14281 ggggtccctg taagccgccc tggccagggc aatccgctgg cgctgccctc ctgatagctg
    14341 gatgccctgt tcccccaaag gtgtgaggat cccgcgcgga agggaagaga gaaacggttc
    14401 cagtcccagt tcactgcaaa gggaagccac ccgctccaga tcgggcgagg tatctcccag
    14461 ggtgatattc tccaggatat tgccttcgaa catatcggcc ttttgcggca cgatacccag
    14521 tttcctgcgc caggtatcca gggggatgct tccggcttcg gtaccgtcca ggaagatggt
    14581 gcccccgtcc gggttgtaca tgcgcatgag caaggagcca agggtacttt tacccgaccc
    14641 gctctctcct gcaataccgg tgatactgcc cgatggaaac ctgcagttga aattttcaaa
    14701 caacatcatc ctgcccgggt aatggaacga caggtctttc acggcaaggt ctccacccct
    14761 gcatccagaa cctttaccat gccctggaca agccgcatgg gccttgacca taccggcctt
    14821 cccaccttcc gcacccgcct tgtgccgcac cggatcttcc tcggtttcca gctccatgat
    14881 ctcgaacagg cgctcggtgg caatacgtgc atccctgtat ttctggctga aagaaatgat
    14941 ttcgcttacg ggggaagaaa aatatgcagt aagggcataa aacgtaagca attccccgaa
    15001 gctcataagg tcccgtgtca cgaaaaccgc ccctgtccac aggatggcca ggcccatacc
    15061 acgggttatg gtatccccgc aggtggcagc ccacaactga ttcattcctc ccttccaggc
    15121 atggcggaca tacgttccga accggtcctg tatttttcct gtcatttgtt cctgtgccgt
    15181 gctgtatttt atctgtgcaa cgctccgcag cgaatctacc agtcctgtct caagagttgc
    15241 ctgggctatc atgatcctgc gctgtgtgtc tttgttcctc aggtcgaaca agtagtatat
    15301 cgctgcgtac aacggcaacg caccaataca gatccagctc agtccgggat tgaataggaa
    15361 catgaccaca aacgaaataa tcagcgtgaa tacgctcagt gccaggttca gcaaaccggt
    15421 gctcaggaat gtgcttacct tgaaggcatc gttgatgcgt gccaccagtt ctccagactg
    15481 cctctggtca aagaaacgct ggggcaggta taatatatgg cggtagaaac cgttcaccag
    15541 tctggaggcc gtcctgaatc ctgttttcag caggaaatgg accctgaaca tgttcagggc
    15601 catggcgcac aatgcaatat acaccatgga aaatcccaac aagcgcaaaa aatcacccgc
    15661 accctgcggg atgatacggt ctgcaatctg ttgcaggaac aaagccgaga tgatccccgt
    15721 gcatgtatac agcagggaag ctacaagcgc ggccaggaac attcgttttt cccccttcat
    15781 cagtacgcct gtcctgtaaa ggaatcccgg gatgtgattg cctttcctga aattttcacc
    15841 ggcagacaat gtgaccacca cgccggtcca ctgttgcatg atctcttccg gtcttctgcg
    15901 gtgcatcctc ccgtcctcag gatccatgat gcggtatttt ctttttttcc cggtaatggt
    15961 tctgcatggt ccgtaaaaca ccacgaaatg gctgtgcccg ttctctttat gcatatgaaa
    16021 tacagcaggg acgggaaggc tgttcagcag attatggtcc actgcccctg aaccactgca
    16081 acggcggtat gccatcgcct ccaggcccag tgcctgtgcg ccctctttga tcccctgtac
    16141 cgaaatgccg tgagtctgtg ttccgctcca ttgccttatc tgctccagag gaaagcgcaa
    16201 tccgaaccag gcagagacag acgcaaggca ggccgccccg caatcactta tatggtgttg
    16261 cctgacacgt atcttcttca tatccctctt ccataataaa cggagctgtc ctttctttct
    16321 ctctcctgct gtaaccggga aacagagccc gcatattccc tttttccgcg ctcacaggtt
    16381 ctctcacaac agacagctcc gtagcaacaa acgataaaaa gacaatcatg cccgaaacag
    16441 tagccagcac tatcgcgatg aaaaatacgc caaaccctat gctcccctta tcgcgcaaga
    16501 catgatcacc agctgtgtcc cgggacaaaa caggatagga aggtctgcag attgtcttca
    16561 taccacaaac ctagacccta gacagggaaa ttaacagaca aataggataa atgattaaac
    16621 agctgccgcc agacgccggc tcaatccagg aaggggaggt agggaagcag tatggaggaa
    16681 aatttctcta tcacctgaaa cagccgtgat gattcaaaag cctctttcgg caggaatggg
    16741 atataggcat caacaaggct caatacgtaa accaggatta ccagcaggat attccatttg
    16801 atccatccga atattgcccc tgtcaacctg ttcagccatc caagcaggac aattttcagg
    16861 agcttgtcca gaagatgccc taaaagcagg acaacaatca gcacaacaat gaaagtaacc
    16921 acaaacgcca ctatgcgtaa cccttctccg ttccagttga accaatccac tatccagtcc
    16981 gctgcgaacc cgctgaaatg aaaggaacag taagcggcca cgatcagggc aagaattcca
    17041 accacctgtt tgatgagccc tttcatcaga ccctctgtta tggctatgag ccataccaat
    17101 aaaagtatga tatcaatgta gttaaaagaa aatttctcca acatgctatc aaagataggt
    17161 aagattcgta tctttgcaaa ctatttttat tgcctttttt tatgaagttc cctgaatata
    17221 agaatcttga cctgccagcc atccaggagg aaatcaccgg caaatgggac caggagcaaa
    17281 ccttccgccg gagcgtcgaa gaccgtcctg aagcacccgc attcgtgttt tttgaaggac
    17341 ctccttctgc aaatggaatg ccaggtatcc accacgtgat ttccagggct atcaaggatg
    17401 tggtctgccg gtacaagacc cagcgcggct ttaaagtgga aagacgtgcc ggctgggata
    17461 cacacggact tcccgtggag ctgaatgtcg agaaaatgct gggaatcacc aaggaagaca
    17521 ttggggttac cctgacagta gaagaataca acgcggtatg ccggaaagaa gtcatgaaat
    17581 atacccgcga atgggaactc cttacccgga agatggcata ctgggtggat atgagccggc
    17641 catacgtcac ctaccataac tcgtatatag aaactgtctg gtacctgcta agtgagatat
    17701 acaggaaagg cctgttatac aagggatatt caatacaacc ctattctcct gcagccggaa
    17761 cggggcttag cacccatgaa ctgaaccagc ccggttgcta caaagacgta aaggacacca
    17821 cgtgtaccgc cttgttcagg attacgaagg ataacaatag tgactttctt tttgaaaggg
    17881 aggatgaacc gttgtacttt ctttcctgga ccaccacccc ctggacactg ccctccaata
    17941 cggccctatg cgtaggaccg gctatccagt atatcaaact gcgcacattc aacccgtaca
    18001 gcgggggtcc catatcggtc atcatggccc gcgacctcag ggaaaccctc ttcccggaaa
    18061 aaaacgggga aattcccatg gactcctaca aacccggaga caaagccaca ccctggatac
    18121 atacaggtcc cgtctttaca ggaaagcaac tggaaggcat acgttacagc cagctcatcc
    18181 cctgggtgaa tcccggagag ggggctttca gggtgataac cggtgatttt gtaaccacac
    18241 aggaagggac aggtatagta cacatagctc caacctttgg agacgatgac cacaaggtgg
    18301 ccaaaaagca tggcgtacct gccctgatcc ttaaagacag ccttggcaac ctgtgcccca
    18361 tggtagacaa gacaggcaag ttttacaggt tggaagacct ggatgcccat tttgtggaaa
    18421 ccaggataga cagtggatcc tatgcacctt ttgcgggccg ttatgtgaaa aacgcatacg
    18481 atccccacaa ggatggccag tcaggagagg cagccggagc agactccctg gatgtggaga
    18541 tctgcatgtg gctcaagaaa aacaatgggg tgttccgtat tgaaaagatg gtgcacaatt
    18601 atccccattg ctggcgtacc gacaaaccgg tgctgtatta tccgcttgac tcctggttca
    18661 tccgcaccac agcggtcagg gaacgcatga tggagctgaa cgacactata agatggaaac
    18721 cggaatccac cgggacgggg cgttttggca aatggctgga gaatcttcag gactggaacc
    18781 tctcccggag ccggtattgg gggacaccgc tgcccatttg gcgcacggaa gacgggaagg
    18841 aagaacgctg catcggctct ttgcaggaat tgtaccatgc cctggatgac tcggttgccc
    18901 gtggattcat gaagtccaat cccctaaggg acaaaggttt tgttccgggg gattacagca
    18961 aggagaacta cgaaaggata gatttgcacc ggccttttgt ggatcagtgg gtcctttcct
    19021 cggcaagtgg aaagtccatg cggcgcgagc cagatttgat tgacgtgtgg tttgactcgg
    19081 gctcaatgcc cttttcgcag gaaggcctgg aaaagctggg gacccccgct ttcggccagg
    19141 ttgccgattt cattgccgag ggagtggacc agacccgggg ctggtttttc accctgcatg
    19201 ccattgccac catggtcaac gaccgcgtct gtttccggaa cgttctctcc aacggactca
    19261 tcctggataa gaacggcaac aaaatgagca aacgcctggg caatacagtg gatcccttca
    19321 aaatgctgga catgtacgga gccgatcctt tgcgctggta catgcttacc aatgcacaac
    19381 cctgggacaa cctgcgcttt gacgaaaccg gcgtggatga agtccggcgc aaattcttt
//
LOCUS       DAOSOF010000018        19341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2830_length_19341_cov_4.615472, whole genome shotgun sequence.
ACCESSION   DAOSOF010000018 DAOSOF010000000
VERSION     DAOSOF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_19341_cov_4.615472"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..780
                     /locus_tag="PLM68_01975"
     CDS             397..780
                     /locus_tag="PLM68_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54173.1"
                     /translation="MSKFIIVIIFSFLTASLYGQVTVTGHISAEIIEGVEVSSKMLSG
                     FEVEPFSEKKELGEIILSCDESVACNVILEFGKLIDKYGNGVILETSVEGDENKLRFL
                     ASTRNLRGKKTNLYEGSFSVMLFYE"
     gene            complement(784..1257)
                     /locus_tag="PLM68_01980"
     CDS             complement(784..1257)
                     /locus_tag="PLM68_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOT54174.1"
                     /translation="MKKLIIVALTCLLTISAFGQSRFYKKFSSYEGMTKVYISPSMFS
                     LMGDGMQLDLTEDVNIAGMIKNMTGLYVLSTSNKGHITEMKKDFSDLVKTGEFEVLLE
                     VEDGDEKVIIYLQKQEDIITDLYICADENNEFSVIYLAGKMTSEDLKEMMKGVKK"
     gene            complement(1262..1663)
                     /locus_tag="PLM68_01985"
     CDS             complement(1262..1663)
                     /locus_tag="PLM68_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54175.1"
                     /translation="METDRIKKIMDKQYQEIEVPVDLEARLSATIDRLAAEESQVVAE
                     RPVRRIPLWKALAVAASVAIIITLSVSLPLHDRPVYIADTFTDVEDAYQETEEVLQYV
                     SSLMNKGVNKVSETHKNISTINTVNKYIKIK"
     gene            complement(1650..2165)
                     /locus_tag="PLM68_01990"
     CDS             complement(1650..2165)
                     /locus_tag="PLM68_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54176.1"
                     /translation="MFDAQTFKDTFLPWHGHLYRTAYRLLNCQADAEDTVQDVFVKLW
                     NMRENLSGIKNLQAYATTMTRNLCLDRMRSSVYRNSDNSAAESLRYEWIDAVEQIDQQ
                     DELKMVLVLVDQLPQKQREIFRMRHFNELSLEEIRQLTGLSPVNIRVQLSRAQKKVKE
                     QYERINCHGNR"
     gene            complement(2158..2343)
                     /locus_tag="PLM68_01995"
     CDS             complement(2158..2343)
                     /locus_tag="PLM68_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54177.1"
                     /translation="MKTIETTREGLYPMVVIVVLTIALLVMAFTWKSNNQISTKFVSE
                     QATAESLQELVIGIKNV"
     gene            complement(2346..2810)
                     /locus_tag="PLM68_02000"
     CDS             complement(2346..2810)
                     /locus_tag="PLM68_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOT54178.1"
                     /translation="MKKIFITCLLLAGTVLVSYAQKDYEDLVQQFSCQPAAEHIHVGS
                     FAMRLAEIFADKDDAQFISGIRSVSILSLECCDINIKEAFRQQAQRLSEKGLELIAEV
                     SDESDQLRILAEVRNDKIHRFIVINVGEEPCLIQINGSIDLSCMEKMISQNI"
     gene            2907..4211
                     /gene="purD"
                     /locus_tag="PLM68_02005"
     CDS             2907..4211
                     /gene="purD"
                     /locus_tag="PLM68_02005"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT54179.1"
                     /translation="MKVLVLGSGGREHALAWKLAQSPLVSDLFVGPGNPGTSSLTLTN
                     GKQVQNVALVTNDFQSVENVLLQYAIDMLVVGPEEPLVMGITDFCKNREGLENLLVIG
                     PDSRGASLEGSKDHAKAFMTACRIPTALYRTFTCGQYAEACLYLDSLEPPYVLKADGL
                     AAGKGVLIEPDLVKARAGLKEMMEGKFGAAGNKVVVEQFLRGTEVSVFILTDGTHYKI
                     LPEAKDYKRIGERDQGPNTGGMGAVSPVPFADRSFMKKVEERIIRPTLEGLARKGCDY
                     RGFLFFGLINCNGDPYVIEYNVRMGDPETESVMMRLQGDLAAACYSLKTKTLDKMSLS
                     AAENHCVTVVAVSGGYPGTYQKGYAIKGLDQVKEANVFHMGTSTTPQIVTKGGRVLAV
                     SATGPTLLSARELAYRELSLIAYTDMVYRNDIGEDLMDMKYS"
     gene            4213..5160
                     /locus_tag="PLM68_02010"
     CDS             4213..5160
                     /locus_tag="PLM68_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54180.1"
                     /translation="MIQFIKRSVWGAVLVSLLIMTVFRVFYHFSQEVALPWYLHLLSA
                     AMILVTGMLLSYLNDRYDLLPERTQWITVAYVWFVSCCPQTYDNLWAQGAALLIAAAT
                     YKLVYSAYVPVTRSGPFMSAFFISCAGLLFFPAFFLYIPLLISFIRMAKVSGKDLLAF
                     AGGMLSPLFLTSALLWFMKHDVALFWGHRLENFKQIYPAKTLEAFSISGTVMVALIAL
                     LVMLSLFIRLVRRDTTTTVNVSRFYSSMFWFVLFSCLVMLTYTGFAYGYIPIMMIPVS
                     LLITSLFTGKRFFGSVLMISLLIIASLVYYVCRVFLFPY"
     gene            complement(5146..6534)
                     /locus_tag="PLM68_02015"
     CDS             complement(5146..6534)
                     /locus_tag="PLM68_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOT54181.1"
                     /translation="MKHFLSILWKITGTLIILIGLGFFIARVINVAPSNKLSVQEQVV
                     TIVEEGGCFVCHGGPDFYPNINVPVFGHMIEKKAEKGVRYANAFSVLMHLNNDEAVDE
                     SILIKMQKVISERSMPPLSYSIIHWKSAIIKDKQKILLHWLYDHLSTEHDWPVPTKER
                     TFEPIIPVPDSLPANLSKVNLGRILFNDPRLSADSTVSCATCHKLDQGGADNMNFSLG
                     AFAQPGRYSTTTIFNAAFQQFFFWDGRVSSLALQSALMLTDPVIMGNESFEEITDRLE
                     ADPDFTSLFTATYTEGFSAENITDALAEYHKVLITPDAPFDQFLKGDGEAISPDAKKG
                     YDLFKKIGCASCHNGVAIGGNSLEYMGLSNKFFPSRDKVQLPDLGRYRVTGLQEDIYK
                     FKVPGLRNVALTYPYFHNSSAPDLETAVKTMAFYQRDKTLTLKEIREVVSFLRTLTGT
                     FEGKLLTGSIGE"
     gene            complement(6536..7324)
                     /gene="ccsA"
                     /locus_tag="PLM68_02020"
     CDS             complement(6536..7324)
                     /gene="ccsA"
                     /locus_tag="PLM68_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT54182.1"
                     /translation="MTWEHHLIFAAVAAVFWIAGSLLAIAKFSKRYVWTIIAYTTGVV
                     IYAAYITGLWISLHRPPLVTMGETRLWYSLFLSLAGLITYIRWRYKWILSLTTVMSLV
                     FVAVNVLRPGIHDRVLMPVLQSIWFVPHVTVYMFSYALLACTFLMAIAGLLKPSNDVL
                     PAIDTLCKAGLSFFTIGMLLGALWAKQAWGDYWTWDPKETWAAITWLLYALYFHIKEI
                     RPNAKRMIFVSLILAFISLQICWYGLQYLPQARGSLHNYEIKVS"
     gene            complement(7321..7983)
                     /locus_tag="PLM68_02025"
     CDS             complement(7321..7983)
                     /locus_tag="PLM68_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOT54183.1"
                     /translation="MTINTALFAFPVNLIIGLSMVLAAWKFKCLSSDRHMTIALLLMI
                     AAALVQGFMPAQASFTRSWPFVIVLTWFLTVLASRLFRKFSWAGLGLWIALWSGMLGS
                     ADATVTRVVVPREEFTQTLLPFGMRMDEFQVDRYQTGEPMEYRAQIILRDANREQSKT
                     LRVNHPVHFRGHQLYLMDYDTSKGSESDYCIVMVTRQPWRWLVLAGILIMLGGAFKTF
                     IL"
     gene            8050..9192
                     /locus_tag="PLM68_02030"
     CDS             8050..9192
                     /locus_tag="PLM68_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOT54184.1"
                     /translation="MGKLNIVVGKSKDETLTEELKRSGLPVVLYGIGLIADTTRIFLQ
                     EKGIRVSCRVVDDKYLDPAAHHADSDHILSISQLQKEFSAYNLLLCFFGGYKNDLQPY
                     RQLFPGARMIDFLSSIYEGGALQSMESSYVLAHKPAFDSVYELLEDSLSQDSMEAYIN
                     AKINKDASYLFPFVRRPQYFSEIDQVPALKLHQNEVFVNCGAFTGDTIKDFLEVTSCH
                     FDKIYACEPDSANLQKLTRFIQSAGIAERTEIVPKCISDKAGTVNFLNQGSMLSHKTE
                     APGPGVVAMQADTIDNILNSRPASLIVMDVEGNELKALEGARKTILAHQPLLALAAYH
                     KKDDLPVLTSFLKNLLPAYRFYFRVHKPMAIDAVLYASTRNEPVPA"
     gene            complement(9221..9291)
                     /locus_tag="PLM68_02035"
     tRNA            complement(9221..9291)
                     /locus_tag="PLM68_02035"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9257..9259),aa:Cys,seq:gca)
     gene            complement(9313..9759)
                     /locus_tag="PLM68_02040"
     CDS             complement(9313..9759)
                     /locus_tag="PLM68_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT54185.1"
                     /translation="MYEEILVSADSLLRDESDHVANMANVSALIKEKFNFWWVGFYRV
                     DSKAKELILGPFQGPAACTRIPFGRGVCGTCWQQRKTIIVPDVEKFPGHIACSSRSRS
                     EIVVPVFDRERNVRAVLDIDSEKINTFDQMDRQYLEELTGLFKNNY"
     gene            complement(9811..10359)
                     /locus_tag="PLM68_02045"
                     /pseudo
     CDS             complement(9811..10359)
                     /locus_tag="PLM68_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(10340..11413)
                     /locus_tag="PLM68_02050"
     CDS             complement(10340..11413)
                     /locus_tag="PLM68_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54186.1"
                     /translation="MTRMTDLFRNMVLLLLMGTGLLAGSCGDGKQEVAPPLPVIQEML
                     EDTASAYYIDPLGYSQSMKMLPIGVIGNSADDLQVVETLLGLDEFDNITGKPGSDGIR
                     DFAGEHFEFLCLPSFETLSREDMIRQIVRLFRQQDQRPPVKALVVAGDLVPPEVYDDL
                     KDLIGMGNLGIITVSVIQAGNDKALEYVHKNNAGRDKRLTVGVIATDGAFSINAYDCL
                     ADVTQVVFQAYEDKETNSTEKAIRFAMTSLADKLPVNNQARMKYIVLGAHPLLQHREL
                     IPELLTFLRNYRKDDRYVYRDLLDSDMILIEPQKEAGIQLYRAMREERLAAFRTTPSC
                     VRVYEIEKPDLTKYPLLNVCINN"
     gene            complement(11397..12584)
                     /locus_tag="PLM68_02055"
     CDS             complement(11397..12584)
                     /locus_tag="PLM68_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT54187.1"
                     /translation="MNDLEAGKYSKRGVSSSKSEVHDALEGLSRGLYPKAFCKIIPDI
                     LTGDGEHCLLMHADGAGTKSSLAYCYWKKTGDLSVWEGIAQDAIVMNLDDLLCAGVTN
                     DLLLSSTIGRNKRLVPGEVIRAIIQGSRHFCDKMQLYGIRLELTGGETADVGDLVRTV
                     IVDSTVCARIRRDQVIDNARITAGDVVVGLSSFGQTTYEDAYNSGMGSNGLTGARHDV
                     FSKAVARMFPESYDTGIPDEMTYSGTMDLTDIEPETGMTYGKLVLSPTRTYAPVIKAI
                     LGKYRNQVHGMVHCTGGGQTKVLHFIDNLHVIKDNLFPVPALFSRIREIGGTPWKEMY
                     TVFNMGHRMELYVPPVVAPALCSMAAAFDLEARVIGRVEGWTGRRVSVESPAGTFTYD
                     KND"
     gene            complement(12602..14785)
                     /locus_tag="PLM68_02060"
     CDS             complement(12602..14785)
                     /locus_tag="PLM68_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOT54188.1"
                     /translation="MSTFRQKALLEFSSHKAERFCVENKPVSSFFGQHVFDLKKMKNY
                     LPEEAYEAVVKAIEKKCKIDRSVATHVAEGMRSWAMERGATHYTHWFHPLNETTAEKH
                     EAFIRPYANGCAIEQFVGDALIQQEPDASSFPSGGLRNTFEARGYTAWDPGSPAFILD
                     QTLCIPTIFVSYTGDALDVKTPHLKSLRALDQAATAVVRYFDPETESVNVIYGLEQEY
                     FVVDLALYRARPDLMLCDRTLQGHTAAKDQQLEDHYFGNIPSRVMAFMKDFETEAYKL
                     GVYIKTRHNEVAPNQFEFAPYFEEANLAVDHNQMIMLVMRKLAKKHNLKVIFHEKPYA
                     GINGSGKHCNWSLFTNNGINVLSPGKTPQDNLQFLSFIACTLKAVHQHHFLIMSAVAT
                     LSNSYRLGGTEAPPAVLSAFMGSTLADLLDVIDKGGPGRGRSSERTRMDVVSMVPEIF
                     PDNTDRNRTSPFAFTGDRFEFRAIGSSANAGSAMLFLNTAVAQQLKSFKVLVDKEIEK
                     GEKLEDALMKIIRKFIRESRDIRFEGNGYSDEWHKEAAERGLRGITDVPASYDALLEE
                     QSIALMEEMCVMTRRELEARYEVRNQMYVKKLQIEARVLGDLTINHFVPAAIRHENML
                     LENVKGLRDLFPPEEYQELVSTQLKAIRIIAGYVNEMRGLVHLLIEARKKANNVELLH
                     DRAREYAAAVLPVMDRIRDCADHLEMIVDDELWPLPKYRELLFLR"
     gene            14889..16085
                     /locus_tag="PLM68_02065"
     CDS             14889..16085
                     /locus_tag="PLM68_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT54189.1"
                     /translation="MLGISELSRKMPASPIRKLVPYAEAAKKRGIKVYHLNIGQPDID
                     SPKEALEPIRNIDLKLVSYPHSAGIESYRRGLAKYYQDVNINLTYEQIIVTIGGSEAL
                     QIGLKVCCDPGDEVIVFEPFYTNYNTFGLMAGVRFVPVTTTIENGFALPPMSEVEKYI
                     TPRTKAILINSPGNPTGTLYSKEDILQLGEIVKKHNLFLLSDEVYREFCYSDEPHFSC
                     MNIPGAEQNVILIDSVSKRYSLCGVRIGCIASHNKDVMSAVLRFAQARLCAPALGQIA
                     SEGALATPREYFEAVKQEYIDRRDLMIERLNKIPGVYSPMPMGAFYTTARLPIDDADK
                     FAQWLLESFNYKGQTVMVAPAAGFYATPGKGLDEVRIAYVLKKEDIANAMDCLEEALK
                     QYPGRR"
     gene            16094..16480
                     /locus_tag="PLM68_02070"
     CDS             16094..16480
                     /locus_tag="PLM68_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOT54190.1"
                     /translation="MKTLYTVLLLVLSNIFMTIAWYGHLKFASLKWFQSLPLIAIIVI
                     SWGIAFVEYCFQVPANRIGYLGNGGPLSLIQLKVIQEVISLSVFTIFALVVFRTEQFR
                     WNHILAFFLLVAAVYLVFRVPDNPGT"
     gene            complement(16537..16878)
                     /locus_tag="PLM68_02075"
     CDS             complement(16537..16878)
                     /locus_tag="PLM68_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOT54191.1"
                     /translation="MYFEDYDSSSKTEHRGEDVFSKPVRAGKRTYFFDVKSTKGNDYY
                     LTITESKRRVDKEGRFSYEKHKIFLYKEDFEKFAEGLEEVVTYIKDRIPVTESRPSEF
                     TDVSFDDLNKE"
     gene            16967..17911
                     /locus_tag="PLM68_02080"
     CDS             16967..17911
                     /locus_tag="PLM68_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HOT54192.1"
                     /translation="MLSRRFIRVKVFKILFSSVHSQNFDQAVAEKELLASFEKTRQLY
                     YLLLSLPVELANYAQSRIYIGLNKYQPTDEEINPNLRFVNNKAITILEKDKALVKARE
                     HGLNWALHKGYIKKLYEQFRKEPYFIEYMNAPQEPGFAEDLKLLQDFFSDQPEDDPDL
                     YTLLEDQSLYWADDIAYVCNIILAQLAGMKEGKDMPAHPSLFNNQEDKDFATELLAHS
                     LAHYKEFVGYIEKFAQNWDLERIAATDTILIVMGVAESVAFPQIPVKVTLNEIVEISK
                     YYSTDNSKMFVNGILDKIIKHLVQEGIIAKRGRGLVEK"
     gene            17955..18278
                     /gene="yajC"
                     /locus_tag="PLM68_02085"
     CDS             17955..18278
                     /gene="yajC"
                     /locus_tag="PLM68_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT54193.1"
                     /translation="MNTFVILQQTATSGATGGNWTFLIMMAAIFVVMYFLMIRPQKKR
                     QKELKEYRDNLKKGDKVITAGGIYGTIAEVKENYVLLEVDTNVKIRVDKSTLVKDSSD
                     IQQGS"
     gene            18308..19252
                     /locus_tag="PLM68_02090"
     CDS             18308..19252
                     /locus_tag="PLM68_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54194.1"
                     /translation="MKRSRWVLILCIVAAFFLWVVYTLSNEYTAYFRFPLEVETNLYG
                     RSNLSQADNTVFLRGKTRGFYIIHYTWRKKIPTLRIFADQALIHKYPDRKDKFYLLAG
                     EINNLVNDALGTDFQLETILSDTIRLEYREVHYRKVPVHFVSDVSYAPQYMSFEPVRI
                     FPDSVLVYAEEPYFDLIGEVRTRKVSLSDLNRTVEGIAHLETPANARLSHNEINYEVM
                     VTRYVELSTEVPVTTRNVPYRTELLLLPEKVILQYRCPFEDAGNITDEDFSLVVDYND
                     FIEKKGGKITPSLERAPVVISHVQLTPQYVDGIIQNRY"
BASE COUNT         5020 a         4610 c         4446 g         5265 t
ORIGIN      
        1 ataagattgg taatttttat atttcaatag cagataacgg attgcatact gcctttttaa
       61 aggatcacgt ttttatactt caggatcacc ttgcctgtgg ttgtggttta ctttctttca
      121 atgaaagaat aaggatagta aacaatcatt ttttttacag aacgtttatt ttgccaaacg
      181 gggacgtcta cgtctctttt gatacccaag ccgtagccaa tatgtacaga aatccaattt
      241 cttacgttaa ggaaacaatc aatctctttc atacagaaca atggaacaaa acctgggata
      301 acagtgtttg ttccaattgt ttatttaagt atatttgtcc attgctgtcc cggtacgatt
      361 ttccagaccc cttatttccc acaaatattg aaggtcatga gtaaatttat catagtcatc
      421 atcttctctt ttctgacggc aagtctttat ggacaagtta cggttacagg tcatatttca
      481 gcagaaataa ttgaaggagt agaagtatca tcaaaaatgc tctccggttt tgaggttgag
      541 cccttttcag aaaagaaaga actgggagaa atcatcttgt cctgcgatga aagcgtcgcc
      601 tgcaatgtga tcctggaatt cggaaaactt attgataagt acggcaacgg agttattctg
      661 gaaacctcgg ttgagggaga tgaaaacaaa ctgagattcc tggcaagtac caggaatctc
      721 agaggtaaaa aaacaaatct ttatgaaggg tctttttcgg tgatgctttt ttacgagtaa
      781 ttcttatttt tttacacctt tcatcatttc tttcaagtct tctgaagtca ttttccccgc
      841 aaggtagatg acactgaatt cattattctc atctgcgcag atatacaggt cggtgatgat
      901 atcttcctgc ttttgcagat atatgataac tttttcatcc ccatcttcca cttccaggag
      961 tacttcaaat tcaccggttt tgaccagatc agagaaatct tttttcatct ctgtaatgtg
     1021 ccccttgttt gaggtggaaa gaacataaag gccagtcata ttttttatca ttccggcaat
     1081 gttcacatct tcagtaaggt ccaattgcat gccatcgccc atcagggaga acatggaggg
     1141 ggatatatat acttttgtca tgccttcata actggagaat tttttataaa aacggctctg
     1201 accaaacgca gaaatggtca gcaaacatgt taaggcgact ataattaact ttttcataga
     1261 attatttgat tttgatatat ttattaactg tatttattgt agagatgttt ttatgcgtct
     1321 cagaaacttt gttaactcct ttattcatca gggaggatac atattgcagg acctcttctg
     1381 tttcctggta ggcatcctcg acatcggtaa aggtgtctgc tatgtaaaca ggcctgtcat
     1441 gtaaggggag cgatacggag agtgtaatga ttatggctac cgatgccgca acagccagcg
     1501 ctttccatag ggggatcctg cgtacaggtc tttccgctac tacctgggac tcttcagctg
     1561 ccagcctgtc tatggttgcc gataatcttg cttccaggtc aacgggaact tctatttcct
     1621 gatattgttt gtccatgatc ttttttatcc tatctgtttc catggcagtt gattctttcg
     1681 tattgttcct tcactttttt ttgtgccctt gacagttgaa cccttatgtt taccggactc
     1741 agtcccgtca gttgccttat ctcttccagt gataattcgt tgaaatgcct cattctgaaa
     1801 atctcccttt gtttttgtgg caactggtcc accagaacca gtaccatttt tagttcgtcc
     1861 tgctgatcta tttgttccac ggcatctatc cactcgtatc tcaaagattc agcagcagag
     1921 ttatctgaat ttctgtaaac agaggagcgc atcctgtcaa ggcacaaatt gcgggtcatt
     1981 gtggtggcat aagcctggag gtttttgatc ccggaaaggt tttccctcat attccaaagc
     2041 ttcacaaaga cgtcctgaac cgtatcttct gcatctgcct gacagttgag cagccggtaa
     2101 gccgtgcggt acaaatggcc atgccagggc aggaaagtat ccttgaaggt ttgtgcatca
     2161 aacattttta atacctatga ccagttcttg taaggattct gcagttgctt gttcgcttac
     2221 aaatttagta cttatctggt tattggattt ccatgtgaaa gccattacca gcagggctat
     2281 ggtcagaacc acaatcacca ccatgggata aagtccttct ctagtagttt caattgtttt
     2341 catttttata tgttttgtga tatcattttt tccatacagg aaaggtcaat actgccgttg
     2401 atctggatca ggcagggttc ttctcccacg ttgattacaa taaacctatg aattttgtca
     2461 ttacggactt ctgccagtat gcggagctga tcgctctcat cactaacttc tgcaatcaat
     2521 tccagtcctt tttcagataa tctttgagct tgttgtctga atgcctcttt aatgtttatg
     2581 tcacagcatt caagcgaaag tatgctcaca gatctgatgc cactgataaa ctgtgcgtca
     2641 tccttatcgg caaatatttc tgccagtctc atggcaaaac ttccgacatg aatatgttct
     2701 gcggcgggct ggcaggaaaa ttgctgcacc aaatcttcat aatctttttg cgcataagat
     2761 accagaacgg tcccggccag gagtaaacat gtgataaaaa tctttttcat ttgtgttttt
     2821 atttattgtt agttttcgat ctcactataa agacgtaaaa cccgggaaga tgttacagct
     2881 gtcgtgagaa tctttaactt tgtatcatga aagttctagt tttggggtcc ggcggccggg
     2941 aacatgccct tgcctggaaa ttggcccaat cccctttggt aagtgacctt tttgtgggac
     3001 cgggtaatcc ggggacctct tccctcactt taacaaacgg gaaacaggta cagaatgttg
     3061 cattagtaac taatgatttt cagtctgttg aaaacgtttt actccaatac gcgatagaca
     3121 tgttggtggt gggacctgaa gaacccctgg ttatgggaat caccgatttt tgcaaaaaca
     3181 gggaaggact tgaaaatttg ctggtcatag gacccgacag ccggggagcc agcctggaag
     3241 gaagcaagga tcatgctaag gcgttcatga cggcctgccg cattccaaca gccctttacc
     3301 gcacctttac ctgcgggcaa tatgccgaag cctgtcttta tctggattcg ctggaacctc
     3361 cttatgtatt gaaagccgat ggactggcag ccggaaaggg agttctgata gaacctgacc
     3421 tggtcaaagc ccgcgcaggc ctgaaagaga tgatggaagg aaaattcggg gcagcaggca
     3481 ataaagttgt ggtggagcaa ttcctgcggg gaaccgaggt ctccgtcttt atacttactg
     3541 acggcaccca ttataagata ttgcccgagg caaaggatta caagagaata ggggagcgtg
     3601 accagggtcc caacacggga gggatgggcg ctgtttcccc ggttcccttt gccgacagga
     3661 gcttcatgaa aaaagtggaa gaacgtatca tcaggcctac cctggaaggc ctggccagaa
     3721 aaggatgcga ttacagggga ttccttttct tcgggctgat caattgtaat ggagatccct
     3781 atgtgatcga atacaatgtc cggatgggtg acccggaaac agaatctgtg atgatgcggt
     3841 tacagggtga cctggccgcc gcctgctact ccctgaaaac taaaacactg gacaaaatgt
     3901 ccctatctgc tgcggaaaac cactgcgtta cggtggttgc agtttcaggc gggtatcccg
     3961 gaacttatca aaaggggtat gccataaagg gcctcgacca ggtgaaggaa gccaacgttt
     4021 tccacatggg aacatcaaca acaccccaga tcgtgacaaa gggaggacgt gtcctggcag
     4081 tatccgccac cggcccgacc ttgctatccg cccgggagct ggcgtaccgg gaattgtcac
     4141 tcattgctta cacagatatg gtttacagga atgatatcgg ggaagacctg atggatatga
     4201 aatattcatg aaatgatcca gttcataaag cgcagtgtat ggggagcagt cttggtatcg
     4261 ctactgataa tgactgtttt ccgtgtgttt tatcacttta gccaggaggt tgctcttccc
     4321 tggtatttgc atctgttatc ggctgccatg atcttggtaa caggaatgtt actgtcatac
     4381 ctgaacgatc gttacgacct cttgccggaa aggacacaat ggatcactgt agcttatgta
     4441 tggtttgttt cctgctgtcc acaaacttat gacaacctgt gggcccaggg agccgccctg
     4501 cttattgctg ccgctacata caagctggta tactctgcat acgttcctgt gacccgttcc
     4561 ggtcctttta tgtctgcctt tttcatatcc tgtgcaggat tattgttctt cccggctttt
     4621 ttcctgtata tcccattatt gataagcttc atccggatgg caaaagtatc cggaaaggat
     4681 ttgctggcct ttgcaggcgg gatgctttca cctcttttcc taacctcggc attgctctgg
     4741 ttcatgaaac atgacgtggc actgttctgg ggacacaggc tggaaaattt taaacaaatt
     4801 taccctgcaa aaacccttga agcattctcc atttcaggga ctgtgatggt tgccctgata
     4861 gccttacttg tcatgctgtc tttattcata agactggtcc ggcgcgatac taccacaaca
     4921 gtgaatgtgt cccgttttta cagctccatg ttctggtttg tcttattctc atgcctggtt
     4981 atgctcactt atacaggttt tgcatacgga tatattccaa tcatgatgat acctgtatcg
     5041 ctgctgatca catcactttt tactggaaaa cgtttctttg gatctgtttt gatgatctca
     5101 ctgctgatca ttgcgtcact ggtctattat gtctgccggg tctttctatt cccctattga
     5161 tcctgtcagg agtttgcctt caaaggttcc tgtgagggtt ctcagaaaag acacaacctc
     5221 tcttatctct tttagggtca aagtcttgtc tctctgatag aatgccatgg ttttaaccgc
     5281 tgtttctaga tcaggagcag acgagttatg gaaatatgga taggtcaaag ccacgttcct
     5341 cagtcccggt accttgaatt tgtaaatatc ttcctgcagg ccggttaccc tgtagcgccc
     5401 caggtcgggg agctgcacct tgtctcttga cgggaaaaat ttattggaca agcccatgta
     5461 ttccaaagag ttgccaccta ttgccacgcc attatgacaa cttgcacaac caattttctt
     5521 aaacaggtca taaccttttt tggcatcggg gcttattgcc tccccatccc ctttcaggaa
     5581 ttggtcaaat ggagcatccg gagttatcaa tactttgtgg tactcagcca gggcatcggt
     5641 gatgttttcg gcagaaaatc cttccgtgta cgttgctgta aataatgaag tgaagtcagg
     5701 atcggcttcc agcctgtctg ttatctcttc aaatgactcg tttcccatga tgacaggatc
     5761 agtcagcatt aatgccgatt gaagagccaa tgaagaaacg cgtccgtccc aaaagaaaaa
     5821 ctgttgaaaa gccgcattga aaattgttgt tgtagaatac ctcccgggct gtgcgaaagc
     5881 acccagggaa aaattcatgt tgtcagcacc tccttggtcc agcttgtggc atgttgcaca
     5941 ggataccgtg ctgtcagcag acaaacgcgg atcgttgaac agtatccttc ccaggttcac
     6001 tttggaaagg tttgccggca gggaatcagg taccggtatt atcggctcaa aagtacgttc
     6061 tttggtgggt acgggccagt cgtgctctgt tgataaatgg tcgtatagcc aatgcagcaa
     6121 gattttctgt ttatccttta ttatggccga tttccagtga atgatactat agctcaacgg
     6181 aggcatgctg cgctcagata tcactttctg catttttatg agtatggatt catctacggc
     6241 ctcatcatta tttaaatgca taaggactga aaaggcattg gcatagcgta ctcccttttc
     6301 agctttcttt tcaatcatgt gaccaaatac cggcacattg atattgggat agaagtcggg
     6361 gccgccatga caaacaaaac accctccttc ttctacaatc gtgacgacct gttcttgcac
     6421 tgaaagttta tttgaaggag caacattgat tacccgggca ataaaaaatc ccaggccgat
     6481 caggataata agggtgcctg taattttcca tagtattgaa agaaaatgct tcaccttaag
     6541 aaacctttat ttcgtaatta tgcaggcttc cgcgcgcctg tggcaggtat tgcagcccat
     6601 accagcagat ctgcagggag atgaaagcca atatcaaaga tacaaaaatc attctttttg
     6661 cattggggcg tatttctttt atatgaaagt acagggcata taatagccag gtaatggccg
     6721 cccacgtctc tttggggtcc catgtccagt aatctcccca agcctgtttt gcccataagg
     6781 cacccagcaa catgcctatg gtaaagaaag aaagtccggc cttgcacaaa gtatcgattg
     6841 ccggcagcac atcattggac ggtttcaaca ggcctgcaat ggccatcagg aatgtgcagg
     6901 ctagcagggc ataggagaac atatatacag tcacgtgcgg gacgaaccag atgctctgca
     6961 ggacaggcat cagaacacgg tcatgtatgc cgggacgaag aacattcact gcaacaaaaa
     7021 ccagggacat tactgtggtg agcgacagga tccatttata ccgccacctt atgtatgtta
     7081 taagcccggc aagggaaaga aacagagaat accagaggcg tgtctcaccc atggttacca
     7141 gtggcggccg gtgcagtgat atccaaagac cggtgatata ggcggcgtat atgacgacac
     7201 cggtggtgta tgctattatg gtccatacat accttttact gaacttcgcg atggccagca
     7261 acgatcctgc aatccagaag acagcggcga cagcagcgaa gatcagatga tgctcccagg
     7321 tcatagaata aaggttttaa atgcgcctcc cagcattatc agaatacctg ctaatacaag
     7381 ccatcgccag ggttgcctgg ttaccatgac tatacagtaa tcgctttcgc ttcctttcga
     7441 ggtatcgtaa tccataaggt ataactggtg cccccggaag tgaacagggt ggtttaccct
     7501 cagtgttttg gattgctccc tgtttgcgtc gcgcagtatg atctgtgccc tgtattccat
     7561 gggttctccc gtttggtagc ggtcaacctg gaattcgtcc atacgcatcc caaagggaag
     7621 cagggtctgt gtgaattcct ccctgggaac aaccactctt gtcaccgtag cgtctgcact
     7681 gcccagcatg ccggaccaca aggcaatcca caatcccaga cctgcccatg aaaattttcg
     7741 gaaaaggcgc gaggccagaa cagtcaaaaa ccaggttaaa acaattacaa aaggccagga
     7801 acgggtaaaa gatgcctgag cgggcatgaa accctgtacc agggcggcag cgatcatcag
     7861 caacagggca attgtcatgt gcctgtccga ggagaggcat ttgaacttcc aggcagctaa
     7921 gaccatggat aaaccaatga taaggtttac ggggaaagca aaaagcgcgg tattaatagt
     7981 catcttgcag cattaatgtt caccttgcgg atcgccttat ttgtacacaa atatagtatc
     8041 tttgctccaa tgggaaaatt aaatattgta gttggaaaga gcaaggacga aactttgaca
     8101 gaagaattga aaaggtccgg tttaccggtc gtgctttatg gcataggatt gattgcagac
     8161 actacccgga tctttttgca ggaaaaagga atccgtgttt catgccgtgt tgtcgacgac
     8221 aaatacctgg acccggctgc acaccatgcg gattccgacc atatcttgtc catttctcag
     8281 ttacagaagg agttcagtgc ctataacctg ctgctgtgct ttttcggagg atataagaat
     8341 gacctgcaac cataccgcca gctatttcca ggagcaagga tgatagactt tctctcttcc
     8401 atatatgaag ggggtgcact ccagtcaatg gaatcatctt atgtcctggc ccataaaccg
     8461 gcatttgaca gtgtttatga actgttggag gactccctgt cccaggacag catggaagct
     8521 tacatcaacg ccaaaatcaa caaagacgcc agttaccttt ttccctttgt ccgaagacca
     8581 cagtattttt ctgaaatcga ccaggtgccg gcactcaaac tacatcaaaa tgaagtcttt
     8641 gtcaattgtg gggcatttac cggagacacc atcaaagatt ttctggaagt aacttcctgc
     8701 catttcgaca aaatttatgc ctgcgaaccg gacagcgcca acctgcaaaa actcacccgg
     8761 tttatccagt ctgccgggat agcggagcgg acagagattg ttcccaaatg tatctcggac
     8821 aaagccggaa cagtcaattt cctgaaccag ggcagcatgc tttcccacaa gacagaggca
     8881 cccggtccgg gcgttgtggc catgcaggcc gataccattg acaacatcct gaattccagg
     8941 ccggcaagcc tgattgtgat ggatgtggag ggcaacgagc tcaaagccct ggagggagcc
     9001 cgtaaaacca tcctggcgca tcaacccctg ctggccctgg ccgcttacca taaaaaagat
     9061 gacctcccgg ttctcacctc tttccttaaa aacctgctcc ccgcataccg tttctacttc
     9121 agggtgcata aacccatggc cattgatgcc gtgctctatg ccagcacaag gaatgaaccg
     9181 gttcccgcat aaaaaaaccg gcccaaagcc ggtttgtttg aggtacccag cggaatcgaa
     9241 ccgctgtact cggttttgca gaccgatgcc taaccactcg gccagggtac catttacaaa
     9301 aggattgcaa agttagtaat tatttttaaa aagacctgtg agttcttcca gatactgcct
     9361 gtccatctgg tcaaaagtgt tgattttttc tgaatcaatg tccagaaccg ccctaacgtt
     9421 tctttccctg tcaaatacag gcaccactat ctcagaacgg gaacgcgaac tgcaggctat
     9481 atgtcctgga aatttttcca catcggggac tatgattgtc ttacgctgtt gccagcaggt
     9541 gccgcataca ccccgtccaa agggaattcg ggtgcaggct gcaggtccct ggaaagggcc
     9601 cagtatcaat tccttagctt tgctgtccac ccggtagaac cctacccacc agaagttgaa
     9661 cttttctttg atcagggcgc ttacgttggc catattggct acatgatcag actcatcccg
     9721 gagcaacgaa tcggcagaaa ccaggatttc ttcgtacatc cgctgtcggg gcgttttctt
     9781 ttcattgctg tgtgcaataa gcttattgta aatacaatag catacgtaag catcagttgc
     9841 agcatagatg gcctgggcgt tggacagggg gtaggcttcc caattggtaa tttgctggga
     9901 tttgctgata cgttttccca ggatgatggc agacattttc ttgacgctct tttccactat
     9961 gccatactcc cggaccatat cctggaggtc tgtgaaacct gccggttcaa aagaggcata
    10021 gcgttgaagc cctgctatgt cgtccttgat cgcggcacct gccttgatga tagcagggtt
    10081 gctcagaatg gcctggatct ctggcggcag cccggttttt tttaggtgga tcaggaatgc
    10141 acggtttccg tccgagagct gcaacagggc aggcatgtac atcctgcccg aagaaaaacg
    10201 tggccttgtt tctgtatcga atcccaggac aggttgatcg gaaaggtact gtacagcaaa
    10261 cagaacctgg ctttttgtcg tcactgttgt tatctgtcct ctgaattcca cgtggggaag
    10321 caattccagg acattgttct caattgttga tgcaaacatt tagcagcggg tattttgtca
    10381 gatcgggttt ttcaatttca tagaccctca cacaggaagg cgtggtacgg aaggcagcca
    10441 gtcgttcttc cctcatggcc ctgtatagtt gtattccggc ttctttctgc ggttcaatca
    10501 ggatcatgtc ggaatctaaa agatccctgt acacatagcg gtcatccttg cggtaatttc
    10561 tcaggaaagt aagtaattcc gggatcaact cacggtgctg cagcagcggg tgagctccca
    10621 ggacaatata tttcatacgg gcctggttgt taaccggcag cttgtctgcc agtgaggtca
    10681 tggcaaaacg gatggctttt tctgtggaat ttgtctcctt atcttcatat gcctggaaaa
    10741 caacctgggt gacatctgcc aggcaatcgt aggcatttat ggaaaaagcg ccgtctgttg
    10801 caataacacc caccgtcaga cgtttgtccc tgccagcgtt gttcttgtgg acatattcca
    10861 gtgccttgtc gttgccggct tgtatgacac ttaccgttat aatccccagg ttacccattc
    10921 caatcaggtc tttcaggtca tcataaactt cagggggtac aaggtcacct gccactacca
    10981 gcgcttttac aggaggcctt tggtcttgtt ggcggaacaa ccggacaatc tgccttatca
    11041 tgtcttcccg ggaaagggtc tcaaaagaag gaaggcagag aaattcaaaa tgttcgccgg
    11101 caaaatccct gattccatcg gaaccgggct ttccggtaat gttgtcaaac tcatcaagac
    11161 ccagcagtgt ctccaccacc tgcagatcat cggctgaatt tccgatgacc ccaatgggaa
    11221 gcattttcat actttgggaa tagccaaggg ggtctatgta ataagccgag gcggtatctt
    11281 ccagcatctc ctggatgacc ggcaacggag gtgcgacttc ctgtttgcca tcaccgcagc
    11341 ttccggccag gagacctgtg cccatgagca acagcaacac catatttctg aaaagatcag
    11401 tcattcttgt catatgtaaa tgtcccagcg ggactttcaa cactcaccct gcgccccgtc
    11461 cagccctcaa cccttccaat gacccgcgcc tccaggtcaa atgctgctgc catggaacac
    11521 agtgcaggag cgaccacagg aggaacatac aattccatcc tatgtcccat attgaaaaca
    11581 gtgtacattt ctttccaagg agttccgcca atttcccgga tcctgctgaa cagggcaggt
    11641 accggaaaga gattgtcttt gatgacgtgc aggttgtcaa tgaaatgaag cactttggtc
    11701 tgtccgcctc ccgtgcaatg taccatgcca tgtacctggt tgcggtattt acccagtatg
    11761 gctttgatga ccggggcata ggtgcgggta ggggatagca ccagcttgcc ataagtcata
    11821 cctgtttcgg gctcgatatc agtcaggtcc atggtgcctg aataagtcat ctcgtcgggt
    11881 atgcccgtat cataactctc cgggaacatg cgggccactg ctttgctgaa aacgtcgtga
    11941 cgggcacctg tcaatccgtt gctgcccatc ccgctgttgt atgcatcctc atacgttgtc
    12001 tgcccaaagg aagacagtcc caccacaacg tctcctgctg tgatacgggc attgtctatc
    12061 acctggtcac ggcggatgcg tgcacaaacg gtactgtcaa cgatcaccgt acggacaagg
    12121 tcacccacat cggctgtttc ccctcctgtg agttcaagcc gtatcccata caactgcatt
    12181 ttgtcgcaaa aatgcctgct gccctgtatg atggccctta tgacttcccc gggtaccagt
    12241 cgtttgttcc tgccaatagt agagcttagg agcaaatcgt tagtcacccc cgcgcagagc
    12301 agatcgtcca ggttcatgac aatggcatcc tgtgcaatcc cttcccagac gctcaaatcg
    12361 ccggtcttct tccagtagca gtaggccagt gacgatttgg tgccggcacc gtctgcgtgc
    12421 atcaacaggc aatgttcccc gtctcctgtc aggatatcgg gtatgatctt gcaaaaagct
    12481 tttggataca atccgcggga caatccttcc agggcatcgt gcacttcgct cttggaagaa
    12541 gacaccccgc gcttgctgta ttttccggct tcaaggtcgt tcatgatgtg cttgtcaagg
    12601 ttcatcgcag gaacagcagt tccctgtatt taggcagtgg ccacaattca tcatccacga
    12661 tcatctcaag atgatcggca caatcccgga tgcgatccat tacgggcaaa acggcagcag
    12721 catactcccg ggccctgtca tgcaacaatt ccacattatt ggctttcttg cgcgcttcga
    12781 tcaaaagatg aaccagcccc cgcatctcgt tcacatagcc ggcgatgatc cttatggctt
    12841 tcaactgggt agaaacaagt tcctggtact cttcaggcgg gaacaagtca cggagacctt
    12901 tcacattttc cagcaacatg ttctcatgac ggatggcagc aggcacaaaa tggtttatgg
    12961 tcaggtctcc cagcacccgg gcttctatct gcagtttttt gacatacatc tggttcctaa
    13021 cctcgtacct ggcttctagt tcacgacggg tcatcacgca catttcctcc atcagggcta
    13081 tgctttgttc ctccagcaag gcatcgtaag aagccggcac atccgttatc ccccgcaatc
    13141 cgcgctctgc tgcttccttg tgccattcat cactgtatcc gtttccttca aagcggatat
    13201 cccttgattc cctgatgaat ttgcgaatga ttttcatcag cgcatcctcc agtttttctc
    13261 ctttttctat ttccttgtca accaggacct tgaatgattt caactgctga gccaccgcgg
    13321 tattcaaaaa gagcatggcc gagcccgcat ttgccgaaga gcctatggcc ctgaattcga
    13381 aacggtcacc agtaaaggcg aaaggtgagg tacggttcct gtctgtattg tcagggaaga
    13441 tctcgggcac catgctaacc acgtccatgc gcgtcctttc actgctcctg ccccggcccg
    13501 ggcccccctt gtcaatcacg tccagcaaat cggcaagggt gctccccatg aatgcggaaa
    13561 gcaccgccgg aggtgcctcg gtccctccaa ggcggtatga attgctgagg gttgccaccg
    13621 cggacattat caggaaatgg tgttggtgaa cggctttcag ggtacaggca atgaagctca
    13681 ggaattgcag gttgtcctgg ggtgttttcc caggggacaa aacgtttatt ccattgttgg
    13741 tgaacaggct ccagttgcag tgtttgccgg aaccgtttat gcctgcgtaa ggtttttcat
    13801 ggaatatgac tttcaggttg tgctttttgg caagttttct catgaccagc atgatcatct
    13861 ggttatggtc aacggccaga tttgcttcct cgaagtaggg agcaaactca aactgattgg
    13921 gagccacctc gttgtggcgg gtttttatgt atacgcccag tttataggct tctgtctcga
    13981 aatctttcat gaaagccatc acccgggaag gaatattccc gaaataatga tcttccagtt
    14041 gctggtcctt ggcagcggtg tgtccctgta acgttctgtc gcatagcatc aaatcaggac
    14101 gggcacggta cagcgccaga tctaccacaa aatattcctg ttccagtcca tagatcacat
    14161 tcacgctttc cgtttcggga tcgaaatacc ggacaacagc agtggcagcc tggtcaaggg
    14221 cacggaggga tttcaggtgc ggggtcttga catccagggc atcaccagta taggaaacaa
    14281 agatggtcgg aatgcagagc gtctggtcca ggatgaaagc aggcgagccg ggatcccatg
    14341 ccgtgtatcc gcgggcttca aaggtattgc gcaatccgcc gctgggaaaa ctcgatgcgt
    14401 cgggctcctg ctggatgagc gcatcaccca cgaattgctc aatggcgcaa ccgtttgcat
    14461 acggacggat gaaggcttcg tgcttttctg ccgttgtctc attcaggggg tgaaaccagt
    14521 gcgtgtaatg ggtggcgccg cgctccatgg cccaggaacg catgccttca gccacatgcg
    14581 tagctacgct cctgtcaatc ttgcacttct tttcaatggc tttgaccaca gcttcatacg
    14641 cttcctcggg aaggtaattt ttcatttttt tcaaatcaaa cacatgttgt ccgaagaaag
    14701 aagatacagg tttgttctcc acacagaaac gttctgcctt atgtgatgaa aattccaaaa
    14761 gtgctttctg cctgaatgtt gacatctttg ataaaacaag tgatcagcaa aaataggaaa
    14821 atattattgt cttttgtttt aataaagact tatctttgtg gattaaattt caaaaattaa
    14881 ttctcactat gttaggtata tctgaattgt caagaaaaat gccggcatct cccatcagaa
    14941 agctggttcc ttatgccgaa gcggcaaaaa aacgcggaat caaagtgtat catctcaata
    15001 tcggccaacc cgatatcgat tcccccaaag aagccctgga gccgatacgc aatattgacc
    15061 tcaagcttgt ctcttacccg cattcagccg gtattgaatc ataccgcagg gggcttgcaa
    15121 aatattacca ggatgtgaac atcaatctca catacgaaca gatcatcgtc accattggcg
    15181 gttcggaagc cctgcagatc ggcctgaagg tatgttgtga ccctggagat gaagtcatag
    15241 tttttgaacc tttctatacc aattataaca cctttggcct aatggcaggt gtcagattcg
    15301 tacccgttac gaccaccatt gaaaacggct ttgccctgcc tcccatgtcg gaagttgaaa
    15361 aatatattac gccaaggact aaagccatcc tgataaacag tcccggtaat cccaccggaa
    15421 ccctttactc aaaggaagac atactgcaac tgggtgagat tgtcaagaaa cacaacctgt
    15481 tcctgctttc agacgaagtg tacagggaat tctgttattc ggacgagcct catttttcat
    15541 gcatgaacat acccggagcc gagcagaatg ttatcctgat agactctgtt tcaaaacgtt
    15601 acagcctttg tggcgtacgt atcggatgca tcgcttccca caataaggat gtgatgagcg
    15661 ccgtattgcg ttttgcccag gcccgcctgt gtgctcccgc cctgggacag attgcctctg
    15721 aaggtgccct ggccaccccc cgggaatatt ttgaagctgt aaaacaggaa tatatcgaca
    15781 gacgcgacct gatgatagaa cgattgaaca agatccccgg ggtgtattcc cccatgccca
    15841 tgggagcttt ctatacgact gcccgcctgc ccatagatga cgcggacaaa tttgctcaat
    15901 ggttgctgga aagcttcaat tacaaaggtc aaaccgtcat ggtggctcct gctgccggat
    15961 tttatgccac ccccggaaaa ggccttgatg aagtccgtat tgcatacgtc cttaagaagg
    16021 aagacatagc caatgctatg gattgcctgg aagaagcatt gaaacaatat ccgggacgcc
    16081 gctgagcggt gcaatgaaaa cactatacac cgttttattg ctggtgctgt ccaacatatt
    16141 catgaccatt gcatggtacg ggcacctgaa gtttgcatcg ctgaagtggt ttcagtcgtt
    16201 acccctgatt gcgataatcg tgatcagctg gggcatcgct tttgtggagt actgtttcca
    16261 ggttcccgcc aaccgcatag gttacctggg aaacggagga ccgttatctc tcattcagct
    16321 gaaagttatc caggaagtga tctcactcag tgtattcaca atttttgcac tggtagtatt
    16381 cagaacagaa caattcagat ggaatcacat cctggctttt tttctgcttg ttgctgcagt
    16441 atatctggtg tttagagtac ccgacaaccc cgggacataa taaaaccgca ttgttaaatg
    16501 cggttaagcg taaggtaagg catccctggg ggtgccttat tccttattca ggtcatcaaa
    16561 acttacgtcg gtgaattcag agggtctgga ctctgtgaca gggatcctgt cttttatgta
    16621 tgttacgact tcttccagtc cttcggcaaa tttttcgaaa tcctccttgt agaggaaaat
    16681 cttatgcttt tcgtatgaga acctgccttc cttatcaaca cggcgtttgc tttctgttat
    16741 ggtcaggtag taatcgtttc ccttggttga ttttacgtca aaaaaatacg ttcttttacc
    16801 ggcgcgtacg ggtttggaaa aaacatcttc tcccctgtgt tccgtcttgg aggaactatc
    16861 gtaatcttca aaatacatag gtgttattta tttaaacaac gcaaatataa aatatattta
    16921 ctttaaaact gtatatttgc agagtttctt tttgatttgt aaagatatgc ttagcaggcg
    16981 gtttattcgt gtcaaagttt tcaagattct attcagcagc gttcattcac aaaattttga
    17041 ccaggccgtc gctgaaaaag aactccttgc cagctttgaa aaaacaagac aattgtatta
    17101 tctgctgcta tcccttccgg ttgaactggc taactatgca cagtcacgca tttatatcgg
    17161 attgaataag taccagccta cggatgaaga gatcaatccc aaccttcggt ttgtcaacaa
    17221 caaggccata accattctcg aaaaggacaa ggcacttgta aaggcacgtg aacacgggct
    17281 caactgggct ttgcacaagg ggtacataaa gaaattatat gagcaattca ggaaggaacc
    17341 atattttatt gaatatatga acgcaccaca ggagcccggt tttgccgaag acctgaaatt
    17401 actgcaggat tttttcagcg atcagccgga agacgacccg gacctctata cgttgctgga
    17461 agaccagagc ctgtactggg cagatgatat tgcatacgta tgcaacatca tcctggcaca
    17521 actggccggg atgaaagaag ggaaggatat gcctgcccac ccgtcactgt tcaacaacca
    17581 ggaggataaa gattttgcga cagaattgct ggcccattcc cttgcccatt acaaggaatt
    17641 tgttggatac atagaaaaat ttgcccagaa ctgggatctg gagcgtattg ctgccaccga
    17701 taccatactt attgtcatgg gagtggccga gtcagttgct tttccccaga tacccgtaaa
    17761 agttaccctc aacgaaatag ttgagatatc aaaatactac agcacagata attccaaaat
    17821 gtttgtcaac ggcattctcg ataagatcat caaacatctg gtacaggagg gcataatagc
    17881 aaaacgtggc aggggacttg tagaaaagta agcacagcat agaacaacaa aattttaaat
    17941 aacatttatc aacaatgaac acatttgtaa ttcttcagca aacagcaaca tctggtgcta
    18001 ctggtggcaa ctggacattt ctcatcatga tggcagccat ttttgtggtg atgtattttt
    18061 taatgatccg cccccaaaaa aaacgccaga aagaattgaa agaatatcgt gacaacctga
    18121 aaaaaggaga taaagtgatc actgcaggag gcatttatgg caccatagcc gaggtgaagg
    18181 aaaattacgt gttactggaa gtggacacca acgtaaagat cagggtggat aaatcaacct
    18241 tggtaaagga ttcttccgat attcagcaag gaagctgacc tgataacgcg tccccaaagt
    18301 atggtctatg aaacgctcca gatgggtatt gatcctgtgt atagtcgcag ccttcttcct
    18361 gtgggtggtg tacacattgt ccaatgagta caccgcttat ttccgttttc cactggaagt
    18421 tgaaaccaac ctgtacgggc gctccaacct gtcccaggcc gacaataccg tgttcctcag
    18481 gggaaaaacc aggggctttt acataatcca ctacacttgg aggaaaaaga tacctacgct
    18541 aaggattttt gcagaccagg ccctgataca taagtatccg gacagaaagg ataaatttta
    18601 cctgctggca ggagaaatca acaacctggt gaacgatgcc cttggcaccg attttcaact
    18661 ggaaaccatt ttaagtgaca caatacggct ggaatacagg gaagtccatt acaggaaggt
    18721 cccggtacat tttgtatcgg atgtatcgta tgctccgcag tatatgtctt ttgaacctgt
    18781 gcgcattttt ccggacagcg tgcttgtcta tgcagaagaa ccttactttg accttatcgg
    18841 ggaagtcagg accagaaaag tttctttatc cgacctgaac cgtactgtag aaggcatagc
    18901 ccatcttgag actcctgcaa atgcaagact ttcgcataat gagatcaact acgaagtcat
    18961 ggttacccgc tatgtggaac tatccacgga agtgccggtg accacaagga atgtgcctta
    19021 ccgcaccgaa ttgttgctcc tgccggaaaa ggtcattctg caataccgat gcccttttga
    19081 agatgcaggg aatatcactg acgaggattt cagcctggta gtggattata atgatttcat
    19141 agagaaaaag ggaggaaaga taactccgtc actggaaagg gctcccgttg tgattagcca
    19201 tgtgcaactg acaccgcagt acgtagacgg catcatccag aatcgttact aataccatga
    19261 aacaaccatg acgtaaaaga cgtccgagcg aatgaaaaag aggtaatcgt atctttgtta
    19321 ggaataaaac aaaagaacga t
//
LOCUS       DAOSOF010000019        19190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2872_length_19190_cov_5.027541, whole genome shotgun sequence.
ACCESSION   DAOSOF010000019 DAOSOF010000000
VERSION     DAOSOF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2872_length_19190_cov_5.027541"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLM68_02095"
     CDS             <1..1222
                     /locus_tag="PLM68_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT54195.1"
                     /translation="KISEVLKVGQPVLVQIVKEPISTKGSRLTSDISIAGRNMVLLPF
                     SEKINLSSKIRLKEERSRLERLVQSILPPNYGLIVRTAAEGKRAAVLDTELNSLIHKW
                     EESWSVMRKGKIPSRLLTENSRITTVIRDLLNDTFTSIHVNDSMVFEEVCSYISTIAP
                     EKEKIVKLYKGKELIFDAFDINRQIRSSFGRVVPLRQGIYIIVEHTEALHVIDVNSGI
                     RSKMASGQEENAFEVNMVVAEELARQLRLRDLGGIIVVDFIDMDNADHRLKLYKKMQE
                     LMASDRAKHSILPLTKFGLMQITRQRVRPAMEINTSEKCPSCNGTGKIAPSLLFTENI
                     ENQLAFFANEKKLKSVILEVHPFVEAYLVKGFVSRAKKWSRKYGCRLNVRISPELAVT
                     QARWLDDNNEKIEL"
     gene            complement(1219..2931)
                     /locus_tag="PLM68_02100"
     CDS             complement(1219..2931)
                     /locus_tag="PLM68_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOT54196.1"
                     /translation="MNAENTQTSEERPLNFIEEIIQEDLAQGKHKSIITRFPPEPNGY
                     LHIGHAKSICLNFGLARKFGGKTNLRFDDTNPIKEDTEYVDSIKDDIQWLGFQWANEF
                     YASDYFEQLYQWALVLINKGLAYVDDQTQEEIRLNRGTITTPGTDSPWRNRSIEENTN
                     LFTRMRNGEFPDGSRVLRAKIDMSHPNMLMRDPLMYRIIHAEHHRTGNAWCIYPMYDY
                     AHGQCDSIEQITHSVCTLEFDVHRPLYDWFIEKLEIFPSHQYEFARLNITHTVMSKRK
                     LLELVQTGLVNGWDDPRMPTICGLRRRGYTPSGIRLFSEKVGVAKRDNVIDISLMEWC
                     IREELNKTAPRFMAVLDPLKAIITNYPEGAEEFMKAPLNPEDPGSGERLIPFSREIYI
                     ERDDFMEDPPKKYFRLRPGGEVRLKYGYIIRCNDMIRDQEGNILELHCTIDPDTKPGT
                     GTWRSVKGTIHWLSAKHTVAGEVRLFDRLFLEPYMGSIPEDKDYREYLNPESLKIIRA
                     FIDSQAVAMANQAVSGQDGSRIRFQFERLGYFVADYDTTKEKPVFNRICTLKDTFSKT
                     LNQL"
     gene            2997..3875
                     /locus_tag="PLM68_02105"
     CDS             2997..3875
                     /locus_tag="PLM68_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT54197.1"
                     /translation="MIRSMTGYGKAEALFSGKKFTVEIRSLNGKTADIGFKTSQIPRE
                     YEHDLRKNLINRLQRGNIDLFIAVDQEVADLDNIFNVELIKQYYKELQAIFRDIAQEV
                     SPSELAVSLLRLPEVREVQKKEVDIAEWQVLEQCIYQAADKLDEFRCREGEILGQDLL
                     SHIDNIESHLVKVGELDPERIVSVRKRLEDKLAGLGEDASIDKNRVEQELIYYLEKAD
                     ISEERVRLSQHCTYFRQTIVQEEYAGRKLNFIAQEMGREINTLGSKAAHAGMQQYVVM
                     MKDELEKIKEQVLNVL"
     gene            4012..4242
                     /locus_tag="PLM68_02110"
     CDS             4012..4242
                     /locus_tag="PLM68_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54198.1"
                     /translation="MNRTYSLKNEADTAYTDAQGKYHVSLTVSLRQRIFLVDYSDPRL
                     KYRDTLRRFTFEDQGTLYDTMSLKSRNQVDPF"
     gene            complement(4261..6369)
                     /gene="recG"
                     /locus_tag="PLM68_02115"
     CDS             complement(4261..6369)
                     /gene="recG"
                     /locus_tag="PLM68_02115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT54199.1"
                     /translation="MTSVWDTNIQFLQGVGPGRARELEKGLNIKTFGDLIRHYPFRYI
                     DRTRFYSISEIDPDLAAIQIRGEVRDMKIVGAGSRKQRLVVLFADPTGTVDLVFFKGI
                     RWIKDKIKVNTEYIVFGKPTLFNGRMNLVHPDMDIPDDQSLRGSSGLQAVYSSTESLR
                     DKGFTQKVFSRLIAQALNRVEGQVVEPLTPLMLKQLKLMPLAMALEEIHFPKNLKNLQ
                     AAQTRLKFEELFFIQLGLLKQRAVRLRAEDGLRFSTVGESFNNCYKRLPFELTSAQKR
                     VIKEIRADLGSGRQMNRLLQGDVGSGKTLVALLSSLIATDNGYQACMMAPTEILANQH
                     YNTVSRFLKGQNIKVGLLTGNTKASERKALLESLQDGSLHLLIGTHALIEDTVTFKKL
                     GFVVIDEQHRFGVEQRARLWEKTEGSPPHILVMTATPIPRTLAMTLYGDLDVSVIDEL
                     PPGRQPVQTIHYPSSKRGAVYSFLKKQITEGRQVYIVYPLIRESEKMDYENLETGYRD
                     ITMAFPAPLYSVAVVHGQQKPEDKAYDMNMFVRGHAQILIATSVIEVGVDVPNATVMV
                     IESAERFGLSQLHQLRGRVGRGAEKSYCILMTGNALSKEARQRLKLMCESSNGFEIAE
                     ADMRLRGPGDLEGTAQSGLAFDLHIASLAKDGQILEWARSEAQKILDDDPLLASEKNK
                     MLRLQLEILGKRRADYSKIS"
     gene            6410..6787
                     /locus_tag="PLM68_02120"
     CDS             6410..6787
                     /locus_tag="PLM68_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOT54200.1"
                     /translation="MSILGNILWLLFGGIMIALEYMVAGVLMCITIIGIPFGIQSFKL
                     AAFALWPFVKTTRTVESGTGCLSTVLNILWIFLGGIWIALSHLFWGVVLCITIIGIPF
                     GKQHFKMVTLAFTPFGREIIYKN"
     gene            6794..7828
                     /gene="pdxA"
                     /locus_tag="PLM68_02125"
     CDS             6794..7828
                     /gene="pdxA"
                     /locus_tag="PLM68_02125"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOT54201.1"
                     /translation="MHKKIIVGLTQGDTNGIGYEVLIKALADSRINELCTPVIYGSSR
                     LLGYYKKLCAGVENFNVHITQAASELHFKMVNLIPCVSDQFIAEPGKMTPEGAQAAMQ
                     SLGRAVEDLRGGKIQVLVTAPFNKQGVNREGFVFPGHTEYLANAFGVSNPLMMLCSHG
                     LRIGVVTGHIPLSRIPAELTKDKIVKKIKLMDSTLKKDFTIMQPRIAVLGLNPHAGDG
                     GLLGTEEEQVILPAIEEVNRSGILAFGPYPADGFFADAGYRKFDAILAMYHDQGLVPF
                     KALSFRQGVNFTAGLPVIRTSPDHGTAYRIAGEDKASAESMLAAIFMACDIYRTRRRN
                     QAITEGLHEG"
     gene            7818..8330
                     /gene="rnhA"
                     /locus_tag="PLM68_02130"
     CDS             7818..8330
                     /gene="rnhA"
                     /locus_tag="PLM68_02130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOT54202.1"
                     /translation="MKVNIYTDGSALGNPGPGGYGVILQSGRHYKEISAGYRLTTNNR
                     MELMAVIAGLEALKKHGTDVCIYSDSAYVVRAVEEGWLQNWKARDFCRKKNVDLWKRY
                     LDVASKHKVRFVWVKGHAGHPQNEKCDSLAVAAASAPVETLLEDQGFIDGLSLSPAGH
                     LDELPGWRND"
     gene            complement(8263..9390)
                     /locus_tag="PLM68_02135"
     CDS             complement(8263..9390)
                     /locus_tag="PLM68_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HOT54203.1"
                     /translation="MKTFTILLLFCLLISGCKSENNGEDPAMRDITSMELSKLMGIGW
                     NLGNSLEATGGETSWGNPKVTSDLIRAVKEAGFTSVRIPVSWSVHITDQGTHKIDNAW
                     MQRVGQVVNYVLVNDMFAIINIHWDNGWMNDPRESSRQQVNAKLHAVWTQIATYFKDY
                     DDRLLFAGSNEVHMEGDWSSSPPAEYLSVQNSFNQTFVDAVRETGGRNLKRHLVIQSY
                     LTNIDLAYRSLIIPQDPTPNRLMVEVHYYDPYEFALKEDEPYDFRWGSVATGPVTSWG
                     QEDWVESTFGKMKKFTDKGFPVILGEYGALYRIQLTGDELKEHKKCRSHYLEYVTEAA
                     IRHGMVPFYWDNGAPDFVLISRSSNQVVHPDALQAIMKARQ"
     gene            9435..>10835
                     /locus_tag="PLM68_02140"
     CDS             9435..>10835
                     /locus_tag="PLM68_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT54204.1"
                     /translation="MKKCFFILITGLLLISCTRTLLVDSNSVTVTVSDKSKVRIQPVS
                     PQIIRVSAVPAREKFSARPSLMRVPGETPLVPFDCLEEGQRVIVKTEALQASVNIHTG
                     EVVFMDPEGNVILQESQGGGKSFTPIEVEGDKGYSIRQVFESPADEAFYGLGQHQSDE
                     FNYKGKNETLYQYNTKVSVPFILSSNHYGLLWDNNSLSRFGNPEDYKQLDALDLRDID
                     GQEGALSAVYASRDGKTEYLRRRESRVDYTDLDKVKNFPEGIPFHNAQIIWEGTIGAK
                     ESGIHHFLLYYAGYTKVFFNGEEVVGERWRTAWNPNSYKFTAEMEAGIEYPLRIEWVP
                     DGGVSYLGLSLYTPVDPLEQEKQSWWSEMADMIDYYFIYGQDSDQIISGYRLLTGKAQ
                     IMPKWAMGYWQSRERYKTSDELLGALNEYRKRRIPIDNMVLDWFYWPVDSWGSHEFDK
                     ERFPDPKAMVDSVHAQN"
     assembly_gap    10836..10845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10846..12273
                     /locus_tag="PLM68_02145"
     CDS             <10846..12273
                     /locus_tag="PLM68_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT54205.1"
                     /translation="NARIMISVWPKFYHTTEHYKEYEDNGWMYTRAVEDSIRDWIWPG
                     YIGSFYDAFAEGARRLFWQQMEDHLYPLGFDAWWMDASEPDILSNASLEYRKALSTPT
                     ALGPSARYLNAYSLVNAQAIYEGQRGSDPDRRVFLLTRSGFPGLQRYSTATWSGDIGT
                     CWEDMKAQISAGINFSISGIPYWTMDVGGFCVQGRFERAREGSPDMEEWRELNARWYQ
                     FGAFCPLFRSHGQYPYREIFNIAPENHPVYKTMVQYNKLRYRLMPYIYTLAGKTWFED
                     YTIMRGLIMDFAEDENVINISDQYMFGNALMVCPVYEYKARKRDVYLPRQNDWYDFHT
                     GEYLRGGNFLTADAPLDKMPVYVKAGSILPTGPEIQYTCEKPGAPITLCVYTGADARF
                     TLYEDEGINYNYEKGFFSNIPFHYDQETGIFTIGARKGSFEGMPESREFHIRKITPHK
                     PGTPFAGGESLTVVSYDGKEQIIQL"
     gene            12279..14765
                     /locus_tag="PLM68_02150"
     CDS             12279..14765
                     /locus_tag="PLM68_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT54206.1"
                     /translation="MKRLYIIAAALLILGSCHRDSLRKVTDFNDGWEFARTGAIDDSL
                     IWESVDLPHDWSIEGPFDKNHPATPGGGALPGGKGIYRKVFTLEPGTEGKRVFLEFDG
                     IYRDSRVFVNGHLAGHRPCGYASFSYEVTPLLNPPGEENRVEVTVDNSLQPNSRWYTG
                     SGIYRNVRLVVTPEVHIPYSGTFVTTPQVSTDLAQVRVRTTIVRPFAATGDFYLLTKI
                     VDPSGLTVAKECNYAEPSPGGEIILEQTLEVKKPALWDVENPSLYTVESQLLRGGAVI
                     DNYSTTFGIRTFRFTADSGFYLNDRPFKIKGVCMHHDLGALGAAVNRRALERQLEMLA
                     QMGCNAIRTSHNPPAPELLDLCDSMGFLVMNEAFDVWRKNKTAYDYAMFFEEWYEKDL
                     RDFILRDRNHPCVVMWSIGNEVLEQWNNPVADTLDLQQANLLLNFMAGDDTQVQGDLP
                     FDALLTRKLARIVKELDPTRPVTAGCNEPAPYNNLFRAGELDIIGYNYHESDYAKVPV
                     NFPGKPFIAAETTSSLHTRGFYQMPSDSVRKEPKQWWMTYDTPHHMCSAYDNMCAPWG
                     NTHESAWMQVRDNNFISGMFIWTGFDYLGEPTPYWWPARSSHFGIIDLAGFPKDIYYM
                     YQSEWTDTPVLHVFPHWNWNPGEMVDIWAYYNQADSVELFLNERSLGISRKDSLKLHA
                     CWRVPFEPGTITAVSYSEGKEFLRRSISTAGEPASLKLTSDRVVLETGGEDLAFVTVE
                     ILDEQGNPVPYADNMVTFRLEGPATIAAVDNGSPISHEPFRADYRKAFHGKCLVVIRS
                     GKTAGKIRLTALSESFAQESTLDLETRLVQ"
     gene            complement(14709..16043)
                     /locus_tag="PLM68_02155"
     CDS             complement(14709..16043)
                     /locus_tag="PLM68_02155"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT54207.1"
                     /translation="MKEIITIRENSAIGFIPGDTYPLWQEKALRALDLLETGKGKGSD
                     FLGWLRLPSQTAPTVYEQIKDICLDWKDIELVVVIGIGGSYLGARAVIEALSHSFSAL
                     MPSSTPYIVFAGQNIGEDYLAELEDLMAVKSSACVVISKSGTTTEPAIAFRLVKQHLL
                     SRYGKEETRRRIVAITDKSHGALRKMVNSEEYRSFIIPSDVGGRFSVFTPVGLLPIAL
                     AGIDIQELMEGARDMEKITALKTGNNPAVRYAALRNALYSTGKKIELLVNYHPKLHYV
                     TEWWKQLFGESEGKEHKGIFPAGVDFTTDLHSMGQYIQDGERHLFETVISIAAPNRSL
                     AIPADEGDNDGLNFLAGKRIDHCNKMAELGTRLAHTDGGVPNLRIDMSSLNAYNLGAL
                     LYFFQKACGISGYILGVNPFDQEGVEAYKKNMFALLDKSGFEVQGALLRKRL"
     gene            complement(16047..17057)
                     /locus_tag="PLM68_02160"
     CDS             complement(16047..17057)
                     /locus_tag="PLM68_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOT54208.1"
                     /translation="MKGLFKKNTKYAIIGSGSWATALVKILLDSQDEVLWFVRDQNMI
                     NFIDQDKHNPHYLQAVELDTSRLLMSCDINKVISQGDVLIFCIPSTYFISELIPLTVP
                     LDEKFIVTAIKGLIPERNISVTEYFNRIHKIPYDNMGVIAGPVHAEEVAMERLSYLSV
                     TCKKESTAKKLAGVFRCPYIRTSIGTDIYGLEYSVVLKNIYAIASGMSRSLGYGDNFQ
                     AVLVSCAYQEIRAFLNRTYPDKGRNTSLSGFLGDLLVTCYSQFSRNRTFGNMIGQGYS
                     IKAAQLEMNMVAEGYHAALCIHEINKKHKVDMPIAEAMYKILHEFRSPAIVFKALSEQ
                     LK"
     gene            17124..18584
                     /locus_tag="PLM68_02165"
     CDS             17124..18584
                     /locus_tag="PLM68_02165"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOT54209.1"
                     /translation="MVNSYCSDKYQYTMAKTFVDKGLDRTTAVFNMFYRTAPDNNNWA
                     VVSGVNEVLEMISGFGTQNESFFRKFLPEDNYLDFCRMLTAVRFTGDVYAMEEGQIAF
                     PRQPLITVVAPLLEAQILETPMLCIMNHQMAVATKASRVTRSTTRPVSEFGSRRAHGP
                     WAALHGAKAAYTAGCVSTSNILATTEFDIASTGTMAHSYITAFGCSVRNEYDAFDAYI
                     KSHRGEGIILLLDTYDTLKSGIPNAIKAFKANGIDNSYHPLYGVRLDSGDLAYLSRKC
                     REMMDREGFTKCVIFASNALDEYLISDLERQNAAIDSYGVGDAIATSKHNPCFGNVYK
                     MVQMGDTPVLKLSEDKAKVINPGFQVTYRIYQDNESKADVTCLRGDSFACAIEEGREI
                     LLRDEIDQTRKTVYAEGSYTAEKLQHKVMENGIRIKPDTTQQEKRERYLYELSRMNPT
                     ERRIINPHYHKVDISDDLYDLKMKLIYTIKEELNHV"
     gene            18577..19143
                     /locus_tag="PLM68_02170"
     CDS             18577..19143
                     /locus_tag="PLM68_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT54210.1"
                     /translation="MYEETRTHIVVDMLNDFISGSMACQNAENAMTHSINYINMHPGD
                     KVVYVCDTHPENHCSFTGFGGQWPPHCVINTSGQEIHLSYYTRVNDPNMRPHLMRIFR
                     KGTSPKKEEYSGFNALRSDGKPLTDILTKQVIVSGIATEYCVLETVKDLVEAEYDVQV
                     LMEGLGYVNQKDHRAAIENMKSLGVKFV"
BASE COUNT         4955 a         4573 c         4867 g         4785 t
ORIGIN      
        1 aaagatctcg gaggtactta aggttggaca gcccgtactg gtacagattg taaaggaacc
       61 catctctacc aagggatcgc gtcttacttc cgacatatcc atagccggaa ggaatatggt
      121 gttattgccc ttttcggaaa agattaacct ctcttcaaaa ataagactga aagaagaacg
      181 aagcaggctg gaacgtcttg tacaaagtat cctgccgccc aattatggtc ttatcgtaag
      241 gacggctgcc gaaggaaaaa gagcggcagt cctggacacc gaattgaatt cgttgattca
      301 taaatgggaa gaatcctggt ctgtcatgcg aaagggaaaa atcccctctc gcttgttgac
      361 agaaaacagc cgtatcacta ccgtcatccg ggatctgctc aacgatacct ttaccagtat
      421 acatgtcaac gacagcatgg tatttgaaga ggtttgttcc tatatttcca ccatagcgcc
      481 tgaaaaggag aaaattgtaa aactatacaa gggaaaagag ctcatattcg atgcgtttga
      541 tataaaccgt cagattagga gttcctttgg cagggtggtt cccctcaggc agggcatcta
      601 tatcatagtt gagcatacgg aagccctgca tgtgatagat gtcaacagcg ggatccgctc
      661 aaagatggcc agtgggcagg aggaaaacgc ctttgaagtg aacatggttg tggccgagga
      721 gcttgcacgg caactcaggc tcagggacct tggcggtatc atagtggttg atttcattga
      781 tatggataac gcggaccacc gccttaagct ctacaaaaaa atgcaggaac tcatggcgtc
      841 tgacagggca aaacacagca tcctgcctct gacaaaattc ggcctcatgc aaattacccg
      901 tcagcgtgta cgtccggcca tggagatcaa caccagcgaa aaatgtccaa gttgcaacgg
      961 aacaggaaag attgcaccca gcctgttgtt tacagagaac attgaaaatc agctggcttt
     1021 ctttgcgaat gaaaagaaac tgaaaagcgt gatcctggaa gttcacccct ttgtagaagc
     1081 ttatcttgtc aagggattcg tatcaagagc caaaaaatgg tccagaaaat acggatgccg
     1141 tctcaatgtc aggatttccc ccgagctggc agtcacccag gccaggtggc tggatgataa
     1201 caatgaaaag atagagcttt aaagctgatt gagcgttttg gaaaaggtgt cctttagcgt
     1261 acagatcctg ttgaacaccg gtttttcctt tgtagtgtcg taatcagcaa caaaataccc
     1321 aagacgttca aactggaaac ggatacggga tccgtcctgt ccactcaccg cctggttggc
     1381 catggccact gcctgactgt ctatgaaagc ccgtattatc ttgagggatt caggattgag
     1441 gtactccctg taatccttat cctcaggaat ggatcccatg tagggttcca ggaataaccg
     1501 gtcaaaaaga cgcacttcgc ctgcgacagt atgctttgca gacaaccagt gtatggtccc
     1561 ctttacgctg cgccaggttc ctgtcccggg ttttgtgtcc ggatcaatgg tacaatgaag
     1621 ttccaggatg ttgccttcct ggtcccggat catatcgttg cacctgataa tgtatccgta
     1681 tttgaggcgt acttctccac cgggcctgag gcggaagtac ttcttgggag ggtcttccat
     1741 gaagtcgtcc cgttcgatat agatttcacg ggagaacggt ataagtcttt ctccggagcc
     1801 tggatcttca ggattgaggg gggctttcat gaactcttca gcaccttccg ggtaatttgt
     1861 aatgattgcc ttcaacgggt ccagaacagc cataaaacga ggggcggttt tattcaactc
     1921 ctcccttata caccattcca tgagtgaaat gtcaataacg ttatcccgct ttgcgacacc
     1981 tactttttct gaaaacaggc ggattcccga tggtgtatac cccctgcgcc tgagtccgca
     2041 aatggttggc atcctgggat cgtcccagcc gttgaccagt cctgtttgca ccagttccag
     2101 gagcttcctt ttactcataa cggtgtgggt aatgttgagc ctggcaaatt catactggtg
     2161 ggatggaaat atttcaagtt tttcaatgaa ccagtcgtat agcggtctgt gtacgtcaaa
     2221 ttctaacgtg cacaccgaat gagtgatctg ctcaatggaa tcacactgtc cgtgggcata
     2281 gtcatacatc gggtaaatgc accatgcatt gccggtacgg tgatgttcgg catgaatgat
     2341 cctgtacatc agggggtccc tcataagcat gttgggatgg ctcatgtcta ttttggcacg
     2401 aagcaccctg ctgccgtcgg gaaattcacc gttcctcatc ctggtgaaca aatttgtatt
     2461 ttcttcaatg ctccggttcc gccagggact gtccgtgccc ggggtggtaa tggtacccct
     2521 gttcaggcga atttcttcct gcgtctggtc gtctacgtaa gccaggccct tgttgatcag
     2581 gacaagcgcc cactggtaca gttgttcaaa ataatccgag gcatagaatt cgttagccca
     2641 ttgaaaacca agccattgta tatcatcttt tatagaatca acgtattccg tatcttcttt
     2701 tatgggattt gtatcgtcaa aacgcaggtt ggtctttcca ccaaactttc tggccaggcc
     2761 aaaattcagg caaatgctct ttgcatggcc tatgtgcagg tatccgttag gctccggggg
     2821 aaacctggta atgatggatt tatgctttcc ctgagccagg tcttcctgga tgatctcttc
     2881 aataaagttc agtgggcgtt cctctgaagt ctgtgtgttt tctgcgttca taagccggga
     2941 atatttgttg ggcaaaaata atcataataa tgtatatttg ctgaaaatat gatataatga
     3001 ttcgctcaat gaccggttac ggtaaagccg aagctttatt ttcaggaaag aaatttaccg
     3061 tcgagattcg ttccttaaat gggaaaactg cagatatcgg atttaaaaca tcccaaatac
     3121 ccagggaata tgaacatgac ttgcgtaaaa acctgattaa caggttgcaa agaggcaata
     3181 tcgatctttt catagcggta gaccaggaag tggctgatct ggacaatatt ttcaatgtag
     3241 aacttatcaa gcagtattat aaggaattgc aggccatttt cagggatatt gcccaggaag
     3301 tcagtccttc agaactggct gtttcccttc tcaggttgcc cgaggtcagg gaagtgcaga
     3361 aaaaagaagt ggatattgcc gaatggcaag tccttgaaca atgtatttat caggctgcgg
     3421 acaaactgga tgagttcaga tgccgtgaag gggaaatact gggacaggac ctgctatccc
     3481 atatagacaa cattgaaagc cacttggtca aggtgggaga acttgatccg gaacgtatag
     3541 tatcggtaag aaaacggctg gaagacaagt tggccggact gggtgaagat gcttccattg
     3601 ataaaaaccg cgtggagcag gagttgatct actatctcga aaaagcggat atcagcgaag
     3661 aacgcgtccg tttgagccag cattgcactt atttccgcca gactatcgtc caggaagaat
     3721 atgccggaag gaaactcaat ttcattgcac aggagatggg aagggagatt aatacgctgg
     3781 gctccaaggc agctcatgcc ggcatgcagc aatacgttgt tatgatgaag gatgaacttg
     3841 aaaaaataaa agaacaggta ctcaatgtcc tctaaaagta tatttttttt gatagcagcc
     3901 ttgctcctca cgaccgcttg cttaaagcag ccggaaaaca catataccta tgctgtggaa
     3961 ggagagataa ggaatattgt aaacttgccc gttgagggta taagggttat aatgaacaga
     4021 acatattccc ttaaaaacga agcagataca gcctatacgg atgcccaggg gaaataccac
     4081 gtatccctaa ccgtttctct gcgccagcgg atttttttgg tggactattc agatcccagg
     4141 cttaagtacc gggataccct gaggcgtttt acatttgagg atcagggaac actctacgat
     4201 accatgagcc tcaaatcacg gaaccaggtc gacccctttt agcgggggga aggaatttgt
     4261 tcaggatatt ttactgtaat cggcccttct ttttcccagg atttcaagct gtaaccgcaa
     4321 catcttgttt ttttccgagg caagaagcgg atcgtcatcc agaattttct gcgcttcact
     4381 tcttgcccat tcaaggattt gtccatcctt ggccaggctg gctatgtgaa ggtcaaaagc
     4441 cagtccgctc tgcgctgtgc cttccaggtc ccctggtccg cgcaaccgca tgtctgcctc
     4501 ggcaatctcg aagccattac tgctttcgca catcagtttc aagcgctgtc ttgcctcttt
     4561 gctcagggcg ttgcctgtca taaggatgca atatgatttt tcagcaccgc ggcccacgcg
     4621 tccgcgcaac tgatgaagct gtgataatcc aaaacgttct gcactttcaa taaccatcac
     4681 cgtggcattg ggaacatcca cacccacttc gataacagaa gtggcaatca atatctgcgc
     4741 atgtcctctg acaaacatgt tcatgtcata agccttgtct tcgggtttct gttgtccgtg
     4801 aaccacggca actgaataaa ggggggcggg gaaagccatg gttatgtcac gataccctgt
     4861 ttccaggttt tcataatcca ttttttcaga ttcacgtatc aggggataaa ctatatagac
     4921 ctgccttccc tccgtgatct gttttttcag gaaggaatag acggcgcccc tttttgaaga
     4981 gggatagtga atggtttgga cgggctgcct tccgggtggc agttcatcaa tcaccgatac
     5041 atccaggtca ccgtacagtg tcatggccag cgttctggga attggcgtgg ccgtcatgac
     5101 caggatatga ggcggtgagc cttctgtttt ttcccacagc cgggcacgct gctccacccc
     5161 aaagcggtgt tgttcgtcta tgacgacaaa gcccagcttt ttgaaggtca cggtatcctc
     5221 tatcagggcg tgtgttccga taagcaggtg caaggacccg tcctgcaaag attcaaggag
     5281 tgccttgcgt tcagaagctt tggtattgcc tgtaagcaac cccactttga tgttctgtcc
     5341 cttcagaaag cggcttacgg tattatagtg ctggttggcc aggatctctg taggggccat
     5401 catgcaagcc tggtagccgt tatctgttgc aatcaatgag ctgagcagcg caacaagggt
     5461 ttttccgctg cccacgtctc cctgcagcag gcggttcatc tgtcgtccac tccccagatc
     5521 ggcgcggatt tctttgatga cccgtttctg tgccgaggtc aactcaaagg gcaacctttt
     5581 gtaacaattg ttgaaagact cgcccactgt ggaaaaccgc agcccgtcct cggccctcag
     5641 acggactgcc cgttgcttaa gcaggccgag ttgaatgaaa aacaattcct cgaatttcaa
     5701 ccttgtttgt gcagcctgga ggtttttcag gttcttcggg aaatggatct cttccagtgc
     5761 catggccagg ggcatcagtt tcagttgctt gagcatgagt ggtgttagcg gctccactac
     5821 ctgtccttca acacggttaa gggcctgggc aatgagacgg gaaaagactt tttgggtaaa
     5881 acccttgtca cggagtgatt cggtggaaga ataaacggct tgaagtcctg aacttcccct
     5941 caggctctgg tcatcgggga tgtccatatc ggggtgtacc aggttcatcc tgccattgaa
     6001 caaggtgggt tttccaaaaa caatatattc cgtgttcacc ttgattttat ccttgatcca
     6061 gcggattccc ttaaaaaaaa cgaggtcaac ggttccggtg ggatctgcaa agagaaccac
     6121 cagccgctgt ttgcgggaac ctgccccgac gattttcata tccctgacct cgccccggat
     6181 ctggatagct gccagatcgg gatcgatttc actgattgaa tagaaacggg tcctgtcaat
     6241 gtaacggaaa ggataatgac ggatcagatc gccaaatgtt tttatattca aacctttttc
     6301 cagttccctg gcacgcccgg gtcctactcc ctggagaaat tgtatgttag tgtcccatac
     6361 agaagtcata gccatgcaaa tataacgata tcttgcgtat atttgcgata tgagcatact
     6421 gggaaacata ctctggctat tgtttggcgg tatcatgatc gcgctggaat acatggttgc
     6481 aggcgttctc atgtgtatca ccatcatcgg gataccattc ggcattcaaa gtttcaaact
     6541 ggcagctttt gcactctggc cttttgtgaa aacaacaagg acggtagagt ccgggaccgg
     6601 ttgcctgagt acggtgttga atatattgtg gatctttctc gggggtatct ggattgcact
     6661 cagccatctc ttctgggggg tggtgttgtg catcactatc attggaatac ccttcgggaa
     6721 acaacacttc aagatggtca ccctggcatt tacccctttc gggagggaaa tcatctacaa
     6781 aaattaactt atcatgcata aaaagataat agtcgggctc acccagggtg atacaaatgg
     6841 gataggatac gaagtactga tcaaagccct ggccgattcc cggatcaatg aactgtgcac
     6901 tcccgtgatc tatggttcgt cacgcctgct ggggtattac aagaaattgt gcgcaggggt
     6961 ggaaaatttc aatgttcata tcacgcaagc tgcttctgaa cttcatttca aaatggtgaa
     7021 cctgattccc tgtgtgtcgg atcagttcat agccgaaccg ggaaaaatga caccggaagg
     7081 cgcacaggca gccatgcaat cactcggacg cgccgtagag gatctgcgcg gcgggaaaat
     7141 ccaggtgctg gtgacagctc cctttaacaa gcagggcgtg aacagggagg gatttgtttt
     7201 tcccggccat acagagtatc tggccaatgc ctttggtgtt tccaatccgc tgatgatgct
     7261 ctgcagccat ggactgagga ttggtgtggt cacaggtcat atcccgttga gccggatacc
     7321 tgcggaactc acaaaagata agatcgtcaa gaagataaag ctgatggata gtacccttaa
     7381 gaaagacttt acgatcatgc agcccaggat agccgttctg ggactcaatc cgcatgcggg
     7441 agacggggga ctactgggaa cagaagaaga acaggtcata ttaccggcca tagaagaggt
     7501 caatcgatca gggattctgg cattcggtcc ttatcctgcc gatggtttct ttgccgacgc
     7561 agggtacagg aaatttgatg ccattcttgc catgtatcac gaccagggtc tggttccttt
     7621 caaggctttg tcattccgcc agggtgtgaa ttttacagca ggattgcctg tgatcaggac
     7681 atctccggat cacgggacag cttaccgcat tgccggagag gacaaggcat ctgcagaatc
     7741 catgcttgct gcaatcttta tggcttgtga tatctacagg acgcggcgta ggaatcaggc
     7801 cataacggaa ggattgcatg aaggttaata tctataccga cggatctgcc ctgggaaatc
     7861 ccggacccgg aggatacggg gtgatcctgc aaagcggcag acactataag gaaatttcag
     7921 ccggataccg ccttaccacc aataacagga tggagttgat ggccgtcatt gccggcctgg
     7981 aagcactcaa aaaacacgga acagatgtct gcatctattc agactcggcc tatgtcgtaa
     8041 gggctgttga agaaggatgg cttcaaaact ggaaagccag ggatttttgc aggaaaaaga
     8101 atgtggacct ctggaaacgt tacctggatg ttgcatcaaa acacaaagtg cggtttgtct
     8161 gggtcaaagg ccacgccggt catccacaga atgaaaaatg cgacagcctg gcagtggctg
     8221 ctgcctctgc tcccgtagaa accttgctgg aagaccaggg attcattgac gggctttcat
     8281 tatcgcctgc agggcatctg gatgaactac ctggttggag gaacgactga tcaggacaaa
     8341 atccggggcc ccgttatccc agtaaaaagg caccatccca tgccgtatgg cagcttctgt
     8401 aacatactcc agataatggg aacggcattt tttatgttct ttgagttcgt ccccggtgag
     8461 ctgaatcctg tataatgccc cgtattctcc caggatcaca gggaatcctt tatcggtgaa
     8521 ttttttcatt ttgccgaagg ttgactccac ccagtcttcc tggccccagg atgttacggg
     8581 gccggttgct acggaacccc agcggaaatc atacggttca tcctccttta atgcaaattc
     8641 atacggatcg tagtaatgca cttccaccat gagcctgttg ggggtgggat cctgtggtat
     8701 gatgagtgat ctgtaagcca ggtcaatatt ggtaaggtaa ctctgtatga ccaggtggcg
     8761 cttgaggttg cgtcctcccg tttctcttac ggcatctaca aaagtctggt tgaaagaatt
     8821 ctgcacagac agatattcag ccggcgggct ggaagaccag tcaccttcca tatggacttc
     8881 gttggagccg gcaaaaagga gccggtcatc ataatccttg aaatatgtgg caatctgggt
     8941 ccagacggcg tggagctttg cgttgacctg ttgccggctg ctttccctgg gatcgttcat
     9001 ccagccgttg tcccagtgga tgtttataat ggcaaacata tcattgacca gcacataatt
     9061 gacaacctgt cccacacgct gcatccaggc attgtctatc ttgtgtgtgc cctggtctgt
     9121 aatgtgcaca gaccaggaaa cagggatcct caccgaggtg aatcccgctt ctttgaccgc
     9181 cctgatcagg tctgatgtca cctttggatt accccacgat gtttcaccac ccgttgcttc
     9241 cagggaattc cccaggttcc atcctatgcc catgagcttt gataattcca tggaagtaat
     9301 gtcgcgcatg gcgggatctt cgccgttatt ttcgctttta cacccggaaa tgagcaggca
     9361 aaacagaaga aggattgtga atgttttcat aaagacctat ctttgaaaag gcaatactaa
     9421 tgataatcaa agatatgaaa aaatgtttct ttatcctgat cacgggactt ttattgattt
     9481 cgtgtacacg taccttgctg gttgacagta acagcgtgac ggtcaccgtg tctgacaaat
     9541 caaaagtcag gatccaacct gtcagcccgc agatcatccg ggtgtccgct gttcctgcac
     9601 gggaaaaatt ctctgcccga cccagcctga tgcgggttcc gggagagaca cccctggtcc
     9661 cgttcgactg cctggaggaa ggacagcggg ttatagtaaa gactgaagcc cttcaagcta
     9721 gtgtgaacat acatacgggt gaagtggttt tcatggatcc ggagggaaac gtgatcctgc
     9781 aggaaagcca gggtggggga aaatcattca ctcccattga agtggaagga gacaagggat
     9841 attccatccg gcaggttttc gaatcacccg ccgatgaagc cttttacggt ctgggccagc
     9901 atcagtctga cgaattcaac tacaagggta aaaacgagac cttgtaccaa tacaacacca
     9961 aggtttccgt acccttcatc ctatcaagca atcattatgg attgctgtgg gacaacaact
    10021 ctttgtcacg attcgggaat ccggaagact acaagcaact ggacgcgctg gacctcaggg
    10081 atattgacgg acaggaaggg gctctttctg ctgtttatgc cagccgagac ggaaaaacgg
    10141 aatacctgcg cagaagggaa tccagggtag attacacgga ccttgacaag gtgaaaaact
    10201 tcccggaagg gatccccttc cataatgcac agattatctg ggagggaacg ataggggcaa
    10261 aagaaagcgg gatacaccac tttttgctct actatgcagg gtatactaag gtttttttta
    10321 acggagagga agtggtaggg gagcgctggc ggacagcctg gaatccgaat agttacaaat
    10381 tcacagcgga aatggaagcc ggtattgaat accccctgcg catagagtgg gtacccgacg
    10441 gaggggtttc ctacctgggc ctttcgctct atacgccggt tgatcccctg gaacaggaaa
    10501 agcaatcctg gtggtcagaa atggccgata tgattgatta ttattttatc tatggacagg
    10561 atagcgacca gatcataagc gggtaccgcc tgctgaccgg caaagctcag atcatgccca
    10621 aatgggccat ggggtactgg cagagccggg aacgctacaa aacctcagat gagttactgg
    10681 gggccctgaa tgaataccga aaacgcagga tccccattga caacatggtg ctggattggt
    10741 tctactggcc tgtagattcc tggggaagcc atgaatttga caaagaaagg ttccccgatc
    10801 ccaaagccat ggttgacagt gtccacgcac agaacnnnnn nnnnnaacgc ccggatcatg
    10861 atctcggtat ggcccaaatt ttatcatacc acagaacatt acaaggaata tgaggataat
    10921 gggtggatgt acacccgtgc ggtggaggat agtatccgcg attggatatg gccggggtat
    10981 atcggttcat tttacgatgc ttttgccgaa ggagcccgca ggcttttctg gcaacaaatg
    11041 gaagatcacc tttatcccct gggatttgat gcctggtgga tggatgcctc ggagccggat
    11101 atcctgtcca acgccagtct ggaatatcgt aaagcactct ctacgcctac cgctctgggc
    11161 ccctcggcca ggtatctgaa tgcctattcc ctggtaaatg cccaggccat atatgaaggt
    11221 caacggggtt cagacccaga caggcgcgta ttcctgctga ccagaagcgg attcccggga
    11281 ttgcagcgtt attccactgc aacatggagc ggggatattg gaacatgctg ggaggatatg
    11341 aaagcccaga tttctgccgg catcaatttc agtatttcgg ggatacctta ctggaccatg
    11401 gacgtcgggg gcttctgtgt acagggacgt tttgaaaggg cccgggaagg atcacccgat
    11461 atggaagaat ggcgcgagct gaatgcccgc tggtaccagt ttggagcttt ttgtccgctt
    11521 ttccgcagcc acggtcagta tccttaccgc gaaatcttca acattgcccc tgaaaaccat
    11581 cctgtttaca aaaccatggt ccaatacaac aaactgcgtt accgtttaat gccttacata
    11641 tacacgctgg caggaaagac ctggtttgaa gactatacca tcatgcgggg tttgattatg
    11701 gattttgctg aagatgagaa tgttattaat atatccgatc agtatatgtt tggaaacgcc
    11761 ctgatggtat gtcctgttta tgaatacaaa gccaggaaac gcgatgttta ccttccccgc
    11821 cagaatgact ggtatgattt ccataccggg gaatacctgc ggggaggcaa cttcctgacg
    11881 gccgatgctc ccctggataa aatgcccgta tatgtgaaag caggttccat tttaccgaca
    11941 ggtccggaaa ttcagtatac ctgcgaaaaa cccggagcgc ccatcacctt gtgtgtatac
    12001 acaggggccg acgcccggtt caccctgtac gaagatgagg ggataaatta taactacgag
    12061 aaaggtttct tctccaacat tccttttcac tacgatcagg aaacagggat ctttaccata
    12121 ggggccagga aaggatcctt tgaaggtatg ccggagagca gggagttcca tatcaggaaa
    12181 atcacccccc acaagccggg aacgcctttt gccgggggag aatcgctgac ggttgtcagt
    12241 tatgacggta aagaacaaat catacagctt taagtcttat gaaacgcttg tatatcatag
    12301 cagctgcctt actgatcctg ggctcctgcc acagggattc gctgcggaaa gtcaccgatt
    12361 tcaacgacgg ctgggaattc gcccggaccg gggctataga tgactcattg atctgggagt
    12421 ctgtagacct tcctcacgac tggagcattg agggaccttt tgacaagaac catcccgcga
    12481 ctccgggagg aggcgcttta cccggaggca agggtatata cagaaaagtg ttcaccctgg
    12541 aaccagggac ggaagggaag cgtgtattcc tggaatttga cggtatatac cgcgacagcc
    12601 gcgtctttgt gaacggtcac ctggcaggac accgtccctg cgggtacgct tctttcagtt
    12661 atgaggtcac tccgctgttg aatcctccgg gagaggagaa cagggttgag gtcaccgttg
    12721 ataattccct gcaaccgaac tcaagatggt acacagggtc cgggatctac aggaatgtcc
    12781 gcctggtggt gactccggag gttcacatcc cttattcggg aacattcgtc accacacccc
    12841 aggtttcgac cgatctggca caggtccggg tcaggacaac cattgtccgg cctttcgcag
    12901 cgaccggaga tttctacctg cttactaaga ttgtggaccc ttccggactc actgtggcta
    12961 aagaatgcaa ttacgcggaa ccttcaccgg gaggtgaaat catccttgaa cagacactgg
    13021 aagtaaagaa accggctctc tgggatgttg agaatcccag cctctatacc gtggaaagcc
    13081 agttgctcag gggaggtgct gtcatagaca attacagtac cacgtttggt ataagaacat
    13141 tcaggtttac agccgattcc ggtttttacc tgaacgaccg tccctttaag ataaagggag
    13201 tatgcatgca ccacgacctg ggagccctgg gagccgctgt gaaccgcagg gcgctggagc
    13261 ggcagcttga gatgctggca cagatgggat gtaacgccat tcgtacgtct cacaatccgc
    13321 cggctcctga attgctggac ctttgcgata gtatgggttt cctggtcatg aacgaagctt
    13381 ttgatgtctg gcgcaagaac aagacagctt acgattatgc catgttcttt gaagaatggt
    13441 atgagaagga cctgagggat ttcatcctgc gcgaccgtaa ccatccttgt gtggtcatgt
    13501 ggagcatagg gaatgaagtg ttggaacaat ggaacaatcc tgtggccgat acgctggatc
    13561 tgcagcaggc gaacctgctc ctgaacttta tggcaggaga tgacacccag gtccagggag
    13621 accttccctt tgatgcactg ctgaccagga agctggccag gatcgtaaaa gagctggacc
    13681 caacacggcc tgttacagca ggttgtaatg agcccgcacc ctacaataat ttgttcaggg
    13741 ccggggagct ggatatcata ggctataatt accacgaaag cgattacgcc aaagtaccgg
    13801 tgaattttcc cggaaaaccg ttcatagctg ctgaaaccac ctcttctctc catacacgtg
    13861 gtttttacca gatgccttcc gactcggtaa ggaaagaacc aaaacaatgg tggatgacct
    13921 atgatacccc gcaccacatg tgttccgcct atgacaacat gtgtgcaccc tggggaaaca
    13981 cccatgaaag cgcctggatg caggtccgcg acaacaattt catctccggc atgttcatct
    14041 ggacaggatt tgattacctg ggagaaccca ctccctactg gtggccggcc aggagttcac
    14101 atttcgggat aattgacctg gcgggctttc ccaaagacat ttattacatg tatcagagcg
    14161 aatggaccga taccccggta ttgcatgtgt tcccccactg gaactggaat ccgggagaga
    14221 tggtggatat atgggcttat tacaaccagg ctgattccgt ggagttgttc ctcaacgaaa
    14281 ggtcgcttgg tattagcagg aaggattctc taaaactaca tgcctgctgg cgcgttccct
    14341 ttgaacctgg aacgattact gcagtctctt acagtgaagg caaggaattc ttacgccgga
    14401 gtatatccac ggcaggagag ccggccagcc ttaaactgac ctctgacagg gttgtcctgg
    14461 agacaggggg cgaagacctg gcttttgtaa cggtagaaat tctggatgag cagggtaatc
    14521 ctgtgcctta tgcagacaat atggttactt tccgcctgga gggtcccgcc accatagcgg
    14581 ccgtggacaa cggcagtccc ataagccacg aaccgttcag ggccgattac aggaaagcct
    14641 ttcacggtaa gtgccttgta gtgatccgtt cgggaaaaac agcagggaaa atcaggctga
    14701 ctgccctttc agagtcgttt gcgcaggaga gcaccctgga cctcgaaacc cgacttgtcc
    14761 aataaggcga acatattttt tttgtaagct tccacgcctt cctggtcaaa tggattcacg
    14821 cccaggatat acccgcttat gccgcaggct ttctggaaga aatagagaag ggctcccagg
    14881 ttgtatgcat tcagggaaga catgtcaatg cgcaggttgg gcacaccgcc atctgtatgg
    14941 gccagccgcg tccccagctc tgccattttg ttgcaatgat ctatacgttt tcctgccagg
    15001 aagttgagcc cgtcgttatc cccttcatcg gcgggtatgg ccagtgaacg gttgggtgcg
    15061 gcaatgctta taaccgtttc aaaaagatgg cgttccccat cctggatata ctgtcccatg
    15121 gaatgcaaat cggtcgtgaa atcaacaccg gcagggaaaa tgcctttatg ctcttttcct
    15181 tcgctttcgc caaaaagctg tttccaccac tcggtcacat aatgaagttt gggatggtaa
    15241 ttgaccagca actcgatctt tttgcctgtg ctgtataagg cattacgaag agctgcatac
    15301 ctcactgcgg gattatttcc tgttttcagg gctgtgatct tttccatatc ccgggcgccc
    15361 tccatcagct cttgaatatc gatacctgcc agggctatgg gaagcaggcc aaccggggta
    15421 aacacggaaa aacgtcctcc tacgtcggaa gggatgataa aacttcggta ctcttcgctg
    15481 ttgaccatct tgcgcaatgc accgtgggat ttatctgtta tggctacaat gcgccgcctg
    15541 gtttcttcct tcccgtaacg ggacaggagg tgttgcttga ccaaccggaa agcaatggcc
    15601 ggttcggtgg ttgtcccgga tttggagatc acaacgcagg ctgacgactt gacagccatt
    15661 agatcttcca gctcggccag gtaatcttcc ccgatattct gcccggcaaa cacaatatat
    15721 ggcgttgaag acggcatcag ggcggaaaaa gagtgggaga gggcttcaat gacggccctg
    15781 gcacccaggt aggatcctcc tatgccaata accacaacca gttcgatatc tttccaatcc
    15841 aggcatatgt ccttaatctg ctcatagaca gtcggtgcag tctgcgaggg aaggcgaagc
    15901 catcccagga aatcggatcc ctttcctttg cctgtctcaa ggaggtccag ggcccgcagt
    15961 gctttctcct gccagagggg ataggtatca ccgggaatga atccaatggc agagttttct
    16021 ctaatggtta tgatttcttt catgggttat ttcaactgtt cgctaagtgc cttgaaaacg
    16081 attgcagggg agcggaattc atgcaggatt ttgtacatgg cttctgctat gggcatatcc
    16141 actttatgct ttttattgat ctcatggatg cacaaggcgg catgataacc ttctgccacc
    16201 atgttcattt ccagttgtgc ggccttgatg gagtatccct ggcctatcat gttcccgaag
    16261 gtccggttgc ggctgaattg agagtaacag gtaaccaaca ggtctcccag aaatccggag
    16321 agtgacgtgt tcctgccttt atccggatag gtacggttca gaaaagccct gatttcctgg
    16381 taagcgcatg aaaccagcac agcctggaag ttgtccccgt atcccaggga gcggctcatt
    16441 ccggatgcta tggcataaat gtttttcagg acgacggagt attcaaggcc gtagatatct
    16501 gtgccaatgc tggtgcggat gtagggacag cggaaaactc cggcaagctt tttggcggtg
    16561 ctttcctttt tacaggtaac actcaggtag gagagccgtt ccatagccac ctcttccgca
    16621 tggacaggac ctgctatcac acccatattg tcgtaaggaa ttttgtggat gcggttgaag
    16681 tactcggtga cagagatgtt acgctcaggt attaaccctt tgatggcagt gactatgaac
    16741 ttttcatcca gcgggactgt aaggggaatc aactcgctga tgaaataagt agatggaatg
    16801 cagaatatca gaacatctcc ctgggaaatg accttgttga tatcacaact cataagcagc
    16861 cgggaagtat ccagttccac agcctgcagg taatgtggat tatgtttgtc ctgatctatg
    16921 aaattgatca tattctggtc cctgacaaac caaaggactt catcctgaga gtcgagcaga
    16981 atcttgacca gggccgttgc ccagcttccc gaaccaatta ttgcatattt agtgtttttc
    17041 ttaaaaaggc ccttcatagt tttccggttt aagtcaacaa agataagctt aatttgtcta
    17101 tctttacgtt ttaaaaaatc acgatggtca attcctattg ttccgacaaa taccagtata
    17161 ccatggccaa aacttttgta gataaaggtt tggacagaac caccgccgtt tttaacatgt
    17221 tttaccgtac agctccggac aacaacaact gggctgtggt aagcggtgta aacgaagtgc
    17281 tggagatgat ctcgggattt ggaacacaaa acgaatcttt tttcagaaaa ttcctacctg
    17341 aagataacta tctggatttc tgcaggatgt tgacagccgt acgctttacc ggggatgtct
    17401 atgccatgga agaaggtcag atagcttttc cccgtcagcc ccttatcacg gtggttgccc
    17461 cattgctcga ggcccagatt ctggaaaccc ccatgctgtg cataatgaac caccagatgg
    17521 cggttgccac caaagcatcc cgggtcactc gcagtacgac ccggcccgtg agtgaattcg
    17581 gctcaaggag ggcacatggt ccctgggctg cccttcacgg ggcaaaggct gcgtatactg
    17641 ccggttgtgt gagcacctca aatattctgg caacaacgga attcgacatt gccagcactg
    17701 gaaccatggc ccatagctac attacggctt tcggctgcag tgtcaggaat gaatatgatg
    17761 cttttgatgc atatatcaaa agccacaggg gagaagggat cattctgctt ctggacacgt
    17821 atgacacact caagagcggt atacccaatg ccataaaggc ttttaaagcc aatggcatag
    17881 acaattccta ccatccttta tacggagtac gcctggacag cggggatttg gcctaccttt
    17941 cgcgtaaatg tcgtgaaatg atggacaggg aaggattcac caaatgcgtg atctttgcct
    18001 ccaatgcact ggacgaatac ctgatctcgg accttgagcg tcaaaatgca gccatcgaca
    18061 gttatggtgt gggagatgcc atagccacca gcaagcacaa tccctgcttc gggaatgttt
    18121 acaaaatggt acagatggga gatacccctg tcctgaaact ttccgaagat aaagccaagg
    18181 ttatcaatcc gggtttccag gtcacatacc ggatatacca ggataacgaa agcaaggccg
    18241 atgtcacctg cctgcgcgga gacagctttg cctgtgctat tgaagaaggc agggaaatcc
    18301 tgctcaggga cgaaatcgac cagaccagga aaacagtgta cgcggaagga tcctatacgg
    18361 cagaaaaatt gcagcacaag gtgatggaga acggaataag gatcaaacct gatacaacac
    18421 agcaagaaaa acgcgaaaga tacctttatg agttgtcccg tatgaatccc acggagcgca
    18481 ggataatcaa tccccattat cacaaggtgg acatatcgga tgacctgtac gatttgaaga
    18541 tgaaactgat ctatactata aaagaggaac tgaaccatgt atgaagaaac cagaacccac
    18601 atagtggtgg atatgctgaa tgatttcata tccgggtcca tggcttgtca gaatgcggag
    18661 aatgccatga cccacagcat caactacatt aatatgcatc caggggataa ggtcgtctac
    18721 gtttgcgata cgcatcctga gaatcactgc tcctttacgg gattcggagg tcagtggccc
    18781 ccgcactgtg ttatcaatac ctcgggccag gaaatccacc tgtcctatta cacaagggtg
    18841 aacgatccca acatgaggcc gcatctgatg cgcatattcc gcaaaggtac ttctcccaaa
    18901 aaagaagaat actcaggatt caatgcctta cgctcagacg gaaaacccct gacggacata
    18961 ctgacaaaac aggttattgt gagcggtatt gccaccgaat attgtgttct tgagacggtc
    19021 aaggacctgg tggaagccga atacgatgtt caggtactta tggaaggact cggttatgtg
    19081 aaccaaaagg accaccgggc cgccattgag aacatgaaaa gtcttggcgt caagtttgtc
    19141 tgataatcct ggaaagatat atgcatgcca taggtgtaga tctcggtggt
//
LOCUS       DAOSOF010000020        19072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2891_length_19072_cov_4.037913, whole genome shotgun sequence.
ACCESSION   DAOSOF010000020 DAOSOF010000000
VERSION     DAOSOF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2891_length_19072_cov_4.037913"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLM68_02175"
     CDS             complement(<1..286)
                     /locus_tag="PLM68_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT54211.1"
                     /translation="MKRSAIILCALFLLFASCRKEPTPGSDPDNGASFSRVKETPRAW
                     DGNKRADITYQLLVYSFADKDGDGWGDFQGLKDKLEYIDQLGATAIWLSPV"
     gene            complement(291..2843)
                     /locus_tag="PLM68_02180"
     CDS             complement(291..2843)
                     /locus_tag="PLM68_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HOT54212.1"
                     /translation="MKILKYLFAAAIVSLSAVSCEVISEDAFSTAPVPPEMYAHSDIL
                     MTSNTMDEFVNFSWKPARFLGEGLTYDLYGKFGESTTKLASTSELYYRVPKTEFKTAL
                     YSAFPALPENDTFTMSFFVSVTNQDESFESDPVTIDIYAYGDAVSPETSALVESIVLD
                     VTDPEGVLELLTWEPARLGYNEEITYSVSLQYGETAAIVLLASDLKETSFSITVDALN
                     EAAVAAGAPEAAAADLDFTVRAFSATYPDGVISNTVTINVTTYIATYPEYMYVPGAYQ
                     GWNPATAPKIKHSSVTKGLYEGMIDLSVEGGGDVGFKFSPVPAWENDFAIANIEKTTF
                     GNGYTALTGKGTTGGDITAPSGMYNILLNKKLNTLYMIQVETLSLIGSAPVASNGWVN
                     DIDMTYDPVAHTFSAITNMQEGEFKIRVNHDWTHSAGGTVDNVSFGKGDNISFTRTPG
                     EYKVVLNVGVNPYTIKFINTSFPEKMYVPGNHQGWNPAAAPVLNGDNEGHYEGYVTLT
                     DIFKFTSAPDWNHTNYGGSMESLDTDPSAGNLQVPAPGYYYLKVDLTTMSATATLIDR
                     VGVIGSFTGWGEDVPMTYDPNTDLWTATNLPFPAGTEYKFRMNNDWAINLGGDPNDLK
                     QDGANLKADAGMYTVILSLATTPYHMTITRTGDLVSEYGTEVVVAGDYSGHSWSATDD
                     PKLQGSGDGTYKGAITMYNMQYGFKIVESGNWIAGQLVADSQYEFTLYTGDNMMLPNG
                     TYFWSVDLPDTKAVATPVTVVGLIGSFEGSAWGTDVAMDFDQETLTYSATLNLAAGNE
                     FKIRFNNNWDLNLGGDITNLTHNGANIMVQEAGTYRIVLNMANTSSLTMTKQ"
     gene            complement(2864..3946)
                     /locus_tag="PLM68_02185"
     CDS             complement(2864..3946)
                     /locus_tag="PLM68_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HOT54213.1"
                     /translation="MKHTKYITMLVASVLVLVSCDIQIEKAFLNPVSGFKAPVLLSQG
                     SVIINEDNNKTESVTFNWSSASFGPAVQVEYSLYATANSQTALLGSSFTNSLSVSKSD
                     LNGVVANDLKVGVNATASISAYLVAKVYGTNVESVTSNSIAFNVTTFAAALRSLYLPG
                     NYQGWKIEQAPEFWETDGGTNVYKIMVDLEDGSDQYSYFKVTVARNWSDLNWGYNFLT
                     PSWYCPEQSDSNLSVDLTEGNIYVLTVKRGKMTIDKQLVKKVGIIGAFDESGWGTELD
                     MVYDEVNNLWVSPAITFSGDKNFLIRLNESWDDKYGTNNEASTGVEGGLKLVKGGGDI
                     NVPSTGTYIMKLHGNRTPFVLVMEKQ"
     gene            complement(3977..5647)
                     /locus_tag="PLM68_02190"
     CDS             complement(3977..5647)
                     /locus_tag="PLM68_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54214.1"
                     /translation="MKKILKNSFLLALAFIFLLTSCINDLDTLPLNETDATSETAYAE
                     PSSYLNGLAYLYGYWALVSQNDMGSPDIAVADAGQSELSRMYMVLNELSTDSFKIIWG
                     DNYIVPIQYHRWTSADNEAIIAVYTRCMKGITLVNEYLIQTADEKLNDRGHSAEIATV
                     HQYRAEARFNRALYYYLLMDLFGNPPFALEENIGGELPKQIGREALYTWLEEELLDLA
                     NSTDMPAKGAVPYPRATKGSVWALLARMYLNAEVYTGSPQWQKAKDAAGTVIGMGYTL
                     HPNYQELFMQDNTTNGATQEFIYAVVYDKDNTQSWGGTTHLVSATLSSDANNAIAKNL
                     GLSGLLNPETWNGYHVADDYVQRFELEGVQWGATSGFGYDREKSDKRAFFYNIGSSKD
                     FDNTTTDTGWRCWKFTGLYSDGHTVSDAESNYKFSSIDYPIFRLAEMYLIYAEANARM
                     SGGVVNDPTAKGYINQLRERAGVASKTPETMDLDWLLDERARELMWEGHRRTDLIRYG
                     YFTSMQYPWTLKGGVLNGKVSLPEHRKVYPIILSDLNANPNLVQNTGY"
     gene            complement(5660..8647)
                     /locus_tag="PLM68_02195"
     CDS             complement(5660..8647)
                     /locus_tag="PLM68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54215.1"
                     /translation="MKRLMTFLLAVTLFSVTSVYAQYEVRGTVVDVNGEAVIAATVLQ
                     QGTSNGVTTDLDGQFTLRVPSPETLLEISFLGYQTVVVAAGNATRIVLHEDSELLEEL
                     VIIGYGTVKKSDMTGSVVAIRAEEVNRGAVVSPDQMLVGKVSGLRITPATGQPGASAT
                     IRIRGAASLNASNDPLIVIDGVPVTGDGGAGMGNPLASVNPNDIESYTVLKDASATAI
                     YGSRASNGVIIITTKKGTGKGFNVSYNSSYSVKQNASSIQMMTADEYRDFMATTYPNN
                     QIIPTLLGTENTDWQKEIYRLSFNTDQNVSLYGSTRHMPYRLSLGYNLDQATLKVGDN
                     QRANVDISLTPKFFKEHLTVNVNVKGIYQKTNWANTGAIGNAMAFDPTKPVYFTKTDG
                     SIDTSKANGYWNWFNADGSANTMASTNPLSTIYDYTNFNQTLRSLGNVQLTYKVHGLE
                     DLTANVNAGYDIADTWGERHNALGSISAMRSSNDLFVTYENYNLNTLLETYLNYNHDF
                     RNSNLDVMAGYSWQHNYVQYDEEQFKNTEPDGPGAHYYTAPTNAKEYYLLSFFGRINY
                     SIASKYLFTVTLRNDASSRFSKANRWGLFPSAAFAWNIGQENFLKSSRAVSALKLRLG
                     WGKTGQQDIGSDYYPYLARYLSSSAIEMRYFMGDGFYTTLAPLAYNANLKWETTETYN
                     VGLDFGFASDRITGSAEAYYRNTYDLLNVISTPLGSNFGNSIISNIGNMVNKGLEFTL
                     NFIPVQTADTHWSIGGNITIQDTKITKLTTLKTEGYLGVTTGSGMGGTGGYSSLHREG
                     FTPYTFYMFQQLYDNEGNPVQNGLIDRDGDGEITDADRYVTGFSPTPDAFFGINTQFR
                     YKNWDLGLNGHGSLGNYIINGVAMGYSTSYSDDWNKGYLNNVSKQYLVQGWTAPSENN
                     QKYSDMFIENASFFRIDDINLGYTFDNLKFAKSIRIAASVQNVYVFTKYRGLDPEIVS
                     SDGVDFNIIPRPRLYTLRLNINF"
     gene            complement(8674..10026)
                     /locus_tag="PLM68_02200"
     CDS             complement(8674..10026)
                     /locus_tag="PLM68_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54216.1"
                     /translation="MTQKKMTFWNIWNMTFGFLGIQFGLALQNANVSRILQSFGADVS
                     HLSLFWIAAPLTGMIVQPIIGHYSDQTWTRLGRRRPFFLTGAILAALALVLMPNAPML
                     APIISPMLIGAGILMIMDASINVAMEPFRALVADLLPSEQRTMGFSVQTSLIGVGAVL
                     GAILPYLLTNWFGMANTPDVEAGHLVANNVKYAFYIGAAVLFGSVLWTVISTREYPPQ
                     EGVRPEKQPFWQIGKDFLSIPKTMKQLGVVQFFSWFALFSMWVYMVPAVADHLYGTTD
                     TTSRAYADAGDWVGILQGVYNGVAAIFAFLLPMISKKISRKGTHALCLLMGAIGFLSF
                     YLIKDPRMLVFPMIGVGIAWAGILAMPYSILAGALPAHKMGVYMGIFNFFITIPQIIN
                     GLVGGVMVKYLYGGKTIFAMVFSGLCLLIAAVSVFFVEDRDDPVQLAFRKNRKIGLSN
                     "
     gene            complement(10145..10897)
                     /locus_tag="PLM68_02205"
     CDS             complement(10145..10897)
                     /locus_tag="PLM68_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT54217.1"
                     /translation="MKVLILEDEIPAQMQLKRLFSVYYPDAIIAAVLDSVDKAAKWLA
                     LNSADLILMDVELSDGVCFELFNRISPMPPVIIVTAYEHYALAALKKNAVDYLLKPVG
                     DSEFVEAVEKCIKSVERPQDLHNLGKLINQYQDFKQRFVIKVGDQIIVISTKDIAYFF
                     SEDKSTYIMTREGKHYLSDASLETIEEQLNPRDFFKLSRNCIASISAINSISKFFNSR
                     LRIELLPPYKQSILVSRQRVPVFMRWIEGESD"
     gene            complement(10901..11941)
                     /locus_tag="PLM68_02210"
     CDS             complement(10901..11941)
                     /locus_tag="PLM68_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT54218.1"
                     /translation="MAPVTKRTTIIEVLLLAFFVLAFRLAFEGAIPFFQNNFNLNFNE
                     TLSRLLGNYPLALLMIILDFLLVWMLVRHIDYGAKPMLRLLLELSGLTVIALFSAILV
                     RIMSARDGPAEGSFFNYNFWYTALAAFFFNMIFIAGMDIYAYYGRVQKKALDIEISQK
                     NKARYQYQQLKHQLNPHFLFNSLNSLDYLIRTDAGKASEYVRKLASLYRHFLNIGEQE
                     SIMLKEELEFVELYVDLLSQRFDNSLKVTFKISEEYLNRLVIPCSLQVLMENATKHNI
                     VSKDSPLEILITTEDDHLVVKNNVQPKIYQNSSTGVGLKNIRGQYLSVFQKDIVIIKS
                     GQTFEVRLPIPD"
     gene            complement(11948..12301)
                     /locus_tag="PLM68_02215"
     CDS             complement(11948..12301)
                     /locus_tag="PLM68_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54219.1"
                     /translation="MSKSSATHLIIHSFALAHALVCYFLHDSSFGDTFLLTCLTIAMV
                     VVLIRLYDGPVEVIVGLLLLASFAGFFLGTKGARLIQTYFPDLKIILSYVVTTTFVTE
                     FLGWSIFFVVRRKKK"
     gene            12478..15000
                     /locus_tag="PLM68_02220"
     CDS             12478..15000
                     /locus_tag="PLM68_02220"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT54220.1"
                     /translation="MNRLQDGWWELRIKPESLPETLFYRYGVSCHDCSVVSEHATHCQ
                     KGLPAGTNSVIIDRWMDPDYSYVFLTHKTTTPKKSDIALITRSVAVPSKCHLAMTGNS
                     EGLGMWNPSRAIPMQYTGSGCWEAAISFSDLGQSAPEYKYIIRRDGQNSLVEWEVGEN
                     RIFPPLPEEDGNNKHLVISDSPYRSVVEGLRYAGTAVPVFSLRSQDSWGIGDFGDLKK
                     MADWASITGQCVIQILPVNDTTMYHNWIDSYPYGGISIMALHPLYANINAMCPKGSSA
                     DLSRFEKKRKALNELPQLDYDKVSALKWEAIRYLFEQTGEKVMKSSSFRTFYKENKKW
                     LQPYALFCVLRDKFGTADFSQWGRYATYDPCFCQEFDNLDVYIFIQYHLDKQLADAHK
                     YLNGKGIILKGDIPIGITPLSVEAWTEPHLFHMDAQAGAPPDEFSVQGQNWGFPTYNW
                     EVMEKDGYAWWKNRFGNMARYFDAYRIDHILGFFRIWTIPKQQTQGLMGYFDPAMPMS
                     AQEVGQWGLIFDQDRMVMPYITDDILFTVFGDKADFVRKKYLDPGKHPGRYHLKEAFS
                     TQRKIALHFSSLKDTEENRILCNSLLDLAANVLFVPQPGKEDAYHPRISAQFNYSYKA
                     LDDNSKFAFNRLYDHFFYKRHNEFWYHQAMKKLPPLITATRMLCCAEDLGMIPDCVPP
                     LMKELAMLSLEVQRMPKDKYRDFGDPASYPYLCVCTTGSHDTSTLRGWWEEDRNITNR
                     FFNTVLGMPGEAPVYCEPWICMQVIRDHLRCRAMLCILPWQDWMSVSGKLRRENPHDE
                     RINVPAIVPHYWRYRMHMTLEELLAEKDFNNDLKNLILESGR"
     gene            complement(15015..15992)
                     /locus_tag="PLM68_02225"
     CDS             complement(15015..15992)
                     /locus_tag="PLM68_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOT54221.1"
                     /translation="MKKIISLLFLLVIALFFVQCKNKENQETWAIVLHGGAGAGKNMR
                     PEVREKYLDLLDQALQTGIQMLEEGASAIDVATRVVVWLEDCPDFNAGKGSVINAEGI
                     HELDAAVMDGKDLKAGAVAGVRDIKNPILAARHVMEDTPHVLLIGEGASAFAASVGLT
                     IVPNSYFSTPERLEQIRRIREKKENPDPKGTVGCVVLDNNGNLAAATSTGGMSGKRWG
                     RVGDVPVIGAGTYANNNTVAVSGTGHGELWIRRCVAFDISALMDYKGYSVEEAAREVI
                     FNKIDKMEGSGGGVICVDKNGRIAMEFNTDIMYRAWATASGQRGTAIED"
     gene            16289..16810
                     /locus_tag="PLM68_02230"
     CDS             16289..16810
                     /locus_tag="PLM68_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54222.1"
                     /translation="MKKTFLLLGAFMLSMIVFSSCDKEQEQVSLLYKEVIYEFTDGVN
                     YFTATLINCNGVRDVNDPSKGTIYATISIKANRSCNVQFISIEGLIDDTYITEYAVFP
                     MVYYTSTPFLQEVTFAKDQVITLNLEFPDVSSRLLKVGRLRVASVINNFKQYAFTVHQ
                     IPYKPASDIIVWN"
     gene            16819..18807
                     /locus_tag="PLM68_02235"
     CDS             16819..18807
                     /locus_tag="PLM68_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOT54223.1"
                     /translation="MDNYRLISQGEIASLKEQRCHSEDWTKVWVAPGFRPERVWDTAF
                     SGDICLGVFETKKELPAGIRIQSGICNAKLHNVSVGDNTCIENIHSYIANYNIGSGCI
                     IENVDKIYTEGMSSFGNGVEVRVLNESGGREVPINNKLTAHFAYILALYRHKTELVKR
                     MQELVSTYSKKVSSDRGTIGDNVTISHSGIIKNVVIGECTNIEGISLLVNGSINGNAH
                     DPVYMGHGVNCEDFIISSGARVESGVNIRKCFIGQGTILGKGYSAHDSLFFSNCFGEN
                     GEASSVFAGPFTVSHHKSTLLIAGMFSFMNAGSGSNQSNHMYKLGPIHQGILERGAKT
                     TSDSYILWPSHIGAFSLVLGRHVNNTDTSSLPFSYLIEQDNTTYLIPGVNLKSVGTIR
                     DVQKWPKRDQRKDPVKLDQINYNLLSPFTIQKMLNGRSILRELMRVSGETTDVFSYKS
                     TKIKNTSLVNGIRYYEIAIKKFLGNSIIKRLEKTHFKSIDEIRERLRPDTTIGLGEWL
                     DISGLIAPKTEIEKLICGIETREIRTIADINRLVEEMHRNYYYYEWTWAYSKIREFYD
                     IDPQKITMDQIKTIVREWEDAVVGLDKLLYEDARHEFSLSSMTSFGADGDLDECRLDF
                     EQVRGIFEKNPFVAAVLKHIEDKTALAKELITRLENNL"
     gene            18831..19019
                     /locus_tag="PLM68_02240"
                     /pseudo
     CDS             18831..19019
                     /locus_tag="PLM68_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4988 a         4432 c         4506 g         5146 t
ORIGIN      
        1 gaacgggtga aagccatatg gcagtggccc caagctggtc tatgtattcg agcttgtctt
       61 tcagtccctg aaaatcgccc cacccgtctc cgtccttatc ggcaaatgaa taaacaagga
      121 gctgataggt gatatcagct ctcttgtttc catcccatgc ccggggagtt tcctttaccc
      181 tggagaatga agctccgtta tccggatcgc ttcccggtgt gggttccttc cgacaagatg
      241 caaagagaag gaaaagggcg caaagtatta ttgcgctcct tttcattgcc ttactgcttt
      301 gtcattgtca gagaagaagt gttggccata ttcagaacga tacggtatgt tccggcttcc
      361 tggaccatta tgttggcacc gttatgggta agattagtta tatcaccacc cagattaagg
      421 tcccagttgt tgttgaagcg gatcttgaat tcattccctg cggccaggtt caaggttgcc
      481 gaataggtca gggtttcctg gtcaaaatcc atggcaacgt ccgttcccca ggcactgcct
      541 tcaaaactgc cgataagacc taccacagtc acaggtgttg ccacggcctt ggtgtcggga
      601 aggtccacac tccagaaata cgttccgttc ggcaacatca tgttgtctcc cgtgtagagc
      661 gtgaattcgt actgggaatc agccacaagc tggcctgcta tccaattccc gctttccacg
      721 atcttgaatc cgtattgcat attgtacatg gttatggcac ctttatacgt tccgtcgccg
      781 gaaccttgca gtttaggatc atcggtcgcg ctccaggaat ggccggaata atcacccgca
      841 actaccactt ctgtgccata ttcagaaacc aggtcccccg tgcgtgtaat ggtcatatgg
      901 tacggtgtgg tggccaggct caggataaca gtatacatgc cggcatctgc tttcaggtta
      961 gctccgtctt gcttcaaatc attcggatcc ccacccaggt taatggccca atcgttgttc
     1021 atgcggaatt tgtattcggt tccggcaggg aatggcagat tggtggctgt ccacaggtca
     1081 gtattcggat cgtaggtcat tggaacatcc tctccccatc cggtgaagct gcctatgaca
     1141 cctaccctgt ctataagggt tgctgtcgca ctcatggtgg taaggtcaac cttcaggtaa
     1201 taatacccgg gagcgggcac ctgaaggtta cctgcagagg gatcggtatc cagcgactcc
     1261 atggaaccgc cgtaattggt gtggttccag tcgggtgcgc tggtgaattt aaagatatcg
     1321 gtaagtgtca cataaccctc gtaatgtcct tcattgtcac cgttaagtac gggtgcggca
     1381 gcaggattcc acccctggtg gtttcctggt acatacattt tttcagggaa actggtgttg
     1441 atgaatttga tggtgtaagg attaaccccc acgttcagca ccaccttgta ttcgcccggt
     1501 gtgcgggtaa agctgatgtt gtctcccttt ccgaagctta cgttatccac tgttcctcct
     1561 gccgaatgtg tccagtcgtg gttcacacgg atcttgaatt ctccttcctg catattggtt
     1621 atggcagaga aagtatgggc tacgggatcg taagtcatat caatgtcatt gacccaccca
     1681 ttgctggcaa cgggtgcaga tcctataagg ctgagtgttt cgacctggat catgtacagg
     1741 gtgttgagct tcttgttcag taggatgtta tacatgcctg agggagcggt tatgtcacca
     1801 ccggttgttc cctttccggt taacgcggta tatccgtttc cgaaagtggt cttctctata
     1861 ttggcgatgg caaagtcatt ttcccatgcg ggtacaggcg agaatttgaa acctacatca
     1921 cctccgcctt ccacactcag gtctatcatt ccttcataca atccctttgt gaccgaggaa
     1981 tgtttgatct ttggagctgt ggcgggattc catccctggt atgctccggg tacgtacatg
     2041 tactcgggat acgttgcaat gtaagtggtg acgttaatgg tgacagtgtt tgaaatgaca
     2101 ccgtccggat aagtggccga gaaagctctg actgtgaaat ccaaatcggc ggcagctgct
     2161 tcaggggccc ctgcggcaac agcggcttcg ttcagtgcat ccactgtgat ggaaaaactt
     2221 gtttccttca gatccgaagc aagaagcaca atcgcagctg tttcaccgta ttgcaaagat
     2281 acactgtagg tgatctcttc attatacccg agccttgccg gttcccaggt aaggagttcc
     2341 agcacgcctt caggatcggt cacatccaga actatcgatt caaccagggc ggacgtttcc
     2401 ggggaaacgg cgtcaccata agcatatatg tcaatggtta ccggatcgga ttcaaatgat
     2461 tcatcctggt ttgtcaccga gacaaagaaa gacatggtaa aggtgtcatt ttccggcagt
     2521 gctggaaaag cactgtacag agctgtcttg aactcggttt ttggtaccct gtagtaaagt
     2581 tcggaggtcg atgccagttt tgttgttgat tctccgaatt tgccgtacag atcataggtg
     2641 agtccttcac ccaggaaacg tgcaggcttc caggaaaaat tcacaaattc atccatcgtg
     2701 ttggaggtca tcagaatatc ggaatgtgca tacatttcag gaggtacggg cgctgtagaa
     2761 aaagcgtctt cactgatcac ctcgcaggag acggctgaca ggctcacgat ggctgcagca
     2821 aataaatatt ttagtatttt cataattgcg tctctttaat catttactgt ttttccataa
     2881 ccagaacgaa aggagtcctg ttgccgtgga gcttcatgat ataggtgccg gtagaaggaa
     2941 cattaatgtc cccgccgcct tttaccagtt tcagtccgcc ttccacacct gtgctggctt
     3001 cgttgttggt tccatattta tcgtcccagc tttcgttcag gcggatcaaa aagttcttat
     3061 caccgctgaa ggtaatggcg ggagataccc acaggttgtt gacttcatcg taaaccatgt
     3121 ccagttcggt accccagccg ctttcgtcaa aggcaccgat gataccgact ttcttaacaa
     3181 gctgtttatc aatggtcatc ttgccgcgtt tgactgtcaa cacgtatatg tttccctcgg
     3241 tcaggtcaac agaaaggttg gagtcggact gttctgggca ataccaggaa ggtgtcagga
     3301 agttgtagcc ccagtttaaa tcggaccagt tgcgcgcaac tgttacctta aagtaggagt
     3361 attgatccga tccgtcttca agatccacca tgatcttgta aacgttggtt ccaccgtctg
     3421 tttcccaaaa ttcaggagcc tgttcaatct tccagccctg gtaattgccg ggcaggtaga
     3481 gtgagcgtaa cgctgcggca aaagtggtaa cattaaatgc aatggagttt gaagtaacgc
     3541 tttccacgtt ggtgccataa actttggcta ccaggtaggc gctgatgctt gccgttgcgt
     3601 tgacacctac tttcaggtcg ttggcaacca ccccattcag gtcagatttt gatacagaca
     3661 gggagttggt aaaagacgag ccgagtaagg cggtctggga gttagccgtt gcgtaaaggg
     3721 aatattccac ctgaacggca gggccaaagg aagcgctgga ccagttgaag gtcacgcttt
     3781 cggttttatt gttatcctcg ttgatgataa ccgagccttg tgaaagcaag accggtgcct
     3841 tgaatccgga aaccggattc agaaatgcct tctcgatctg aatgtcacag gaaacgagaa
     3901 ccagcacaga ggcaaccaac atagtaatat attttgtatg tttcataatc gtaatcattt
     3961 attgaatcct cttatgttag tatcctgtat tctgaaccag attggggtta gcattcagat
     4021 cgctcagtat gatgggatat actttgcggt gttccggtaa ggatactttt ccgttcaaaa
     4081 ccccgccctt gagggtccag ggatattgca tggaagtgaa ataaccgtaa cgtatcaggt
     4141 ctgtcctgcg gtgtccttcc cacatcaact ctcgggcacg ttcgtccaac agccagtcca
     4201 gatccatggt ctcaggtgtc tttgaggcaa ctcctgcccg ttcccgaagc tggttaatgt
     4261 atcccttggc agtggggtcg ttaacgacac cgccgctcat acgggcattg gcttcagcat
     4321 agatcaggta catttcggca agtctgaaga tggggtagtc aatggacgag aatttgtaat
     4381 tcgattcagc atccgatacc gtatgtccgt ctgaatacag accggtgaat ttccagcacc
     4441 tccagccggt atcggtggta gtgttgtcaa aatccttgct tgaaccgatg ttgtagaaga
     4501 aggcacgctt gtcgcttttt tcacggtcat agccaaatcc tgaggttgct ccccattgta
     4561 ctccttcgag ttcaaaacgc tgtacataat catcggctac gtggtaacca ttccaggttt
     4621 cgggattcag caggccactc agacccaggt ttttggcaat ggcattgttg gcatcagaac
     4681 tgagtgttgc agaaaccagg tgggtggtac ctccccagct ttgcgtattg tccttatcgt
     4741 aaacaacagc gtatatgaac tcctgcgttg ctccgtttgt ggtgttgtcc tgcatgaaca
     4801 gctcctggta attggggtgg agcgtgtagc ccattccaat gacagtgccg gcagcatcct
     4861 tggctttctg ccactgtggc gatccggtat atacctcggc attcagatac atgcgggcca
     4921 gtaaagccca tacagatccc ttggtggcgc gaggatacgg gacagcgcct ttggcgggca
     4981 tgtcggtact gttggccagg tcaagcaatt cttcttccaa ccatgtataa agggcttccc
     5041 tgcctatctg cttgggaagt tccccgccaa tgttctcttc aagtgcaaaa ggaggatttc
     5101 cgaacaggtc catgagcagg taataataca aagcacggtt gaagcgggct tcggcacgat
     5161 actggtgtac cgtggcaatt tcggctgagt gtcccctgtc gtttagtttt tcatccgcag
     5221 tctggataag gtactcgttg accagggtaa tgcctttcat gcagcgtgta taaacggcaa
     5281 taatggcttc gttatcggct gatgtccagc gatggtattg aatgggaact atatagttgt
     5341 caccccaaat gattttaaag gagtctgtgg agagttcgtt caaaaccata tacatacggc
     5401 taagctcact ttgtccggca tcggctacgg caatatccgg tgatcccatg tcattctggc
     5461 tcacaagtgc ccagtatcca tatagatagg ccaggccgtt caaataactg gaaggctctg
     5521 cgtatgcggt ctccgaggta gcatctgttt cattcagcgg cagtgtatcc aggtcgttga
     5581 tacacgaggt cagcaggaaa atgaatgcca acgccagcag aaaactgttt ttcaatatct
     5641 ttttcatatc tgccttgtat taaaagttaa tgttaagacg cagggtatag agtctgggac
     5701 gtggtatgat gttgaagtcc acaccgtccg aagacacgat ctcgggatcc agtcccctgt
     5761 atttagtaaa tacatatacg ttctgtaccg atgcagctat acgtatgctt tttgcaaact
     5821 tgaggttgtc aaacgtataa cccaggttaa tatcgtctat gcggaagaag gatgcattct
     5881 cgatgaacat atcagaatat ttctgattgt tttccgatgg ggctgtccat ccctgaacca
     5941 ggtattgttt gctcacgttg ttcagataac ccttgttcca gtcatcgctg taggatgtgg
     6001 aatatcccat ggccaccccg ttgatgatgt agttgccaag ggaaccgtga ccattaagtc
     6061 ccaggtccca attcttgtaa cggaactgtg tgttgatccc aaagaaggca tccggtgtgg
     6121 gactgaaacc ggtaacatag cggtctgcgt ctgtgatctc tccgtcaccg tcacggtcaa
     6181 tcaaaccatt ttgaacggga ttcccttcat tatcgtacaa ttgctggaac atatagaacg
     6241 tgtaaggcgt gaaaccctca cggtgcaggg agctgtaacc gcccgttcct cccatcccgg
     6301 atcctgtggt taccccaagg tagccttcag tcttaagggt ggtcagtttt gtgattttag
     6361 tatcctgaat ggtgatgttg ccgccaatgg accagtgtgt gtcggcagtc tggacaggta
     6421 tgaagttcag ggtgaactcc aatcccttgt ttaccatgtt cccgatattg gatatgatgc
     6481 tgtttccaaa gttggatccc aggggtgtgg atatcacgtt cagcaaatcg taagtattcc
     6541 ggtaataagc ttcggcacta ccggtaatgc gatcgctggc aaaaccgaag tccagaccta
     6601 cgttataggt ttccgtggtt tcccatttca ggttggcgtt gtaagccagg ggtgccagtg
     6661 tggtatagaa accgtcaccc atgaaatacc tcatttctat agcagaagag gaaagatacc
     6721 gggccaggta aggatagtaa tccgaaccga tgtcctgttg tccggtcttg ccccagccca
     6781 gacggagttt cagggctgaa acggctctac ttgatttcag aaaattctcc tggccgatgt
     6841 tccaggcaaa agctgccgag ggaaataagc cccagcggtt ggctttgctg aacctggacg
     6901 aggcatcgtt acgcaaggtt acggtgaaca ggtatttaga ggcgatggaa taattgatcc
     6961 tgccaaagaa agaaagcaaa taatattcct tggcattggt gggtgctgta tagtagtggg
     7021 cacccggacc atccggttcc gtgttcttga attgttcttc atcgtactga acgtagttat
     7081 gctgccagga ataaccggcc atcacatcca ggttgctgtt tctgaaatcg tgattgtaat
     7141 tcaggtatgt ttccagtaat gtattcaggt tgtaattttc ataagtaaca aacaagtcat
     7201 tgctggaacg catggcagag atggagccca gggcgttgtg cctttcaccc catgtgtcgg
     7261 ctatgtcata accagcgttc acatttgccg ttaaatcttc cagtccatgt actttataag
     7321 taagctggac attcccgagt gagcgcaagg tttgattgaa attggtgtag tcgtaaattg
     7381 ttgagagcgg gttggtgctg gccatggtat tggcactgcc atctgcattg aaccagttcc
     7441 agtaaccatt tgcctttgat gtatctatgc tcccgtcggt tttagtaaaa tataccggtt
     7501 tggtcgggtc gaaggccatg gcatttccta tggctcccgt attggcccag ttggtcttct
     7561 ggtaaattcc cttaacgttg acattaacag taaggtgctc tttaaagaac ttgggtgtca
     7621 ggcttatatc cacgttagca cgctggttgt ctccgacctt gagggtggcc tgatccaggt
     7681 tatacccaag gctcagacga tagggcatgt gacgggtcga cccgtagaga ctcacgttct
     7741 ggtccgtgtt aaaggacaat ctgtaaatct ctttttgcca gtctgtattc tccgttccca
     7801 gaagggttgg aatgatctgg ttattgggat acgttgtggc catgaaatca cggtattcat
     7861 cggctgtcat catttggatg gaggaagcgt tctgttttac cgagtaactg gaattgtagc
     7921 tgacgttaaa accctttccc gttcctttct ttgtggttat aatgataaca ccgttggagg
     7981 cccttgaacc atagatagcc gttgcagaag catctttgag gactgtgtag ctttctatgt
     8041 cgttggggtt gacagaagcc agcgggttgc ccattccggc tcctccgtcc ccggtcacgg
     8101 gaactccgtc aatcactatc agaggatcat tggatgcatt caaggatgca gcaccacgaa
     8161 tcctgatggt agcagaagca ccgggctggc ctgttgcagg tgttattcgc agtccggaaa
     8221 cctttccaac cagcatctgg tcaggagaaa ccaccgcacc acgattcact tcttctgccc
     8281 tgatggccac aacagaacct gtcatatcgc tttttttgac agttccatag ccaatgatca
     8341 cgagttcttc cagaagttct gaatcttcgt gcaaaacgat cctggttgcg ttccctgccg
     8401 caacaactac ggtttgatac cccaggaatg atatctccaa cagagtttca ggagatggca
     8461 ctctaagggt aaactgtccg tccagatctg tcgtgacacc gttactggtt ccctgttgta
     8521 gaaccgtagc tgcaatcacc gcttcgccgt taacatcgac gaccgttccc cttacttcat
     8581 actgagcgta aacagaagta acagagaaga gtgtgaccgc aagcaaaaaa gtcatcagtc
     8641 ttttcataaa catttaggtt agttaataat attttagtta gatagtccaa tttttctatt
     8701 ctttctgaaa gcaagctgta ccgggtcatc cctgtcttcc acaaagaata cgcttacagc
     8761 tgcgatcagc agacagagtc cggagaatac catggcaaag atggttttgc ctccgtacag
     8821 gtatttaacc attacccctc ctaccagacc attgattatc tgtggaatgg taatgaaaaa
     8881 attaaaaatg cccatataaa cgcccatctt gtgtgcaggc agcgcacccg ccaggatgga
     8941 ataaggcatg gccaggatcc ctgcccaggc gattcctacc ccgatcatgg ggaaaacaag
     9001 catgcgtggg tctttgatca ggtaaaatga cagaaaaccg atggcaccca tcagcaagca
     9061 caaggcatgg gttcccttac ggctgatttt ttttgatatc attggaagca gaaaagcaaa
     9121 gatggctgcc accccgttgt atactccctg cagtatccct acccagtcac cggcatctgc
     9181 ataggcacgg gaagttgtgt ctgtagttcc gtataaatga tctgcaacag cgggtaccat
     9241 atagacccac atggaaaaca acgcgaacca ggagaaaaat tgaacgaccc ccaattgttt
     9301 catggttttg gggatagata aaaaatcttt tccgatctgc cagaaaggct gtttttcggg
     9361 ccttactcct tcctgaggag gatactccct ggtactaatg actgtccaga gaacgcttcc
     9421 aaataatacg gcagccccta tataaaatgc atatttaacg ttattggcaa caaggtgtcc
     9481 tgcttctaca tccggtgtat tggccatacc aaaccagttg gtcaacaggt aaggaaggat
     9541 ggccccgaga acagccccta ctcctatcag tgatgtctgc acagaaaaac ccattgtccg
     9601 ctgttccgaa ggaagaaggt ccgccaccaa agccctgaaa ggttccatgg caacattgat
     9661 ggaggcatcc atgatcatca ggatcccagc tccaatcagc atgggcgaaa taatgggcgc
     9721 cagcatgggc gcattgggca tcagaacaag tgccagggca gccagaatgg ccccggtcag
     9781 gaaaaaagga cgtctccttc ccaggcgtgt ccacgtttga tcgctgtagt ggccaatgat
     9841 tggctgaaca atcattccgg tcaggggagc agcaatccag aacagggaaa gatggctcac
     9901 atcggccccg aaagactgca aaattcggct gacattggca ttttgcaggg caagcccgaa
     9961 ttgaataccc agaaagccga aagtcatgtt ccagatattc cagaatgtca ttttcttttg
    10021 tgtcatggtc aaggatcaaa taataaattt tatgaattta atctctttac cttaataata
    10081 ttaaagcaaa tccgacgaat tgcataataa tattgaccaa atgcaaaaaa atctatccgg
    10141 attgttagtc tgattccccc tcaatccagc gcatgaaaac agggaccctc tggcggctta
    10201 ctaatatgga ttgtttgtac ggaggaagca gctctatgcg cagccggctg ttaaaaaatt
    10261 tggatataga gttaattgcc gatatactgg caatgcaatt acgggagagc ttgaaaaaat
    10321 ctcttggatt aagctgttct tcaatggtct ccaggctggc atcagagagg taatgctttc
    10381 cctccctggt catgatgtag gtacttttat cctcggaaaa aaagtacgcg atgtcttttg
    10441 tgctaattac aataatctgg tcacctacct taatgacaaa ccgctgtttg aaatcctgat
    10501 attggtttat gagtttgccc agattatgga ggtcctgtgg cctttcaacg gatttgatgc
    10561 atttttcgac tgcttccaca aattcagagt cccccaccgg ctttaaaaga taatccacgg
    10621 cgtttttttt caatgctgcc aaagcataat gttcataagc tgtgactatg atcacaggag
    10681 gcatgggtga aatcctgttg aacaattcaa aacagacacc gtccgaaagt tctacgtcca
    10741 tcaatatcag gtcggcactg tttagtgcca gccacttggc agctttatcc accgagtcca
    10801 ggacagcggc gattatagca tcgggataat aaacactgaa cagccgcttt aactgcatct
    10861 gtgcagggat ctcgtcttcc agtataagta ctttcattgc ctaatccggt attggcaggc
    10921 gaacctcaaa cgtttgcccg cttttaataa ttacaatatc tttttgaaaa acagataaat
    10981 attgtcctcg tatatttttc aatccaactc cggtagaaga gttctgatag atcttgggct
    11041 gtacattgtt tttcactaca aggtggtcat cctctgttgt tatcaaaatt tccaggggac
    11101 tgtctttgct tacaatattg tgtttggtgg cattttccat caacacctgc aggctgcatg
    11161 ggattaccag cctgttcagg tactcttcac ttattttgaa agtaactttc agtgaattgt
    11221 caaaacgctg agacaataaa tctacataaa gctcaacaaa ttccagttcc tccttaagca
    11281 taatggattc ttgttcccct atgttcagaa aatggcggta cagcgaggcc agttttctta
    11341 cgtattcact ggcttttccg gcatcggtgc ggatcaggta gtccaacgaa ttgaggctgt
    11401 taaaaagaaa gtgcggattc agctgatgct taagctgctg atactgatac ctggccttgt
    11461 ttttctggct tatttcaata tccagggctt ttttctgtac ccttccgtag taggcatata
    11521 tgtccattcc ggctatgaaa atcatgttga aaaaaaaagc agcaagggcg gtataccaga
    11581 aattgtaatt gaaaaaagaa ccctcagcag gaccatcccg ggcagacatg attcttacca
    11641 gtatggcaga aaaaagggcg atcacagtaa gtccggatag ttcaagcaat aagcgcaaca
    11701 tgggctttgc tccgtaatct atatgacgta ccagcatcca gaccagcagg aaatcaagga
    11761 ttatcatcag cagggccagc ggatagttac ccaggagccg gctgagtgtt tcgttgaaat
    11821 tcagattgaa attgttttga aaaaaaggaa tggccccttc aaaagccaga cggaatgcca
    11881 acacaaagaa agccagcagc agcacttcta tgatagtggt tcgttttgtt accggtgcca
    11941 ttatccgtta ttttttcttg cgtcgcacca caaagaaaat ggaccagccc aggaattcgg
    12001 tcacaaatgt tgttgtaacc acgtaactca ggatgatttt cagatccgga aaatacgtct
    12061 gaatcaagcg ggccccttta gttcccagga aaaaacctgc aaaagaagca agcagcaaca
    12121 aacccacgat cacctccacc ggtccgtcat ataaccggat tagtaccaca accatggcaa
    12181 ttgtcagaca ggtaagcaga aacgtgtccc caaaagatga gtcatgaaga aagtaacaaa
    12241 ccagagcgtg agccaaggca aaagaatgga taatcagatg tgtagcgctg ctttttgaca
    12301 taatgggtaa agttacaaaa aatactgaac ttttttttag gtatatttgt tacaatcaaa
    12361 taagtcttta tggaaattat ttttcggatt cattacaatg catttcagga aagttacttg
    12421 tttttaagcg gtaacatacc tgaattaggc aatgaaaatt tgaaaaaatc gccacacatg
    12481 aaccggctgc aggatggctg gtgggaactg cggataaaac ctgaatcttt acctgaaaca
    12541 cttttctacc gttatggcgt ttcctgtcat gactgctctg ttgtttccga acatgcaacg
    12601 cactgccaga aaggactccc tgccggaaca aatagtgtaa tcattgaccg gtggatggat
    12661 ccggactatt cctacgtttt tcttacccat aaaactacaa caccgaaaaa atcggacatt
    12721 gcccttatta cacgcagtgt agccgttcct tcaaagtgtc accttgccat gactggcaat
    12781 tctgaaggat tgggaatgtg gaatccgtcc cgtgcaattc ccatgcaata taccgggtca
    12841 ggttgctggg aagctgcaat atccttttca gacctgggac agagtgctcc cgaatataaa
    12901 tatatcatcc gcagggacgg acagaattcc ctggtggaat gggaagtcgg ggaaaatcgc
    12961 atctttccgc ctctgcccga agaagacggg aataataaac acctggtgat cagtgattcg
    13021 ccttaccgta gcgttgtgga aggtttgcgc tacgccggaa ccgcagttcc tgtattttcg
    13081 ttgcgcagcc aagatagctg gggtatcgga gattttggtg accttaaaaa aatggcagac
    13141 tgggcctcca ttaccgggca atgcgttatc cagatattgc cggtgaacga taccacaatg
    13201 taccacaact ggattgactc ctacccctat ggaggcatat ccattatggc cctgcaccct
    13261 ctttacgcaa acatcaatgc catgtgcccg aaaggttcgt cggcagacct ttcccggttt
    13321 gaaaagaaga gaaaggctct gaatgaactt ccacaactgg actatgacaa ggtatcggct
    13381 ttgaaatggg aagccatccg ttaccttttt gaacaaaccg gagagaaagt gatgaaatca
    13441 tcttccttca ggacttttta caaggaaaac aagaaatggc tgcaacccta cgcacttttc
    13501 tgcgttttac gggataaatt cggtacggca gatttttctc aatggggcag atatgccaca
    13561 tacgatccct gtttctgcca ggaatttgat aacctggatg tatacatttt catccagtac
    13621 caccttgaca aacaacttgc cgatgcccat aagtacctga acggcaaagg tattattctg
    13681 aaaggagata ttcccatagg aattactccc ctgagcgttg aagcctggac cgagccccat
    13741 ttattccata tggatgcaca ggctggcgct ccgcccgatg aattttctgt gcagggccag
    13801 aactggggct ttccgacata taactgggaa gtcatggaaa aggacggata tgcctggtgg
    13861 aagaaccggt ttggaaatat ggcacgttac tttgatgcct accgtattga ccatattctt
    13921 ggcttcttca ggatatggac cattccaaaa cagcaaacac agggtctgat gggttatttt
    13981 gatcccgcca tgccaatgtc tgcacaagag gttggtcagt ggggtcttat ctttgaccag
    14041 gacaggatgg taatgcctta tattacagat gatattttgt tcactgtatt cggggataag
    14101 gcagactttg tcagaaaaaa atacctggat ccgggaaaac accccggacg ttaccatctg
    14161 aaggaggctt tttccaccca aaggaagatt gcacttcatt tcagttccct gaaagacacc
    14221 gaagaaaacc gtatcctgtg caacagcctg ctggatcttg cagccaacgt gctctttgtt
    14281 cctcagccgg ggaaagaaga tgcctaccat ccaaggattt cggcccagtt caactattcg
    14341 tacaaggccc tggacgataa ttccaagttt gcgttcaacc gcctctacga ccacttcttt
    14401 tacaaaaggc acaatgagtt ctggtaccac caggccatga aaaaactgcc tccccttatc
    14461 acggcaacaa gaatgctatg ctgtgccgaa gacctgggca tgatacccga ttgtgtgccg
    14521 ccactgatga aagaactggc catgctcagc ctggaggttc agcggatgcc taaggacaag
    14581 taccgggact ttggcgatcc tgcctcttac ccatatttat gtgtttgtac tactggctcg
    14641 cacgacacca gcaccctgag gggttggtgg gaagaagacc gcaatatcac caaccggttt
    14701 ttcaatactg ttcttggaat gcccggagag gctccggttt attgtgaacc ctggatttgc
    14761 atgcaggtta tcagggacca ccttaggtgt cgtgctatgc tgtgcatact gccgtggcag
    14821 gactggatgt cggtttcggg aaagttgcgc cgggaaaatc cccacgatga gcgcatcaat
    14881 gtgccggcca ttgtacccca ttactggcgc tacaggatgc acatgacact ggaagagctc
    14941 cttgcggaga aagatttcaa caacgatctg aaaaacctga tcctggaaag cggccgctga
    15001 aagtcacagg tggcttaatc ctcaatggcc gtcccgcgct gcccgcttgc cgtggcccag
    15061 gcccggtaca tgatatctgt attgaattcc atggcaattc tgccattttt gtcaacacag
    15121 atcacgcctc cgccggagcc ttccatctta tctatcttat tgaatatgac ttcacgggca
    15181 gcttcttcca cagaatagcc tttatagtcc atcagggcgc tgatatcgaa ggccacacac
    15241 cttctgatcc ataattcccc gtgtccggtt cctgaaactg caactgtatt gttgttggca
    15301 tatgtgcctg cgccgatgac aggcacatct cccacccttc cccagcgttt accactcata
    15361 cctccggtag aggtagctgc cgccaggttg ccgttattat ccaggacaac acaccccacc
    15421 gttcctttgg gatctgggtt ttccttcttt tcccggatcc tgcggatctg ctccagcctt
    15481 tcgggggttg aaaagtaaga attgggcact atggtgagcc ccacggaggc agcaaaagcc
    15541 gaggctcctt cccctatgag caacacatga ggagtgtctt ccataacatg cctggcagcc
    15601 aggatgggat tctttatatc acgcacgcct gcaactgctc cagccttcag atctttccca
    15661 tccatcaccg ccgcatccag ttcatggatt ccttcagcgt tgatcacaga tcccttcccc
    15721 gcgttgaaat cagggcagtc ttccagccag acgactaccc gtgtggccac gtctatggca
    15781 gatgcaccct cttcaagcat ttggattcct gtttgcaggg cctggtccag caggtccagg
    15841 tatttttccc tgacttcggg cctcatgttt tttccggccc ctgctccacc gtgaagcaca
    15901 atggcccatg tttcttgatt ttctttgttt ttgcattgca cgaaaaataa tgcaatgacg
    15961 agcaaaaaca gcaaggaaat gatttttttc atgacaaggt tcttagttat tttctgttta
    16021 caaggtaaag attttttgat atttcagaaa aaagttttac attagtcgta ccatttatat
    16081 gtgaccttgg atacggattt attaatgata cgataggtgt ttcttatcgt ttttagttca
    16141 aggaaccgat ttcgccgaaa gccgtctaaa cagggcggct ttcttttttt aagagatgca
    16201 gcaaagcaaa attatcttgc gtctgatatg catatccgtt atttatgtta tttttgacct
    16261 tgataataat ccttttcaaa ataatcttat gaaaaaaact ttccttttac ttggtgcatt
    16321 catgctttct atgatcgtgt tctcttcctg cgacaaagag caggagcagg tatcactttt
    16381 gtacaaagag gttatttatg agtttaccga tggtgtgaac tattttacag caacattaat
    16441 caactgcaat ggagtgaggg acgtcaacga tccctccaaa ggaacaatat atgctacaat
    16501 aagcattaaa gctaaccgtt catgtaatgt acaattcata agtattgaag gactgattga
    16561 tgatacgtat attactgaat atgctgtttt tccaatggta tattacactt caaccccctt
    16621 tttacaggaa gtaacatttg ccaaagacca ggttattacg cttaacctgg aatttccgga
    16681 cgtttcctca aggttgctga aagtaggcag attgagggtt gcatcggtaa tcaataattt
    16741 caagcaatat gcttttacag ttcaccagat accctataag cctgcaagcg atataattgt
    16801 ttggaattaa gctgatttat ggataattac cgtcttatca gccaggggga gattgccagt
    16861 cttaaggagc agcgttgtca ttccgaagac tggaccaaag tctgggtagc gcctggtttt
    16921 aggccggaaa gggtctggga tactgctttc tcaggggata tctgtctggg tgtttttgaa
    16981 acaaaaaaag agctccctgc cggaatacgg atccagtctg gtatctgtaa tgccaaattg
    17041 cacaacgtat ctgtaggtga caatacttgc attgaaaaca tccacagtta tattgcaaat
    17101 tataacattg ggtccggttg tatcatagaa aatgttgaca agatctatac ggaagggatg
    17161 tcctcattcg gtaacggtgt ggaagtacgt gtccttaacg aatcaggcgg ccgggaagtt
    17221 cctataaaca acaagcttac tgctcatttt gcttacatcc ttgcattata ccggcataaa
    17281 acagagctgg ttaaaagaat gcaggaactt gtaagcacat attccaaaaa agtatcatct
    17341 gacagaggaa ccatagggga taatgtcacg atcagccatt cgggaataat aaaaaatgtt
    17401 gtaataggag aatgtacgaa catagaagga atctccttgc tggttaacgg aagcatcaat
    17461 ggaaatgcac atgaccccgt ttacatgggc cacggagtga actgtgaaga ttttatcata
    17521 tcctccgggg ccagggtgga aagcggggtt aacatcagaa aatgcttcat aggccagggg
    17581 actatactag gaaaaggcta ttctgcgcat gattcgctgt ttttcagtaa ttgtttcgga
    17641 gaaaacgggg aagcctcttc ggtatttgca ggacctttta ccgtcagtca tcataaatcc
    17701 accctgctca ttgcaggcat gttttctttc atgaatgcgg gttcgggttc taaccagtcc
    17761 aaccatatgt acaaattggg gcccatacac cagggcatcc tggaaagagg cgctaaaacc
    17821 acttccgatt cttacatcct atggccatcg catatcgggg ctttttctct tgtcttggga
    17881 aggcatgtga acaatacaga caccagttct cttccttttt catacctgat agagcaggac
    17941 aacacgactt acctgattcc cggagtcaac ttaaaaagtg tggggaccat acgggatgtc
    18001 cagaaatggc caaaacggga ccagagaaaa gatcctgtca aactggacca gatcaattac
    18061 aacctgctga gtcctttcac gatccagaaa atgctaaacg gcaggtccat cctcagggag
    18121 ttgatgaggg tctcgggaga gacgacagat gttttctcat acaaaagcac caagatcaaa
    18181 aacacctcac tggtaaacgg tatcaggtac tacgaaattg cgatcaaaaa gttcctggga
    18241 aactccataa tcaaacgtct ggaaaaaaca catttcaagt caatagatga gatcagggaa
    18301 cgtttgcgtc cggatacgac aatcggtctg ggtgaatggc tggacatatc gggccttata
    18361 gccccaaaaa cagaaattga aaaattgatc tgcgggatag aaacccgtga aatccgcacc
    18421 atagcggata tcaaccgcct tgttgaggaa atgcacagga actactacta ttacgaatgg
    18481 acttgggcgt attccaagat cagggaattc tatgatatag atccacaaaa gatcaccatg
    18541 gatcaaatta aaacgattgt cagggaatgg gaagacgcag tagtgggatt ggataagttg
    18601 ctctatgaag atgcccggca cgaattttca ttaagttcca tgacaagttt cggagccgac
    18661 ggagatctgg atgaatgccg ccttgatttt gaacaggtaa ggggtatttt cgagaagaat
    18721 ccatttgtgg ccgcagtgtt aaaacatata gaagacaaga cagccttggc gaaggaactg
    18781 ataaccagat tggaaaataa tttatgaact ctgttgacga aaagctattc ttagcacgtc
    18841 agaccataat aaaagacact ttcttttcca ttatcaatgc tcaaaaaaac gatctgacct
    18901 gtgagtgtgc caacctgaat gccgatacac ctgcgggaat gatgatgaaa ttttccagtg
    18961 agacaagcag gtattttgta tcggattacc ttttgttcga ggaagtgatc aatgctgtcc
    19021 gccggggctt gccccggcta agccctccct acggctaggg agggtttggg tg
//
LOCUS       DAOSOF010000021        18820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2946_length_18820_cov_4.738449, whole genome shotgun sequence.
ACCESSION   DAOSOF010000021 DAOSOF010000000
VERSION     DAOSOF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18820
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2946_length_18820_cov_4.738449"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..855
                     /locus_tag="PLM68_02245"
     CDS             202..855
                     /locus_tag="PLM68_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOT54224.1"
                     /translation="MNDFPLNLLLGMIAGFSVSMPLGPAGLFCIQRTLSKGQRSGLVA
                     GLGAATSDIIYALLAILSLSFINNFIENNKNLLFFISGLGLMIIGIAIYSTNPVKQLR
                     QNRESKKFVEDYFTGFLMSITNPGCLFLIIGVFAFLGTTVDRSSGSFIISAILLGVFL
                     GGSLWWFFLSTIISLFRKKIRLRQLWNINRFAGIALMVLGMVAGTKGLSGLLRTLIH"
     gene            884..1285
                     /gene="ssrA"
                     /locus_tag="PLM68_02250"
     tmRNA           884..1285
                     /gene="ssrA"
                     /locus_tag="PLM68_02250"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1315..2250
                     /locus_tag="PLM68_02255"
     CDS             1315..2250
                     /locus_tag="PLM68_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3836 domain-containing protein"
                     /protein_id="HOT54225.1"
                     /translation="MRKVHLILLAFILTGCMNNSWWEPRTLITNDKNGQPLGRYQFTY
                     DERGNKFTETGDIWDQTTATWIRDYRISYSYDIYGVRTGALKEKRDSLSAKWLPVSRV
                     ICNYSQETYRPETETHQVFLADKQLWEDSFRDHYIHDANGRLLYIVREKWNNGEFDRD
                     IALRGKFLIKAHVITPVTGWENSIRDSYFYDDEGVMTGDQVDKWDRVNNTWVPAFRYT
                     QTRDRKGNIVVEDEEKWDEDNGSWSLMGQYTYLCDNHGNTVETRYIPVTEDPEASYYL
                     VFYYNNRQSLIGYYIGDTGTFPGHSGTAEYFRLKR"
     gene            2247..5003
                     /locus_tag="PLM68_02260"
     CDS             2247..5003
                     /locus_tag="PLM68_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT54226.1"
                     /translation="MSPVSFLSSVAGKLYKTYGDRISDCCLVFPGRRAGLFFQKELSA
                     CLERDIWMPSLLGIKQLTESITGKKTTENLFLLTELFRLYRELTGKDESLQGFYSLGN
                     IILHDFDQTDKYLLKAGDLFHNVRDLKRISQDFSFLSHEQQQLLKSFWSAFQVKPDHL
                     LNQHFISVWEQLPLLYELFNKELRKKDFCYEGMIYRQMAEDLEGEKALDFFHKYYRFV
                     FIGFNALNTCEKKLFRYLRDNKKADFFWDYDPYYIEDPLQEAGLFLRENLKEFPPPGG
                     YDKECSPLSGHRNITVVPVTSYVMQAKIVPQLIKDNKLPADTRTAVVLGDESLLLPLL
                     YGLGQQEDTSGEINVTMGFPLRQTALFGLFDSLLRFYDLHGTEKDFRPVSSHPYVETL
                     GISTFLYKVPEDPAELYSIFLEILDKIDGSDTLPANDVLLLPVVREARKLLNKLHLAI
                     SRTSTEISTPVFAKLIRQHLSLATIPFSGEPLTGLQVMGFLETRCLDFENVVIVSAQE
                     NVLPKMFKDNSLIPRNIAKAFGLPSAEQHIAMQGYYFYRLLQRAKNVFLIWNNNPEGP
                     ARGEISRFVRQISCELPDSPVKTTPLVYDHTLPETKPVTIAKTGEVKEQVEEYLYPEG
                     KKALSPTALQTYIRCPLKFYFKHVLSLKESHEIAEEADSLHIGNIVHTVLFDLYKPYI
                     GKMLQKEELLRMIRPVANLRRRVAVVAEEYMNKKSKEGLHIDPGRQLLFSEVATLYTE
                     RFISHDAGQSPVHVFDVEKKFGCRYGKAFIAGVIDRIDTRTNHLTLIDYKTGSEKNTF
                     SRVEDLFDSRLSGKNGDAFQILCYVFLYSRTVNTEVLPLPLLFYLNKPFSHKTNGYLR
                     YQRTGLNDQQVLAELQKGFLAQLNLLLEELFNFNVPFRQTDFIDNCKNCTFISICQRE
                     KYND"
     gene            4996..7029
                     /locus_tag="PLM68_02265"
     CDS             4996..7029
                     /locus_tag="PLM68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT54227.1"
                     /translation="MTKKEKEKILLPVLFILAAFLMVISLPREGQFKYQYQKGSPWMY
                     ENLDATFDFPILKTEEELRLEREQISRNFTPYFSLKPDIEAAQLRTFTIDAVQAGLSD
                     ALQGKMLTQYKTIYGRGVMDDVRYDALNPETGIAVIQGKMAEKKAATEVFSLSSAKDY
                     FLNDMKIFTEQERKEMRSTGIDFSVFLVPNLIYDESTTIAVQASSLQDISLTKGIVHT
                     GQRIVSKGEVITGTTEQILNSLRAEFNSKIGFSGNIFLLILGQSMIIILSLFILFILI
                     QATRPRVLQNLPELVFILFLFVIVVAGSSFIYKLEPSALYLLPFPVIVLFMDSFFPTR
                     FSLPLYILFLIPLAMITESYHLPLINIVAGSVAIYIFRIWGRGWQQFLSALMVFIAYL
                     FIDLAIHLISEGTFERLTWGVFRNYGVASILMIASYPLVFLFEKIFGFVSVSRLRDLA
                     DTGNKALRELSEKAPGTMQHSLQVANLAETAARTIGADALLCRVGSLYHDIGKINNPQ
                     YFAENQTASFNPHSLLNPRESARIVIQHVEDGKNLAKKFGLPALVSDFIDTHHGKTRT
                     ESFYTAWCNEGGDPANTEDFTYEGQLPTTPEHVVVMLADAVEAACRSLKDYTPESISA
                     MVNRMVRLRVSEDQLQNADITYRNLRRIQENLKVQIQEMYHSRITYPKRKRLK"
     gene            complement(7021..7911)
                     /gene="menA"
                     /locus_tag="PLM68_02270"
     CDS             complement(7021..7911)
                     /gene="menA"
                     /locus_tag="PLM68_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOT54228.1"
                     /translation="MLPVLQSIRPRTLLLSVASILAGTFLAKSQGHFSWPLFLMALLT
                     ACLLQSLSNLVNELGDWKKGTDRDQAGRKSLSLQSGRLTERQLLIIIIIVAVLSLLSG
                     LGLIRMAFGTLREMSSLLFILLGASALAAAVFYSAGKKPYGYYGAGDIAVFLFFGLAG
                     VAGSYYLFTGSLDPAVLYIASAIGMLITAVLNINNIRDFENDKRFGKNTFAVFLSRFS
                     KNGSTFPAKIYQVVLISLAMFLSVLYACKPGTGTFLFLICLPLLGIHILWVIRKEGPA
                     LDNALKILIVAVLVYALSLF"
     gene            complement(7918..8700)
                     /locus_tag="PLM68_02275"
     CDS             complement(7918..8700)
                     /locus_tag="PLM68_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT54229.1"
                     /translation="MNIKCLLIDDEPLALQQLVSYVGKIPYLELVKACSGPTEAMEVM
                     ANEKVDVIFTDIEMPDINGLEYIRSLKDKPIIVFVTAYANFAIEGFQVDATDYLLKPF
                     SLTDFMRCAEKVKQQHDLRMAATSGKEKPAGADKKETSESDEKKATQGWENEYVFVRA
                     DSRTVRLKISDIQYIESRKEYVVIFSQNSLPVMTLFSLKSIEEKLPKDVFMRVHRSFI
                     VNLTKITEVVQNRIVFGKDASVPLGDQYKKDFQNFLNQNFLK"
     gene            complement(8710..9744)
                     /locus_tag="PLM68_02280"
     CDS             complement(8710..9744)
                     /locus_tag="PLM68_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT54230.1"
                     /translation="MTKNREYDISRELKKLRGLENLTLWGIIGGAFLSPLFIQQQIQP
                     LLYILLPFLIGLTIHHYVYVPLFLLKGHYFTYFFLLFFLLLGIVFTCEAVIDILDEKF
                     DITWTNLLPYESTLIGNFVIATLLMGMNVAIRLIFDNIKNKMLAHEADNKLMNYKMEF
                     LQFQISPHFLMNTLNNIHVLVDTDEVKAKEAIVSLSRLLRHMLYESTPDSKISFEKQL
                     TVLKAYCDLNLLRYGENVKLNITVPRYIPNIQLPPLIMIVFIENAFKHGVVYGEETHI
                     NMNFWEEPGFFHFSISNTIFPDKPKNTNPGGLGIKNVKERLNLLYGDSYKLTMEQTEK
                     DYIIHLQLPI"
     gene            complement(9998..12925)
                     /locus_tag="PLM68_02285"
     CDS             complement(9998..12925)
                     /locus_tag="PLM68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT54231.1"
                     /translation="MSRLHKFTTAVLTLIAFGVILSCGPRYSYQAVEGDPLNARIYTL
                     DNGLKVYMTVNKEIPKIQTYIAVRVGSKNDPSETTGLAHYFEHLMFKGTSKFGTQNYE
                     LEKPLLDQVELLFEEYRTTADPEQRKAIYKRIDSISLEASRYSIPNEYDKLMSAIGST
                     GTNAGTSYDQTVYIEDIPSNQIKNWAKIQAERFSDNVIRGFHTELETVYEEMNMYSTM
                     DSEKVIDKLFASLFPSHPYGTQTVIGTQEHLKNPSITNIKNYYTTYYVPNNIAICLSG
                     DFDPDEMIAVIDEYFGSWEPNPNLPEHVLTQEEPVNGPLTVEVWGLESEYVTLGWQGP
                     HAASMEDALFTVLGEVLNNGRAGIMDLDLNQQQKVLQSGAFAYMLSDRGTLVLQGNPK
                     QGQSLDQVKELMLGCLDKVRRGDFEESMLKAIVNNYKFNMMRRLESNRGRANMFVSAF
                     INGIPWEQNVRFIDRMEAVTKEDLVAFTNQYFKDDNYAVIYKRQGPDTSYKKIEKPKI
                     TPIFTNRDTSSAYLKEIVSSVVEPIEPVFVDFEKDYMKDTLREGINFIYVPNTLNDLF
                     TLTFVYEIGSINDRALPVAFDYLNYLGTADKTADQISTAFYELACTFSTYVSDNTCTI
                     TLNGLSENMQPALELLIELITGAQADPDVYKTLTANILKSRSDAKGDQRTNFQRLQNY
                     VQYGPINPSTNIVPDNELKRTDPTLLIQRIQDLLKYEADVYYFGPATMDQAGAVITTS
                     FPMEEALVPAPEKKYFQRQITKENTVYLAQYDANQMYYATYSAREGETYANVPYSVAR
                     MYNEYFGSSMNAIVFQEMREARGLAYSANARFVEPARNEEGFPYYFTSFIATQNDKMA
                     EAMKAFEEIINNMPESEKSFSLAKEGLMSTLRTERITGRNLLNYYRQAKEKNLWTDMR
                     KVVFEEAASLTLQDVVAFQREWISNRPVNYCILSDIKALDMKQLANYGKIVRLSTKEI
                     FGY"
     gene            13008..13703
                     /locus_tag="PLM68_02290"
     CDS             13008..13703
                     /locus_tag="PLM68_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT54232.1"
                     /translation="MQATSPLSMYWIILIGTMLLSWLVQAILNSKFTKYSKIAISSGI
                     TGAEVARKMLQENGITNVLVTSTRGRLTDHYNPVTRTVNLSEGVYNSKSIMAAAVAAH
                     ESGHALQHAQGYAPLKMRSALVPIVNFASQWVMWVIVAGMLVINTFPQLMLAGIILFA
                     LTTFFSLITLPVEVNASQRALAWLNNTRITNYETYPMAKDALKSAAYTYFIAAISSLA
                     TLFYYITLFSRRR"
     gene            13755..14657
                     /locus_tag="PLM68_02295"
                     /pseudo
     CDS             13755..14657
                     /locus_tag="PLM68_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            14669..15345
                     /locus_tag="PLM68_02300"
                     /pseudo
     CDS             14669..15345
                     /locus_tag="PLM68_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
     gene            complement(15311..16721)
                     /locus_tag="PLM68_02305"
                     /pseudo
     CDS             complement(15311..16721)
                     /locus_tag="PLM68_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155722.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16781..17758)
                     /gene="uxaC"
                     /locus_tag="PLM68_02310"
                     /pseudo
     CDS             complement(16781..17758)
                     /gene="uxaC"
                     /locus_tag="PLM68_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            complement(17774..18214)
                     /locus_tag="PLM68_02315"
     CDS             complement(17774..18214)
                     /locus_tag="PLM68_02315"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOT54233.1"
                     /translation="MRSRRKILAITALLLFLLMPLVILLFDPGAANLQARQSFCPFKM
                     LTGLPCPGCGMVKSMVSLYRGEWIESLEFHLFGPVLVLFFACALVVMTAGLIRGKDYS
                     FRWLYNPRVTWSVAIILGVWHLTRLVSFILTHDLDQILRESIWA"
     gene            complement(18222..18578)
                     /locus_tag="PLM68_02320"
     CDS             complement(18222..18578)
                     /locus_tag="PLM68_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOT54234.1"
                     /translation="MQIIDNIPEFKREPLVVLLLGFVTCGLYLIYWNLQAAKVFNALS
                     GREILSPVVAVFAGCIWPVNMYYFFIVGRDGMPALDQRLGIPERDQSILLMLLGFFFP
                     MIAAMIVQSEINKLYR"
     gene            complement(18590..>18820)
                     /locus_tag="PLM68_02325"
     CDS             complement(18590..>18820)
                     /locus_tag="PLM68_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HOT54235.1"
                     /translation="RGFDQRLRYGGEDREFGERLERLGIRGRQIRYSAVTLHLDHPRD
                     YIHHSSWEFNRKVRRENRRNKIIETEYGLIID"
BASE COUNT         5133 a         4337 c         4229 g         5121 t
ORIGIN      
        1 ccggatatag tgaaagcatg ttccatccat aaaagaggta caaaggcgaa taacatgatc
       61 agtcctgtgt gcggaaccag aaatgggata cttagaagaa tgcccgataa tccggataat
      121 gtaaggttct tacgtataga attcattaga caaatctact aattataatt aatttttata
      181 tttttgataa ctttgcccgt catgaatgat tttcccctga atctcctgtt aggaatgata
      241 gccggttttt ctgtatcaat gccactggga ccggcaggtt tattctgcat tcaaaggaca
      301 ctcagcaaag ggcaacgttc aggattggtg gcaggccttg gtgctgccac ttcggatatt
      361 atatatgcat tactggccat tctgagtctt tcctttatta ataacttcat tgaaaacaat
      421 aagaatcttc tttttttcat aagtgggttg gggctgatga taataggaat tgcaatttat
      481 tcaactaacc ctgtcaagca attgaggcaa aaccgcgaat ctaaaaaatt tgtggaagat
      541 tattttaccg gctttctcat gtcaattacc aatccaggat gtttgttcct tatcataggg
      601 gtgtttgctt ttcttggcac caccgtggac cgcagctcgg gctcttttat catatcggcc
      661 atcttgctgg gggtgtttct gggtggttcc ttatggtggt tctttctgag caccatcatc
      721 agcctgttca gaaaaaaaat aaggctcagg caattatgga atatcaaccg ttttgccggg
      781 attgccctca tggttcttgg aatggtggca ggaacaaaag gcctctcggg cctgctccgt
      841 accctcattc attgatattt ttacctatct ttgcattcac aatggggatg ttctggcttt
      901 gacagcagac gacattggaa agcaagcacg tggagcttcg tgtcccggct ccgtaatatc
      961 aggactcagg ccataactgg caacaacaat tatgcgctcg ctgcctgatc gtccaaagga
     1021 ctgatcgctt aatctctccg ggccaggccc ggggcgacga gtatcccgca gaaatttccg
     1081 ccctccgggt ttttgcatat ggggtatcat ctacgagggg ctggttgtaa agattctccg
     1141 gctttgcaat aagatttgag gagatacggg gaatatggcc cgcctgcagg cagtatccct
     1201 cccgacaaaa aaatgtgggc taaacgtgta gaaaactttt ggatgcgctg tttggacggc
     1261 ggttcgaatc cgcccatctc cactacaaaa ggaatggatt taaaacagac aatcatgaga
     1321 aaggttcact taatcttact ggcatttatc cttaccggct gtatgaataa ttcatggtgg
     1381 gaaccccgta ctctcatcac caacgataag aacggccagc ctttgggcag gtatcagttc
     1441 acatacgatg aacggggaaa caaattcaca gagaccggtg acatttggga ccaaaccact
     1501 gcaacatgga ttagggacta tcgtatatcc tattcatacg atatctatgg agtccgcacg
     1561 ggagccctga aagaaaaaag agactcatta tccgcaaagt ggcttcccgt atcaagagtc
     1621 atctgcaact attcccagga gacttatcgt cccgaaactg aaacccacca ggttttcctc
     1681 gcagacaagc aactttggga agattcattc agagaccact atatccatga tgccaatggt
     1741 cgtctgcttt atattgtccg tgaaaaatgg aataacgggg aatttgacag ggatatagcc
     1801 ttacgcggta agttcttaat caaagcgcat gtgattaccc ctgttaccgg ttgggaaaac
     1861 tcaatcaggg attcatactt ttatgatgac gagggtgtca tgacaggaga ccaggttgac
     1921 aagtgggatc gtgtgaacaa tacatgggta cctgctttcc gctatacgca gactcgcgac
     1981 aggaaaggaa acatagtggt ggaagatgaa gaaaagtggg atgaggataa cggttcctgg
     2041 tcactgatgg gacaatacac ttatctgtgt gataatcacg gaaatacagt ggaaacccgt
     2101 tacatacctg tgacagagga tcccgaggca tcctattatc ttgtattcta ctataacaac
     2161 aggcagtctc tgattgggta ctatataggc gatacaggga cattcccagg gcatagcggc
     2221 acagccgagt atttcaggct gaaaagatga gccccgtttc tttcctgagt tctgttgccg
     2281 ggaagctcta taaaacatac ggagacagaa tatctgactg ttgcctggtt tttccaggac
     2341 gcagagccgg tctttttttc caaaaggaac tatccgcctg tcttgaacgg gatatctgga
     2401 tgccttccct tcttggcata aaacaactta cagaaagcat cactggtaaa aaaacgactg
     2461 aaaacctgtt cctgctcaca gagttgttcc ggctatacag ggagctgacg ggcaaggacg
     2521 aaagcttgca ggggttttat tcgctgggca atatcattct tcatgatttt gatcaaaccg
     2581 ataaatacct gctcaaggca ggcgatttgt tccataatgt aagggacctg aaaaggatca
     2641 gccaggattt cagtttcctt tcacatgaac agcaacaact ccttaagtct ttctggtccg
     2701 cattccaggt caagccggat cacttgctga accagcattt catatcggta tgggagcaat
     2761 taccgctatt atacgagttg ttcaacaaag aactcaggaa aaaggacttc tgttacgaag
     2821 gaatgatata caggcaaatg gcagaagacc tggagggaga aaaagccctt gatttcttcc
     2881 ataagtatta caggtttgtg tttatcggct tcaacgccct caatacctgc gaaaaaaaat
     2941 tgttccggta cctgagagac aacaaaaagg ctgacttttt ctgggattac gatccctatt
     3001 atattgaaga tcccttgcag gaagccggtt tgtttctcag ggaaaatctc aaggaattcc
     3061 ctcctcccgg gggctatgac aaggaatgct ccccgctgtc aggacacagg aacataacag
     3121 tagtgcctgt tacttcctat gtcatgcagg ctaaaatcgt tcctcagctg attaaggata
     3181 ataagctccc tgcagacaca agaactgccg tcgtactggg ggacgaaagc cttcttttgc
     3241 ctctgctgta cgggctggga cagcaggagg atacatcagg ggagatcaat gtgaccatgg
     3301 gttttccgtt gcgtcagaca gctctcttcg gcctgtttga ctcactgttg cgtttttatg
     3361 acctgcatgg aactgaaaag gacttcaggc cggtaagttc ccatccttac gtagaaacac
     3421 ttggtatttc tactttttta tataaagtgc ctgaagatcc ggcagaattg tactccatat
     3481 ttcttgaaat cctggacaag attgacggat cggacacgtt gcctgcgaac gatgtgttac
     3541 tgttgcctgt tgtccgggaa gcaagaaagc ttctgaacaa gctgcacctg gcaatatctc
     3601 gcacctctac tgagataagt acgcctgtct ttgccaagct gatcagacaa cacctttccc
     3661 tggccaccat tcctttcagt ggcgaaccac ttacaggact acaggtaatg ggattcctgg
     3721 aaacacgttg cctggacttt gagaacgtgg tcattgtttc tgcccaggag aatgtgctgc
     3781 caaaaatgtt caaagacaac tccctgattc cgcgtaacat tgccaaagca tttggccttc
     3841 cttcggctga acagcatatt gccatgcagg gttattattt ctacaggtta ctgcagcggg
     3901 caaaaaacgt attcctgatc tggaacaaca atcccgaggg ccctgctcgg ggtgagatca
     3961 gtcgttttgt gcgtcagata tcctgtgagc ttcccgattc ccccgtgaaa acaacgcccc
     4021 ttgtctatga ccataccctg ccggaaacca agcctgttac catcgcaaaa acaggtgagg
     4081 tgaaagaaca ggtagaggaa tacctgtatc ctgaggggaa aaaggcgcta agccctaccg
     4141 ctttgcaaac gtatatcagg tgccctctga agttttattt caaacatgtg ttgtccctca
     4201 aagaatccca tgaaattgca gaagaagctg attccctgca tataggaaat attgtccata
     4261 cagtgctgtt tgatctctac aaaccgtata tcgggaaaat gctgcaaaaa gaagagcttc
     4321 tccggatgat ccggcccgta gcaaatctac gccggcgtgt agccgtggtg gcggaagaat
     4381 acatgaataa gaaaagcaaa gaagggttgc atatagatcc gggcagacag ttgctgtttt
     4441 ccgaagtggc caccctctat accgaacgtt ttatcagtca cgatgcaggg caatcaccgg
     4501 tacatgtatt tgatgttgaa aaaaagttcg gctgccgcta cggaaaggct ttcattgcgg
     4561 gtgttattga caggattgat acccgtacga atcatctgac cctgatagat tataaaacag
     4621 gttctgaaaa aaacaccttt tcccgggttg aagatctgtt tgactccaga ttatcaggaa
     4681 aaaacggaga cgcattccag attctctgtt atgtattcct gtacagcagg actgttaaca
     4741 cagaggtact tcccctgcct cttttatttt acttgaacaa acctttcagc cataagacaa
     4801 acgggtacct tcgttatcag agaaccggcc tgaacgacca gcaggtcctt gctgaacttc
     4861 agaaagggtt tcttgctcag ttgaacttgc tattggaaga attgtttaat tttaacgtgc
     4921 cgttcagaca gacggatttt attgacaatt gcaaaaactg caccttcata tccatatgcc
     4981 aaagagaaaa atacaatgac taaaaaagaa aaagagaaaa tcctcttacc ggtacttttt
     5041 attctggctg ctttcctgat ggtcatctcg ctgccccggg aaggccagtt caaataccaa
     5101 tatcaaaaag gcagtccctg gatgtatgaa aacctggatg caacttttga ttttcctatt
     5161 ttgaaaacag aagaggaatt gcgtctggaa cgtgagcaaa tctcaaggaa cttcactccg
     5221 tatttttcat tgaaacccga tattgaagcc gctcagctga gaactttcac aatagatgca
     5281 gtacaagccg gactctcaga cgcgttgcag ggaaaaatgc tgacccagta taaaacaata
     5341 tacggcaggg gtgtcatgga tgatgtccgt tacgatgcac tgaatccgga aacagggatt
     5401 gccgtcatac aagggaaaat ggcagaaaaa aaagctgcca cggaagtttt ctccctgtct
     5461 tctgccaaag attattttct caatgatatg aaaattttca cagagcagga acgcaaagaa
     5521 atgagatcta caggcatcga cttttctgtt ttcctggtac ccaacctgat atatgacgaa
     5581 agcaccacca ttgcggtaca ggccagcagc ctgcaggata tttccctgac caaaggaatc
     5641 gtccacacag gccagcgtat tgtgagcaaa ggagaagtaa taacgggtac tactgaacag
     5701 atactgaact cgctccgtgc agaattcaac tcaaaaatcg gtttttccgg gaatatcttc
     5761 ttgctgatat tggggcaaag catgataatc atactttcgc tttttattct gtttatcctt
     5821 atccaggcta ccaggcccag ggtgcttcag aaccttccgg agctggtttt catattattc
     5881 ctgtttgtta tagtggtggc cggttcgtca tttatctaca aattggaacc ttctgcctta
     5941 tacctgttgc cattcccagt catagtactt tttatggatt catttttccc cacgcgtttc
     6001 tcccttcctt tgtacatatt attcctgata cccctggcga tgatcacaga gagttatcat
     6061 cttcctttga taaacattgt tgccggtagt gtcgccatat acatattccg gatctgggga
     6121 agaggctggc agcaattctt gtctgccttg atggtgttca ttgcatattt attcattgat
     6181 ctggcaattc acctgatctc tgaagggact tttgaacggt tgacctgggg ggttttcaga
     6241 aattacggag tagcttcaat cctcatgata gcatcctacc ctcttgtttt tttatttgaa
     6301 aaaatattcg gattcgtctc tgtatcgcga ctcagggatc tggccgatac aggtaataaa
     6361 gccctgaggg aattgtccga aaaagcaccg ggaaccatgc aacattcatt acaggtagcc
     6421 aacctggccg agaccgctgc gcggacaata ggggccgatg cgcttctttg ccgggttggt
     6481 tctctttatc atgatattgg caagataaac aacccgcagt attttgccga gaaccagaca
     6541 gccagtttca atccccattc cctgctcaat cccagggaaa gcgcccggat tgttatccag
     6601 catgtagaag acgggaaaaa cctggcaaaa aaatttggtc tacctgcgct ggtgagcgat
     6661 tttattgata cccatcacgg aaaaacacgt actgaatctt tttatacagc ctggtgcaac
     6721 gaggggggag atcctgcaaa cacagaggat tttacctatg aaggtcaatt gccaaccact
     6781 cccgaacacg tagttgtcat gcttgcagac gctgtggaag cggcctgcag aagcctgaaa
     6841 gattataccc ctgagagtat ttcggccatg gtcaacagga tggttcggct aagggtgtct
     6901 gaagaccagt tgcagaatgc cgatattaca tacagaaacc tgcgcaggat acaggaaaac
     6961 ctgaaagttc agatccagga gatgtaccat tcccgtatca cctatcctaa aagaaagcgg
     7021 ttaaaataac gacagggcgt acaccaaaac tgcaacaata aggattttca gcgcattgtc
     7081 aagggcaggc ccttcttttc tgataaccca taagatatga atccccagta aaggcaggca
     7141 aatcaggaac aaaaatgttc ctgttcccgg tttgcaggca taaaggacgg acaagaacat
     7201 cgccagtgag ataagtacca cctggtaaat cttggcagga aatgttgacc cgtttttaga
     7261 gaaccgggat aaaaatactg caaaagtgtt ttttccaaaa cgcttgtcgt tttcaaaatc
     7321 cctgatatta tttatgttca aaacggcagt tatgagcatc cctatggcag atgcgatata
     7381 caaaacggca ggatccaggg atcctgtaaa caggtaataa ctgccggcca cacctgccaa
     7441 accgaaaaaa agaaatactg caatatctcc tgctccataa taaccataag gttttttacc
     7501 ggcagaataa aagactgctg cagccagggc tgacgctccc aaaagaataa agagcaggga
     7561 cgacatctcc ctgagcgtcc caaatgccat acggatcaaa ccgaggcccg ataaaagaga
     7621 aagaacggca acaattatta tgataatgag aagttgcctt tcggtcaatc tgccactttg
     7681 aaggctcagg gatttgcgtc cggcctgatc ccggtccgta ccttttttcc agtcgcccag
     7741 ttcgtttaca aggttggaaa gactctggag cagacaggcg gtgagcaagg ccattaaaaa
     7801 caaaggccac gaaaaatggc cttgtgactt tgccaggaat gtaccggcta gtattgaagc
     7861 aaccgacaag agcagtgtgc ggggacgaat actttgtaat acgggcagca taccggttta
     7921 tttcaaaaag ttctggttca gaaaattttg aaaatctttc ttatactgat cacctaacgg
     7981 aacactggca tccttgccga atactatgcg gttttgaacg acttccgtga tttttgtcag
     8041 attcactatg aaagaacggt gtacccgcat gaaaacatcc ttgggtaatt tttcttcaat
     8101 ggatttaagg ctgaaaagcg tcatgacagg aaggctgttc tgtgaaaata tcactacata
     8161 ttccttgcgg ctttcaatat actggatatc ggagatcttt aaccttaccg ttcgggaatc
     8221 ggcccttacg aacacatatt cgttttccca tccctgggtg gctttttttt catcagattc
     8281 acttgtttct ttcttgtccg ctccagctgg cttttccttg ccggaagttg ccgccatacg
     8341 aagatcatgc tgttgcttca ccttctctgc gcatctcata aagtctgtaa gactgaatgg
     8401 ttttaaaaga tagtcggtag catccacctg gaatccttct atcgcaaaat tggcatatgc
     8461 tgtaacaaaa acgatgatag gcttatcctt gagggaacgt atgtattcca atccgtttat
     8521 gtcgggcatt tctatatctg taaaaataac atccaccttt tcattggcca tgacttccat
     8581 cgcctcggtg gggccggagc acgcttttac cagttccagg tatggtatct tacccacata
     8641 agagacaagt tgttgaaggg ctagcggctc atcgtctatt aataaacact ttatattcat
     8701 aatagttaac tatatgggta attgcaaatg tataatataa tccttttctg tctgctccat
     8761 ggtaagttta tagctgtctc catataacag attcagcctc tctttcacat ttttgattcc
     8821 cagcccgccc ggattcgtat tttttggttt gtctggaaat atggtattgg atattgaaaa
     8881 atgaaagaat cccggttctt cccagaaatt catgttgata tgggtttctt ccccgtatac
     8941 gaccccgtgt ttgaaggcat tttcgataaa aacgatcatt ataagaggtg gtaactgtat
     9001 attggggata tatctgggaa ctgttatatt gagcttcaca ttttcgccgt aacgcaacag
     9061 gttaaggtca cagtaagcct taagtactgt cagttgcttt tcgaaggata tttttgaatc
     9121 aggagtactc tcatacaaca tgtgtcgtaa aagccgggac aagctgacaa tggcctcttt
     9181 agcctttacc tcgtccgtat caaccagcac atgtatattg ttcagggtat tcatgagaaa
     9241 atgcgggctg atctggaatt gaaggaattc cattttataa ttcatcagtt tgttgtcagc
     9301 ctcatgggcc agcattttgt tcttaatgtt atcgaatatt aacctgatgg ccacattcat
     9361 ccccataagc agcgtggcaa ttacaaaatt gccgatcaaa gtggattcat atggtaataa
     9421 attcgtccat gttatgtcga atttctcatc caggatatct atgactgctt cgcaagtaaa
     9481 aactatcccc aggagcagga aaaagagcag aaaaaaataa gtgaagtaat gaccctttag
     9541 caggaacaga ggaacgtata catagtggtg aatggtcaaa cctattaaaa aaggcaacag
     9601 gatatacaag aggggctgga tttgctgctg tataaacaac ggactcagaa aagctccccc
     9661 tatgatgccc catagtgtaa ggttttccag cccgcgtagt tttttaagtt cccggcttat
     9721 atcgtattcg cgattctttg tcataagaat tagtatgcga atatacgatt tactccggat
     9781 taattaaaaa aaaggtggga tttagatctc ccacctgtaa aaagatttcg catctttttg
     9841 tgtacgtttt tagttcaaaa aaccattcat tatttatcct agttgcaaag ttaatttccc
     9901 atttcaatta atcaaagcag aattcccgtg aaacgggctt tcatcaacca aacagctttt
     9961 ttcttaaacg aacggtctga atctgtcaac ataatcttta gtagccaaat atttcctttg
    10021 tagacagtct gacaattttt ccataattgg caagctgttt catatccagg gctttaatat
    10081 cgcttaatat acagtaattt acaggcctgt tacttatcca ttcacgttga aatgccacta
    10141 catcctgcag ggtaagggat gcggcctcct caaaaacaac ctttctcata tctgtccaca
    10201 ggttcttttc cttggcctga cggtaatagt tcagtaggtt ccttcctgtt atacgctccg
    10261 tcctcaatgt agacatcagg ccctcttttg ccagggagaa tgacttttca gattccggca
    10321 tattgttaat tatttcttca aaagccttca tggcttcggc catcttatca ttttgggtgg
    10381 caataaaact ggtgaaataa taagggaagc cttcttcgtt ccgggcaggt tcgacaaacc
    10441 gtgcgtttgc gctgtatgcc agccccctgg cttctctcat ttcctggaaa acaatagcgt
    10501 tcatggaaga accaaaatat tcgttataca tcctggcaac agaataggga acattggcat
    10561 acgtttctcc ctctctggca gaatacgttg cgtaatacat ctggttggcg tcatactgag
    10621 ccaggtaaac ggtattttct ttggttatct gtcgttggaa gtatttcttt tccggtgctg
    10681 gaacaagagc ctcttccatg gggaatgagg tggttatgac tgctcctgcc tggtccatgg
    10741 tggcgggtcc gaaataataa acatctgctt cgtacttcaa cagatcctgt attctctgga
    10801 tcaataatgt cgggtctgtt cttttaagtt cattatccgg aactatgttg gtagaggggt
    10861 tgatgggacc gtactgcaca taattctgca ggcgctggaa attggtcctc tgatctccct
    10921 tggcgtcgga acggcttttc aggatattgg cggtcagtgt cttgtataca tccggatctg
    10981 cctgtgcacc ggtaatcaat tctattagaa gttccagggc gggttgcatg ttctcggaca
    11041 atccgttcag ggtgatggtg catgtattgt ccgacacata ggtactgaat gtacaggcca
    11101 gttcataaaa tgcagtactg atttggtcgg ctgttttatc ggcagtaccc aggtaattca
    11161 gatagtcaaa tgcaacagga agtgccctgt cattgatgct tccaatctca tagacaaagg
    11221 ttaaggtaaa caggtcgttc agtgtattgg gcacgtatat gaagtttatt ccctcccgca
    11281 gcgtgtcttt catataatcc ttttcaaaat caacaaacac aggttctatg ggctccacca
    11341 cagaggacac gatttctttc aggtaagcag aagaggtatc tctgttggtg aaaatcgggg
    11401 tgatcttggg tttctcgatt ttcttataag atgtgtcggg gccttgcctt ttataaatca
    11461 cggcataatt gtcgtcctta aaatactggt tggtaaaagc caccaggtct tctttggtga
    11521 cagcttccat ccggtcaata aaacggacat tctgttccca gggtataccg ttgataaagg
    11581 cactgacaaa catattggcg cgtccgcggt tgctttccag tctgcgcatc atattgaatt
    11641 tgtaattgtt gacaattgcc ttgagcattg attcttcaaa atcgccgcgc cttaccttgt
    11701 caaggcatcc cagcatcagt tcttttacct ggtccaggct ctgcccttgt ttgggatttc
    11761 cctgcaacac cagcgtaccc ctgtcagaga gcatgtaggc gaaagctcca gactgcaata
    11821 ctttttgctg ctgattcagg tccaggtcca tgatgccggc tctgccattg ttgagcactt
    11881 ctcccaaaac ggtaaaaagc gcatcttcca ttgaagctgc atggggcccc tgccaaccca
    11941 gggtgacgta ttccgattcc agtccccata cttctacagt gagcggccca ttaacgggct
    12001 cttcctgcgt aaggacatgc tcaggaagat tgggattggg ttcccacgaa ccgaaatatt
    12061 catcaataac ggctatcatt tcatccggat caaaatctcc tgacaggcaa atggcgatat
    12121 tgttgggaac ataatacgtc gtgtaataat tctttatatt ggtgatagac ggattcttca
    12181 ggtgctcctg cgttcctatg acggtttgtg tcccgtaagg atgagaaggg aaaagtgagg
    12241 caaacagctt atcgatgact ttttcgctgt ccatggtcga atacatgttc atttcttcat
    12301 ataccgtttc cagttcggta tggaaaccac ggatcacatt gtccgaaaaa cgctctgcct
    12361 ggatctttgc ccaattcttg atctgattgg agggaatatc ctctatatag accgtctgat
    12421 cgtaacttgt accggcattt gtccctgttg acccgatggc agacataagc ttgtcgtatt
    12481 cattgggaat ggaatagcgg gaagcctcca gagaaatgct gtctatgcgc ttgtaaatgg
    12541 ctttacgttg ctccgggtca gcggttgtcc tgtattcctc aaagaggagt tcaacctgat
    12601 ccagaagggg tttttccagc tcgtagttct gggttccgaa cttactggtg cccttgaaca
    12661 taagatgctc aaaataatgg gcaagacccg ttgtctctga cggatcgttc tttgacccga
    12721 ccctgaccgc aatataagtc tggatctttg ggatttcctt gttgactgtc atatagacct
    12781 ttagaccatt gtccagggta tatatgcgtg cattgagcgg atcaccttct acagcctgat
    12841 agctgtaacg gggtccgcat gataatatga caccgaatgc gatcagtgtc aaaacggcag
    12901 ttgtgaattt gtgtaatcgt gacataatca tagttttatt ctcccaaagg tacaaatatt
    12961 tgtctgcggc actgtatttg ttgaattatt agataaaaaa atcgatcatg caagctacat
    13021 cacctctatc catgtactgg atcattttaa tcggaacaat gctcctcagc tggcttgttc
    13081 aggcaatact caactcgaaa ttcaccaaat attcaaaaat tgccatatct tccggcatca
    13141 cgggagctga ggtagccagg aaaatgcttc aggaaaacgg gatcaccaat gtgctggtaa
    13201 cttcaacccg tggcaggctg actgaccatt acaatcctgt gacccggacg gttaacctga
    13261 gcgaaggcgt atacaacagc aaaagtatca tggctgctgc ggttgctgcc catgaaagcg
    13321 gtcacgcctt acaacatgcc cagggatacg ctcctttgaa aatgcgctct gcccttgttc
    13381 ccatagtaaa ttttgcttca cagtgggtta tgtgggtcat tgttgccggc atgctggtca
    13441 tcaacacgtt cccgcaattg atgcttgcag gtatcatctt gtttgccctt accacgttct
    13501 ttagccttat caccctgccg gtagaggtga atgccagcca aagggcactg gcatggctca
    13561 ataatacaag gataaccaat tacgaaacat atcccatggc caaggatgcg ctgaaatcgg
    13621 ccgcttatac ttatttcata gcagccatca gttctctggc cactttattc tattatatca
    13681 ccctcttttc caggaggcgt taagacttct ctttccgttt tttgtatatt tgtcatgatt
    13741 caaataataa gttcatggca aaagttgtca catttggaga aatcatgctc aggctggctg
    13801 taccccatca tcttcgcatg ggtcagtcag acaggttcaa tgccactttc ggtggcggag
    13861 aagccaatgt cgctgtatct ctttccaatt tttccatcac cgcatcattt gtgaccaggc
    13921 cttccggata atgacctggc agcggcctgt ctgaaagaat tacggtcatt cggggtggat
    13981 gtatctgatg tgataactgg tggagggcgt atgggattgt attacctgga aacgggagct
    14041 gttgccaggc cctccaaagt gatctatgac cgggaacatt cttcctttgc cgaaatcaaa
    14101 cccggtatga tccgatggga aaaagcactg aaagacgcac aatggttcca ctggactggt
    14161 atcacaccag ctgtaagcca cggggcagcc ctcgcttgta tggaaagagt ggaagttgca
    14221 aaaagaatgg gtataaccgt atcgtgtgat ctgaattttc gtaaaaacct ctggaactac
    14281 ggcaaacagg cttcgcaggt aatgcctgaa ctggtagaac aatgcgatgc aatcccggga
    14341 aatgaagaag atgccgaaaa gattttcaat atcaggccaa aaggatatag cgccactgcc
    14401 ggtatggcaa aaccatgcat actgccccgg tttatgacat cacccatata atagaccatg
    14461 ttggcggcgg cgattccttt gccggcggat tgatatatgg attgctcaac tggccgggtg
    14521 accacggcaa ggcgcttcgt tttgccaccg ccgccttgtg tctgaaacat accatttatg
    14581 gcgattttaa ccgggttgct gtcaaagaag tggaaaatct tatggaggga gacgcctcgg
    14641 gacgtgtaaa cagataaatt aattcatcat ggcaagattc ggcaaatggg aggtaatgga
    14701 aacaataatg ggaacaggtt tcatccctgt tttttaccat ccccggatac tgacagcaga
    14761 aaaggcattg aacgcctgtt acaaggcagg tgttcgctta tttgaattca cgaaccgggg
    14821 ggattttgcg cacgatgtat ttgcctccct tgagaaattt gcggcagaaa aatgtcctgg
    14881 cttgatcctg ggagccggca ctatttcaga cccggcaacc gctgccctgt tcatacagat
    14941 gggagccaat tttattgtcg gtccgaattt caatcccgcc gttgccgctt tgtgcaacag
    15001 aaggctggtt ccatacatac caggttgcgc tacagttaca gaagcaggca atgcacagga
    15061 agccggatgt gatatttgta agttattccc tgcagaccag ctgggcggcc cctcatttgt
    15121 aaaagcctga aagctcccat gccccggagc ctcttcctta tcagcggagg ggtaaagccg
    15181 gaagaaatca atatcggtga atggatcaaa gccggagccg aatgtgtggg catgggctct
    15241 gcactcttta ccagtgagct catgaatgca gaagactggg atggtatatc cgagctgtgt
    15301 tcatgctctt tcaaagcgat agcacgggaa aaagctttac aatgatcttg ctgtagcggg
    15361 gaaccagcgt tttcataatt acccatccca caagataggc agccgaataa atgcagaata
    15421 caataaagta tcctgccggt tcgcccttga atccaagaaa tgtcatctga gtgcgggccg
    15481 catgatcgag caggactcct gaaaaacgat tgatgacata agaagatatc atcccggccg
    15541 ttgtgcctat tccggtaatg gtagctatag tgctttttgg aaacatatcc cctatggtag
    15601 aatataaatt ggccgaccac gcctggtgtg ccgcccccgc gattcctatt attatgaccg
    15661 ggatccagta agaaatatta ccagggggct gggcaaacat tgtcagtaaa aggaaaaatg
    15721 caaatataag catggccctc atgcgtcccg aatacggttc catgcctttc ctgtctacaa
    15781 agtacgtagg cagccacacc ctccaattat tgaaagaaga gtaatagaat agaggacaaa
    15841 tatcagcaat tgggcggtag gattattgga tggcaggtca tagactgaac ttaggtaggc
    15901 cggtgtccag aacaggaaaa accaccatac cccgtctgtc atgaattatc ctactgcaaa
    15961 cgctcaggtt tgcctgaaag tcaaacattt ccagaaagat atcctatccg gaccctttgc
    16021 cgggatttca attgcaggta catcctcccc atcctgctgt atgtacatca actcggcctt
    16081 gttcactttg aggtgaactt ccggtttttt gtaaacaaaa atccaaaaac ccatccaaat
    16141 aaatccaagg gaaccaatgg ccagaaaagc tgcctcccag ccaaaccggg tagcgatcag
    16201 cgacactgtg acaggagcca caagtgcacc aaccgttgcc cctgcattga aaatgcttgt
    16261 ggcaaatccc ctgtcctttt tcggaaagaa ttccgcagta gccttaacgg cagccgggaa
    16321 gtttcctgcc tcccccccat ggccagaatg ctacgggcaa acatgaaaag cgatacgctt
    16381 accgtggtga ttcgggccac cacatccact ccctgtgcca tgcgaagcgt ctgggcgcta
    16441 tccaggccca caatatcctg tgtaatcaca ctgcataagg catgcacgca ggctccggca
    16501 gaccatacaa ggatagccca taaatatcct ttctttgtgc ccagcctgtc tactatcatt
    16561 cctgcaaaca aaactccagt ggcatacatg attgagaaga ttcctgtttt cttcccataa
    16621 tgcacattgg tccagtgaaa ttcgggtgca ataaaatcca tccaggtaag tgacagcacc
    16681 tggcggtcca ggtaattgat ggtagtggcg aaaaaaagca tggtacagat ggttcacctg
    16741 taccgggtca ttttttctcc tgccggcttc attttcatat tcagaaatta aaataatcac
    16801 gagcgttgta ataacagaca gcctggacaa tatgcctgcc tatatagtca atttcacctg
    16861 caggaagcaa tccctgttcc atgtcgtttc ctaatagatt acacaaagtc cttcggaaat
    16921 aatcatgacg tgcatagctt aaaaaactgc gtgaatcggt aagcatgccc acaaaccggc
    16981 taagtagccc cagcagggac aaggcgttga gttgcttttc catcccgtcc ttctggtcca
    17041 ggaaccacca agcagctcca aactggatct ttccgggaat ctttccgtcc tgaaaattac
    17101 ctgccatagt ggctaccatt tcgttatccc ggggattcag gttgtagatg atggttttgg
    17161 tcagtttatc acggctgtcc agcatatcca gaaagcgtta caggtttttt gctgtcgtaa
    17221 gatcgcctat tgaatcatac ccggtatcgg ggcctatagt actgaacata cgggaattgt
    17281 tatttcgaag ggctccataa tggtactgct gggcccatcc cttttcccaa tccatgatcc
    17341 cgagctccac cagcatggcc gttttgaatt ggcatatctc tttttcatcc ggctgcaagc
    17401 catctattac acgatgaaaa atagtgtcta tagcagcatc tgtgtaatcc cgggcataaa
    17461 actcctccag accgtgaacg gaaagacggc atcccatggc atgaaagaaa tcatggcgct
    17521 tgagcaatgc ctaaatcagg gtttggtatg aatgaattgt tattccagag actccagaaa
    17581 gtttctccac atacttcctg tactttggag gatcctcgac ggccatggct ttgtcaggac
    17641 gccaggccgg cagcactttg acagggaagc cgtcccgaaa cagctttgca tgatattcta
    17701 gagaatcggc cggattgtct gtagtgcaga gcgtctccac tccaaatttc tgaatgagtt
    17761 catgatactt cgcttaagcc catatggatt cccttagtat ctggtccaga tcatgcgtga
    17821 ggataaaact gaccagcctt gtcaaatgcc acacccccag aataatggca acagaccagg
    17881 tcacccgggg attgtatagc catctgaagg aataatcctt tccccgtatc aacccggcag
    17941 tcatcacaac cagggcacag gcaaaaaaca gaacaagtac cggtccaaaa agatggaatt
    18001 ccaggctttc tatccattct cccctataga gggataccat ggatttgacc atgccacagc
    18061 ccggacacgg cagtcccgta agcattttga aaggacaaaa tgactgcctg gcctgcaaat
    18121 tggctgcccc gggatcaaaa agcaagatga ccaggggcat gagcaaaaat aggagcaggg
    18181 ccgttatggc taagattttt cgcctgcttc tcacaatggg tttatcggta tagtttattg
    18241 atctcgcttt gtacgatcat ggctgcaatc atgggaaaga aaaaccctag caacatcagt
    18301 aaaatggact ggtctctttc gggtattccc agcctttggt ccagcgctgg catgccatcc
    18361 cggcctacaa taaagaaata atacatattg accggccata tgcaaccggc aaaaactgcc
    18421 acaacaggtg acagtatctc cctcccgctc agggcattaa aaactttggc ggcctgcagg
    18481 ttccagtata taaggtacag gccgcatgtg acgaatccta acaggagtac caccaggggt
    18541 tctcttttaa attcgggaat gttatcaata atctgcataa tcaattgtat tagtcaataa
    18601 ttaatccgta ttctgtctca ataattttat ttcgccggtt ttccctgcgt actttcctgt
    18661 taaactccca tgaggagtga tgaatataat cacgtggatg atccaggtgc agcgtaacgg
    18721 cagaatatct gatctgcctc ccccttatgc ccagccgctc cagcctttcc ccgaattccc
    18781 tgtcttctcc tccgtagcga agcctctggt caaatcccct
//
LOCUS       DAOSOF010000022        18808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_2947_length_18808_cov_4.883005, whole genome shotgun sequence.
ACCESSION   DAOSOF010000022 DAOSOF010000000
VERSION     DAOSOF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_18808_cov_4.883005"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLM68_02330"
     CDS             <1..610
                     /locus_tag="PLM68_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT54236.1"
                     /translation="QLALDNNPDMISYERQLIEAQKNVAQQRANRGFQANLNVAFGLN
                     QQATGLLDSYKDPRDMQTVSIGLTVPILDWGRGKGLVKMALSNEELVRRQVEQSRLDF
                     IQDINLNVRQFNMQEQQFFIAAKADTIAQKRYDVAKQRYLIDKITVTDMNNAQLDRDQ
                     ARVGYVQALFNYWRYYYELRSITQYDFINNRRLDADFDSIVD"
     gene            complement(626..1744)
                     /locus_tag="PLM68_02335"
     CDS             complement(626..1744)
                     /locus_tag="PLM68_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54237.1"
                     /translation="MVTVKEITSGSDKRKFFAFPVKLYKDNPWFVPTLISEEMRDFNP
                     QKNAAFEYASCKMFLAYRDGEIVGRIAAILNNAYNYKMNVRQLRFTRFDFIDDYKVSA
                     ALLEKVREWAMFLDMKEIVGPLGFSDMDKMGMLIEGFEEMDMYITTYNYQYYIEHMER
                     LGLQKKVDWLEFQVKLPERMDPELERLALSAMERYGYSVRKFNKVKDLKPVFREALPL
                     MNEAFSELFGVVRISEKQLMDFANLLMSICKLDYITVVQNKAGQIIGYGLMAPSISRG
                     VRLGKGRLFPLGLLGIMRDIRQSRVLDMYHIGVWPTYQNRGVNAIIIYNSIINAMKTG
                     VEYAETGPELEDNIKIQSQWKLFEHRQHRRRRCYTMNV"
     gene            1811..2377
                     /gene="efp"
                     /locus_tag="PLM68_02340"
     CDS             1811..2377
                     /gene="efp"
                     /locus_tag="PLM68_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT54238.1"
                     /translation="MATTADFKNGMYISFNGKPCSIIWFQHVKPGKGGAFVKTKLRNL
                     ENGRVLENTFNAGEKVETIRVERRPYQFLYKDDMGYNFMHTETFEQISLQPDLVENSD
                     LMKEGQYVEMMFLADEERVLTCELPPFVEMEITYTEPAVKGDTASTNALKAATLETGA
                     QIMVPLFINQGERIKVDTRTRGYSERVK"
     gene            2384..3826
                     /locus_tag="PLM68_02345"
     CDS             2384..3826
                     /locus_tag="PLM68_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT54239.1"
                     /translation="MRRWPAMLAFLVLLSCHTGVETPDPATITDLIARGNFEEAEKKI
                     LLYTSTSSMTAEERLHWSWETERIHRISLDFNKTEEEALEYIRQYFPKVTPQQMSDWE
                     TSRALECMVINGEKRYFSRAFRNLFRIDSLCLARYEEINGPAIDAKAPLLEEHLPRIV
                     ELTGKTGAVIVEPRTFTITYTLTVPANTVPEGEILCVWMPLPRTDVPRQNGFRLLSVS
                     EPDYIISPDSYIHKSIYMEKPAVKDQDAVFTYSFTYTARAQYHHIDAIKDVLPYDTLS
                     PEWKEYTKQELPHIITTGPVAELAEKVVGDETNPFLRLQKIFTYISQTYPWAGAREYS
                     TIESIPLYVLENGHGDCGQVSLLFISMCRSLGIPARWESGWMLHPGEINLHDWAEAYL
                     EGIGWIPVDQSFGLQQGPFPLFYSSGMDSYRMAVNRGTGGDFFPAKIYPRSETVDFQR
                     GEVEWRGGNLYFNQWDYQLILHPFDQQGIQ"
     gene            complement(3820..5052)
                     /locus_tag="PLM68_02350"
     CDS             complement(3820..5052)
                     /locus_tag="PLM68_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT54240.1"
                     /translation="MVTTLTSGMQCAVLTTTSPVSYCALSTLAGTRFEPSGFAGLAHF
                     CEHMLFKGTLRRKSYHINNRLERLGGEINAFTTKEELVLHATVLREDLGKALELMSDL
                     VFHSVFPESEVTKERDVILDEIRSYKDSPMDLIYDSFEEKLFSGTSMATPILGIPAEL
                     KKITSRHMKQYVGERFLPGTMAVSIVGNVAPKKALLLAENYFGTGAFSQKSTAAAGLP
                     ANHYPLENSNRFDMAEKRGTYQTHCLTGAAAYGLHHKKRVPLVLLMNMIGGPASNSRL
                     NMLLREKYGLVYTVEASYAPYMDTGFAGIYFGTDRPNLDRCRELTEKVISRYAKDSLT
                     TLQLDRAKKQLAGQMSVTEDNHEVQCLSMGKSLLSFGKVISHREMMSLIDSVTPSQIL
                     ESAGEIWDTSRRSTLVYY"
     gene            5126..6841
                     /gene="dnaX"
                     /locus_tag="PLM68_02355"
     CDS             5126..6841
                     /gene="dnaX"
                     /locus_tag="PLM68_02355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT54241.1"
                     /translation="MDPFVVSARKYRPQQFNDVVDQDNIVTTLKNAIQRKQLAHAYLF
                     CGPRGVGKTTCARIFARTINCLEPGPGMEACGTCESCVSFAEGRSYSIHELDAASNNS
                     VDDIRELIDKVRIPPQVGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPSYALFILATTE
                     KHKVLPTILSRCQVFDFNRISIKAMTRHLAEVALKEGVTIGQDSLQVIAQKADGAMRD
                     ALTLLDQCIAVSGKEVTYQQVISNLNVLDREYYFRLTSMALEGRFDTMLLLFDEVLAR
                     GFNALHFIGGLSSHFRNLLVCKQPESLPLLEYGEETARKYNEQAAACSLPFLFGALGI
                     CTQAEAGYKQSNQPRLHIEMALVRLSRVHNQETPKPRLNDMTTDTGPLDNAPGPAAIP
                     FRASTPGPTAIQPPAPVPVPAPTPEPAITTSINRLKHQNKKEKQREEEPSGETTGSGE
                     LTMEALEKALPGLMNLFKDKPNLSATLSRCPPLIVDKCKVQFTVVNDLQKTWIEEHYG
                     RIIDTYLRNTLRNKEVCMFLVVEEAPGNIAVNTCYMPEEKAKYIAEQHPEVAALRDDL
                     NLDIS"
     gene            6854..7699
                     /gene="lptB"
                     /locus_tag="PLM68_02360"
     CDS             6854..7699
                     /gene="lptB"
                     /locus_tag="PLM68_02360"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOT54242.1"
                     /translation="MRLHCHNLVKRYGLRTVVKNVSIHADQGEIVGLLGPNGAGKTTS
                     FYMITGLIKPNSGKIFLDNEEITTYPMYQRAKKGIGYLAQEASVFRKLSVEDNIMSVM
                     ELSTFTKAERKERMERLIAEFGLQTVRKSYGIQLSGGERRRCEIARALALDPKFILLD
                     EPFAGVDPIAVEDIQQIVAQLKTKDIGVFITDHNVHETLAITDRAYLLYEGSILESGT
                     AEELASNSEVRHKYLGQNFELRKIVPVGTNDISETDNKEYDPGEYLPDGDYSEDAVSR
                     EKGLN"
     gene            complement(7692..8969)
                     /locus_tag="PLM68_02365"
     CDS             complement(7692..8969)
                     /locus_tag="PLM68_02365"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT54243.1"
                     /translation="MKKIIFLLCCLVFTCCAGSGEENVELQESSDKIILEGEIAGYCE
                     LFSLPLMDYSKESDVIRKNENLAGILQRRGVDFQRVFLLTEKARGVFDLRKLKAGNPY
                     RLLYAPGVSQPAYMIYEENFRTHICFSLTDSLWVTRAEIPLEIEDKFVDITISTSLWQ
                     DLEDAGYNPLVANGLSEVFAWTVDFFGLQKGDTFRASYQAYMLEGKEVEVGTILAASF
                     IRSGRIQMAFRFIQDSLPGYWDQDGVNLQRAFLKAPLKYSRVSSGFSYSRRHPVTRTL
                     RPHTGIDYAAAVGTPVVSIGEGTVIERNYTRGGGNTVKIRHNSVYTTAYLHLSRFARG
                     LVVGKRVAQGEVIGSVGSTGVSTGPHLDFRVWRNGKPINPLTMESPPADPIHKSDMEN
                     FLVHIAPYSRRLEPDFYKKVALQIIGFTGIRFN"
     gene            9098..9547
                     /locus_tag="PLM68_02370"
     CDS             9098..9547
                     /locus_tag="PLM68_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54244.1"
                     /translation="MAKFIGEYNAKLDDKGRLVFPSALKALTDPGIPLRFVVKKDLFS
                     PCLEMYSYEEWEKQSEAVKARLNPSFNREHAVLWRAYMSNRAVVEPDEKTGRILIPRH
                     LLNMIGVNKEVIFAGADFKIELWPKEAYQVSLISNDDYVSLAEKILS"
     gene            9559..10443
                     /gene="rsmH"
                     /locus_tag="PLM68_02375"
     CDS             9559..10443
                     /gene="rsmH"
                     /locus_tag="PLM68_02375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT54245.1"
                     /translation="MVFYHIPVLLRESIESLAIRPQGAYVDATFGGGGHSRAILEQLG
                     PEGRLLAFDQDSDSRNNMPDDKRISWIHSNFRFIHSVCKYHGMHQADGILADLGVSWH
                     QFDTEERGFSFRFDALLDMRMNRSSEKTAANVVNEYAREDLASLFSRYGEIQNAGALA
                     GAIVKERSKHPLNTSGDLCKALKRFMPRNAEHKYLAKVYQALRMEVNDEMRALEGLLH
                     QGLALLRPGGRLVIITYHSLEDRMVKQFFRDKTEAGLIRLVNKKPIVPQEREISENTR
                     ARSAKLRAAEKCGEEEHG"
     gene            10436..10768
                     /locus_tag="PLM68_02380"
     CDS             10436..10768
                     /locus_tag="PLM68_02380"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOT54246.1"
                     /translation="MAKSKGEKIVSTLFGGRALLSGVLSRHLNFILYIFFLLILYISI
                     NNAVEKTLLANYQVEKAIKLLHADYTRKSADFMKLSQQQEIERELRNRGSELMAPVNP
                     PAWIKSER"
     gene            10765..12540
                     /locus_tag="PLM68_02385"
     CDS             10765..12540
                     /locus_tag="PLM68_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT54247.1"
                     /translation="MNTSAKMMIVYIGYLLVAMLAIGQTVHLQFFSDYKDQLDAIVFR
                     NIEVQAYRGSILDCDGRVLATTVPFYEVRMDCSIPDDLMLNRHLGDLSVALSRFFGNK
                     SAAAYENEIIQARVGKKPGYRNLLLGNRLVSYDELQKIKQFPIFSQGQFRGGLIANQK
                     EKRIYPYGRLAYRTIGYISEDEQTGVGIEYAYNSYLKGNPGKERVRRRSGDDKWVPVS
                     SQPEVAPKDGLDIVTTIDIDIQGAAESALREQLSVDSTFEGATTVVMEVGTGAIRAIV
                     NMKRMSDGTYDETFNYAISESTNPGSTFKLATLIALLEDGYVRLTDSIATGRNGWNYY
                     GKTFTEATSSYGTITVQQAFEKSSNVAFAKLAVEKYGNREKQFINRLYNMKLNERLNI
                     ELIGEGRAQIPFPGEGGWSRLSLPMIAMGYEMMLTPLHVLTLYNAIANNGRMVTPYFI
                     ERFMQHGRLVEQSERTLLSGTLCTSATLKAVHQALRGVVENGTASRINDPRYNISGKT
                     GTAQIAYDGAYVHNGYRKHQASFVGFFPSEDPKYSMIVILYSSPTRGNFYGASWAAPV
                     FKKIADHIYITNPFWNAPLGVTEKT"
     gene            12553..14028
                     /locus_tag="PLM68_02390"
     CDS             12553..14028
                     /locus_tag="PLM68_02390"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT54248.1"
                     /translation="MELVKLIEVLVTGRITGSTAREITCISQDSRKVSEGCLFVAVQG
                     AQTDGHLFISQASRNGAAAVVYQKGCLASEQEMEALSGNTTFIEVGDSQLALAMLADA
                     WYGHPSANMILTGVTGTNGKTTTATLLYRMFGQLGHKCGLISTIAYYIGQEEIPATNT
                     TPDALTINSLLDQMLRRGCTHCFMEVSSHSVVQHRITGLHFAGGIFTNLTHDHLDYHK
                     TFAAYRDAKKRFFDMLPAGAFALVNEDDRNGKIMVQNCPARTYTYACRKMADYNARIV
                     EHTVDGMQLMIDGVEVWSSFIGMHNAYNLLAVYACAAILKEPREETLRILSGLTTVRG
                     RMEYVRGGENLTAVVDYSHTPDALKNVLLTLRELVRPGQELVCVVGCGGNRDKTKRPL
                     MARIADQYADLCIFTSDNPRFEDPEDILDQMIAGLTPEQQDRHLRITDRRQAIRTAIK
                     MARPGAVVLVAGKGHETYQDIKGVRNHFDDKEILTEILKTG"
     gene            14030..15394
                     /locus_tag="PLM68_02395"
     CDS             14030..15394
                     /locus_tag="PLM68_02395"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT54249.1"
                     /translation="MLYHLFEYLKENVDFPGVGLFRYISVRSIMSVIIALFVSTIAGK
                     YIIARLKRNQITDTSVKTGENGKSGTPTMGGIVIIVSLLVSVFLLCDLSNLYVQLLVI
                     STLWLSTIGFLDDLLKLRHMAGKEDSRVVRFLSRLRRKDKDGMKGKLKVYGQVGLGLI
                     VGIALYVSDKAVIVEMKNVPSDTDQTEQVETTSYVRHVKSTKTTIPFLKDNLFDYSWL
                     TPFKGAAGQKAGWIVYILVAIFIVTAVSNGTNLTDGRDGLATGTVAIVGVTLGVLAYL
                     TGNIVYARYLNIMYIPNSGEMVVFMAGLIGALIGFLWYNTYPAQVFMGDVGSLTLGGI
                     VAVFALMIRVELLLVILCGIFLAESFSVMIQRFWYKYTRIRATKKLMAQGKYHPGDVV
                     KGERVWVRAPLHDHYIYDRKKVDTPILLKTPREPIAEPKVVVRFWIISILLAVLTIIT
                     LKIR"
     gene            15408..16745
                     /gene="murD"
                     /locus_tag="PLM68_02400"
     CDS             15408..16745
                     /gene="murD"
                     /locus_tag="PLM68_02400"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT54250.1"
                     /translation="MGNRLVILGGGESGVGAAVLAKKQGYDVFLSDSGVIQPGARALL
                     DEHKVPFEEGMHTRSLIMTADQVVKSPGIPDTVPVVEDLNKAGIPVISEIEFAGRYTN
                     ARKICVTGSNGKTTTASLIYYLLRNAGLNAGLAGNVGQSFAYQVATQNYDIYVLELSS
                     FQLEGMYDFKADIAILLNITPDHLDRYHHDLNEYVRAKFRITQNMDQDDCFIFCRDDE
                     ISMDHLSRIVLKAKMLPISQQVVIPQGAYTSDNRMHVTYGEEEFEIFLNELALQGKHN
                     IYNSMAAALSAKVMHISNPVIRESLTSFKSIPHRMEQVARVKGALYINDSKATNVNSV
                     WYAIESLKTPVVWIAGGKDKGNDYTPLYDLVSEKVKALICLGADNKKLHDCFEGRVPC
                     IIETNSMKDAVNQAYALAQSGDTVLLSPACASFDLFKNYEDRGDKFREAVRGL"
     gene            16754..17980
                     /locus_tag="PLM68_02405"
     CDS             16754..17980
                     /locus_tag="PLM68_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT54251.1"
                     /translation="MNKTGKIRLKGDTSLWALVIIFSLLSLVFVYSSSSRIAAMQNTS
                     TFSIMIEQMGHILIGFGIIYLFHIIPLKTYRILAYFGYIVFLVLLLLTMFLGVTRNEG
                     TRWLSIFGFSFQTSEFAKIALILFVAKVLEDDKLDSFKDFFLRLFLPLLAFILPIIWE
                     GFSTGVILSLTVLIMLLISQVRFSHIAKTAGIVVLLAGLTYGGMILNRTLRKKTDPSL
                     VSTSRLETVEKRVSSFLGFGQAEEEKENDQVMYAKVAIATGGVGGKIPGNGALRHVLP
                     LSNSDYIYSIIVEETGLIGGIFVIGLYFWLLFSVIIITKKCTKIFSSMLVSGLGVLIV
                     TQAMIHILVNVGLLPVTGQTLPMISSGGSSVLAVSAAFGMMLSVSRALQDEQLDSEDP
                     AKEETNKAITHENPQS"
     gene            17961..>18808
                     /locus_tag="PLM68_02410"
     CDS             17961..>18808
                     /locus_tag="PLM68_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT54252.1"
                     /translation="MKTRNHNRIIISGGGTGGHIFPAISIANAIRMKDPEAEILFVGA
                     LGRMEMEKVPAAGYKITGLPIAGLRRDKSVSGVMANLMLPVRLLKSVLAAGRIVKDFN
                     PTVAVGVGGYASGPLLWIARKKKIPYILQEQNSFAGKTNKMLARGAAYICVAYSGMEQ
                     FFPADRIVLTGNPIREGIHPATAQEKKEALLHFGLDPGKKTLLVTGGSLGAGTLNRCV
                     REYLPRTREHPVQILWQCGKHGMRLAAESLKEYPGVPVHAHEFIARMDLAYAAADLVV
                     SRAGAG"
BASE COUNT         5054 a         4597 c         4733 g         4424 t
ORIGIN      
        1 gcagctggct ttggataaca atcccgatat gatctcgtat gagagacagc tgatcgaagc
       61 ccagaagaat gttgcccaac aacgtgccaa ccggggattc caggccaacc tgaatgtcgc
      121 tttcggtctg aaccagcagg caacagggct gctagattcc tataaagatc ccagggacat
      181 gcagacagtc agcataggac tgacggtccc catacttgac tgggggaggg gcaagggtct
      241 tgtgaaaatg gccctgagca acgaagaact ggtacggcgc caagtggagc agtcaaggct
      301 ggatttcata caggatataa acctcaatgt ccgtcaattc aacatgcagg agcagcagtt
      361 ctttattgcg gccaaagcag acaccattgc acagaaacga tatgacgtgg ccaaacagcg
      421 ctacctgata gacaagataa cggtcacaga catgaataat gcccagcttg acagggatca
      481 ggcacgggtt ggatatgtcc aggccttgtt caactattgg cgatactatt acgagctcag
      541 gagtattacc cagtacgatt ttatcaacaa caggcggttg gatgcggatt ttgattccat
      601 cgtagattag gaaccgtttg tcagattata cgttcatggt gtagcacctt ctgcggcggt
      661 gctgccggtg ttcaaacagt ttccactggc tctggatctt gatgttgtct tccagctcgg
      721 gaccggtttc agcatactcc acaccggttt tcatggcgtt gattatgctg ttgtatatga
      781 taatggcgtt tactcccctg ttctggtaag tgggccatac ccctatgtga tacatatcca
      841 gcacgcgaga ctgtctgata tcccgcataa tgcctaacaa acccaagggg aagagcctgc
      901 ctttgccaag acgcacaccc cggctgatag aaggagccat caggccgtag cctatgatct
      961 gaccggcctt gttctggaca acggttatgt aatccagttt gcatatggac atgagcaggt
     1021 tggcaaaatc catcagctgt ttttcagaaa tgcgaaccac gccgaacaat tccgaaaatg
     1081 cctcattcat gagaggaaga gcttccctga aaaccggttt caggtccttc accttgttga
     1141 acttccgaac ggaatagccg taccgctcca ttgcactcag ggcaagccgt tccagctcag
     1201 gatccattcg ttcaggaagc ttaacctgga attccagcca gtccaccttc ttttgaaggc
     1261 ccaaccgttc catatgttct atataatatt gataattgta ggtggttata tacatgtcca
     1321 tttcttcgaa tccttctatg agcataccca tcttgtccat gtcggaaaag cccagcggac
     1381 cgactatctc tttcatgtca aggaacatag cccattccct gactttctcc aggagcgcag
     1441 cagagacttt ataatcgtct atgaaatcaa aacgggtaaa acgaagttgg cgcacattca
     1501 tcttataatt gtaggcgttg ttgagaatgg ccgctattct gcctacgatc tccccgtccc
     1561 tgtatgccag gaacatcttg caggatgcat attcaaaggc agcgttcttt tggggattga
     1621 aatccctcat ttcctctgat atcaatgtcg gaacaaacca cggattatcc ttatataatt
     1681 tgacaggaaa tgcaaagaat tttcttttat ccgatcctga agtgatttct ttaactgtaa
     1741 ccataaggga aacaaattta aaaaaatcac ataaatttgc gacatatatc aattttcata
     1801 ctcatttttt atggcaacta cagcagactt taaaaacgga atgtatattt ctttcaacgg
     1861 aaaaccctgt tccataatat ggttccagca tgtgaagccg ggtaagggcg gtgcgtttgt
     1921 aaagaccaaa cttcgcaacc tggaaaacgg acgggttctg gagaacacct tcaacgccgg
     1981 agaaaaggtg gaaaccatcc gtgtagaaag gcgtccatac cagttcctat ataaagacga
     2041 tatgggatac aatttcatgc acacagaaac ctttgaacag atatccctgc aaccggacct
     2101 ggttgagaat tccgacctga tgaaagaagg acaatatgtg gaaatgatgt tcctggccga
     2161 tgaagaacgt gtgctgacct gtgaattgcc tccctttgtg gaaatggaga ttacctatac
     2221 agaaccggct gtcaaaggtg atacggcatc taccaacgcc cttaaggctg ccaccctgga
     2281 aacaggtgcc cagatcatgg tccccctgtt catcaaccag ggagaacgta tcaaagtgga
     2341 cacccgcacc cgtggttatt cagaaagggt aaaatgatcc ggtatgagaa gatggcctgc
     2401 catgcttgcg tttctggtcc ttctttcctg tcatacagga gttgagacac cagatccagc
     2461 cacaataacc gacctgatag cccggggcaa ctttgaagaa gcagaaaaaa agatcctgct
     2521 ttatacatca acgtcatcca tgactgcaga agaaagactc cactggtcat gggagacaga
     2581 acgcatacac aggatcagtc ttgatttcaa caagacagaa gaagaagccc ttgaatacat
     2641 ccggcagtac tttccgaagg taaccccgca acaaatgtca gactgggaaa catcccgcgc
     2701 gttggaatgc atggtgataa acggggagaa acgttatttc tccagggctt tcagaaactt
     2761 gttcaggatt gacagtcttt gccttgccag gtatgaagaa ataaacggcc ccgcaataga
     2821 cgcgaaagcc cccctgctcg aggaacacct gccacgcatt gtggagctta ccgggaaaac
     2881 aggagctgtc attgtagaac cccggacttt caccataacc tataccctga ctgttccggc
     2941 caacacggtt cccgagggag aaatcctctg cgtgtggatg ccccttccaa gaacggatgt
     3001 cccccggcaa aacggattca ggctgctttc agtctcggaa ccggattata tcatatcccc
     3061 ggattcctac atccataaat ccatctacat ggaaaaaccg gctgtgaagg accaggacgc
     3121 agtgttcacc tattcattca catacacagc ccgggcacag taccaccata ttgatgccat
     3181 aaaggatgtg ctaccctacg atactctcag tcccgaatgg aaggaataca caaaacaaga
     3241 gctcccgcac atcatcacta ccggtcctgt cgcggaactg gcagaaaaag tggtaggtga
     3301 tgaaacaaac ccattcctgc ggttacagaa aattttcacc tacataagcc agacgtatcc
     3361 ctgggccgga gccagggaat actccactat cgaaagcatc ccgctctatg tccttgaaaa
     3421 cggtcacggc gattgcggcc aggtcagcct gctcttcatt tccatgtgcc gaagcctggg
     3481 aattcccgcc cgatgggaaa gcggctggat gctgcacccc ggtgaaataa accttcatga
     3541 ctgggccgag gcctacctgg aaggcatcgg gtggatcccg gttgaccagt cattcggact
     3601 gcagcaggga cctttcccgc tgttctattc cagcggcatg gattcctacc gtatggcggt
     3661 gaacaggggc acgggaggag acttcttccc ggcaaaaatc tatcctcgct ccgaaacagt
     3721 tgatttccaa agaggagaag tcgagtggcg tggaggaaat ctgtatttca accaatggga
     3781 ttaccaactc attttacatc ctttcgacca acagggtatt caataataga ccagtgtgct
     3841 gcgccgggat gtgtcccaga tttccccggc tgattccagg atctgggagg gagtcaccga
     3901 gtctatcagc gacatcattt cccggtgtga gataaccttc ccgaaagaca acaggctttt
     3961 acccatggag aggcattgta cttcatggtt gtcttctgtg accgacatct gtcctgccag
     4021 ttgtttcttg gccctgtcaa gctgcaacgt tgtcagggaa tcctttgcat acctgctgat
     4081 gactttttct gtaagttccc tgcaacgatc caggttgggc ctgtcggtcc caaaatagat
     4141 ccctgcaaat cccgtatcca tgtagggtgc ataggaagct tccactgtat aaaccagccc
     4201 gtatttttct ctgagcagca tattgaggcg tgagttcgag gccggtcccc cgatcatgtt
     4261 catcagaaga accaacggga cacgtttttt atgatgaagg ccgtaagcgg cagcccctgt
     4321 aaggcaatgc gtctggtaag tccccctttt ttccgccata tcaaaccggt tgctgttttc
     4381 taaagggtag tggttggcgg gaaggccggc agcagctgtg cttttctgag aaaacgcacc
     4441 agtgccgaaa taattctctg ccagcaggag cgcctttttc ggggcaacgt ttcctacaat
     4501 gctcacagcc attgttccgg ggaggaaacg ctcccctaca tattgtttca tgtggcggga
     4561 agtgattttt ttcaattctg ccggtatgcc caggatggga gtggccatgg aggtgcccga
     4621 aaacaatttt tcttcaaaac tgtcatagat caggtccatg ggggaatcct tgtaggatct
     4681 gatctcatcc agtatcacat cacgttcctt ggtcacctct gattccggaa acacagaatg
     4741 aaacaccagg tctgacatca gctccagtgc tttgcccagg tcttcgcgca gaactgtagc
     4801 atgcagcacc agttcttcct tggtcgtaaa agcatttatc tctccgccaa gcctttccag
     4861 acggttgttt atgtggtatg attttctgcg cagtgttccc ttgaacagca tgtgttcgca
     4921 aaaatgggca agaccggcaa agcctgatgg ttcaaaacgt gtgccggcaa gggtgctcaa
     4981 tgcacagtaa ctcacaggcg atgtggtggt aagaacggcg cattgcatgc cggaagttaa
     5041 ggtagtcacc atgacggggt ccatagtctt ttcagcaata taacaaggcc gcaaaaatag
     5101 ggattatttt ttatctttgt gccctatgga tccttttgta gtatcagcca ggaagtaccg
     5161 gccacaacaa ttcaacgatg ttgttgatca ggataatatt gttacaaccc tgaaaaatgc
     5221 aatccagagg aaacaactgg ctcatgccta tcttttctgt ggtccccgcg gggtgggaaa
     5281 aaccacctgt gccaggattt ttgccagaac cataaattgc ctggaacctg gccccggcat
     5341 ggaagcctgt ggaacctgtg aatcctgcgt ttcttttgcc gagggaaggt cttactccat
     5401 tcacgagctg gatgccgctt ccaacaattc ggtagatgat atcagggaac tgattgataa
     5461 agtacgtatc ccgccacagg tgggtaaata cagcgtctac atcattgacg aggtgcatat
     5521 gttgtcccag tctgctttca atgcatttct gaaaacactg gaagaaccgc cctcttatgc
     5581 cctgttcatt cttgcaacaa cagaaaaaca caaagtactg cccaccatat tgtcacgttg
     5641 ccaggtcttt gatttcaacc gtatcagcat caaggccatg acacgtcacc tggcagaggt
     5701 cgcccttaag gaaggcgtca caataggaca ggattccctt caggttatag cccaaaaagc
     5761 agatggtgcc atgcgtgacg cactcaccct gcttgaccag tgcattgccg tcagtggaaa
     5821 agaggtgact taccagcagg tgatctcgaa cctgaacgtg ctggacaggg agtattactt
     5881 ccggctaacc agtatggcct tggaaggccg ttttgatacc atgctgctgc tttttgacga
     5941 agtgctggcc cgcggattca acgctttgca tttcataggc gggttgagtt ctcattttcg
     6001 caacctgctg gtatgcaaac agcctgaaag cctgccactg ctcgaatacg gggaggaaac
     6061 cgcccgtaaa tacaacgaac aggcagcagc gtgttcactc ccctttctgt tcggtgccct
     6121 gggtatctgc actcaggccg aggccggata taaacagagc aaccagccca ggttacatat
     6181 agaaatggct ttggtccggc tcagcagggt ccacaaccag gaaacgccaa aaccgcgact
     6241 gaatgatatg acaaccgata ccgggccact ggataatgct ccaggacctg ctgctatccc
     6301 gttccgggca tcaacacccg gccccaccgc tatccagcct cctgcgccgg ttcccgtacc
     6361 ggcgcccaca ccggaacctg ccattacgac atccatcaac agactgaagc atcagaataa
     6421 gaaagaaaag caacgggaag aggaaccctc cggggaaact accggatccg gtgagctgac
     6481 catggaagca ctggaaaagg ctctgcccgg gctgatgaat ctcttcaaag acaaaccgaa
     6541 cctgtccgcc accctttctc gttgcccgcc acttatcgta gacaaatgca aggtgcagtt
     6601 caccgtggtc aatgatctgc agaaaacatg gatagaagag cattatggca ggattattga
     6661 tacatacctg cgcaacaccc tgcggaataa agaagtatgc atgttcctgg tcgtggagga
     6721 agcgcctggc aatatagccg tcaatacgtg ctatatgccg gaagaaaaag caaaatatat
     6781 cgcagaacag catccagaag tggcagccct cagggatgat ttgaacctgg acatcagtta
     6841 accttaggat cttatgcgct tacactgtca taacctggtc aaaagatacg gtttgagaac
     6901 cgtggtcaaa aatgtttcaa tccatgcaga ccagggtgag atcgtaggtc ttctggggcc
     6961 caacggggcc ggaaagacca ccagcttcta catgatcaca gggctgataa aacccaactc
     7021 aggaaagatc ttcctggata atgaggagat tacgacgtac cccatgtacc agcgggccaa
     7081 gaaaggcatc ggataccttg cccaggaagc ttctgtcttc agaaaactga gcgtcgagga
     7141 caacataatg tctgtcatgg aattgtcgac attcacaaaa gcagagcgca aagaaaggat
     7201 ggagcggctg attgctgaat tcggcctgca aacagtccgt aaaagctatg gtatccagct
     7261 ctcgggagga gaacgccgtc gttgtgaaat tgccagggcc ctggcgctag accctaaatt
     7321 cattttgctg gacgagccat ttgccggtgt ggaccccatt gccgtggaag atatccaaca
     7381 gatagtggcc cagctgaaaa ccaaggatat cggtgtattc atcaccgacc acaatgtcca
     7441 cgaaacgctg gccatcacag acagggccta cctgctttat gaaggcagca tcctggaaag
     7501 cgggacagca gaagagctgg cgtcaaattc tgaagtcaga cataaatacc tggggcaaaa
     7561 ctttgaatta cggaaaattg ttccggtagg gaccaacgac atatccgaaa cagataataa
     7621 agaatatgat cccggggagt atcttcctga cggggattat tcagaagacg ccgtgtccag
     7681 ggaaaaagga cttaattaaa ccggatcccg gtaaatccga taatttggag tgccactttc
     7741 ttatagaaat ccggttcaag ccgtctgctg taaggtgcga tatgaaccag gaagttttcc
     7801 atgtcagatt tgtgaatagg atcggccggc ggggattcca tcgtcagggg attgatgggt
     7861 ttcccgtttc tccagacgcg gaaatcaaga tgaggtcccg tagatacccc ggtactacct
     7921 acagatccaa taacttctcc ctgcgccacg cgttttccca caaccagtcc gcgggcaaaa
     7981 cgtgacaaat gaaggtatgc ggtggtatat acactgttgt gcctgatctt tacggtattg
     8041 cctccgcctc gtgtataatt tctttcaatg accgtacctt ctcctatgct caccacaggc
     8101 gttccgaccg ctgctgcgta atcaattcct gtgtgcggcc ttaacgtgcg tgttaccgga
     8161 tgcctgcggg aatagctgaa tccggagctg acccgggaat atttcagggg agccttaaga
     8221 aaagcccgct gcaggttaac cccgtcctga tcccagtatc ccggaagact atcctgtata
     8281 aagcggaaag ccatctggat acggcctgaa cgaatgaaag atgctgccag tatggtaccg
     8341 acttccactt cttttccctc aagcatgtaa gcctggtagg atgccctgaa agtatcccct
     8401 ttctgcagcc cgaaaaaatc caccgtccag gcgaatactt ctgacagccc attggcgacc
     8461 agtggattgt aaccggcatc ctccagatcc tgccataatg aggtagatat ggtgatatcc
     8521 acgaacttat cctcgatttc cagcggaatc tccgcacgcg ttacccagag actgtctgtc
     8581 agagaaaagc atatgtgtgt cctgaaattt tcttcatata tcatataagc gggttgtgag
     8641 acaccggggg catacagtag cctgtaggga tttcccgcct tgagtttgcg caggtcaaaa
     8701 acaccgcggg ctttctccgt gagcagaaac acccgctgga aatccactcc cctgcgttgc
     8761 aggattccgg ccaggttttc attctttctt atcacgtcac tttccttgct gtaatccatc
     8821 aatggcaatg aaaacaattc gcaatacccg gcaatttctc cttccaatat gatcttatca
     8881 gacgactcct gcagttccac gttctcttcg ccagatcctg cacagcaggt aaacacaagg
     8941 cagcacaaca gaaatatgat ttttttcaca tacatgtcaa agggttcttc tgatacaaaa
     9001 ctaaataaaa ttgtcgcaaa gtggaaaatt ttgtaataaa atggaaaata gtggaaaaat
     9061 ttttatactt ttgctccaga cattataccg tatcggcatg gcaaaattta ttggggaata
     9121 caatgcaaag cttgacgaca aaggacgcct tgtgtttccc tctgccctga aagcactcac
     9181 agatcctggc attccgcttc gatttgttgt aaagaaagat ttgttcagtc cctgcctgga
     9241 aatgtattcc tatgaagaat gggaaaaaca gtcagaggca gtaaaggcgc gactgaatcc
     9301 ttcctttaac cgcgagcatg ccgtcctttg gcgtgcttac atgagcaaca gggcagtagt
     9361 cgaacccgac gagaaaacag gaaggatatt aattcccaga catttgctca acatgatagg
     9421 tgtgaataaa gaggtgattt ttgcgggagc cgatttcaag atcgagttgt ggccgaaaga
     9481 ggcctaccag gtcagtctaa tcagtaatga tgattatgta tcacttgcag aaaagatcct
     9541 gtcataacaa tagctcctat ggttttctat catataccgg tccttttgag agaaagtatc
     9601 gaatccctgg ccataaggcc gcagggggca tacgtagatg ccaccttcgg aggagggggg
     9661 cattccaggg caatcctgga acagctggga ccggagggac gcctgctggc atttgaccag
     9721 gactcagatt ctcggaacaa catgccggac gacaaaagga tttcatggat ccattccaat
     9781 ttcaggttta tccacagtgt ttgcaaatac catggcatgc atcaggcaga tggcattctg
     9841 gcagacctgg gcgtttcctg gcatcagttt gatacggaag aacgcggatt ctccttccgg
     9901 tttgatgcgt tgctggatat gaggatgaac cggtcttcag aaaagactgc cgccaatgtg
     9961 gtcaatgaat atgcaaggga agacctggcc tccctttttt ctcgttacgg ggaaatacaa
    10021 aatgccggag ctctggcagg agccattgtg aaagagagaa gcaaacatcc tctcaatact
    10081 tctggagatc tttgtaaagc attgaagagg ttcatgcccc ggaatgcgga gcataaatac
    10141 ctggccaagg tataccaggc gctgcgcatg gaggtcaatg atgaaatgcg ggccctggaa
    10201 ggcttgcttc atcagggcct tgctctcctc aggccgggag gaaggttggt catcatcacc
    10261 tatcattccc ttgaagacag gatggtaaaa caattttttc gtgataagac cgaagccggt
    10321 ttgataaggc tggtaaataa aaagcccatt gtgccgcagg aaagggagat cagtgaaaac
    10381 accagggcca ggagtgccaa actcagggct gctgaaaaat gcggggagga ggaacatggc
    10441 taaaagcaag ggagaaaaga tcgtatccac actgttcggg gggcgtgccc tgctaagcgg
    10501 ggtcctttcc agacacctga acttcatcct gtatatcttt tttctattga tactatacat
    10561 aagcataaac aatgctgtgg aaaaaaccct gttagccaat taccaggtag aaaaagcaat
    10621 aaagctgctt catgcagact acaccagaaa atcggccgat tttatgaagc tgagccaaca
    10681 acaggaaatt gaaagagaac tcaggaacag gggatcggaa ttaatggcgc ccgttaaccc
    10741 gcctgcctgg ataaaaagtg aacgatgaat acgtcagcca aaatgatgat cgtttatata
    10801 ggttatcttc tggtggccat gctggccatc ggacagactg tgcacctgca gttcttttcc
    10861 gattacaagg atcagctgga tgccatagta ttcagaaata tcgaagtgca ggcataccgg
    10921 ggcagcatcc tggattgtga cggacgcgtg ctggcaacta cggtgccttt ttatgaagtt
    10981 cgcatggatt gctccatccc ggatgacttg atgctgaacc gccatctggg tgacctgtcg
    11041 gtagccctgt cacgtttttt tggcaataaa tctgcagcag cttatgaaaa tgagatcata
    11101 caggcacgag tgggaaaaaa gcccgggtac cggaacctgc ttcttggcaa ccggttggtg
    11161 tcttatgatg agctgcagaa aataaagcaa ttccctattt tcagccaggg gcaattcagg
    11221 ggcgggttga ttgccaatca aaaggaaaaa cgaatttatc cctacgggcg tctggcttac
    11281 agaaccatag gctatatcag cgaggatgaa cagaccggcg tgggcattga atacgcctac
    11341 aacagctatc tgaagggaaa cccgggaaaa gaacgtgtac gaaggcgttc aggcgatgat
    11401 aaatgggtgc ccgtttccag tcagccggaa gtggcaccaa aggacgggct ggacatagtc
    11461 accaccatag acatagatat ccagggagct gccgaaagtg ccctcaggga acagctttcc
    11521 gtagactcaa cttttgaagg tgcgaccacc gtggtgatgg aagtggggac gggagccatc
    11581 cgtgcgatcg taaacatgaa aagaatgtcc gacgggactt atgacgaaac attcaactat
    11641 gctatcagtg aatccaccaa tcctggctcg acattcaagc ttgccacact tatcgcgctt
    11701 ctggaagatg gctatgtaag gcttacggac agcattgcca ccggaagaaa cggatggaat
    11761 tattatggaa aaacatttac cgaggctacc agctcttatg gtaccataac agttcagcag
    11821 gcttttgaga aatcttccaa tgtggctttt gccaaactgg cagtggaaaa gtacgggaac
    11881 cgggaaaaac agtttatcaa caggctgtac aacatgaagc ttaatgaaag actcaacatt
    11941 gagcttatag gggaaggacg ggcacagata ccctttccgg gagaaggcgg ctggtcacgc
    12001 ctttcgctgc ccatgatcgc catggggtat gaaatgatgc tcaccccgct tcacgtgctt
    12061 accctttaca atgccattgc caacaacggc aggatggtca ccccctactt catagaaagg
    12121 tttatgcaac acggaaggct ggttgaacag tcggaacgta cccttttgag cggaacgctt
    12181 tgcacctcgg caacgctcaa agccgttcac caggcattgc ggggcgttgt ggagaacggt
    12241 acagccagcc gcattaacga tccgcgttac aatatttcag gaaagacagg aacggcacag
    12301 atagcctatg acggggctta tgtccataac ggttacagga aacaccaggc ttcctttgta
    12361 gggtttttcc cctccgaaga cccaaaatac agcatgatcg tcattttgta ctccagcccc
    12421 acccggggaa atttttacgg cgcttcgtgg gccgctcccg tgttcaagaa aatcgcagac
    12481 catatataca taacaaatcc tttctggaat gccccgctgg gagttacgga aaaaacctga
    12541 atgcatgatg tcatggaatt ggtcaagctt atagaagtat tggtaacagg caggatcacg
    12601 ggatcaaccg cccgtgagat cacctgtatc agccaggatt cacgcaaggt cagcgaagga
    12661 tgtctttttg ttgccgtaca gggagcacaa accgacggtc acctgttcat ttcacaagcg
    12721 tcacgaaatg gtgcagcagc cgtagtatac cagaaaggtt gcctggcaag cgaacaggag
    12781 atggaggcgt tatccgggaa caccacattc atagaagttg gggactcaca gcttgccctg
    12841 gccatgctgg ccgatgcctg gtacggtcac ccctcggcca acatgatcct gacaggagtt
    12901 acagggacca acggaaaaac aactaccgcc accctgctat accggatgtt tggccagctg
    12961 ggacacaaat gcggactgat atccaccatt gcctactata tcggccagga agagattccc
    13021 gccaccaata ccacgcccga cgcactcacc ataaacagcc tgctggacca gatgctgcgt
    13081 aggggttgta cccattgctt catggaagtc agctcacatt cggtggtaca gcaccgtatc
    13141 acgggacttc attttgcagg tggcattttc accaacctga cccatgacca cctggactat
    13201 cataaaacct ttgccgctta ccgggatgcc aaaaagcgct tctttgacat gcttcccgcc
    13261 ggggctttcg ccctggtcaa tgaagatgac aggaatggaa agatcatggt ccagaactgc
    13321 cctgcccgta cgtataccta tgcctgccgg aaaatggccg attacaacgc ccgcatagtg
    13381 gagcatacgg tggacgggat gcagctgatg atagacgggg tggaagtatg gagttctttc
    13441 atagggatgc acaacgccta caatcttctg gcagtttacg cctgtgcagc tatcctgaaa
    13501 gagccccggg aggaaacact ccggatcctg agtggcctga ccacagtcag aggcaggatg
    13561 gaatatgtca ggggaggaga aaaccttacg gccgttgtag attattcgca taccccggat
    13621 gcgctgaaaa acgtcctgct taccctccgg gaactggtaa gaccgggaca agagcttgtt
    13681 tgtgtggtag gatgcggagg taacagggat aagacaaaac gccccctgat ggcccgtatc
    13741 gccgatcaat acgccgatct ctgcattttt acttcagaca atccccggtt tgaagatccc
    13801 gaggacatcc tggatcagat gattgcaggt cttacccctg agcagcagga tcgccacctg
    13861 cgcataaccg acaggcgcca ggcgatcagg acggctataa aaatggctcg tcccggagct
    13921 gttgtactgg tagccggaaa aggccatgag acataccagg atatcaaggg ggtaagaaac
    13981 cacttcgacg ataaagaaat tcttacagaa attttaaaga ccggatgata tgctatacca
    14041 tttatttgaa tacctgaaag aaaacgtgga tttcccggga gttggactct tccgctatat
    14101 atctgtccgg tccataatgt cggtcatcat tgccctgttc gtttcaacga tagcaggaaa
    14161 atacatcata gcccggctca agaggaacca gataaccgac acctcggtaa aaacggggga
    14221 aaacggaaaa tccggaactc ccaccatggg aggcattgtg atcatcgtat ccctgctggt
    14281 atcggtgttt cttttatgtg acctgtccaa cctgtatgtg cagttgctgg tcatatccac
    14341 actctggctt tccactatag gctttcttga tgatctgcta aaactaaggc acatggccgg
    14401 taaagaagac tcccgggtag tgcgttttct gtccagactc cggcgtaagg ataaagacgg
    14461 aatgaaagga aaactcaagg tgtatggtca ggtggggctg ggtttgatcg tagggattgc
    14521 cctgtatgtg agcgataagg cagttatcgt ggaaatgaag aatgttccct cggacacaga
    14581 tcagacagag caggtggaaa cgaccagtta tgtccgtcat gtgaaaagca ccaagacaac
    14641 cattccattc ctgaaagaca acctgtttga ctattcctgg ctcacgccct tcaagggagc
    14701 agccgggcag aaggccggct ggatcgtata tatcctggta gccatcttca tagtgacagc
    14761 cgtatccaac gggaccaacc tcacggacgg gagagacgga ctggccaccg gcacggtggc
    14821 cattgtgggg gtcaccttgg gtgtgcttgc ctatttgacc ggtaatattg tctatgcgcg
    14881 gtacctgaat atcatgtata tacctaattc aggagaaatg gtcgtcttta tggcaggtct
    14941 gatcggggcc ctcattggct tcctctggta taacacctat ccggcacagg tattcatggg
    15001 agatgtggga agccttaccc tgggaggcat cgtggccgtg ttcgcgctga tgataagggt
    15061 agagttgcta ctggtaatac tttgcggcat tttcctggca gagagtttct ctgtaatgat
    15121 acaacggttt tggtacaagt atacccgtat ccgtgccaca aaaaaactga tggcacaggg
    15181 aaaataccat ccgggagatg tcgtgaaggg agaaagggtg tgggtaaggg caccgctgca
    15241 cgaccattac atatacgacc ggaagaaagt tgacaccccc atattgctta agacacccag
    15301 ggaacctatt gcggagccga aagtggtcgt gcgtttctgg atcataagca tcctgttagc
    15361 cgtgttaacg attataacat tgaaaatcag ataatcaaaa tcatactatg ggcaatagac
    15421 tggttatact gggaggcgga gaaagcggtg tgggggccgc agtacttgca aaaaaacaag
    15481 gctatgatgt gttcctttcc gattcagggg tcatccagcc cggggcaagg gctttactcg
    15541 atgaacacaa ggtcccgttt gaagaaggaa tgcatacccg ttcgttgatc atgacagcgg
    15601 atcaggttgt aaaaagcccc ggcatcccgg acactgtacc cgttgttgaa gatttgaaca
    15661 aggccggaat acccgtgatt tcagagatcg aatttgcggg ccgctatacc aatgcccgta
    15721 aaatttgtgt cacagggagt aacggcaaga caaccacggc ttccctgatt tattacctgc
    15781 tcaggaatgc agggctgaat gccgggctgg cggggaatgt gggccagagt tttgcatacc
    15841 aggtagccac gcagaattat gatatatatg ttctggaact gagcagcttc cagctcgaag
    15901 gcatgtatga tttcaaggcc gacattgcca ttcttttgaa tatcaccccg gaccacctgg
    15961 acaggtacca tcatgacctg aatgaatacg tcagggcaaa gttccgcatc acccagaaca
    16021 tggatcagga tgattgcttt atcttttgtc gggacgatga gatttccatg gatcatctga
    16081 gccgcattgt tctgaaagct aaaatgctgc caatttccca acaagtcgtg attccacagg
    16141 gcgcttatac ctcggataac aggatgcatg tgacttatgg tgaggaagaa tttgaaatat
    16201 tcctcaacga actggccctc cagggaaagc acaacattta caattccatg gcggctgccc
    16261 tctcggcaaa ggtcatgcat atcagcaatc ccgttatccg cgaaagcctg acctccttca
    16321 aaagcattcc ccacaggatg gaacaggtgg caagggtaaa aggcgcgctg tatatcaacg
    16381 actccaaggc aaccaatgtc aactcagtat ggtatgccat tgaaagcctg aaaacccctg
    16441 tcgtctggat tgccggaggg aaggacaagg gaaacgacta tacgccatta tacgacctgg
    16501 tctcggaaaa ggttaaggcc ctgatctgcc tgggtgctga caataaaaaa ctccacgact
    16561 gctttgaagg cagggtaccc tgcattattg aaacaaactc catgaaagat gccgtaaacc
    16621 aggcatatgc cctggcgcaa tccggagaca ctgtgctgct ctcccctgct tgtgccagtt
    16681 ttgatctgtt caagaattat gaagaccggg gagacaagtt ccgggaagcg gttaggggct
    16741 tataaaaaag gttatgaaca aaacaggaaa aataaggctg aaaggggaca cgtctttatg
    16801 ggcgctggtg atcatattct cattgctgtc ccttgtcttc gtgtactcat cttccagccg
    16861 tatcgcggcc atgcagaaca catcgacctt ttccatcatg atcgaacaga tggggcacat
    16921 cctcattggt ttcgggatca tctacctgtt ccacatcatt cccttgaaga cttaccggat
    16981 actggcttat ttcggatata tcgtgttcct ggtccttttg ctgctgacca tgttccttgg
    17041 agtcacacgg aatgaaggca caagatggct gtccatattc ggattcagct tccagacctc
    17101 cgagtttgcc aagatcgccc tgatactatt tgtggcaaaa gtgctggagg atgacaaact
    17161 ggacagtttc aaagattttt tcctgcgatt gttccttccg ctgctggcct ttatcctgcc
    17221 gataatctgg gaaggctttt caaccggggt tattctttca ctgaccgttc tgatcatgtt
    17281 gcttatttct caggtccgtt tcagtcatat tgcgaaaacg gccggtatcg tggtattgct
    17341 ggcaggatta acctacgggg gcatgattct gaacaggaca ctacgcaaaa agacggaccc
    17401 gtccctggta tccacctccc gcctggagac ggtggaaaaa cgtgtgagca gtttccttgg
    17461 attcggccag gcagaagagg aaaaggaaaa cgaccaggtc atgtatgcca aggtagccat
    17521 agccacagga ggcgtaggcg ggaagatccc gggcaacggg gctttacgtc acgtcctgcc
    17581 cttgtccaac agcgattaca tatattcaat catagtggag gaaacagggc tgataggcgg
    17641 catatttgtc attggcctgt acttctggct cttgttcagt gtgatcatca tcacgaaaaa
    17701 atgcacgaag atcttttctt ccatgctcgt aagcggattg ggagtattga tcgtaacgca
    17761 ggcaatgatc catatcctgg taaacgtggg attgttgccg gtaaccggcc agactctgcc
    17821 catgattagt tcgggaggtt cttccgtcct ggctgtgagc gctgcgttcg ggatgatgtt
    17881 gtctgtaagc agggcgttgc aggatgagca gcttgactct gaagacccgg caaaagaaga
    17941 aacaaataaa gctataacac atgaaaaccc gcaatcataa caggatcatc ataagcggag
    18001 gaggaacggg cggtcatatc tttcctgcca tttccatagc caatgccatc aggatgaaag
    18061 atcccgaggc cgaaatcctg ttcgtgggtg ccctgggaag gatggaaatg gaaaaagtcc
    18121 ccgctgcagg atacaagatc acaggcctgc ccatcgcggg tctcaggagg gataagtcgg
    18181 tgagcggtgt catggccaat ctcatgctgc ctgtgcgttt gctcaagagt gtgttggctg
    18241 cgggaaggat cgttaaggat ttcaacccca cggtggctgt cggggtgggc ggttatgcga
    18301 gcggccccct tctctggatt gccaggaaaa agaaaatccc ctatatcctc caggaacaga
    18361 atagttttgc aggaaaaaca aataaaatgc tggccagggg ggcagcctat atctgtgtgg
    18421 cttattcggg aatggaacag ttttttcctg ctgacaggat tgtgttgaca ggaaatccca
    18481 tcagagaagg aatccatcct gcaacagcac aggaaaaaaa ggaagccttg ctgcattttg
    18541 gcctggaccc tggtaaaaag acactccttg tcacaggcgg aagccttggt gcaggcaccc
    18601 tgaacagatg tgttagggaa tacctgccgc gcacgaggga gcatcctgtg cagattctct
    18661 ggcaatgcgg gaagcatgga atgcgcctcg cggctgagtc cctgaaggaa taccccggtg
    18721 ttcctgttca tgcgcacgaa ttcattgcca ggatggacct ggcgtacgct gcggcagacc
    18781 ttgtggtctc gcgggcaggg gcgggcag
//
LOCUS       DAOSOF010000023        18103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3109_length_18103_cov_6.004322, whole genome shotgun sequence.
ACCESSION   DAOSOF010000023 DAOSOF010000000
VERSION     DAOSOF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_18103_cov_6.004322"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1401)
                     /gene="secY"
                     /locus_tag="PLM68_02415"
     CDS             complement(82..1401)
                     /gene="secY"
                     /locus_tag="PLM68_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT54253.1"
                     /translation="MKKLIETIKNIFKIEELRKRIVYTVLLLLIYRLGSFVVLPGIDP
                     MQLSNLQAQASGGLVGLLNMFSGGAFGNASIFALGVMPYISASIVIQLLGIMIPYFQR
                     MQREGESGQRKMNQWTRYLTIVILALQGPVYLTNLHTQLPASAFLIEGFSFNIFATVV
                     LIGGTMFIMWLGERITDRGLGNGISLIIMVGIVARLPFAFVAEAGDRLSQSSGGMLML
                     VVELVVLFFIFVASIALVQGVRKIPVQYAKRIVGNKQYGGVRQYIPLKINAAGVMPII
                     FAQALMLFPMIFASFDATRGFAAVMSNMQGFWYNFIFFILVVAFTYFYTAITVNPTNM
                     ADDIKKNGGFIPGIKPGKKTAEYIDSVMSRITLPGSIFLGLVAIMPVFARMLGVNAQF
                     AQFYGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKTGRLKGRSNL"
     gene            complement(1421..1870)
                     /gene="rplO"
                     /locus_tag="PLM68_02420"
     CDS             complement(1421..1870)
                     /gene="rplO"
                     /locus_tag="PLM68_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT54254.1"
                     /translation="MNLSNLKPAKGSVSKRKRIGRGEGSGRGGTSTRGHKGAQSRSGY
                     SRKPGFEGGQMPLQRRLPKFGFKNVNRVEYKAINLSTLQILHEKTGIVDIDVNVLCNA
                     GLVSKDDLVKILGNGELKVKLNVTAHGFSQKAKEAIEAKEGKIAVIE"
     gene            complement(1884..2066)
                     /gene="rpmD"
                     /locus_tag="PLM68_02425"
     CDS             complement(1884..2066)
                     /gene="rpmD"
                     /locus_tag="PLM68_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT54255.1"
                     /translation="MEVWKITQKKSSNSATKRQLDTLAALGIRRINHSVEKAATPVIR
                     GMVEKVRHLVSVEEVK"
     gene            complement(2079..2597)
                     /gene="rpsE"
                     /locus_tag="PLM68_02430"
     CDS             complement(2079..2597)
                     /gene="rpsE"
                     /locus_tag="PLM68_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT54256.1"
                     /translation="MANSRAKKVKATDLELKDRLVAIQRVTKVTKGGRNFSFAAIVVV
                     GDEKGVVGYGLGKAGEVTSAIAKGIEDAKKNLYRIPLNGETIPHEMEAKFGGARVFMK
                     PAAPGSGVIAGGAMRAVFESVGVHDVLAKSKGSSNPHNLVKATVHALLEMRDAYQVAG
                     LRGVKMDKVFNG"
     gene            complement(2605..2967)
                     /gene="rplR"
                     /locus_tag="PLM68_02435"
     CDS             complement(2605..2967)
                     /gene="rplR"
                     /locus_tag="PLM68_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT54257.1"
                     /translation="MALTKQARKQRIKYRIRKRVSGTSERPRMAVYRSNKQIYVQLID
                     DLEGITLTQASSKDASLKEEIKGKTPVQVAALVGKLAAERAREKGITTISFDRGGYLY
                     HGRVKSLADAAREGGLIF"
     gene            complement(2982..3542)
                     /gene="rplF"
                     /locus_tag="PLM68_02440"
     CDS             complement(2982..3542)
                     /gene="rplF"
                     /locus_tag="PLM68_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT54258.1"
                     /translation="MSRIGKLPVNLPKGVSVSVGAGNLVVVKGPLGELKQKVDPDIKV
                     SIEGTELKVERPTDQPRHRSMHGLYRALIHNMVVGVSTGFSIKQELVGVGYRVDVKGQ
                     MLEFNLGYSHDIHFMLPKEVKATVEIARKGANPVLTLTSHDKQLLGTVAAKIRSLRVP
                     EPYKGKGIKFEGEQIRRKTGKSAAAK"
     gene            complement(3555..3950)
                     /gene="rpsH"
                     /locus_tag="PLM68_02445"
     CDS             complement(3555..3950)
                     /gene="rpsH"
                     /locus_tag="PLM68_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT54259.1"
                     /translation="MTDPIADYLTRIRNAVNAGHKIVEIPASKVKTELTRILHEKGYI
                     LSYKIEEGKPSNTIKIALKYHPETKRPAIKKLIRVSSPGLRRYTNVDKMPRVLNGLGI
                     AIISTSKGIITDKEAKTLNVGGEIMCYVY"
     gene            complement(4039..4308)
                     /gene="rpsN"
                     /locus_tag="PLM68_02450"
     CDS             complement(4039..4308)
                     /gene="rpsN"
                     /locus_tag="PLM68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOT54260.1"
                     /translation="MAKESMKAREVKRARMVARYAEKRKALKEAGDWGGLAKLPRNSS
                     PVRLHNRCSITGRPKGYMREFGISRIQFREMASNGLIPGVRKASW"
     gene            complement(4312..4914)
                     /gene="rplE"
                     /locus_tag="PLM68_02455"
     CDS             complement(4312..4914)
                     /gene="rplE"
                     /locus_tag="PLM68_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT54261.1"
                     /translation="MAKAVKKQQQAPVVVKGYVPALQKKYREEIVPRLMKEFGFTSVM
                     QVPRLEKIVLNEGVGSATADKKLIEVAMSELTLIAGQKAVQTFSKKDISNFKLRRGMP
                     IGVKVTLRSAKMYEFLDRLISIALPRIRDFKGIAEKFDGRGNYTLGIKEQIIFPEIDI
                     DKISKILGIEITFVTSTGKDEEAFALLREFGLPFKNIKKG"
     gene            complement(4914..5237)
                     /gene="rplX"
                     /locus_tag="PLM68_02460"
     CDS             complement(4914..5237)
                     /gene="rplX"
                     /locus_tag="PLM68_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT54262.1"
                     /translation="MSKKLHIKKGDTVFVNTGEDKGKTGRVLEVRPQEQRAIVEGINM
                     ISKHTKPSAKSPQGGIIKKEAAIHISNLQVVDPVKGGPTRIGRRLNSKGKLVRYAKKS
                     GEEIK"
     gene            complement(5248..5616)
                     /gene="rplN"
                     /locus_tag="PLM68_02465"
     CDS             complement(5248..5616)
                     /gene="rplN"
                     /locus_tag="PLM68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT54263.1"
                     /translation="MIQQESRLLVADNSGAKEVLCIRVLGGTKRRYARIGDTIVVSVK
                     SAIPGGGEAKKGSVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNNAGELRGTRIFGP
                     VARELRDSYMKIVSLAPEVL"
     gene            complement(5619..5873)
                     /gene="rpsQ"
                     /locus_tag="PLM68_02470"
     CDS             complement(5619..5873)
                     /gene="rpsQ"
                     /locus_tag="PLM68_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT54264.1"
                     /translation="MERNLRKERTGLVVSNKMDKSIIVAVNRKVKHPIYGKFVNKTTK
                     FVAHDPNNECSEGDTVRIMETRPLSKTKCWRLVEIVEKVK"
     gene            complement(5884..6084)
                     /gene="rpmC"
                     /locus_tag="PLM68_02475"
     CDS             complement(5884..6084)
                     /gene="rpmC"
                     /locus_tag="PLM68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT54265.1"
                     /translation="MKAPEIRELSVKDLSARIEAEKANLLRLKMNHTISPVDDLSQIK
                     KARRNIARMLTILSETQTKQNL"
     gene            complement(6095..6520)
                     /gene="rplP"
                     /locus_tag="PLM68_02480"
     CDS             complement(6095..6520)
                     /gene="rplP"
                     /locus_tag="PLM68_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT54266.1"
                     /translation="MLQPKKTKFRRQQKGRMKGIAQRGNQLAFGSFGIKTMESCWLTG
                     NQIEAARQAVTRYMKREGQIWIRIFPDKPFTKKPAEVRMGKGKGAPEGFVCPVTPGRL
                     LIEVEGVPFEVAKEALRLGAQKLPVNTKFVVRKDYVEQN"
     gene            complement(6710..7768)
                     /locus_tag="PLM68_02485"
     CDS             complement(6710..7768)
                     /locus_tag="PLM68_02485"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT54267.1"
                     /translation="MKVLFLLCLLTQVPVTDSLDLLVERVHDSMISVDTHNDFSMQLA
                     FPEQRATVTKGQVSFELMKTGRLDAAFFAAYQGQGPCTGEDHGRAKYLADSMLMALHR
                     YAREHRQLGAVAYSAADVRTLKKQGKASLLLSIENAYCLGRDISLVEHYYRMGVRMIC
                     LTHNGNNCVADAAMDSLETHGGLSPFGYQVIQEMNRLGMIVDVSHASRKTCLQAVQAS
                     KTPVMASHSGVYNLKKIPRNLTDEEIIAIASRGGLIQISIGRYFLSNLPKAEVGMEHL
                     LEHINYVVDLVGIDHVGIGSDFDGGGGIVGVEDMGKMKAITRALLEQGYTEGEIGKLW
                     GGNVLRVMEEVARNADGF"
     gene            7835..8791
                     /locus_tag="PLM68_02490"
     CDS             7835..8791
                     /locus_tag="PLM68_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT54268.1"
                     /translation="MKRFLLSFLLLFTFTLPAVAQKNTYRLLLHWYPQAQFAGYYYAL
                     EKGFYKDADLDIQIIYGTVTTPSSQILKSGDFDFATLWLSTAMQLKASKVPLQLLAQT
                     NQSSALMLVSHKGRGVDRLEDFAGKKIGIWSGDFELQPLSLLSRNNINMQVIIINNSI
                     NLFLRGAIDVCMAMWYNEYHEILSAGLREEDLNIFRMSDYGMNFPEDGIYVHEKLAWN
                     RPGVCKKFVEASLRGWMAAFEDVEGTLDILEKACNNAGVPFSRAHQRWMLNCFRELLI
                     HPRTNKIEPTLPVHAYNFVGSVLLERNFIKSIPPYETFVANL"
     gene            8766..10838
                     /locus_tag="PLM68_02495"
     CDS             8766..10838
                     /locus_tag="PLM68_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOT54269.1"
                     /translation="MKHSSQTFKKRKSISYTIARYVFAGTVLILVVILGLNYSSSKKH
                     MTAVILEKAQNQAENFAGRINQVFASAQRIPTVMASQLNQMEDFSSQDLSRMLESMLL
                     ENPDIFGATIAFEPGMYQGKPHWAPYVYREDSVLKSTLLYDGENNYFMDDWYIVPMQL
                     QEPFWSDPYYETSTNNEDVLMITYSVPFFKERDGKRVVAGITTVDISLLWINNLMESL
                     DVYETGYGFLITNSGRYVYHPDQRLRMNETTFSAMDEWLVNNKNLSTAEADTIRNQQF
                     SVYRKMLAGESGLFMDSFMFPDWEEKAWFAYVPIRSNHWSLALVYPRDEALAEVRSLN
                     TILILVGFFSLFVLLGFIFIVTRVLTLPIVNLTRYTKSVAESGDYTRSVPELSSKDEI
                     GELAYSFSILMKEIDQAQKTLEDRVRERTAQLVEAQDLLVQSEKMASLGQLTAGVAHE
                     IKNPLNFINNFSELSVDLTKELKEALASVDMNENDKSYVNEILGDLSMNAAKILEHGK
                     RADSIVKGMLLHSRGKSGEFQLTDINNMLTEDIALGYHGMRAQDNTFNINIEQDLDKD
                     LPLISVVPQDLSRVFLNMINNACYAVKDAGPSKPSGWTPTLRISSRKAGNFLEVRIRD
                     NGKGISPENIKKIFDPFFTTKPAGKGTGLGLSLSYDIVVKVHGGQIQVESEPGEYAEF
                     IVTIPIKQ"
     gene            10845..12026
                     /locus_tag="PLM68_02500"
     CDS             10845..12026
                     /locus_tag="PLM68_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT54270.1"
                     /translation="MAAEKILVVDDETDLQYVINQKFRRQIRNDQYEFHFAFNGLEAL
                     AKLIEVPDIGVILSDINMPEMDGLTLLSKVRELKNPLLKTVICSAYGDMDNIRTAMNR
                     GAFDFVTKPVDFNDLEITLEKTIGELNIVREGLAYHDKLLSIEHDLMVAREIQRAIMP
                     KAIPGLEWADIDGSMEAARQIGGDFYDYFMMDDHRLGFVIGDVSGKGIPAAIFMAVSR
                     TLIRATGLKGMSPRDCMNYVNHLLCTESVNSMFVTVFYGMLDLETDTLHYVNAGHNPP
                     ILLRHNGSTEVFPMTDNIVLGVYDGYSFKTNSIPFLKDDTLVMYTDGVTEAFNQNNEE
                     YGFETLHTLLKEQVLGNKEVMDGKDAPARICLIIADDVNRFAGEEEQSDDITIMVVKR
                     N"
     gene            12036..12449
                     /locus_tag="PLM68_02505"
     CDS             12036..12449
                     /locus_tag="PLM68_02505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54271.1"
                     /translation="MKRTLELENQLSALSGLLDMTGDFLDAHKVPVPVQNKIKLILDE
                     IFSNIVSYGYLPQDPPDTVLVELCIANGKFTAMFNDGGMPFNPLEYGPVDTSLPLEER
                     PIGGLGIHLIRNLATELAYERKENRNVFCFSIEIE"
     gene            12446..13123
                     /locus_tag="PLM68_02510"
     CDS             12446..13123
                     /locus_tag="PLM68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOT54272.1"
                     /translation="MKTKLTIAALVLYLLAPLCLQAQTAKTSKGYLMGYVVENGDTLF
                     IATIPDLHVYSRDKKKSDREWREYYRTVHNFARAYPFALQARERMDRADSILEVSNFS
                     PRERERFLEKTERDLFAEFEAPLRKLTFTQGRILLRLIDRELGQTSYAVVKSYRGGLT
                     ASFWQGVARIFGADMKKPYDKYGEDKLLEELVRMYHDGRFYYLYFSLFGPPKDTNISP
                     YGTRWAN"
     gene            complement(13016..13792)
                     /locus_tag="PLM68_02515"
     CDS             complement(13016..13792)
                     /locus_tag="PLM68_02515"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOT54273.1"
                     /translation="MSTYYFVADIHLGLRNGDPSDREARFIAFLDSLAPGTGGLFLLG
                     DIFDFWYEYKYVIPRGYTRTLGALARVHDRGIPVHFFVGNHDLWTYGYLEQELGMQVH
                     RDEWYVKLEGKYFLLAHGHRMGRQPCASRFLHGMFHNRLLQVLFSCLHPRWAFALARK
                     WSAWNRDKHNGSCKQDMNLYKPVYQYAATYDRPVDYCITGHIHTSERCPLPAGGELIV
                     LEDWLSVGPSGPVRGDIGIFRGTEKGEIEIIKAPVVVHAH"
     gene            complement(13789..14595)
                     /locus_tag="PLM68_02520"
     CDS             complement(13789..14595)
                     /locus_tag="PLM68_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT54274.1"
                     /translation="MKGFEALILGLLQGLTEFLPVSSSGHLVIVKTLFGIETEDASFE
                     IVVHLATVLSTIVVFRKELWEMVSDTLRFRNTPATGLTLRLLFSAIPVLIVGLFFKEK
                     VETLFSGGLTIVGWMLLLTAVLLMVSQWLSALRHKKGSVRPIGYREAFLIGIAQAFAV
                     LPGLSRSGATIATGLSLGGNKDKVAPFSFLMVLIPVLGEAFLEILGGGFAPARTGITF
                     LALAIGFAAAFVSGLLACKLMITIVRKVRFTWFAVYCMLAGLACLIVPLL"
     gene            complement(14582..14857)
                     /locus_tag="PLM68_02525"
     CDS             complement(14582..14857)
                     /locus_tag="PLM68_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOT54275.1"
                     /translation="MKKEKKNEQETKGKFALPARNVYLLLIGLVVIALGFVLLAGGGS
                     DDPGVFTGQALFSFRRMVIAPLLICGGFVFEIIAIMHVKKDKGNERV"
     gene            complement(14864..15769)
                     /locus_tag="PLM68_02530"
     CDS             complement(14864..15769)
                     /locus_tag="PLM68_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT54276.1"
                     /translation="MEPLTMAQQEKNIINQQLVQSYLSSVVSISLVLLLIGITGLLAV
                     NARTVSDYFKEHLGVSVILDMETKDADSTLIIKQFLATPYVRDAVREAVFISREKGAA
                     EMAQLLGSEFLDIFEYNPLPASVELRLNADYVNNDSIARMETYLLKLLYVEEVSYQAP
                     LITLINRNLRNIGVVLGVIILVLLIISFALIHNTIRLSVFARRFTIYAMRLVGATRRF
                     IRKPFMTQALIQGFLSSLIAIVLLIGILYMLQREFPGILMLVSREMFLIVMGAMILTG
                     ILLCRISTFFVVNRVVKLESSAFYY"
     gene            15829..16656
                     /locus_tag="PLM68_02535"
     CDS             15829..16656
                     /locus_tag="PLM68_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT54277.1"
                     /translation="MIKAKKILYVSSEIFPFLPQTDMSYVGRHLPQAIQESGGEIRSF
                     MPKYGCINERRNQLHEVIRLSGMNIIIKDIDRPLIIKVASISTARMQVYFIDNEDYFQ
                     RKSTYRDANGEFFADNDERGIFFARGVLETVKKLRWKPTLVHCHGWLSHLLPLFLKKA
                     YHDDPLFTNVRVVVSLYNDLTTESFNEKMRSKIIMPGIKAKDMDFLEEPDAVNLAKTA
                     VQYAHGVILAHPGVDSSIAKYAGSLNIPVMPYIDPQDPKSNYIKEYNNFYDQILETQ"
     gene            16653..17990
                     /locus_tag="PLM68_02540"
     CDS             16653..17990
                     /locus_tag="PLM68_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOT54278.1"
                     /translation="MTIRACGIILSASLLFLISSCITVDNRMGADFIPTNQILHINSV
                     ELEAPMFTAVADSMNMSYPAYLAFGSVNFPVFGKTTTSALVQFAPYSYQKEYGADPIV
                     SDMFVNIPLGGFTVSGDNDRYIPQNVYVYKIIKDLDKLDVYHKSFSEEFIDPIPVSKP
                     GQIFLGRDTLRIEFTEEFAYELLQADSIERDSTEAFIERFKGLYITTDDLNTSETGGR
                     MNFLDITEANIYLNYKSGQGDSLLTYFFNIYGTYYNVSSHQSSHLATNSPAAHLYYEG
                     LAGVKPCLDASNMRDRIKTWGLAQTPPVDASQILISRADLVLPVEIPLNNDYSTVDKA
                     PGNLFPSRLVSNDTLTFYSPLNEIYYQNSGGTMNRSRWEYTFEITTYLQDLLNKDTIT
                     SRDNLWIMPTYVVSGTSADTYYIDNATYRTMILNGNLSDRPPYVRITYAVLLQ"
     gene            18023..18095
                     /locus_tag="PLM68_02545"
     tRNA            18023..18095
                     /locus_tag="PLM68_02545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18056..18058,aa:Lys,seq:ctt)
BASE COUNT         4723 a         4578 c         4012 g         4790 t
ORIGIN      
        1 tcccgttttc acgcaaaagc gctatttctt cttcggtttt cgtaatattc attaaacaaa
       61 aaacaatctc taatccgtat cttacaaatt ggagcggcct ttaagccttc ctgtcttcat
      121 aagaccatcg taatgtcgca tcagaagatg actttcaatc tgctgtagcg tgtctaacac
      181 aacacctaca aggatcagca gagaagtacc tccgtaaaac tgtgcaaatt gtgcattaac
      241 tcccagcatc cttgcgaata caggcataat ggccaccaat cccaggaaaa tggaacccgg
      301 aagtgttatg cgggacatca cactgtcaat gtactcggct gttttcttac ccggtttgat
      361 cccgggaatg aatccgccgt tcttcttaat gtcatcagcc atattggtgg gattgacggt
      421 aatagccgta taaaaatacg taaaggcaac caccaatatg aaaaatataa agttatacca
      481 aaaaccctgc atattactca tgactgctgc aaagccccgt gttgcatcaa agctggcaaa
      541 aatcatgggg aaaagcatca gagcctgggc aaaaataatg ggcataactc cggctgcatt
      601 gatcttcagg ggtatatatt gtcttacgcc cccgtattgc ttgttgccta caatgcgctt
      661 tgcatattgt acaggaatct tgcgcactcc ctgtaccaaa gcgatggatg caacaaagat
      721 gaaaaacagg acaaccagtt caaccacaag cataagcatg ccgcccgatg actgggaaag
      781 cctgtcacct gcctcggcca caaaggcaaa aggcaaacgt gccacgatcc caaccatgat
      841 gatcagggaa atcccgttcc ccaaaccgcg gtctgtaatc ctttctccaa gccacataat
      901 gaacatggta cctccaatga gcactaccgt ggcaaagatg ttgaatgaaa aaccttctat
      961 caaaaaagcc gatgccggaa gttgtgtgtg caggttggta agatagacag gaccctgcag
     1021 ggccagtatc acaatggtca ggtaacgtgt ccactggttc atcttccttt ggccgctttc
     1081 tccttccctt tgcatccttt gaaaataagg gatcatgatc ccaagaagct gtataacaat
     1141 ggatgccgag atataaggca ttacccccag ggcaaaaatg gaagcatttc caaaggcgcc
     1201 tccagaaaac atattcagca aacccaccag acctccggaa gcctgtgcct gcagattgct
     1261 cagctgcatg ggatcaattc ccggcaggac aacaaagctg cccagcctgt agatcagaag
     1321 aagcaacact gtatagacaa tccgcttgcg cagttcttca atcttgaaaa tgttcttaat
     1381 ggtttctatc agttttttca ttggtcaggc gtattctgga ttattcaata actgcaatct
     1441 tcccttcctt tgcctcaata gcttctttcg ccttttggga aaaaccgtga gcggtaacgt
     1501 tcaatttaac tttaagttca ccattcccca ggatttttac caggtcatcc ttggatacca
     1561 gacctgcatt acataaaaca ttcacatcga tatcgacgat ccctgttttt tcatgaagta
     1621 tctgtaaagt ggaaaggttt atggccttat actctacacg gttcacgttc ttgaatccga
     1681 atttcggaag acggcgttgt aagggcatct gccccccttc aaatccgggt ttgcgggaat
     1741 atccggaacg tgactgtgct cctttatgtc cgcgtgtaga tgttcctccc ctgcccgatc
     1801 cttcgccgcg acctatgcgt tttctcttgg aaactgaccc tttggcgggt tttaaattag
     1861 ataagttcat tatgtttcct cctttacttt acttcttcaa ctgacaccaa atgacggacc
     1921 ttttctacca tgcccctgat aacaggggtt gctgctttct caacactatg gttaatcctg
     1981 cggatgccta aagcggcaag ggtatccagc tggcgtttgg tagcagaatt actgcttttt
     2041 ttctgtgtta ttttccatac ttccatgatc tctcctcctt atccgttaaa aactttatcc
     2101 attttgacac ccctgagacc ggcgacctgg tatgcatcgc gcatttccag caaagcatga
     2161 acagtggctt tcaccagatt gtggggattg gacgatcctt ttgattttgc caggacatcg
     2221 tgtactccca ccgattcaaa gacagccctc atggcaccac cggctatcac tccggatccg
     2281 ggtgcggcgg gtttcatgaa tacgcgggca ccgccaaact tggcttccat ttcgtgagga
     2341 atagtttctc cgttcagtgg aatgcggtac agattctttt tggcatcttc aatacctttt
     2401 gctatggccg aggttacttc tccggcttta cccagtccgt atccgaccac ccctttttca
     2461 tccccgacaa ccacaatggc tgcaaaactg aaattacggc cgcctttggt aactttagta
     2521 acacgctgaa tggccacaag acggtctttc aattcaagat ccgtggcttt tacttttttt
     2581 gctctactat ttgccatatc cgtattagaa tattagaccg ccttccctgg cggcatctgc
     2641 taaactttta actcgtccgt gatagagata ccctccacgg tcaaaagaga ttgtggttat
     2701 ccccttttcg cgggcacgct ctgctgccag ctttccaaca agggctgcaa cctgaacagg
     2761 tgttttgcct tttatctctt ctttcaggga agcatcttta gatgaagcct gtgtcagggt
     2821 gataccttca aggtcgtcta tcagttgaac atatatttgc ttattactcc tgtatacggc
     2881 catcctggga cgttcagaag ttccggaaac acgcttgcgg atgcggtatt tgatccgttg
     2941 ttttcttgct tgttttgtta aagccatgat ttcacctcct actatttagc tgctgccgat
     3001 ttaccggttt tccggcgaat ttgttcaccc tcgaatttaa taccctttcc tttatatggt
     3061 tcgggcacac gcagtgagcg gatcttggcg gcaacggtgc caagcagctg tttgtcgtgg
     3121 cttgtcaggg tcaggaccgg attggcgcct tttcgggcga tctcaacggt tgcctttact
     3181 tctttgggca gcatgaaatg gatatcatgg gagtaaccca gattgaattc cagcatctgt
     3241 ccttttacat caacgcggta tcccacgcct accagttcct gcttaatgga gaaacccgtt
     3301 gagacaccaa ccaccatgtt gtggatcagg gcgcggtaca aaccgtgcat agacctgtga
     3361 cggggttggt ccgtaggacg ttccacttta agctctgttc cctctatcga caccttgatg
     3421 tccggatcca ctttctgttt cagttctccg agagggcctt taaccacaac caaattgccc
     3481 gcgcctacag atacgctcac gccttttggc aggtttacag ggagttttcc tattcgtgac
     3541 attttttcta ttgattaata tacgtaacac atgatttcac caccgacatt caatgttttg
     3601 gcttccttgt cggtgataat gcctttggat gtggatatga tggcaatgcc cagtccgttc
     3661 agaacgcggg gcattttatc cacattggta taacgcctca aaccgggact tgaaacgcgt
     3721 atgagctttt taatggccgg gcgctttgtc tcgggatggt acttaagggc tattttaatg
     3781 gtgtttgagg gtttcccttc ttcaatttta taactgagaa tatatccctt ctcgtgtaaa
     3841 atacgggtga gttccgtttt tacttttgat gcgggaatct ccacgatctt gtgtcctgcg
     3901 ttcaccgcat tacgaatcct tgtcagataa tctgctattg gatcagtcat gataaaagtt
     3961 tttaaattgt gcgttcggca ataaattgcc cgatatccaa taattatata tgtatggccg
     4021 gcccgcggag ggcctttttt accagcttgc cttacgtacg ccggggatca ggccgttgct
     4081 tgccatttcg cggaactgga tacgggaaat gccaaattcc cgcatatagc ctttaggcct
     4141 gcccgtaatg gaacaacggt tgtgcagacg taccgggctg gagtttctgg gcagttttgc
     4201 caacccaccc caatcaccgg cctctttcag ggctttcctc ttctctgcat atctggctac
     4261 catccgggcc cgcttcacct cgcgcgcttt cattgattct tttgccatct attatccttt
     4321 tttaatgttt ttaaacggta atccaaattc cctgagcaag gcgaatgctt cctcgtcctt
     4381 accggtagat gtaacgaaag tgatctcgat ccccaaaatt ttggaaatct tgtcaatgtc
     4441 tatctccggg aatatgatct gttctttgat ccccagggta taattgcccc ggccgtcaaa
     4501 tttttcagca atccccttga agtcgcgaat acgcggcaaa gctatggaga tcaaacgatc
     4561 caggaattca tacatttttg ccgagcgtag tgttactttt acgccaatgg gcattcccct
     4621 gcggagtttg aagtttgaaa tatccttttt ggaaaatgtc tgaaccgcct tctggcctgc
     4681 aataagcgta agttcgctca ttgctacttc aataagtttc ttgtcagccg ttgccgagcc
     4741 cacgccttcg ttcaaaacga tcttttcgag cctgggaacc tgcataaccg aggtgaaacc
     4801 aaattctttc atgagcctgg gaacaatttc ttccctgtac tttttctgta gtgccggcac
     4861 atagcctttt accaccacag gggcctgttg ctgtttttta actgcttttg ccattatttg
     4921 atctcctctc ccgatttttt tgcataacgt accaatttgc ccttgctgtt gagccggcgt
     4981 ccaatgcgtg tgggaccacc cttgacgggg tccaccacct gcagattgga tatgtgtatg
     5041 gcagcttcct tcttaatgat tcccccctgg gggctttttg ccgatggttt ggtgtgcttg
     5101 ctgatcatgt tgatcccttc aactatggcc cgctgttcct ggggccttac ctccagcacc
     5161 cttcccgtct ttcccttatc ttccccggta ttcacaaata ccgtgtcgcc tttctttatg
     5221 tgcaattttt tactcataac tcaattccta taatacctcc ggggctagtg atacaatttt
     5281 catataactg tctctgagtt ccctggctac cggaccaaat atgcgcgtgc cgcgcaattc
     5341 tccggcattg ttaagtaaaa cacaggcgtt atcatcaaaa cggatataag aaccgtcggg
     5401 acgacggatt tcttttttgg tcctcaccac cacggctttg gagacggagc cttttttggc
     5461 ttcccctccg ccgggtatgg cacttttgac cgagactact atggtatcac caatgcgggc
     5521 ataccggcgt tttgtgccgc ccaggacacg gatacaaagg acttcctttg ctccgctgtt
     5581 gtcagccact agtaaacgtg attcttgctg tatcatggct atttcacttt ttcaacgatt
     5641 tctaccaaac gccaacactt ggttttactc aggggccgcg tttccataat acgtacggtg
     5701 tcaccttcgc tgcattcgtt gtttggatcg tgcgcaacga atttggtggt tttattcaca
     5761 aacttaccgt atatgggatg ttttaccttc cggtttacag caactattat ggacttatcc
     5821 attttattgc tgaccaccaa tcccgttctt tcttttctaa gatttctttc catgaatcaa
     5881 tgattataga ttctgtttgg tttgtgtttc actcaaaata gtaagcatcc gtgcaatatt
     5941 gcggcgtgct ttctttattt gtgataaatc atctacggga gagatggtat ggttcatttt
     6001 aagacgaagc aaattggctt tttccgcctc aatacgagca ctaagatctt tcacagataa
     6061 ctcgcgtatt tcaggtgctt tcattgttgt taaactaatt ttgttctaca taatccttgc
     6121 ggaccacaaa tttggtgttg acaggcaact tctgggcgcc caaacggagg gcttccttag
     6181 caacttcaaa aggaacgcct tccacttcta tcaggagtct gccgggtgta acagggcaga
     6241 caaatccttc cggagcacct ttcccttttc ccatacggac ttcagcaggt ttctttgtaa
     6301 agggcttgtc cggaaatata cgaatccata tctgtccttc acgtttcatg taacgtgtca
     6361 ctgcctgacg ggcggcttca atctggttac cagtcagcca gcaggactcc atagtcttga
     6421 tcccgaacga cccgaaggct agttgatttc ccctctgggc aatccctttc atacggcctt
     6481 tctgttgcct tctgaacttg gtttttttag gttgtaacat tgctttttct aaacgtttaa
     6541 cgtaacgtaa atatgtattg ttgcttcggt gctaatttgg tgggggtgta ctttgtaacc
     6601 ccttgttttg caatttatta ggaacaaact accctgaaat tgcgggttgc aaagatagaa
     6661 cttaatttct gatttacaaa tatttatgaa caaaagaaag tgaaaaatgt tagaaaccat
     6721 cggcattccg ggcaacttcc tccattaccc gcaaaacatt gcctccccac aatttgccaa
     6781 tttcaccttc tgtatatccc tgttcaagca gtgccctggt tatggccttc atctttccca
     6841 tgtcttcaac acccacaatg cctccccctc cgtcaaaatc actacctata cccacatggt
     6901 caattcccac aaggtccaca acataattga tgtgttcaag caagtgctcc atgcccacct
     6961 cggctttagg caggtttgaa agaaaatacc ggcctatgga aatctgtatc aggcctcccc
     7021 tggaggcgat ggcaataatt tcctcgtctg tgaggttcct gggtattttt ttcaggttat
     7081 aaacccctga atggcttgcc ataacaggag ttttgcttgc ctgtactgcc tgcaggcaag
     7141 tcttgcggga ggcgtgggat acatctacta tcatccccag acggttcatt tcctgtatga
     7201 cctggtaacc aaaaggtgaa agccctccgt gggtttccag gctgtccatg gcggcgtcag
     7261 cgacacaatt gttgccgttg tgggtaaggc atatcatcct tacacccatc ctgtagtagt
     7321 gctctaccag ggaaatgtcc ctgcccagac aataggcatt ttctatagat agcaacaggg
     7381 aagccttccc ctgctttttc agtgtcctca catcggctgc actgtaggcc acagcgccca
     7441 gttgcctgtg ctcccgggca tacctgtgca gggccatcag catgctgtca gccaggtatt
     7501 tcgctcttcc atggtcttcc ccggtacaag gtccctgtcc ctggtaggca gcgaaaaagg
     7561 cggcgtccag acggccggtt ttcatcaact caaaggaaac ctgtcccttt gtcacggtag
     7621 cacgttgttc aggaaaagcc agctgcatgg aaaaatcgtt atgtgtatcg acagagatca
     7681 tggaatcatg cacccgctca accagaagat ccagtgaatc agtgacggga acttgtgtca
     7741 acaagcataa aagaaataac accttcattg tacaaatata gagggtgaat tctaattttg
     7801 ttacatttgt gtctataaaa tgctcgccgg ccgtatgaaa cgttttctat tatccttcct
     7861 tttactgttt accttcactc tgccggctgt tgcccaaaaa aacacgtaca ggttactgct
     7921 acactggtat cctcaagctc agtttgcggg atattactat gcccttgaga aaggatttta
     7981 caaggacgca gacctggaca tacagataat ctacggcacc gtcacgaccc cttcctccca
     8041 gatcctcaag tcaggagatt tcgattttgc caccttatgg ctctccacgg ccatgcaact
     8101 caaggcttcc aaggttccct tgcaactcct ggcgcagacc aaccagagtt ctgcgctgat
     8161 gcttgtaagc cacaaggggc ggggcgtgga cagactggaa gatttcgcag gcaagaaaat
     8221 cggcatatgg agcggggatt ttgaattgca gccattgagc cttctcagca ggaacaacat
     8281 aaatatgcag gtcatcatca tcaacaactc catcaacctg ttcctgcggg gtgccattga
     8341 tgtatgtatg gccatgtggt acaacgagta ccatgaaata ctcagtgccg gtctcaggga
     8401 agaagacctg aatatattcc ggatgagcga ttatgggatg aatttccctg aagatggtat
     8461 ttatgtacac gaaaagcttg cctggaaccg gcccggtgtc tgcaaaaagt tcgttgaagc
     8521 ctctctgcgt ggatggatgg ccgcctttga ggacgtggaa ggaacgctgg atattctgga
     8581 aaaagcctgc aacaatgcag gggtaccatt cagccgcgcc catcagcgct ggatgctcaa
     8641 ctgtttccgg gaactcctca tccacccccg caccaataaa atcgagccta cacttcctgt
     8701 acatgcctat aattttgtgg ggtctgtgct tctggaacgc aatttcatca aatccatccc
     8761 tccttatgaa acattcgtcg caaaccttta agaaaaggaa aagcatttcc tatactattg
     8821 caaggtatgt atttgcaggg acggtcctga tcctggtcgt tatcctgggt ttgaattata
     8881 gttcttccaa aaagcacatg acggccgtga tccttgaaaa ggcgcaaaat caggccgaaa
     8941 attttgcggg aaggatcaac caggtttttg cctcggctca aaggattccc acggtgatgg
     9001 cttcacagct taaccagatg gaagactttt cttcccagga ccttagccgg atgctggaat
     9061 ccatgctcct tgagaatccc gacatatttg gagccaccat tgcctttgaa cccggcatgt
     9121 accaaggcaa gccgcactgg gctccttacg tttacaggga agattccgtg ctgaaatcca
     9181 cgcttctgta tgatggcgag aacaactatt tcatggatga ctggtacata gttcccatgc
     9241 agttgcagga accattctgg tccgatccct attacgaaac ttccaccaac aacgaagatg
     9301 tgcttatgat aacatattcc gtcccatttt ttaaagaaag ggacggaaaa agagtggttg
     9361 cgggcattac gactgttgat atttctctcc tctggataaa caacctgatg gaatcccttg
     9421 atgtttacga gaccggttat ggtttcctta tcaccaacag cggccggtac gtataccacc
     9481 ccgaccagcg cctgaggatg aatgaaacca ccttttccgc catggatgaa tggctcgtga
     9541 acaataaaaa cctttccacg gcagaagcag acaccatccg caaccagcaa ttcagtgtct
     9601 accgtaaaat gctcgccggc gaaagcggtc ttttcatgga ttccttcatg tttcccgatt
     9661 gggaagaaaa agcctggttc gcttatgtgc ccatccgttc aaaccactgg tcgctagccc
     9721 tggtttatcc tcgggacgag gcccttgcag aggtgcgaag cctgaacacc atcctgatcc
     9781 tggtgggttt cttttcgctt tttgttttat taggatttat ctttattgta accagggtgc
     9841 tgacattgcc cattgttaac cttacacgct atacaaaatc agttgccgaa agtggagatt
     9901 acacccgctc ggtacctgaa ttgtcttcga aagacgaaat tggcgagctg gcctattcct
     9961 tctccatctt gatgaaggaa attgaccagg ctcaaaaaac attggaagac cgggtgaggg
    10021 aacgtaccgc acagctggta gaggcacagg atttattggt ccagtcggaa aaaatggctt
    10081 ccctgggaca gctgactgcc ggcgttgcgc atgagatcaa gaatccgctc aatttcataa
    10141 ataacttctc ggaattatcg gtagatctta ccaaagaact caaggaagca cttgcgtctg
    10201 tagacatgaa tgaaaacgac aaatcctatg tcaacgagat cctgggagat ttgtccatga
    10261 acgccgccaa gattctggaa cacggcaaac gcgcagacag catagtcaag ggaatgctac
    10321 tccactccag gggcaaaagc ggggaattcc agctcactga tatcaacaac atgctgacag
    10381 aagatattgc tttgggttac cacggcatga gagcacagga caacacgttc aatatcaaca
    10441 ttgagcaaga cctggacaaa gacctgcctc taataagcgt ggttccacag gatttgagca
    10501 gggtattcct gaacatgatc aataatgcct gctatgctgt taaggatgcc ggcccctcca
    10561 agccttccgg gtggacacct acgctacgga taagcagccg caaagcgggt aatttcctgg
    10621 aagtgcgtat ccgcgataac ggaaaaggca tatctcccga aaacatcaaa aagatattcg
    10681 atcctttctt taccacaaag cctgcaggta aaggaacagg actcggcctg tcgctcagtt
    10741 acgatattgt tgttaaggtc cacggagggc aaatacaagt tgaatcggaa ccgggcgaat
    10801 atgcagagtt catagtgaca atccccataa aacaataaga atccatggca gcagaaaaga
    10861 ttttagtggt agatgacgaa acagatctcc aatatgtaat caaccagaaa ttcaggcgac
    10921 agatccgtaa cgaccagtat gagtttcatt ttgctttcaa tgggctggaa gccttggcca
    10981 aactcataga ggtacccgat ataggggtta tcctgagcga tatcaatatg cccgagatgg
    11041 atggcctgac cctgttgagc aaggtgcgtg aattgaaaaa cccgctgctc aagactgtga
    11101 tttgttctgc ttacggcgat atggataata tccgtacggc catgaaccgg ggtgcctttg
    11161 atttcgtaac caaaccggtc gatttcaacg atttggaaat caccctggaa aaaaccatcg
    11221 gggaattgaa cattgtcagg gaaggacttg cctaccatga caaactcctc tcgattgagc
    11281 atgacctgat ggtggcacgt gaaattcaaa gggccatcat gcccaaggct atacccggtc
    11341 tggaatgggc tgatattgac ggatccatgg aagctgccag acagataggg ggcgatttct
    11401 atgattattt catgatggat gaccaccgac tgggatttgt cataggtgac gtgtcgggga
    11461 aaggcatccc ggccgctatc ttcatggccg tcagcaggac ccttatcagg gcaacggggc
    11521 tcaagggcat gtcccccagg gattgcatga actatgtcaa ccacctgttg tgtacagaaa
    11581 gcgtaaattc catgttcgta accgtgttct acggaatgct ggacctggaa acagatacgc
    11641 ttcattatgt aaatgcgggg cacaatccac ccatactgct gcgtcacaac ggtagcacag
    11701 aggtatttcc catgaccgat aacattgtac ttggcgttta tgacggttat tcgttcaaaa
    11761 ccaatagcat cccttttctc aaagacgata cgctggtaat gtatacagac ggggtgaccg
    11821 aagctttcaa ccagaataat gaggaatacg gatttgagac acttcacacg ctgcttaagg
    11881 agcaggttct cgggaataag gaggtcatgg acgggaaaga tgctccggcg cggatctgcc
    11941 tgatcatcgc tgatgatgtg aaccgttttg ccggtgaaga agagcaaagc gacgatatca
    12001 ccatcatggt tgtcaaacga aattagcaca gcctgatgaa acgcacactg gaacttgaga
    12061 atcaattatc cgccttgtcc ggtctactgg atatgacggg cgatttcctt gatgcacata
    12121 aagttccggt ccccgttcaa aataagatca aactcatcct ggacgagatc ttcagcaaca
    12181 tcgtatctta tggttacctg ccacaggatc cgcccgacac cgtccttgtt gagctgtgta
    12241 tcgcaaatgg gaaattcacc gcgatgttca acgatggggg aatgcctttt aaccccttgg
    12301 aatacggacc ggtggacacc agtcttcctc ttgaggagag acccatagga gggcttggaa
    12361 ttcaccttat cagaaacctg gccaccgaac ttgcatacga gagaaaagaa aacaggaacg
    12421 ttttttgctt ctccatagag attgaatgaa aacaaaactg accatcgcgg cattagtcct
    12481 atacctgctg gcccctctgt gcctgcaggc gcagacggct aaaacatcta aagggtatct
    12541 tatgggttac gttgtagaaa acggggatac cctgttcatc gctaccatac ccgacttgca
    12601 cgtgtacagc cgggacaaga agaaaagcga tcgcgaatgg agggaatatt acaggaccgt
    12661 tcacaatttt gcccgtgcct acccctttgc gctacaggcc agggaacgca tggatagggc
    12721 ggacagtatt ctggaagtca gcaacttctc cccaagggaa cgggaacgtt tcctggaaaa
    12781 aaccgaacgt gatctttttg ccgaatttga agcccccctc agaaaactta ctttcaccca
    12841 gggtcgtatc ctgcttcgcc tgattgaccg tgaacttggc cagacaagct atgcagtggt
    12901 gaagagttac cggggcggac tgaccgcctc gttctggcag ggggtcgcac gtattttcgg
    12961 agcagatatg aaaaagccgt acgacaagta cggcgaagac aaattacttg aagaattagt
    13021 gcgcatgtac cacgacgggc gcttttatta tctctatttc tcccttttcg gtccccctaa
    13081 agataccaat atctccccgt acgggaccag atgggccaac tgacaaccag tcttccagca
    13141 caataagttc tcctccggcc ggcagggggc agcgttccga tgtatgtatg tgcccggtaa
    13201 tgcaataatc caccgggcga tcgtatgtgg cagcatattg atagacaggc ttatacagat
    13261 tcatgtcctg tttgcacgat ccattgtgtt tatccctgtt ccaggcagac catttccgtg
    13321 caagggcaaa ggcccacctg gggtgaaggc agctgaaaag gacctgcaga agcctgttgt
    13381 gaaacattcc gtgtaagaag cggcttgcac agggctgcct gcccatgcgg tgcccgtgcg
    13441 ccagaagaaa atacttaccc tccagtttca cataccattc atcacggtgc acctgcatgc
    13501 ccagctcctg ctccaggtat ccgtatgtcc acaaatcgtg attgcccaca aaaaaatgga
    13561 ccggtatccc gcggtcatgc acccgggcaa gcgctcccag tgtgcgggtg tatcccctgg
    13621 ggatcacata cttgtattcg taccagaaat caaagatatc cccaagcaga aaaagccctc
    13681 ccgtcccggg agcgaggctg tccaggaaag cgataaagcg tgcttccctg tccgacgggt
    13741 ctccgttcct gagtcccaaa tgaatatctg caacaaaata ataagtgctc acaacaatgg
    13801 tacgataagg caggcaagtc ctgccaacat gcaatatacg gcaaaccagg taaacctgac
    13861 tttgcgcaca atggttatca taagtttgca ggccagcaat ccggaaacaa aggcagctgc
    13921 aaatcctatg gccagtgcca gaaatgtaat ccctgtccgg gccggggcaa aacctcctcc
    13981 gaggatctca agaaaagctt ctcccaggac agggatcaac accatcagga aagagaatgg
    14041 tgccaccttg tctttatttc ctcccagcga gagaccggtc gctatggtcg ctccggaacg
    14101 cgacaacccg ggtaagacgg caaatgcctg ggcaatccct atcaggaaag cttccctgta
    14161 accaatggga cgaaccgagc ctttcttgtg tctgagtgcc gagagccact gggaaaccat
    14221 cagcaacacc gctgtcagca acaacatcca tcccactatg gtcagtcccc cggaaaaaag
    14281 tgtctcaacc ttttccttga aaaacaatcc gacaataagc acagggattg ccgaaaataa
    14341 aaggcggagt gtaagcccgg tcgcaggggt gttccgaaaa cgcagggtgt cagaaaccat
    14401 ttcccacaac tctttccgaa acaccacaat agtactcaag acagtggcca gatggaccac
    14461 aatctcgaat gaagcatcct cggtttcaat gccaaacagt gtctttacaa taacaagatg
    14521 accgctgctg ctgaccggaa gaaattcggt caaaccctgc aacaggccca ggataagcgc
    14581 ctcaaaccct ttcattcccc ttatccttct taacgtgcat gatggcgatt atctcaaaga
    14641 caaagccacc gcaaatcagt aggggagcta taaccatccg gcgaaaacta aacaaggcct
    14701 ggcccgtaaa taccccggga tcgtcagagc cgcccccggc caataaaaca aaacccagtg
    14761 ctatgaccac caggccaata agcaaaaggt acacgttcct tgccggaagg gcaaatttac
    14821 cttttgtttc ctgttcattc tttttttctt tcttcatttc atatcaataa taaaaggcag
    14881 aagactcaag cttcaccaca cggtttacga caaaaaaggt ggatatcctg cacagcagta
    14941 tcccggtcag gatcatggca cccatcacaa tcaggaacat ctcccgggat acaagcatca
    15001 gaatgcctgg aaattccctt tgcagcatat atagtatacc tatcagcaat acaatggcaa
    15061 taagcgaaga caggaacccc tgtattaagg cctgtgtcat gaaaggctta cggatgaaac
    15121 gtcttgtggc acccaccagc ctcatggcat atatggtaaa gcggcgggca aagacagata
    15181 atctgatggt attgtgtatc agcgcaaatg aaatgatcaa aagaacgaga atgatgactc
    15241 ccaggacgac gccaatatta cgtaaatttc tgtttattaa cgtaatgagg ggtgcctggt
    15301 atgatacttc ctcaacatac aggagtttta acaggtatgt ttccatgcgg gctatactgt
    15361 cgttgtttac ataatccgca ttaagcctca gttctacaga tgcgggcagg ggattgtact
    15421 caaagatgtc caggaattcg ctgcccagca actgggccat ttctgcagca cccttttcgc
    15481 gcgaaatgaa aactgcctcc cgtacagcat cccttacata gggcgttgca agaaactgtt
    15541 tgattatcag tgtggaatct gcatcctttg tttccatgtc aagaatgaca gaaaccccaa
    15601 gatgttcctt gaaataatcc gagaccgtcc tggcgttgac agcgagcaac cctgtaatgc
    15661 ctatcaggag caacaccagg gatatactta caacagatga cagataggac tgtacaagct
    15721 gttggttgat aatatttttc tcctgttgcg ccatcgttag tggttccaaa gataaagaaa
    15781 tataagaaat ttttgctatc tttgtatgat ttcaagccct caagcacaat gataaaagcc
    15841 aaaaagatcc tttatgtgag ttcggaaatt tttccttttc tcccacaaac ggatatgtct
    15901 tatgttggcc gtcatttacc ccaggctatc caggaatctg gcggtgagat ccgttctttc
    15961 atgccaaaat acggatgcat caatgagcgc cgcaaccagc ttcatgaagt gatacgcctt
    16021 tcgggcatga acatcattat caaagacatt gaccgtccac tgattatcaa ggtggcctcc
    16081 atatctacgg cacggatgca ggtatatttc attgacaacg aagattattt ccagagaaaa
    16141 agcacataca gagacgcgaa tggtgagttt ttcgctgaca acgatgaacg gggcatattt
    16201 tttgctcggg gtgtactgga aacggtgaaa aagcttcgct ggaagcccac cctggtacat
    16261 tgtcacggat ggctgagcca cctgttgcct ttgttcctga aaaaagcgta ccacgacgat
    16321 ccgcttttca ccaatgtccg cgtggttgtc tcgttataca acgacctgac cacagaaagt
    16381 tttaacgaaa aaatgcggtc caaaataatt atgcccggca taaaagcaaa agatatggat
    16441 tttctggaag aacctgatgc cgtcaatctt gccaaaacag cggtacaata tgcccacggt
    16501 gtcatcctgg cccatcccgg agtagacagc tctatcgcca aatatgccgg atcccttaat
    16561 attcccgtca tgccatacat tgatccgcag gaccctaaga gcaactacat aaaggagtat
    16621 aacaactttt acgatcaaat tttagaaacc caatgacaat cagggcttgc ggcattatcc
    16681 tgtctgcgtc tctcctattc ttgatttcct cttgcattac ggtggataac cgcatgggag
    16741 ccgatttcat ccctacaaac cagatattgc atatcaactc tgtggagctg gaggccccca
    16801 tgttcactgc ggtggcagac agcatgaata tgtcatatcc tgcttacctg gcctttggat
    16861 ccgttaactt cccggtattt ggcaagacaa ccacttccgc actggtccag ttcgcaccat
    16921 attcctatca gaaagaatat ggcgctgacc ccatagtatc cgatatgttc gtgaatattc
    16981 cgctgggcgg atttaccgtc tctggtgata atgaccgtta cataccccaa aacgtatatg
    17041 tttacaagat cataaaagac ctggataaac tggatgtata ccacaaatcc ttttcggagg
    17101 agttcataga tccgatcccc gtaagcaaac cgggacagat tttcttggga cgggatacct
    17161 tgcgcataga gttcacagaa gagttcgcct acgaactgct ccaggcagat tccattgaaa
    17221 gggacagcac agaagctttt attgaaagat tcaaggggct gtacattacc acagacgacc
    17281 tgaacacatc agaaacggga ggcaggatga atttcctgga catcacggaa gccaatatct
    17341 atctgaacta caaatcagga cagggtgata gcctgttaac ctattttttc aacatatacg
    17401 gcacctatta caatgtttct tcccaccagt cctctcatct tgccacgaac tcgcctgctg
    17461 cacacctgta ttacgaaggc cttgcaggag taaaaccctg cctggatgcc tccaatatga
    17521 gagaccgtat caaaacctgg ggattggccc agacacctcc tgtagacgcc agccagattc
    17581 tgatttcccg tgccgatctg gtactgcccg ttgagatacc gttaaacaat gattattcca
    17641 ccgtagacaa agctcctggg aatttatttc ccagtcgcct ggtatccaac gataccctta
    17701 ccttctacag cccgctgaat gaaatttact accagaattc cggcgggaca atgaatcgtt
    17761 cccgctggga gtacactttt gaaatcacca catacctgca ggatttgctc aacaaagaca
    17821 ccataacctc acgtgataac ctgtggatca tgcccaccta tgttgtttca ggcacttcag
    17881 cagacacgta ctacattgac aacgcaactt accgcaccat gattcttaac ggcaacctgt
    17941 cagacaggcc tccttatgta aggataacct atgccgtgtt gttacaataa aaaaaatcgt
    18001 tacatttgca ccccgtttta aagacttcgt agctcagctg gtagagcaaa tgactcttaa
    18061 tcattgggtc gagggttcga atccctccga ggtcacaaca tgc
//
LOCUS       DAOSOF010000024        17905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3171_length_17905_cov_4.807507, whole genome shotgun sequence.
ACCESSION   DAOSOF010000024 DAOSOF010000000
VERSION     DAOSOF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17905
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3171_length_17905_cov_4.807507"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="PLM68_02550"
     CDS             <1..153
                     /locus_tag="PLM68_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOT54279.1"
                     /translation="LDNSKFSQDKWPVAYDLLLNCGGEEKAGFIGLQDHGDDVWFRNI
                     QVKILD"
     gene            153..1022
                     /locus_tag="PLM68_02555"
     CDS             153..1022
                     /locus_tag="PLM68_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT54280.1"
                     /translation="MKSNFIMLAMAAFLGLASCAQEPVQKEIGLQLYSIRNEISQDLE
                     TALDAVAAAGYTFVEMAGYNAGDGTFYGLAGDAFTALCNSKGLDVISSHINGPDPNAA
                     TWEECMAWWDKAIQDHLAAGVLYIVQPSMWMSAYESVEGLARYCELFNAVGEKCNQAG
                     IRFGYHNHDKEFTTVFDDMVMYDFMLANTDPEKVFFQIDVYWSQVGGAPATDYFEKYP
                     GRFDLWHVKDEKEIGASGTIDFQALYEKSEIAGLKYAIVEQEAFDLEPFESIKASYDF
                     LKNAPYVKDTYSN"
     gene            complement(1058..3052)
                     /locus_tag="PLM68_02560"
     CDS             complement(1058..3052)
                     /locus_tag="PLM68_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54281.1"
                     /translation="MAQRTRLHILLLILVFITPGKALAQYDKQQFFYRGQQLLFEGRY
                     QQAIDNFNKLIQLDPELHEAYFFRGIGKYNLGDFLGARTDFDKALAIHPIFTLAYHYR
                     AITHSRLGDYEKALADLQEAIDLRPGYNGLYFSRGVTYLLSQQFKLAEQDFNRFIRHE
                     PRVSEAYLNRGACYLYLKDTVKALHDYDTAILLNKFDPEGYIRRSRVYAMQENYAAAL
                     EDLNQALSLEPDNSFAYFNRALIRFNQNDLTGALQDLERVLEEDPGNALTLYNRALIR
                     TQIGDYNNALSDYDRVISINPGNVLAYYNRAALFIQMERYGDALRDYDKAIELYPDFA
                     NAYMNRSYVKNRMGLFAAAEVDYKTAQSKVNQYKSQLGDSTYSAFADTTQRFDRLLAL
                     DADFARKDFDNELLQYRDVDIRLKPLFKLLTGTSGNLIALERRYYYEELEKFLLKQSV
                     PVYLFSEEPVVDPNQLSFLREQVRSLLQQSDDSPDLYFISGILDFQSKLFNASLLSYN
                     QAISRDPDNVFYYINRAALQSEMIEFISSMESNVQILTLDESGTTRTRVQEQGRREYD
                     YSGAIDDLEKAAMLAPDFAHIYYNLGNLYCLSGDFPKSIGNYTRSIELYPYLAEAYYN
                     RGLIQIYLKDKEKGCMDISTAGELGIKDAYSVIKKFCVTE"
     gene            3114..4103
                     /locus_tag="PLM68_02565"
     CDS             3114..4103
                     /locus_tag="PLM68_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT54282.1"
                     /translation="MPILLLFALFLGIAPSFAGQSSQTPLLRPEYHAGLINRKELNTH
                     ICYLSDSSLQGRETGTAGGSAATSYIAGKFMEYGLRPLGTGNPQRNNTSFIQGFACPT
                     GQRGRNVIGWIPAATHSDEYLVVTAHYDHLGMINGIFYPGADSNASGVSVLLSLARAF
                     CALENEGYRAARNIIFVAYDAKEFSMTGARVFALSLGIPAKSIVLNMNLDQLGTSLEP
                     PGSTSQYVLVLGAGNVPSYIVRSLDNNNSFFNMGLNIDYSFYNSPDFARIYFTMTEQS
                     VLAAMEVPSLLITSGFNDHTYKPTDTPEIIDLPVLEKRARWIFFSLWDMAYRR"
     gene            complement(4091..4858)
                     /locus_tag="PLM68_02570"
     CDS             complement(4091..4858)
                     /locus_tag="PLM68_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT54283.1"
                     /translation="MIRIPFMPIVLGIVFCGMPLQNLKAQESEAFTSLFCIAPQIGTD
                     IGGTIPIPFSAIGGPFNAYPKLNATLGMRFFLNIHPRWSLGANLNYKTIAMDADARVT
                     NQKFKGENTVQYFTGTSEMSMSFQILELPLYVEFLAGSKQQHGIQVGAFAAWVMKSHF
                     ITYANKGFIGSEPDRMDSALSSPQVMDFSALLGHWDAGLLAGYEARIFPHIRMGLHVM
                     FGLNDIFIPGSDFFDYSMKQVRGSVTVSYDLVRLYRR"
     gene            complement(4897..7689)
                     /gene="uvrA"
                     /locus_tag="PLM68_02575"
     CDS             complement(4897..7689)
                     /gene="uvrA"
                     /locus_tag="PLM68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT54284.1"
                     /translation="MDEEKIVVKGARVHNLKDISLDIPRDKLIVITGVSGSGKSSLAF
                     DTLFAEGQRRYVESLSAYARQFLGRIKKPDVDSIEGIPPTIAIEQRVNTRNPRSTLGT
                     TTEIYDYLRLLYARIGRTYSPVTGEEVRCHTQEDVLQYIVSLPPGTSAMILAPFGWED
                     AQRRVENLLMLQQEGYTRYHDGTEVVRTEDLLRDMEKYASRNVLLLTGRVGTADDPDN
                     RARILESVRSAFTMGQGALFVLTGEEMVAFSKRFEADGITFMEPTEHLFSFNSPLGAC
                     PECGGFGQVTGIDELLVIPDRTLSVYQHAVACWRGDVMGAYRDEVIRYASRAGFPVHK
                     PYSGLSAQEREWLWNGDEHVTGINRFFKKLESARYKVQNRILISRYTGKTLCPACGGT
                     RLRKESSYVKIKGKRIDQLLDMNVEELEGFFNAATWDKQELEIAKRTLQEITSRLRFL
                     NQTGLGYLTLNRRSSTLSGGESQRVNLVSSLGSSLVGSLYILDEPSIGLHPRDTLRLL
                     SSLKALKDLGNTVLVVEHDEEIMRAADQIIDIGPLAGRDGGKLVYQGPPLQPGQACDP
                     GDSYTLRFLSNPDVIPVPTHRLGWNSYISVEGATHNNLKGIDVKFPLQVFTAVTGVSG
                     SGKSSLVRDVLYYALGREYHQSRIPAPGLFSRLTGDVHRIGGVELVDQNPIGKSSRSN
                     PVTYIKAYDDIRKLFSEQPYARMNGYGHSHFSFNIEGGRCPECQGEGVVKVEMQFMAD
                     VTLTCESCQGKRFQPDILEVRYKERNIYDVLEMTVREAIDFFSSHDDPTARRIVERLM
                     PLDQVGLSYIKLGQSSSSLSGGESQRVKLAYYLSRENTMTNLLFIFDEPTTGLHVYDI
                     DKLLKSFSALISKGHTVLVVEHNMDVVKCADWVIDLGPGAGDKGGTCCYCGTPEGLAQ
                     RPDLPTGLALREKIRV"
     gene            complement(7694..9787)
                     /locus_tag="PLM68_02580"
     CDS             complement(7694..9787)
                     /locus_tag="PLM68_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOT54285.1"
                     /translation="MNQTMIDKAKEYAAARLEGLKRGNGAPFMQHVDAVAAIVEKEIG
                     LAGEAVATVYLHEASRKHPELLAEIASLFGHETGSMVVGLNKISTINPRDTRLQAENY
                     RKLIISYSADPRVTLIKLADRLEVMRSLADCFPRNDHIKKATETMMLYAPLAHQLGLY
                     RIKSELEDLSFKVIQPEDYRLITNNLRANEIERKKQIAGFVQPLEKELRKAGIRYELK
                     SRTKTTYSIWRKMQIQQVGVDEVYDILAIRFIVDTTPDREKEVCWQVYSIVTQIYEPI
                     PERMRDWITVPKASGYESLHITVRTPEGAALEVQIRSRRMDIVAEFGVAAHWAYKGVK
                     NEQVMNDWLAGVRKRLQNPQEDFSSAEKDLYKSNEIYVFTPAGDLRRLPAGATVLDFA
                     FEIHSNIGLRCTGGRVNGKMSQVRDTLQTGDVVEILTSRNQKPSRDWLGFVVSGKARN
                     KIRQKLREEEGIQDVAGREMLERRLKNWKMELTPEDLMQLMKHFRIKQISDFYAAVAR
                     EDISLQDIKLFLLKDKEDTSEKPETGSTPPEHAEEKTSYREGDYLVIDDNFGRIGYKL
                     ARCCNPVIGDEVFGFVSIKEGIKIHRMSCPNAARLMTRYPYRIQKVRWREKVTSAMFQ
                     ITIRFTAYQEIGLAQEISDVIGNLSLNLRSFTFSGQGGKIAGKLQVAVSSNKQVDLLL
                     FQLRKIRGITKAVRE"
     gene            complement(9784..11775)
                     /gene="uvrB"
                     /locus_tag="PLM68_02585"
     CDS             complement(9784..11775)
                     /gene="uvrB"
                     /locus_tag="PLM68_02585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT54286.1"
                     /translation="MQFKLTAPYAPTGDQPQAIEQLASLLAEGTEAAVLLGVTGSGKT
                     FTMANVVERLQRPTLVISHNKTLAAQLYAEFKSFFPENAVEYFVSYYDYYQPEAYLPV
                     TDVYIEKDLSINDEIEKLRLSTSSSLLSGRRDVLVVSSVSCLYGIGNPEDFHANTITL
                     KTGELHDFNKLLYRLVDALYSRNENEFKRGTFRVRGDVVDIYLAYDDIACRISFWGDR
                     IDSIERVDPMTGGVIERIEGIAIYPASAFVTTKERKEQAIRSIQDDLTERVATLKELG
                     KHLEARRLLERVEFDIEMIRELGYCPGIENYSRYFDGRPPGTRPFCLLDYFPDDFLTI
                     IDESHVTLPQIRAMFGGDRSRKEVLIEYGFRLPAAADNRPLTFAEFDKVIGQRFYVSA
                     TPADYEMERSCGLIVEQVVRPTGLLDPPVEVRKTEGQIDDLIEEIRSRAAKDQRVLVT
                     TLTKRMAEELDKYLQKIRIRCTYIHSDVDTLERIRILEEFQKGYYDVLVGVNLLREGL
                     DLPQVSLVAILDADKEGFLRSERALTQTAGRAARNVDGRVIMYADKITDSMARTIEET
                     DRRRNKQMQYNRENGITPTQIALNIRRTMSQEPAYSEAPDVISMVAEDPVARAMTPQQ
                     LEKAIATARKRMEEAATKLDFITAARFRDEMYGLEKLRK"
     gene            complement(11812..12339)
                     /locus_tag="PLM68_02590"
     CDS             complement(11812..12339)
                     /locus_tag="PLM68_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT54287.1"
                     /translation="MIYLELFYTFFKIGVFCFGGGYAMLSIIQDEVVRNNGWMTMESF
                     SDIVAVSQMTPGPVAINTATYVGYTASGGNVLGSFMATIALIIPSVVMMSVVIRVYRK
                     YSQNKTFQAIFSGIRPVVAGLIAAAVLNMATPENFVDFRSYLLFSASFLAAYFYRAHP
                     ILLLALGGLAGYLMY"
     gene            complement(12336..12887)
                     /locus_tag="PLM68_02595"
     CDS             complement(12336..12887)
                     /locus_tag="PLM68_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT54288.1"
                     /translation="MYWELFKVFFRIGAFTIGGGLAMLPVIEREVVDRKKWITKTVFT
                     DIVAVTQTLPGVIAVNMAVAVGYKVKGTRGSLVSVLGAVCAPFFSILALAIFFQNFRD
                     NPVVEKIFLGMRPVVVALIAVPVITTMRSIGLTYATGLIACVATLLIWLLGVSPVWII
                     LAAGLGGYAWYTIDKHRKRRRST"
     gene            complement(12890..14158)
                     /gene="mtaB"
                     /locus_tag="PLM68_02600"
     CDS             complement(12890..14158)
                     /gene="mtaB"
                     /locus_tag="PLM68_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT54289.1"
                     /translation="MNEACTFTVITLGCKLNYAESATMARDLLHRGYQRVGLSRGADI
                     TIINTCSVTEHADKKSRQAIRKALHINPKTQIIVTGCSAQLRAESLRLIPGVETVLGT
                     DFRDRVGEVAGNPGVGALFPGTGTVGSVFFPAWSGGEDHRERTRSFLKVQDGCDYCCT
                     YCTVPLARGRSRNIPIAEVVLQAREIAGRGVKEIVLTGVNTGDFGKTTGESFADLLRQ
                     LSYVEGIERYRISSIEPNLLTGEIIEMTARLKCFLPHFHIPLQSGSSRILRKMGRRYD
                     PELFAGKVSHIRSLMPFAFIGVDVIAGFPGETEADFMETHNLLATLKPSFLHVFPFSP
                     RPGTPAAKLPMSERIREGIITERTRMLGQLSDKLHQEFILANKGRKEEVLFEGKTGND
                     KMHGYTRNYIRIERPYDRSLINTVVEIILD"
     gene            complement(14151..14519)
                     /gene="folB"
                     /locus_tag="PLM68_02605"
     CDS             complement(14151..14519)
                     /gene="folB"
                     /locus_tag="PLM68_02605"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOT54290.1"
                     /translation="MATIALKDLRFYSHHGCFDEERLIGTFFSVDVELSIHGCPAAFT
                     DKLEDAVDYSRVYELVSAEMSIPSRLLEHVAGRILRSLCRSFSLSDCRVKVSKLSPAL
                     GGGEVGSASVTLTMKDLQHE"
     gene            complement(14523..15236)
                     /locus_tag="PLM68_02610"
     CDS             complement(14523..15236)
                     /locus_tag="PLM68_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT54291.1"
                     /translation="MNKPALYLVPAPLGDTTPSGVLPADHVEMVRKLDCFIVEEPRTA
                     RRYLSALGLDVRSLELEVLNEHTSPAELNGLLEPLRKGRSVGLLSEAGLPAVADPGAE
                     LVALAHRGGFQVIPLVGPSSLMLALMASGLNGQKFAFQGYLPVKSGLRDKRIRALERQ
                     SAQENQTQIFIETPYRNESLFRALLDCCAPGTRLAVAINLTLPDAFICTRTVGEWKGK
                     EPDFSAAYRKKPCVFLLQT"
     gene            complement(15233..16288)
                     /gene="mltG"
                     /locus_tag="PLM68_02615"
     CDS             complement(15233..16288)
                     /gene="mltG"
                     /locus_tag="PLM68_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT54292.1"
                     /translation="MRKKSRFFLLFIILTLGAGIVAGGWYYLTFHYPNITTEDGKTAY
                     LLVPRGTTYEQLIDSLRSGGHLTMEQTFRCAAKHENLPENVHAGRYALTHGMNNRELA
                     RKLAMGWQDPLNITINAPIRTFERLASVLARNLEIDSALVLQHLRNDSLIATFGFDSV
                     SLRGMFIPNTYQVYWTISGEDLLERMSREYNSFWNEQRKEKARSIGLTIRQVSTLASI
                     VNEETKKTDEMPVVAGVYMNRLRIGMPLQADPTLIFAAQDYSIRRVLARHKRIESPYN
                     TYKYKGLPPGPIAVPSIEAIDAVLNYDHHDYLYFCAKEDLSGYHNFALNLRDHMKNAR
                     RFQRALTERLNKEVKAQ"
     gene            complement(16299..17285)
                     /locus_tag="PLM68_02620"
     CDS             complement(16299..17285)
                     /locus_tag="PLM68_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOT54293.1"
                     /translation="MLNPWLYKAFCFVSCILPVLVPAKASPWSEDPERQITREEYINR
                     YKNIAISQMYHYGIPASIILAQGCLESGNGNSRLAREGNNHFGIKCHDWEGDRIYHDD
                     DALQECFRKYRTAEDSYYDHSEFLRTRPRYASLFLLERTDYASWARGLKQSGYATNPR
                     YDELLVELIEKYSLHQYDLLPLQEEEGREPLAMKSGIPAAGMPSYAMTGEDVFVIDVE
                     RRILQTNGKRYVLSAPGDTYRSLAAEFGFFRSELTRYNDVAANAELAVGEKVYLERKA
                     GRVTRGECSWSSLSGESLQEISQRFGIRLQSLRTLNPGTGDYPLAGTVIRLR"
     gene            17338..17775
                     /locus_tag="PLM68_02625"
     CDS             17338..17775
                     /locus_tag="PLM68_02625"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT54294.1"
                     /translation="MNRSILTIDYTETTKDLLDPLDAELLDKAARAADGAYAPYSRFL
                     VGAALRLENGQVICGNNQENASFPAGICAERTAVFYAHANFPETQIRAIAITASPCGI
                     CRQVLLESEKRAGVPIRVLLAAGDKVRIFQSVKDLLPFGFDSF"
     gene            17795..17894
                     /gene="ffs"
                     /locus_tag="PLM68_02630"
     ncRNA           17795..17894
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLM68_02630"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         4208 a         4827 c         4281 g         4589 t
ORIGIN      
        1 ctggataaca gcaaattcag ccaggataaa tggcccgttg cctacgatct gcttctgaat
       61 tgcggaggag aggagaaagc aggtttcatt ggtttgcagg accatggcga cgatgtatgg
      121 ttcaggaaca ttcaagttaa aattctggac tgatgaaaag taattttatc atgctggcca
      181 tggcagcctt cctggggctg gcttcctgtg cccaggaacc tgttcagaag gaaatcgggc
      241 tacagcttta ttccattcgc aacgagataa gccaggacct ggaaacagcc ttggacgctg
      301 tcgctgccgc cggctacact tttgtggaaa tggccggata caatgcaggg gatgggactt
      361 tttacggcct tgccggggat gccttcaccg ctttgtgcaa ttcaaaaggg ctggatgtga
      421 tctcatcgca tatcaacggc cccgacccca acgccgcaac atgggaagag tgcatggcct
      481 ggtgggataa agccatccag gatcacctgg ctgcaggcgt gttgtatatt gtacagccct
      541 ccatgtggat gagcgcctac gaatctgtgg aaggccttgc ccgttattgt gagctgttca
      601 acgctgtggg cgaaaaatgc aaccaggcag gcatccgttt tggttatcat aaccatgaca
      661 aggaattcac tactgttttc gatgacatgg taatgtacga cttcatgctg gcaaatacag
      721 acccggaaaa ggtcttcttc cagatagacg tttactggag ccaggttggc ggtgcccctg
      781 ccacagacta ttttgaaaaa tacccgggac gttttgacct ctggcacgtg aaggacgaga
      841 aagaaattgg agcttcggga accattgatt tccaggccct ttatgaaaag tccgaaattg
      901 ctggtctgaa atatgcaatt gtggagcagg aagcattcga ccttgaaccc tttgaaagca
      961 tcaaagcctc ctatgatttc ctgaagaacg caccctatgt gaaagacacg tattccaact
     1021 gagcaggaag gtaccggtaa atgaagattc tgtgtcgcta ttcagtgaca cagaattttt
     1081 ttatgacact gtaagcatcc ttgataccca gttcccctgc cgttgagata tccatgcatc
     1141 ctttttcctt gtccttgagg taaatctgga tcagtcccct gttataatag gcttctgcca
     1201 ggtaagggta cagttctatg gaacgggtat aatttcctat ggatttggga aaatccccgg
     1261 acaggcagta caggtttccc aggttgtaat agatatgggc aaaatcagga gccagcatgg
     1321 ccgccttttc caggtcatct atagcccccg aataatcata ttcacgtcgt ccctgttcct
     1381 ggacacgggt tcttgttgtc cccgattcat ccagtgtcag tatctgcacg ttgctttcca
     1441 tggaagaaat gaattcaatc atctcactct gcaatgccgc cctgttgata tagtaaaaga
     1501 cgttgtccgg atcacggctg atagcctggt tgtaggacaa aagggaagca ttgaacaatt
     1561 tgctttggaa atccagtatt ccactgataa agtacagatc cggggaatca tctgattgtt
     1621 gaagcaggga acgtacctgt tccctgagga agctaagctg gttcgggtcc acaaccggtt
     1681 cctcgctgaa aagatagacc ggtacggatt gtttgagcag gaatttttcc agttcttcat
     1741 aataatatct tctttccagg gctatgaggt ttcccgaagt accggtcagg agtttaaaga
     1801 gtggtttcag acgaatatcc acatccctgt attgcagcaa ctcgttatcg aagtctttcc
     1861 tggcaaaatc ggcatccagg gccagcaggc ggtcaaaacg ctgcgttgtg tctgcaaagg
     1921 ctgaatatgt ggaatctccc agttgtgact tgtattgatt gaccttgctt tgtgctgttt
     1981 tgtaatccac ttctgccgct gcaaacaggc ccatcctgtt tttgacgtag gaccggttca
     2041 tatatgcatt ggcaaaatcg gggtataatt caatcgcttt atcatagtcc cgtaatgcat
     2101 ccccgtatct ttccatctgt atgaaaaggg cggccctgtt gtaataagcc aggacattgc
     2161 cgggattgat ggatatcacc cggtcataat cgctcagggc attgttgtaa tcgccaatct
     2221 gtgtacggat caaagccctg ttgtataggg taagcgcatt accgggatct tcctccaaaa
     2281 cccgctccag gtcctgcagc gctcctgtca gatcgttctg attgaagcgg atcagtgccc
     2341 ggttgaaata ggcaaacgaa ttatccggct ccagggatag ggcctggttc aggtcttcca
     2401 gcgccgcggc ataattctcc tgcatggcat acaccctgga ccttctgatg tacccttcgg
     2461 gatcaaactt gttcagcagt atggctgtgt cataatcgtg cagggctttt accgtatcct
     2521 ttaaatacaa atagcaggcc cctctgttca ggtaggcttc gcttaccctg ggttcatggc
     2581 ggataaagcg attgaaatcc tgctctgcca gtttgaattg ctgagacaat aagtaagtca
     2641 ctcccctgct gaaatagagt ccgttgtatc ccggcctgag gtcaatggcc tcttgcaggt
     2701 ccgccagcgc tttttcatag tcacccaggc gggaatgtgt tatggcacgg taatggtagg
     2761 caagcgtaaa aattggatgg atggccaggg ctttgtcaaa atctgtcctg gctcccagga
     2821 aatctcccag gttatacttg cctatccccc tgaaaaagta agcttcgtgg agttccgggt
     2881 ccagctgtat cagtttgttg aagttgtcaa ttgcctgttg atagcgtcct tcgaacaaca
     2941 gttgttgtcc cctgtaaaaa aactgttgtt tatcgtattg ggccagtgct tttccaggcg
     3001 taatgaaaac caggatcaat aaaaggatat ggaggcgtgt tctttgtgcc attgttcaaa
     3061 gatatgaaat tgacaggaaa attcttttct ttgcagctta aatgacaaaa aatatgccga
     3121 ttctccttct gtttgcgctg tttctgggca tagccccctc ttttgccggg cagtcttcac
     3181 agactcctct cctgcggccg gaatatcatg ccggtctgat caaccggaag gaactcaaca
     3241 cccatatctg ctatctttcg gacagctctc tccagggaag ggaaacaggt acagccggag
     3301 gctcggcggc cacaagctac atagccggaa aattcatgga atacggcctg cgccccctgg
     3361 gcaccggcaa tccgcaacgc aacaatactt cctttatcca gggttttgcc tgccccacag
     3421 ggcagcgggg caggaatgtc atcggctgga tccctgctgc tacccatagt gatgaatacc
     3481 tggttgttac tgcacattac gaccacctgg ggatgatcaa cgggattttt tatccgggtg
     3541 ccgacagcaa tgcttcgggt gtttccgtac tgctatccct tgcccgtgca ttctgcgccc
     3601 tggaaaatga agggtaccgg gccgcacgga atatcatttt tgtagcttat gatgcaaagg
     3661 aattctccat gacaggtgcg cgggtctttg ccctttccct gggaataccc gccaaatcca
     3721 ttgtattgaa tatgaatttg gatcagttgg gtaccagcct ggaaccaccg ggcagcacgt
     3781 cacaatatgt gctagtgctc ggggccggaa acgttccctc gtatatagtc cggtcattgg
     3841 acaacaacaa ttcttttttc aacatgggac tcaacataga ttattctttc tacaacagtc
     3901 cggactttgc cagaatctat tttaccatga cagaacagtc cgtgcttgca gccatggaag
     3961 tcccttcact gctgataacc tccggtttca acgatcacac ttacaaaccc acggatacac
     4021 cggagatcat agacctgccc gtactcgaaa aaagggccag gtggattttt ttcagcttgt
     4081 gggatatggc ctatcgccga taaagacgca ccaggtcgta agaaacagtc acgcttcccc
     4141 gtacctgctt catgctgtaa tcaaagaaat cgcttcccgg gataaagata tcattcaagc
     4201 cgaacatgac gtgcaagccc attcttatat ggggaaatat ccttgcttca tagccggcca
     4261 gcagtcctgc atcccagtga cccagcaacg cagaaaagtc catgacctgg gggcttgaaa
     4321 gggcagaatc catccggtcc ggttcagaac cgataaaacc tttgttggca taagtgataa
     4381 aatgagactt catcacccat gccgcaaaag ctcccacctg gataccatgc tgttgtttcg
     4441 atcctgccag gaattcaacg tacaggggaa gctccagtat ttgaaagctc atggacattt
     4501 cagaggtacc cgtgaaatat tggaccgtat tctctccctt gaatttctgg ttcgtgacac
     4561 gggcatccgc gtccatggct atggtcttgt aattcagatt ggcacccaag ctccagcggg
     4621 gatgaatgtt taaaaaaaag cgcattccca gcgtggcgtt cagtttagga tacgcattaa
     4681 aaggccctcc tatggcagag aaagggattg gaatagttcc ccctatgtca gtgccaatct
     4741 gcggggcaat gcaaaataag gaagtgaaag cttcggattc ctgtgccttc aggttttgca
     4801 ggggcattcc gcagaacact attcccaaca ctatcggcat gaaaggaatt ctgatcatat
     4861 cacaaaaata gaaacttctc ccgttcccgg tacccgttac acccttattt tttctcgcaa
     4921 ggcaaggccc gtcggcagat cgggcctttg tgccaagcct tcgggtgttc cgcaatagca
     4981 acatgttccg cccttatcac ctgctcccgg accgaggtct atgacccagt ctgcacattt
     5041 gaccacatcc atgttgtgtt ccacgaccag aaccgtatgc ccctttgaaa tcaacgccga
     5101 aaaggatttc agcagtttgt cgatatcgta gacatgcagt cctgtggtag gttcgtcaaa
     5161 tatgaacagg agattggtca tggtgttttc cctggacagg taataagcca gcttcacccg
     5221 ctggctttcc ccgccgctca ggctgctgct gctttgaccc agcttgatat aggacaaacc
     5281 tacctggtca aggggcatga gcctttcgac tatacgacgg gcagtgggat cgtcatggga
     5341 agaaaaaaag tctatcgctt cgcggactgt catttccagc acatcgtata tattcctttc
     5401 cttgtacctg acttccagga tatcgggctg gaagcgtttg ccctggcatg actcacaggt
     5461 cagggtcaca tccgccatga attgcatctc taccttgaca acaccctctc cctgacattc
     5521 ggggcagcgt ccgccctcaa tattgaaaga aaaatgagaa tgtccgtaac cgttcatgcg
     5581 tgcgtacggt tgttcggaaa acagctttct tatatcgtcg taagccttga tataggtaac
     5641 cggatttgac cgggaagatt ttccgattgg attctggtca accagttcca cgcctcctat
     5701 ccggtgcacg tcccctgtca ggcggctgaa caacccgggt gccggaatcc tgctctgatg
     5761 gtattccctg cccagggcat aatacaacac atccctcacc agggaagatt tgcccgatcc
     5821 gctaaccccc gtaacggctg taaatacctg cagtgggaac ttcacatcga ttcccttcag
     5881 gttgttatga gtcgctcctt caacggatat ataggaattc catcccaacc ggtgtgtggg
     5941 caccgggata acgtcagggt tggaaaggaa acggagggta tagctatctc ccggatcaca
     6001 ggcttgcccg ggctgcaagg gaggtccctg gtatacaagt ttgcccccgt ccctgcccgc
     6061 cagcggtcca atatcgatga tctggtcagc cgcgcgcatg atctcctcgt catgttccac
     6121 aaccagcact gtgttgccca ggtctttcag ggctttgagc gaggacaaaa gcctcagggt
     6181 atcccgggga tgcaggccga tgctcggctc gtcaagaatg tacaggctgc ctaccagcga
     6241 gcttcccagg gagcttacca ggttgacacg ctgactttca cctccgctca acgtggaaga
     6301 cctccggttc agtgtcaggt aaccaagccc cgtctggttc aggaagcgca ggcgggacgt
     6361 gatttcctgc aacgtccttt tagcaatctc cagctcctgc ttgtcccatg tggctgcatt
     6421 gaaaaagccc tccagttctt caacattcat gtccagcaac tggtctatgc gcttgccttt
     6481 tattttcacg taagatgatt ctttacgcaa cctggttccg ccgcatgcgg gacatagtgt
     6541 tttcccggta tagcggctta tcagtatcct gttctggact ttgtaccggg ccgattccag
     6601 ttttttgaag aaccggttga tgcccgtgac gtgttcatcc ccgttccaca gccattccct
     6661 ctcttgggca gaaagaccgc tatagggctt gtgaacgggg aatcctgccc tggaagcata
     6721 cctgatcact tcatcccggt aggcacccat gacatcaccc ctccagcagg caacggcatg
     6781 ctggtagaca gacagggtcc ggtccggaat gaccagcaat tcatctatgc ctgtcacctg
     6841 cccgaagccg ccacattcgg gacaagcccc caacgggctg ttgaagctga aaagatgctc
     6901 ggtgggctcc atgaaggtta ttccatcggc ttcaaaacgt ttggaaaaag ccaccatttc
     6961 ctctcctgta aggacaaaaa gcgccccctg gcccatggtg aaggctgacc gtaccgattc
     7021 cagtatacgt gccctgttat caggatcatc cgcagtcccc acccgtccgg tcagcagaag
     7081 gacattccgc gatgcatatt tttccatatc cctgagaagg tcctctgtgc gcaccacttc
     7141 tgttccgtca tgataccggg tatatccttc ctgctgaagc atcaggaggt tctctaccct
     7201 gcgctgtgca tcctcccatc caaacggggc cagaatcatg gcagacgttc caggtggcag
     7261 ggatactatg tattgcagga catcttcctg ggtgtggcac ctgacttctt cccctgtaac
     7321 cggagaatat gtcctcccga tacgggcata taaaagacgc aggtaatcat atatttccgt
     7381 ggttgttccc agcgtggagc ggggattgcg cgtattgacc ctctgttcga tggcgatggt
     7441 gggcggtatt ccctctatgg aatccacatc gggttttttg atcctcccca ggaactgacg
     7501 ggcatacgca gacaggcttt ccacgtagcg cctttgcccc tcggcaaaaa gcgtgtcaaa
     7561 ggccagcgat gattttcccg aaccagaaac cccggtgatg actatcagtt tgtccctggg
     7621 gatatccagc gatatatcct tcaaattgtg aacgcgtgcg cccttgacga caattttttc
     7681 ttcatccatg accttactcc cttaccgctt tagtgatccc ccttatcttc ctgagctgga
     7741 acaacagcaa atcaacctgt ttgttggagc tgaccgccac ctgcaacttt cctgcaattt
     7801 ttcctccctg gcccgagaac gtgaaactgc gcagattgag cgaaaggttc cctatcacat
     7861 cgctgatctc ctgtgccagc ccgatttcct ggtacgccgt gaacctgatg gtaatctgga
     7921 acatagccga ggtgaccttt tcgcgccacc tcactttctg tatccggtag ggataccggg
     7981 tcatcagcct ggcggcattg ggacagctca tcctgtgtat cttgatacct tccttgatgg
     8041 acacgaaccc gaacacttca tcccctatca ccgggttgca gcacctggcc agtttatagc
     8101 caatcctgcc aaaattatca tctattacca gatagtcacc ctccctgtag ctggtttttt
     8161 cttcagcgtg ttccggcggc gtgcttcctg tctccggttt ctcagatgta tcttctttat
     8221 ccttcagcaa aaacaacttg atatcctgga gggaaatatc ttcccgggca acagccgcat
     8281 aaaaatcgct gatctgcttt atccggaagt gtttcatcaa ctgcatcaaa tcctccggtg
     8341 tcagttccat tttccagttt ttcaaccggc gttccaacat ctccctgccc gccacatcct
     8401 ggattccttc ttcctcacgc agtttctggc ggatcttgtt cctggccttt cccgatacca
     8461 caaagccgag ccagtccctt gacggctttt ggttcctgga cgtgaggatt tccaccacat
     8521 ctccggtttg cagggtatca cggacctgtg acatctttcc attcaccctt cctccggtac
     8581 atctaaggcc tatgttcgag tggatctcaa aggcgaaatc aagcaccgtg gccccggcgg
     8641 gcaggcgtct taaatccccg gccggcgtga agacgtagat ctcgttggac ttgtacagat
     8701 ccttttcagc agaagaaaaa tcttcctgcg gattctgcag cctcttcctg accccggcaa
     8761 gccagtcatt catcacctgt tcgtttttca cgcctttgta agcccagtgc gctgccacgc
     8821 caaactcggc tacaatatcc atcctgcggc tgcggatctg cacctcaagt gcagctcctt
     8881 ccggcgtacg cacggtgata tgcagcgatt catacccgct ggccttgggt accgtgatcc
     8941 agtcccgcat cctctcggga atgggttcat aaatctgcgt gactattgaa taaacctgcc
     9001 agcagacctc cttttccctg tcaggtgtag tgtccacaat aaaccggatg gccaggatat
     9061 cgtatacctc atccacgcct acctgctgta tctgcatctt gcgccagata gaataggtgg
     9121 tttttgtacg gctcttcagc tcataacgga ttccggcttt cctgagttct ttttccaatg
     9181 gttgcacaaa cccggcgatc tgcttctttc gctctatttc attagccctc aggttattgg
     9241 tgatcaaacg gtaatcttca ggctggatta ccttgaaaga aaggtcttcc agctcgctct
     9301 tgatcctgta caaacccagc tggtgtgcaa gcggggcgta cagcatcatg gtttctgtgg
     9361 cttttttgat atggtcgttt cggggaaaac aatccgccag ggaccgcatt acttccagcc
     9421 tgtcggcaag tttgatcagg gtaacccttg gatcggcaga ataggaaatg atcagtttcc
     9481 tgtaattctc ggcctgcagg cgcgtatccc tgggattgat ggtagatatc ttgttcagcc
     9541 ccacgaccat ggagccggtt tcatggccaa ataaagaagc gatctctgcc agcagttccg
     9601 gatgtttacg gctggcctca tgcaggtaaa ccgtagcaac tgcctcgccg gcaaggccga
     9661 tttccttttc cacgatggcc gccaccgcgt ccacgtgctg catgaaggga gcgccattgc
     9721 cccgtttcag cccttccagc ctggcggcag catactcctt tgccttatcg atcatggttt
     9781 gattcatttg cgtaattttt caagaccata catttcatca cggaacctgg cagccgtaat
     9841 gaaatccagt ttggtggccg cctcttccat tcttttcctg gccgtcgcta tggctttttc
     9901 cagttgttgc ggagtcatgg cccgggctac cgggtcttca gccaccatgg aaatgacatc
     9961 cggtgcctcg ctgtaggccg gttcctggct catggtacga cggatattaa gggctatctg
    10021 ggtaggagtg atcccgtttt cacggttgta ttgcatctgc ttgttccggc gtctatccgt
    10081 ttcctcaatg gtccgggcca tggaatcggt tatcttgtcc gcgtacatga tcacgcgccc
    10141 gtccacgttt cgtgcagccc ttccggccgt ttgggtaagg gctcgctccg aacgcagaaa
    10201 gccctcctta tcggcatcca gtatggccac cagggaaacc tgcggcaggt ccagtccctc
    10261 acgcaacagg ttaaccccca ccaggacatc gtaataccct ttctggaatt cctccagtat
    10321 cctgatgcgc tccagcgtat ccacgtcaga gtgtatatag gtgcaacgaa tgcgtatctt
    10381 ctgaaggtat ttgtccaatt cctccgccat cctttttgtc agcgtggtga ccagcacacg
    10441 ctggtctttg gcggcccgtg acctgatttc ttcaatcagg tcatctatct gcccttccgt
    10501 cttccgcact tcaacaggcg gatccagcag tcccgtgggc cggaccacct gttccactat
    10561 cagtccacac gagcgctcca tttcataatc tgccggtgtg gccgaaacat agaaacgctg
    10621 cccaatgacc ttgtcaaatt ccgcaaaggt caggggccgg ttgtctgcag ctgccggcag
    10681 gcggaatccg tactcaatga gcacttcctt gcgggaacgg tcacctccga acatggcccg
    10741 gatctgggga agtgtcacat ggctctcgtc aattatagtc aggaagtcat ccggaaaata
    10801 atccagcagg cagaaaggcc ttgtccctgg aggtcttccg tcaaaatacc gggaataatt
    10861 ctcaattcca gggcaataac ccagctcacg tatcatttct atatcgaatt ccacgcgttc
    10921 caacaacctc cttgcttcca gatgtttgcc caattctttc agtgtggcca ccctctccgt
    10981 caggtcgtcc tgaatgctcc ttatggcctg ttcctttctc tctttggtgg ttacaaaagc
    11041 gctggccgga taaatggcga tgccttcaat cctttctatc accccccctg tcatgggatc
    11101 cacgcgttcg atggagtcaa tgcggtcgcc ccagaaggag atcctgcagg caatatcatc
    11161 ataggccagg taaatgtcca caacgtcccc gcggaccctg aaagtccctc ttttgaactc
    11221 gttttcgttg cgcgaataaa gcgcatccac cagcctgtac agcaatttgt tgaaatcgtg
    11281 taactccccg gttttcaatg tgatggtatt ggcgtggaaa tcttccggat tccctatgcc
    11341 gtacaggcag gaaacacttg ataccacaag cacgtcacgc cggccgctta acaaagagga
    11401 agacgtgctc aggcgcagtt tctcaatctc gtcgttaatg ctcaggtctt tttctatata
    11461 gacatcagtg acaggcaaat atgcttcagg ctggtaataa tcatagtagg aaacgaaata
    11521 ttccacggca ttctccggga aaaaactttt gaattcggcg tagagctggg cagccagcgt
    11581 cttgttgtga ctgatgacca gtgtgggtct ttgcaaccgc tccaccacat tggccatggt
    11641 gaatgtcttt cctgaacccg tcactccaag cagcacggcg gcctctgtcc cttctgccag
    11701 gagagaggct aactgttcaa tggcctgcgg ctgatctccc gtaggtgcat aaggtgccgt
    11761 gagcttaaat tgcataggaa tgattcaaga cagcaaattt actgaaaaac gtcagtacat
    11821 caaatacccc gccaagccac ccagggccag cagcaaaata ggatgggccc tgtagaagta
    11881 tgcagccagg aaactggctg aaaagagcag gtagctgcgg aaatccacaa aattttctgg
    11941 tgtggccatg ttcagtacgg cagcagcgat caatccggcc acaaccggac ggatgccact
    12001 gaaaatggcc tggaaagtct tgttttgact gtattttctg tacaccctta tcacaacgga
    12061 catcatgacc acagaaggga tgatgagcgc aatggtggcc atgaaagaac ccaggacatt
    12121 tcctcccgag gctgtataac ctacatacgt agcggtattt atggctacag ggccaggcgt
    12181 catctgcgat acagccacaa tatcggaaaa gctctccatg gtcatccaac cgttattgcg
    12241 gaccacttcg tcctggatga tcgataacat ggcatagcct cccccaaaac aaaaaacacc
    12301 tatcttgaag aaagtgtaga ataattccag gtatatcatg tgcttcgcct cctcttgcgg
    12361 tgtttatcaa tggtgtacca ggcataccct cccagacctg cagccaggat gatccagacg
    12421 ggagatacgc ccaacagcca gatcagcagt gttgcaacac aggctatcag cccggttgca
    12481 taagtcagcc cgatggaacg catggtggtg atgacaggaa cagctatcag ggccaccact
    12541 acgggacgca tgcccaaaaa gatcttctcc acgacgggat tatccctgaa attctgaaag
    12601 aagatggcca gggccaggat ggaaaaaaac ggggcacaga cagcacccag caccgatacc
    12661 agagaaccgc gtgttccttt gactttatag ccgaccgcca ctgccatatt cacggcaatg
    12721 acaccgggca gtgtctgggt tacagccaca atatccgtaa aaactgtttt tgttatccac
    12781 tttttacggt ccaccacctc cctctcgatc accggcaaca tggcaagccc tcctccaatg
    12841 gtgaaggccc ctatcctgaa gaacaccttg aaaagttccc aatacatggt taatccaata
    12901 tgatttccac aaccgtattg atcagagacc ggtcataagg gcgttctatc cgtatgtagt
    12961 tgcgggtata cccgtgcatt ttatcattcc ctgtctttcc ctcaaacaaa acttcctctt
    13021 tccttccctt gtttgcgagg ataaactcct ggtgcagctt gtcggacaat tgacccaaca
    13081 tacgcgtcct ttcggttatg atgccttcgc ggatcctttc cgacatcggc aacttggcgg
    13141 caggtgtgcc gggccttgga gaaaaaggaa atacatgcag gaaggaaggt ttcagtgttg
    13201 ccagcaggtt atgggtttcc ataaaatcag cctccgtttc accaggaaaa cctgcgatca
    13261 catcaacgcc tatgaatgca aaaggcatca gggaacggat atgacttacc ttgccggcaa
    13321 acagctccgg gtcatacctt ctgcccattt tcctgaggat ccggctgctg ccgctttgca
    13381 ggggtatatg gaaatgagga aggaaacact tcagccgggc cgtcatttct atgatctccc
    13441 cggtcaggag atttggttca atggaagaaa tcctgtagcg ttcaatccct tctacatatg
    13501 acaactgtct gagaaggtca gcaaaagact cccctgttgt ctttccgaaa tctcccgtgt
    13561 tgactcctgt caagacaatc tccttcaccc ccctgcccgc gatctccctg gcctgcagga
    13621 ccacttcggc tatgggaatg ttcctgcttc ttcccctggc cagcggcacg gtgcaatagg
    13681 tacaacaata atcacaccca tcctgtactt tcagaaaaga cctggttctt tcacgatggt
    13741 cctcacctcc ggaccaggcg ggaaaaaaaa cacttcccac agtgcccgtg ccggggaata
    13801 gggccccaac gcccggatta ccagccactt ctcctacccg gtcacggaaa tccgtaccca
    13861 gtacagtctc cactccagga atcagacgca ggctttctgc cctcagctgt gcagagcatc
    13921 ctgtaacaat gatttgtgtt tttggattga tatgcaaggc tttacgaatt gcttgcctgc
    13981 tttttttatc tgcgtgctcg gtaactgaac atgtattgat gatggtaata tcggcacccc
    14041 tggaaagccc taccctctga tagcccctat ggagcagatc cctggccatg gtagcacttt
    14101 ctgcataatt gagcttgcag cccagtgtta ttacagtaaa tgtacacgcc tcattcatgc
    14161 tgcaaatcct tcatggtcag ggtaacagaa gcgctgccca cttctccccc gcccaacgcg
    14221 ggtgatagtt ttgaaacttt cactcgacaa tcggacaatg aaaaagaccg gcatagtgag
    14281 cgaaggatcc ttccggcaac gtgttccagc agccggctgg gtatggacat ttctgcactg
    14341 accagttcgt atacacggga ataatcaacc gcgtcctcca gcttgtccgt aaaggcagcc
    14401 ggacagccgt ggattgacaa ctccacatct accgaaaaaa aagtaccaat aagacgttct
    14461 tcgtcaaagc acccgtgatg ggagtaaaat ctcagatctt tcaaagcgat ggttgccata
    14521 tatcaagtct gtaataaaaa tacacacggt tttttcctgt atgcagctga gaaatcaggt
    14581 tctttgccct tccactcccc tactgtcctg gtgcatatga atgcatcggg cagagtaagg
    14641 ttaatggcca ctgcaagccg ggtcccggga gcacagcaat ccagcagggc ccggaaaagc
    14701 gactcattcc tgtaaggagt ctcaataaaa atctgggtct ggttttcctg ggccgattgt
    14761 ctttccagcg cgcggatcct tttatcccgc aaaccggatt ttaccgggag gtatccctgg
    14821 aatgcaaatt tttgtccatt gagccccgag gccatcaaag ccagcatcag ggaagagggg
    14881 ccaaccagtg gtatcacctg gaagccaccc cggtgagcca aagccaccag ctctgcaccg
    14941 ggatctgcca cagccggaag gcctgcctcg gacaacaaac ccacggaacg cccctttctc
    15001 aagggttcca gcaagccatt cagttctgcc ggagacgtat gttcgttcag cacctcaagt
    15061 tcaagagacc gcacatcaag acccagtgcc gagagatacc gtctggccgt tctgggctct
    15121 tccacaatga aacaatccag ttttctaacc atttccacgt ggtctgcagg aagtaccccc
    15181 gatggagtcg tgtctcccag gggagcgggt accaggtaca gggccggttt attcattgtg
    15241 ccttcacctc cttgttcagc ctttctgtca gggccctttg gaatctcctg gcatttttca
    15301 tatgatcacg cagattcagg gcaaaattgt ggtaaccgct taaatcttcc tttgcacaga
    15361 agtacaaata atcatggtgg tcatagttca gcactgcatc aatggcctca atggaaggca
    15421 ctgctatggg acccgggggc aaacccttgt acttatatgt gttatacggt gattcaatgc
    15481 gcttgtgcct tgccagcacc ctccgtatgg aataatcctg tgcagcaaat atcagggtgg
    15541 gatctgcctg caaaggcatc cctatcctga gacggttcat gtatacgcct gccaccacag
    15601 gcatttcatc tgttttcttg gtctcttcgt tgacaatgga tgccagtgtg ctcacctgcc
    15661 tgatggtcaa accaatgctc ctggcttttt ccttcctttg ctcattccag aaactgttgt
    15721 attcacggga catcctctcc agaaggtctt ctccgcttat ggtccagtag acctggtagg
    15781 tgtttggtat gaacatgcct ctcaggctca ccgaatcaaa gccaaacgtg gctataaggg
    15841 aatcgttcct caaatgttgg agcaccaggg ctgaatctat ttcaaggttg cgggccagga
    15901 ccgaggcgag cctttcaaaa gtcctgatag gggcatttat agttatgttc aggggatcct
    15961 gccaccccat ggccagtttc ctggccagtt cccggttatt cattccatgg gtcagggcat
    16021 agcgtcccgc atgaacattt tcaggcaggt tttcgtgttt ggcagcacag cggaaagtct
    16081 gttccatggt caggtgtccc cctgatctca ggctgtctat caattgctca taagtggtac
    16141 cccgaggtac cagcaaataa gcagtcttgc cgtcttccgt tgttatattg gggtagtgga
    16201 aagtcaggta ataccaccct ccggccacaa tgcctgcgcc taaggtcagg atgatgaaaa
    16261 gcaggaagaa tcgggacttt ttcctcatga tgttcttttt atcttaaacg gataacagtt
    16321 ccggccaggg gataatcccc tgttcccggg ttcaatgtac gtaatgactg caaccgtatt
    16381 ccgaaacgtt gtgatatttc ctgcagggat tcccctgaaa gggaagacca ggagcattct
    16441 cctcgggtca ccctgccggc cttgcgttcc aggtatactt tttctccaac tgccagttca
    16501 gcgtttgcag caacgtcatt atagcgtgtg agttcactcc ggaaaaaccc gaattcggca
    16561 gccagggacc ggtacgtatc tccgggggca gataacacgt aacgttttcc gttggtctga
    16621 agaattcttc tttccacatc aattacgaaa acatcttctc ctgtcattgc ataggagggc
    16681 atgccggcag cagggattcc cgacttcatt gcaaggggct cacgcccttc ctcttcctgc
    16741 aagggaagca aatcatattg atgtaaactg tacttctcaa tcaattcaac aagcagttca
    16801 tcatagcggg gattggtcgc ataccccgat tgtttcaggc ctcgggccca ggaggcataa
    16861 tctgttctct ccaacaggaa caggctggca taccgcggcc tggtcctgag aaactctgaa
    16921 tggtcatagt aggaatcttc tgcagtcctg tatttgcgga aacactcctg cagggcatca
    16981 tcatcgtggt aaatacggtc tccttcccag tcgtggcatt tgatcccgaa atgattgtta
    17041 ccttcccgtg caaggcggct gttcccgttg ccggattcca ggcatccctg tgcaaggatg
    17101 atgctggccg gaattccgta atgatacatc tgtgatatgg caatattctt gtatctattg
    17161 atatattctt cccgtgtgat ctgcctttcc gggtcttctg accagggaga ggccttagcg
    17221 ggaaccaata ccgggagtat acatgaaaca aagcagaatg ccttatatag ccacggattc
    17281 aacatgatgc aaatgtagta taaattctta tatttgcttt ctataaaccg cttacctatg
    17341 aacagaagta tccttaccat cgattataca gaaactacta aagacctcct ggaccccctg
    17401 gatgccgaac tcctggataa ggccgcccgt gctgctgacg gcgcctatgc cccttattca
    17461 agattcctgg tcggggcagc gctccgcctg gagaacggtc aggtcatatg cgggaacaac
    17521 caggagaacg cgtcctttcc ggcagggata tgtgcagagc gaactgcggt attttatgca
    17581 catgccaatt tcccggaaac acagatccgg gccatagcaa taactgcctc gccttgcggt
    17641 atatgcagac aggtcctcct ggaaagtgaa aaaagagccg gagtgcccat acgcgtattg
    17701 ctggcagccg gtgacaaggt tcgtattttt cagagtgtaa aagacctctt gccgttcggt
    17761 tttgattctt tctgattttt tgtacctttg cacggggtaa gtcgtgtacg accagctccc
    17821 actgaactcc cccagggttg gaagacagca agggtaggtg gttgtagcgg tgcgatacac
    17881 caagcttacc ctttttcaat tatga
//
LOCUS       DAOSOF010000025        17765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3210_length_17765_cov_5.534161, whole genome shotgun sequence.
ACCESSION   DAOSOF010000025 DAOSOF010000000
VERSION     DAOSOF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_17765_cov_5.534161"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLM68_02635"
     CDS             complement(<1..808)
                     /locus_tag="PLM68_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT54295.1"
                     /translation="MPEQIKTLALDKEHIPRLLFRYATPAIISMTAASLFNLADVIFI
                     GRGVNAMAIAGLSLTLPLMNISAAFGSLIGVGASTLISVKLGQKDMKTAEIALGNAVT
                     LNCIIGIIYTIAALVFLDPLLHFFGASEQTLPYAREYMQIILAGNVITHVYYGLNDNL
                     RASGYPKKAMFATLSAVAVNIILDPLFIFGFKWGIRGAAVATVLAQVLALVILLNHYT
                     NKQSLLRFKQGNFRIRKGMPLNIISVGLAPFLMNIVSSLVIIIINRSFYQF"
     gene            complement(801..2156)
                     /locus_tag="PLM68_02640"
     CDS             complement(801..2156)
                     /locus_tag="PLM68_02640"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOT54296.1"
                     /translation="MKRFVTLDVLRGLSIFGMVFSAIIPYGKLPGWMYHIQTPPPDHV
                     LDTASYGLGWVDLVFPIFIFCMGVAIPFAFRAKWKRSGQDVLRDMKDIGERFLMLLAF
                     SYLCDLLQPSLPGYWSEFLILAGFAALWLIYVKGASFKFRIAGWAAALGLLALYAFVF
                     DVQMTLFSRNIIILLLAFLYLFGALIWYVTRDSEKWRLAAFLGVISISVVSRLLGFDY
                     ILYDNKNLSWLLNMEQINFLMILLPATWVGDRLLHMQEEQTTGIPRGGFLTHLLFWGM
                     FLLVIWICISSYNRWLSDGKWFSTFALLLLVWLMKKKAPQYLPLLYLSGLLIFFGLWI
                     ERFEPGLTKVPCSFSYCFVSGGIAILLLMWLHYLSEFFPRRFFSGIFSGAGANPLMSY
                     VAHGMLVAPLMRITYLEVLYRWARPADYPWIGTLSAFIVVLFTMWLVSLATQKKIYWR
                     A"
     gene            complement(2163..3494)
                     /locus_tag="PLM68_02645"
     CDS             complement(2163..3494)
                     /locus_tag="PLM68_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOT54297.1"
                     /translation="MGVFHAYDIRGIYGKDLLPEDIYKMGLFLPSVLKTKRIAVGRDC
                     RESSPEAFKYLVRGLCDAGANVFDLGLATTPMVYYATSKHHFDGSVQITASHNGAEYN
                     GLKVSGREAMPIGYDNGLKELEDCVLHKVPVITEKRGTVTQLDLRDEYFSYLSAFVPH
                     LKGLRVGIDVSNGMAGLFVKKLLGTENVFYINEEPDGRFPNHAPNPLVPENIKQLQEL
                     VRNRQCDLGIIFDGDADRVMFTDEKGMFISPDLIIALLGHHFLDGKPARVLQDIRSSK
                     AVGEYLSPMGAHMHTWRVGRAFAAPKLKEIDGLYGGELAGHYYFRDFFYSDSGLLASL
                     LVLDVLVKLGKPVSEIIGGIRKYESSGEINFRIARKKEAMEALKAHFTAQEDPTAFFD
                     FDGYRIEFAHWWFNVRPSNTEPYLRFIAEAANGELLSEKLGEMRMVLKPYL"
     gene            complement(3496..4050)
                     /gene="def"
                     /locus_tag="PLM68_02650"
     CDS             complement(3496..4050)
                     /gene="def"
                     /locus_tag="PLM68_02650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT54298.1"
                     /translation="MILPVYIYGSSVLREKAQDITPDYPNLDKLIGDMWETLYHADGV
                     GLAAPQIGLSIRLMMVDGSELSEDRPELKDFKRLMINPSILEVSEQTVIHQEGCLSVP
                     KLWADIERPESVKLAYLDQDFNPVEEWFHGMAARMVQHEHSHLEGVLFTDLAPAIRKK
                     MLQSKLQAIARGEVKTFYKTKIVK"
     gene            complement(4047..4463)
                     /gene="ruvX"
                     /locus_tag="PLM68_02655"
     CDS             complement(4047..4463)
                     /gene="ruvX"
                     /locus_tag="PLM68_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT54299.1"
                     /translation="MGRIMGIDYGQKRVGLAVSDPLNIFATPLETVTVDQVTAFLKTY
                     IEDEPVDLFVVGYPRQPDNTPAQNASRVTAFVTHLKRTFPRIPVTLVDERFTSKMAFR
                     AMIDGGLKKKARKDKATVDKVSAVIILQEYLQSNKL"
     gene            4523..5383
                     /locus_tag="PLM68_02660"
     CDS             4523..5383
                     /locus_tag="PLM68_02660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT54300.1"
                     /translation="MAPGNSKKKIVRDRYHFGIFDLESHKPILNFKFSILKLLLWLLG
                     IITVLITSTVLLMIYTPLKEIVPGYPSARTREVMMYNALRADSLEKVIQLWEIHLTNL
                     QRVLAQEEPMAPGQILPKGQTTDIPEFAVGGRSQEDSLLRAGAALKEQQSRSSISGGS
                     LQIEGLHFFPPVKGLITSTFNPAENHFAIDVVAPENSVIYAVLDGTVNFASWTDEFGY
                     VLQIQHENNLLSIYKHCSQLFKKVGDPVTAGSAVAVIGQQGTFSTGFHLHFELWHKGV
                     PLNPADYINF"
     gene            5385..6548
                     /gene="dxr"
                     /locus_tag="PLM68_02665"
     CDS             5385..6548
                     /gene="dxr"
                     /locus_tag="PLM68_02665"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT54301.1"
                     /translation="MAFQKRIAVLGSTGSIGIQTLDIIRKYPGFFKAQILTARSSSDL
                     LIRQALEFQPEAVVVTSRDAWQVVRDALEGTPVQVLYGNDSIAEVLAWDTVDLVLNAL
                     VGFIGLEPSLAALSNHKILALANKESLVAGGHLVLREATAGKGSILPVDSEHSAVFQC
                     LRGEQATIEKIILTASGGPFFRHSARELESITKAEALDHPTWNMGAKVTVDSATLMNK
                     GLEVIEAYWLFGQPLSRIEVMIHPQSLIHSMVQFTDGTIKAQMGYPDMHLPILYALSF
                     PRRMDYPDAPRMELAHFRSMEFHMPPADRFPCLEMAYQAMKTGGNTPCVLNAANEVAV
                     DAFLHERLNFTAIPKLISQTLEKVSYIADPTFSILKETHEEAQRVAQILLASY"
     gene            6553..7881
                     /gene="rseP"
                     /locus_tag="PLM68_02670"
     CDS             6553..7881
                     /gene="rseP"
                     /locus_tag="PLM68_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT54302.1"
                     /translation="MEVLIKIAQVILALSFLILIHESGHFLFARLFKIRVDKFYLFFD
                     PWFSLIKFKPKNSHTEYGIGWVPLGGYCKISGMIDESMDKEQIREEPKPWEFRSKPAL
                     QRFLVLFGGVFFNMILAVILYGLILYTWGEEYIRTEDASYGVHCSPLAQEIGFRNGDR
                     IYAFDDRVVIDEPFHTLQIDLIRTRPAHITVLRGEDTVRFGFDQSYIAQMLNTQNIFT
                     LRYPFVISSVPDTSLNVSTGLLPGDHVVSLNGIPTPAIQDVQEILAQNSEGVVEATIQ
                     RDLSVLQIPLKVNSKGMLMVGVVSPLEFLPVTIKEYSFFGAMPAGIKKAYQTGANYLR
                     ELGLIFTPKTKAYKSVGSFIAIGSIFPGTWNWEIFWNITAWLSIMLAILNLLPIPALD
                     GGHLVMVIYEMITRRKPSDKFLEYSQVVGMILLLALVFLAFGNDLFRLFT"
     gene            7900..8886
                     /locus_tag="PLM68_02675"
     CDS             7900..8886
                     /locus_tag="PLM68_02675"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOT54303.1"
                     /translation="MRTKTLLIAGFIVILATSCTDDANLKQNISGKAGEIMVVMNKYS
                     WESGPGQSLRSILAVDFPYLPQQEPLFTIFNINENAFSSIFHTHRNIIICNINSELDE
                     AKMVIQKDIWASPQIVVTLSGPDNESVRECIDNNRDLLRNALEQAERNRVIQNSKKFE
                     ERSIRNYVSTMLGGSPFFPTGYKIKKRADNFTWIAYETTYTTQGVFIYTYPYRNEEDL
                     TLSRIIAERNLKLEKEVPGPLDNTYMTTNSLIEPSLRWVNYNNRQFVEIRGLWDVKND
                     FMGGPFVSHCFYDKDNHNIVVLEAFVYAPRYPKRDYLRQVESIIYSFEWQKE"
     gene            8935..9009
                     /locus_tag="PLM68_02680"
     tRNA            8935..9009
                     /locus_tag="PLM68_02680"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8968..8970,aa:Glu,seq:ttc)
     gene            9027..9281
                     /gene="rpsT"
                     /locus_tag="PLM68_02685"
     CDS             9027..9281
                     /gene="rpsT"
                     /locus_tag="PLM68_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT54304.1"
                     /translation="MAHHASAEKRNRQNITRRLHNRYYARTTRNAIKAVRNTTDKATA
                     VAMLPEVFSMIDKLAKINVIHDNKASNLKSGLNAYINKLA"
     gene            9311..10036
                     /gene="radC"
                     /locus_tag="PLM68_02690"
     CDS             9311..10036
                     /gene="radC"
                     /locus_tag="PLM68_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT54305.1"
                     /translation="MLPNFWEAFSFFIMDRHLKITDWAKEERPRERLLANGPSSLTNA
                     ELLAILLRTGTGKANVMDLARNLLAAHENQLYELVRAPVEKMTKVRGIGITKAATLCA
                     AFELGRRAQLPVERPNAITSAGEVAQMMIPMLRDLTHETCWVLFFDGGRKLIGKEQIS
                     SGGLNATIVDVRMILKKALDKLSCELILVHNHPSGSNKPGRQDRILTQTLKEAARMVD
                     LHLIDHIVIGGNGYYSFAEEGLL"
     gene            10078..10731
                     /gene="upp"
                     /locus_tag="PLM68_02695"
     CDS             10078..10731
                     /gene="upp"
                     /locus_tag="PLM68_02695"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOT54306.1"
                     /translation="MTTYVFAEKDSCFNPVLAQMRDINIQKDSMRFRYNLERLGYLFA
                     YEISKTLSYREEEVQTPLGISRCKVLNDQIVLGTVLRAGLPIHEGMLKIFDNAQNAFI
                     AAYRKYGRNYKFTIQLEYLTSPSLEGKVLILSDPMLATGSTILLTYQELVQQKGTPKH
                     CHIVSPIASREGYEYLCRKLPQKGVTIWLGAIDEEMTTKSFIVPGLGDAGDLAYGDK"
     gene            complement(10733..12373)
                     /locus_tag="PLM68_02700"
     CDS             complement(10733..12373)
                     /locus_tag="PLM68_02700"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT54307.1"
                     /translation="MHKQTIALIVTGMTLILSTSCTTRRHVDLVVYNARVYTVDSSYS
                     ACEAFAVDDGIFIASGTTGQIREQFKGRRELDLNGAPVYPAFNDAHSHLFQVAQGLRY
                     VDLRGAASVDEVIARLQKHYDLHHPGFLVGDGWDQTLWENKDLPDNEKLNKAFPNIPV
                     YLRRVDFHATWVNEVAIALAGLHAGDISIPQGEALMNTSGQFTGVFLENTSDRINATI
                     PHMSDQELVACILEAQDLCLAFGLGSVTDAGLPLGRIKLLDSLQKAGALKLRIDVWMD
                     PSEENFSYFTRPYQSDRLDISAVKLYIDGALGSSGALLYEPYSDDPLNKGIQFTSDRN
                     FLAACEKAYERGFQVATHAIGDKGVGKVLDLYGQFLEPGNDLRWRIEHAQVVAPDDFK
                     RFGELNIVPSVQSTHATADMGWAVARLGPERIKGAYAFRELICAAGWAPFGTDAPVES
                     INPLYTFFAAVSRMDRNFNPPGGWQTENAVSRENTLRGMTIWAARGSFRENDKGSIEP
                     GKWADFIVWSGDLMTLPVEEIPGTSCCMTFIGGEQVYP"
     gene            complement(12375..13787)
                     /gene="rpoN"
                     /locus_tag="PLM68_02705"
     CDS             complement(12375..13787)
                     /gene="rpoN"
                     /locus_tag="PLM68_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOT54308.1"
                     /translation="MLSQSLQQKLQLKQKLSPLQIQTIKLLELPALELDQRVRKELEE
                     NPVLETKEGDEEPESAGELSLSEYTGQDDSIPSYKLYVNNQSRDMKKEFPTFSVRESF
                     HQNLEAQLGFRSMTDKEREIALFVIGSLDDDGYLRRDFETLSDDMAFRLGLEAGPREI
                     EQALSIIQEFDPPGVGARDLQECLLLQLRNKEQKPAVETAHTILEKHFAEFTKKHYDK
                     IIARLGITEDELKEAIEEILRLNPKPGGNVEDSYTDRAQQVVPDFMLEIKEGEPVLSL
                     PRFSVPELRINKRYADMLLKAAGQSTRESKEAATFVKQKLDSAKWFIEALKQRQNTLF
                     NTMKAILDFQKDYFAEGDETKLKPMVLKNIAEITGLDISTISRVVNSKYIQTHFGIYS
                     LKYFFSEGLMTESGEEVSTREIKNILVQSIEQEDKHKPLTDEELVACLNEKGYKVARR
                     TVAKYREQLNIPIARLRKQI"
     gene            13868..14857
                     /locus_tag="PLM68_02710"
     CDS             13868..14857
                     /locus_tag="PLM68_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54309.1"
                     /translation="MEPIIPPVSIHLLEEELTRERFVRYSSRGENKLFDVNAHEAPNT
                     MREIGRLREIAFRAAGGGTGYASDIDSYDVSPETPYRQLVVWDPKRKEILGGYRYIIG
                     AGIRPQNLATYPIFSFSDNFVNNYLPFTIELGRSFVQPNYQNSNENRSGLYALDNLWD
                     GLGALIVRYPHMKYFFGKVTMYRTYNKQARNCLLYFLNKYFPDPDSLATAIDPLDYNA
                     EKPYYRNIFVAGNYDDDYKTLVREIRTYGERIPPLINSYMKISPNMRVFGTTLNEHFG
                     GVEETGILITIKDMYPDRVGRHMNSLRHFAESVGLRWWNKDASKKAPEDQSGG"
     gene            complement(14842..15321)
                     /locus_tag="PLM68_02715"
     CDS             complement(14842..15321)
                     /locus_tag="PLM68_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT54310.1"
                     /translation="MNRFVLAAILVLLTGCYFSDAALAQVPDTLSQGTDVFEYFQRPG
                     PEGNRLRIIQSDSIRLKVNRQAEQNRFSQKKMQGFRVRIFFDNKQTARTESETIAMSF
                     MAMYPHTAVYRIYENPYFKVTVGDFRTRSEAMRFMMEVRKTYPQAFITREAIAYPPL"
     gene            complement(15318..15779)
                     /locus_tag="PLM68_02720"
     CDS             complement(15318..15779)
                     /locus_tag="PLM68_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54311.1"
                     /translation="MNRKSLILCILAIMMAVGCSRYRDIAIENIAVRQFNMTSLSTAR
                     VKISATLNNPTGGKIRLTGMNGIIRLKDKRIAQFSMDSALVFSARGTSTNEGLLSLRI
                     NDMSVLFSGAVDLDETLLRQIRLDIDATVKSGGIKRKLQLNDIPAKNLLDL"
     gene            15824..17251
                     /locus_tag="PLM68_02725"
     CDS             15824..17251
                     /locus_tag="PLM68_02725"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT54312.1"
                     /translation="MIPFYIETYGCQMNVSDSQVVASILSDNGYESCDSWEAACLVLI
                     NTCSIRENAEKRVMSRLDFFARVKRKNPQLKVAVLGCMATRLGDSLLIHPAVDCVAGP
                     DSYRLLPAMLDTPGSQPSPRPGMKHPVASSNVKYNILSEDGMPGGPLPARMNVELSAT
                     ETYADIRPLRNISSGVTTFVTIMRGCNNMCAYCVVPYVRGRERSRDPNSILEEVKNLV
                     DSGYREVTLLGQNVDSYLWQPKDGSEPVPFARLLEQVALVSPLLRVRFTTSHPKDMQD
                     DVLMAMARYPNICKHIHLPVQSGSNRILQKMNRKYTREGYLERIARIREILPGCAISS
                     DMIAGFCSETEEDHAMTLSLMETVHYDQAFMFQYSQRPGTLAARHYPDDVPEEVKTRR
                     LAEVIALQNRLSLQSNRADVGKIFEVLAEGVSKRSSRAFFGRTTTNKVCVFPRENRAI
                     GDYVRVLVKSCTAATLKGEIINENQ"
     gene            17255..17662
                     /locus_tag="PLM68_02730"
     CDS             17255..17662
                     /locus_tag="PLM68_02730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT54313.1"
                     /translation="MTKIKATYLGDLHVEAIHLQSGSRIETDAPVDNQGKGELFSPTD
                     MFATSLAACMMTIMGISARSYGFALEGAYAEVEKIMASSPRRVGEIIIDLYFPDGKTY
                     GDREKRIIEAAAATCPVANSVHPDLKKTVRFHY"
BASE COUNT         4681 a         4396 c         4248 g         4440 t
ORIGIN      
        1 caaactgata gaatgaacgg ttgatgatga tgattaccag gctgctgaca atattcataa
       61 gaaaaggggc aagccctaca gaaatgatat tgagcggcat gcctttccgt atgcggaagt
      121 tgccttgttt gaagcgaaga agggattgct tatttgtata atggttcaaa aggatcacaa
      181 gggcaaggac ctgggccagc accgtagcga cggctgctcc gcggatcccc catttaaaac
      241 cgaaaataaa gagggggtca aggataatat tgacggcaac tgcgctcagt gtggcaaaca
      301 tggctttttt cggatacccg ctggccctca ggttatcgtt caggccatag tacacatggg
      361 tgatcacatt cccggccagg atgatctgca tgtattcccg cgcgtagggc aaggtctgtt
      421 cgctggctcc gaaaaaatgc agcagaggat ccaggaaaac cagtgcagca atagtataga
      481 tgatcccaat gatacagttg agcgtaactg cattgcccaa ggcaatctcg gctgttttca
      541 tatccttctg accaagcttc acagagatca gggtagaggc acctaccccg ataagggatc
      601 cgaaagcggc tgaaatgttc attagcggta gtgtcagcga caggcccgca atggccatgg
      661 cgttgacacc ccggcctatg aatatgacgt ctgccaggtt gaacaggctg gcagctgtca
      721 tggaaatgat ggccggtgtc gcataacgga acaggagcct gggaatatgt tccttatcca
      781 gagccagtgt tttgatctgt tcaggcacgc cagtagatct ttttttgagt ggccagggaa
      841 accagccaca tggtgaagag taccacaata aacgcgctca gggtaccgat ccacgggtag
      901 tctgccggtc ttgcccagcg atacaatacc tctaaatacg tgattctcat aagaggggca
      961 acaagcatac cgtgagccac atagctcatg agaggattgg cccctgctcc tgaaaatatg
     1021 ccggaaaaga atctgcgggg aaaaaattcc gacaggtaat ggagccacat aagcaaaagg
     1081 atggcaatgc caccgctcac aaaacaatag gaaaaagagc agggaacttt tgtaagcccc
     1141 ggttcaaaac gttctatcca cagaccaaag aaaataagca aacccgacag atagagcagg
     1201 ggaaggtatt ggggtgcctt ttttttcatc agccatacga gtaacagcag ggcaaaagtt
     1261 gaaaaccact tgccatctga aagccaccgg ttatagctgc taatacatat ccatattacc
     1321 aaaaggaaca ttccccagaa taaaagatgg gtaagaaagc ctccccgggg gattcccgtt
     1381 gtttgttctt cctgcatgtg aagcagccgg tccccgaccc aggtggcagg aagcaggatc
     1441 atcaggaaat tgatctgctc catgttcagc agccatgaaa ggtttttgtt gtcataaagg
     1501 atatagtcaa atcccagcag acgggaaacg acactaattg aaataacacc caggaaagct
     1561 gccaggcgcc acttttctga atcacgggtg acataccata taagagctcc gaacaagtac
     1621 aaaaaggcca atagcaggat aatgatattc ctgctgaaaa gtgtcatttg cacatcaaac
     1681 acaaaagcat agagcgccag caggccaagg gctgcagccc atcctgcaat acggaattta
     1741 aatgacgcgc ctttcacata gatgagccac aaagccgcaa atccggcaag gatgaggaat
     1801 tctgaccagt atcccggtaa agagggttgc agcaggtcac ataaataaga aaaagccagc
     1861 agcatcagga accgctcgcc aatatctttc atgtcgcgga gcacatcctg ccccgatcgt
     1921 ttccattttg ctctgaaagc aaaaggaata gccactccca tacagaaaat gaaaatggga
     1981 aagacaagat ctacccaacc cagaccataa ctggcagtat ccaggacatg atccggtggc
     2041 ggtgtctgta tgtggtacat ccagccgggt aattttccat agggaatgat ggcagaaaag
     2101 accatcccga aaatagacaa tccccgcaaa acatccagtg tgacaaaccg tttcataagt
     2161 atttacagat aaggtttcag aaccattctc atttcgccga gtttctccga cagcagttcc
     2221 ccatttgcag cctctgctat aaaacgtagg taaggctctg tattggaggg tcttacatta
     2281 aaccaccagt gcgcaaattc aatacggtac ccgtcaaagt caaaaaatgc tgttgggtct
     2341 tcctgggcgg taaaatgcgc cttcagtgcc tccatggctt cctttttgcg ggcaatccgg
     2401 aaattgatct cccctgagct ctcatatttc cttataccgc ctatgatctc agacacaggt
     2461 ttacccagct tcaccagtac atccagcaca aggagcgaag caagcaaccc gctgtctgaa
     2521 taaaagaaat ccctgaaata gtaatgtcct gccaattccc caccatacag gccgtcaatc
     2581 tccttaagct tgggagcagc aaaagccctt cccacccgcc aggtatgcat gtgggccccc
     2641 ataggggaca ggtactctcc aacggctttt gaagagcgga tatcttgcag caccctggcc
     2701 ggtttgccat ccaggaaatg atgtcccagc agggctatga tgagatccgg agaaataaac
     2761 atccctttct catcggtgaa catgacccgg tcagcatctc cgtcaaatat gatacccagg
     2821 tcgcattgcc tgttcctgac cagttcctgc aactgcttta tgttttccgg gacaagcgga
     2881 ttgggggcat gattggggaa acgtccatcc ggctcttcat tgatataaaa cacattctct
     2941 gtcccaagga gtttttttac aaaaaggcct gccatcccgt ttgaaacatc aattcccacc
     3001 cgtaatcctt tcaggtgcgg aacaaaagcg gacaagtagg agaaatactc gtctctcagg
     3061 tccaactgtg tgactgttcc ccttttttct gttatcaccg gtactttatg aaggacacaa
     3121 tcttccagct ctttcagtcc gttatcatat cctatgggca ttgcttccct gcctgacacc
     3181 ttaagcccgt tgtattccgc tccgttgtgt gatgccgtga tctgaaccga tccgtcaaaa
     3241 tgatgtttgg acgtagcata atataccata ggggttgtgg ccagacccag gtcgaagacg
     3301 ttggctcctg catcacacaa cccacggacc aggtatttga aagcttcggg agaagattcc
     3361 ctgcaatcgc gtccaaccgc tattcttttg gttttcagga cagagggcag aaataacccc
     3421 attttgtata tatcttccgg cagcaggtct tttccataga taccccggat gtcatatgcg
     3481 tggaatactc ccatgttatt ttacaatttt cgttttgtaa aatgtcttga cttctcccct
     3541 tgcaatggcc tgcagtttac tttggagcat ttttttgcgg atggcaggtg ccaggtccgt
     3601 aaaaagcact ccttccagat gcgagtgttc gtgctggacc atccgggcag ccatcccgtg
     3661 aaaccattct tccaccgggt taaaatcctg gtccaggtaa gccagtttga cactctccgg
     3721 gcgttcaatg tctgcccaca gctttggcac gctcaggcat ccctcctggt gtattaccgt
     3781 ctgttcgctc acctcaagaa tgctgggatt gatcatcagg cgtttaaaat ccttcagctc
     3841 gggccggtct tccgataatt cgctcccgtc aaccatcatc aaccgtatgg ataggcctat
     3901 ctggggtgca gccaacccca caccgtcggc atgatagagg gtctcccaca tatccccgat
     3961 cagtttgtcc aggtttggat aatcgggggt aatgtcctgt gctttctctc ttaaaacgga
     4021 ggaaccgtaa atatatacgg gtaatatcat agtttattgc tttgaaggta ttcctgtaaa
     4081 attatcaccg cactcacctt atcaacagtt gctttatcct tgcgtgcttt ttttttcaac
     4141 cccccgtcga tcattgccct gaatgccatt tttgatgtga aacgctcatc taccaatgtt
     4201 acaggaatac gcgggaaggt tcttttcaaa tgagtaacaa aagcagtgac tcgtgaagca
     4261 ttttgagccg gagtgttatc cggttgcctg ggataaccca ccacaaacaa atcaaccggc
     4321 tcgtcctcaa tgtatgtttt gaggaaagcg gtcacttgat ccacggtaac ggtttccagg
     4381 ggggtggcaa aaatattgag cggatcactt actgcaagcc ccacacgttt ctgaccatag
     4441 tcaatgccca taatccgtcc catgacacaa aggtaacaca aattggaaaa tgattcttac
     4501 atttgcccga taaagctaat ctatggcacc gggtaattcc aaaaagaaaa tcgtcaggga
     4561 caggtaccat ttcggaattt ttgaccttga atcccacaaa ccgatcctca atttcaaatt
     4621 ttccatcctg aagttgctgc tttggctgct ggggattatc acggtgctga taaccagtac
     4681 agtattgctg atgatctata ctcctttgaa ggagattgtt cccgggtatc ccagtgcccg
     4741 cacgcgtgag gtcatgatgt acaatgccct ccgggccgac tccctggaaa aggtgattca
     4801 attatgggaa atccacctga ccaaccttca gcgtgttctt gcacaagagg aacccatggc
     4861 acccggtcag attctaccca aggggcaaac tactgatatc cctgaatttg cagtaggagg
     4921 gagatcccag gaagattccc tgctcagggc aggggcagct ttaaaagagc aacagagccg
     4981 gagcagcatc agcgggggaa gcctgcagat tgagggcttg catttctttc ccccggtcaa
     5041 aggactgatt acttcaacgt ttaatccggc agagaaccat tttgccatag atgttgtggc
     5101 acccgaaaat tcggtaatct atgctgtcct ggacgggacc gtgaattttg cctcctggac
     5161 agatgagttc gggtatgtgt tgcagataca gcacgaaaac aacttattat ccatatataa
     5221 acactgttcc cagttattca agaaagtagg ggacccagta acggccggat cggcagttgc
     5281 cgttatagga cagcaaggga cattttcaac aggattccac ctgcattttg aactctggca
     5341 taaaggcgtt ccacttaatc cggcagatta catcaatttt taaaatggca ttccaaaaac
     5401 gcatagctgt actgggttca acgggttcta tcggaattca gacgctggat atcattagaa
     5461 aatatcctgg ttttttcaag gcgcagatcc ttacggcacg gtcctcttcc gatttgctga
     5521 tacgacaggc cctggaattt cagcccgaag cagttgtggt aacctcccgg gatgcctggc
     5581 aggttgtcag ggatgcactt gaggggactc ctgtccaggt tttgtacggg aacgactcca
     5641 tagcggaggt gctggcatgg gatactgtag acctggtttt gaatgcgttg gtcggattca
     5701 ttgggcttga gccgtccctt gccgcactga gtaatcacaa gatattagcc ctggcaaaca
     5761 aagaaagcct tgttgccgga ggtcacctgg tcttgcgcga agctactgcc ggcaaaggat
     5821 ccattttacc cgtggatagt gaacattccg ctgttttcca gtgtttaagg ggagagcagg
     5881 ccaccattga aaaaatcatc ctgacggcat cgggagggcc ctttttcagg cacagtgccc
     5941 gggaattgga atccataacg aaagcagagg cactggatca tcccacctgg aatatggggg
     6001 caaaggtcac cgtggattct gctaccctga tgaacaaagg ccttgaagtg atagaggcat
     6061 actggctttt tggtcagccc ctttcacgca tagaagtgat gatacatccc cagtcgctga
     6121 ttcattccat ggttcagttc actgacggga ccataaaggc ccagatggga tacccagaca
     6181 tgcacttgcc gattttgtat gccttgtctt ttccgcgccg tatggattac cccgatgcac
     6241 ccaggatgga actggcgcat ttccgttcca tggaattcca tatgcctccg gcagacaggt
     6301 ttccttgcct ggaaatggct tatcaggcca tgaaaaccgg cggcaacacc ccctgtgtcc
     6361 tgaatgccgc aaacgaagtt gctgtggatg catttttaca tgaacgtctc aattttacag
     6421 ccataccaaa attgatctca caaacccttg aaaaagtatc ttacattgca gaccctactt
     6481 tttctatatt gaaagaaaca cacgaggaag ctcagagggt ggcacaaatt ttgttagcaa
     6541 gttattgatt atatggaagt attgataaaa attgcccagg tgatcctggc cctttctttt
     6601 ttgattctga tacatgaatc gggacacttt ctttttgccc ggcttttcaa gatcagggtt
     6661 gataaattct acctgttctt cgatccctgg ttcagcctga tcaaattcaa accaaagaat
     6721 tcccatacgg aatacgggat cgggtgggtg ccgttgggcg gttattgcaa gatatccgga
     6781 atgatcgatg agtccatgga taaagagcag atcagggagg aaccgaaacc ctgggaattc
     6841 aggagcaaac ctgccttgca acgctttctt gtattgtttg gaggggtgtt tttcaatatg
     6901 atcctggcag tgatactcta tggtttgatc ctctatacct ggggagaaga atatatccgg
     6961 acggaagatg cgtcctatgg cgtccattgc agccccctgg cacaggaaat cgggttccgc
     7021 aacggggaca ggatctacgc ttttgatgac cgcgtggtca ttgacgaacc ctttcatacc
     7081 ctgcagattg atctgatcag gacaagacct gcgcacataa cagtgctcag gggagaagat
     7141 accgtccgtt ttggtttcga ccagagttat attgcacaaa tgctgaatac gcaaaatatc
     7201 ttcaccttgc gttatccctt tgtgatttcc tctgttccgg acacctctct caatgtgtca
     7261 accggattgt tgcccggtga tcatgtcgtt tccctgaatg gtatcccgac tcctgccata
     7321 caggatgtcc aggaaattct ggcgcagaac agcgaaggag tagtcgaggc aacgatacag
     7381 agggacttgt ctgtcctgca aattccgctg aaggtgaaca gcaagggcat gctcatggtg
     7441 ggtgttgtca gtccccttga attcttacct gtcaccatca aggaatattc ttttttcgga
     7501 gcaatgccgg cagggataaa aaaagcatac caaaccggag caaattacct gcgtgagctg
     7561 gggctgatat tcactcccaa aaccaaggct tacaaatcgg taggcagttt catcgctatc
     7621 gggagtattt tcccgggaac gtggaactgg gagatcttct ggaacataac agcctggctg
     7681 tccatcatgc ttgccatcct gaatttgctt cccatacccg cattggacgg tggtcacctg
     7741 gtaatggtaa tatatgaaat gattacaaga cgaaaaccat ccgacaaatt cctggaatat
     7801 tcgcaggtcg tggggatgat tctgcttctt gcgctcgtat tcctggcttt cgggaatgac
     7861 ttattcagat tattcacata acaaatcaag gaaaaagata tgaggacgaa aactttacta
     7921 atagcaggtt tcattgttat tttggcaact tcctgtacag atgatgcgaa tctgaaacag
     7981 aatatcagcg ggaaagcggg agagatcatg gtcgtgatga ataaatattc ctgggaatcc
     8041 ggtccgggac agtccctccg ctccatactg gctgtggatt tcccttacct gccacaacaa
     8101 gaaccgcttt tcaccatttt caatattaac gaaaacgctt tttcctcgat tttccatacc
     8161 catagaaaca ttatcatttg caatatcaat tccgaacttg acgaagccaa aatggtcata
     8221 caaaaagata tctgggcttc tccgcagatt gtggtcacgc tctccggacc cgataatgag
     8281 agtgtcaggg aatgcattga caataaccgc gacctgctaa ggaatgccct ggagcaggct
     8341 gaaaggaaca gggtgataca aaattccaag aaatttgaag aaaggtccat ccgcaactat
     8401 gtatccacaa tgctgggggg atctcctttt ttcccaacag gatacaaaat caaaaaacgc
     8461 gcggacaatt ttacctggat agcttacgaa accacataca ccacgcaggg tgtctttatt
     8521 tatacctatc cctacaggaa cgaggaagac ctgacactgt cacgtatcat tgccgaacgc
     8581 aatctcaagc ttgaaaagga agtccccggc cccctggaca acacgtatat gacgaccaac
     8641 agtctgatag aaccttcttt aaggtgggtt aattacaaca accgccaatt tgttgaaata
     8701 cgaggtctgt gggatgtgaa aaacgatttc atgggcggac cttttgtgtc tcactgcttc
     8761 tacgacaagg acaatcataa tattgttgtg ctggaagctt tcgtatatgc cccaaggtat
     8821 cccaaacgcg attatttacg ccaggtggaa tccatcatct attcttttga atggcaaaaa
     8881 gaataaaata atttgtgaga aagaaaattc gttatacctt tgcgctccca aattggccag
     8941 ttcgtctaac ggttaggact caagattttc attcttgcaa taggggttcg attcccctac
     9001 tggctaccaa actataatta agaacaatgg cacatcacgc atcagcagaa aaacgcaatc
     9061 gtcagaacat tacaagacgt cttcacaaca ggtattacgc gcggaccaca cgcaacgcca
     9121 tcaaagcggt tcgtaacacc acagataagg caacagccgt tgccatgttg ccagaggtgt
     9181 tctctatgat tgataaactg gcaaagatca acgttatcca tgataacaag gcaagcaacc
     9241 tgaaaagcgg tctgaacgcc tatataaaca aactggcctg atttatcctt tttgtcctgg
     9301 tttttacagc atgcttccca atttttggga agcattttcg ttttttatca tggacaggca
     9361 tctgaaaatc accgattggg caaaagagga aagacccagg gaaagactcc ttgccaatgg
     9421 cccttcatca ctgaccaacg ccgagttgct ggcgatcctc ctgagaacgg gcacaggaaa
     9481 agcaaacgtc atggatctgg ccaggaacct tttagccgca cacgaaaacc agttgtatga
     9541 gctggtgcgg gcaccggttg aaaaaatgac taaagtcagg ggcatcggca ttaccaaagc
     9601 ggcaaccctg tgtgctgcgt ttgagcttgg gagaagggcc cagctgccgg ttgaaagacc
     9661 caacgccata acatcggccg gggaagtggc acaaatgatg attcccatgc tcagggacct
     9721 gacgcacgaa acatgctggg tactgttttt tgacggaggc agaaaactga ttggaaaaga
     9781 gcagataagc agcggcggac tgaatgccac catcgtggat gtacggatga tccttaaaaa
     9841 ggccctggac aagctttcct gtgaactcat actggtccat aaccacccct cgggcagcaa
     9901 taagcccggc agacaggaca ggatcctgac acaaacactg aaagaggcag ccaggatggt
     9961 tgacctgcat cttatagatc acattgtgat aggggggaac ggctactata gctttgccga
    10021 ggagggactc ttgtaattcc gcggactttt gtacctttgg agaaaatatc cccttttatg
    10081 acaacatatg tttttgctga aaaggattct tgtttcaatc cggttctggc gcagatgcga
    10141 gatatcaata tccaaaaaga ctccatgcgc tttcgctata acctggaacg cctgggttac
    10201 ctgtttgcgt atgaaatcag caagacactg tcatacaggg aagaagaggt acaaacccct
    10261 cttggcatct ccagatgcaa ggtgctgaac gatcagattg tcctgggtac ggtactgcgt
    10321 gcgggattgc ccatccacga gggcatgctc aaaatattcg acaatgcgca gaatgccttc
    10381 attgctgcat atcgcaaata cggcaggaac tacaagttta ccattcagct tgaatacctt
    10441 accagtcctt ccctggaggg caaggtgctc atcctttcgg atcccatgct ggccaccggt
    10501 tccaccatcc tgttaacata ccaggaactg gtacagcaaa aaggcacgcc caaacattgc
    10561 catattgtat cacccatcgc atcccgggaa ggatatgagt acctctgccg taaattgccg
    10621 caaaagggag ttaccatctg gctgggggcc attgatgagg aaatgacaac taaatccttt
    10681 attgtccccg gtcttggaga tgccggagac ctggcttacg gcgacaaatg aatcacgggt
    10741 agacctgctc tccccctata aaagtcatgc agcatgacgt gccggggatt tcttccacag
    10801 gaagtgtcat cagatcacca ctccatacaa tgaaatctgc ccatttcccg ggttcgatgg
    10861 aacctttatc gttttcccgg aaggagcccc gtgcggccca gatggtcatt cccctgaggg
    10921 tgttttcccg ggaaacggcg ttttccgtct gccagcctcc gggaggattg aaattgcgat
    10981 ccattctgga cacggcagca aaaaaggtat agagcggatt gatgctttcc accggggcat
    11041 cggtcccgaa aggcgcccat ccggcagcac agatcagttc cctgaaggca taggcgcctt
    11101 tgatcctttc aggtccaaga cgtgcgacag cccagcccat gtcggcagtg gcatgtgtgg
    11161 attgcacgga aggcactatg ttcagctcac caaaacgttt aaaatcatcc ggtgccacta
    11221 cctgggcatg ttctatccgc cagcgcagat cattgcccgg ctccaggaac tgcccgtata
    11281 agtccaatac ctttcccaca cctttgtcac caatggcatg tgtggctacc tggaatcccc
    11341 tttcataggc tttttcgcag gcagccaaaa aattccggtc cgatgtgaac tgtatgccct
    11401 tgttgagggg atcgtcggag tacggttcat acagcagggc tccgcttgaa ccaagggctc
    11461 cgtcaatata caatttcacc gcactgatat ccaacctgtc ggattggtag gggcgggtaa
    11521 aataagagaa gttttcttca gaaggatcca tccagacgtc gattctgagt ttaagggcac
    11581 ctgctttctg taacgaatcc agcaatttga tacggccaag gggcaatccg gcatctgtta
    11641 cagatccaag gccgaaagct aaacagagat cctgcgcttc caggatacat gcgaccaatt
    11701 cctggtcact catgtgtgga atggtggcat tgatccggtc tgaggtattt tccaggaata
    11761 cgcctgtgaa ttgtcccgag gtattcataa gggcttctcc ctgcgggatg gaaatatcgc
    11821 ctgcatggag accggccaga gcgatagcca cttcattgac ccaggtcgca tgaaaatcaa
    11881 ccctgcgcag gtaaacagga atattgggga acgccttgtt cagtttctca ttgtcaggaa
    11941 gatccttgtt ttcccataat gtctgatccc agccatcacc caccaggaaa ccgggatggt
    12001 gcagatcgta atgtttttgc aagcgggcta tcacttcatc cacagaggct gccccacgca
    12061 ggtcgacata acgcaatccc tgggcaacct ggaacaaatg gctgtgcgca tcgttgaatg
    12121 ccgggtatac cggggctccg ttaagatcaa gttcccggcg gcctttgaac tgttcccgga
    12181 tctggcctgt tgtcccactg gcgataaaaa tgccgtcatc cactgcaaag gcctcgcagg
    12241 cggaatagga gctgtcaacg gtatacaccc tggcattgta cacgaccagg tcaacgtgtc
    12301 tgcgcgtggt gcaggatgtg gaaaggatca gtgtcattcc agtaacaata agagctatcg
    12361 tttgtttatg catatcagat ctgttttctg agccgggcaa ttgggatatt gagctgttcg
    12421 cggtattttg ccactgttct tctggccact ttatagcctt tttcattcag gcaggcaacc
    12481 agctcctcat cggtaagagg cttgtgttta tcctcctgtt caatgctctg gaccaggata
    12541 ttcttgattt cacgcgtgga cacctcttca ccggattctg tcatcaggcc ctccgagaaa
    12601 aaatatttca gggaatatat gccgaagtgt gtctgtatgt atttggagtt gacaaccctg
    12661 gatatggtag agatgtcaag ccctgtaatc tcggcaatat tcttgagtac catgggttta
    12721 agcttcgtct catcaccttc tgcaaagtag tccttctgga aatccaggat ggctttcata
    12781 gtattgaaaa gggtattttg acgctgtttg agagcttcaa taaaccattt tgcagagtcc
    12841 agcttctgct tcacaaacgt tgcagcctct ttgctctccc ttgtactttg accggcggcc
    12901 ttgagaagca tgtcggcata ccgtttgttg atgcgaagct caggaacaga aaaacggggc
    12961 agggacaaca cgggttcgcc ctctttgatt tcaagcataa aatccggcac aacctgttga
    13021 gccctgtccg tataggaatc ttcaacatta ccgcccggtt tcgggttcaa ccgcaggatc
    13081 tcctctatgg cttccttgag ctcatcctct gtaatgccaa gcctggcaat gattttatcg
    13141 taatgctttt tggtgaactc ggcaaaatgc ttttccaaaa tcgtatgagc cgtttccacc
    13201 gccggttttt gttccttgtt gcgcagctgg aggagcaggc attcctgcag gtcccgtgcg
    13261 cctacaccgg gaggatcaaa ttcctggatt atggaaagtg cctgttctat ttcccgtgga
    13321 ccggcttcca gccccaggcg gaaagccatg tcgtcactca gtgtttcgaa atcccttctc
    13381 agatagccat catcatccag gctcccgatc acaaagaggg caatttccct ttctttgtct
    13441 gtcatggaac ggaaacccag ctgggcttcc aggttctgat ggaaactttc cctgaccgaa
    13501 aaagtcggga actccttttt catatctcga gactggttat ttacatacaa tttataggaa
    13561 gggatggaat catcctgacc ggtgtattcg ctcagggaca actctccggc gctctcaggt
    13621 tcttcatcgc cctcttttgt ttccaataca gggttctctt ccagctcctt cctgactctt
    13681 tgatcaagtt ccagggcggg cagctccaac agcttgatgg tctgtatctg cagcggggat
    13741 aatttctgtt tcagctgtag cttttgttgt agtgactgag ataacattta cattcagttg
    13801 cttgctgcaa agatacacaa ttgcattctt tttgataact ttgcatgtgt aaaagttatc
    13861 cctgccgatg gaaccaatca ttcctcctgt atctattcat ttgctggaag aagaactgac
    13921 aagggaacgt ttcgtacggt attcttcccg cggagagaat aaattatttg acgtaaatgc
    13981 gcatgaagct cccaacacaa tgagggagat cgggcgactg cgtgaaattg cattccgtgc
    14041 tgccggaggc ggtacaggtt atgcctcgga cattgattca tacgatgtga gtcccgagac
    14101 tccctacagg cagcttgtgg tgtgggatcc aaaaagaaaa gagatactgg gaggataccg
    14161 ctacataatc ggtgcaggga tcagacccca gaacctggcg acatatccca tattcagctt
    14221 ctctgataac tttgtcaata attaccttcc tttcacaatt gagctgggca ggtctttcgt
    14281 acaacccaat taccagaatt ccaatgaaaa ccgctccgga ctgtacgccc tggacaattt
    14341 atgggacgga ttgggggccc tgatcgtacg ttacccgcac atgaaatatt tcttcgggaa
    14401 agtgaccatg taccgtactt ataacaaaca ggccaggaat tgtctattat attttctaaa
    14461 caaatatttt cccgatcccg actccctggc aacagccatt gatcccctgg actataatgc
    14521 tgaaaaaccg tattacagga atattttcgt ggcgggcaat tatgatgatg actacaagac
    14581 attggtcagg gaaatacgta cgtacgggga aaggataccc ccgctgatca attcctatat
    14641 gaagatttct cccaacatgc gtgtttttgg cactacactc aatgaacatt tcggaggagt
    14701 ggaagaaaca gggatcctta tcaccatcaa agacatgtat ccggacaggg tagggcgaca
    14761 catgaattct ctcaggcatt ttgccgagag tgtaggtctc agatggtgga acaaagacgc
    14821 gtcaaagaag gctcccgaag atcagagtgg cggataagca atggcttccc ttgtaatgaa
    14881 agcctgggga tacgttttgc ggacctccat catgaaacgc atggcttcgc ttcttgtccg
    14941 gaaatcccct acagttactt taaaataagg gttttcatag atcctgtaaa cggcagtatg
    15001 cggatacatg gccatgaacg acatggcgat ggtttccgat tcagtcctgg ccgtttgctt
    15061 gttgtcaaaa aagatcctta cacggaagcc ctgcattttt ttttgagaga acctgttttg
    15121 ttctgcctgc cggttgacct tgagcctgat ggaatctgac tggattatcc tgaggcggtt
    15181 tccctcggga cccggtcttt gaaaatactc aaatacgtct gttccctgtg acagggtatc
    15241 cgggacctgt gccagggcgg catcagaaaa gtaacaacct gtgagcagca ccagtatggc
    15301 ggcaagaaca aatctattca taaatcaagc aagtttttgg ccgggatatc gttaagctgc
    15361 agtttacgtt tgatgccccc gctttttacc gttgcatcta tatccagccg gatctgacgg
    15421 agcaatgttt cgtccaggtc aacagcccct gagaacagta cagacatatc attgattctt
    15481 agtgataaaa gcccctcgtt tgtagaggtg ccccgggcgc tgaatactaa agccgaatcc
    15541 attgaaaatt gcgctatacg cttgtctttc agtcttatga tcccattcat tcccgtgagc
    15601 cggatcttgc cccctgtggg gttgttcagc gttgcgctga tttttacccg ggctgtcgac
    15661 aaactggtca tgttgaactg tctgacggca atattttcaa tagctatgtc ccggtagcgg
    15721 gaacaaccca ctgccatcat aatggccagt atacaaagga taaggctttt cctgttcatg
    15781 aggcaaaggt atacatttag ggtgaatatg ctatatttgt gccatgattc ccttttatat
    15841 agaaacttat ggttgccaga tgaatgtcag tgattcccag gtggttgcca gcatcctgtc
    15901 agacaatgga tacgaatcgt gcgacagctg ggaagcagcc tgtctggtgc ttatcaatac
    15961 ctgttccata agagaaaacg cggaaaaacg cgtcatgtcc cgtcttgatt tttttgcccg
    16021 ggtcaaacgc aaaaatccgc agctgaaggt tgccgtattg ggttgtatgg ccacaaggct
    16081 tggggactcc ctgcttatcc atccggcggt cgattgtgtt gccggtcccg acagctacag
    16141 gctgctcccc gcaatgctgg atacaccagg cagccagccc tcgcctcggc ccgggatgaa
    16201 gcaccccgta gcttcgtcaa atgtaaagta caatatcctg tccgaagacg gaatgcccgg
    16261 cgggccgctg cccgcccgca tgaacgttga gctttccgcc acggaaacgt atgcagatat
    16321 aaggccttta cggaacataa gctccggcgt gactactttt gtaaccatca tgcgcggatg
    16381 caacaacatg tgtgcttatt gcgtggtgcc ttatgtacgg ggcagggaaa gaagccggga
    16441 cccgaactcc atcctggaag aggtgaaaaa cctggtggac agcggctatc gtgaagtgac
    16501 actcctggga cagaatgtag actcctattt gtggcagcca aaggatggat ctgaacctgt
    16561 tcccttcgcc cggctgctgg aacaggtggc tttggtatcc ccattgctga gggtccgttt
    16621 cactacctcg catccgaaag acatgcagga cgatgtgctt atggccatgg cccgttatcc
    16681 caatatatgc aaacacatac accttcctgt gcaaagcggc agcaaccgca tattgcagaa
    16741 aatgaaccga aaatacacgc gtgaaggtta cctggaaagg attgcacgca tacgggagat
    16801 cttgcccggg tgtgccattt cctcggatat gatcgccggc ttttgctcag aaacggaaga
    16861 agaccacgcc atgacactaa gcctgatgga aacagtgcat tatgaccagg ccttcatgtt
    16921 ccagtactcg cagcggcccg gcaccctagc agcgcgtcac taccccgacg atgttccgga
    16981 agaggtaaag acacgcaggc ttgccgaggt cattgccctg cagaaccgcc tttccctgca
    17041 aagcaaccgg gccgatgtgg gaaaaatctt tgaagtgctt gccgagggcg tctccaagcg
    17101 ctcatcccgg gcctttttcg gccgcaccac cacaaacaag gtctgcgtat tcccaagaga
    17161 aaacagggcc ataggggatt atgtccgggt gttggtaaaa tcatgtaccg ccgcaacact
    17221 taaaggagaa attataaatg aaaaccaata aatcatgaca aaaatcaaag caacctacct
    17281 gggagacctc catgtagagg ccatccacct gcaatcggga tcccggattg aaacagatgc
    17341 tccggttgat aaccagggaa aaggcgaact tttttctcct accgatatgt ttgccacatc
    17401 cctggcagcc tgtatgatga ccatcatggg aatctcggcc cgaagttatg gttttgccct
    17461 ggaaggggcc tatgccgaag tggaaaaaat catggcctct tccccccgac gggtgggcga
    17521 aatcatcata gacctgtatt tccccgacgg taaaacttat ggcgaccggg aaaaaaggat
    17581 catagaagct gctgccgcca cttgtcctgt tgccaattcc gtgcatccgg acctgaaaaa
    17641 aacagtacgg tttcattact aagaaaaatt agctacattt gctgcccccg tttaccaaaa
    17701 ttgcccggat ggcggaattg gtagacgcgc tggtctcaaa caccagtggc cgcaaggctg
    17761 tgccg
//
LOCUS       DAOSOF010000026        17661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3229_length_17661_cov_4.825968, whole genome shotgun sequence.
ACCESSION   DAOSOF010000026 DAOSOF010000000
VERSION     DAOSOF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_17661_cov_4.825968"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLM68_02735"
     CDS             <1..324
                     /locus_tag="PLM68_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT54314.1"
                     /translation="DLAYAAADLVVSRAGAGSISEFCAAGKACIFVPSPNVAENHQTH
                     NAMALVKELAGRLVTDKDAPAILMENAMELIFNDSELKRLSENIEKLSRPHAAAHIAS
                     MVLEL"
     gene            321..1184
                     /locus_tag="PLM68_02740"
                     /pseudo
     CDS             321..1184
                     /locus_tag="PLM68_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
     gene            1266..1694
                     /locus_tag="PLM68_02745"
     CDS             1266..1694
                     /locus_tag="PLM68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOT54315.1"
                     /translation="MDVRHAGPNCWYIDDYAHHPGEIRASVLSVREWFPGEKITGIFQ
                     PHLYTRTRDFAGEFAKSLDLLDQVVLMPVYPAREAAIPGVDAELIAGKMDLQDVTILD
                     ASRIIDFVRENKPRVLITLGAGNIDRLVAPITECLKEMQL"
     gene            1670..2482
                     /locus_tag="PLM68_02750"
     CDS             1670..2482
                     /locus_tag="PLM68_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54316.1"
                     /translation="MPKGDAVMSMWKKALIISGYVALTGILGAYFYFATILVCDKNQE
                     RCQEVLIIVKDSVFNPLIRAGEVASFLDRSSVALLGRDFSAIDMYHLEKKVESFASVR
                     KCNAVRTIDGTLKLEILQHEPLFRIETQVGSFFVSKEKFIFPIVNPFRAPVLIVRGNV
                     PFSYPASYRGQMDSTDSWLASMYRMTGFITTHDFWSRKTETVVVENNQDIRIVPKEDD
                     LVLKIGDLSRFAYKLDKLQEFYRVLAPLGGKEKYSVVDARFGDQLVCTQRKD"
     gene            2509..3828
                     /gene="ftsA"
                     /locus_tag="PLM68_02755"
     CDS             2509..3828
                     /gene="ftsA"
                     /locus_tag="PLM68_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT54317.1"
                     /translation="MEKIISVIDLGTTKVVCLAGKKTAEGYKVIASCESPSQGIMRGE
                     VVNNQSVLNTLKPLLVELETRIGEPILEVEVGIAGRYIRCKSVSSNINRPDPNEVISE
                     QEINNMWRNMYNSRVDPGEEVLHVVPQSYHVDDYRSVPDPVGMYGSCIEANYKLFIGK
                     SVSAEHTKRTIEGTELKLNRLVLEPLATARAVLQEDEKELGVAMVDIGGGTTDLLVYQ
                     DNIIRHTAVIPFGGNVITEDIRQGCEVTVRQAEQMKIQYGSCYADLAPENKAIVIAGI
                     GGRESREIPFRFLANIIEARMDEILDAVMYEINQSGYGNRLPAGIVLTGGGSLLFNLP
                     QFIKFKTGYDARVARPHGIITEGCEEVNQVTYSCAAGLLMYGFENQGHFPEEKQADLT
                     EIFAGVNAGQTSKTKKTVKKVSPGKKPKEKTSIGNLLNSIFDVSNEA"
     gene            3834..5240
                     /gene="ftsZ"
                     /locus_tag="PLM68_02760"
     CDS             3834..5240
                     /gene="ftsZ"
                     /locus_tag="PLM68_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT54318.1"
                     /translation="MKEKEDEFIPLNWENNKSIIKVIGVGGGGGNAVSHMYRSGLSGV
                     DFVVCNTDAQALDLSPVPDKLQLGNTLTRGRGAGCNPELAKQAALESEARIKALFEGP
                     AEMVFITAGMGGGTGTGAAPVIAKIAKEMDLLTVAVVTLPFRDEGRDFLKRALDGIQE
                     LQQYTDSMIIIDNEKIYRSYGDLPIFEAFQRADDILKTAVKGISEIITCPGFINVDFA
                     DVKMVMTQSGMALMGTGSGSGKDRALKAVESAFTSPLLNDADLTRSRNVLINITSGKE
                     KALSMTELSQIMGYIADYTGGASNFKRGVLCDPSIQDEIRVTVIATGFSIHSLPPIAV
                     YGNDTEKEKAKKVSVYLDGESAQNEHEDPLPGLVVSPAALGNGDFKVTNNPRVFQVEP
                     VAVPAPAVLNKKPVLILEPNQNIAELEQIPAYKRRQSGHTMTNPSEIAKPSPNKVEEK
                     NGQQWLSADNAYIHQTQD"
     gene            5242..6795
                     /gene="gltX"
                     /locus_tag="PLM68_02765"
     CDS             5242..6795
                     /gene="gltX"
                     /locus_tag="PLM68_02765"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT54319.1"
                     /translation="MATTKVRVRFAPSPTGPLHMGGVRTALFNYLFARQQGGTFVLRI
                     EDTDMQRYVPGAEEYIIESLRWCGIMPDEGIDASGKVVTEPSPLHPHAPYRQSERKGL
                     YEKYARQLVDSNHAYYAFDTPHELESYREEAESRGQTFIYNHAFRKKTVNSLNMDPEE
                     VSRRLEENKNWVIRFKVPENKMVAMHDLIRGEVSVQTNTLDDKVLWKAQDGLPTYHLA
                     NIVDDYLMRITHVIRGEEWLPSLPLHYLLYEAFGWQDQRPLFSHLPLLLKPDGKGKLS
                     KRDGDRLGFPVFPLEWTSPSGEKSRGYREDGYYPEAFVNMIALLGWNPGTEQEIFSME
                     ELIALFSLDRVIKSGARFNPEKAKWFNQQYLRNRSDKQLAQDFMPLLRNRGISTTPPK
                     AEQVVSLIRNRADFVQDFWDLSYYFFIPPASYDPVMQKKYWNPEIPPVMLRAREVLER
                     CVPFDAGSIEQALHRWIEEEGLKTGQVMNALRLCLVGKPQGPGLSDIMSVIGKEDTLG
                     RIDNAINIL"
     gene            6797..8002
                     /locus_tag="PLM68_02770"
     CDS             6797..8002
                     /locus_tag="PLM68_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54320.1"
                     /translation="MSGKPRWWPLLVTNFLGVFNDNYLKTLACFIAVSWVGTEYEAPL
                     VSLAAGSLVVPYVFLSPLAGRWAVVFNKLSVVRIAKLAEVPIMLLASAGFLVHSVTLV
                     MVSILLMGVQSSLFSPSKYGLIRDIGGQENITFGTGNMEMVSFLGMITGTMMASFLAQ
                     SVPAWGLSLTFMAIAAGGWVSSLSIRAKETPPEENDRTIRPILFMVQSIGQARKYRGL
                     NRVILALSVFWLIAGMIQMTLLVYCRRNLGMSDLETGFVLTAAAVGTGMGCFFSGIVS
                     EKVDNRLLVPLSGLSITLLFLFMYFFPVRGFFFGFLFFITGILCGFYKVPFDAAIIRK
                     VPGRNLGHMLGYSNQMSFLFILAASAVFAIISRYAETRAVFLFLGTLMGLTTLFVVFS
                     KYLWSTKQE"
     gene            8002..8535
                     /gene="rfbC"
                     /locus_tag="PLM68_02775"
     CDS             8002..8535
                     /gene="rfbC"
                     /locus_tag="PLM68_02775"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOT54321.1"
                     /translation="MVFEETAIKGIWIITPRVIVDKRGYFMESYLKEELEKRVGPVDF
                     VQENESLSHFGVTRGLHFQKEPYAQSKFIRVVDGEIFDVAVDIRENSHTRGRCFHLHL
                     SSENKKQLFIPKGFAHGFCVLTPRAIVQYKTDERYNPASEGIYRYDSPALGIPWPLSP
                     GQMILSSRDREAQPFVL"
     gene            8539..9270
                     /gene="truB"
                     /locus_tag="PLM68_02780"
     CDS             8539..9270
                     /gene="truB"
                     /locus_tag="PLM68_02780"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT54322.1"
                     /translation="MLVLDRYTLSGPVPGPETVLEEGMIICMDKPLEWTSADVVRKIR
                     FRLQRRWHIKKIKVGHTGTLDPLATGLLIICTGKATRLADTMQAQDKEYVAGITFGAT
                     TPSCDLEHEIDAVYPWEHIDRNSLEKALEGFLGEQLQVPPVYSAKVIDGMRAYEFARQ
                     GMETPEMKKNPVQIHLLEIKDYVPPLLTLRIKCSKGTYIRALARDLGVALGSGAYLSS
                     LRRSASGPYKAEDAISIEEFETFFG"
     gene            9334..10383
                     /gene="queA"
                     /locus_tag="PLM68_02785"
     CDS             9334..10383
                     /gene="queA"
                     /locus_tag="PLM68_02785"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT54323.1"
                     /translation="MKLSQFNFDLPHSLIASRPATNRDESRMMVLNKKTGALDHMVFK
                     DIITLMQEHDVVIFNNTKVFPARLHGNKEKTGAEIEVFLLRELNKDQRLWDVLVDPAR
                     KIRIGNKLYFGENDALVAEVIDNTTSRGRTLRFLCDGTYDNFKTTLLRLGEMPIPKWI
                     RKHTEPFDQERYQSVFAKVEGAVAAPDGALHFSKELLKRMAIKGVISTEITLHMGMGH
                     FRTVDVEDLSKHKMDSEPVVISPSTAQIINHAKASGNRVFATGIDVLRAVETHVTTKR
                     EVTAFEGWTNKFIFPPYDFYIPNALITNFHLPQSTMLMFVAAFGGYDTVMKAYKTAVK
                     EKYHFGAYGDAMLII"
     gene            10406..11518
                     /gene="dprA"
                     /locus_tag="PLM68_02790"
     CDS             10406..11518
                     /gene="dprA"
                     /locus_tag="PLM68_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT54324.1"
                     /translation="MICGEEEFSICALSLCMRYEGGLARRLYDHYGSARTVFSRPLPE
                     LVCHKGMRRSIVEKMTDPSLPEQVSDVLQWLRSVNIRTVFLYDDEYPYRLRECPDAPL
                     LLYVAGQSVLNEGPVLSVVGTRKATPYGKALCREIISGFKPLGIKPVIVSGLAFGIDI
                     TAHRAALENDLTTVTVLPTGIDTIYPPAHLDIALRILERGAVLSEFPPKTPGNRKNFL
                     QRNRIIAGLADATLVVESKADGGAQITVHHALGYARDVLAVPGRPSDVCSQGCNNLIR
                     MNKAALVTSAREIAEAMQWEVSGKELQVAQQELFDSLDDKDRQIIVHIRENGGDINDM
                     GESLNIPFPELSARLVQLQVKGAVDCHDNKEYNSLI"
     gene            11515..12282
                     /locus_tag="PLM68_02795"
     CDS             11515..12282
                     /locus_tag="PLM68_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT54325.1"
                     /translation="MIDTHVHLYDQAFDTDRDLVVERAKNTGVRHCILPAIDKSSFRA
                     QQECLARYPWFCHKASGLHPTSVNTDFRKELDFAYSNLEGAVAIGEVGLDGYWSREFM
                     EEQILALEEQVQWAREKDLPLIIHSRSSFPELLSTLKKCRYPGMRGILHAWSGSAEVF
                     REANRYGHFLMGIGGVITYSNARLAGVVQEFDLAQIVLETDAPWLSPVPFRGKRNESA
                     YLKYIAEKLAQLKDVSAEKVVGQTTQNALSLFFGVKK"
     gene            12279..13310
                     /locus_tag="PLM68_02800"
     CDS             12279..13310
                     /locus_tag="PLM68_02800"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HOT54326.1"
                     /translation="MNTSSILVIYTGGTIGMKAHPMTGELVPFRFEHVLEEVPELRKF
                     GFRIDTHSFDPVIDSSDIFPEFWIRLALLIEGQYDKYDGFVVLHGTDTMSYSASALSF
                     MLENLEKPVIFTGSQLPIGLPRTDGKENFISAVEIAAEKDREGHAIVPEVCIFFQDEL
                     FRGNRTFKYNAEEFRAFRSPNHPPLAEAGIHIKYNHKYIRYPDTWGKKLRIHTRLGTD
                     IAILKIFPGTEKRVVEAVLGIEGLRAVILETYGQGNAPSVKWLLELLSAAVSKGIIIL
                     NITQCPAGSVSMNSYACGARLQEAGVLSGYDMTTEAAVTKIMLLTGLFSDNVRVKYFL
                     SQSLNGEFS"
     gene            13376..13464
                     /locus_tag="PLM68_02805"
     tRNA            13376..13464
                     /locus_tag="PLM68_02805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13411..13413,aa:Ser,seq:gga)
     gene            13531..14268
                     /locus_tag="PLM68_02810"
     CDS             13531..14268
                     /locus_tag="PLM68_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT54327.1"
                     /translation="MKKLFVFLAVLGFITISAQYAAAQTETPTGEIEQVEIPIHQQLK
                     TKYIEGGPGFMSAILLCLVFGLALGIERILYLSLSETNTSKLLAKLDDAMKEGGVPAA
                     MEICRNTRGPVASIFYQGLSRVDQGLEVVEKSITSYGSVQMMELEKNFSWIQLFIAIA
                     PMLGFLGTVIGMVSAFDAIESAGDISPTIVAGGIKVALITTVFGLIVAIILQLLYNYL
                     ISKLDGIVSSMEDASISMIDILVKYEK"
     gene            14278..14703
                     /locus_tag="PLM68_02815"
     CDS             14278..14703
                     /locus_tag="PLM68_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54328.1"
                     /translation="MKKLPKIALWVLFLISIVITVLYVINIKSENLDAWTYTYLNWAY
                     VTVGLTILAIILMPILTFKQRTVKLRSILLVVGLTVVLVGGSYLLAPGTPVTLANGKV
                     FEGSVVKLTDAGMYLTYILVACAVIAIIGGSVYNALKKY"
     gene            14718..15299
                     /locus_tag="PLM68_02820"
     CDS             14718..15299
                     /locus_tag="PLM68_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT54329.1"
                     /translation="MARPAGDINAGSMADIAFLLLTFFMMVTTMDTETGIQRRLPPMP
                     DENQIEQAEQINKRNIMVVLINNNDRLFAGGQEMPIVMLKEKVKEFLTNPANLPNLPE
                     KREKEIEGFGPYMVSRALVSLQNTRGTSYKAYIEVQNELVKAFNEVRDEFAMQNFGKK
                     YDLLDEDQQRIARDAVPMNLSEREPRDVTKRRR"
     gene            15311..15796
                     /locus_tag="PLM68_02825"
     CDS             15311..15796
                     /locus_tag="PLM68_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT54330.1"
                     /translation="MAKFRSKNSKKKVPALSTASLPDVVFMILFFFMISTSFRTSERV
                     VSVILPEATEIAKLERKDLASYINIGPPIPALQAQYGTEARIQLNDAFSTIEDIRDFI
                     AASRESMSEADRPLMTVTLKVDEDVRMGIVTDVKQELRRCSALKIMYSTRRKAAEQKG
                     F"
     gene            15878..17086
                     /locus_tag="PLM68_02830"
     CDS             15878..17086
                     /locus_tag="PLM68_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOT54331.1"
                     /translation="MKRLFTMLAVAVLMGCQQSVPQAKYVFYFIGDGMGLAQVALAEA
                     WLADSTDVIGFEKLSFTEFPVLGTATTFSASNIITCSSAAGTALATGTKTNNGMLGVS
                     PDSVSLEAITYKIKKAGYRVGVTSTVTLDHATPGSFYANASSRSDYYDIAVQLPQTGF
                     DFFGGGGFIQPEGEDKDQVSVLSLIEEAGYQVVYGIEQVDALAGAEKMLLLQDKERDF
                     MDALPYAIDRKESDMTHADIISVAVDFLDNEKGFFLMSESGRIDWACHSNDGKTLILE
                     ILDLNDAVQVAVEFAQKHPGETLIVVTADHETGGLALGLENGYSVYFDALAEQNASKD
                     ILLQARDMELVKQVREMNFKARIGWTTSSHTGIPVPVYATGSGSELFSGRMDNTDIPK
                     KICQAMGIPF"
     gene            17201..>17661
                     /locus_tag="PLM68_02835"
     CDS             17201..>17661
                     /locus_tag="PLM68_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOT54332.1"
                     /translation="MEILDKQEPDSPVNRGRRKNFSGGIFVGLFMILAGAILIFSNAG
                     WISWEARHILLSWQMFLIVIGLISLVRKQVTQGVILLVIGTIFLLPRIGALHIFPGME
                     WFTKLNWGNLWPIFLVAVGILIFTKAKKGDQHSNSHRSGNQAWHHRPSHEST"
BASE COUNT         4734 a         4162 c         4503 g         4262 t
ORIGIN      
        1 gacctggcgt acgctgcggc agacctggtg gtctcgcggg caggggcggg cagtatctcg
       61 gaattctgcg cagctggaaa agcctgcatt ttcgtaccct cacccaacgt ggcagagaac
      121 catcagacac acaatgccat ggcactggta aaagaactgg caggcaggct tgtgactgac
      181 aaggacgcac ccgccatact gatggagaac gccatggaac tgatattcaa tgacagcgaa
      241 ttgaagagat tgtcagaaaa catagaaaaa ttaagccgcc cccatgcggc tgcacacata
      301 gcatcaatgg tactggaatt atgacagaaa acgtatattt tctgggtata ggaggaatcg
      361 gaatgagtgc gctggcaaga tactacaagc atgccggggc ttctgtatgg ggttacgacc
      421 tttccccttc cgggctcaca cgaaggctgg aagaggaagg catgggaatc cattaccagg
      481 atgatccgtt gcaaataccc agtcaggtag cagagaacag ggataagacc ctggtgatct
      541 atacaccggc cctcccccgg gaccacaggg agttgcaatg gttcagggaa aaagattacg
      601 aaattatcaa gcgctctgtc gccctgggac acctgtctga aaatcatgtc accctggcag
      661 tggcgggaac gcacggaaaa accaccacct ccaccctgct tgcccacatt ctgcatcatt
      721 cgggaacagg gtgcactgct ttcctgggag gcatttccaa gaattacgac acaaacctct
      781 tgctgggcaa cagtcctttt cttgttgccg aggcagatga atacgaccgt tgattcctcc
      841 agctgcaccc ccaaagcgcc ctgatcacat ctgccgatgc agaccacctt gatatctacg
      901 ggacccacca ggctgtcagg gaagcttact ctcaattcgc ttcccaggta gtatcggggg
      961 gtacgctagt gatcaaaaaa agcgttgaca ttgctttgcc cttacagccc ggagtaaccg
     1021 cctacacata ccatagcagt gagccatgtg atttttacgc cctgaatcac accatgctga
     1081 aagggggatt ttactctttt gacctgatgc tcaaaggtaa aaaagtggag ggatgccgcc
     1141 tggacctgcc gggaaggatc aacgtggaaa atgctgtcgg ggcctcggct atggcctgtc
     1201 tggccggtat tgctccggat gccattaagg atgccctggc atcattcagg ggggtatcca
     1261 ggagaatgga tgttcgtcac gctggcccga attgttggta tattgacgat tatgcacatc
     1321 atccgggaga aatccgggca tcggtccttt ctgtcagaga gtggttcccg ggagagaaaa
     1381 tcaccggaat cttccagcct cacctgtata caaggaccag ggattttgcc ggggaatttg
     1441 ctaaaagcct tgacctgctt gaccaggtgg tgctgatgcc tgtttatccg gcaagggagg
     1501 cagccattcc gggcgtggat gcggaattga ttgccggaaa gatggacctg caagatgtga
     1561 ccatactgga cgcgagccgg atcatagatt ttgtaaggga aaacaaaccg cgggttctga
     1621 taaccctcgg ggcgggaaat attgaccgtc ttgtggcacc cataacagaa tgcctaaagg
     1681 agatgcagtt atgagcatgt ggaaaaaagc actgataata tccggatacg tcgcattgac
     1741 aggtatcctg ggtgcttatt tctattttgc cacaatcctt gtttgcgaca agaaccagga
     1801 aaggtgccag gaagtgctga tcatcgtgaa ggacagtgtg ttcaatccgt tgataagggc
     1861 cggggaagtg gcatctttcc tggacaggag ctctgttgca ttgctgggca gggatttctc
     1921 cgccattgac atgtatcatc tggaaaagaa agttgaatca tttgcttcgg taaggaaatg
     1981 caatgctgtc aggaccatag atggcacact caagcttgag atactccagc atgaaccgct
     2041 cttcaggatt gaaacgcaag tgggttcctt ttttgtgtca aaggaaaagt tcatatttcc
     2101 cattgtcaat ccattccgtg caccggtcct gatcgttcgc ggcaatgttc cgttttcgta
     2161 tcccgcatcc tataggggac agatggattc gacagactca tggctggcat cgatgtaccg
     2221 catgaccggc tttattacga cccatgactt ctggagcagg aaaacagaga ccgttgtggt
     2281 ggaaaataac caggacatac gcatagtacc caaagaagat gacctggtac tgaaaattgg
     2341 tgacctgagc cggtttgcct acaaactgga taagttacag gagttttaca gggtacttgc
     2401 acccctggga ggaaaagaga aatacagtgt tgtggatgcg cgtttcgggg atcagctggt
     2461 ttgtacgcaa agaaaagatt aagaaaaaat aaataactta acatacctat ggaaaaaatt
     2521 atatctgtaa tcgatttggg gaccaccaag gtggtatgtc ttgcagggaa aaaaacagca
     2581 gagggttata aggtgatcgc ctcgtgtgaa tcaccctcac aggggatcat gaggggagaa
     2641 gtggtgaaca accagagcgt gctcaacaca ctcaaacccc tgctggtaga actggaaacc
     2701 aggataggag aacccatcct ggaagttgag gtgggaatag ccggccggta catccgttgc
     2761 aaatcggtta gcagcaatat caaccgaccc gacccgaacg aggtgatatc ggaacaggaa
     2821 atcaacaata tgtggcggaa catgtacaac tcaagggtgg atcccggtga ggaggtcctg
     2881 cacgtggtcc cgcaatcgta ccacgtggac gattacagaa gtgttcctga tccggtaggc
     2941 atgtacggta gctgcattga agcaaactac aagcttttca taggaaaaag cgtctctgcc
     3001 gagcacacca agagaaccat tgaaggaaca gaactgaaac tgaaccgcct tgttctagag
     3061 cccctggcaa ccgccagggc tgtgcttcag gaagatgaaa aagagctggg agtggccatg
     3121 gtggacatag ggggcggtac taccgacctg ttggtctacc aggacaatat catccgacat
     3181 accgcggtca ttcctttcgg aggcaatgtc atcacggagg acatccgtca gggatgcgag
     3241 gtgacagtgc gacaggcgga acagatgaaa atccagtatg gttcatgtta tgcagacctg
     3301 gcccccgaaa acaaagccat tgtcattgcc gggatcgggg gacgtgaatc ccgggagatt
     3361 cccttccgtt ttctggccaa cattatcgag gccaggatgg atgagatcct ggatgcggtg
     3421 atgtatgaga tcaaccagag cggctacgga aacaggctcc ctgccggcat tgtcctgacc
     3481 ggaggcggat cgcttctttt taaccttccc caattcataa aattcaaaac aggttacgac
     3541 gcacgtgttg cccgtcccca cgggatcata accgagggat gtgaggaggt caaccaggtt
     3601 acctattctt gcgccgcggg attattgatg tacggatttg agaatcaagg ccattttcct
     3661 gaagaaaagc aggctgacct gaccgagata tttgccggtg tcaatgccgg gcaaacatct
     3721 aaaacaaaaa aaacggtgaa aaaggtttct ccgggcaaaa agccaaagga aaaaacgagt
     3781 atcggcaatc ttttgaatag tatttttgat gtatcaaacg aagcgtgagt ataatgaaag
     3841 aaaaagaaga tgaattcatt cccctgaact gggaaaacaa caaatccatc attaaggtga
     3901 taggagttgg tggcggaggc gggaatgctg tcagccatat gtaccgctcg ggattgagcg
     3961 gcgtggattt tgtagtatgc aacaccgatg ctcaggcgct ggatttgagc ccggtacccg
     4021 ataaactgca attgggaaac acactcacgc ggggaagagg agccgggtgc aaccccgagc
     4081 tggccaaaca ggctgccctg gaaagcgagg cccgtataaa ggccctgttt gaaggaccgg
     4141 cagaaatggt attcattacg gcaggcatgg gaggcggtac cggaacggga gctgcccccg
     4201 taatagccaa gatagcaaaa gaaatggatc tgttgaccgt tgccgtggta accctgcctt
     4261 tccgcgatga gggaagggat ttcctgaaac gggcgctgga cgggatacag gaattgcagc
     4321 aatatacaga ttccatgatc atcatcgata acgaaaaaat atacaggtct tatggcgacc
     4381 tcccgatttt tgaggcattc cagagagccg acgatatatt aaaaacagcc gtgaagggaa
     4441 tatccgagat cattacgtgc ccggggttca tcaatgtcga ttttgccgat gtgaagatgg
     4501 tcatgacaca aagcggcatg gcccttatgg gaacaggctc gggatcgggc aaagacaggg
     4561 cgctgaaagc cgtcgaatcg gcattcacct cgcccctgct caacgacgcc gatctgaccc
     4621 gctctcggaa tgtgctgata aacatcacct cgggaaaaga aaaagcatta tccatgaccg
     4681 aattgtccca gataatggga tatattgccg attatacagg gggtgcttcc aatttcaagc
     4741 ggggagtcct ttgcgatccc tccatccagg acgagatacg ggtaacggtg atcgctaccg
     4801 ggttcagtat tcattcactt cctccgatcg ccgtttacgg aaatgataca gaaaaagaaa
     4861 aagcaaagaa ggtttctgta tacctggacg gggaatctgc ccaaaacgaa cacgaggatc
     4921 ctttaccggg gctggtagtc agccctgcag ccttgggcaa tggtgatttc aaggtcacaa
     4981 acaatccccg tgttttccag gtagagcctg tggccgtccc cgccccggct gtcctcaaca
     5041 aaaagcctgt cctgatcctg gaaccgaatc agaacatagc agaactggaa caaatcccgg
     5101 catacaaacg cagacagtcc ggtcatacca tgacaaatcc ctccgaaatc gccaaacctt
     5161 ccccgaacaa agtagaggaa aaaaacggcc agcagtggtt gagtgccgac aatgcataca
     5221 tacaccaaac acaagactga tatggcaaca acaaaagtca gggttcgttt tgccccaagc
     5281 cctaccgggc cgctccacat gggaggcgta cgtaccgcgc ttttcaatta cctttttgcc
     5341 aggcaacagg gcggtacctt tgtactccgt attgaagaca cggacatgca acggtatgta
     5401 cctggagcgg aagaatatat cattgaatca ctccgctggt gcggtatcat gcccgatgaa
     5461 ggcatagatg cctcaggcaa ggtggttact gaaccatcac cgcttcatcc ccatgcccct
     5521 taccggcaga gtgaacgcaa aggcctttat gaaaaatacg cccgtcaact ggtcgacagc
     5581 aaccatgcct attatgcctt tgatacaccg catgaactgg aatcataccg ggaagaagcc
     5641 gaaagccggg gtcaaacgtt catttacaat catgcattca ggaaaaagac agtcaattcc
     5701 ctgaacatgg atccggaaga ggtgtcacgc aggctggagg aaaacaagaa ctgggtcatc
     5761 cgtttcaaag ttcctgaaaa caagatggta gctatgcacg atctgatccg cggcgaggta
     5821 agcgtacaaa ccaacacact ggatgataaa gtgttgtgga aagcacaaga cggtctgccc
     5881 acctaccacc tggcaaacat agtggacgat tacctgatgc gcataaccca tgtgatccgc
     5941 ggggaagaat ggctcccttc gctacccctg cattatttgc tgtatgaagc tttcggatgg
     6001 caggaccagc gccccctgtt ttcacatttg cccctgttgc tgaaacccga tggaaaggga
     6061 aaactgagca agagggacgg agaccggctg ggattccctg tatttcccct ggagtggacc
     6121 agcccttccg gagaaaagtc caggggatac cgtgaagatg gttactaccc ggaagccttt
     6181 gtgaacatga tcgctcttct gggatggaat ccgggcaccg agcaggagat cttttccatg
     6241 gaagaactta ttgccttgtt ctccctggac cgggtgatca aatctggtgc ccgcttcaac
     6301 ccggaaaaag ccaaatggtt caatcagcaa tacctgagaa accgctcaga caagcagttg
     6361 gcacaggatt tcatgccact gctcagaaac agggggatca gcactactcc gcccaaagca
     6421 gaacaggtgg tctctctgat ccggaacagg gccgattttg tacaggattt ctgggatttg
     6481 tcgtattatt ttttcatacc tcccgcatct tatgatccgg tcatgcaaaa aaaatactgg
     6541 aatcctgaaa ttcctcccgt catgctacgc gccagggagg tcctggaacg gtgtgtcccc
     6601 tttgatgccg gatccattga acaggcattg caccggtgga tagaggagga agggctcaaa
     6661 acggggcagg tcatgaatgc gttgagactg tgcctggtag gaaaaccgca gggtccggga
     6721 ctgtccgaca tcatgtcggt cattggaaag gaagacacac tcggccggat agataacgct
     6781 ataaacatct tgtagcatgt caggaaaacc ccgttggtgg cccctgctgg taacgaattt
     6841 ccttggggta tttaacgaca attatctgaa aacactagcc tgtttcattg ctgtttcatg
     6901 ggttggcact gaatatgaag ccccgcttgt ttcactggcc gccggttcac tggtggtccc
     6961 ttatgtcttc ctttctcccc ttgccggaag gtgggccgtg gttttcaaca aactttcggt
     7021 agtcaggata gcaaagcttg ccgaggtccc catcatgctg ctggcctcgg cggggttcct
     7081 ggttcacagc gtcacgctgg taatggtctc catcctgctg atgggggtgc aaagttccct
     7141 tttctcccct tccaaatacg ggttgattcg ggatatcgga ggccaggaaa acattacctt
     7201 cggaacgggg aacatggaaa tggtttcttt cctgggcatg atcacgggaa ccatgatggc
     7261 ttccttttta gcccaaagcg ttcctgcctg gggactttcc cttactttca tggcaatagc
     7321 agccggagga tgggtcagtt ccctgtccat cagggcaaag gaaacacctc ccgaagaaaa
     7381 cgacaggacc atccgtccta tactgttcat ggtgcaaagc atcgggcagg caagaaagta
     7441 ccggggtctg aaccgggtga tcctggccct gtctgtcttt tggcttattg ccggaatgat
     7501 ccagatgaca ttgcttgtgt attgcaggag gaatttaggc atgagtgacc tggagacagg
     7561 ttttgtgctt acggcagctg ccgtgggaac cggcatgggt tgtttctttt ccggtattgt
     7621 ttcagagaaa gtggacaata gattactggt tcccctgagc ggtctttcca tcaccctgtt
     7681 gttccttttt atgtatttct tcccggtcag aggatttttc tttggctttt tgttctttat
     7741 caccgggatc ttatgtggtt tttacaaggt gccttttgat gcggccatca tcaggaaggt
     7801 tcctggacgc aacctgggcc atatgctggg ctactccaat caaatgtctt ttcttttcat
     7861 actggccgct tcggccgtct ttgccataat cagccggtat gcagaaacac gtgccgtatt
     7921 cttgttcctg ggaacgctca tgggacttac cacccttttt gtggtttttt ctaagtatct
     7981 ttggagcaca aaacaggaat gatggtattt gaagaaactg caataaaagg tatctggatc
     8041 atcaccccgc gtgttattgt cgacaagagg ggttatttta tggaatcgta ccttaaggag
     8101 gaactggaga aaagagtcgg gcctgttgat tttgtacagg aaaatgagtc cctatcacat
     8161 tttggtgtaa ccaggggcct tcatttccag aaagagccct atgcccagtc caagtttatc
     8221 cgcgtggtgg acggtgagat ttttgatgtg gctgtggata taagggaaaa ttcacacacc
     8281 aggggtcgct gttttcatct gcatctgagc tcggaaaaca agaagcagct attcatacca
     8341 aaaggtttcg cccacggttt ttgtgttttg actcccaggg ctatagtcca gtataagaca
     8401 gacgagcgct ataatcccgc ctcggaagga atatatcgtt atgattctcc tgccctgggg
     8461 attccatggc ccctttcgcc cggccagatg atactttcat caagggaccg ggaggcacaa
     8521 ccctttgtat tgtaaaaaat gctggtactt gacagatata cattatccgg tcctgttccc
     8581 ggaccggaga cagtgcttga ggaggggatg atcatttgca tggacaaacc cctggaatgg
     8641 acatcggcag atgtggtcag aaagatcagg ttccggctcc agcgtcgctg gcatatcaaa
     8701 aaaatcaagg tgggacatac cgggacactt gatcccctgg ccaccggcct gctgatcatc
     8761 tgcacgggca aggctaccag gctggcggac acaatgcagg cgcaggataa ggaatatgtg
     8821 gccgggatca ctttcggggc cacaactccg tcctgcgacc tggaacacga gattgacgct
     8881 gtatatccct gggagcatat cgacaggaac tccctggaaa aagccttgga aggttttctt
     8941 ggagaacaac tccaggtacc accggtctac tcggccaagg tcattgacgg catgcgggcc
     9001 tacgagtttg ccagacaagg gatggaaacg ccggaaatga aaaagaatcc ggttcaaatc
     9061 catctgctgg aaataaaaga ctatgtgccc ccactgctta ccttacgcat caagtgcagt
     9121 aaaggcacat atatccgtgc tctggcaagg gatctgggtg tggcactggg cagtggcgcc
     9181 tacctgtcct ctctgcgccg ttcggcttct ggtccttaca aagcagagga tgcgatctcc
     9241 atagaagaat ttgaaacttt cttcggctaa ttgaaacttt tttgtttttt ttacgtataa
     9301 tacgtcgctt atctatttac aaaaaacgac tgtatgaagc tttcacaatt caatttcgac
     9361 ctgcctcatt ctcttatcgc cagccgtccg gcaaccaacc gtgatgaatc ccgcatgatg
     9421 gttttgaata aaaaaaccgg tgccctagac catatggtat tcaaggatat cataaccctg
     9481 atgcaggagc atgatgttgt aatcttcaat aatacaaaag tgtttcctgc gcgccttcat
     9541 ggaaataagg aaaaaaccgg agccgaaatt gaggtgttcc tgcttcgtga actcaacaaa
     9601 gaccagcgcc tgtgggatgt tctggtagat ccggcacgaa agatccgcat tggcaacaag
     9661 ctgtattttg gtgaaaatga cgcattggtt gccgaggtga tcgacaacac cacatcacgt
     9721 ggaaggacac tgcggttcct gtgcgacggt acttacgaca atttcaaaac caccctgttg
     9781 cggctgggag aaatgcctat tcccaaatgg atccgtaaac atacagagcc ttttgaccag
     9841 gaacggtatc aatctgtttt tgccaaggtg gagggagcgg tcgctgctcc cgacggcgct
     9901 ctccatttca gcaaggaact tctcaagcgc atggcgatca aaggggttat cagcacggaa
     9961 ataacactgc acatgggcat ggggcatttc cggacagtag acgttgaaga cctgtcaaaa
    10021 cataaaatgg attcggaacc ggtggtgata tccccatcca ccgcacagat cattaaccat
    10081 gcgaaagcgt caggcaaccg tgtttttgcg acagggatag atgtgctccg ggctgttgaa
    10141 actcacgtga ctaccaagag agaagtaacg gcattcgagg gctggaccaa taagttcatt
    10201 ttcccccctt acgatttcta cattcccaat gcactgatta ccaatttcca tcttccccaa
    10261 tccaccatgc tcatgtttgt ggctgccttt ggaggttatg atacggtcat gaaggcttat
    10321 aagacggcag tcaaagaaaa ataccatttc ggagcgtacg gcgatgccat gttgattatt
    10381 tagtcgattt ccactatatt tgggaatgat ctgtggagaa gaggaattca gcatttgtgc
    10441 cctgagtctg tgcatgagat atgagggagg tctggcacgg aggctgtatg atcattatgg
    10501 cagcgcccgc accgttttca gccggccgct cccggaactg gtctgtcaca aggggatgcg
    10561 aagaagcatc gtggaaaaaa tgaccgaccc ttccctccct gaacaggtat cggatgtttt
    10621 gcaatggctg agatctgtca atatccggac cgtcttcctg tacgatgacg aatatcccta
    10681 caggctgcgg gaatgccccg atgccccgct gcttctatac gtggcgggac aatccgtgct
    10741 caacgaaggc cctgtcttgt cggttgtggg aacgcgtaaa gccaccccgt atggaaaagc
    10801 cctctgcagg gagatcatat ccggtttcaa acctttgggg atcaagcctg taattgtgag
    10861 cgggctggcc tttggaatag acataacagc gcacagggca gctctggaga atgacctgac
    10921 aacagtaacc gtcctgccta caggtattga caccatctat cccccggctc acctggatat
    10981 tgccctccgg atcctggaac gtggtgccgt gttgagtgaa ttccctccca aaactcccgg
    11041 gaaccgcaag aatttcttgc agcggaacag gatcattgca ggactggcag acgccaccct
    11101 ggtggtggaa tcaaaagccg atggaggggc tcagatcacc gtgcaccatg cattgggtta
    11161 tgcaagagat gtgctggctg ttccgggcag gccttccgat gtttgctcgc agggatgcaa
    11221 caaccttatc aggatgaaca aggcggcgct tgtaacctcg gcccgggaaa tagcagaggc
    11281 catgcaatgg gaagtgagtg gaaaagagct gcaggtagcg caacaagaac tctttgattc
    11341 cttggacgat aaggataggc agattattgt ccacatccgg gaaaacggag gagatatcaa
    11401 tgacatgggg gaaagcctga atatcccttt tcccgaactt tcggcccgtc ttgtacaact
    11461 ccaggtgaaa ggcgcggtag attgtcatga taataaagaa tacaactcac tcatatgata
    11521 gacacacacg tacacctata cgaccaggca tttgacacag acagggacct ggtggtggaa
    11581 agggcaaaga acacaggcgt acgccattgt atactcccgg ccatagacaa aagcagcttc
    11641 agggcccagc aggagtgcct ggcgcgttat ccgtggttct gccacaaagc ctcgggcctc
    11701 caccccactt ctgtcaatac agatttcaga aaagagcttg atttcgcata cagtaacctg
    11761 gaaggcgccg tagccatagg agaagtggga ctggacggtt actggtcaag ggaatttatg
    11821 gaagaacaga tcctcgccct ggaagaacag gttcaatggg cccgtgaaaa ggacctgccc
    11881 ctgatcatac actcccgttc ttcgttccct gagctgctgt ccacgttgaa aaaatgccgg
    11941 tatcccggca tgcgagggat ccttcatgcc tggtctggaa gcgcggaagt cttccgggaa
    12001 gcaaaccgtt acggacattt tctgatgggg atcggggggg tgatcactta cagcaacgca
    12061 cgcctggcgg gggtggtgca ggagttcgat ctggcccaga tcgttctgga aaccgatgcc
    12121 ccctggctaa gtccggttcc cttccgcgga aagaggaatg aaagcgctta tttaaaatat
    12181 attgccgaaa aactggctca gttgaaggat gttagtgctg aaaaagtagt tggacaaacc
    12241 acgcaaaatg cattatcttt gttttttggc gtaaaaaaat gaatacatcg tccattctcg
    12301 ttatttacac gggaggaacc ataggcatga aagcacaccc catgaccgga gagttggtcc
    12361 cctttcgttt tgaacatgtc ctggaagaag tcccggaatt gagaaaattc ggattcagga
    12421 ttgacaccca ttcctttgac cctgttattg attcttccga tattttccct gaattctgga
    12481 ttagactggc ccttcttatt gaagggcaat acgacaagta cgatggtttc gttgttctgc
    12541 acgggaccga caccatgagc tattctgcgt ctgccctgag tttcatgttg gaaaatctgg
    12601 aaaaaccggt catttttacc ggcagccagc ttcccattgg attaccgagg acagatggca
    12661 aggaaaattt catatcagcc gttgagatag cagccgaaaa agacagggaa ggacacgcca
    12721 tagtccccga agtgtgcata ttcttccagg acgaattgtt tcgcgggaac aggaccttca
    12781 agtataatgc agaggaattc agggctttcc gctcaccaaa ccaccccccc ctggcagaag
    12841 ccggaattca tataaaatat aaccacaaat acatccggta tccggacact tggggaaaaa
    12901 agctccggat acacacccgg cttggaacag acattgccat tctcaagatt tttccaggca
    12961 cagaaaaaag ggtggtggaa gccgtcctgg gcattgaagg tttacgggca gtgatcctgg
    13021 aaacctacgg acaggggaat gccccgtctg taaagtggtt gcttgaattg ctctctgcag
    13081 ctgtaagcaa ggggatcatc atcctgaaca ttacccagtg cccggccgga agcgtctcca
    13141 tgaattcgta tgcatgcggt gccaggcttc aggaagctgg cgtactgagc ggttacgaca
    13201 tgacaacaga agcggctgtg acaaaaatca tgttattaac aggtcttttt tctgataatg
    13261 tgcgtgttaa atattttctt tcacagtctt taaatggcga attttcttaa atatgcttgg
    13321 attaaaactt ttttctgtaa cttgcgctac tttttgtaaa tacttaatta taaaaggaga
    13381 ggtggccgag ttggtctaag gcgcgcgcct ggaaagtgcg taaactccaa aagggtttcg
    13441 cgggttcgaa tcccgccttc tccgcaagca ggaaaaaata aagaaaacgt ttgactacta
    13501 taaattctta actctaatta ttcaacagaa atgaaaaaac ttttcgtatt tttggcagtt
    13561 cttggattca ttaccatctc tgctcagtac gctgccgcac aaacagaaac cccaacgggt
    13621 gaaattgaac aggttgagat acccattcac cagcagctta aaacaaaata cattgagggt
    13681 ggtcctggtt ttatgtcagc tatcctgctc tgtcttgtat tcgggcttgc acttggaatt
    13741 gaacgtattc tctatctgtc tctttctgaa accaacacct cgaaattact ggcaaaactg
    13801 gatgatgcca tgaaagaagg cggtgtacct gcagccatgg agatctgccg taatacacgc
    13861 ggacctgtag ccagcatttt ttaccagggg ctcagccgtg ttgaccaggg cctggaagtc
    13921 gtagaaaaat caattacttc ctatggttct gtacaaatga tggaactgga aaaaaacttt
    13981 tcatggatcc agctgttcat cgccattgct cctatgttgg gattcttggg aacagttatc
    14041 gggatggtca gtgcatttga tgctatcgag tctgccggag acatttcccc caccatcgtt
    14101 gccggcggta tcaaggttgc ccttatcact acggtgtttg gtcttatcgt ggctattatc
    14161 cttcaattat tatacaacta cctgatttca aaactggatg gcattgtaag tagtatggaa
    14221 gacgcatcca tatccatgat tgacattttg gttaaatatg aaaaataaga cattgcgatg
    14281 aagaaacttc caaaaatagc attatgggta ctctttttga tcagcattgt cattactgtg
    14341 ttatatgtca ttaacatcaa gagtgaaaac cttgatgcat ggacctatac ttatttgaac
    14401 tgggcctatg tgactgtagg actgacaatt cttgcgatca ttctgatgcc aatacttacc
    14461 tttaagcaaa gaacggttaa attacgctcg atcttgctgg tggtaggtct gactgttgtc
    14521 ctggtaggag gttcatacct tttggctcct ggcactcctg taaccctggc aaacggaaaa
    14581 gttttcgaag ggtctgtagt aaaattaacc gacgccggta tgtacctgac ctatattctg
    14641 gttgcttgcg ctgtgattgc cattattggc gggtctgttt ataatgccct gaagaaatac
    14701 taataaaatt aaacactatg gcaagaccag caggtgatat taatgcaggc tcaatggcag
    14761 atatagcttt cctgcttctt actttcttta tgatggttac gaccatggat accgaaaccg
    14821 gtattcagcg ccgccttcct cccatgcccg atgagaatca aattgagcaa gccgagcaga
    14881 tcaacaaacg aaacattatg gttgtactta tcaacaacaa tgaccgtttg ttcgcggggg
    14941 gacaggaaat gcctatcgtg atgctaaagg aaaaagtcaa ggaattcctg acaaatcccg
    15001 caaatctgcc caatttacca gaaaagcggg aaaaggaaat tgaaggcttt ggcccttata
    15061 tggtatcgcg tgcacttgtt tcgctgcaaa acacccgagg gacttcatac aaagcctata
    15121 tcgaagtaca aaacgaactg gtaaaagcct tcaacgaggt ccgtgacgag tttgcgatgc
    15181 agaattttgg aaagaagtac gacttgctgg atgaagatca gcagcgtatt gcaagggatg
    15241 cggttccaat gaacctttcc gaaagggaac caagagatgt aacaaaaaga agaagatagg
    15301 aggcaagact atggcaaaat ttagaagtaa aaacagtaag aaaaaagttc ccgccctgag
    15361 tacagcctca cttcctgacg ttgtgttcat gatattgttc tttttcatga tctcaaccag
    15421 tttccgtact tcagaacgtg tggtttcggt aatattaccg gaagccactg aaattgcaaa
    15481 actggaacgc aaggacctgg ccagttatat aaatattggc ccccccatcc ccgcattaca
    15541 ggcacaatac ggaaccgaag caaggataca gctgaacgac gctttcagca ccatagagga
    15601 tatccgcgac tttattgcag cttctcgtga atccatgtca gaggctgacc gtcctttaat
    15661 gacggttacc ctgaaggtgg atgaagacgt gcgcatgggg attgtgacgg atgtcaagca
    15721 ggaactccgc cgttgttctg ctttgaagat catgtactct acccgccgca aggccgcaga
    15781 acaaaaaggt ttttaatccc ttatatctat caatgaaagc cgtagccagc actacggctt
    15841 tttttataac tttacaaccg attcttaatt catacctatg aaacgtttat ttaccatgct
    15901 ggcagttgcc gtattgatgg gttgtcagca atcagtacca caggcaaaat atgtatttta
    15961 ctttattggt gacgggatgg gacttgccca ggttgccctg gcagaagcct ggctggctga
    16021 ttccacagat gtaatcggat ttgaaaagct gtcctttacg gaattcccgg ttctcggaac
    16081 agccacaaca ttcagcgcca gcaatatcat aacttgttcc tctgcagccg gtactgcttt
    16141 ggccaccggc acaaaaacca acaacgggat gctgggtgtt tcccccgact ctgtcagcct
    16201 tgaagcaata acctataaaa ttaaaaaagc aggttaccgt gtcggtgtca catctactgt
    16261 gacattagat catgctacac cggggtcttt ctatgcgaat gccagctcca ggtctgatta
    16321 ttatgacatt gctgttcaat tgccacaaac gggatttgac ttttttggcg gaggaggctt
    16381 tatacagccc gaaggagaag acaaagacca ggtatcggtt ctctctctga tagaagaagc
    16441 aggataccag gtcgtctatg ggattgagca ggttgatgca ttggccggag cagagaaaat
    16501 gctgctgctc caggataagg aacgggactt tatggatgcc ctgccctatg ccattgaccg
    16561 gaaggaaagc gacatgacgc atgccgacat cataagcgtt gcggttgatt ttctggacaa
    16621 tgaaaaagga tttttcctga tgagcgaaag cggccgtata gattgggcct gtcactccaa
    16681 tgacggaaaa acattaatcc tggaaatcct ggacctgaat gatgcggtcc aggttgccgt
    16741 tgaatttgca caaaaacatc ccggggagac cctgatcgta gtgactgcag atcatgaaac
    16801 aggaggcctg gcacttggcc tggaaaacgg gtattccgtg tattttgatg ctctggcgga
    16861 acagaatgcc tctaaagaca tactgctgca agcccgggat atggagctgg tcaagcaggt
    16921 gagagagatg aacttcaagg cccgtatcgg gtggaccact tcatcccata ccgggatacc
    16981 ggttcctgta tatgcaacag gatcaggaag tgaacttttt tcgggcagga tggataatac
    17041 tgatattcca aagaagattt gccaggcaat gggcattcct ttttaaccgg gaaaaccatc
    17101 cgttcgtcgg gccgatacct tgcattcacc gatttcctgt tgccaaaaga gaggtttgcc
    17161 gttacatttg caacagtaca actttaaaat ttttacaatt atggaaattc tggacaaaca
    17221 ggaacctgat tccccggtca acaggggacg caggaagaat ttttccggag ggatattcgt
    17281 aggtcttttc atgattcttg ccggagctat cttgattttt agcaatgccg gatggatatc
    17341 atgggaggca cgccacattc tcctctcctg gcaaatgttt cttattgtta tcggacttat
    17401 ttccctggtc agaaagcagg tcacacaagg tgtcatattg ttggtaatag ggaccatctt
    17461 cctgttgcca cgcataggcg ccctgcatat tttccccggt atggaatggt ttacaaaact
    17521 caattggggc aatttatggc ctatattcct ggtggccgtg ggtatattga ttttcacaaa
    17581 agccaaaaaa ggcgatcagc acagcaacag ccacaggtcc gggaatcaag cctggcatca
    17641 ccgcccttca cacgaatcca c
//
LOCUS       DAOSOF010000027        16920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3410_length_16920_cov_5.347406, whole genome shotgun sequence.
ACCESSION   DAOSOF010000027 DAOSOF010000000
VERSION     DAOSOF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16920
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_16920_cov_5.347406"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1482)
                     /gene="rmuC"
                     /locus_tag="PLM68_02840"
     CDS             complement(259..1482)
                     /gene="rmuC"
                     /locus_tag="PLM68_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT54333.1"
                     /translation="MDAVSIVAGLSVGIIAGGVIVWGLINRRLQAKDLEVKAKEQQIG
                     EKEREIRELGAGMASARTALDLKEKELADTQKQLIVQFENLANKILEANTLKFEETSK
                     QSMQQLLKPLGEQLVDFKSRVDAAHIEDTKQRSTLEERIRGLIEQTNQVSAQANNLAE
                     ALKGSPKTQGNWGEMVLERILEQSGLEKDTHYMMQESFRNEQGNVLYPDVVIHLPGER
                     NIIVDSKVSLVAYDRYVSATSQQVQQQALQEHIMSIKKHVDELAAKQYDSFQGSLDFV
                     MMFVPVEPAYLIAIQNDPGMWSYAYHKRIVLISPTNLIACLKLMSDLWSREMQNKNAM
                     QIARRGELMLKKFENFIETLSALGGTIAKSQEAYDKAMGQLRDGQGNLIGQASKLREL
                     GIKAFKDLPEMIAED"
     gene            complement(1523..2929)
                     /gene="xylE"
                     /locus_tag="PLM68_02845"
     CDS             complement(1523..2929)
                     /gene="xylE"
                     /locus_tag="PLM68_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HOT54334.1"
                     /translation="MKNMDKGSKAYLGSLTFVAVLGGLLFGYDTAVISGAERALEAFF
                     SQATDFVYTKGWHGITASSALIGCIIGGFLSGLLAPRWGRRNSLRFGAVLFFISAIGS
                     WRPEMFFFEFGKPTFSLLLAFNAYRILGGIGVGLASAICPMYIAEMAPAHVRGRLVSV
                     NQFAIIFGMLVVYFVNYLIRANLPEPIEVSMAEIGWRRMFLSEAYPIALFGFLLMFVP
                     KTPRFLVMSGNETKALHVLTRINGTNAARTILEEIKETVVEKREKLLSYGVTVVIIGI
                     LLSFFQQAVGINVVLYYAPRIFENLGASGDVSMRQTVVMGFVNINFTLVAIFTVDKFG
                     RKPLLIIGSIGMMIGMAALSVLSFMDVIGIAALVFIIIYTASFMMSWGPITWVLISEI
                     FPNTIRGQAVAIAVAAQWIANFLVSSTFPSLSEWSTGGAYLIYAFFSLLSGIFVYKMV
                     PETKGKTLEDMTRLWRKK"
     gene            complement(2943..4259)
                     /gene="xylA"
                     /locus_tag="PLM68_02850"
     CDS             complement(2943..4259)
                     /gene="xylA"
                     /locus_tag="PLM68_02850"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOT54335.1"
                     /translation="MATKEYFPGIGKIKFEGKDSRNPLAFRYYDPEKVVYGKRMKDWF
                     KFAMAYWHTLCADCHDQFGPGTINREWWQSKDPVTAAKEKMDAGFEFMQKLGIEYYCF
                     HDVDLVEEGATVAEYEARMKEIVAYAKKKQKETGIKLLWGTANVFGNKRYMNGAATNP
                     DFDVVARAIVQIKTAIDATIELGGTNYVFWGGREGYMSLLNTDMKREKHHLGIMLQKA
                     RDYGRAKGFKGTFLIEPKPMEPTKHQYDTDVETILGFLKEYGLEKDFKINIEVNHATL
                     AGHTFEHELQCAADAGILGSIDANRGDYQNGWDTDQFPIDIWEVTQAMLVVLQAGGLN
                     NGGVNFDAHIRRNSTDLQDLVIAHIAGMDTFARGLEAAAAILEQSPYLKMRKTRYASF
                     DAGIGKQFEEGKLDFQAIYEYGSKLPEPRQISGQQELYETIVSMYI"
     gene            complement(4272..5756)
                     /locus_tag="PLM68_02855"
     CDS             complement(4272..5756)
                     /locus_tag="PLM68_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT54336.1"
                     /translation="MKSIGIDVGTSFIKVSLLDVETGRCQASYSFPSAEMPVKAVRAG
                     WAEQDPRIWWENVGWCLKEMNGKHGLKDVKSIGITYQMHGLVCIDEKGNPLRDSIIWS
                     DSRAVEYGAEAFLHIGKEKCLSRLLNSPGNFTAAKMAWVKENEPELFKRTCKFFLPGD
                     YIAYKLTGNISTTESGLSEQILWDFTDDAPAGFVMDHFGFPAHILPEHKPSLGIQCHT
                     SAGTEKLFGIPAGTPVSYRAGDQPNNAFSLNVMEAGEVAATAGTSGVVYGVTDKSIAD
                     QRSRVNQFLHVNHRPGDPHYGVLLCINGTGILNAWIRRNLLPGMSYDQMNEKASQAPA
                     GCDGLHVLPFGNGAERILENRFTGASFSGIDLNRHDTRHFLRAAQEGIAFSFRYGMDI
                     MKQMGIVPTVIRAGKANMFLSPVFRATIATLANASIELYNTDGSLGAARGAALGAGLY
                     QSREACFSGLELLQTEKPQPLWKERLEEAYGHWKEQLDKQLSNI"
     gene            complement(5737..6474)
                     /locus_tag="PLM68_02860"
     CDS             complement(5737..6474)
                     /locus_tag="PLM68_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT54337.1"
                     /translation="MQTNQSISVDCVVFGFDGLSLRCLLVRRNGLDYKLPGSLILENE
                     DIPSAAYRVLEEMTGLRDVFLKELSVFSNPSRVGKEDLEWINAFYGVNTRRVVTVAYY
                     ALVKLDGNMRSYTEEKGAIWKEVSCIASLAMDHKQILHKALDMLHRDMTYSPIAFELL
                     PRKFTIRELAGLYEKILGVEIDSRNFRKKIFSCGYILPAGDKEKGVPHKPAAYFVFDK
                     NRYKKEEKKTSKLNFINWNEYYEKHRN"
     gene            complement(6568..9402)
                     /locus_tag="PLM68_02865"
     CDS             complement(6568..9402)
                     /locus_tag="PLM68_02865"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HOT54338.1"
                     /translation="MMTLAGSAELAGRSREEYIMRHPVENGFALAREGEPAPMWVSGQ
                     DYPGVLRVAGHVQADMEKVTGNRPLLFTGSDPAPCERMIIAGTLGKNGLIDQLVRSGR
                     ISGKWLKGKKETYVATVIQEPAEGIREALVIAGSDKRGTIYGLYDLSRQIGVTPLHFW
                     ADVPVPPAKDLYAMPGEYTPGEPAVRYRGIFINDEAPALSGWVHERYGAFNSRFYEHV
                     FEYILRMRGNFLWPAMWGRAFYDDDPENPRLADEYGIVIGTSHHEPMMRAHVEWARYG
                     TGGWNYQTNPLVLRNFWKEGMERKGDYESIVTVGMRGDGDEPMGTERNIDLLEQIIDD
                     QRRIIEQVTGKPARQTPQMWALYKEVQDYHDQGMDVPEDVTLLFCDDNWGNVRKLPVQ
                     GEKPRAGGYGMYYHFDYVGGPRNYKWLNTNQISRVWEQMDLCYRHGVDRIWVVNVGDI
                     KPMEFPIAFFLEMAWNPAAMTRQRMSAYPRTWATAHFGEEYAAEIAGLITAYTQLNSR
                     RKPELTNPGTYHLFHYREADRVVATYRQLENRALELYRNMEASYRDAFFQLVLFPIQA
                     CANLNDMYVSAAKNQLYAREGRAATNEMASRVEELFIRDSLLTACFHNGLAAGKWNHM
                     MSQTHIGYTYWQEPAVKRIPVPEAGRLNISPGTGSSPLPVLDPFGSDTLFFEVYNTSV
                     TPRRYLLKTGNSWLKVSSPQGSVDQQSAFFLTADWDKVPAGRTETYMELAQEPGLLQE
                     TICPERIAVTVYNPVVPETPATGAFFESNGHVAIEAMHYSRRVQEAPWVWEHIPDLGR
                     THSGMRIVTKRGDPEAVPGAIAELSYDIVFFSQGTVQVEALFSPTLNFHGHGLRYAIA
                     FDDEPFRTVDVHGQYNERVWETWVADNIIRSGTEHQILTPGLHTLRIRAVDPFLVLQR
                     IEIDAGGLLPSYLGPPESYHNARNLGGTRF"
     gene            complement(9462..11522)
                     /locus_tag="PLM68_02870"
     CDS             complement(9462..11522)
                     /locus_tag="PLM68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT54339.1"
                     /translation="MSRDVLITLLLIICTGSCRMTPHKEPVAALSLLHEDHYNAPVIL
                     PKILSDGLVLQRGKEIPVWGKAPAGAPVKVEFAGIEQYTRADGSGNWSVSFPAMDPGG
                     PYTLKVNGRVVHDILVGDVWLFSGQSNTELSVSRVAIRYPIEATTYSNTQIREFRAAT
                     HYNFQGPLWDIPAGRWKLCVQEEIPEFSALAFFFGMEWNQKHNIPVGLIVSAVGGSPL
                     DAWLDEATLRGFPGPTGPDHYPERLTALRRECWVDSVKQAETQAMAHYRSLFTPDPGP
                     EGSQGWVPYRVPSFWDQADATPSGPLSQSPGSAWFKKVFYLTETQIGHAQEGAPDQDG
                     MLILGTMTDADRTFLNGTQVGSTVYQYPPRIYTIPKGLLKAGSNELLIRIENYWGPGG
                     FTSGKDYALYIGAVHPFYAMADTVSLEGDDWMARMGSTLTNAGAEYPATTTWHYQPAG
                     LYNALLAPLTQLPVKGVIWYQGESETKRRDYGQMLSILIDRWREDWNDDALPFVIVQL
                     PNYMKDKAPWPMETDWANRRDEQLAAVRARKHTALVVTHDIGEWNDIHPLNKKELAHR
                     VVSAAEYLGAGNRKAPLSPLPQHVVLRGNRVVITFSHAGKGLITKDGPDPAHFALADV
                     QGRYFRASARIIGKKKVAVWCKEDFTPHGVRYAWANDPSEANLQNDAGLPAPTFQWNL
                     NDSK"
     gene            complement(11524..14112)
                     /locus_tag="PLM68_02875"
     CDS             complement(11524..14112)
                     /locus_tag="PLM68_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT54340.1"
                     /translation="MKRVSLIVLCLLSGLWSCKPAYQRENLSFQERAEDLVSRMTLKE
                     KVSQMTHESSAIERLGVPEYNWWNECLHGVARAGQATVFPQAIGMAAMWDKEMMFRIA
                     TAVSDEARAKHHDFLRQGKRGIYQGLTYWTPNINIFRDPRWGRGMETYGEDPYLTGEL
                     AVQYIKGLQGDDPKYLKLVATAKHFAVHSGPESTRHSADVHPNQRDFYETYAPHFKKV
                     VQEGGVWSVMGAYQRLWGEPCCGSFFLGSLLRDRWGFQGYVVSDCWAIKDFYDKGMHE
                     IVETPAEAAALGVKAGTDLNCGVTYPYLVEAVEKGYIEEALIDTAVQRLMLARFKLGM
                     FDKPRNVPYSKIPIEVVESPEHVSLAREAACKSIVLLENDGILPFTKEVNKVAVIGPN
                     ADDLEVLLGNYNGFSKHPVTPLQGIREKLPQAEVSFEQGCALAAGLPYFKPVPSGVLF
                     TDPQMAKPGLTGHYYDNSLWEGDPVHERTDKVVDFLWWNEAPFEDMNVRNYSVEWSGV
                     LCVPESGRYALGVEAFSGCTLTLDGEMLIKRIDEHQPRKEYEYVELEAGKPYALTLRY
                     VQDNTEYAVMRLLWEAPQRNLLEEALAVASESDLVVLCLGLSPLLEGEEMKVRVEGFN
                     QGDRLDTGLPVTQLELIKAVKKLGKPTVLVLMNGSAVSFDKEVKSLPAIIEAWYPGQE
                     GGNAIADVIFGDYNPSGKLPVTFYQSIDQIPAFEEYAMEGKTYRFFRDEPLYSFGYGL
                     SYTTFTYESLKMPESVPCGIPADISFRVTNTGERDGDEVVQVYVSLHDASNPVPIKSL
                     QGFERIHLKAGESRVVDFTIVPEQMASLDDNQVWVVEAGDLVLEVGGKRYTVRLEGDT
                     VFIPNE"
     gene            complement(14140..14763)
                     /locus_tag="PLM68_02880"
     CDS             complement(14140..14763)
                     /locus_tag="PLM68_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOT54341.1"
                     /translation="MKRFVVSLTVLLSLNVMYAQDITLPAPNKEGGKPLMEVFNNRRS
                     TREMSDKPLSQQTLSDLLWAAAGVNRPDGRRTAPSAMNMQEIDIYVYTPKAVYLYIPQ
                     EHILRHLLTGDFRAEIARQPFAQKAPVVLLYVANYERMTRLDQQQKDFYGATDTGFIS
                     QNVYLFCAQEGLNTVVLGMIDREAAKSRLGFDGKAQLGQPVAYPKQD"
     gene            complement(14768..>15704)
                     /locus_tag="PLM68_02885"
     CDS             complement(14768..>15704)
                     /locus_tag="PLM68_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOT54342.1"
                     /translation="WYIKEYGIAQVSMNITNISVTPLHVAFDEVCDKAYKRGIRVTGT
                     ELVGLVPKSTLLDAGRHFLHKQQRSSGLPEEEIIRIAIKSMGLDELKPFDPKTRVLEY
                     LLEDTSSEKGLCGMTCREFALETASESPAPGGGSVSAYMGALGASLGTMVANLSSHKR
                     GWDDQWEYFSQWAEKGQALIAELLNLVEEDTLSYNKVMEAFSSPCKDDLEKATLYAAQ
                     VPLQTMKTSLRVFDILEAMIGKGNPNSVTDAGVGAFAVLGAVKGGFLNVLINVGGLRD
                     KEKARMLKEEAQALLDLATEREHELWEKVCNKVKK"
     assembly_gap    15705..15804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15805..16560)
                     /gene="ftcD"
                     /locus_tag="PLM68_02890"
     CDS             complement(<15805..16560)
                     /gene="ftcD"
                     /locus_tag="PLM68_02890"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOT54343.1"
                     /translation="MKRIIECVPNFSEGRDKQVISRIADVISRVEGVTLFNVDPGEAT
                     NRTVMTFAGTPEAVVEAAFQAIKTASENIDMRKHHGEHPRIGATDVCPLIPVSGITME
                     QVVVYARDLARRVGEELGIPVYCYESAAFSEKRKNLAVCRAGEYEGLEAKMKDPLWKP
                     DFGPARYTERTASTGLSVIGARDFLIAVNFNLNTTSVRRANAIAFDVREKGRKVMVNG
                     QETWEPGTLKDCKAIGWYIKEYGIAQVSMNITNI"
     gene            complement(16565..>16920)
                     /locus_tag="PLM68_02895"
     CDS             complement(16565..>16920)
                     /locus_tag="PLM68_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT54344.1"
                     /translation="RWENIEILAVPAYNILNKRPDGQPFHPKGYGNGYVFTFGDVKVY
                     ISGDTELIPEMDNLGPIDVALLAKNLPYTMSDDQFVQAVKKIGAPMVYPVHYFELDKA
                     ALEKAVGEKVKLIYE"
BASE COUNT         3755 a         4594 c         4078 g         4393 t
ORIGIN      
        1 acctcatgtt tggcgggcgt tactgccgcg ggctgaaatg ggtgtctttg tcgccacctg
       61 gaacagagag cctcggcgta ccccaacaag ctctctgttc caggtggcga cagagacggc
      121 aggcagaaaa aaagcgggag tttctgcgta ccaaaaagct tcccgttctt gtggtgtcag
      181 cgatgactgc aggcggaaaa agaacagaga gccccggcgt accaaaagct ctctgttcca
      241 agtgggggtt gctgtcaatc aatcctctgc gatcatttcc ggcaaatcct tgaaggcctt
      301 gatgcccagt tctctgagct tactggcctg gcctatgagg tttccctggc catcccgcaa
      361 ctgacccatg gccttgtcgt aggcttcctg ggactttgct atggtgcctc ccagggcgga
      421 caacgtctcg ataaaattct cgaacttctt gagcatcaac tccccgcgcc tggctatctg
      481 catcgcattc ttgttctgca tttcacggct ccacaagtct gacatcagtt tcaggcaggc
      541 tatcagattg gtgggactga taagcactat gcgcttgtgg taggcatagc tccacattcc
      601 cgggtcattc tggattgcaa tcaaataggc cggctccacg ggcacgaaca tcatgacaaa
      661 atccagggaa ccctggaaac tgtcgtattg tttggcagcc agttcatcca cgtgcttttt
      721 gatggacatg atgtgttcct gcaaagcctg ctgctgcact tgctgtgatg tggcagaaac
      781 atacctgtca taagccacca gggaaacttt ggagtcaact atgatgttgc gttctcccgg
      841 caaatggatc accacgtcgg ggtagagcac attcccctgc tcattgcgaa aggattcctg
      901 catcatataa tgcgtgtcct tttccaggcc cgactgttcc aggatccttt ccagcaccat
      961 ttccccccag ttcccctgcg ttttggggct tcccttcagc gcctcggcca ggttgttggc
     1021 ctgggcgcta acctggttgg tctgttctat cagtccccgg atccgttcct ccagggtgga
     1081 gcgctgtttt gtatcctcaa tatgagcggc atctaccctg cttttgaaat caaccagttg
     1141 ttctcccagg ggcttgagca attgttgcat gctctgcttg ctggtttctt caaatttcag
     1201 ggtattcgct tccagtatct tgttggcaag gttctcaaac tgaacaatca gctgtttttg
     1261 cgtatcggcc agttccttct ccttgagatc aagggctgtc cgtgccgagg ccatgccggc
     1321 acccagttcc ctgatctccc tttccttttc cccaatctgc tgctctttgg ccttaacttc
     1381 cagatctttt gcctgtaatc gcctgtttat caatccccag acgattaccc cccctgcgat
     1441 gatccccacc gaaagaccgg ctactatcga aactgcatcc ataagtacaa agttagcaaa
     1501 aaagcgggta ccacacggca gctcactttt ttctccacag gcgggtcatg tcttccagtg
     1561 tcttgccctt tgtttccgga accatcttgt atacgaatat accggacagc agggagaaga
     1621 aggcataaat aaggtaagcg cctcccgtgc tccattccga aagcgaggga aatgtggaag
     1681 aaaccaggaa attggcaatc cactgggcag ccacggctat ggccacagcc tgtcctcgga
     1741 tggtgttggg aaatatctcg gatatcagca cccaggtaat cggaccccag ctcatcatga
     1801 aggaggctgt atagataatg atgaacacca gggcggcaat ccctatcaca tccatgaagg
     1861 agagcacgct cagggcggcc atgccgatca tcatgcctat ggagccgata atgagcaacg
     1921 gcttgcgtcc gaatttatct accgtgaaaa tggccaccag ggtaaagttg atgttgacaa
     1981 atcccatgac cactgtctgt ctcatggaaa catcacccga agccccgagg ttttcaaata
     2041 ttctgggggc ataatacagc accacgttga tcccgacagc ctgctggaag aaagagagca
     2101 ggatccctat gatcactacc gttacgccat acgacaagag tttttcccgt ttttcaacca
     2161 ccgtttcctt gatttcctcc agtatggtgc gggcggcatt tgtcccgtta atgcgggtca
     2221 gcacatgcag cgccttggtt tcattccccg acatgaccag gaaacgcgga gtctttggaa
     2281 caaacatcag caggaagcca aaaagtgcga taggataggc ctcgctaagg aacatccttc
     2341 tccagcctat ttcagccatg gacacttcaa tgggttccgg caggttagcc cggatcaggt
     2401 aattcacaaa atataccacc agcatcccga agatgatggc aaactggttc acggaaacca
     2461 gccggcccct cacatgggca ggtgccatct cggcaatgta catgggacat atggccgatg
     2521 ccagtccaac acctattccc cccaggatcc tgtatgcgtt gaaagccagc aggagggaga
     2581 aggttggttt gccgaattca aaaaagaaca tttcggggcg ccaggaacct atggctgaaa
     2641 taaaaaacag caccgctcca aatcgaaggg aattgcgtct tccccagcgc ggggccagca
     2701 aacccgacag gaaacctcca ataatgcatc caatcaaagc gctggaggcc gttatgccgt
     2761 gccatccctt ggtgtaaaca aagtctgttg cctgggaaaa gaatgcttcc agggctcttt
     2821 cagctcctga tatcacagcg gtgtcatacc caaaaagcaa cccgcccaat acggcaacaa
     2881 aagtaaggga tcccaggtat gccttgctgc ctttatccat gtttttcata ggtgcaccca
     2941 tcttaaatat acatgcttac aatggtctcg tacagctcct gctgtccgct gatctggcgt
     3001 ggttccggca atttggaccc atactcgtat atagcctgga aatccagctt gccttcttca
     3061 aattgtttac caatgcctgc atcgaaagaa gcataacgtg ttttcctcat tttaagatag
     3121 ggagactgtt ccaatatggc cgctgccgct tccagaccac gtgcaaaggt gtccataccg
     3181 gcgatgtggg caatgaccag atcctgcaag tctgtggagt tccttcttat gtgagcgtca
     3241 aagttcacgc cgccattgtt cagccctcct gcctgcagta ctacaagcat tgcctgggtg
     3301 acttcccaga tatcgatggg gaactggtcc gtatcccagc cattctggta gtcgccccgg
     3361 ttggcatcta tggatcccag gatgccggca tccgcagcac attgcagttc atgctcgaag
     3421 gtgtgtccgg ccagtgtggc atggttcact tctatattga tcttgaaatc tttttccaac
     3481 ccgtattcct tgagaaagcc caggatagtc tccacatctg tgtcgtactg atgttttgtg
     3541 ggttccatgg gtttgggctc aatcagaaag gttcctttaa atccttttgc ccttccgtaa
     3601 tcgcgggctt tctgcagcat gatgcccaga tggtgctttt cacgtttcat gtcggtgttc
     3661 aacagagaca tgtagccttc acgtccgccc cagaaaacgt aattggtgcc gcccaattca
     3721 atggtggcat ctatggctgt cttgatctgc acgatggcac gtgcaaccac atcgaaatcg
     3781 ggattggtag ctgccccgtt catatacctt ttgtttccga aaacgttggc tgtgccccac
     3841 agcagcttga ttcccgtttc cttttgtttt ttctttgcat aggctacgat ctccttcatg
     3901 cgggcctcat attccgcaac cgtggcccct tcttccacaa ggtccacatc gtggaaacaa
     3961 taatattcaa tgccgagttt ctgcatgaat tcaaacccgg catccatttt ttccttggca
     4021 gccgttacgg ggtcttttga ctgccaccat tcacggttaa tggttcccgg cccaaactgg
     4081 tcatggcagt ctgcacacag ggtatgccag taagccatgg cgaacttgaa ccagtctttc
     4141 attcttttgc catataccac tttttcagga tcatagtaac ggaaagccag cggattgcgg
     4201 ctgtccttac cttcgaattt gatttttccg atacccggaa aatactcttt tgttgccata
     4261 attataagtt tttaaatatt tgatagttgt ttgtcaagtt gttctttcca gtggccgtat
     4321 gcttcttcca gccgttcttt ccacaagggt tgcggttttt ctgtctgtaa cagttccaaa
     4381 ccggaaaagc aggcttcccg gctttgatac aagcctgccc ccaaggctgc cccgcgtgcc
     4441 gctcccaggg atccgtctgt attgtagagt tcaatggaag cgttggccaa agtggctatt
     4501 gtggccctga aaaccgggct caggaacata ttggctttcc cggcccggat cacagtgggt
     4561 actatgccca tttgtttcat gatatccatc ccgtaacgga aagaaaaggc tatgccttcc
     4621 tgcgctgccc gcagaaaatg tctggtgtcg tgacggttca ggtcaatgcc gctgaagctt
     4681 gccccggtaa aacggttttc gaggatcctt tccgccccgt tcccgaaagg aagcacatgc
     4741 agcccgtcac acccggcagg cgcctgggaa gccttttcat tcatctggtc gtatgacatc
     4801 ccgggtagca ggtttcttct tatccaggca ttcaggatac ccgttccgtt aatgcacagc
     4861 aacacgccgt aatgcggatc tcccggcctg tggttcacat gcaggaactg gttcacgcgt
     4921 gagcgctggt ctgctatgga tttgtcggta acaccgtaca cgacacctga agtgccggcc
     4981 gtagcggcca cttcccctgc ttccatcaca ttgagcgaaa aagcgttgtt tggctgatcc
     5041 ccggcacggt aggaaacggg agtgccggcg ggtatgccaa agagtttttc tgtcccggcg
     5101 gaggtatggc actggatgcc cagggaaggc ttgtgctcag gaaggatgtg agccgggaag
     5161 ccgaaatgat ccatcacaaa cccggcgggg gcatcgtctg taaaatccca caaaatctgc
     5221 tcggacaaac cgctttctgt ggtggaaatg ttgcctgtga gcttataggc gatatagtct
     5281 ccgggtaaaa agaatttgca ggtgcgtttg aatagttccg gttcgttttc ttttacccag
     5341 gccattttgg ccgccgtgaa attccccggg gaattcaaaa gccgtgaaag gcatttttcc
     5401 ttgcctatgt gaaggaaagc ctctgccccg tattccacgg ccctgctgtc actccagatg
     5461 atggaatccc tcagcggatt gcctttttca tctatacata ccagaccgtg catctggtag
     5521 gtgatgccta tggatttaac atccttaagc ccgtgtttcc cattcatttc tttcaggcac
     5581 caacctacgt tttcccacca tatgcgggga tcctgttctg cccatcctgc acggacggcc
     5641 ttaacgggca tctcggcaga aggaaatgag taactggcct gacaacggcc ggtttcaaca
     5701 tccaggaggg agaccttgat aaaagatgtc cctacgtcaa ttcctatgct tttcatagta
     5761 ttcgttccaa tttatgaaat tcagcttgct ggttttcttt tcttcttttt tgtatctgtt
     5821 cttgtcaaac acaaaataag cggccggctt gtgaggcact cctttttcct tatctcccgc
     5881 cggcaggatg tatccgcacg agaatatctt cttgcggaaa ttgcggctgt cgatttccac
     5941 ccccagaatt ttctcgtaaa gcccggccag ttcccggatg gtgaatttcc tgggcaataa
     6001 ttcaaaggct atgggtgaat aagtcatgtc cctgtgcaac atgtccaggg ccttatgcag
     6061 gatttgctta tggtccatgg ccagggaggc gatacagctg acttctttcc agatagcccc
     6121 tttctcttct gtgtaggatc gcatgttccc gtccagtttc accagggcat aataggcaac
     6181 ggtcaccacg cgacgcgtgt tgaccccgta aaacgcatta atccattcca ggtcttcctt
     6241 cccgacacgc gagggattgg aaaaaacgga caactccttg agaaaaacat cccgcagccc
     6301 tgtcatctct tcaaggaccc tgtatgcggc agaaggaatg tcctcatttt caagaatcag
     6361 acttcccggc aatttgtaat ccagcccgtt gcgcctgacc agaaggcacc tgagggacag
     6421 tccgtcaaaa ccaaacacca cacagtcaac cgatatggat tggtttgtct gcatattcaa
     6481 agtaaagaat aatttacttt aaatccaaac ttagcgtaat ttttacgcta agaaattatt
     6541 taattggtca ttcctggaaa attttttcta aaagcgtgtt cctcccaggt tccgggcgtt
     6601 gtggtagctt tccggtggtc ccaggtagga aggaagcagc ccgccggcat caatttcaat
     6661 cctttgcagc accaggaacg ggtccacggc cctgatgcgc agcgtgtgaa ggcccggggt
     6721 aaggatctgg tgttctgtcc cggaacggat gatattgtcc gccacccagg tttcccatac
     6781 ccgttcatta tactggccgt gcacatctac tgtacggaat ggttcatcat caaaggcaat
     6841 ggcataccga aggccgtgtc cgtgaaaatt cagggtgggt gagaaaaggg cttccacttg
     6901 aacggttccc tgcgaaaaga aaacaatgtc ataagaaagt tcggcaatcg ctcccggaac
     6961 agcttcggga tcaccccgct tcgtaactat cctcatcccg gaatgggtcc ttcccagatc
     7021 cgggatgtgt tcccagaccc acggggcttc ctgtaccctt ctgctgtagt gcatggcttc
     7081 tatggccacg tgtccgttgc tttcaaaaaa ggcacctgtg gcgggtgttt cgggaacaac
     7141 aggattatat accgttacgg ctatgcgttc cgggcagatg gtctcttgca gcaagcctgg
     7201 ctcctgtgcc agttccatat acgtctcagt cctgccggcg ggcactttat cccagtcggc
     7261 agtgaggaaa aaagcagact gctggtccac cgatccctgc ggtgaagaca ccttcagcca
     7321 ggaattgcct gttttcagta gataacgccg gggtgtgacg cttgtattgt atacctcgaa
     7381 aaagagggtg tcacttccaa agggatccag cacaggcaac ggagaagacc cggttccggg
     7441 actgatattg agcctgcccg cttcaggcac cggtatcctt ttcacagcgg gttcctgcca
     7501 gtaagtatat cctatatgag tctgtgacat catatggttc cattttccag cggccagacc
     7561 gttatggaaa caggcggtaa ggagggaatc cctgataaaa agttcctcta ctcgagaagc
     7621 catttcattg gttgcagccc tgccctccct ggcatagagt tgatttttag cagctgacac
     7681 atacatatcg ttcaggttgg cgcatgcctg tatgggaaaa agcaccagct gaaaaaaggc
     7741 atccctgtaa gatgcttcca tattccggta cagttccaga gccctgtttt ccagttgccg
     7801 gtatgttgca accactctat ctgcttccct atagtggaac aaatgatacg ttccgggatt
     7861 tgtcagctcc ggtttccgcc tgctattgag ttgtgtgtaa gcagttatca ggccggcaat
     7921 ttccgcggca tattcttctc cgaaatgagc ggttgcccac gtccggggat atgcactcat
     7981 gcgttgccgt gtcatggccg cgggattcca ggccatttcc aggaaaaagg caatggggaa
     8041 ttccatgggc ttgatatcgc ctacattgac aacccatata cggtctaccc catggcggta
     8101 acacaggtcc atctgctccc acacacggga tatctgattg gtgttaagcc acttgtagtt
     8161 tctcggcccg ccaacgtaat caaaatggta atacatgccg taacctcctg cccgtggctt
     8221 ctccccctgg acgggaagct tgcgtacatt gccccagttg tcatcacaga aaagcaaagt
     8281 cacgtcttca ggaacatcca tgccctgatc gtgatagtcc tgtacctctt tgtacagggc
     8341 ccacatctgc ggtgtctggc gggcgggttt tcccgtgact tgttctatga tccggcgctg
     8401 gtcatcaatg atctgctcca gcaagtctat gtttcgctcg gtacccatgg gctcgtctcc
     8461 gtctcccctc atccccacgg taacgatgct ttcatagtcg cctttccttt ccatgccttc
     8521 cttccagaaa tttctgagaa ccaatggatt tgtctggtaa ttccacccac cggtgccgta
     8581 acgtgcccat tccacatggg ctcgcatcat gggttcatga tgggaggttc ctatgacaat
     8641 gccatattca tcggccaggc gcggattttc cgggtcatcg tcgtagaagg cccttcccca
     8701 catggcaggc cacagaaaat tacccctcat ccggaggatg tattcaaata cgtgttcgta
     8761 aaaccggctg ttgaacgctc cataacgttc atgcacccag cccgaaaggg ccggtgcctc
     8821 gtcgttgatg aaaatgcccc ggtaccggac tgccggttct ccgggagtat attcgcccgg
     8881 cattgcatac aggtctttag ccgggggtac tggcacatcg gcccagaaat gtaacggggt
     8941 gacacctatc tgccgggaaa ggtcatacag gccgtagatg gtgcctctct tgtcgctgcc
     9001 ggcaataacc agcgcttcgc gtatgccttc ggccggttcc tggatgactg tagcaacata
     9061 cgtctctttt tttcccttga gccacttgcc ggagatccgg ccggatcgga ccagttggtc
     9121 tatgagcccg ttctttccca gggtaccggc tatgatcatc cgctcacagg gagcagggtc
     9181 tgaacctgtg aaaaggagcg gtctgttacc ggtcactttt tccatatctg cctgtacatg
     9241 gcctgccacc cggagcaccc ccggataatc ctgaccagac acccacatgg gtgccggttc
     9301 tccttcacgg gccagggcaa atccattttc tacggggtgg cgcatgatat actcttctct
     9361 ggaacgtcct gcaagctccg ctgaccctgc caacgtcatc ataagcattg ccaagcacag
     9421 gaatttcctg tatatgccag ttgttatcat tatgtattga tttatttact atcgttcagg
     9481 ttccattgaa aggtgggggc tggcaatcct gcatcatttt gaaggttggc ttccgaggga
     9541 tcgtttgccc atgcgtacct taccccatgg ggcgtgaaat cttccttaca ccataccgcc
     9601 actttctttt tgccaataat gcgggcgctt gccctgaaat aacgtccctg gacgtcggcc
     9661 agggcaaaat gggcaggatc agggccgtct ttggttataa ggcctttgcc ggcatgagaa
     9721 aaagtgataa ccactctgtt tcccctcagt acgacatgtt gaggtagggg agacaaaggg
     9781 gccttcctgt tcccggcacc caggtattct gccgctgaaa ccaccctgtg cgccagttcc
     9841 ttcttgttga gcggatgtat atcgttccac tctcctatat cgtgcgtaac caccaatgcc
     9901 gtatgttttc ttgcccggac ggcagccagt tgttcatccc tgcggtttgc ccagtctgtt
     9961 tccataggcc agggtgcctt atctttcatg taattaggca attgcacgat tacaaaagga
    10021 agggcatcat cattccagtc ttcgcgccac cggtctatca ggatagaaag catctgaccg
    10081 taatcccttc tttttgtttc actctctccc tggtaccaga tgacaccttt tacgggcagc
    10141 tgcgtcagcg gagccagcag cgcattgtac agaccggccg gctggtagtg ccatgtggta
    10201 gtggcaggat actcagctcc ggcgttagtg agggtgcttc ccatgcgggc catccagtca
    10261 tctccctcca gggaaacggt atccgccatt gcataaaacg gatgtaccgc tccaatgtat
    10321 agcgcataat ccttgccgga agtaaaacct cccggtcccc aataattttc aatccggata
    10381 agcaattcat tgcttccggc tttgagcagg cctttgggta tggtatagat acggggagga
    10441 tactggtaca cggtagatcc cacctgggta ccattcagga acgttctgtc ggcgtctgtc
    10501 atggtgccca ggatgagcat gccatcctgg tcaggagctc cttcctgcgc atgaccgatc
    10561 tgtgtttcgg tcaggtaaaa aaccttttta aaccatgccg agccgggcga ctgcgataaa
    10621 ggtccggaag gtgttgcatc tgcctggtcc cagaaagatg gcacccggta aggaacccat
    10681 ccctgtgaac cttccggccc gggatctgga gtgaacaaag agcggtaatg tgccatggcc
    10741 tgggtctcgg cctgttttac actgtccacc cagcactcgc gcctgagggc agtgagcctt
    10801 tctgggtaat gatcggggcc cgtgggaccg ggaaatcccc tgagcgtggc ttcatccagc
    10861 caggcatcca gcggagatcc tcctacagcg ctgactatca aacccacagg aatattgtgc
    10921 ttctgattcc attccatgcc aaagaaaaaa gccagcgcag aaaattccgg gatctcttcc
    10981 tgaacgcaca gtttccacct gccagcagga atatcccaca aaggtccctg gaaattgtag
    11041 tgtgttgccg cgcggaattc acgtatctgt gtgttggaat aagtagtcgc ttctatcggg
    11101 taacggatgg ccaccctgct tacagacaat tctgtattgg attgtccgga aaacagccac
    11161 acatccccca ccaggatatc atgcaccacc cggccgttga ctttcaatgt ataagggcct
    11221 ccgggatcca tagcgggaaa agaaacactc cagttcccgc tcccgtcggc acgggtgtat
    11281 tgttctatgc cggcaaattc aacttttacc ggggcgccgg caggggcttt tccccagaca
    11341 gggatttctt taccgcgttg caggaccagt ccgtcactca gtatttttgg caaaatgacc
    11401 ggtgcgttgt aatggtcttc gtgtaataag cttaaagcag caaccggctc cttgtggggt
    11461 gtcatcctgc aggaaccggt gcatataatc aacaataagg ttatcagaac atctcttgac
    11521 atattattcg ttgggaataa acacggtatc gccttccaga cggacagtat atcgcttgcc
    11581 gcccacctcc aggacaaggt ctcccgcttc cacaacccat acctggttgt catccaggga
    11641 ggccatctgt tcgggaacta tggtgaagtc cactacacgg ctctcaccgg ctttcaggtg
    11701 gatcctttca aatccctgaa gtgattttat gggcacgggg ttggatgcat cgtgtaaaga
    11761 tacataaacc tggactactt catctccgtc cctttccccg gtgttggtca cacggaacga
    11821 tatgtctgcg gggatcccgc agggaacgct ttcgggcatc tttagggatt cgtatgtgaa
    11881 agtcgtgtaa ctgagcccat atccgaaaga atagagcggt tcgtcccgga aaaaccggta
    11941 tgtcttgcct tccatggcat attcctcaaa tgcgggaatc tggtctatgg attggtaaaa
    12001 agtgacggga agttttccgg agggattgta gtccccaaaa atcacgtcgg ctatggcatt
    12061 tcctccctcc tgtcccggat accaggcttc aatgatggcc ggcagcgatt tgacttcctt
    12121 gtcaaaactt accgcactgc cgttcattaa aaccaggacc gtgggcttgc cgagtttttt
    12181 cactgctttt atcagttcca gctgggttac gggaagcccg gtgtcaagac ggtctccctg
    12241 gttgaatcct tccaccctta ccttcatttc ttctccttca agcaacgggc ttagtcccag
    12301 gcaaaggacc accagatcac tctcagaagc cacggcaagt gcttcttcca gcaaattcct
    12361 ctgaggagct tcccaaagca gcctcatgac ggcatattct gtgttatcct gtacataacg
    12421 gagcgtaagg gcgtaaggtt tgccggcttc cagttccaca tattcatatt ctttcctggg
    12481 ttggtgttcg tcaattcttt ttatcagcat ttctccgtcc agggtgagcg tgcagccgct
    12541 gaaagcttct acccccagtg cataccgtcc cgattcgggg acacaaagta ctccggacca
    12601 ttcaacagaa taattccgga cattcatatc ctcaaaaggg gcttcgttcc accacaggaa
    12661 atcaacaacc ttgtctgtgc gctcgtgcac gggatctcct tcccaaaggg agttgtcgta
    12721 ataatgtccc gtaaggcctg gttttgccat ctgtgggtct gtgaaaagca ccccggaggg
    12781 aacaggcttg aaataaggaa ggccggcggc cagggcacat ccctgttcaa aggagacctc
    12841 ggcctgtggt aatttttccc ttataccctg cagcggggtg accggatgtt tggaaaaccc
    12901 gttgtaattg cccagaagca cttccagatc atcggcattg ggcccgatga cagccacttt
    12961 attcacctct ttggtaaagg ggaggattcc gtcgttctcc agcaggacaa ttgatttgca
    13021 ggctgcctcc cttgcaaggg aaacgtgttc aggactttcg accacttcta tgggtatttt
    13081 gctgtacggg acgttccggg gtttatcaaa cattcccagt ttgaaccggg ccagcatcag
    13141 gcgctgtaca gccgtgtcaa tgagggcttc ttctatataa cccttttcca cggcttccac
    13201 caggtaagga taggtaaccc cgcaattcag gtcagtgcct gccttgaccc ccagggcggc
    13261 cgcttctgcc ggagtttcca caatttcatg cattcctttg tcgtagaaat ccttgatggc
    13321 ccagcagtcg gaaacgacat agccctggaa gccccacctg tccctgagca aagaacccag
    13381 gaaaaagctg ccacagcaag gttctcccca aaggcgctga taggcgccca tgacggacca
    13441 aaccccgcct tcctgcacta cttttttaaa gtgtggagca tatgtttcgt aaaaatcacg
    13501 ctggttggga tggacatcgg cgctgtgcct ggtagattca ggaccactgt gaacggcaaa
    13561 gtgcttggcc gtggcaacca gtttcaggta tttgggatcg tctccctgta aacctttgat
    13621 atactgcacg gccagttcac ctgtcaggta agggtcttcc ccgtatgtct ccatgcccct
    13681 tccccatcgg gggtctctga aaatattgat gtttggtgtc cagtatgtca ggccctggta
    13741 tattccccgc ttgccctgcc gcaagaagtc gtggtgtttg gccctggctt cgtcactgac
    13801 tgccgtggct atccgaaaca tcatctcctt atcccacatg gcggccatac cgatggcctg
    13861 gggaaaaacg gtggcctgtc ctgcacgggc aaccccgtgc aggcactcat tccaccagtt
    13921 gtattccggc acacccagcc tttcgatggc agatgactcg tgggtcatct gtgatacttt
    13981 ttccttgagt gtcatccgag aaacaaggtc ctcggcccgt tcctggaatg acaggttttc
    14041 cctctggtaa gccggtttac aactccataa accggacaag agacataaaa caattaatga
    14101 tactcttttc ataaacgccg gttgttaaaa tttttccatt cagtcttgtt tgggataagc
    14161 cacaggctgt cccaactgtg ctttgccatc gaatccgagc ctgcttttgg ccgcttccct
    14221 gtctatcatg cccagcacaa cggtattcag tccttcctgt gcacagaaaa ggtacacgtt
    14281 ctggcttatg aagccggtat ccgttgcccc gtaaaaatct ttctgctgtt gatccagcct
    14341 tgtcatcctt tcgtaattgg ctacataaag cagcacaaca ggtgccttct gggcaaaagg
    14401 ctgtctcgcg atctctgccc tgaaatcccc tgtgagcagg tgcctgagga tgtgctcctg
    14461 cgggatgtac aggtaaacgg ctttgggtgt gtagacataa atgtcaatct cctgcatgtt
    14521 catggccgaa ggggccgtac gtctgccgtc gggccggttg accccggcag cggcccataa
    14581 aagatcggac aatgtctgtt gtgaaagcgg tttgtcagac atttcacggg tactgcgtct
    14641 gttgttaaaa acttccatca aaggttttcc tccttcttta ttgggtgctg gaagcgtgat
    14701 gtcctgagcg tacatgacgt taagtgataa taaaaccgtt agtgatacaa cgaatctttt
    14761 cattatgcta tttttttact ttgttacaga ctttttccca taattcatgt tcccgttctg
    14821 ttgccaggtc cagcagtgcc tgtgcttctt ccttcaacat gcgggccttt tccttatccc
    14881 tgagaccgcc aacattgatc agcacattca gaaaccctcc cttgacagcc cccagaacgg
    14941 caaatgcccc cacgccggcg tcggtaaccg aattggggtt ccctttccca atcattgcct
    15001 ccaggatgtc aaagacacgc agggatgttt tcatcgtctg caaaggaacc tgtgctgcat
    15061 acagggtggc tttttccaga tcatccttac aaggtgagga gaatgcctcc atcaccttat
    15121 tgtaggaaag cgtgtcttct tccaccaggt tgagcagctc ggcgatcagt gcctgtccct
    15181 tttcggccca ttgcgaaaaa tactcccatt ggtcgtccca gccgcgtttg tgcgaagaca
    15241 ggttggccac catcgtgccc agggaggccc ccagggctcc catataagcc gagacggagc
    15301 ctccgccggg cgcgggagac tcgctggccg tttccagggc aaattcgcgg catgtcatgc
    15361 cgcacaaccc tttttcagaa gatgtgtctt ccagcagata ttcaagaacc ctggtcttcg
    15421 gatcaaaagg cttcaactcg tccaggccca tggacttaat ggctatccgg atgatttcct
    15481 cttccggcag ccctgatgaa cgttgctgtt tgtgcagaaa atgtctgccg gcatccagca
    15541 gggtgctttt aggcaccaga cctaccaatt ccgttcctgt aaccctgatg ccgcgtttgt
    15601 atgccttgtc gcagacttcg tcaaaagcca catgaagcgg ggtgactgaa atatttgtaa
    15661 tgttcatgga cacctgggct atcccgtact ctttgatata ccatnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnaatatt tgtaatgttc atggacacct gggctatccc
    15841 gtactctttg atataccatc cgatggcctt acagtctttc aatgtccccg gttcccaggt
    15901 ttcctgacca ttgaccatta ctttcctgcc tttttccctt acatcaaagg caatggcatt
    15961 ggccctgcgg acagacgtag tgttcaggtt gaaattcacg gctatcagaa aatccctggc
    16021 ccctatcacc gatagaccag tggaggccgt tctttctgtg taccgggcgg gcccgaaatc
    16081 gggtttccac agcggatctt tcatttttgc ctccaatcct tcatattctc ctgccctgca
    16141 tactgccagg tttttccttt tttcagagaa cgcggctgat tcgtaacaat aaacgggaat
    16201 cccgagttct tcccccaccc tgcgtgccag gtccctggcg tagactacaa cctgttccat
    16261 ggtaatgccc gagacaggga tgagcggaca cacgtctgtt gcccctatgc ggggatgctc
    16321 gccgtggtgc tttcgcatgt caatgttttc agaggctgtt ttaatggctt gaaatgcagc
    16381 ttccacaacg gcttccggtg tgccggcaaa tgtcatgacc gtgcggtttg ttgcctcgcc
    16441 cggatcaaca ttaaacaggg taacaccttc taccctggaa ataacatcgg caatcctgga
    16501 aatgacctgc ttatccctcc cttcagaaaa attggggaca cattcaatga ttcgtttcat
    16561 agtgttattc gtaaatgagt ttcactttct ctccaacggc tttttccaac gccgccttat
    16621 ccagttcaaa ataatgtacc gggtacacca tgggggctcc tatctttttt acagcctgaa
    16681 cgaactgatc gtcactcatg gtatagggta aatttttggc taacagggcc acatctatgg
    16741 gtcccaggtt atccatttcg ggaatcaatt ctgtgtctcc ggaaatatat actttaacat
    16801 ccccgaaagt aaatacataa ccgttcccgt atcctttggg gtggaatggc tggccatcgg
    16861 gtcttttatt caagatgtta taggccggaa cagccaggat ttcaatgttt tcccaccggt
//
LOCUS       DAOSOF010000028        16352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3581_length_16352_cov_5.903111, whole genome shotgun sequence.
ACCESSION   DAOSOF010000028 DAOSOF010000000
VERSION     DAOSOF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_16352_cov_5.903111"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLM68_02900"
     CDS             <1..232
                     /locus_tag="PLM68_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54345.1"
                     /translation="TEMLSRAGEHVDTQEQDASPVLSGDDFITETLARIYAEQGYHKL
                     AIACYGKLILLYPEKSVYFASLVQEIKQKTNN"
     gene            236..574
                     /gene="secG"
                     /locus_tag="PLM68_02905"
     CDS             236..574
                     /gene="secG"
                     /locus_tag="PLM68_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT54346.1"
                     /translation="MYTATAILIVIASILLTLVVLVQNSKGGGLAANFATGNTTFGVR
                     QTADFLEKATWTLSIIVVVLCLLGTTFISTGKNKKASDLEQRLEQTQQPAGMPQFPTG
                     TKETPLLPTE"
     gene            complement(587..2113)
                     /locus_tag="PLM68_02910"
     CDS             complement(587..2113)
                     /locus_tag="PLM68_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT54347.1"
                     /translation="MKKVIKVSINKMPFTLAEDAYGVLKVYLDHLRRYYSGREGGSEI
                     VDGIEERIAELLAERTGMDGRVVSKEDIDNILEVMGPVEVIEEEEIDTGAGAGSYRND
                     TRPTKKLYRDVDHKILGGVCSGLAAYFNIDVIIVRVLYAVLLFIPSIVHWAVTFKSWG
                     GFIFNFPWIFVVIYVVMWAIIPAAYTVEEKYAMRGEPLSARGVQRNRPRPSGAPRYSY
                     EPRHASAERNRVLGTLGRIMAILVGLFFLVTSSAVLIGLVVAFSATGFALSFFPTALL
                     DLLAFPENMLWFKICLMVALIVPVLGFIHLGSVLVFNIKGHRWIGSTLFFIWLASVIG
                     LIITGAHGASNFRFNARFDEDVPLEHTSDTLYIDLESREDFMFDRYWLRADNSCYKLG
                     WIEGDSDDLDVVAFPSITVVRQSEIDMSQVRIKSESFGWSHAVAEEEAEDLKPVITLE
                     GNVLSVNAFQINEENPWKGNTSSLKLYVPSNQVIIVRKPIYHEFGVSRSRKVNIIRSE
                     "
     gene            complement(2110..2442)
                     /locus_tag="PLM68_02915"
     CDS             complement(2110..2442)
                     /locus_tag="PLM68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT54348.1"
                     /translation="MNENNIENIKSQMRRGVLEYCILSVLDKNDAYASELIDQLKDAR
                     IIVVEGTLYPLLTRQKNQGLLTYRWEESTQGPPRKYYSLTSKGRDLLLEMDKAWKEIV
                     ETLDILRK"
     gene            complement(2467..3078)
                     /locus_tag="PLM68_02920"
     CDS             complement(2467..3078)
                     /locus_tag="PLM68_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOT54349.1"
                     /translation="MKKVMNVGIGGSSFVIEEDAHQRLDLYLERFRSEMDSTEAGEVM
                     EDVEARIAELFYEYTKGKGEVVTLEVVERVIRQLGYSNDSRDEAESGETTRKSKRRNG
                     YTGYERYDGLGEKPRKRLYRDPDDSMVGGVCSGLAAYFNCDPVLIRVLAVATFFMGSA
                     GFWIYIILWIAVPMARTAAEKLEMRGLPVTAENLRRYGGSRRN"
     gene            3215..4411
                     /locus_tag="PLM68_02925"
     CDS             3215..4411
                     /locus_tag="PLM68_02925"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOT54350.1"
                     /translation="MESIKKIFKIGNGPSSSHTMAPRTAALDFAARNPHAAGFSVTLY
                     GSLAATGKGHFTDKALENAFHPKPVEIAWDYTTVLKQHPNGIEFRALNDSGVVLDTWL
                     TFSIGGGDLSDTGTRTSRQVIYPLNKMAHILDYCQEKGLHFWEYVAMYEEEDIWDYLE
                     HVWQTMQEAIYRGLENEGVLPGPLHLGRRAASYHIKAQGYTGSMQRRALAFSYALAVA
                     EENAAGSVVVTAPTCGSCGVIPSVMYLNKTCYNFSDKRILRALATAGLIGTLIKSNAS
                     ISGAEVGCQGEIGSACSMSAAAAVQLFGGSIAQAEYAASMGMEHFLGLTCDPVCGLVQ
                     IPCIERNAFAATRAIDANMYALLSDGTHRIHFDTVIEVMKRTGHDLPSLYKETSEGGL
                     AVVSTK"
     gene            4461..4784
                     /gene="trxA"
                     /locus_tag="PLM68_02930"
     CDS             4461..4784
                     /gene="trxA"
                     /locus_tag="PLM68_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT54351.1"
                     /translation="MIQTVTDDNFNAIVLNSTIPVLVDFWAAWCGPCRQIAPVVEEIS
                     NETEGTALICKCDVDTATEVTARYMIRSIPTLLFFKKGELVHKIVGATNKQTIEEKLK
                     SLITV"
     gene            4786..5403
                     /locus_tag="PLM68_02935"
     CDS             4786..5403
                     /locus_tag="PLM68_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOT54352.1"
                     /translation="MKKLLITIALMLSVSSAALAQGSFVVKAGLNFNKLQDIQIDNLK
                     QSWNSQTGFHAGIGGQFKIPVIGLSFQPEILYSRVRTGLLGEVGQSSYDGFRIDYIDV
                     PLNIQWGINILFMRPYIFAAPYIRYAIAKGDLLSQVDWEDLNRLDYGLSLGAGIEIWK
                     FQISGKYNWSFGQLAPGGSLHIDSNDWKLEDSNMRGFELSVAFLF"
     gene            5409..6380
                     /locus_tag="PLM68_02940"
     CDS             5409..6380
                     /locus_tag="PLM68_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT54353.1"
                     /translation="MYDLGWIRQSLGSDWKAFENILKDALDSPYSLLSSIDSYLLNKP
                     GKQLRPALALCSARICGTPGPLSHAVAAVAEMIHTASLLHDDVADNADTRRGTPSVHK
                     QYGPRASILIGDYWFARAFQTLLQYEGKHLVPLYAAVIRDLSEGEMLQLEKSRTLDIS
                     FRDYYMICKNKTASLFSASMVSGALSVTDQEVAAALRPIFEKAAYHMGMAFQIRDDMM
                     DYQPALNSGKPYYKDIAESNITLPLLGALCNAGFKDNYPSVKAWIRRLEQRESQGLDQ
                     IVDFVERYNGLEFAARVLHHHQTKAREQLSLLPPSPWQEALLKIAGM"
     gene            complement(6322..7020)
                     /gene="trmB"
                     /locus_tag="PLM68_02945"
     CDS             complement(6322..7020)
                     /gene="trmB"
                     /locus_tag="PLM68_02945"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOT54354.1"
                     /translation="MGKDKLRRFQENKSFTNLYEPRYDEVHGKDHPLKGFWAEKVFGN
                     NNPIILELGCGKGEYTLALARRDRQQNYIGIDIKGARLWRGAKTALEEKLEHVAFIRT
                     RIELIESFFAPGEVSGIWITFPDPQPRKENKRLTSPRFLDSYLSFLRPGAVIHLKTDN
                     RALHDYTREVVERRPDLALLQADADIYRNRLVPETVLEVKTFYEEMFLREGKPITYLQ
                     FSTGLPATGKGEGG"
     gene            complement(7007..7471)
                     /locus_tag="PLM68_02950"
     CDS             complement(7007..7471)
                     /locus_tag="PLM68_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54355.1"
                     /translation="MARKHHRLPFCLMAGAILTATSCSPPSSSQVQQVIPGGTWEKSH
                     ILTLTLQAPDTAGRYDSFLDIRVTPKYAHSYMSIIMIHDRRKDTLWLSLLGGNAIRSG
                     VFTDYRFPLDTAGFAADKPYWSWQLSHNMLDQALDGIATIGLLIKKHGHGKR"
     gene            complement(7452..8600)
                     /gene="ricT"
                     /locus_tag="PLM68_02955"
     CDS             complement(7452..8600)
                     /gene="ricT"
                     /locus_tag="PLM68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOT54356.1"
                     /translation="MEEKNIQHDCSRGCQVERTHMGDLQCTMDYSCSKLATFDWMEEF
                     RDVQCDNLFEVRFKNTHKGFFKNDSDIFLKIGDIVAVEATTGHDIGFISLAGPLVLKQ
                     MKRHKINPETYEFKKIYRKAKLLDLEKWQEAIAREHQVMIRSRQIAASEGLQMKIGDV
                     EFQGDGTKAIFYYIADERVDFRRLIKLFASEFGIRIEMRQFGARQEAGLIGGLGVCGQ
                     ELCCSRWMADFTSVTTQAARIQDLSLNPQKLAGQCSKLKCCIDYETSVYMDAHKEIPA
                     VNRPIETEDGPVYLVKTDVLKGVMWFSYEEDSMINMIPVHSARVKEMILANNQGKKVP
                     PLQEKPKNNGEQNGFRSAAGEESITRFDSKNKNKRKKNNRRKNGPKTS"
     gene            complement(8612..9577)
                     /locus_tag="PLM68_02960"
     CDS             complement(8612..9577)
                     /locus_tag="PLM68_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54357.1"
                     /translation="MRIDNLSHAILFSSEEGSGALPQALSLAGYILCSDKKEDGSACG
                     TCPSCRKTKNAVHPDLHFIVPVNRSAGISAARQPVTDDFLDLWRSSYLENPDITEQEW
                     YKKLEIENKQGAISAAEAGNILRKLQFRSFEGGARVIIIWLPERMHPAAANKLLKILE
                     EPVPGTYFLLVSHRPDQLLPTIRSRCMLIQVPPKEYRPLPRETYTNLERLLDLCFSGK
                     PSGVLDWAEDMATQGRESQKAFMASSMQIIRQYYMLSLGLPSLSFLDPSSLPIASRYA
                     ARMSETFFRVIYERLNTGMEQLERNVNARVLFCSLAFDFFLSLQR"
     gene            complement(9568..10521)
                     /locus_tag="PLM68_02965"
     CDS             complement(9568..10521)
                     /locus_tag="PLM68_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54358.1"
                     /translation="MKRFKIFVSTAFLIAACNVFLQAQDLNTAIEAYNLGATAAQEGN
                     YVTAIEHLDRALELGSALGEEGASVVTDCKNLIPQLYLRYAKELAANGDVASSLPFLD
                     KAAETAVKYGDQTGIQDEVKDLTSKVYYIIANEAFGKKDFAGAIESYKKVLELDPTNG
                     IAYLRLGQSAANSNMEDEAIASLQKAAEYGQADNARKLMASIYLKNALGAQKNKDWEQ
                     VYQSALKASELTDNVQSYKLLGLSAMELKRFPEALVAWENVMRADPQAKDINTTYYRL
                     AVVNESLGNKSVACSFYNQILTDPNFKSIAEYKIKTELKCE"
     gene            10631..13126
                     /locus_tag="PLM68_02970"
     CDS             10631..13126
                     /locus_tag="PLM68_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54359.1"
                     /translation="MKTRSLLIVFLFFISVVLPRAAVTAQVVPEDSRFRTGQLPNGIT
                     YYIHSNDHPGGQMLFQLVRKPDRQPFFKQILTSPQGVEEGVALLIRKLQEQLVTSPEK
                     YRPHLLAVILVGPFQADYARALVNREFSVLPASVDIMQSLEVPQGIDSAVAARVFSAR
                     PLPPVLQDGYPRIEISFPLPSLAREMRGGSEYYVMDLLRSVMEYAAARSLEYPACVYT
                     GRQFVWAVSAHPDSLAGSFHDLARQCVQLARNGITGQTLDQARESYLRQEEWHYLNRF
                     TRTNDHYAGECIRHFFDGVPMHTATWRHMFVNHLAGYVTADQVNNYIKNVLLSNPPEI
                     TLYKAVSAGDSLIRPVSFLPGELLEERDRLLTELSGMLFPFDDTLLTELAARILDIDL
                     RLQLDSLQRSHVIPVVTTDSLATLFRQVWHTTARVPARDPDKAAPVEKNGQSTGLPES
                     GKVERQMLIAHSGVSVWQLSGGSLLYVRPDTLLRGKICFAAVEREPSFFTPFIPRETY
                     RKGNGPLLWKQSPQGLLLGNATTPDSLRSFIRGAALQVNSLSVDSTALQRLWQEREKQ
                     IHAARDLSRNILLDTLKTLLFDRIPAEGPHPPKGFDFICTGDICTDSLIVLAEEFLAG
                     IPNRDIRARTPLPGEEGMRRGQHAQTISFPNPAAESKMARVYSGSCPYTLEQHVLLQL
                     LERLVSQATDNAVCVLSSLESFPGGHYFFYIGFSSHAPDFAYNEHRLEQILADLAAYG
                     PSAEQLEQARHSLLMDHLTQMADPLFHCRMLVGYCRSGKDFVTGYAGTLQQADTAMVR
                     DFVRQIMEYGNTSRVVLSGATNKTEDTSYSKNP"
     gene            13177..15711
                     /locus_tag="PLM68_02975"
     CDS             13177..15711
                     /locus_tag="PLM68_02975"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOT54360.1"
                     /translation="MPKRIVLPLLALLLSGCIRPGNDEVLVTPYTKIDFQSNGETREF
                     SISSNFLWTLEIADTWVTINPMKGYGDKQIQVTVAANTNLEPRQTTFIIAGEQTRREI
                     TVYQAAEAPALSLDSSQKTVGAAGDTVSVGVTTNLELSITPEAAWIVYNETKTITSKR
                     AIFTVQPNTTLESRSAQVEFRQKNGSLSAILNITQTGEAPGIQLQANSVQAVAAGGGY
                     KVTVVSNIEWEAKTDIPWIHITGTRLMQSRDCNFTVDANPRVGSRQGTITIYAPAYPQ
                     LGEAVVSVTQEGAEPLAVLTPSAIEDVPAAGGTYSIAVQANFNWQEDLSGTASWISKV
                     IHTANGLQVTVDRNDDVQARSTSLDITQENGSYIKGITISQVAGEQKLLLPTQANIPI
                     ATAAGGTISIPVVSNVSWKASLSEDWVSVIETKGLEAKTLTLQVAENKRIDARQARLT
                     VTTTEAGQDSLVVTRTIFQQGATPYITCDPDTLDVPAAGGAYTVPVKANVSWNVLSSP
                     SWVEGVQIKNPVEYDADLAFTVKPNQQTTARSAKIQLSRTGGSLVSDLVINQEAEAVF
                     VNATVDSPQFLDNEGDSFSLTVESNIQITYGVNVNWIINTAKTVEGRTTTWEFQVLPV
                     PTTSNRSAVLQVLETGTQQVFKTFTLTQRGARIAQKDSTALIRFHKNMRGENWRDTHL
                     WNLLLPVDTWHGVTLETAVRNGALHVKKLELPNGRLEGSVGDGTEKDPLSQLAYLEAI
                     DLSNNSGVTGWLPVSWKDLENLETINLDNCNLTNFMFFGYNIPPQYGTTLKNLTTFII
                     RNNLLNGVIPVEITKHPHFEDWNFVENMQPQKGTNRLTLPDEAGEP"
     gene            15857..>16352
                     /locus_tag="PLM68_02980"
     CDS             15857..>16352
                     /locus_tag="PLM68_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54361.1"
                     /translation="MKKFLKWSAAFLVLGVLAVGCIKNEPSPGIEAMRNAKAALLTAQ
                     AALEQAKVQVEAANAALIQAQASVIKAHEAIIAAQARKVDAETALIMAQVEWEKALTD
                     YHKQQWALNIQEREYELEVARAAADAAIKEWEVYMVQLQAQMVAATQAYEAALLQFEL
                     WKLAN"
BASE COUNT         4061 a         4329 c         4043 g         3919 t
ORIGIN      
        1 taccgaaatg cttagccggg ccggggaaca tgttgacaca caggaacagg atgcctcacc
       61 cgtattgtca ggagatgatt ttataaccga aacacttgca aggatctatg cagaacaggg
      121 ttaccacaag ctggccattg cctgctacgg gaaactaatt ttgctatatc cggaaaaaag
      181 cgtttacttt gcgtcccttg ttcaagaaat aaagcaaaaa acaaataatt agaacatgta
      241 tacagcaacc gccattctca tagtcatagc aagtattctg ctgactttgg tcgtattggt
      301 tcagaattca aaaggaggcg ggttggcagc caattttgcc actggtaata caacttttgg
      361 cgtacgtcag acagccgact ttctggaaaa agccacctgg acactatcca taatcgttgt
      421 ggtactatgc cttctcggaa ccacctttat ttccaccgga aagaacaaaa aggcttccga
      481 cctggaacag agactggagc agacacagca acctgccgga atgccccagt tccccacggg
      541 gactaaggaa acacctttac tccccacaga ataaatgaaa aaccggtcac tcggaccgga
      601 taatatttac tttccttgac cgcgacacgc caaactcatg atatatgggt ttgcgtacta
      661 ttatcacctg gttcgaaggc acatagagtt tcaatgagct ggtgttgcct ttccacggat
      721 tttcttcatt tatttggaaa gcattaacag acaggacatt gccttccagg gtgatgacgg
      781 gcttcagatc ttccgcctcc tcttcggcaa ccgcatgaga ccaaccaaaa gactcggatt
      841 ttatgcgaac ctgggacata tcaatttcag actgcctgac aacggtaatg gacgggaaag
      901 ccaccacatc caggtcatca ctatcccctt cgatccaccc gagcttgtaa caggagttgt
      961 cggccctgag ccaataccgg tcaaacatga aatcttccct cgattccagg tcaatataga
     1021 gggtgtctga agtgtgttcc agtggcacgt cctcatcaaa acgtgcattg aagcggaagt
     1081 tcgaggcacc atgagcccct gtgatgatga gtcctattac cgaggccagc cagatgaaaa
     1141 acaacgtgga cccgatccac cggtgaccct ttatgttgaa gacaaggaca gaccccaggt
     1201 ggatgaaacc cagcacggga acaatcagag ccaccatgag gcatatcttg aaccaaagca
     1261 tgttctcggg gaatgccagc aaatccagca gtgccgtagg gaagaagctc aaagcaaagc
     1321 ccgttgccga aaaagctaca accaggccaa tcagcacagc agaactggtg accaggaaaa
     1381 acaaacccac caggatggcc ataatacggc ccagggtacc caggaccctg tttctctctg
     1441 cagaagcgtg cctcggttca tatgaataac gcggggctcc cgagggtctg ggccggttac
     1501 gctgcacccc ccttgcagag agcggttctc cgcgcatggc atatttctct tccaccgtat
     1561 atgcagccgg gatgattgcc cacatgacca cataaataac cacaaagatc cagggaaaat
     1621 taaaaatgaa accaccccag ctcttgaacg tgactgccca gtggacgatg gagggaatga
     1681 acagcaaaac ggcatacagc acccggacaa tgatgacatc tatattgaaa taggcggcaa
     1741 gtcccgagca gacaccaccc aggatcttat gatccacatc ccggtacagt tttttagtcg
     1801 gccttgtatc gtttctgtaa gaacctgctc ccgctcccgt gtctatctcc tcttcttcaa
     1861 tgacctctac aggacccatg acctccagga tattgtcaat atcttcctta gataccaccc
     1921 ggccgtccat gccggtgcgt tctgccagta attcggctat gcgttcctca atgccatcca
     1981 caatttccga tccgccttcc ctgccggaat aatatcggcg caggtggtcc aggtaaactt
     2041 tcagcacacc ataggcatcc tctgccaggg taaacggcat tttattaatg cttactttga
     2101 ttactttttt cattttctca ggatatcaag tgtttccaca atttctttcc atgctttgtc
     2161 catttccagc aacaagtccc ttcccttgct ggtaagcgaa tagtatttcc tgggagggcc
     2221 ctgggtggat tcttcccagc ggtaagtcaa caatccctgg tttttctgac gggtcagcag
     2281 gggatacagt gtcccttcca ccacaatgat ccgcgcatct ttcaactggt ctatcagttc
     2341 agatgcatag gcatcattct tgtccagcac gctcaggatg caatattcca gcacacccct
     2401 gcgcatctgc gactttatgt tttcaatgtt attttcgttc ataacggcaa tcattaatga
     2461 caattactaa ttcctacggc ttccgccata ccggcgcagg ttttctgccg taacgggaag
     2521 tcccctcatt tcaagctttt cagcagcagt gcgtgccatg ggaaccgcaa tccataaaat
     2581 aatgtagatc cagaatcctg cgctgcccat gaagaaggtg gcaacagcaa ggacacgtat
     2641 cagtaccgga tcacaattga agtaggccgc caggccactg cagacacctc cgaccatgga
     2701 atcatcgggg tccctgtaca ggcgtttgcg gggcttttca cccagaccgt cgtagcgttc
     2761 gtaacccgtg tagccgttgc gcctttttga tttgcgcgtt gtttcccccg attctgcctc
     2821 gtccctggaa tcatttgagt agcccagttg ccggatcaca cgttccacga cctcaagagt
     2881 gaccacctca cctttaccct ttgtatattc gtagaaaagt tccgcaatcc gggcttcaac
     2941 atcttccatg acttcaccgg cttcagtgct gtccatttcg ctgcggaagc gttccaggta
     3001 gaggtccagt ctttgatggg catcttcctc aatgacaaag ctgcttccgc caattccaac
     3061 attcatgact tttttcataa ttttaaccaa atattagggt gttatcagat tattacgccg
     3121 caaagatata taaaaaaatt gataccttgt accgcaaggt actaaaatta ttaaaaaaag
     3181 gacaagaaat gcagatagtg ctatctttgt aacaatggaa agcattaaaa aaatattcaa
     3241 gattggtaac ggaccttcca gttcccacac catggccccg cgtaccgcag ccctggattt
     3301 tgcggcccgg aacccacatg cagctggctt ttccgttacc ctttacggca gccttgcagc
     3361 taccggcaaa ggccacttta ccgacaaggc gcttgaaaat gctttccatc caaaaccggt
     3421 agaaatagcc tgggactata ccactgtcct gaaacagcat cccaacggga ttgaattcag
     3481 ggcactgaac gacagtggcg tggtactgga cacatggctc accttcagca taggcggcgg
     3541 tgacctgtcc gataccggaa caagaacgtc acgtcaggtc atatatccgt tgaacaagat
     3601 ggcccatatc ctggattatt gccaggaaaa gggtctccac ttctgggaat atgtggccat
     3661 gtacgaggaa gaagacatct gggattacct ggaacacgtg tggcaaacca tgcaagaggc
     3721 catttaccgg gggcttgaga atgaaggggt gcttccgggg ccgctccacc ttggccgccg
     3781 tgcggcatcc tatcatatca aggcgcaggg atacacaggc tccatgcaac gcagggcact
     3841 ggctttttcc tatgcccttg ccgtggccga ggaaaacgca gccggaagtg ttgtggtcac
     3901 tgcccccacc tgcggcagct gcggggtcat cccctctgtg atgtatctta acaagacctg
     3961 ctacaatttt tcagataaaa gaatattgcg tgcccttgcc accgccggtc tcattgggac
     4021 acttataaaa tccaatgcat ccatttcggg ggccgaggta gggtgccagg gggaaatagg
     4081 atctgcctgt tccatgtcgg cagcagctgc cgtgcagctc ttcggaggca gcatagccca
     4141 ggccgagtat gcagccagca tggggatgga acatttcctg ggactgacct gtgatccggt
     4201 gtgcggcctg gtgcagatcc cctgcataga aagaaacgcc tttgctgcca ccagggccat
     4261 tgacgccaat atgtacgcat tgctttccga cgggacccac cgcatacatt ttgatacggt
     4321 catagaggtg atgaaacgca ccggccatga tctgccatct ttatacaaag agacatcgga
     4381 aggaggcctt gctgttgtgt caaccaaatg atttttctta attttacagt aaatatttca
     4441 gaattaatca acataatact atgattcaga cagtaacaga tgacaatttc aatgcgatag
     4501 ttttgaactc cacaatcccc gttctggttg atttttgggc agcctggtgc ggtccctgcc
     4561 gtcagatcgc ccccgttgta gaagaaatat ccaatgaaac ggaaggcaca gccctgatat
     4621 gcaaatgcga cgtggacacg gcaacggaag tcacggcacg gtacatgatc cgcagcatac
     4681 ccaccctgct gttcttcaaa aaaggagaac tggtgcacaa aattgtggga gcgacaaaca
     4741 aacagaccat agaagaaaaa ttaaaatcct taatcactgt atagtatgaa aaaactcctg
     4801 attacgatcg ccctaatgct tagcgtaagt tctgctgccc tggcacaggg tagtttcgtt
     4861 gttaaagcag ggctcaattt caacaaacta caggatatcc agatagacaa tctgaaacag
     4921 tcctggaatt ctcagaccgg tttccacgca gggataggcg gacaattcaa aattcccgtc
     4981 atcgggcttt ctttccagcc ggaaatcctt tacagccgtg tgcggacagg cctgttggga
     5041 gaagtgggtc aaagctccta tgacggattc agaatagact atattgacgt tcccctgaat
     5101 atccaatggg gtatcaacat ccttttcatg agaccttata tctttgcagc tccttatata
     5161 aggtatgcca ttgccaaggg agacctgctg agccaggtgg actgggaaga cctgaaccgt
     5221 ctggactacg ggctgagcct tggagccggc attgagatct ggaaattcca gatatcgggc
     5281 aaatacaatt ggagcttcgg tcagttggcc ccgggaggat cacttcacat tgattccaat
     5341 gactggaaac tggaagattc caacatgagg ggatttgaac tttcagttgc tttcctcttc
     5401 tgaaaggaat gtatgatctt ggctggatca ggcagtcttt gggctccgac tggaaagcct
     5461 ttgaaaatat cctgaaagat gcccttgatt caccctattc tctgctttcc agtatagaca
     5521 gctacctgct gaacaagccc ggcaagcaac ttaggccggc gctggccttg tgcagcgcca
     5581 ggatatgcgg caccccgggc cccctgagcc atgcggtagc cgccgttgcc gaaatgatcc
     5641 ataccgcatc gctccttcac gacgatgtgg cagacaacgc cgacacacgc agggggactc
     5701 ccagcgtgca taagcaatac ggacccaggg cgtccattct cataggagat tattggttcg
     5761 cccgcgcttt ccaaaccctg ctgcagtacg aggggaagca cctggttccc ctgtacgccg
     5821 cggtcatccg ggacttaagc gagggggaaa tgcttcaact ggaaaaatca cgcacactgg
     5881 atatttcctt cagggattat tatatgatat gtaaaaacaa gaccgcatcg ctattcagcg
     5941 cttccatggt gtcaggggcc ctgtccgtaa cggatcagga ggttgctgct gccttgcgtc
     6001 ccattttcga aaaagcggcc taccacatgg gtatggcttt ccagatccgg gacgatatga
     6061 tggattatca gccggcgttg aattccggga aaccctatta taaggatatt gccgaatcca
     6121 atatcacgct gcccctgctc ggggcattat gcaacgccgg ttttaaagat aattacccct
     6181 ctgtcaaagc ctggatccgc cggctggaac agcgggaaag ccagggattg gaccagattg
     6241 tggactttgt ggaaagatac aatggcctgg aatttgccgc cagagtgttg catcatcatc
     6301 agacgaaggc gcgggagcag ctcagcctcc ttcccccttc cccgtggcag gaagccctgt
     6361 tgaaaattgc aggtatgtga tgggctttcc ttcccgcagg aacatctctt cgtaaaatgt
     6421 tttcacttcc agcaccgttt ccgggaccag cctgttgcgg tatatatcgg cgtctgcctg
     6481 cagcaaggca agatcaggcc gcctttcaac cacttctctt gtgtagtcgt gcaaggcacg
     6541 gttatctgtt ttcaggtgga tgactgctcc cggcctgagg aatgacaggt aactatccag
     6601 gaaacgaggc gaagtcagcc gcttgttttc ttttcttggc tgagggtccg ggaaggtgat
     6661 ccagatccct gaaacctctc ccggggcaaa gaagctctct atcaactcta tcctcgtacg
     6721 aatgaatgcc acatgctcca gtttttcctc cagggctgtt ttggcccccc gccacagcct
     6781 ggcccccttg atatcaatgc cgatatagtt ttgctgccgg tcccgcctgg ccagggccag
     6841 ggtatattcc cccttgccgc atcccagttc caggataatg ggattgttgt tgccaaagac
     6901 tttctccgcc cagaaaccct tcaggggatg gtcttttccg tgaacctcat cataacgggg
     6961 ctcatacagg ttggtaaagc ttttattttc ctggaaccgg cgtagtttat cttttcccat
     7021 gcccgtgttt ctttatgaga agaccaatgg tggcaatgcc gtccagggcc tgatccagca
     7081 tgttgtggga taactgccat gaccaataag gtttatccgc agcaaatccc gcagtgtcca
     7141 gcggaaagcg ataatccgtg aaaactccgg aacggattgc attgccgcca agaagggaca
     7201 accagagggt gtcttttctc ctgtcatgga tcatgatgat actcatatag gaatgtgcgt
     7261 actttggtgt caccctgata tccagaaagg agtcgtaccg acctgctgtg tcgggtgcct
     7321 ggagtgtcag cgtcaggata tggctctttt cccacgttcc gccaggaata acctgctgta
     7381 cctggcttga ggatggggga gagcaggatg tggcagtcag gatggccccg gccatcaggc
     7441 aaaagggcaa cctatgatgt tttcgggcca tttttcctgc ggttgttttt cttccgctta
     7501 ttcttgttct tgctgtcaaa acgcgtgatg ctttcttctc ctgcagcgga ccggaatccg
     7561 ttctgttccc cgttgttctt cggcttttcc tgcagggggg gcactttttt accctggttg
     7621 tttgccagga tcatttcctt tacccgtgct gaatggaccg gtatcatgtt gatcatgctg
     7681 tcctcttcat aactgaacca catgacgcct ttgagcacat ccgttttgac caggtatacc
     7741 ggaccatctt ctgtttctat ggggcggtta acggcaggga tttccttgtg ggcatccatg
     7801 taaacagacg tttcgtaatc tatgcagcat ttgagcttgg aacactggcc cgccagtttc
     7861 tgcggattga gcgacaagtc ctgtatcctg gccgcctggg ttgttacaga agtgaaatcg
     7921 gccatccacc ggctgcagca gagttcctgc ccgcaaacgc ccagaccacc tatcaaaccg
     7981 gcttcctgcc ttgccccgaa ctgtctcatt tcaatgcgta tgccgaattc agaggcaaag
     8041 agtttgatca ggcgcctgaa atcaacccgt tcatcggcta tgtagtaaaa aatggccttg
     8101 gtcccgtccc cctggaactc cacatctcca attttcatct gcaggccttc actggccgct
     8161 atctgccggg aacggatcat cacctggtgc tctctggcaa tggcttcctg ccatttttcc
     8221 aggtccagca acttggcttt ccggtagatt ttcttgaatt cgtaggtctc cgggttgatt
     8281 ttatggcgtt tcatctgttt gagcacaagc ggcccggcca ggctgataaa tcctatatcg
     8341 tggccggtag tcgcttctac ggcaacaata tccccgattt tcaggaatat gtcggagtca
     8401 tttttgaaga aacccttatg tgtgtttttg aaacggacct cgaacaggtt atcgcattgt
     8461 acatcccgga actcttccat ccagtcgaaa gttgccagtt tgctgcagct gtagtccatg
     8521 gtgcactgca gatcccccat gtgagttctt tccacctggc aaccgcgtga gcaatcgtgt
     8581 tgtatgtttt tttcttccat gaattgtcag actatctttg caaagatagg aaaaagtcaa
     8641 aagccaggga gcagaacagc acccgggcgt tcacgttgcg ttccagctgt tccattcctg
     8701 tattcaggcg ttcatagatc acccggaaaa aagtttcaga catcctggct gcatacctcg
     8761 aggcaatagg gagggaggat gggtccagga aagacagcga tggcaggccc agtgacagca
     8821 tgtaatattg ccggatgatc tgcatggagg aagccatgaa cgctttttgg gactctctcc
     8881 cctgcgtggc catgtcttcg gcccagtcca ggacacccga aggctttcct gaaaaacaca
     8941 ggtccagcag tctttccagg ttggtatagg tctcccgggg cagaggcctg tattctttag
     9001 gtggcacctg aatgagcatg caacggctcc tgatggtggg cagcagctgg tccgggcggt
     9061 ggcttaccag gagaaaatac gtcccgggaa caggctcttc caggatcttc agcagtttgt
     9121 tggctgcggc aggatgcatg cgttcaggga gccatatgat gattacccgt gccccgccct
     9181 caaaagaacg gaactggagt ttcctgagta tatttcctgc ctcggccgca cttattgcac
     9241 cctgtttgtt ttcaatttcc agttttttat accactcctg ttctgtaatg tccggattct
     9301 ccaggtaaga tgagcgccac aggtccagga aatcgtcggt cacaggctgg cgcgcagcag
     9361 atatgccggc agaacggttt acgggaacaa taaaatgcag gtcaggatgt acggcgtttt
     9421 tggttttacg gcaagatgga cacgtcccgc aggcagagcc gtcttctttc ttatcggaac
     9481 aaaggatgta tccagccaga gagagggcct gcggtaatgc acctgagccc tcttccgaac
     9541 tgaataatat ggcgtgagac aaattgtcta ttcgcacttc aattccgttt tgatcttata
     9601 ttctgcaatg gatttaaagt tgggatcggt caggatctgg ttgtaaaagc tgcaggcaac
     9661 ggatttattt cccagggatt cgtttaccac ggccaggcgg tagtatgtgg tgttgatgtc
     9721 tttggcctga ggatctgcgc gcattacatt ttcccaggca acgagagctt ccggaaaacg
     9781 tttgagctcc atggctgaca ggcccagcaa tttgtaggac tgtacgttgt ctgtaagttc
     9841 agaagctttt aaggctgact ggtaaacctg ttcccaatct ttgtttttct gtgcacccaa
     9901 agcgtttttc aggtatattg atgccatcag cttgcgtgca ttgtctgcct gcccgtattc
     9961 agcggctttc tgcagggaag caatggcttc gtcttccatg ttgctgttgg cagccgactg
    10021 tcccaggcgc agataggcta tgccattggt cggatccagc tccagcactt ttttataact
    10081 ctctattgcg ccggcaaaat ccttttttcc gaaagcctcg ttggctatga tgtaataaac
    10141 ttttgatgta aggtctttga cctcatcctg aattccggtc tggtcaccat atttgacagc
    10201 cgtttctgct gctttgtcaa gaaaaggcag ggaggaggcc acatctccgt tggctgccag
    10261 ctctttggca taacgaagat aaagctgtgg aataaggttt ttacaatcag taactacaga
    10321 tgcgccttct tctcccaggg cgcttcccag ttccagggca cggtccaggt gttcaatggc
    10381 agtgacgtaa ttaccttcct gtgcagcagt tgctcccagg ttatatgcct cgatggcagt
    10441 attgaggtcc tgtgcctgca gaaaaacgtt acaggcggca atcagaaagg cggtagagac
    10501 aaaaatcttg aatcgtttca ttagttcctt aatatatagg gggttaaaaa atagcaattt
    10561 ctcaaacaaa caaacaaagg tagcgctttt ttaatacctt tgcaattgaa tatcacataa
    10621 atcgtgccgt atgaaaacgc gcagcttatt gatagttttt ctttttttca tatccgttgt
    10681 cttaccacgg gctgccgtca cagcgcaggt tgtgccagaa gacagccgtt ttcgcacagg
    10741 ccagctaccc aacgggatca cctattatat acattccaat gaccatcccg gtggacagat
    10801 gctttttcag ctggtccgga aaccggacag gcaacctttt ttcaaacaga tccttacctc
    10861 accccagggt gtggaagaag gagtggccct gcttatcagg aaattacagg agcaactggt
    10921 gacgtctccg gaaaaataca gaccgcatct gcttgcggtt atcctggtgg gacctttcca
    10981 ggcggactat gcccgtgcgc tagtcaacag agagttctcc gtacttccgg cttctgtgga
    11041 tatcatgcag tccctggagg ttccacaagg cattgactcg gctgtggccg cccgtgtatt
    11101 ttcggcaagg cccctgcctc ccgtcctcca ggacggttat ccccgcatag aaatctcgtt
    11161 cccgctgccc tctcttgcca gggaaatgcg tggcgggagc gaatattatg tcatggatct
    11221 cctgcgcagt gtgatggaat acgcggcggc acggtcgctg gaatatcctg cctgtgttta
    11281 taccggcaga cagtttgtgt gggcagtgag tgcacacccc gacagcctgg cagggtcttt
    11341 ccatgatctt gcacggcaat gcgtgcagct ggcccgtaac ggcattaccg gacagacact
    11401 ggatcaggcc cgggaatcat acctaagaca agaggaatgg cattacctga acaggttcac
    11461 gcgcacgaac gaccactatg caggagagtg tatccgtcat ttttttgacg gggttcccat
    11521 gcataccgca acctggcgtc acatgttcgt gaaccatctg gcaggatatg tcacggcaga
    11581 ccaggtgaac aattatatta aaaacgtact gctttccaat cctcctgaaa tcacactata
    11641 caaggcggtt tcggccggtg acagccttat ccgcccggta agcttcctgc ccggtgagct
    11701 tcttgaggaa agagaccggc tgcttaccga gctttccgga atgttgttcc ccttcgatga
    11761 cacccttctg accgaacttg cagccaggat attggacata gacctgcggt tgcaactgga
    11821 cagcctgcag cggagccatg ttattccggt tgtcaccact gacagcctgg caaccctgtt
    11881 ccggcaggta tggcatacta cagcccgggt tcccgcaagg gatccggata aagctgcccc
    11941 ggtggaaaag aacgggcaat ctaccggatt gccggaaagt ggaaaagtgg aacggcagat
    12001 gctaattgcc cacagcgggg tatctgtctg gcaactgtcg ggagggtccc tgctatatgt
    12061 aaggccggac acccttctgc ggggaaaaat ctgttttgcc gctgtagaaa gggaaccgtc
    12121 ctttttcaca cccttcatac cccgggaaac atatcgcaaa ggcaacggcc cgctcctgtg
    12181 gaaacaatcc ccgcagggac tccttctcgg caatgccacg acaccggatt cgctgcgaag
    12241 cttcataaga ggagccgctt tgcaggtgaa cagcctgtcg gttgattcaa cagccttaca
    12301 aagactttgg caggaaaggg aaaaacaaat ccatgcagcc cgtgacctgt cacggaatat
    12361 cctgctggac accctgaaga cattgctctt tgacaggata cccgcggaag gtccgcatcc
    12421 tcccaaaggc tttgatttca tttgtaccgg agatatctgc acggattccc tgatagtcct
    12481 ggcagaagaa ttcctggcag gcattcccaa ccgggacata cgcgccagga caccgctccc
    12541 gggggaagaa ggcatgcgca ggggccagca cgcacaaacc atctctttcc ccaaccctgc
    12601 cgctgaaagc aaaatggccc gggtttattc cggttcatgt ccctataccc tggagcagca
    12661 tgtactgctt cagttactgg aaagactggt atcacaggcc acagacaatg cagtctgcgt
    12721 gctctcatcc ctggagtctt tccctggcgg ccactacttc ttctatatcg ggttcagctc
    12781 ccatgcccct gattttgcct acaacgaaca ccgcctggaa cagattctgg ccgacctggc
    12841 ggcttacgga ccttctgccg aacagctgga acaggcacgt cattctttac ttatggatca
    12901 cttgacacag atggccgacc ccctgttcca ctgcaggatg ctggtcgggt actgccggag
    12961 cggaaaggac tttgtcaccg gctatgccgg cacgctgcaa caggcagaca cagccatggt
    13021 cagggacttt gtaaggcaaa tcatggagta cggaaacact tcccgggtgg tgctttccgg
    13081 tgcaacaaat aaaacggaag atacgtctta ttcaaaaaat ccgtaacttg caccccctta
    13141 cgggattttt tccaaaaatt gaaacttatg tcaagtatgc caaaacggat agttctgccc
    13201 ctgctggccc tgcttttgag cggatgcatc aggcccggaa acgacgaagt gcttgtcact
    13261 ccctatacaa aaattgattt tcaatccaac ggggaaaccc gggaattttc catatcctcc
    13321 aattttctct ggacccttga aattgccgat acctgggtta ccatcaaccc catgaaaggt
    13381 tatggcgaca agcagatcca ggtaacggtc gctgcgaaca ccaatcttga gccccgtcag
    13441 acaaccttta tcattgccgg ggaacagacc cgcagggaaa taacggttta ccaggccgcg
    13501 gaagctcccg cgctgagcct ggacagctcg caaaaaacgg taggcgcagc aggtgacacg
    13561 gtatctgtag gcgttacaac caacctggaa ctgagcatca cacccgaagc agcctggatt
    13621 gtatataacg aaacaaagac cataacctcc aaacgtgcca tcttcaccgt tcagccgaac
    13681 acaacgctgg aaagccgctc ggcgcaggtg gaattcagac agaagaacgg atccctctcg
    13741 gccattctca acattaccca gacgggtgaa gcccccggga tacaactcca ggccaattcg
    13801 gttcaagccg ttgccgccgg cggggggtac aaggtcacgg tggtttccaa catagaatgg
    13861 gaagccaaaa cggatatacc gtggatccat attaccggca cacgcctgat gcagtcccgc
    13921 gattgcaatt tcacggtaga cgcgaatccc cgtgtgggat cccgccaggg gacaataacc
    13981 atatatgctc ccgcataccc gcaactgggc gaggctgttg tttcagtcac ccaggaaggg
    14041 gctgaaccgc tggccgtgct cacgccctcc gccattgagg acgttccggc agcaggcggg
    14101 acctacagca tagcggtgca ggccaatttc aactggcagg aagacctttc ggggacggca
    14161 tcgtggattt cgaaggtgat acacacagcc aacgggctgc aagtcaccgt agacaggaac
    14221 gacgatgtgc aggccaggtc gacatcgctt gacataacac aggaaaacgg ttcatatata
    14281 aaaggtatca ccataagcca ggtggccggt gagcagaaat tgttactgcc gacccaggca
    14341 aacataccta tagccactgc tgccggtggc acaatctcca ttccggtggt atctaacgtt
    14401 tcctggaaag cttccctgtc cgaagactgg gtctccgtaa tagagaccaa aggcctggag
    14461 gcaaaaacgc tgacacttca ggtggctgaa aataagcgta ttgatgcacg ccaggctcgg
    14521 cttacggtta ccacgaccga agcgggacag gattccctgg tggtcacccg aaccatattc
    14581 cagcagggag caacacctta tatcacctgt gaccccgata ccctggacgt cccggctgcc
    14641 ggtggcgcct acactgttcc ggtgaaggcc aatgtgtcct ggaatgtgct ttcctcccca
    14701 tcctgggtgg aaggcgtgca aataaagaac cctgtggaat atgacgcaga cctggctttt
    14761 accgtgaagc ccaaccagca gaccacggcc cgttctgcga aaatccagct ttctcgcacc
    14821 ggagggagcc ttgtctcgga cctggtgata aaccaggagg ccgaggctgt ttttgtcaac
    14881 gcgacggtgg attccccgca attcctggac aacgaagggg atagcttctc cctgaccgtg
    14941 gaatccaata tccagataac atatggggtg aatgtgaact ggatcatcaa taccgcaaag
    15001 actgtggaag gcaggacaac gacctgggaa ttccaggtgt tgccggttcc cacgaccagt
    15061 aaccgaagcg cggtgttgca ggtccttgaa acagggaccc agcaggtatt caaaaccttt
    15121 accctgaccc agcgcggagc ccgcattgca caaaaggaca gcacggccct gatccgtttc
    15181 cacaagaaca tgcgcggtga aaactggagg gacacccatt tgtggaactt gctgctgccg
    15241 gtggatacct ggcacggtgt aaccctggaa accgctgtaa ggaacggggc tctgcatgtt
    15301 aaaaaactgg agcttcccaa tggccgcctg gaaggatctg tgggagacgg gaccgagaaa
    15361 gacccgcttt cccagctggc atacctggaa gccattgacc tatccaacaa ctccggggtc
    15421 accgggtggc tgcctgtttc ctggaaggac ctggagaacc tggaaaccat aaaccttgac
    15481 aattgtaatc tgaccaactt catgtttttc ggctacaata ttcccccgca atacgggaca
    15541 accctgaaga acctgacaac cttcatcatc cgcaacaacc tgctcaacgg cgtgatacct
    15601 gtggaaatca caaaacatcc gcattttgag gattggaatt ttgtagaaaa catgcagccc
    15661 cagaaaggga ccaaccggct taccctgccg gacgaagccg gggaaccata aaaaccgggg
    15721 gtttccaaca agttttcaac aaaaaaagtt ttgtggattc aaattatggt ttatctttgc
    15781 acttatgtat atcatagcat aaaagtaata tctgataact tctaaaaact tttgtttaac
    15841 ttaaaatttt taatgcatga aaaaattttt aaaatggagt gcagcctttt tagtactggg
    15901 tgtactcgcc gtaggctgta ttaagaatga gcccagccca ggcattgaag ccatgcgtaa
    15961 tgcaaaggcc gccctgctga cagctcaggc cgctctggaa caggccaagg tacaggtaga
    16021 agctgccaat gccgctttga tccaggctca ggcaagtgtc ataaaggctc atgaagccat
    16081 catagccgcc caggccagga aagttgacgc agaaacagct ctcatcatgg cacaggttga
    16141 atgggaaaag gccttgactg attaccataa acaacaatgg gccctgaata ttcaagaacg
    16201 ggaatatgaa cttgaagtgg cacgtgcagc agccgatgcc gccatcaagg aatgggaagt
    16261 ctatatggta cagctgcaag cccaaatggt agcagccacc caggcttatg aagcagccct
    16321 tcttcaattt gaactgtgga agcttgccaa tg
//
LOCUS       DAOSOF010000029        16121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3649_length_16121_cov_5.321424, whole genome shotgun sequence.
ACCESSION   DAOSOF010000029 DAOSOF010000000
VERSION     DAOSOF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_16121_cov_5.321424"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..975)
                     /locus_tag="PLM68_02985"
     CDS             complement(10..975)
                     /locus_tag="PLM68_02985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT54362.1"
                     /translation="MAGKRKYKYNPKTLSYELQKNSLRFYLIRVFVLLPISLVVALGY
                     YLVYTEGFKLKTPRLVKLEREYENAQSGLELLNKQMTQAEKRLENLQLRDNNIYRNIF
                     GMEQIPGEVRNAGFGGSNRYPHLEYVQNSKLLLTTALSYDRIMKKAYIQSKSFDDVER
                     VAGQVGEVAKCIPSILPIPEDTRNFRWSGGFGYRKDPVYGDIRMHEGIDLTGAPNSPV
                     YATGDGKVEAIRFEYNGYGRYLLIDHGFGYKTRYAHLNKTTVYEGQLVKRGEQIGLLG
                     ATGKSTGPHIHYEVIYMGRPVNPYNYFDNNIATDDYHAVILGGRQ"
     gene            1057..3675
                     /gene="alaS"
                     /locus_tag="PLM68_02990"
     CDS             1057..3675
                     /gene="alaS"
                     /locus_tag="PLM68_02990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT54363.1"
                     /translation="MMTSGDIRKTFLNFFEKKGHTIVPSAPMVIKNDPTLMFTNAGMN
                     QFKDWFLGNEPAAHRRVANTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKESITWAWELLHQVFKLDADRLYATVFEGSDEDSLGPDDESRAIWEEFLPPERILAG
                     SKKDNFWEMGETGPCGPCSEIHVDLRSPGERAEVKGVSLVNKGHPLVVEIWNLVFIQF
                     NRKTDGKLVALEQKHVDTGMGLERLCMAIQGKTSNYDTDLFQGLIRSIGLLCNKEYGK
                     DDQVDVAMRVISDHLRAICFSIADGQLPSNVKAGYVIRRILRRAVRYGHSFLGFDKPF
                     LCELVPSLIQQMGEAFPELIQQQGLIQNIIRQEEEAFLRALEKGTRLFEYFVRDKTLK
                     EFSGKDAFLLYDTYGFPLDLTILMAREKGLAVNIAEFEEELQAQKDRARKATLVKEGD
                     WVEVRPFIQPSFLGYETTETFSRITRYRKVRSKNREFYHIVLDQTPFYAQSGGQVGDS
                     GYLENTQGEKIPVTDTIKENNLILHISPNAPENPETEFRAVVDRERRQTIANNHTATH
                     LLHYALRQVLGPQVEQKGSQVSAEGLRFDFSHYARMEPEEIRKTERIVNDLIRKNIVQ
                     DALQDIPLEEAGQMGAIALFGEKYGAKVRVIRFGESIELCGGTHTSATGNIGIFKIAS
                     EGAIAAGVRRIEAITGLAVERKIEEMETMLLGVKNLMNDHPDIIASVQKMLHENEDLH
                     KVVEENRRERAVSLGKSLWEQRSSLNGIQFVHLRGVYMPEMIKDMAFSFREGNYDNMA
                     FAAAYEHNGKANLTLLLSKDLVSQGLHAAEIVRNAARHIGGGGGGQDFYATAGGKDTG
                     GLQAALDAIAEALKNH"
     gene            3687..5144
                     /locus_tag="PLM68_02995"
     CDS             3687..5144
                     /locus_tag="PLM68_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT54364.1"
                     /translation="MKIFDRFLVRSFLGPLFLTIFIVLFVLVLQFLWVYIDELVGKGL
                     GLKVILEFLFWGTCTVIPMALPLATLLASIMTMGNLGEFNELLAMKAAGISLQRIMRP
                     LIILSFLVSVVAFFSANNLMPLAYKNIFALRDDISRTKEEIRIPTGIFYNGIDNYSLR
                     VEHRDKATDMLYDVMVYNHSSRRGNSSVTLADSGSIRLTEDKKNILFTLFSGETYEEG
                     ERKSVADTSFAMQRIKFEKQEIVIELTNYAFQRRDGDRFGSDVMAQGVRNLGHRRDSL
                     DSLYTISLDLFQKRMLIAGGLAFARQMDTSDRQQYKGDFPIEQLPDPSKSTQYPDLLN
                     RAIGKVDQMINQVDNYMIEEVQIHLPLRRTKVEWYRKFTVAFACFIFFFIGAPLGAII
                     RKGGLGTPVIVSMLFFVVYYVIDISGKKLATDGSVTAAMGTLISSLVLLPLGVFLTYK
                     ATTDSALFNKDAYLVMIRKIRKHLPLRHDKENEQA"
     gene            5119..6087
                     /gene="fmt"
                     /locus_tag="PLM68_03000"
     CDS             5119..6087
                     /gene="fmt"
                     /locus_tag="PLM68_03000"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT54365.1"
                     /translation="MIKKTNKPDIVFMGTPGFAAGVLLELLRQGYPVKGVVTAPDKPS
                     GRGLVMRPSAVKMTALEHNLPVLQPVSLKDEGFLESLALWDAPVFVVVAFRMLPPQVW
                     QMPSHGCLNLHASLLPMYRGAAPINHALINGEKETGLTTFLIDEQIDTGAILLQHPIP
                     VEERDNAGSLHDKMMEAGGPLVARTIDGLWSGSLKPVPQPFVAPEEVKPAPRISRETC
                     RIDWNREPGQIVNLVRGLSPYPCAFSTLKKGDTLMDIKVYEARAEIVLHGDSPGEVVS
                     DWKTTIKIACCGGYVHLLEVQAPGKKRLAAGAFLAGFRNTEELTFL"
     gene            6084..6767
                     /locus_tag="PLM68_03005"
     CDS             6084..6767
                     /locus_tag="PLM68_03005"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOT54366.1"
                     /translation="MKMKEAVILAQGAYPSHPYPLHLLETAGTLICTDGAVNKLQGRE
                     PDRVVGDLDSLSETLREKYSSRLVQDISQETNDLTKAVQYCFREGFRSIVILGATGLR
                     EDHTLGNISLLGTYAQMMDTVRMVTDHGVFFVFARGTQKADGFMEVNIPCPAGNPVSF
                     FAINPAMRLRAKGVHYPVESVVFDSWWKATLNRCTGSSLELSFLNGPLLVYLPYPPTW
                     TEVPDIPAG"
     gene            complement(6730..7611)
                     /locus_tag="PLM68_03010"
     CDS             complement(6730..7611)
                     /locus_tag="PLM68_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOT54367.1"
                     /translation="MKNKRSAILVLILLPLLAGCTEAITLRSEDMEPRLVITGTLSTV
                     TEYQVIEISSTAPYFGKDMPVNLNATEVRLNGEKLANDAPGIYSMGQEFAALEGETYL
                     LEVWIDYDRDGQDEYYTATTTVPYVHTLESLVLRSLIPSGNDTPFVLIAGFQDTPGED
                     YYGGALWINNVLYSNRILRYYIHTLNDYTQDGEFLRYPVPEWFITKSLVWDNGETFDL
                     YAGDVLTLELQTLSREYYKFLEEAKIEKNQQFPLFSGPRGNVTGNITGGALGIFGSYA
                     SSRKSVTLPECPGLPSR"
     gene            complement(7614..9977)
                     /locus_tag="PLM68_03015"
     CDS             complement(7614..9977)
                     /locus_tag="PLM68_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54368.1"
                     /translation="MRQYITSLFRKFPLLLGFIVMSVAGLQARTLYSLTGKVMDAVSG
                     DALPGAVIRLPEEQGAWTLTDTQGHFLLHIPEGTVRLEISYIGYNTKHTEIVLDRDID
                     TVFFLSPMTYNLQGLTVLADSPANRLRRSEMSIERLDVEVIQRVPTLFGEVDLLKVVQ
                     MLPGVQAASEGSSGFHVRGGSPDQNMILFDNATLYNVSHMLGFFSIFNTDAVKTMTLY
                     KGDIPARFGGRLSSLLEVIPSDGSGRFTVDGGIGLISSRLSVQGGIANDRLTYLASAR
                     RTYADLFLPLAKNEIIKNTTINFYDLNARLKWKLDDQNYLFMTLYNGRDRFSISDMGL
                     KFGNAAASLNWNHVISDRFLMQVFTTYSNYNYDFQASTSEMALEWISRIQDAGLRLDF
                     TYALNNENVFEFGYTSNFQWFQPGKAIGKGTLGSGTQVQQDITMSPRQGFVNVLYFSN
                     EHKLFGRRIELRYGLRFTRFDNVGPTTLYQVDENHNLLNKEGTEIPSGKFFHHQYGWE
                     PRLGFSFLVTPAISLKAAYSRTLQYVHLLSFSSAGSPMEIWIPSGPSILPQASHQVSA
                     GIFANLFNKEYEASAEVFYKHLDNVLDFKEHPDLLLYDKIETELRFGKGKSYGIELLL
                     KRNTGKLSGWISYTWSRSFRTIPGVNNGQTYRAPSDRPHNVNIVLFYDISPRLQASLN
                     WIYATGMPITLPEGRYWFYNELIPVYTERNSYRIADYHRMDASLNIRLGKPGKRWKNS
                     LNISIYNLYGRKNPWAINYRMNTLGEQYLEMTYLFSVVPSITWNFTF"
     gene            complement(10059..10316)
                     /locus_tag="PLM68_03020"
     CDS             complement(10059..10316)
                     /locus_tag="PLM68_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT54369.1"
                     /translation="MNKADLIAAMAAESGLSKVDSKKALDAFLAATTKALKKGERVTL
                     VGFGSFVVGKRGERTGRNPRTGATIKIKAKKVVKFRPSIKL"
     gene            complement(10369..11307)
                     /locus_tag="PLM68_03025"
     CDS             complement(10369..11307)
                     /locus_tag="PLM68_03025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOT54370.1"
                     /translation="MEWQTKVTVQPLPERFSYATRFFFAGSCFAIDIAQKMRDLHFLV
                     SPDAFGTLFNPASIASSLERLEVPLFFEQAEVMHFPYGAYGSLNHHTSFSGEDADSFL
                     AHANAQLKKASDFFLTADVVVITFGTAWTYTYQGKVVANCHKMPARFFNRDFLSPEKT
                     AELMTPLLQRHHNKTWIMTVSPIRHWGDGAHGNQLSKASLLLAIERLQDSFPNVRYFP
                     SYELVMDELRDYRYYAADMCHLGEETIRYILERFLEAAADEETRDLVKKMEKLNASLA
                     HKPLFPKSEQNFIFSKKLEKQRAELLQTIGNKRKLC"
     gene            11366..12895
                     /locus_tag="PLM68_03030"
     CDS             11366..12895
                     /locus_tag="PLM68_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOT54371.1"
                     /translation="MKKIILLLFCMILPMHYMFAQNDSIARLNTGNETHVVQGMTPPE
                     QALSQQTAETETSVVEEPVVEKVNPYKGILESRQFVFSIKSILRGLLGIACILLIAFV
                     FSTNRKKINWKTVGIALLMQLLIAIGILSVPAVQAVFEIFGKAFVVVLDWTKAGSNFL
                     FGSLMDLNKFGYVFALQILPTIIFFSALTSLLFYWGILQKVVWVMAWVFTKLMNLSGA
                     ESLSTTGNIFLGQTEAPLLVKAYVPTMTKSEIMLVMTAGMSTMAGGVLAAYIGMLGGG
                     DLQMSVAFAKHLLSASVMAAPGAIAMAKILVPQTEEIDNKVKVSKEKMGRNMLDSISN
                     GAIDGLKLAANVAAMLLVFYALIAGVNYIFGKVGHWTHLNGLIANWTNGMYDSLSLEC
                     VLSYLFAPVIWLTGVSGQDVALVGRLLGEKLIMTEFVGYSSLAQMIQAGMFVNTKSLI
                     IATYVLCGFANFASIGIQIGGIGGIAPNQKHVLARFGFRSLLGATLAALLSASMIGMF
                     L"
     gene            complement(12899..14011)
                     /locus_tag="PLM68_03035"
     CDS             complement(12899..14011)
                     /locus_tag="PLM68_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOT54372.1"
                     /translation="MDGKLKKRSYNFRITILDRYIIRKFMGTYFFAILIIIGIVIIFD
                     ISEKIDDFVDREAPLRAIVVDYYMNFIPYFMNMFSPLFVFISVIFFTSKLAGHSEIIA
                     MLSGGISFNRIMYPYILSALFIALLSLALNWFVIPPANRQRLVFEEQYLKTRTEYNRN
                     IHYQVEPDTYVYMESYNSWNSTAIRFTLESFQGNKLASKLSAETARWDSTTGRWRLNE
                     YNLRRFSDTSNTVTWGRQLDTLIDITGADLVRRKESVEQFNLRQLNQFIETLRLRGDT
                     NIKFALIQKNSRLAIPFSSFILTIMGVALSSRKRRGGIGLNIGIGIALSFSYILFLRF
                     SEMFVHADALSPAIALWLPNVLYALIAFVLYRLAPK"
     gene            complement(14011..15141)
                     /gene="tgt"
                     /locus_tag="PLM68_03040"
     CDS             complement(14011..15141)
                     /gene="tgt"
                     /locus_tag="PLM68_03040"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT54373.1"
                     /translation="MNFTVLHKDPASGARAGKLVTDHGEIDTPIFMPVGTLATVRGLF
                     HRDLVEDVKAQIILGNTYHLYLRPGLDVIKRAGGFHKFCSWDRPVLTDSGGYQVFSLA
                     AKRKLTPEGYYFQSHIDGSSHFFSPEKAVDIQRIIGADIMMALDECTPGDASYEYAKQ
                     SLAMTRQWLERGLERFRSTEGLYGYGQSFFPIVQGCVYPELRRQAAEHAASLDAEGYA
                     IGGLSVGEPTEVMYQMIETVQPYLPQDKPRYLMGVGTPANILEAIARGIDMFDCVIPT
                     RNGRNGMLFTRKGIINIKNRKWADDLSLLDPDGTSFVDKTYSKAYLRHLFTSGEMLGP
                     QIASMHNLAFYLQLVKEAAQHTRQGDYTPWKNKMVAQLEMRL"
     gene            complement(15425..16105)
                     /gene="rplQ"
                     /locus_tag="PLM68_03045"
     CDS             complement(15425..16105)
                     /gene="rplQ"
                     /locus_tag="PLM68_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT54374.1"
                     /translation="MRHRKKVNHLGRKEGHRKAMLANMASSLIVHKRIETTVAKAKAL
                     RVYVEPLITKSKFDTTHSRRTVFAYLKDKNAVNELFRVVGPKIADRPGGYTRVLKTGF
                     REGDAADMALIELVDFNELALAGSTKKEEKKTTRRGRPRGSKEKTGTTDKVTAAVGQA
                     SEQEQTEEEKTQPKPKAKTAKAKAKPAAAETVAEAVTEPAEEVDAEPAAEPAAEPAAE
                     PAAEPAAE"
BASE COUNT         4039 a         3897 c         3974 g         4211 t
ORIGIN      
        1 tttcagaaac tattggcgtc cgcccaggat taccgcatgg taatcgtccg ttgcaatatt
       61 attgtcgaaa taattataag gattgaccgg acgtcccata tatattacct cgtaatgtat
      121 atgagggcct gttgatttcc ctgttgctcc caggaggcct atctgctctc cgcgcttgac
      181 cagctgtcct tcatatacgg tggtcttgtt caggtgagcg taacgagtct tatagccaaa
      241 tccgtggtca atcagtaaat aacgtccgta accgttgtat tcgaaacgta tcgcttcaac
      301 tttcccgtca cccgttgcat acacaggtga attgggtgcc cccgtaaggt cgattccctc
      361 atgcatcctg atatcaccgt acacgggatc cttcctgtaa ccaaaacctc ctgaccacct
      421 gaaatttctt gtatcctcag gaataggcaa tatcgatgga atgcacttgg ccacttctcc
      481 tacctggccg gccacacgtt ccacgtcatc aaaagatttt gactgtatat aggccttctt
      541 catgatcctg tcataactca gggccgttgt caatagcaat ttggagttct ggacatactc
      601 cagatgggga taacggttgg accccccgaa tccggcattt ctgacctccc cggggatctg
      661 ttccatccca aatatattcc tgtagatgtt gttgtcccgg agttgcaggt tttccagcct
      721 tttttcagcc tgggtcatct gtttattaag aagttccaaa cctgactgtg cgttttcata
      781 ctccctttcc agcttgacca gcctgggagt tttcagtttg aatccctctg tgtagaccag
      841 gtaataaccc aacgccacca ccaggctaat gggcaggaga acgaaaaccc ttatcaggta
      901 aaagcgcaat gagttctttt gcagctcgta agaaagtgtt ttgggattat atttgtattt
      961 tctttttcct gccataagtg atgtaaacag gttattaacg agcaaagata agtatttttc
     1021 caaaaagcgg tatatttgtc ccttattatt gtagctatta tgacttcagg cgatattcgg
     1081 aagaccttcc tgaatttttt tgagaagaaa ggacatacta tagttccctc tgcacccatg
     1141 gtaataaaga acgatcccac cctcatgttt accaatgcgg ggatgaacca gttcaaggat
     1201 tggttcctgg gtaatgagcc tgctgcgcac aggcgcgtgg ccaacaccca gaaatgtctc
     1261 agggtgagcg gaaagcacaa tgatctggaa gaagtgggac acgacacgta ccatcatacc
     1321 atgttcgaga tgctgggcaa ctggagtttc ggcgattatt tcaagaaaga atccataacc
     1381 tgggcctggg aactccttca ccaggtattc aaactggatg cagaccgtct ttatgccact
     1441 gtgtttgaag gttcggacga ggacagcctg ggccccgatg atgaatcgcg tgccatctgg
     1501 gaggaattcc ttcccccgga acggatcctg gcgggcagca aaaaggataa tttctgggaa
     1561 atgggagaga cagggccatg cggaccctgc agcgaaatcc atgttgacct cagaagtccc
     1621 ggtgagcgag ccgaggtcaa gggagtatcc ctggtcaaca aggggcaccc cctggtcgtg
     1681 gaaatctgga atcttgtttt tatacagttc aaccgtaaaa ccgacgggaa actggtagca
     1741 ctggagcaaa aacacgtaga tacgggcatg gggctggaac ggttgtgcat ggcaatccag
     1801 ggaaaaacaa gcaattacga caccgacctg ttccagggac tgataaggtc cattgggttg
     1861 ttatgcaaca aggaatatgg aaaggatgac caggtggatg tggccatgcg tgtaatctca
     1921 gaccacctca gagccatatg cttttccata gccgacggac agctgccctc gaacgtgaaa
     1981 gccggttatg taatcaggcg catcctgcgc agggcggttc gttacggaca ttctttcctg
     2041 ggttttgaca agccattcct ttgtgaactg gttccctccc tgatacaaca gatgggagaa
     2101 gcgttcccgg agcttattca gcaacagggc ctgatccaga acattatccg ccaggaagaa
     2161 gaagcttttt tacgggccct ggaaaagggg acccgtttat ttgaatattt tgtccgtgac
     2221 aagacactga aagagttcag cggcaaggac gccttcctcc tgtacgacac ctatgggttc
     2281 ccgcttgacc ttaccatcct tatggccagg gaaaagggac tggcagtgaa tattgctgaa
     2341 ttcgaggaag aattgcaggc acagaaagac agggcacgta aggccaccct tgtaaaagaa
     2401 ggagactggg ttgaggtacg cccgttcata caaccttcat tcctcggata tgagaccacg
     2461 gagacttttt cccgtatcac ccgctacagg aaagtcaggt caaaaaaccg tgaattctac
     2521 catattgtat tggaccagac gcctttttat gcccagagcg gaggacaggt gggcgattca
     2581 ggctacctgg aaaacaccca gggagagaaa atccctgtta ctgacaccat caaggaaaac
     2641 aacctgattc tgcatatttc gccaaatgcc ccggaaaatc cggagacgga attccgtgct
     2701 gtcgttgacc gggaacgcag gcagaccatt gccaataacc atactgccac ccacctgctt
     2761 cattatgcgc tcagacaggt gctcgggccg caggttgaac agaaagggtc tcaggtgtcg
     2821 gccgagggat tgcgtttcga tttttctcat tatgcccgga tggaaccgga agaaatccgg
     2881 aaaaccgaac gtattgtgaa tgatctgatc cgcaaaaaca tagtccagga tgcgttgcag
     2941 gacattcccc tggaagaagc aggacagatg ggtgccattg ccctgtttgg ggaaaaatac
     3001 ggagcaaagg tccgggtgat ccgctttggt gaatccatag aactgtgtgg cggaacacat
     3061 acttcggcca caggcaatat cgggatattc aagatagcga gtgaaggcgc cattgcagcc
     3121 ggcgtacgca gaatagaggc tatcacaggc cttgccgtgg aacggaaaat tgaagaaatg
     3181 gaaaccatgc tcctgggtgt caagaacctg atgaatgacc atcccgatat cattgcctcg
     3241 gtgcaaaaaa tgttgcatga aaacgaagac ctgcataaag tggtggaaga aaacaggcgt
     3301 gaacgggctg tatccctggg aaaatcactc tgggaacaac gctccagcct gaacggcatt
     3361 caatttgtac acctgcgggg tgtatacatg cccgaaatga taaaagacat ggcgttctct
     3421 ttccgcgaag gcaactacga taacatggct tttgccgcag cctatgaaca taacggcaag
     3481 gccaacctga ccctgcttct ttccaaggac cttgtcagcc agggtttgca tgcggcagag
     3541 attgtccgta atgcagccag gcatatagga ggaggcgggg gaggacaaga tttttatgca
     3601 actgccgggg ggaaagatac gggaggactg caggcagccc ttgatgccat agccgaagca
     3661 ttgaagaacc attaaaccca ctggcggtga aaatattcga ccgtttcctg gtccgctctt
     3721 tccttgggcc cttgttcctg actattttca tagtcctgtt cgtgcttgtg ttgcagtttc
     3781 tctgggtata catagacgaa ctggtgggaa aagggctggg attgaaggtt atcctggaat
     3841 tcctcttctg gggaacctgc accgtcattc ccatggcgct tcccctggcc accctgctgg
     3901 cttccatcat gaccatgggg aaccttggag aattcaacga attgctggcc atgaaagcgg
     3961 caggaatttc attgcagcgc attatgcgtc ctctcattat tctttctttt cttgtttctg
     4021 tggttgcttt tttttctgcc aacaacctga tgccactggc ttacaagaat attttcgcat
     4081 tacgggatga catatcccgt accaaggagg aaatccgtat tcctaccggt atcttttaca
     4141 acgggataga caattacagc ctccgggttg aacacaggga caaggcaacc gatatgctgt
     4201 atgatgtaat ggtttacaac cacagctcca gaaggggaaa cagcagtgtg accctggccg
     4261 attccggatc catccggctt accgaagaca agaaaaatat tcttttcacc ctgttcagcg
     4321 gagaaacata tgaggagggc gaaaggaaat cagttgccga tacttctttt gccatgcaac
     4381 ggattaaatt tgaaaaacag gagatcgtca ttgaactgac caattatgcc tttcaaagac
     4441 gtgacggaga tcgctttgga agcgacgtga tggcacaggg ggtccgtaat ctgggccacc
     4501 ggcgtgactc gctggactct ctctatacca tttccctgga cctgtttcaa aaacgaatgc
     4561 tcatagccgg gggactggct tttgcacgtc agatggacac ttcagacagg caacagtata
     4621 agggggattt ccccattgaa cagcttcccg atccctcaaa aagcacgcag tatcccgacc
     4681 tgttgaaccg ggccataggc aaggtggacc aaatgataaa ccaggtggac aattacatga
     4741 tagaagaagt ccagatacac cttcccctga gaagaacaaa agttgagtgg taccgtaaat
     4801 tcacagtggc ttttgcctgt tttatcttct ttttcatagg agcccccctg ggagccatca
     4861 tccgtaaagg aggactgggc acaccggtga ttgtttccat gctgtttttt gtggtctatt
     4921 acgtgattga catatccgga aaaaagctgg ccacagacgg atcggtaacg gctgccatgg
     4981 gcaccctcat atcatcgctg gtcttgcttc ccctgggggt gttcctgact tataaagcca
     5041 caaccgactc ggctttattc aacaaagatg cgtatcttgt gatgatcagg aaaatcagga
     5101 agcatttacc acttcgccat gataaagaaa acgaacaagc ctgacattgt ttttatggga
     5161 actcccggtt ttgcagccgg ggtattgctg gagctcctgc ggcagggata tcctgtaaag
     5221 ggcgttgtga ccgctcccga taaaccctcc ggcaggggtc ttgtcatgcg cccttcggcc
     5281 gtgaaaatga cagcactgga acacaacctg ccggtgctgc agcccgtttc gctgaaagac
     5341 gaaggttttc tggaaagtct tgccctatgg gacgctcctg ttttcgtggt tgtggctttc
     5401 aggatgttac ctccccaggt ttggcaaatg ccatctcacg gatgtctgaa cctgcatgct
     5461 tccctgcttc ccatgtaccg gggcgcggcc cctattaacc acgccctgat caatggggaa
     5521 aaagaaaccg ggttgacaac ctttctaatt gatgaacaaa tagatacggg agcaatactc
     5581 ctgcagcacc ccatccccgt ggaagagcgt gacaatgcag gatccctcca tgataaaatg
     5641 atggaagccg gtggtcccct ggtggcaaga accattgacg gcctctggtc gggcagccta
     5701 aaaccggtgc cacaaccatt cgttgcgcca gaagaggtga agccggcacc ccggataagc
     5761 agggaaacct gccgtattga ctggaaccgt gaacccggac agatcgtgaa tcttgtcagg
     5821 gggctgagtc cctatccctg tgctttctca accttgaaga aaggggatac cctgatggac
     5881 atcaaggtgt atgaagcccg ggccgaaatt gtcctgcatg gcgattcccc gggcgaagtt
     5941 gtctcggact ggaaaacaac cataaaaatc gcctgctgcg gaggatatgt ccatttgctg
     6001 gaagtccagg ctccgggtaa aaaacggctg gctgccggtg cctttcttgc aggattcagg
     6061 aacacagaag aactgacttt tctatgaaaa tgaaagaagc cgttattctg gcccaggggg
     6121 catatccttc acacccttac ccgttacacc tgctggagac ggccgggacc ctcatttgta
     6181 ctgacggggc cgtgaataaa ctgcagggac gggaacccga ccgggttgtg ggtgaccttg
     6241 attccttgtc ggaaacgtta cgggaaaaat acagctcacg gcttgtgcag gatatatccc
     6301 aggagacaaa tgatttgaca aaagccgtgc aatattgctt ccgggaaggt ttccggagca
     6361 ttgtcatcct gggggccacc ggtctcaggg aagaccacac actgggaaac atctcccttt
     6421 tgggtactta cgcacagatg atggatacag tgcgcatggt gacagatcat ggtgtattct
     6481 ttgtttttgc ccggggaaca caaaaggccg atggcttcat ggaggtgaat atcccctgcc
     6541 cggcgggaaa tcccgtctca ttctttgcca tcaaccccgc aatgaggtta cgcgcgaagg
     6601 gtgtgcatta tccggtggaa agcgtggtct ttgactcctg gtggaaagcc acgctcaatc
     6661 gttgtacggg aagcagcctg gagttgtcct ttctgaacgg gcctttactg gtttaccttc
     6721 cctatccccc tacctggacg gaagtcccgg acattccggc agggtaaccg acttgcggct
     6781 ggacgcatac gatccgaata ttcccagcgc accacctgtg atatttcctg taacattgcc
     6841 cctgggacct gaaaaaagcg ggaattgctg gttcttttct attttagctt cttcaaggaa
     6901 cttataatat tccctgctca aggtttgcaa ctcaagggtc agcacatcgc cggcatacag
     6961 atcgaaggtc tccccgttat cccataccag tgacttagtg atgaaccatt ccggaacggg
     7021 ataccgcagg aattccccat cctgcgtgta atcgttcaag gtatggatgt aatacctcag
     7081 gatacggttg ctgtacaaca cgttattgat ccatagtgcg cctccgtagt aatcttctcc
     7141 cggcgtgtcc tggaaaccgg ctataagaac aaaaggtgtg tcatttcctg aaggtatcag
     7201 tgacctgagt acaagggatt ccaaagtatg cacatagggt acggtggtag ttgccgtgta
     7261 atactcatcc tgaccgtccc ggtcataatc tatccagact tccagaagat atgtttctcc
     7321 ctccagtgct gcaaactcct gtcccatgga ataaataccc ggggcatcat ttgccagttt
     7381 ttctccattc agacgcacct ccgtggcatt cagattcaca ggcatatcct taccaaagta
     7441 aggggctgtg gaagaaatct ctattacctg gtactccgtg acagtggaca aggttccggt
     7501 aatcaccagt ctgggttcca tgtcttcact tctgagcgtt atagcttcgg tacagccagc
     7561 cagcaagggc agcaggatca aaaccagtat agcagatctt ttgttcttca tgactaaaaa
     7621 gtgaaattcc atgttattga ggggactacg ctgaaaagat aggtcatttc aaggtactgc
     7681 tcccctaagg tattcatccg gtaattgata gcccaggggt ttttgcggcc gtaaaggttg
     7741 tatatggaga tattcagtga atttttccag cgctttcctg gttttcccag gcggatgttc
     7801 agcgaggcgt ccatgcggtg gtaatcggct attctatagg aattccgttc tgtatataca
     7861 gggatcagtt cattgtagaa ccagtaacgg ccttccggca gggtaatggg catgccggtc
     7921 gcatagatcc aattgagcga agcctgcaag cgtggcgaaa tgtcataaaa gaggacaatg
     7981 ttaacattgt gcggacgatc cgaaggagcg cggtatgtct gaccgttgtt gactccgggt
     8041 atggtcctga aagagcggga ccatgtatag ctgatccatc cgctcagctt cccggtattt
     8101 ctcttgagca acaattcaat cccgtaactc tttccttttc caaaacggag ttcggtctcg
     8161 atcttgtcgt acagtaggag gtctggatgc tccttgaagt caagcacatt gtccaggtgc
     8221 ttgtagaata cctcggccga ggcttcgtat tctttgttga aaaggttggc aaagataccg
     8281 gcagatacct ggtgggaggc ctggggcagg atggaaggcc ccgacgggat ccatatctcc
     8341 atgggtgatc cggctgaaga aaaagacaac aggtgaacat actgcagtgt tctggaatag
     8401 gcggccttca atgaaatggc gggggtaacc aggaatgaga aacccaggcg gggttcccat
     8461 ccatactggt gatgaaaaaa ttttcccgaa ggtatctctg tcccttcttt attaagcagg
     8521 ttgtgatttt catctacctg gtacaacgtt gtgggaccca cattgtcaaa acgtgtaaaa
     8581 cgaaggccat accgtagttc gatccttctc ccgaacagct tgtgctcgtt tgaaaaatac
     8641 agcacattca caaaaccctg gcggggagac atggttatat cctgttgcac ctgtgttccc
     8701 gaccccaatg ttcctttccc gatagctttt ccgggctgaa accactggaa atttgacgta
     8761 tatccgaatt caaaaacgtt ttcattgttg agtgcataag taaaatccag gcgaaggcct
     8821 gcatcctgga tacgggatat ccattccagg gccatctcgc tggtggatgc ctgaaaatcg
     8881 taattgtaat tgctgtaggt agtgaaaacc tgcattaaga aacggtctga gatcacatgg
     8941 ttccaattga gagatgctgc ggcatttccg aatttcaagc ccatatcaga gatgctgaat
     9001 ctgtcacgcc cgttgtacag agtcatgaag aggtaatttt gatcatcaag cttccacttg
     9061 agacgggcgt tcaaatcgta aaagttgatg gtcgtgtttt ttatgatctc attctttgcc
     9121 aggggaagga acaggtctgc atacgtccgg cgggcggaag caaggtaagt cagccggtca
     9181 tttgctatac ctccctgaac cgagaggcga ctggatatga gacctattcc cccatcaaca
     9241 gtaaaccttc cggatccgtc ggaaggaatg acctccagca aagaagaaag ccggcctcca
     9301 aaacgcgctg gtatatcccc tttgtagagt gtcatagttt tcactgcatc agtattgaat
     9361 atggaaaaaa agccaagcat gtgggacaca ttgtacagtg tggcgttgtc aaagagtatc
     9421 atgttctgat cggggctgcc tccgcgaaca tgaaaaccgc tgcttccttc cgaggcggcc
     9481 tgtactccgg gaagcatctg taccactttc agcaggtcca cttctccgaa caacgtggga
     9541 acacgctgaa tgacttccac atccaaacgc tcaatgctca tctctgacct tcttagcctg
     9601 ttggcaggac tatcggccag taccgtcaga ccctgcaggt tgtaggtcat gggggaaaga
     9661 aagaataccg tgtctatgtc cctgtcgagg actatttccg tatgctttgt attatacccg
     9721 atataggaga tctcgagccg tactgttcct tcaggaatgt gcaacaggaa atgaccctgt
     9781 gtgtcggtaa gtgtccaggc cccctgttct tcaggcaacc tgatcacggc accgggcagg
     9841 gcatctccag atacagcatc catcactttc cctgtaagcg agtatagcgt acgggcctgt
     9901 aatccggcaa cagacatgac gatgaatccc aaaaggagag ggaactttct gaacaaggaa
     9961 gtgatgtact gcctcatgga tcgtttacat acataaaaag gagaacatgg gtgtcctcct
    10021 tatcttatcc tgactaatgt caagctaatg taccgtggct acaatttaat actgggacgg
    10081 aatttaacga cttttttagc cttaatcttg atagtagctc ctgtacgagg atttctccct
    10141 gttctttcac cccttttccc tacaacaaat gagccaaacc caactaaggt aactctttcg
    10201 ccttttttca aggctttggt tgtggcagca aggaaagcat ccaatgcttt ttttgaatca
    10261 acttttgaca gtcccgattc agcagccatt gcggcaataa gatctgcttt gttcatgata
    10321 ataaatatta ggttaaatga aagttttcta gtggtaacag cgacaaatct aacataattt
    10381 tcttttgttt ccaatagttt gcagtaattc agccctttgt ttttcaagtt tttttgaaaa
    10441 aataaagttt tgttcagatt ttgggaaaag gggtttgtgt gcaagagagg cattcagttt
    10501 ctccattttt ttcacaaggt cccttgtttc ttcatctgca gccgcttcca gaaaacgttc
    10561 cagaatataa cgaatggtct cttctcccag gtggcacatg tcggcggcat aatacctgta
    10621 gtctcttagt tcatccataa caagctcata ggaaggaaaa tagcgcacat ttgggaaaga
    10681 atcctgcagc ctttcaatgg caaggagaag ggatgctttg ctcaattggt taccgtgagc
    10741 tccatctccc caatggcgga tggggctcac ggtcatgatc catgttttgt tatggtgccg
    10801 ctgcagcagt ggtgtcatca gttccgccgt tttttcaggc gacagaaaat cgcgattgaa
    10861 aaaacgtgcg ggcattttat ggcagtttgc cactaccttg ccctgataag tatacgtcca
    10921 ggctgttcca aaggttatca ccaccacatc ggcagtaagg aaaaaatccg aggctttttt
    10981 cagttgagca ttggcatgag caagaaaact gtcagcatcc tctccggaga aagaggtatg
    11041 gtggttcagg gatccgtatg ccccataggg aaaatgcatc acctcggcct gttcaaagaa
    11101 aagagggacc tcaaggcgtt ccagtgatga agctatggat gcaggattga acagcgtgcc
    11161 aaaggcatcg ggagatacca gaaaatgcag atcgcgcatc ttttgtgcaa tatcaatggc
    11221 aaaacaggat cctgcaaaga aaaaccgcgt tgcataggaa aaacgctccg gcaggggttg
    11281 gactgtcact tttgtttgcc attccatagt cacaaagtta gcattttgtt tatctttgac
    11341 acgttaatcc aaaaactacc cctttgtgaa aaaaatcata cttctgctgt tttgcatgat
    11401 attgccgatg cattacatgt ttgctcaaaa cgactcgatt gccagactca atacaggaaa
    11461 tgaaacgcat gtggtgcagg ggatgactcc ccctgaacag gccctgtccc aacaaacggc
    11521 tgaaactgag acatccgttg tggaggaacc tgtagtggaa aaagtgaatc cttacaaagg
    11581 catcctggag agccgccagt ttgtctttag catcaaatcc attctcaggg gtcttctggg
    11641 tattgcctgc atactgctga ttgcctttgt tttcagcact aatcgtaaga aaatcaattg
    11701 gaaaacggtc gggatagctt tattgatgca attgctcatt gccattggca ttctttctgt
    11761 ccccgcagtg caggcagtct ttgaaatatt cggaaaagcc ttcgtggttg tcttggactg
    11821 gaccaaggct ggttccaatt tcctgttcgg atcattaatg gatctgaaca aattcgggta
    11881 cgtgttcgcc ctgcagatcc tgcctaccat catctttttc tctgccctta catccctgct
    11941 gttttactgg ggtattctgc aaaaggttgt atgggtcatg gcctgggtat tcaccaagct
    12001 gatgaacctc tctggggctg aaagcctgtc aacaaccggg aatattttcc tgggacaaac
    12061 agaggctccc ctgctggtga aagcctatgt ccccaccatg accaaatcgg agatcatgct
    12121 ggtgatgacc gccggcatga gtaccatggc aggaggggtc ctggccgcat acattggaat
    12181 gctgggtgga ggggatcttc agatgagcgt tgcctttgcc aagcacctgc tttctgcttc
    12241 ggtcatggct gcacctgggg caattgccat ggccaagata ttggtcccac aaacagaaga
    12301 aattgacaat aaagtcaagg tttcaaaaga aaaaatggga agaaacatgt tggattccat
    12361 ttccaacggg gccattgacg gattgaagct tgcggccaac gtagctgcca tgttgctggt
    12421 cttctatgcg ttgattgccg gtgtcaatta cattttcgga aaagtggggc actggaccca
    12481 cctgaacgga ctgattgcca actggaccaa tggaatgtac gacagcctct cgctggagtg
    12541 tgtcctgtct tacctttttg ctcctgtcat ctggcttacc ggtgtttcag gacaggatgt
    12601 tgcccttgtg ggccgtttgc tgggagaaaa gctcatcatg accgaatttg tgggttacag
    12661 cagccttgcc cagatgatac aggccggaat gttcgtaaac acaaaatctt taattatagc
    12721 tacttatgtg ctttgcggct ttgccaactt cgcttctatc ggtattcaga ttggagggat
    12781 aggtggcatt gcacccaacc agaagcatgt gctggcaaga ttcgggttcc ggtcactcct
    12841 gggagccacc ctggccgcgt tgctgtcagc ttccatgatc gggatgttcc tgtagttatc
    12901 atttaggtgc caggcggtac aacacaaagg ctatcagcgc ataaaggacg ttgggcaacc
    12961 aaagggctat ggccggggac agcgcatcgg catggacgaa catttcagaa aatcttaaga
    13021 acaagatata ggaaaagctc aatgcaatgc ctatcccgat gttcaatcct atcccgcccc
    13081 gtctcttgcg tgaggacaag gctacaccca ttatggtcag tataaaggaa gaaaagggaa
    13141 ttgccaggcg cgaatttttc tggatcaggg caaatttgat gtttgtatcg ccacgcaggc
    13201 ggagggtctc aatgaattgg ttcagttgtc tgagattgaa ttgttccaca gattcctttc
    13261 ttctcaccag gtcggctccg gtgatatcta tcagggtgtc cagctgtctt ccccaggtga
    13321 ctgtgttgga tgtgtcggaa aaacggcgga ggttgtattc gttcaaacgc catctccctg
    13381 tggtggaatc ccagcgtgcc gtctcggctg aaagtttgga tgcaagcttg ttaccctgga
    13441 atgactcaag ggtaaaacgg atggccgtac tgttccagga gttgtaggac tccatataga
    13501 cataggtgtc cggctccacc tggtaatgga tattcctgtt atattctgta cgcgttttca
    13561 gatactgttc ctcaaagacc agtcgctgcc tgttggctgg tggtatcaca aaccagttca
    13621 gggccaggga taacagggct atgaacagcg cagacaggat ataggggtac atgatcctgt
    13681 tgaaactgat cccgccgctc agcatggcaa tgatctcgct gtggcccgcc agcttggatg
    13741 tgaaaaagat tactgaaatg aagacaaaca ggggcgagaa catattcatg aaatagggga
    13801 tgaaattcat gtaataatcg accactatgg cccgcagagg ggcttccctg tctacaaaat
    13861 catcaatttt ctcactgatg tcaaaaatga tgactatgcc aataatgatg agaatggcaa
    13921 agaaataagt ccccatgaac ttccggataa tgtaccggtc cagtatggtt atgcgaaaat
    13981 tataggacct tttcttcagt tttccatcca ttacaacctc atttcaagtt gtgccaccat
    14041 cttgtttttc catggagtgt aatctccctg tcttgtatgt tgtgcagctt cctttaccaa
    14101 ttgcaaataa aacgccagat tatgcatgct ggctatctgg ggccccagca tttcccccga
    14161 agtgaaaaga tggcgcaggt atgcctttga ataggtttta tccacaaatg atgttccgtc
    14221 cggatccaga agagacaggt catcggccca tttcctgttc ttgatattga taatcccttt
    14281 cctggtgaac agcattccgt ttcgcccgtt ccttgtaggt atcacgcaat caaacatatc
    14341 tattcccctg gcgatcgctt ccagaatgtt tgccggtgtc cccaccccca taagatacct
    14401 gggtttatcc tgtggcaggt aaggctgtac ggtttctatc atctggtaca tgacctctgt
    14461 gggctccccc acggaaagcc cgcctatggc ataaccttcc gcatctagcg aagctgcatg
    14521 ttccgctgcc tggcgacgca gttccgggta cacacatccc tgtactatgg ggaaaaaaga
    14581 ttgtccgtat ccgtagagtc cttccgtgga acgaaaacgc tccaaacccc gttcaagcca
    14641 ttgccgggtc atggctagtg attgtttcgc gtactcgtaa gaggcatcac ccggcgtgca
    14701 ttcgtccagg gccatcatga tgtcggcgcc tatgatcctc tgaatatcaa ctgctttttc
    14761 cggtgaaaag aaatggcttg acccgtctat atgcgattgg aaatagtatc cttcgggagt
    14821 aagtttcctc tttgcggcca gtgaaaaaac ctggtatcct ccactatcgg taagtaccgg
    14881 cctgtcccaa ctgcaaaatt tatgaaatcc tcctgccctc ttgataacat caagacccgg
    14941 cctcaaatac aaatgatatg tattgcccag gatgatctgc gctttcacat cttcaaccag
    15001 atctctgtga aacaggcctc ttacggttgc caacgtgcct acaggcataa aaattggtgt
    15061 atctatctct ccgtgatccg tgaccagctt cccggcacgg gcgccactgg ccggatcctt
    15121 atgcaacacc gtaaaattca tctcacaaat ataatccttt tactttttct ttctatctcc
    15181 aggttgctaa tgcggatggt tgcttcactg ctagaacgaa gcgcaataat ttaagccgag
    15241 gcgctctgtt ctaacagtga agcgacctgt atagttctct gtgcttttgt ggaattgcga
    15301 ccgacacaag aaaaagagac tgaccaatca tttgtgttgg tcagtctctt tttggtcatg
    15361 ctgcagcttc tgttgccggt agtcctgtgt gttcgcctgc aagttagccg gcagtactgc
    15421 cttgttattc agcagcaggc tcagcagcag gctcagcagc aggctcagca gcaggctcag
    15481 cagcaggttc agcatcaact tcttctgcag gttctgtgac agcttcagca accgtttcag
    15541 cagctgcagg ttttgccttt gcttttgctg ttttggcttt cggcttgggt tgagtctttt
    15601 cttcttccgt ctgctcctgt tctgatgcct gtccaacggc tgcagttacc ttgtccgttg
    15661 ttcctgtttt ttctttggaa cctctggggc ggccgcgacg tgttgttttc ttttcttctt
    15721 ttttggttga cccggcaaga gccagttcat tgaaatctac cagttcaatc agggccatgt
    15781 cagcagcgtc gccttcgcgg aatcctgttt tcaaaacgcg ggtataccct ccgggacggt
    15841 ccgcaatctt gggccctaca acacgaaaca attcgtttac agcatttttg tctttgaggt
    15901 aagcaaaaac cgtacggcgt gaatgtgtag tatcgaattt tgacttggtg atcagcggct
    15961 ctacataaac tctcagtgcc tttgctttag ctacagtggt ttcaatgcgc ttatgaacga
    16021 tcagggacga tgccatgttg gccaacattg ccttacggtg tccttctttg cgtcccaggt
    16081 gattaacttt ctttctgtgt ctcatttttc cttactttct t
//
LOCUS       DAOSOF010000030        15972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3695_length_15972_cov_5.452095, whole genome shotgun sequence.
ACCESSION   DAOSOF010000030 DAOSOF010000000
VERSION     DAOSOF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_15972_cov_5.452095"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PLM68_03050"
     CDS             complement(<1..1521)
                     /locus_tag="PLM68_03050"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOT54375.1"
                     /translation="MKLKYLLLSFFAFAALLTGCKEEIDIPSLDEVKVDMSYIAFAKE
                     GGTRTITLTATDSWDITNIPTWLTVSPASGSASPEGIKVTFTTAATDSDKSSEVYVNC
                     AGKVQVLTVKQIAGPQEIVISTCKDVIDKGVDGKIYWVEGQVTSIANTVYGNWYLNDG
                     TGTLYIYGTLDAKGNTKNFLSLGLSVGDRVMVHGPRLAYGSTIELVDVTVDKITPSLI
                     KSAVDAVQVPKEGGTFEVAVLYKGNGLEIEYDADWLSVKSMIKGADDSTRVAFVAQPN
                     AGGDRTATISMTSATNSNSSTITITVKQTGAIIDATVAHFIAAAEDATQYRISGIITK
                     DTGSDYGNIYIKDATGEVYIYGVLDAEGKAKQWKNMGIKVGDIVTLITPRGSYKGTPQ
                     GVNAKVETHIPVVDATVATFLAAAEDATYYRLTGTTGDLSGAGKYGNFNISDETGQVY
                     VYGLLSGWNGAQGLFQTLVTETGLKAGDLLTIVGKRTSYKGTPQVGSGFYVSHEPAA"
     gene            complement(1540..4827)
                     /locus_tag="PLM68_03055"
     CDS             complement(1540..4827)
                     /locus_tag="PLM68_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54376.1"
                     /translation="MKKVIQRFLALVAFLFLCVGAYAQVTTSSLGGRITDEAGAAAAG
                     VTLVATHVPSGTMYAAVTNNDGRYTIQGMRPGGPYKVEISYIGYQTMSFTDITLQLGD
                     LYNLSAELKEETFQLEDIVIVASASSAFAGEKFGSSTNISNQNIQTLPTISRTITDIT
                     KLSPYGGSGMSFSGGDGRSSNFTVDGANFNNNFGLSSSLPGGGTPISIDAIEEVQVVI
                     APFDVRQTNFIGGGVNAITKSGTNTLRGSAYIYHRNENMRGNTVDGVELGDRGIDRNT
                     TYGATLGGPIVKNKLFFFVNFEYTQTPTIVNRWRPSVDGVANTDLYISRTTMADMKRA
                     SDYLMSKYGYDTGSYTDYPANESNMKILARLDWNISQNHNLAIRYNYTLNRGWNNTNA
                     SSSNCIQRTTESRLGQYSMAFANSMYSMDNIVNSVSVDLNSRFGDNISNQLLVTYSQL
                     DDIRGSNSAKFPFIDILEGYTVDNGKISQAIVPYMSAGYELFTWNNGVHNTVINAKDD
                     ITLYKGNHKITAGLSFEYQMADNSYMRNGTGYYRYRSLDDFLSGAVPETVALTYGYDG
                     EQNPAARVRFNQFGLYLQDEWSIGRNFKLNYGVRLDEIIFNNKDLMRNNAIYAIEYDG
                     KSVDTGKWPKPKLQVSPRIGFNWDVFGNRSLKIRGGSGLFSGRLPLVFFTNMPTNSGM
                     VQNIVAISTKYSGAIANPDALLAAFQGDMITDVDALIEKLHSLDPTKFPKTISPEDGV
                     KPSQINGIDPNFRMPQVWKSSIALDYNFPTSFPFSISGEYIFNKTINGIQLLNWNIKE
                     NAGFTQLNGPDRRHIYPANYKYTSTDAFVLTNTKKGYGWTGSFTLNMTPVENLHIMAS
                     YTHTVMKQITGMPGSNAESAYLYIPTVEGPNFAGLSNSQYVTPDRIIASVSYKDRSNN
                     HFSLLYEGYRGGSNYTYLYNGDFNGDNIAYDVMYIPKDDSEILFASQSDRERYWAYAD
                     QDAYLSKNKGKYAEAYSVYSPWVHRFDFRYAHDFVVRIGKTRNALQLNVDFQNIGNLF
                     NSTWGVHQILSTEAMSGRILQLDKINEQGVPVFKTRVGAGARTWDYSHAIGQCWSLQV
                     GIKYLFN"
     gene            complement(4936..7014)
                     /locus_tag="PLM68_03060"
     CDS             complement(4936..7014)
                     /locus_tag="PLM68_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOT54377.1"
                     /translation="MHLRQFSKVLILPAFLLVSCGEKPVPQSLSLDKTQVTFAAAAGE
                     QAVQVSANCSWSVAVTEGSNWLSVTPGSGQGNATLTLSVQVNTGADRTGAIKVYSAEK
                     EVRITITQKEEETLYWSISQLRALYKGTDVKVTDKVIVKATVISNYLHADNEGLNNYT
                     SMKAIVVSDKNAGIQLFCAENNTDFKQGDKVEIQLQGQTLSVYNNGPLQVNGLPLAKI
                     TKTGTETLEVKEISAAQLITGQYESMYVAIPKVQVRDEDLGKTFVMESAHTSIGMIAE
                     TKETFDIFSSRYSSFKDVIVPSGCGVIKGIASVYDKKYQISFARVSDWEGLTGERFYT
                     SPTFTLYTSEKEIPGEEGQFTVSVAGNVDWTVISSDPENFAVSPASGSKTAPVTVTYT
                     QNPSETKPREANIVFSTTNTSIEQKTLTLHITQSPFEALISDPVRSWLELPDLSGMQE
                     EKNFAYISHMTSLRGKSVRNYSFWYDTQNRLSHWVAYPLYRDIMGSGSRSDAWNYNPK
                     VPKRYQPVLFYSFGGSSWDRGHQLPSADRLSSQEINESTFYFTNIAAQDASLNQNLWG
                     NLETYIRGWSLACDTLYVVTGAMIQTREDDSIDYVKDNDGNQVAVPKLYFKVLLKYKA
                     GQAENGGYSAIGFWYENRAYTASYPVASDAKTVDDMEDLTGFDFFHNLPDNIEKEIEK
                     VCTPGDWGLN"
     gene            7288..8499
                     /gene="metK"
                     /locus_tag="PLM68_03065"
     CDS             7288..8499
                     /gene="metK"
                     /locus_tag="PLM68_03065"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT54378.1"
                     /translation="MRTLFSSESVSEGHPDKIADQIADAILDEFLRLDPESKVACEVF
                     VTTGLVIIGGEVTSNAWVDIRDIARKVIADIGYTRAEYKFDSESCGIISLIESQSPDI
                     YQGVVREKPDEQGAGDQGMMFGYACTETEELMPLPIVLAHITLMELAAIRKEGKQMTY
                     LRPDSKCQFTIEYDEKQKAQRIDTLVLSTQHDPFAEEHLMHRQITEDVLNILLPRVID
                     KVHPSRKHLFEHDLKLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGRGAHGGGAFSG
                     KDPSKVDRSACYAARHIAKNLVAAGAAEEVLVQLAYAIGVAQPVSVFVNTFGTAHTKK
                     SDAEIAQIIAKVYDLRPARIIEELRLKYPIYKATAAYGHFGRDPFSKNGIDYFSWEKL
                     NYAEVFKSRLF"
     gene            complement(8491..8877)
                     /locus_tag="PLM68_03070"
     CDS             complement(8491..8877)
                     /locus_tag="PLM68_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54379.1"
                     /translation="MKRILWITLCLAVMSSCSCRQEPEQVALDFLQSFFASDYKQARV
                     YAAPEIMGILDKSQSIFDSLTPEEHQEVKDYLSAINITVEAPEKIKGDTVRLPYRVEF
                     SRLKEPGESFITLRREGRNWKVYALE"
     gene            complement(8874..11387)
                     /locus_tag="PLM68_03075"
     CDS             complement(8874..11387)
                     /locus_tag="PLM68_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT54380.1"
                     /translation="MIYPDQFEQKIGFDRIREQLTQLCMSSFGRELVKDMVFLTDFDT
                     LETSLERTGEMREICLFEQQFPEDGYEDTLYFLKQMESNPAYYLLVPDLVRLQRAIGT
                     VRALLHFFEHTRQESYRTLKDLCRPISFFPAIVQKIEHILDKSDEIRDNASPELAGIR
                     KSLKNKEQLVVQKIQSILKESRDQGITDPDASASIRDGRMLIPVPAANKKKIQGYIID
                     ESATGKTVFIEPMEVVNLNNQIRELEFAQQREIIRILQEFAGFLRPYVPELTEQARFL
                     GTIDFIRAKARMALKMQAGKPILNEDTGLYLIRARHPFLETALQREGKQIVPLTLRLD
                     AAKRILLISGPNAGGKSVCLKTVGLLQYMFQCGLLVPAAETSEFPIFRSIFLDIGDEQ
                     SLDNDLSTYSSHLLNMKEILEQADERSLILIDEFGAGTEPAAGGAIAETLLRRWELCG
                     SYGVITTHYTNLKFFATTSKGVLNGAMQFDVQNIQPLFKLETGVPGNSFAFELARKMG
                     LPADLIQEAQEKAGAGFVETERYIRSIARNRRKWEEKVARISQTDKTLENITGRYQAE
                     LEEIRLLRRKMLDEARKEALSIVETANRKIENTIREIKESQAEREKTKSAREDLQTFR
                     KELSQEAQDAEDEKILRKMEQLRKRKEAGEKRKQQREKLSGKEGKTRQKAQESDEDKG
                     PLKAGDKVRTKDGALVGEVLRIKGNKAYIGMGQIVTWTEITVLERVSQNEYRSLTRMQ
                     GSPKVVIPDDLQARRLHFFPHLDVRGLRAGEALEQVTRFLDDALVLGMEQVDILHGTG
                     TGALRTEIRNYLKTAPGVTSFQDEHVERGGPGITVVYLK"
     gene            complement(11397..12182)
                     /gene="cdaA"
                     /locus_tag="PLM68_03080"
     CDS             complement(11397..12182)
                     /gene="cdaA"
                     /locus_tag="PLM68_03080"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT54381.1"
                     /translation="MPDFLRLDIVDIIDIILVGLLIFQVYKIIRGTAAVNIFLGIIFL
                     YFIWMVVRAFHMELLTTILDKVISVGVLALIVVFQQEIRRFLLRLGTRYTKEGKRRGL
                     MESLFRPGQEALSKNDIEEIIHACSRMSERKTGALIALERFASLGFIAETGDRIDARL
                     HSRLIENVFVKDSPLHDGGMVISNGLIQAARCTFPMSESQGIPPYFGMRHRAGVGLSE
                     QTDAFVIVISEATGTIATMQDGVIKTLSGVAELRFDLETAFNR"
     gene            complement(12202..12876)
                     /locus_tag="PLM68_03085"
     CDS             complement(12202..12876)
                     /locus_tag="PLM68_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54382.1"
                     /translation="MRGGVMDMCFPGTCRVCGRILILQEKMLCLQCLYDLPRTYHWLF
                     PGHAMTGPFSCMAACTGATSFLFFREASPYRKLLHRFKYCGEKEIGLYMGFLYGKELS
                     GLSRSEVNPCIIPVPMSSGKRRKRGYNQAEWLAAGIALATGWTIDVSALKRIREKTSQ
                     TVYDREGRRENMKNSFAAKKVPYPEILLVDDIITTGATMESCALAILENNPGIRIGFA
                     SLAYVE"
     gene            12913..14355
                     /locus_tag="PLM68_03090"
     CDS             12913..14355
                     /locus_tag="PLM68_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT54383.1"
                     /translation="MKRRLLLSVLILVFLAGCNNPQLYLHNVFKSIDLEVRTHSQAYT
                     MLTDICERFGSRMAGTESAEQAEEFVSLLFRSYGFEDVFFQPFTFNQWQRGDVRLRID
                     TAGTYVDLPALALAFTPSGNVNASIVDAGNGLINDYNDLDVTDKIVLIHLGLLPGTPS
                     NIKNIHRARKLSLAIAQGAAGCIAVSPVPGGLPVTGSATLDGEMIRIPAVSISYEEGE
                     KLRASLSRRDARARIVTTNSCQPAYARNIVARMEGYEYPEETIVVGAHLDTWDISPGA
                     LDNASGAVSVLEIARILKALDLKTRRSVEFVLFMAEEEGHYGSELYVREARQRNLLDG
                     IRYMFNHDMTINTYGFNLMGHHESEDFFKKTGALIALTDSAFTDTMEHITYMGSDHAP
                     FLMEGITTFTPLCRYEAFHTYHTNADEPQYINPEMINSNVKASAMMVYALANAPELPA
                     SRMSQEQLRTYLIDNQLQEELVISGDWAWD"
     gene            14379..15638
                     /locus_tag="PLM68_03095"
     CDS             14379..15638
                     /locus_tag="PLM68_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54384.1"
                     /translation="MKRLSIICTLICSLFLTGCTPHDAVDDITPDYFVEELSSWDFTI
                     KDAVSALLQAMELDETDPTVSKIMDLLECYLAKEIHGVAISYRTFDPLGNPVLGTGAF
                     FYPRDLDPRGIVEVPPIAYLDRGASAAIYLGRKRFFEEMFPVMLNYITINPDLIGVLY
                     TQDLPRPFLHDANSGLVAYHMRKAVEEYLLITENYRLGKSSSVVGYSLGGASAMSIAK
                     YYTTHPTGITVDQVFTGGGVYDGLEAFRAYARTGKSDYLAIPSVIIAMDTYYELGLDY
                     SKIFANGMENPVNSPDPALGGDGYAYWFDSTHSSGSIHRRWGSDLRNYMHEDFFHDQP
                     QGEFLKLKACMELNSLVYNWEPKPALDIRVIHSGEDNLIPVECADLLYKVYREKGCSI
                     QYIRTTGDHYQAGSEFMLTAMLYFLLK"
     gene            15663..>15972
                     /locus_tag="PLM68_03100"
     CDS             15663..>15972
                     /locus_tag="PLM68_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54385.1"
                     /translation="MKTKSLSLSVIFLASCCWWGCTTQDSITSFTPHYLVEELGVWDF
                     TAGYASEAALDLVDVMDFDFPYDALETLAKIFPDGYDRPIRGVSISYRTIDPFGNPVI
                     A"
BASE COUNT         3914 a         3895 c         3842 g         4321 t
ORIGIN      
        1 ggctgcaggt tcgtgcgata catagaaacc gctgccaacc tggggagttc ctttatagga
       61 agttctcttg cctacaatgg taagaagatc accggcctta agcccggttt ctgtaaccag
      121 tgtctggaat agtccctggg cgccattcca gcctgaaagc aggccataca cgtaaacctg
      181 gccggtttca tcggaaatat tgaaattgcc gtatttgccg gcacctgaca aatcaccggt
      241 agtgccggtc aggcgataat aggtggcgtc ctcggcagca gccaggaaag tagctacagt
      301 ggcatctacc accggaatat gcgtctcgac cttggcgttt acaccctgcg gggtaccctt
      361 gtatgaaccg cggggagtaa tcagggtaac aatatcaccc accttgatgc ccatgttctt
      421 ccattgtttt gctttacctt ctgcgtcaag cactccatat atataaactt cgccggtggc
      481 gtcttttatg tatatgttgc cataatcgct tccggtgtct tttgtgataa tgccggaaat
      541 acggtattgg gtggcatcct cagctgctgc aataaaatgg gctaccgtag cgtcgatgat
      601 ggcgccggtc tgtttcacag taatcgtgat agtggaagaa ttgctgtttg tggcagatgt
      661 catggaaatg gtagcggtgc ggtcccctcc ggcattgggt tgggctacaa atgcgacgcg
      721 tgtggaatca tcggctccct tgatcatgga tttaacagaa agccaatcgg catcgtattc
      781 gatttccagt ccgttgcctt tgtataacac agccacttca aaagtgccgc cttccttggg
      841 tacctggacg gcatccacag cagacttgat gagcgagggg gtaatcttgt caacggtcac
      901 atcaacgagc tctatagtgg aaccgtaggc aaggcgtggc ccgtgcacca tcacgcggtc
      961 tcccactgac agacccaggc tcaggaaatt tttcgtattg cccttggcat ccagggtccc
     1021 gtagatgtac agggtccctg tcccgtcatt cagataccag ttgccataga cggtgttggc
     1081 aatagaagta acctgtcctt caacccagta aatcttgccg tcaacgcctt tatcaatcac
     1141 atctttacat gtggatatca caatttcctg ggggccggca atctgcttta cggtcaggac
     1201 ctgtaccttg ccggcacagt tgacataaac ttcggatgat ttgtcagaat cagttgctgc
     1261 agtagtaaag gttaccttaa taccttccgg ggaagcgctt ccgctggcag ggcttactgt
     1321 aagccatgtg ggtatattgg tgatatccca gctatcggta gcggtcaggg tgattgttct
     1381 tgtaccccct tctttagcaa aggcaatata ggacatgtct acttttacct cgtccaggga
     1441 aggtatgtca atttcctctt tacaacctgt aagcaaggct gcaaatgcaa aaaacgatag
     1501 taataaatat ttaagtttca taaggaaagt tttagctgat tagttaaaaa gatacttaat
     1561 accaacctgc agagaccagc attggccaat tgcatgactg taatcccagg ttctggcacc
     1621 tgcccctact cttgtcttga atacaggaac gccctgttcg ttgattttat ccagttgaag
     1681 gatacgtccg ctcattgcct cggtagagag gatctggtga acaccccagg tgctgttaaa
     1741 caggttacca atgttctgaa aatcaacgtt tagctgaagg gcattcctgg ttttgccaat
     1801 tctgaccaca aaatcgtgtg cataacggaa gtcaaagcgg tggacccagg gtgaatagac
     1861 actgtatgct tctgcgtact ttcctttatt ctttgacagg taagcatcct ggtcagcata
     1921 agcccagtaa cgttcacggt cgctttgaga agcgaagagg atctctgaat catccttggg
     1981 aatgtacatt acatcatatg caatgttgtc cccgttaaag tcgccgttgt acagatacgt
     2041 gtagttggaa ccgccgcgat agccttcata taacagggag aagtggttgt tgctcctgtc
     2101 tttataggaa accgaagcaa taatacggtc aggagttaca tattgggaat tgctgagtcc
     2161 ggcaaagttg ggaccttcca ctgtgggaat atagaggtag gcagactccg cattggaccc
     2221 gggcatgcct gtgatctgtt tcatgaccgt atgggtgtag gaggccatga tatgaaggtt
     2281 ctcaaccggg gtcatgttca acgtgaagct gcctgtccag ccgtatcctt tcttggtgtt
     2341 ggtaagcaca aaagcatcgg tgctggtgta tttgtaattg gcaggataga tgtgacgcct
     2401 gtcgggtccg ttcaattgtg tgaatccggc gttttccttg atgttccagt tcaacagctg
     2461 gattccgttg attgttttgt tgaaaatata ctcaccgctt atggagaagg ggaacgaggt
     2521 ggggaagttg taatccagcg caatggagga tttccatacc tggggcatcc tgaagtttgg
     2581 atcaataccg ttgatctggg agggtttcac cccgtcttcg ggagaaatgg tttttgggaa
     2641 ttttgtggga tcaaggctgt ggagtttctc aatcagggcg tccacatcgg tgatcatgtc
     2701 accctggaat gcggccagaa gagcatcggg attggctatg gcgcccgaat attttgtgga
     2761 aattgccaca atgttctgta ccatacccga gttggtaggc atattggtga agaacacaag
     2821 cggaagacgt cccgagaaca aaccggaacc tccgcggatt tttagggagc ggttcccgaa
     2881 tacgtcccag ttgaatccga tacgcggaga aacctggagt ttaggtttgg gccatttgcc
     2941 ggtatctaca ctcttgccat catattcaat cgcataaatg gcattgtttc gcatcaggtc
     3001 cttattgttg aaaatgattt catccagacg gacaccgtaa ttgagtttaa agttgcgtcc
     3061 tatgctccat tcgtcctgca gatacagtcc gaattggtta aagcgcacgc gggcagccgg
     3121 gttttgttcg ccgtcataac cataggtcag ggctaccgtt tcaggaacag caccgctcag
     3181 gaagtcatcc agactgcggt agcggtagta acccgttccg tttctcatat aagagttgtc
     3241 ggccatctgg tattcgaaac tcaggccggc cgtaattttg tggttgccct tatataaggt
     3301 aatatcgtcc ttggcgttga tcacggtgtt gtgaaccccg ttgttccagg taaacagctc
     3361 gtaaccggcc gacatgtaag gaacaatagc ctggctgatc tttccgttgt ctaccgtata
     3421 tccttccaga atgtcaatga aggggaattt ggcagaattg gatccgcgga tatcgtccaa
     3481 ctgcgaatag gtaacaagca actggttgga aatattgtct ccaaaacggc tgttaaggtc
     3541 cacagaaacg gaatttacaa tgttatccat agagtacatg gaattggcaa aagccatgga
     3601 atactgtccc aggcggcttt ccgtggtacg ttgtatacag ttggaagaag aggcattggt
     3661 gttgttccag cccctgttca acgtgtagtt gtaacgaatg gccaggttgt gattctggct
     3721 gatattccag tccaggcggg ccaatatctt catattgctt tcattggccg gataatcggt
     3781 ataggaaccg gtatcatagc cgtatttgct cataaggtaa tcagatgctc tcttcatatc
     3841 cgccatggtg gtgcgggaaa tgtaaaggtc ggtattggca acgccgtcaa cggaaggtct
     3901 ccagcggttc acgatggtgg gtgtctgggt gtattcaaag ttgacaaaga agaacagttt
     3961 gtttttgaca atcggaccac ccagcgtggc accgtaagtg gtattgcggt caatgcccct
     4021 atcgccaagc tctaccccat caactgtgtt gccccgcata ttttcattgc ggtgatagat
     4081 ataggcactt cctctcaggg tgttggtacc ggattttgtg atggcgttga ctccgccgcc
     4141 aatgaagttg gtctggcgga catcaaaagg tgcgatcacc acttgaactt cttcaatggc
     4201 atctatggaa atgggggtgc caccgccggg aagactggaa ctcagaccga agttgttgtt
     4261 gaagttggca ccgtcaaccg tgaagttgga ggaacggccg tcacctcctg aaaagctcat
     4321 tccgcttccc ccgtaagggg aaagtttggt gatgtcagtt atggtcctgc taatggtagg
     4381 cagagtctgg atgttttggt tggaaatatt ggtggaagag ccaaatttct ctcccgcaaa
     4441 agcagaggat gcagatgcta cgatcacaat atcttccagt tggaaagtct cctccttgag
     4501 ttcagcactt aagttgtaca gatctccaag ttgaagcgta atgtccgtga aactcatcgt
     4561 ctgatagccg atatatgaaa tttcgacttt atagggccct ccgggacgca tcccctgaat
     4621 ggtgtatcta ccatcgttat tggtcacggc agcatacatg gttccggatg gcacgtgagt
     4681 ggccaccagg gtaactcctg ctgctgctgc cccggcctcg tcggtgatcc tgccacccag
     4741 ggacgaggtg gttacctgtg cgtaagctcc cacacataga aacaaaaacg ccacaagagc
     4801 gagaaaacgt tgaattactt ttttcataga tttttattta ggttattata agttgccatt
     4861 atcttgagca ttgaaaacac ggtgcaaaag taggacaaat catcaggata ataaataaaa
     4921 tacttatcaa attcatcaat tcagacccca atcacctggt gtgcaaacct tctcaatttc
     4981 cttttcgatg ttatccggta agttgtggaa gaaatcaaaa cctgtcagat cttccatgtc
     5041 gtctacggtc ttggcatcgg atgccacagg atatgaggcg gtgtaggcac ggttctcata
     5101 ccagaagcct atggcagaat atcccccgtt ttctgcctga ccagctttgt acttcaacaa
     5161 caccttgaaa tacaactttg gaacagccac ctggttgcca tcgttgtcct tcacataatc
     5221 aatactatca tcctcccggg tctggatcat ggctccggtc actacgtata acgtgtcgca
     5281 agcaagggac catccccgta tgtaggtttc caggttgccc cacaggttct ggttgaggga
     5341 tgcatcctgg gcagctatgt tggtaaagta aaaggtgctt tcatttatct cctgcgagga
     5401 aagccggtct gccgatggca actggtgtcc cctgtcccag ctggaccctc caaaagaata
     5461 aaacaaaacg ggttggtatc tcttgggcac tttaggattg taattccagg catcggaccg
     5521 gctaccgctt cccatgatat cgcggtacag cgggtaggct acccaatgag aaaggcggtt
     5581 ttgcgtatcg taccagaaag agtaattgcg tacacttttt cctctcagcg aggtcatatg
     5641 ggatatatag gcaaaattct tttcttcctg cattcccgac agatcgggca attccagcca
     5701 cgaccgcaca ggatccgaaa tcaaagcctc aaaaggactc tgtgttatgt gcaaggtcag
     5761 ggtcttttgt tcgatagagg tgttggttgt ggaaaaaaca atattcgcct cacggggctt
     5821 ggtctcagag ggattttggg tatatgttac cgtaaccggg gcagtcttgc ttccggatgc
     5881 aggtgacacg gcaaaattct ccggatcaga ggagataaca gtccagtcaa cgttgccggc
     5941 cacagatacc gtaaattgtc cttcttctcc cggaatttcc ttttcagaag tatacagggt
     6001 aaatgtagga gatgtgtaga aacgctcacc cgtaagacct tcccaatccg aaactctggc
     6061 aaaggaaatc tgatattttt tatcatatac gctggcaatg cctttgatca cgccacagcc
     6121 tgaagggaca atcacatcct tgaatgagga ataccgggag gagaaaatat caaatgtctc
     6181 ttttgtctct gctatcatgc ctatggaggt atgggctgat tccatgacaa atgtcttgcc
     6241 caggtcttca tccctcacct gcactttggg tatggctacg tacatggact catattgtcc
     6301 cgtaatgagt tgtgcggctg atatttcttt tacctcaagc gtttctgtgc ctgtctttgt
     6361 aatcttggcc agaggcaggc cgttgacctg gagggggcca ttgttgtata ccgataaggt
     6421 ctggccttgc agctgaattt ccactttgtc cccctgcttg aagtctgtgt tgttttctgc
     6481 acaaaagagt tggattcccg catttttgtc acttacaaca atggctttca tagatgtgta
     6541 attgttcagc ccctcgttgt ctgcatgcag atagttgctt atgactgtgg cttttacaat
     6601 taccttatcc gtcactttga catcggtccc cttgtatagc gcacgaagct gggaaatgga
     6661 ccagtatagg gtttcctctt ctttctgggt aatggtgatg cgcacttcct tctcggctga
     6721 gtagaccttg atggcgcctg tacggtcggc ccctgtattg acctgcaccg aaagagtcag
     6781 ggttgcgtta ccctggccgc ttcctggcgt aacggatagc cagttgcttc cttccgtcac
     6841 cgccactgac cagctgcaat tcgcggacac ctgaactgcc tgctcacctg cggcagcagc
     6901 aaaagtaacc tgcgtcttat ccagagaaag tgattgagga acaggctttt ctccacaaga
     6961 aacaagaagg aatgccggaa gaataagcac tttgctgaat tgtctaaggt gcataagctt
     7021 tgctgttttt tcaggattat gtccttatag aatgccgaac aacaaaaata taaaattatc
     7081 aacggctaaa aattgtcatt tgtgttctat gagaatgaaa aaccatatac cgttgactat
     7141 gagagacata aattattaac aaagacctct tttttataaa caacattcat ttaaggtatt
     7201 tgattgaaaa gaatttttac ctttgtcggc caatagacat acttgtggac agatttgcct
     7261 gcttgcaacc actttaaaaa tgctaaaatg agaacacttt tctcttctga atcagtgtct
     7321 gaaggacacc ccgacaaaat cgccgaccaa attgccgatg ccatccttga tgaattttta
     7381 agactcgacc ccgagtcgaa agttgcctgt gaggtctttg tcaccacagg gcttgtaatc
     7441 atagggggag aggtcacttc caatgcctgg gtggatatcc gggacatagc gcgcaaagtt
     7501 atagctgaca ttggatacac cagggccgag tataaattcg actcagaatc ctgcggcatc
     7561 atttccctga ttgaaagcca gtctcccgac atataccagg gcgtggtcag ggaaaaaccc
     7621 gatgaacagg gggcgggaga ccagggcatg atgttcggat acgcctgtac cgagaccgaa
     7681 gagctgatgc cgcttcccat agtgctggct catattaccc tgatggaact ggctgccatc
     7741 cgcaaggaag gaaagcagat gacttacctc aggcccgatt caaaatgcca gtttaccata
     7801 gaatacgatg aaaagcagaa agctcagcgt attgacaccc tggttttgtc cactcaacat
     7861 gatccgtttg ccgaggagca cctgatgcac aggcagataa cagaggatgt ccttaacatt
     7921 ctcctgcccc gggtgataga caaggttcac ccctcgcgca aacatctctt tgaacacgat
     7981 ttgaaactac tggtcaatcc gaccgggaaa tttgtcatag gaggacccca cggagacacc
     8041 ggcctgaccg ggaggaaaat aatcgtcgac acgtatggtg gacgcggtgc ccacggtggc
     8101 ggcgcattct ccggaaagga ccccagcaag gttgaccgct ctgcctgtta tgccgcacgg
     8161 catattgcta aaaacctggt ggccgccggt gcggccgagg aggtgctggt acagcttgcc
     8221 tatgccattg gtgttgcgca gcctgtaagt gttttcgtta atactttcgg aacagcgcat
     8281 accaaaaagt ccgatgccga gatcgcacag atcattgcta aggtttatga cctgagaccc
     8341 gctcgcatca ttgaggaact caggctgaaa tacccgatat acaaagccac tgctgcctac
     8401 ggacatttcg gaagggaccc cttcagcaaa aacggaattg actacttttc atgggaaaag
     8461 ctcaattacg cagaagtatt caaatcaagg ctattctaag gcgtaaactt tccagttcct
     8521 tccttcacga cgaagtgtta tgaacgattc accgggttcc ttcaaacggg aaaactcaac
     8581 ccggtagggt aagcgaacag tatcgccctt aatcttttca ggtgcttcca ccgtaatatt
     8641 gatagccgaa agatagtcct tcacctcctg gtgctcctcc ggggtgagcg aatcaaatat
     8701 tgactgggat ttatcaagaa tccccatgat ttcaggcgcg gcataaacac gtgcctgctt
     8761 ataatcagaa gcaaaaaaag attgcaggaa atccagggca acctgctccg gttcctggcg
     8821 gcagctgcag gagctcatta cagccaggca tagcgtgatc cataaaattc ttttcatttc
     8881 aaatatacta cggtaattcc gggtcccccc ctttcaacgt gttcgtcctg gaaagaggtc
     8941 actcccggag cggttttcag gtaatttctg atctcggttc gcaaagcgcc tgtccctgtc
     9001 ccatgtaata tgtccacctg ttccatacct agcaccaggg cgtcgtccag aaaacgggtc
     9061 acttgctcca gggcttctcc ggccctaagt ccccgcacat ccagatgcgg gaaaaaatgc
     9121 aggcggcgtg cctgcaaatc atccggtatt accacctttg gactgccctg catgcgtgta
     9181 agagacctgt attcattctg tgatactctc tccagcacgg ttatctctgt ccaggtaacg
     9241 atctgaccca ttcctatata agctttattg ccttttatcc tgagtacttc gcccaccagg
     9301 gcaccatctt tggtccggac tttatccccg gccttgagag gacccttgtc ttcatcggat
     9361 tcctgtgctt tttgtcttgt ttttccttct ttcccggaaa gtttttcacg ctgttgcttt
     9421 cttttttcac ccgcttcttt tcgtttacgc aattgttcca tttttcgcag gattttctca
     9481 tcttccgcat cctgcgcctc ttgtgaaagt tctttcctga atgtttgcag gtcttcccgg
     9541 gcacttttcg ttttttccct ttccgcctgg gattccttga tttcccttat ggtgttttct
     9601 attttgcggt tggctgtttc aactatgcta agcgcctctt tacgggcttc atccagcatc
     9661 ttccttcgta acaggcgtat ttcctccagt tctgcctggt accttcccgt aatattttcc
     9721 agagtcttgt ctgtctgact gatgcgggcc actttttcct cccatttcct gcggttgcgt
     9781 gcaatactgc ggatataccg ctctgtttcc acaaatccgg caccggcttt ttcctgtgct
     9841 tcctggatca gatcggccgg gaggcccatt ttgcgggcca gttcaaatgc aaaggaatta
     9901 ccgggtaccc ctgtttccag tttgaacagc ggctggatgt tctgcacatc aaactgcatg
     9961 gcgccgttga ggactccctt gctggtggtg gcaaaaaatt tcagattcgt gtaatgggtg
    10021 gttatcacgc catagctccc gcacagctcc catctccgga gcaaagtttc tgcaatggca
    10081 cccccggccg caggttctgt tcctgccccg aattcatcta tcagtatcag ggacctttca
    10141 tctgcctgct ccaggatctc cttcatgttc agcaagtgtg aactgtaggt gctcaaatcg
    10201 ttatccaggg actgctcatc accaatatcc agaaatattg accggaaaat ggggaattcc
    10261 gaagtttctg cagccgggac tagcagaccg cattgaaaca tgtattgcag caaacccacc
    10321 gtcttcagac atacagattt gcctcctgcg ttgggtcccg agataagcag gatccttttt
    10381 gccgcatcca gtcttaaggt aagcggcact atctgctttc cttcacgttg cagggccgtt
    10441 tccagaaaag gatgacgggc ccgtatcagg tacaaaccgg tatcttcatt cagtataggt
    10501 ttgcctgcct gcattttcag ggccatcctg gctttggctc gtataaaatc aatagtcccc
    10561 aggaaacggg cctgctctgt cagttccgga acatatggcc ggagaaaacc ggcaaattcc
    10621 tgcaaaatcc tgatgatttc gcgttgctgt gcaaattcaa gttcccggat ctggttgttc
    10681 aggttgacca cctccatggg ttctatgaag acggttttgc cagtggccga ttcatctatg
    10741 atgtaccctt gtattttctt tttatttgcc gccgggacag gaatcagcat acgaccgtcg
    10801 cgtatggagg ccgaggcatc gggatcggtg attccctggt cgcgcgattc cttcagtatg
    10861 ctttgaattt tctgaaccac cagctgttcc ttgtttttca aagatttgcg gatcccggcc
    10921 agttcagggg aagcattgtc gcgaatttcg tcggatttat ccaatatgtg ttcaatcttt
    10981 tggacaatgg caggaaagaa tgaaatgggc cggcacagat ctttcagtgt ccggtaactt
    11041 tcctggcggg tgtgttcaaa aaaatgcaat aaggcgcgta cggtgccgat agctctttgc
    11101 aggcggacaa gatcaggaac aaggaggtag taagcaggat tcgactccat ctgtttcagg
    11161 aagtagaggg tgtcttcgta tccgtcttcc gggaactgtt gttcaaacag gcatatttcc
    11221 cgcatttcgc cggtcctttc cagggatgtt tccagcgtgt cgaaatcagt gaggaaaacc
    11281 atatccttta ccagttcacg cccgaaactg ctcatgcaca gttgtgtgag ctgttcccgt
    11341 atcctgtcaa aacctatttt ttgctcaaac tggtccggat agatcataat agctgcctac
    11401 ctgttaaagg ccgtttccag atcaaagcgc aactcggcca ctccgctcag tgtcttgatt
    11461 acaccatcct gcatggtggc aatagtccct gttgcctcag atatgacaat gacaaaagcg
    11521 tctgtttgct cgctaaggcc aaccccggcc cggtgtctca tgccaaaata tgggggtatt
    11581 ccctggcttt cagacatggg aaaagtgcat cgggcagcct ggatcagacc attggaaatg
    11641 accatgccac cgtcgtgaag cgggctgtct ttgacaaata cattctctat cagccggctg
    11701 tgaagccttg cgtcgatcct gtctcctgtc tcggcaataa aaccaagcga ggcgaaccgt
    11761 tccagggcta tcagagcacc tgtttttcgc tcagacatcc ggctgcatgc atggatgatt
    11821 tcttctatat cattcttgga taaagcctcc tggccgggac ggaaaaggga ttccatcagc
    11881 ccgcggcgtt ttccttcttt agtatacctt gttcccagcc gaaggagaaa acgtctgatc
    11941 tcctgctgga acaccacaat cagggccaga acacccacgc ttatgacttt atccaggatg
    12001 gtggtcagca gttccatatg aaatgccctg actaccatcc agatgaaata caggaaaatg
    12061 atacctaaaa aaatattgac ggcggctgtt ccccgtataa tcttatatac ctggaagatt
    12121 aataaaccca ctagaataat atcaatgata tcaacgatat ccaggcgtaa aaaatcaggc
    12181 atagtgcgca aatatacgtt tttattctac atatgcgagc gaggcaaaac cgatcctgat
    12241 acctggatta ttttccagga tggcgagggc gcaactttcc atggtcgccc cggttgtaat
    12301 tatgtcatcc accagcagga tctcggggta tggtactttt tttgccgcaa aggagttttt
    12361 catgttttcg cggcgccctt ccctgtcata tacggtctgg cttgtttttt ccctgatcct
    12421 ttttaaagct gaaacatcta tggtccagcc agttgcaaga gctatccctg cagccagcca
    12481 ctcggcctgg ttgtaacctc ttttcctgcg tttaccggat gacatcggaa ccggaataat
    12541 gcatggattt acctcgctcc tgctaagccc ggacaactcc tttccataaa gaaaacccat
    12601 atacagaccg atttcctttt ccccgcagta tttgaaacgg tgcagcaact tccggtaagg
    12661 acttgcctcc ctgaaaaaaa gaaatgaagt ggcaccggta catgcggcca tgcagctgaa
    12721 aggtcctgtc atggcatgac cgggaaataa ccaatggtat gttcttggaa ggtcgtacag
    12781 gcattgcagg cacaacatct tttcctgcaa aatcaaaatc ctgccgcata ccctgcaggt
    12841 tccgggaaaa cacatatcca tcacaccacc ccgcatggac caaatttata aaatattttt
    12901 atatttgctg acatgaagag gaggttgctt ttaagcgttt taatacttgt gttcctggcc
    12961 ggatgtaata atccacaact ttatcttcat aacgttttca aaagcattga cctggaagtc
    13021 aggacccatt cacaagccta taccatgttg actgatattt gtgaacgctt tggttcacgg
    13081 atggctggaa ccgaatctgc tgagcaggca gaggaatttg tctccctgtt gttccgttcc
    13141 tatggatttg aagatgtatt cttccagccg tttaccttca atcagtggca aagaggggat
    13201 gtgagactaa ggattgacac ggcgggtact tatgtggatt tacctgccct ggccctggca
    13261 tttactcctt caggaaacgt aaatgcgtcc attgtagatg cggggaacgg acttatcaat
    13321 gattacaatg atcttgatgt gacagacaag attgtgctta tacacctggg gctgctcccg
    13381 ggcacccctt cgaacataaa aaacatacac cgcgcccgga aattgtcact ggccatagca
    13441 caaggcgcag ccggctgcat tgctgtcagt cctgttccgg gaggccttcc tgttaccggt
    13501 tcggcaactc ttgacgggga aatgatcagg ataccggcag taagcatttc ctatgaagag
    13561 ggcgaaaaat taagggccag cctatcccgc agggatgcca gggcccgtat tgtgaccacc
    13621 aattcctgcc aacctgccta tgccagaaat attgtggcaa gaatggaggg ctatgagtat
    13681 ccggaagaaa ccattgtcgt gggggcccac ctggatacct gggacatctc tccgggtgcc
    13741 ctggataacg cttccggagc tgtctctgtc ctggaaattg ccaggatatt gaaagccctg
    13801 gacctgaaaa cacgacggtc tgtggaattt gtgcttttca tggccgaaga ggagggtcat
    13861 tacggttcag agctgtacgt aagagaagcc cggcaaagaa acctgctgga cggtatccgt
    13921 tatatgttca accacgacat gaccattaac acatatggtt ttaacctgat gggacatcac
    13981 gaatcggaag atttcttcaa aaagaccggt gctctcattg ccctgactga ttctgccttt
    14041 accgatacca tggagcatat tacctatatg ggtagcgacc atgctccatt tcttatggaa
    14101 ggaatcacca cattcacacc tttatgccgg tacgaggctt ttcatacata ccataccaat
    14161 gctgatgagc cgcaatacat aaatcctgaa atgataaaca gcaacgtgaa ggcctctgcc
    14221 atgatggtat acgcattagc caacgcacct gaacttcctg cctcacgcat gagccaggag
    14281 cagttgcgta catacctgat tgataatcag ctacaggaag agctggtcat ttcgggagat
    14341 tgggcctggg attgatttat taatataata ctttaataat gaaacgttta tctattatat
    14401 gcacattaat ttgttcatta ttcttgacag gttgtacacc gcatgatgca gtggatgata
    14461 ttaccccgga ttattttgtt gaagaactca gttcctggga ttttacgatc aaagatgccg
    14521 tttcggctct gcttcaggct atggaacttg atgaaaccga tccaactgta agcaaaataa
    14581 tggacctcct tgagtgttat cttgccaagg aaatccacgg tgttgccatt tcctacagaa
    14641 cgtttgatcc tttagggaat ccggttcttg gcacgggtgc gtttttctat ccccgggacc
    14701 tggaccccag gggcattgtg gaagttccgc ccatcgcata cctggaccgt ggagcaagcg
    14761 cagccatcta tttggggaga aaacgtttct ttgaagaaat gttcccggtc atgctcaact
    14821 acattaccat caatccggac cttattggag tgctctacac ccaggatttg ccccgccctt
    14881 tcctgcacga tgccaattcc ggcctggtag cataccacat gcgcaaagcc gttgaggaat
    14941 acttgctgat aaccgaaaat taccggcttg ggaagagtag ttctgtcgtg ggctattccc
    15001 tgggaggagc ttctgccatg tcgatagcca aatattacac cacacatcct accgggatca
    15061 ccgttgacca ggtattcacc ggcggcggtg tatatgacgg gctggaggct ttcagggctt
    15121 atgcacgaac agggaaaagt gactacctgg ctattcccag tgtgatcatt gccatggata
    15181 cctattatga actgggcctg gattacagca aaatctttgc caacggaatg gaaaatcctg
    15241 tcaactcacc ggatcccgca ctgggcggtg atggttacgc ctattggttc gacagcacac
    15301 atagctcggg gagcattcat cgtcgttggg gaagtgactt gcgcaattat atgcatgagg
    15361 atttcttcca cgatcagcca cagggtgaat ttctcaaact caaagcctgt atggagctga
    15421 actctctggt ttacaactgg gaacctaaac ccgcactgga catccgcgtg atccacagcg
    15481 gtgaagacaa cctgatcccg gttgaatgtg ccgacctgct ctacaaggtt tacagggaaa
    15541 agggctgttc catccaatat atccggacta caggagacca ttaccaggca gggagtgaat
    15601 tcatgctgac agcaatgctt tactttttat tgaaatagtc atatgaccgt ttcctgggtt
    15661 ttatgaaaac aaagtcttta tccctaagtg tcatctttct tgcctcttgc tgctggtggg
    15721 gttgtaccac ccaggacagc atcacctctt tcaccccgca ctacctggtg gaagagctgg
    15781 gcgtatggga ttttacagca ggttatgcct ctgaagccgc tttggatctt gttgatgtga
    15841 tggactttga ttttccctat gacgcattgg aaacacttgc aaaaatattc cccgatggat
    15901 atgaccggcc catccgtggc gtttccatat cctacaggac catcgaccct tttggcaatc
    15961 ccgtcattgc aa
//
LOCUS       DAOSOF010000031        15934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3703_length_15934_cov_5.126834, whole genome shotgun sequence.
ACCESSION   DAOSOF010000031 DAOSOF010000000
VERSION     DAOSOF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_15934_cov_5.126834"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLM68_03105"
     CDS             complement(<1..225)
                     /locus_tag="PLM68_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOT54386.1"
                     /translation="MKITITFAALLACLPMVAQEKAVIDLQPQKDTWRIEKEIYGHFA
                     EHLGTCIYGGLWVGPDSPIPNTQGYRNDVLD"
     gene            complement(308..2686)
                     /locus_tag="PLM68_03110"
     CDS             complement(308..2686)
                     /locus_tag="PLM68_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HOT54387.1"
                     /translation="MKKYLKVCLLAALLLPLAGCGDSKQPTVYIDGVPQLNPDNIEEI
                     IQAMTTDEKVSLLVGTGMAGFTGDNPVVGETRNLVPGAAGTTWPIPRLGIPAIVLADG
                     PAGLRIAPLREGDENTYYCTAFPIATQLASSWDLELVELVGQNMGAEVLEYGCDIILG
                     PGMNLHRNPLCGRNFEYYSEDPFVTGKMAAAMVRGIQSNGVGTSVKHFAVNNQETNRM
                     GNDARLGTRALRELYLKGFEIVVKESNPRTIMSSYNKINGTYASQSRDFLTTITRDEW
                     GFQGTVITDWFGGIDAPAQIHAGNDLLMPGRLNQKEDILKALEDGSLQEEFVDTSVRR
                     ILELIVVSPRFKGYPYSNKPDLEVHAQVTRQAGAEGMVLLKNSESLLPLAENVRKVAA
                     FGNTSYAFIAGGTGSGDVNEAYTVSLVEGLENAGFELHQDLKKEYLDYRGKEIEKREK
                     TRSRDMLSAFMPKKPLEEYLPSEKMIRRMAQENDIAFITIGRNSGEFLDRRLEDDFLL
                     TLAEKEMLGKVSEIFHQEGKKVVVILNIGGVIETSLWKDLPDAILLAWQGGQEGGNAV
                     ADVLSGKVTPSGKLTMTFPIAWEDHPSSANFPTGNEYQTGAPLMALMQNGTTEPERDP
                     IANVDYTEYKEDIFVGYRAFEHNGTEVSYPFGYGLSYTTFQYGEPEISVHRGIYTVSL
                     DVTNTGSFPGKEAVQLYVAAPGKDMVKPGKELKAFAKTGLLGPGKTERVEMVFTTYDL
                     ASFDQERSGWVTEPGTYKIRLGASSDDIRSEASISVKKEIFKAVNNVLTPQI"
     gene            complement(2712..3896)
                     /locus_tag="PLM68_03115"
     CDS             complement(2712..3896)
                     /locus_tag="PLM68_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT54388.1"
                     /translation="MSKKIVTLTLACLLCHSVLTAQQALFWGGPQITSPEVNPDNSVT
                     FRLLAPDAKEVKLTGDFLPPQGWIPGSEPMTRDEKGVWSYTTKPLESDLYGYALLVDG
                     LRMMDPNNVHQIRDVATVTNIVITPGGQGDLYSVQNVPHGTVTRTWYESPSNKYVQKR
                     RITIYTPPGYEQGKGKYPVFYLLHGMGGDEEAWINLGRTSQIMDNLIAAGKAEPMIVV
                     MTNGNVVQEAAPGESSLGLYKPTMQLDGTMDGQFEVSFADVIRFVESKYRVIPKKNAR
                     AIAGLSMGGYHTLHISRYYPNTFGYIGLFSAAIMPDQSHADSPVYRDVEGTLKKQHEN
                     GYSLYWIGIGKEDFLYKANTEYRELLDRINMEYVYRESEGGHTWTNWRVYLSEFAPLL
                     FK"
     gene            complement(3940..5514)
                     /locus_tag="PLM68_03120"
     CDS             complement(3940..5514)
                     /locus_tag="PLM68_03120"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54389.1"
                     /translation="MKKYVITLIFGLLLLNSCAERLNIEQWGVLSEDTFYQTDEQATE
                     AITAVYSTWRGQYFNWFYLKNMLSDDTYCGGGGRGDNSNFEMINEYRFSTASTVVSGA
                     FSGFYSIIYMSNLIVDRVKPDTDIKARVVGEAKAARAWAMTELISLWGAVPFADHVLG
                     ADEYQLPNGDIPTMWAWVEQNLTEAAAVLPSKSGINGQQAIGARLTKEAALAFLGKAQ
                     IFQEKYAEAATSLKSVINSNKYDLVADFSQLLRSANDFGPENIFESNAINDPDNAWSQ
                     GTMLFSLMINWRSDHLTGMPEGIWNTGWGFFNPTKSLYEAFVAMEGPEGYRLTNTMKT
                     REQMGGGWAFNWETFQMVYVPIQITTPLYGNEGFMQWKHRFPSDEVVTNSWGYSSYNN
                     FRWMRYAEVLLLAAEACLLSGDQASALNYINQVRARAHLESLGSLTLEDIKKEKRLEL
                     CFECTRYQDLVRWGDASSVLAQQGKIIPALHNDGKVTVAVTNTDFGFKSGKHELLPFP
                     EHEMNVNKNLVQNPGW"
     gene            complement(5527..8619)
                     /locus_tag="PLM68_03125"
     CDS             complement(5527..8619)
                     /locus_tag="PLM68_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54390.1"
                     /translation="MKKMIYQPVTRLVLLVFSLLLILPASVAAQQAQISISGTVVDQS
                     GLALPGAYVAVVGLQRGVSTDQEGKFVISVPQNATISVSFVGYKTVEIPVGDRRVFEI
                     TLEEDALLLEDVVVIGYGVQRKSDVTGAIAKINTTDIENRSVARVEEALQGKTAGVQI
                     ITTSGAPGKGMDIRVRGISSNSGSVDPIILVDGLQVSDIGYLDPNNIESIEVLKDAAS
                     AAIYGAQAGNGAVLVTTRSGKRAGEGSFFYDFQYVLNDLAKIPEVMNASQYMDYMVEA
                     GTFTQAEFDQKYDGVTDTNWADVAFETSLMQRHSLGFQGGNERGNFFASINYLDQNGI
                     VAGDADVYHRLTGQINADYKIKKWLRIGVTTSLEKWDSRSVSESSEYGSLLAAVLAMD
                     PLTPNLYSPSQLPQFMKNNQNDPDWVALYNDYLKNESGDYYGVSQFVTSEQVHPMIMR
                     DATQSDSWGANVLGTVYADFTPIKGLTITSKLGYRLGYNNSKTYGGLYYANDMVYRSK
                     MTLSRSSGNNIYYQWENFANYDVELNSHRINFMAGISFAESRNDYTSAGTDKLTKDLP
                     LFRDISYSDPTANRTIGGGFGRSAKMSYFGRVSYSYLDKYLLQAVLRRDAFDASVLPV
                     EKRWGTFPSVSAGWVISKEGFMENNKVLTYLKLRASWGQNGNIGPLGGFRYRTSIASG
                     YTYPYYNEIAYNIGSAPTGLTNPDIRWETSEQIDIGIDLRMFNDRLTFTADFFSKETK
                     DLLVATTPPYETGVSSTTVNAGNVHNHGFEFDLGFTGRIGDFNYNIRGNLSTITNKVT
                     YLDPSISRISGGSYHTYSGLTAFEVGYPVWYMRGYQLDHIDSKTGDPVFVDNNGDGLI
                     NDDDKVMIGSALPDFTYGITLNMSWKGLDLTLFGTGSQGNDIFSLLNRVDRPQGNKLA
                     LFYQERWTSSNTNARYPRPGGGDQDKFWTSDANVFDGSYFKIKQIQLGYSLPHKWMRK
                     IAVNSLRFYVSLDDWFCFTTYPGMDPEAASYGSTYGMGLDKGAYPLSKKTMFGLNITF
                     "
     gene            complement(8653..10302)
                     /locus_tag="PLM68_03130"
     CDS             complement(8653..10302)
                     /locus_tag="PLM68_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HOT54391.1"
                     /translation="MKSTISIIMCLFVLVVTGCSCGTDSHMFINKDDTQVTASSRDAI
                     AETFTGKVAGYIENGLAIFKGIPYATAQRFELPQRPVPWEGIRSCRAYGPVCPQAIRT
                     GWQSDELAFAFNWDDGFPGEDCQRLNIWTPGLEDGKKRPVMVWLHGGGYSAGSGQELP
                     AYDGASLALSGDIVVVSLNHRLNILGFLDLSAFGEKYSMTGNLGILDIITALEWVRDN
                     IAHFGGDPKNVTLFGQSGGGGKVSTLLATSRASGLFHKAIVQSGSMISAMEPEYSRKI
                     GIEVVRELALQPSHVEQIKNIPYQTLLSAGEKAVAKIRKEAEADGFQPLIFGWAPTTD
                     GIFLKEQPFDHEAPKTAKNIPMIIGTTLHEFSVSTYMPDLRNIDLQKAKEILKDKYGD
                     KTDAFIEAVAAAYPGFRPADLFDIDTRFRQGALHQAEVKVRQKGAPVYMYLFSWESPV
                     LDGTLRAMHCMELPFVFNNIKKSRHMTGGGEEAFRLAEKMSRAWINFARSGNPNVTSL
                     PEWDPYDPGTGATMVFDNQCRMEYLHDRELISFIFEMNKIQ"
     gene            10519..14556
                     /locus_tag="PLM68_03135"
     CDS             10519..14556
                     /locus_tag="PLM68_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT54392.1"
                     /translation="MVTTIFFLSVLLCGYTNTLSSTEFADGFFPLSSSLSNQNIRCFG
                     EDKHGYIWIGTGRGLNRFNGYEFCQYLRSGDSTALVDNQLQYIFRDSQDRMWFLTVNG
                     LSLYGPDGHFTNYPVEGPNRNCIKILENSEGTLYLSLGTSLSVFDPKSEAFRHALNIE
                     FSGFPNTFHMDRLDRLWLVTPAKVFCYDSKTLELKASWETPGYIAYSHLRNNGELWLA
                     GEQMLSILDTRTGGYSSLPPGITGNRMLMNSRITYIQPYNRNSMLINTADKGLFLYLP
                     LTDILIHQSDKDFPFDVPDAEITTIYEDSRSNIFIGTYDKGFYIRNPFRQSFGGNTLL
                     TKKYNGKNIVSLDTDSSGNLWISARREGVSLYDRVKDSIIDLSDMIERVVPGFLNDPL
                     INIHVAPDNTVWLYHYTELIALKYTRGNIRLVKRFKSLPPVISVTSDKEGTLWVGTFS
                     HQIFFLEKGSLTMKSRDLFHPGFVYIPHILPLSNGSLFIPGFKQNPRILHPGDMSYEE
                     LDIMHLFPDLPLIPSALMEDSAGIIWMGTVGNGLIKYTPATGEIIPVRDLSCNDISDL
                     LEDNAGQVWLSTQYGLIRYDRTIDRYFSYTALDGISGNQFNERSAVRLPDNTLVFGGT
                     HGITHFNPLDIAQKRSVPLCFEYLKIHNKLVMPSEDGPIKRSLTETPEIVLDHTQNDF
                     SISFAALDYHNAAPRVEYSYRLSGYDKEDISAGQYREAYYSNLPAGTYTFTVHIKNHD
                     NTIAEVSRSLSLRIKSAPWFSWWAWLLYGLSGALILGSILALYMRIKRNKTRTRQIEM
                     EREREIRQNETNMKFFSNISHEFRTPLTMIAGPISTLANDKAISEENRQLVIIIQRSV
                     RRMLKLINQLLDFYKLENDTLKLRVRKTDIIEPVNKLVDIFRINASEKDITLRTKGLE
                     DTFLTWIDEDKIDKIIGNLLANAVKFSTPGKGRIEVTFDVIDSKDAVKSFDLDQSAGF
                     VSSWYVQLSVGDNGPGFDNKDLAKIFTRYYQMERQKTGFLSWGTGIGLYYSRRLVETH
                     HGFIKAQNKPDGGALLTFIIPVDEAAYDPSELDYSETPVCADPAVNTSGPCPKIMQGD
                     KENIQELPVILLVEDDTDVAHYLKMLLRPNYHVIHEFDGQTALNIMETTIPDLIISDI
                     VMPGIDGFTFCRKIKDNLSYSHIPVILLTAKTTTEEKVSGFESGADAYITKPFEPPYL
                     EAVIKAQLKNRENLHRLLEKTTKPPLEIDDKELLPHDKQFLKGLYELMEKELSNPELN
                     IQRMTETLQMSRTKFYYKVKGLTGLNPNVFFKKYKLNRAEELLSTGKYNISEVADLTG
                     FSTLSHFSVSFKKHFGSSPSEYNRK"
     gene            complement(14585..>15934)
                     /gene="ettA"
                     /locus_tag="PLM68_03140"
     CDS             complement(14585..>15934)
                     /gene="ettA"
                     /locus_tag="PLM68_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOT54393.1"
                     /translation="EVNMKFSEPMDDDQMNELIIRQGELTEKIDNAGAWDLDSKLQRA
                     MDALQCPPAGNSVKYLSGGERRRVALCRLLLSEPDVLLLDEPTNHLDTESVQWLEAHL
                     QQYKGTVIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTIRLAMEEKQES
                     KRRKTLERELEWVRMAPKARHAKSKARLGAYEKMLNQDSRQKEERLEIFIPDGPRLGD
                     KVIEATGVAKAYGEKLLFDNLNFRLPPAGIVGVIGPNGAGKTTLFRLIMGVEKPDAGE
                     FRVGETVRLAYVDQQHKQIDPDKTVYQTIAGNSEYIRLGNRDVNARAYVSRFNFSGAD
                     QEKLCGILSGGERNRLHLALTLKENANVLLLDEPTNDVDVNTIRALEEGLENFAGCAV
                     IVSHDRWFLDRIATHILAFEGDSGVYFFEGSYSEYEENRKRRLGTEIKPFKYKKLQD"
BASE COUNT         4080 a         3916 c         3539 g         4399 t
ORIGIN      
        1 atccagcaca tcgttacggt atccctgtgt gttgggaata ggggaatcgg ggccgaccca
       61 gagtcccccg taaatacatg tccccaggtg ctctgcaaaa tgtccgtaga tttctttttc
      121 tatgcgccat gtgtctttct gcggttgcag gtcaattacg gctttttcct gtgcaaccat
      181 tggcagacaa gccaacaatg ctgcaaaagt gattgtaatt ttcataatcc aaatttacaa
      241 tttaacagga aaaaaccacc ccgtacgagg tggttttcca taatataaac agtttattgg
      301 taaataatca gatctgtggg gttaacacat tgttaacggc tttgaaaatt tcctttttca
      361 cagaaattga ggcttcagat cggatgtcgt cagaagaagc tcccaaacgt atcttatagg
      421 tgccgggttc ggttacccaa cccgaacgtt cctggtcgaa ggaggcaaga tcatatgttg
      481 taaaaaccat ttccacccgt tcggtttttc cgggtcccaa caaccctgtt ttggcaaaag
      541 ctttcagttc tttcccgggt ttgaccatat ctttccctgg agcagccaca tagagttgta
      601 cggcttcttt tccgggaaag ctgcctgtgt tggtaacatc aagacttacc gtgtaaattc
      661 cccggtggac cgaaatttcc ggctctccgt attggaaggt tgtatacgaa agtccgtaac
      721 cgaaaggata ggatacttct gttccgttat gttcaaaagc acgatacccg acaaatatat
      781 cttctttgta ttcagtataa tcaacattgg ctatggggtc tctttccggt tcagtagtcc
      841 cgttttgcat gagtgccatc aggggagctc ctgtttggta ttcgttgcct gtgggaaagt
      901 tcgccgaaga gggatgatct tcccaggcaa tgggaaatgt catggtcaat ttacccgacg
      961 gggttacttt cccggaaagg acatcagcca ctgcattgcc gccttcctgt cctccctgcc
     1021 atgccagaag tatggcatcg ggcagatctt tccaaaggga ggtctcaatt acaccgccaa
     1081 tgttcaggat aacgaccact tttttcccct cctggtgaaa gatttcagac actttgccca
     1141 gcatttcctt ttcggccagg gtgagcagga aatcatcttc aagacgacgg tccaaaaatt
     1201 ctccggaatt acggcctatg gtaataaagg caatatcgtt ttcctgagcc attctccgga
     1261 tcattttttc agaaggaagg tattcttcca atggcttttt aggcataaat gcactcaaca
     1321 tatcccggga acgggtcttt tcgcgttttt caatttcttt gccgcgataa tccaggtatt
     1381 cttttttcag atcctgatgc agctcaaaac cggcattttc cagtccttcc accaaagaaa
     1441 ccgtatatgc ttcgtttaca tcaccactgc cagtcccccc tgcaataaaa gcataggagg
     1501 tgttcccgaa agcggctacc ttacgaacat tttcggccaa cggaagcagg gattctgagt
     1561 ttttcagcaa gaccatacct tcagctcctg cctgacgggt aacctgagca tggacttcca
     1621 gatccggttt gttgctgtaa ggatacccct tgaaacgggg agaaacgaca atcagttcca
     1681 atatgcgcct gacacttgta tctacaaatt cttcctgcag ggagccatcc tcaagggctt
     1741 tcaggatgtc ttctttctga ttgagccttc cgggcatcag cagatcattg cctgcatgta
     1801 tctgggcagg ggcatctata cctccgaacc aatccgttat gaccgtaccc tggaatcccc
     1861 attcgtcacg ggtaatggta gttaaaaaat cccggctttg cgaggcataa gtgccgttga
     1921 tcttgttata ggaagacatt atggtacggg gatttgattc tttgacaaca atctcaaatc
     1981 ctttgaggta caattcccgg agtgccctgg tgcccaaacg ggcatcattg cccatgcggt
     2041 tggtttcctg gttgttcacg gcaaaatgtt ttaccgaggt gcccaccccg ttggactgga
     2101 tcccccttac catggcagct gccatttttc ctgtaacaaa aggatcttct gaatagtact
     2161 caaaatttct gccgcataaa ggattccggt gcagattcat cccgggtccc aggataatgt
     2221 cgcatccgta ttccaatacc tcggcaccca tgttttggcc aaccagttcc accaactcaa
     2281 gatcccaaga agaagccagc tgggtggcaa tgggaaaggc tgtgcaataa taggtgtttt
     2341 catctccttc acgcagcggt gcgatgcgaa gtcctgcagg accgtctgca aggactatgg
     2401 ccgggatccc cagacgggga atgggccagg tcgttcctgc tgctcccgga accagattgc
     2461 gggtttcacc cacaacggga ttgtcccctg taaaaccggc catccccgtt cctacgagca
     2521 gggaaacttt ttcatcggtg gtcatggcct gaataatctc ttctatgttg tcgggattca
     2581 attgcggaac accgtcaata tatacggtag gctgtttact gtctccacag cctgccaacg
     2641 gcagcaataa ggctgcaagt aaacatactt tcaaatattt tttcatcctt ttaaattttg
     2701 gaaacaatta gctatttaaa cagcaaagga gcgaattcag aaaggtacac cctccagttg
     2761 gtccaggtat gtcccccttc gctttcccgg taaacatatt ccatgtttat acggtccagc
     2821 aattcacggt attccgtgtt tgctttatag agaaaatctt ccttccctat acctatccag
     2881 tacagactgt agccgttttc atgctgtttt ttcagtgtcc cttccacatc ccggtaaacg
     2941 gggctgtccg cgtggctttg atcaggcatg atggctgccg agaacagtcc tatatacccg
     3001 aacgtattgg gataataccg tgaaatatgc aacgtgtggt atccccccat ggacaatccg
     3061 gctatggccc gggcattttt cttgggaatg acccggtatt tcgattccac aaaacggatc
     3121 acatccgcaa atgaaacttc aaactgtccg tccatggtac cgtcaagttg catggtgggc
     3181 ttgtacaatc ccagggaaga ttcgcccggg gcggcttcct ggaccacatt cccgttggtc
     3241 attaccacga tcattggctc tgcttttcct gctgctatga ggttgtccat gatctgtgac
     3301 gttcttccca ggttgatcca ggcctcttcg tccccgccca tgccgtgcag caggtagaaa
     3361 acaggatatt tccctttgcc ttgttcatac cccggcggcg tataaatggt tatcctgcgt
     3421 ttctgtacat atttgttgga cggactttca taccaggtac gtgtcaccgt accgtgaggc
     3481 acattctgta ccgaatacag atcgccctgg ccccccggcg tgatcactat gttggtcaca
     3541 gtagccacat ccctaatctg gtgcacatta ttgggatcca tcatacgcag gccgtctacc
     3601 agtaaagcat acccgtaaag atccgattcc aaaggctttg tggtatagga ccatactcct
     3661 ttttcatccc tggtcatggg ctcgcttccg ggtatccatc cctgcggggg taaaaagtct
     3721 ccggtaagct taacttcttt ggcatcggga gccagaagac ggaaagtcac ggaattatcc
     3781 ggattcacct ctggagaggt tatttggggg cctccccaaa aaagagcctg ttgtgccgtt
     3841 agcacagagt ggcacaacag gcaggccagt gtcagagtta caattttctt actcataaat
     3901 acgttttttg aattttccgt gaatgtactg gtatttgtgt taccatccgg gattttgcac
     3961 caggtttttg tttacattca tttcatgctc ggggaaaggc agtaattcgt gtttcccgct
     4021 cttgaatcca aaatcggtat tggtcacggc aacggttact tttccgtcat tatgcaaggc
     4081 cggaattatc ttaccctgtt gcgccaacac tgatgacgca tcaccccatc gtaccaggtc
     4141 ctggtaacgg gtgcattcaa agcacagttc aagccgcttt tccttcttaa tgtcttccag
     4201 ggtcaggctt ccaagggatt ccaggtgagc tctggcgcga acctggttaa tatagttcag
     4261 ggcagaagcc tggtctccgg acaacaggca ggcttctgcg gcaagcaaca atacttcggc
     4321 ataacgcatc catcgaaaat tattatagct ggagtaaccc caggaattgg ttactacttc
     4381 atctgaagga aaacggtgtt tccattgcat gaatccctca ttgccataaa gaggggttgt
     4441 gatctgaata gggacataga ccatttgaaa tgtttcccag ttgaaagccc atccgccccc
     4501 catctgttca cgggttttca tggtattggt aagacggtat ccttcaggac cttccattgc
     4561 cacaaaagcc tcataaagcg atttggtggg attaaaaaat ccccatcccg tattccatat
     4621 cccttcaggc attcctgtaa gatgatctga acgccagttg atcatcagtg agaagagcat
     4681 ggttccctgg ctccaggcat tgtccggatc gtttatggca ttggattcaa aaatattttc
     4741 aggtccgaaa tcgtttgccg aacgcaacaa ttggctaaaa tcagctacca gatcatattt
     4801 gttggaattg atcaccgatt taaggctggt agcggcttcg gcatatttct cctggaagat
     4861 ctgggctttt cccaaaaagg ccagtgcagc ttccttagtc agccgtgccc caatggcttg
     4921 ctgcccgtta atcccgcttt ttgaggggag aacggctgca gcttctgtca ggttctgctc
     4981 tacccaggcc cacatggtgg gaatatcccc gttgggcaac tggtattcat cggcaccgag
     5041 cacatggtcg gcaaaaggta cagcccccca gagtgaaatg agctcggtca tggcccaagc
     5101 acgtgcagcc ttggcttcgc ctacgaccct ggctttaata tctgtatcag gtttcacacg
     5161 gtcgacgatc aggttggaca tgtaaattat actgtaaaat ccgctgaaag ccccgctaac
     5221 caccgtgctg gcagtgctga accggtattc gttgatcatt tcaaagttgc tgttgtctcc
     5281 acggccccca cctccacaat aagtgtcatc ggacaacatg tttttcaggt agaaccagtt
     5341 gaaatactga cccctccagg tgctatatac ggcagtaata gcttctgtag cctgttcatc
     5401 tgtctggtag aaagtatctt cagaaagaac cccccactgt tcaatattca ggcgttcagc
     5461 acatgaattg agtagcagca gtccaaaaat caaggtgata acatattttt tcatggctgt
     5521 attcttttaa aaagtgatgt ttaaaccgaa catagtcttc ttggaaaggg gatatgcccc
     5581 tttatccagg cccataccat aagtggaccc ataggacgct gcctcgggat ccatacccgg
     5641 atacgtggtg aaacagaacc agtcgtccag agaaacataa aagcgcaacg aattgacggc
     5701 tatcttgcgc atccatttgt gcggcaggga ataaccaagc tgtatctgct tgattttaaa
     5761 ataagatccg tcaaaaacat tggcgtccga ggtccagaat ttgtcctggt cacctccacc
     5821 ggggcgggga tacctggcat ttgtgttgga agaagtccag cgttcctgat aaaataaagc
     5881 cagtttgttt ccctgcgggc ggtcaacccg gttaagcagt gagaaaatat cattcccctg
     5941 tgatcccgtt ccaaagagag tcaaatcgag tcccttccag gacatattca gggttatccc
     6001 ataagtgaaa tcaggtaatg cactccctat catgacttta tcatcgtcgt ttatgagccc
     6061 gtctccgtta ttgtccacaa agacgggatc tcctgtctta gaatcaatgt gatccagctg
     6121 gtacccgcgc atgtaccaaa cgggatagcc tacttcaaaa gccgtaagcc ccgagtaggt
     6181 atggtaactg ccacccgata tgcggctgat ggaaggatcc aggtaggtta ccttgttggt
     6241 gatggtggaa aggttccccc tgatgttgta gttgaagtca ccgattcttc cggtaaaacc
     6301 caggtcaaat tcaaacccgt ggttgtgaac gtttcctgca ttgaccgtag tgctgctcac
     6361 cccggtttca taaggaggcg tggtagccac cagaaggtct ttcgtctcct tggaaaaaaa
     6421 atctgcagtg aatgtaaggc ggtcattgaa cattctcaga tcaatcccga tatcaatctg
     6481 ttccgatgtt tcccagcgga tatccgggtt tgtcagacca gtgggtgccg atccgatatt
     6541 gtaggcaatc tcgttgtaat agggatacgt atacccggaa gctatggaag tacggtaacg
     6601 gaatcctccc agaggcccta tgttgccgtt ctgtccccag cttgccctga gtttaaggta
     6661 agtcagaacc ttgttattct ccatgaatcc ctcttttgaa atgacccaac ctgccgaaac
     6721 tgaaggaaat gttccccatc ttttttccac cggcagcacc gaggcgtcaa aagcatcccg
     6781 gcgcaatacg gcctgaagca ggtatttgtc caggtaagaa taggaaacac gtccgaaata
     6841 ggacattttg gctgatcttc caaatcctcc ccctatggtt ctgttggctg tcgggtctga
     6901 ataactgatg tcacggaaca ggggcaggtc tttggtaagt ttgtcggtcc ccgccgaagt
     6961 atagtcatta cggctttcag caaatgaaat ccctgccatg aaattgattc ggtgactgtt
     7021 tagttccaca tcataattgg caaaattttc ccactggtaa taaatgttgt tccctgaact
     7081 gcggcttaat gtcattttac tacggtaaac catatcattg gcataataca gtccgccata
     7141 cgttttgctg ttgttatatc ccagacggta accaagtttc gaagtgattg tcagtccttt
     7201 gataggagta aaatcggcgt aaactgttcc caggacattg gcgccccagg aatcggactg
     7261 cgtggcatca cgcatgatca tgggatgcac ctgttctgaa gtgacaaact gcgatacgcc
     7321 atagtaatct ccactctcat tcttaaggta atcattgtag agagccaccc agtcaggatc
     7381 attctgattg ttcttcataa actgaggcag ctgggaaggg ctgtacaggt tgggggtaag
     7441 agggtccatt gccagaacag ctgccagcaa acttccatat tcggaacttt cactcacaga
     7501 acggctgtcc catttttcca gggatgtagt cacgcctatt cgtaaccatt ttttgatttt
     7561 ataatctgca tttatctgtc ctgtcagacg gtggtacaca tcggcgtctc cggcaactat
     7621 cccattctgg tccaggtaat tgatagaagc aaaaaaattg cctctttcat ttccgccctg
     7681 gaaccccagg ctgtgacgtt gcataagcga agtttcaaaa gccacatcag cccagttggt
     7741 atcagtcacc ccatcgtatt tctgatcaaa ttcggcttgg gtaaaagtgc ctgcttcaac
     7801 catataatcc atatattgcg aagcattcat gacttccggg atcttggcca ggtcattcag
     7861 aacatattga aaatcataaa agaaactgcc ttcaccggca cgttttccgg aacgtgttgt
     7921 caccaatacg gccccattcc ctgcctgggc tccgtaaatc gcggccgaag ccgcatcctt
     7981 gagaacttct atggattcaa tattgttggg atccaggtaa ccaatatcgg aaacctgcag
     8041 tccgtcaaca agaataatgg gatcaactga gccggaattg gagcttatgc cacgaaccct
     8101 tatatccatt cctttaccgg gagcaccaga tgtggtgatg atctgcactc cggccgtttt
     8161 tccctgaagc gcttcttcta cacgggcgac agagcggttt tcaatatcag tggtattgat
     8221 ctttgcgata gctccggtca catcgctttt acgctgcact ccgtagccta tgaccactac
     8281 atcttccagt aatagagcat cttcctccag tgttatttca aaaacacggc gatctcccac
     8341 cggaatttct accgttttat atcccacaaa agacacggaa atggtagcgt tttgaggtac
     8401 agaaattaca aatttccctt cctgatctgt acttactccc cgctgcaatc caacaacggc
     8461 tacataagcc ccaggcagag ccaagcccga ctggtccaca acagtaccac tgatactgat
     8521 ctgtgcctgc tgggctgcaa cgcttgccgg aagaatcagc aataagctga aaaccagtag
     8581 gacaagcctt gtcacgggtt gataaatcat ttttttcata aaaattggtt ttggttatta
     8641 cataatggtt agttattgta ttttgttcat ttcaaatata aaactgatca actccctgtc
     8701 atgaaggtat tccatccggc actgattgtc gaataccata gtggctcccg ttcctggatc
     8761 gtagggatcc cattccggta aagaagtgac atttggattg ccgctccggg caaaattgat
     8821 ccaggcgcgg ctcatttttt cggcaaggcg gaaagcttcc tcacctcctc cggtcatgtg
     8881 ccggctcttt ttaatattgt taaaaacaaa cggtaattcc atacaatgca ttgcacgcag
     8941 tgtgccgtca agtaccgggg attcccagct gaacaggtac atatataccg gagccccctt
     9001 ctgacgtacc ttcacttctg cctgatgtaa agctccttga cgaaagcgcg tatctatatc
     9061 aaataaatct gcaggtctga atccaggata cgctgcagca actgcttcta taaaagcatc
     9121 tgttttatcc ccgtatttat cttttagaat ttcctttgcc ttttgcagat caatgtttct
     9181 cagatcaggc atataggtac tcacagagaa ttcgtgaagt gttgtaccaa taatcatagg
     9241 aatgtttttc gcagttttgg gggcctcatg atcaaaaggc tgttctttta gaaagatccc
     9301 gtctgtggtt ggagcccatc cgaagatcag aggctgaaaa ccatcagctt ccgcttcttt
     9361 tcttattttg gcaacggctt tttctcctgc acttaataat gtctggtaag gtatattttt
     9421 aatttgctct acatgtgatg gctgcaatgc cagttcacgc accacttcta taccgatctt
     9481 tctggaatat tcaggttcca tggcagatat catggagccg ctttgtacta tagctttatg
     9541 aaataatccc gaagcacgcg atgtggcgag taatgtggac actttccctc ctccgccgga
     9601 ttgcccaaac agagttacat tcttgggatc tccgccaaag tgtgcgatgt tgtccctgac
     9661 ccattccagg gccgtgatta tatccagaat acccaggttt cccgtcatgc tgtatttttc
     9721 tccgaaagcg gataaatcca gaaatccaag aatattcaac cggtgattca gggatactac
     9781 cacaatatct ccggacaaag ccagggatgc cccgtcgtat gcaggtaatt cctgtccaga
     9841 ccctgccgag tatcctcctc cgtgcagcca gaccatgaca ggacgttttt tcccgtcttc
     9901 cagtccgggt gtccagatgt tcagacgctg acagtcttct cctggaaatc cgtcatccca
     9961 attaaaagca aaagccagct cgtcgctttg ccatccggtc cttattgctt gtggacatac
    10021 cggtccgtat gccctgcagc tgcgtatacc ttcccatggt acaggacgtt gcggcaattc
    10081 aaatcgttgt gccgtagcat aaggaatccc tttaaaaata gcaagtccgt tttcaatgta
    10141 gccggctact ttacctgtaa aggtctctgc tatggcgtcc cgggaagaag ctgttacctg
    10201 ggtatcgtcc ttgttaataa acatgtgact gtctgttccg caggaacacc ctgtcacaac
    10261 aagaacaaat aagcacataa taatactgat cgtcgatttc ataagcatta ggattgcgta
    10321 attacaaaac tacagataca tgatcgttag aattgtccgg aatcattaca ttttttctga
    10381 atttgtccgc gcgaagtatg cttttgtttc ttttttgttc attttttatc acattttatc
    10441 ataattgtca gattgtgttc cgatttccgt attttcgtct tcaattcaag ttttacccag
    10501 gttatgacaa tccgacgttt ggttacaaca attttttttc tttccgtact cctctgcgga
    10561 tatacaaaca ccctatcctc aaccgagttt gccgatggat tttttcctct ttcttcaagc
    10621 ttatccaatc agaatatcag gtgttttgga gaagacaaac acggatatat atggataggt
    10681 acaggtaggg ggctgaaccg tttcaacggc tatgaatttt gccagtattt aagaagtggc
    10741 gatagtactg ctttggttga taatcaatta cagtatattt tcagggattc acaggacagg
    10801 atgtggttcc tgactgtaaa cggcttgagt ctctatggtc ctgacggaca ttttacaaat
    10861 tatcctgtag aaggcccgaa cagaaattgt attaagatcc tggaaaattc tgaagggaca
    10921 ttgtatctga gtcttgggac ttctctttct gtttttgatc cgaaaagtga agcattcagg
    10981 catgcattga atattgaatt ttccgggttc ccaaatacct ttcatatgga ccgtctggac
    11041 agattatggc ttgtaactcc tgctaaagtg ttctgttacg attcaaaaac acttgagttg
    11101 aaagcttcat gggaaacgcc gggatatatc gcatactccc atctgagaaa taacggagaa
    11161 ctctggcttg caggggaaca aatgctctcc atcctggata cccggaccgg tggttattct
    11221 tcattaccac cggggattac cggcaacagg atgttgatga actccagaat aacatatata
    11281 cagccgtaca acagaaattc aatgttgatc aatacagccg acaagggact attcctttac
    11341 ctgcctttaa cagatatatt gatccatcag agtgacaagg attttccttt tgatgttccc
    11401 gatgcagaaa ttacaacaat ttatgaagat tcacgttcta atatttttat aggaacttat
    11461 gacaaaggct tttatataag aaatcctttc cgccagtcct ttggcggaaa tacattactg
    11521 acaaaaaagt ataacgggaa aaatatagtt tcacttgata cagacagcag tgggaatttg
    11581 tggatatccg cacgccggga gggtgtatct ttatatgacc gcgttaagga ttcgattatt
    11641 gatctctcgg atatgatcga aagggtggtc cccggatttc tgaatgatcc tttgatcaac
    11701 atccatgtag ctccggataa tactgtctgg ctctatcact atacagaact gatcgctctg
    11761 aaatacaccc ggggaaatat ccgtctggta aaacggttta aatcccttcc tccagtcatt
    11821 tcagtcactt cagataaaga ggggaccttg tgggtgggga ctttcagcca ccaaatcttt
    11881 ttcctggaaa aaggatctct aacaatgaaa agccgggatt tatttcatcc cggatttgtc
    11941 tatatccccc atattttacc attatccaac ggatctcttt ttattccggg attcaaacaa
    12001 aatccaagga tcctgcatcc cggggacatg agttatgaag aactggatat aatgcaccta
    12061 ttccctgatc tgccgctgat cccgtctgcc ttgatggaag attcggcggg aattatctgg
    12121 atggggactg ttgggaacgg cctgatcaaa tatacacctg ccaccgggga aattatcccg
    12181 gtcagggatc tttcctgcaa tgacataagt gacctgttgg aagacaacgc cgggcaggtc
    12241 tggctcagca cacagtatgg attgatccgt tacgacagaa ccatagaccg gtattttagc
    12301 tatacggccc tggatggcat atcagggaac caatttaacg aaaggagtgc cgtcaggctg
    12361 cctgataaca cgctggtttt tggcgggaca catggaatca ctcatttcaa tcccctggac
    12421 attgcccaaa aaagatcggt cccgctatgt tttgaatacc tgaagatcca caacaaactg
    12481 gttatgccat ctgaagacgg acctataaaa cgctcgctga cagaaactcc ggaaatagtt
    12541 ctggaccaca ctcagaacga tttcagtata tcttttgctg cattggatta ccacaatgca
    12601 gccccgcgcg tggaatatag ctatcgttta tcgggatatg ataaagagga tatttcggcc
    12661 ggccagtacc gggaagctta ttattccaac cttcccgcag gcacgtatac atttacagtg
    12721 catataaaaa accacgacaa caccattgca gaggtatccc gatcgttgtc tttgcggatc
    12781 aagagcgctc cctggttctc ctggtgggcc tggctgcttt acgggttgtc gggtgccctg
    12841 atcctgggta gtatacttgc attgtacatg aggataaaaa gaaacaaaac aagaacccgg
    12901 caaattgaaa tggaacggga acgggagatc cgccagaatg agaccaacat gaaattcttt
    12961 tctaatattt cccatgaatt ccgaacacca ttgaccatga ttgccggccc catttctacc
    13021 ttggccaatg ataaagcaat ctccgaagag aaccggcagt tagttattat cattcagcgc
    13081 agtgtccgga gaatgctcaa gctgatcaat cagttattgg atttttataa acttgaaaac
    13141 gacacactta aactgcgggt aagaaaaaca gacatcattg agccggttaa caagttggta
    13201 gatattttca ggatcaatgc ttcagaaaaa gacataactt tacgtaccaa aggactggaa
    13261 gatacatttc ttacctggat agacgaagat aaaattgaca agatcattgg aaatctgttg
    13321 gccaatgcgg tgaaattttc tactcccgga aaaggacgta tagaggtgac attcgatgtg
    13381 atagacagta aagatgctgt aaagagcttt gatcttgacc aaagtgccgg atttgtatcc
    13441 tcctggtatg ttcaattatc ggttggcgat aacggtccgg gatttgacaa caaagacctg
    13501 gcaaaaatat ttaccaggta ttaccagatg gaacgtcaga aaaccggatt tctttcctgg
    13561 ggcacaggta taggattgta ttattcgcga cggcttgttg agactcatca cggttttatc
    13621 aaagcgcaaa acaaaccaga tggcggagct ttgttaactt ttataattcc tgttgatgaa
    13681 gccgcctatg atccttcaga actggattat tcagagacac ctgtctgtgc agatcctgct
    13741 gtaaacactt caggaccctg tcccaaaatc atgcagggag acaaagaaaa tattcaagaa
    13801 ctgcccgtga tccttctggt ggaagacgac acagatgtcg ctcattatct gaaaatgctt
    13861 ctgagaccta attaccacgt tattcatgaa tttgatgggc aaacagctct gaacattatg
    13921 gaaaccacaa ttcctgacct gataatcagc gatattgtaa tgccgggtat agacggtttt
    13981 acattttgcc gtaaaataaa agataatctt agttacagcc atatacctgt catactgctg
    14041 acagccaaaa caacgacaga agaaaaagtc agcggatttg aatccggggc agatgcctat
    14101 attacaaaac ctttcgaacc gccttacctg gaggctgtta ttaaagcaca acttaaaaac
    14161 agggaaaatt tgcaccgcct gcttgagaaa acgacaaaac ctccgcttga gattgacgat
    14221 aaggaactgt tgccccatga caaacaattt cttaagggcc tttatgagct catggaaaag
    14281 gaactctcca atccggaact caatattcag cgtatgaccg agaccctgca gatgagcagg
    14341 acaaagtttt attataaagt caaaggcctt actggtttga atccgaatgt tttctttaag
    14401 aaatacaaat tgaaccgggc agaagaactt ttgtcaaccg gtaaatacaa catatctgaa
    14461 gtggccgatc ttacaggatt cagcacatta tcccatttct ctgtcagttt taagaaacac
    14521 tttgggtcat ctcccagcga atacaaccgc aaataagcac aggcatgatg tatcctgttc
    14581 tggttcaatc ctgcagtttt ttgtacttga acggcttgat ctccgtccca agccggcgtt
    14641 tacggttttc ttcgtattca ctgtaggagc cttcaaagaa ataaacccct gaatcgcctt
    14701 caaaggccag gatatgcgtg gcaatgcggt ccaggaacca ccggtcgtgg gatacgatca
    14761 cggcacaacc cgcgaaattc tccagccctt cctccagggc tcttatcgtg ttgacatcca
    14821 catcattggt aggctcgtca agcaacagca cattggcgtt ttccttcagt gtcagggcca
    14881 ggtgcagccg gttgcgttcc ccaccgctca atatcccgca cagtttttcc tggtccgccc
    14941 ctgagaaatt gaaacgggaa acgtatgccc gcgcattcac atcgcggttc cccagacgaa
    15001 tgtattccga gtttcctgca atggtctggt aaacggtttt atcgggatct atctgtttat
    15061 gctgctggtc cacgtaggca agccgcacgg tctcccccac cctgaactcc ccggcatcgg
    15121 gtttctccac ccccattatc aggcggaaca acgtggtctt tccggcaccg ttgggtccaa
    15181 tcactcccac gatgccagcc ggaggcaacc tgaaattcag gttatcgaag aggagtttct
    15241 ccccgtaagc cttcgctaca cccgtggctt caatcacctt gtcccccagc ctcggcccgt
    15301 cgggaataaa tatttccagg cgttcttcct tctggcggct gtcctggtta agcatttttt
    15361 cataagcccc gaggcgcgcc tttgacttgg cgtggcgggc cttgggtgcc atgcgcaccc
    15421 attccagttc gcgctccagt gttttgcggc gtttgctttc ctgcttttct tccattgcca
    15481 ggcggatggt cttctgttcc agccatgaag agtaattacc tttccaggga atcccttccc
    15541 cccggtccag ttccaaaatc catccggcca cgttgtccag gaaataccgg tcgtgggtaa
    15601 cggctatgac cgttccctta tattgttgca ggtgggcttc cagccattga acgctttcag
    15661 tatccaggtg gttggtgggc tcgtccagca acagcacatc cggttcgctc aacagcaggc
    15721 ggcacaaagc cacccggcgt cgttcccctc cgctcaggta tttgacggaa ttcccggccg
    15781 gaggacactg cagggcatcc atggcccgct gcagtttgga atccaggtcc caggcgcctg
    15841 cattatcgat tttttctgtc agttcaccct ggcggatgat cagttcattc atctgatcat
    15901 cgtccatggg ttctgaaaac ttcatgttca cctc
//
LOCUS       DAOSOF010000032        15591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3793_length_15591_cov_4.394117, whole genome shotgun sequence.
ACCESSION   DAOSOF010000032 DAOSOF010000000
VERSION     DAOSOF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_15591_cov_4.394117"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1175)
                     /locus_tag="PLM68_03145"
     CDS             complement(111..1175)
                     /locus_tag="PLM68_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT54394.1"
                     /translation="MHIVTKTCSCCGNTFTTDIPRQTYCSVACRNTQNTRIKRQRKEA
                     RKARAEVKEARATLEGKAHLSITEAAKFLGVSRPTLYARIEQGELVPIRVSSRTVRIP
                     MEQLKADSHLTPQPSKGDFSVLISKAEVLEKYNISESWLMRRMKEEGFRPRIIKGKAF
                     YPKKEVDRMFKPKPVYNKEDWYNAEDLMQSEGITRKYISAYVREKKIPVQRDGRLLLI
                     SKKEWDRSRLFQGDIEKNYLTVDQAKKHYHLGQQTFYDKVKAAGLEGTRTGVFVYYKK
                     ADLDRLFKDKTPKIPAEIRKNYMRSGDALKYYHLGQKRFSEETQAAGVTKVRTEGNYV
                     WYKKDELDKLFKKISDNGNN"
     gene            complement(1464..2978)
                     /gene="mnmE"
                     /locus_tag="PLM68_03150"
     CDS             complement(1464..2978)
                     /gene="mnmE"
                     /locus_tag="PLM68_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOT54395.1"
                     /translation="MNTKEPVTGSTGTSGNSGTNSEPATSIYAPVTTICAPATPHGVG
                     ALALIRMSGPQALEIAGKVFHSSACPDLAQSEGYRTYFGRVNDPAGELLDEVLLSVFR
                     APHSYTGEDSVEISCHGSLYIQDALLRTLVENGAVMAGPGEFSQRAFMNGKMDLAQAE
                     AVADLIASENRTSHKLAMDQMRGVFSQELSVLRSRLLELASLMELELDFSDEDVEFAD
                     KQELRSLVDSLLVRIGKLCDSFSMGNAMKKGVPVAIAGLVNSGKSTLLNALLGEEKAL
                     VTPVAGTTRDTIEDVLVVDGIPFRFIDTAGLRHIGEVDEMSQEHVEKLGVKRSLETIR
                     SAAVLMLVLDASRPETYTESLQSVAVFKNEHILFIINKIDLVCNIDVTLAGFMNTLCN
                     TYVTLCKSNAVIIPVSASKGVGIEDIKNTLVAKIRPDLDRNAIILTNLRHYQELRAAG
                     EALQRVREGLDARLSTDLLTPDLRQALHHIGSITGEITPDDILGEIFGRFCIGK"
     gene            complement(2975..4549)
                     /gene="rlmD"
                     /locus_tag="PLM68_03155"
     CDS             complement(2975..4549)
                     /gene="rlmD"
                     /locus_tag="PLM68_03155"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT54396.1"
                     /translation="MGKRDREILENICIESVAAEGNALAHVNGKALFVPMAVPGDIVD
                     VQVTHKRRSYMEGNILKLVTPSPMRLEPFCSHFGDCGGCRWQPIPYDKQLEFKQQQVI
                     DQLTRIGRLTLPPVHPILGSEKTVQYRNKLEFTFSNNRWVPATASPAPTPTPTTTATA
                     TATVTATTSAYTVPGPVFTTEQEVDHLPLPVPLPQDQFPPLPAPALGFHVRGRFDKVL
                     DIETCHLQAEPSNAIRLAIRDYAIEKGLSFYDLRKQTGFLRTLMIRIILSGEVMVVVV
                     FAHDNPAAGEDHEARKALLDHVMQQFPQITSLHYVINTKLNDSLSDQEAIHYAGKPYI
                     VETLDDLQFRIGPKSFYQTNSLQALQLYRKVLEYADLKGDETVYDLYTGTGTIALFLA
                     RHCRSVTGIEYVQEAIEDARENASANHIENAVFFAGDIKDVLTEDFIASKGYPDVVVL
                     DPPRAGVHEDVLKVLMETAPQKIIYVSCNPATQARDLALLQQQYRITHVQPADMFPHT
                     HHVENIVVAEKVLHIS"
     gene            4668..5906
                     /locus_tag="PLM68_03160"
     CDS             4668..5906
                     /locus_tag="PLM68_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT54397.1"
                     /translation="MSILFVSGAVFSLQAQENTLYISGKVVDSETKRPMVFASVSIQD
                     VGISIVTNGEGFFTLKFSRVYEDKNVNISYLGYHTSVIPVKQFLGQEKTPIIALIQAA
                     LPIPMTTVRPQDPLELVREAFRRIKDNFPQDRMQMTGFYREMIRKGNTYVTLSEAVVD
                     VYKPPYTPTFAVDQVGIYKGRGSVDWARIDTVFVKFQGGIRSALELDVARNLFMGVNL
                     TEVDKFYDFKIENPVQMNDRINYVISFDQKPGQEDILFRGKMFIDVETMAISRVEFNI
                     NVENRPQATAIFIRRKPAGLRAQVEYAAYLVQYQQIPGGQWVFNYSRTDLKFNAKWDS
                     KLFRQNYTITSEMAMTEYSRESYKIPQESRVKTTDITLYRVADFEDAEFWEDYNIIEP
                     ESSIENVISRIIRQLKRRQR"
     gene            6010..7296
                     /locus_tag="PLM68_03165"
     CDS             6010..7296
                     /locus_tag="PLM68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT54398.1"
                     /translation="MLNSNIPLAERMRPSSFSQFVGQEHLVAPDAVLRKMIESRNLAS
                     FILWGPPGVGKTTLARIIAQDLERTFYSLSAVSSGVKDVRQIIDRSRSNKMFQKNSPI
                     LFIDEIHRFSKSQQDALLGAVEDGTVVLIGATTENPSFEVISPLLSRCQVYVLKPLGV
                     KDLETLFERALKEDEYLKTLNIQVEETEAIFRLSGGDARKLLNIIEIVVSSYDQGETI
                     VFNDEEVQKRLQKNIVLYDKNGEQHYDIISAFIKSIRGSDPNGAVYWLARMISGGEDP
                     LFIARRMVILASEDIGLANPNAFLMANACFDAVHKIGMPEGRIPLSQTAIYLATSAKS
                     NSAYRAIEEAFESVMRHGDLSVPLHLRNAPTELMKELGYAKDYKYAHDFEQNFVIQEF
                     LPAEISGTSFYHPQDNAREKEISRFLERWGNKYSKV"
     gene            7322..8137
                     /gene="kdsA"
                     /locus_tag="PLM68_03170"
     CDS             7322..8137
                     /gene="kdsA"
                     /locus_tag="PLM68_03170"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOT54399.1"
                     /translation="MDLDNFFLIAGPCVVESEKLCMEVAEKLCFLTAKYNLRLVFKAS
                     YRKENRTRVDSFTGIGDHRGLEILQTVKNYFNLIVTTDVHTPDEALMATEYGIDIIQI
                     PAFLCRQTSLLKAAAQTGKAVNVKKGQFLSPTQMQFVVEKLLQFGATMERIILTERGT
                     QFGYSDLIFDVRSIPIMKRLNVPVVLDVTHSLQMPNQVNGVSCGDPSLISTLAAAGVA
                     AGVDGLFVETHPRPMDALSDGNIMLPLDQMESFIRRIIAFRKTYLNIEKESNT"
     gene            8143..10050
                     /locus_tag="PLM68_03175"
     CDS             8143..10050
                     /locus_tag="PLM68_03175"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT54400.1"
                     /translation="MTHIQDLNTRYENLPVFIYDDQVEASTYLVDTILNLLSLKESQG
                     KKLVLGLSARTVMIPLYELFARAYEHGRSFKNVVIFGINEYYPLSENEIQSHYRFLRE
                     YLFDVLDIPKNQIHCLKGDIPRSQVSRHCADFEKKIASYGGIDVLITSGMGSNEPGSH
                     YSSRTRLITLNTNTRIAAGSEFFGVDFVPNYSLTMGIQTILESKTIYYLAWGEGESKA
                     IKDLVEGKVDESHPSSFLQNHAQTEVIIDRAAAADLTRIATPWLTGPCVWTEELTRKA
                     VFWLCRLLDKPILKLTARDYNDNGMSDLITERGPFSKINIDTFNSLQHTITGWPGGKP
                     NADDTTRPERALPYPKRVIVFSPHPDDDVISMGGTVARLSQQGHILHLAYQTSGNIAV
                     FDHEVTRFLDFLQEISPVYGLDQEKTRTIFEQASRDLEKKKPGEADTHAIRKVKELIR
                     RGEAKSAALYLGIAHENIHFLEMPFYETGGIKKKPLSDKDIDIVCNLLEKIQPHQIFA
                     AGDLSDPHGTHRVCYTSIIKALEKLRDRPWTSDCRLWLYRGAWQEWDVADVDMAVPLS
                     PEEVIIKRNAIFRHQSQKDPAMFPGNDPREFWKRAEERNHNTAVLYDKLGMAEYQAIE
                     MFRLYKEGMKI"
     gene            complement(10047..12278)
                     /locus_tag="PLM68_03180"
     CDS             complement(10047..12278)
                     /locus_tag="PLM68_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT54401.1"
                     /translation="MRKGVFLMILFLSGCAGTRYVPQGEYLLRKNTLEVVPEKIVSPS
                     SLQSYIRQKPNTSIIFGWKAFLNIYSLSPDKDNGWSRFLRRMGEPPVVFDPSLITYSK
                     LNLDNYLTSLGFYFNTITDTVTYRNRKASVHYTIMPGKTYTIDTIRLGKADTNLAALY
                     LQHQDRAVIGPGHILSSQTLEREAARINSVMRNNGYYSFNRSLVSFLADTLRQNGTAT
                     LEVQLPGNGEHKPYKIRKINVYPGFDPIQAVMDTAYYKSVESRDYEGMTFHYNGKTTI
                     RPRIISNMSFIRPGELYDESKVKTTYDRLSSIRLMNSVTLLFDEVSSSRQQDTAEVDC
                     TIRMIPGNSQGYKVNLEASSNSNGLIGISPALSYYHKNIFRGGEWLTLALMGNFQFKL
                     KNPIRATELGASLSLTFPKMLFPVSNRIFSGRIPRTEVTASYSYQSRPEYTRNIASGT
                     FGYTWSMGDKFHYTVNPVKMKLVKLYNMSPGFLESLNDPFLIDTYRDHLDLGLSSIFY
                     YTSDNSPVHNTTWHYVRVGLEAAGNLLSAFNSLMKADSTGSHMIAGIAYSQYIKIDIN
                     SGYTLIRNRHQLAGRFYAGVGVPYGNAASLPLEQMFYAGGANSLRAWQARTIGPGSMP
                     LDSTFSIPNQAGDIRLELNLEYRFPLFWKLEGALFADAGNIWTLPRGNAAPAGVFRFS
                     DFYRSIAMDAGLGIRVNLDFVILRLDLGMIMRDPVKQAWVPPSRWLKRENYSFQFGVG
                     YPF"
     gene            12399..13787
                     /locus_tag="PLM68_03185"
     CDS             12399..13787
                     /locus_tag="PLM68_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOT54402.1"
                     /translation="MKNKYIDLIQQSFDFPQEEFRIEDGELLFHDIPVMDLIDQYGTP
                     LKFYYLPSISKQIQKAKRLFNVAMAKVDYEADYHYCYCTKSSHFSFVLEEALKNDIHI
                     ETSSAFDINLIEALHEQKIVDKDLYVICNGYKKQQYIENIAKLVNSGWENTISILDNM
                     PEIEALDNAVQGECKIGIRIAAEEEPKFEFYTSRLGIRYSDVVPYYHKYIKRNNKFKL
                     KMLHFFINTGMQDNAYYWNELAKCVNVYCDLKNFCPELDSLNLGGGFPVRNSLSFDYD
                     YEYMTEEIIAQIKSICTSRNVKEPDIFTEFGSFTVGESGANIYQVLGEKQQNDRERWY
                     MIDSSFMTTLPDTWGIKRRFILLPINKWNEPYQRVFLGGLTCDSQDYYNAEAHANAVF
                     LPKNENKDPLYIGFFNTGAYQESISGFGGVQHCLMPAPKMVIIGEENGTYYTKLFSKE
                     QTYKSMLKLLGY"
     gene            13792..14544
                     /locus_tag="PLM68_03190"
     CDS             13792..14544
                     /locus_tag="PLM68_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT54403.1"
                     /translation="MLSKAKIQYIKALEQKKFRQERQEFILEGRKMVLELLKAPGPFR
                     IKELYLQEDFPEPVQSSVAVYISAKEMERISLQSTPSGALAVVEIPKVPLAPPRKDNL
                     YLALDGIRDPGNFGTILRLADWFDLAAVYASPDCVELYNPKTVQSTMGAILRVPVHYT
                     PLAPLLKNCTLPVTGSVLQGGSSINTTFLPCDGIIVLGNESNGISPELLDHIPLKLYI
                     PPYREGSESLNVATAAAIFCAVFRRNLPSLPR"
     gene            complement(14483..>15591)
                     /locus_tag="PLM68_03195"
     CDS             complement(14483..>15591)
                     /locus_tag="PLM68_03195"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT54404.1"
                     /translation="LDSITAILFARLRYEHKAFRFAVFKTIKICCELGFNLLFFLWAP
                     RYLSANPEAFLGRFITAVPDYNYVLFSIFLSCIVALLLFLPDLLKRSSFILDRKLWPK
                     LMMYSLPLMIAGLPGILNDFSDRLLFRFLIPGDVSWRAQMGIYQAGIKLAVLINLFIQ
                     MFRYAAEPFFFGQSAEKEAPRTYARVLDIFTAICMLVYLGILLYMDLFELILGENYRE
                     GIVVVPVMALAYVLLGIYFNISIWFKLSGKTGNAVLITSAGLAVTLLVNLLFMPRYGY
                     MAAAWGHLASYLSMTVLCALMGRKHYPIPYNWLKIGFYLVAGVGLYLFSLMFAHLSMI
                     PKLAVNTGIILVYLAIWAGMEGFYGKLRKILQQR"
BASE COUNT         4062 a         3786 c         3856 g         3887 t
ORIGIN      
        1 ggtattcgtg ccggacgtac ttgcgtgagt gcgggtctct caggggcggg tagaagtcga
       61 agtagagcgt aatcttgccg cttggcagca gccgctggcg tagtgttact ttagttgttc
      121 ccattatcgc ttatcttttt gaataacttg tcgagttcat ctttcttgta ccagacgtag
      181 ttgccttccg tccggacttt ggtcacgcca gcggcctgag tttcttcgct gaagcgtttc
      241 tggccgaggt ggtagtattt gagtgcgtcg ccgctgcgca tgtagttctt gcgaatctca
      301 gcggggattt tcggggtttt gtctttgaag aggcggtcga ggtcggcttt cttgtagtag
      361 acgaagacac cggtgcgggt gccctcaagg cctgccgcct ttactttgtc gtagaaagtt
      421 tgctggccga ggtgatagtg tttcttggcc tggtctacgg tgaggtagtt cttctctatg
      481 tcgccctgga agaggcggct gcggtcccat tctttcttgg aaatcaggag taggcgtccg
      541 tcccgctgga caggaatctt cttttcgcgg acgtaggcgg agatgtattt gcgcgtgatt
      601 ccttcgctct gcatcagatc ctcagcgttg taccagtctt ccttattata tacgggcttg
      661 ggcttgaaca tccggtccac ttcctttttg gggtagaagg ctttgccttt gatgatgcgc
      721 gggcggaagc cttcttcttt cattcgtcgc atcagccagg attcggagat gttgtacttc
      781 tccaagacct cggcctttga tatgagcacg gagaagtcgc ctttgctggg ctggggcgtc
      841 aggtggctgt ccgctttgag ctgctccatt ggaatgcgga cggtgcggga agagacgcgg
      901 atagggacga gttctccttg ctcgatgcgg gcgtacaggg tagggcggga taccccgagg
      961 aatttggcgg cttcggtgat tgagaggtgg gcttttcctt cgagcgtcgc tcgcgcttcc
     1021 ttcacctcgg ctctggcttt gcgtgcctct ttgcgctggc gcttgatgcg ggtgttctgc
     1081 gtattgcgac aggcgacgct gcagtacgtc tgcctcggga tgtccgtggt aaacgtgttg
     1141 ccgcagcagg agcatgtctt tgtcacaatg tgcatacttt ttacatcatt ttggcggaga
     1201 tgaggcgcaa gaccttcaac gtgccgtttg acccttctaa ttgggtacaa gtttgtaaat
     1261 ctacgtaaac ctatgtaaag ggatggtatt cggccggtgc cctccatagt cttgaaccac
     1321 gcgtggttca aatgtggttc aaatatagca aaaaaatccc gaattagccc aaactaatcc
     1381 ggaaaagttt tcaacatttt atcgttgatt atgtgagagt taggaggttt tatacggggc
     1441 gcgtagaggg tggttcggtg tatttatttt cctatacaga aacgcccaaa gatctcaccc
     1501 aagatgtcat ccggggtaat ctcaccggtt atgctgccga tatggtgcag ggcctggcgc
     1561 aggtccgggg tgagcagatc ggtgctcagc cgggcatcca ggccttcgcg aacgcgttgc
     1621 aaggcttctc cggcagcacg aagttcctgg taatggcgca ggttggtcag tatgatggcg
     1681 ttccggtcca gatccggccg gattttggct actaaggtgt tcttaatgtc ctcaatacca
     1741 acacctttag acgcagatac cggtatgata acagcgtttg atttgcagag tgtaacataa
     1801 gtgttacata gtgtgttcat aaatccagcc aatgtaacat ctatgttaca tactaggtca
     1861 attttattaa taatgaacaa gatgtgttca tttttaaaca cagcaaccga ttgcaggctt
     1921 tcagtgtagg tttccggccg ggaagcgtcc agaacaagca tcaaaacggc tgcagaacgg
     1981 atggtttcca gggaacgttt cacgcccagc ttttcaacat gttcctggga catttcgtcc
     2041 acctcgccaa tatgccgaag cccggccgta tcgatgaacc ggaaggggat cccatccacc
     2101 accagcacat cttcaatggt atcgcgcgtg gtaccagcca ccggggttac caacgccttt
     2161 tcctcgccca gcaaggcgtt cagcagggtg gatttcccgg agttcaccaa tccggcaatg
     2221 gcaaccggga cgcctttttt catggcattt cccatactga aagaatcgca aagtttgccg
     2281 atgcgtacca gcagcgaatc caccagggag cgtagctcct gtttgtctgc aaattccacg
     2341 tcttcatcgg aaaagtccag ttccagttcc atgagcgagg ccagctccag cagacgggac
     2401 cgcagcacgg aaagttcctg ggagaaaacg ccccgcatct ggtccatggc cagtttgtgg
     2461 gaagtgcggt tttccgaggc aatcaggtcg gccacagcct cggcctgcgc cagatccatc
     2521 ttgccgttca tgaaagcacg ctgggaaaat tcccctggac cggccattac tgcaccgttt
     2581 tcaaccaggg tacgcaacag ggcgtcctgg atgtaaagtg acccgtggca cgagatttcc
     2641 acgctgtcct cgcccgtgta ggaatggggt gccctgaaaa cgctgagcaa cacctcatcc
     2701 agcagttccc cggccgggtc gttcacacgc ccgaaatacg tgcggtaacc ctcgctttgt
     2761 gccagatccg ggcaggcaga cgaatgaaag actttcccgg caatctccag cgcctgtggc
     2821 ccgctcattc gtatcagcgc caaggcgccc acgccgtgcg gggtagccgg agcgcaaatg
     2881 gtcgtaaccg gtgcgtaaat ggacgtggcc ggttcgctat tggtgccgga attgccagat
     2941 gtcccggtgc tgccggttac gggttctttt gtgttcatga tatatgcagg actttctcgg
     3001 caacaacaat attttcaacg tggtgcgtat gggggaacat gtctgccggc tgtacgtgcg
     3061 ttatccggta ctgctgctgc aacagggcca ggtcgcgggc ctgggtggcc gggttgcagc
     3121 tcacatatat gatcttttgc ggagccgttt ccatcaaaac cttcagcaca tcctcatgca
     3181 ccccggcccg cgggggatcc agcaccacca catcgggata accctttgaa gcaatgaaat
     3241 cctccgtcag cacatccttg atgtctccgg caaaaaagac ggcattctca atatgatttg
     3301 ccgaggcatt ctcccgagca tcttctatag cctcctgcac gtactcgatg cctgtcacgc
     3361 tgcggcagtg ccttgccaga aacagggcaa tggttcccgt ccccgtatac aggtcgtaca
     3421 ccgtttcatc gccctttagg tcagcatatt cgagtacctt ccggtacagc tgcagcgcct
     3481 ggaggctgtt ggtctggtag aaggatttgg ggccgattcg gaattgcagg tcatccaggg
     3541 tttccacaat atacggtttt cccgcataat ggatggcttc ctggtctgag aggctgtcgt
     3601 taagcttggt attgatcaca tagtgcagcg atgttatctg cgggaattgc tgcatgacat
     3661 gatccagcag tgccttgcgg gcctcatggt cttctcctgc ggccgggtta tcgtgcgcaa
     3721 aaaccactac caccatgact tctccgctca gaatgatgcg gatcatcagg gtacgcagga
     3781 atccggtttg tttccggaga tcatagaaag agagaccttt ttctatagcg taatccctga
     3841 tggccaggcg tatggcattc gaaggctctg cttgaaggtg gcatgtttca atgtccagga
     3901 ccttgtcaaa ccggcctcgg acgtgaaaac ccagggcagg agcaggtagg ggcgggaatt
     3961 ggtcctgggg aagaggcaca ggcaacggca gatggtcaac ttcctgttcc gttgtgaata
     4021 caggtcccgg aaccgtataa gcggaagtag tagcagtaac agtagcagta gcagtagcag
     4081 tagtagtagg agtaggagta ggagcaggag aagcagtagc tggcacccac cggttgttgg
     4141 agaaggtgaa ttccagcttg ttacggtact gtaccgtttt ttcagaaccc aagatggggt
     4201 gcacaggagg taaggtcagt ctaccaatcc tggtcaattg gtcaatcact tgttgttgtt
     4261 tgaattccag ctgcttatcg tagggtatgg gttgccagcg gcagcctccg caatccccga
     4321 aatggctgca gaatggctcc aggcgcatgg gtgaaggcgt taccagtttc agtatgttgc
     4381 cttccatata actacgtctc ttgtgagtta cctgtacatc aacaatgtca cctggcacgg
     4441 ccatgggcac aaagagtgcc ttgccattga catgggccag ggcattgccc tcggctgcta
     4501 ctgattctat acagatattt tccaggattt ccctgtctct ttttcccata aggttcattt
     4561 acaattggca aaaatgcgaa aaaaaaagcg tattttagga gaattaataa aacagccaga
     4621 ccatgaaaac gtacattatc ttaaaaaatg cattcaaaat gctgataatg agcattttgt
     4681 ttgtatctgg agctgtgttt tcattacagg cccaggagaa tacgttatat atctccggaa
     4741 aggtagtgga ttcagaaaca aaacgtccga tggtttttgc atcggttagt atacaggatg
     4801 ttggcataag cattgtcacg aacggagaag gtttcttcac cctgaaattt tcccgtgtat
     4861 atgaagacaa aaatgtaaat atcagttatt taggttatca tacaagtgtt atacccgtca
     4921 aacagttcct gggccaggaa aaaacaccaa tcatagcctt gatacaggct gcgttgccca
     4981 tacccatgac cacggtaagg ccgcaggatc ccctggaact ggtccgggaa gctttccgca
     5041 ggataaagga caattttccg caggatagaa tgcagatgac aggtttttac cgtgaaatga
     5101 tccgcaaagg caatacgtat gtcacactgt cggaagccgt cgtggacgtg tataaaccac
     5161 cgtatacacc cacatttgcc gtcgaccagg tgggcatcta caagggtcgt ggaagtgttg
     5221 actgggcaag gattgacacg gttttcgtca aattccaggg cggcatccgc tcggctctgg
     5281 aactggatgt ggccaggaac ctcttcatgg gtgtgaacct gacagaggtg gataagtttt
     5341 acgatttcaa gattgaaaat cccgttcaga tgaacgaccg catcaattat gtgatctcgt
     5401 ttgaccagaa acccggccag gaagacatac tgttccgggg caagatgttc attgatgtgg
     5461 aaaccatggc gatttcccgc gttgaattca atatcaatgt cgaaaaccgg ccacaggcga
     5521 cggccatttt cattcgtagg aaacctgcag gcttgagggc gcaggtggaa tacgccgcct
     5581 atttggtaca ataccagcag ataccgggag gacaatgggt attcaattat tccaggacgg
     5641 atttgaagtt caatgccaag tgggacagca aactgttcag gcagaattat accatcacct
     5701 cggaaatggc catgaccgag tacagccggg aatcttacaa gatcccgcag gagagccggg
     5761 tcaagacgac agacatcaca ttgtacaggg ttgcagattt tgaggatgct gaattctggg
     5821 aagattacaa catcatagaa ccagaatctt ccatcgagaa tgtgatatcc cgtataatac
     5881 ggcaactaaa gcgccgccag cgctaaaagc ctataagtac tattatgcga agaataccgc
     5941 cgataattgt aaaatgcaca caatttatat tatgttaaat aggtttttta caataaaaaa
     6001 aagaactaca tgctgaacag caacattccc ctggctgaac gcatgcgccc ctcctctttc
     6061 agccagtttg tggggcagga acacttggtg gctccggatg ccgtattgcg aaaaatgatc
     6121 gaaagccgga accttgcctc atttatcctc tggggacctc ctggtgtcgg taaaacgacg
     6181 ctggcacgca tcatagcaca agacctggaa cgtaccttct acagtctgtc agcagtaagt
     6241 tctggagtca aggacgttcg ccagatcatc gaccgttcac gttcaaataa gatgttccag
     6301 aaaaacagtc ccatcctgtt catcgacgaa atacatcgtt tcagcaaatc acagcaggat
     6361 gccctcctgg gtgccgtgga agacggaact gttgtcctta ttggtgccac taccgaaaac
     6421 ccttcttttg aagtgatctc tcccctgctt tcacgctgcc aggtttacgt tcttaaacca
     6481 ctgggcgtaa aagatttaga aactttattt gaacgggctc tgaaagaaga tgaatacctg
     6541 aaaacgttga acatccaggt tgaagaaacc gaagccatct tcaggctttc cggcggagat
     6601 gcacgtaagt tgttgaatat tattgaaata gttgtttcca gctacgatca gggtgagacc
     6661 atcgtcttca atgatgaaga ggtgcagaag cggttgcaga aaaatatcgt gctttacgac
     6721 aagaacggag agcagcacta cgatatcatc tcggctttta tcaaaagtat ccggggaagc
     6781 gatccgaacg gggcggttta ctggctggcc aggatgattt cgggcggaga agatcccctt
     6841 ttcattgcgc gcagaatggt catccttgct tctgaagata tcggtctggc gaatcccaat
     6901 gcctttctca tggccaatgc ctgcttcgac gctgttcaca aaatcggaat gccggaaggc
     6961 cgcattcccc tctcccagac tgccatatat cttgccacga gtgccaaaag caactcggcc
     7021 tatagagcca ttgaggaagc ttttgaaagc gtcatgagac acggagattt gagtgtgccg
     7081 ctgcatttac gcaatgcccc caccgaattg atgaaagaac ttgggtatgc caaagattat
     7141 aaatatgcac acgattttga acagaatttt gtgatacagg aattccttcc cgcagagatc
     7201 agtggtacct ctttctacca tccacaggac aatgcaaggg aaaaagagat aagcaggttc
     7261 ctggagcgct ggggaaacaa atattcaaaa gtgtaataaa aaatatacta tctttgatgt
     7321 tatggatctg gataattttt ttctaatagc cggcccttgt gtcgtggaat ctgaaaaact
     7381 atgcatggaa gtggccgaaa agctttgctt cctgactgca aagtacaact tacgcctggt
     7441 tttcaaggct tcctatcgta aagaaaacag gacacgggta gattcgttta ccgggattgg
     7501 agaccaccgg ggactggaaa tactgcaaac agtaaagaat tatttcaacc ttattgtcac
     7561 cacagatgtg catacacccg atgaagccct tatggctaca gagtacggga ttgacataat
     7621 ccagattccc gcctttctct gcaggcagac atcattgctg aaagctgcag ctcagactgg
     7681 aaaggcagtg aatgtgaaaa aaggccagtt tctgtctccc acccagatgc agtttgttgt
     7741 agaaaaactg ctccaatttg gagccactat ggagcggatc atccttacag aaagaggcac
     7801 ccagtttggt tattcagacc tgatctttga tgtgcgcagc atccccatca tgaaaagact
     7861 gaatgttccc gtagtccttg acgtaaccca ttcccttcag atgccaaacc aggtcaatgg
     7921 cgtaagctgc ggagatccat cacttatatc aactttggcg gcagctggtg tggcagccgg
     7981 tgtggacggc ctgtttgtcg aaacccatcc caggccgatg gatgcccttt cggacgggaa
     8041 catcatgctg ccgctggatc agatggaatc attcatacgc aggatcatag cctttcgcaa
     8101 aacgtacctc aatattgaaa aagaatccaa tacttgaaat aaatgacaca catccaggac
     8161 ctcaacacac gttatgagaa tctgccggtt ttcatatatg atgaccaggt agaagcttca
     8221 acataccttg ttgacaccat actgaacctg ttaagcctta aagaatccca gggaaagaaa
     8281 ctcgtgctgg gcctgtcagc gcgtacggtc atgattccgt tatatgagct ttttgcgcgc
     8341 gcttatgagc atggcaggtc tttcaagaat gtagtcattt tcggtataaa cgaatactat
     8401 cctttgtctg aaaacgagat acagtctcat taccgtttcc tgagagaata ccttttcgat
     8461 gtgctggata ttcctaagaa ccaaatacat tgtcttaaag gggatattcc ccgctcccag
     8521 gtatcacgtc attgtgccga ttttgaaaag aagatcgcct catacggagg tattgatgta
     8581 ttgataactt cagggatggg ttccaatgaa ccaggttccc actacagctc ccgaacgcgt
     8641 ttgatcaccc tcaatacaaa tacccgcata gccgctggca gtgaattctt tggggtggat
     8701 ttcgttccca attattcgct gactatggga attcaaacca ttctggaaag taaaaccatt
     8761 tattacctgg cctggggaga aggagaatcc aaagcaatca aggacctggt agagggaaag
     8821 gtagacgaat cccatccttc cagtttcctg caaaatcacg cacagacaga ggtcattatt
     8881 gacagggctg cggcggccga ccttacccgc attgcaacac cctggcttac agggccctgt
     8941 gtatggaccg aagaacttac ccgcaaagct gttttctggc tctgccgcct gttggacaaa
     9001 cccatcctga aactcaccgc ccgtgactac aacgacaacg gtatgagcga tctgatcacc
     9061 gagcgcgggc ctttcagcaa aatcaacatt gacactttca acagcctcca gcacaccata
     9121 accggctggc cgggaggaaa acccaatgca gacgatacca cccgtcccga aagggccctg
     9181 ccctacccta aacgtgtgat tgtcttcagc ccccaccccg atgacgatgt gatttcaatg
     9241 ggtggtaccg tggcacgtct ttctcagcag ggtcatatcc tgcacctggc ttatcagact
     9301 tcaggaaata ttgccgtatt tgaccatgaa gtgacacggt tcctggattt cctgcaggaa
     9361 atatctcccg tttacggact ggatcaggaa aagacacgta cgatctttga acaggcttcc
     9421 agggatttgg agaaaaagaa accgggagaa gccgatacgc atgccatccg taaagtgaaa
     9481 gagctcatcc gtcggggaga ggcaaaatcg gctgcactgt acctgggcat cgctcatgag
     9541 aacattcatt tcctggaaat gcccttttat gaaaccggtg gtatcaagaa aaaaccttta
     9601 tccgataagg acattgacat tgtctgcaac ctgctggaaa agatccagcc acaccagata
     9661 ttcgcagctg gcgatttaag cgatccacac ggcacacacc gcgtttgcta tacctccatt
     9721 atcaaagcat tggaaaagct ccgtgacagg ccgtggacaa gcgattgccg cttgtggctc
     9781 taccgcggtg cctggcagga atgggatgtt gccgatgttg atatggcggt tcccctgagc
     9841 cccgaggaag tcatcatcaa gcgcaatgcc atcttccgcc accagagcca gaaagatccc
     9901 gccatgtttc ccggtaacga cccgcgggaa ttctggaaaa gggctgaaga gcgcaaccat
     9961 aatactgctg tgctctatga caaactgggc atggccgaat accaggccat agaaatgttc
    10021 aggctgtaca aagaagggat gaaaatctaa aaagggtatc ccacgccaaa ctggaatgag
    10081 taattctccc gcttcagcca ccgggagggc ggaacccatg cctgttttac cggatctctc
    10141 attatcatgc caaggtccag ccgcaggatg acaaaatcca ggttgaccct gatgcccagt
    10201 ccggcgtcca tcgcaatgct cctgtaaaag tcgctgaaac ggaaaacacc cgccggagct
    10261 gcgtttcccc ggggcagggt ccagatgttc cctgcatcgg caaataaggc tccttccagt
    10321 ttccagaaaa gaggaaaacg gtattcaagg ttcaattcaa gccttatatc gcccgcctgg
    10381 ttcggaatgc tgaaagtcga atccaagggc attgagcctg gtcctatggt tcttgcctgc
    10441 catgccctga ggctgttggc tcctcctgca taaaacattt gctccagggg cagtgaggct
    10501 gcattcccgt aaggtacacc taccccggca taaaagcgac ctgccagctg atggcggttc
    10561 cttatcaggg tatagcctga attgatgtct atttttatat actgtgaata cgcaatcccg
    10621 gctatcatat ggcttcctgt gctatctgcc ttcatcagtg aattgaaagc gctaagcagg
    10681 tttcccgcag cctcaaggcc cacccttacg tagtgccatg ttgtattgtg cacaggtgaa
    10741 ttgtcgctgg tgtaatagaa aattgatgaa agacccaggt ccaggtggtc cctgtatgtg
    10801 tcaatcagaa acgggtcatt gagtgattcc aggaaacccg gactcatgtt gtacagtttg
    10861 accagtttca ttttaacggg attcaccgta taatggaact tgtctcccat gctccatgta
    10921 tacccgaaag tgcctgaagc aatgttcctt gtatattctg ggcgggactg gtagctgtaa
    10981 cttgccgtga cttccgtgcg tggtatcctt cctgaaaaga tcctgttgct taccggaaaa
    11041 agcatttttg gaaaggtcag cgaaagtgag gcacccaatt cagtagcccg gatgggattc
    11101 ttcaatttga actgaaaatt tcccatcagt gccagggtca gccactcccc accccggaat
    11161 atgtttttat ggtaatatga cagtgcgggc gagatcccta taagaccgtt cgaattgctg
    11221 gatgcttcca gattcacttt gtaaccctgg gaattgccag gaatcattcg tatggtacag
    11281 tcaacttctg ccgtatcctg ctgtctcgaa gaagatacct cgtcaaaaag cagcgtaaca
    11341 ctgttcatca gcctgatgct tgacagacgg tcatatgttg ttttcacttt ggattcgtca
    11401 tacaattctc ccggtcggat aaaactcatg ttactgatga tacgcggcct tatcgtggtc
    11461 ttcccgttgt aatggaatgt catgccttca tagtcccggg actccacgga tttataatat
    11521 gccgtgtcca ttaccgcctg aataggatca aaacccgggt aaacgttgat tttccttatc
    11581 ttataaggtt tatgctcccc attacctggc aattgaactt ccagcgtggc tgtgccattc
    11641 tgcctgagtg tatcggccag gaaagaaacc aggctgcggt tgaaactgta atacccgtta
    11701 tttctcatta ctgaattgat gcgggcggct tccctttcca gtgtctggga agagagtatg
    11761 tggcccggcc ctatcacagc acggtcctgg tgctgcaaat ataaagcagc caggttggta
    11821 tctgccttgc ccagccggat ggtgtctatg gtgtatgttt ttcctggcat gatggtgtaa
    11881 tggaccgagg ctttcctatt ccggtaagta accgtgtcgg tgatggtgtt gaaataaaaa
    11941 cccagagagg tcaggtaatt gtccaggttg agcttgctgt aggttatcag ggaaggatca
    12001 aagacaacgg gaggctctcc catacggcgg agaaaacggc tccagccgtt gtctttgtca
    12061 ggactcaggc tgtagatatt caagaatgct ttccagccga aaataatgct ggtatttggt
    12121 ttttggcgaa tgtatgattg cagggatgaa ggggatacga tcttttcagg taccacttct
    12181 aatgtattct tgcgcaacag gtattctccc tgtggaacgt atcgcgtgcc tgcacacccg
    12241 gacagaaaca ggatcatcag aaaaacacct ttacgcatat tgcaaaagta aaaaaaaagg
    12301 tcaaatacga tatcacaaat aattattaac atatatttgc aatctgattg acttacattg
    12361 attatttttt gataaagtaa ctcacttaca ttacttcaat gaaaaacaag tacattgacc
    12421 tgatccaaca gagtttcgat tttccgcaag aggaattcag gattgaagac ggtgagcttt
    12481 tatttcacga tattcctgtc atggacctta tcgatcaata cggaacgccc ttgaaattct
    12541 attatttacc ttccatcagc aaacagatcc aaaaagcaaa aaggttgttc aatgttgcca
    12601 tggccaaggt tgactatgaa gcggactacc attactgcta ttgtaccaaa tcatcccatt
    12661 tttcatttgt actggaagaa gctctcaaga acgacatcca cattgagact tcctctgcct
    12721 ttgacattaa cctcatagaa gcgctccatg aacaaaagat cgtggacaag gacctgtatg
    12781 tcatctgcaa tggttacaag aagcagcaat acatagagaa tattgccaaa ctcgtgaatt
    12841 caggctggga aaacaccatt tccatactgg acaatatgcc ggaaattgaa gcgctggaca
    12901 atgccgttca gggtgagtgc aagataggta tccgcatagc ggcagaagaa gaacctaaat
    12961 ttgaattcta cacaagccgc ctgggtatcc gctacagcga tgttgtccct tattaccata
    13021 agtatattaa gcggaacaac aagttcaaat tgaaaatgct tcactttttc atcaacacgg
    13081 gcatgcagga caatgcatac tactggaacg aacttgcaaa atgtgtgaat gtgtattgtg
    13141 accttaagaa tttctgtcct gaactggact ccctgaacct gggtggaggt ttcccggtaa
    13201 ggaactccct atcgttcgat tacgattatg aatacatgac agaagagata attgcccaga
    13261 taaaaagtat ctgtacaagc cgcaacgtca aggaaccaga cattttcacc gagttcggat
    13321 ctttcactgt tggtgaaagc ggcgccaaca tataccaggt attaggtgaa aaacaacaaa
    13381 acgaccgcga aagatggtac atgatcgact catccttcat gactacattg cctgatacct
    13441 ggggcataaa aagacgcttc atactgcttc caatcaataa gtggaacgaa ccctatcagc
    13501 gcgtattcct tggaggactg acttgtgact ctcaggatta ctacaacgcc gaggcccacg
    13561 ccaatgcggt tttcctgccc aaaaacgaga acaaggatcc cttgtacatt ggttttttca
    13621 atacgggcgc ataccaggaa tccatttcag gtttcggggg tgtccagcat tgcctgatgc
    13681 cggctcccaa gatggtcata ataggagaag agaatggcac gtattacacc aagcttttct
    13741 ccaaggaaca gacttataag tccatgctca aacttctggg ctattgacaa catgttgagc
    13801 aaagccaaga tacaatacat taaagccctg gaacagaaga aattccgtca ggaacgacag
    13861 gaattcatcc tggaaggcag gaaaatggtt ctggagctgc ttaaagctcc tggtcccttc
    13921 cgcattaaag agctgtacct gcaggaagat ttccccgaac cggttcagtc ctccgtggca
    13981 gtatacatct cggcaaagga aatggaaagg atctccttgc agtcaactcc ctccggtgca
    14041 cttgctgttg tggagattcc aaaagtacca ctggcacctc cccggaaaga caacctatat
    14101 cttgcccttg acgggattcg cgatccgggc aattttggca ccatcctgcg tctggcagac
    14161 tggtttgact tagcagccgt ttatgcatca ccagactgtg tggagctgta caatcccaaa
    14221 accgtgcaat ccaccatggg agccattcta cgcgttccgg tccattacac accccttgcc
    14281 ccgcttttga aaaattgcac gcttcctgtc accgggtccg tgcttcaggg gggatccagc
    14341 atcaacacca ctttcctgcc ctgcgatgga atcatcgtcc ttggcaacga atcaaatggg
    14401 atcagcccgg agctgctgga tcacatcccg ctcaaattgt acattcctcc ttaccgcgag
    14461 ggatccgaat cattgaatgt ggctaccgct gctgcaatat tttgcgcagt tttccgtaga
    14521 aaccttccat ccctgcccag atagccaggt aaacaagaat aatacctgta ttgactgcaa
    14581 gtttgggaat catggacagg tgtgcaaaca taagggaaaa taagtaaagt cctacacctg
    14641 ccaccaggta gaaaccaatt ttaagccagt tataaggtat gggataatgt ttgcgtccca
    14701 tcagggcaca gaggacggtc attgacaggt aactggccaa gtgtccccag gctgcagcca
    14761 tatagccata gcgtggcatg aaaagcagat ttaccaacag ggtgacagcc agtccggctg
    14821 atgtgatcaa aacagcattt ccggtctttc ctgacaattt gaaccagatg ctgatattga
    14881 agtatatgcc cagaagcacg taagccagtg ccattacggg caccaccacg atcccctccc
    14941 tgtaattctc acctaaaatg agttcaaaaa ggtccatata aagtaaaata cccaggtaaa
    15001 ccagcatgca tattgctgta aagatatcca gcacacgggc atacgtcctt ggtgcttctt
    15061 tttcggctga ctgtccaaag aagaaaggtt ccgccgcgta ccgaaacatc tggatgaaaa
    15121 ggttaatgag tacggcgagc ttgatacccg cctggtaaat ccccatctgc gcccgccagg
    15181 agacatctcc aggaatcaga aaacggaaaa gcagcctgtc gctgaaatcg ttcaggatac
    15241 ccggcaatcc cgctatcatc aggggaaggg aatacatcat gagcttaggc cagagtttcc
    15301 ggtcaaggat gaaactgctt cttttcagca aatcaggcag gaaaagcaat aaagccacga
    15361 tgcaggagag gaatatggaa aagagaacgt aattgtaatc aggaacagcc gtgatgaaac
    15421 ggcccaggaa tgcttcgggg tttgcagaaa gataacgcgg ggcccacaaa aagaaaagca
    15481 ggttgaatcc cagctcgcag caaatcttga tggttttgaa tacggcaaaa cggaacgcct
    15541 tgtgctcgta acgcaaacgg gcaaacaata tagccgtaat gctatccagt a
//
LOCUS       DAOSOF010000033        15182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3936_length_15182_cov_6.734118, whole genome shotgun sequence.
ACCESSION   DAOSOF010000033 DAOSOF010000000
VERSION     DAOSOF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_15182_cov_6.734118"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLM68_03200"
     CDS             complement(<1..459)
                     /locus_tag="PLM68_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54405.1"
                     /translation="MNAGTLYGKYLNAEPQELVQQGAILQDLVRRYPWFSLGRLLLFK
                     SLCGLGGEASLSESEKTAAYVYSRSKLYFIRQESLQMEEQQAEIDFFTLDLTQEIEKE
                     EPSPKPATQKRREGKTTEFILEPPRDRTFYPGADYFGKTDMAGLELDVTEP"
     gene            complement(474..989)
                     /locus_tag="PLM68_03205"
     CDS             complement(474..989)
                     /locus_tag="PLM68_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOT54406.1"
                     /translation="MKKTGRISLRILALLLPLLLTRCWWYSFSGTSIHPQAETISVAY
                     IENRAERINPSLSNSLTEALMDKYTKLTRLSVIQDDGDYAVSGEITGYAIAPMAVTTD
                     EVASMTRLTVTVRIVFVNKLDPSENFDRNFSSYEDYDSSISFDAAEGGLVDTIVEKIV
                     EEIFNATVANW"
     gene            complement(986..2260)
                     /locus_tag="PLM68_03210"
     CDS             complement(986..2260)
                     /locus_tag="PLM68_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT54407.1"
                     /translation="MDVTAIKQRFQIIGTSPLLDRALDVAAQVASTDLSVLVTGETGV
                     GKEFIPQIIHAGSARKHGHYFAVNCGAIPEGTIDSELFGHLKGSFTGAHEDRKGYFEV
                     ADGGTLFLDEVAELPLSTQVRLLRALETGEFIRVGSSIVQKTDVRVIAATNVNLAHAI
                     KVGKFREDLYYRLNTVPILLPPLRERPQDIHLLFRKFAADFAEKYKMPVIRLAEDAQN
                     MLEQYRWPGNIRQLKNVAEQLSVLEQTRLVTAQALARYLPADSPLHLPVVFDSEKKEG
                     NDFISERDILYKILFQLRSEMDDMKQQIQNLAEQASLATGALGHAQGSTPPRAGTTSL
                     HVTGTGMHVKGSMLPHPAEDLGAAEEITEEELLPLETVEESRDLSLNKLEKELIMKVL
                     QKNKGHRKSTAKELGIAERTLYRKIKEYGIDS"
     gene            2360..4009
                     /locus_tag="PLM68_03215"
     CDS             2360..4009
                     /locus_tag="PLM68_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54408.1"
                     /translation="MKKTLLRYLGIAALGIMLMACNNPKDMVKYADDVEKSCDPEILE
                     VVADKIDVTYTLKFPDGYFHPKAMLEVTPVLVYEGGEVAAPALKLQGEKVLDNYNVIP
                     VAGEEIQHKLQFDYVDGMAKSHLELRGAVLYKDDVWNLPVAYKIADGANITYKWVELN
                     GEPVLMPDDYQKIIKEAEEVNIMYAKNSAQVRARELTKGEIKAFQDFLKNLPADQRRV
                     VTGTDIVAYASPEGPIDFNEKLSENRMKSAEKAFQTLTRKIETEGPLNLLTKGEDWAG
                     FQELVSKSNMPDKELILRVLSMYSDPMVREREIRNMSAVFKTLEEKILPELRRARLIA
                     NIEFTNYSDEELKELASTNMDALNEEALLYAASLVQDNDTKIALYKKAAEKYGSSRGQ
                     NNLAVAYLNGNKVNEAASALAKCDVSNPVVANNLGVIEMRKGNYGKAYEFFAKSGCPA
                     AKVNQGALDILNGKYKDALAKLDGQKHMNAALAQLLNGNLDKAEDALSECTCMKTTYV
                     KAVIAARRGQTSKVNELMEKINKVPALAAKAAKDIEFAKYR"
     gene            complement(4194..5981)
                     /gene="lepA"
                     /locus_tag="PLM68_03220"
     CDS             complement(4194..5981)
                     /gene="lepA"
                     /locus_tag="PLM68_03220"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT54409.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLELTHTVEQRDMEAQVLDSM
                     DLEREKGITIKSHAIQLDYQHNGEQYVLNLLDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGVQAQTISNLYLAISHSLEIIPILNKIDMESAMIDVVQDQIIDLLGCKPGEILHI
                     SARTGEGVQQVLDAIVERIPSPKGDPEAPLQALIFDSVFNSFRGIIAYFRVMNGTIGQ
                     GDKVKFFNTGKVYEADEVGILKMKMIPRKEISTGDVGYIISGIKTVSEVKVGDTITHV
                     ARPCQKAIAGFEEVKPMVFAGLYPVDTDGYEDLRTALEKLQLNDASLTFEPESSLALG
                     FGFRCGFLGLLHMEIVQERLFREFDCDVITTVPNVSYKVYTTQGNVADVHNPSGLPAL
                     TIIDHIEEPYIKAQVISKSEYFGAIMKLCLDKRGVLANQHFLTADRVELTFRMPLSEI
                     VFDFYDKLKSISRGYASFDYHIIGHERADLVKLDILLNGEPVDALSSLIYRGHSYDFG
                     RKMCEKLKELIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKRRMRQIGNVEVPQSAFMAVLKLD"
     gene            6041..6886
                     /locus_tag="PLM68_03225"
     CDS             6041..6886
                     /locus_tag="PLM68_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT54410.1"
                     /translation="MNKKIPYPVSDIIAQIRRDIIRMVTAAKSGHPGGSLSSADFMTV
                     LFAEFLEQDPQQWKRNGKDMDMFFLSIGHVSPLFYSLLARMGYFPVQELASFRKFGSR
                     LQGHPCVEYGLPGVHVASGSLGQGLSAASGAALSKRLAGDDHKVYVLMGDGESEEGQV
                     WEAALFAAHHNAGNLTAITDWNRQQIDGPVNVIAGLGDLVAKWEAFGWMVLECDGHNL
                     NAIRDAFRESLKPRSKPMMILMHTNMGQGVDFMTGSHAWHGKAPSAEQEALALSQLPE
                     TLGDY"
     gene            6899..7834
                     /locus_tag="PLM68_03230"
     CDS             6899..7834
                     /locus_tag="PLM68_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT54411.1"
                     /translation="MTNTGNKDTRSGFGAGLYELGSRNPSVVALCADLTGSLKMEAFA
                     EAFPQRFFQCGIAEANMMGVAAGMAVTGLIPFVGTFAEFATGRVYDQLRQSVCYSQTN
                     VKIAASHAGVTLGEDGATHQTMEDLALARALPHMTVINPCDYNQTRLATIAAASWEGP
                     VYLRFGRPSVPNFSPADGLFEIGKAWMIQPGEDVTILATGHLVWEALEAVRVLARKGI
                     RAELINIHTLKPLDEQAILASARKTGAVVTAEEHLLAGGLGELVSGLLARHYPVPMRM
                     VAMGDGFGQSGTPRELMDNYGLNTAGIIEKVCELR"
     gene            7821..8348
                     /locus_tag="PLM68_03235"
     CDS             7821..8348
                     /locus_tag="PLM68_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT54412.1"
                     /translation="MNSDKELKVLCESGQYENAFALIVQEYTQRLYWHIRKMVLLHDD
                     ADDVLQNTLLKAWTGLPTFRWESQLFTWLYRIATNEVLTFLRKSKSANQMPLEERLDA
                     DPEYFNGDQLQKALQQAILRLPPKQRLVFNMRYFDEIRYQDMSRILGTSEGALKASYH
                     LAAAKVEEILKELLL"
     gene            8384..8869
                     /locus_tag="PLM68_03240"
     CDS             8384..8869
                     /locus_tag="PLM68_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54413.1"
                     /translation="MNEKTLHNPHLKENPFRVPDGYFEQVQARWAQKTVDEQSGRSPE
                     VFPEKHDEGRFRRIIRLLKPQMQLAAGFVLLFGIGSLMMYITSRNQKTEAQTAEFSLM
                     SELSYWGVDHRVVSDLILEDFPDEDNAYVLTEMDVEELIDYLNYPSLDLDEYDNYTNN
                     E"
     gene            8880..9476
                     /locus_tag="PLM68_03245"
     CDS             8880..9476
                     /locus_tag="PLM68_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54414.1"
                     /translation="MKRSIIIFFLCFVTLPAFSQEVRVHKSEQERKAEREAVQSQKIA
                     FITQEVGLTPAEAEKFWPLYNEMERKVREVVHKRNNAKAEMYKLFASDEREKKGSGVE
                     PAGYSQGRTAAGDQRDAAEIAMVDNKPKGPAAPVEDLLASYMNSFQEENTVRMEYHQK
                     FLHIMPAAQVARFYLAEERFSNKMLRQYIENKVQEKSR"
     gene            complement(9477..10202)
                     /locus_tag="PLM68_03250"
     CDS             complement(9477..10202)
                     /locus_tag="PLM68_03250"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT54415.1"
                     /translation="MEPFRFKQFTLSHGRSAMQVNTDSVILGAWAAGASQAPGRILDI
                     GTGCGILALMMAQRFSGTMIDALEIDKSSAEEAAANALSSPWAGRISVRQGDFRKTVF
                     TGRYDLVICNPPYFRKSLASPDLRRTIARHASPSSLSHRELSEGVADLLAPSGLFALV
                     LPATAAGDFFSIALAAARPLYPQRVTTVYTRMSKPAIRVLAEMGHTPVPAVRDTMAMY
                     EESGPEYSSQYVSLVKDFYLWAQ"
     gene            complement(10204..10857)
                     /locus_tag="PLM68_03255"
     CDS             complement(10204..10857)
                     /locus_tag="PLM68_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT54416.1"
                     /translation="MVKADYITKSFGNVQVLKGISLEVAPAEMVCITGPSGAGKTTLL
                     QILGTLSPPDSGQVWIDGTPVCELKKDKLADFRNRHIGFVFQFHHLLPEFTALENVCI
                     PGFIAGDAAEKVQARAKELLDFLGLAHRMEHKPAELSGGEQQRVAVARALINSPGILL
                     ADEPSGNLDTHNKEELHRLFFRLRDTFGQTIIMVTHDQSLATRSDRTLNMRDGLFLQ"
     gene            complement(10875..13028)
                     /locus_tag="PLM68_03260"
     CDS             complement(10875..13028)
                     /locus_tag="PLM68_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT54417.1"
                     /translation="MKTYTTEQIRNMVLMGNTKSGKTTLAEAMMMEGKVIDRRGTVEA
                     KNTVCDNTEIEQLYQRSIYPTLFYTEYADHKLNFIDTPGSDDFIGGLISAFRVADSGV
                     MLINAQQGVEVGTEIFSRYAEKYNKPLFLAISQLDHEKANWEACLDSLHEAYGNKVCP
                     IQFPVDTGLNFNAFVDVLSMKMYRFKDDSGTREELDVPADLAGKAETMYAELTELAAE
                     AEEKLMEAFFENGTLTPEQIIQGLIIGFRKCEIMPLVIVSGKKDIGVKRLLELITQIA
                     PHPGLTKSKTVSGEEVPCDVNGAPSLYIYKTSQEQHLGDIAYFRVMSGKITESIDLVN
                     ANNGSKERISGLFIAAGKKREKVTELLAGDMGCTVKLKNAKSNHTLNGNGKSWEFEPI
                     AFPPAKFRTAIKAKSEKDDEKLGEILNKASAEDPTIVVEYSKELRQIIVSGQGEHHIN
                     TLKWHINNTYKMEVDLIAPKIPYRETITKMAASSYRHKKQSGGAGQFGEVHLLIEPYI
                     EGAPDNTRFKVDGKDMVLSIRNHDEYTMDWGGKLIYNNCIVGGSIDGRFMPAILKGIM
                     EKMEEGPLTGSYARDIKCYVYDGKMHPVDSNEISFKLAGRNAFKDAFKKASPKIMEPI
                     YMVEVMVPSDCMGDVMSDLQNRRALIEGMSSEKGFQILKARVPLAELNKYSTTLSSLT
                     SGRASFTMTFLEYTQVPSDVQERLLKEYEEEEKDE"
     gene            13106..13723
                     /locus_tag="PLM68_03265"
     CDS             13106..13723
                     /locus_tag="PLM68_03265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT54418.1"
                     /translation="MYFPLLPHLEAGRLEAGCDEAGRGSLAGPVYAAAVILPPGFSHS
                     LLRDSKMMSEKQRLEVRSVILEKAIAWAIAWCSPEEIDRWNILRASITAMHRALDKLS
                     TPPSFILVDGNRFYPYHRGGGDALLTPVIPHQCFVRGDDRFASIAAASVLAKTFRDEH
                     MAVLDSRFPQYGWKDNKGYPTVKHREAIRDFGLTEHHRKSFSYHL"
     gene            13774..>15182
                     /locus_tag="PLM68_03270"
     CDS             13774..>15182
                     /locus_tag="PLM68_03270"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOT54419.1"
                     /translation="MKKLSFILCTVLLLGGNVRADEGMWLLPLLNQLNNKAMKDAGLR
                     LDAEDIYNINRSSLKDAIVIFGGGCTGEVVSPEGLLLTNHHCGYGMIQTLSSVEHNYL
                     RDGFWARNREEEIPAPGLRVTFMESMRDVTKEVLAVLDTCSTEESRSDTEFRLGREWS
                     EKIRKENPRFSGRLYSMYGGNQYFLVVYKVYTDVRFVGAPPSSIGKFGADTDNWMWPR
                     HTCDFSMFRIYAGPDNEPADYAPENRPYKAKYSLPISLKGVQQGDFVMTLGYPGTTTR
                     YMTSIEVDRQEQLTNNIGIEVRTIRQNILLEDMLADPKVQLQYASKYSGSTNAWKKWT
                     GMNETFGKLNVKARRAAEEAAFTAWVNASKKRIEKYGTALKDIEEAIQGTWDNLYVSR
                     YLSEALRNIELASFASRVARLVSRPGEDGSVDTEGLKAQAERFFINYNAPTDLKVAKA
                     MLKHFALKVDKAYYPSVYG"
BASE COUNT         3642 a         3861 c         3954 g         3725 t
ORIGIN      
        1 aggctcagta acatccagtt ccaagcccgc catgtcggtt ttcccgaaat agtctgctcc
       61 cggataaaag gtgcggtctc ggggtggttc aaggataaac tcggtggttt ttccttccct
      121 gcgtttttgt gttgccggtt tcggggaggg ctcctccttt tcaatttcct gtgtcaggtc
      181 cagtgtgaag aaatcgatct cagcctgttg ctcttccatc tgcaggctct cctgccggat
      241 aaagtaaagt ttgctgcggg aataaacgta ggcggctgtt ttttcagatt cggagaggct
      301 ggcctcccca cccaggccgc aaagcgattt gaacagtaat aggcgtccca gggaaaacca
      361 gggataccgc cgcaccagat cctgcaggat ggctccctgc tggaccagtt cctggggttc
      421 cgcgttgagg tattttccgt ataatgtccc ggcgttcatt ggtgtgtttg ttcttaccag
      481 ttggcaactg ttgcgttaaa aatttcttcc acgatctttt ccactatagt gtctaccagg
      541 ccgccttctg cggcatcaaa agaaatggaa gagtcatagt cttcatagga tgaaaaattc
      601 ctgtcaaaat tctcggaagg gtccagcttg tttacaaaca cgatcctgac cgtgaccgtc
      661 aggcgtgtca tggaagcgac ttcatctgtg gtcaccgcca tgggcgcgat ggcatatccc
      721 gtgatctcgc cggacaccgc ataatccccg tcgtcctgta tcacggaaag ccgggtcagc
      781 ttggtgtatt tgtccatgag cgcctcggtc agggagttgc tcagggaggg atttatgcgt
      841 tctgccctgt tctctatata ggcaacgctg atggtctctg cctggggatg aatggaagtg
      901 cccgaaaagg agtaccacca gcaccgggtg agcagtaaag gtaaaaggag tgcaaggatt
      961 ctcaaggata tgcgtcctgt ctttttcatg aatctattcc gtattctttg attttccggt
     1021 acagggtccg ctctgcaatg cccagttctt tggctgtaga tttgcgatgg cctttatttt
     1081 tttgcaagac tttcattatc agttcctttt caagtttatt gagcgaaagg tccctgcttt
     1141 cctcaacggt ttccagggga aggagttctt cctctgttat ttcttccgca gcaccgagat
     1201 cctctgccgg atggggcagc atgctccctt tcacatgcat gcctgtccct gtgacatgca
     1261 aagacgttgt tccggctcgt ggcggggtac ttccctgcgc atgtcccagc gctcccgttg
     1321 ccagggatgc ctgttcagcc aggttttgga tttgctgttt catatcatcc atttccgacc
     1381 gcagctggaa aaggattttg tacaggatgt ccctttcaga aatgaaatca ttgccttctt
     1441 ttttctcgga atcaaatact accggcaggt gaagcgggct gtctgcggga aggtaccggg
     1501 caagggcctg ggcggtgacc agccgtgttt gttccaggac gctgagttgt tctgccacat
     1561 tctttagctg gcgtatgttt ccgggccagc ggtattgctc cagcatattt tgagcatctt
     1621 ctgccagccg gatgacgggc atcttgtatt tttcggcaaa gtcggctgcg aattttcgga
     1681 acagcaggtg gatgtcctga ggcctttccc gaagtggggg caacagtatt ggaaccgtat
     1741 tcagcctgta atacaggtct tcccggaatt ttcccacctt gatggcgtgg gcaaggttca
     1801 cattggtggc ggcaatcacc cgcacgtctg ttttctggac tatggaagat cccacccgaa
     1861 tgaattctcc tgtctccagg gcgcggagga gacgcacctg cgtggaaagg ggcagttcgg
     1921 ccacttcgtc caggaacagc gttcccccgt cggcgacctc gaagtatccc ttccggtctt
     1981 catgggcccc ggtgaacgat cctttcaggt gcccgaacaa ttcagagtct atagtccctt
     2041 cgggaatcgc gccacagttg acggcaaaat aatggccgtg tttgcgggcg cttccggcgt
     2101 gtatgatctg gggaataaat tccttgccca cacccgtttc accggttacc aaaaccgaaa
     2161 ggtcggtgga ggccacctgt gccgccacat caagggcacg gtccagcaaa gggcttgtcc
     2221 ctattatttg aaatcgctgt tttatagctg taacatccat ctttgcaaag atataaattt
     2281 tgtactatat ttgtaagcta aaccaacagt gttaaatcat tagattaacg ttaaaatacc
     2341 aaataataaa ctactcgtta tgaaaaaaac tttattgcgc tatctgggca ttgccgctct
     2401 tggcataatg cttatggctt gtaacaatcc aaaggacatg gttaaatacg ccgatgacgt
     2461 tgaaaaatca tgtgatccgg aaattcttga agtagtcgca gataagattg atgtgactta
     2521 taccttaaaa tttcccgacg ggtactttca tcccaaagca atgctggaag tcactcccgt
     2581 attggtatac gaaggcggcg aggtagctgc tcctgcgctt aagctccagg gagagaaagt
     2641 gctggataac tacaatgtca ttcctgtagc cggggaagaa atccaacaca agctccagtt
     2701 cgattatgtt gacgggatgg caaaatccca cctggaattg cgtggtgcag tcctgtataa
     2761 ggacgatgtc tggaatcttc ccgttgccta taagattgct gacggagcca acatcactta
     2821 caaatgggtg gaactcaacg gagaacctgt cctgatgccc gacgattacc agaagatcat
     2881 aaaagaagca gaagaagtta atatcatgta tgccaagaac tccgcccagg tccgggcccg
     2941 cgagcttacc aagggtgaga tcaaggcatt ccaggatttc ctgaaaaacc ttcctgccga
     3001 tcaaagaagg gttgtcaccg gaacagacat tgttgcctat gcttcccccg aaggcccgat
     3061 agatttcaac gaaaaacttt ccgagaatcg tatgaaaagt gccgaaaagg cattccagac
     3121 actgacccgc aaaattgaga ctgaaggtcc gctcaacctg ttgaccaaag gcgaagactg
     3181 ggcaggtttc caggaattgg taagcaaaag caacatgcct gacaaggaac tcatcctgcg
     3241 cgttctgtcc atgtactccg accctatggt ccgcgagcgt gagatccgca atatgtctgc
     3301 tgttttcaaa acactcgaag aaaagatatt gcccgaactg cgtcgcgcac gcctgattgc
     3361 caatattgag ttcaccaact attccgatga ggaactgaaa gagctcgcat ccaccaacat
     3421 ggatgccctg aatgaagaag cccttttgta tgccgcctct ctggtccagg acaacgacac
     3481 caagatcgcc ctgtacaaga aggctgcaga gaaatacggc tcttcaaggg gtcaaaacaa
     3541 tttggcagta gcttacctca acggcaacaa agtcaatgaa gccgcctcgg ccctggccaa
     3601 atgcgatgtt tccaatcctg tggtagccaa caacctgggt gttattgaaa tgcgcaaagg
     3661 caactacgga aaagcttatg agttctttgc aaaatccgga tgccccgctg caaaagtcaa
     3721 ccagggcgcc ctggatatcc tcaatggcaa atacaaagat gcccttgcca aactggacgg
     3781 ccaaaaacac atgaacgccg ccctggcaca attgctcaac ggcaacctgg ataaagccga
     3841 ggatgccctg tccgaatgca cctgcatgaa aaccacctat gtaaaagccg ttatcgctgc
     3901 ccgcagggga cagacctcga aggtcaacga gctgatggag aagatcaaca aagttcccgc
     3961 tctggccgcc aaagctgcaa aagacattga atttgcaaaa tatcgttaaa cgacccactt
     4021 aacctcacac cttacagaga agaggctgac cggttggtca gtctcttttt tcagggtggc
     4081 aaacccggag tgccgcgcaa gagggatttg gcgttttgca tgccgctgat attctgatat
     4141 ttatatttca acaaattggc attgcgcggt aaaccgggtt tgccctgcgc aagctaatcc
     4201 agtttcaaga cggccatgaa agctgattga gggacctcca cattgcctat ctggcgcatg
     4261 cggcgtttcc ctttcttttg tttttcgagg agtttgcgct tgcgggtgat gtcacccccg
     4321 taacacttgg cggttacgtc cttgcgtacc gccttgacgg tttcgcgggc aatgatcttt
     4381 gccccgatgg ctgcctgtat ggctatgtcg aattgctggc gcgggatgag ctctttcagt
     4441 ttttcacaca ttttcctccc gaaatcgtag gagtggccac ggtagataag gctggacagg
     4501 gcgtcaaccg gttcgccgtt caacagaatg tccagtttga ccaggtcggc acgctcatgt
     4561 cctataatgt ggtaatcaaa agaggcatag ccacgggaaa tgctcttgag cttgtcataa
     4621 aaatcaaata ctatctctga aagcggcatc ctgaatgtaa gctccacccg gtctgccgtc
     4681 aagaaatgct ggttggccag gacacctcgt ttatcaaggc agagtttcat gatggccccg
     4741 aagtattctg acttggagat cacctgtgcc ttgatgtagg gttcttcaat gtggtctatg
     4801 atggtaaggg ccggcagacc tgaagggttg tgcacatcgg caacgttccc ctgggtggtg
     4861 taaaccttgt aggatacgtt gggcacggtg gtgatcacgt cgcagtcaaa ttcgcggaac
     4921 agcctttcct gaacaatctc catgtgcaac agtcccagga acccgcaccg aaaaccaaat
     4981 cccagggcca gggacgattc cggttcaaag gtaagcgagg catcgttgag ttgcagcttt
     5041 tcaagggctg ttctcaagtc ttcatatccg tccgtatcca cagggtacag cccggcaaag
     5101 accatgggct tgacttcttc aaagccggca atggcctttt ggcagggacg ggccacatgg
     5161 gtgatggtat cccccacttt cacttccgaa acagtcttga tacccgagat gatatacccc
     5221 acatctccgg tgctgatctc cttgcgtggg atcatcttca ttttcagaat ccccacttcg
     5281 tcggcctcgt agacttttcc cgtattgaaa aacttcacct tgtctccctg ccctatagtc
     5341 ccgttcatga ccctgaaata ggcaatgatg ccgcgaaagg aattgaagac ggaatcaaag
     5401 atgagcgcct gcaggggagc ctccggatcg cctttgggcg aagggatgcg ctctacaatg
     5461 gcatccagca cctgctggac accttcccct gtacgggcgc taatgtgcag gatttcaccg
     5521 ggcttacatc ccagcaggtc aattatctga tcctgtacca catcaatcat ggcagattcc
     5581 atgtcaattt tgttcaggat ggggatgatc tccaggctgt gtgatatggc caggtacaga
     5641 ttggatatgg tctgggcctg taccccctgg gtggcatcca ccaccagaag ggctccttcg
     5701 cacgaggcta tggcccgcga aacctcatat gaaaaatcaa cgtgccccgg ggtgtccagc
     5761 aggttgagca cgtattgctc tccgttgtgc tggtaatcca gctgtatggc atgagacttt
     5821 atagtaattc ctttttccct ttccaggtcc atactgtcca atacctgtgc ttccatatcg
     5881 cgttgctcca cggtatgggt gagctcaaga agacggtcag ccaaagtgct ttttccgtga
     5941 tctatatggg caataatgca gaaattcctg atgtttttca taagtggggc aaaggtaaat
     6001 ataattttta tctttgtagg ataatcaaat gaaggacaac atgaacaaga aaattcctta
     6061 ccctgtttca gatatcatag cacagatccg ccgggatata atccggatgg taacggcagc
     6121 aaaaagcggt cacccgggag gctctctgag cagcgcggat ttcatgacgg tgctttttgc
     6181 cgaattcctg gaacaggatc ctcaacagtg gaaacgcaac ggaaaggaca tggatatgtt
     6241 ctttctttcc attggtcatg tttctccctt attttacagc ctgctggccc ggatgggtta
     6301 ttttcccgtc caggaactgg cttctttccg caaattcgga tcccgcctgc agggtcatcc
     6361 ctgcgtggag tacgggttgc ccggtgtcca tgtagccagc ggatcgctgg gacagggtct
     6421 ctcggcagcg agcggcgcag cactgagcaa acgactggcg ggcgatgacc acaaagttta
     6481 cgtacttatg ggcgacggtg aaagcgaaga ggggcaggtg tgggaagcag ccttgttcgc
     6541 tgcccatcac aatgccggga acctgacagc cattacagac tggaaccggc agcagattga
     6601 cggaccggtc aacgtgattg ctggattagg tgacctggtc gccaaatggg aggctttcgg
     6661 gtggatggtg ctggaatgtg acggccataa cctcaatgcc ataagggatg cgttcaggga
     6721 gtcgctcaaa ccacgtagca aacccatgat gatccttatg cataccaata tggggcaagg
     6781 cgtggatttc atgacagggt cccatgcctg gcacggaaaa gcgccttctg cagaacagga
     6841 agcccttgcc ctgtcacaat tgcctgaaac actgggagat tattaacaga ttacaactat
     6901 gacaaataca ggaaataaag atacccgttc cggttttggt gccggactgt atgaattggg
     6961 gagcaggaac ccttcagttg tggctttgtg cgccgacctg accggttcgc tcaaaatgga
     7021 ggcgtttgcc gaagccttcc cgcagcgttt ttttcaatgc ggcatagccg aggccaatat
     7081 gatgggggtg gccgccggca tggcggtgac cgggctgatc ccctttgtgg gaacttttgc
     7141 cgaatttgcc acgggacgtg tttacgacca gctgcgtcag tccgtctgtt attcgcagac
     7201 aaatgttaaa attgccgctt cacacgccgg agtgaccctg ggagaagacg gtgcaaccca
     7261 ccagaccatg gaagaccttg ccctggccag ggcccttccg cacatgaccg tgatcaatcc
     7321 atgtgattac aaccagaccc ggcttgccac aatagcggcc gccagctggg aaggaccggt
     7381 ttacctgcgg tttggaagac cctctgttcc caacttcagc cccgctgacg gacttttcga
     7441 gataggaaaa gcctggatga tccagccggg cgaagacgtg accatccttg ctaccggaca
     7501 cctggtctgg gaagccctgg aagctgtcag ggtgcttgcc cgaaagggga tccgtgccga
     7561 gctgataaat attcacaccc tcaaacccct ggatgaacaa gccatcctgg cctcggcccg
     7621 taaaaccggc gcggtggtga ccgcagaaga acaccttctg gccggaggac tgggagagct
     7681 ggtctcgggc ctgctggccc gtcactatcc cgttcccatg cgcatggtag ccatgggtga
     7741 cggttttgga caaagcggca caccccgtga actgatggac aattacgggc tgaacactgc
     7801 cggtatcatc gaaaaagttt gtgaactcag ataaggaatt aaaagtctta tgcgaaagcg
     7861 gtcagtacga gaacgccttc gccctgattg tgcaggagta cactcaacgc ctgtattggc
     7921 atatccggaa gatggtgctc ctgcatgacg atgccgacga tgtcctgcaa aacaccctgc
     7981 tcaaggcctg gacaggattg cccacattcc ggtgggaatc tcaactcttt acctggcttt
     8041 accggattgc cacaaacgag gtcctgactt tcctgagaaa gagcaaatcc gcaaatcaaa
     8101 tgcccctgga agaacggctg gatgcagacc cggaatattt caacggcgac cagttgcaga
     8161 aagccctcca gcaagccatc ctgaggctgc ctccaaaaca gcgtcttgtt ttcaacatgc
     8221 gctattttga cgagatccgc taccaggaca tgtcgcgcat cctgggcaca tccgagggag
     8281 ccctgaaagc ctcctaccat cttgctgcag ccaaagtaga ggaaatcctc aaagaattgc
     8341 tgctttaaac ttttgtaccc gcacgccatc taacagttga aacatgaacg aaaaaacgtt
     8401 acataaccca cacctgaaag agaatccctt ccgggttccc gatggctact ttgaacaggt
     8461 acaagcccgc tgggcccaaa agactgttga tgaacagtcc ggccggtcgc cggaggtttt
     8521 ccctgaaaaa cacgatgagg gcaggttcag gcgcatcatc cggctgctaa agccccagat
     8581 gcaacttgcc gcaggttttg tgttgctttt tgggatcggg tccctgatga tgtacataac
     8641 cagccggaac caaaaaacag aagcacagac ggcggaattc tccctcatga gcgaattatc
     8701 ttactggggg gtggaccacc gggtggtttc cgatcttatc ctggaagatt ttcccgatga
     8761 agacaacgcc tatgtcctga cagaaatgga tgtagaggag cttattgatt atttgaatta
     8821 tccgtcccta gacctggatg aatatgacaa ctacacgaac aatgaataaa acaaacgcta
     8881 tgaaacgttc aatcataata ttctttcttt gctttgtaac ccttccggcc ttttcccagg
     8941 aagtccgtgt gcacaaaagt gaacaggaac gcaaggctga acgtgaagcc gtccagtctc
     9001 aaaaaatcgc atttattacc caggaagtgg gactgacccc cgcagaagcc gaaaaattct
     9061 ggcccctgta caacgagatg gagcgcaagg tgagggaggt ggtacacaaa cgtaacaatg
     9121 ccaaggctga aatgtacaaa ctgtttgcct ctgatgaacg ggagaagaag ggaagcggtg
     9181 tcgaaccggc aggctattca cagggcagga ctgcagccgg cgatcagcgg gatgcggcag
     9241 agatcgcgat ggtggataac aagcctaaag gacccgcggc ccccgttgag gatctgctgg
     9301 cctcctatat gaattcgttc caggaagaga ataccgtaag gatggaatac caccagaaat
     9361 tcctgcacat tatgccggct gctcaggttg cccgttttta cctggcagaa gaacgtttca
     9421 gcaacaagat gttacgtcag tatatagaaa acaaggtgca ggaaaagtcc agatagttac
     9481 tgcgcccaca aatagaaatc ctttaccaga gacacatact gcgagctgta ttccggcccg
     9541 ctttcttcat acatggccat cgtgtccctg acagcgggaa cgggagtgtg gcccatttcg
     9601 gcaagaaccc ttatggcagg cttgctcatc ctggtataaa cggtggtgac ccgctgagga
     9661 taaaggggcc tggcagcagc caatgctatg gaaaagaaat cccctgcagc cgtggcaggg
     9721 aggaccaggg caaaaagacc ggatggtgcc agaaggtccg ccacaccttc cgacagttcc
     9781 cggtgtgaca ggcttgacgg ggaagcgtgc cgggcaatgg tgcggcgcag atcgggactt
     9841 gccagggact ttctgaagta aggaggattg cagatgacaa gatcataccg gccggtaaag
     9901 actgttttac ggaaatcacc ctgtctgacg cttatcctgc ctgcccatgg agaagacagg
     9961 gcgttggcag cagcctcctc cgcagaagac ttgtcgattt ccagggcatc tatcatggtg
    10021 ccggaaaatc gttgcgccat catcagggcc aggataccgc acccggtacc tatgtccagt
    10081 atcctgcccg gtgcctgtga agcacctgca gcccaggctc ccagtatcac actgtctgtg
    10141 ttgacctgca tggcggaacg gccatgggac aaagtaaatt gtttgaaacg gaaaggttcc
    10201 acgctattgg agaaatagcc cgtcgcgcat gttcagggtc ctgtcactcc tggtggccag
    10261 tgactgatcg tgcgtgacca tgatgatggt ttgaccgaag gtgtcccgca agcggaaaaa
    10321 caagcggtga agttcttcct tgttatgggt gtccaggttc cctgaaggtt cgtcagccag
    10381 caggattccc gggctgttga taagggctct tgccacggca acgcgttgtt gttctccccc
    10441 gctcagttct gccggtttgt gttccatgcg gtgggccaga cccagaaaat ccaggagttc
    10501 ctttgccctg gcctggactt tttctgctgc atcaccggct atgaaaccgg gaatgcacac
    10561 gttttccagg gctgtgaatt cggggagcag atggtggaac tggaaaacaa agcctatatg
    10621 ccggttgcgg aaatcggcaa gcttatcctt tttcaattca cacacagggg tcccgtcaat
    10681 ccatacctgg ccggaatcgg gtggggaaag tgttcccagg atttgcagta acgtggtctt
    10741 ccccgccccg ctggggccgg tgatacaaac catttcggca ggtgcaactt caagggatat
    10801 ccccttcaga acctgcacat tgccgaatga tttggttatg taatccgctt tgaccatgtg
    10861 ttgcggaaac aggattattc gtctttttcc tcttcttcgt attctttcag gagcctctcc
    10921 tggacatcgg aaggaacctg cgtgtactca aggaatgtca tggtaaagct tgctcggccc
    10981 gaggtaaggg agctgagcgt tgtggagtat ttgttcagct cggccagggg cacccttgcc
    11041 ttgagtatct ggaatccctt ttcactgctc attccctcta taagggcacg gcggttctgc
    11101 aggtcgctca tcacatctcc catgcagtcc gaggggacca tcacttctac catgtaaatg
    11161 ggttccatga ttttgggcga tgcctttttg aaagcatcct tgaaggcatt ccgccccgcg
    11221 agtttgaaag atatttcgtt ggaatccacc gggtgcatct ttccgtcgta cacataacac
    11281 ttgatgtcgc gggcataaga cccggtcagg ggtccttctt ccattttctc catgatcccc
    11341 ttgaggatgg ccggcatgaa acgtccgtca atggaaccgc ctacgataca gttattgtag
    11401 atgagctttc cgccccagtc catggtgtat tcatcatgat tccttatgct caggaccatg
    11461 tccttcccgt caaccttaaa gcgtgtatta tcaggtgctc cttcaatata gggctcaatg
    11521 agaaggtgca cttcgccaaa ctggcctgcc ccacccgatt gttttttatg acggtagctc
    11581 gaggcggcca tttttgtaat ggtctcgcgg tacgggattt ttggggcgat caggtcaacc
    11641 tccatcttgt aggtgttgtt gatgtgccac ttgagtgtat tgatgtggtg ctcaccctgt
    11701 ccgctgacaa tgatctgcct cagttccttg gagtattcca ctacaatggt gggatcttct
    11761 gctgaagcct tgttcaggat ctcgcccagt ttttcgtcat ccttttctga ttttgccttg
    11821 atggcggtac ggaattttgc gggtgggaaa gcgatgggtt caaattccca gcttttgccg
    11881 ttcccgttga gtgtgtggtt tgatttggcg ttttttagct tgacggtgca gcccatgtct
    11941 ccagccagaa gttctgtgac tttttcgcgt ttctttccgg ctgctataaa gagacccgag
    12001 atcctttcct tgctgccgtt gtttgcgttg accaggtcaa tggattctgt gatcttgccc
    12061 gacatcacac ggaagtaggc aatatccccc aggtgctgtt cctggcttgt cttgtagata
    12121 tagagagagg gggcgccatt cacgtcgcag ggcacctctt caccggaaac agtcttgctc
    12181 tttgtcagtc ccggatgggg ggcgatctgt gtgatgagtt ccagcagacg tttcaccccg
    12241 atgtcttttt taccggaaac aatgaccagg ggcatgatct cacacttgcg gaaccctatg
    12301 atcagaccct gtatgatctg ttccggagta agtgtgccgt tctcgaaaaa ggcttccatg
    12361 agtttttctt ccgcttcggc ggccagttct gtcagttctg catacatggt ttcggctttg
    12421 ccggccaggt ctgcaggaac gtccagttct tcgcgggtac cactgtcgtc tttgaagcga
    12481 tacattttca tggaaaggac atccacaaag gcgttgaaat tcaaacccgt atctaccggg
    12541 aactggatgg ggcacacctt gttgccatag gcctcgtgca aagaatccag gcaggcttcc
    12601 cagttggctt tttcatggtc aagctgactg atggccagaa aaagcggttt attgtacttt
    12661 tctgcataac gcgagaagat ctcggtaccc acttccacgc cttgctgggc gttgataagc
    12721 ataacgcctg agtcggccac cctgaatgca gatatcagac cgccaatgaa atcgtcggag
    12781 cccggggtat caatgaaatt caatttatga tctgcatatt ccgtgtagaa tagtgtagga
    12841 taaatcgatc tttgatacag ttgttcaatt tccgtattgt cgcacacggt attcttggct
    12901 tccacggttc cacggcggtc aatcaccttg ccttccatca tcatggcttc cgcaagggtg
    12961 gttttgccgg acttggtgtt ccccatcaga accatgttgc gaatttgttc tgttgtataa
    13021 gttttcatgc tatctgactg tttgtttatt tactggatat tacaaggcga caaaggtaat
    13081 aattttttct tttacctttg tacatatgta tttccctttg ttaccgcatc ttgaggctgg
    13141 cagattggag gccggatgtg atgaggcagg caggggatcg ctggccggcc ccgtgtatgc
    13201 tgccgcggtt atccttccac ccggtttcag ccactccttg ctgcgggatt caaaaatgat
    13261 gagcgagaaa caacgtcttg aggtgcggtc cgtcatcctg gaaaaggcta tcgcctgggc
    13321 tatcgcctgg tgttcccctg aggaaattga cagatggaac atactccggg cctccattac
    13381 ggccatgcac agggcactgg acaaattgtc cacaccaccc tctttcattc tggtggacgg
    13441 gaaccgtttt tacccctacc atcgcggcgg cggggacgcg ttgttgaccc cggtgatacc
    13501 gcaccagtgt tttgtcaggg gtgatgaccg ttttgcctcc atcgccgccg cctcggtgct
    13561 ggccaaaaca ttccgggatg aacacatggc ggtactggat tcacggtttc cacaatacgg
    13621 atggaaggac aataaaggat accccaccgt caaacacagg gaagccataa gggatttcgg
    13681 cctgacggag caccacagaa aatcattttc ttatcattta taattacctt tgcaggttaa
    13741 atatccgaaa cacaaaacac aaagaaatta tccatgaaaa aactgagttt tatcctgtgc
    13801 actgtgctgc tcctgggcgg caacgtacgt gcagacgagg ggatgtggct gcttccgctc
    13861 ctgaaccaat tgaataacaa agccatgaaa gatgccggtc tcaggcttga tgctgaagat
    13921 atctacaaca tcaaccgttc cagcctgaaa gatgccatag ttatatttgg cgggggttgt
    13981 accggagagg tcgtttctcc ggaagggttg ctcctgacaa accaccattg cggctacggt
    14041 atgatccaga ccctgagcag cgtggaacac aattacctga gggatggttt ctgggcaagg
    14101 aacagggaag aggaaatccc ggcccccgga ttgcgggtta cattcatgga aagcatgcgt
    14161 gacgtgacca aagaagtcct ggcggtgctg gatacctgtt ccacggaaga aagccgctca
    14221 gacacagaat tccgccttgg aagagagtgg agcgaaaaga taaggaaaga gaatccacgg
    14281 tttagcggac gcctgtacag catgtacggg ggcaatcaat atttcctggt tgtttacaaa
    14341 gtatacacag acgtgcgctt tgttggtgct ccgccgtctt ccatcggaaa attcggcgcc
    14401 gacacagaca attggatgtg gccccgccac acgtgtgact tttccatgtt ccgcatttat
    14461 gccggtccgg acaatgagcc ggcagactat gctccggaaa accgccctta caaggcaaag
    14521 tattccctgc ccattagtct gaaaggtgtt cagcagggag attttgtcat gaccctgggg
    14581 tatcccggca ctaccacccg ctatatgacc tccatagaag tggacaggca ggaacagctg
    14641 accaacaaca tagggattga agtgaggacc atccgccaga atatactgct ggaagacatg
    14701 ctggccgatc ccaaagttca actccagtat gcaagcaagt attcaggcag caccaatgcc
    14761 tggaaaaagt ggacggggat gaatgaaact ttcggaaaac ttaatgtaaa agcccgcagg
    14821 gcagcagaag aagccgcctt taccgcctgg gtcaatgcca gcaaaaaacg cattgaaaaa
    14881 tacggcacgg cactgaaaga tattgaagaa gccatccagg ggacctggga caatctttat
    14941 gtcagccgtt atctgagtga agccttgcgc aatattgaac tggcttcttt tgcttcgcgg
    15001 gtcgcgcgcc tggtaagcag gccgggagaa gacggaagtg tggacacaga gggacttaag
    15061 gctcaggcag aacgtttttt catcaactac aatgccccta cggacttgaa ggtagccaaa
    15121 gccatgctta aacattttgc cctgaaggtg gacaaagcgt attatccttc tgtttacggg
    15181 at
//
LOCUS       DAOSOF010000034        15160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3944_length_15160_cov_4.841774, whole genome shotgun sequence.
ACCESSION   DAOSOF010000034 DAOSOF010000000
VERSION     DAOSOF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3944_length_15160_cov_4.841774"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLM68_03275"
     CDS             <1..722
                     /locus_tag="PLM68_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT54420.1"
                     /translation="TGKPVITVVMAGRPLTIEKEVSLSNALLYSFHPGTMGGVALADL
                     IFGKEVPSGRLPVTFPKTSGQVPLYYNHNSSGRPFQGNETMLDQIPLKAGQTSLGNTS
                     FYLDAGAAPLFPFGFGLSYTTFAYTNIRIDQVSYHQNDTMTMSFDLTNTGKTAATEVA
                     QLYVCDKTGSVTRPVKELKKFERIALNPGETRTVIWNLPISELAFWNLQMEKVVEPGQ
                     FALWVSGDSASGEPILFEVKK"
     gene            825..1736
                     /locus_tag="PLM68_03280"
                     /pseudo
     CDS             825..1736
                     /locus_tag="PLM68_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            1746..3065
                     /locus_tag="PLM68_03285"
     CDS             1746..3065
                     /locus_tag="PLM68_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT54421.1"
                     /translation="MGKKRKKRIYERNIYYTVLKYYVGLFYKMSYGKREYHGIEKLPV
                     DGALIFAPNHTNALMDAMAVLDLDNRPKVFVARADIFKKPLLARIFYFLRIMPINRIR
                     DGRSALKDNDQIIKNSAEVLKHNVPFVILPEGTHRPMHSLLPLGKGIFRIALLANEEI
                     AGEKPVYIIPMGLEYGNFFRYRSSLLLQIGDPINVTQYVAAHPDREDPVIMNELKEKL
                     EAALKELILYIPDDAFYDATLELCNMSCTRRIIQLDLNSHSLKDRLTANQHTMRLILE
                     RREQDPETSTRVLNEIVRFSANRKRTKISMASVANPRPGLSLAIRLFLLILLFPYFLA
                     CTVLSAPTLLLTRLICSRMEDQAFHNSVRYLTTLLVWTLTVLVSFFVLFGLVAWEHAL
                     AATLVFIPAPLFFYQYYKWFRTARSDFRWLCAPDLRKDKERFVLWIP"
     gene            3053..3535
                     /locus_tag="PLM68_03290"
     CDS             3053..3535
                     /locus_tag="PLM68_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOT54422.1"
                     /translation="MDTVIQILAVLLGILGLLGCLLPVLPGPPVSFVALLLLFFKSDS
                     HMQWTFLLLWGIITIVVSILDYIVPATFTKWTGGSRAATGFATAGLFLGIFFFPPFGM
                     IVGAFLGALLGEMLYGKKEGGLSSSLKPALGSFMGFLAGVGLKLIASGIMMYYIIRYL
                     "
     gene            complement(3519..4319)
                     /locus_tag="PLM68_03295"
     CDS             complement(3519..4319)
                     /locus_tag="PLM68_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT54423.1"
                     /translation="MIFRVTFLGTGTSTGVPLIGCQCRVCQSTDTRDKRLRSSILIKV
                     FSPAGQELSLLIDAGPDFRQQMLTYGISTLDGILLTHEHRDHIAGLDDVRAFNFLSSK
                     PVPVYGEQRVLKALRKEFDYAFAGYNFSGLPKMSLFPITCEPFRIRDVGIVPVRATHG
                     RLPILGFRIGPLGYLTDASSIEERELEKFSGVDLFVVSTVQRTPHNTHFSLQEAIDVA
                     NRVGSPQSLLTHLSHCLEPHKELAADLPPGMAPAYDGMTVELPFKDNG"
     gene            4425..5462
                     /gene="asnA"
                     /locus_tag="PLM68_03300"
     CDS             4425..5462
                     /gene="asnA"
                     /locus_tag="PLM68_03300"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOT54424.1"
                     /translation="MDTTSIPSSYKSILDIWQTEQAIKFIKETFQTELAGELKLRRIT
                     APLLVESGTGLNDNLSGTELPVSFPLKTLGGKDVEIVQSLAKWKRYALWRHHIVPGMG
                     IYTDMNAIRPDEITDNLHSVYVDQWDWEKVILPEERTEKTLRKCVKSIYYAIKRTQFL
                     LSEHFALLQPFFPHDIFFIHAEELRRKYPGLSPRERENAIAREYGAVFVQGIGGPLGD
                     GTVHDCRSPDYDDWSTETGTGFFGLNGDIIIYHPVLDTAVELSSMGIRVNPQSLRHQL
                     ERANAQARASLFFHKLLLDNVLPQTMGGGIGQSRLCLLLLKKCHIGEVQSSVWPQSTL
                     KKCAQADVFLF"
     gene            5604..6563
                     /locus_tag="PLM68_03305"
     CDS             5604..6563
                     /locus_tag="PLM68_03305"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOT54425.1"
                     /translation="MKIIGTGSSLPSLSVTNEMLSVFLDTSDEWIRSRTGILERRIIS
                     DENLKDLAIEASQKALDDAGLTGSELDYIICSNVVNYYVTPSISCLVAGGIQAACPCV
                     DLNAACTGFLYCLDMAAAYIAAKKAKNILVVCAEEPTRMCSWKDRNTAVLFGDAAAAA
                     VVTEGDAFRASRISAFHRPEVLYYQRPLEDCPFVKYQQRGVPLYMNGKEVFKLAVNAS
                     TQGIENILADTGLTADQVDHYILHQANMRIIETVRTQLRQPPHKFPHNIESRGNTSSA
                     SIPLLLDEMHRNGTLQYGDTVVLCGFGAGFTTGTCLFTWTMNE"
     gene            6576..7511
                     /gene="fabK"
                     /locus_tag="PLM68_03310"
     CDS             6576..7511
                     /gene="fabK"
                     /locus_tag="PLM68_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOT54426.1"
                     /translation="MKTIKSRICELTGITYPIFQGGMSWISEARLAAAVSNAGGLGII
                     SAMNADASYLSSQIKLCRELTDKPFGVNILLLSPYVDDVARVVREERIPVVTTGAGTP
                     GKYMEGWLRAGVRVIPVVASVALAKRLEKMGASAVIAEGAEAGGHVGELNTMALVPQV
                     CDALSIPVVAAGGIADGRGIAAAFMLGAEGVQCGTRFLAALECQIHPVYKQKIIEATD
                     SDTIVTGKKLGHPVRALKTPFTRKFAQLENSQDFTQADADSFGAGALRRAAQEGDAVN
                     GCYMAGQSAGLVKKIQSAQDIILEMMQQATALLKL"
     gene            7525..8751
                     /gene="fabF"
                     /locus_tag="PLM68_03315"
     CDS             7525..8751
                     /gene="fabF"
                     /locus_tag="PLM68_03315"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT54427.1"
                     /translation="MRRTVITGMGIISPVGNDIPSFWNNLVNGVCGIGPIQSFDTTDL
                     SVKVAAEVRDFNPAAFGIDPATARKADLFVLYALAAARQAMKESAPDVAPERLGVYVG
                     SGIGGIHTFINECRKLEEHGPNRVSPLFIPNMIANIAAGNIAIAYNAQGPCLPVVTAC
                     ATSTHAIGEAWLAIAHGYADVIIAGGTEAAITPLAVGGFNNSRALSRSSDPKRASIPF
                     DLNRDGFVMGEGAGIVILETYERALGRGATMYAEVTGYGNTCDAFHYTAPRPDGSSAA
                     RAMSLALSTSGQIPDDLLYINAHGTSTPLNDPIETMAIKLALGETLARKALVSSSKSM
                     TGHMLGAAGAVEIIVAALALKEGLVPPTIGLENPDPQCDLDYIPGKSRNAAITLAMSN
                     SLGFGGHNACVALRKI"
     gene            8762..9187
                     /gene="fabZ"
                     /locus_tag="PLM68_03320"
     CDS             8762..9187
                     /gene="fabZ"
                     /locus_tag="PLM68_03320"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOT54428.1"
                     /translation="MVKEEIMEFLPHREPMLLVDEMEVDENRVVHARYTVTGREYFLQ
                     GHFPGYPVVPGVILCEIMGQCCSLLVKEQLVGRTPLYAGLDKVRFKRQVRPGDTIEVS
                     GYITKQKSLVFFVHAEARVAGEICAQGTLSFVLIQRPSE"
     gene            complement(9169..9792)
                     /locus_tag="PLM68_03325"
     CDS             complement(9169..9792)
                     /locus_tag="PLM68_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54429.1"
                     /translation="MKTMLAILCIGTMFSCARRAENKVFEEKVILALEQQLERYPHST
                     VRDVYKNFFQDKFGPGHLLEDTSAAGRYLRRELASYDPDTLPVKGPYIDSTGWEHRFV
                     RVDLRVIEEGKVSYEVFFEAFVRSVQAAPSVPLGSWTREWNRIVGIMEKQQYPLTTHP
                     GYRADKDSIAANLRRGEFVGHHSREYVASYAPHYRLIAGSELHSLGL"
     gene            9863..10477
                     /locus_tag="PLM68_03330"
     CDS             9863..10477
                     /locus_tag="PLM68_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54430.1"
                     /translation="MIWTKLGILIIGFIYAGLIPFTVKRVVRQIDFDLHRQSLSFLSN
                     KTLYDKKMVRGYTQLLFATAVLHYVFFWLLSKYYNLGAHESYMQYLTYSFFFFTLLAF
                     VHHNIRPYSFRRLSSTLQRLAHNMVAIVVFLTLPILIVLFQAAIIENMPFGGITGLVI
                     IGLTVIITLISIIRQGVNGITEIFFINGISFWTVFVTIYTVLFG"
     gene            complement(10481..11416)
                     /locus_tag="PLM68_03335"
     CDS             complement(10481..11416)
                     /locus_tag="PLM68_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54431.1"
                     /translation="MVVPDVLDRAGFIRLGSQAGNTVLNGTVNGDAGSQVRFYYQPDS
                     PTGSATDNRFRRIRVTYEDGSLEHIVYNRQGYGDVQMTAGGAWWSSFNALRNTNPSGT
                     GNFAFNPWSTFLSTYLTYSGWFFKWGNNGGMHPTVPVPGDVNTQNLELTITSNANPKS
                     NHLTGWANITKNVYNRNVTTNRREPCPEGYMVAASAQYTTLASGSMASGYVYDDAGYE
                     MGALIVNGSTNLFFPMGSCGYGIRNGANNGALEYAVSGTPSTWYWARGADCSTAVAHT
                     EFAFEGTTMGVLAIPNIITAGKTQDSGKFVRCVRQ"
     gene            complement(11624..12322)
                     /locus_tag="PLM68_03340"
     CDS             complement(11624..12322)
                     /locus_tag="PLM68_03340"
                     /inference="COORDINATES: protein motif:HMM:NF020418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimB/Mfa2 family fimbrial subunit"
                     /protein_id="HOT54432.1"
                     /translation="MTLRSIRLVFLLAPFLAGYIFEPLAECDNVSIVFRYQEKGAERQ
                     ISEHIEQVHLLVYGQDKKSVQQHILNFDGQSGIYYSNGLNLTPGRYRLVAVGNAFENT
                     VLDAPGNPESLRLTNPAYLAGGRVTSNDSLYLGQKEIEVSPYECLRDTVDMASIHLNL
                     NILIKGLQRMNTKNGNSPVSISMNGLRPVFSPLKGAIDQQQTYYSGGTYYDTLGLFVS
                     RFNIFSGSDSFVAL"
     gene            complement(12329..13414)
                     /locus_tag="PLM68_03345"
     CDS             complement(12329..13414)
                     /locus_tag="PLM68_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54433.1"
                     /translation="MKQLFFAGLAAILMIASCQKDVIPSENQEDYTQSLYIRLNPDNF
                     APATKAITDARTANGAKFATPLKVYILISDEAGNIKHRIPVGTVADAANPQLDIEALK
                     AGYRLNNIYKMAHKVQIAGNPKAGNFAGDMDATLKSMTLYSDVVDLVNDIQYDNDPTE
                     IVLYGEGNISSTQDGNVTRLKAAVTLNPMVSRFQITLDYDEAVIKEVTFEGVYIRNIY
                     QTSTFRNVGGGLNSQGSLEDDYTIGQPKFVADYYTNMFDYNVSGLDTTKVFAYHFFPL
                     TAPEIVIRYKAKNTNDNIEKLRFVRATGFKDGDGNAFSNWLPGELYNVDIVAGRGPDD
                     PVGKNVTLDITISVGERAAVNLTPVYQ"
     gene            complement(13493..13849)
                     /locus_tag="PLM68_03350"
                     /pseudo
     CDS             complement(13493..13849)
                     /locus_tag="PLM68_03350"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
     gene            13918..14574
                     /locus_tag="PLM68_03355"
     CDS             13918..14574
                     /locus_tag="PLM68_03355"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOT54434.1"
                     /translation="MKKIFLFTLIASVFLFNGCSGIVGNGKAVEEIRPVSPAYRGIVV
                     EKAIRVIISDEVTDIHVRADELFMPYLKTFIDEDNMLVVTYSKGIIGYSSVATEVTVP
                     YHSLIADFRASGASRIDSESEMTLNQASFEASGASHLNFMGSAINCQVKLSSASRFQG
                     FDFTAITLNCELSGASRVEITCEGAMKVKASGASKVLYKGNCVITSIETSGASEVTKK
                     "
     gene            complement(14585..14899)
                     /locus_tag="PLM68_03360"
     CDS             complement(14585..14899)
                     /locus_tag="PLM68_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT54435.1"
                     /translation="MTLKSLAPGEEIGLENHADTDQFLRVESGKGIVVMGDSEDALDF
                     REEVKAHFAIFIPAGKWHNMANTGEEPMKIYSIYSPVQHPFVTIHETYEDGMEADSHH
                     ID"
     gene            complement(14896..15114)
                     /locus_tag="PLM68_03365"
     CDS             complement(14896..15114)
                     /locus_tag="PLM68_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54436.1"
                     /translation="MKTILYLLSITLLLMLVSCNCPSDQVDPAQAEEESMVVFQDYGQ
                     LPFVLDIEAYTLKTISTGGQSGQGPICR"
BASE COUNT         3755 a         3825 c         3665 g         3915 t
ORIGIN      
        1 ctacaggaaa acctgtcatt acagtggtta tggccggcag acccctgaca atagaaaaag
       61 aagtgtccct gagcaatgca ctattgtact cgttccaccc cggtaccatg ggtggagtgg
      121 ccctggcaga cttgattttc gggaaagaag tacccagtgg caggctgccc gtgacctttc
      181 ccaaaacctc gggacaggtt cccctttatt ataaccataa cagttccgga cgccccttcc
      241 agggaaatga aaccatgttg gatcagattc ccctaaaggc cggacagact tccctgggca
      301 atacaagctt ttacctggat gccggtgctg ctcccctttt cccgttcgga ttcggactct
      361 cctacaccac atttgcctat accaacatca ggattgacca ggtgtcatat catcagaacg
      421 ataccatgac gatgtccttt gatctgacta atacgggaaa aaccgctgcc accgaagtgg
      481 cccagctcta tgtgtgcgat aagaccggct cagtcacacg tccggtcaaa gaattgaaaa
      541 aattcgagag gatcgcactg aatccgggag aaacccggac cgtgatctgg aacctgccaa
      601 tcagtgaatt agccttctgg aaccttcaga tggaaaaagt agtggaaccc ggacagtttg
      661 cattgtgggt ctccggggac agtgcttcgg gagaacctat tctctttgag gtaaaaaaat
      721 gaaccagccc tgttttgcag catacatttt tttatatatt tgtatgcatg tatgaacaac
      781 tgttaaacct ggaccccatc cggcttaact tctcagccaa tgggatgagg gtaatcaatt
      841 ttgtattggc gctggtaatg tttggggtgg cccttggcat caagccggct gaattcaaaa
      901 acgttttcaa gaagcccaaa tcagctattt tgggactcac ctgccaattg atcctgctcc
      961 cggccatgac gttcttgctg attgtacttt taaaggacca tatcaccccc atggtcgcca
     1021 tgggcatgat actggtagca gcctgccccg gggggaacat atccaatttc atgtcttctt
     1081 tgtccaaggc caacgttgaa ttgtcagtaa cgctcaccgc aatcaccacc tctaccgcca
     1141 ctttcatgac acctttcaat tttgcctttt atggcgggct gtacacccgg tacctgagca
     1201 gaagtgccgg cacagcgctg cagacactgc acatagattt gtgacaaacc ttcgagacgg
     1261 tctttatcct gcttggaata cccctgatcc tgggcatgtt gttctcgcat tttttcccga
     1321 aaataaccga gaaactgaaa aaacccatgc agattttttc tttgttgttc tttattgcgc
     1381 tggtggccat atctttcagc aacaatttcg atctttttat ccggtacatc ggttatattt
     1441 taatcattgt tttcatacac aacgcgcttg cgctgggtat cggctatggg gtaggatctg
     1501 ctttccgtgt accctacaaa gacaggcgaa cgatcaccat agaaacgggt atccagaatt
     1561 cgggacttgg gcttgtgctg ttgttcaatc caaagatctt ccctcctgag ctggagttgg
     1621 gcggcatgtt gtttgtcacc gcctggtggg gtgtatggca tatcatttcc ggcctgggag
     1681 tttctgtatt attcaactgg agagaagccc gcaggacccg gaaagcagtt gcatgatgat
     1741 ttgttatggg caaaaaacgc aaaaaaagga tctacgagag aaacatttac tataccgtac
     1801 tgaagtatta cgttggcctg ttttacaaga tgtcttacgg caagcgtgaa taccacggaa
     1861 tagaaaagct ccctgtagac ggcgctctta tctttgcccc caatcacacc aatgccctga
     1921 tggatgcgat ggctgtcctg gacctggaca accgtcccaa agtctttgtc gcccgggcag
     1981 acatattcaa gaaacccctg cttgcgcgga ttttctattt tttgcgcatc atgcccataa
     2041 accggatccg ggacggacgc agtgccctca aggataacga ccagataatc aaaaactcgg
     2101 ctgaagtcct gaaacacaac gtgccatttg tcatacttcc ggaaggaacc caccggccca
     2161 tgcacagcct cctcccgctg ggcaagggga ttttccgcat tgccctactg gccaatgaag
     2221 aaatcgcagg tgaaaaaccg gtatacataa tccccatggg cctggaatat gggaacttct
     2281 tccgttaccg ttcctctctg ctgctccaga tcggggaccc gattaatgtt acacaatacg
     2341 ttgcagccca cccggataga gaagatccgg tcatcatgaa cgagcttaag gaaaaactgg
     2401 aagccgccct gaaagagcta atattataca ttccggacga tgcattttac gatgccaccc
     2461 tggaattgtg caacatgtcc tgtacccgcc ggatcataca actggacttg aacagccaca
     2521 gtctgaaaga ccgcctgaca gccaaccagc acaccatgcg gctgatcctg gagcgcaggg
     2581 aacaggatcc tgaaacaagc acccgggtat tgaacgagat tgtccgtttt tcagctaata
     2641 gaaaaagaac aaagataagc atggcctctg tggccaatcc ccgcccgggt ctgtcactgg
     2701 ccatacggct gtttcttctc attcttctct ttccatactt cctggcctgt acggtgctat
     2761 ctgcccctac cctgttgtta acccggctga tctgttccag gatggaggac caggccttcc
     2821 ataattcggt acgttacttg acaacgctcc tggtttggac actgactgta ttggtctcct
     2881 ttttcgtatt attcggtctc gtagcctggg aacatgccct ggcggccacc ctggtattca
     2941 tcccggcacc tttatttttc tatcagtatt acaagtggtt ccgtactgcc cggtcagatt
     3001 tcaggtggct gtgtgcaccg gatttgcgca aggataaaga acgttttgtc ctatggatac
     3061 cgtaatccag atcctggccg ttctcctggg tatcctgggt ctcctgggct gcctgcttcc
     3121 cgtacttccc ggccctccag tcagctttgt ggccttgttg cttctgtttt tcaagagtga
     3181 cagccacatg caatggacct tcctgttact gtggggaata atcaccatag tggtcagcat
     3241 cctggactac attgtcccgg ccactttcac caaatggacc gggggcagcc gggctgccac
     3301 gggatttgcc acggcaggct tgttcctggg tatttttttc ttcccgccgt ttggaatgat
     3361 tgtgggggct tttctgggtg ctctgctggg tgaaatgctt tacggcaaga aagagggagg
     3421 gctgtcctca tccctgaaac ctgccctggg atcattcatg ggattcctgg caggggtagg
     3481 gctcaaactt atagccagcg gcatcatgat gtattacatc atccgttatc tttgaaagga
     3541 agctcaacgg tcatcccgtc gtatgcaggt gccatccccg ggggaaggtc agcagctagc
     3601 tccttgtgtg gttccaggca gtgtgaaaga tgagtcagca aggattgggg cgatcccacc
     3661 ctgttggcca catcaatggc ttcctggagc gaaaaatgcg tattgtgggg agttctctgc
     3721 accgtgctga caacaaacaa atccactccg gaaaatttct ccagttcccg ctcttctatg
     3781 ctgctggcat cggtcagata gcccagtggc ccgatcctga agcccagtat gggcaaccgg
     3841 ccgtgtgtcg cccgaaccgg aactatccct acatcccgga tccggaaagg ttcgcacgtt
     3901 attggaaaaa gagacatttt tggcaatccg gaaaaattgt atcccgcaaa ggcgtaatca
     3961 aattctttcc gcagggcctt caaaaccctt tgttctccat aaacaggcac aggtttgctg
     4021 gataagaaat tgaacgcacg cacatcgtcc aacccggcta tgtggtcccg gtgctcatgg
     4081 gtcagtaaaa ttccgtccag ggtggagatc ccgtaggtaa gcatctgctg cctgaaatcc
     4141 ggaccggcat cgatcaataa actgagttcc tgcccagccg gggaaaagac ctttatcagt
     4201 atagaagacc gcagcctttt atccctggta tcggtggatt gacacaccct gcactgacag
     4261 ccaataaggg gaacaccggt agaagttccc gtacccagaa aagtgaccct gaaaatcata
     4321 gaaatgcgat atgccgcttt actttacaca aatgtaataa atataaaatt gtgcagaatg
     4381 aaagtgatgt tgtattttag cattattatg taatacactg gacaatggac acgacctcaa
     4441 tcccctcatc ctacaaatcc atcctggata tctggcagac agagcaggcc ataaaattca
     4501 tcaaggaaac ctttcagact gaattggccg gtgaactgaa actcaggaga attacagccc
     4561 ctttgctggt tgaaagcggc accggcctga acgacaacct gagcgggacc gaattgcccg
     4621 tgagctttcc attgaaaaca ctgggaggaa aggatgttga aattgtccag tctctggcca
     4681 aatggaaacg atatgccctc tggcgacatc atattgtacc gggtatgggg atctacacag
     4741 acatgaatgc catccgtccc gacgagatca cagacaacct ccactctgtg tatgtagacc
     4801 agtgggattg ggaaaaggtg atcctgccgg aagaacgtac agaaaaaacg ctccgtaagt
     4861 gtgtcaaatc catctattat gccatcaaac gcacccagtt ccttttaagt gagcattttg
     4921 ccctattgca gcccttcttc cctcatgata tctttttcat ccatgccgaa gaactccgca
     4981 gaaaatatcc aggccttagc cccagggaaa gggaaaacgc cattgctagg gaatacgggg
     5041 ccgtgttcgt tcaaggaatc ggcggtccgc ttggggatgg gacggtgcac gactgtcgct
     5101 cgccagacta cgacgactgg agcacagaaa ccggaacagg tttctttggc ctgaacggag
     5161 acattattat ctaccatccc gtactggata ctgccgtaga actttcctca atgggaatcc
     5221 gggtaaatcc ccaatccctg cgacatcagc tggaacgtgc caacgcgcag gcccgggctt
     5281 ccttgttctt ccataaatta ttactagata acgttttacc acaaacaatg ggaggtggca
     5341 taggtcagtc ccgcttgtgt ttactgctgc taaaaaaatg tcatattggg gaagtgcaat
     5401 cctccgtctg gccccaatcc acactgaaaa agtgtgccca ggcagatgtg ttcctctttt
     5461 aaagctccgg ggattgcttt ataatgtgtt gtatgtcagt tatttagcat taacgttttc
     5521 cgcctgccgg aatttctttg gaaaccatgg caggattttt gaaccactaa acctttcgtc
     5581 attaaaatgt cgtacaaata actatgaaaa tcattggaac ggggagctca ttaccctcat
     5641 tatctgtaac taatgaaatg ttgtctgttt ttctggatac gagtgacgaa tggattcgtt
     5701 cccgaacggg tatcctggaa cgcaggatca tttctgacga aaacctgaaa gaccttgcca
     5761 tagaagcatc gcaaaaggcg ctggacgatg ccgggcttac aggatctgaa ctggattata
     5821 tcatttgctc caacgttgta aattattatg ttactccctc tatcagctgc ctggtggcag
     5881 gaggtatcca ggctgcgtgt ccctgtgtgg acctgaatgc cgcctgtacc gggttcctat
     5941 actgcctgga catggctgcg gcgtatatcg ccgccaaaaa ggcaaaaaac atcctggtgg
     6001 tttgtgccga ggaacccacc cgcatgtgca gctggaagga cagaaacacc gcagtgcttt
     6061 tcggagacgc ggctgcggcg gccgttgtga ctgaaggaga tgctttcagg gcatcgagga
     6121 tctcggcctt tcaccgcccc gaagtcctct attaccaaag gcccctggaa gattgtcctt
     6181 ttgtgaaata ccagcaacgc ggtgttcccc tgtacatgaa cgggaaagaa gtgttcaagc
     6241 tggctgtcaa tgcttccacc cagggtattg aaaacatcct ggctgataca ggacttacgg
     6301 ctgaccaggt ggatcactat attttacacc aggccaacat gcgtatcatt gaaaccgtca
     6361 ggacgcaatt gcgccagcct ccccataagt tcccgcacaa cattgagtcc cggggcaaca
     6421 cctcttccgc ctccataccg cttttgctgg acgagatgca caggaacgga accctgcaat
     6481 atggcgatac ggtggtttta tgtggttttg gcgcaggatt caccacaggg acctgccttt
     6541 ttacatggac tatgaatgaa tgaatgaata acaacatgaa aacgataaaa agcagaatat
     6601 gcgaactcac aggcatcacg tatcccattt tccagggagg catgtcctgg atttcagaag
     6661 cacggctggc tgctgccgtt tcaaatgccg ggggattggg tatcatttcg gccatgaatg
     6721 ccgatgcctc atacctctca tcacaaatta aactttgccg tgagctgacc gacaagccgt
     6781 ttggcgtgaa catcctgctc ctaagccctt atgtggacga tgttgcccgt gtggtcaggg
     6841 aagaaagaat tcccgttgtc accacaggtg ccggaactcc tggaaaatac atggaaggct
     6901 ggctccgggc cggcgtcagg gtgattccgg tggtggcctc ggtagccctc gccaaaaggc
     6961 tggaaaaaat gggagcctcg gccgttatcg ccgaaggcgc agaagccggg ggacatgtgg
     7021 gcgagctcaa cactatggcg ctggtccccc aggtgtgcga tgcactttcc attccagtgg
     7081 tcgccgccgg tggcatagca gacggacggg gaattgccgc cgccttcatg ctgggtgccg
     7141 agggtgtcca atgcgggacc cgtttcctgg ccgctctgga gtgccagata caccctgttt
     7201 acaagcaaaa gatcattgag gccacggatt cggacaccat cgtaaccggg aaaaagctgg
     7261 gtcatcctgt cagggctctg aagaccccgt ttacgcgtaa atttgcgcaa ctggaaaaca
     7321 gccaggactt cacacaggca gatgcagaca gtttcggagc aggagccttg cgcagggccg
     7381 cacaggaagg agatgcggtc aatggctgtt acatggccgg acagagcgcc ggtctggtca
     7441 aaaagatcca atccgcccag gatatcatcc tggagatgat gcaacaggcc accgccctgt
     7501 taaaacttta aacagtaccc acctatgaga agaaccgtta ttacaggaat gggcatcatt
     7561 tcccctgtgg ggaacgatat tccttcattc tggaacaatc tcgtcaacgg ggtatgcggg
     7621 ataggtccca tccagtcttt tgacaccacg gacctatccg tgaaggtcgc ggcagaagtc
     7681 agggacttca atcctgccgc ctttggaata gaccctgcca cagcccgcaa ggccgacctg
     7741 tttgtcttgt atgccctggc agcagcccgt caggccatga aagagagtgc tccggatgtt
     7801 gccccggaac gtcttggagt atacgtgggt tcgggcatag gaggcatcca cactttcatc
     7861 aatgaatgca ggaagctgga ggaacatggt ccaaacaggg tatccccgct cttcataccc
     7921 aacatgatag ccaacattgc ggcagggaac attgcaattg cttacaatgc acagggcccc
     7981 tgcctgcccg tggttacggc ctgtgccact tctacccatg ccattgggga ggcctggctg
     8041 gccatagccc acggttatgc cgatgttatc attgcaggtg ggaccgaggc ggccatcact
     8101 cccctggccg ttggcggatt caacaacagc cgtgccctgt ctcgctcttc cgatccaaaa
     8161 agggcctcca ttccatttga cctcaaccgg gatggttttg tcatgggaga aggggccggg
     8221 atcgtgatcc tggagaccta cgaaagagcc ctcggccggg gcgcaaccat gtatgccgag
     8281 gtaaccggct acggcaacac ctgtgacgca ttccattata ctgcaccgcg ccctgacgga
     8341 tcgagcgcgg ccagggccat gagcctggct ttatccacct cgggacaaat tcccgatgat
     8401 ttactgtata tcaacgctca tgggacgagc actcccctga atgatcccat agagaccatg
     8461 gccattaaac tggccttggg ggaaacactg gcccgaaagg cgcttgtcag ctcctccaag
     8521 tccatgaccg gccatatgct gggtgccgcc ggagccgttg aaataattgt tgccgccctg
     8581 gccctcaaag agggtcttgt ccctccaacc atcggattgg aaaatcccga tccccagtgc
     8641 gatctggatt atattcccgg aaagtcccgt aatgccgcca tcacacttgc catgtccaat
     8701 tcgctgggat ttggcggaca caatgcctgt gtggctttac ggaaaattta aactcaatgt
     8761 tatggtcaaa gaagaaatca tggaatttct gcctcacagg gagcccatgc ttcttgtgga
     8821 tgagatggaa gttgatgaaa accgggtggt ccatgcccgg tacactgtta ccggcaggga
     8881 atatttcctg caggggcatt tccccggcta tcctgttgtg ccgggggtaa tactctgtga
     8941 gatcatgggc cagtgctgca gcctgcttgt caaggagcag ctggtgggca gaaccccgct
     9001 gtatgcgggt ttggacaagg tccggttcaa acgccaggtc cgccccggtg acaccataga
     9061 agtttcgggg tatatcacca agcaaaaatc cctggtgttc tttgttcatg ccgaggcccg
     9121 tgtggcggga gagatatgtg cccagggaac actttcgttc gttttgattc agaggcccag
     9181 cgagtgaagc tctgagcctg ctatcagacg gtaatgcggg gcataggacg cgacgtattc
     9241 ccggctgtgg tgacccacaa attctcccct gcgcaggttg gctgcaatgg aatccttatc
     9301 ggcccggtaa ccgggatgag tggtgagggg atactgctgt ttttccatga tcccgacaat
     9361 acggttccat tcccgtgtcc atgatcccag aggaacggaa ggagctgcct gcacgctgcg
     9421 cacaaaggct tcgaaaaaaa cttcgtagct gactttgcct tcttcgatca cacgcaggtc
     9481 aacccttaca aaacgatgct cccagcctgt ggaatctata tacggacctt tgaccgggag
     9541 cgtatccgga tcataagagg ccagttcccg cctcaggtat ctgccggcgg ccgacgtgtc
     9601 ttccaacaaa tggccggggc cgaacttgtc ctggaaaaaa ttcttgtata catcccttac
     9661 cgtgctgtga ggatagcgct ccagctgctg ctcaagtgcc aggatcactt tttcttcaaa
     9721 caccttgttt tcggccctgc gggcacagga aaacatggtt ccgatacata gaatggccag
     9781 cattgttttc atggtgtaaa ggtacataag ttgataaaac gggaagattt taccaaattt
     9841 ggaaggttat tagccttttc ttatgatttg gactaaactg ggtatcctga ttatcggttt
     9901 catctatgcg ggactgatcc cctttacagt caagcgggtg gtgagacaga tagactttga
     9961 cctgcaccgg cagtcactga gttttctaag caacaagaca ctctacgaca agaaaatggt
    10021 caggggttat acccaattgt tgtttgccac ggcagtcctt cattatgtgt ttttctggct
    10081 tttgtcaaaa tattacaacc tgggagcaca cgaatcctat atgcaatacc tgacctacag
    10141 cttttttttc ttcaccctcc tggctttcgt gcaccacaac atcagaccct atagtttcag
    10201 acgtcttagc tccaccttgc agcgtttggc ccacaacatg gtggccatag tggttttcct
    10261 gacccttcca atcctgattg tcctgtttca ggcggccata atagagaata tgccatttgg
    10321 gggaattacc ggccttgtta tcattggcct gactgtgatc attaccttaa tctccattat
    10381 ccgtcaggga gtaaacggta taactgaaat attttttata aacggcatca gtttctggac
    10441 cgtttttgtt actatttata cggtattatt cggataaaat ttattgcctt acgcaacgta
    10501 caaatttacc tgaatcctgc gtttttccgg ctgttatgat attgggaatg gccaaaacgc
    10561 ccattgttgt tccttcaaaa gcaaactcgg tgtgcgcaac cgctgtggaa cagtccgccc
    10621 cacgtgccca ataccaggtt gaaggcgttc cggaaactgc atattcaagg gctccgttgt
    10681 tggctccgtt gcgaataccg tacccgcacg aacccatggg aaagaacagg tttgtacttc
    10741 cattgactat caaagctccc atttcatagc ccgcgtcatc atacacatat ccagaggcca
    10801 tgctgccaga agccagcgtg gtatattgag ccgaggcggc caccatataa ccttccgggc
    10861 agggttccct cctgtttgtg gtaacgttcc ggttatagac gttcttggtt atgtttgccc
    10921 atcctgtcag atggtttgat ttgggattag cattagatgt aatggttaat tccaggtttt
    10981 gggtattcac atcaccgggc actggaacag tcggatgcat tccaccatta ttgccccact
    11041 tgaaaaacca cccggaataa gtaagatagg tgctcaagaa cgtactccag ggattaaatg
    11101 caaagtttcc ggtaccgctg ggattggtat tacgcaaagc attaaaggaa gaccaccagg
    11161 cccctccggc ggtcatttgc acgtcgccgt atccctgccg gttatagact atatgttcca
    11221 gcgacccgtc ttcatacgtt accctgatcc gtctgaacct gttgtctgtt gcgcttccgg
    11281 ttggcgaatc cggttggtaa tagaacctaa cctgagaacc ggcatccccg tttacggtgc
    11341 cgttgagcac ggtattgccg gcctggctgc ccaaacggat aaaaccggct ctatccagaa
    11401 catcgggcac taccacgtct acaacatttg atgttcctgt agcgagctat tcagtattta
    11461 cctccctaac cggaagcagg acggccactt tcacggtacg tgtgagcgta cccagtggta
    11521 attttgatcc ggtagttgtc accgtaaggc aaattccaag gattatcaat cctataggta
    11581 tatatggaaa acatgacaat acttctgcca gggagatcgt gcttcagaga gccacgaacg
    11641 agtcactccc gctgaagata ttgaaccggc tgacaaataa tcccagggtg tcataatatg
    11701 ttcctccgga ataataagtc tgctgctgat caattgctcc ttttaagggc gagaatacgg
    11761 gccggagacc gttcatggat atggagactg gcgagttccc gttttttgta ttcatcctct
    11821 gcaagccctt tataagtatg tttaaattca ggtgaatact ggccatgtca actgtatccc
    11881 ttaaacactc ataaggggac acctcaattt ccttttgacc cagatacaaa ctgtcgttgc
    11941 tggttacacg gccaccggca aggtatgcgg gatttgtaag ccgcaggctt tccgggtttc
    12001 cgggagcatc aagaaccgta ttttcaaaag cattgcctac tgccacaaga cgatacctgc
    12061 cgggagtaag gttcagcccg ttgctgtagt atatcccgct ttgtccgtca aaattcagga
    12121 tgtgctgctg aacgcttttt ttatcctgac catagacaag caggtgcacc tgttcaatat
    12181 gttcagaaat ttggcgttca gcacctttct cctggtagcg gaagacaata ctcacgttgt
    12241 cacactctgc cagaggctca aaaatatatc ctgccagaaa aggtgccaat aaaaatacga
    12301 gtcttatgct cctgagcgtc atgatttgtt attggtaaac aggagtcagg tttactgctg
    12361 cccgttcacc aactgaaatg gttatgtcaa gggttacatt cttacccacc gggtcatcag
    12421 gaccacgacc ggcaacaatg tctacattgt ataattcccc cggtagccag ttggaaaatg
    12481 cgtttccgtc tccatcttta aaacctgtcg cacgtacaaa acgcaatttc tctatgttgt
    12541 cattggtgtt cttcgctttg tacctgatca cgatctccgg ggctgtcaaa ggaaagaagt
    12601 gataggcgaa tactttggtg gtatccaatc cggatacatt gtaatcgaac atgtttgtgt
    12661 agtaatccgc tacgaacttc ggctgaccaa tagtgtaatc gtcttcaaga gatccctgcg
    12721 aattaagtcc gcctcctaca tttctgaaag tgctggtttg gtaaatattc ctgatataaa
    12781 ccccctcgaa cgttacttcc ttgatcacag cttcatcata gtccagtgtg atctgaaaac
    12841 gggataccat gggatttaac gtaactgcgg cctttaaccg ggtaacattc ccatcctggg
    12901 tgctactgat gttcccctct ccgtacaaga caatttcagt tggatcgttg tcatattgga
    12961 tatcgttgac caggtccacc acgtcactat acaacgtcat ggatttaaga gttgcatcca
    13021 tatctccggc aaagttgccc gctttgggat tacctgctat ctgtacctta tgtgccattt
    13081 tatagatatt attgagccgg taacctgctt tcagggcttc tatatccagc tggggattgg
    13141 ccgcatcggc gactgtgcct acaggaattc tgtgcttgat atttccagct tcatctgaaa
    13201 ttaaaatata caccttcaag ggtgtggcaa attttgctcc gttagctgta cgggcatcgg
    13261 ttatggcttt tgttgcggga gcaaaattgt cgggattcag cctgatatac aggctctgtg
    13321 tgtaatcctc ctgattttcg gagggaatca catctttttg gcaggaggcg atcatcagga
    13381 tcgcggcaag tcctgcaaaa aataattgtt tcataataaa tgggttggtt tttgttgata
    13441 atttgctttt tgttgtttga ttattactga ttttaagaaa tgcccccccg tagaggaaac
    13501 cctgattaca aacaccctca ataccaagaa aaggaatgtt ccccacattg aattgagata
    13561 ccaggccgtg aaaaccgaaa aaatgaccga aaaaaacagc ccttgaccaa tacctgatct
    13621 cggactgcat catccaatgt ttgatttttc tcaactgtga aaaattccag ggattataat
    13681 tgtcggaaag ttccagggac acctgcttgg atcacgccac ttccatcccc aggttgaagg
    13741 tggttgtggc gtcgtatagt acattggatt tcacggcgac gccttgagcg ttcaagaagt
    13801 gtgagtggaa aagaatcaca ctaatcaggg caatatattt atgccgcata aaaatattat
    13861 taatacgtat tttgatgata ttatgcaaca tctctaaaaa atcgtcatct ttatagtatg
    13921 aaaaaaatat ttctttttac attgattgcc agtgtgttcc tgttcaatgg ctgctcgggt
    13981 atcgttggca atggaaaggc ggtggaagaa atccgtccgg tttctccggc ataccgtgga
    14041 attgttgttg aaaaagccat ccgggtcatt atctcggatg aggtgacaga cattcatgtc
    14101 agggcagacg agttgttcat gccctacctg aaaaccttca ttgatgagga caacatgctg
    14161 gtcgtaacgt attccaaagg aataatcggc tattcttcag tggcaaccga ggtaaccgtc
    14221 ccttatcatt cactgatagc agatttcagg gcttccggtg cttcacggat tgattcggaa
    14281 agcgaaatga cattgaacca ggcttccttt gaagccagcg gggcctctca tttgaatttc
    14341 atgggatctg cgattaattg ccaagtaaag ctaagtagcg cttcgcgttt ccagggattt
    14401 gactttacag ccatcaccct gaactgtgaa ctctcgggtg cgagccgtgt ggaaataacc
    14461 tgcgagggag ccatgaaagt caaggcttcc ggtgcaagca aggtgcttta caaaggcaac
    14521 tgtgtgatca cttccatcga aacctcagga gcttcggaag tgacaaaaaa gtgatcgtcc
    14581 tgtattaatc gatgtgatgg ctgtcagcct ccattccgtc ttcatacgtt tcatgtatgg
    14641 tcacgaaagg atgttgtacg gggctgtata ttgaataaat tttcattggt tcctcaccgg
    14701 tgttggccat attgtgccat ttgccggccg ggatgaatat ggcaaaatga gcctttactt
    14761 cttcacggaa atccagcgca tcttctgaat ctcccattac cactattcct tttcccgact
    14821 caaccctgag aaactgatcg gtgtcagcat ggttttccaa tccaatctct tctccagggg
    14881 ccaggctctt aagggtcatc tgcatatggg tccctgtcca gattgcccgc ctgtagagat
    14941 cgtttttagg gtataggcct caatgtccaa cacaaaaggt agttgaccat aatcctggaa
    15001 aaccaccatg gattcctcct ctgcctgagc aggatccacc tggtctgaag ggcaattaca
    15061 ggatacaagc atgagtaaca gtgtgattga cagtaaataa agaatagttt tcatacgtaa
    15121 atttttaata gtagcattac tgctatataa ttctttttca
//
LOCUS       DAOSOF010000035        15146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_3952_length_15146_cov_5.374727, whole genome shotgun sequence.
ACCESSION   DAOSOF010000035 DAOSOF010000000
VERSION     DAOSOF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15146
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3952_length_15146_cov_5.374727"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1082)
                     /gene="cas6"
                     /locus_tag="PLM68_03370"
     CDS             complement(273..1082)
                     /gene="cas6"
                     /locus_tag="PLM68_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOT54437.1"
                     /translation="MRFKITFNINGDKKLLPFNYQYYISAWIYNVIGSADKTFADFLH
                     NEGFREGNKKFKLFNFSPLDFGKPKIWKEKDLFEIFRDQITLKLSFLLNDVAEAFIIG
                     LFKYKEVYFGDSFNGLDLRVTEIERLSDKVLEETMRYRALSPIVISYQYPGSSFANYL
                     SPENEEYPKLLYNHLRNKYQTVPNTTPLPDFFPFRFTLIGIPRSKLITIKQDTLEQSK
                     IRGYLFDFELTALPEIHHLILSCGAGEKNSLGFGWVEVYNQTDISARPVSV"
     gene            1431..1832
                     /locus_tag="PLM68_03375"
     CDS             1431..1832
                     /locus_tag="PLM68_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54438.1"
                     /translation="MKRKVLLIIASILMIVIGVLRGFGGMALLLKGNNLETNSPIIAG
                     ELQIKLVSSGLLLVCILLIYSAINLLRKYSRKSWNLCFIAMLLFLLGGVLNGFLLFGQ
                     PIDQGQIINIVAVLIISIFLFTGKPALTHMP"
     gene            complement(1920..2924)
                     /gene="deoC"
                     /locus_tag="PLM68_03380"
     CDS             complement(1920..2924)
                     /gene="deoC"
                     /locus_tag="PLM68_03380"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT54439.1"
                     /translation="MDQILSSCGLLCNECEIYPTQCAGCHRVKGAPFWATEHTPGGIC
                     PLYKCAVVDKKYTSCGQCPDLPCELFLRMQDPNSTNEEHQRSLKERVARLKGHQKVYD
                     LAKMIDHSILHPTMTDADLQRECEVALKYHVASVCVKPYAVKTAAALLKNTDVLVGCV
                     IGFPAGNSAIEVKVYEAVKACKDGAVEVDMVINIGKALQGDWNYVENEVKAVTDACHE
                     NGAIVKVIFETDYVNRKEDIIQLCKIATRAGADYVKTSTGFGFVKGDDGKYSYTGATI
                     PNLELMRKSVGPNVRIKAAGGVRTLDALLAVQKAGCFRCGATATAAIMEEAFIRFGSQ
                     "
     gene            complement(2947..3513)
                     /locus_tag="PLM68_03385"
     CDS             complement(2947..3513)
                     /locus_tag="PLM68_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54440.1"
                     /translation="MKNISRKDFIRKACATGACLCGAGCMGFANDIFGVERQDTAATP
                     DKEAMPRQWLFLLLGHIDGIADETQKRSLLKGCAQAHYDHLKLTELLAPYRNNPDAFV
                     QYLESGWGWKVSHDKTTRTILADENKDHCVCPVAARLPQKGPAALCYCSEGFAEAMFS
                     FVLGCPVSATVVSSILRGNERCVYRIVY"
     gene            3798..4694
                     /locus_tag="PLM68_03390"
     CDS             3798..4694
                     /locus_tag="PLM68_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54441.1"
                     /translation="MHLESNDTRKKQVVQFADEIFKAYCVTNFDIDRDGEISLQEALK
                     IRVVYCDNMSIRSLQGIEHFTNLTDLSCSDNKISTLDLSQNRSLEEVYCDLNRLTRLI
                     PGDSPRLQILDCGSNRLKTLDVSRNKGLRYLDCSCNKLSGLDVSENTFLKDLNCGVNL
                     IKTLDVSNNTELEFFSFTVNDIFNIDVSRNEQLRGLDCSMNHLTRLDVRANTRLEFLC
                     CTNNFIKELDVSQNRFLSRLYCYGNSLRTLDLKNNPYLESLHCGANEWDYIDLRNNRS
                     LEELTILREQIDLLEGEIIASA"
     gene            complement(4706..4936)
                     /locus_tag="PLM68_03395"
     CDS             complement(4706..4936)
                     /locus_tag="PLM68_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54442.1"
                     /translation="MATKKKVTRVGGETVTPGPATEPKPFVPTPESKSKATRLRVIAE
                     KDKVLPEAEESGIEGLEDVGEEVKEKVETLVE"
     gene            complement(4941..6158)
                     /locus_tag="PLM68_03400"
     CDS             complement(4941..6158)
                     /locus_tag="PLM68_03400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT54443.1"
                     /translation="MSKEIDVLIIGGGPAGAMTGIELRKKGYSTCIIDKETFPRPKLC
                     AGLLTQKSIDLLAGHCPGLDPSDFVVEKAQTLDFYFQDKKVNNFTTRVNFYLTERTIL
                     DNLLIQYYLKLGGKLLQQVALKPGYIDFRKSTITAGSDKITYRYLAGADGCNGIVSRA
                     AKIRRHDILCMEGNIPRDLLKEKEFRIYFGLTRIGYGWYFPKKDHYCVGMGGEGSGQA
                     ISKIARRFFHEVQNRQAEQAGAAMDKERRTDGCEGLSRETGGAGDSGLKGAFVPSGRR
                     LNTGKLPKNTLLVGDAAGFTEPVTWEGIYYAMQSGVLAAGAIDASAKRNKRNVTRRYN
                     KSVRPIRQNIRAARFWKRILFYPPILKAFMKHLETHTNFALFYLEKVIATGEISYRNF
                     LWVYFTRVRKQGT"
     gene            complement(6325..7629)
                     /locus_tag="PLM68_03405"
     CDS             complement(6325..7629)
                     /locus_tag="PLM68_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT54444.1"
                     /translation="MKRMPFVLLVILSLNVLNSCQKPDSIDPFKHFTMERNQIVVISD
                     IHLGADLAYAECKKNLPYLEEFLNRVRFSSNVKELVIAGDLLDEWFVPANIDTYQGKD
                     QADFVRRIAEANKGVIDVLKKIIREKRILVTYVPGNHDLTISEANVELILPGINQKRD
                     PELGLGTYSPQGYPYIAIEHGHRYNFFCAPDPISNQDIAPGTITPPGYFFTRIAALFV
                     AQGHPDKAADTLPEVTRNATGDESQNLLFAYWNMWAWTLKNFRILNKYDEQLIMTNVD
                     GFTGTFAVNDLIPYQETPGGFIDLDLYNGIQDNWLQRQTRNRVQVPIPALQAISGSAL
                     STETDAQAATQYFLNPRSTVRIVVFGHSHDARIQSSFDHLQQKCIYVNSGTWIDNNPD
                     KTTMNFVIITPQEEDCCSKTHVSLYNFQNEVYTLMAEDSVRL"
     gene            7744..9099
                     /locus_tag="PLM68_03410"
     CDS             7744..9099
                     /locus_tag="PLM68_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HOT54445.1"
                     /translation="MKKIALISANNYQNPYIIYPIGISYIATYLNEKMPDWQLGFFDF
                     NLGGYEDLAAFCRNGNFDYIGVSLRNVDDTNFYEQYSFVSHYRRVMQVIRQNSRAVVI
                     MGGSGFSIYPDVIFKELAPDYGVQGEGEESMRQLVSALEEKKDPTGIDGLVYARPDGT
                     VAINPHKQYISTLALNVEPDKAHFYFEKSGMLNIQTKRGCPYGCIYCSYPIIEGKSVR
                     LLDARSVAENIKELKNSKGIDYLFFTDSVFNIHKEYNRELCSLLIESKVNVKWGAYFT
                     PHNMTYQDMELYKRSGLTHIEWGTDSLSDIQLENYNKKFRFSDVKTQSLNAGKLGIYH
                     AHFMILAGYGETDETLNQTYERSRELGKTIFFPFVGMRIYPRTKLYDIALREGVISAD
                     NNLLIPEYYVSKNVNMETLQDRAHASGQKWFFQGEESPELMERFRAKKKRGPLWEYLM
                     Y"
     gene            complement(9200..10828)
                     /locus_tag="PLM68_03415"
     CDS             complement(9200..10828)
                     /locus_tag="PLM68_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT54446.1"
                     /translation="MKSAIQFLLYTSKGFKSRIGMILLIGLAEVAFSLAFVWISKAII
                     DVATGVRAGELLYYSLVLVGLVLLQILFRVLDNRLRAMTQVRLGNSVRYNVFSRLLYT
                     RWQELSTLHSGDMLTRIIKDTDDVIEVIVNAFPAAILAVVQLLGALAILFFLDPMLAL
                     ILGVGMPLLAIFSRFYYRYMRKYTLEVKESESLITSMMEESLLNQVVIRTFERQENEL
                     DRLGKLQSRLHRSVHKKTRVSVLANLFMGVTFNGGYITAFLWSAFGLAKNTISFGTVT
                     AYLQLVSRLQRPLFELARLLPTLVGSKAAIERLIYLTGFSLEESKDKILLQGNVTLRI
                     ENLTFSYAPDTDPVLSNFSMVLEPGTMVALMGETGVGKTTLLRLLLALVAPDSGRIVL
                     EDESGQVAVSERTRSNFVYVPQGGSLFSGTIRENLLLGDPGANEQELIRALEIASANF
                     VLQLSQGLDTVLGENGKGLSEGQAQRVAIARALLRPGKILLLDEATSALDSQTARTFL
                     QNLKKEAGNRAILFITHQAEVAAFCDRRYDLSPA"
     gene            complement(10913..12025)
                     /locus_tag="PLM68_03420"
     CDS             complement(10913..12025)
                     /locus_tag="PLM68_03420"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT54447.1"
                     /translation="MDDSAINVLFLKLLSAAIWDKPADVTLFNNLDDRTWKQIFRLAR
                     LQSVSALIACKALSLPQECLPPRQQNAALVSHVEQTRARNLKMMGVLATITDWYEEAG
                     FPFVLLKGLANGINYPEPLLRNPGDVDLWLYRKGDSERAKTWLESKGYETIDGNGIHY
                     IFTEQGIHIENHRRVSYFDHKKCDRQLAAMEKQAAEDEHFPFIQLGDLKVRQLPVEMN
                     AFFLFHHMFRHFEHLGVGFRQFCDWILFLKNNRGQIDPVSFTVQAQSYKLLYPMQVFA
                     CAAVKYLDASPDIFPFTVPTDNAFAHKVMKDVLEGGNFGFHHPGKKRPEQKMRGMWFS
                     YKTTVRKSLKFWSLSPQHILILPATKLIHRLRIGFK"
     gene            complement(12230..12409)
                     /locus_tag="PLM68_03425"
     CDS             complement(12230..12409)
                     /locus_tag="PLM68_03425"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOT54448.1"
                     /translation="MKLRDHISRVITEKYEKVAELSKVKDLSVEQGRAYVDAYVDYTH
                     TLEAIEAVIAHGEHH"
     gene            complement(12533..13393)
                     /locus_tag="PLM68_03430"
     CDS             complement(12533..13393)
                     /locus_tag="PLM68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT54449.1"
                     /translation="MKSIPYIEYTKLQARDKNAGQWYYMSHLANPCASADYHRTSAYA
                     ICLLNEGALRLESDLFVNNAQAPAIFTIAPSAIRKFSDLGIDYEARILFFRKEVFLEG
                     QTDINYLDKFDFFEKTGQQVVTLNAAQFQQFRSYFDLIHEKANETAPHTTAIIRSLIN
                     IILNEIEDVHQSQQAGQPVTTDKGDHILSRFKTLLSSHFIQERQVSFYAGKMHLTPKY
                     FSSLIKQDSGKTAGEWISEMLLLESKVRLQDKSQSIAQIAYDLNFSDPSHFGKFFKKH
                     TGMSPLKYRS"
     gene            complement(13344..14567)
                     /locus_tag="PLM68_03435"
     CDS             complement(13344..14567)
                     /locus_tag="PLM68_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54450.1"
                     /translation="MKNKRIFGLQRNVFFTGLTSFFTDTSTKMIYSVMPLFLLSIGAS
                     KTSISLIEGIAESTASLIKAFSGYWSDKIGKNKPFMIFGYGITALITPLYALVRLPVQ
                     VLLLRFVERTGKGLRAAPRDSLISASVSKEETGKNFGFHKAMDNSGAIIGPLVAFLLL
                     SLFPLNYSNIFLIATIPAILGVITIAIFIKEAKTAKNENQFKFHFNQLPKKFYFFLFI
                     VFIFTLGNSADALLLVKTAETGINKSYVPFVYMIFNTVSVILSVPLGKISDKIGRELF
                     IILGFLIYSIVYFFFGRFNSLNVFLFLFVLYGLYSALVDGTQKSLISDIVSEDVKGTG
                     FGLYHAVLGITLLPASLIAGLLYDHVNSNAPFYFGSLMALIASLLMVIFVIVDRKNKS
                     EIHTVHRIYKAPGKR"
     gene            complement(14586..15146)
                     /locus_tag="PLM68_03440"
     CDS             complement(14586..15146)
                     /locus_tag="PLM68_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOT54451.1"
                     /translation="MNEEMLFESRDEFREWLGKNHASSNGFWMVFGKDGLLKTLKPDE
                     ALEEALCFGWIDGQIKSIDQAKYVKKFTPRRKGSVWSEKNKKLVKALTENGKMTASGI
                     AAVELAKKGGQWNTREALSISEEQVGILISALSGASLALSNFMKMPDSVKRTYTALYL
                     DAKKEGTRVKRLEKIIGRLNENKKPM"
BASE COUNT         3820 a         3649 c         3523 g         4154 t
ORIGIN      
        1 catgctcatt gttccatccg agccatttat gaattgtccc agtcaaaaaa ggctgatgat
       61 caaatggtac aattttgtta gttgtcttaa ttttcaaatg gattctcata agctacattc
      121 atttttagaa attaatgatt ttatcaacat cggctaacgc atcgccggtt agcctgatca
      181 gccattcata atcaattgtt ctgtggcttt atttcattta attcgtattt gttctgtttt
      241 ttcattctgt tccggtttcg gaattgtact ttttaaacgc tgacaggtct tgcagaaatg
      301 tcagtttgat tgtatacttc tacccacccg aatcccaatg agtttttctc gccggcgccg
      361 caagagagta ttaagtgatg tatttctgga agagcagtaa gttcaaaatc aaagaggtaa
      421 cccctgatct tgctttgttc cagagtgtct tgttttatgg ttatcagttt agagcgggga
      481 attccgatca acgtaaaacg aaaggggaaa aaatcgggca gcggtgtggt attgggtaca
      541 gtctggtatt tattccgtaa atggttgtaa agtaatttgg gatattcttc attttccggt
      601 gataaatagt tggcaaaaga cgaaccggga tactgataac ttataacgat cggcgaaagg
      661 gcccggtacc gcattgtttc ttctagaacc ttatctgaaa gccgttcaat ttctgtcacc
      721 ctcagatcaa gtccgttaaa agaatctcca aaatatactt ccttatattt aaaaaggcca
      781 atgataaaag cctctgctac atcattcaac agaaaagata gttttagtgt tatttgatcg
      841 cgaaaaattt caaaaaggtc cttttccttc catatttttg gctttccaaa atcaagcgga
      901 gaaaaattaa acagcttgaa ttttttgttt ccttcacgaa atccctcatt atgtaggaaa
      961 tcggcaaaag ttttgtcagc agaacctatt acgttgtata tccaggcact tatatagtac
     1021 tgataattaa aaggaagcaa cttcttgtcc ccatttatgt taaaagtaat tttaaatctc
     1081 atgcctctga atattataga tcgcctagta aagtcgttca aaaatatctg tttactccgt
     1141 caaaacacga cgtagtaaaa aatattacaa aatatttatt agctgtgaac tgcctgtaaa
     1201 tataacattt gcagtccgga taaacaagac gtgtcgaaaa cactgacacc aaaagccgtc
     1261 atggatcaga cttactctgt caacagattt gcagcaaagc aagagagcct gcccgtgcag
     1321 atctctgtac acactggagt acgtgcaagg taataatttt cggacgttat attttattgc
     1381 cgggtaagta ttaatttgta gtttagcgtg atagataaat atttataact atgaaaagaa
     1441 aggttttatt gatcattgcc tcaattctta tgattgtaat aggagtattg cgaggttttg
     1501 gaggaatggc tttattatta aagggtaaca atttagaaac caatagtcct attattgcag
     1561 gtgaacttca aataaaactt gtgtcttccg ggttattgct tgtttgtatt ttactcatct
     1621 attcagcaat aaatctacta aggaaatatt ccagaaagag ttggaatctt tgctttattg
     1681 ccatgcttct atttttattg ggtggcgtat tgaatggttt cctgttattc ggacagccaa
     1741 tagatcaggg acaaattatt aatattgttg cagtcctgat tatcagtata tttcttttta
     1801 caggtaaacc ggccctaaca cacatgccat gaccaatttc tcactgcaat tgcattagca
     1861 gttctgatct tattcccggc aaccctggaa gcacagaaaa aatgcccggc ttgtttggat
     1921 tactgtgacc cgaaccttat gaacgcttct tccatgatgg ctgctgttgc ggtagccccg
     1981 caacggaagc atcctgcttt ctgaacggca agtaatgcat ccagagtcct gacaccgcct
     2041 gcagctttga tacgaacgtt aggtccaaca cttttacgca tcaattcaag attcggaatg
     2101 gtagcccccg tataggaata tttgccgtca tcgcctttta cgaacccaaa gcctgttgaa
     2161 gttttaacat aatctgcccc ggcccgggta gcgattttac ataattggat gatatcctct
     2221 ttcctgttta cgtaatctgt ctcgaatatc actttgacaa tggctccatt ctcatgacag
     2281 gcatcggtca ccgctttcac ttcattctct acataattcc aatctccctg taacgcttta
     2341 ccaatattga tcaccatatc aacttcaaca gcaccgtcct tgcaagcttt tactgcttca
     2401 taaaccttta cctctattgc agaattcccc gcaggaaaac caatcacaca gccaaccagc
     2461 acatcggtat tttttaacaa tgctgcggct gttttaacgg cgtagggttt aacacaaacc
     2521 gatgcaacat gatattttag ggcgacttca cactctcttt gcagatcagc atctgtcata
     2581 gtaggatgca gtatggagtg gtcgatcatt tttgccagat catatacctt ctgatgtccc
     2641 ttaagcctgg ccaccctttc cttgagtgag cgctggtgtt cctcgttggt actgttgggg
     2701 tcctgcatgc gaaggaacaa ttcgcagggc aggtcggggc actggccgca gctggtgtac
     2761 tttttgtcca ccacggcgca tttgtacaag gggcatatgc ctcccggagt gtgttcggtg
     2821 gcccagaaag gagccccttt cacccggtgg cagccggcgc attgcgtggg gtagatttcg
     2881 cattcgttac acagcaggcc gcaggaagat agaatttgat ccatgattat cgtgtttagg
     2941 taaaattcag tatactatcc ggtacacgca cctttcgttg ccccggagga tggatgaaac
     3001 caccgtggcc gagacagggc accccaggac aaaggaaaac atggcctcgg caaatccctc
     3061 ggaacaatag caaagcgcag ccggtccctt ctgaggaagg cgggccgcaa cgggacagac
     3121 gcagtggtct ttgttctcat cggccagaat ggtgcgggtg gtcttgtcgt gcgacacctt
     3181 ccacccccac ccgctctcca ggtattgcac aaaggcatcg gggttgttgc ggtaaggggc
     3241 aagcagttct gtaagcttca ggtgatcgta gtgggcctgt gcgcagccct tgagcaggct
     3301 tcttttctgg gtctcatcgg caataccgtc aatatggccg aggagcagga aaagccattg
     3361 ccggggcatg gcctctttgt cgggagtggc ggcggtatct tgtctctcaa ctccaaaaat
     3421 atcgttggca aagcccatgc agccggctcc gcacaaacag gctcccgtgg cacaggcctt
     3481 ccggatgaaa tcctttctgc tgatgttttt catggtactt tacaggctgg tggaatgtaa
     3541 agttaaattt atttagacct ttgacaataa aagcaatcta aaatgatgaa acgaatgcga
     3601 cattctatca tgccaacggc cagaacctgg tcaaggcatg ggaatactgc ttacctgatc
     3661 tttggatctg ccttcaagga catggtgaaa aagtgaccgc gttgcactct aaatgcatga
     3721 tgataaagat tgcatcctgt tacctttttt tgtaaattta cactaccagc tccctaattt
     3781 ttaacttttt atttaccatg catttagaat caaacgacac ccggaaaaag caggttgttc
     3841 aatttgccga tgaaattttc aaagcctact gcgtcacgaa tttcgacata gacagggacg
     3901 gagagatttc cctgcaggaa gccttgaaaa tccgggtggt ttactgtgac aatatgagca
     3961 taaggtccct gcagggaatt gaacatttta ccaatctgac agacctgtct tgcagtgata
     4021 acaagattag caccctggat ttgagtcaaa ataggtcatt ggaagaggtc tattgtgacc
     4081 tgaaccggct cacccggctt atcccgggag acagtccccg cttacaaata ctggattgcg
     4141 ggtccaacag gttgaaaacc ctggatgtga gccgtaacaa aggattgcga tacctggatt
     4201 gcagctgtaa caagctgtcc ggactggatg tgagtgaaaa taccttcctg aaagatctca
     4261 attgcggggt gaaccttatc aaaaccctgg atgtaagtaa taacacggaa ctggaattct
     4321 tctcattcac cgtgaatgat attttcaata tcgatgtgtc ccggaacgaa cagcttcggg
     4381 gtctggattg ctccatgaat cacctgaccc ggttggatgt acgtgccaat acaagactgg
     4441 agttcctttg ctgcaccaat aatttcataa aggaacttga tgtgagccaa aatcgctttt
     4501 tatccagact gtactgttac ggcaacagtc ttaggacgct tgacctgaaa aacaacccat
     4561 acttggagtc actgcactgc ggagccaatg agtgggatta tattgacctg agaaacaacc
     4621 gctccctgga ggaactcact atcctccgtg agcagataga cctgttggaa ggcgaaatta
     4681 tcgcctcggc ctgacccggc ttattttatt caaccagtgt ttccactttt tcctttactt
     4741 cctcccctac atcctcgagt ccctctattc ccgactcctc agcttccggc agcaccttgt
     4801 ctttctcggc aatgacgcgc agcctggtgg ctttggactt gctttcggga gtcggcacga
     4861 atggttttgg ttccgtggcg ggtccgggag ttacagtttc cccacccacc ctggtgactt
     4921 ttttctttgt tgccatacta ttacgtacct tgcttgcgta cccgggtgaa gtacacccac
     4981 aaaaagttcc ggtaactgat ttccccggtc gcaatgactt tctccagata gaagagcgcg
     5041 aaattcgtgt gcgtttccag gtgtttcatg aacgccttca ggataggcgg atagaagagg
     5101 atccttttcc agaaacgggc cgccctgatg ttttggcgga tgggccgcac gcttttgttg
     5161 tagcgtctgg tcacgttcct tttatttctt tttgccgagg catctatggc accggccgcc
     5221 agaaccccgg actgcatggc ataataaatt ccctcccagg tcaccggttc tgtaaatcca
     5281 gctgcatcgc ccaccagcag tgtgtttttg ggaagttttc ctgtattcag tctacggccc
     5341 gagggaacga acgccccttt caacccggaa tctccagccc ctccggtttc ccggcttaat
     5401 ccctcacagc catcagttcg cctttccttg tccattgcag ctccagcctg ttcagcttgc
     5461 cggttttgca cttcatggaa aaaacgcctg gcaatcttgc ttatcgcttg cccggaccct
     5521 tcaccgccca tgcccacgca ataatggtct tttttaggga aataccaacc gtacccgatt
     5581 ctagttaaac cgaagtatat cctgaattct ttttctttca gtaaatctct tggaatattc
     5641 ccttccatgc ataatatatc gtgtctcctg atcttagcag ccctgctcac aatcccgtta
     5701 cagccgtcag cacccgccag gtaccggtag gtgatcttgt ccgagcctgc tgtaatagtg
     5761 ctcttccgaa agtcaatata cccgggcttg agcgccactt gctgcaggag cttgccaccc
     5821 agtttcagat aatattggat gagcaggtta tcaagaattg tgcgttcggt taaataaaag
     5881 ttcacccggg tggtgaagtt gttcaccttt ttgtcctgaa aatagaaatc cagggtttgc
     5941 gccttctcta ccacaaagtc cgatgggtcc agcccaggac agtgcccggc aagcaggtct
     6001 atggattttt gtgtcaaaag ccctgcacac agcttaggac gcggaaaggt ttccttgtca
     6061 attatacagg tgctgtaacc tttcttcctc aactcaatgc ccgtcatggc tcctgccgga
     6121 cccccgccaa ttatcagtac atcaatctct ttactcatca gatgactcta ttttgtatta
     6181 cccttaaata tacgaaaaaa aataagtgcg ttgcacactt gattcgtttg cctcataatc
     6241 aatcacttac gaaattcata cccagggggt atgaatttcg taatactctg tatatcagca
     6301 taaaggacaa tgtatgcgac gcactcaaag ccgcaccgaa tcctctgcca tcagcgtgta
     6361 gacttcattc tggaagttgt agaggcttac gtgggttttg gagcaacaat cttcctcctg
     6421 cggtgtgatg atgacaaaat tcatggttgt tttgtccggg ttgttgtcaa tccaggttcc
     6481 ggaattgaca tagatgcatt tttgctgcaa atggtcaaaa gaggactgga tcctggcatc
     6541 gtgcgaatgg ccgaacacaa cgatcctaac ggttgatctg ggattcagga agtactgggt
     6601 tgcagcctgt gcatcggttt cggtgctaag ggcagatcct gaaatggcct gcaatgcggg
     6661 gatggggacc tgcacccggt tgcgcgtctg tctttgcaac cagttgtcct ggataccgtt
     6721 gtaaaggtcc aggtctatga atccaccggg ggtttcctgg taagggatca ggtcgttgac
     6781 tgcaaacgtt ccggtaaagc cgtccacgtt ggtcataatg agctgttcgt catatttgtt
     6841 caggatcctg aaattcttca aggtccaggc ccacatattc caataggcaa agaggaggtt
     6901 ctggctttca tcacccgttg cattgcgggt gacttccgga agggtatccg ctgctttgtc
     6961 cggatgtccc tgagctacga aaagggcggc tatccttgta aaaaagtatc ccggaggcgt
     7021 tatggtgccc ggagcaatgt cctggttgga gatgggatcg ggagcgcaga agaaattgta
     7081 acggtggccg tgctctatgg caatataggg ataaccctgg ggagaatagg tacccagacc
     7141 cagttccgga tcgcgtttct ggttgattcc cggcaggatc agttccacat tggcctcgga
     7201 aatggtcagg tcgtggttgc cgggcacata ggtgaccagg attctctttt cccgtatgat
     7261 ctttttcagg acgtctatga cgcctttgtt ggcctcagcg atgcgccgta caaaatctgc
     7321 ctggtctttg ccctggtagg tatcgatgtt tgcgggtaca aaccattcgt ccagcaggtc
     7381 tccggcaatg accagttctt tcacattgct tgaaaacctt acgcggttaa ggaattcttc
     7441 aaggtacggc aggttttttt tgcattcggc ataggcgagg tctgcaccca ggtggatatc
     7501 gctgatgaca acgatctggt tgcgttccat ggtaaaatgc ttgaacgggt ctattgaatc
     7561 gggcttttga caggagttta aaacattgag tgacagtatc actaacaaca caaatggcat
     7621 tcttttcata gtcttgatat ttggtacaaa tttagccaat atccggttaa cttaatccac
     7681 ccggactttt tttttacctt tgctgcctca atcctgtgac tataaaaagg ccagtacctg
     7741 attatgaaaa aaattgccct catatctgcc aacaattacc agaatccata catcatatac
     7801 cctatcggga tctcctatat tgccacctac ctgaatgaaa aaatgccgga ctggcaactt
     7861 ggcttttttg atttcaacct gggcggttat gaagacctgg ccgcgttttg ccgcaacggg
     7921 aatttcgatt acataggggt atcgctccgg aatgttgacg ataccaattt ctacgaacaa
     7981 tactcttttg tgagccatta ccgccgggtg atgcaggtca tccgccagaa cagccgggcc
     8041 gtggtgatca tgggcggctc gggcttttcc atttaccccg atgtcatatt caaagaactg
     8101 gcccccgatt atggcgtcca gggggaaggc gaagaaagta tgcgccaatt ggtctcggca
     8161 ctggaagaaa aaaaggatcc gacagggatt gacggtttgg tgtacgccag gccggacgga
     8221 acggtggcca tcaatccgca caaacaatac atctctacgc tggcattgaa tgtagagccc
     8281 gacaaggcgc atttttactt tgagaaaagc gggatgctca atatccagac aaaaagaggc
     8341 tgtccttacg ggtgcattta ctgcagctac cccatcattg aaggcaagag tgtccgtttg
     8401 ctggatgccc gttccgtcgc ggagaacata aaagagctta agaacagcaa aggcattgac
     8461 tatcttttct ttaccgattc cgttttcaat atccacaagg aatacaaccg ggaactgtgt
     8521 tcgctcctga tagaaagcaa ggtgaatgtg aaatgggggg cttatttcac gccccataac
     8581 atgacttacc aggacatgga gttgtataag agatccggtc tcacacacat tgaatggggc
     8641 accgactccc tctccgacat acaacttgag aattacaaca agaagttccg attctccgac
     8701 gtcaaaacgc aaagcctgaa tgccggcaag ctgggcatct accacgccca cttcatgatc
     8761 ctggcaggct acggcgagac cgatgagacc ttgaaccaaa cctatgaacg ctcccgcgaa
     8821 ctggggaaaa ccatcttctt cccctttgtg ggcatgcgta tctacccgcg caccaaactg
     8881 tacgatatcg cactccgcga gggggtcatc tctgcggaca acaacctgct cattcccgaa
     8941 tattacgtct ctaaaaacgt gaacatggaa accctgcagg acagggccca tgcctcgggc
     9001 cagaaatggt ttttccaggg agaagaatca cctgaactga tggaacgctt ccgtgccaag
     9061 aaaaagcgcg ggcccctgtg ggagtactta atgtactaga ggtactcaag ctccaacccc
     9121 tattaccagc taccgctact tttcttactg ctgcagctct tgcagacgca ccggattctg
     9181 gctgtaacca ctccgtacct tatgccggtg aaagatcgta ccggcggtcg caaaaggcag
     9241 ctacctcggc ctggtgggta ataaaaagaa tggcgcggtt ccccgcctct ttcttcaaat
     9301 tttgtagaaa agtgcgtgcc gtttgggaat cgagcgccga ggtggcctcg tccagcaaca
     9361 ggattttccc cggacgcagc aacgcgcggg cgatggccac acgttgagcc tgcccttccg
     9421 acaatccctt cccgttttct cccagcacag tgtccaaccc ctgggacagt tgaagcacaa
     9481 aatttgccga ggcgatttcc agcgccctga tgagttcctg ctcattggcc ccgggatctc
     9541 ccaggagcag gttctcgcgg atggtcccgc tgaagagcga acctccctgg ggcacgtaaa
     9601 caaaattgga gcgggtacgt tccgatacag ccacctgccc cgactcatct tccaggacaa
     9661 tgcgccccga atccggtgct accagtgcca gcagcagcct gagcaatgtg gttttaccca
     9721 cgccggtttc tcccatcagg gccaccatgg tccccggttc cagcaccatg gaaaaattgg
     9781 acaaaaccgg gtcggtgtca ggtgcgtagg aaaaggtgag gttttctatc ctcagggtta
     9841 cattcccctg gagaagtatt ttgtctttgc tctcttccaa cgaaaatccg gtcaggtata
     9901 tcagacgttc tatggccgcc ttggagccca ccagggtggg cagcagcctt gccagttcaa
     9961 agagcgggcg ttggagcctg ctgaccagtt gaagataagc cgtcacggta ccgaaggaaa
    10021 ttgtgttttt ggccagcccg aaggcactcc agagaaaagc cgttatgtag ccgccgttga
    10081 aggtgacccc catgaacagg ttggccagga ccgataccct tgttttttta tggacgctcc
    10141 gatgcagcct ggattgcagc ttgcccaacc tgtccagttc attttcctgc cgttcaaaag
    10201 tgcggatcac cacttggttt aaaaggcttt cttccatcat ggaggtgatc aggctctcgc
    10261 tctctttcac ttccagcgtg tatttgcgca tgtacctgta ataaaacctg ctgaaaatgg
    10321 ccagcagcgg catgcccacc cccaggatga gggccagcat cgggtccagg aaaaaaagga
    10381 tggccagcgc tcccagcaat tgtaccactg caaggatcgc tgcgggaaat gcattcacga
    10441 tgacttcaat cacatcgtcc gtgtccttga tgatccgggt gagcatgtcg ccgctgtgca
    10501 gggtggacag ctcctgccac cgggtgtaaa gcaggcggga gaaaacattg taccggaccg
    10561 agttccccag gcgcacctgt gtcatcgcac gcaaccggtt atccaatacc ctgaaaagta
    10621 tctgcaggag taccaggccc accagtacca gggagtaata cagcagctcc cctgccctta
    10681 ctcccgtggc cacatcgatg attgctttgg agatccagac aaacgccaaa gaaaaagcca
    10741 cctcggcaag accaataagc aagatcatcc caatgcggga tttgaatcct ttggaggtgt
    10801 aaagcaggaa ttgaatggcg gacttcattg tgctggcggg ttcaagtatt ggtgctgttt
    10861 tattgcgggc attaagcagt ggctgttggc attaatttta cggccggcag gttcatttga
    10921 acccgattct gaggcggtgg atcagtttgg tggcaggtaa tatcaggatg tgctgggggc
    10981 ttagcgacca gaatttcagg gacttgcgca ccgtcgtctt gtaggaaaac cacatgccgc
    11041 gcatcttctg ctccggccgc tttttcccgg gatggtgaaa gccgaagtta cccccttcca
    11101 ggacatcctt catcaccttg tgggcaaagg cgttatctgt aggcactgta aaggggaaga
    11161 tgtccggcga ggcatccagg tattttacgg cggcacaggc gaatacctgc atgggataca
    11221 gcagtttgta agattgggcc tggacggtga atgaaacggg atcaatctgc ccgcggttat
    11281 tcttcaggaa caggatccag tcgcaaaact gccggaatcc aacgcccagg tgctcaaaat
    11341 gccggaacat gtggtggaaa agaaagaacg cgttcatttc caccggcaat tggcggactt
    11401 tcagatcacc cagctggata aagggaaaat gctcatcctc tgccgcttgc ttctccatgg
    11461 cggccagctg cctgtcacat tttttatggt caaagtaaga tacgcgccta tggttttcta
    11521 tgtggatgcc ctgttcggta aatatgtaat ggataccgtt tccgtctatt gtctcatacc
    11581 ctttgctttc cagccatgtt ttggcccttt cgctgtctcc cttacggtat agccagaggt
    11641 ccacatctcc cggattgcgc aggagcggct ccgggtagtt tatcccgttg gccagtcctt
    11701 taagcagtac aaaagggaag cctgcttctt cataccagtc tgtgatggtg gctaaaacac
    11761 ccatcatctt caggttcctg gccctggtct gttccacatg ggatactagt gccgcgttct
    11821 gttgccgggg aggcaggcac tcctgcggca gggataatgc tttgcatgct atgagcgcac
    11881 tcacgctttg cagcctggcc aagcggaaaa tctgtttcca ggttctatca tccagattgt
    11941 taaatagtgt aacatcggca ggcttatccc agatagcggc actcaacagt ttcagaaaca
    12001 aaacatttat agcagaatca tccatcagtg caaagtttgt caattattgt cttttctgca
    12061 acaagagggg gaacttcttt gcccttatgg caccgcgtac acttgattca tcatgctgga
    12121 tatcagcact ataacaaaag agcccccggg gagagctctt ttgttaatca cctgttaaac
    12181 aaggcaatac atcaaaagta cacggtactc cttcccgagc ggacccttct cagtgatgct
    12241 cgccgtgagc gatcactgcc tcgatggctt caagcgtatg cgtgtaatct acataggcgt
    12301 ccacgtatgc cctgccctgt tcaacagaaa ggtccttgac cttactcaat tccgccacct
    12361 tttcatactt ttccgtgata acccggctga tgtggtccct cagtttcatg atttcacttt
    12421 tttaatagta tgttttcacg tgttcttatc cgggaacaaa agtacaccgt ctttcattgc
    12481 aaaggaaagg atgaaaggag gcaattatgg tataaaagtc ggatttccgg gatcagctcc
    12541 tgtacttaag aggactcatg ccggtgtgct ttttgaaaaa tttcccgaaa tgagacgggt
    12601 ccgaaaaatt caggtcgtag gcaatctgcg ctatactttg cgatttgtcc tgtaacctta
    12661 ctttggattc cagaaggagc atctcactga tccactcacc ggcggttttc ccgctgtctt
    12721 gtttgatcag ggaagaaaag tacttggggg tgaggtgcat ttttccggca tagaaagaga
    12781 cttgtctttc ctgaatgaag tgagaagaca gcaatgtttt gaagcgggaa agaatgtggt
    12841 ctcccttgtc agtggtgacg ggctgtccgg cttgctggct ctggtgaacg tcttctattt
    12901 cgttgagaat gatattgatc aggctccgga taatggctgt ggtgtgcggg gccgtttcgt
    12961 tggccttttc atggatcagg tcaaaatagg accgaaattg ttgaaattga gcggcattga
    13021 gcgttacaac ctgttgtccc gttttctcaa agaaatcgaa tttatccagg tagttgatat
    13081 cggtttgtcc ttccagaaat acttctttcc tgaagaaaag tatccgggct tcgtaatcga
    13141 tcccaaggtc agaaaatttt cttattgcag aaggggcaat ggtaaaaatg gccggagctt
    13201 gggcattgtt gacaaacaga tcgctctcaa gacggagcgc tccctcgttc agcaggcaga
    13261 ttgcataagc cgaggtgcgg tgatagtcgg cagaagcgca gggatttgcc agatgcgaca
    13321 tataatacca ctggccggca tttttatctc ttgcctggag ctttgtatat tctatgtacg
    13381 gtatggattt cacttttatt ttttctgtca actatcacaa atataaccat tagcaatgaa
    13441 gctatcaaag ccattaagga accaaaataa aacggggcat ttgaatttac gtgatcatac
    13501 agcaagcctg caatcaagct ggcgggaagg agtgtaattc caagtacggc atgatacagt
    13561 ccaaatccgg tgcctttcac gtcttcgctt acaatatcgg atattaagga tttttgagtg
    13621 ccatcgacca gggcactgta taatccatac aacacgaaca ggaataaaaa aacgttcagg
    13681 ctattgaacc tgccaaagaa gaaatatacg attgagtaga tcaaaaatcc aagaatgata
    13741 aatagttcac ggccaatttt atctgatatc tttcctaggg gtacggacag gatcacggaa
    13801 acggtattga agatcatata tacaaaagga acatatgatt tattgatacc tgtttcagct
    13861 gttttgacaa gcaataatgc gtctgcggaa ttgccaaggg taaaaatgaa aactatgaac
    13921 aggaagaagt aaaacttctt aggcagctgg ttgaaatgaa atttgaactg attttcattt
    13981 ttcgctgttt ttgcttcctt gataaaaatt gcaatagtga ttacgcccaa tatggcagga
    14041 atggtggcaa taaggaaaat gttggaatag tttagcggga aaagggacaa tagaaggaat
    14101 gctaccaggg gtccgatgat tgccccgctg ttatccattg ctttatgaaa tccgaaattc
    14161 tttcctgttt cctcctttga taccgaggca ctgataaggc tgtccctggg tgcagccctt
    14221 aaacctttgc ctgtcctttc aacaaacctc aataacagga cctggaccgg gagcctgacc
    14281 aaagcataaa gcggagttat cagggcagtt atcccataac caaatatcat gaatggcttg
    14341 tttttaccaa tcttatcact ccagtatccc gagaacgctt tgatcaaaga cgcggtgctt
    14401 tctgcaattc cttcaatcag cgagatcgaa gtcttggatg ctccgataga cagcaggaaa
    14461 agaggcatca cactgtaaat cattttagtg gatgtgtccg tgaaaaaact ggtcaacccg
    14521 gtaaaaaaga catttctttg caggccgaat atcctcttgt ttttcattct ttatgttgtc
    14581 actttttaca tcggtttctt gttttcattg agccttccga tgatcttttc caaccgtttt
    14641 accctggttc cttccttttt tgcatctaaa tacaacgcgg tataggttct ttttaccgag
    14701 tcaggcattt tcatgaagtt ggacagtgcc agactggcac cggacagagc gctgatcagg
    14761 atcccgactt gttcttcgct tatggatagt gcttcccggg tgttccattg tccgcccttt
    14821 tttgccagct caaccgctgc tattccggat gctgtcattt taccgttttc tgtcaaggct
    14881 ttcaccaatt ttttattctt ctctgaccac acgcttccct tgcggcgcgg tgtgaatttc
    14941 ttcacatatt ttgcctggtc gatgctcttt atctgaccgt caatccaccc gaaacacaac
    15001 gcctcttcca gtgcctcgtc cggttttaag gttttaagaa gcccatcctt gccaaaaacc
    15061 atccagaatc cattgctgga agcatggttt ttgccgagcc attccctgaa ctcatcccgg
    15121 ctttcaaaca acatttcctc attcat
//
LOCUS       DAOSOF010000036        14945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4024_length_14945_cov_5.442646, whole genome shotgun sequence.
ACCESSION   DAOSOF010000036 DAOSOF010000000
VERSION     DAOSOF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_14945_cov_5.442646"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLM68_03445"
     CDS             <1..432
                     /locus_tag="PLM68_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54452.1"
                     /translation="VSATAAGSKSTQRLGVPKSSPFQMVSATAAGSKSTQRLGVPKKL
                     SVPNGVSNCIRQQKHEPQRAAKARTRESLGKKKIASRTRGVGELKFLQRSSALGLYWS
                     GPGSKITARSSLAGFLLLVSLKLTPPGAVSPGFLSQVNKND"
     gene            455..1135
                     /locus_tag="PLM68_03450"
     CDS             455..1135
                     /locus_tag="PLM68_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54453.1"
                     /translation="MKRIHNRNILSGIASLIMIIAVFILLSGCIRKSMQKEVERVVEE
                     VLAYSARTIEPELKLLRVKLDRSLTDKEKQLIDQYRDQAKVMIDSMINFQVKLSELEG
                     EKEYRLTEDEISYYNDSQKKMRQIITSCWAVLDNHNEAFALLQRELYPQTVTWQKEVM
                     EIVSTNLPAGFRDEFAKEGSGIKVIGMEQLSPNNMQQVFFILYEPKTNFPLNKVKDML
                     QRMLEDNR"
     gene            1144..2808
                     /locus_tag="PLM68_03455"
     CDS             1144..2808
                     /locus_tag="PLM68_03455"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOT54454.1"
                     /translation="MVDYLKHFFTTQSIPSTLLYLCFAAFTGSMLGKIKFKGIGLGVA
                     GVLFTGILVTHFGAPVDSHTLLFIRDFGLILFVYSIGLSMGPRFFSSFRKDGLLLNLF
                     AVAIVTGGFGIALLICRFTDMSPSAVAGLLSGAVTNTPSLGAAQEVLAEQGAGAAVIA
                     ETGMAYAITYPFGILGVIISMIFIRLLFKINTRSEEEDYTKSLGSSEKTLQNVQIHVI
                     NLDLIGKNIAYIKKMLDNELTISCINRNGEHIAAEDEEIIREGDIICGVSAQDKIENL
                     EFKAGRVTLANIIEDIPGPLGMINILMTNHKIAGKTIEQIGIYRRYPANVTRIYRSGM
                     KILPTLQTTLELGDTLRIVGKKEILNKVKDELGDSINELVKPNIISIFLGIFTGIILG
                     SIPIFIPGLPAPARLGLAGGPLLIAIILGYKGRIGKLSFYMTPGASLIMREMGIILFL
                     SSVGLLSGGKFLETIQHGGWQWMLYGIAITLIPIMVMGIIARLMKVNYLKICGMLAGS
                     MTDPPALEFANGIAPVQAQASAYGIVYPLTMFLRVLLAQVFILMTL"
     gene            complement(2812..3504)
                     /locus_tag="PLM68_03460"
     CDS             complement(2812..3504)
                     /locus_tag="PLM68_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOT54455.1"
                     /translation="MSEIMNIPVIIMDFTRVYRNQSFYLGYDHIIIDCSDIPGSDCYC
                     NQDAAKTIQARIAAHPLRAVHFLDTGNHHYITKFWTDRIDRDFMLVVADHHHDMQPPM
                     FEDHLSCGGWVKTVLETNPFLKQVLFIGVSGEYPLDGNDVRIFRYPAMPVMSLPAYIS
                     IDKDVFHRRVVTTNWDQGDMSCSQVHTILKDIMCNYPVLGVDICGECSTFMDNRSDLV
                     NGRLLSCIAAGF"
     gene            3587..4723
                     /locus_tag="PLM68_03465"
     CDS             3587..4723
                     /locus_tag="PLM68_03465"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOT54456.1"
                     /translation="MVQMRGLEKGKEPLKAVFPGKYIQGRNALSDLPYVLHVMGENGL
                     LLASPSVISNVLPKYSFIESRKNIHIETFGGECCSREVERLSGAVHHTKADVIVGMGG
                     GKVIDTAKIVADKTGIPVIVVPTIASTDAPTSGCAVLYSPEGVFDSVYYLKMNPQAVL
                     VDETIIASAPTRFLVSGMGDALATWFEARSCKRSGSSNECGGYTTLTAMGIARLCYQT
                     LLRYGMQAKKDAELHLTTPALSCIIEANILMSGLGFESCGLASAHAIHNGLTALEETH
                     AFYHGEKVAFGVLAGLYLTGAPVKEKDRVYGFCESVGLPTTFEDLNLEKVSRTRLMKA
                     AVKTCDPKEAIHHETRKVTPEEVLAAMLAADDYGKKRKLGAYGR"
     gene            4713..5303
                     /locus_tag="PLM68_03470"
     CDS             4713..5303
                     /locus_tag="PLM68_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOT54457.1"
                     /translation="MADSPEKQELLKSYFVNYSPRLTNYAVRFVTNRETAQDIVQDCF
                     MHLWEKMEQFSFIHIKALLFTMVRNRCLNYLKHRDVVDLYTGRYLHEVEGEERIYHYD
                     FDFGPDKLPLYEELEKQLQIAVDSLPEKCRKVFVMSRFDGFKNREIAEKLSISTTAVE
                     KHIRKALTTLTSYFEKQYPFHICIILFSCLLSSYLD"
     gene            5399..6400
                     /locus_tag="PLM68_03475"
     CDS             5399..6400
                     /locus_tag="PLM68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOT54458.1"
                     /translation="MKSDQEKIKELAIAYFEGKIHLKEEGLLFDFISQSKENKSLFRK
                     WEHDWLLEHKPDQQTFMLWDRLSAGYSSRKKQLLGSPIVRKNILYRRIFQGVAAAAVL
                     AVVFLGIELGRRSGQDGGYAFVIETQNAEQSNVILPDGTVVYLNAATTLRCPNRYNQK
                     HRVMELEGEAYFEVARNEDMPFIVKAKNIPIKVLGTRFNVTAYADEPTVTTALLEGRL
                     EIGGENKKITMNPDDIIRFDTNTQTVSKFRQNASQYISWTERRFEYNMITFDELFKRL
                     SRQYDVNIIFTADKKMDKIFNISLNNGETITDVLDALSLIIPFTTKRDGKNLYITFK"
     gene            6407..9787
                     /locus_tag="PLM68_03480"
     CDS             6407..9787
                     /locus_tag="PLM68_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54459.1"
                     /translation="MHFNLNLKRLVLTSGLILLGVFSFGQTITREFVNVSLVNVLKEV
                     EKQTGMSIIYETSEVNESYLVTARFENASVEEVLQTVLEGSLYYSIEGKIIVLHKQPS
                     EKDKSIPQVQQQQEHRVTGTVVDESGLSIIGASIVAQGSQTTAITDLDGNYSIRIPAD
                     GVLVVSYIGFVTQTIPVNNQAEINIVMSEDRLVLEEVVVVGYGTQKKKLLTGANLNIK
                     EEDITKRSSQNALDALIGQSPGVQIIASSGQPGEDFRVSVRGLGTVGDATPLYVVDGV
                     QVSGISHINPSEIESIDVLKDAASAAIYGARAANGVLLVTTKKGKTGKTVVGYDGFMG
                     VQSLVRMPEMLNAQDYMMIMDEASINSGGTAYGWLSEFNIDPAKIGQGTDWLNQIIVK
                     NAKTQNHVVSVSGGSQLSVFALSLSYSGQEGVIGGKDYSNNERFNFRINSDHKIYKDI
                     VKIGENLSLSFINRHGVLIGNQFNNIINDALRATPVLTPYNEDGTDYASATLWYVDET
                     NPLASLALRNQKKTQTARLSGDIYLEVQPIKNLKLRSVFALDYSNSNSRSYTPQYYYG
                     SGFYNDTDYVIQQNALRTSWSWENTLNYNFNLKNHNFDILLGIQARQETGQYLSAQKY
                     DLVFNDFDHAWINNATNTNANMVIVEGYPYNLDNLVSYFGRISYNYKEKYMFTATVRA
                     DGSSKFGPTNRFGVFPSASAGWVISSEGFMEGAKDVISFLKLRASWGQNGNNRITDYT
                     YLSTIATTSYAFGDKDNQTSTTTGVYENSMPNVNVKWETSEQLDLGLDLQLFNGKIQA
                     AFDYYNKTTRDWLLQADVLDVFGAQTNPYINGGSVRNKGVEFGASYFGQIGNEFQFSI
                     SANGAFNKNEVISIPNVEGIIHGSQDILFKGMQEMNRAQEGYPIGYFWGLNMLGIFQN
                     QSQIDNHKNSEGKVIQPYAKPGDIIYDDTNDDGDITQADNIYLGQPYPTTTIGLNLSL
                     NYKGFDFYLVGNGSFGMQIAKCYRPIDRKQYNYTADILGRWTGEGTSNTIPRVTNGDE
                     DNGNRLYMSQLYIEDADFFRISNVTLGYNFARLFKQKTVISQLRAYVTVQNALVITGY
                     SGMDPEVGYSGGSTWGSGIDLGYYPRARTVLFGLSIKL"
     gene            9802..11430
                     /locus_tag="PLM68_03485"
     CDS             9802..11430
                     /locus_tag="PLM68_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54460.1"
                     /translation="MKHILKLAAVILVSVNLSSCSDDFLKSEPMTQLTSANFYKTPEE
                     LNMALVGCYQQLRGGYGDFFQLLNIAADDCYGGGGYTDAYGNQIWDEFTHYNDLEINR
                     YTWASKVWRAIRRCNVVVEQAENIDWGADSKLKNQYVAEARMLRAYWYYLTQACYGHI
                     PLVTTSDQLEIEGQADPDDVYAYIAEDLKFAIENLPATRFQDISKGEIGHVTKWFAEG
                     FAARVYLFYTGYYGKSEMPGLTKAQVTGYLNDIITSSGHALLPSFSSLWPWSMRKDYW
                     SKQSLYVGNPNLVYAGEYNPEIVFAIKLHTGFSFSHSQYMGMRGEVKAGDYDPFTSGW
                     GMATITPKAYNWFEEGDTRRFASIVNLAAEGIPCDGTDQREFTGYVHKKYQNLTSPVD
                     RTKGYPQYLGASNKNANPQDQIIMRYSDILLMAAELVVDTNPAQALNYVNQVRNRAFE
                     NTSHAFKSVTKQDILDERAREFVFEGLRYHDVLRQGLTKAKQILDVKGEIVLNGKNPA
                     TKTIDWPIEKEGLLMIPYSDVSLSNGKLLQNPGW"
     gene            11448..13187
                     /locus_tag="PLM68_03490"
     CDS             11448..13187
                     /locus_tag="PLM68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HOT54461.1"
                     /translation="MNKKILVLITLLSFLGINLQAQQPALKKKGDATQMYVDGKPMLM
                     LAGELGNSSSSSEAYLKDVWPGLKALNYNTILAAITWEMIEPEEGVFDFSSVDHLISS
                     ARQYDLKLVLLWFASWKNAASTYIPLWVKKDYQRFPRAEDASGKPVEILSAVSEVNMK
                     ADARAFRSLMKHIAQVDGKEHTVLMMQIENEPGILNTPRDYSPEAELLFNGPIPEDLV
                     QYLKKNKNDLSRDLSKVWSANGSKTKGTWEEVFGKSKIGGWSHTGEFDWHDMYYYTEE
                     LFMGYYYARYMGYVAAEGKKEYDIPMYANAWLKGPDYGWSGMHPGGGPLPSVIDMYHC
                     AGPAIDILSPDIYVPHFTEIVEWYDQLGNPLFIPETRGGELGVSRLLWCLGEHNLMGF
                     SPFGIDRSARTGLTSAQADPLAQAYKLIDGMQEVILEYQGTGNIRGFFVDNDNPEQSF
                     VLGEYIVTADLMRPRNYASMGAPAPTGVRTVPETASGGGFIILQPNGEYIVMARNVNV
                     RFAPAQPGNLPYIGLGTLTEGVFENGEWIQGRVLNGDQIHGSIFTGTGLKMNTLGIQR
                     VTLYRYGKREIKQ"
     gene            13394..>14945
                     /locus_tag="PLM68_03495"
     CDS             13394..>14945
                     /locus_tag="PLM68_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT54462.1"
                     /translation="MKRLLIFYGVLLLCLQANAQPYLQSCAVDVSQDFTDFSNTYFFA
                     DSLASFDPATAEGSIRWKRYSLFGRQAFNTTKVFPLPLEMLDFPPTQYENDPQLRFSI
                     DFVSPVTVRLRVYTSPVILSEEESLMLTGTPPRDGSWTYKKGNGTHTYTGRGGSIVIR
                     EYPFSIMLCDERGREMTRTRHWADNDSTQIKVLPFQFIKRASDNIRSINPVFSLRPNE
                     KIVGCGESPTALNKVGQKVHLFVTDPQSPEGDQMYKPIPFFISNKGYGMFMHTSAPVT
                     CDFGATHAGSAKLFMADETLDLFLFWGKPAEIIDQYTNLTGKATMPPLWSFGTWMSRI
                     TYFSQEEGYEVARKLRENKIPADVIHFDTGWFQTDWQCDYVFAPDRFPDAQKMINDLL
                     ADGFHISLWQLPYFTPKNKYFPELIEKGLYVKNQKGGLPYEDVVLDFSNPETVTWYQE
                     KLEGLLKMGVGAIKVDFGEGAPLNGIYANGKGGLYEHNLYPLRYNKAVADITRITKNE
                     TIMWARSAW"
BASE COUNT         4322 a         3237 c         3564 g         3822 t
ORIGIN      
        1 gtgtcagcga ccgcagcggg cagcaaaagc acgcagcgcc tcggcgtacc aaaaagctct
       61 ccgttccaaa tggtgtcagc gaccgcagcg ggcagcaaaa gcacgcagcg cctcggcgta
      121 ccaaaaaagc tctccgttcc aaatggtgtc agcaactgta tcaggcagca aaagcacgaa
      181 ccgcagcggg cagcaaaagc acgaacaagg gaaagcctcg gcaaaaagaa aattgcgtcc
      241 cgtacgcgtg gtgtcggaga actgaaattt ttacaaagaa gcagtgcttt agggctatat
      301 tggtccggac ctggatcaaa gataactgcc aggagcagtt tggctggttt tttgcttttg
      361 gtcagcctta aattaacccc accgggtgca gtttccccgg gatttttgtc ccaggtgaac
      421 aaaaatgact aaatttaaaa aaccatttat gatcatgaaa agaatacata acaggaatat
      481 cttgtcgggt atagcttctc tgataatgat tattgcggtc ttcatactgc tatccggttg
      541 catacgcaaa tccatgcaaa aggaagtgga acgggttgtt gaagaagtac tggcatacag
      601 tgccaggacc atagagcctg agttgaaact gctaagggtt aaattggaca ggagccttac
      661 agataaagag aagcagctta tagatcagta cagggaccag gcaaaggtca tgatcgacag
      721 catgataaat tttcaggtta agctttctga actggagggg gaaaaagagt acaggcttac
      781 agaggatgaa atatcctatt ataacgattc tcagaaaaag atgcgtcaga taatcacttc
      841 ctgctgggca gtactggata accacaatga agcctttgct ttgctccaaa gggagctgta
      901 tccccaaaca gttacctggc aaaaagaagt catggaaatt gtgagcacga acctgcctgc
      961 aggattccgg gatgagtttg caaaagaggg cagcggaatt aaggtaatag gaatggaaca
     1021 attatcgcct aataatatgc aacaggtctt tttcatcttg tacgagccta agactaattt
     1081 tccgctcaac aaggtcaagg atatgctgca acgtatgctg gaagacaatc gttgaaagat
     1141 gatatggtgg actaccttaa gcattttttt actacgcaaa gcattccctc taccttgctt
     1201 tacttgtgct ttgccgcctt taccggttca atgctcggga aaatcaaatt caaaggtatc
     1261 gggctgggtg ttgcaggtgt tcttttcact ggcatacttg tcacccattt tggtgcaccg
     1321 gtagattcac atacgttgct ttttatacgc gacttcggac tgatactgtt cgtttacagc
     1381 ataggtctga gtatggggcc gcgctttttt tcctcgttca gaaaagatgg attacttctg
     1441 aatttgtttg ctgtggcaat agtcacagga ggtttcggaa tagccctcct gatatgccgc
     1501 tttaccgaca tgtcgccttc tgccgttgcc ggactcctga gtggggcagt aaccaatacg
     1561 cccagccttg gcgctgccca ggaagtgctt gcagaacaag gtgccggcgc agctgttatt
     1621 gcagaaacgg gcatggccta tgccatcact tatcctttcg gcattttggg ggtgataatt
     1681 tcgatgatat tcatcagatt gttattcaaa ataaacaccc ggtcggaaga ggaggattac
     1741 accaaatcgt tgggcagcag tgaaaaaacg ctgcaaaacg ttcagattca tgtaatcaac
     1801 cttgatttga taggtaaaaa cattgcttac atcaaaaaga tgctagacaa tgaattgacc
     1861 atttcctgta tcaaccgcaa tggggaacac atcgcagcag aagatgaaga aatcataagg
     1921 gagggagata ttatttgcgg tgtctctgcc caggacaaga ttgaaaacct ggaattcaaa
     1981 gcaggcaggg ttactctggc caatattatt gaggatatcc ccggtcccct gggcatgatt
     2041 aatatcctga tgaccaatca taagattgca gggaaaacta tagaacaaat aggcatttat
     2101 cgccgttatc ccgccaacgt gacacgcata taccgttccg gcatgaaaat cctgcctacg
     2161 ttgcaaacaa cactggaact gggtgacaca ttgaggatag tagggaaaaa ggaaatactg
     2221 aacaaggtaa aagacgaact gggggattcc atcaacgaac tggtcaagcc caatatcatc
     2281 tcaatttttc ttggtatttt caccgggatc atcctgggta gcattccaat atttattccc
     2341 ggtttgccag cacccgccag attaggactt gccggaggtc cgctgctgat agccattatc
     2401 ctgggatata agggacggat agggaagctg agtttttaca tgacacccgg agccagcctc
     2461 ataatgcgtg agatgggaat tatcctgttc ctttccagtg tgggattact atcgggggga
     2521 aaatttttgg aaaccattca acacggaggg tggcaatgga tgctatacgg aatagccatc
     2581 acccttattc ccattatggt catgggcatt attgcacgct tgatgaaagt gaactacctt
     2641 aagatatgtg gtatgttggc cggctccatg actgatccgc ctgcactgga gtttgccaat
     2701 ggaattgcgc ctgttcaggc tcaagcttct gcttacggta ttgtatatcc gcttacgatg
     2761 ttccttcgtg tgctcctggc acaggttttt atcctgatga cgttgtagtt tttaaaaacc
     2821 tgcagcaatg catgataaca gacggccatt gaccaggtcg ctgcggttat ccatgaaggt
     2881 agaacattct ccacagatat ctacgcccag aacaggataa ttgcacatga tatccttgag
     2941 gatcgtgtga acctggctgc aggacatatc accctggtcc cagttggtcg ttacgaccct
     3001 cctgtgaaac acgtccttgt ctatggatat gtaagcgggc agggacatga ccggcatggc
     3061 aggataacgg aaaatcctga catcattgcc atccagcggg tattcccctg acacgccaat
     3121 gaacaaaacc tgtttcagga aaggatttgt ttcaagaacc gtcttgaccc agcctccaca
     3181 ggagaggtga tcctcaaaca taggaggctg catgtcatga tgatggtccg cgactaccag
     3241 catgaaatcc ctgtcaatcc tgtctgtcca gaattttgta atgtaatggt ggttgcctgt
     3301 atcaagaaaa tgcacagccc tgagcggatg agcggctatc cgggcctgta ttgtctttgc
     3361 agcgtcctgg ttgcaataac aatccgagcc gggaatatcg gaacaatcta ttattatgtg
     3421 gtcgtaacca agataaaaag actggtttct gtaaaccctc gtaaagtcca taattatgac
     3481 aggaatgttc ataatttctg acattgaacc ttattttgcg aatataccta aaaatacttt
     3541 atatagtaat tatctatata tttgctttgg tcaaagattc aaacatatgg tacaaatgcg
     3601 aggattggaa aagggtaaag agcctttgaa agcggttttt ccaggcaagt atatacaggg
     3661 aaggaacgcg ttgtcagatt tgccttacgt tttgcatgta atgggagaaa acggattgtt
     3721 gctggcctca ccatcggtga tatcaaatgt actcccaaaa tattctttta ttgaaagccg
     3781 gaagaatatc cacattgaaa ctttcggagg tgaatgctgt agccgggagg tagaaaggct
     3841 gtcaggggcc gttcatcata ctaaagccga tgttatagtg ggaatgggtg gaggaaaggt
     3901 gattgatacg gcaaagatcg ttgcagacaa gacgggaata cctgttattg ttgtccccac
     3961 gatagcttct accgatgccc ccacgagcgg ttgtgccgta ttgtattcac cggaaggtgt
     4021 ttttgactct gtatattacc tgaaaatgaa tcctcaggcc gtactggttg acgaaacgat
     4081 catagcttct gcacctacgc gttttctggt gtctggcatg ggagacgccc tggccacctg
     4141 gtttgaggcc agatcgtgca aacgttccgg ctcatctaac gaatgcggag gctacaccac
     4201 cttgacggct atgggaattg cccgtctttg ttatcagaca ttgctgcgtt atgggatgca
     4261 ggcaaaaaaa gatgccgagc tgcaccttac cacccccgca ctgtcctgca tcatagaagc
     4321 caatatcctg atgagcggac tgggattcga gagttgcgga ctggcctcgg cacatgccat
     4381 acacaatggt ctgaccgccc tggaagaaac gcacgctttt taccacgggg aaaaagtggc
     4441 tttcggagtg ctggccggcc tttacctgac aggggctcca gtaaaggaaa aggatcgcgt
     4501 atacggtttt tgtgaatcgg tgggattgcc caccacattt gaagatttga atctggaaaa
     4561 ggtgagccgc acaaggctga tgaaggccgc tgtcaagacc tgtgatccga aagaagccat
     4621 tcaccacgaa acacgaaagg tcactccgga agaagtcctg gcagccatgt tggctgctga
     4681 cgattacggt aaaaagagaa aattgggagc ctatggcaga tagtcctgaa aaacaggaat
     4741 tgctgaagag ttattttgtt aactattcac ccaggttgac aaattatgcc gtacgttttg
     4801 tcacaaacag agaaacggcc caggatattg ttcaggactg ttttatgcat ttgtgggaaa
     4861 agatggagca atttagcttt atccacatta aagcccttct tttcaccatg gtgcgcaaca
     4921 gatgcctgaa ttacctgaaa cacagggatg tagttgatct ttatactggc cgttatcttc
     4981 atgaagttga aggcgaggaa agaatatatc attacgattt tgattttggt cccgacaagt
     5041 tgccactcta tgaagaactt gagaaacagc ttcagatagc agtggattcc ttaccggaaa
     5101 agtgcaggaa ggtttttgtc atgagccgtt ttgacgggtt caagaaccgg gaaattgcag
     5161 aaaaattgag catttcaacc acagcagtcg aaaaacacat cagaaaagct ttaactacat
     5221 tgacttccta tttcgaaaag caatatcctt ttcacatttg catcatcctt ttctcctgtt
     5281 tgctatcctc ctacctggat tagctctcct ataaaaaaca tatttatttt aaaaaaacca
     5341 taaccaaggt taggtaaact tacattttgt tgtttaatat atgaaatcac ctatcagtat
     5401 gaaatcagat caggaaaaaa tcaaagaatt ggcaatcgcg tattttgaag gaaaaattca
     5461 tttgaaagaa gagggcctgt tatttgattt cattagtcaa tcaaaagaaa acaaatcact
     5521 tttcagaaaa tgggagcatg attggctcct ggaacataaa cccgaccagc agaccttcat
     5581 gttatgggac cgtctttcag ccggttatag tagcagaaag aaacaactac taggctctcc
     5641 catagtacgt aaaaacatct tataccgaag gatattccag ggagtggctg cggcagctgt
     5701 tttagctgtc gttttcctgg ggattgaact gggaagacga tccgggcagg atgggggtta
     5761 tgcgtttgtg attgaaacac agaatgcgga acaatccaat gtaatccttc ccgatggcac
     5821 cgtggtgtat ctgaacgccg caacaacact caggtgccct aacagataca atcaaaagca
     5881 cagggtcatg gaactggagg gagaagccta ttttgaggta gcaaggaatg aggatatgcc
     5941 ttttattgtc aaggcaaaga atattcccat aaaagttttg ggaaccaggt tcaatgtgac
     6001 agcctacgca gatgaaccca cagtgacaac agcattgctt gagggcagac tggagatcgg
     6061 gggagaaaat aaaaagatca ctatgaatcc cgacgatatc atccgttttg acaccaatac
     6121 gcagaccgtt tctaaattca ggcaaaacgc atcgcaatat atatcttgga cagaaagaag
     6181 gtttgaatat aacatgatca catttgacga gctctttaaa cggctctcgc gtcagtatga
     6241 tgtcaatatc atttttacag ctgataagaa aatggacaag attttcaata tttcccttaa
     6301 taatggggaa accattaccg atgttctgga tgctctttct ttgattattc catttacgac
     6361 aaaacgagat gggaaaaacc tttatataac attcaaataa ataattatgc attttaacct
     6421 aaaccttaaa agacttgtac tcacttcagg tttgatcctg ttgggggtat tttctttcgg
     6481 acagacgatc accagggagt tcgtgaacgt gtccttggtc aatgttttga aagaagttga
     6541 aaaacagact ggtatgtcta ttatttacga aaccagcgag gtcaatgaat cgtatctggt
     6601 taccgccaga tttgaaaatg catctgtaga agaagttcta cagacggtac tcgagggatc
     6661 gttatattat tccattgaag gcaagatcat agtgcttcac aaacagcctt ctgaaaaaga
     6721 caaatccatt ccccaggttc aacagcaaca ggaacatcgt gtaaccggca ctgttgttga
     6781 tgagtctggt ctttcgatta ttggagcttc gattgttgcc caaggatctc agactacggc
     6841 cattacagat ttggacggga attattccat tcgtattccg gctgatggcg tacttgtagt
     6901 ctcctacatt ggatttgtaa cccagaccat tcctgtcaac aaccaggcag aaatcaacat
     6961 agtaatgagt gaagaccgtc ttgtacttga ggaagtggtt gttgtgggct acggtactca
     7021 gaagaaaaaa ctgctcacag gggccaacct gaacattaag gaagaagata tcactaaaag
     7081 aagctcccag aatgctcttg atgccctgat tggccagagc cccggggtac aaatcatagc
     7141 ttcttcggga cagccaggtg aagatttccg tgtaagtgta aggggtctag ggacagtagg
     7201 tgatgccaca cccctatatg tggtagatgg agtacaggta agcggtatat cccatatcaa
     7261 tccttcagag attgaatcca tcgatgtgtt gaaagacgcg gcttcggcag ccatttacgg
     7321 cgcacgggca gccaatggtg tgttgctggt aacaaccaaa aaagggaaaa ccggtaaaac
     7381 agtggttgga tatgatggat ttatgggagt tcaatccttg gtaagaatgc ccgaaatgct
     7441 caatgcacag gattatatga tgatcatgga cgaggccagt atcaattccg gtggtacagc
     7501 ttatggctgg ttaagtgaat tcaatattga tccggccaag atcgggcagg gaaccgactg
     7561 gctaaaccag ataattgtga agaatgcgaa aacccagaac cacgtggtta gtgtcagcgg
     7621 tggttcacag ctcagtgtct ttgccttgtc tttgagttac agcggacagg aaggcgtgat
     7681 aggagggaag gattactcca ataatgaacg gtttaatttc cgcataaata gtgaccataa
     7741 aatttacaag gatattgtaa aaataggtga gaatctctcc ttgtccttta tcaaccggca
     7801 tggagttctt atcgggaacc agttcaataa tatcataaat gacgcattaa gagccacccc
     7861 tgttttaact ccctataatg aagacgggac agactacgca tcggcaacat tatggtatgt
     7921 agatgaaacg aatccattgg catcactggc tctgagaaat cagaaaaaaa cacagacggc
     7981 ccggttgtca ggagatattt acctggaggt tcaacccata aagaatttaa agctcagaag
     8041 tgtctttgcc ctggattaca gtaattccaa ttcccggagt tatacaccgc aatattatta
     8101 cggaagtggc ttttacaacg acaccgatta cgtgattcag cagaatgcgc tcagaaccag
     8161 ctggtcctgg gaaaatactc ttaattataa tttcaatctg aaaaatcata attttgatat
     8221 tttacttggg attcaggcca ggcaggaaac cggtcaatac ctgtctgccc agaaatacga
     8281 tctggtattc aacgactttg accatgcctg gattaacaat gcgaccaata ccaatgcgaa
     8341 tatggtcatt gttgagggct acccttacaa cctggataac cttgtttcat atttcggtcg
     8401 tatcagttat aactataaag agaaatacat gtttaccgca acggtcagag ccgatggttc
     8461 ttcaaaattc ggtccgacca atcgctttgg tgtatttcca tctgcatctg cgggatgggt
     8521 gataagcagc gaaggtttca tggagggtgc gaaagatgtg atcagcttcc tcaaattgag
     8581 agccagctgg ggtcagaacg ggaataaccg tataaccgat tacacgtatc tctctaccat
     8641 agctaccact tcttatgctt tcggagataa ggacaatcaa acatccacta caacaggtgt
     8701 ctatgaaaat tccatgccca atgtaaacgt aaagtgggaa acatccgagc aacttgacct
     8761 gggtctggat ttgcaattgt ttaacggaaa aattcaggca gcttttgact attacaacaa
     8821 aacaacaagg gattggctgt tacaggccga tgtgctggac gtgttcggtg ctcagacaaa
     8881 cccttacatc aacggtggtt ctgtaagaaa caagggcgtt gagttcgggg catcttattt
     8941 tggtcagata gggaacgaat ttcagttttc cattagcgcg aacggcgcgt tcaacaaaaa
     9001 tgaagttata tccatcccca atgtagaagg gatcatacac ggatcccagg atattctgtt
     9061 caagggaatg caggaaatga accgtgccca ggaaggttat cccataggtt atttctgggg
     9121 attgaatatg ttagggatct tccagaatca gagccagatt gacaaccata aaaattccga
     9181 aggcaaagtg attcaaccct atgccaagcc gggagatatt atctatgacg acaccaacga
     9241 tgacggagat attactcagg cagacaatat atacctggga caaccttatc ccactactac
     9301 gattgggtta aatctgtccc tgaattacaa aggattcgat ttctacctgg ttgggaacgg
     9361 ctcttttgga atgcagatcg caaaatgtta ccgtccaata gaccgtaaac aatacaatta
     9421 taccgctgat attctgggcc gctggaccgg agaaggaact tcaaacacca ttccccgtgt
     9481 gaccaacggt gatgaagata atgggaaccg tctgtatatg tctcagttat atatagaaga
     9541 tgccgatttc ttccgtatta gcaatgttac acttggatat aactttgccc gtttattcaa
     9601 gcaaaagaca gtcatcagtc agttgcgtgc ttacgtaacg gttcagaatg cactggtcat
     9661 taccgggtat tccggaatgg atcctgaagt cgggtacagt ggcggttcta catggggatc
     9721 tggtatagac ctaggctatt atccacgtgc ccggactgtc ctctttggtt tgagtattaa
     9781 actttaaaaa tgacggagat catgaaacat atattaaaac tagcagcagt aatcttagta
     9841 tcagttaacc tgtcatcctg ttcagatgat tttcttaaaa gtgaaccgat gactcaactg
     9901 acttcagcta acttctataa aacccccgaa gagcttaaca tggccctggt tggttgctat
     9961 cagcagctga ggggaggcta cggagatttt ttccagttgc tgaacattgc cgccgatgat
    10021 tgttacgggg gaggcggata taccgatgct tacggtaacc agatatggga tgagtttact
    10081 cattacaatg acctggaaat caatcgttac acttgggctt caaaggtctg gcgcgctatc
    10141 aggagatgca acgtggtggt agaacaggca gaaaatattg attggggtgc cgattccaaa
    10201 ctgaaaaatc aatatgtagc cgaggccaga atgctcaggg cctactggta ttacttgaca
    10261 caagcttgtt atggtcacat tcccctggtc acaacatcgg atcagcttga aattgaagga
    10321 caggcagatc ctgacgatgt atacgcctat attgcagaag atttgaagtt tgctattgag
    10381 aatcttccgg caacccgttt ccaggatatt tccaagggtg agattggcca tgtgaccaaa
    10441 tggtttgccg agggatttgc cgccagggta taccttttct acaccggtta ttatggcaaa
    10501 agtgaaatgc ccggtctgac caaagcccag gtgacaggct atttgaatga tatcattacc
    10561 agcagcgggc acgcattgct tcccagtttc agttctctct ggccctggtc tatgagaaaa
    10621 gattactggt ccaaacaatc cctttacgtg ggtaatccca acctggtgta tgccggagag
    10681 tacaatcccg agatagtatt cgccattaaa ttgcatacag gattctcctt ttctcattcg
    10741 caatatatgg gcatgagggg agaagtaaaa gcaggagatt atgacccgtt tacttccgga
    10801 tggggaatgg ctacaatcac tcctaaggct tacaactggt ttgaagaagg tgatacgcgc
    10861 cgttttgcct ctatcgtcaa tctggctgct gaagggatcc cctgcgacgg aacggatcaa
    10921 cgtgaattta ccggttatgt acataagaaa taccaaaacc tgacttctcc cgttgaccgc
    10981 acaaaaggtt atccgcagta cctgggcgcc agcaataaga acgccaatcc ccaggaccag
    11041 attatcatgc ggtattcgga cattctcctg atggctgccg aactggtggt ggacacaaat
    11101 cctgcacagg ctctcaatta cgttaaccag gtccgcaaca gggcatttga aaacacttca
    11161 catgcgttca aatcggttac caaacaggat atcctggacg aaagggcaag ggaatttgtt
    11221 tttgaaggcc tgcgttacca tgatgtgctg cgccagggac tcaccaaagc aaaacaaatc
    11281 cttgatgtaa aaggagaaat cgtattaaac gggaaaaatc cggcaacaaa aaccatcgac
    11341 tggcctattg aaaaagaagg cctgttaatg atcccttata gtgatgtgtc tttgtcaaac
    11401 gggaaactcc ttcagaatcc gggatggtaa ctttttaaat tgaaaaaatg aacaaaaaaa
    11461 tactcgtttt aatcaccctg ctgtcatttc tgggcattaa ccttcaggca cagcaacctg
    11521 cactgaaaaa gaaaggggat gccacccaaa tgtacgtcga cgggaaacct atgctgatgc
    11581 tggccggtga actgggaaat tcttcaagtt ccagcgaagc ctacctgaaa gatgtatggc
    11641 ccggactaaa ggcgttgaat tacaatacaa tacttgcagc cattacctgg gagatgatcg
    11701 aacctgaaga aggtgtattt gatttcagtt ctgtagacca cctgataagc agcgcccgcc
    11761 agtacgattt gaaactggtg ctgctttggt tcgcaagctg gaaaaatgcc gcctccacct
    11821 acattcccct ttgggtcaaa aaggattatc agagatttcc ccgggccgaa gatgcaagcg
    11881 gaaaaccggt ggagattctt tcggctgttt ctgaggtaaa catgaaggcc gatgcccgtg
    11941 ccttccgttc cctgatgaag cacattgccc aggtggacgg taaagagcat acagtgctaa
    12001 tgatgcagat agaaaacgaa ccaggcatac tcaatacccc ccgggattat tctcccgaag
    12061 ccgagttgct tttcaatggc cctatacccg aagacctggt tcaatatctt aaaaagaaca
    12121 aaaacgacct gagcagagac ctgtcaaaag tatggagtgc caatgggtcc aaaactaagg
    12181 gaacctggga ggaagttttt ggcaagagca agatcggtgg atggagccat accggagaat
    12241 ttgactggca cgacatgtac tactatacag aggaactgtt catgggttat tattatgcca
    12301 gatatatggg ttatgtggca gccgaaggga agaaggaata tgacattccc atgtatgcca
    12361 atgcctggct caaaggtccc gattacggct ggtccggaat gcatccgggg ggaggaccgc
    12421 ttcccagcgt aatagatatg taccattgtg ccggaccggc cattgatatt ttatctccgg
    12481 acatttatgt acctcatttc accgaaattg tagagtggta tgatcaattg ggaaatcctt
    12541 tgtttattcc cgagaccagg ggtggggagc tgggtgtttc gcgcctgttg tggtgccttg
    12601 gggaacacaa tctgatgggc ttctcacctt ttggaataga ccgcagcgca aggaccggcc
    12661 ttaccagtgc ccaggcagat cccctggcac aggcatacaa actcattgac ggaatgcagg
    12721 aggtgatcct ggaataccag ggaaccggca atatccgggg tttctttgta gacaatgaca
    12781 atcctgaaca atcttttgta ttaggtgagt atattgttac agccgacctg atgcgcccca
    12841 gaaactatgc ctccatgggt gctcccgctc ccaccggagt gaggactgtg cctgaaaccg
    12901 cttcaggagg cggatttatt atccttcagc ccaacgggga atacatagtt atggcccgca
    12961 atgtcaatgt caggtttgct ccggcccaac cggggaacct gccatacata ggtttgggaa
    13021 ctttaaccga aggagtattt gagaatggag aatggattca gggaagggtg ttgaacgggg
    13081 accagatcca tggcagcata tttaccggaa ccgggctgaa aatgaacaca ctgggcattc
    13141 agagagttac attgtaccgt tacggtaaaa gagaaatcaa gcagtgatag tttgattaat
    13201 tagtttttta ggttagatga taatctttca cctcacctta ctgtgaggtg aaagatttga
    13261 aatctagagc cacataggaa actatacttt ttatacttac atttattcac ccgttcatga
    13321 tctggcagta tttcagaacc cgttacggag catgacaggt aaatccaata acaattaaat
    13381 aaatgtgccg tttatgaaaa gattactgat attttacggg gtgcttttac tctgcctgca
    13441 agcgaatgcc cagccttacc ttcaaagctg tgccgttgat gtaagccagg atttcacaga
    13501 ttttagcaat acgtattttt ttgccgattc tctggcgtca ttcgatcccg ccaccgcaga
    13561 agggtccatc cgctggaaac gttacagcct ttttggaaga caggcattca acaccacaaa
    13621 agtctttcca cttcctctgg agatgctgga ttttccccct acccagtacg aaaatgaccc
    13681 gcagttgcgt ttcagcattg attttgtttc accggtcacg gtacgactga gggtatacac
    13741 ttctccggtc atactttcgg aagaagaatc actcatgcta acaggaacac cgccccggga
    13801 cggatcctgg acctataaaa aggggaatgg cacacacacc tatacaggcc ggggagggag
    13861 cattgtgatc agggagtatc ccttctccat catgctctgc gatgagcgcg gcagggaaat
    13921 gacacgcacc aggcactggg ccgataatga ttccacccag atcaaagtgt tacccttcca
    13981 gtttatcaaa agagccagtg acaacatccg gagtatcaac cccgtatttt ccctgcggcc
    14041 gaacgaaaaa atcgtaggat gcggagagtc tccgactgcc ctgaacaaag tcgggcaaaa
    14101 agtgcattta tttgtgaccg atcctcaaag tccggaagga gatcagatgt acaagccaat
    14161 tccattcttt atcagtaaca aaggatacgg gatgttcatg cacacctctg caccggtcac
    14221 ctgtgatttt ggggcaacac atgccggatc ggccaaattg ttcatggcag acgaaacgct
    14281 tgatcttttc ctgttctggg gaaagccggc cgagatcata gaccagtaca ccaaccttac
    14341 cggcaaggcc accatgcctc ccttatggtc tttcggaacc tggatgagcc gtataaccta
    14401 tttttcacag gaagaaggct atgaagtggc cagaaagttg cgagagaata aaatccctgc
    14461 agatgtgatc cattttgaca caggctggtt ccagaccgac tggcagtgtg attatgtatt
    14521 tgcccctgac cgtttccccg atgcccagaa gatgatcaat gacctgctgg cagacggatt
    14581 tcatatttcc ctatggcaat tgccttattt cacccccaaa aacaaatact ttcccgaatt
    14641 gattgaaaag ggcctgtacg tgaaaaacca gaaaggaggc cttccttacg aagacgtggt
    14701 gctggacttt tccaacccgg aaacggtcac ctggtaccag gaaaaactgg aaggattgct
    14761 caagatgggg gtaggggcca taaaggtgga ttttggtgaa ggagcccctc tgaacggcat
    14821 ttatgccaac ggaaaaggag gattgtacga acacaatctg tatccattgc gttataacaa
    14881 ggcagtggcc gacatcaccc gcataaccaa aaatgaaacg atcatgtggg ccagaagtgc
    14941 ctggg
//
LOCUS       DAOSOF010000037        14840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4063_length_14840_cov_5.616977, whole genome shotgun sequence.
ACCESSION   DAOSOF010000037 DAOSOF010000000
VERSION     DAOSOF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_14840_cov_5.616977"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2804
                     /locus_tag="PLM68_03500"
     CDS             <1..2804
                     /locus_tag="PLM68_03500"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOT54463.1"
                     /translation="PYMFSDELGGAAIGAVTAIGGETALQLLLGAPLRAETITGLGKL
                     GSPLAEKMLMSLAQTGNEQQRPLAFQALSRCGTMAAFDVMANAPAFDFLTFLERVAPS
                     DPGKATKALKSLIKPGNDSQTRCFALSLLIGLHQEEAMRTVLRALDDADGSYRRTALE
                     GTRTFISPVVNSAVLKKMKRLSAPALADVLDWLGEQRNPALIPYIADPWLNHEELQVA
                     EASARAILKTGTEQGTALLAGLLTGTDPLRTDLALKSLLASEGNVIQAVLDIYPQTSV
                     PGKSAALALLGARKSRANEPLVLEALQDSSPEVRLAAALALSGVARPDNRDTYFRLLE
                     AAAPELKEPLQQAVMASLYGLDPEAQFNVLSARMQQAGPLLRPLYYAPLAATGTKRAV
                     DLLSERYLEEGNADAYITLINGWNPTGAQKVIYLRKGLDHFTEPGQTAALLAQMKNTG
                     AFQAMVASAPYLDSAHPEVSLAAAMNIYHLAVNNPDFYGPLVAEWMEKVMRIMEQAGY
                     PDSIYDIQNVKKYLAETPEEKGFERIFNEKDLEGWQGLVGNPISRSNMKPQELAKAQK
                     EADKVAFSQWVVEDGKLLFLGKGDNLCTTKNYGDFEMYVDWLLYPEGPEADAGIYLRG
                     SPQVQIWDTARVHVGAQVGSGGLYNNVKNPSVPLLVADNRLGEWNSFFIRMQGERVTV
                     YLNGELVVDNVVMENYWDRSQPIFPYGAIELQAHGSKVAYRDIYVRELQRPEPYKLSP
                     EEEADGFTVLFDGTTLHQWTGNKTDYLTRDGVLEVRPTGQGFGDLYTAKDYSDFVFRF
                     EFKLTPGANNGVGIRTPGTGDAAYEGMEIQILDHFDPIYQPWLLDYQYHGSVYGVIPA
                     HNRDALRPVGEWNQQEIYVKGTYIRVTLNGEVITEGDISAGPVDEREHPGLERTGGKI
                     GFLGHGSRLWLRNIRIKEL"
     gene            complement(2813..3196)
                     /locus_tag="PLM68_03505"
     CDS             complement(2813..3196)
                     /locus_tag="PLM68_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54464.1"
                     /translation="MKKKGIRMIKGLMALSLLILMTASCQKEYKVSDLLGTWAYQDNT
                     QYTITFREDGTMGYTAPVSAPSYTPTYTLDGFYIYVRVIVGSATINDVVEIRSLTPTT
                     LVVYYDGDLLFDGQSLDGEYTLLKR"
     gene            complement(3216..4055)
                     /locus_tag="PLM68_03510"
     CDS             complement(3216..4055)
                     /locus_tag="PLM68_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT54465.1"
                     /translation="MKPLKYLLYMLPAVVMLLQVSCSQGPDTVQVMSYNIRLSLVDDG
                     ENHWNIRKPASIIMIDSLKPDVLGVQESVKEQVDYLVEHLPAYARVGVAREDGDSIGE
                     FSAIFYLKDKYEVMQNGTFWLSETPDVPSFGWDAAHMRIVTWGKFRNRKTGRTFFAFN
                     THFDHRGEVARRESAHLLVQKIGELTLGAPVVFVTGDFNAEVSDPLFEPVKAHFLDAR
                     QEAPITDTLSTYNGWGTYNGAPIDHIFYRGAKALSFRTVTDNFGVPYVSDHYPVLAVF
                     EYE"
     gene            complement(4073..7006)
                     /locus_tag="PLM68_03515"
     CDS             complement(4073..7006)
                     /locus_tag="PLM68_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOT54466.1"
                     /translation="MVKNLFLPIFILTIMLAFSGCKKEDMVYYINSVPQELHFTHEGG
                     LDTVAITSNSGWNVNIPLDWCRTNLSSVGTSEKTVLLSFNVETNTTTQERSQDIVIKS
                     TNNASIQSVIKVIQDAMEDPEDPDDPDLADTLFTTPVSMEVPCKGGNYDFTLVSDTTW
                     VYDSSNAAWCNLVSEQSSGNRGEYRMTFSVEPSQLTQARTALLTFKTSNDSLAILEVT
                     QRPLGISVLEDLLLFRDDVNAFADLRLWMDSDSTIHLLSDLDLSSIPNWIPIGLHTNA
                     MIYSEDNSSITGVFNGNNHTISNLRITQNSSRSAGLFGYVRKATVQDLTLDHTCSITL
                     VPGQFQSMSAGGICGTMLGGTLTGCHFQGTIQISGLSTSTATGGIVGEMRADENLKAA
                     VVTECSNGGTIQGLSPVGGIAGRSNGSSIVSCENNDDARVKGSLAVGGICGQSWTSAV
                     INSSHNRGSVEGTGEKTGGICGELYSLAVVSNSVNHSGAIVRGTSRIGGICGYSASSS
                     QIDNCVNEVGLSAVSEAGGICGTQYLDCSINGCSNSGSITASGSDENKNTGFGGITGG
                     NFYSEITTSQNSGTVKGPSSIGGIAGFSNNVVKDCANTAPVEGVIFIGGTIGTADGTG
                     YTLSFLTNSGTVTASSGAGGIIGYITGTVTVSFCTNQESGVVSASAGSAGGITGVSGT
                     VQYTGTVLSDCENHAPVSSARRAGGIVAVSYGKINDCLNAGVVTVPMTDDPVEETEEI
                     EINNIAMAGGIAGISSSLIENCENQGAVNGYTAGGIVARFTASSSLYKLKNCTNSGQI
                     TGIRSAGGVAGSITKGGTAETLVNSGNVTGSYSVGGVVAENVKGSLTDCGNSGTIQGA
                     ETEMDATFFALGGVCGMNDSGNMTNCSNSGAVNRIAQTGQYRYVGGMVGVTGRGTGTG
                     GALKSCSNSGPVSGYVSDDEQDYCYVGGFCGLFASGPAPEDCTNTGTVNGQPASDENM
                     YGGTN"
     gene            7074..10157
                     /locus_tag="PLM68_03520"
     CDS             7074..10157
                     /locus_tag="PLM68_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOT54467.1"
                     /translation="MKTRWRILLVVLLSVLGLVAAIMTILSIYMTPSRLTTLINQYAG
                     QYMEASVTISDAAIHVWKDFPYISLELADGEILVPAPDTTGKWADSLLVFKKMEVTLR
                     PAALLRRMVVIPRLHLEQASIHLFTDLEDRSNWDIFPGSGDTESPSYGLSFGRLSVRD
                     TLRFTYHDYKDTVYYSVDITGMTARSPFRGWAFTADIQSCSNTLIVGENLLFDRIPFN
                     VKGDIVLENDFDDYYFRNCETVLGKVPLFINGFMGLREDSLELRGLCLIDSTMLTDLY
                     DAFPLNLLPREKPFASSMPIKDLELFLNGAYKYESGEFPVYWAHLEAGPGSLVHLPSG
                     IMIPEIKTRIASSYDPGENNHGILEMEDLTVRSPALDLSVNGILSSVFKEPSVRLSAG
                     IEARLDRLHELVEVVEEGRAVGGVTANLEADFRFKDLNPAALGNVMLSSDFQTDGFTL
                     DLPAYSLFCRADSTRLRLGIHPGTSLDLELVSDSLQILYKGRELAALSGTELKAGSTP
                     GMFTMDSTKVQPLNGSLKSRNLRVYVNDSTRIGMDGGNIVFSVKSDSRDQSLPVIGLT
                     LESRSVGAMMGIHRGSISDLHLQADLTRNVRTQHRDTAGLSARRDSFLLRRARPRHEF
                     SYADLSLDLDREERGLLRRWNVNGTLQAGGIQLITPFIPLNTQVRDAHLVLQNDRLEI
                     THTVIRAGRSDLLLNGYMTDMRQVLLGRGRLGLNFDLQSDTLDCNELIGAINSGMSLM
                     EKQDSLPGSTEIPAYQNLLVVPGNVNLQLVLAVSNAYYLHLQMKGLHGMLLAADRYLQ
                     LDELQASSNAGDITIDALYATPSRDSLQVGLHMEMKDILLEEVTRLFPSIDTLFPMIR
                     SFRGLADIQFSATSQLDTDMNLVLSTLRGACRIAGNGLVLLDGETFSEISRKLMFRKK
                     ALNLIDNIRVEATVADNRVDVYPFIMEIDRYRAAIAGSHNMDGTFHYHISLLKSPVPF
                     RFGIDVLGVPEDYKIDLGRARFTDQGIPALSYRIDSIRVNLREQIKRYFENYSFE"
     gene            10159..10914
                     /gene="kdsB"
                     /locus_tag="PLM68_03525"
     CDS             10159..10914
                     /gene="kdsB"
                     /locus_tag="PLM68_03525"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR00466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOT54468.1"
                     /translation="MYTIALIPARYASERFPGKLMQKLGDETVILRTWRATLETGLFR
                     KVVVVTDSEEIYREIVSHGGEARMSTGKHLCGSDRIAEAAEHYPQADVIINVQGDEPF
                     TSAGPLKALLGVFEEAGAESVDVASLMSPLGETEEPDNPNVVKVVTDRQGFALLFSRA
                     PIPFTWDPAGYKAKTFKHIGIYAFRREALLRFASLAPGPLELSEKLENLRFLEYNMRV
                     KMVEVPGYSKGIDVPKDLEEAKKMISPCSGIHV"
     gene            complement(10886..12847)
                     /locus_tag="PLM68_03530"
     CDS             complement(10886..12847)
                     /locus_tag="PLM68_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT54469.1"
                     /translation="MDILRMGKKNEAQYSQLVEEQRDIFLKNAAVDRLPEDLERIRQA
                     YEYAFQAHCRQTRKAGEPYILHPIAVARIVNEEIGLGTNPIIASLLHDVVEDTPHTVE
                     EIRELFGEDIAYLVDGLTKEKKEQYKLSMQVDNIRKMLMTIQYDIRALLIKLADRLHN
                     MRTLADMPLHKQMKITGETDFFFAPLANRLGLYKVKTELENLSFRFRSPEEYARIQKI
                     LDDYAVETQGIRTRFVTPIEQTLREHGINARISQDIRSVYSIWRHMQQRQVPFRRLES
                     VYVFNIVFDVKTEGPCDVSEKNQSLQIYSLLTDLYLERVGSFHNYVDNPKANGYEALH
                     CMFMTEHGTWAEVHIKSRRMEEASIHGCIVKRHKNVGIGGVDGWVEEFRGTLKDMVYH
                     DGFNNKDGFFIEGVKANLYNDDIRVFTPKGEQILLPSNASALDFAYVIHSEIGDHALF
                     ARVNGMLCSIKTVLKRGDRVEIGTSSAIMPQKDWLEAVVTYKAKSCIRSALRKQNISI
                     KPSRYKFAKCCSPLTGDELLGFEAQDGSGKVFVHTRDCERAIKLSTEHGETIVNVEIE
                     ADENTYYPAHIHAFGVDRKGITFEISQIISEEWGLNINNMSVTTRDALFECDIYLEVR
                     SAKEIYNIIKRLESVLGVEEVKRVSLSRD"
     gene            12903..13493
                     /locus_tag="PLM68_03535"
     CDS             12903..13493
                     /locus_tag="PLM68_03535"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOT54470.1"
                     /translation="MAHQLPPLPFSANDLSPAISSLTIGYHYGKHHQAYINNLNNLIV
                     GTPFENSGLETIIAKAPAGALFNNAAQVFNHTFYFNQFKASATAKKRPENELLRAIEK
                     DFGKFEKFVETFSSAAATVFGSGWAWLVLDQEGNLVITQESNAGCPLARKQKPLMTCD
                     VWEHAYYLDYQNRRPDYIKAFWDILDWNVIEKRFGA"
     gene            complement(13494..>14840)
                     /locus_tag="PLM68_03540"
     CDS             complement(13494..>14840)
                     /locus_tag="PLM68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT54471.1"
                     /translation="EQGMESPAFRIGDSILAPLASAALEAFFLQRSTPGHPDTVVLVH
                     ASAASQERPEGTIIASAGGWYDAGDYNKYIVNASYTTGLILAGYEQYPGYALNRRLLD
                     EVMYNLQWSLTMQDPADGGVYHKLTTPEFEAFIKPGACKKPRYVVQKSVTATLDFAAS
                     MAQAARIYRDIEDHSSTAAVMVKAAEAAFCWALEHPDALYNQFRMNEQFTPAIQTGAY
                     GDLSAKDDFFWASCELYLTTGNEAYLDKALEYAPEKFTAPTWRDVYGLGVFALLTHNK
                     ATPAMKEQLIAFCDSVVSLAGGAPFASSYGNSARDFYWGCLSESCCSNAISLLYGFSQ
                     TGNRDYLMQAYHNMDYLLGRNPTGYCYVTGTGSKSPQNPHHRLAATDDIPGPLPGFLV
                     GGPNPARQDGCVYPSDLPDECYLDVVEAFASNEIAINWNATLLHLAIALEELSQLL"
BASE COUNT         3531 a         3950 c         3714 g         3645 t
ORIGIN      
        1 ctccgtatat gttctctgat gaactgggag gggcagccat cggggccgtg acggccattg
       61 gtggagaaac ggctttgcaa ttgttgctgg gagctccttt acgggccgag acgatcaccg
      121 gtctgggcaa gctgggatcc ccgctggcag aaaaaatgct gatgagcctg gcccaaaccg
      181 gcaatgagca gcagcgtccc ctggccttcc aggccctttc ccgttgcggg accatggctg
      241 cctttgacgt tatggccaat gccccggctt tcgatttcct gaccttcctg gagcgggttg
      301 caccctccga tcccgggaaa gccaccaaag cactcaagtc cctgatcaaa cccggtaatg
      361 attcccaaac ccgttgcttt gccctttccc tgctgattgg cctgcaccag gaggaagcca
      421 tgcgtacggt gctgcgggcg ctggacgatg ccgatggttc ttaccgcagg acggccctgg
      481 aaggtacccg cacgttcatt tcgccggtgg taaactcggc cgtgttgaaa aagatgaagc
      541 ggctttccgc tccggcactg gccgatgtgc tggactggct gggtgaacag cgcaatcccg
      601 cgctgatccc ttacattgcc gatccctggc tgaatcacga agaactgcag gtggcagagg
      661 cctcggcccg ggccatcctc aagacaggaa cagaacaggg cactgcactg ctggccggat
      721 tgctcaccgg caccgatccc ctgagaacgg accttgccct gaagagtctg ctggcctcgg
      781 aaggaaatgt gatacaggct gttttggata tttatcccca aacctccgtt ccgggaaaat
      841 cagccgccct ggcactgctg ggcgcacgga aatcccgggc gaatgagccg ctggtgctgg
      901 aagccctgca ggattcctcc ccggaagtcc ggctggccgc tgcccttgcc ctgagcggtg
      961 tggcccggcc cgataaccgg gacacctact tccggctatt ggaagccgct gcccctgaat
     1021 tgaaagagcc tttgcagcag gctgtaatgg cctctctgta cggacttgac ccggaagcac
     1081 aatttaacgt gctgtccgca agaatgcaac aggccggtcc cctgctgaga ccgctttact
     1141 acgcgcccct tgccgcaacc ggcacaaaaa gggctgttga cctgctgagc gaacgttacc
     1201 tggaagaagg gaatgcagac gcttacatta cgctgatcaa tggctggaat cccaccggtg
     1261 cgcaaaaggt gatatacctg cgcaaaggcc tggaccattt cacagaaccg ggacaaacgg
     1321 cagcactcct ggcccagatg aaaaataccg gtgcatttca ggccatggtc gcctcggctc
     1381 cttacctgga ctcggcccat cccgaagtct ccctggctgc ggccatgaac atataccacc
     1441 tggcggtgaa caatccggat ttctacggcc ccctggtggc ggagtggatg gaaaaggtga
     1501 tgcggatcat ggaacaggcc ggttacccgg acagcatcta cgacatacag aacgtgaaga
     1561 aatacctggc cgaaacacct gaagaaaagg gatttgaacg cattttcaat gaaaaagacc
     1621 tggaaggctg gcagggtctg gtaggcaatc ccatctcacg cagcaacatg aaaccgcagg
     1681 aactggccaa ggcgcaaaaa gaagcggaca aggtggcttt cagccagtgg gtggtggaag
     1741 acggcaaatt gctgtttttg ggcaaaggcg ataacctttg caccacaaag aattacgggg
     1801 attttgaaat gtatgtggac tggctccttt acccggaagg acccgaggcc gatgccggca
     1861 tttacctgag gggttcaccc caggtgcaga tctgggacac tgcccgggtg catgtggggg
     1921 cccaggtggg atcgggcggc ctgtacaaca acgtgaagaa tccttcggtt cccttgttgg
     1981 tggccgataa ccgcctgggg gaatggaaca gctttttcat tcgcatgcag ggagaacgcg
     2041 taaccgtgta cctgaacggg gaactggtgg tggacaacgt ggtcatggaa aattactggg
     2101 accgctcaca gcccatcttc ccgtacgggg ccattgagct ccaggcacat ggtagcaagg
     2161 tggcctaccg cgacatctat gtgcgggaac tgcaacgtcc cgagccttac aaactgtccc
     2221 cggaagaaga agccgatggc tttaccgtgc tctttgacgg caccacattg catcaatgga
     2281 caggaaacaa aaccgattac ctgacccggg acggggtcct ggaagtccgg cccaccgggc
     2341 aggggttcgg cgacctgtac accgctaagg attattccga tttcgtattc cggtttgagt
     2401 tcaaactcac cccgggggcc aacaacggcg taggcatccg tacacccggc acaggagatg
     2461 cagcctacga aggcatggaa atccagatcc tggaccattt cgatcccatt taccagccct
     2521 ggctgctgga ttaccagtac cacggatccg tttacggagt gatcccggca cacaaccgtg
     2581 atgcccttcg gcctgtcggt gagtggaacc aacaggagat ctacgtcaaa ggcacgtata
     2641 tccgcgttac gctcaacggt gaagtaatca cggaaggaga tatatcggca ggccccgttg
     2701 acgaaaggga acatcccggg ctggaaagga cagggggaaa gataggattc ctgggacacg
     2761 ggtccaggct gtggctccgc aatatcagga taaaagaatt ataagcggga aactatcttt
     2821 tgagcagggt atattccccg tctagggatt gcccgtcaaa aagaaggtct ccgtcgtaat
     2881 atacaaccag tgtggtcggg gtcagggaac ggatctcgac cacatcgttt atggtggcac
     2941 tgcctacaat gacacgtaca taaatataga acccatccag cgtataggta ggcgtatagg
     3001 atggggccga aacgggggcc gtgtaaccca tggtaccgtc ttcgcggaat gtgatggtgt
     3061 attgtgtgtt gtcctggtaa gcccatgttc ccagaaggtc agacacttta tactcctttt
     3121 ggcaggatgc ggtcattaaa ataagcaggg acaatgccat cagtcccttg atcatgcgga
     3181 ttcctttttt cttcattgat attatatctt ttttattatt catattcaaa aacagccagt
     3241 accgggtaat ggtcagacac atagggtacg ccaaaattat cggtaacggt acggaaggat
     3301 aatgcctttg ctccccggta aaaaatatgg tcaataggag ctccgttgta cgtaccccat
     3361 ccgttgtaag tggataaggt gtcggttatg ggcgcttcct gtcttgcatc caaaaaatgt
     3421 gccttaaccg gttcaaagag agggtcactg acttcggcat tgaaatcacc tgtgacaaaa
     3481 accacgggag cccccaaggt cagctccccg atcttttgca ccagcaggtg ggcagattcc
     3541 cggcgggcaa cctctcccct gtggtcgaaa tgggtattga atgcaaaaaa agttcttcct
     3601 gtcttcctgt tcctgaactt gccccatgtc acgatcctca tatgggcggc atcccatccg
     3661 aaagagggca catcgggggt ttcgctgagc cagaacgttc cgttctgcat tacctcgtat
     3721 ttgtctttca ggtaaaaaat ggcagagaat tctcctatgg agtcaccatc ttcacgcgcc
     3781 acccccaccc tggcataagc cggaaggtgt tccaccaggt aatcaacctg ttccttgacc
     3841 gattcctgaa ctcccagcac atcgggcttc agagaatcta tcattattat ggaggcgggc
     3901 ttgcggatgt tccagtggtt ttccccgtca tcaaccagcg aaaggcggat gttataggac
     3961 atgacctgca cagtatcggg tccctgtgaa caggatactt gcaacagcat gactactgct
     4021 ggtaacatgt acagtaaata tttcaatggt ttcatttata ggtttttgcg tattaattcg
     4081 ttcctccgta catattctca tcactggcag gctggccgtt gacagtgcct gtattggtgc
     4141 aatcttcagg tgccggtccc gaggcaaaca ggccgcagaa tcctcccaca tagcagtaat
     4201 cctgctcatc gtcactcaca taaccgctta ccggtccgga attggaacac gatttgagcg
     4261 ctccccctgt tcctgtaccc ctgccggtaa ccccaaccat gcctcccacg taacgatact
     4321 gcccggtctg agcgattctg ttcactgctc cggaattgct gcaattggtc atattgccgc
     4381 tgtcattcat cccgcatacc ccacccaggg caaaaaacgt ggcatccatc tccgtctccg
     4441 caccctgtat ggtacctgaa ttcccacagt ctgtaagaga accctttaca ttttctgcca
     4501 caacgcctcc cacgctataa gacccggtaa catttcctga attcaccagg gtctcggcag
     4561 taccaccctt ggttatggat ccggcaaccc ctcctgcact tctgatgccc gtgatctgac
     4621 cgctattggt gcaattcttc agcttataca acgaactgct ggcagtgaaa cgtgcaacga
     4681 tccctccggc cgtgtaaccg ttcacagcac cctggttttc acaattttca ataagagatg
     4741 aagaaatgcc cgcaatacct cctgccatgg caatattgtt aatctcaatc tcttccgttt
     4801 cctcaacggg gtcatcggtc atgggaacag tcactacccc cgcattcagg caatcattga
     4861 tttttccgta acttacagcc actatgccgc cggctcgtct ggccgaagaa acaggagcgt
     4921 gattttcaca gtcggacaaa acggttccgg tatattgaac ggtacctgaa acacccgtga
     4981 tacctcctgc acttcctgca gaagcagata ccaccccgga ctcctggttg gtacagaaac
     5041 taacagtaac agtgccggta atataaccga taatccctcc tgctccggaa gaagcggtga
     5101 ccgttccgct gtttgttaaa aaagagagtg tataacctgt cccgtcagcc gtaccgattg
     5161 ttccgccaat gaaaattacc ccttcaacag gggccgtgtt ggcgcaatct ttaacgacat
     5221 tattggagaa tccagcaatg ccacctatag aagacgggcc cttaactgta cctgaattct
     5281 gcgaggtggt gatctctgaa taaaaatttc ctcccgtgat gcctccaaaa ccggtgttct
     5341 tgttttcatc ggatccggaa gcggtaatgg agccggaatt cgaacatcca ttgatggaac
     5401 aatcgagata ctgggtcccg catattcctc ctgcttcaga aacagcggat aaacctacct
     5461 cattgacaca gttgtcaatc tggctggaac tggctgaata accgcatatt cctccaatgc
     5521 gtgacgttcc tctaactatc gcccccgaat gattgacact attgctgaca acggccaggc
     5581 tgtagagctc gccgcatatc cctccggtct tttccccggt tccttccacg ctgccccggt
     5641 tgtgcgaaga attgataacc gcacttgtcc aggattgacc gcatatacct cccaccgcca
     5701 gggatccttt gacccgggca tcgtcattgt tctcacaact tactatgctg cttccattcg
     5761 atctgcctgc tatgcctcct acaggcgata gtccctggat ggttccgccg ttggaacatt
     5821 ccgtgacaac cgcagccttc aggttctcat ctgcgcgcat ttcacccact atgcccccgg
     5881 tcgcggtaga tgtgctcagc ccgctaatct gtatggtgcc ctgaaagtgg caacccgtca
     5941 aggtccctcc cagcatggtg ccgcagatcc cgccggccga catgctctga aactgtccgg
     6001 gtaccagagt aatggaacaa gtatgatcca gtgtgaggtc ctgtactgtg gctttccgca
     6061 catacccgaa caatccggca gacctgctgg aattctgggt gatcctgaga ttgctgatcg
     6121 tatggttatt cccattgaaa acaccggtga tactgctgtt gtcttcactg tatatcatgg
     6181 cattggtatg aagccctatg ggaatccagt tgggtatgga agacaaatcc aggtctgata
     6241 gcaggtgaat ggtgctgtcg ctgtccatcc agagtctcag gtcggcgaag gcattcacat
     6301 catcgcggaa caacaacagg tcttccagaa ccgaaatgcc cagcggcctt tgtgttacct
     6361 ccaggatagc cagtgaatcg ttgctggttt tgaaagtcag cagggctgtc ctggcctggg
     6421 tgagctgtga gggctcaaca ctgaatgtca tccggtattc tccccgattg cccgatgatt
     6481 gttcggatac aaggttgcac caggcagcat tgctgctatc atatacccat gtcgtatcac
     6541 tgaccagggt gaaatcgtag tttcctcctt tgcacggtac ctccatggaa accggtgtcg
     6601 tgaaaagggt gtctgccagg tcaggatcgt ccggatcttc cggatcttcc atggcatctt
     6661 ggattacttt aatgacagac tgtatgctgg cgttgttggt ggatttgatg acgatatcct
     6721 ggctgcgttc ctgtgttgta gtatttgttt ccacattgaa actcagaaga accgtttttt
     6781 cggaagttcc cacagatgac aggttggttc tacaccaatc caggggtatg ttcacgttcc
     6841 atcccgagtt ggaggttatg gccacggtat caagtcctcc ttcatgggtg aaatgcaatt
     6901 cctggggaac gctgttaatg taatagacca tgtcttcctt tttacaaccg gaaaacgcca
     6961 gcattatggt caatatgaat attggaagga ataaattttt aaccataatg caaagatgag
     7021 caaaaccttc caaatgttgc tccatgcgaa taaaaaaatg taatttcgct gatatgaaaa
     7081 caagatggcg tattcttttg gtcgtcttat tatctgtcct gggtctggta gcggctatca
     7141 tgacgattct ttccatttat atgacacctt cccgcctgac caccctgatc aaccagtatg
     7201 caggacagta catggaagct tcggttacca tatcggacgc tgccatccat gtttggaaag
     7261 attttcccta catatccctg gaactggccg atggtgaaat tctggttccc gcccctgaca
     7321 ctacgggaaa atgggcagac tccctgcttg tttttaagaa aatggaagtg accctgcgcc
     7381 ctgccgccct gttacggcgc atggtcgtta tcccccgcct gcaccttgaa caggcttcca
     7441 ttcatttgtt cacagacctg gaagaccgtt caaactggga tatttttccc ggatcggggg
     7501 acactgaatc cccctcttac ggattatcct ttggcaggct cagcgtacgc gataccctgc
     7561 gctttaccta tcatgattac aaagacaccg tgtattatag cgttgacatc accggcatga
     7621 cagcccgttc tcctttcagg gggtgggcat tcacggcaga catacaatcc tgctccaaca
     7681 cactgatcgt gggagagaac ctgctgtttg acaggattcc tttcaatgtc aaaggagata
     7741 ttgtactgga aaatgatttt gatgactact acttcaggaa ttgtgaaaca gttcttggaa
     7801 aagttcccct gtttatcaac ggattcatgg ggctgaggga agacagcctg gagctgcgcg
     7861 gcttatgcct gatagattcc accatgttga cagatcttta tgatgctttt cctctaaacc
     7921 tcctaccccg ggaaaaacca tttgcatctt ccatgcccat aaaggacctt gagctgttcc
     7981 tcaacggcgc ctataaatac gaaagtggtg aattccctgt atactgggct catcttgagg
     8041 ccggtccggg aagccttgtg catcttcctt cgggtatcat gataccggaa atcaagacca
     8101 ggatagcctc ttcctacgat cctggagaaa acaaccacgg aatacttgaa atggaggacc
     8161 tgacagtcag aagccctgcc cttgacttgt ccgtgaacgg gatactgtct tctgtcttta
     8221 aagaaccatc cgtcagattg tcggccggaa tcgaagctcg ccttgaccgc ctgcacgagc
     8281 ttgtggaggt ggtggaagaa ggccgggccg ttggaggtgt cacagccaat ctggaggccg
     8341 atttcagatt caaagacctg aatccggccg cgctgggcaa tgtgatgctc tcatcagatt
     8401 tccagacaga cggtttcacc ctggatttgc ctgcatacag tctgttttgc agggccgact
     8461 ccacccggct gcgtctgggt attcacccgg ggacctcttt ggatctggaa cttgtcagtg
     8521 acagtctgca aatcctttac aaggggcgtg aattggcagc cctctcagga actgagctta
     8581 aagccggcag caccccgggc atgttcacca tggacagcac taaggtccaa ccattgaacg
     8641 gatctttaaa aagcaggaac ctgcgggtat atgtaaatga ttccactcgc atcgggatgg
     8701 atggtggaaa tatcgtcttt tcagtaaagt ccgacagccg cgaccagagc cttccggtaa
     8761 tcgggctaac gctggaatcc cgttcagtcg gggccatgat gggcattcac cggggaagta
     8821 tctcggacct gcacctgcag gcagacctga cccgtaatgt caggacacag cacagagata
     8881 cagccggcct gtctgcgcgc agggactcgt tcctgctccg cagagcacgg cctcgtcacg
     8941 aattctccta tgccgacctg tctcttgatc tggacaggga agagcgcgga ttgctgcgcc
     9001 ggtggaatgt gaacgggaca ttacaggccg gcggaataca acttattaca ccttttatac
     9061 cgctcaacac acaggtaaga gatgcacatc ttgtactgca aaatgaccgg ctagagatta
     9121 cccataccgt catcagggca ggacgatccg acttattgct taacgggtat atgaccgata
     9181 tgcgccaggt gttgctggga cggggacgcc tgggactcaa ttttgacctc cagagcgata
     9241 ccctggactg caatgagctg atcggggcaa tcaattccgg aatgtcctta atggagaaac
     9301 aagacagcct gcccggttcc acggaaatcc ccgcttatca gaacctgctg gtcgtacccg
     9361 ggaacgtgaa cctgcagctg gtcctggccg tatccaacgc ctattacctt catttacaga
     9421 tgaaaggcct gcacggaatg ttacttgccg cagaccggta cctgcaactg gacgaacttc
     9481 aggcctcttc taacgcagga gatattacca tagacgcttt gtatgcaacc ccgtccaggg
     9541 acagcctgca ggtgggcctg catatggaaa tgaaggatat ccttctggaa gaagttaccc
     9601 ggctctttcc ttccatagac accttgttcc cgatgatcag gtctttcagg gggcttgccg
     9661 atattcagtt ctctgccacc tcgcagctcg ataccgacat gaaccttgtg ctctccacct
     9721 tgcggggcgc ctgccgcatt gccggcaacg gcctggtgct tctggacggg gagacttttt
     9781 ccgagatttc ccgcaaactg atgttcagaa aaaaggccct caacctgata gacaacatcc
     9841 gcgtagaagc caccgtggcg gacaaccggg tagatgtgta tcctttcatc atggaaattg
     9901 accgttacag ggcggccatt gccggatccc ataacatgga cggcacattt cattaccata
     9961 tttccctgct caaatctccc gttccgttca gattcggaat tgatgtgctt ggggtcccgg
    10021 aagattataa aattgacctt ggacgcgccc gtttcactga ccagggaatc ccggcccttt
    10081 cctaccggat tgacagcatc cgcgtgaacc tgagagaaca gatcaagaga tatttcgaaa
    10141 actatagctt tgaataaaat gtacaccata gcattaattc cggcacgcta cgcatctgaa
    10201 cgtttccctg gaaaactgat gcagaaactg ggagacgaaa cggtcatcct gcgtacctgg
    10261 cgcgcgaccc tggagacggg cctcttcaga aaagtagtcg tggtaacaga cagtgaggaa
    10321 atctaccgtg aaatcgtcag ccacggagga gaagcccgca tgagcaccgg taagcacctt
    10381 tgcggcagcg accggattgc cgaggcagcc gagcattacc cgcaggccga tgtgatcatc
    10441 aacgttcagg gcgatgaacc ctttacctcg gcaggcccgc tgaaagccct tctcggcgtt
    10501 tttgaagaag cgggcgctga atctgttgat gttgcttccc tgatgtctcc cttgggcgag
    10561 acggaggaac cggacaatcc caacgtggtc aaggtggtga cagacagaca gggcttcgcc
    10621 ctgcttttct ccagggcacc catccctttc acctgggatc ctgccgggta taaagccaag
    10681 actttcaagc acatcgggat ttatgccttc cgcagggagg ctttgctgcg tttcgcttcc
    10741 ctggcacccg gtcccctgga attgtcagaa aaactggaga acctgaggtt tcttgaatac
    10801 aatatgaggg ttaaaatggt agaagtaccc ggttattcca agggtataga tgttcccaaa
    10861 gatctggaag aggccaaaaa aatgattagt ccctgctcag ggatacacgt ttgacttctt
    10921 ccacccccag taccgattcc agcctcttga tgatattgta gatctctttg gcagagcgga
    10981 cttcaaggta tatatcacat tcaaaaaggg catcccttgt ggttacgctc atgttgttta
    11041 tgttcagtcc ccactcttcc gatatgatct gggatatttc aaaagtgatg cccttgcggt
    11101 ctaccccgaa tgcatggatg tgagccggat agtaagtgtt ttcatcggct tcaatctcga
    11161 catttacaat cgtttcccca tgttctgtgg ataatttgat agccctctcg caatcacggg
    11221 tgtgaacgaa gacttttccg cttccgtctt gggcttcaaa gccaagcagt tcatctcccg
    11281 tgaggggaga gcaacacttg gcaaatttgt atctggaagg ctttatggat atgttctgtt
    11341 tacggagggc acttcggatg caggattttg ccttatacgt aaccaccgcc tccagccagt
    11401 ctttttgagg catgatagcc gaggaggtgc caatttccac cctgtctccg cgtttgagta
    11461 cggtctttat ggagcagagc attccgttta cccgggcaaa aagggcgtga tctccgatct
    11521 cggagtgaat cacataggca aaatccagcg ccgaggcatt gctgggcagc agaatctgtt
    11581 ctccttttgg tgtgaaaacc ctgatatcgt cattatagag atttgctttc accccctcaa
    11641 tgaaaaaacc gtccttgttg ttaaagccgt catggtagac catgtctttg agcgttcccc
    11701 ggaattcctc cacccagcca tcaacccctc ctatccctac attcttgtga cgcttgacta
    11761 tacaaccgtg gatggaagct tcctccatcc gccggctctt gatatggacc tcggcccagg
    11821 ttccgtgttc ggtcatgaac atacagtgaa gggcttcgta gccgttggct ttgggattgt
    11881 cgacgtagtt gtgaaaactg cccacacgtt ccaggtagag atccgttagc agcgaataga
    11941 tttgcagact ctggttcttt tccgaaacgt cacagggtcc ctctgttttt acgtcaaata
    12001 caatattaaa aacataaacg ctttccaggc ggcggaaagg tacctgtctt tgttgcatat
    12061 ggcgccaaat ggagtaaaca ctcctgatat cctgcgatat ccgggcattt atgccatgtt
    12121 cacgcagggt ttgttcaatg ggagtaacaa agcgggtcct tattccctgg gtttcaacag
    12181 cataatcatc cagtatcttc tgaatcctgg catattcttc gggcgaccgg aacctgaaac
    12241 ttaggttttc cagttctgtc tttactttat acaggcccag gcggttggcc agcggagcaa
    12301 agaaaaaatc ggtttctccc gtaattttca tttgtttatg caggggcatg tcggccagtg
    12361 tcctcatatt atggagcctg tctgccagct tgatgagcag ggcacggatg tcgtactgaa
    12421 tggtcatcaa catcttgcgt atgttgtcca cctgcatgga cagtttgtat tgctcctttt
    12481 tttcttttgt aagtccgtct accaggtaag caatgtcttc cccaaaaagt tccctgattt
    12541 cttcgacggt atgcggagtg tcctctacca catcgtgcag cagggaagcg atgatgggat
    12601 ttgttccaag gcctatctcc tcgttaacta tgcgagcaac ggctatagga tgcaatatat
    12661 agggttctcc tgcttttctg gtttgtctgc agtgtgcctg gaaggcgtat tcataggcct
    12721 gtctgatgcg ctccaggtcc tcgggcagcc tgtcaacggc agcattcttc aggaaaatgt
    12781 cacgctgttc ctcaaccaac tggctgtatt gcgcctcatt cttttttccc attctcaaaa
    12841 tatccataaa acaaatataa gcaaaattga gtatatttgt ctgtcaacta atacgattaa
    12901 ttatggcaca tcaactacct cccctccctt tttccgccaa cgatctgtct cctgccataa
    12961 gttccctaac cataggttat cattacggca aacatcacca ggcttatata aacaacctga
    13021 acaacctgat cgtgggaact ccttttgaaa actccggcct tgaaaccatt attgccaagg
    13081 ctcctgccgg cgctctgttc aacaacgccg cccaggtttt caatcacact ttttatttta
    13141 atcaattcaa agcttccgct actgcaaaaa aacgtcctga aaatgagttg ttgagagcta
    13201 ttgaaaaaga tttcggcaaa tttgaaaaat tcgtagagac cttttccagt gcagctgcca
    13261 cggtattcgg ttccggatgg gcctggctgg tgcttgatca ggaaggcaac ctggtcataa
    13321 cccaggaatc caacgcagga tgccccctgg caaggaaaca gaaacccctt atgacatgcg
    13381 atgtgtggga acatgcctat taccttgatt accagaacag gcggccggat tacatcaagg
    13441 ctttctggga cattctggac tggaatgtga tcgaaaaacg tttcggcgca taatcaaagg
    13501 agctgcgaaa gctcctccag ggcaattgca agatgcaata atgtggcatt ccagttaatg
    13561 gcaatttcat tggaagcaaa ggcttccacc acgtccaggt aacattcgtc gggcaggtcg
    13621 gaaggataaa cacatccgtc ctgcctggca ggatttggtc cccctaccag gaatccggga
    13681 agcggtcccg gaatatcatc cgtagcggcc agacgatggt gaggattttg cgggctttta
    13741 ctcccggttc ctgtcacata acaatacccg gtgggatttc ttcccagtag gtaatccatg
    13801 ttatggtaag cctgcataag ataatccctg tttcccgtct ggctgaaccc gtagagaaga
    13861 gagatggcgt tgctgcaaca gctttcagac aggcatcccc agtagaaatc acgtgccgag
    13921 ttgccgtaag atgaagcaaa tggcgctccg ccggccagtg ataccacgct gtcacagaat
    13981 gcgatcagtt gttctttcat tgccggggtt gccttgttgt gtgtcaataa agcaaaaacg
    14041 cccagtccgt agacatctct ccaggtgggg gccgtgaatt tttcaggggc atattccaat
    14101 gctttatcca ggtaagcttc gtttcctgtg gttagataca actcgcatga tgcccagaaa
    14161 aaatcatctt ttgccgaaag atccccatag gcacctgttt gtatggctgg agtgaattgc
    14221 tcattcatcc ggaactggtt gtataaggca tcgggatgct ccagagccca gcagaatgcg
    14281 gcttctgccg cttttaccat gacagctgct gtggaggaat gatcttcaat atccctgtag
    14341 atacgggcgg cctgtgccat ggaggcagca aaatccaacg tagcagtaac gctcttttgc
    14401 acaacataac gcggtttttt acatgcaccg ggcttaatga acgcctcgaa ttcgggagtg
    14461 gtcaatttat ggtatacccc gccgtcggca ggatcctgca tggtcaggct ccattgcaga
    14521 ttgtacatca cctcgtccag caaccgtctg tttagggcat agccagggta ttgttcatat
    14581 ccggcaagta tgagcccggt cgtataagaa gcatttacaa tgtatttgtt gtaatcaccg
    14641 gcatcgtacc atcctccggc tgaagcaatg atggtccctt ccggcctttc ctgcgatgca
    14701 gccgaggcat gcaccagaac cactgtatcc gggtgtcccg gggtggatcg ttgtaaaaaa
    14761 aacgcttcca gggcggccga tgccaggggg gccaggatgc tgtccccgat tctgaaagca
    14821 ggcgactcca tcccttgttc
//
LOCUS       DAOSOF010000038        14332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4256_length_14332_cov_5.910415, whole genome shotgun sequence.
ACCESSION   DAOSOF010000038 DAOSOF010000000
VERSION     DAOSOF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4256_length_14332_cov_5.910415"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..616)
                     /locus_tag="PLM68_03545"
     CDS             complement(137..616)
                     /locus_tag="PLM68_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOT54472.1"
                     /translation="MDFVEFKKQYQANKDLWDAAFRAIAGADKDLSTFTESGKKEIVK
                     GRCHMIVSEYTPKSADNIRLEGHQNFIDIQISTGTVLWGTSTVEDSKVIVPYDPATDN
                     AFFRSSKVKVIRQKASKPYIFIFFPKNLHIPAYAKSRLLYKKPLKKVVVKIENVMNP"
     gene            complement(766..1806)
                     /locus_tag="PLM68_03550"
     CDS             complement(766..1806)
                     /locus_tag="PLM68_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOT54473.1"
                     /translation="MNYSLDYIGHPHQLYGAVPYQYAQGKAKGVKAIAVKNGAGLEVN
                     ILEDRCLDISHLSYKGINLSYLSKCGIVGPEYYDKTGYEFLQSFFVGFLTTCGLRHIG
                     APCTADGESFGLHGSISGIPAEETTACVDETDGNKVIRVSGIMREARIFRENLILRRS
                     YEIPVGQNHFIIRNKIMNMGFVAEQFMFMLHMNFGYPLLSPDAMLVLPSAEIEPRDKD
                     ARAGLTSYESINEPVSRYREQVYFHRLKSKANGQTSVGLDNKTLRTGVILSYSTKDFP
                     YTIQWKQFAKGDYVLGIEPASCKMTGREKAQEEGTMDYLMPQKSADFDVRVDILDGER
                     AIDQFINDVNQL"
     gene            complement(1811..2890)
                     /locus_tag="PLM68_03555"
     CDS             complement(1811..2890)
                     /locus_tag="PLM68_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT54474.1"
                     /translation="MKKLVLLLLLLFVVQLSNGQQKAHILSLKDSSSFSFVLLPDVQN
                     YVKYDYNQPVLELLTAWIADNVNNLNIKAALCTGDLVDQNECLVPPFPRFGNQTSTQQ
                     WEFVSRAFNRLDNILPYIISTGNHDYGYTRAETPESHFPEHFTVERNNQFSNCLVAVY
                     NNRLGVPTLENAAYAFTEKHWGKILVIALEYGARDQVLQWAKELCGSEKFRDHKVIVL
                     LHSYMGSGDNAPLLGKDHYKMTPLNGGKDIWEKLLSQTDNICLLICGHYAEANESFAD
                     NVGFRTDKNKAGNDVFQMMFNTQALGRGLSGNGGDGWLRVLEFMPDGKTVHVITYSPL
                     FAFSPRTKHLAVDTAPYNSFSFIIE"
     gene            complement(2897..3562)
                     /locus_tag="PLM68_03560"
     CDS             complement(2897..3562)
                     /locus_tag="PLM68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOT54475.1"
                     /translation="MKRSDVNQYIDYAMKFMAENKFCLPPWAYWTPSDWEQMKDRCEE
                     IFENGLGWDITDFGSGDFINTGLTLVTLRNGNLVTPSKQYCEKIMLVRNSQVTPPHYH
                     VLKTEDIINRTGGLLCMKLWNSAPDGSLSEGLLEVKIDSITTTIRSGEKICLKPGQSI
                     CYIPYLYHTFWAEGGNCLVGEVSSVNDDKTDNRFLEERGRFSAIDEDAPILHYLCNEY
                     PQK"
     gene            complement(3559..5190)
                     /locus_tag="PLM68_03565"
     CDS             complement(3559..5190)
                     /locus_tag="PLM68_03565"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOT54476.1"
                     /translation="MKRVLLYMVLCLLTVCSCRPSAKHFSATVDVPVVPVLAFKRTNK
                     IAQLNLVNHGTMPYTVKSMRVMLKGTHNLSDITKVSVYQKDRELGFIVPETEKSRHSL
                     TVDLNLPVNTDSLSLDIHITLKDTIDLSNRIQLEKVTINTSEGIVTTALRSPAVLRVG
                     IALRQKMQDGVHTSRIPGLTTTTKGTLIALYDARWESERDLQGDIDICINRSTDGGKT
                     WSPMQKVLDMGNWGNLPERYNGVSDGCILTDEKTGQLYVAGLWMHGILDPENGKWVKN
                     LNEASTEWNHQWRNFGSQPGYDVKQTSQFLISKSEDDGLTWSEPVNITQQVKPESWWL
                     MAPAPGHGITLEDGTLVFPAEGRTETGLQISTIIYSKDEGANWHPGNPAYTNTNECMV
                     VQLSDGSLMLNMRERSNRGRLEGNGRAVTVTKNLGNTWTEHPTSRNALIEPACMASLH
                     KHTYVNQNGDKQDLLFFFNPDSKIARNNFTLKCSTNDGLTWPDVLWTLIDQNNGNGYS
                     CITSVDNDTIGILYEGSGADLIFMTIEIEEIIKRL"
     gene            complement(5194..6633)
                     /locus_tag="PLM68_03570"
     CDS             complement(5194..6633)
                     /locus_tag="PLM68_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54477.1"
                     /translation="MKKILYITTLLLFAFLPGCHGDLNVEQPSEFTSLSMWTSEADAA
                     SAVNGAFHQLRTTLATSLPVYGDYRSALYGGGMMSVTDYDKMAVNVLARDMEGTDWTS
                     FYTTINTCNLILKYSPTITFSQESKKNEIMANAYFIRAYCYFMIARVWGDAPLLTTGF
                     ESDRQDDMYPVRTPASEIYAQVESDLAEADKLMPATVKEKHKASPAAISMLMADYYLW
                     KAKMLGGGNAALESAKTAVNKVLNNASYGLEDDFAKVFGVENEDSKEIIFSINYERNE
                     YVGGYPSYYLIPEQYVENKEFINNPLPVGSHQQYVSITNEYEAFLGSDPDDKRIPVTF
                     QIFPDGTIRWRWINKFKGEWINETRFFSSDIIIYRFAEAILMKAEIENALSNTAGAVA
                     ELVKIEKRAYKTTSRYTANMSRQAIDNAIVDEILKEFVSEAKSWWTLVRMGQAFTRIE
                     SLKGRENEENILLWPISSSSINTNPNIKE"
     gene            complement(6665..9865)
                     /locus_tag="PLM68_03575"
     CDS             complement(6665..9865)
                     /locus_tag="PLM68_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54478.1"
                     /translation="MKRIIHYLLFAGALLFSVSVSAQTQEITGKVTDVSGQPLQGVTV
                     FLKGTSTYTVSNNDGTYSISANPSQIITFTAIGFVTREVTVATQKVIDIILEEEVFNV
                     EELLVIGYGSQTKRTVTSSIAKVSGDEIRNSTVTSATDALKGRVAGARIFSSNNTPGA
                     EPTILIRGGSSISGNNDPLILVDGIERDLSGINPNDIESMEVLKDAASSAIYGSRASN
                     GVLLVTTKKGNNNSAPRITFEADVAVESTERNMEYLGSEEAITLMRKRLIQGPHPNYA
                     YANNYAYSSGNLTSSKYSLRALTSGESVPDGYLSMDDPINPGTTLIFQDNSWINESFR
                     TAIWQNYYVGIDGGTETIKYLASVGYTNDGGVAVGTNFDRISARANLDVKISKKLTFK
                     GGFDFNKMNTNYYASQYQVITRGLMTPTTQKLYYDEGQYAGTPTPGYNASSPTPVFYA
                     YYNDNAQKESKVGVNGILEYNIVEGLKAVAQVSLFNQYLTGDTFMRANIFNGTRPSSS
                     TLTNNERQKVEVYASYNKSVKEHSFSALAGYSYQRYDYKYLRAAASDAASDKIPTLNA
                     GPTKTDATTIKNAEVMIGYFGRFTYDYKKKYLLMLTFREDGSSKFVQGQQWGFFPGAS
                     AGWVISEENFFKSLKAVNNLKIRASYGQTGNNYVGYYDALGRYAISTMYQGEASIVPS
                     TMPNKGLTWESSTQLDLGFDLGMFNNRVQVTADYFNKVTDNLITSKVLPNTSGFSSIL
                     TNLGKVRFYGFDLEINTRNVVRNDFSWSSKLVMSYVKNIVLELPDNGRDKNRIGGYSV
                     TMADGSKLEFGGTAEGEPLGRFYGYMTDYIIATQEQADNALYDSQSRGWDWTKKNYVT
                     GKSGTSIGKKAIGDYEWKDLNGDGIINGSDMYLLGNTIPTFTGGFGNTLTYKNFSLYI
                     FMDWAVGHSISNNLLQRQMCNFFANNTSLPTEIRKAWDPESGQDVSEATYARFGGNDS
                     DDLNKNYRPASNVFTTRGDYLCLRDVTLSYVLSNAALKKAMIENITFTLSGNNLHYFT
                     SVIGMSPETGTSDAYSTGFYTYPPIRRISFGLKVTF"
     gene            complement(9884..10813)
                     /locus_tag="PLM68_03580"
     CDS             complement(9884..10813)
                     /locus_tag="PLM68_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT54479.1"
                     /translation="MINTPKLTGLISASFTPMHPDGSIHYERIKDFADYVIASECVNG
                     VFICGTTGESASLTTAEREKIAETWISHAGGRLKIIVHVGGTSICQSEELARHAQDIG
                     ASAIASIAPFFFKPASIEDLVQYFRPIAAAANRLPFYYYNMPSISGVSLPVPEFLKRG
                     AQVIPTLRGVKFTHNNLMEMQQCVQLNNGEFDILHGFDEILITGLSIGVKGAVGSTYN
                     YIPQIYKGIMQAMQRNDIAEARRLQYESVKILDVVIRHGGGVRGGKALMKLAGIDCGQ
                     CRLPISPVSDEEMEDIKKELQNTTFFNITNNRI"
     gene            complement(10810..13113)
                     /locus_tag="PLM68_03585"
     CDS             complement(10810..13113)
                     /locus_tag="PLM68_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT54480.1"
                     /translation="MIRNSPGYDRLTESKVLSMNIIHEQLNDLTLLGIVDKALIFRNN
                     TDNSLLYYSELSDKQNIEVDLDQKTIRSYPLQNGLLLTDSLSKGSYLLTIENTFLKIE
                     RLPDFPIDISNTQISIIENNGVLFLGASVQDTLMVYSLNRNGAPVNQWEILTHTGLGS
                     DNGFIMSVQSNGTDNCLYVFIKGSGILCYNLRSGHWTRKEIDTQPIEQTSSAFGKGAA
                     HIIFPHNDGQAINLFNTVTDTFYEMRIQPAEGVFMGVQISNNKVSAVYYDESGQSLEL
                     RNLFVEKVKNGFSAIDIIVLILYFGVLVFIGYYFSKRQKNSDDYFRGGQRIPWWAAGL
                     SLFGTALSAITFMAIPAKSFATNWSYMLFNGGILMVAPIIMFVFIPFFRKLNITTAYE
                     YLEVRFNSLIRVICSLAFIVFQIGRMGIILLLPAIAINVVTGFDIFLCIILMGVFSII
                     YTRMGGIEAVVWTDALQVVILLGGALFAIFHIVAQIPGGWTETISIACSNGKMSFGST
                     VFDLSDSTMWTVLIATFFTNLTTYGTDQSMVQRYLTTSSQKAARKSIMTNALLVIPST
                     IIFFFIGTALFVYYKNNPAHVTPILDSNDAIFPLYIYTRLPAGIVGLLIAGIFSAAMS
                     TLSGSMNSAATAYVVDIRPKLMRKKPTADLKVARRATLIAGALSLCFAIFMATWNISS
                     LWDEFNKILGLILGSMGGLFLLGMVTRKANAPGALIGMAVSIAVQIVVANYTPVHLLL
                     YTTVGFITCFVVGYLASYFFKTNKTIV"
     gene            complement(13238..13960)
                     /locus_tag="PLM68_03590"
     CDS             complement(13238..13960)
                     /locus_tag="PLM68_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT54481.1"
                     /translation="MAECKKIEDIGKVTLVDIVESSLINYIKQNHLKVDDYLPSETEL
                     AKSLGVARSVLREALSRLKMYGLIESRPRHGMVLREPSLFSPMKKVMQLNLWGEKTMY
                     DILGLRVTLELGMVDEIFSHINDKHIEELTRIVNVSTVLENNLYEGISEISFHSKLYE
                     ITGNSAFIMFQDLIHPVIGFIKSHFEENFKPINKQLEEKGLIVTHSDLLTYLKFRDKA
                     GFRKSLENHFMPYKIFLQERNG"
BASE COUNT         3749 a         3297 c         3096 g         4190 t
ORIGIN      
        1 tggctcctga agcaagcttg cttaattagt tgtacaattt attgaaaaaa tcacgacaat
       61 taatgcaaaa aaaaggaagt gaaaacatat ggccctcacg tacatggaat taccctgcta
      121 accaaaaaaa tatgaattaa ggattcataa cattttcaat ttttaccacg acctttttta
      181 gcggtttttt ataaagcaaa cggcttttag cataagccgg tatgtgaagg tttttgggaa
      241 aaaaaataaa aatgtatggc tttgatgctt tttgccttat cactttcacc ttgctggatc
      301 tgaaaaaagc attgtctgtt gcagggtcat acggtacaat aactttggag tcttccactg
      361 tacttgttcc ccagagcact gtgcctgtgc tgatttgtat atcaatgaaa ttttgatggc
      421 cttccaaacg gatattatct gcacttttgg gtgtatattc agacacgatc atatggcatc
      481 tgcccttaac aatctctttt ttaccacttt cagtgaacgt agacaagtct ttatctgctc
      541 cggctattgc cctgaaagcc gcatcccaca aatctttgtt tgcctgatat tgttttttga
      601 attccacaaa atccaccgag ggatgtatgt ccatttccag gccctgggga gcccaagccc
      661 cggatctgaa ccattgtctt gcttctcgta aaaggattct gtcggctttt tgtgcaaaga
      721 ggtcgttgaa ggagcccagc aggagggcaa aaaagagtat ccgtttcata attggttcac
      781 atcgttaatg aattgatcaa tggccctttc tccgtccagt atatctaccc tgacatcaaa
      841 atctgctgat ttttgaggca taagatagtc catggtgcct tcttcctgtg ccttttccct
      901 accagtcatt ttgcagctgg cgggctcaat tccaagcaca taatcccctt tggcaaactg
      961 cttccattgt atagtgtatg ggaaatcttt ggtgctataa cttaatataa cgcccgttct
     1021 caaggtttta ttatccaaac ccacgcttgt ctgaccatta gcctttgatt taagccggtg
     1081 gaagtacact tgctcccgat accgggaaac aggttcattt atggattcat acgaagtgag
     1141 accggcacgg gcgtccttat cccgaggttc aatttccgcg gaaggaagta caagcatggc
     1201 atctggacta agcaggggat atccgaaatt catgtgaagc ataaacatga attgttcagc
     1261 cacaaaaccc atgttcatga tcttgttcct gataataaaa tggttctggc ctaccggaat
     1321 ctcgtaggag cgacgtaata tgaggttttc cctgaaaatt ctggcttccc gcatgatccc
     1381 tgacacccgt atcactttat ttccatcggt ctcgtccaca caggctgtag tttcctcggc
     1441 gggaataccg gatatgctgc cgtgcagacc aaaagattcc ccgtctgccg tacagggagc
     1501 cccgatatgg cgaagaccgc aagtggtaag gaagcccaca aaaaaacttt gcaggaactc
     1561 atagcctgtc ttgtcgtaat attcggggcc tactattccg cattttgaaa gataggacaa
     1621 attgattccc ttgtaggaaa gatgggaaat atcaagacac ctgtcttcca gaatattcac
     1681 ctccagtcct gccccgtttt ttacggcaat tgctttcacc ccctttgcct tgccctgggc
     1741 gtactggtaa gggacagccc catacagctg atggggatgg ccaatgtaat caagtgagta
     1801 gttcatacaa tcattcaatg ataaatgaaa aactgttgta aggcgccgta tcaactgcca
     1861 gatgctttgt tcttggggaa aaagcgaaca ggggggaata cgtgattaca tgcactgttt
     1921 tcccatcggg cataaattcc aggacacgca gccatccatc ccccccgttc ccgctcaatc
     1981 ctctccccaa ggcctgggta ttgaacatca tctggaaaac atcgtttcct gccttgttct
     2041 tgtctgtacg gaatcccaca ttgtcggcaa agctttcgtt tgcctcggca taatgaccac
     2101 agataagcag acaaatattg tccgtttggg aaaggagctt ttcccagatg tcttttcccc
     2161 cgttaagcgg tgtcatttta taatggtctt tccccagaag cggggcattg tctccggatc
     2221 ccatatagga atgtaagagc actatgacct tgtgatctcg gaatttttcg ctcccgcaca
     2281 attctttggc ccattgcaac acttggtcgc gggctccgta ttccagggca atcaccagta
     2341 ttttacccca atgtttctct gtaaaggcat aggcggcgtt ttcgagcgtg ggcactccca
     2401 accggttatt atacaccgcc accaggcaat ttgaaaattg gttatttctt tctacggtga
     2461 aatgctcagg aaaatgggat tcaggggtct cggcccgtgt atagccgtag tcgtggttgc
     2521 ctgtactgat aatgtaaggg aggatgttat ctaggcgatt gaaagcacgt gaaacaaatt
     2581 cccattgctg tgtactggtc tggttgccga atcggggaaa cggcggaacc aggcattcat
     2641 tttgatctac cagatctccg gtgcataacg cggctttgat gttcaggttg tttacattgt
     2701 ctgcaatcca ggcggttaaa agttcaagta ccggctggtt ataatcgtat ttgacataat
     2761 tctgcacatc gggcaaaagc acaaaggaaa acgaggagct gtccttcagg gacaggatgt
     2821 gcgctttttg ttgtccattg cttaattgaa caacgaataa caacagtagc aataagacca
     2881 gttttttcat aacaatttat ttctgggggt attcattaca aagataatgc agaatgggcg
     2941 cgtcttcatc aatggcggaa aagcggcccc tttcctccag aaagcggtta tccgtcttat
     3001 catcattcac agaagacact tctcctacca gacaattccc tccctctgcc cagaaggtat
     3061 ggtataagta aggtatataa caaatactct gtcccggttt gaggcatatt ttttctccgg
     3121 atcggattgt ggtagtaata ctgtctattt tcacttccag taatccttcc gacaagctgc
     3181 catccggtgc actattccac agcttcatgc aaagaagacc tcctgtgcgg ttgatgatgt
     3241 cttccgtctt taggacatga taatgcgggg gagtaacctg actattgcgc accagcatga
     3301 ttttctcaca gtactgcttg gagggcgtga ccagattccc attacgcaag gttaccaggg
     3361 tcaaacctgt attaataaag tccccggatc caaaatcggt tatgtcccat cccagtccat
     3421 tttcaaatat ttcttcacac cgatccttca tttgctccca gtcggaaggt gtccaataag
     3481 cccagggggg aaggcagaac ttgttttcgg ccatgaattt catggcataa tcaatgtatt
     3541 ggttcacatc acttcttttc atagcctttt tatgatttct tcaatttcta tagtcatgaa
     3601 tatcaaatct gcaccgcttc cctcgtacaa tatgcctatc gtgtcattat ccacactggt
     3661 aatacaggaa tagccgttcc cattgttctg atctatcagg gtccacaata catctggcca
     3721 tgtcaggccg tcattggtac tacatttcag ggtgaaattg tttctggcaa tttttgaatc
     3781 cggattgaaa aaaaacaaca gatcctgttt atccccgttt tgattgacat aggtgtgttt
     3841 gtggagggat gccatgcagg caggttcaat cagggcattg cgagaggtgg gatgttctgt
     3901 ccatgtattg cccaggtttt ttgtcacggt aactgcacga ccgttgcctt ccaggcggcc
     3961 acggttgctg cgctcgcgca tattgagcat gagactgcca tcggacagct ggaccaccat
     4021 gcattcattc gtattggtat aggccggatt ccccgggtgc caattggcac cttcatcctt
     4081 gctataaatg atggtagata tctgcaatcc ggtttccgtg cgaccttctg ccggaaaaac
     4141 aagcgttccg tcttccagtg tgatcccatg gccaggagca ggtgccataa gccaccagct
     4201 ttcgggtttt acctgttgtg taatgttgac cggctcgctc caggtaagcc cgtcatcctc
     4261 actttttgat ataaggaact gcgaggtctg cttaacatca tacccgggtt gtgaaccaaa
     4321 attgcgccat tggtggttcc attcagtcga agcctcatta aggttcttca cccatttccc
     4381 gttttccgga tccagaatac cgtgcatcca caggccagct acataaagtt gacctgtttt
     4441 ttcatctgtt aggatacaac cgtcgcttac cccgttataa cgttcgggca gattgcccca
     4501 gttgcccata tccagtactt tctgcatggg actccaggtt tttccgccat ctgtgcttcg
     4561 gttaatacag atatcaatat ctccctgaag atccctttca ctttcccaac gggcatcata
     4621 aagggcaatc agagttccct ttgtggttgt ggtgagtccc ggaatacggg aggtatgcac
     4681 cccatcctgc attttctgac gcaatgcaat accaacgcgt aatacagccg gtgaccggag
     4741 agctgtggta actattcctt cagatgtatt gatggtgacc ttttccagct gaatcctgtt
     4801 tgataaatca atggtgtctt tcaaggttat atggatgtct aggctcagcg aatcggtatt
     4861 aaccggcaga ttcaggtcta ccgttaaaga atgcctgctt ttttctgttt ccggaacaat
     4921 aaacccaagt tcccggtctt tctgataaac cgagactttg gtgatatcgc tcagattatg
     4981 ggtgccctta agcattaccc tcatggactt gactgtatag ggcatggtcc cgtgattcac
     5041 aagattcagc tgtgctatct tgttggttcg tttgaatgcc agtaccggaa ccacaggaac
     5101 atcaaccgtt gcagaaaaat gtttggccga cggcctgcag gaacagaccg tcagcaaaca
     5161 taaaaccata tagagtaaaa ctcttttcat aaattattcc ttaatgttgg gattagtgtt
     5221 aatggaagag gaagatatgg gccacagtaa aatattctct tcattttcac gtcccttcag
     5281 ggattctatc cgggtgaaag cctgtcccat acgcaccagt gtccaccaac tcttggcttc
     5341 agagacaaat tcttttagta tctcgtccac aatggcatta tcaatggctt gtctgctcat
     5401 attggcagta taccgggatg tcgttttgta tgcgcgtttc tctatcttga ccagctcggc
     5461 cactgcaccg gctgtgttgg aaagggcatt ctcaatttcg gctttcatta ggatcgcttc
     5521 ggcaaaacga tatattatga tatcagacga gaaaaaacgc gtttcgttaa tccattctcc
     5581 tttaaattta ttgatccaac gccagcgaat ggttccgtca gggaatatct ggaaggtaac
     5641 cgggattctt ttgtcatccg gatcggagcc cagaaaagcc tcatattcat ttgtgatgga
     5701 tacatattgc tggtgactgc ctacaggaag cggattgtta atgaattctt tattttctac
     5761 atactgttcc ggaatcaggt aataggaagg atatccaccc acatattcat tacgttcata
     5821 gttgatagag aaaattatct ctttgctgtc ctcgttctca actccgaaaa ccttggcaaa
     5881 atcatcttca aggccgtaac tggcattgtt cagcactttg ttgactgcag tttttgcgct
     5941 ttcaagtgca gcatttcctc cccccagcat cttggctttc cacagataat aatctgccat
     6001 gagcatgctg attgcggcag gagaagcttt atgtttctct ttcacggtgg caggcattaa
     6061 cttgtcggct tctgccaggt cactttcaac ttgtgcatag atctcagatg cgggggtgcg
     6121 gaccggatac atatcatctt gtctgtccga ctcaaagcct gtggtcaaca ggggagcatc
     6181 accccatacc cgggcaatca taaagtaaca gtaagccctg atgaaataag cattcgccat
     6241 gatttcattc tttttgcttt cctgtgaaaa agtaatcgtg ggggaatatt taagaatcag
     6301 gttgcaggtg tttatggtag tatagaaact ggtccagtct gttccttcca tatcacgggc
     6361 aagcacattg acagccattt tgtcataatc tgtaacactc atcattcctc ccccgtacaa
     6421 tgcagacctg tagtccccat atacgggcag gctggtagcc agggttgttc tcagttggtg
     6481 aaaagcgcca tttacggcac tggcggcatc tgcttctgat gtccacatgg acaaagaggt
     6541 aaattcactt ggctgctcga cattcagatc accatgacaa cccggcagaa aagcgaacaa
     6601 caataatgta gttatgtata atatcttttt catacgattt gtatattttt tgttctccat
     6661 agttctagaa agttaccttc agtccaaatg atatacggcg gatgggtgga taggtgtaaa
     6721 atccggtgct gtaagcatcg gatgtacctg tttccggaga cattcctata acggaggtga
     6781 aataatgcag gttattcccg gacaaggtaa aggttatgtt ttcaatcatt gcctttttca
     6841 gtgcggcatt tgaaagtacg tatgacaggg tgacatcgcg caggcaaagg taatcgcccc
     6901 tggtcgtaaa gacattggaa gccggtctgt agtttttgtt cagatcgtcc gaatcgtttc
     6961 ctccaaaacg ggcatatgtg gcttcagaca cgtcttgtcc cgattcaggg tcccaggctt
     7021 ttcttatttc tgtgggcagg ctggtgttat tggcaaaaaa attacacatc tggcgttgca
     7081 gcaggttatt gcttatggaa tgacctacgg cccagtccat aaagatatac aacgaaaaat
     7141 tcttataggt taaggtgttc ccgaaacctc cggtaaaagt gggaatggta tttcccagaa
     7201 ggtacatgtc ggatccgttg ataatcccgt cgccgttaag gtctttccat tcatagtcgc
     7261 caatggcctt tttgccgata ctggtaccgc tcttaccggt aacatagttt ttctttgtcc
     7321 agtcccatcc acgtgactgt gagtcatata aggcattgtc tgcctgttcc tgggtagcaa
     7381 taatgtaatc tgtcatatac ccatagaaac gtcctaacgg ttctccttct gctgtccctc
     7441 cgaattcaag tttagatcca tcagccattg ttacggaata accgccaatg cggtttttgt
     7501 cccgtccgtt gtcaggtaac tcaagcacaa tatttttcac atagctcatc acgagtttgg
     7561 atgaccatga gaaatcattt ctgaccacat ttctggtgtt gatttccagg tcaaatccat
     7621 agaatcttac ttttcccagg ttggtaagaa tacttgagaa tcctgacgta ttggggagca
     7681 ccttgctggt aatcaggttg tcggtcacct tattgaaata gtcggctgtt acctggaccc
     7741 ggttgttgaa catgcccaga tcaaagccaa ggtccagctg ggtagaggat tcccaggtca
     7801 gccctttatt aggcatggtt gaaggaacaa tcgaggcttc tccctggtac atggtagaga
     7861 tggcataccg gcccagcgca tcatagtaac ccacataatt gtttccggtc tgtccgtaac
     7921 tcgccctgat tttcaaattg ttcacagcct taaggctttt gaagaaattt tcttccgaaa
     7981 tcacccagcc ggcagaagca cccggaaaaa aaccccattg ttgcccctgt acgaattttg
     8041 aggaaccgtc ttcacggaag gtaagcatta aaaggtattt cttcttgtaa tcataggtaa
     8101 aacgtccaaa gtaaccaatc atgacttcgg cgtttttaat ggtggtggca tctgtcttgg
     8161 taggtccggc atttagcgtc ggaattttgt ccgatgcggc gtctgaagcg gcagccctta
     8221 gatatttata atcgtacctt tggtatgaat agccggccaa agctgaaaat gaatgttctt
     8281 ttactgattt gttgtaggat gcatagactt ctactttttg tctttcatta ttagtcagag
     8341 tagaagaaga cggcctggtc ccgttgaaga tgttggcccg cataaaagta tcgccggtca
     8401 gatattggtt gaagagggat acctgcgcaa cggccttaag tccttcaaca atattgtatt
     8461 caaggattcc gttaacaccc actttgcttt ctttctgggc attatcgttg taataggcat
     8521 aaaagacagg agttggtgaa gatgcattgt atcccggggt aggagtaccg gcatactgtc
     8581 cttcatcgta atacagtttc tgtgtagtag gggtcataag acctcttgtg attacctggt
     8641 attggctcgc atagtaattg gtattcatct tattgaaatc gaaaccaccc ttgaacgtaa
     8701 gcttcttgct tatctttaca tccaggtttg ccctggcgct gatccggtcg aaattcgttc
     8761 ccacagccac gcctccgtca tttgtataac ctacgctggc aaggtattta atagtttcgg
     8821 tcccgccatc tatccccaca taatagttct gccagatagc ggttctgaat gattcattaa
     8881 tccagctgtt gtcctgaaaa ataagcgtag tccccggatt gatggggtca tccattgaca
     8941 ggtaaccatc gggaacactt tctcccgacg ttaaagccct gagagaatat ttggacgagg
     9001 tcaggtttcc cgagctgtaa gcgtagttgt tggcataggc ataatttggg tgagggccct
     9061 ggatcaaacg ttttctcatc agggtaatgg cttcttccga tcccagatat tccatgttgc
     9121 gttcagtgga ttctacagct acatctgcct caaaagttat cctgggcgcg ctgttattgt
     9181 tccccttttt tgtggtgaca aggagaacac cgtttgaagc cctggaaccg taaattgctg
     9241 acgaggcagc atctttcagc acttccattg attcaatgtc attgggattt atacccgaca
     9301 gatcacgttc aattccgtca acaagaatga gcgggtcatt atttcccgag atggaagatc
     9361 cccccctgat gagaatggtg ggttcagctc caggagtgtt gttggaggag aatatccggg
     9421 ctccggccac tctccccttc aaagcatctg tggctgaagt taccgttgaa ttacggattt
     9481 catcccccga cacctttgct atagacgaag taaccgttcg cttggtttga gagccataac
     9541 caatgaccag cagctcttct acattaaaca cttcctcctc caggataata tcgatcacct
     9601 tctgcgtggc cacagtaact tccctggtaa cgaagccaat agcagtaaaa gtaatgatct
     9661 gtgaaggatt ggctgaaatg gaatacgtac cgtcgttgtt gctgacagta taggttgaag
     9721 tcccttttag aaaaacagta accccttgaa gaggctgccc tgaaacgtcc gtcactttcc
     9781 cggttatttc ttgagtctgc gcactgacac tgactgaaaa aagcaacgca cctgcaaaga
     9841 gtaaatagtg gataattcgt ttcatacaaa tgattattat tagttaaatc ctgttgtttg
     9901 ttatgttaaa gaatgtggtg ttttgcaatt ctttttttat gtcttccatt tcctcgtcgg
     9961 aaacaggtga gatgggcagg cggcattggc cacagtcaat tccggccagt ttcataagtg
    10021 ctttccctcc ccgtacacca cctccatgtc tgataactac atccagtatc tttaccgatt
    10081 catactgtag tctgcgagct tcggcaatgt catttctctg catggcctgc atgattccct
    10141 tatagatctg gggaatgtaa ttataagtgc tgcctacagc accctttacc cctatagaga
    10201 gaccggtaat tagtatctca tcaaatccat ggagtatatc aaattcgcca ttgttcagct
    10261 gtacgcattg ctgcatttcc ataaggttat tatgggtgaa cttaacacca cgcagggtgg
    10321 gtattacctg ggccccgcgt ttgagaaatt ccgggacagg cagggaaacg ccagaaatgg
    10381 aaggcatatt gtaataataa aatggcagac ggtttgctgc ggctgcaatc ggccggaaat
    10441 attgaaccag atcttctatg ctggccggtt taaaaaagaa cggtgcaatg gaggctatgg
    10501 cactggctcc aatatcctgt gcatgtctgg ccagttcttc cgactggcat atggaagttc
    10561 ctccaacatg tacaataatt ttcagcctgc ctccggcatg actgatccag gtttctgcga
    10621 tcttttccct ttctgcagtg gtaagtgaag ccgattcacc ggttgtgccg catataaaaa
    10681 cgccatttac gcactcagag gcaataacat aatctgcgaa atccttaatt ctttcatagt
    10741 gaatgctccc gtcggggtgc ataggggtaa acgaagccga gatcaacccg gtaagttttg
    10801 gtgtgttaat catacaatag tcttatttgt tttaaagaaa taacttgcca gatagccaac
    10861 cacaaagcat gtaatgaagc ctacagtggt ataaagaagc aggtgtaccg gggtatagtt
    10921 ggcaacaaca atctgtacgg caatgctcac tgccatgcca atcaaagccc cgggggcatt
    10981 tgctttcctg gttaccatgc ccaataagaa cagtccgccc atgcttccca aaatgagccc
    11041 taaaatttta ttgaactcat cccacaaaga gcttatgttc caggtagcca taaaaatcgc
    11101 aaagcacaaa ctcagcgctc ctgctataag tgtggccctg cgggccactt tcagatcggc
    11161 tgtcggtttt tttctcatga gttttgggcg gatatccacc acataggcag tagctgcaga
    11221 attcatactc ccgctcaatg tggacatagc ggcagaaaaa atacctgcaa tgagcagtcc
    11281 cacgatccct gccggcaacc tggtatagat atacaatggg aaaatggcat cgttactgtc
    11341 cagtatcggg gtgacgtgag cgggattgtt tttgtaatac acaaataacg ccgtcccaat
    11401 aaagaagaaa attattgtag acggtatgac caaaagggca tttgtcatga tgcttttcct
    11461 ggcggctttt tgtgacgacg tcgtcaggta tcgctgtacc atactctgat ctgtgccgta
    11521 tgtggtgaga ttggtaaaga aagtggctat gagtacggtc cacatagtag agtcggaaag
    11581 atcaaagaca gtgcttccaa agctcatctt gccatttgaa caggcaatag aaattgtttc
    11641 agtccatccc cccggaattt gtgcgactat gtggaaaatc gcaaaaagtg cccctcccag
    11701 aagaatgacc acctgcagtg catcggtcca gacaacagcc tcgataccac ccatgcgcgt
    11761 ataaattatg ctaaaaaccc ccattaggat gatacagaga aatatgtcaa aaccagtaac
    11821 cacattaatg gctatagcag gcaataaaag aatgattccc attctgccta tctgaaacac
    11881 aataaaagcc agactgcata ttactctgat aaggctgttg aaccggacct caagatattc
    11941 gtatgcagtg gtaatattta atttccggaa gaaagggatg aatacaaaca taatgatggg
    12001 agcgaccatc agaatacccc cgttaaagag catataactc cagttggtgg caaaagattt
    12061 ggctggtata gccatgaacg ttatagcact caaagccgta ccaaaaagac tcagacctgc
    12121 agcccaccat gggatccttt gtccgccccg gaaataatca tctgaatttt tctgcctttt
    12181 ggaaaaatag taaccaataa agaccaggac tccaaaatag agtataagaa caatgatgtc
    12241 aatggcagaa aaaccatttt tcaccttctc cacaaataaa ttcctcagtt ctaaagactg
    12301 gcctgactca tcataataaa cggccgatac cttgttgttt gaaatctgaa ctcccatgaa
    12361 cacaccctct gcaggttgga tccgcatttc ataaaaagta tctgtcactg tattgaacag
    12421 atttatggcc tgcccgtcat tatgtgggaa aataatatga gccgctccct ttccaaaagc
    12481 ggaggaagtc tgctctattg gctgtgtatc aatctctttt ctggtccaat gacctgatct
    12541 taagttataa cacaaaatac cgcttccctt tataaataca tagaggcaat tgtctgtacc
    12601 attggattga acagacataa tgaagccgtt atctgaaccg aggcctgtat gtgttaagat
    12661 ttcccattgg ttaaccggcg cgccattcct gttcagacta taaaccatca gggtgtcctg
    12721 taccgatgcc ccaaggaata atactccatt attttcgatg atggagattt gggtattgct
    12781 aatgtctata ggaaaatccg gcagtctttc aattttcaga aatgtattct ctattgtgag
    12841 cagataagat cccttactta aggagtcggt cagcaacaga ccattttgaa gaggatagct
    12901 cctgattgtt ttctgatcaa gatccacctc aatattttgt ttatctgaca gttcgctata
    12961 ataaagcaat gaattgtctg tattgttgcg gaaaatcagg gccttatcaa cgattcccag
    13021 aagggtaagg tcattaagct gttcatgaat aatattcatg ctcagcactt tgctttctgt
    13081 caggcggtca tatccgggtg agttccggat catagcagta ttatttccag agcaggcagg
    13141 taacagcagc caaaggctta caatgctcac tattacaatt atcctgtttt gtttggtcat
    13201 atttgtctta tttgtctgac aattgttttg aaaaaaatta accgtttctt tcttgcagga
    13261 atattttata cggcataaag tgattttcca gtgacttcct gaatcctgcc ttatcccgga
    13321 acttaagata agtaagcaga tctgagtggg tcactatcag acctttttct tccaattgct
    13381 tgtttatggg tttaaaattc tcttcaaaat ggcttttaat aaaaccaatt accgggtgaa
    13441 tcagatcctg gaacataata aacgccgagt tcccggtaat ttcatacagt ttggagtgaa
    13501 aactaatttc gcttattcct tcatacaaat tgttctccag cactgtgctt acatttacta
    13561 tcctggtgag ctcttctata tgcttatcgt ttatatggct aaaaatttca tccaccatgc
    13621 caagctctaa agtgacccgg agccccagaa tatcgtacat tgttttctct ccccacaggt
    13681 tcagctgcat cacttttttc atgggggaga agagggacgg ttcgcgcaga accattccgt
    13741 gacggggacg cgactcaatc aatccgtaca tctttaagcg gcttagcgct tcacgtaata
    13801 cacttcttgc aacacccaga cttttggcca gttccgtttc gcttggaagg taatcgtcta
    13861 ctttaagatg attctgcttg atatagttaa tcaaagaact ttctacgata tcaacaaggg
    13921 tgactttacc aatgtcttct atttttttgc actctgccat aagctggatt ttcgatttgt
    13981 ctgattaatc atatcaattg taaagataca aatattttca tatatgcaaa ttttttttca
    14041 tttttttgga tcccggcctg acttatctta gaatccactc atgtccaatg cattcaaaag
    14101 cttgcagcga ctgcatattt tccttctgtt taaaaccata ccggactctg gctttgcatc
    14161 tatgaaaccg gagaaatttt tgaccaagcc ggggcgccaa ctcttccgtt gttaccacaa
    14221 acgttcgcag acccattaag aaccgggtgc ttttttggaa cgccgagact tcaacctttc
    14281 ctgcaggcgg tcgtctgagt gccaagaacg gagtgctttt tcttttaagc cg
//
LOCUS       DAOSOF010000039        14299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4269_length_14299_cov_5.475218, whole genome shotgun sequence.
ACCESSION   DAOSOF010000039 DAOSOF010000000
VERSION     DAOSOF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_14299_cov_5.475218"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..781
                     /locus_tag="PLM68_03595"
     CDS             104..781
                     /locus_tag="PLM68_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54482.1"
                     /translation="MISQIILTVIILVAIVLVLVFTELSYRRLGLPTEITRKFAHFTS
                     TLSTILFPFIFTSHWSVLVLAVFFFLILFFSRHGSKLNSIHKIERKSVGSFILPVAIY
                     VTFLVSWLLREKFFYILPILILGICDPMASILGMNLKRNNRHIRIFGHTMKKTVLGTL
                     SFLICSFLISVIALYIYMGVFNFKTFWLALAVSVTATVTELFAGKGWDNLFIPLSVQL
                     VLIFFLS"
     gene            804..2228
                     /gene="amrB"
                     /locus_tag="PLM68_03600"
     CDS             804..2228
                     /gene="amrB"
                     /locus_tag="PLM68_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT54483.1"
                     /translation="MKTTPRNPAVAGRFYPATPGRLQEELVRLFKQARKKECNNVRAV
                     ISPHAGYVFSGGVAASAFNQVDPQKKYKRIFVIGSSHHVRLQGAAVYTSGDFLTPLGV
                     VRVDTEFCRQLETDHPGLFLADTRAHRDEHSIEVQLPFLQHILGDELLIVPIIIGTSS
                     TVVCKRIADVLSPFLNEDNLFVVSTDFSHYPPYDRAVDVDACTKDAILSNNPDTLISV
                     MQKNESENIPGLATCLCGWTSVLTLLYMTTGNDMMQYVAIDYKNSGDAPEYGEKEQVV
                     GYWGMAVCDNEMKFTRTEEARLLEIALEALKGAVTSGKTAKLNSPGKYSDALQRHCGA
                     FVTLHQRERLRGCIGRIQSDIPLYLLVSEMAVAAGLYDDRFPPVTKKDLEEITVEITV
                     LSPLEEIQDIHRIVPGKHGILIQGRGRSGVFLPQVATETGWTKEEFLGHCSRDKAGLG
                     WEGWKTARIFIFTGTIITHHTPGT"
     gene            complement(2207..3220)
                     /gene="amrS"
                     /locus_tag="PLM68_03605"
     CDS             complement(2207..3220)
                     /gene="amrS"
                     /locus_tag="PLM68_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT54484.1"
                     /translation="MFYKVGENRWRTMAGTTVKCLLCPHECLIRDGETGVCHTRTNKE
                     GTLYSVAYGNPCSVAVDPVEKKPLYHFLPGTEILSIATAGCNFHCLNCQNSSISQVSP
                     LSYDSYDMLPDQVAEMAVNCHVPSIAFTYTEPTVFYEYMYDSAVVAREKGLKTVAVSN
                     GYINPEPLATLIPYLDAANIDLKCFDDKTYRRLTGGRLQPVLDTLLTLKKAGVWIEIT
                     HLVIPGLTDDPAKFKSMCRWLAGNGFETVPLHISRFFPAYKLKDVAHTPIESVIHARN
                     IALESGLKFVYPGNVATGKDSHTRCPGCGRVVAERDQYTLVSLRLDKGSCNYCGTYVP
                     GVW"
     gene            complement(3679..7086)
                     /locus_tag="PLM68_03610"
     CDS             complement(3679..7086)
                     /locus_tag="PLM68_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT54485.1"
                     /translation="MKQFDALQVPLEGSNLIEASAGTGKTYSIAVLALRMIIEKGLTV
                     KQILMVTFTNAAVAELELRIRLFVRQAYGYACHRDDRVDVSIKKVVDGTGQTDRPDKE
                     TVRERLATAVRDLDDTQIMTIHGFCQQTLLEFAFETEQAFDAEVLSDQTLLNERVCNR
                     FWREEVSVLDPVLLKLLLYKKGKFPFKKSILTLFVDKVLSGKKLITAPLEGPGDFSTV
                     VLEDKHAMPGKMSGMEVTGKELSDPEVLEEIRLSLKKTEEAYRLFCQKAESQFPGMRT
                     QDQTNAHARKLLEQSATAQDFIDLFEARWVKKYLVACFPDIAELYEPYYKQQQEMLRF
                     RTSLVEHLLMKLQAVAEERMREVKGRKNVVGFDDLINSLSKAVEKESVCRLLRDKYAA
                     VFIDEFQDTDREQYKIFHDVFSSPAVQEMHDHVVFYIGDPKQSIFGWRKADLNTYKTA
                     RGQVKSIYTMNSNFRSTSRLIDALNSFFSLENAFLDPEILYFPVRAGTKRAEMTEKGT
                     QSVPISICNYDIVENIPDMVCSKVVEILSSDSYALPSRDGGREQIRPSDIGIIVRTNK
                     QAKIIRNELAHRNVPAVTLDDTRVLSSPQARQLHNVLLAIRNSGRVSLGRALLNPYFG
                     FDTEAVISMDEEKELERFREMHAIWSEWGIYNALSAFLKIYRVRDHCTAEGNLQGQRA
                     ITNYLHIMELLHKAEIRSSLSPDELISWLSRQITGDNKVEDEYQQRIESDENAVQITT
                     IHKCKGLTYKIVFAPYLNTIWKEREFIDFQLPYPRQEYVMAVNKTDEFVQMAKNQFVQ
                     EHRRLIYVTLTRAVYKCYVYTKDTSCSLQPFMVALGGGIKGLIEPLGHSDEKIPHTWA
                     GGRKKSVVEKPVSREPNDVPPMEGAWGVRSFSSLVGEMPFVLPVQEPVSPGLLQAGST
                     DVRSAYDDFIFDRLPRGAKTGVFIHHLFQHIVFDDSTGWPDAVDRAMRMFPHCCAVED
                     REHLLTMINHVLHVRIGEDLVLAQVNDREKAGEMAFFYNLQAEGLMMGFMDLFFRFRD
                     KYYILDWKSNYMGSTPEDYNQTAVDNVVRESRYDLQYNIYTMAALRYLSSKISGFDAS
                     RDFGGVVYLFVRGIREGFSHGVYLKKGAQVLGQPFFSEDFPSNLMRP"
     gene            complement(7083..10280)
                     /locus_tag="PLM68_03615"
     CDS             complement(7083..10280)
                     /locus_tag="PLM68_03615"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT54486.1"
                     /translation="MSFMAAVIYVSDQLEILCGKLAQQIDRMDYPSADADTIFLPEVL
                     ITQTAGMESWLSTELTRRNGVFANFRFMKQDQLLEEAYAGLYGKKRYTTNIRPAIYEL
                     LGSESFITRFPDIAAYYSQDKQNNELRRMQLAGKTSDLFDQYQVYRPDMMSAWESAPD
                     RRSYPPDGDQESSPGDPQDSDHEAWQRWLWNSVREKYPWFCPKHRMQARLLELLDEPQ
                     NWPKIRQMLPRLSLFGLSIFTTFHNRVLEKLARIIDVAYYLVLPSEHFFEKGNFSNPL
                     SASLGRKYAEMAAMLPRECEVVPLWGQHSGETLLGKFRNTLRRDLVPQPGTLTEKDTG
                     DGTLVINACYTPVREVEVFYNYLLSLMHAGAGLKPEDVLVLLPDVDTYAPYVKAVFGN
                     GPYPIPFFIAGESQDQQDSIAGALMALMDFDALDGFTAEEVVSLLETKRICQKYGITD
                     MQYLRDAIEALNIRFGLENRVEDDTRFVSWQYGFEKIMMGFAILTEQSIPSSQGSYTL
                     FPWREAETSVAYDLLRLKAFLDDLTIYARTKTEHRSLADWKTFLLEEMLERSVAYSGQ
                     DDMELDHIYRRMEILERSYETTPLSFKVFRHLVADTLLTDTRSGMYITGNVNFLPVIP
                     GRGIPKRVIAILGLNGNVFPRKDSSLGFDLLQKEPRLGDRSKKDNDKLIFLESLVASR
                     DYLYISYIGRDIQDNTVLPPSIVLDELTDYLESLVPEESRELVRCGVSQVHPLHGFSS
                     RYDGKDERLFTYLFSDLPDQSGGGKALQPDIAGDTIPGTVQLEDLAQFFTHPLEWYYQ
                     KILGIRYDEPKQPLPESEIFDLDNLQYWELKRFLVQTDPVGEQELVAAWTDKTMKEGN
                     MPLARIKEMKVSEAQLEIGDLQASWQELTAGKTGKSCQVEITVGGIVVRGAVTNLFDN
                     SLYDFTVSKDALKHQVKTRIRHLVLCACGFSGQAVFLDLHGKKFHIPAIAQHQAMEQL
                     SRLTALFRQGCEKRLPITLKAANAFFLSGGDSGEPLNEALREIKKEAEGTRTTEPDPY
                     VALAWEEGVFADDRHEALEQLMNEYAALILTPWET"
     gene            complement(10277..11896)
                     /gene="recD"
                     /locus_tag="PLM68_03620"
     CDS             complement(10277..11896)
                     /gene="recD"
                     /locus_tag="PLM68_03620"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOT54487.1"
                     /translation="MDVYRIFSDYFNQFQPGLGILAGALLKRLDEGHMCLDLKTLDDD
                     HPEEALPAGILLPGPYVSHDPSALKPFIVYLGNLYFQRYFLYETNIEQKIRQLIAEGS
                     KLRNQRIRELEPLQPLIKELFKENAEAFRPALLGNFCIITGGPGAGKTTLAREVLRVL
                     YARQPDLKVALAAPTGKAAARINESMTVLRQQIPGLPMASTIHRLLGSVNGSIFFRHD
                     EENPMNHDITIVDEASMIDGALMAKLLNATSPEKRLILLGDKDQLASIEAGSVFGDLC
                     KTEGTGSVLDGHVIRLTKSHRFDADKGIGLFSRSVLAGDSDCIVRTMDHPDDALSIDT
                     TYDDKLFHEFAVMYRDYITEPDIATALEKIGKIRVLCAIREGEHGVYQTNRRIAGYLC
                     SLKLPIEPSAGFYHNQPVMVTSNMYALKVFNGDVGIIRRDPLDPGNRLYAWFPGPDGN
                     LMRILPGYLTHWETVFAMTINKSQGSEFENVLVLLPDNKDVRILTRELLYTAVTRGQK
                     RVLVQAPQDVLEETVRKRISRVSGLANRLKA"
     gene            complement(11903..12319)
                     /locus_tag="PLM68_03625"
     CDS             complement(11903..12319)
                     /locus_tag="PLM68_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT54488.1"
                     /translation="MKTYRQEFYVETPTRRAFVNITSRVQECLEESGIQEGLLLCNSM
                     HITSSVFINDDESGLHHDFEKWLERLAPELPHSQYRHNGLENNADAHLKRQIMGREVV
                     IAVTNGRLDFGTWEQIFYGEFDGKRKKRILVKIIGE"
     gene            12447..13253
                     /locus_tag="PLM68_03630"
     CDS             12447..13253
                     /locus_tag="PLM68_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT54489.1"
                     /translation="MMNPVPTNGYCLVTGAAHGLGRAFALELAGRHLNTLLVDLPGNG
                     LKELCSHIHEKYNVLSHCIECDLTKKEQVLDLGTSINRQFPVFMLINNVGTGGSVRFE
                     EADTDYLDRIIQLNVMATTLLTRQLIPNLLAQKKAYILNVSSMAAFSPMGYKTIYPAS
                     KVFVHYFSRSLYQEYKHTNLFVSVVNPGPMKTNPDVTRRIEKQGFFSHWGVMSPEKVA
                     AISIRQLFKQDTLIMLSLVNGLEWLALKLVPIYIRLPLLSRAMKNEINIV"
     gene            13254..14240
                     /locus_tag="PLM68_03635"
     CDS             13254..14240
                     /locus_tag="PLM68_03635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT54490.1"
                     /translation="MRTVLVTGANGLLGTNVVLELLSRGYAVKGLVRSRRRFMDYSHP
                     LLELVIGDIRDKTSLQAAARGCDFVVHTAADTSQKHLKVTDYDAVNLEGTKNILDLCR
                     QEGIRRLVYIGTANTCGFGSRENPGDEQSPPAPPFTGCLYALSKIKAGELIDTAVSEG
                     MDIVTISPTFMIGPYDYKPSSGRIINMALNKKVVFYPPGGKNFVHAADVARGVVNALE
                     KGVAGDNYLMANENLSYRDFFQIISRLTGSRPLMIRLPRPLLLILGLAGNFLRRLGAE
                     IPLCSNNMKILCINNFYTNQKALSRFNMSFLTTEEAVRDALRTGPFRAILKN"
BASE COUNT         3442 a         3646 c         3444 g         3767 t
ORIGIN      
        1 cacgccttat tcccggacac ggaggcgttc tggaccgttt tgacagcatg attgccgccg
       61 gtgcctggat ggccctgatg ggaacattaa taaactttac atcatgatca gtcagataat
      121 attaactgtc atcatcctgg tggccattgt cctggtcctg gtatttaccg agctttccta
      181 caggcgcctg gggttgccaa cagagattac ccggaagttc gcgcatttta cctctaccct
      241 gtccaccatt ctctttccgt tcatcttcac cagccactgg tctgtcctgg tgctggccgt
      301 tttctttttt ttaatcttgt tcttcagccg acacggttca aaactgaatt caatccataa
      361 gattgaacgc aaatcggtgg gaagctttat ccttcctgtc gccatttacg ttacattcct
      421 ggtatcctgg ctgctgcggg aaaagttctt ttatatcctg cccatactca tccttggcat
      481 ctgtgatccc atggccagta tactgggtat gaacctgaaa cgaaataaca ggcatatacg
      541 catttttggc cacaccatga aaaagactgt attgggaacc ctttccttcc tgatctgtag
      601 ttttcttatc tctgtcattg ccttgtatat ctatatgggt gttttcaatt tcaaaacatt
      661 ctggcttgca cttgctgtca gcgttacagc aaccgtaaca gaattgtttg ccggaaaagg
      721 ctgggataac ctgttcatac cgttaagtgt ccagctggta ttgatattct ttttatccta
      781 atgctttttc ctacctttga tccatgaaaa caacaccacg gaacccggcc gttgccggca
      841 ggttttatcc tgccacaccg ggaagattac aggaggaact ggtgcggtta ttcaagcagg
      901 ccaggaaaaa ggaatgcaac aacgtcaggg cggtcatcag tccgcatgca ggatacgttt
      961 tttccggagg ggttgcagcc tcggctttta accaggttga cccccaaaaa aaatacaaga
     1021 ggatctttgt gatcggatcc tcacatcatg tacgcctgca gggtgccgcc gtttacacca
     1081 gcggtgactt tctcacgccg ctgggcgttg tcagggtaga cacggagttt tgccgccagc
     1141 tggaaactga tcatccaggc ctgttcctgg ccgacacccg ggcacaccgg gatgaacata
     1201 gcattgaggt gcaattgccg ttcctgcaac acattctggg cgatgaactt ttgatcgttc
     1261 caataatcat aggcacttcc agtacggttg tatgcaaaag gatagcagat gtcttgtccc
     1321 cctttctgaa tgaagataat ctttttgtgg tgagcactga cttttctcat taccccccct
     1381 atgacagggc tgtagacgtt gacgcctgca caaaagacgc tatcctgagc aacaatcccg
     1441 acaccctgat aagtgtaatg caaaaaaatg aaagtgagaa cattccaggg ttggccacct
     1501 gcctgtgcgg gtggacctcg gttttgaccc tcctctacat gaccactggg aatgacatga
     1561 tgcaatatgt tgccatcgac tataaaaatt ccggagatgc acctgaatat ggagaaaaag
     1621 aacaggttgt gggttattgg ggaatggctg tttgtgacaa tgaaatgaaa ttcacccgga
     1681 cagaagaggc ccggttgctg gagattgccc tggaagctct gaaaggggct gtcacttcag
     1741 gaaaaacagc caagttgaat tcaccgggga agtattcaga tgccctgcaa cgccattgcg
     1801 gtgcgtttgt aacgctccac cagcgtgaac gccttcgcgg atgcatcggg cggatacagt
     1861 ccgatatccc cctgtacctc cttgtcagtg aaatggctgt tgccgcaggc ctttatgacg
     1921 accgttttcc gccggtcaca aaaaaggacc tggaggaaat caccgtggaa atcacggtat
     1981 tgtctccgct ggaagaaatt caggatatcc accggattgt gcctggcaaa cacgggatac
     2041 tgatacaagg ccggggccgc tcaggggtat tcctgcccca ggtggcaaca gagaccgggt
     2101 ggaccaagga agagttcctg ggtcactgct cccgtgataa agccggtctg ggatgggaag
     2161 gctggaaaac agcgcgtatt ttcattttta cagggaccat cattactcac cacacacccg
     2221 gcacataagt cccgcagtaa ttgcaggatc ccttgtcaag acggagtgat accagcgtgt
     2281 actgatcccg ttctgccacc accctgccgc aaccgggaca ccgggtatgg ctgtcttttc
     2341 ctgtggcaac gtttcccgga tacacgaatt tcaaaccgct ttccaaggct atattcctgg
     2401 catggataac agactctatg ggggtgtgcg ccacgtcctt aagcttgtaa gcggggaaga
     2461 agcggctgat atggagcgga actgtttcaa aaccgttgcc cgccagccac cggcacatgg
     2521 atttgaattt tgccggatca tcggtaagcc ccggaatgac caggtgtgtg atctcaatcc
     2581 acacaccggc tttctttaaa gtcagcaatg tatccagcac gggttgaagt cttccgccgg
     2641 ttaggcggcg gtaagtcttg tcatcaaagc atttgaggtc aatattcgcc gcatccaggt
     2701 aaggtatcag ggtggccagc ggctccggat tgatataccc gttggacacc gccacggtct
     2761 tcaggccttt ttcccttgcc acaacagcgc tatcatacat gtattcgtaa aaaacggtag
     2821 gctctgtgta ggtaaaagca atggaaggca cgtggcaatt cacggccatt tccgccacct
     2881 ggtccgggag catgtcataa ctatcatacg acaaaggaga aacctgcgat atggaagagt
     2941 tttgacaatt aagacagtgg aaattgcaac cggcagtggc aattgaaagt atttcggtcc
     3001 cgggtaaaaa atgatataat ggtttttttt ccacaggatc aacggccacc gaacagggat
     3061 tgccataagc aacggaatac aaggtccctt ccttgttcgt tcttgtgtgg cacactcccg
     3121 tttctccatc ccttatgagg cattcatgag gacaaagcaa gcacttcacg gttgttcccg
     3181 ccatggttct ccaacggttt tcccctacct tgtaaaacat cttgttgctt aaaaatcaca
     3241 agataaagaa aaaagccgtt gatatgaagc ttttgaggtg gaacctcttc gcgttacttg
     3301 cttcgggcag cccctgtatt gcaaagaact gaacactttt ttggtacgcc gaggcgctca
     3361 gttctttgca gtgcaggaac cttgccgttg agcagcattg ctggtcaata accgcatagt
     3421 tgtctttcag gtggtgcgca acgcacacat tgtcctttta tctgacaatc agagcattat
     3481 atcattcata cccccaggag tgtgaaacgc gtaagtggct gattttgagt cgaatgaatc
     3541 aaatttgcgg ggcacttata cctagctgct gataccacaa gaaaagggaa gcctcggcct
     3601 aaaagataaa gcttcacgtt cttgtggtat cagcggcact atgaaattat tatcaatgcg
     3661 ttgctgacat actgatagtt atggccgcat taaatttgaa gggaaatctt cagaaaaaaa
     3721 gggctgtccc agcacttgtg ctcccttctt caggtacacc ccatgggaaa atccctccct
     3781 tatgccccgt acaaacaggt ataccacccc tccaaagtca cgggaagcat cgaatccact
     3841 gattttggaa ctcaggtagc gtaatgcagc catggtgtaa atattgtact gaaggtcata
     3901 gcggctttcc cttaccacgt tgtcaaccgc tgtttggttg taatcttccg gtgtgcttcc
     3961 catgtagttg gatttccaat caaggatgta gtacttgtcc ctgaaccgga aaaaaagatc
     4021 catgaaaccc atcatgagtc cttctgcctg caggttgtaa aagaaagcca tttccccggc
     4081 tttttcccga tcattgacct gtgccagcac caggtcttca cctatcctga catgaagcac
     4141 atggtttatc atggtcaata agtgttccct gtcttccacc gcacagcaat ggggaaacat
     4201 cctcattgcc ctgtcaacgg catccggcca tccggtggaa tcgtcaaaaa caatatgctg
     4261 aaacaagtgg tgaataaata cccctgtttt ggcgccacgg ggaagccggt caaaaatgaa
     4321 atcatcataa gcgctacgaa catctgtact gcctgcctgc agcaaaccgg gagatacagg
     4381 ttcctgaacc gggaggacaa aaggcatttc gccgaccagg gaactgaaac tgcggacacc
     4441 ccaagccccc tccatgggcg gtacatcatt gggttcacgg ctgaccggct tttctacgac
     4501 acttttcttg cggccgcctg cccatgtatg cgggattttc tcatctgaat gcccaagagg
     4561 ttcaatcaag ccttttatcc ctcctccaag agcgaccatg aaaggttgca gcgaacagga
     4621 tgtgtccttt gtataaacgt agcatttata tacagccctg gtaagagtca catagatcag
     4681 gcgtctgtgt tcctgtacaa actgattctt ggccatttgc acgaattcat ctgtcttgtt
     4741 tacggccatt acatactctt gtcttggata tggaagctga aaatcaataa attcacgttc
     4801 tttccagatc gtgttcagat acggcgcaaa tacaattttg taggtcaaac ccttgcattt
     4861 gtgtatggtt gtgatctgca ctgcgttttc atcactttca atccgctgct ggtattcatc
     4921 ttccacctta ttgtctcctg tgatctgcct ggaaagccag gaaatcagct catccggaga
     4981 aagggaggaa cggatttctg ccttgtgcag aagttccatg atatgcaggt aattggtaat
     5041 ggcccgctgt ccctgtaggt tgccttcggc ggtgcaatga tcgcgtaccc ggtatatttt
     5101 caaaaaggca gataaagcat tgtatatgcc ccattcactc catatcgcat gcatctcacg
     5161 gaagcgctcc agctcttttt cctcatccat gctaatgacc gcctctgtgt caaagccgaa
     5221 atagggattc agcagggctc ttcccaggct cactctgccg gaattgcgga tggccagtaa
     5281 aacattatgc agttgccttg cctggggcga agacaatacc cgggtatcgt ccagggttac
     5341 agccggcaca tttctgtgag ccagttcatt tcttatgatc tttgcctgct tgttagtcct
     5401 gacaatgatt cctatatccg atggcctgat ctgttccctg cctccgtccc gcgacgggag
     5461 tgcataacta tcagaggaaa gaatttccac cactttactg caaaccatat ccgggatgtt
     5521 ttccacgata tcgtaattac aaatgcttat gggtacagac tgtgttccct tttctgtcat
     5581 ttcggcccgc tttgttccgg ctctaactgg aaaatacagg atttccggat ccagaaaagc
     5641 attttccagg gaaaaaaagc tgttgagtgc atcaatcagt ctggaagttg acctgaaatt
     5701 gctgttcatg gtatagatgc ttttcacctg tccgcgggct gtcttgtagg tgttcaggtc
     5761 ggctttcctc cagccgaaaa tggattgttt gggatcgcct atgtagaaaa caacatgatc
     5821 atgcatttcc tgcaccgcgg gagaggaaaa aacgtcatgg aaaatcttgt attgctccct
     5881 gtccgtatcc tgaaactcgt ctatgaatac agctgcatat ttatcgcgca acaaccggca
     5941 gacactttcc ttttccacag ccttgctaag gctgttaatc aagtcatcga aaccaacaac
     6001 gttttttctg ccttttacct ccctcatgcg ttcttcagcc actgcctgca atttcataag
     6061 cagatgctcg accagggaag tcctgaaacg caacatttcc tgttgttgtt tgtagtatgg
     6121 ttcatacaac tcagcaatat ccggaaaaca ggcaaccagg tatttcttga cccacctggc
     6181 ttcaaacagg tcaatgaaat cctgtgccgt tgctgattgc tccagcagct tcctggcatg
     6241 tgcattcgtt tggtcctgcg ttctcatacc gggaaactgc gattccgctt tctgacagaa
     6301 caggcggtaa gcttcctctg ttttcttcag tgaaagcctg atttcctcca acacttccgg
     6361 atcagaaagc tccttcccgg tgacctccat tcctgacatt tttccgggca tagcatgttt
     6421 gtcttccagg accactgtcg agaaatctcc tggcccttcg agcggagcgg tgatgagctt
     6481 cttcccggac aggactttgt ccacgaacag ggtcaatatg cttttcttga atgggaattt
     6541 tcctttttta tacaacagca atttcaggag caccgggtca aggaccgaga cttcctccct
     6601 ccagaaccgg ttgcatacac gttcgttaag cagcgtctga tctgaaagca cctccgcatc
     6661 aaaagcctgc tcggtttcga aggcaaattc cagcagtgtc tgctggcaaa aaccatgtat
     6721 tgtcatgatc tgcgtatcgt ccagatctcg gactgcggtg gccagacgtt ccctgactgt
     6781 ttctttatcc ggcctgtctg tttgcccggt tccatcaacc acttttttta tacttacatc
     6841 aacccggtca tccctatgac aggcataacc gtatgcctgc cttacaaata agcggatgcg
     6901 caattccagt tcagccactg cagcatttgt aaaggtaacc ataagtatct gcttcactgt
     6961 aagacccttt tcaatgatca tcctaagtgc aagtaccgca atggaatacg ttttacctgt
     7021 gcctgcactg gcttcaatca ggttgctgcc ctccagggga acctgtagcg cgtcgaattg
     7081 cttcatgttt cccatggtgt aaggattaat gctgcatatt cgttcattag ctgctccaag
     7141 gcttcatgcc ggtcgtcggc aaacacaccc tcttcccagg ccagggcaac gtaagggtcc
     7201 ggttcggtag tacgcgtacc ttccgcttct tttttgattt cccgcagggc ctcattcaat
     7261 ggctcccctg aatccccccc cgacaagaag aatgcattgg cagctttcag ggtgatggga
     7321 agccgttttt cacaaccctg cctgaataat gccgttaacc gggacagttg ttccatagcc
     7381 tggtgttgag caatggcggg aatgtggaat tttttgccat gcaggtcaag gaacacggcc
     7441 tgaccggaga aaccgcaggc acacaggacc aggtgtctga tccttgtttt gacctggtgt
     7501 ttgagcgcgt cttttgatac ggtgaagtcg tataaggagt tgtcaaaaag gtttgttaca
     7561 gctcccctga caactattcc ccccactgtg atctccacct ggcaactttt tcctgtcttg
     7621 cctgctgtaa gctcctgcca tgatgcctgc aagtccccga tctccagctg agcctcactg
     7681 actttcattt cttttatgcg ggccagtggc atattgcctt ctttcatggt cttgtctgtc
     7741 cacgcagcta ccaattcctg ttctccaaca gggtcagtct gcaccaaaaa ccttttcaat
     7801 tcccagtact gcaaattatc caggtcaaag atctcgcttt ccggcagggg ttgtttgggc
     7861 tcgtcatagc ggatgcccaa tattttctgg taataccact ccagcggatg tgtgaagaat
     7921 tgcgccaaat cctccagttg caccgttccc gggatcgtgt ccccggctat gtctggttgc
     7981 aaggctttgc ctcccccgga ttggtccggc aaatcggaaa acaggtaggt gaacaggcgc
     8041 tcatcttttc cgtcgtaccg ggagctgaag ccatgcaggg gatgaacctg tgataccccg
     8101 caccggacca gctcccggct ttcttccgga acgagtgatt ccaggtaatc cgtcagctcg
     8161 tcaaggacaa ttgatggggg caggacagta ttatcttgta tgtctctgcc aatataactg
     8221 atatacagat aatccctgga agcgaccagg ctttccagga aaataagttt gtcgttgtct
     8281 tttttgctcc tgtcacccag gcggggttcc ttttggagca ggtcaaatcc cagggaagag
     8341 tctttacgcg gaaaaacgtt gccgttcaag cccagtatgg caatgacacg tttggggatc
     8401 ccccggcccg ggatgacagg caggaaattt acgtttccgg taatatacat gcccgagcgg
     8461 gtgtctgtga gcagggtgtc ggcgaccagg tgccggaaga ccttaaaaga caggggcgtt
     8521 gtttcgtaac tgcgttccag gatttccatg cggcggtata tgtggtccaa ttccatatcg
     8581 tcctgccctg aataggctac tgaacgttcg agcatctctt ccaggagaaa ggttttccag
     8641 tctgccaggg agcgatgctc tgtttttgtt cgcgcataga tggtcagatc gtccagaaaa
     8701 gctttcagcc tgagcaggtc ataagcaaca gaggtctcgg cttcgcgcca gggaaataag
     8761 gtataactgc cttgagacga aggaatggat tgttctgtta agatggcgaa tcccatcatg
     8821 attttttcaa aaccatattg ccagcttaca aaacgggtgt catcctctac cctgttttcc
     8881 aggccgaacc ggatattgag cgcctctatg gcatcgcgca ggtactgcat gtccgtgatc
     8941 ccgtatttct ggcagatgcg tttggtttcc agcaggctta ccacctcttc ggcagtaaaa
     9001 ccatccagcg cgtcaaaatc catcaaagcc atcaatgccc cggcaatgga atcctgctgg
     9061 tcttgggatt caccggcaat aaagaaagga atgggatatg gaccattgcc aaaaactgct
     9121 ttcacataag gggcataggt gtccacatcg ggcaggagca ccagcacatc ttcgggctta
     9181 agccccgcac cggcatgcat gagggagagc agataattgt aaaacacctc cacttcacga
     9241 accggtgtgt aacaggcatt gatcaccagt gttccatccc ctgtgtcttt ttccgtcaat
     9301 gtacccggct gcgggacaag gtccctgcgc agggtgttcc tgaacttgcc caaaagcgtt
     9361 tcaccggagt gctgtcccca caggggcacc acttcacatt cccggggcag catggccgcc
     9421 atctcggcat actttcttcc caacgaagcg ctcagcgggt tggaaaaatt ccctttttca
     9481 aagaaatgtt ccgaggggag aaccaggtaa tacgccacgt caatgatgcg ggccagtttt
     9541 tctaaaaccc ggttgtggaa ggttgtgaaa atggataacc cgaagagcga gagacgtggc
     9601 agcatctggc ggattttggg ccaattctgc ggttcatcca gcagttcaag caaacgggcc
     9661 tgcatgcgat gttttggaca gaaccaggga tatttttccc tgacggaatt ccacaaccac
     9721 cgctgccatg cttcatggtc cgagtcctga gggtcgcccg ggctgctttc ctgatccccg
     9781 tccggtggat aagaacgtct gtccggtgca gattcccagg ccgacatcat gtcggggcgg
     9841 tatacctggt actggtcaaa taggtcggat gttttcccgg caagctgcat gcgcctgagc
     9901 tcattgtttt gtttatcctg tgagtaatat gctgcaatgt ccgggaaacg ggttatgaaa
     9961 gattcgctgc ccaggagctc atagatagca gggcgtatgt tggtcgtata acgcttcttc
    10021 ccgtaaagtc ctgcatatgc ctcttccagc agctggtcct gtttcatgaa gcggaaattg
    10081 gcaaatacgc cgttcctccg ggtcagctcg gtggacagcc agctttccat gccggcagtc
    10141 tgggttatga gaacttcagg caggaatatg gtgtctgcat ccgccgaagg ataatccatg
    10201 cggtcgatct gctgtgccag tttcccgcaa aggatttcca attggtcaga tacatagatc
    10261 acagctgcca taaaactcat gcttttaaac ggttagccag gccggatacg cgggatatcc
    10321 ttttgcgcac ggtttcttcc agcacatcct gcggagcctg gaccaggacc cgcttttgtc
    10381 ccctggttac ggctgtataa agtaattccc gggtaaggat ccttacatcc ttgttatcgg
    10441 gcaaaagcac cagcacattt tcaaattccg acccctggct tttgtttatt gtcatggcaa
    10501 agacagtctc ccaatgggtc aggtacccgg gcaggatgcg catcaaattg ccatcgggcc
    10561 cgggaaacca tgcatagagc cggttgccgg gatccagcgg atcgcgcctt atgatgccca
    10621 catccccgtt aaacactttt agcgcataca tattggaagt taccatcacc ggctggttgt
    10681 ggtaaaaacc ggcagacggt tctatgggaa gtttcaatga gcacaaataa ccggcaatgc
    10741 gccggttggt ctgatacaca ccatgctctc cttccctgat ggcgcatagt acgcgaattt
    10801 tcccgatttt ttccagcgct gtcgcaatgt ccggttcagt tatataatcc ctgtacatta
    10861 cggcaaattc atggaaaagc ttatcgtcat acgtggtgtc aatggatagg gcatcatccg
    10921 gatgatccat ggtacgcaca atgcagtcgg aatcgccggc cagcacggaa cggctgaaaa
    10981 gccctatgcc tttgtcggca tcgaaccgat ggctctttgt caaacggatc acgtgtccat
    11041 ccagcacaga ccctgtccct tctgtcttgc agagatctcc aaaaacgctc ccggcttcaa
    11101 tggaggccag ctggtcctta tctcccagca gtatgagtcg tttttcggga ctcgtggcat
    11161 tcagcagttt ggccataagg gccccgtcta tcatagaagc ctcgtcaact atggtgatat
    11221 cgtggttcat gggattttct tcatcgtgcc tgaagaaaat gctgccattt acagatccca
    11281 ggagccggtg gatggtagat gccatgggca gcccgggaat ttgctgcctc agtacggtca
    11341 tggactcatt gatcctggct gcggcttttc ctgtgggggc agccagggca accttcaggt
    11401 caggttgccg ggcatacagc acacggagca cctccctggc cagggtggtc tttcctgctc
    11461 cgggccctcc ggtgattatg cagaagttcc ccagcagtgc cggacggaat gcttcggcat
    11521 tttccttgaa aagttccttg ataagcggct gcagtggctc cagttccctg atccgttggt
    11581 tccggagttt gcttccttcc gctatgagtt gacggatttt ttgctctatg ttcgtttcat
    11641 agagaaaata acgttgaaaa tacaggttgc ccaggtagac aataaagggc ttgagagcgg
    11701 aggggtcatg cgacacatag ggaccgggaa gcagtatacc tgcaggaagt gcttcttccg
    11761 gatggtcatc gtccaaggtc ttcaaatcaa ggcacatatg accctcgtcc agccttttca
    11821 gcaaagcacc ggccaaaatt cccaatccag gctgaaactg gttgaaataa tcagaaaaga
    11881 tgcggtagac atccatcggc tcttattcgc ctatgatctt gaccaggatg cgtttttttc
    11941 gttttccgtc aaattcacca taaaagatct gttcccatgt cccgaaatca agacgtccgt
    12001 tggttacggc aatcaccacc tcccgcccca tgatctgacg tttaaggtgt gcatcggcat
    12061 tgttttccag gccattatgt cgatattgtg aatgaggtaa ttcgggagca agacgttcca
    12121 gccacttttc aaagtcgtgg tggagccccg attcatcatc gtttataaaa acgcttgaag
    12181 tgatgtgcat ggaattgcac agcagcagcc cttcctgtat cccgctttct tcaaggcatt
    12241 cctgaacccg ggacgtgatg ttcacaaaag cccggcgtgt gggggtttct acgtaaaatt
    12301 cctgtctgta tgttttcatc tttttaagtt atctgtgtgc taaagataca aaaaaacatg
    12361 ctttataatg attttattgc acccctgacg ggatattact cgttctgttt gtcggctgaa
    12421 cataattttt tcgtatattt cgcctgatga tgaatcctgt tcccaccaac ggttattgct
    12481 tagtaaccgg ggctgcacac ggtcttggga gggctttcgc attggaactg gccggcagac
    12541 atctgaacac cctgctggta gaccttcccg gcaatgggct taaggaactg tgctcacaca
    12601 ttcatgagaa atacaacgta ttgtctcatt gtatcgagtg tgacctgacc aaaaaagaac
    12661 aggtccttga ccttgggaca agcatcaaca ggcaattccc cgtattcatg ctcatcaaca
    12721 acgtgggcac gggaggaagt gtcagattcg aagaagccga taccgattat ctggacagga
    12781 tcattcagct aaatgtcatg gctaccacgc tgcttaccag gcaactgatc cccaacctgc
    12841 tggcacaaaa gaaagcctat attctgaacg tttcaagcat ggccgctttt tcacccatgg
    12901 gttacaaaac catatatccg gcttcgaagg tttttgttca ttatttttcc aggagtcttt
    12961 accaggaata caagcacaca aacctgtttg taagtgtggt gaatccgggc cccatgaaaa
    13021 caaacccgga tgttaccaga aggatagaaa agcagggctt cttcagtcac tggggcgtga
    13081 tgtcacctga aaaagtggcc gctatttcca tccgacagct ttttaaacag gacactctga
    13141 tcatgctgag tttagtcaat gggctggaat ggctggctct caaacttgtt cccatataca
    13201 tacgcctgcc cttattgagc cgcgccatga aaaatgagat taatattgtc taaatgagaa
    13261 ctgttctggt cacgggtgct aacggacttt tgggaacaaa tgtggttctt gaattgctca
    13321 gccgcgggta tgctgtgaaa ggacttgtac ggtcccgccg acggttcatg gattattccc
    13381 atcccttgct ggaactggtc attggcgaca tcagggataa gacttcattg caggctgctg
    13441 cccggggatg tgactttgta gtacatacag cggcagatac ttcccaaaaa catctgaagg
    13501 tgacggatta cgatgcggtg aatcttgagg gcacgaaaaa tatcctggac ctgtgccggc
    13561 aagaagggat cagacgcctg gtttacatcg ggacggccaa cacatgcggt tttggatcaa
    13621 gggaaaaccc gggagatgag cagtctcccc cggcgccccc gttcaccggc tgtctttatg
    13681 cccttagcaa gataaaagcc ggggaactca tagacactgc tgtatcagaa ggtatggata
    13741 tcgtgaccat aagccccaca ttcatgatcg gcccgtatga ttacaaaccg tcctcgggac
    13801 gtataatcaa tatggcgctg aacaaaaaag tcgtatttta tccccccgga ggaaagaatt
    13861 tcgtccacgc tgccgatgtg gccaggggtg ttgtcaatgc tcttgaaaaa ggtgtagcgg
    13921 gagataatta cctgatggcc aacgaaaacc tttcataccg ggattttttt cagatcattt
    13981 cccggcttac cggcagtcgt ccgctgatga tccggctccc acgcccgttg ctgttgatct
    14041 tgggtctcgc cgggaatttc ctgaggcgcc tgggggcaga gataccgctt tgcagcaaca
    14101 atatgaaaat cttgtgtatc aacaattttt atacaaacca gaaagccctg tcccggttta
    14161 acatgtcctt tctcaccaca gaagaggcgg tgcgggacgc gttacggaca ggacctttta
    14221 gagccatatt aaagaattaa tcactgagtc ccctttaaaa agtggcaatt tgaagtttca
    14281 aaaataagta tgatgattt
//
LOCUS       DAOSOF010000040        14038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4364_length_14038_cov_4.240363, whole genome shotgun sequence.
ACCESSION   DAOSOF010000040 DAOSOF010000000
VERSION     DAOSOF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_14038_cov_4.240363"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="lysA"
                     /locus_tag="PLM68_03640"
     CDS             <1..901
                     /gene="lysA"
                     /locus_tag="PLM68_03640"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT54491.1"
                     /translation="IVYAGVGKSDREIRTALQGGIFCFNCESIPEIRIINDLASQMGK
                     TARISLRLNPNIDAHTNKHITTGRSRDKFGISEWMLDEVLGIIAASRNIKLIGLHFHI
                     GSQVEDMQVFEKLCKKVNSYQKWFEQHDIHLEHINLGGGIAVDYENPIENPFSPFETY
                     FETINSNLEVRKGQKVHFEPGRALIAQSGYLISRVLYVKIGRGKKFAILDAGMNDLIR
                     PALYGANHAIFNITSASQKKEVYDVVGPVCESADRFGLAVKLPETGRGDLIAICSTGA
                     YGQVMGMRYNQRELAQAYYSPDL"
     gene            910..1527
                     /locus_tag="PLM68_03645"
     CDS             910..1527
                     /locus_tag="PLM68_03645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT54492.1"
                     /translation="MYIRIYPNNPQDRLVQLTVATLQKGGIVIYPTDSVYGMGCSIEH
                     LGSVPRMERIKGYYTPSTRLSFLCYNLSQVSNYCRPISNAVFRLIKKNLPGPFTFLLQ
                     GNSRVPRLFKGKKQTVGIRVPDNPILQVILRELAVPIISTSLPWDETEPGYSSDPDLI
                     NERFGELVDIVIDGGPGGQDPSAIIDCTGPEPVIIRGGPKPLLDR"
     gene            1530..2867
                     /gene="ffh"
                     /locus_tag="PLM68_03650"
     CDS             1530..2867
                     /gene="ffh"
                     /locus_tag="PLM68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT54493.1"
                     /translation="MFENLTERLERSFKLLKGEGKITEINVAETLKDVRRALLDADVS
                     YKVAKGFCDQVKEMAMGQEVLKSVRPGQMMTKIVHDQLVALMGGDASGINVKGNPGIV
                     LVAGLQGSGKTTFSAKLASHLKKKKGMRPLLVAADVYRPAAMEQLKILGQQAGADVYM
                     EEGSMDPVKISLNARDKAKKEGYGVVIVDTAGRLAIDNTMMQEITAIKKALNPSETLF
                     VVDSMTGQDAVETARAFHEQLNYDGVVLTKLDGDTRGGAALSIRAVVSKPIKFVSTGE
                     KMDALDVFYPARIADRILGMGDIVSLVEKAQEQFDEEESKKLRKKLAQDSFNFNDFLS
                     QIKQVKKMGNIKDLASMIPGVSKAIKDVDIDNDAFKGIEAIISSMTPEERDNPSLLNG
                     SRRKRIAAGSGTSITQVNNLVKQFDDTRKMMKKLGAGGGASMANALRKGGIRK"
     gene            2864..3742
                     /gene="folD"
                     /locus_tag="PLM68_03655"
     CDS             2864..3742
                     /gene="folD"
                     /locus_tag="PLM68_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOT54494.1"
                     /translation="MILLDGKSAAEAMKKDIAREVIRWCKESGRRPHLAAILVGEDPA
                     SLTYVRNKENDCRQVGMDSSVYRLSSTISQEGLFECIRTINEDKDIDGLIVQLPLPDH
                     LDEQEIIEAIDPAKDVDGFHPVNVGKMVLGLPSFVSATPAGIIDLLAFYRIPTTGKEC
                     VIVGRSNIVGRPLANLLSRKGSGGDCTVTLCHSRTTDLAKVCRRADILVAALGQPHFL
                     KANMIKEGAVVIDVGITRIADKSTKSGFRLAGDVDFEEVAPRCSYITPVPGGVGPMTR
                     VSLLKNTLQAALKRNQ"
     gene            3754..4701
                     /locus_tag="PLM68_03660"
     CDS             3754..4701
                     /locus_tag="PLM68_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT54495.1"
                     /translation="MERRNFLKTAILGSIAGVAGLKSTRLLARNTSSREEAKQSGSFP
                     DLVAVMGGEPEQLYARAIQEMGGMKRYIKPGMKVVVKPNIGWDKKPEFAACTNPGLVA
                     AIVRDCLAAGASEVKVFDHTCDQWRGCYENSGIQEAVLQAGGKMVNAHEERDYRETDL
                     PAGIRMKKAKIHEALLDCDAWINVPILKNHGGARMTICMKNYMGIVWDRRYIHSNDLQ
                     QCIADAATFAKRPVLNIVDAYRIMTQNGPKGRSLEDVQTPKALFMSSDIVAVDTAATQ
                     FFNQFSSMSIQDASHIVLAEKHKLGTTNLDKLNIKRIIL"
     gene            4818..6200
                     /locus_tag="PLM68_03665"
     CDS             4818..6200
                     /locus_tag="PLM68_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT54496.1"
                     /translation="MIKLQIVPAILGIASGGLLTVIIVLLLTLIFGRVYCSLLCPLGI
                     WQDIVNRSASFFRSRKKRRYRFTKAHNYLRYTILTAAAVCLVFSFTLPILWLDPYALS
                     GRMAVNLGKPLVSLISGSQYAPWSLAAFLVSAVIFLTITLLAFFRGRLYCNTICPAGT
                     MMGLISKISMFRIQIDTAACTHCKLCGTVCKCGCIDCNSSFIDNSRCVVCMNCITACR
                     RGDIRYRFAWKRKNSEHKSENNKQHDKSRRAFLIAALGGGAGAGAVLLSRYAGKKRVR
                     ITSSMPPGAGNLERLKDKCIACHACIAACPSHIIKPAMGDFGWNGFLLPAVSYENGFC
                     GFDCQRCQEVCPSGALQLMPLEEKKRFRIGVVKLTLERCIVEEFGTDCGACDEHCPVK
                     AVQMTPREGTNKVFPATNPAICIGCGGCEYICPATPKAIEVIPLPEQRLADPPVLDAK
                     VKVVIDGFGF"
     gene            complement(6197..6886)
                     /gene="trmD"
                     /locus_tag="PLM68_03670"
     CDS             complement(6197..6886)
                     /gene="trmD"
                     /locus_tag="PLM68_03670"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT54497.1"
                     /translation="MRIDIITVLPELLKSPFSHSIVKRAVERSLVQIVVHDLRLYGKG
                     KYRQVDDYSFGGDAGMVLMIEPVYRLMEDLKKERTYDEIIYTTPDGLPFNQKEANRIS
                     CLHNIMILCGHYKGVDQRIRDHLITRELSVGDYVLSGGELAAAVIADAVIRLLPGALT
                     DQTSALTDSFQDGLLAPPIYTRPANFMGWKVPDVLLSGDPVRIGEWQEEMSLGRTRRL
                     RPDLLDEADLD"
     gene            6972..8741
                     /locus_tag="PLM68_03675"
     CDS             6972..8741
                     /locus_tag="PLM68_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOT54498.1"
                     /translation="MDPEKNTSAPEEQFDNKTPQNETVVETTQITDAIDYQTLSIPEL
                     LAEFERLIEAEDKTEMHKNADIIKSCFYKLLRKDQPVTAPDQEAPELAQEDPDETAFK
                     RLYARYKVLRADLLRNIEEQKERNLEEKLSIIEGIRALLEKQEDINHTFPEFRALQLH
                     WKEVGPVPLSRTKDIWETYQHCVEKFYDYVKINNELRDLDFKRNLEIKEKLCEKAEAL
                     VEEPNVVSAFHKLQKLHEEWRETGPIARELRESMWERFKISTTAINKRHQDYFEEQKQ
                     QQRKNLEAKLAFCEKAEAIAKELTDDDVNWNQKAKELEALQKEWRTIGFATKKENQKV
                     YDRFRNACDQFYGEKRGFYSKFKKEMQENLALKIALCEQAEAINMSDDWRRTTDQLIH
                     LQKQWKDIGPVPRKQADAIWKRFRSACDVFFTRKAEHFSKIDTVYLENLAAKEALLKE
                     LDNFDQSDVKDLQAALRDFQNRWNQIGFVPGKEKERINNEYRNLINLHFKNLNPGFSE
                     KRTQYRTGRGRSDEFRSGRDKLIQRFRQLESEISVWENNMGFFAKSMNADKIIADTCR
                     KIETAKQELAELESKIKQLEDNE"
     gene            8734..10602
                     /gene="yidC"
                     /locus_tag="PLM68_03680"
     CDS             8734..10602
                     /gene="yidC"
                     /locus_tag="PLM68_03680"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT54499.1"
                     /translation="MNKNTVWAILLIGAILVGFSLYNSSVAKKQQREQFVRDSIAREV
                     TIEAIRLEEQNKPENLPQAEDQMIQPSVTAPGQRYTNPFLNQSLEGEESFYILENDKL
                     RISFTNKGGQPYSLQIKNFKTYAGDSLIFFSGPSNKLALDLYAGEQINTTDFFFVPHF
                     SADNNSLTFSLHFDEQSYIEYIYVLEKDSHLLNMDIKLTGMDRYIPRNVTQLDLTLNL
                     DIRHLEKGFDNEKNYSTISYKYPNTTDVEKLGQRKAEASESIKTRVEWVAFQQQFFSA
                     ILISDQNFNSGDLSFRFYDAANPDTLLINCRAYMQIPFQASAEQSVPLRFYFGPNGFY
                     SLKAFDRSFEKIIPLGGFLISWISRFLIIPTFNFLNRFISNYGLIILIMTIFIRLIIS
                     PFTYRSQMSSAKMRVLKPEVDKINAKYPKKEDAMKKQQETMALYKKTGVSMMGGCWPM
                     LFQMPILFAMFRFFPGSIELRQQGFLWADDLSGYDSILNLPFKIPLYGDHISLFALLM
                     ALSMFFYSKMNMKEQSSGQQMPGMQGMMLYFMPLMMLVVCNNLSAALSYYYLLSNLTT
                     MLQTWIMQKFVVDEAKIYQQLKDKADKAPSTPKKSKFQQRLEEMQRQQQQQMKKRR"
     gene            10599..11258
                     /locus_tag="PLM68_03685"
     CDS             10599..11258
                     /locus_tag="PLM68_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT54500.1"
                     /translation="MIARNLHRLYQHIPKSVTLVAVSKFQPVPFILEAYGSGQRCFGE
                     SRSGEMLQKIPLLPQDIQWHFVGHLQTNKVKEVVGKASLIQSVDSVRLLNAIQQQAHQ
                     LGIVQDVLLQVHIAVEETKHGFLPSELQQVCSLSLPAVRIRGLMGMATYTEDNDRIRS
                     EFKTLRQLFDTLRSGPFAGKDDFTHCSMGMSGDYQIALEEGSTMVRIGSAIFPSRPQR
                     P"
     gene            complement(11227..12018)
                     /gene="dapF"
                     /locus_tag="PLM68_03690"
     CDS             complement(11227..12018)
                     /gene="dapF"
                     /locus_tag="PLM68_03690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT54501.1"
                     /translation="MKLPFCKYHGAGNDFIIIDNLSGQYELTSFQISALCHRRFGIGA
                     DGLMLLERADAPWNYSMAFFNLDGTSGTMCGNGGRCLVAFANHLGINKHSFTAVDGAH
                     QAYVIENKDNYSRVKLQLNNVDGIKPYQKDYYIVDTGSMHLVCFVPDVKAVDIATEGP
                     YWRHHKDFGKKGINVNFVEILDTGCLFVRTFERGVEDETWACGTGSTAAAIAASVHTG
                     TGLNHWHIQTKGGNLEVSFQKNDNAFTEVFLTGGAVRVFEGVMEI"
     gene            12082..13530
                     /locus_tag="PLM68_03695"
     CDS             12082..13530
                     /locus_tag="PLM68_03695"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT54502.1"
                     /translation="MKKRIGIIVIATAIVSVGATILVHSLTENESKVEKVYLTSAQEP
                     VIHNVSLPEVQYPDFTYAAETGVKAVVHVKVVKHGQQAQGPQSLFDFFFGYGSPSPQP
                     RPIVGAGSGVILTSDGYIVTATHVIEGADDVLVTLDDKRQFEGKVIGKDPATDIALLK
                     IEATNLPYLQFGNSDDLRLGQWVIAIGNPYDLRSTITAGIVSAKGRSLPSYNGEFKIE
                     AFIQTDAAVNPGNSGGALVNAKGELVGINTAIATRTGSYSGYSFAVPSSITKKVVDDL
                     RKYGIVQRALLGITMQDITGDLAAEKNIKDIRGVFIHELVTDGAAEKAGVKPGDILLQ
                     VEGVPVNSGTAVQEQINRFSPGDKIDLLLLRGNKEVKVTATLQNQSGDTELIKEGTGD
                     LTFFLGAQLRPATKEIRDQLKIRAGIEVVSIEEGKLRDAGIRKGFVITHINQKQVGTV
                     QQLALAIQTAQRGVLIEGRYPDGSVYYYALSN"
     gene            13608..>14038
                     /locus_tag="PLM68_03700"
     CDS             13608..>14038
                     /locus_tag="PLM68_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54503.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLISVEEEVELAQRIK
                     KGDQEALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINRALS"
BASE COUNT         4062 a         3111 c         3394 g         3471 t
ORIGIN      
        1 cattgtctat gcaggtgtcg ggaaatccga cagagagata cgcacggctt tgcagggcgg
       61 tatattctgt tttaattgtg aatccatccc cgagatccgg atcataaacg acctggcgtc
      121 gcaaatgggc aagactgccc ggatttctct ccgcctgaac ccgaacattg atgcccatac
      181 gaacaagcac attacaacag gacggtcaag ggataaattt ggtatcagcg aatggatgct
      241 ggacgaggtg cttgggataa ttgccgccag caggaacata aagctcatag ggcttcattt
      301 tcatatcgga tcccaggtag aggacatgca ggtatttgag aaactctgca aaaaggtcaa
      361 ttcctatcag aaatggtttg aacaacatga tatccatctg gaacacatca acctgggtgg
      421 tggcatagct gtggattatg agaatcctat cgagaacccc ttcagtccct ttgaaacata
      481 ttttgaaacg attaacagca acctggaagt cagaaaaggc caaaaagtcc actttgaacc
      541 aggacgggcg cttattgccc aatccggata tcttatttcc agggttttgt atgtaaaaat
      601 cggcaggggc aaaaaatttg ctatacttga tgccggaatg aacgatttga tcagaccggc
      661 gctatatggt gccaaccatg ctattttcaa cattacctcg gcatctcaga aaaaggaagt
      721 ctacgatgtg gtgggtcccg tttgcgagtc ggccgaccgg tttggtcttg ctgtcaaatt
      781 gccggaaacc ggccggggtg acctgatagc gatctgttcc acaggcgctt acggtcaggt
      841 tatgggtatg cgttacaacc agcgggagct ggcacaggcc tattactcgc ccgatttgta
      901 gtacccgtta tgtatataag gatatatccc aataacccgc aggaccgtct ggtgcaattg
      961 acggttgcga ccctgcagaa gggaggtatc gttatatatc ccacagacag cgtgtacggc
     1021 atggggtgtt ccatagagca tttaggcagt gttccccgca tggaaaggat caaaggctat
     1081 tatacccctt caacacgttt gtcattcctc tgctataact tgtcccaggt atccaattat
     1141 tgcaggccga tcagtaacgc cgttttccgt ttaatcaaga aaaaccttcc gggaccattc
     1201 acctttttgc tacaggggaa cagccgggtc cccaggttgt tcaaagggaa aaaacagacc
     1261 gttggcatca gggttccgga taatcccata ttgcaggtca tcctaaggga acttgcagta
     1321 cccatcatat ccacgtccct tccatgggat gaaactgaac cagggtattc cagcgatccg
     1381 gatttgatca atgaaaggtt cggcgagctt gtcgatatcg taatagatgg cggaccggga
     1441 gggcaggacc cctcggccat tattgattgt acaggtccgg agcccgtgat tatcagggga
     1501 ggaccaaaac cattacttga cagatagata tgtttgaaaa cctgacagaa agactggaac
     1561 gatcgttcaa attgctaaag ggagaaggaa agatcaccga gatcaacgtg gccgaaaccc
     1621 tgaaggatgt gcggagggct ttgctggatg ctgacgtaag ttataaagtg gccaagggct
     1681 tttgtgatca ggtaaaggaa atggccatgg gccaggaggt tctcaaatcg gtgcgccccg
     1741 gccagatgat gaccaagatc gtccatgatc agctggtggc cctgatggga ggagatgcct
     1801 cgggaatcaa tgtcaagggc aatcctggta tcgtgctggt agcgggattg caaggctcgg
     1861 gcaaaactac tttctcggca aagctggcct cgcatctgaa gaagaaaaaa gggatgcggc
     1921 ccctgctggt tgccgctgac gtataccgtc cggctgccat ggaacaattg aaaattctgg
     1981 gacaacaggc gggtgccgat gtttatatgg aagaggggag catggatcct gtgaagatct
     2041 ccctcaatgc aagagataag gcaaaaaaag aaggatacgg tgtggtaata gtcgacacag
     2101 caggacgtct tgccattgac aacaccatga tgcaggagat tacagccatt aaaaaagcgc
     2161 ttaatccctc agaaactttg tttgtggtag attccatgac aggtcaggat gccgtggaga
     2221 cggcccgggc attccatgag cagcttaact atgacggagt ggtccttaca aaactggacg
     2281 gagacacacg tggcggtgcc gcactttcca tccgtgcagt agtgagcaag cccatcaaat
     2341 ttgtgagcac aggggagaaa atggatgccc tggatgtgtt ttacccggcc cgcatagccg
     2401 accgcatact gggcatgggg gatattgtga gcctggtgga gaaagcgcag gaacaattcg
     2461 atgaggaaga aagtaaaaag ctgagaaaga aactggcgca ggacagcttt aatttcaacg
     2521 actttctctc acagataaaa caagtcaaaa aaatgggcaa cattaaggac ctggcttcta
     2581 tgataccggg cgtatccaag gctattaaag atgttgacat tgacaatgat gcattcaaag
     2641 gaattgaagc tatcatatct tccatgactc ccgaagaaag ggataatccg tcgctgctca
     2701 atggcagcag gcgaaaacgg attgctgcag gaagcgggac ttccataaca caggtaaaca
     2761 acttggttaa acaatttgat gataccagga aaatgatgaa gaagctcggg gcgggaggag
     2821 gtgcgtcaat ggcaaatgct ttaagaaaag gaggaatcag gaaatgatat tgctagacgg
     2881 gaaatcggct gcagaagcca tgaaaaaaga catcgcccgg gaagtaatcc gctggtgcaa
     2941 ggaatctgga agaagaccac atctggctgc cattctggtt ggcgaagatc cggccagcct
     3001 tacttatgta agaaataagg agaacgattg ccgccaggta ggcatggatt cttctgtcta
     3061 cagactttca tccacaatca gccaggaagg actgttcgaa tgcataagga ctattaatga
     3121 agacaaggat attgacggac tgatagtgca gttgcccttg cctgaccacc tggacgaaca
     3181 agagataatt gaagccattg atcctgccaa ggacgttgac ggattccatc cggtcaacgt
     3241 gggaaaaatg gtgcttggtt taccctcatt tgtttctgcc acaccggcag gaatcataga
     3301 ccttcttgca ttctaccgga tacctaccac gggaaaagaa tgtgtgattg ttggaaggag
     3361 caatatcgtg ggaagaccgt tggccaacct cctgtcaaga aaaggttccg gaggagattg
     3421 caccgtaacc ctgtgtcaca gccgcacaac agatctggcc aaggtctgcc gtagggctga
     3481 tattctggta gctgccctgg gtcagcctca ttttctcaag gccaatatga ttaaagaggg
     3541 tgctgttgta attgatgtgg gtataacacg catagcggac aagagtacaa aatcgggttt
     3601 caggctggct ggagatgtgg attttgaaga agtggctccc cgttgcagtt atatcacccc
     3661 ggttcccgga ggagtaggtc ccatgacacg cgtttcatta ttaaaaaaca ctttacaagc
     3721 tgcccttaaa cgtaatcagt aataattatt attatggaaa gaaggaattt tttaaaaaca
     3781 gccatacttg gttcaatagc cggagtggca ggtttgaaaa gcaccaggct tcttgcacgg
     3841 aacaccagtt ccagggaaga ggcgaaacaa tcagggagtt tcccggatct ggtagctgtg
     3901 atgggaggag aaccagagca gctatatgca agagccatac aggaaatggg tggcatgaag
     3961 cggtacataa aaccgggaat gaaagtagtt gttaaaccga atatcggttg ggataaaaaa
     4021 cccgagttcg cagcctgtac aaatcccggc ctggttgccg ccattgtcag ggattgtctt
     4081 gctgcagggg cttcagaagt caaggttttt gaccatacct gtgatcaatg gaggggttgt
     4141 tatgaaaaca gtggcataca ggaagccgtg cttcaggcgg gaggcaagat ggtgaacgcc
     4201 catgaggaaa gggattaccg tgagacggat ttaccggcag gcatacgtat gaaaaaagcc
     4261 aagattcatg aagcactact ggattgtgat gcctggatca atgtccccat tttgaaaaat
     4321 cacggaggtg ccaggatgac catatgtatg aaaaactata tgggaattgt atgggaccgc
     4381 aggtacattc actccaacga cctgcagcaa tgcattgccg atgcggctac atttgccaaa
     4441 agaccggtcc tgaatatcgt ggatgcatac cgcatcatga cacagaacgg acccaaggga
     4501 agaagtcttg aggatgttca aacacccaaa gctctcttta tgtcctctga tattgtggct
     4561 gtggatacag cagctactca atttttcaac cagttcagtt ccatgagcat tcaggatgca
     4621 tcccacatag tgctggcaga aaaacacaaa ctggggacta caaatctgga taaactcaat
     4681 atcaaacgta taattctctg atgcagcttt atggattttt aagatggttt cgcagggtta
     4741 ctgcattcgc tgtatttctg ggcctcttgt atttttttct tgcctcttct gcacgtcttg
     4801 caatcatatg gcaggaattg ataaaattac agattgtccc agctatacta gggattgctt
     4861 caggtggcct gctgaccgtg atcattgtac tgcttttaac cctgatcttt ggccgggtct
     4921 attgctcgtt gctgtgcccg ttgggcatat ggcaggatat tgttaacagg tctgcttctt
     4981 ttttcaggag caggaaaaaa cgcaggtacc ggtttaccaa agctcacaat tatttgcggt
     5041 ataccatact taccgcagca gctgtctgct tggttttttc ttttacactg ccgatcctgt
     5101 ggctagatcc ctacgcactg tccggcagga tggcggttaa cctgggaaaa cccctggtca
     5161 gcctgatatc cggttcgcaa tacgcaccct ggagcctggc tgccttcctt gtctctgctg
     5221 taattttcct gacaataacg ttacttgcct tcttcagggg aaggctctat tgcaacacca
     5281 tatgccccgc gggaaccatg atgggactga tctcaaaaat ttccatgttc aggatacaga
     5341 tcgataccgc agcctgtaca cattgcaagc tttgcggcac tgtatgcaag tgcgggtgta
     5401 tcgattgcaa cagctcattt attgataatt cccgttgtgt tgtatgcatg aattgtatta
     5461 ccgcatgccg gcgcggggat atccgttaca ggtttgcctg gaaaaggaaa aacagcgaac
     5521 ataaatctga gaataataag caacatgata aaagcaggcg ggcttttctg attgccgccc
     5581 tgggaggggg ggcaggtgca ggggctgttc tgctgagccg ttatgcagga aaaaaacggg
     5641 tgaggataac cagctcaatg cctcccggtg cagggaacct ggaaaggctt aaagacaaat
     5701 gcattgcctg tcacgcctgt attgcagcgt gtccttcgca tataatcaag ccggcaatgg
     5761 gtgatttcgg gtggaatggc tttttgttgc ctgccgtgtc gtatgaaaac ggcttttgcg
     5821 gttttgattg ccagcgatgt caggaagtct gcccttccgg ggccttgcag cttatgccgc
     5881 tggaagagaa aaagcgtttt agaattggcg ttgtcaaact tacactggag cgctgcattg
     5941 ttgaagaatt cggaacggat tgcggtgcct gtgatgaaca ctgtccggtc aaggcggtgc
     6001 aaatgacacc ccgggaagga acaaataagg tttttccggc aacaaatcct gcaatctgca
     6061 ttggatgcgg aggatgcgaa tatatttgcc ctgcaacacc caaggcaata gaagtcatac
     6121 ccctgcctga acaacgtctt gcagatccac ctgtcctgga tgccaaagta aaagttgtaa
     6181 ttgacggatt cggattctaa tccaaatccg cctcgtctaa aagatccggt ctgagcctgc
     6241 gggtacgccc caggctcatt tcttcctgcc attcgccaat ccttacagga tccccgctca
     6301 gaagcacgtc aggaaccttc cagcccatga aattggctgg ccgtgtgtaa atgggaggcg
     6361 ccagcaaacc atcctggaac gaatcggtaa gggccgaggt ctgatccgtg agcgctccag
     6421 gcaacagcct gatcacggca tcggcaatga ctgccgctgc cagttctccg ccgctcaaga
     6481 cataatcccc gacagaaagt tcacgagtta tcaggtggtc acggatccgc tggtccactc
     6541 ctttgtaatg tccgcaaagt atcatgatat tatgaaggca ggaaatccgg ttggcttctt
     6601 tctggttaaa tggcaaacca tccggagtgg tatatatgat ttcatcgtag gttctttcct
     6661 ttttcagatc ctccataagc ctgtataccg gttcaatcat caggaccata ccggcgtctc
     6721 ctccaaagga ataatcatca acctgcctgt attttccttt gccgtataac cttagatcgt
     6781 gcacaacgat ctgaacaagg gagcgttcca cggctctttt aactatagaa tgggaaaagg
     6841 ggcttttaag caattcggga aggacagtaa tgatatcaat tctcatgata aagcaaaagt
     6901 aatcataatt caatatattg tagtttttca ttacatttgc tggctataaa tacaaattct
     6961 ttcctattac aatggatccc gaaaagaaca cctctgctcc tgaagagcaa ttcgacaaca
     7021 aaacaccaca aaatgaaaca gttgttgaaa ctacgcaaat aacagatgca atagattatc
     7081 agaccctttc aattccagaa cttcttgctg aatttgaacg tctcatagaa gcagaagaca
     7141 agacggaaat gcacaaaaat gcagacatca tcaagtcttg tttctataag ctgctcagaa
     7201 aagaccaacc ggtaacagct ccggaccagg aagctcccga actggcacag gaagatcctg
     7261 atgagactgc attcaaacga ttatatgctc gttataaagt cctgcgtgca gacctgttgc
     7321 gaaatattga agaacaaaag gaaagaaacc tggaggaaaa actgtccatt atagaaggca
     7381 taagggcctt gttggaaaaa caggaggaca tcaatcatac attcccggaa ttccgtgctt
     7441 tgcaactcca ttggaaagaa gtaggccctg tacccttgtc caggacaaag gatatctggg
     7501 agacctatca gcactgcgtt gaaaaattct acgattacgt caaaataaat aacgagttgc
     7561 gcgacctgga cttcaagcgc aacctggaga tcaaggaaaa gctttgtgaa aaggccgagg
     7621 cgcttgttga agaacccaat gtggtttcgg catttcacaa attgcagaaa ttgcatgagg
     7681 aatggaggga aacaggaccc attgcgaggg aattgcgtga atccatgtgg gaacgcttca
     7741 aaataagcac aacggccatc aataagcgcc atcaggatta ttttgaagaa cagaagcagc
     7801 agcagagaaa aaacctggaa gcaaaactgg ctttttgtga aaaagccgaa gccatagcta
     7861 aggaattgac agatgacgat gtgaactgga accagaaagc caaagagctt gaagcacttc
     7921 aaaaggaatg gagaaccatt ggctttgcca cgaaaaagga aaatcaaaag gtttatgacc
     7981 gttttcgtaa tgcatgtgat cagttttacg gtgaaaaacg cggattttat tccaagttca
     8041 aaaaagaaat gcaggaaaac ctggcactca aaatcgcatt atgtgagcag gccgaagcaa
     8101 tcaatatgag cgacgactgg cgtcgtacca ctgaccagct tatccatttg caaaaacaat
     8161 ggaaggacat aggcccggtt cccaggaaac aagccgatgc catatggaaa cgtttccgct
     8221 ctgcctgtga cgtgtttttt acccggaaag ctgaacattt ttccaagatt gataccgtat
     8281 acctcgaaaa cctggctgcc aaggaagccc tgcttaaaga actcgataat tttgatcagt
     8341 cagatgttaa agatctgcaa gctgcattac gagacttcca gaatcgttgg aaccagatag
     8401 gctttgttcc cggaaaagaa aaagaaagga ttaataatga atacaggaac ctgatcaatc
     8461 tgcatttcaa aaatcttaac ccgggctttt ccgaaaagcg tacccagtac cgtacgggca
     8521 ggggacgttc agacgaattc cgaagcggaa gggataagct gattcaacgt ttccgccagt
     8581 tggaatccga gatctctgtg tgggaaaaca atatgggatt ttttgccaag tcaatgaatg
     8641 cagataagat cattgcagac acctgccgga agatagagac agcaaaacag gaactggcag
     8701 agttggaaag caagatcaaa caattagaag ataatgaata agaatacagt atgggctatt
     8761 ttgcttatcg gggcaattct ggtagggttc agtctttata actccagtgt tgccaaaaaa
     8821 cagcaaagag aacaattcgt cagggattcc attgcccgtg aagtgaccat tgaagccatc
     8881 aggctggagg aacagaataa acccgagaat ctcccacagg cagaggatca gatgatccag
     8941 ccgtctgtca cggctcccgg acaaaggtac accaatcctt tcctgaacca gtcacttgaa
     9001 ggagaagaat cattttatat acttgaaaac gataagttaa ggatttcctt caccaataaa
     9061 ggcggtcagc cttacagctt acagatcaag aatttcaaaa cgtacgctgg ggacagtctt
     9121 atatttttca gcgggccctc caataaactg gcgcttgatt tgtacgcggg agaacagatc
     9181 aatacgacag attttttctt tgttccccat ttctcagctg ataacaacag cctgactttc
     9241 tcgcttcatt tcgatgagca atcatacatc gaatacatct atgtcctgga aaaggattca
     9301 cacctgctaa acatggatat caagctgaca ggcatggacc gttacattcc aaggaacgtg
     9361 acccagcttg acctgaccct gaatctggac attcgccatc tggaaaaagg ttttgataac
     9421 gaaaagaatt attccaccat atcctacaaa tatcccaaca ctacggatgt tgaaaaatta
     9481 ggacaaagaa aagcagaagc cagtgaatca atcaaaacac gtgtagaatg ggtagctttt
     9541 caacagcaat ttttctcggc catattgata tccgatcaaa acttcaacag cggggatctt
     9601 tcattccggt tctacgatgc agccaacccg gacaccctgt tgataaattg ccgtgcttat
     9661 atgcagattc cattccaggc atctgcagaa caaagtgttc cgctacgctt ctattttggg
     9721 cccaatgggt tttattcact caaggctttt gatcgttctt ttgaaaagat tattcccctg
     9781 ggtggttttc tgattagttg gattagccgc ttcctgatca ttcccacttt caatttcctg
     9841 aaccgcttta tcagcaacta tgggctcatt atcctaataa tgaccatatt cataagactc
     9901 atcatctctc cgttcacata caggtcgcag atgtcctcgg caaaaatgcg tgttctgaaa
     9961 ccagaggtcg acaagatcaa cgccaagtat cccaagaagg aagatgccat gaagaagcaa
    10021 caggaaacta tggctttgta taagaaaaca ggtgtcagta tgatgggtgg atgctggccc
    10081 atgttgtttc agatgcccat cctttttgcc atgttccgtt ttttcccggg ctctattgaa
    10141 ttacgacagc aggggtttct atgggccgat gacctgagtg gttatgattc aatcctgaac
    10201 ctgcccttca agattcctct ttatggagat catataagcc tttttgcact gttgatggct
    10261 ctttccatgt ttttctattc caagatgaac atgaaagagc agtcttccgg ccagcaaatg
    10321 cccggcatgc aggggatgat gctttatttc atgcccctta tgatgcttgt cgtctgtaac
    10381 aacctttccg ctgccctgag ttattactac ctgctttcga acctgacaac catgttacag
    10441 acatggatca tgcaaaaatt cgtcgtagac gaagcaaaaa tataccagca actgaaagat
    10501 aaagcagata aagcaccttc gactcccaag aaatccaagt tccagcaaag actggaagaa
    10561 atgcaacgtc agcagcaaca gcagatgaaa aagaggagat gatcgcccgg aatttacatc
    10621 ggttgtatca gcatatcccc aaaagcgtaa cactggtagc tgtctcaaaa tttcaacccg
    10681 taccctttat tcttgaagct tatggctcag gccaacgttg ctttggtgaa agcaggtccg
    10741 gcgaaatgct tcagaaaatc ccgcttttac ctcaggacat tcagtggcat tttgtcgggc
    10801 acctgcagac gaataaagta aaagaagtgg taggaaaggc ttctctgatc cagtccgttg
    10861 acagtgtcag gctcctgaat gccatacagc agcaggctca tcagctgggg attgtacagg
    10921 atgtgctttt gcaggttcat atagcagtgg aagagacaaa acatggtttt cttccttcag
    10981 aactgcagca ggtttgctca ttatccctgc ctgcagtgag gattcgcgga cttatgggaa
    11041 tggccacata cacagaagat aatgaccgga taagaagtga attcaagaca ctccggcaac
    11101 tttttgacac attgcggtca ggtccctttg ccggtaagga tgattttacc cattgttcca
    11161 tgggtatgtc cggcgattat cagattgccc tggaagaagg cagcaccatg gtaaggattg
    11221 gcagtgctat atttccatca cgccctcaaa gaccctgacg gcaccccctg taagaaacac
    11281 ctcggtgaaa gcattgtcat tcttctgaaa ggatacctcc aggtttcctc cctttgtctg
    11341 tatatgccaa tgattcagtc ctgtacctgt gtgtacagag gcagctatgg cagccgctgt
    11401 cgatcctgtc ccacaggccc atgtctcatc ctcaacgcct ctttcaaaag tcctgacaaa
    11461 cagacatcct gtatccagaa tttcaacaaa gttcacattg attccttttt ttccaaaatc
    11521 tttatggtgt ctccagtaag gaccctcagt ggcaatatct acggccttta catcgggaac
    11581 aaaacagaca agatgcatgg aaccggtatc tactatataa tagtccttct ggtagggttt
    11641 tatgccatca acattgttaa gttgcagctt taccctggag taattatcct tgttttcgat
    11701 cacgtacgcc tggtgcgcac catcaacagc tgtgaatgag tgcttgttta ttcccaggtg
    11761 attggcaaat gccaccaggc atcgcccccc gtttccgcac atggtgccac ttgtcccgtc
    11821 taaattgaaa aaagccatgg aataattcca tggagcatca gcccgctcaa ggagcatcag
    11881 tccgtcggcg cctatgccaa acctgcgatg gcataaagcg ctgatctgaa agcttgttag
    11941 ttcgtattgg cctgacaagt tgtctataat gataaaatca ttcccggctc cgtggtattt
    12001 acaaaatggc agtttcataa aattggtatg gattttgaaa tatttcttat caaaaatagt
    12061 taaattaaat cagatacagg tatgaaaaag agaattggaa ttattgttat agcgactgct
    12121 attgtaagcg ttggagccac aatcctggtt cacagcctta cagaaaatga aagcaaagtt
    12181 gaaaaagttt accttacttc agcacaggaa cctgtaattc ataatgtttc cctgccagaa
    12241 gtgcaatatc cggattttac atatgctgca gaaaccggag ttaaggcagt agttcacgta
    12301 aaagtggtca agcacggaca acaggcccag ggcccccaaa gtctttttga ttttttcttt
    12361 ggatacggct ccccttcgcc acaaccgcgt cccatagtag gagcgggctc cggtgtaatc
    12421 cttacttctg atggatacat agttacagca acacatgtta tagaaggagc ggatgatgta
    12481 cttgttaccc tggatgacaa acgccagttt gaaggcaagg tcataggaaa agatcccgcc
    12541 accgatattg ctcttctcaa gatagaagcc actaacctgc catatctgca gttcgggaac
    12601 tcggatgatt tacgtttagg ccagtgggta attgccatag gaaatcctta tgatttgcgc
    12661 tcaaccataa cagccgggat tgtcagtgct aaaggccgca gtttgccctc ctacaatggg
    12721 gaattcaaaa tagaggcttt tatccagaca gatgctgccg tgaatcccgg taattccggt
    12781 ggtgccttgg ttaacgccaa aggtgaattg gtaggtatca ataccgccat tgctacccgt
    12841 acaggctcct atagcggtta ttcctttgct gttccaagca gcattacaaa gaaggtggtg
    12901 gatgatcttc gcaaatacgg tatcgtccag cgtgcactgc tgggtataac catgcaggat
    12961 atcacaggag accttgccgc agaaaagaat atcaaggaca tccgtggggt gttcatacat
    13021 gaattggtga cagatggagc tgcagagaaa gcaggtgtaa aacccggtga catattgctt
    13081 caggtggaag gagtacctgt taattctgga acggcagtcc aggaacagat caaccgcttc
    13141 agtcccggtg acaagattga cttgttatta ctcaggggaa ataaagaggt taaagtgacc
    13201 gcaaccttac agaaccagtc aggagatact gagcttatca aggaagggac tggcgatctc
    13261 acctttttcc tgggggcaca actgcgccct gccacaaaag agatcaggga ccaactcaaa
    13321 atacgcgcag gaatagaagt cgtttcaata gaggaaggta aattaaggga cgccggaatc
    13381 cgtaaaggat ttgtaattac acacattaac cagaaacagg tagggactgt acagcagctt
    13441 gcccttgcca tacaaaccgc acagcggggg gtgctgattg aggggaggta tcccgacgga
    13501 tctgtatatt attacgcttt aagcaattaa tttgccattt tattgatgta atttgaaact
    13561 taaactgtcc ttttacgtat aaaagggcag tttatatttt tttacggatg agacaattaa
    13621 aaatcacgaa atctatcacg aaccgggaaa gtgcttccct ggacaagtat ttacaggaaa
    13681 ttggcaggga agatctgata tcggtagaag aagaagtgga gctggcgcag cggatcaaaa
    13741 agggcgatca ggaagcactt gagaaattga cgagggcgaa tctcagattc gttgtttcag
    13801 tagctaaaca ataccagaat cagggtctta gccttcctga cctgatcaac gaagggaatc
    13861 tgggattaat aaaggctgct gaaaagtttg atgaaacaag gggttttaaa ttcatatcat
    13921 atgccgtatg gtggatcagg caatccatac ttcaggcttt ggccgaacaa agcaggattg
    13981 tcaggctgcc tttgaaccag gttggatctt taaacaagat aaacagggct ttgtcaaa
//
LOCUS       DAOSOF010000041        14001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4378_length_14001_cov_6.927076, whole genome shotgun sequence.
ACCESSION   DAOSOF010000041 DAOSOF010000000
VERSION     DAOSOF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4378_length_14001_cov_6.927076"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1289
                     /locus_tag="PLM68_03705"
     CDS             114..1289
                     /locus_tag="PLM68_03705"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOT54504.1"
                     /translation="MSTVLTKQRYVALDVLRGMTIAGMILVNNPGTWSQIYPPLRHAE
                     WFGCTPTDLVFPFFLFVVGTAMAFSFARFFDGTASMKSGYAKLYKRAFLIFLVGLLLN
                     AFPFFPVRPDPELTFWENLGQFYSRLRILGVLQRIAMAYALGGTLALWLKKPKRIAVA
                     FAATLLVHWALLWIFGGEDPYSRETNFARTLDLFLLGPQHLYKGYGLPFDPEGVLGMI
                     SGAGTVLMGYLAGVNIRKSVEKIQAVGSLYTWGLVSLGAGMVWSIWLPIGKPLWTGSY
                     VLYAGGWTLLMLAFFIYLIDIKGREKWFLPFKALGLNPLFAFVLSGLFAKIFGSIIRW
                     QTADGSTTTPLRWLYTNVFAAVLGDNPAGSVAYAIFYVAIFTLMAMALYKKKIIIKL"
     gene            1308..3791
                     /locus_tag="PLM68_03710"
     CDS             1308..3791
                     /locus_tag="PLM68_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT54505.1"
                     /translation="MRSKQMLMVCLVALASISLQAQPQRGATREKANYELASRFSRKK
                     TDKMVFSTSVRPIWFKTSDLFWYEYKTSEGTNWYVADPAKATQQELFDKVKLATELTQ
                     ITKDPFDAQNLPLKELRLKDDDTFTFYIQGTEMVEKKKKDNEENKNDKPGAKNGKEPR
                     LFFFEYAWKTRNLTWLKDKEKEDPVPRWANISPDKKYVVFSRNFDLYWMDWENFEKAR
                     KDEKDSTIVEHRLTTTGTREFAFGSGSEDLYADSTELNKRTGLYGLLWSPDSRHFALV
                     RSDERKVKDLFVVDVLAQPRPKLERYKYEMPGEENIPQQSLVVFDMESKTWKTIETAA
                     FKDQSLDLGYKPMEKRKMSDEFRHSEWLGDASGFYFHRTSRDLKRIDLCHAEIDKDTA
                     ITLIEERLNTYVETRPLFLVNNGKELIHWSEKTGWGHLYLYDNQGHEKNAITSGPWHV
                     EQILGVDETTRTLYFTACGREKGLDPYFEHIYSVKLDGTQLRNLTPGDFHHTADISDS
                     RKALVINRSRVDVAPVSELFDNTGKKLLALQETDLRLLFEAGYKFPERIKVKAADGVT
                     DLYGVMYKPFDFDPERKYPIITYVYPGPQVEGPGQSWSRSMDRLDRLAQVGFIVITVG
                     NRGGHPNRSKWYHNFGYGNLRDYGLEDQKYAIEQLAARHPFIDVSRVGIHGHSGGGFM
                     STAAILQYPHFFKVAVSCAGNHENNIYNRWWSEKHHGVVEEISAKGDTTFKYSIGTNS
                     QLAKNLEGRLLLIHGDIDNNVHPGNTLRVANALIRANKRFDMLILPGQRHGFGDMTEY
                     FFWRMADYFSQYLLGDYQNTVDIPQMYND"
     gene            complement(3947..>4443)
                     /locus_tag="PLM68_03715"
     CDS             complement(3947..>4443)
                     /locus_tag="PLM68_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54506.1"
                     /translation="NIKRMNMDTLRDFFEEEQRRHREEAAGMPPLGMRVTELIAIQEA
                     ARKKTHRIWWLTGGAGFLMVAVFAAMFFRDQLAEAFRWVETVVSQTTGEVSGFIENIK
                     YTVISRASGISAPAGEGFIEKATAEISGWPVQIWYVVLLVAGVGLLLGADHLVRRRRS
                     AAHS"
     assembly_gap    4444..4543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4544..4953)
                     /locus_tag="PLM68_03720"
     CDS             complement(<4544..4953)
                     /locus_tag="PLM68_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54507.1"
                     /translation="MTQDVTAIQKVLDGDVRAYAALVNQYQSRVFVLVRSVCRSAEDA
                     QEVTQDVFLKVYQQLHTFKGNSSFSTWLYRIAYNTAISSIRKTHSLERRTQDYAKDVL
                     HTTDQAEEETDDPRVAQQDKLRSLLETLPPQDRML"
     gene            5089..5478
                     /locus_tag="PLM68_03725"
     CDS             5089..5478
                     /locus_tag="PLM68_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54508.1"
                     /translation="MNLTAVLIVATVFYFIYAIWNRMATRKERLRFMETLASMSPDAL
                     SAYKEINPQWDLNVKKDPKRNIRPACLLIGLGIGLLIVWLGFTAWYPGDGIVSLHDGI
                     NGFLMLASPLLFGGIGLLVAYFIERKN"
     gene            5505..6620
                     /gene="mnmH"
                     /locus_tag="PLM68_03730"
     CDS             5505..6620
                     /gene="mnmH"
                     /locus_tag="PLM68_03730"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOT54509.1"
                     /translation="MTEPISVEEFLKRSVTTPVADVRTPAEYEKGHIPRAVLFPLFSD
                     AERAEVGTLYVQQGRTAAVLKGLEVTGPRLEAMARRGLEIARGGPGTATKVQRSTAKE
                     LVGTPGKPATSGTPAAHEASGPAGESATASVGSLLLYCWRGGMRSASVAWLMETVGIR
                     CYTLEKGYKAYRNHILGFFEHLPHPLKVLGGLTGSGKTERLRQMAGAGEQVIDLEGLA
                     GHKGSAFGNLGLPPQPSTEHFQNLLYDAFSRLDPERPVWLEDESRNIGRCSLPKGLWE
                     KMQQAGFYLLPSSLEERVERLMKEYAQFDPALLENAILKIEKRLGYDLCKKATEACQS
                     GNIRTALQICLAYYDKAYNYQLEIRKKNYGTGTFDTI"
     gene            6607..7875
                     /gene="selD"
                     /locus_tag="PLM68_03735"
     CDS             6607..7875
                     /gene="selD"
                     /locus_tag="PLM68_03735"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOT54510.1"
                     /translation="MTQFSPGAGCGCKISPAQLRQILQESLPGRPKKDLESKGNSARE
                     QGANVAGLDKGPGNQRAAEADLSKGAIEFPQLLVGYESCDDAAVYDIGSGKAVISTTD
                     FFTPIVDDPYDFGRIAATNAISDIYAMGGTPLMAISILGWPLEKLPAAVAGKVIEGAR
                     TICSQAGIPLAGGHSISISEPVFGLAVTGIIDKELVRRNNSVQKGDYLFLTKPLGIGL
                     ISTGIKRGIALQEHMETALRLMTRLNAEGPRLALLPGVHALTDVTGFGLLGHCLEMVE
                     GSGLTARIRVDDVPLIPGIDFYVKENCIPGGTYRNYDSYGHRLAFSAADNTVIVGIPE
                     SGTRQDATLPDAAQKTVTAGFLKAAKLPDTAQKTESAGIPEGITLPDPVKLVLCDPQT
                     SGGLLIAAHPDSRIVLEKEYGAVCVGEIVI"
     gene            7977..9161
                     /locus_tag="PLM68_03740"
     CDS             7977..9161
                     /locus_tag="PLM68_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54511.1"
                     /translation="MYERTYLKQVKSRIEEPRQFIQVILGPRQVGKTTMISQLLSQLS
                     ISYVYESADAITATNPVWLMQVWETARLKLKASGASEYLLVIDEIQKIDNWSEVIRQQ
                     WDKDTREKVNIKVVLLGSSRLLIQKGLTESLAGRFETFYLGHWSFVEMEAAFGWSVEQ
                     YVYFGGYPGSASLVNEEERWKSYIKDALIETSISKDILMLTRVDKPALLKRLFELGCL
                     YSGQILSYTKIIGQLQDAGNTTTLANYLKLLSDSGLLGGLGKYAGSVIRQRSSSPKFQ
                     VYNNALLTSQDNESYSTAIVNPKLWGKLVESSVGAHLLNHSVSERYNLHYWREGNHEI
                     DFILEKGDKVIGLEVKSGTSTDNKGMGVFNEKFHPDKILLVGTGGIPYEEFLKINPKE
                     LF"
     gene            complement(9198..10481)
                     /locus_tag="PLM68_03745"
     CDS             complement(9198..10481)
                     /locus_tag="PLM68_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT54512.1"
                     /translation="METSVVYKPRIADGMLQEQLEASGVVLIQGPKWCGKTTTAEQQA
                     KSVLYMDAPKTRKANLLLSESDPEILFQGETPRLIDEWQLAPKLWDAARFEVSRRRLP
                     GQLIFTGSAVPPDLTELTHSGTGRFAWLTMRPMSLWESGDSNGSVSLNSLFQGNMTPV
                     VSRDISLKELAFLICRGGWPGSLSLTPKAALRQAANYVDSVANSDISRVDDVSRNAEF
                     TRRLLRSYARHQGAQVSVNTIYDDLEKNRMGSMSEDTIASYIVALKKIFVIEDMPAWS
                     PNLRSKTAIRTSDTRYFVDSSIAAAALGLGPEDLIGDLNTFGLLFETMVVRDLRVYAD
                     ALDGQVYHYRDKNGLECDAVVHLRNGCFGLIEIKLGSDRLIESGAHTLKRLSDKIDTT
                     RMKSASFLMVITGQGQYAYRREDGVYVVPIGCFKD"
     gene            complement(10846..10995)
                     /locus_tag="PLM68_03750"
                     /pseudo
     CDS             complement(10846..10995)
                     /locus_tag="PLM68_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(11015..11434)
                     /gene="tsaE"
                     /locus_tag="PLM68_03755"
     CDS             complement(11015..11434)
                     /gene="tsaE"
                     /locus_tag="PLM68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT54513.1"
                     /translation="MELIFSLENIRQAAGALLQHTGKEGVVAFYGQMGAGKTTFIKAL
                     CQVLGTEDWVNSPSFALVNEYLSSEGIPIYHFDFFRIKNIQEALDMGCTEYFETPGTL
                     SLVEWPELITPLLPPDTWHATLEVLPSGERKLTVSRG"
     gene            complement(11453..11650)
                     /locus_tag="PLM68_03760"
     CDS             complement(11453..11650)
                     /locus_tag="PLM68_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT54514.1"
                     /translation="MKNNIRVERAIKKITQQDLADLCGVSRQTINAIETERFVPSTVL
                     ALKIARVFEKTVNDLFILEDE"
     gene            complement(11640..12119)
                     /locus_tag="PLM68_03765"
     CDS             complement(11640..12119)
                     /locus_tag="PLM68_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54515.1"
                     /translation="MKRKRILLPHKFQVIGWGMFSLAVMLLLFILLRYEWNTSHENTF
                     LPLVDQYSRYVTSLLLVLAYGSAFFVAFSEEKQEDELVREIRFSSVTIVAAVLFALMV
                     LFSLLAALSQGFRLNFFTERSYYVMHWISNILFAFFIYVIVFRVRLFIIKRRLKNEE"
     gene            12306..13451
                     /locus_tag="PLM68_03770"
     CDS             12306..13451
                     /locus_tag="PLM68_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54516.1"
                     /translation="MEQKILLRKLGPIIEKAMVPQRVVLIFGARRVGKTILLESILKQ
                     VKGNVMLLNGDDADTLRLLEERSISNYRRLLNGVDVLAIDEAQQIPEIGHKLKLIVDT
                     LQDIRVIATGSSSFDLLNKSGEPLVGRARQFLLTPFSQLELSQEENLLETRRALPDRL
                     IYGAYPDVVKMGNDTDRQNYLEQMAEGYLLKDILELDGIRNSAGLVNLLKLIAHQVGN
                     EVSYDELGKQTGLSRNTVIRYLDLLSKVYIIYSLSGYSGNMRKEVTKSRKWYFYDNGI
                     RNVLTGDFRPEALRSDMGALWENYMVSERVKNSLNKDGLEKFYFWRTYDKQEIDLIET
                     SPDGSVRAFEFKWGNKKPRVPKSFATSYPHATFQAVNPDNYHDFLQL"
     gene            complement(13825..>14001)
                     /locus_tag="PLM68_03775"
     CDS             complement(13825..>14001)
                     /locus_tag="PLM68_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HOT54517.1"
                     /translation="EVCALGPACRTFLRQALERLHLSARAHHRILRLARTIADLEGSP
                     AIEIHHLAEAIQYR"
BASE COUNT         3561 a         3478 c         3496 g         3366 t
ORIGIN      
        1 agggtgcaca cccgttgacc tgggaccttc cgtgctcaga acggaaactg ccgccttagg
       61 ggctgtcttt cttgttgcat tcaaatcatt atatttgtaa aaactatctt ataatgtcta
      121 ctgtattgac aaaacaaagg tatgtggcat tggacgtgct gcggggcatg accattgccg
      181 gcatgatcct ggtgaacaac cccgggacct ggagtcagat ctatcccccg ctgcgtcatg
      241 ccgaatggtt tggctgcacc cctaccgacc tggtgttccc ctttttcctg tttgtggtag
      301 gcacggccat ggcattctct tttgcacggt tttttgacgg aacggcttcc atgaaaagcg
      361 gttatgcaaa actgtataaa cgggcttttc tcatcttttt ggtaggcctt ctgctgaatg
      421 cttttccttt ctttcccgta agacccgatc cggaactgac cttctgggag aacctgggac
      481 agttttattc ccggctcagg atactgggtg tgctgcaacg cattgccatg gcctacgcac
      541 tgggaggcac cctggccctg tggttgaaaa agccgaagcg gattgcggtg gcttttgccg
      601 cgaccttact ggtccactgg gcattgcttt ggatttttgg cggtgaagac ccctattccc
      661 gggaaacgaa tttcgcgcgc accctggacc tgttcctgct gggaccgcaa catctgtaca
      721 aaggctacgg actgcccttt gacccggaag gcgtgctggg catgatctcc ggagccggca
      781 cggtgctcat gggctacctg gccggggtga acatccgcaa gagcgtggaa aagatccagg
      841 cagtgggttc gctgtacacc tggggactgg tgagcctggg agccggcatg gtgtggagca
      901 tatggctccc cataggcaag ccgctctgga cgggatcgta tgtattgtat gcaggcggct
      961 ggaccctgct catgctggcc ttcttcattt accttataga cataaagggt cgcgaaaagt
     1021 ggttccttcc tttcaaggcg ctgggactga acccgttgtt tgcctttgtg ctttccgggc
     1081 tttttgccaa gatcttcggg agcattatcc gctggcaaac ggccgacggc tcaaccacca
     1141 cgccccttcg ctggctttat accaatgtgt ttgcagccgt cctgggagac aatccggccg
     1201 gctcggtggc ctatgccatc ttttacgtgg ccatattcac ccttatggcc atggccctgt
     1261 ataaaaagaa aatcatcatc aaactctaaa tcctaaaatc tataatcatg agaagtaaac
     1321 aaatgttgat ggtctgcctg gttgcccttg cttccatctc cctacaggca caaccgcagc
     1381 ggggcgcaac gcgtgaaaaa gccaattacg agctggcttc ccgtttttcc cgcaaaaaaa
     1441 cagacaaaat ggtcttttcc accagtgtcc ggcccatttg gttcaagaca tcggacctgt
     1501 tctggtacga atacaagact tcggaaggca ccaactggta cgtggccgac ccggccaaag
     1561 ccacccagca ggaacttttc gacaaagtga agctggccac ggaactcacg cagatcacaa
     1621 aagatccctt cgatgcccag aacctgcccc tgaaagagct taggctcaag gatgacgaca
     1681 ccttcacctt ctacatccag ggcacagaga tggtggaaaa aaagaagaaa gacaacgaag
     1741 agaataaaaa cgacaaaccg ggtgcaaaga acggcaagga accccgcctg ttctttttcg
     1801 agtacgcctg gaaaacccgc aacctcacct ggctgaagga caaggaaaaa gaagatcccg
     1861 tcccccgctg ggccaacatt tcgcccgata aaaaatacgt tgtcttttcc cggaacttcg
     1921 acctctactg gatggattgg gaaaactttg aaaaagcacg caaggacgaa aaggactcca
     1981 ccattgtgga acaccgcctg accaccaccg gaacccgtga attcgccttc gggtccggat
     2041 cggaagattt atatgccgat tccaccgagc tcaacaagcg caccggcctg tatggcctgc
     2101 tgtggagccc cgattcacgc cattttgcgc tggtccgctc cgatgaaaga aaagtaaaag
     2161 acctgtttgt tgtagatgta ctggcccagc cccggcccaa actggagcgt tacaaatacg
     2221 aaatgcccgg agaagagaac atcccgcaac agtcactggt ggtgttcgac atggaaagca
     2281 aaacctggaa aaccatagaa acggccgctt tcaaggacca gtcactggac cttggctata
     2341 aacccatgga aaaaaggaaa atgtcggacg aattccgcca ctccgaatgg ctgggtgatg
     2401 cctcgggctt ttacttccac cgcaccagca gggacctcaa gcgcattgac ctgtgccacg
     2461 cagaaatcga taaagatacg gccataaccc ttatcgagga acggctgaac acctacgtgg
     2521 aaacgcgtcc cctgttcctg gtaaacaatg ggaaagagtt gatccactgg tccgagaaga
     2581 ccgggtgggg gcatctttac ctgtatgaca accagggcca tgaaaagaac gccatcacct
     2641 ccggcccctg gcacgtggaa cagatcctgg gcgtagacga gacaacccgc acactctatt
     2701 tcaccgcctg cggcagggaa aagggcctgg acccctattt cgaacacata tattccgtaa
     2761 agctggacgg cacccagctg cgcaacctca ccccgggcga tttccaccac acggcagata
     2821 taagcgacag ccgcaaggca ctagtcatta accgctcccg cgtggacgtg gcccccgttt
     2881 ccgagttatt tgacaatacc ggcaagaaac tcctggcgct gcaggaaacc gacctgagac
     2941 tgctatttga agccggatac aaattcccgg aacgcatcaa ggtgaaagcc gccgacgggg
     3001 tcaccgacct gtacggggta atgtacaagc cttttgattt tgaccctgaa cgcaaatacc
     3061 ccatcatcac atacgtatat cccggcccgc aggtagaagg ccccggccag agctggagcc
     3121 gctccatgga ccgtctggac cgcctggccc aggtgggatt cattgttatc acggtgggca
     3181 accgcggagg ccatcccaac cgctccaaat ggtaccataa ctttggttac ggcaacctgc
     3241 gcgattacgg gctggaagac cagaaatacg ccatagagca gctggccgcc cgccatcctt
     3301 tcatagatgt ttcccgcgtg ggcattcacg gccactcggg cggcggattc atgtccacgg
     3361 ccgccatcct gcagtacccg cacttcttca aggttgcagt ttcctgcgcc ggaaaccacg
     3421 aaaacaacat ctacaaccgc tggtggagcg aaaagcatca cggcgtggtg gaggaaatct
     3481 cggcaaaggg cgataccacc ttcaaataca gcataggcac caattcacaa ttggccaaga
     3541 acctggaagg aaggctgctg ctcatacacg gcgacattga caacaacgtg cacccgggga
     3601 ataccctgcg cgtggccaat gccctgatcc gggccaacaa gcggtttgac atgcttatcc
     3661 tgcccgggca gcgccacggt tttggcgaca tgactgaata cttcttctgg cgtatggccg
     3721 attacttttc acagtacctg ctgggagact accaaaatac ggtggatata ccgcagatgt
     3781 acaatgacta ggctgcttgt ccggggctcg aagtggagtg ccccgcaggg ccgggcgact
     3841 gaaagtgccg cgtacacttg atgtataaaa gtgtataaca agtagttaag aaaagagctc
     3901 tccgggagag ctcttttctt atattgctga tggtcaacgg tatgaatcaa gagtgcgcgg
     3961 cactccggcg gcggcgcacc aggtgatccg ctcccaggag caggcccacc ccggcaacca
     4021 gcaacacaac ataccagatc tgaacgggcc acccggaaat ttccgcagtg gccttttcta
     4081 tgaacccttc cccggcaggg gcgcttatgc cggaagcccg ggaaatcacc gtatacttga
     4141 tgttctcaat gaaaccgctg acttccccgg ttgtctggct gactacggtt tccacccaac
     4201 ggaaagcttc tgccagttga tcccggaaaa acattgccgc aaatacggca accatcagaa
     4261 agccggcgcc cccggtcagc caccagatcc ggtgtgtttt tttccgggcg gcttcctgta
     4321 tggctatcag ttccgttaca cgcattccca gcgggggcat cccggcagct tcttcccggt
     4381 gcctgcgttg ttcttcttca aagaaatccc ttaatgtatc catgttcatt ctttttatat
     4441 tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcatcc tgtcttgcgg
     4561 agggagggtt tccagcaggg agcgcagctt gtcctgctgt gcaacgcgcg gatcgtctgt
     4621 ttcttcctct gcctggtctg ttgtgtggag cacatccttt gcgtagtctt gtgtgcgcct
     4681 ttccaggcta tgtgtcttgc gtatgctgct tatggccgta ttgtaggcga ttcggtacaa
     4741 ccaggtgctg aacgaggagt tgcctttaaa ggtatgaagc tgctggtaca ccttaaggaa
     4801 aacgtcctgg gtcacttcct gggcgtcctc tgccgagcgg cacacgctcc gtaccaacac
     4861 aaaaaccctg ctctggtatt ggtttaccag ggctgcataa gctcgtacat ctccgtccag
     4921 taccttttgt attgcagtaa cgtcctgtgt caagacactt tttttctttt tttcttcgcc
     4981 tttttgacgc aaaaacagaa aaaacgttac atctcttgta accggaatgg aattgttacg
     5041 tcaaagaagt gaaagtaaaa aaatgtttca cgctaaatta ttaaaaaaat gaatcttact
     5101 gctgttttaa tcgttgcgac cgtattttat ttcatttacg ccatctggaa ccgcatggcc
     5161 accaggaaag aacgtttgcg tttcatggaa acactggcct cgatgagccc cgatgcactg
     5221 agcgcttata aagaaatcaa tccccaatgg gacctgaatg ttaagaaaga tcccaaaagg
     5281 aacatcaggc ctgcctgcct gctcatagga ctgggcatcg ggttgctgat cgtatggctg
     5341 ggctttactg cctggtatcc cggagatgga atcgtatccc tgcatgatgg gataaatggt
     5401 ttcctcatgc tggccagccc gctgctgttc ggaggtatcg gcctgttggt ggcttacttt
     5461 attgagcgga agaattaaaa aaggtttttt catacctttg cggtatgaca gaaccgattt
     5521 ctgttgaaga gtttctaaaa agatcggtca ccacgccggt agccgatgtg agaacgcccg
     5581 cagaatatga gaaaggtcat attccgcggg cagttctttt tccccttttc agtgatgcgg
     5641 aaagggccga ggtgggcacg ctgtacgtac agcaggggcg caccgccgcc gtgctcaaag
     5701 ggctggaggt gaccgggcca cggctggaag ccatggcccg ccgcgggctt gaaatcgccc
     5761 gcgggggccc ggggactgct accaaagtgc agcgctccac tgccaaagag cttgttggca
     5821 ctccagggaa accagccact tcaggaacgc cggctgctca tgaagcatct ggccctgcag
     5881 gagaatctgc tactgcgtcc gtgggttctt tgctgctata ctgctggcgc ggggggatgc
     5941 gctccgcctc ggtcgcctgg ctcatggaaa cggtgggcat ccggtgctac accctggaaa
     6001 aggggtacaa agcgtaccgc aaccacatcc tcggcttttt tgaacacctg ccccatcccc
     6061 tgaaagtgct gggcgggttg accggcagcg gcaaaacgga gcgtttgcgt caaatggccg
     6121 gggcagggga acaggtcatt gacctggaag gactggcggg gcacaaaggc tcggcgttcg
     6181 gcaacctggg gcttcccccg cagccatcca ccgaacattt ccagaatctg ctctatgatg
     6241 cgttttcgag gctggacccg gagcggcccg tgtggctgga agacgaaagc cgcaacattg
     6301 gccggtgcag ccttcccaag ggcctgtggg aaaaaatgca gcaggccggg ttctatttgc
     6361 tgccgtcttc cctggaagag cgggtggagc gcctgatgaa ggaatatgcg cagtttgacc
     6421 cggcgctgct ggaaaatgcc atcctgaaaa ttgaaaagcg cctgggatac gatctgtgca
     6481 aaaaagcgac agaggcctgt cagagcggga acatcagaac agccctgcaa atctgcctgg
     6541 cttattacga caaagcatac aattaccaat tggaaatacg caagaagaac tatggaaccg
     6601 gtacgtttga cacaatttag cccgggagcc ggatgcggct gcaaaatctc ccccgcccaa
     6661 ctccgccaga tactgcagga aagcctgccc gggaggccga aaaaggacct ggaaagcaag
     6721 ggcaactctg cacgtgagca gggtgccaat gtggcgggcc tggataaagg tcctggcaac
     6781 caacgtgccg cagaagcgga ccttagcaaa ggtgccattg agttcccaca attgctggtg
     6841 gggtacgagt cctgcgacga tgcggctgta tacgacattg gcagcgggaa agccgtcatc
     6901 agcaccaccg atttctttac ccccattgtg gacgacccgt atgattttgg acggatcgct
     6961 gccactaacg ctatctcgga catttacgcc atgggtggaa cgcccctgat ggccatctcc
     7021 atcctgggct ggccgctgga gaaactgccg gctgcggtgg cggggaaggt gattgaagga
     7081 gcacgtacca tttgctcgca ggccgggatc cccttggccg gtggacacag catcagcata
     7141 tcggaacctg ttttcggcct ggccgtgacg ggcatcattg acaaggaatt ggttcgccgg
     7201 aacaacagcg ttcagaaagg tgattacctg ttccttacaa agcccctggg cattggcctc
     7261 ataagcaccg ggataaaaag gggaatcgcc ctgcaagaac atatggaaac ggccctgcgc
     7321 ctgatgaccc ggttgaatgc agaaggcccc cggctggcgc tgctgcccgg cgtgcatgcc
     7381 ctgaccgatg tcaccggctt cggcctgttg ggccattgcc tggaaatggt ggaaggctcc
     7441 ggccttaccg cccggatccg ggtggatgac gttcccctca tccccggcat tgacttctat
     7501 gtaaaggaaa actgcattcc cggcggaacc tacaggaatt acgatagtta cggtcaccgc
     7561 ctggcatttt cagctgcgga caataccgtg atcgtgggaa tcccggaaag cggcacccgg
     7621 caagacgcca cactacccga tgctgctcaa aagactgtga ctgcagggtt tttgaaagcc
     7681 gccaaactgc ccgatactgc tcaaaagact gaatctgcag ggatccctga aggcatcacc
     7741 ctgcccgacc cggtgaaact cgtgctctgc gacccccaga ccagcggcgg actgctcatc
     7801 gccgctcacc cggatagccg gatagttctt gaaaaagaat acggggcggt ctgtgttggg
     7861 gagatcgtga tttgatttat gtttcgaaat ccgtggcacc ggaacaatat tcaatttttt
     7921 tggcaatcca gatattttga gtaaatttac tcgcaatgtt gagtaaatta ttcaaaatgt
     7981 acgaacgcac ttatttgaag caggttaagt caagaattga agagccgcga cagttcattc
     8041 aggtaatact tggccctcgc caggtgggta aaaccaccat gataagccaa ttgttgtcgc
     8101 aattgtccat atcatatgta tatgaatctg ccgatgccat aacagcaact aacccggtat
     8161 ggctgatgca ggtatgggag acagccaggc tgaaattgaa agcctccggt gcttcggaat
     8221 acctccttgt tattgatgaa atacaaaaga tagataactg gagtgaagtc atcaggcagc
     8281 agtgggataa agacactcgg gagaaagtaa atatcaaggt tgttttgttg gggtcttccc
     8341 gcctccttat tcagaaaggt ttaacagagt cgttggccgg gcgtttcgag acattttatc
     8401 tcgggcactg gtcgtttgtt gaaatggagg ctgcttttgg ctggtctgtc gaacagtatg
     8461 tttatttcgg aggatatcca ggttctgcat cattggtaaa tgaggaagaa cgttggaaaa
     8521 gctacataaa agatgcactt attgaaacca gtatttcaaa agatattttg atgctgactc
     8581 gtgtggataa gccggcatta ttgaagcgac tatttgaact tggttgctta tactcgggac
     8641 agattttatc ctatacaaaa atcatcggac agttacagga tgcaggcaat accacgacct
     8701 tggcaaatta tctgaaatta ttatctgata gcggtttgtt aggcggattg gggaaatatg
     8761 caggaagtgt aatcaggcaa cggtcgtcca gtccgaagtt ccaggtatat aataatgcat
     8821 tactaacatc gcaggataac gaatcctatt caacagccat tgtaaatcca aaattgtggg
     8881 gcaaactggt tgaatcgtct gttggtgctc atttactgaa tcattcggtt tctgagaggt
     8941 ataatctgca ttattggcga gaaggtaatc atgaaataga ttttatactt gagaagggag
     9001 ataaggtcat aggcctggaa gttaaaagcg gtaccagcac agataacaaa ggaatgggtg
     9061 tttttaatga gaaatttcat cctgataaaa tactgctggt aggcacaggc gggattcctt
     9121 atgaggaatt tctaaagatt aatccaaaag aactttttta acaaaggacc aatacttcag
     9181 gaaaaattgg cattgaatta atctttaaaa cagcctatcg gaaccacata aacaccatct
     9241 tcccggcggt aagcgtattg cccctgacct gtaataacca tcaggaatga agcggatttc
     9301 attcttgtcg tgtctatttt gtcggacaat ctctttagag tatgggcgcc gctctctatc
     9361 aaacggtcac ttcccagttt tatctctatc agaccgaaac agccatttcg cagatgcacc
     9421 acagcatcgc actccaatcc gttcttgtca cgataatggt acacttgccc gtcaagcgca
     9481 tcggcatata cgcgaaggtc gcgtacgacc atcgtttcaa aaagcaaacc gaaggtgttc
     9541 aaatctccga tcaaatcttc gggaccaagg ccaagagcag cagccgcaat ggaggagtct
     9601 acaaaataac gggtgtcaga agtacgtatc gcagtcttgg atcggaggtt aggactccac
     9661 gccggcatgt cttcaataac gaaaattttt ttaagggcta cgatatatga ggcaatggta
     9721 tcttcactca tacttcccat cctgttcttt tccagatcgt catatatcgt gtttacagac
     9781 acttgtgcgc cttgatgacg ggcgtaagag cgcaacagcc ttcgagtaaa ttccgcattc
     9841 cggcttacat cgtccactct tgaaatgtcg ctattagcaa ctgaatccac gtagtttgca
     9901 gcctgtcgca aagcagcctt gggggttaaa ctgagagagc ccggccaacc gcctctgcat
     9961 atcagaaaag ccagttcttt caatgaaata tcacgcgaaa caactggcgt catatttcct
    10021 tgaaaaagag aattcaggct gacgctgcca tttgagtcgc cggattccca caaactcatg
    10081 ggccgcatgg taagccatgc aaaccttccg gtaccggaat gggttaattc tgtcagatcc
    10141 ggaggaacag ccgaacctgt aaaaataagc tgtccgggca aacgcctgcg acttacttca
    10201 aaccgtgcag catcccataa tttaggagcc agttgccatt cgtcaatcag ccttggtgtc
    10261 tctccctgaa aaaggatttc cgggtctgat tccgacagca gcaaattagc cttacgcgtc
    10321 tttggggcat ccatatacag cacacttttt gcctgctgtt ctgccgtagt tgtctttccg
    10381 caccatttcg gaccttggat caaaacgacc cccgatgctt cgagttgttc ttgcagcatc
    10441 ccatctgcta ttcttggctt gtagactacc gatgtttcca tattcttttt tcttgtttaa
    10501 tcctaccgga tttttctatt acaaagataa tataattact ctgttatata gagatatacg
    10561 atggtttatt caccaagttg gctataattt tttcggtaat ttggcttgtt tgcttatcaa
    10621 tgcagagctg ggtagaacac taggttggat attcgtttcg gggaatatgg gtggtactca
    10681 tacagcgcta tcatcagata aagaagatac attttaagag gtttgctctc actggtagaa
    10741 acggttaatt taattcactc ggtttttatg gcttctgaag gattttgtgt ggcggctctg
    10801 taggaatgtc gccagagggt agccatgaaa aatgtgaacg gcctgtcaga tttcgagaga
    10861 gttctctttc agcaggaaat cgaagatgct tatggtggtg ataccttgct cgtcgcgcgt
    10921 gacgggacac tcctcgccaa gtatgattat cttcttaaac gagtcgtaaa tattgagcag
    10981 tgaagcccgt tcctgctact gtttctatgc caagtcagcc ccggctcaca gtcagtttac
    11041 gttcgccgga ggggaggacc tcaagggttg cgtgccaggt gtccggaggc agcaaggggg
    11101 tgatcagctc gggccattca accaaagata aggttccagg agtttcaaaa tattccgtgc
    11161 accccatgtc cagggcttcc tgtatgtttt tgatacgaaa gaaatcaaag tgataaatgg
    11221 ggattccttc agaagaaagg tattcattga ccagggcaaa ggaagggctg ttcacccagt
    11281 cttccgtccc caaaacctga catagggctt tgataaaggt ggtcttcccc gcgcccatct
    11341 gcccgtaaaa agccaccacg ccctccttgc cggtatgttg taaaagcgcc ccggctgcct
    11401 gccgtatgtt ttccagggaa aaaatcagtt ccatgctgca aaaataggta aactattcat
    11461 cttccagtat aaacagatcg ttcacggtct tctcaaaaac ccgggctatc ttcaaagcca
    11521 aaacagtgga aggaacaaat cgttctgttt caatggcatt gatggtttga cggctgactc
    11581 cgcacaaatc cgccagatcc tgttgcgtga ttttcttaat ggcacgctcc actcttatgt
    11641 tattcttcat ttttcagcct ccttttgata atgaacaatc taactctgaa tacaatgaca
    11701 taaataaaga aggcaaaaag tatgttcgag atccagtgca ttacatagta actacgctct
    11761 gtgaagaaat taagccgaaa cccctgggac agtgccgcca gaagggaaaa caaaaccatc
    11821 agggcaaaaa gtacggcagc gactatggta actgaagaaa accgtatttc cctgaccagc
    11881 tcgtcttcct gcttttcctc tgaaaatgcg acaaaaaagg ccgatccgta tgccaacacc
    11941 aacaataggg atgtcacata cctgctgtac tgatccacca ggggaagaaa tgtgttttca
    12001 tggctcgtgt tccattcata tctgagcaaa ataaatagca acaacataac agctaaggaa
    12061 aacatgcccc agccaataac ctggaatttg tgtggaagaa ggattctttt tcttttcatg
    12121 atttacttga tttgtagtat tacttacaaa tgtaaattat attttactat ttgacaagtt
    12181 tgttttacat tttcgggggc ttttcgttaa ttccgtcatt acgttgactg tatttgcatg
    12241 aattccgtca ttatgttgac cgaatttact atatttgctt cagcgcaaaa ttgagtcatt
    12301 tgtttatgga gcaaaaaata cttcttagaa aattgggacc gatcattgaa aaggccatgg
    12361 ttccccaaag ggttgtactt attttcgggg cccgtcgtgt tggaaaaacc attttgctgg
    12421 aaagtattct taaacaggta aaagggaatg taatgctttt aaacggagac gatgcagata
    12481 cactccgttt gcttgaggag cggagcattt caaattacag acgtttatta aacggggttg
    12541 atgtgttggc tattgacgaa gcacaacaaa tccctgagat tggtcataaa ttgaaattga
    12601 tagtcgatac tttacaggac atacgagtca tcgctaccgg atcttcttct tttgatcttt
    12661 tgaataaaag tggagagcct cttgtaggac gagcccgcca atttttgctt acaccctttt
    12721 ctcaattgga actcagccag gaagaaaact tactggaaac aagaagggct ttacctgacc
    12781 gattgatata tggagcttat cccgacgtgg ttaaaatggg gaatgatacg gaccggcaaa
    12841 attacctgga acaaatggca gaagggtacc ttttaaaaga tattctggaa ctggatggaa
    12901 tccgcaattc cgccggattg gtcaatcttt taaaacttat tgctcatcag gtagggaatg
    12961 aagtgtccta cgatgagctg ggtaaacaaa ccggtctgag caggaacaca gttatccggt
    13021 acctggattt gttaagcaaa gtctatatca tctattcttt aagtggctac tcgggcaata
    13081 tgcgaaaaga agtcacaaaa tcccgcaaat ggtattttta cgacaacggg atccgaaatg
    13141 tattgaccgg agatttcagg cccgaagccc ttcgttcaga catgggggcg ttatgggaaa
    13201 actatatggt cagtgagaga gtcaaaaatt cattgaacaa agatgggctt gaaaaatttt
    13261 atttctggag aacgtacgat aaacaagaaa ttgatcttat tgaaaccagc cctgacggct
    13321 ctgtccgggc gtttgaattt aagtggggaa acaagaaacc ccgtgttccc aaaagcttcg
    13381 ccacttctta tccccacgct acattccagg cagtcaaccc ggataattac cacgactttt
    13441 tacaactgta acaaaaagga ggtggaacct cgctttgttg acatggttgt tacttaccac
    13501 ctcccacaaa gcaaccagga acgatgattg gtgctgcgaa gcagcgcctt ttataagcgc
    13561 caaaggcttt tggcgcacca tataagagtg ccccgcacac gttgtcattt tagctgctat
    13621 tcagcgtatt agaagattca tacccctggg ggtatgaatt gcataagtgg ctgattatga
    13681 tgcaaacgaa tcatgtgtgc gaggcaccac ctcccgccag tcagccggag catccccggg
    13741 tcgctgcacc ataaagaacg gagagcctcg gcttaaagaa aaagctctcc attctttatg
    13801 gtgcagcgac cacccggagc atccttacct atactgtatc gcctcggcca ggtggtggat
    13861 ctctatggcc gggctgcctt ccaggtccgc tatggtacgg gccaggcgca ggatgcggtg
    13921 gtgggcgcgg gcagacaggt gcagccgttc cagggcttgc ctgaggaatg tcctgcaggc
    13981 gggaccgagt gcgcatactt c
//
LOCUS       DAOSOF010000042        13994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4384_length_13994_cov_4.931559, whole genome shotgun sequence.
ACCESSION   DAOSOF010000042 DAOSOF010000000
VERSION     DAOSOF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_13994_cov_4.931559"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLM68_03780"
     CDS             complement(<1..230)
                     /locus_tag="PLM68_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOT54518.1"
                     /translation="MINQATIERILEAAPIEAVIGEYVTLKKRGANYIACCPFHNEKT
                     PSFSVSPSRGIFKCFGCGKAGNVVRFIMEHEQL"
     gene            complement(230..1219)
                     /gene="trpS"
                     /locus_tag="PLM68_03785"
     CDS             complement(230..1219)
                     /gene="trpS"
                     /locus_tag="PLM68_03785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT54519.1"
                     /translation="MKTVLSGIRSTGHLHLGNYFGALRNFVQLQHQAKCYFFVADLHS
                     LTTHPKPDDYLSGVKTILSEYLASGLDPEKSTLYVQSSVPQVCELYVLLNMLTYKGEL
                     ERTASFKDKVRLNPDNVNAGLLTYPVLMAADILIHRANWVPVGKDQEQHLEITRMLAR
                     RFNKLYGAEVFPEPEAYNFGGHLVRIPGLDGSGKMGKSENNGIYLVDDQKTITKKVMR
                     AVTDTGPVNPNDPMSPGVANLFTLLGVVSEPEVVAYFREQYDKCTIRYGDLKKQLAAD
                     ICKHTLPIKERIEEIYNNGPYLKYVIEKGTEEARTSARATMELVRDAVGFKTF"
     gene            1287..2639
                     /locus_tag="PLM68_03790"
     CDS             1287..2639
                     /locus_tag="PLM68_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT54520.1"
                     /translation="MITISKGSLKGKVTLPPSKSMSIRVLAAIFLSSRDTLLKGAWPK
                     DGTQVCEDVQAAYRVVRSVGSQFAKEDIWPNMAPPGRDHSRRQLDCGESALALRMFAP
                     IVALSSYIYCLRGRTTLITRPVDFVIQGLRELGCWCESLNTLPTLSIMENLKETGRWE
                     PRDSSLSLSRPILLVQGPLRGGKYQVNAGGSSQFLSGLLMALPLAEKDSILEVEHLAS
                     QPYIDLTLNVMESFGVRATHDNYKVFHIPGRQSYRAHKIDIEGDWSGGANFIVGAAMF
                     GDLRISPLWKDSLQADKAILEAVGKAGGSWAWGSANQLKVYKNDNLQGFSFDAQQCPD
                     LFPPLVALAVHCKGTTTIHGIDRLFHKESNRALALLEEYSKVGADIRLEGNNMVIKGG
                     VLPGGARISARGDNRIAMSLAIAALGAEKPIEIEGKHTVAKSFPDFWEELNNIRIPLS
                     "
     gene            2636..3577
                     /gene="aroC"
                     /locus_tag="PLM68_03795"
     CDS             2636..3577
                     /gene="aroC"
                     /locus_tag="PLM68_03795"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT54521.1"
                     /translation="MNVFGRNFRVAVYGESHGPEVGVLLDGVPPGILLSEIDFQEDIA
                     RRKSGAPGTTRRQEADEPLIRSGMYRGYTSGSPLCISFLNRDARSQDYLPSEDSDPVQ
                     KLPHRPGTADYTGDVKFRGFQDPRGGGHFSGRLTLPLVAAGVVAKRILAGRFPDMTIT
                     AFLTQVGGIPVIDKRALPGEVNTLLEQAVEQADSLGAVISCEVTNIPAGLGNPFFDSV
                     ESLLSHGVFSIPGVKGITFGLGFDESLLMGSQNPHIGGIAGGITNGDMVSFQVAVKPT
                     PTVGGKGRHDACFALRVPVVVEAVTAMVIADLMMTSA"
     gene            complement(3585..4622)
                     /gene="recA"
                     /locus_tag="PLM68_03800"
     CDS             complement(3585..4622)
                     /gene="recA"
                     /locus_tag="PLM68_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT54522.1"
                     /translation="MAKVSAEEVKAEGVTASQEKMKALQATLDKIEKDFGKGTIMKLG
                     DQPKWDASVIPSGSIGLDHALGIGGYPRGRVVEIYGPEASGKTTLAIHAIAEAQKLGG
                     IAAFIDAEHAFDRNYAQQLGVNVDTLLISQPDNGEQALEVADHLIRSGAIDIVVIDSV
                     AALTPKAEIEGEMGDSKMGLQARLMSQALRKLTANISRTNTCCVFINQLREKIGIMFG
                     NPETTTGGNALKFYASVRVDVRRTTQLKDGEDPTGNHVRVKVVKNKMAPPFKKAEFDI
                     IFGEGISLIGEIIDLGVEFEIIKKSGSWFSYGETKIGQGRDAVKQILKDNPELKEEIE
                     AKVRAVMQQQK"
     gene            4690..5715
                     /gene="rlmN"
                     /locus_tag="PLM68_03805"
     CDS             4690..5715
                     /gene="rlmN"
                     /locus_tag="PLM68_03805"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT54523.1"
                     /translation="MKEWLLGKNPEQLRQIAASYGLPAFAAGQIANWIYARHVREIAD
                     MTNLSRAAREKLAETYQVGGFEPLQVQVSTDGTKKYLFPTLCVSRTGVEAVMIPEGDR
                     ATLCLSSQVGCKLGCSFCMTGRMGFAGNLTAGEIVGQFLGTGETDRLTNIVYMGMGEP
                     LDNWDQVLRSLEIFTADWGFAWSPRRITVSTSGILPVLDDFMAKTQAHLAISLHSPYD
                     EQRAQIMPVQKAFPISSVVQKLRQYDFSGQRRLSFEYILFDGWNDTLRHAAALLQLLR
                     GLECRVNLIRFHAIEGFPMRPSAEPAVNAFMTRLNRAGLVATVRASRGEDILAACGQL
                     SVKKNEK"
     gene            5712..6188
                     /locus_tag="PLM68_03810"
     CDS             5712..6188
                     /locus_tag="PLM68_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT54524.1"
                     /translation="MMEQETYNKHLARMQALCSRSEKCRADIRLKLEQAGVSPAETEA
                     ILNALEKERFLDQERYATAYARDKSNLQGWGPRKIYNMLRIKEIPEEVIEKALSGLGE
                     EQIRQRLTEALKKHRSFREKDPRKRYAALVRFGLGRGYSYAQIVDCLGKINESKHE"
     gene            6534..7079
                     /locus_tag="PLM68_03815"
     CDS             6534..7079
                     /locus_tag="PLM68_03815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOT54525.1"
                     /translation="MVTLQAGDILFTLTRSARKSVGLTVKKDRTVHVRAPFWLSSSEV
                     IRMVSGKASWIRSRQQALEKEQVLPPSGPELNRIKKQAELKFHTIASPWVEHFREQYN
                     VSPKRWTIRNMNTRWGSCSKSTGRITLNLKLFYKPDACIEYIIVHELCHLIHPDHGKN
                     FYALLQRELPDWKVRRKQLKA"
     gene            7092..8178
                     /gene="prfB"
                     /locus_tag="PLM68_03820"
     CDS             join(7092..7157,7159..8178)
                     /gene="prfB"
                     /locus_tag="PLM68_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143822485.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT54526.1"
                     /translation="MIYNEDNYKNLLQRGQRLRRFLDVEAREKELKTLTETSQQPGFW
                     EKPKEAKALLRRQSLLKASLEAYYRIKEGLDDLQVLDQLEAPQEELDAQITKVLEWLR
                     KEELKSMLGNEGDTMGALLRINSGAGGTESNDWSAMLMRMYVRWGERNGYKVRVHNIL
                     DGEEAGIKSCTLEFEGLYAYGYLKAENGVHRLVRISPFNAQGKRQTTFSSVFVYPAVD
                     DTIEIEINPSDLEWDTYRSSGAGGQNVNKVETGVRVRHIPTGIVVENTETRSQFDNRQ
                     NALRILKSHLYEIELEKRREKEAQLEGQKKKIEWGSQIRSYTLHPYKMVKDHRTGYEM
                     GDTQSVLDGDLNGFIQAYLLGGSANGS"
     gene            8168..11311
                     /locus_tag="PLM68_03825"
     CDS             8168..11311
                     /locus_tag="PLM68_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT54527.1"
                     /translation="MALKVYRASAGTGKTYRLTLMYLSLLLGNAARFDPRAFYGILAV
                     TFTNKATDQMKTRILDTLESLAAGKVSALGSDLCAETGLPFEELAARAATVHKTLLHN
                     YSWFSVSTLDAFFQRVLHSFVMEAGLGTGYSVDTDTEYLLDKTARKMITRITQDDALR
                     LWFTDLINEKIARSDRWDALRLLRQVGFQATRESFRTMGEPFAKKISDREFLRDFMGK
                     CQLIIDEFEQRMAAWGKQALELISARGFSTTDFPNKVSSFARYFEKLTDPYPAVDTFI
                     PGKRVLEAVDGTEAVWHNKNSPTGLETMQRVLMPVLRSIVDHYHSHYQQYATAVAIRE
                     QLPQMGLLADVITTMRQVQEEDNTLDIGDSTYLLSQLAGDGNTPFIYERIGAGYDSFL
                     LDEFQDTSYLQWKNMHPLLVNGLSQGSDSLIVGDVKQSIYRWRNSDWKILGEEIAKDP
                     QLIRQGVDFYTLNTNRRSGREIIGFVNTLIDNVIETITEEVRKKTSACGNLETGDREY
                     FEQMLHVAYRDHTLHTPGDRTEDPHGYVQVNTFSAEAEKDTTEKALSSLQDLLGSLLS
                     RGYSPSDILILVRSRENARTITRHFLRNTNTGKGEGHPCPRVVSEDSLFLASSPAVNL
                     VIALLKQAHYRHDTTNARVASSLLSGFGKPEVSDDREFLDRLRYLPLADAFEAIMQRM
                     DWTGRLEALPYLQELHDMLLLFAGREGGGVYAFLNWWYERGKEKMLSTGLPVQAVRVL
                     TIHKAKGLEAKVVIVPFCNWPLDSRSGSQVIWAGTSREPFNQPEQLPVLYNDRLLNTY
                     FARDYFMEHTQRNLDSLNLLYVAITRAREELYIFLPENEKSSSTDISFHIRKALFQTM
                     EDGDLAWTCGEQQTPARQSTADDSHGFRPRAYPSGDFRNKLKLVYSEDDFEYAAGDSM
                     RQWGIVLHKALARLEKQEDLAGVLEELVMEGELSGNKETVTGFARIIEKTLSDPQVSL
                     WFDGSWTARNESSILGPSGKQRPDRIMEKDGRVVVLDYKFGKPDPAHHRQMNRYVAAL
                     KRMGYIQVEGRLLYFTP"
     gene            complement(11308..12939)
                     /locus_tag="PLM68_03830"
     CDS             complement(11308..12939)
                     /locus_tag="PLM68_03830"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOT54528.1"
                     /translation="MKSNISRRSFLKKSILGGTGLVATAGGLSASSIFRASQPFDTII
                     QNGLICTGDGTAPYRGDVGIKDGRIAALGRLGNYADHLVDVDGMAISPGFVDLHSHTD
                     TNLFQCPRGDSRIFQGITTEAGGNCGDSPFPVKPYENASSFLEALRNQKTGINYCTFT
                     GQGSIRSAIIGEWDRAATAEQQVAMKDLLEAQLEQGSIGLSCGLEYAPGAYASNEELV
                     GLLKVVAKHNGLFSIHMRNEDDRVEEAVSEAIAMARESGVRLQISHLKAQNFNNWHKG
                     PSLIRLIENARRDGIDIAFDRYPYIAFSTGLTSFIPLDGRQGTMEDVLSRLRDKETDL
                     AFGEYARSRFARFGGTRNVIISACSLDHNKETYMGRNLEECARISGQDAWDFVRDLLI
                     EERLQCSIVVFAMTEDNVRLFLAQPLGMPASDGSVYSPRGPLSETRPHPRSYGTFPRF
                     LGKYCREGKLMDFSAAVRKITSLPASRLGLKERGLLVPGYHADVVVFDPATVIDRASF
                     EDPHRFPEGIPHVWVNGVHTIRNGSHTGVLAGMVL"
     gene            complement(12917..13921)
                     /locus_tag="PLM68_03835"
     CDS             complement(12917..13921)
                     /locus_tag="PLM68_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT54529.1"
                     /translation="MRRILFPSMATLICLLVIAGCTPEQEYQTIKGVVMGYSYQIVYQ
                     APDYAPWSFKSDIKKAARKCAREIDRSISLYNPSSIINSVNTNKSMETDSIFAAVLDK
                     TKATAELTGASMNTTTGPLRELWGNNPGNTKNVTEDQIARVLEYSGIWKVSVQNGLFV
                     KEDPRIQLDLSRTGIGFAADYLGSMLDSNNISNYMIEVGKVVLCKGLNPSGTPWRYLI
                     QQTYGNVIYLTDKSMSICGDLSDFKPGLGKTFNGFFNTRTGYPVEDPILSILVIANNG
                     LDAAASSVAFYSLGLEKSIQYLESHPDIQAIMYYCNNDSISTYITPNITQRYEIQYFP
                     "
BASE COUNT         3491 a         3447 c         3695 g         3361 t
ORIGIN      
        1 agctgctcgt gttccatgat aaaccggacc acattgcctg ccttcccgca accaaagcat
       61 ttgaagatac ctctggatgg agagactgaa aaagaagggg ttttttcatt atggaaaggg
      121 caacaggcta tgtaattggc gcctcgtttt ttcagggtga cgtattcgcc aatcactgcc
      181 tctatgggag cagcttccag gatgcgttct atggtggcct gatttatcat cagaaagttt
      241 tgaatccgac cgcatcacgt accagttcca tggtggcgcg tgcagaagta cgggcctcct
      301 ccgttccttt ttcaataacg tacttcagat agggcccgtt gttatatatt tcctctatcc
      361 tttcctttat gggcaatgta tgtttgcaga tatcggctgc cagctgtttc ttcaggtcac
      421 catagcgaat ggtgcatttg tcgtattgtt cccggaaata tgcaaccact tcaggttcgg
      481 ataccacgcc cagcagtgtg aagaggttgg caacccccgg gctcatggga tcattcggat
      541 ttacaggccc agtgtcggtt acggcacgca tcactttttt tgttatagtc ttctggtcat
      601 ccaccaggta aatgccgttg ttttcgctct tgcccatctt cccgctgcca tccaggccgg
      661 ggatcctgac caggtggcct ccaaaattgt aagcttcggg ttcagggaaa acctctgccc
      721 cgtacaactt gttgaatctt cttgccagca tccgtgtgat ttccaggtgt tgttcctggt
      781 ctttacccac aggaacccag ttggcacggt gaatcaggat atcagcggcc atcaggaccg
      841 gataggtcag cagtccggcg ttgacgttgt ccgggttcag gcgcaccttg tccttgaaag
      901 aggcggtgcg ttccagttct cccttgtatg tgagcatatt gagcagcaca tacaattcac
      961 aaacctgggg aacactgctt tgcacataca gggtggactt ttccggatcc agtccggatg
     1021 caaggtattc agaaaggatg gtcttcacgc cggagagata atcatccggt ttgggatgcg
     1081 ttgtcaggga atggagatcg gcaacaaaga aataacattt ggcctgatgc tgcaattgga
     1141 caaaattccg gagagccccg aagtaattgc ccagatgcag gtggcccgtg gaacgtatgc
     1201 cgctcagaac agttttcata ggaaggagat tgaaaattca accgacaaag ttagtctttt
     1261 ttgggtaaat gttatctttg tataatatga taaccatttc aaaaggttcg cttaagggta
     1321 aagtaacact gccgccatct aaaagcatga gtattagggt attggcggca atattcctgt
     1381 ccagtcgcga taccttgctc aaaggagcct ggcctaagga cgggacccag gtctgtgaag
     1441 atgtacaggc tgcgtaccgc gtggtccggt ctgtgggttc acaattcgcg aaggaagata
     1501 tctggcccaa tatggctccg ccgggaaggg atcattcaag acgccagctc gactgcgggg
     1561 aatctgccct tgccctgagg atgtttgctc ccatcgtggc cctttcgtcc tatatctatt
     1621 gcctgagggg tcgaaccacc ctcatcacaa gacctgtgga tttcgtcatc cagggacttc
     1681 gagagctcgg atgctggtgt gaaagcctca acacccttcc cactttatcg atcatggaaa
     1741 acctgaaaga aacaggcagg tgggaaccac gtgattcttc actgagcctg tcgcgtccca
     1801 tattgctggt gcaaggtccg ctcagggggg gaaaatacca ggtcaacgcc gggggcagtt
     1861 cgcagttttt gagcgggctt ctgatggctt tgcccctggc cgagaaggac tccatactgg
     1921 aagtggagca tctggcaagc cagccatata ttgacctgac attgaatgtg atggagtctt
     1981 ttggcgtacg tgcaacacat gataattata aagtgttcca tattcccggg aggcaatcct
     2041 accgggcaca caagattgat attgagggcg attggagcgg aggggcgaat tttattgtgg
     2101 gagcggccat gttcggagac cttcggattt ctcccttgtg gaaggacagc ctgcaggccg
     2161 acaaagcgat actggaagca gtcggtaagg caggcggttc gtgggcctgg ggatcggcca
     2221 atcagttgaa ggtatacaag aatgataatt tgcaaggctt cagttttgat gcgcagcaat
     2281 gtcccgacct gttcccgcct ttggtcgcgc tggcagttca ctgcaaaggg accaccacca
     2341 ttcacggtat agacaggctc ttccacaagg aatcaaacag ggccctggcc ttgctggaag
     2401 aatattcaaa agtgggtgcg gacatacgtt tggaagggaa caacatggtg ataaagggag
     2461 gcgtccttcc gggaggggcg cgcatttccg ccaggggaga caaccgcata gccatgtcgc
     2521 tggccattgc agccctgggc gccgaaaaac ccattgaaat tgaaggcaag catactgtgg
     2581 caaaatcttt tcctgatttt tgggaagaat tgaataatat aagaataccc ttatcatgaa
     2641 tgtcttcgga agaaatttca gggttgccgt atacggtgaa tcacacgggc cggaagtagg
     2701 agtactgttg gacggcgtgc ctccgggcat cctgctatcg gaaattgact ttcaggagga
     2761 tattgcgcgc cggaaaagcg gagctccggg caccaccagg cgccaggagg cagatgaacc
     2821 tctcatccgc agcggtatgt acaggggata cacctcggga agccccttgt gcatatcatt
     2881 tttgaaccgg gatgccagat cacaggatta cctgccctca gaggacagtg atcctgtaca
     2941 gaaacttcct cacaggccgg gaacagctga ttacacggga gatgttaaat tccgtggttt
     3001 ccaggacccg cggggaggag ggcatttttc aggcaggctg accctgccac tggttgcggc
     3061 aggtgtggtg gccaagagga tcctggccgg caggttcccg gatatgacca ttacagcatt
     3121 cctgacgcag gtgggaggca tacctgttat tgacaaaaga gcacttcccg gggaagtgaa
     3181 caccctgctt gaacaggcag tggaacaggc tgattccctg ggggccgtca tttcctgtga
     3241 agtgaccaat atccccgccg gccttgggaa tcctttcttt gattcggtag agtcgttatt
     3301 gtcccatggt gttttttcca ttcccggagt caaggggatc acttttggac tgggattcga
     3361 cgaatcgttg ctcatgggat cacaaaaccc gcatataggg ggaatagccg gaggtattac
     3421 aaacggcgat atggtttcat tccaggtggc cgtcaagcct acccccacgg tgggaggcaa
     3481 aggcaggcat gatgcctgct ttgcccttcg tgttcctgtc gtggtggaag ctgtaactgc
     3541 aatggtcatt gcagatctga tgatgaccag tgcttaacgc attgtcattt ctgttgctgc
     3601 atgacagcac ggactttggc ctcgatctct tccttcagtt ccggattgtc tttgagtatc
     3661 tgtttgacgg catcgcgtcc ctgtccgatc tttgtttccc cgtagctgaa ccaggacccg
     3721 ctttttttga tgatctcgaa ttcaacgccc aggtctatga tctctccaat gagcgaaata
     3781 ccttctccga agatgatgtc aaactcggct tttttaaaag gaggggccat cttgttcttg
     3841 accactttta cgcgaacgtg gttgcctgtt ggatcctccc catccttgag ctgagtagta
     3901 cggcgaacat ccacgcgcac cgaagcgtaa aacttcaggg cgttcccccc ggtggttgtt
     3961 tcggggttgc cgaacatgat cccgattttt tccctgagct ggttgataaa gacacagcag
     4021 gtattggtcc ggctgatatt ggctgtcagt ttcctgagtg cctgtgacat caggcgtgcc
     4081 tggagcccca ttttcgaatc ccccatttct ccctctatct cggctttggg agtcagggcg
     4141 gctaccgagt caatgaccac aatgtcaatg gctccggagc ggatcaggtg gtctgccact
     4201 tccagggcct gttccccgtt gtcgggctgg gatatgagca gggtatccac attgacaccc
     4261 agctgttgcg cataattgcg gtcaaaggcg tgttcagcat cgataaatgc tgcgatgcca
     4321 cccagtttct gtgcttctgc aatggcatga atggctaaag tggtcttccc gctagcctcg
     4381 gggccatata tttccactac acggcccctg ggataaccgc caatgcccag ggcatggtcc
     4441 aggccaatgg aacctgacgg tatgacagat gcgtcccatt tgggctggtc tcccagtttc
     4501 atgatggtcc ccttcccgaa atccttctca atcttgtcca gggttgcctg gagcgctttc
     4561 attttttcct gactggcggt tactccttcc gcttttactt cttctgcact aacttttgcc
     4621 ataatattct gattaatgat tatacgacaa atatacaaat tcactgacaa aaacccgtat
     4681 ctttgaagta tgaaagaatg gttgctgggg aaaaatccgg agcagttgcg gcaaattgct
     4741 gcttcttacg gcctgcctgc atttgctgcc ggtcagattg ccaactggat atacgccagg
     4801 cacgtcaggg agatcgcaga catgaccaac ctgagcaggg cggcacggga aaaactggcc
     4861 gaaacatacc aggtgggcgg ctttgaaccg ttgcaggtgc aggtgtcaac agatggcact
     4921 aaaaaatatc ttttccccac cttatgcgtt tccaggaccg gggtagaagc ggtaatgatc
     4981 ccggaagggg accgggccac gctgtgtctt tcttcccagg ttggctgcaa actgggttgt
     5041 tctttctgca tgacagggcg catgggtttc gcaggcaacc tgactgccgg agaaatagtg
     5101 ggacagttcc tgggaaccgg agaaacagac cggctgacca atattgtata catgggcatg
     5161 ggagaacccc tggacaattg ggaccaggtg ctcaggtccc tggaaatatt caccgctgac
     5221 tggggttttg cctggagccc caggcgcatc accgtttcca cttccggcat attgcctgtg
     5281 ctggatgact ttatggcaaa gacacaggca caccttgcca tcagccttca cagtccctat
     5341 gatgagcagc gcgcacagat catgcctgtc cagaaagcct ttcccataag cagtgtcgta
     5401 cagaaattac gtcaatatga tttttcggga cagcggcggc tcagctttga atacatcctg
     5461 ttcgacggct ggaacgatac gctaaggcac gcggccgccc tgttgcaact cctgcgcggc
     5521 ctggaatgcc gggtcaacct gatacgtttc cacgccatag aaggtttccc catgcggccg
     5581 tctgccgagc cggcagtgaa cgccttcatg acccgtctga acagggccgg cctggtggca
     5641 acggtgcgtg cttccagggg cgaggacatc ctggctgcct gtggccagtt gagcgtaaaa
     5701 aaaaacgaaa aatgatggaa caggaaacat acaataagca cctggcccgt atgcaggctt
     5761 tatgcagccg cagtgaaaaa tgcagggcgg atatccgcct gaaactggag caggcaggcg
     5821 tatcccctgc ggaaacagaa gccatcctga atgcactgga aaaggaaagg ttcctggacc
     5881 aggaacgtta tgcaacagcc tacgcaaggg ataagtccaa tttgcaggga tggggccccc
     5941 ggaagatcta caatatgtta aggattaaag aaataccaga agaggtaata gaaaaggcat
     6001 tgtccggatt gggggaagag cagatccggc aaaggttgac agaagccctt aaaaagcacc
     6061 ggagtttcag ggaaaaagat ccccgcaagc gttacgccgc cctggtgcgt tttggtctgg
     6121 gcaggggtta cagttatgct cagattgttg attgtctggg aaaaattaat gaatcaaagc
     6181 atgaataatt ttttggttaa ttaaaaaact tgttatattt gtgtttcata taagtagcca
     6241 tttactttat taactaaaat actaacgtca tgacaatttt agatttcaat ttgcaaccga
     6301 tttacaatgc tttggcaaat ctctggacca ctctttttgg taatgcagga gaatgggctg
     6361 acaatttttg gacggtcatt aaccaaatct gggatatttt ccaggaaatt ttcaccttcg
     6421 taattcacac tcctgttgcg taatccacac ttaatctatc cactctggat tacaaacgct
     6481 gatcagtaaa caaatctaaa gctgtctcgt acgaaggcag ctttttttta tagatggtga
     6541 cgttacaggc tggtgacatt cttttcaccc tgaccaggag tgcccgcaaa tctgtgggac
     6601 tgaccgtcaa gaaggacagg acagtacacg taagggctcc tttctggctc tcttcctcgg
     6661 aagtgatccg catggtgtcc ggcaaggcat cgtggatacg ctccaggcag caggcacttg
     6721 aaaaggaaca ggtactgccc ccttctggac cggagttgaa ccggataaaa aaacaggcag
     6781 aacttaagtt tcacaccatt gcttctccct gggtggagca ttttcgtgaa caatacaatg
     6841 tctccccaaa acggtggacc atccggaaca tgaatacccg ttggggcagt tgttcaaaat
     6901 ctacggggag gatcacgctc aacctgaaac tgttttataa gcccgatgca tgcattgaat
     6961 atattatcgt gcacgaatta tgtcacctga ttcaccctga ccatggaaaa aacttttatg
     7021 ccttgctgca aagggagctt ccggactgga aagtaagaag aaaacaatta aaagcttaac
     7081 tttgtgccat tatgatatac aacgaggaca attataaaaa cctgctgcag cgcgggcagc
     7141 ggttgaggag gtttctttga cgtagaggcc agggaaaaag agttgaagac attgacagaa
     7201 acctcgcaac agcctggttt ctgggaaaag cccaaagagg ccaaagcgct cctgaggaga
     7261 caatctttgc tgaaggcttc gctggaagca tattacagga tcaaagaagg tctggacgac
     7321 ctgcaggttc ttgaccagct ggaagccccc caggaagaac tcgacgccca gataaccaaa
     7381 gtgctggaat ggttaaggaa agaagagctg aaaagcatgc tcggcaacga gggcgatacc
     7441 atgggcgcct tgctcaggat caattccggg gctgggggaa ccgagagcaa tgactggtct
     7501 gccatgctca tgcggatgta tgtccgctgg ggagaaagga atggctataa agtcagggtg
     7561 cacaacatcc tggatggaga agaggccggt atcaagtcct gcaccctgga atttgaaggc
     7621 ctctatgcct acggctatct gaaagccgag aatggggtgc accgcctggt gcggatatca
     7681 ccattcaatg cccaggggaa aagacagacc actttctcct ctgtatttgt gtatcctgcc
     7741 gttgacgaca ccattgaaat tgagatcaac ccctccgatc tggagtggga cacctaccgt
     7801 tccagcggag cggggggaca aaatgtgaat aaggtggaaa ccggggtgcg tgtccgccac
     7861 attccaacgg ggatcgtagt cgagaatacg gaaacaagaa gccagttcga caaccggcag
     7921 aatgccctgc gtatccttaa atcgcatttg tacgagatag agctcgagaa acgtcgcgag
     7981 aaggaagccc agctggaagg ccaaaagaaa aaaatcgaat ggggttcgca gatcagatcc
     8041 tatacactgc atccctataa aatggtcaag gaccaccgta ccggttatga aatgggcgat
     8101 acacagtctg tactggacgg agacctgaac gggttcattc aggcatacct cctgggagga
     8161 agcgcaaatg gctcttaaag tatacagggc atcggcaggt acaggcaaaa cgtatcgcct
     8221 gaccctgatg tatctttccc tgttgctggg caacgctgcc cgtttcgatc cgcgtgcctt
     8281 ttacggaata ctggccgtga cttttaccaa taaggccaca gaccaaatga aaaccaggat
     8341 cctggatacc ctggaatcac tggctgcggg aaaagtctcc gccctgggaa gcgatctttg
     8401 cgcggaaaca ggccttccct ttgaagaact tgcagcgcgc gctgccacag tccacaagac
     8461 gctcctgcac aattactcct ggttttctgt atccaccctg gatgcttttt tccagcgggt
     8521 tttgcactcc tttgtcatgg aagccggtct gggcacgggt tattccgtgg atacggacac
     8581 ggagtacctc ctggataaga cagcccgcaa aatgatcacc cgtatcacgc aggatgatgc
     8641 actccgcttg tggtttacag acctgatcaa tgaaaagatt gccaggtcgg accggtggga
     8701 tgcgctccgc ctgctcagac aggtgggttt ccaggctaca cgcgaatcgt tcaggaccat
     8761 gggggagcct tttgccaaaa aaatctcgga ccgggaattc ctgcgggatt tcatgggtaa
     8821 atgccagctc ataattgatg aatttgagca acggatggcc gcctggggaa aacaggcact
     8881 ggagctgata tctgccaggg gattttccac gaccgatttt cccaacaaag tatcttcctt
     8941 tgcccggtat tttgaaaagt tgacggatcc ctatcccgcc gtggacacct tcatacccgg
     9001 aaaacgtgta ctggaggcag tggatggaac tgaagccgtc tggcataaca agaattcacc
     9061 cacgggcctg gaaacaatgc aacgggtact gatgcccgta ctgaggagca tcgtggatca
     9121 ttaccatagc cattaccagc aatatgcaac cgctgtagcc atcagggaac aattgccgca
     9181 aatgggactg ctggcagatg tcatcaccac catgcgccag gtccaggaag aggacaacac
     9241 gcttgacata ggcgattcca cgtacctgct cagccagctg gcaggtgacg ggaacacccc
     9301 tttcatttat gagcggatag gggcagggta tgattctttt ctcttggatg aattccagga
     9361 cacatcgtat ctgcagtgga agaatatgca cccgctgctt gtcaacgggt tgtcacaggg
     9421 atcagattca ctcattgttg gggatgtcaa acagtccata taccgctgga gaaacagcga
     9481 ctggaagata ctgggagagg aaattgcaaa agacccgcag cttatccggc agggggtgga
     9541 tttttatacc ctcaacacaa accgcagaag tggccgggaa atcattggtt ttgttaatac
     9601 tctgatagat aatgttatag aaacaatcac agaagaggtc aggaaaaaaa ccagtgcctg
     9661 cggcaacctg gagactgggg atcgcgagta tttcgaacaa atgttgcatg tcgcgtacag
     9721 ggatcacacc ttgcatacac ccggggacag gacggaagac ccgcacggat atgtgcaggt
     9781 caacaccttc tcggctgaag cggaaaaaga cactacggaa aaggctctct cttcattgca
     9841 ggatttgctg ggatcattgc tctccagggg gtacagtcct tccgatatat tgatcctggt
     9901 caggtccagg gagaatgcac gtaccattac gcggcatttc ttaaggaaca ccaataccgg
     9961 gaaaggggaa gggcatccct gtccccgtgt ggtgtcagaa gactcgctct ttctggcgtc
    10021 ttctcccgct gtcaaccttg tgatcgcttt actcaaacag gcacattaca ggcatgatac
    10081 gaccaatgcc agggttgctt cctctttgtt gtccggcttt ggaaaacctg aagtgtccga
    10141 tgacagggag ttcctggatc gcttgcgtta cctgcccctg gcagatgcat ttgaagccat
    10201 catgcaaagg atggattgga ccggccgcct tgaggccttg ccttaccttc aggaattgca
    10261 cgacatgttg ctcttgtttg ccggcaggga aggaggcggg gtatacgcct tcctgaactg
    10321 gtggtatgag aggggaaaag aaaaaatgct ctccacaggc cttcccgtcc aggctgtcag
    10381 ggtgctgacc attcataaag ccaaaggact ggaggccaag gtcgtcatag tacctttttg
    10441 taactggccg cttgattccc ggtcgggcag ccaagtgatc tgggccggca cttcccgtga
    10501 gccattcaac cagccggaac aattacctgt tctatacaac gacaggctcc ttaatacgta
    10561 ttttgccagg gattacttta tggaacatac gcagagaaac ctggattccc tgaatttact
    10621 ctatgtggcc ataaccaggg ccagggagga attgtatatc ttcctgcctg aaaatgaaaa
    10681 atcatcaagc actgacatat cctttcatat aagaaaagcc ctttttcaga caatggagga
    10741 cggggacctt gcatggacct gcggcgagca acagactccg gcaaggcaga gcacggcaga
    10801 tgacagtcat ggattcaggc cccgggctta tccctcgggc gatttcagga acaaattgaa
    10861 actggtttac agtgaagatg attttgaata tgctgccggg gactcaatgc gacaatgggg
    10921 tattgtattg cacaaagccc ttgcgcgcct ggaaaagcag gaagatctgg caggcgtgct
    10981 ggaagaattg gtcatggaag gagaactctc gggcaacaag gagacagtta cgggtttcgc
    11041 ccggatcatt gagaaaacac tctcggaccc acaagtttca ttatggtttg atggttcctg
    11101 gactgccagg aacgagtcct ccatactggg gccgtcggga aaacagcgtc cagaccggat
    11161 aatggaaaag gacggcaggg tagtggtact ggattacaaa ttcgggaagc ccgaccctgc
    11221 ccatcacagg caaatgaacc gttatgttgc cgccctgaaa aggatgggct atatacaggt
    11281 ggaaggcagg ctgctgtatt tcacacccta aaggaccatc ccggccagaa cgccggtatg
    11341 cgagccgttc cggatggtgt gtaccccgtt gacccataca tgcgggatgc cctcagggaa
    11401 acggtgcgga tcttcaaaag aggccctgtc aatgaccgtt gccgggtcaa aaaccaccac
    11461 atcggcgtga tatcccggga caagaagtcc cctttccttc aagccgagcc tggatgcagg
    11521 cagtgaggtg atttttctca cagctgccga aaaatccatg agctttcctt ccctgcaata
    11581 tttaccaagg aaacggggaa aagtgccata ggaacgggga tgcggcctgg tttcggacaa
    11641 ggggcctcgg ggagaataaa cacttccgtc cgaagcaggc atacccaggg gttgtgcaag
    11701 gaaaaggcgg acattgtctt ctgtcatggc aaaaacaaca atgctgcatt gcaggcgttc
    11761 ttcaatcaac aggtccctaa caaaatccca cgcgtcctgt ccggaaatgc gggcacactc
    11821 ttccaggttc ctgcccatgt atgtttcttt gttatggtcc aatgaacagg cagatatgat
    11881 aacattgcgg gtccctccaa aacgtgcgaa acgggaacgt gcatattccc cgaatgcaag
    11941 gtctgtctct ttatccctga gccgggacaa cacatcctcc atggtcccct gtctgccgtc
    12001 caggggtatg aaactggtca gtcccgtgga aaaggctata taaggatagc ggtcaaaggc
    12061 aatatctata ccgtcacgtc tggcattttc tatcagccgg atcagggaag ggcctttgtg
    12121 ccagttatta aaattctggg ctttcagatg tgagatctgg agcctcacgc ctgactcccg
    12181 cgccatggca atggcttccg atacagcttc ttccacccgg tcgtcctcat tgcgcatgtg
    12241 tatggaaaat aagccattgt gtttggcaac cactttcaat aatccaacaa gttcctcatt
    12301 ggaggcatag gcgcccgggg catactccaa gccgcacgat aatccaatgc tgccctgttc
    12361 cagctgggct tccagcagat ccttcatggc aacctgctgc tcagcggtgg cagctctgtc
    12421 ccattctcca atgatcgcag agcggatgga accctggccc gtgaaggtgc agtaattgat
    12481 ccctgttttt tgattcctca aggcttccag aaaagatgat gcgttttcat acggcttcac
    12541 cggaaagggt gaatccccgc agttgccgcc tgcttcggtg gtgatgccct gaaagatacg
    12601 gctgtctccc cggggacatt gaaaaagatt tgtatctgta tggctgtgca ggtccacaaa
    12661 tccggggctg atggccattc cgtcaacatc tacaagatga tcggcataat tgcccagccg
    12721 gcccaatgca gctatgcgtc catccttgat gcccacgtct ccgcgatatg gagctgttcc
    12781 gtccccggta cagataagcc cgttttgaat gatggtatca aagggctggg aggcgcgaaa
    12841 gatagaagaa gccgagagcc ctcctgcggt tgctacaagc ccggtaccgc ccaggatgct
    12901 ttttttgaga aaagacctac gggaaatatt ggatttcata acgttgagtt atgttagggg
    12961 ttatgtacgt cgatatagaa tcattgttgc aataatacat gatagcctgg atatcgggat
    13021 gagactcaag gtactgaatg cttttctcca gccccaggga atagaaggca acactactgg
    13081 cagctgcatc caaaccattg ttggcaatca ccaaaatact caggatggga tcttctaccg
    13141 ggtagcctgt tcttgtattg aaaaagccat tgaatgtttt cccaaggccg ggtttgaaat
    13201 cggacaagtc gccacatata gacattgact tgtcggtcag atagatcacg ttcccgtaag
    13261 tctgctgaat cagataacgc cagggtgtcc cggaaggatt cagacctttg cacaaaacaa
    13321 cttttcccac ttctatcata taattggata tgttgttcga atccagcatc gagcccaggt
    13381 aatcggccgc aaaaccaatc cctgtcctgc tcaggtcaag ctgtatgcgc gggtcttcct
    13441 tcacgaacaa tccattttgc acagatactt tccaaatacc ggaatactcc aatacccggg
    13501 ctatctgatc ttctgtaacg ttcttcgtgt tgccgggatt gtttccccaa agctcacgca
    13561 acggtcctgt ggtggtgttc atggatgcac ccgtaagttc tgctgtggct tttgtcttgt
    13621 caagcactgc agcaaagatg gagtcggttt ccatactttt gttggtgttc acactgttga
    13681 tgatagaaga agggttatac agggaaattg agcggtcgat ctcgcgcgcg catttcctgg
    13741 cagccttttt aatatccgat ttgaaagacc agggtgcata atcaggggcc tggtaaacaa
    13801 tctggtaaga ataacccatg accacccctt tgatggtttg atattcttgt tccggagtac
    13861 agcccgcaat aacaagcagg catatcaggg tagccattga cgggaatagt attcgtctca
    13921 taattcaata atgatattat cggtggcata cgtcccgaat accccgtttt ggtcgtatat
    13981 caggtatgct ccca
//
LOCUS       DAOSOF010000043        13742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4486_length_13742_cov_4.671732, whole genome shotgun sequence.
ACCESSION   DAOSOF010000043 DAOSOF010000000
VERSION     DAOSOF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4486_length_13742_cov_4.671732"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1254)
                     /locus_tag="PLM68_03840"
     CDS             complement(178..1254)
                     /locus_tag="PLM68_03840"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOT54530.1"
                     /translation="MKHFAFLIAAMTLIGSCSSGPDELTLLVGTYTTGNSYGIYSCKF
                     SLQTGELVLLDSTAADNPSFLTVAAGKSTVYAVHESGPASAVSAYAPGGENGGLQRLW
                     RIENPGSDPCHVAADPAGAYVVTADYSSGTVTFYGMEGNGIVQQYAFTGSGPDERRQR
                     SSHVHCVVFSPDGEHLFATDLGADRIYVFNKDSADIPLLKDTVDLESGSGPRHLVFNN
                     SGNRAYLINELSGMVSVFEHDKGKLKQIQSIEADTCKARGSAHIALSGDGRFLYVSTR
                     LQGDGLVIFSVDPAYGMLTKAGYQPTGSHPRHFLITPDDHWVLVACKNENHIEIYSRD
                     VTSGLLRDTGKRMRLEQPVCIGAL"
     gene            1842..2009
                     /locus_tag="PLM68_03845"
     CDS             1842..2009
                     /locus_tag="PLM68_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54531.1"
                     /translation="MKISLEGHRAPFGAGIRINGKVASTTRVGKPRLKEKASRPCGDS
                     DRLQERIEASA"
     gene            complement(2128..2868)
                     /locus_tag="PLM68_03850"
     CDS             complement(2128..2868)
                     /locus_tag="PLM68_03850"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like C-terminal domain-containing protein"
                     /protein_id="HOT54532.1"
                     /translation="MKPTSKLTLLITAAALLMTLGSCSKWLNPDENTRVVWATIITTY
                     DMPAGEEYPYYFITDDSLLYCPMNHASIDDDFEPVDGQRLILYYKFHGTSTQEESDEE
                     QTLNPGEIKYIDIIQMQMVDTKNFVFTYEPDTLGTDRVEPQYLWLAGGGLGTKRYLNL
                     QLYIQAFTPDKHTFAMAYDLNRETPLEDGYYCLEFQHDANEDPMYSNYTTLISYPLEG
                     PCIEEGVKGLKIKVNTINQGLKVYTIDY"
     gene            3005..3523
                     /locus_tag="PLM68_03855"
     CDS             3005..3523
                     /locus_tag="PLM68_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54533.1"
                     /translation="MKDRELWKEIYEKYAKRMLTVCLRYSPGREEAEDFMHEGFMQVF
                     RSLDKFTDRGEGSLRAWMERIMINTCLQGYRKKDVLRDSKPVETVPDTEDDSYLNEAA
                     ERVPIDVLQRFIGDLPQGYRTVFNLYVFEGLSHKEIAQQLGIRERTSSSQFYRAKCLL
                     VRLIKEYERKQR"
     gene            3504..4697
                     /locus_tag="PLM68_03860"
     CDS             3504..4697
                     /locus_tag="PLM68_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54534.1"
                     /translation="MKGNNDKWIQTMRHRMDQAPVSFDAGGSWIRMEQKIRSFYRIRL
                     WRRIGLSAACAAAIIVPLLFLSREEATERIRPVQLLELRYTASTLSYPAERPRRLIEA
                     DKDHHTAPVTVVPAGEIVMEEPAQQKDVKAANAEQQEPQAATPKMAKPGTPANLFIED
                     VFEVKKRSRMMLAASGVLAVRETRKMSSLPPGDAFMDENKLMSTGILQNNETLNSYEA
                     TILPVNYVTYEYKHRLPVSFGLVASFPLSERWYVESGLFATRLASSIYSVHSMRQDDP
                     VFITDRVLWYLGVPLKIRWNFLQSRVFTAYAAAGGALEKCIYFGYEQAMEDEKMQLTP
                     RDMPVMWSVQATLGAQYNIVKGVGLFVEPGISFYPENSKMPVKTIRTDKPLNFNLNAG
                     FRFSF"
     gene            4775..4966
                     /locus_tag="PLM68_03865"
     CDS             4775..4966
                     /locus_tag="PLM68_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT54535.1"
                     /translation="MNNGTVKFYDEIKGFGFIRDSKTSKEYFVHSSGLIDRIRDNDEV
                     TFDLQEGRKGLNAVNVRRS"
     gene            5031..5606
                     /locus_tag="PLM68_03870"
     CDS             5031..5606
                     /locus_tag="PLM68_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOT54536.1"
                     /translation="MIYLLAHVDNLPESFPDTCTTFMPAERTRQMMCYNRTQDRKLCA
                     AAYVLLVYALKTEKLFNGLPIFAYGPCNKPFLANYPGIHFNISHCEGTVVCALSNTPV
                     GIDIEKVIPYDDDLARYICNTSEYQWINAAPEEKSLRLTEMWTRKESGVKCSGTGIDC
                     HPSTMESGLSRVIHAKTKNDLYIISIRLCNN"
     gene            5594..12910
                     /locus_tag="PLM68_03875"
     CDS             5594..12910
                     /locus_tag="PLM68_03875"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOT54537.1"
                     /translation="MQQLTYPLTSAQMGMYYEWKKDKEQTRYNNPFLYEFPETIDTER
                     LKDAYVKLIEAHSFLTLRLKTLGNGDVVQYFVPDVPVNIPVIETVAEGLRERVLSGIR
                     PFDLLAGEPLYRIVIYKTPFRVYVFMDIHHIAYDGSSSIVFHRDLAKAYNGETLSKET
                     LTCAQFALLEAERMRGEEYKRDEAYFREKLSGITPAKLPILKRSEEAAGTLDKVSEYV
                     DYKQIIDYCKRLEISPNNLFAGALGICLNQYTREKKLCFCTAHNGRIDERVRESVGMF
                     VKTLPVVVVPDPLLSLADYLQGIRSDMKELWLHQTYPFSEMATHYGVAMDLTYTFQKG
                     ILEYFEMPQGNVRLEYLHERSTYDKLSVYIYQFPDNFEIRCEYNNSLYDRDYMEVFAA
                     SLKNALLEILAADPVQATCSQINITNAAEKEKVLEFARGTTLPYDTTKTFIDLFRDMV
                     ARYPEYPSVKDINGSLTYAQLDCQSDAMAHKLMAMGVKPNDFVSVVLSRRKEFVVAVL
                     GIQKARAAYVPMDSEYPIDRLLYMLEDSSSAVLVTEREMYAKKQEEGDFHHHNILFLE
                     DVKLDGPAEKITSLPQPGNLAYMIYTSGSTGKPKGVMISHMGLAAMCIGHAHDMDIIP
                     GDNNLCHASFSFDASVHDLFPPLTAGACVHVIPADMRQDMIMLYDYILANHITGSTIS
                     TQFGLEMINQFELPMKYMLVGGEKMFPVKKRNLKIINGYGPTEFTVCSDYHIVDQEKD
                     TKNIPIGRPVPNSWSYIVDDRQQLLPAGVAGELCLAGPQIAMGYWQREELTAERFLPN
                     PYSSRPINEKLYRTGDLVRWRSDGLIEYLGRIDTQVKLRGFRIELGEVETVLSKFPGI
                     VSAVAEVREFGGAQQLCAYYQTEGDVAIDEEALDEHLRASLTEYMVPSALLKLKAFPM
                     TPNGKVDRKKLPIPQLTIKEIVLPQTIVEKDLFAIVSDILQTDAFGVTTNLLSIGLTS
                     LLAIRLSVKVKQELDKNLPTKDILKLKTIRLLARLLTREEQEPGISGITTYPAQEYYP
                     LTETQKGIYYDWEKARDALQYNIPIAIRSADPDTPDPSRLREALLAVMEMHPYLKTRL
                     VLVGEEVMQQRRDGQAVDILVKNEDEDKMKSIMTEFVQPFDLFHDDMYRFEIYTTQSA
                     VYLLMDLHHIAFDGVSMGIFLQDLKHALEGKDLSPEAVSAFDVSLYEQETLKSPAYQK
                     AQDYFDTLTGGECSMSVIPPSPKVPEGKENGNYNQTVPGEAIDTFCRNYGLTANTFFL
                     SALTQAVQRYTREEQVVLTVSSGGRADPQLARTVGMFVKTLPVKTDMKPRPFIEFSKD
                     IQEQMFSTMEHEIFPFTRMVDKYGIVPQINYVYEGGLGIEMNVDSKPLQIIFLDLEAP
                     RFPFGVVVEPQDNSYVISIQYDNTKYAHEDVVRFLELYKNIASHAALKPDTLTSELTL
                     MVPSVRQQVMDFSEGTQLPYNTSKTFIDLFRENAHKYPDNKAVADKNSSLTYRLLDTY
                     SDRLAAELATLGVAPGTFVAVMMSRRKEFVVSIVAIQKAGGAYVPMDSEYPNDRLLYM
                     LENSESKVLITERTIYESKKMEGAFQADRVLFIDEFDFDKERVLNVQPSVPEDIAYMI
                     YTSGSTGKPKGVMICHLSLAAKVHWQVHDLEITPQDANVCHPSFSFDASIDDLFVPLA
                     GGASVHILSEDIRQNMKELYDYLVAQNITGGSFSTQFGLEMANQFELPLRYMIMGGEK
                     MVPVKKRNVQIINGYGPTEFTVCSSYHKVDQARDTENIPIGRPVPNTWSYVLDSRQQL
                     LPPCIPGELCLAGRQTALGYWKREDLTRERFIPNPYATSQDNGILYRTGDLVRWNANG
                     ELEYMGRIDNQIKLRGFRIELGEIETMMSGYKGIASAVADVKEFGGAQQLVGYYVVES
                     FGKVDPAALEAYLRSSLTEYMVPSAFVEMKTFPLTPNGKVNRKLLPLPEMLRQHPYVA
                     PGNELEEELCKIYANILQLEKVGILDDFFHIGGTSMSAIKAIIRIINLGYDIKYGDLF
                     EHKTPQAIAAFLTESAMSKAKEKKHAGKKGIDDYDYSAINHLLDKARPQLWDGYKPVE
                     VGEVLLTGATGYLGMHLLKELIEHEDNVIYCMVRSKGVLTPERRLKSQLMYYFSDTYD
                     ELFGKRIIPVDGDITNTGTLESLKGLGISTVYNCAAVVKHYAAGNELEKINVEGVSNL
                     VEFCRSDNARLIHISTVSISGMLSRGTEEGKILDEGALYIGQEIENQYVLSKFLSERI
                     VLEAVSAGVINGKIMRVGNLMGRHSDGEFQINFRSNAFINSLRSYQVLGMFPLSGLIA
                     PAEVSPIDCVARAVHVLSKTPSEVVVLHAYNNYRLNMANLVYAMREYGFDIELVSDER
                     FNHHFNEMMKDPSKSEYLGGLLHYGTDTEKVPVPDDNSYTTLLLYRNNVRWPLADEGY
                     SLKLIEMLDGMGFFVS"
     gene            complement(12917..>13742)
                     /locus_tag="PLM68_03880"
     CDS             complement(12917..>13742)
                     /locus_tag="PLM68_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT54538.1"
                     /translation="SNVIMQRDGTRYLSVLLVPGGISEYYREANQRGNVKTVWYDSPT
                     IGMQRRMTVYTPYGYENNPRAKYPVLYLLHGAGGDEEAWISMGYTRQIMDNMIEKGEA
                     EPMIVVMPNGNPWQEAAKTYGLPERQFDWRDPAFANLYVNSIVKDIIPYVEKHFRTIN
                     KPEGRAVAGLSMGGGHTMAVTNLYPGMFGYICPLSMGIREGQEIDSQLQAIKKAGYKL
                     YWVGCGDQDFLFESANALHQALERNGLEHTYHVSGGGHTWPNWRTYLTIFGKLLFK"
BASE COUNT         3684 a         3264 c         3388 g         3406 t
ORIGIN      
        1 aagaaaacct gcgatcaatc gctgcccctg ctttcacgca ggaagagttg ttgcttctgg
       61 aagcataaaa aacactggat gaaggaacct tcatcaaaca caaggaacct gctttcctgg
      121 tactataatc ccggcctgat attactacgc cttaacctga tagtataccg cctgatatca
      181 caacgccccg atgcaaacag gttgttccag cctcatacgt ttcccggtat ccctgagcag
      241 tcccgatgtt acatcccggc tgtaaatttc tatatggttt tcattcttgc aggccaccag
      301 cacccaatgg tcatcaggtg taatgaggaa atgcctggga tggcttcccg tgggctggta
      361 tcctgctttg gtcagcatgc cgtatgccgg atccacgctg aaaatgacca gcccatctcc
      421 ctgcaaccgt gtggaaacat aaaggaaccg gccatcgccc gaaagggcta tgtgggcact
      481 cccgcgggcc ttgcaggtgt ctgcctctat tgattgtatc tgttttaatt tgcctttgtc
      541 gtgctcaaaa acactaacca ttcccgaaag ttcgtttata aggtacgctc tgttgcctga
      601 attgttgaat accaggtgtc ttggaccgct tcccgattca aggtccactg tatctttgag
      661 taggggaata tctgctgaat ccttgttaaa gacataaatc cggtcagctc ccagatcggt
      721 tgcaaaaagg tgttcgccat cgggagaaaa aaccacacaa tggacgtgtg atgatcgttg
      781 tcttcgctca tcgggtcctg atccggtgaa agcatattgc tgtacaatac cgttgccttc
      841 cattccatag aaagtgaccg tgccggaaga atagtctgcc gtgaccacat aagctcctgc
      901 cggatctgca gccacatgac aggggtccga tccgggattt tctatgcgcc agagccgttg
      961 caggccgccg ttttcacccc ctggagcata agcagataca gcagatgcag gaccactttc
     1021 gtgaacagcg tataccgtgc ttttacccgc tgccactgtc agaaacgaag ggttgtccgc
     1081 agcagtgctg tcaagcaata caagctcacc cgtttggagg gagaatttgc aggaatagat
     1141 tccgtaacta ttcccggtgg tataagtgcc taccagcagt gtcagttcat ccggtcccga
     1201 actgcaagag ccaatcaaag tcatggctgc aatcaaaaag gcaaagtgct tcataatcgt
     1261 attttctaca aaaataaaaa aactccgctc aaataaggag gtggaacctc ccgcgccatt
     1321 tttgacgtag caaatttgta tgtaactgta ttacagcaat ataagaaatt actattttaa
     1381 atttggcgca tgaggttcca cctccttatt gaggtttgct ggaggtggta agtagtgaaa
     1441 aacgcgaccg cgcctctaac gtgcacttaa acagtacatt gtgcgttttt gtaacataaa
     1501 tgttacttac cacctccctg caaaaccgct gaaatccagg gaggtggtaa gttgtaaaaa
     1561 ttgcgctcat gcctataaaa cacacaataa cagcggatta tgggcttttg catcaaaacc
     1621 attacttacc acctcccccc aaaaacacca gaaaaccgca aaaaaagggg gagatggtaa
     1681 gtagtgaaaa acgcgaccgc gcctctaacg tgcacttaaa cagtacattg tgcgtttttg
     1741 taacataaat gttacttacc acctcccgta tagttaccgc aaaaaatgat tggtgctgcg
     1801 gagcagcata tgtaagcgct aaacgctttt ggcgcaccgc cttgaagatc agccttgaag
     1861 gtcaccgggc gccttttggt gcaggaatac gcattaacgg caaggtcgct tccaccacaa
     1921 gggttgggaa gcctcggctt aaagaaaaag cttcccggcc ttgtggtgac agcgaccgtc
     1981 ttcaggaaag gattgaagcc tcggcttaaa gaaaaagtgc ttcgtacttt acagtacagg
     2041 gacggtttgt gccagggatg gtggaataaa aaaaccccgc tcaaaaaagc ggggaggagc
     2101 aaaactgcat ttaaccgtaa aagcaaatca ataatcgatc gtatagacct tgagaccttg
     2161 gtttatggtg tttaccttga tcttcaagcc ttttacgcct tcctctatac agggtccttc
     2221 aagaggataa gatatcaggg tagtgtaatt gctgtacatg ggatcttcat tggcatcgtg
     2281 ttgaaattcc agacaatagt agccatcttc caggggtgtc tcccggttca ggtcataagc
     2341 catggcgaag gtatgcttat ccggggtgaa tgcctgtatg tacagttgaa gattcaggta
     2401 gcgttttgtt cccagacctc ctcctgccag ccatagatat tgcggctcga ccctgtcagt
     2461 accaagggta tccggctcat aagtaaaaac gaaattcttt gtgtcgacca tttgcatctg
     2521 gataatatca atgtacttga tttctccggg gttaagtgtt tgttcttcat cactttcttc
     2581 ctgggtgctt gtgccgtgga atttgtaata caggatgagt cgctgaccgt ctacaggttc
     2641 aaaatcgtcg tcgatagagg cgtggttcat tggacagtac agcaagctgt catcggtgat
     2701 gaaataatag gggtattctt cacctgccgg catatcatag gtggttataa tggttgccca
     2761 tacaactctt gtattttcat ccggattcag ccatttgctg caggaaccca aagtcatgag
     2821 cagggcggcc gcagtgatta ataaggtaag tttagaggtc ggtttcatat ttttgctact
     2881 ttagaatgtt ctttctgtat aataattgca gcatttgttg gtatactgcg tctaagtaag
     2941 gaatattttt tcaataagca gtattgatga tccttttgca tttaatgatt gaccgtaaaa
     3001 actgatgaaa gacagggagc tttggaaaga aatatatgaa aagtacgcaa agcgcatgtt
     3061 gacagtttgt ttgcggtact ccccgggaag ggaagaggca gaggatttca tgcatgaagg
     3121 attcatgcag gtatttcgtt ctctggacaa attcaccgac aggggagaag gttctttgag
     3181 ggcctggatg gaaaggatca tgatcaatac ctgtttgcag ggttaccgca aaaaagatgt
     3241 actcagggat agcaaaccgg tagaaacggt tcccgatact gaggatgact cctatctgaa
     3301 cgaagctgcc gaaagggtcc ctattgatgt gctgcagcgt tttatcgggg atttgccgca
     3361 ggggtaccgt actgttttca atctctatgt attcgaaggc ttgtcccaca aagagattgc
     3421 ccaacagctc ggcatacggg aaagaacctc atcctcacag ttttataggg ctaaatgcct
     3481 gcttgtcagg cttataaaag aatatgaaag gaaacaacga taagtggatc caaaccatgc
     3541 gccaccggat ggaccaggcg ccggtctcct ttgacgccgg tgggtcctgg atacggatgg
     3601 agcagaagat ccgcagcttt tacaggatca ggctttggag aaggattggc ctttcggctg
     3661 cctgtgctgc cgccattatt gtcccgttgc tgtttttgag ccgggaagag gctacagaga
     3721 ggatacgtcc tgttcagctc ttagagctcc gctacacggc aagcaccctg tcctacccgg
     3781 cagaaagacc ccgcaggcta attgaggctg acaaagacca ccacaccgct cctgtaactg
     3841 ttgtaccggc aggggagatt gtcatggaag agccagcgca acagaaggat gtcaaagcag
     3901 caaatgcgga acaacaggaa ccgcaggctg caacacccaa aatggcaaaa ccgggaacac
     3961 ccgcaaatct cttcatagag gatgtttttg aggtgaaaaa gcgctccagg atgatgctcg
     4021 ctgcatctgg ggtgctggcg gtcagagaaa cccggaaaat gtcttcattg cccccgggcg
     4081 atgcgtttat ggacgaaaac aagcttatga gtacaggcat cctgcaaaac aatgagaccc
     4141 ttaacagtta cgaagccacc atactgcctg tgaattatgt cacctatgaa tacaagcacc
     4201 gtttacccgt ctcattcgga cttgttgctt cgttccccct gtctgaacgg tggtatgtgg
     4261 agagcgggct gtttgctaca cgcctggctt cgtccattta ttcggtacat tctatgcgtc
     4321 aggatgaccc ggtatttatc accgaccgtg ttctctggta cctgggtgtg cccctgaaaa
     4381 tacgctggaa ttttctacag agcagggttt ttacagctta tgccgcggca ggcggtgctt
     4441 tggaaaaatg tatttatttt ggttacgaac aggcaatgga ggacgagaaa atgcaactta
     4501 caccacgtga catgcccgta atgtggtcgg ttcaagctac tttgggagcc cagtacaaca
     4561 ttgttaaagg ggtagggctg ttcgtggaac ccggaatatc cttttacccc gaaaatagca
     4621 aaatgccggt gaaaaccata cgcacggaca agccgttgaa ttttaaccta aatgccggct
     4681 ttcgtttttc tttttaatgt ttttttttag cttttccgca attatccaat atattatcat
     4741 acatttgcac atcaattatt tttatttttt ttcaatgaat aacggaaccg ttaaatttta
     4801 cgatgaaatc aaaggttttg gttttatcag ggactcaaag acgtccaaag agtattttgt
     4861 acattcatct ggcctgattg acaggatcag ggataatgat gaggtgactt tcgacttgca
     4921 agaaggcaga aagggattga acgcagtcaa tgtaagacgc agctaatcct gcaacatact
     4981 atgatgcatc ccgctgtgaa gcacggcggg atttttttta catttgtatc atgatctacc
     5041 tgctggccca cgtcgataac ctaccggagt cttttcccga tacctgcact acttttatgc
     5101 ccgctgaaag aacaagacaa atgatgtgct acaacaggac acaagatcga aaactttgtg
     5161 ctgcagccta tgtgcttctg gtctatgcac tcaaaacgga aaagcttttc aacggtttgc
     5221 ccatttttgc ctatggcccc tgcaacaaac cgttcctggc caattatccg ggaattcatt
     5281 tcaacatcag ccattgtgaa gggactgtag tgtgcgcgct gtcgaacact ccggtaggca
     5341 ttgatattga aaaagtcata ccctatgatg atgatctggc ccgttatatt tgtaatacaa
     5401 gtgaatacca atggattaac gctgctccgg aagaaaagtc cctacgtctg acagaaatgt
     5461 ggacccggaa agaaagcggg gtcaaatgtt cgggaaccgg catagattgt cacccctcga
     5521 ccatggaaag cggcctcagc cgggtaattc acgctaaaac taaaaatgat ctttatataa
     5581 taagcataag actatgcaac aattgacgta tcccctgaca agcgcccaga tgggcatgta
     5641 ttatgagtgg aagaaagaca aagaacagac ccggtacaac aatcctttcc tatacgaatt
     5701 tcctgaaaca attgatacag agcgcctgaa ggatgcttat gtaaaactca tagaagccca
     5761 ttcgttcctc acactcaggc tgaaaacctt gggtaacggg gatgtggtgc agtattttgt
     5821 cccggatgtt ccggtaaata tcccggttat tgaaacagtt gcggaagggc tgcgtgaacg
     5881 cgtcctctct ggaatccgtc cgttcgatct tttggcggga gagcccctgt accgcatagt
     5941 gatttataag acacccttcc gtgtctatgt ctttatggat atacaccata ttgcttatga
     6001 cgggagttca agcattgtgt ttcaccgtga cctggcaaag gcctataatg gggaaactct
     6061 gtcaaaggaa accttgacct gtgcgcaatt tgccctgctg gaagcagaac gcatgcgcgg
     6121 agaagaatac aaacgggacg aagcctattt cagggaaaag ctttccggga taaccccggc
     6181 caaattgccc atactcaagc gttcggaaga ggcagccgga acgctggata aagtatcaga
     6241 atatgtcgac tataagcaga taatcgatta ttgcaaacgc ctggaaatat ctcccaacaa
     6301 cttgtttgcc ggagccttgg gaatctgcct gaaccaatat acacgggaaa aaaagttatg
     6361 tttctgtacg gcgcacaacg gtaggatcga tgaacgggtg cgcgagagtg tggggatgtt
     6421 cgtaaaaaca ttgcctgtag tggttgttcc tgacccgctg cttagtctgg ctgattacct
     6481 gcagggtatc cggtcagaca tgaaagaact ctggttacat cagacgtatc cgttttcaga
     6541 aatggcgaca cattacggtg ttgccatgga tttgacctat accttccaga aagggatcct
     6601 ggaatatttt gaaatgccac agggtaatgt ccgtcttgaa tacctccacg aaagatcgac
     6661 atacgacaaa ctgagtgtct atatttacca gttccccgat aatttcgaga tccgctgtga
     6721 atacaacaac tcactctacg atagggatta catggaagtc tttgccgctt ccctgaaaaa
     6781 tgccttgctg gagatcctgg ctgcagatcc tgtacaggct acctgttcac aaatcaacat
     6841 caccaatgcc gctgaaaagg aaaaagtgct ggaatttgcc cggggcacaa cattgcctta
     6901 cgatacaacc aaaactttta ttgacctgtt cagggatatg gttgccaggt atccggaata
     6961 tccctctgta aaagacatca atggctcatt gacatacgcg caactggact gccagtcaga
     7021 tgcgatggcc cataaactga tggccatggg cgtaaaacca aatgactttg tatctgttgt
     7081 gctttcaagg cgtaaagaat ttgtggtggc tgtgctgggt attcaaaaag ccagggccgc
     7141 ttacgttcct atggacagcg aatatcctat tgaccggttg ctttacatgt tggaagacag
     7201 cagttctgcc gtgcttgtca cggaacggga aatgtatgcc aaaaagcagg aagaagggga
     7261 ttttcatcac cataacatac ttttcctgga agacgtaaaa ctggatgggc ctgctgaaaa
     7321 aatcaccagt ctgccccaac ccggcaacct ggcttacatg atttatacct cgggctctac
     7381 cgggaagccc aagggggtga tgatcagcca catgggactg gcagccatgt gcatcggcca
     7441 tgcacacgat atggatatta taccgggtga taacaacctc tgccatgcca gcttcagctt
     7501 tgacgcctcg gtgcatgatc tatttccacc actcacggct ggtgcatgcg tacatgtgat
     7561 ccccgccgat atgcgccagg acatgataat gctctacgat tacatcctgg ccaaccacat
     7621 caccgggagc accatcagca cacaattcgg gctggaaatg atcaatcagt ttgaacttcc
     7681 tatgaaatac atgctggttg gcggggagaa aatgtttccc gtaaagaaaa ggaacctgaa
     7741 gattattaac ggttacgggc ccactgaatt tacagtgtgc tctgattatc acatagttga
     7801 ccaggaaaaa gatactaaaa atattcccat tggacggcct gtccccaatt cctggtcgta
     7861 tatagtggat gacagacagc aattgctccc ggcaggagta gccggtgagt tgtgtctggc
     7921 aggaccgcag atagccatgg gttattggca gcgggaagag ctgacggcag aaaggttcct
     7981 gcccaatcca tatagcagca ggccaatcaa cgaaaaactc taccgcacag gggacctggt
     8041 tcgctggcga agcgatggcc ttatagaata cctgggccgc attgacaccc aggttaaatt
     8101 gcggggattc cgcatcgaat tgggtgaggt agaaaccgtc ctttccaaat tcccgggaat
     8161 tgtttcggcc gttgccgagg taagggaatt cggaggggca cagcaactgt gcgcctatta
     8221 ccagacagaa ggtgatgtcg caatagacga ggaagccctg gacgaacacc tgcgtgccag
     8281 cctgactgaa tacatggttc cctcggcctt actaaagctg aaagcctttc ccatgacccc
     8341 taacggaaag gtggaccgca aaaaactacc cataccgcaa ctgaccataa aagagatcgt
     8401 actaccgcag acaatcgtgg aaaaagacct ctttgccatt gtctcggata ttttgcagac
     8461 agatgccttt ggggtgacca ccaacctgct gtccattggc ctgacctcgc tgctggccat
     8521 ccgcctatcg gtcaaggtga aacaggaact ggacaaaaac ctgcctacca aagatatcct
     8581 caaattgaag accatccgcc tgctggcccg gctcctgaca cgggaagagc aggagccggg
     8641 aatctcaggc ataacaacat atcctgcaca ggaatattac ccattgaccg agacacagaa
     8701 aggcatatac tatgactggg aaaaagcccg ggatgccctg caatacaata ttcccatagc
     8761 catccgttca gcggatcccg acaccccgga tccttcacgg ctcagggaag ccctgcttgc
     8821 tgtcatggaa atgcatccgt atctgaaaac ccgcctggta cttgttgggg aagaggtaat
     8881 gcaacagcgg cgtgacgggc aggctgtgga catactggtc aaaaatgaag acgaagacaa
     8941 gatgaagtcc atcatgacgg aatttgtgca accttttgac ctgttccatg atgatatgta
     9001 ccgctttgaa atatacacca ctcaatctgc cgtctacctg ctgatggatt tacaccatat
     9061 agcttttgac ggtgtctcca tgggcatttt tctgcaggat ctgaagcatg cactggaagg
     9121 aaaagatttg agccccgagg cggtttcggc ctttgatgta tctctttatg agcaggaaac
     9181 cctgaaatcc ccggcttacc aaaaggcgca ggattatttt gacaccctta ccggaggtga
     9241 atgcagcatg tctgtgatcc ctccttcacc aaaggtgccg gaaggaaagg aaaacgggaa
     9301 ttacaaccaa accgttccag gagaggctat agatactttc tgccgtaatt acggtctgac
     9361 ggccaatacc tttttccttt cggcccttac ccaggccgta cagcgttata cacgcgagga
     9421 acaggtagtg ctgaccgttt cttcaggtgg cagggcagat cctcaactcg cccgtaccgt
     9481 gggcatgttc gtcaaaacac tacctgtgaa aacggacatg aagccccggc ctttcataga
     9541 attttccaaa gatatccagg aacagatgtt cagtaccatg gagcacgaga tctttccctt
     9601 caccaggatg gtggataaat acgggatcgt accccaaatc aattacgtat acgaaggggg
     9661 attgggcatt gaaatgaatg ttgacagcaa acctttgcaa attatattcc tggatctgga
     9721 agctccaaga ttcccctttg gtgtggtcgt ggaaccacag gataacagtt acgtgatttc
     9781 cattcaatac gataacacca aatacgccca cgaagatgtg gtgcgctttc tggaactata
     9841 taaaaacatt gcctcccatg ctgccctgaa gcccgatacc ctgacttcag aactcacact
     9901 tatggtaccg tctgtaaggc aacaagtgat ggatttttct gaagggacac agcttccata
     9961 caatacttca aaaacgttca ttgacctgtt ccgggagaat gcccataaat atcctgataa
    10021 taaagccgtt gcagataaaa acagttcact tacataccgt ctgctggaca cgtattcaga
    10081 ccggctggct gcagaactgg caaccctggg agtagccccg ggaaccttcg tcgccgtgat
    10141 gatgtcccgg cgtaaagaat tcgtggttag cattgtagcc atacagaaag caggaggggc
    10201 ttacgtgccc atggacagtg aataccccaa tgaccggctg ctgtacatgc tggaaaacag
    10261 cgaatccaaa gtgctgatta cagaaaggac aatttacgaa tccaagaaaa tggaaggggc
    10321 ttttcaagct gaccgggttc tttttattga cgaatttgat ttcgataagg aacgtgtgtt
    10381 gaacgtgcaa ccatctgtac cggaagatat tgcctacatg atctatactt ccggttcaac
    10441 tggaaaacct aaaggggtga tgatctgtca cctttcgctg gcggccaagg ttcactggca
    10501 ggtacacgat ttggaaatta ccccgcagga tgccaatgtt tgtcatccca gtttcagctt
    10561 tgatgcttcc atagacgatc tgtttgtacc actggcaggg ggcgcttccg tacatattct
    10621 gtccgaagat atccgacaga atatgaagga actctatgat tatttggtgg cccaaaacat
    10681 taccggggga tctttcagca cccagtttgg cctggagatg gccaaccagt ttgagctgcc
    10741 ccttagatac atgatcatgg gaggcgaaaa aatggttccg gtgaaaaaac gtaacgtaca
    10801 aataatcaac ggatacgggc ctactgaatt tactgtctgc tccagttacc acaaggtaga
    10861 tcaggccagg gacacggaaa acattcctat tggcaggcct gttcccaata cctggtctta
    10921 tgtgctggat tcccggcagc aactgctgcc cccctgcatt cccggagaat tgtgccttgc
    10981 aggccgtcag acagctctgg gatactggaa gcgggaagat cttacccggg aacgttttat
    11041 tcccaatccg tatgcaacat ctcaggataa cgggatcctt taccgtaccg gcgatctggt
    11101 gcgatggaat gcaaatggtg aattggagta catgggtcgc attgacaacc agatcaaact
    11161 gaggggattc cgcattgaac tgggcgagat agaaaccatg atgtccggtt acaaaggcat
    11221 tgcctcggca gtggccgatg tcaaagaatt cggcggggca cagcagctgg tgggttatta
    11281 tgttgtggaa tcctttggaa aagtggatcc ggcggcgctg gaagcttacc tgcggtcctc
    11341 tctgaccgaa tacatggttc cctcggcgtt tgtggaaatg aagacttttc ccctgacgcc
    11401 caacggcaag gtgaaccgca aattgctgcc cctgcctgaa atgctgcggc aacatcccta
    11461 tgtggccccg ggcaacgaac tggaagaaga actgtgtaag atctacgcca atatactgca
    11521 attggaaaaa gtggggatac tggacgattt tttccacatc gggggcactt ccatgtcggc
    11581 catcaaggcg atcatccgca tcatcaacct gggctacgac atcaagtacg gggatctatt
    11641 tgagcacaag acaccgcagg ccattgccgc gttcttaacc gaatcggcca tgtccaaagc
    11701 caaagagaag aaacacgcag gaaaaaaagg gattgacgat tatgattact cggccatcaa
    11761 tcatttgctg gacaaggcgc gtccgcagct ctgggacggc tacaaaccgg tggaagtagg
    11821 cgaggtgctc ctgacagggg ctaccggtta tctgggaatg catctgctga aagagcttat
    11881 agaacatgag gataatgtca tttactgcat ggttcgctcc aagggggtgc tgacgcccga
    11941 aaggcgcctg aaatcgcaat tgatgtatta cttctcggat acctatgatg agctcttcgg
    12001 aaaacgcatc attcctgtgg acggggatat taccaacacc ggcacgctgg aatccctcaa
    12061 aggactgggc ataagcaccg tttacaactg cgccgccgta gtgaagcatt acgctgccgg
    12121 taatgaactg gaaaagatca atgtggaagg ggtgtccaac ctggtggaat tttgccgaag
    12181 cgacaacgca cggctcatcc atatctccac ggtgagtatt agcggaatgc tttccagggg
    12241 cacggaagaa ggtaagatcc ttgacgaggg ggctttgtac attggacagg aaattgaaaa
    12301 ccagtatgtg ctgagtaaat ttctatcgga aaggattgtt ctggaagccg tgtccgcggg
    12361 agttataaac ggcaaaatca tgagggtggg gaacctgatg ggacgccatt cggacggaga
    12421 attccagatc aatttccgga gcaatgcctt catcaattcc ctgcgcagtt atcaggtact
    12481 ggggatgttc ccgttaagcg gactgattgc cccggcagaa gtttcgccca tagactgcgt
    12541 ggcccgtgca gtacatgtgc tttccaaaac gccttcagag gttgtggtgc tgcatgccta
    12601 taacaattac cggctgaaca tggccaacct ggtgtatgca atgagagagt atggctttga
    12661 tattgaactt gtttcggatg aacggttcaa ccatcacttt aatgaaatga tgaaggatcc
    12721 atccaagagc gaatacctgg gtgggttgct ccattatggt acggacacag aaaaagtacc
    12781 cgttccggat gacaacagct acactacgtt attactttac cgcaacaatg tacgctggcc
    12841 gcttgccgat gaagggtact ctctgaaact gatagaaatg ctggacggga tggggttctt
    12901 cgtcagttga acttcattac ttgaaaagca gtttcccgaa aatggtcagg taagttctcc
    12961 aattgggcca ggtatggccg ccgccgctca cgtggtaggt gtgttccaat ccgttgcgtt
    13021 ccagagcctg gtgcaaggca ttggcagatt caaacaggaa atcctggtct ccgcagccta
    13081 cccagtagag cttgtagcct gctttcttaa tagcctgcag ctggctgtca atttcctgtc
    13141 cttcacggat tcccatgctc aaaggacaaa tgtagccgaa catcccagga tacaggttgg
    13201 tcacagccat ggtatggcct ccacccatgg acagccctgc aacggcacgg ccttcgggct
    13261 tgttaatcgt acggaaatgc ttttcaacgt aaggaataat gtcctttact atggaattga
    13321 cataaaggtt ggcaaaggcc gggtctctcc agtcgaattg cctttcgggc aatccgtacg
    13381 ttttggcagc ttcctgccag ggatttccgt tgggcatgac cactatcatg ggttcggcct
    13441 ctcccttttc tatcatgttg tccatgatct ggcgggtgta tcccatgctt atccaggctt
    13501 cctcgtctcc tccggcaccg tgcaacaggt acaggacagg gtatttggcc cgggggttgt
    13561 tctcataccc gtaaggcgtg tacacggtca tcctgcgctg catgccaatg gtgggggaat
    13621 cgtaccatac cgtctttaca ttgcctcttt ggttggcttc cctgtagtat tcagagatac
    13681 caccaggaac cagcaatacg ctcagataac gcgttccgtc gcgctgcatg ataacgttgg
    13741 ag
//
LOCUS       DAOSOF010000044        13620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4534_length_13620_cov_5.235533, whole genome shotgun sequence.
ACCESSION   DAOSOF010000044 DAOSOF010000000
VERSION     DAOSOF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_13620_cov_5.235533"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..3655)
                     /gene="nifJ"
                     /locus_tag="PLM68_03885"
     CDS             complement(131..3655)
                     /gene="nifJ"
                     /locus_tag="PLM68_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT54539.1"
                     /translation="MAKEKKFITCDGNYAAAHIAYLFSEVAAIYPITPSSTMAEYADE
                     WAAHGKKNLFGEPLKIVEMQSEGGAAGAVHGVLQSGALATTFTASQGLLLMIPNMYKI
                     SGELLPTVFHVSARSLAAQALSIFGDHADVMACRQTGFALLASGGVQEAMDMAAVAHL
                     STLKSRVPFLHFFDGFRTSHEIQKIEMLDSGDLKKMISSKSLAAFRERALNPNQPVTR
                     GTAQNPDVYFQAREASNPFYDAVPDIVARYMGEISELTGRSYLPFTYYGDENADRIII
                     AMGSVCDTIKETVDHLNARGERTGMITVHLYRPFSAKYFLRVMPKSVKSIAVLDRTKE
                     PGALGEPLYMDVKNIFAEDPNAPVIVGGRYGLSSKDTTPAQIMSVFENLGRKEAKNNF
                     TIGIIDDVTFRSLPMKEEISVAKPGTYECKFFGLGSDGTVGANKNTIKIIGETTPKYC
                     QAYFQYDSKKSGGFTCSHLRFGDKPINSPYLVTTPDFVACHVPSYLHKYDILKGIKKG
                     GTFLLNSLWDAEETKKQLPDFVKRTLVEKNINFYIINATKIAEEIGLGGRTNTILQSA
                     FFKIAGVIPYDLAVAEMKDAIVKSYGKKGEEIVSMNYAAVDRGGEIEKVTIDPQWKTR
                     SAKFEADTHKRLAPEWVKHVADVVNAQNGDDLPVSVFKGYEDGTMPAGAAAYEKRGIA
                     IFIPEWTPDNCIQCNQCAYVCPHAAIRPFVMTDEEAAKAPESIKRTAGNGPLKGYHFT
                     IQVSPYDCAGCGNCADVCPAKTKALAMKPAGTQFPEQQNYDYLHANVGYKDTIVPKSQ
                     NVKNLGFTQPLFEFSGACSGCGETPYTKVLTQLFGDHMMVANATGCSSIYSASFPSSP
                     YCKDRNGRGPAWSNSLFEDNAEFGLGMRLGSEQIREKLANLIASAQEKDGVTDELKSL
                     FAEYLPVRNNFEQAVALVEKMTPLLKGSKLQPLREIYDLRGYLTPRSQWIIGGDGWAY
                     DIGYGGLDHVLASGENVNVLVLDTEVYSNTGGQSSKSTPAGAVAKFASSGKKIRKKDL
                     GMMAMSYGYVYVAQVAMGANNAQLLKALKEAEAYDGPSLIIAYSPCINHGLSRGMGQS
                     QMEEKLAVECGYWQLYRYNPSLIDEGKNPFTLDSKEPDWSKFQDFIKGEVRYSSLLQT
                     FPDQAAELFVLTEKHAKIRYNNYKRLAQ"
     gene            complement(3683..4780)
                     /locus_tag="PLM68_03890"
     CDS             complement(3683..4780)
                     /locus_tag="PLM68_03890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT54540.1"
                     /translation="MGDAQLIVTHFCIPEHVMCVAALGEGFINKTFLVKTSARQQYIL
                     QCKNRSVFPNIPAMMENISKVTHHILSEGGNTFRLVAAKDGKWYHKDTDGEYWACFTY
                     IPDTYTFEHAVDIDLVRAGGQGVGLFNSQMAGFTGFLEDTLPGFHNISYRFEQFAGSL
                     KRAQSRSSDANESNGISHLEEVSSLVEEVYSRKEQMEAFWELVRSGTLPRRVSHNDTK
                     LSNFLFDKDRQVVCAIDLDTVMQTTVLFDFGDAIRSYANPFPEDHPLLQEVKMDLERF
                     SAFSQGYLCKAAWFLNKEEKTHLAFSALYITYEQYLRFLMDYMDGDTYYRTRYPGHNL
                     VRARSQLALLKSMEAQLPAMQHVLSEIFNTL"
     gene            complement(4814..5686)
                     /gene="fabD"
                     /locus_tag="PLM68_03895"
     CDS             complement(4814..5686)
                     /gene="fabD"
                     /locus_tag="PLM68_03895"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT54541.1"
                     /translation="MRAYVFPGQGAQFTGMGKDLYDSNPKARALFDRADSILGFSLSK
                     IMFEGGADELKQTKVTQPAIFLHSVAAALCDPDFKPDMVAGHSLGEFSALTAAGALSF
                     EQGLRLVSARALAMQAACEEQPSTMAAVLTLEDEKVEEICATISASGCIVVPANYNCP
                     GQIVISGTVEAVERACVAMKEAGAKRAVVLPVGGAFHSPLMESARKSLEDAIGTAVLV
                     KPVCPVYQNVDGLPHTDPGEIKENLVKQLTSPVRWAQTIQHMVADGARNFTEYGPGTV
                     LMGMIKKIAPELFI"
     gene            5753..5826
                     /locus_tag="PLM68_03900"
     tRNA            5753..5826
                     /locus_tag="PLM68_03900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5787..5789,aa:Arg,seq:ccg)
     gene            complement(6061..6504)
                     /locus_tag="PLM68_03905"
     CDS             complement(6061..6504)
                     /locus_tag="PLM68_03905"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOT54542.1"
                     /translation="MKKLAVLLFLSVYSLTALAQDAGKITGVWWNEDKTTKIQVELKD
                     GKYIGTIIYMIPEKYENGEEPKDDKNPDPALQSRSLIGLRILNGFVYNEKKNEWKDGT
                     IYDPASGKTYDCFARLESNDLMKLRGFVAGMRMLGRTSQWFRTTL"
     gene            complement(6522..8996)
                     /locus_tag="PLM68_03910"
     CDS             complement(6522..8996)
                     /locus_tag="PLM68_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54543.1"
                     /translation="MKRRLAISIGLLSCSMVAFQVALMQILSISQWNHFAYMVISVAM
                     LGFGASGTALAFLRNHLVPKIGSNLAFLMIFTSLSIMGILFFSRTVFGGFDSYLVFYE
                     KRHYLNLICSYLMLFVPFFLGASALGLAYSHYASDIGRLYFADLAGSGMGGLLMLILL
                     WHVSPSVVPFVIACLPLVAGLLIFRHNGKTVAEKTGLLTLALLALVLLVAGFLNPPIL
                     PSSQYKSLSRALLLPDARVVETINSPYGQLKRVSAPALRYAPGLSLGYTGQVPVTEVL
                     FHNGDWLGPLVPMALPEREETVVLSALPGTPDAEGAQESLDGKETGHYLDYSTQALPY
                     VIGTPGKVFVADAATGLQAGYALSRGAKEIVAVEPNSTVVGMVTRNIAVPGEQISFIA
                     GHSRYHVQQDDRYDLILLPETGSFGGSSGILALEEQYLFTLDAFSLLWDRLSPGGMIA
                     ATLWVDYPPRSTFKLLATMAVTAESKGVEDPADHIVAVRSWSTITCVLVKNKVSANNI
                     DKIRTFCQSRQFDPLLYPGIHPEEREVYNMLQDKSFFRTTDLLLTREREKLFREYDFQ
                     IRPATDSRPYFSQFLKLGRLSKMREVFGSASMPFLEVGYLIVLLTMAQIFLASFLFIV
                     LPLIFKKVPGKGKTSLLVFFSFIGIGYMFAEMVFIQQFTLFFGSPLYAASAVLSAMLF
                     FSGTGSACSQKMVTGSPRLRLVAGVIAVLLALLALFLPSLMKATIGNPLPVKVLLCAL
                     VIGPPAFLMGMMFPSAILAIRHVGDGSLIPWAWAVNGSFSVISTALAAVISVEAGYQT
                     VMWVAVAAYLMAGISTRFRLFSIFVK"
     gene            complement(9006..9734)
                     /locus_tag="PLM68_03915"
     CDS             complement(9006..9734)
                     /locus_tag="PLM68_03915"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT54544.1"
                     /translation="MARILKYPLTVLLVTAALPALSAILTGTGEQDFARLRSAMVRDQ
                     IVARGVTHRATLQAMRTVERHLFVDASLSSRAYEDNPLPIGYGQTISQPFIVAYMTSL
                     LDLKPEDRVLEIGTGSGYQAAILARMADSVYTIEIVKELAASAKKRLQDLGYHNVEVV
                     PGDGYYGLPGNAPFQAIVVTAAAEHIPPPLIEQLGEGGKMVIPVGSQFMVQNLILVEK
                     KKGRITTTNLMPVRFVPFTREETR"
     gene            complement(9747..10679)
                     /locus_tag="PLM68_03920"
     CDS             complement(9747..10679)
                     /locus_tag="PLM68_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOT54545.1"
                     /translation="MSRRSDKDQILEIFQSLDFEHIDKHPNILIAAAFWEPERYRAAC
                     TAYCFLRSADDLVDNYKSEHPVIGEQEQAALRNNLDRLTGLLMNNSNMAADSELTATW
                     EKFLIPLWPVEAFTRSMIYDIHHQGFPTFSSFLEYAEGASVAPAGIFVHLCGLRKQND
                     HYLPPVFDARETARPCAIFSYLVHIIRDFQQDRLDNLNYFPSDLLLQYGLSQEQLDTM
                     ARGGTVLPSFRLLVRHYMDVADAYRRETLNMIRKVSPVLDTRYRLSLQIIFDLYLMVF
                     QRIDPENGQFTREELNPRPDEVKSRVFNTLMKFL"
     gene            complement(10672..12165)
                     /gene="crtI"
                     /locus_tag="PLM68_03925"
     CDS             complement(10672..12165)
                     /gene="crtI"
                     /locus_tag="PLM68_03925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOT54546.1"
                     /translation="MNDNNNVVIIGAGTGGIATAIFLARKGYRINVYEQSDTPGGRCS
                     CYIREGHRFDLGATIFLMPDLYRKVFDSLGISFDSAIKSKHLDEIYRLYFADGTTFRF
                     TTDRQVMEKQLEALEKGSYARFQEYVEKGYGFYRIAIDQLLGRNYTSLFQFITWKNIS
                     ILFRIKTLTSHMRYIKRFFNHPHLRTAFTFQNIYVGQNPFTAPALFSMLPAAEISQGS
                     LVAEGGMFSVVESLVKTARELGVTFHYNSPVRKITTSGKKATGIELEDGTAVPADVVV
                     ANADLPYVYRELLEDRCYTAWLDKLRYSCSAISFHWAMDKTYPELTLHNVFMSENYKN
                     NLDHIFKEKKVSDDPSFYVFCPRETDPGAAPQGQDTISVVVPVGHLHKKNKDQWDAIR
                     EQVRKGVLERFEKLGLTDFEQHIKFEKCLMPETWETKFHVTKGSVFGSISHFVLQMGY
                     FRPHNRHRTYRNLYFAGGSTHPGNGVPLVLMSAALTSERIIKEHGHE"
     gene            12235..12756
                     /locus_tag="PLM68_03930"
     CDS             12235..12756
                     /locus_tag="PLM68_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54547.1"
                     /translation="MKRIVLLFLCFVCLNGCDPDSWRPSCAPHAYFHFSYENHAWVSV
                     HSGWIIDENGIIRSYKNPHKWNYPDSLGYITRFELEENLSYCDSVIGKVSAREMSYYN
                     HLVFPASKGSLTKPEPHGADMGIRMYSCYWYDQRKGRYRQVILNQYGDWIRTNENDKA
                     VKIYSWLSRLVTN"
     gene            12790..13392
                     /locus_tag="PLM68_03935"
     CDS             12790..13392
                     /locus_tag="PLM68_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54548.1"
                     /translation="MRLTITHYGAWIALLAFVSCGTPRLGTYSIRLQDAESIPVAPTR
                     DMMLSTAEQPDGTTLYVYENPMFRITWSLTNTRFGFILDNLTADTLFIDWDKASYLDQ
                     DGDTLRLIHDGVDFFLKDSIQEITPLEPLGSLEDFLLPASNIHYDPDNYTLWRINHLF
                     YDRKGNIGQRVSVELPLEINRIIYRYRFRFTIDDWKADSF"
     gene            13440..13515
                     /locus_tag="PLM68_03940"
     tRNA            13440..13515
                     /locus_tag="PLM68_03940"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13475..13477,aa:His,seq:gtg)
BASE COUNT         3340 a         3378 c         3447 g         3455 t
ORIGIN      
        1 cggcttaaag gaaaaagcgc tccgttctta gcagtcaaac gaccgcctgt agtgaaggat
       61 taaagcctcg gcataaaaaa agaggatgac ctttttgtca ccctctcttt taatatattc
      121 aacagaatta ttactgtgcc agccgtttgt aattattgta acggattttg gcatgttttt
      181 ccgtcagtac gaacagttcg gcagcctggt cggggaatgt ctgcagcagc gaactgtatc
      241 gaacctctcc cttgatgaag tcctggaatt tggaccagtc aggctccttg ctgtccaggg
      301 tgaagggatt cttgccctcg tcaatcagtg acggattgta acggtaaagc tgccagtagc
      361 cacattcaac ggccagtttt tcctccatct gtgactgtcc catgccgcgg ctcaggccat
      421 ggttgatgca cggagaataa gcgatgatca gggaaggacc gtcataggcc tcggcttctt
      481 tcaaagcttt gagcaactgg gcgttgttgg cacccatggc gacctgtgcc acatagacgt
      541 aaccatagct catggccatc atgcccaggt ctttcttgcg gatctttttc cctgaagaag
      601 cgaatttggc cactgcgccg gccggtgttg atttggatga ctggcctcct gtgttggaat
      661 acacttccgt atcaaggacc agtacgttga cgttttcccc actggccagg acatggtcaa
      721 gacctccgta gcctatgtca taagcccatc cgtcacctcc gatgatccat tgtgaacggg
      781 gggtcaggta accacgcaaa tcgtaaattt cacgaagagg ttgcagcttg gaacctttga
      841 gcaggggggt catcttttct accaatgcca cagcctgttc aaagttgttg cgtacaggca
      901 ggtactctgc aaagagggac ttcagctcat cggttacgcc gtccttttcc tgtgccgagg
      961 caatcaggtt ggccagtttt tcacggattt gctcggatcc caggcgcata cccagaccga
     1021 attccgcgtt atcctcgaac agtgagtttg accatgccgg gccacgtccg ttgcggtcct
     1081 tgcaataagg tgaggatggg aaagaagcgc tgtatatgga agagcagcct gtggcgttgg
     1141 ccaccatcat atgatctcca aatagctgtg tcagcacttt cgtgtacggg gtttccccgc
     1201 aaccggaaca agctcccgag aactcgaaca ggggctgtgt gaatcccagg ttcttcacgt
     1261 tttgggattt ggggacaatg gtgtctttat atccgacgtt ggcatgcagg tagtcatagt
     1321 tctgctgctc ggggaactgc gttccggcag gtttcatggc cagggctttg gtcttggccg
     1381 ggcatacgtc ggcacagttg ccgcaacccg cacagtcgta aggacttacc tgaatggtga
     1441 aatgatatcc cttaagaggt ccgttgccgg ccgtcctttt gatgctttca ggggctttgg
     1501 cagcttcctc atcggtcatg acaaacgggc gaatggctgc gtggggacat acataggcgc
     1561 actggttgca ctggatgcaa ttatccgggg tccactcggg aatgaagatt gcaataccac
     1621 gtttttcgta ggcggcagca ccggcgggca tggtaccgtc ttcatatcct ttgaatacgc
     1681 ttaccggcag gtcatctccg ttttgggcgt tcacaacatc agccacatgc tttacccatt
     1741 caggggccag gcgcttgtgc gtgtcggcct cgaattttgc ggaacgagtc ttccattgcg
     1801 ggtcaatggt cactttttca atttctccac cgcggtcgac tgcagcataa ttcatggaaa
     1861 cgatctcttc cccttttttg ccataggatt tgacaattgc gtccttcatc tcggcaacag
     1921 ccaggtcata gggtatgacg ccggctattt tgaagaaagc agattgcaaa atggtgttgg
     1981 tgcggcctcc caggccaatt tcctctgcaa tttttgtagc attgatgatg tagaaattga
     2041 tattcttttc taccagtgtc ctctttacaa aatcaggcag ttgtttcttg gtttcttctg
     2101 catcccacag gctgtttagc aggaaagtgc cccctttctt gatccctttc aggatatcat
     2161 acttgtgcag gtaactgggc acgtggcagg caacgaaatc aggggttgtc accaggtaag
     2221 gcgagttgat gggcttgtcc ccgaaacgca ggtgggaaca ggtaaaacca ccggacttct
     2281 tggagtcgta ctggaagtat gcctggcagt attttggagt tgtttccccg atgatcttga
     2341 tggtgttctt attggccccc accgtgccgt ccgatcccag tccaaagaat ttgcattcgt
     2401 atgttccggg tttggcaacg gatatctcct ctttcatggg gagtgacctg aaggtgacat
     2461 cgtctatgat gccgatggtg aaattattct tggcttcctt gcggcccagg ttttcaaaca
     2521 ccgacatgat ctgggcggga gtcgtatcct tagaggacaa tccgtagcgg ccacccacga
     2581 taacgggagc attcgggtct tctgcaaaga tattcttcac gtccatgtag aggggttcgc
     2641 caagggcacc tggttctttg gtgcggtcca gcacagctat actctttacc gatttgggca
     2701 tcacacgcag gaagtacttg gccgagaaag gacggtacag gtgcacggtg atcataccgg
     2761 tgcgttcacc ccgggcgttc aggtggtcca cagtttcctt gatggtgtcg catacagaac
     2821 ccatggcaat gataatacgg tctgcatttt cgtctccata ataggtgaaa ggcaggtatg
     2881 aacgtccggt cagttccgag atttctccca tatagcgggc aacaatgtca ggaactgcat
     2941 catagaaagg attggatgct tctcgggcct ggaaatatac atcagggttc tgggccgtac
     3001 ctcgtgtaac cggctggttg gggttgaggg ccctttcacg gaaagctgcc agggatttgg
     3061 aactgatcat ctttttcagg tcacctgaat ccagcatttc aattttctgg atctcatgag
     3121 atgtccggaa tccgtcaaaa aagtggagga agggcacccg ggatttgagg gttgacaggt
     3181 gcgccactgc tgccatgtcc atagcttcct ggacaccgcc cgaggcaagc aatgcaaagc
     3241 ctgtctggcg gcaagccata acgtcggcat ggtcaccgaa gattgacaag gcctgtgcag
     3301 ccagggaacg ggccgagaca tgaaataccg taggaagcaa ttccccggag atcttgtaca
     3361 tgttcggtat catcagcaac aaaccctgcg aagcggtaaa ggtagtggcc agcgctccgg
     3421 actgcaatac gccgtgaact gcgcctgctg caccgccttc gctttgcatc tcaactatct
     3481 tgagcggttc tccaaaaagg ttctttttcc cgtgggctgc ccactcatcg gcatactcag
     3541 ccatggtgga tgacggagtg atgggataaa tggcagcaac ttcactgaac aggtaagcaa
     3601 tgtgagctgc cgcatagttg ccatcacaag tgatgaattt tttttctttt gccataatga
     3661 tatggaatta aatgtaatgt tattaaaggg tgttaaaaat ttcagataaa acatgttgca
     3721 tggcaggaag ctgtgcttcc atgcttttaa gcagagccag ttgtgagcgg gcccggacca
     3781 ggttgtggcc gggataacgt gtccggtaat acgtgtcacc atccatgtaa tccatcaaaa
     3841 accggaggta ttgttcataa gtaatataca atgcagaaaa ggccaggtgg gttttctcct
     3901 ctttgttcaa aaaccaggcc gctttgcata aataaccctg agaaaaagca gaaaaacgtt
     3961 ccagatccat ttttacctcc tgcaacaggg ggtggtcctc aggaaaagga ttcgcataag
     4021 aacggatagc atcaccaaaa tcaaacaata cggttgtctg catgacagtg tccaggtcaa
     4081 tggcacatac aacctgccgg tctttgtcaa acaggaaatt ggagagtttg gtgtcgttgt
     4141 ggctcacccg gcgcgggagg gtgcctgagc ggaccagttc ccagaatgct tccatttgtt
     4201 ccttcctgct ataaacttcc tccaccagcg atgagacctc ttccagatgg gagataccat
     4261 tgctttcatt cgcgtccgaa gagcggctct gtgctctttt cagcgacccg gcaaattgct
     4321 caaaacggta actgatgttg tgaaagccgg gaagcgtgtc ttccaggaaa ccagtaaaac
     4381 cggccatctg gctattgaaa aggcccacac cctggccacc cgccctgacc aggtcaatat
     4441 ccacagcatg ttcaaaagta tatgtgtccg gtatgtatgt gaaacaggcc cagtactcac
     4501 cgtccgtgtc tttgtgatac cattttccat cttttgcggc aaccagcctg aatgtgttgc
     4561 cgccttccga aagtatgtga tgggtgacct tgctgatatt ctccatcatg gcaggaatgt
     4621 tcgggaagac tgagcggttt ttgcattgca ggatgtattg ctgccttgct gaggttttga
     4681 ccagaaaggt cttgtttatg aaaccttccc ccagagctgc cacacacata acatgctccg
     4741 ggatgcaaaa gtgcgtgaca atcagctgtg catcgcccat agtggatcac aaaactaaag
     4801 gaacgcaaag atactaaata aatagttcag gggcaatctt ttttatcatt cccatgagca
     4861 ccgttcccgg tccgtattcc gtgaaattcc gggcgccatc ggccaccata tgctgaatgg
     4921 tctgggccca tctaaccggc gatgtcaatt gcttcaccag gttttctttg atctctcccg
     4981 gatctgtatg aggaagtccg tcaacgtttt ggtaaacagg acatacaggc ttgaccagga
     5041 cagctgtgcc tatcgcatcc tccagcgatt tgcgggcact ttccatcaaa ggagagtgga
     5101 atgctcctcc aacaggcaat acgacagccc tttttgcacc ggcttccttc atggccacac
     5161 aggctctttc caccgcttcc acggtccctg aaatgactat ctggcccgga caattgtaat
     5221 tggccgggac cactatgcaa ccggaggccg aaatcgtagc acagatttct tcaactttct
     5281 catcttccag ggtcaataca gcagccatgg tggaaggttg ctcttcgcag gctgcctgca
     5341 tggccagggc cctggcactt acaagacgca agccctgctc aaaggacaag gccccggctg
     5401 cggtcaaggc agaaaactcc cccagggaat gcccggcaac catatcgggt ttgaaatcgg
     5461 ggtcacacaa tgctgcggcg acggaatgca ggaaaatggc cggctgtgta actttcgtct
     5521 gtttcaattc atcggctcct ccttcaaaca tgatttttga aagagaaaag cctaaaatgc
     5581 tgtctgcccg gtcaaaaagc gctctggctt ttggattgga atcgtacaaa tccttaccca
     5641 taccggtaaa ttgggcaccc tggcccggaa atacatatgc tctcataagt caaatataca
     5701 ttttttttca atgtccggga atctgcgtat ctttacatcc cttcaataag ccggcccagt
     5761 agttcagttg aatagaacgt cagattccgg ttctgaaagt cgggggtttg agtccctcct
     5821 gggtcacgct acaagaaaaa aaaagctgtg gggagcgaac acgcttcctg cagctttttt
     5881 tgcttgggct gtattcggcc caggagggaa cgccgagcgg agcgaaggca atcccttttt
     5941 tgcgcagggc aaacccggtt taccgcgcaa taccaatttg ctaaaatata aatatcagat
     6001 tatcaacggt ctgcaaaaca cgaaatccct cttgcgcggc actccgggtt tgccaccctg
     6061 tcacagtgtg gtcctgaacc attgggaggt gcggcccagc atcctcatgc cggccacgaa
     6121 gccacggagt ttcatcaggt cgttgctttc caggcgggca aagcagtcat aagtcttgcc
     6181 cgaggccgga tcgtatatgg ttccgtcttt ccactcgttc tttttttcat tgtatacaaa
     6241 accattcagg atccgaagac ctatgagtga cctgctctgg agggccggat cgggattttt
     6301 gtcgtctttg ggttcttccc cgttttcata cttttcagga atcatatata tgattgtacc
     6361 gatatatttt ccatccttca gttccacctg aatcttggtg gttttatctt cattccacca
     6421 tacaccggtg attttgcctg catcctgtgc aagggctgta agcgaataaa cactgagaaa
     6481 caacaaaacg gcaagttttt tcataacatt caaattagag tttatttgac aaatatagaa
     6541 aaaagtcgaa atcgtgtact tattcctgcc atcagatagg ctgcaacagc cacccacata
     6601 accgtctgat aacctgcctc tactgaaatc acagccgcaa gggccgtact gattacagag
     6661 aaggatccat tgacggccca tgcccatgga ataagggatc cgtcacccac atgtcttatg
     6721 gccaggattg ccgaggggaa catcatgccc atcaggaacg ccggaggtcc aatcaccagg
     6781 gcacacagca atacctttac cggaagcgga tttccgatgg tcgctttcat cagggaaggc
     6841 aggaacaggg ccaaaagtgc cagcagcacg gcaatcactc ctgccacgag ccgtaagcgg
     6901 gggctgccgg tgaccatttt ttgggaacag gccgagccgg tcccggaaaa aaagagcatt
     6961 gcgctcagaa cggccgaggc agcgtagagc ggactgccaa aaaacaaggt aaattgctgg
     7021 atgaaaacca tctcggcaaa catataacca atgccgatga acgagaaaaa gaccagcagg
     7081 cttgttttcc ccttcccagg cacttttttg aaaatcagcg gaagcacaat aaacaggaaa
     7141 gaggccagga aaatctgcgc catggtcagc agcacaatca ggtaaccgac ttcgaggaaa
     7201 ggcatagatg ccgatccaaa tacctcccgc attttggaca accgccccaa tttgagaaac
     7261 tgagaaaaat agggtcggga atcggtggcc ggccttatct gaaaatcata ttccctgaac
     7321 agtttttccc tttcccgtgt cagcagaaga tcggttgtcc ggaaaaagga cttgtcctgc
     7381 aacatgttgt acacttctct ttcttcagga tggatccccg gatagagcag gggatcgaac
     7441 tgccgagact ggcagaatgt gcggatctta tcaatgttgt tggccgatac cttgtttttt
     7501 accaggacac aggtgatggt tgaccagctt cgcactgcca caatatggtc tgccgggtct
     7561 tccacccctt tggattctgc tgttactgcc atggtagcca gcaacttaaa ggtgctcctt
     7621 ggtggatagt ccacccacag ggttgcagca atcataccgc cgggggacaa cctgtcccaa
     7681 aggagcgaaa aagcatccag ggtgaacagg tattgctctt ccagggccag gatgcccgaa
     7741 ctgcctccaa aggaaccagt ttcgggcagg aggatgagat cataccggtc gtcctgctgc
     7801 acatgatacc tggaatgccc cgctatgaaa cttatttgtt ccccgggcac ggcaatgttc
     7861 ctggtgacca tccctaccac tgtgctgttg ggttccacag ccacgatctc tttggctccc
     7921 cggctaaggg catatccggc ctgcagtccg gtggcggcat cggcaacgaa taccttcccc
     7981 ggggtgccaa taacataggg gagtgcctgg gttgaataat ccagatagtg cccggtttcc
     8041 ttgccatcaa gggattcctg tgcgccctcc gcgtccggtg tgcccggcag ggctgacaag
     8101 accaccgttt cttcccgttc cggcagtgcc atgggcacaa gcgggcccag ccagtccccg
     8161 ttatggaaga gcacttcggt taccggtacc tgtccggtat aaccaaggct cagcccaggc
     8221 gcataccgca gggcaggggc agacacgcgt ttgagttgtc cgtagggact gtttatggtt
     8281 tccaccaccc tggcatcggg cagcaacagg gctcgtgaca ggcttttata ttgtgaggag
     8341 ggaaggatgg gaggattcag gaaacctgcc acaagcagga caagggccag cagcgccagt
     8401 gtaaggaggc ccgttttttc agcaactgtt ttcccgttgt gccggaatat cagcaaaccg
     8461 gccaccagag gaagacaggc gatcacaaaa ggtactacag acggggatac atgccatagt
     8521 agtatcaaca tcagcagtcc ccccattccc gaacctgcca gatcagcaaa atataaacga
     8581 ccaatatctg aggcataatg actgtaagcc agaccaagag cagatgctcc caggaaaaag
     8641 gggacaaaca acatcaggta ggaacagatc aggttcaggt agtggcgttt ctcgtaaaaa
     8701 accaggtagg aatcaaagcc tccgaacact gtgcgggaga aaaaaagaat tcccatgatg
     8761 cttaatgaag tgaatatcat gagaaaagcc aggttggacc cgatttttgg gaccaggtga
     8821 tttctcagga aagccagggc ggtcccgctt gctccgaaac caagcatggc caccgatatt
     8881 accatgtagg caaaatgatt ccactgagag atggacaata tctgcatgag ggcaacctga
     8941 aatgccacca tactgcagga tagcaggcct atggaaattg ccagtcttct tttcatgatc
     9001 tttctttacc gggtttcttc ccttgtaaaa gggacaaaac ggaccggcat cagattggtg
     9061 gtagtgatgc gtcctttctt cttttccacc aggatcaggt tttgtaccat gaactgggat
     9121 ccaacgggta tgaccatttt acccccttcc ccaagctgtt ctatcagagg aggcggtatg
     9181 tgttcggcag cggctgtcac cactatggcc tggaagggcg catttccggg caacccgtaa
     9241 tacccgtcgc ccggtaccac ttcaacgtta tggtatccca ggtcctgcaa cctttttttt
     9301 gcactggccg caagttcttt cacaatttct atggtgtaga cagaatctgc catccgggcc
     9361 agtatggccg cctggtaacc cgatcctgtg cctatttcaa ggaccctgtc ttccggcttc
     9421 aggtccagca ggcttgtcat gtaggccaca atgaagggtt gggaaatggt ttgcccgtat
     9481 cctatgggaa gggggttgtc ttcataagcc cttgagctga gggaagcgtc tacaaaaaga
     9541 tggcgttcta ccgtgcgcat ggcctgcagg gttgcgcggt gcgttacccc ccgtgcaacg
     9601 atctggtccc ggaccatggc actgcgcaag cgtgcaaaat cctgctcccc ggtgcctgtc
     9661 agtatggcag acagggctgg cagggctgct gtaacaagca ggacggtcaa tgggtatttc
     9721 aggatcctgg ccatggatcc gtaaagttat aaaaatttca tcaaggtgtt aaaaacccgg
     9781 gatttgacct catccggccg gggattcagc tcttctctgg taaactgtcc attttccgga
     9841 tcaatgcgtt ggaaaaccat aaggtacaag tcaaagatga tctgaagact taaccggtaa
     9901 cgcgtatcca gcacgggaga gacttttctg atcatgttaa gggtttccct gcggtatgca
     9961 tcggcaacat ccatgtagtg cctgaccagg agacggaatg aaggcagcac ggtgcctccg
    10021 cgggccattg tgtccagctg ctcctggctc aacccgtatt gaagaagaag gtcggaaggg
    10081 aaataattca ggttatccag cctgtcttgt tgaaaatccc tgatgatatg gaccaggtaa
    10141 ctgaagatgg cgcagggacg tgccgtctca cgggcatcga aaacgggggg cagataatga
    10201 tcgttttgct tccggaggcc gcacaggtgc acaaaaattc cggctggtgc cacagaggcc
    10261 ccctcggcat attcaagaaa agaggaaaaa gtggggaaac cctggtggtg aatgtcataa
    10321 atcatggatc gggtaaaagc ctctacgggc cagaggggaa tcagaaattt ctcccaggta
    10381 gctgtcagct cgctgtccgc agccatgttg gagttgttca tgagcaatcc ggtcagtctg
    10441 tccagattat tcctgagagc agcctgttcc tgttctccga taacagggtg ttcagattta
    10501 taattgtcta caagatcatc tgccgatcgc aaaaaacagt aggccgtgca ggctgcccgg
    10561 tagcgttccg gctcccagaa agcagcagct atcaggatgt ttggatgctt gtcaatatgt
    10621 tcaaaatcca gggattgaaa tatctccagt atctggtcct tgtcagatct tctactcatg
    10681 tccgtgttcc ttaataatcc gttccgaggt caaagctgca gacatcagta ccagcggcac
    10741 tccgtttccg ggatgggtgc taccacctgc aaaatacagg ttcctgtagg tgcggtgccg
    10801 gttgtgcgga cgaaaatacc ccatctgcaa aacgaagtgg ctgatgctgc caaacacaga
    10861 tcctttggtg acgtggaatt tagtttccca tgtttcgggc atcaggcatt tttcaaattt
    10921 aatatgctgc tcaaaatccg taagtcccag tttttcaaat ctttccagca cccctttgcg
    10981 gacctgttcc cggatggcat cccactggtc tttgtttttc ttgtgcaaat ggcctacagg
    11041 cactactacg gagatggtgt cctgtccttg tggagcggct ccgggatcgg tttcccgggg
    11101 acaaaacaca taaaaactgg gatcatccga tacttttttt tctttgaata tgtggtccag
    11161 gttgtttttg tagttttcag acatgaaaac attgtgcagg gtgagttcag ggtatgtttt
    11221 atccattgcc cagtgaaatg atattgccga acaggaatac cgcagcttgt ccagccaggc
    11281 tgtataacac ctgtcctcaa gcaactccct gtacacatag ggcagatctg cgttggcaac
    11341 caccacatcg gccggaacag ccgttccgtc ttccagttca atacccgtgg cctttttccc
    11401 actggtggtg attttcctca ccggactatt gtaatgaaaa gtcacgccga gttcccgggc
    11461 agtcttcacc aggctttcca caacactgaa cataccgcct tcagctacca gcgatccctg
    11521 gctgatctcg gcggcgggga gcatggaaaa aagcgccgga gcggtaaacg gattttgacc
    11581 cacataaatg ttttgaaagg tgaaagcggt ccgcaggtgg gggtgattga aaaagcgctt
    11641 aatataacgc atatgactgg tcagggtctt gatacggaac aggatgctaa tgtttttcca
    11701 tgttatgaac tgaaacaggg aagtgtaatt gcgccccagc agctggtcaa tggcaattcg
    11761 gtaaaaaccg tacccttttt ccacatactc ctggaaacgg gcataacttc ccttttcgag
    11821 ggcctctagc tgcttttcca tgacctgcct gtctgtggta aaacggaagg tggttccgtc
    11881 tgcgaaatat agacggtaaa tctcgtcaag gtgttttgat tttatggcac tgtcaaaaga
    11941 aatcccaagg gagtcaaaaa ctttcctgta aaggtcaggc atcagaaaga tggtggcccc
    12001 caggtcaaaa cggtgtcctt cccggatata gcagctgcac cggcctccgg gggtgtctga
    12061 ctgttcgtat acgtttatgc ggtatccttt tcttgccaga aatatggcag tagctatgcc
    12121 acctgttccg gcccctatga tgacaacatt attattatcg ttcattgatg gaaaaaatat
    12181 aacaaagtcc aaatataatt tgaatatttg tatgcaaaaa atacgtttaa ctatatgaaa
    12241 aggattgttt tgttgtttct gtgttttgta tgcctgaatg gctgcgaccc ggactcatgg
    12301 cgaccttcat gcgctcctca tgcgtatttt catttttcat atgaaaacca tgcctgggtt
    12361 tctgtacatt ccggctggat aattgacgaa aacgggatca tccggtccta taagaatcca
    12421 cataaatgga attatcccga ttcgttggga tatattacca ggtttgaatt ggaggaaaac
    12481 ctgtcttact gcgactcggt aatcgggaaa gtttctgcaa gagaaatgtc atactacaat
    12541 catttggtat tcccggcctc taagggttca ctgaccaagc ccgaaccaca tggcgccgac
    12601 atgggcatcc ggatgtattc ctgctattgg tatgaccaac ggaagggccg gtaccggcag
    12661 gtgatattga atcagtatgg agattggatc cgaaccaacg aaaatgacaa ggctgtgaaa
    12721 atttattcat ggctcagtag gctggttaca aactgatcat ctgccaattt tatgtatctt
    12781 tgctcatgta tgcggttgac tattacacat tacggagcat ggatcgcctt gctggcgttc
    12841 gtatcgtgtg ggacaccccg tctggggact tattccatcc gtttgcagga tgcagaaagc
    12901 atccctgttg ccccgacccg tgacatgatg ctttcaactg ccgaacaacc cgatgggacc
    12961 acgctttatg tttatgaaaa tcccatgttc aggattacat ggtccctgac aaacacccgg
    13021 ttcgggttta tcctggacaa ccttactgca gacactttgt tcattgactg ggataaggca
    13081 tcctacctgg accaggacgg agacacactc aggttgatcc atgacggggt tgattttttc
    13141 ctgaaagact ctatacagga gataacaccc ctggagcccc tgggatcgct ggaagatttc
    13201 ttgctgcctg cttccaacat acattacgat cctgacaatt ataccctatg gaggatcaac
    13261 cacctgtttt acgacagaaa aggaaatatc ggacagcgtg tatcggtgga acttcctctg
    13321 gagattaacc gtatcatata caggtatcgg ttcaggttca caatagatga ctggaaagca
    13381 gactcttttt aattgtggat tccatttttt gttttacttt tgcagtccct gaaagtaatg
    13441 gtgatcgtag ctcagttggt tagagcgcta gattgtggct ctagaggtcg ggggttcgaa
    13501 tcccctcttt caccctgaat aatgagaagt tttgttcatt ttttttggag tttcaaattt
    13561 ctctttatct ttgcaacccc ttttgcgagg gagaataaaa aagttcattg acagattgga
//
LOCUS       DAOSOF010000045        13539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4574_length_13539_cov_5.670202, whole genome shotgun sequence.
ACCESSION   DAOSOF010000045 DAOSOF010000000
VERSION     DAOSOF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4574_length_13539_cov_5.670202"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLM68_03945"
     CDS             complement(<1..349)
                     /locus_tag="PLM68_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT54549.1"
                     /translation="MKNYDILELFRYQAKHTAIYARYLDLLEINPQKVNSPEEIPFLP
                     IRFFKTHRVYNASCPPEHVFTSSATTGTVPSRHYVDSLDRYGKNVLDIFRFFYGDPSR
                     YTILALLPSYLERP"
     gene            complement(336..1868)
                     /locus_tag="PLM68_03950"
     CDS             complement(336..1868)
                     /locus_tag="PLM68_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOT54550.1"
                     /translation="MSLINQAYRLYNRRLLTELDFSSQDPARFQDATFQYLIRHGANA
                     LFGKTYKLGEIQDVKAFQERIPLHDYDMLEPYIQQSLKGIENVLWDMPVYWFALSSGT
                     SSSRSKFIPMNRDNLNLCHYKGMLIMLSHYVRTFPKSRLFTGKSLTLGGNRHIDKNVD
                     SNCCYGDLSAFLIINTPLLARLVTTPGKKIGLIPDFEAKIEQVSRLIARQKLTSFSGV
                     PSWNLILMRKVLAETGKKNLHELWPHMELFMHGGVCFEPYRVHYRELFPSPDMHYMET
                     YNASEGFFAFQTDLNDPGLQLLPNVSVFYEFIPLDKLAAAQNGSFTRFLTLSEVRTDQ
                     PYAMVISTNSGLWRYLIGDTVRFTQLYPHKIIISGRTKLFINAFGEELMIENAERALA
                     RACTMTGASVHEYTVAPVYMDGQNKGAHQWLIEFEKMPPDPEAFCGMLDKALMEVNSD
                     YQAKRLDTGTMDPPLITSLKQGSFYRWLAFNNKLGGQTKVPRLWNTREYAEALLEVNE
                     KL"
     gene            complement(1883..2611)
                     /gene="recO"
                     /locus_tag="PLM68_03955"
     CDS             complement(1883..2611)
                     /gene="recO"
                     /locus_tag="PLM68_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT54551.1"
                     /translation="MITPLTLIILNTRPYRETSMLVNAYTDTRGRADFLVKGIRTARN
                     RALASLFHPLSILDTRTYFHAKRELNQLKEYQKVYLLEDLCSDARKSSIGLFISELLY
                     KTVRESEENRELFRFVTDTILGLDRAGDELADLHLYFAVRLCRYLGYEPDAESFLNSR
                     IYFHMGKACFCETPDPMSGAFDQEDSLLLHQILSCPAGQIRPLSCSGSRRHHFLHSMI
                     SYYGYHLGKAPEIRSLDVLHQVFL"
     gene            complement(2608..3177)
                     /gene="rdgB"
                     /locus_tag="PLM68_03960"
     CDS             complement(2608..3177)
                     /gene="rdgB"
                     /locus_tag="PLM68_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT54552.1"
                     /translation="MELLFATANLHKRQEAATILGSSFSIIVPSSLGFSGEIPETGLT
                     LEENALQKARFIWDKFRIPCFADDTGLEVEALNGAPGVYSARYAGPSCSYTDNVNKLL
                     EEMKDITLRKARFRCIIALILDGRPVLYEGIVPGSIITAPRGNNGFGYDPVFVPLGYE
                     KTFAQMTEEEKNTLSHRKRALGKMALALQ"
     gene            3230..4783
                     /locus_tag="PLM68_03965"
     CDS             3230..4783
                     /locus_tag="PLM68_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT54553.1"
                     /translation="MRIKSILAALFLPLLLHGQTAEFNTSRSLDIFHSLLREISLYYV
                     DSVAVDQLVYTGIEAMLSSMDPYTEFIPEEENESIEMMTTGSYGGVGALIKKRPGDGV
                     LISEPYAGSPAARAGLIAGDVILAINGESVFDLTADECSTRMKGTPGTGVDFLVQRLM
                     GGDTVSVTVIREKVHISDVGLACMLQDSIAYIRMTGFTQGGAADVRKALKELKKGHPV
                     RGIIVDLRGNGGGLLDEAVKLLSIFLPKNTMVVSARGRIQRFDVEYYTKEEPLDTETP
                     LVILVNNGSASSSEIVAGAIQDLKRGIIVGEKTYGKGLVQTIRPLSYNANLKITTAKY
                     YIPSGRCVQSLDYRKRNGDGTPERNENGGIMPDSLVPLSMYSRIAMELVARDYLHDYS
                     IMYYTRNETIPPAEAFYLSDEDYLDFVKITAAKEFDDRSATEILLETFSRTAKAEGYE
                     ELLEKEIVALKERLLTDKARDLIHYRKEIQPLLEEEISCRYYMQEGRLRSMIREDSQL
                     DKAISVFIR"
     gene            4825..5262
                     /locus_tag="PLM68_03970"
     CDS             4825..5262
                     /locus_tag="PLM68_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT54554.1"
                     /translation="MPLEKKIDSSPMNNVTRISGDTKIIGNITTQHDIRIDGYLEGKI
                     KTDGKLVIGETGICVGEAECESVDISGAFDGVLKVSELTSLREKCVFTGELHSDQLSV
                     EPSVRISGQLISPELSKSKDKKSVPPTVSAPREPAPAVAPETK"
     gene            complement(5268..6578)
                     /locus_tag="PLM68_03975"
     CDS             complement(5268..6578)
                     /locus_tag="PLM68_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT54555.1"
                     /translation="MKTRSLNRQILNLAIPSMLAGITIPLVGMADTAIAGHLGSATAI
                     GGIAIGSTLFDLLYWNFGFLRIGTAGITAQAFGKGDRQEIIRTGMQGMILALAFSLLL
                     ILIQWPFAHLVILLSPSSPEVEALSLEYFFIRIWAAPAVLALFVFRGWFIAVQNTLAS
                     MIIDVVINVTNIGLSILFALNMGMGIKGIALGTVVAQYTGLLCGVVLLFVLYRKMIRG
                     VPLVAAIKTGGWKRYFNLSGNLFIRSICMLLIYTGFTFLAAQYGDTLLAVATIMMKLL
                     LLFSYFVDGFAYAGEALTGKFIGAGDKPSLMRSVKLLFLWSTLVGVISTVFYVIGDEW
                     LLRLMTSSPDVIRAARPFLPWLYSMPLISCLTFMWDGIYIGATAGKAIRNVMIISVIA
                     FYAFYLALENPIGIQSLWAAYAAHLVARSAAQTIMARKHVFNLI"
     gene            complement(6585..7553)
                     /gene="rsgA"
                     /locus_tag="PLM68_03980"
     CDS             complement(6585..7553)
                     /gene="rsgA"
                     /locus_tag="PLM68_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT54556.1"
                     /translation="MKQRLFENATVVKQTGSLYRLTFLPRWEPFSAILSGRLRLREDK
                     LTNPIAVGDRVNGFWQPDGYNKKEQAVITEIHPRVNYLIRKSVNLSRQAHIIAANLDR
                     ACIILSATEPQTPFPFIDRFLVTCQAYGVPATIILNKTDELEWAPEYRSQADQYKDVY
                     QRAGYTVLEVSAKTGEGTDMLAQDLKGKISLFSGISGVGKSALANRLDPELRLRTAPI
                     SLAHMTGKHTTTFYEMHPLRSGGFIIDSPGIKGFGLLEMTREELYHYFPEIFAAAASC
                     RFNGCLHAEEPGCAVKAAVEKGLIPAQRYDSYLRMLEDPEGGKYRT"
     gene            7613..8584
                     /locus_tag="PLM68_03985"
     CDS             7613..8584
                     /locus_tag="PLM68_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT54557.1"
                     /translation="MFTVNEIQGFVNQGLQNLIKSYEHSRLHGPVEYALSGGGKKMRP
                     VLCLLSYNIFSDKLPASILYPSLGLEVYHNFTLLHDDVMDNSDKRRNQPTVHKKWDVN
                     TAILAGDAMCMLAYSYISKCEPKVLPPVMDAFHKAAEQVCEGQQLDMDFEKQLFITED
                     EYFDMVSKKTGALIACSLQLGGLCAEASSRSVNNLFNAGMALGRAFQIQDDYLDVYGD
                     PQVFGKSTGTDIANNKKTWLLTYALRNATGNNLAELNRLLTHKEDPGRVEQIIQIYNK
                     LSVGKTACERIAHYLDTALNALGSLDVREGRNTVLIDFIKGLIDRKR"
     gene            8586..9197
                     /locus_tag="PLM68_03990"
     CDS             8586..9197
                     /locus_tag="PLM68_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOT54558.1"
                     /translation="MNIASFSEHCNGLFDAAIRDYHRQNDTDAPYSNPYTEHSLDYLL
                     YRKNLIDTVQWHLEDIIRDPAIEPAEALRIKRRIDASNQERTDVVEQLDLMFNREFSG
                     VPRLPDASLNTETPAWAVDRLSILALKIYHMNIEAERKDAPAASLCRQKLEILMIQRT
                     DLSGAIDQLLADLKTGRKYMKLYKQMKMYNDPLLNPILYEKEK"
     gene            9181..10170
                     /locus_tag="PLM68_03995"
     CDS             9181..10170
                     /locus_tag="PLM68_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOT54559.1"
                     /translation="MKKKNRIPHVLCLRFSALGDVAIASIVLKACARDNPRVFFTLAG
                     PGLLAPLFAGVPNLFLLSVDKKQPLRIIFRSLRKVHPTHVADLHSVIRSFILRSAFFL
                     SGTPVVRLHKGRRSRRRLLKNPGHAPALVPMYQLYQQTLEKSGFKSPSLIRDNIIPLE
                     RGPWKRIGIAPFALQKGKQWPAERMHQVADRLARENTLVFLFGGGDEEMAHMKAWAAG
                     NSNIRLAADAGSDFEAELAVIKTLDVMISMDSANMHFASAMGIPVISIWGATHPKAGF
                     YGWRQDPDRAIQLPMDCRPCSIFGAKPCWKGTYACMEDLTAEQVYSYVRSVME"
     gene            10148..11401
                     /locus_tag="PLM68_04000"
     CDS             10148..11401
                     /locus_tag="PLM68_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOT54560.1"
                     /translation="MSGLSWNKPEIMAPAGNWASLTAAAQGGAHAVYFGVGELNMRAH
                     AAHNFRREDLPEITAFCRRNGMRSYLTVNTIFYDQDLEALHAILQAAADAGVSAVIAS
                     DMAAIEEARSLGLEVHISTQLNISNIRALAFYARFADVVVLARELTLDQISAIAREVK
                     ERNITGPSGNLVKLELFCHGALCMAVSGKCYMSLHQYNKSANRGACYQVCRRSYTVTD
                     KETGEAMEVDNQYIMSPKDLCTVRFLDKILDSGIEILKIEGRARSPEYVKTTAQVYSK
                     AAEAVVNGTFTPQLAAGLEEQLKTVFNRGFWGGYYLGESLGEWSETYGNQATQKKTYV
                     ARITNYFARQGVAQLKVEAVPLHAGDEIMIIGPTTGVVEERVREIRLEGGSAVSMAPK
                     GTVCSIPVTVRLRRSDKVYRLEKND"
     gene            complement(11398..12294)
                     /locus_tag="PLM68_04005"
     CDS             complement(11398..12294)
                     /locus_tag="PLM68_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOT54561.1"
                     /translation="MNKKLLMIRPCCFGFNPQTAVNNAFQVATGEDSGLVQEKALAEF
                     DSYVTLLQQHDIDVIVVQDTPEPHTPDSIFPNNWISFHPGQMSVLYPMYAPNRRLERK
                     KTVFRAIQEHAPRIKWIDMSPRERENRYLEGTGSMVFDRDNNRAYACRSPRTDEGLFR
                     DICDTLGYQAICFDALEKGSAIYHTNVMMCMARDYVVICLESIPSEADRQMLLDVFIE
                     DRKTVVEITAKQMNHFAGNMFQVFSRKDKPFLVMSKRAYKVLTPSQRKILRSFNKLIT
                     PELDTIETHGGGSARCMIAEIV"
     gene            complement(12287..13504)
                     /gene="rocD"
                     /locus_tag="PLM68_04010"
     CDS             complement(12287..13504)
                     /gene="rocD"
                     /locus_tag="PLM68_04010"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOT54562.1"
                     /translation="MKSQDYITLEEEYGAHNYHPLPVVLSKGKGPYVWDIEGKKYFDF
                     LSAYSAVNQGHCHPKIVKALTDQAKQLCLTSRAFYNDSLGPYEEYITRFFGYDKVLTM
                     NTGAEAVETALKLARRWGYDKKNIPANEAKIICCEGNFHGRTITVISLSTDSSAYEGF
                     GPFTPGFIKIPYNNPNALEKVLREYGPHVAGFLVEPIQGEAGVMVPDEGYIKKCYDLC
                     KEHNVLFVADEVQTGIARTGKRICCDHEGVHPDILILGKAISGGVLPVSAVLSNDEIM
                     LTIKPGEHGSTFGGFPLACRVAHAALNVVEDEKLAEKATYLGEIARNFLRSIPSDRIE
                     LVRGKGLLNAIVITPKNGVEAWDICMAMAKNGVLAKPTHRHIIRLAPPLVIKESQLMQ
                     ALEIIRKTILSFE"
BASE COUNT         3485 a         3293 c         3499 g         3262 t
ORIGIN      
        1 ccggtctttc caggtaagac ggcaacaggg ccaggatggt gtaccgggag ggatctccgt
       61 aaaaaaaacg gaaaatatcc aggacgttct ttccataacg gtccagactg tctacgtaat
      121 gccgtgacgg gaccgtcccc gttgtggccg agctggtaaa cacatgctcc ggagggcagg
      181 atgcattgta taccctgtgt gtcttaaaaa agcggatggg aaggaaaggg atttcctccg
      241 gggaattcac cttttgcggg ttaatttcca gcaaatccag gtaccgggca taaatagcag
      301 tatgttttgc ctggtaccgg aacagttcca gaatatcata atttttcatt aacctcgagc
      361 agggcttcag catattcccg ggtgttccac aaacggggga ccttggtttg tcctcccagt
      421 ttattgttaa aagccagcca acggtaaaag cttccttgtt tcagactggt tatcagcgga
      481 ggatccatgg ttcccgtatc caggcgtttg gcctggtaat ctgagttgac ttccattagg
      541 gccttgtcca gcataccgca aaaagcttcc ggatcaggag gcattttttc aaattctata
      601 agccactgat gggcaccttt gttctgcccg tccatgtata cgggagcgac cgtgtattcg
      661 tgcacacttg ccccggtcat ggtacaggcc ctggcaagtg ccctttcggc attctctatc
      721 atgagctctt ctccgaaggc gttgataaac agctttgtac gacctgaaat gatgatcttg
      781 tgtggataca gctgtgtaaa acgtacggtg tccccgatca gataccgcca caatccggaa
      841 ttcgtagaaa tgaccatagc gtagggttgg tccgtacgga cttccgaaag ggtaaggaac
      901 cgggtgaatg aaccattttg agcagccgcc agtttgtcca ggggaatgaa ttcatagaat
      961 acactgacat taggcagcaa ctgcagtccg ggatcgttca ggtctgtctg gaatgcgaaa
     1021 aatccttccg aagcattgta cgtttccata taatgcatat ccggtgacgg gaacaactcc
     1081 cggtaatgga cacgatacgg ttcaaaacaa acaccgccat gcatgaaaag ttccatgtgg
     1141 ggccacaact cgtgcaggtt ctttttgccg gtctctgcca ggaccttgcg catcaggatc
     1201 aggttccagg aagggacacc ggagaaactg gtcagttttt gacgagctat caggcggctt
     1261 acctgctcga tttttgcctc aaaatcggga atgagcccta tttttttgcc aggtgtcgtg
     1321 acaagtctgg ccagcaaagg ggtgttgatg atcaggaaag ccgacaaatc accgtaacag
     1381 caattggaat ccacattttt gtcaatatga cggtttcccc caagggtcag ggattttccc
     1441 gtgaaaagcc gggatttggg gaatgtcctt acataatggg aaagcatgat gagcatcccc
     1501 ttgtaatggc ataggttcag gttatccctg ttcatgggaa taaacttgga cctggaagaa
     1561 gaagtgccgc tggacagggc aaaccagtat accggcatgt cccatagtac attttcaatg
     1621 cccttgaggc tttgctgtat gtaaggttcc agcatgtcat agtcatgcag gggtattctt
     1681 tcctggaaag ccttcacatc ctgtatttcg cccagcttat atgttttccc gaacagggca
     1741 ttggccccgt gacgtatcag atattggaaa gtcgcgtcct gaaaccgggc agggtcctgt
     1801 gaagaaaagt ccagttccgt gagcaacctt ctgttgtata gcctgtaagc ctgatttatc
     1861 aacgacatag attgcaaagt tactaaagaa atacctgatg caacacgtcc agggaacgta
     1921 tctcaggggc tttgcccaga tggtaaccgt aataggatat catgctgtgc aggaaatggt
     1981 ggcgtcttga acccgaacag gataaaggcc tgatctggcc cgcggggcaa gacaggatct
     2041 gatgcagcag cagggagtct tcttggtcaa aagccccgga catgggatct ggtgtttcac
     2101 agaaacaggc cttacccata tgaaagtaaa tcctgctgtt taaaaaactt tccgcatcgg
     2161 gctcatatcc caggtagcgg caaagacgca ctgcaaaata aaggtgcagg tcggccagtt
     2221 catccccggc cctgtcaagg cccaatatgg tatcggtgac aaaacggaac aactcccggt
     2281 tctcttcaga ttcccttact gttttatata acaattcgga tatgaacaat ccaatgctgc
     2341 ttttacgtgc atctgagcac aaatcctcca gcaggtaaac cttctggtat tctttaagct
     2401 gattcagttc acgtttggcg tgaaaataag tgcgtgtatc aagaatgctc aggggatgaa
     2461 acagggaagc cagggcccgg ttgcgggctg tccggattcc cttgaccaga aaatcggccc
     2521 ttccccgtgt atcggtatag gcattgacaa gcatgctggt ctccctgtac ggcctggtgt
     2581 tcaggataat tagcgtaagc ggggtgatca ttgcaatgcg agagccattt ttcccagggc
     2641 tctttttctg tgggacaggg tgtttttttc ctcttccgtc atctgggcaa aagttttttc
     2701 ataacccagg ggcacgaaaa ccggatcgta gccaaagccg ttgttccccc tgggagcggt
     2761 aattatggat ccgggtacga ttccctcata caggacaggc cgtccgtcaa ggatcaaagc
     2821 tatgatacag cggaaacgtg ccttcctcaa agttatgtct ttcatttctt ccagcaattt
     2881 attcacatta tccgtgtaag aacaggaagg tcctgcatag cgggcagaat aaacaccggg
     2941 cgccccgttg agcgcctcca cttcaagacc cgtatcgtct gcaaaacagg ggatccggaa
     3001 cttatcccag ataaaacggg ctttttgcaa cgcgttttct tcaagggtaa ggcctgtttc
     3061 cggtatttca cctgaaaagc ccagggatga cggcacaatt attgaaaatg aagagccgag
     3121 tatggtggca gcctcctggc gcttgtggag gttggccgta gcaaataata attccatatt
     3181 caaagatatg aagaattggt gtaaatttgt ctggtaaaca cttatcgaaa tgagaataaa
     3241 atcaatactt gccgccctgt tcctgcccct gttgctccat ggccaaactg cagaattcaa
     3301 cacttccagg agcctggata tctttcattc cctgttgcgt gagatatcac tctattatgt
     3361 agactctgtt gctgtcgacc aattggtgta cacgggaata gaagcgatgc tctcttccat
     3421 ggacccctat acggagttta ttcccgagga agaaaacgaa tcgattgaaa tgatgactac
     3481 ggggagctac ggaggggtgg gagcccttat aaagaaacgt cccggagacg gagtgcttat
     3541 ttctgaaccc tatgccggct caccggcagc cagggccggg ttgatagccg gagacgttat
     3601 cctggcaatc aacggggaat cagtgtttga tctgacagcg gatgaatgca gcacaaggat
     3661 gaagggaaca cccgggaccg gtgtggattt tctggtgcaa aggcttatgg ggggtgatac
     3721 ggtttccgtt acagtgatac gagaaaaagt acacatatcg gatgtggggc tggcctgcat
     3781 gcttcaggac agcattgcct atatcaggat gacaggtttc acccagggag gtgctgccga
     3841 tgtgcggaaa gccctgaaag aattgaaaaa gggccatcct gtcaggggca ttattgtgga
     3901 tttgcggggc aacggagggg gtttactgga cgaagccgta aaacttcttt ccatcttcct
     3961 gcccaagaac accatggtgg tttccgccag gggccgcatc cagcgctttg atgttgagta
     4021 ttatacaaag gaagaacctt tggatacgga aactcctttg gtcatactgg taaacaacgg
     4081 gtcggcgtct tcctcggaga tcgtggcagg agctatccag gatcttaaaa gggggatcat
     4141 tgtgggagag aagacttacg gcaagggact ggtacagacg atacggcccc ttagctacaa
     4201 cgccaacctg aaaatcacca cagctaaata ttacattccc agcggaaggt gcgtacagtc
     4261 tctggattac aggaaacgaa acggtgacgg gacaccggaa cggaacgaaa acggaggcat
     4321 catgcccgac tctctggttc cactgtccat gtatagcagg attgccatgg aacttgtggc
     4381 cagggattac ctgcacgatt attccatcat gtattacacc cggaatgaaa ccataccccc
     4441 tgcagaggca ttttacctct ctgacgagga ttaccttgat tttgttaaaa ttaccgcggc
     4501 aaaagagttc gatgaccgca gcgccaccga gatcctgctg gaaactttct cccgcacagc
     4561 aaaagccgag gggtatgaag agttgctgga aaaagaaatt gtggcattaa aggaacggtt
     4621 gctgacagac aaagcccgag acctgatcca ttaccgtaag gagatacagc cgttgcttga
     4681 agaagagata agctgccgtt actacatgca ggagggtcgc ctgagaagca tgatacgtga
     4741 agacagccag ttggataaag ctatatctgt gtttatccga taaacaaaaa aatgttaatt
     4801 ttgggaaata ataaaatcaa ggccatgcca ttagaaaaaa aaatcgattc atcccccatg
     4861 aacaatgtga cacgtatatc cggtgacacg aagattatcg ggaatattac cacacagcac
     4921 gacatacgca ttgatgggta cctggaagga aaaattaaaa cagacggaaa gcttgtaatc
     4981 ggcgagaccg gtatttgcgt tggggaagct gaatgtgaga gtgtggacat cagtggcgcc
     5041 tttgatggcg tccttaaagt ctccgaactt acttcactcc gcgaaaaatg tgtctttact
     5101 ggagaacttc attcagacca gctttctgtg gaaccatccg tacgtataag cggccagctg
     5161 atttcgccgg aattgtccaa aagcaaagat aaaaaatccg ttcctcctac agtttcagct
     5221 ccaagggagc ctgccccggc cgttgccccg gaaacgaagt gacgtgatca aatcagattg
     5281 aagacgtgtt tccgcgccat tattgtctgg gctgccgaac gggccacaag gtgtgcggca
     5341 tacgctgccc atagggactg gattcctata ggattctcca aggccaggta aaaagcataa
     5401 aaagcgatga ccgatatgat catgacattg cggatagcct tgcctgcagt ggcgccaatg
     5461 tatattccat cccacatgaa agtcaggcac gatatcaggg gcatgctgta tagccaggga
     5521 aggaaaggcc gggccgcccg gatcacgtcg ggagaagagg tcatcaggcg caatagccac
     5581 tcgtcaccta ttacatagaa tactgtagaa atcacaccta ccagcgtact ccataaaaaa
     5641 aggagtttta ctgaccgcat cagggaaggc ttgtccccgg cacctatgaa ttttcccgta
     5701 agggcctctc ccgcataggc gaatccgtca acaaaatacg agaataatag caataatttc
     5761 atcatgatag tggcaacggc cagaagggta tcgccatatt gtgccgcaag gaaggtaaat
     5821 cccgtataaa tcaacaacat gcaaattgac cttatgaaca ggttaccgct cagattgaaa
     5881 taccgtttcc atcccccggt ttttatggca gcaacaaggg gaacgccacg tatcattttc
     5941 ctgtaaagca caaacagcaa caccacccca cacaggagcc cagtatactg cgctacaacc
     6001 gtaccaaggg ctatcccttt gatacccatc cccatattga gggcaaagag aatgctcaaa
     6061 ccaatattgg tgacatttat gaccacatct atgatcatag atgccagggt attctgcact
     6121 gcaatgaacc aacccctgaa cacaaaaagt gccagcaccg caggagcggc ccagatcctg
     6181 atgaaaaaat actccaggga cagggcttcc acttcgggcg aggaggggga tagcaggatg
     6241 accaggtgtg cgaagggcca ctgtatcagg atcagcagca aagaaaaggc caatgccagt
     6301 atcatgccct gcatgcctgt tcttatgatc tcctgccggt ctcccttgcc gaatgcctgt
     6361 gcggtaattc cggcagtacc aatccgcagg aacccgaaat tccaatacag caggtcaaaa
     6421 agggtggacc ctatggctat gcccccaatg gcggttgccg atccaagatg tccggcaatg
     6481 gcagtgtcag ccatgcccac caggggaatg gttattcccg caagcatgct ggggatggca
     6541 aggttgagga tctgtctgtt caggctgcgg gtcttcatgg ggaatcaggt gcggtatttt
     6601 cctccttccg gatcttccag catgcgcagg tagctatcat aacgttgtgc cggtatgagc
     6661 cctttttcga cagccgcctt caccgcacaa ccgggctctt ctgcgtgcag gcacccgttg
     6721 aagcggcagg acgcagcagc ggcgaagatt tccgggaaat aatggtacag ttcttccctg
     6781 gtcatttcca gcaggccgaa accttttatt cccggactgt ctatgatgaa tcccccggaa
     6841 cgtagcggat gcatttcata aaaggtggtg gtgtgttttc cggtcatgtg tgccagcgag
     6901 ataggggctg tccggagtct gagctccggg tctagcctgt ttgccagtgc agatttaccc
     6961 acgcccgata tacctgaaaa aagcgagatt tttcctttaa gatcctgtgc aagcatgtcc
     7021 gttccttcgc cggtttttgc cgagacttca aggacggtgt atccagccct ttgatagaca
     7081 tctttgtact gatccgcctg actgcggtat tccggagccc attccagctc gtctgtcttg
     7141 ttcaggatga tggttgcggg aaccccgtaa gcctggcatg ttaccaggaa tcggtcaata
     7201 aagggaaaag gggtttgtgg ttcggtggcg ctcaagatga tacacgccct atccagattg
     7261 gccgctatga tgtgtgcctg acgcgaaaga ttgactgatt tacggatcag gtaattcacc
     7321 ctgggatgga tctctgtgat caccgcttgt tctttcttat tgtatccgtc gggctgccag
     7381 aatccgttaa cccggtctcc cacggctatg ggatttgtca gtttatcctc ccgtaatctg
     7441 agccttccgc tgagtatggc gctgaaaggc tcccaccggg gcagaaaggt cagccggtaa
     7501 agcgaacccg tttgttttac aacggtggca ttctcaaata gtcgctgttt cataagtcct
     7561 ggtccgggcg gacaaaggta cgaaaatgct gaaaaaagac tacctttgta ttatgtttac
     7621 agtaaacgaa atacaaggat ttgtcaacca ggggcttcaa aatcttatca aatcctatga
     7681 gcacagccgc ctccacggac ctgtagaata tgcgctgtcc ggcggaggga aaaaaatgcg
     7741 tcccgtactc tgccttcttt cgtataatat cttttctgac aagcttcccg ccagtatttt
     7801 atacccatcc ctggggcttg aggtctatca taatttcacc ttgttgcatg acgatgtcat
     7861 ggataactcc gataagagaa gaaaccagcc gacggtacac aagaaatggg atgtaaacac
     7921 ggccattctg gccggtgacg ccatgtgcat gctggcatac agttatatca gcaagtgcga
     7981 gccaaaggtg ctgccccccg tgatggacgc tttccacaaa gcagcagaac aagtctgtga
     8041 aggtcaacag ctggacatgg attttgaaaa acaattattc atcacggagg atgaatactt
     8101 tgacatggta tcgaaaaaaa caggagcatt gattgcctgt tcccttcaac ttggcggatt
     8161 gtgcgcagaa gcctcctccc gttcagtaaa caaccttttc aacgccggaa tggcattggg
     8221 cagggccttt cagatccagg acgattacct ggatgtatac ggagatcccc aggtgtttgg
     8281 aaaaagtacg ggaaccgata tagccaacaa taaaaagaca tggctgctta cctatgccct
     8341 gcggaatgca acaggaaata acctggcaga actgaacagg ttgctgaccc ataaggaaga
     8401 ccccggccgt gtagagcaga tcatacagat ttacaacaag ttgagcgtag ggaagactgc
     8461 atgcgaacgt attgcgcatt acctggatac tgcgctgaac gcgctgggga gccttgatgt
     8521 ccgtgaagga aggaacaccg tactgattga tttcataaaa ggcttgattg acaggaaaag
     8581 gtaacatgaa cattgcttct ttcagtgaac attgcaacgg cctgtttgac gctgccatca
     8641 gggattacca ccggcaaaac gatacggatg ccccatacag caacccttat accgaacatt
     8701 ccctggacta cttgttatac aggaaaaacc tgatagacac tgttcaatgg catttggagg
     8761 acattatccg ggatccggcc atagagccgg cagaagcatt gcgtatcaag cgcaggattg
     8821 acgcttccaa ccaggagcgc acggatgttg tagaacagct tgacctaatg ttcaacagag
     8881 aattttcagg agttccccgt ttgcccgatg cttctctcaa tacagaaaca ccggcctggg
     8941 ctgttgaccg cttgtctatc ctggccctga aaatttacca catgaatatt gaagcagaaa
     9001 ggaaagatgc tccggcggcc tctttgtgca gacaaaaact ggaaatcctc atgatacagc
     9061 gcacggacct ttcgggggcc atagatcaat tactggccga tctgaaaaca ggcaggaaat
     9121 atatgaagct ctacaaacag atgaaaatgt acaacgatcc tcttctcaac ccgatccttt
     9181 atgaaaaaga aaaataggat accacacgta ctttgtctgc gtttttcggc attgggcgac
     9241 gtggccatcg catctattgt gctaaaagcc tgtgcccggg acaatcccag agtatttttc
     9301 accctggccg gtcctggttt acttgcccct ttgtttgcag gtgtaccaaa tttattttta
     9361 ttgtctgtag ataaaaaaca gccgctaagg atcattttta gaagcctccg gaaggttcat
     9421 cccacgcatg ttgccgacct gcatagtgtt atccggtctt ttattctgcg atctgctttc
     9481 tttttaagtg gtacacctgt tgtccggctg cacaagggac gtcgttccag gcgcaggttg
     9541 cttaaaaatc ccggccatgc acccgccctg gtgcctatgt accagcttta tcaacagaca
     9601 cttgaaaagt ctggtttcaa atctccttca ctcatcaggg ataacatcat tcccctggaa
     9661 cgcggaccct ggaaaaggat cgggattgcc ccctttgccc tgcaaaaggg aaagcaatgg
     9721 cctgctgaaa ggatgcacca ggtagcagat cggctggcca gggaaaacac cctggttttt
     9781 ctgttcggag gaggcgacga agagatggct cacatgaaag cctgggcagc cgggaacagc
     9841 aatattcgcc tggccgccga cgcaggaagt gattttgagg ctgaactggc ggtcatcaag
     9901 accctggatg tgatgatcag catggactcg gccaatatgc actttgcctc ggccatgggt
     9961 attcctgtca tatccatctg gggagccacc catcccaaag caggttttta cggatggaga
    10021 caggatccgg acagggcgat acaattaccc atggattgcc gtccatgttc catattcggg
    10081 gccaagccct gctggaaggg cacctatgcg tgcatggaag accttaccgc tgaacaagtg
    10141 tattcgtatg tcaggtctgt catggaataa accggagatc atggctccgg cgggcaactg
    10201 ggccagcctg actgccgccg cgcagggagg tgcccatgcg gtctattttg gagtgggtga
    10261 actgaatatg cgtgcgcatg cggcacataa cttcagaagg gaagacttgc ctgagataac
    10321 ggcattctgc cgcaggaacg gcatgcgttc ctaccttacc gtcaatacca tcttttacga
    10381 ccaggacctg gaagcgttgc atgccatttt acaggcggct gcagatgccg gggtatcggc
    10441 tgtcattgca tcggatatgg cagccatcga ggaagcccga tctctgggac tggaggtgca
    10501 tatttccacg cagctgaaca tatccaacat cagggcgctt gcattttatg ccaggtttgc
    10561 cgatgtggtg gttctggcca gggagctgac gctggaccaa atcagcgcca tcgcccggga
    10621 agtgaaggag cgcaacatta ccgggccttc aggaaacctt gtcaaactgg agcttttctg
    10681 ccacggtgcc ctgtgcatgg ctgtatcggg caaatgttac atgagcctcc accagtacaa
    10741 taaatcggcg aaccggggcg cctgctacca ggtctgccgc aggtcctata cggtcacaga
    10801 caaagagacc ggagaagcca tggaagtgga caaccagtac atcatgtccc ccaaagacct
    10861 gtgcacggtc cgtttcctgg acaagatact tgacagcggc attgagatcc taaagattga
    10921 aggccgggcc cgttctccgg aatacgtgaa aaccactgca caagtctatt ccaaagcggc
    10981 tgaggccgtg gttaacggaa cattcacacc tcaactggca gccggtctgg aagagcagtt
    11041 gaaaactgtg ttcaacagag gattctgggg cgggtattac ctgggagaat ccctgggaga
    11101 gtggagtgaa acatatggga accaggctac acaaaaaaaa acgtatgtgg cccggataac
    11161 caattatttt gccaggcaag gcgttgccca gttaaaagtg gaggccgtcc cgttgcatgc
    11221 aggggacgaa atcatgatca tcggccccac taccggggtg gttgaggaaa gggtacgcga
    11281 gatccgtctg gagggaggtt cagccgtttc catggcaccg aagggaactg tctgctccat
    11341 tcctgtgact gtccggttac gcaggtcgga caaagtatac cgcctggaga aaaatgacta
    11401 gactatttcg gcgatcatac agcgggcaga gccgccgccg tgtgtttcta tggtatccag
    11461 ttccggagtg atgagtttgt tgaaggacct gagaatcttt cgttgtgaag gagtgaggac
    11521 tttgtaagcg cgttttgaca tgaccaggaa gggcttgtcc ttacgtgaaa aaacctggaa
    11581 catattgccg gcaaagtggt tcatttgttt cgccgtgatc tcgaccaccg ttttgcggtc
    11641 ctctatgaaa acatccagaa gcatctgcct gtcagcctca gaaggaatgg attccaggca
    11701 gatgaccaca tagtcgcggg ccatgcacat catcacgttg gtgtggtaaa tggcggatcc
    11761 cttttccagg gcatcaaaac aaattgcctg gtagcccaat gtatcacaga tatcccggaa
    11821 cagaccctca tcggtcctgg gagagcggca ggcataggcc ctgttgttgt cccggtcaaa
    11881 gaccatgctc cctgtcccct ccaggtaacg gttttcacgc tcccgggggc tcatgtctat
    11941 ccattttatg cgcggggcat gttcctgaat ggccctaaag acagtttttt tccgttccag
    12001 ccgcctgttg ggagcgtaca tggggtacaa gacagacatt tgtcccgggt gaaaagatat
    12061 ccagttgttg gggaatatgg aatcgggcgt gtgcggctcg ggtgtgtcct gaaccactat
    12121 cacgtcaata tcgtgttgct gcaacagggt aacatagctg tcgaattctg caagagcctt
    12181 ttcctgcacc agcccggaat cttctcccgt ggctacctgg aaggcattat tgactgctgt
    12241 ttgcggattg aacccgaaac agcaggggcg tatcataagc agttttttat tcaaaactca
    12301 gaatggtttt acgaatgatt tccagggcct gcatcagttg cgattctttt atgaccaggg
    12361 gcggtgccag gcggattata tgcctgtgcg taggtttggc caacacaccg ttttttgcca
    12421 tggccataca gatatcccag gcttccaccc cgtttttagg ggtaatgaca atggcgttca
    12481 gcaatccctt gccacgcacc agttcaatgc ggtctgaagg gatcgatctc aggaaatttc
    12541 ttgctatttc tcccaggtaa gtggctttct cggccagttt ttcgtcttcc accacattga
    12601 gggctgcatg agccaccctg caggcaagcg gaaatcctcc gaatgttgag ccgtgctctc
    12661 cgggttttat ggtgagcatg atctcgtcgt ttgagagaac ggcagagaca ggaagcacgc
    12721 ctcccgagat ggccttgccc aggatcagga tatcgggatg caccccttcg tgatcacagc
    12781 agatacgttt gcccgtgcgg gcaatgcccg tctggacctc atccgctacg aatagcacat
    12841 tgtgttcctt gcaaaggtca tagcactttt tgatataccc ctcatcaggg accatgaccc
    12901 cggcttctcc ctggatgggt tcaacaagga atccggcaac gtgcgggcca tattccctga
    12961 ggactttctc caaagcattg ggattgttgt aagggatctt gataaaacca ggggtgaacg
    13021 gaccgaatcc ctcataggcg gaggaatctg tggaaaggga gatcaccgtg atcgtgcggc
    13081 cgtgaaaatt tccttcacag caaatgattt tggcttcgtt cgcaggaatg ttctttttat
    13141 cataccccca gcgacgggcc agtttcaagg ctgtctcaac ggcttcggcc ccggtgttca
    13201 tggtcagaac cttgtcgtat ccgaaaaaac gggtgatata ttcttcataa ggtcccaggc
    13261 tgtcgttgta aaaagcacgg gatgtaaggc ataactgttt ggcctgatct gtcagggcct
    13321 tcacaatctt tgggtgacaa tgtccttggt tcacagcgga atatgccgaa agaaaatcaa
    13381 aatacttttt tccttcaata tcccagacat agggaccttt gcccttgctc aggacaaccg
    13441 gaagcggatg ataattgtgc gctccgtatt cttcttctaa tgtgatataa tcttgagatt
    13501 tcatggttgc gaagatatgg aattttattg cactgtaaa
//
LOCUS       DAOSOF010000046        13307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4685_length_13307_cov_4.449291, whole genome shotgun sequence.
ACCESSION   DAOSOF010000046 DAOSOF010000000
VERSION     DAOSOF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4685_length_13307_cov_4.449291"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLM68_04015"
     CDS             complement(<1..1615)
                     /locus_tag="PLM68_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT54563.1"
                     /translation="MVKRIIQRTLMTMAALMLVISAFAQSRTFCNPIDLLAGNQRASR
                     GGEPVVLIYQDDYYLFVTGTRGYWYSPDFLDWKYIDSPTFPGGVISVIEKDGILYACS
                     MNNKNVYKCEEPKKGEWTLTATFDSDRYGDANMFLDDDGRLYLYYGWSQIMPFKVVEL
                     DVNTFKEISEPKVLFFGDYKNHGFENRRNQDVIFSIFNGRREYFNEEFPWIEGPWVTK
                     HNGKYYLQYAAIGLEFLSYSHGVYVSESPMGPYTYSEHNPLTFKTTGFAVGAGHGSTF
                     HDKNGQLWTICMIPAWYAGNGGAELAIYPTAVDDDGVMYSNTAYGDYPQYWPGIRTDA
                     VDSNQAGWLLLSHKKRIEVSSTYDNYVAANAADEDFMTCWSAQTGDPGEYLTVDLGKV
                     SDIHAIQCNFDHIGSTVNVGRGFRPSPAPDHYQCYTVEVSNDNATWKTVIDKSDNKFD
                     RRHDYTEFSNPVKARYVKVTNVATHDGAKFAVKDLRVFGNPNAAERVVVDDFKAIRNQ
                     EDRREATLLWEPVKGADGYVVRYGIEPGKL"
     gene            complement(1646..4219)
                     /locus_tag="PLM68_04020"
     CDS             complement(1646..4219)
                     /locus_tag="PLM68_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT54564.1"
                     /translation="MKSKFLLVSFLILSVFFPKAQAQNFHINDLEYFEERGVNLLVYS
                     NKYAGMFCDEKLAAIELIQRGERISTGGGIRLMNTPEQWDIFPELVNRTVDRTENSIE
                     VELCYKEYNFTSKVKVTSSGKGFKIAVYLDRPVPEILVGRAGLNWEFFPATFFGKTYM
                     MDGIPGILPKYPADNTYMLPRSEKIEQFYKLSTFDDRGRNEFIVPHPFSKGNKLVLAP
                     EDADVRVSISSESEINLFDGRNLAQNGTYVVRSYIPANKTGKVLEWYVEPSYDAAWVR
                     EPNVGFSQIGYTPAQKKVAVVELDKNAAPVETADIIRISDKGDEVVAYKAPVKPWGVF
                     NNRYNYATIDFTAVQESGLYCICFGGFRTNVFPINKDVYNGKWHASMDVFLPVQMDHM
                     EVNEGYRTWHGRSHMDDALQAPVNYEQFDGYRQGAETNTKYKSWEHIPGLAVGGWYDA
                     GDFDIQSRTVVSMAADLITLWDLFKPERDQTFIDQDTKFVDIHRPVGTPDVIQQIQHG
                     VLNIIAQVENIGFVAQGIVQSNMYQYHHLGDASTITDGLVYNPSLKPYETDGLTSGTR
                     DDRVAFTGNFSAAGTMSTVASLASAARVLKPYYPKVADRALRNAIMLWEKYSEDADPA
                     KIKNPMMRRFGGDGRVNASIELWRTTGDNSYVKFFEEAVLKQLEPIEMNFPGAPAGKF
                     YNLGTALSLYPLMDKEFQDKVKAAIPGYVESLKETASQNPYGVPISGRGWGGNDQVIR
                     WAYNNYLVWKYFPDMIDPELVLLGLNYLYGCHPYSNVSFITDIGVNTKNVSYGNNRAD
                     YTVIPGGVVPGLLLLAPDYMENKDDYPFLWGENECCTNSIPNFVMLSLASEEVAAALN
                     K"
     gene            complement(4249..6138)
                     /locus_tag="PLM68_04025"
     CDS             complement(4249..6138)
                     /locus_tag="PLM68_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOT54565.1"
                     /translation="MKRLFLSLTLILLAFTGNAQDKNFHIFLCFGQSNMEGAARIEPQ
                     DTVNISKRFRMMAAVNDASRGRVKGEWYQVVPPLCRERTGLTPVDYFGRTLVEVLPED
                     HTVGVINVSIGGCRIEAFMKDSIGAYAKTAPQWMIPALKAYDNDPYKWLVELARKAQK
                     DGVIAGILMHQGESNIGEEDWPLKVKSVYENLLKDLDLKAENVPLIAGEVVNADRGGR
                     SASMNAIIDKLPLVIPTAHVVSSSCCTQFFDQIHFDAKGYRDFGRRYAAVMLPLMGYK
                     APVSRMYNLPVESPVIHRFGGGVTFNIRAPHAKSVLLSSQFLKENVSLTMNREGIWSV
                     TLRVENPDIYPYNFIVDGVTVSDPSNTELFPNEGFKASLLTVPDEKALYHVNDVPHGR
                     VTWCTYKSDVLGTYRPLLVYTPAGYETGDRSYPVFYLVSGTTDTEETWFKVGKVNTIL
                     DNLIAQGQANPMIVVMPYGNMMIGTPMPSSLQAAEMYGVFAKEMTECIMPYVEKEFRT
                     LPGRDNNAIAGFSRGGGQSLFTAFSHMDQFAWVCSYSAYLTPEVMDLYFPFFTKSPDD
                     INAQFKLMWFGVGEDDFLYRDVVKNREYFDTRGISYQSLTTKGGHTWMNARTYLSTTL
                     QLLFK"
     gene            complement(6145..7293)
                     /locus_tag="PLM68_04030"
     CDS             complement(6145..7293)
                     /locus_tag="PLM68_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT54566.1"
                     /translation="MRLSALSVIGAVFLMLTACNSNPRPGERIREVIQPGEQAPFADP
                     GQRNFGGFQRRDTTGKAPAPRPRFKMPEVSIVHFNDLVMSDPFIYPHPETKTYYLTSS
                     GGWMYKSQDLKMWTGPFKIIDIEGLWLEKAGFAAAAEIHRIGDYYYYAGTWSDHSDLI
                     EQVPRRYNVPHNQTYLLRADNPEGPYTSFAIEPGYDYQPRDWDCIDGTLYEENGKIYM
                     IFVHEWTQLIDGTMDYIELAPDLSYTVSRKPVTMFRASEAPWSMEMNSIGEATFGLKM
                     PGWVTDGPQMFRTQTGKLGMLWSSWGKERYVQGIAYSESGTIAGPWVQEKEAFLSNNS
                     GHGMLFRTFEGKLIFLVHHAAENGPRKPQYWNVDDSGDKLILGSQINI"
     gene            complement(7417..9093)
                     /locus_tag="PLM68_04035"
     CDS             complement(7417..9093)
                     /locus_tag="PLM68_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54567.1"
                     /translation="MKKYIIPILLLTLGLSSCNKLLEIDQKGVISTDSFYQTDADAES
                     ALVAAYENASYGLVRHNDYIYSPYNALLTYPSDDIYAAGAFYGDNDFLAQLTEFRYDI
                     NNQAISNQYKGFYKVIYGANLVIDNFEYGASAVKDRCISEARVLRAFSHMMLAILWGT
                     PPIVDHVLTGADRPGNSESQEAVLRWAAAECIDAAKYLDERQSTADKKGTAKITKGAA
                     YAFAGKALMFAKDYSAAKTELKKVISSGKYALVPGNRIGENFHVAGDGNEEKIFELNH
                     VYNSNIGTWSGRIQRSTWMQINLWNWRADRLGGTPTHLFGGGWGMIHPTAAFAEALIA
                     NDGYDSYRRKAWLLTYDEVLYDMPYLSDSDNPTREDKEKDPRRGIVEPVGLYGCEGYF
                     HLKMIALASDVIQGSYSDVNFLIMRYAEVLLMYAECCAQTNDNDGLQYLNMVQQRAGS
                     QTISTALTLDAVKKEKQFEMWMEGCRFIDLLRWGEAAAKLAKNGDSIPTFKDKFATEN
                     SGKHEGFVDWSNSNVNQGKEHGFKAGKHELYPFPFSETSINENIVQNPGW"
     gene            complement(9113..12211)
                     /locus_tag="PLM68_04040"
     CDS             complement(9113..12211)
                     /locus_tag="PLM68_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54568.1"
                     /translation="MIKKLLNVVKIALILAACVTSIGSYAQTRAISGKVIDAEGNPII
                     GASVVVVGNDRIYTVTDLDGQFSLQVPTGANLRISFIGYVSQVIPIGDQRTFEIAMVE
                     DMELLEEVVVVGYGVQRKSDVTGAVSSVREVDLANRTTSDAASALQGKAAGVQILNYS
                     AAPGAGSQIRVRGYSSNSSSGLGPLLIVDGLQVDNIQYLDPQMIESMEVLKDAASAAI
                     YGAQAGNGVVLITTKTGRKMGGGQVFYNFQNVWNSLGKAPEVMNAEQYIDYKIAEGVL
                     TQQRLNELGYDGTDTNWAKEVFTTSLTKRHTVGFMGGNDRGQFYTALSYVSDDGIVKG
                     DKDLYERLSAQINADYKIKDWLTVGTNTSIEKWKTRSVSQHTEYGSFMMGTLTQDPLT
                     PVYFPTYNDLTQDLKNHYDAGDNILGDENGFYAVSRYAETDSGHPFVQRDKEDVENQG
                     ISVRGTLFANLTPIKGLTITSRFGYRIYQSYYTRYAPPYYANSMAYKTNYDIQANANH
                     GFYYQWENFANYTFNVLKNTFTLMGGMSYIQNNTFGVNGTGTGPNPLQGYAPNFRYLS
                     YLNNNPDTSKTVTSSPGVSSNLSYFGRIHWNYADKYNIQVNFRADAFDSSKLPVDARW
                     GYFPSVSAGWVLTNESFMQGISKEALSFLKIRGSWGVNGNIAVLNNYPYATTISYNAN
                     YYHYDPASPNVTYGSSPSGLANPNLRWETSVQWNVGLEARFLNNRLSLAADYYEKRTD
                     DLLVSIAPVAEVGVGSSTINAGEVMNKGVELELEWKDSKGDFFYSINANMAYLNNKVT
                     FLDPTISRITGMSAPQGYETFSAFEVGYPVWYMRGYQYEGVDSATGKPIIADLNGDGQ
                     INNLDHTMIGCGIPDLTYGLTIKLAWKGFDFTLFGTGTYGNEIFAIHHRTDRLFYNSL
                     EYFHKNAWTESNKNASMPSAISVATDAQFWGSSASIFDGSYFKIKQIQLGYTLPRKWT
                     QKIFISSLRIYASLDDYFTFSKYPGMDPETAHAGNANSMGYDKGSYPTRKKAVIGINI
                     AF"
     gene            12576..>13307
                     /locus_tag="PLM68_04045"
     CDS             12576..>13307
                     /locus_tag="PLM68_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOT54569.1"
                     /translation="MKQFILTVCLLAAVCLKASGIEQYYFKHYKSEDGLPNNTVTCSI
                     QDHFGFLWFGTKDGICRFNGNDFTVIDDPDLRSSINSMTLALCEDHDGLLWYYSTNGT
                     GFYDPYTDKITSVKELDGHRFFDIDVDKDNNVWFISSNSIFKLDRSTGSYVRYSSERN
                     FTALHSCIDKEGNIWFTTYEGDVRRYNPRTDYFEVFPVITEKTMTSDTYPVYIEAIND
                     TKLLISTNHYDLIRLDIDSGNCDYLT"
BASE COUNT         3592 a         3108 c         2908 g         3699 t
ORIGIN      
        1 atagctttcc aggctctatg ccgtaacgca ccacataccc gtcagcccct ttgacaggtt
       61 cccagagaag ggttgcctcg cgccggtctt cctgattccg tatagctttg aaatcatcaa
      121 ctacaacacg ttcggctgca ttgggattac cgaatacccg gaggtccttg acagcaaatt
      181 tggcaccatc gtgagtggct acattggtga ccttcacata acgggccttc accggattgg
      241 agaactctgt gtaatcgtgc ctgcgatcaa atttattgtc actcttgtca attacagtct
      301 tccaggtggc attatcgttc gatacttcaa ccgtatagca ctggtaatgg tccggagccg
      361 gagacggtct gaatccgcgt ccgacattta ctgtggagcc tatgtggtca aagttgcatt
      421 ggatagcatg aatgtcactt acttttccaa ggtcaacggt aagatactca ccaggatccc
      481 ccgtctgtgc tgaccagcat gtcataaagt cctcatcggc tgcattggca gctacatagt
      541 tatcatatgt tgaagaaact tcaatacgtt tcttatgaga aagcaacagc cagccagctt
      601 ggttactatc aacggcatct gtcctgatac cgggccagta ttgcgggtaa tcaccataag
      661 ccgtattgga atacatcacg ccatcatcat caactgcggt aggataaatg gcaagttcag
      721 ctccaccgtt gcctgcatac caggcaggga tcatgcatat agtccatagc tgaccgttct
      781 tatcgtggaa tgtgctccca tgaccagcac ctacggcaaa acctgtggtt ttgaatgtaa
      841 gcggattgtg ttccgagtag gtgtaagggc ccattggact ttctgataca tatacaccat
      901 gagaataaga aagaaactca agaccaatgg ctgcatactg gagatagtat tttccgttgt
      961 gtttggttac ccagggacct tcaatccatg gaaattcctc gttaaaatac tctcttctcc
     1021 cattgaagat cgagaaaatc acatcttgat tccttctgtt ttcgaatccg tgattcttgt
     1081 aatccccaaa gaaaagcacc ttgggctccg agatttcctt gaaggtgttc acatccagtt
     1141 ccacaacctt gaacggcatg atctgtgacc atccgtaata gagataaagc cttccgtcat
     1201 catccaggaa catgttggca tcaccgtagc gatcactgtc aaaagtagcc gtaagcgtcc
     1261 actccccttt ctttggttct tcgcatttgt aaacgttctt attattcatg gaacaggcat
     1321 agagaatgcc gtccttctcg atcacagaga tcacccctcc cggaaaggta ggtgaatcta
     1381 tgtatttcca atccaggaaa tccggggaat accaatatcc cctggtccct gtgacaaaaa
     1441 gatagtagtc gtcctgatag atcagcacca ccggttcccc accgcggctg gcccgctgat
     1501 ttccggcgag cagatcaatg ggattacaaa atgttctact ctgagcaaag gcagatatga
     1561 caagcatcag ggctgccatt gtcatgagtg ttctttgtat tattcttttg accattatat
     1621 gataggtgta atgttagtaa taatcttatt tattgagtgc ggcagctact tcttcactgg
     1681 caagggacaa catcacgaaa ttgggaattg agttggtaca gcactcattt tcgccccaga
     1741 ggaacggata atcatccttg ttttccatgt aatccggtgc aagaagaaga agacccggaa
     1801 cgactccgcc ggggattacc gtatagtcgg cgcgattatt tccataagaa acgttcttag
     1861 tattgacgcc tatatccgta ataaaagata cattcgagta cggatggcat ccatacaggt
     1921 aattcagacc aagtaatacc agttccggat caatcatatc gggaaagtat ttccaaacca
     1981 ggtagttatt ataagcccac ctgattacct ggtcatttcc gccccatcca cgacccgaaa
     2041 tgggtacacc gtaaggattc tgggaagcag tttctttaag agattccacg tatccgggaa
     2101 tagcggcctt cactttatcc tggaactcct tgtccatgag aggatagagg cttagggccg
     2161 tgccgaggtt gtagaatttg cctgcgggtg ctccggggaa gttcatttca atgggttcaa
     2221 gttgcttgag aacagcctct tcgaaaaatt tcacataact gttatcacct gttgtcctcc
     2281 aaagttctat ggaagcgtta accctgccgt caccgccaaa ccgtctcatc attggattct
     2341 tgattttggc aggatcggca tcttctgaat atttctccca aagcatgatg gcattcctga
     2401 gtgcccggtc tgcgacctta ggataatagg gtttaagaac ccgggctgcc gaggcaaggg
     2461 atgcgacagt ggacatggta ccggcagcac tgaagtttcc ggtaaatgcg acacggtcat
     2521 cacgggtgcc tgaagtaagg ccatctgtct catagggttt cagagaagga ttatagacaa
     2581 ggccgtctgt tattgtagag gcatcaccca gatgatgata ctggtacatg ttgctttgaa
     2641 ctatgccctg agctacaaaa cctatgtttt ctacttgtgc aatgatgtta agtacaccat
     2701 gctgaatctg ctgaattacg tcgggcgttc cgaccggacg atgtatgtct acaaatttgg
     2761 tatcctggtc aatgaacgtc tggtccctct ccggcttgaa aagatcccat aaagtgatca
     2821 ggtcagcagc cattgaaacg acagttctgc tctgaatgtc aaagtcacct gcatcgtacc
     2881 agccaccaac agcaagtccc gggatatgtt cccatgattt atattttgta ttggtttcag
     2941 caccctgcct gtaaccgtcg aactgttcat aattgaccgg agcctgaagg gcgtcatcca
     3001 tgtgagaacg tccgtgccag gtacgatatc cttcgttcac ttccatatgg tccatctgaa
     3061 ccggaagaaa tacatccatg gacgcatgcc atttaccatt gtatacatcc ttgtttatgg
     3121 gaaatacatt ggtcctgaaa ccgccaaaac aaatgcaata aagacctgat tcctgtacag
     3181 cggtgaaatc aatagtagca taattgtacc ggttgttgaa tacgccccat ggcttgaccg
     3241 gagctttgta agccaccact tcatctccct tgtcggaaat acggataata tctgctgtct
     3301 ctacgggagc tgcattcttg tcaagttcca caacggctac tttcttctga gccggagtat
     3361 agccaatctg cgagaagccc acattgggtt cacgtaccca ggcggcgtcg taagaaggct
     3421 caacgtacca ttcaaggacc tttccggttt tgttggcagg aatatatgac cggaccacat
     3481 aagtgccgtt ctgggcaaga tttctgccgt cgaaaaggtt tatttcagat tcggaagaaa
     3541 tggatacgcg aacatcggcg tcttcaggag cgagaaccag tttgtttccc tttgagaaag
     3601 gatggggaac aatgaactca ttacgtcctc tgtcatcgaa agttgaaagt ttgtagaatt
     3661 gctcaatctt ttcgcttctg ggaagcatgt aggtattgtc tgccgggtat ttgggcaata
     3721 ttcccggaat cccgtccatc atatacgttt tgccaaagaa agtcgcgggg aaaaactccc
     3781 agttgagacc ggcacggcca accaatattt ccgggacagg ccggtccaga tacacagcga
     3841 tcttgaaccc tttgcctgaa gaggttactt tcactttaga agtaaaatta tattctttgt
     3901 agcaaagttc cacttcaata gaattctctg tacgatctac ggttctgttg accagctccg
     3961 ggaaaatatc ccattgctcc ggagtgttca taaggcggat gccgcccccg gtactgattc
     4021 tctcgccacg ttgaattaat tcaatagctg caagtttttc atcgcaaaac atgcctgcat
     4081 atttattact gtaaaccagt aaattaacac ctctttcctc gaaatactcc agatcgttga
     4141 tatgaaaatt ctgtgcctgt gctttcggga aaaatacaga gaggatcaga aaggatacta
     4201 atagaaattt ggatttcata acgggcggtt tttcataggt ggaaatattt atttaaaaag
     4261 taattgcagt gtggtggaca gatatgttct cgcgttcatc catgtatgac cgccttttgt
     4321 tgtcagcgac tggtaggaga ttcccctggt atcgaaatac tccctgttct ttacaacgtc
     4381 cctgtaaagg aaatcatctt ctcccactcc aaaccacatg agtttgaatt gagcattaat
     4441 gtcatcggga gatttagtga agaacgggaa atagaggtcc atgacttcgg gagtaagata
     4501 agcgctatag gaacagaccc aggcgaattg atccatatgg gagaaggcag tgaataatga
     4561 ttggccgcct ccacgcgaga atccggcaat agcattgtta tcgcgtcccg ggagcgtacg
     4621 aaactctttc tcgacatagg gcattatgca ttcagtcatt tcttttgcaa atacaccgta
     4681 catctctgct gcctgaagcg acgaaggcat tggcgtccct atcatcatat tgccgtatgg
     4741 cattaccacg atcatgggat tggcctgtcc ctgggcaata aggttgtcaa gaatcgtatt
     4801 gactttaccc accttgaacc atgtttcctc tgtatctgtg gttccactga ccagatagaa
     4861 aacgggatac gatctatccc cggtttcgta tcctgccgga gtgtaaacca ataaggggcg
     4921 gtatgtgcca agaacgtcag atttataggt gcaccatgtc acgcgtccgt gcggaacatc
     4981 gttaacgtga tacaaagcct tttcatcagg aacggtaagc aggctggctt tgaacccttc
     5041 gttagggaat aattctgtat tggagggatc agaaacggtt acaccgtcta caataaaatt
     5101 ataaggataa atatcaggat tctctaccct aagtgttaca ctccagatgc cctccctgtt
     5161 catagtcaac gaaacgtttt ccttcaggaa ctgtgaagat aaaagaaccg atttggcatg
     5221 aggggctctg atattgaatg tcactccgcc tccaaaacga tggataacag gagattctac
     5281 gggaagattg tacatccgtg aaaccggagc cttgtatccc atcagtggaa gcatcactgc
     5341 cgcatagcgt ctgccaaaat cgcggtagcc cttagcatca aagtgtatct ggtcaaagaa
     5401 ctgagtacag caacttgatg aaactacatg ggcagttgga ataaccagtg gcagcttatc
     5461 aataatggcg ttcatgcttg cagaccttcc tcccctgtca gcattgacca cttctccggc
     5521 aataagagga acattttctg ctttaagatc caaatcctta agaagattct cataaacgct
     5581 tttcactttg agaggccaat cctcttctcc aatgttcgat tcaccctgat gcataagtat
     5641 tccagcaata actccgtctt tctgagcttt acgggcaagt tctaccagcc acttatacgg
     5701 gtcgttatca tatgctttaa gtgcagggat catccactgc ggagcagtct ttgcgtaagc
     5761 acctatcgaa tctttcatga aagcttcaat ccggcatcct ccaatggaaa catttatgac
     5821 tcctacagtg tgatcttcag ggagaacttc caccaatgta cgaccaaagt aatctaccgg
     5881 tgtgagtcct gtcctttctc tgcaaagcgg tggaaccact tggtaccatt cgcctttcac
     5941 acggccgcgg gacgcgtcat ttacagcagc catcatgcgg aaacgtttac ttatattgac
     6001 ggtatcctga ggctcaattc tggcagctcc ttccatattt gactgtccaa aacaaaggaa
     6061 aatgtggaaa ttcttatcct gtgcattacc tgtgaaggca agaagaataa gtgttaatga
     6121 taaaaacaat cttttcataa tagatcagat atttatttgg gaacctaaaa taagcttgtc
     6181 cccggaatca tctacgttcc agtattgcgg cttcctgggt ccgttttcag cagcgtgatg
     6241 caccagaaag atcagtttac cttcaaatgt tctgaacagc atgccgtgac ctgagttatt
     6301 tgaaaggaac gcttcttttt cctgtaccca ggggccggct atggtaccgg actcggagta
     6361 ggctattccc tgaacgtatc tttctttacc ccagctagac cagagcatcc ccagcttacc
     6421 ggtttgtgtc cggaacatct ggggcccgtc tgtgacccaa cccggcattt tcagaccaaa
     6481 agtggcttca ccaatggaat tcatttccat cgaccaagga gcctctgatg cacggaacat
     6541 ggtaaccggt ttcctggaaa cggtataact caggtcagga gcgagttcaa tgtaatccat
     6601 tgtaccgtca ataagctgtg tccattcgtg tacaaagatc atatagatct tcccgttctc
     6661 ttcatagaga gtcccatcta tacaatccca gtcacgaggc tggtagtcat aaccgggttc
     6721 aatggcaaaa gaggtatacg gaccttcggg attatctgcc ctgagcagat aggtttggtt
     6781 atgaggaaca ttgtatctcc ttgggacttg ctcgataaga tcactgtggt cgctccatgt
     6841 gccggcataa taatagtagt ctcctatcct gtgaatctcc gctgctgcgg caaatccggc
     6901 tttttccagc cataatcctt cgatgtcaat aattttaaaa gggcccgtcc acatttttag
     6961 gtcctgactt ttatacatcc atccaccgga actggttagg taataagtct tcgtttccgg
     7021 atgcggataa atgaacggat cgctcatgac caggtcgttg aaatgtacta tactgacttc
     7081 aggcatcttg aatcttggac gtggcgcagg agccttgccg gttgtatctc tcctctggaa
     7141 accaccaaaa ttcctctgtc cgggatcggc aaacggcgct tgttctcccg gctggatcac
     7201 ttcacgtatt ctttctcccg gccgtggatt ggaattgcat gcggtaagca taaggaatac
     7261 ggcccctata actgacaatg ctgataatct catgggataa ttgtgtttgt aatattatta
     7321 tggtatcatt aaatcagata taaatggagt ggaccccggt gatggaatcc actcccgcac
     7381 aaaaaaacct attggaaacc taaccaaaac ttaaacttac caaccaggat tttgtactat
     7441 gttttcattg atagacgttt cgctgaaggg gaaaggatac agttcatgct ttcctgcctt
     7501 gaaaccgtgt tcttttccct gattcacatt cgaatttgac caatcaacga aaccttcgtg
     7561 cttgccactg ttttctgtag cgaatttgtc tttgaacgta ggaatgctgt ccccgttctt
     7621 ggcaagtttt gcagcggctt cgccccaacg tagcaggtca atgaaacggc aaccttccat
     7681 ccacatttca aactgtttct ccttcttaac cgcatcaagg gtaagggcag tacttatggt
     7741 ttgagaacct gctctctgct gcaccatgtt cagatattgg agtccatcgt tatcgtttgt
     7801 ctgggcacaa cattctgcat acatcagaag cacctctgca tacctcataa tcagaaagtt
     7861 aacgtctgaa tacgaacctt gtatgacatc agatgcaaga gcaatcattt taagatggaa
     7921 ataaccttca catccataca gacccacagg ttctacgata ccgcgacggg gatctttctc
     7981 cttatcctca cgtgtgggat tatcactatc gctaagataa ggcatatcat aaagaacctc
     8041 atcataagtt aagagccatg ccttgcggcg atacgaatca taaccgtcat tggctatgag
     8101 tgcttcggca aacgcagcgg taggatggat cattccccag cctccaccaa agaggtgggt
     8161 cggtgtgcct cccaatctgt cagcacgcca gttccaaaga tttatctgca tccaggtgga
     8221 acgctgtata cgaccactcc aggtccctat gttggaattg tacacgtggt ttaattcaaa
     8281 gatcttttct tcattgccgt cacctgcaac atggaaattc tcgccaatcc tgtttccggg
     8341 aacaagggca tacttgccgg aagaaataac ttttttcaat tcagtcttag cggcagagta
     8401 atctttagca aacataagtg ccttaccggc aaaggcatag gcagcacctt tggtgatctt
     8461 ggcagttcct tttttatctg ctgtgctttg tctttcatcc agatatttgg cagcgtcaat
     8521 acactcggcg gcagcccatc tgagaacggc ttcctggctc tcggaatttc caggccggtc
     8581 cgcaccggtc agtacatggt caactatggg gggagtgccc cagagaatgg caagcatcat
     8641 gtgactgaaa gcacgaagaa cccttgcttc tgaaatacac ctgtccttta ctgcacttgc
     8701 gccatattca aaattatcaa ttaccaggtt tgccccgtag atcactttat aaaaaccttt
     8761 gtattgatta ctaatggcct ggttgtttat gtcataacgg aactcagtca gctgcgcaag
     8821 aaaatcgttg tcaccgtaaa aggctccggc agcgtagata tcgtcagacg ggtaagtaag
     8881 caatgcattg tatggagagt agatataatc gttatgtctg actaatccat aactcgcatt
     8941 ctcgtaagca gcaaccaacg cagattcggc atcggcatcg gtttggtaga aggagtccgt
     9001 ggaaatgact cccttctgat caatctcaag gagtttgtta caagaactca aacctaaggt
     9061 tagtaacaaa atgggaatga tatatttttt cataataatc taaattgggt tattagaatg
     9121 caatgttaat acctatcaca gctttcttcc tggtaggata ggaccccttg tcatagccca
     9181 tggaattggc atttccggcg tgtgctgttt caggatccat accgggatac tttgagaaag
     9241 tgaaataatc atccagagaa gcatagattc tcaaactgct aatgaagatc ttctgtgtcc
     9301 acttgcgggg aagcgtgtaa cctagctgga tctgtttgat cttgaagtat gatccgtcga
     9361 atatcgaggc acttgagccc cagaattgtg cgtcagtagc cacactgata gcactgggca
     9421 tggaggcatt tttattggat tctgtccagg cattcttgtg gaaatactcc aatgagttgt
     9481 agaacagacg gtcagttcta tgatgtatgg caaagatctc attaccataa gtacccgtac
     9541 cgaataatgt aaagtcaaaa cccttccatg caagtttgat ggtcagacca taagtcaggt
     9601 caggaatacc gcaaccgatc atcgtatggt caaggttatt aatttgaccg tccccgttca
     9661 gatctgctat gatcggttta cctgttgcgc tatccacccc ttcgtattga taacctctca
     9721 tataccaaac cgggtatccc acctcaaacg ccgagaacgt ctcatacccc tgaggtgctg
     9781 acatacccgt aatcctggaa atggtgggat caagaaatgt taccttatta ttaaggtatg
     9841 ccatgtttgc attgattgaa taaaagaagt ctccttttga atctttccat tccagttcga
     9901 gctctacccc tttgttcata acctctccgg cattgatggt agaagatccc actcccacct
     9961 cggcaactgg agcaatactg acaagaaggt catcggttct cttttcgtaa tagtcagcag
    10021 ccagggaaag tcggttgttc aggaaacgtg cctccaaacc aacattccat tgaacagaag
    10081 tttcccacct aaggttaggg ttcgcaagtc cggagggact ggatccgtaa gttacattag
    10141 gagaggcggg atcataatga taatagttgg cattgtagga aatggtagtt gcataaggat
    10201 aattgttcaa tacggcaata ttaccgttca caccccagga tccgcggatc ttaagaaagg
    10261 agagggcctc tttgctgatc ccttgcatga atgattcatt ggtaagcacc caacctgccg
    10321 atactgaagg gaagtatccc cagcgggcat caactggtag ttttgatgaa tcaaacgcat
    10381 ctgcacggaa gtttacctgg atattatact tgtctgcata attccagtgg atacgtccga
    10441 agtaagaaag attggaggat actccgggag aacttgtcac agtttttgat gtatccggat
    10501 tgttgttcag ataagacaaa tagcggaagt tcggagcata tccctgaaga ggattaggac
    10561 cggtacccgt tccgttgaca ccaaaagtgt tgttctgtat ataagacata cctcccatga
    10621 gggtgaatgt attcttcaga acattgaaag tatagttggc gaagttttcc cactggtagt
    10681 agaatccgtg gttcgcattg gcctggatat cataattagt tttataagcc attgagttgg
    10741 cataatacgg aggggcatac ctagtgtaat aactctgata aattcggtat ccaaaccgtg
    10801 aggtgatcgt cagacccttg atgggagtta agttggcgaa tagcgtacca cgtacactta
    10861 tcccctgatt ctcaacatct tccttatctc tctgtacgaa cgggtgaccc gagtcagtct
    10921 cggcgtacct tgaaacggca taaaatccgt tttcatcacc caggatgttg tcaccggcgt
    10981 catagtgatt cttcaaatcc tgggtaaggt cattataagt aggaaaatac acaggtgtta
    11041 gaggatcctg tgtcagtgtt cccatcatga aggatccata ctcagtgtgc tggctgaccg
    11101 atctggtttt ccacttttca atagaagtgt ttgtcccgac tgtaagccaa tccttgattt
    11161 tataatcagc gttgatctgg gctgaaagac gttcataaag atccttgtct ccctttacta
    11221 taccgtcatc actgacatag ctgagtgcag tataaaactg gccccggtca tttcctccca
    11281 taaagccaac ggtgtgacgt ttggtcagac tggtagtaaa cacctcttta gcccaattgg
    11341 tatctgtacc gtcataccca agctcattca gacgttgttg tgtaagaaca ccttcagcaa
    11401 ttttatagtc aatatactgc tctgcattca ttacttcagg cgccttgccc agtgaattcc
    11461 atacattctg gaaattatag aagacctgac cgccacccat ttttctgccg gtcttggtag
    11521 tgatcagcac cactccgtta cccgcctggg caccataaat agcagctgat gccgcatcct
    11581 tcagaacctc catggattcg atcatctggg ggtccagata ctgaatgttg tctacctgaa
    11641 gaccatcaac aataagcagt ggccccaggc ctgaggatga attggaagaa tacccgcgga
    11701 cccggatttg tgagcctgcg ccgggagctg ctgaataatt caggatctga acacctgctg
    11761 cctttccttg taaagcactg gctgcatcgg aagtagtacg gtttgcaagg tcaacttccc
    11821 tgacagatga tacggctccg gtaacatcgc tttttctttg gacaccatac ccgaccacca
    11881 cgacttcctc aaggagttcc atatcctcga ccatcgctat ttcaaaagtt cgttggtcac
    11941 ctatggggat cacctgcgat acataaccaa tgaatgagat cctcaaattc gctccggtcg
    12001 gaacttgcag cgaaaattga ccatccaggt cggtaaccgt ataaatacgg tcattgccga
    12061 caacaaccac tgaggctcca ataattggat taccttcagc atcgatgact ttaccgctaa
    12121 ttgctcgagt ctgggcatag gagcctatac tcgtaacaca tgcggctaaa atcaatgcga
    12181 ttttgactac atttagcaat tttttaatca taattagtta ataggttaaa tgaaattagg
    12241 taaaacagtt ttgattttaa aattactaat ctgccgagga accaaatttc aaaatggatt
    12301 catattggtt tgttaatcag tcattaggta ccaagtgacg ctaatatcca ttgagatgaa
    12361 tcaaaactca aagtatatga acgataatag aatcaatggg tgcaatttgc ctccaagacc
    12421 ctttttttct cattcctgaa tcttcggatt accgctttaa cattgttgaa caagaaagta
    12481 cagcacttaa caagacccaa tattgcaagt atttgaacga tttattacat gagtttgagt
    12541 atcttcgtaa cattgaattt ttggtcattt aatatatgaa acagttcata ctgacggttt
    12601 gccttctggc tgccgtatgc ctaaaagcaa gtggtatcga gcaatattac ttcaaacact
    12661 acaagagtga agatggtctg ccgaataata cggtcacttg ctccatccag gatcatttcg
    12721 gattcctctg gtttggtacc aaggatggaa tctgtcgttt caatggcaat gatttcactg
    12781 ttattgacga tcctgatctc agaagcagta ttaactcgat gactcttgcc ctgtgtgaag
    12841 atcatgatgg cttgctatgg tattattcga ccaacgggac cggtttctat gatccataca
    12901 ccgataagat caccagtgtg aaagagcttg acgggcaccg tttttttgat atcgatgtgg
    12961 acaaggacaa caacgtatgg ttcatctcat ccaattcaat cttcaagttg gacagaagta
    13021 caggttccta tgtacggtat tcctctgaaa gaaattttac cgcattgcac tcatgcattg
    13081 ataaagaagg gaatatatgg ttcacaacct atgagggaga cgttcgtagg tacaatcccc
    13141 gtaccgatta ttttgaggta tttccggtga tcactgaaaa aacaatgact tccgacacat
    13201 accccgtata cattgaggcg ataaacgata caaaactatt gatatcaacc aaccattatg
    13261 accttatcag actggatatc gatagcggga actgtgacta tctgacc
//
LOCUS       DAOSOF010000047        12846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4866_length_12846_cov_5.269017, whole genome shotgun sequence.
ACCESSION   DAOSOF010000047 DAOSOF010000000
VERSION     DAOSOF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_12846_cov_5.269017"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLM68_04050"
     CDS             <1..858
                     /locus_tag="PLM68_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT54570.1"
                     /translation="TLLSVLIAGESGTGKEYVANHIHQNSSRRDGPFVALDCGAIPRD
                     LASSELFGHLKGSFTSAISDKKGAFEMADGGTLFLDEVGNLSHDVQMQLLRALQEGVI
                     KPVGSSRIIEVNIRIITATNVDLKQMIEQGKFREDLYHRINEFQLHIPRLQERGQDVL
                     LFARHFLEYANRQMNKQIKGFSPGAEQLMMAYSWRGNLRELLNVVKRTVLLSEGPEIL
                     ATDLPEEIRGLQSVREGQDAHTLKEQHERQMITEALLRTKNNRTRAARLLGIDRKTLY
                     NKMKLYNLE"
     gene            complement(823..>3172)
                     /locus_tag="PLM68_04055"
     CDS             complement(823..>3172)
                     /locus_tag="PLM68_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54571.1"
                     /translation="TISLLYEAEWLGTRFIQDPKAENYDLYRFALDKVDVMLDSLGRG
                     TTMERQQEILQEIDSLLVYRNINIQDIARQQKELSQRSNRDVAQKVDRALPQVPAMTQ
                     TPKLAEITSRPMPPVVLREEVIYDTITTAVHRPRQNFFERLGNAFAPSRMQDSITEIK
                     QIRRTITDSLVRIIPGTPQPVAVQTTDRDTVVQAVMRVLDDVEAARQRQLRAISIQLE
                     QLIETDRALNLQINKLLEELNQEWFSTTLTALETRRASLEKAGSSLSILGVAALLIIL
                     LSTLLIFTDLNKSRKYRRDLEVARSRAEELMKSREKLLLTVTHDIKAPLSAIIGYLDL
                     LKHPSDKGPEQQLKEYLDPMIHSAGHVMELLANLLEYYRLDAHKTQLNPIVTPVRKLF
                     EEAIDVFAPTAGKKGIILNLRTAIPPETCIITDPLRLRQVVMNLLSNAVKFTDKGHID
                     LHVSLDGPELFFSVYDTGKGIAPEAQKRIFDEFTREEASRTPEKEGVGLGLSIVKRTI
                     ELFKGNITLSRNEDSGSTFCVTIPIETTSMQAPDIPLEVKPVYLEPLRVPLRVLVVDD
                     DPAQLALSTEMLRLSGHLVTTAQGAEAAINIVKHVPVDVILTDIQMPNGDGFHLLEQI
                     RCLADTPVIAVTANSYYTSRHFLDKGFSGHLAKPFVKEQLTQALAPITSAVPYELFSL
                     VEMGRMVEADLDAVTQVLRVFHAATSESLKIMEKSLEQGDYGRIASVAHKMLPMFRQL
                     QSPMVKDLIVLERSGKEDPARVSRVIKQAAELLQVIEFHFIV"
     assembly_gap    3173..3182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3716..5185)
                     /gene="cysS"
                     /locus_tag="PLM68_04060"
     CDS             complement(3716..5185)
                     /gene="cysS"
                     /locus_tag="PLM68_04060"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT54572.1"
                     /translation="MPRDLFIYNTLTREKEKFEPLVPGHVGLYVCGPTVYGDPHLGHA
                     RPGVVYDVFVRLLKELGYKVRYVRNITDVGHLENDSDSGEDKIARKARLEELEPMEVV
                     QYYTIRYHQAMALLNTLPPSIEPRASGHITEQIAVIAQILDAGMAYLVNGSVYFDVEK
                     YNKTHRFGILSRRVLEDLMANTRKLEGQEEKRFPFDFALWKKASPQHIMRWPSPWSEG
                     FPGWHLECSVMSSKYLGQVFDIHGGGMDLLFPHHECELAQNTITRGKDSVRYWMHNNL
                     ITINGQKMGKSLGNFITLQELFQGTHPLLTQSFHPMTVRFFILQAHYRGTLDFSNDAL
                     LAAGKGLKRLLQARRVLAKLSVSPEAAVSATLQEMEEKIYQALCDDMNTPIALGTLFE
                     LVTWIHTASDSARPVSQGDKELVERIYSRVVEGILGLREEGDNDRLPLIEGLIDMILE
                     FRTQARATKNWNESDRIRDRLSSLGVHIKDSREGTGWEL"
     gene            complement(5223..5579)
                     /gene="ssb"
                     /locus_tag="PLM68_04065"
     CDS             complement(5223..5579)
                     /gene="ssb"
                     /locus_tag="PLM68_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT54573.1"
                     /translation="MEQSINKVELMGRVGADPRIGQSEEGRRVMNFNLATGEVIKTGT
                     GALKEETTWHRVVAWEGKDIAPFESVKKGTRLIITGKIRTNAYEKDGQPRYIQEVVAQ
                     TLREVDSRKPGDMNPS"
     gene            complement(5653..7734)
                     /locus_tag="PLM68_04070"
     CDS             complement(5653..7734)
                     /locus_tag="PLM68_04070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT54574.1"
                     /translation="MNRTILLFMMFTASLALSQCSGNEHNKAPLIGKNMEIPESDLLN
                     STILNLFGRVSDPQVSPDGKRIVYGVTYTSIKENRRNRELFVMDIDGQHNVQITRTPA
                     SESNARWLDNDRLLFVCDGKLWIMQADGTGRQQVTGTDKSISEFSISPTGDRILFIST
                     VPSGMKPTDLDPSLDKSTGRVIDDLMYRHWDCFVEEIPHSFTALFDGKTLSNVTDLLE
                     GQLFELPTLPFGDIGELCWSPDGKSIAYSCRKMTGKDYAVSTNTDIYLRDLESGQETN
                     LSQGMMGYDTAPRFSPCGNFLVWNSMERDGYEADKNRLMLYSFADGSIKDLTASFRYN
                     MESVTWAPDSKMLYFNSCAQGINQLFSLDPATGKIHQITTGWYDCGPVQISADTGTGA
                     RLIGTYVSMSMPTEVVSVHPETGEYTQLTTENSDIFNHLTMGQVEERWVTTTDNKKML
                     TWVVYPPHFDPQKKYPAVLFCAGGPQSTLSQGWSYRWSHQLIAANGYIVILPNRRGTT
                     AFGQEWTEQISGDYTGQNMQDYLSAVDHMKREPFVDGSRIAAVGASYGGYSVFHLAGT
                     HNERFAAFIAHAGIFNTEHMYMTTEEIWFPHWDNGGAPWDKNPVAVNHYAQSPHKRVQ
                     YWNKPILITHGEMDYRIPVDQAMAAFNAAKLHGVPARLVLFPDENHWILKPQNNMLWN
                     KVFFEFLEKYM"
     gene            7803..8702
                     /locus_tag="PLM68_04075"
     CDS             7803..8702
                     /locus_tag="PLM68_04075"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOT54575.1"
                     /translation="MKRLVSLLIVFAALSMQAQSIKMEFKPEKGVEYPVFLEINQTMT
                     LIVPGMGNVVTQTDQIIGAVVTLAEEVEQGYLVEARVTQMTIKAQGQGQSQVFDSEGE
                     DVLGQAIRSMMGQPLQMIISRRGEILEQMPVEDTIYSQADSILATQYARRRREQTMVQ
                     IKQMFSQNTIKSVVQAGLTKFPEREMIIPSVWTDEEPSQELGAIVTVQQRLIEVTGNR
                     AIITAKSVIMPDPNAKKPDTPQRMEGISGNGEATSVIDIQSGWVVESNGTQSLRGELV
                     VEQGGQVQAIDLTMEVKTKITGK"
     gene            complement(8689..9219)
                     /locus_tag="PLM68_04080"
     CDS             complement(8689..9219)
                     /locus_tag="PLM68_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOT54576.1"
                     /translation="MRTLKFIFLTAILTGIFHMSAAQNPSSFATSIDGEASITLGIDL
                     RIEKLTDLHFGNIMSQSSGGSVTLNPSTGNVTASSGSFQFMGTSTAATFMLTGLPGQS
                     FLISSGSYPTQITLKNENNYTMTVNNFQFDPSSGQIGNDGTAILKLGGTLNIAANQPG
                     GNYSNTSDLTVTITYQ"
     gene            complement(9294..>9960)
                     /locus_tag="PLM68_04085"
     CDS             complement(9294..>9960)
                     /locus_tag="PLM68_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54577.1"
                     /translation="LEEREELAYAEILAGNVPEYMKGFVRITTRQKDLNGKTHHVTLF
                     VKPDYLTVLSGNVPFIVPLTPMIAGRIAESLDCSLPTPKIVDIIFHHAEATAEPFNYI
                     PREYRNLDPDLFFDHSQVIFAQLRAAGKKPGMLTAGTKKDVVITRVPPNLSRTRHVII
                     YGWHRPDGTPIQPVYDGHINTYVDYSHGVRLISNKVLVDGKEYHYQELLRDPILWPLI
                     SGD"
     assembly_gap    9961..9970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10192..10437
                     /locus_tag="PLM68_04090"
     CDS             10192..10437
                     /locus_tag="PLM68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT54578.1"
                     /translation="MAVNIEEKVKQIIVDKLGADEKEVTPTANFTNDLGADSLDTVEL
                     IMEFEEAFGINIPDEAAEKISTVGDAIKYIKDNVKAE"
     gene            10446..11702
                     /gene="fabF"
                     /locus_tag="PLM68_04095"
     CDS             10446..11702
                     /gene="fabF"
                     /locus_tag="PLM68_04095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT54579.1"
                     /translation="MKLKRVVITGLGTINSLGHNVPEYFDNLVAGKSGSGWITRFDAS
                     LFKTRFACEIKNYDPEKFGIDKKDAHRNDPFTQYAMIAAHEALMDSRLDLDALDKDRV
                     GVIVGSGIGGIETLLKEIGEYYKGGMVPRFSPFLVPMMIADIASGMISIQYGFRGPNY
                     ATVSACSTSNHAMSDAFTLIRMGKANVVVTGGSEAPINEASVGGFNAARALSTRNEEY
                     LTASRPFDRTRDGFVMGEGAGVLVFEEYEHAKARGAKIYAEVGGAGLTADAYHITAPH
                     PDGGGARRVMAEAISEAGLTPQDVDYINLHGTSTPLGDVVEVKAIKDLFGDHAYKMNL
                     SSTKSMAGHALGATGAIEILACICAMERDMIPPTINFKEEDPEIDYKLNFTFNQAQRR
                     KVRVALSNTFGFGGHNSSLLIKKVEE"
     gene            11699..12406
                     /gene="rnc"
                     /locus_tag="PLM68_04100"
     CDS             11699..12406
                     /gene="rnc"
                     /locus_tag="PLM68_04100"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT54580.1"
                     /translation="MIFRHADKNPKDKTLRKVLRDCFGFQPKNLDIYKIALTHRSAVV
                     AYLESNERLEFLGDAVLDMVVGEAVYLRYPNGDEGYLTQIRSKVVSRDSLNKIAEQTG
                     LLPFMEVKGNDNQSRNIGGNMLEALAGAVFLDVGFTKCRKVLQHLLLSHVNWQELECT
                     ETDYKSRIVERCQKKGKTIGFRVHMLPQTPGNPSLFEAEAISDGTVLGTGRGTSKKDA
                     EQEACRQAWLREFNRGH"
     gene            complement(12403..12795)
                     /locus_tag="PLM68_04105"
     CDS             complement(12403..12795)
                     /locus_tag="PLM68_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT54581.1"
                     /translation="MQRLFLSLLFLMSVMTLRAQVYDLEPGIFLQMIEANPHAQVLDL
                     RDPLEFEYRHIPGAVNIQPASVYFLEEVRNQLSASDTLYIYCRMGKSTKEVTQLLLDN
                     GYRVIFNLRGGSVAWDAHLDKERRRKKR"
BASE COUNT         3168 a         3057 c         3365 g         3236 t
ORIGIN      
        1 acgcttcttt ctgtgctcat cgccggggaa agcggcacgg gaaaggaata tgtggccaat
       61 cacatacacc agaacagttc ccggcgggac gggccttttg tggccctgga ctgcggtgcc
      121 attcccaggg atctggcctc gagcgagctg ttcgggcacc tgaaagggtc ttttacctcg
      181 gccatcagtg ataaaaaagg agcttttgaa atggcagacg ggggaacgct gttcctggac
      241 gaggtgggga acctctcgca cgatgtacaa atgcagctgc tacgggcgct gcaggagggg
      301 gtgatcaagc cggtgggtag ttcccgtatc attgaggtca acatccggat catcaccgcc
      361 accaatgtgg atctgaagca aatgatagaa cagggaaaat tccgggaaga cctgtaccac
      421 cgcatcaatg aattccagct gcacattccg cggctgcagg aacggggaca ggatgtgctg
      481 ttatttgcca ggcatttcct ggaatacgcc aaccgtcaga tgaacaagca gatcaaggga
      541 ttttcaccgg gggcagaaca attgatgatg gcttacagct ggaggggaaa cctgcgggag
      601 ttgctcaatg tggtaaaacg taccgtactc ctgagcgaag gcccagaaat cctggccaca
      661 gacctgcccg aagaaatacg gggacttcag tctgtgcgcg aaggtcagga tgcccatacg
      721 ctgaaagaac agcatgaacg gcaaatgatc acggaagcac ttctccgaac caagaacaac
      781 cgtacacggg cagcacgttt gctggggatt gaccgcaaga ccttatacaa taaaatgaaa
      841 ctctataacc tggagtaact ctgcggcctg tttgatcaca cggctcaccc ttgcgggatc
      901 ttctttcccg gaacgttcca gaacaataag gtccttgacc atgggcgatt gcaattgccg
      961 aaacatgggg agcattttat gagcaacaga ggctatcctt ccataatcgc cctgttccag
     1021 agacttttcc attattttaa ggctttcgct tgtggcagca tgaaaaacac gcaacacctg
     1081 cgttacagca tccagatcgg cttccaccat gcgtcccatt tccaccagtg aaaacagctc
     1141 gtagggaact gcagatgtta tgggtgcaag cgcctgggtc agctgttctt tcacaaaagg
     1201 cttagccagg tggcctgaaa accctttatc caaaaaatgc cgggatgtat agtaactgtt
     1261 cgctgtcact gcaattacgg gagtatctgc caggcatctt atctgttcca gtaaatggaa
     1321 tccatctccg ttcggcatct ggatatccgt caggatcaca tctaccggta catgttttac
     1381 tatgttgata gcagcttctg ctccctgcgc cgtggtaacc aggtggcccg aaagacgcag
     1441 catttccgtg gaaagggcta attgagccgg atcatcgtcc acaaccagga cgcgcagggg
     1501 aacgcggagc ggttccaaat aaaccggttt gacttcaagg ggaatgtccg gtgcctgcat
     1561 gctggttgtt tctattggta tggtaacgca gaaagtactc cccgaatcct cgttcctgga
     1621 tagggtaata ttgcctttga acagctcaat agtacgtttt actatggaaa gacccagtcc
     1681 aacgccttcc ttttcaggag tgcgggacgc ttcctcgcgt gtgaattcat caaagatgcg
     1741 cttttgggcc tccggggcaa tgcccttccc ggtgtcatat acagaaaaga ataattccgg
     1801 accatccagc gaaacgtgca ggtctatatg tcccttgtct gtaaatttaa cagcgttgga
     1861 aagcaggttc atcacaactt gccgcaaacg caaagggtct gtaattatgc atgtttccgg
     1921 gggaatggcc gtgcgcaggt tcaggataat cccctttttg cctgctgtcg gggcaaacac
     1981 gtcaattgct tcttcgaata atttcctgac aggtgtcacg atgggattga gctgcgtttt
     2041 atgcgcatcc aggcggtagt attccagcag attggccaac agctccatca cgtgtccggc
     2101 agagtgtatc atggggtcca ggtattcttt caattgttgt tcggggccct tgtccgaggg
     2161 gtgcttaagc aggtccaggt accctatgat ggcgctcagg ggagctttga tgtcgtgcgt
     2221 tacggtaagc agcagttttt cccggctttt catcaattcc tcggcacggc tgcgggccac
     2281 ttccagatcc cgccggtatt tccggctttt attcaggtca gtaaaaataa gcagggtgga
     2341 gagcagaatg atcagcaaag cggctacgcc caatatggac agtgagctcc cggccttttc
     2401 cagcgaagcc ctgcgggttt ccagggccgt aagagtagtg ctgaaccact cctggttcaa
     2461 ttcttccaat aatttattga tttgcaggtt cagggcccgg tctgtttcta tcagctgctc
     2521 cagttgtatc gagatggccc taagttgacg ctgccgtgcg gcttccacat cgtccagaac
     2581 cctcatgacc gcctggacca cggtgtcacg gtctgtggtc tgaacggcta caggttgcgg
     2641 ggtccctggt atgatgcgga ccagggaatc ggttatggta cggcggatct gctttatctc
     2701 ggttatgctg tcctgcatgc gggaaggagc aaaagcgttg cccaaccgtt caaagaaatt
     2761 ctgcctgggc cggtgtaccg ccgttgtgat ggtatcgtaa atgacttctt cacgtaagac
     2821 aacaggtggc atgggacggg aagttatctc ggccagtttt ggggtctgtg tcatagccgg
     2881 aacctggggt agcgccctgt ccactttttg cgccacatcc cggttgctgc gctgggagag
     2941 ttccttctgc tgccgggcaa tatcctgtat gtttatgtta cggtatacca gcaggctgtc
     3001 aatttcctgc aggatctcct gttgacgctc catggtggta ccccttccca gggaatcgag
     3061 catcacatct accttgtcca gggcaaagcg gtagaggtca taattttcgg cttttgggtc
     3121 ctggataaaa cgagttccca gccattcggc ttcgtataac agtgatatgg tgnnnnnnnn
     3181 nngtgttgct gatcaaaagg cttcgttcca ggatctcttc ccggcggtca tcgctctgaa
     3241 agagaaacgg gtacacacgt ttgtacacgt accagccagc accggcgatc agagccgcca
     3301 acaatatgta actgaaaacc actttcaggc gaaaacccac atcttgtttc atgaagctaa
     3361 gatataaaga aatcccagta gcacaaaagg gggaaaatga actgtttatt tggggaatta
     3421 ttccccaaat ggcacactca gttccccaac atatttctca ggtcgttggc tttctttttt
     3481 gtcagtgaaa aaacagttct gaaaatcagc agattgaaaa tcggggaaag tttttggaac
     3541 gctatttgac tatattattg acaactaaag cagtgagcac gcggaatttt ttcatgcaaa
     3601 cagaagatcc atgggtcgtg ccacgaaata aagagggata ctgaaaggcg acagtatccc
     3661 tctttacatt tcctgctttc acggataggc ctgcttttcc tggccgaata cgtgcttaca
     3721 actcccagcc ggttccttcc ctggagtctt ttatatgaac gcccagcgag gaaagccggt
     3781 cgcggatgcg gtccgattcg ttccagttct tcgtggccct ggcctgagtc cggaattcca
     3841 ggatcatatc tatcagtcct tcaatcaggg gaagccggtc attgtctcct tcttccctca
     3901 ggcccaggat tccttctacc acccggctgt atatccgttc cacaagctcc ttgtctccct
     3961 gcgaaacagg gcgggccgaa tcagaggcgg tatgtatcca ggttaccagc tcaaaaagcg
     4021 ttcccagggc aatgggtgtg ttcatatcgt cacataaggc ctggtagatc ttttcctcca
     4081 tttcctgcaa cgtagccgaa acggccgcct ctggagaaac ggatagtttt gcaagcaccc
     4141 tgcgtgcctg gagcagcctt ttaaggcctt tgccggcggc tagcagggca tcgttactga
     4201 aatcgagtgt tccgcgataa tgtgcctgca ggatgaaaaa cctgacagtc atgggatgga
     4261 aggattgagt aaggagaggg tgggtaccct ggaacaattc ctgcagggtg atgaaatttc
     4321 ccagggattt gcccattttc tgtccgttga tggtaatcag gttgttgtgc atccagtacc
     4381 gcacgctgtc tttgcccctt gttatggtat tctgggccag ttcacattca tggtgcggaa
     4441 acagcaggtc cattcccccg ccatggatat caaatacttg tccaaggtat ttggaactca
     4501 tcacagaaca ctccaggtgc cagccgggga aaccctcgct ccatggcgac ggccagcgca
     4561 tgatatgctg cggggatgct tttttccaaa gcgcaaaatc aaagggaaag cgcttttcct
     4621 cctggccttc cagcttacgc gtattggcca tcaggtcttc caggacacgc cttgaaagta
     4681 tcccgaaacg gtgtgtcttg ttgtattttt caacgtcaaa atagacagac ccgttgacca
     4741 ggtaagccat accggcatcc aggatttgcg ctatcacggc tatctgttcg gtaatatgtc
     4801 ccgaggctct tggctctatg ctgggaggca gggtgttgag gagggccatg gcctggtggt
     4861 accggatggt gtaatactgt accacctcca tgggctcgag ttcctccagc cgggctttgc
     4921 gggcgatctt gtcttctccc gaatccgagt cgttttccag gtgtcccaca tctgttatgt
     4981 tgcggacata ccggacttta taacccagct ctttcagaag tctaacaaat acatcataaa
     5041 ccacacccgg acgggcatgt cccaggtggg ggtcgccgta tacagtgggg ccgcagacat
     5101 aaagacccac atgtccggga acaagtggct caaatttttc cttttccctg gtaagggtat
     5161 tgtatatgaa tagatctcgt ggcatgcggg ttggtaatta tgtctgcaaa tgtacgtaaa
     5221 ttttaacttg ggttcatgtc acccggtttc ctggagtcca cttcccggag tgtctgtgct
     5281 acaacttcct ggatataccg gggctggccg tctttttcgt aggcattggt tcgtatcttg
     5341 ccggtgatta tcaggcgggt tccttttttg accgattcaa aaggggcgat atctttcccc
     5401 tcccaggcca cgacgcggtg ccaggttgtc tcctccttga gcgctcccgt gcctgtttta
     5461 atcacctctc cggtagccag gttgaaattc atcacccttc ttccttcttc cgactggccg
     5521 atcctgggat ctgctcccac gcgtcccatc aattctactt tgttgataga ttgttccata
     5581 atgcgttatt attagttaaa cgcatcaaac ctataacagc ggtgcgtaaa aaatgacctg
     5641 tatcgtaaaa acctacatat atttctccag gaactcaaag aaaaccttat tccatagcat
     5701 gttgttctgt ggtttcagta tccagtggtt ctcatcgggg aagaggacca gcctggcagg
     5761 cacaccgtgc agtttggcag cattgaaagc agccatggcc tggtccacgg gtatacggta
     5821 gtccatttct ccatgggtaa tcaggatggg cttgttccag tattgcaccc gcttatgcgg
     5881 ggattgggca tagtggttca cagcaacagg attcttgtcc catggggcac cgccgttgtc
     5941 ccagtgcgga aaccagatct cctccgtggt catgtacatg tgttctgtat tgaaaatgcc
     6001 tgcgtgagca atgaaggcgg caaagcgttc gttatgtgtc cctgccaggt gaaataccga
     6061 atatcctcca taactggctc ccacggcagc tatccggctg ccatcaacaa agggctctcg
     6121 tttcatgtgg tccacagcac tcaggtaatc ctgcatgttc tggcctgtat aatcgccgct
     6181 gatctgttcc gtccattcct gtccaaaagc tgttgttccc cggcggttgg gcaggatgac
     6241 gatatacccg ttggctgcta tcaactggtg gctccagcga taggaccaac cctgggaaag
     6301 ggtggactga ggtcccccgg cacagaagag cacggccggg tatttctttt ggggatcaaa
     6361 gtgtggcgga tataccaccc aggtaagcat tttcttgtta tcagtagtgg taacccaccg
     6421 ttcttccacc tggcccattg tcaagtggtt gaatatgtca ctgttttcag tggtcagttg
     6481 ggtgtattct ccggtttcgg gatggacgga gaccacctcg gtgggcatgg acatggaaac
     6541 ataggtgccg atgagccgtg ctccggttcc cgtgtcagcc gaaatctgaa ccggtccgca
     6601 atcataccag ccggtagtga tttgatgtat cttgcccgtt gccgggtcca ggctgaacaa
     6661 ctggtttatt ccctgggcac aggaattgaa atacaacatt ttgctgtctg gggcccaggt
     6721 gacactttcc atattgtaac ggaatgatgc cgtaaggtct ttgatggagc cgtccgcgaa
     6781 tgaatacagc ataagccggt ttttgtcggc ctcgtagccg tcccgctcca tgctgttcca
     6841 caccaggaag ttgccgcatg gtgaaaatct gggggctgtg tcgtaaccca tcatgccctg
     6901 gctcaggttg gtttcctgtc ctgattccag gtccctcagg tagatgtctg tattcgtgga
     6961 tacggcgtag tctttgcctg tcattttgcg acaggaatag gcaatacttt tcccatccgg
     7021 gctccagcac agttccccta tatccccgaa gggtagcgta ggcagttcaa aaagctgtcc
     7081 ttccagcagg tcggtcacat tggaaagggt ctttccgtca aaaagggctg taaaggaatg
     7141 gggtatctct tctacaaaac aatcccagtg acggtacatc aaatcgtcaa tgacacgtcc
     7201 ggtggactta tccagggagg gatcaagatc ggtgggcttc atgccggagg ggacggtgga
     7261 aataaagagt atcctgtccc cggtaggaga gatgctgaac tcagaaatgg atttgtccgt
     7321 tcctgttact tgttgccgtc cggtcccatc ggcctgcatg atccataatt tgccgtcgca
     7381 gacaaagaga aggcggtcat tgtccagcca gcgggcgttg gattcgcttg ccggtgtccg
     7441 tgttatttgt acgttatgct gcccgtcgat atccattaca aaaagttccc ggttgcgcct
     7501 gttctctttt atagaggtat aagtaacacc atacacgatc cttttaccgt cgggagaaac
     7561 ctggggatcg ctaacgcgtc cgaaaaggtt caggatggtg gaattgagca ggtcactttc
     7621 gggtatttcc atgtttttcc ctatcagggg ggccttgtta tgctcgttgc cagagcattg
     7681 agacagagcc agagaggctg taaacatcat aaatagcaag attgttctgt tcataaggtt
     7741 gtggtttgac ttcaaaatta gtgaattaat tgtaagtttg tggaaattta aagaaaagag
     7801 caatgaaacg acttgtaagc ttgttaattg tgtttgccgc cctttccatg caggcacaaa
     7861 gcattaaaat ggaattcaaa ccggaaaaag gggtggaata cccggtgttc ttggagatca
     7921 accagactat gaccctgatt gttcccggta tggggaacgt tgtcacacag acagaccaga
     7981 tcattggagc tgttgtgacc ctggcagaag aggtggagca gggatacctg gtggaagcac
     8041 gtgtgacaca aatgaccata aaagcccagg ggcagggaca atcgcaggtt tttgattcgg
     8101 aaggggagga cgtcctgggg caggccatca gaagtatgat gggtcaacca ttgcaaatga
     8161 tcatttcccg caggggggag atcctggagc aaatgcccgt ggaagatacc atttatagcc
     8221 aagccgacag catcctggct acacaatatg cccgccgtcg cagggaacaa accatggtac
     8281 agataaaaca gatgttcagc cagaatacca taaaaagtgt ggtacaggca ggtctgacca
     8341 agttcccgga acgggaaatg atcattcctt cagtctggac agatgaggag cccagccagg
     8401 agctgggtgc cattgtcacg gtacagcagc gtctgattga agtaacgggc aacagggcca
     8461 tcattacagc aaaatcggtc atcatgcccg atcccaatgc caagaaacct gacacaccgc
     8521 agcgcatgga ggggatatcg ggaaatggcg aagccacttc ggtaatcgat atacagagcg
     8581 gatgggtggt tgaaagcaac ggaacacagt cgctgcgggg tgagctggtg gttgaacagg
     8641 gaggacaggt tcaagcgata gatctgacca tggaagtgaa gaccaaaatt actggtaagt
     8701 gatcgttacg gtcagatccg aggtgttgga ataattcccg cccggttggt tggctgcaat
     8761 gttgagggtt cctccaagct tgaggatggc ggtaccatca ttgccgattt gtccgcttga
     8821 gggatcgaac tggaaattat tcacagtcat tgtgtagttg ttttcgtttt ttagggtgat
     8881 ctgggtgggg taactgccac tggatatcag aaaactctgg cccggcagtc cggtaagcat
     8941 aaaggttgcg gcagtactgg tacccatgaa ctgaaaagag cctgaagaag ccgtaacatt
     9001 cccggttgac ggattaagcg tcacagatcc tccggaggat tgactcataa tgttcccgaa
     9061 gtgcaggtcg gtcaattttt ctatccgcag atcaatcccc agcgtgatgg aagcttctcc
     9121 gtctatggaa gtggcgaatg aactcggatt ctgtgccgcg gacatgtgaa aaatcccggt
     9181 cagaattgca gtcaggaata tgaatttaag tgttctcata tgagcggaat cctcttataa
     9241 aatcaaatat agggaaaatt ctgcaaaaat caaaccacat tcaaacaaca agttcaatca
     9301 ccgcttataa gcggccacag tattggatcc cggagcaact cctggtaatg gtattctttt
     9361 ccgtctacaa ggaccttgtt tgaaatcaag cggactccgt ggctgtaatc aacatatgtg
     9421 ttgatatgac cgtcgtaaac aggttgtatg ggtgtgccat cgggcctgtg ccatccatag
     9481 attatgacat ggcgtgtacg ggacaggttg ggtgggaccc tggtgatcac aacgtctttt
     9541 tttgttccgg ctgtaagcat tccgggtttt ttgccggcgg ccctgagctg ggcgaagatc
     9601 acctgtgaat ggtcaaagaa caggtctgga tccaggtttc ggtattcgcg cggaatataa
     9661 ttgaaaggtt ctgctgttgc ctctgcatga tgaaagataa tatccacgat ttttggcgta
     9721 ggcagcgaac aatccagtga ttccgcaatc cggcctgcga tcatgggtgt caacggtaca
     9781 ataaaaggta cattgccgct cagtactgta agataatcgg gctttacaaa tagggtgaca
     9841 tggtgcgtct tcccgttcag gtctttttgc cgggtggtta tgcggacgaa tcctttcatg
     9901 tattccggca cgttcccggc cagtatttca gcatatgcaa gctcttcacg ctcttccagg
     9961 nnnnnnnnnn agaaaggaca atctccccgc cggacctggg ccctcaccat acaggcgggt
    10021 agcagcataa gtgttgccag ataaaggaaa gctgaatttc gcaggctgtg tttcatgggt
    10081 caggaacgtc cggaatttcg ttaattggaa ttattttttt tactttgtca aatgcaaagt
    10141 tcgcgcaatt ttgcattatt aactacattt ttattaacta ataaacaaat tatggcagtt
    10201 aacattgaag aaaaagttaa gcaaatcatt gtggataaac tgggtgcaga tgagaaagag
    10261 gtaaccccca ctgctaactt tacaaatgat ttgggtgcgg attctttgga taccgtagaa
    10321 ctgattatgg aatttgaaga agctttcggc atcaatattc cagatgaggc agctgaaaag
    10381 atatcaacag taggcgatgc catcaaatac ataaaagaca acgttaaagc cgaataatta
    10441 aaaccatgaa gctcaaacgg gtagtcataa caggattagg aaccataaat tccctgggac
    10501 ataatgtccc tgaatacttt gacaatcttg tcgccggcaa gtccggttca ggatggatta
    10561 cccgttttga cgcttctttg ttcaaaaccc gttttgcctg tgagatcaag aactatgatc
    10621 cggagaaatt cggcatagac aagaaggatg cccatagaaa cgatcctttt acacaatatg
    10681 ccatgatcgc tgcccatgaa gctttgatgg acagccgtct ggacctggat gccctggata
    10741 aggaccgggt tggagtgata gtgggatcgg gcataggcgg cattgaaaca ctgcttaaag
    10801 agatcggaga gtattataag ggcggcatgg ttcccaggtt cagtcccttt cttgttccga
    10861 tgatgatagc agacatagca tcagggatga tctctataca atacggattc cgtggaccca
    10921 attatgccac ggtatctgcc tgttccactt caaatcatgc gatgagcgat gcctttaccc
    10981 tgatccgtat gggaaaggca aatgttgtag taaccggggg ctctgaagct cccataaacg
    11041 aggcaagtgt tggcggtttc aatgccgcca gggcgctttc caccaggaat gaagaatacc
    11101 tgactgcttc acgtcctttt gacaggaccc gggacgggtt cgtgatgggg gaaggagccg
    11161 gtgtccttgt ttttgaagaa tacgaacatg ccaaagcccg aggggctaaa atatatgccg
    11221 aggtgggggg tgccggcctg accgccgatg cttaccacat cacggcacct catcccgacg
    11281 gggggggcgc ccgccgtgtc atggccgaag ccatcagcga agcaggatta acaccacaag
    11341 acgtggatta tatcaatttg cacggaactt ccaccccttt gggagatgta gtggaagtaa
    11401 aggccataaa agatcttttc ggcgatcatg cttacaaaat gaacctgagt tccaccaaat
    11461 ctatggcagg acatgccctg ggcgctaccg gcgccattga aatacttgcc tgtatctgtg
    11521 ccatggaaag ggatatgatt cctccgacca tcaatttcaa agaagaagac cccgaaatcg
    11581 actacaaatt gaatttcacg ttcaaccagg cccaaaggag gaaagtaagg gtagctcttt
    11641 ccaacacttt tggcttcgga ggacataact ccagtctgct aatcaaaaag gtcgaagaat
    11701 gattttccgc catgcggata agaaccctaa agacaaaaca ctacggaaag tcttgcgtga
    11761 ttgttttggt tttcaaccaa aaaatcttga tatctacaaa atagcactga cacaccggtc
    11821 tgcggttgtt gcgtatttag aatccaatga gagactggaa tttctgggtg acgccgtatt
    11881 ggatatggtg gtgggtgaag ctgtttacct gcgttatccc aatggtgacg aggggtacct
    11941 gacgcagatt cgctccaagg ttgtgagccg cgattccctg aacaagatag cagaacagac
    12001 aggactatta cccttcatgg aggtcaaggg aaatgataac caatcccgca acataggggg
    12061 taatatgctt gaggcacttg caggagctgt ctttcttgat gtggggttta caaaatgcag
    12121 gaaagtcctg cagcatcttt tattgtccca tgtcaactgg caggagctgg agtgtacaga
    12181 aacagattat aaatcccgca ttgtggagcg ctgtcagaaa aaagggaaaa caatcgggtt
    12241 ccgtgttcat atgttgcctc agacaccggg caacccgtct ctgtttgaag ccgaagccat
    12301 ttcagatggc actgttctgg gtaccggccg gggaacttcg aaaaaagatg cagaacagga
    12361 ggcctgccgg caggcctggc tgcgtgaatt caacaggggc cactaacgtt tcttccggcg
    12421 tctttctttg tcaaggtgag catcccaggc aacgctcccc ccacggagat tgaatattac
    12481 ccgatacccg ttgtccagga gcagttgcgt aacttctttt gttgacttgc ccatgcggca
    12541 atatatatat aaagtatcgg aagcggataa ttggttgcgg acttcttcca ggaaatacac
    12601 gcttgcaggc tggatgttga ccgcaccggg aatatgccgg tattcaaact caagagggtc
    12661 acgcagatcc agcacttgtg cgtggggatt tgcttcgatc atttgtaaaa aaatcccggg
    12721 ttcaagatca tatacctgtg ccctgagagt catcacggac atcaggaaca ggagtgacag
    12781 gaataagcgt tgcatgttgt gacctcggag ggattcgaac cctcgaccca atgattaaga
    12841 gtcatt
//
LOCUS       DAOSOF010000048        12823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4879_length_12823_cov_5.045426, whole genome shotgun sequence.
ACCESSION   DAOSOF010000048 DAOSOF010000000
VERSION     DAOSOF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_12823_cov_5.045426"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..877
                     /locus_tag="PLM68_04110"
     CDS             272..877
                     /locus_tag="PLM68_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOT54582.1"
                     /translation="MKQFEINATKRGNFTKNAVKQLRRDGMVPCVLYGNKTENIHFSV
                     LEKDLKDLIYTPSSYIISLNIEGKKSLCILHEVQFHPVTERVLHLDFLAIDKSRPISI
                     TIPVVISGNSEGVRQGGKMQVLNRKLLVNGLLKDLPDQLDIDVTDLTLGKSITAGDLK
                     FDNIRITSPRSTIICMVKMTRASTSASAATEEETQEIPAAE"
     gene            893..1468
                     /gene="pth"
                     /locus_tag="PLM68_04115"
     CDS             893..1468
                     /gene="pth"
                     /locus_tag="PLM68_04115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT54583.1"
                     /translation="MSFLVTGLGNIGTEYAGTRHNIGFSVLDAWAALHKTSFSTLRYG
                     SLACIKHAGKQVWLLKPSTYVNLSGKAVRYWMKEESVPMENLMIVLDDVALPFGKLRM
                     RAKGSDGGHNGLKNISLLLETEGYARLRIGVGGDFSRGYQADYVLSHWNEQEARELPF
                     ICNNAIQALELYIRQGIDRAMNLVNTTDSGN"
     gene            1464..1844
                     /locus_tag="PLM68_04120"
     CDS             1464..1844
                     /locus_tag="PLM68_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOT54584.1"
                     /translation="MELMRIDKYLWAIRVFKTRAESVEACRGSRVKINDQPVKPSREV
                     RQGDTIVVRKGAVFYQYLVLQPVERRQPARDVPLYARDVTPQEELDKLQMPRETVIIY
                     RKKGTGRPTKKERRQLDDIMDING"
     gene            complement(1834..2739)
                     /locus_tag="PLM68_04125"
     CDS             complement(1834..2739)
                     /locus_tag="PLM68_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOT54585.1"
                     /translation="MNNTKLYPLSFKPILKERVWGSETWVLSGLDSDTSIINNGFLKG
                     NTLNEALEVYLTDLVGDRIYNMCGDEFPLLIKYLTVNDFLSVQVHPDDERAAGMHHAY
                     GKTEMWYIMDADPKARLYLGFNQDMSPESFYSRCENETLPDVMNCIVPKKGECYFIPA
                     GLIHACGGGLTIAEVQQVSDITYRVYDWGREHNRETARGMHLELALDAIDYKKHVPNP
                     TTSGVLADSPHFTVRLLEMDKQTEIDSSAADNFVVYLELEGSLGIETEGMAYRSANGC
                     LLIPASVDYWKIIPEGKARFLEITR"
     gene            complement(2705..3604)
                     /locus_tag="PLM68_04130"
     CDS             complement(2705..3604)
                     /locus_tag="PLM68_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT54586.1"
                     /translation="MRKYLDHYRPGLGESWLIMLAIFLVAGTIITVSVVFIINQIFPG
                     TLTLTGASDWSTPIVYVLPFTFVFLYVYLRSRNKFREAVLSNRPPYPNPRPALGRVPL
                     PLFILLLPVLIISLSVLLDPLTDLLEMPEFMKEIFERMTESNLPTFVAVVIAAPILEE
                     WLLRGLALKGMLQHMAPWKAIAWSALMFGVIHANPWQAIPAFLIGCLLGWIYYRTRSY
                     WTCVALHAINNGLSFLLAIWFPQLAADASLRSIIGDTPFFITLGASAVVLVISLSYLN
                     KYLAPAPSLIINHEQHETVSPEL"
     gene            complement(3618..4538)
                     /locus_tag="PLM68_04135"
     CDS             complement(3618..4538)
                     /locus_tag="PLM68_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOT54587.1"
                     /translation="MRPTLVIMAAGMGSRYGGLKQLDTLGPNGETIMDYSIYDALQAG
                     FGRIVFIIRESFREEFNRVCAARYGNSFPFAFITQELADIPAPFSPPAEREKPWGTGH
                     ALLTTSRCVNEPFGIINADDFYGRDAFRVLHDFLVRKDLGKGSCAMVGYRLENTLSPS
                     GPVSRGICHSNREGLLTDVVERFKIKRYDNGKIYCTIPGNKQEATLLELQPGDLCSMN
                     CWGFLPDIYPKARRLVTAFLEQHILEPASEYHLPTMVNQIIKSGEGNCHVLQTDASWF
                     GVTYREDRDGVVSRLQSLHREGLYPNPLFL"
     gene            4580..4918
                     /locus_tag="PLM68_04140"
     CDS             4580..4918
                     /locus_tag="PLM68_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT54588.1"
                     /translation="MLKRFKSDRLLKFIRNRYFIVTMVFIVWVLFFDNNNLISWVRTL
                     GQYSANKSQQRYYKEKIQQTENNLRQLQSNKDSLEKFARENYYFHRQDEDVFIVTPGP
                     SDPSDRFPYR"
     gene            complement(4872..5570)
                     /locus_tag="PLM68_04145"
     CDS             complement(4872..5570)
                     /locus_tag="PLM68_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOT54589.1"
                     /translation="MKYRFFLFDLDNTLWDFEGNARECITELIERHGLGAYIPDPAAF
                     YDMYRTSNKQLWEMYEAGEITQQKLRDVRFVITLEQAGVPHAGSLGPVFGEQYLELMP
                     KKTNLVPYAREVLEYLQRKGCKMALLSNGFKQVQYHKVRNSGLDPFFENRIFISEEVG
                     YHKPNPKIFIAAITSVNGKKKETIMVGDNFLTDIEGAQVFGIDQFFYNPDGLPCDGAP
                     TYMGNDLRDLMDLA"
     gene            complement(5571..6914)
                     /gene="purB"
                     /locus_tag="PLM68_04150"
     CDS             complement(5571..6914)
                     /gene="purB"
                     /locus_tag="PLM68_04150"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT54590.1"
                     /translation="MEFNPLLAISPIDGRYYAQTAPLNQYFSEFALIRYRLHVEVEYF
                     IALCKLPLPQLKDVDSRWFPILRNLYKNFSITDAKVIKAQEKVTNHDVKSVEYFLKNK
                     MKALGLDKYYEFVHFGLTSQDINNTAIPMSLKHGIQKEYIPLLRQIIDMLEERSREWA
                     PVVMLAHTHGQPASPTRLGKEIQVFKVRLEEQLKSLEQIPFAGKFGGSTGNFNAHNLA
                     YPQIDWHKFADKFLNSTLGIQRSFPTTQIEHYDYIAALCHNLSRINTICIDLSRDMWS
                     YVSMNYFKQHIKDQEIGSSAMPHKINPIDFENAEGNFGVANAIFCHLAAKLPVSRLQR
                     DLTDSTVLRNLGVPLAHTLLAFKSLQKGFEKVLLNMPAIEKDIDENWAIISEAIQTIL
                     RRENYPQPYETLKVLTRTNMHIDKEYLHAFIRNLDISQEVKDELLQITPKNYIGH"
     gene            complement(6929..7723)
                     /gene="folP"
                     /locus_tag="PLM68_04155"
     CDS             complement(6929..7723)
                     /gene="folP"
                     /locus_tag="PLM68_04155"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT54591.1"
                     /translation="MNICFPAIMGVVNCTPDSFYAGSRVQDTDCALNRIETILREGAA
                     IVDIGACSTRPGASWPSPMEEWNRLEPVLLQAKEKFPEALFSIDTFRPVIVKKAYELL
                     GRFWVNDIFAGCYDNAMMPLIASLGLPWIAMHQERYDGPEGITRFFNRIRDAAARYGI
                     KEYILDPGFGFNKDVDQNYEVLSRLHTLVPKNEKGDPCAPLLAGISRKRMTYIPLNTT
                     PDNALVSTSALHLQLLLKGVSILRVHDVAQAADMIAIYRKMSIFEH"
     gene            7774..8442
                     /gene="tmk"
                     /locus_tag="PLM68_04160"
     CDS             7774..8442
                     /gene="tmk"
                     /locus_tag="PLM68_04160"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT54592.1"
                     /translation="MLIVLEGLDGAGKSTQLKALEAYFLQRTHTVHFMHFPMYDTPVY
                     GTLIARFLRGDSGSIDAVDPYLVSLLFAGNRYEMAGLIRNWLQNGEIVLLDRYVYSNI
                     AFQCAKLESRREREQLRDFILKMEYEHFGIPRPDVNLFLDVPLSFVKEKLQSPRTGED
                     RSYLQGKDDIHEASVEFQQRVRDVYMEQAKNDTSFRIISCSDEQGRMADWKVIFSRIE
                     PYLL"
     gene            8439..9668
                     /locus_tag="PLM68_04165"
     CDS             8439..9668
                     /locus_tag="PLM68_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54593.1"
                     /translation="MRKISRLLLGLLFLFSGFVKAVDPVGSALVFQEYFHAFGVGFLD
                     PLSLVSGIALSVIEFTLGVMVLLDFRKRTAALCMFLFMSFFTLLTLFLAIFDPVQDCG
                     CFGEAVKLTNWQTFFKNLVFWPFALFLFLQRNRTAPVATGATEWPVTVFFALLITSVS
                     VYSYRHLPLIDFRGFRTGNNIAALLEEARTNPEYVFITELIYSRDGEERVFQPDSLPD
                     GTWTFVDSRTIRKATGMRHKVTDFSVRDDAGNQVTDSILSEKSTAMVLLISSEKNLQA
                     GVLGKLRPLVEESRESGRPYYAISALPSVGVHEVLLENGIDMPVYMADLKTVLTVIRS
                     TPGLMVINQGVVLAKYGFRDYPSLQTWKKLVNEDPELVVTRTSINNRLTIQIFVALLL
                     LIIFLLRKGFWFARSRF"
     gene            complement(9669..12605)
                     /gene="infB"
                     /locus_tag="PLM68_04170"
     CDS             complement(9669..12605)
                     /gene="infB"
                     /locus_tag="PLM68_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT54594.1"
                     /translation="MSEKQGIRISKVVKEFNIGIGTLTDFLKKKGIEVDPSPNSKISE
                     EAYALVEKEFDKELQIKEQSRKIILKVKDVAQEEQPVTTTPKVKPEPEHVPAPVPVAK
                     QEPVSTPEPAIKTTPVSKPEPAVKPEPATGAEPAIKHRPAAKHEPEARPETATRTEPE
                     AKPVTDSRIEPDSTAETVITPKTESQPQTPAAAETKHAPGKGSTVVGKIDLTRFDKTK
                     EEENKAVEPSQTPAPKAKTGSKSKEEKKSLHVPTYRTEEPVHIETEVEKLAGPKITGQ
                     IDLSRFDRKKPAEDKDKSKIDKQKVKRERIQKPQRERVDVNKVSARKGENGSRAKGRG
                     KDRFKPIHVEVDEDVIQKQIKETYARMTEGKGKTKGSRHRREKREIVTQRKAEEQERR
                     EMESTLLKVTEFVTVNELATLMDVPVVKVIDACMNLGLMVSINQRLDAEAMVLVAEEF
                     GFRVEFVTAGLQEAVQEEEDDPLDLRERPPIVTVMGHVDHGKTSLLDYIRDANVIAGE
                     AGGITQHIGAYSVLLENGRLITFLDTPGHEAFTAMRARGAKVTDMAIIIVAADDAIMP
                     QTVEAINHATAAGVPMIFAINKIDKQGANPDKIREQLANMNILVEDWGGKHQCQEISA
                     KKGTNIDKLLEKILLEADMLELKASPNKRASGTIIESSLDKGRGYLATILVQNGTLHQ
                     GDVLLSGCCSGKVRAMFNERGQKITEAGPSTPASVLGLNGAPAAGEIFVVMEDEKEAR
                     EVAAKREQLIRIQGLRTQKHITLEEIGRRIAIGNFQELNIIVKADVDGSIEALSDSLI
                     KLTTEEVQVNVIHKAVGGISETDVLLATASNAIIIGFQVRPSVSARKLAEKEEIEIRL
                     YSVIYDAIEDVKKGIEGMLAPVEKEEITATVEVRDIFKISKVGTIAGCMVKEGKITRS
                     AKVRIIRDGIVIYTGMLGSLKRFKDDVKEVQAGYDCGLNIDGYNDIKTGDIIEAYTIV
                     EIKRKL"
     gene            complement(12615..>12823)
                     /locus_tag="PLM68_04175"
     CDS             complement(12615..>12823)
                     /locus_tag="PLM68_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOT54595.1"
                     /translation="EEDVMLDEFNDEIDQWIIDALKQIGCDTAKSVLVIPIPEIVKRA
                     DLEEETVAEVIRILRAEFENDTKE"
BASE COUNT         3135 a         3081 c         3039 g         3568 t
ORIGIN      
        1 ataagaaaac cacggaaaat ttcatcacca tccccattac ccatttttcc ggactggcgg
       61 cttatttgcc cacttctgcc ctgcccagga cgcagactgc tttccgcacc acttcctgga
      121 accggtttct ggagtggttg ccgctttccg attgattttg gaaactccgg gatttttttt
      181 acttttgcgc gccctgagcg gacctttagt aaggtgccat gggatccggg aacaatccct
      241 gattgagtaa cactatcaac taaacaatgt catgaaacaa tttgaaatca acgccaccaa
      301 acgcggtaat tttaccaaaa atgcagtcaa gcaattgcgc agagacggaa tggtaccctg
      361 cgtgctttac ggcaacaaaa cagaaaacat ccacttctcc gtactggaaa aagacctgaa
      421 ggatttgatc tacactcctt catcttacat catctctttg aatattgaag ggaaaaagag
      481 cctgtgcatc ctgcatgaag tgcagtttca tcccgttaca gaaagggtgc ttcatcttga
      541 ttttcttgcc atagataagt ccaggcccat atccataacc ataccggttg tgatatcggg
      601 aaattccgaa ggagtgcgtc agggtgggaa aatgcaggtc ctcaacagaa aacttcttgt
      661 caacgggtta ttgaaagacc tgcctgacca attagatatt gatgttacag acctcacttt
      721 gggcaaaagc atcactgccg gtgacctcaa atttgataac atccggatta cttctccccg
      781 cagcaccatc atctgtatgg tcaaaatgac acgggcctca acttctgctt ctgcagcaac
      841 tgaggaagaa acccaggaaa tacctgccgc agaataggca tttgacagga tcatgtcttt
      901 tctggtaaca ggacttggaa acattggaac tgaatatgcc gggacacgtc ataatatcgg
      961 tttttcggtt ctggatgcct gggctgcatt gcataaaacc agcttttcca cattaaggta
     1021 tggttcactt gcctgtataa agcatgcagg gaagcaggtc tggttgctga agccttccac
     1081 ttatgtgaac ctgagtggta aggctgtccg ttattggatg aaggaagaaa gtgtgcccat
     1141 ggaaaacctg atgatcgtac tggacgatgt ggcattgcct ttcggaaaac tcagaatgag
     1201 ggcaaaggga agtgatgggg gacacaacgg cttgaaaaac atatcattgc ttctggagac
     1261 cgaaggatac gcccgtctca gaatcggggt gggaggtgat ttttccaggg gttaccaggc
     1321 agattatgtc ctgagccatt ggaatgaaca ggaggcaagg gaattgccat tcatatgcaa
     1381 caatgccatt caggcgctgg aactttatat acggcagggg atagaccgcg ccatgaacct
     1441 tgtcaacaca acggatagcg ggaattgagc ttatgcggat agacaaatac ctgtgggcca
     1501 tccgggtttt caagaccagg gccgagtctg tggaggcatg ccggggatca agggttaaga
     1561 ttaatgatca gcctgtgaaa ccatcccggg aagttcgcca gggtgatacc attgttgtca
     1621 gaaagggagc cgtgttttat caatacctgg tactgcagcc tgttgaaagg cgacagcctg
     1681 ccagggatgt gcccctttac gccagggatg tgactccgca ggaagaactt gataaactgc
     1741 agatgcccag ggaaacagtc atcatatacc gtaaaaaagg tacgggaagg cctactaaaa
     1801 aagaacgccg acagctggat gatatcatgg atatcaacgg gtgatctcca ggaacctggc
     1861 tttgccttcc ggaatgatct tccagtaatc gacagatgcg ggaattagca ggcatccatt
     1921 tgctgaacgg taagccatac cttctgtctc aatgcccagc gatccctcca gttccagata
     1981 aactacaaaa ttatctgcag cagatgagtc aatctctgtt tgtttgtcca tctccagcaa
     2041 acgtacggta aaatgaggcg aatcagccag gacacccgaa gtggtcgggt ttggaacgtg
     2101 ctttttgtaa tctatcgcat ccagtgccaa ttccaggtgc attcccctgg cagtttccct
     2161 gttgtgttcg cgcccccagt cgtacacccg gtaagtgatg tccgaaacct gctgcacctc
     2221 ggctatggtc aaaccaccac cgcatgcatg gataagacct gccgggataa agtagcattc
     2281 accctttttt ggaacgatac aattcatcac atccggcaag gtttcatttt cacagcggga
     2341 ataaaaactt tcgggggaca tatcctggtt aaagcccaga tagagccttg ctttgggatc
     2401 agcatccatg atataccaca tttcagtctt tccataggcg tggtgcatcc cggctgccct
     2461 ttcgtcgtcc ggatgcacct gaactgaaag gaagtcattg acggtaagat atttgatcag
     2521 taaagggaac tcatctccgc acatgttata aatcctgtca ccaacaaggt ccgtcaggta
     2581 cacttcaagg gcttcgttga gggtgttccc tttcagaaaa ccattgttga taatggatgt
     2641 atcactatca agtccgctca ggacccaggt ctccgatccc cagacacgtt ctttgagaat
     2701 gggtttaaag ctcaggggat acagtttcgt gttgttcatg gtttataatc aatgatggag
     2761 ccggggcaag gtatttatta aggtatgaca aggatatgac aagtaccacc gcagatgctc
     2821 ccagggttat gaaaaatgga gtgtctccaa ttattgacct gagcgatgca tcagcagcca
     2881 gctggggaaa ccaaatagcc agcaggaacg acaggccgtt gttgatagcg tgcagggcaa
     2941 cacaagtcca ataggaacgt gtcctgtaat aaatccatcc taacagacaa ccaatgagga
     3001 aagccggaat ggcctgccag ggattggcat gtattacacc aaacatcagc gcagaccagg
     3061 ctatggcttt ccagggagcc atatgctgca gcataccctt gagggccaac ccccttagca
     3121 gccactcttc caaaataggt gcagcaatga caacagccac aaaagtagga aggttgctct
     3181 ccgtcattct ttcaaaaatt tccttcatga attcaggcat ttccagcaaa tcggtcaggg
     3241 ggtccaaaag aaccgacaag cttatgataa gcaccgggag taaaagtatg aacaacggca
     3301 gtgggactcg gccaagagcg ggacgaggat ttggataggg cggtctgtta ctaaggactg
     3361 cttcccggaa tttgttacgt gaccttagat acacatataa aaacacaaat gtgaaaggaa
     3421 gcacatatac tatgggagtg ctccagtccg aagcccctgt cagggtaagg gtccccggga
     3481 agatctggtt gataatgaac acgactgaaa cagtaatgat ggtgccggct accaaaaaga
     3541 tggccagcat gatgagccag gattctccca gtccgggccg gtaatgatct agatactttc
     3601 tcatttaaaa tagttgttta cagaaacaat ggatttgggt ataaaccttc cctgtgtaat
     3661 gattgtaacc tggagaccac cccgtcgcgg tcttccctgt acgtgactcc aaaccaactg
     3721 gcatcggtct gaaggacgtg gcaattccct tcccccgatt tgatgatctg gttgaccatg
     3781 gtgggaaggt ggtattccga agccggctcg aggatatgtt gttccaggaa tgctgttacc
     3841 aggcgccttg cttttggata tatatcgggc agaaagcccc agcagttcat ggagcagaga
     3901 tctcccggct gtagttccag cagggttgct tcctgtttgt taccgggtat tgtgcaatat
     3961 atttttccgt tatcgtacct tttgatcttg aaccgttcca ccacatctgt caaaagacct
     4021 tctctgttgc tgtgacaaat cccccttgaa acgggaccag atggagaaag tgtgttttcc
     4081 agccggtaac cgaccatggc acaggaacct ttacccagat ctttccggac caggaaatca
     4141 tgcagcaccc ggaatgcatc gcgcccataa aaatcatctg cattgatgat gccgaaaggt
     4201 tcgttaacgc atcgggaagt tgtcagcaag gcatgtcctg ttccccaagg tttctcccgc
     4261 tctgcaggag ggctgaaggg tgctggaata tccgccagtt cctgtgtgat gaatgcaaaa
     4321 ggaaaactat tgccataacg tgctgcacaa acacggttga actcttccct gaaagattcc
     4381 cgtatgataa agacaattct tccaaatccg gcctgaagtg catcgtatat ggaataatcc
     4441 atgatcgttt ccccgttagg gcccagcgtg tccagttgtt tcaggcctcc gtagcggctg
     4501 cccatgcccg cagccataat aaccagtgta ggtcgcatat tgaaaaacct cttatatttg
     4561 ttctggaaaa ttgattccca tgttaaaacg cttcaaatct gaccggttgt tgaagttcat
     4621 caggaacaga tactttattg tgactatggt attcattgtc tgggtacttt tctttgacaa
     4681 caacaacctt atcagctggg tccgcacact gggacaatac tccgcaaaca agtctcagca
     4741 aagatactac aaagaaaaga tacagcaaac cgaaaacaac ctcaggcaat tacaatccaa
     4801 taaagactct cttgaaaaat tcgccaggga aaactattat tttcaccggc aggacgaaga
     4861 tgtttttatt gtcacgccag gtccatcaga tccctcagat cgtttcccat ataggtagga
     4921 gcaccgtcgc agggcagccc gtccggattg taaaaaaact ggtcaatacc gaaaacctgg
     4981 gcaccttcaa tatctgtcag aaaattatct cccaccatga tcgtctcttt tttcttgcca
     5041 ttgaccgaag tgattgcagc gatgaaaatc tttggattgg gtttgtgata ccctacttct
     5101 tcggaaatga aaatcctgtt ttcaaagaaa ggatcaaggc cgctgtttct gaccttgtgg
     5161 tactgcactt gtttgaaacc gtttgaaaga agagccattt tgcatccttt acgctgaagg
     5221 tattccagca cttcccgggc atacggtacc aggtttgttt tctttggcat caactccagg
     5281 tattgttctc caaagacggg acccagactg ccggcatgag gaacaccggc ctgttccagc
     5341 gttatgacaa accttacatc acgtagcttt tgctgagtta tctctccggc ctcgtacatt
     5401 tcccacaact gcttgttgct ggtacggtac atatcataaa aggccgcagg atccggaatg
     5461 tatgctccca gaccgtgtct ctctatcaat tcggttatgc attccctggc gttcccttca
     5521 aaatcccaca acgtattgtc caggtcaaaa agaaaaaaac ggtacttcat ttagtgaccg
     5581 atataatttt ttggcgtgat ctgcagaagc tcgtccttga cctcctggct tatgtccagg
     5641 ttgcggataa aggcgtgcag gtattcttta tctatgtgca tgttggtgcg ggtgagtacc
     5701 ttgagtgttt cgtaaggctg gggatagttt tcccttctga gaatggtctg gatggcctca
     5761 gaaataatag cccagttctc atcaatgtct ttttcaatgg caggcatatt gagcagcact
     5821 ttttcgaatc ctttttgcaa ggatttgaat gccaacaggg tatgtgccag ggggaccccc
     5881 aggttacgca aaacggtgga gtcagtaagg tcacgctgca accgggaaac aggcaacttg
     5941 gctgccagat ggcaaaagat agcattggca acaccaaagt tgccttctgc attttcaaaa
     6001 tcaatggggt taatcttatg gggcatggca gaagacccta tctcctggtc cttgatgtgt
     6061 tgcttgaaat aattcatgga tacgtaagac cacatatccc tgctcaggtc aatacatatg
     6121 gtgttgatcc tgctcagatt gtgacaaagc gctgctatat agtcgtaatg ctcaatttgt
     6181 gttgtaggaa aagaacgttg tattcccagg gtgctgttca ggaatttatc ggcaaacttg
     6241 tgccagtcaa tctgagggta agcaagattg tgtgcgttga agttaccggt agagcccccg
     6301 aatttgcctg cgaagggaat ctgttcaagg cttttgagct gttcttccag ccggactttg
     6361 aagacctgta tctccttgcc cagcctggtg ggagaagcag gttgcccgtg cgtatgtgcc
     6421 agcataacca caggagccca ctccctggat ctttcttcca gcatatcaat gatctgtcgc
     6481 aacaagggta tgtattcttt ttgtatcccg tgtttcaggc tcatgggaat ggccgtgtta
     6541 ttgatgtcct gtgaagtcag tccaaaatgg acgaattcat agtacttgtc cagacccagg
     6601 gctttcattt tgttcttaag aaagtattca accgatttca catcgtggtt tgttaccttt
     6661 tcctgcgctt tgataacctt ggcgtcggtt atggaaaagt ttttgtacaa attgcgcagt
     6721 atgggaaacc agcgtgaatc cacatctttc aactgcggca aaggcagctt gcatagagct
     6781 atgaaatact ctacttcgac atgaagtctg taacgtatca gtgcaaattc cgaaaaatac
     6841 tgattcaggg gagctgtctg ggcgtagtac ctcccgtcaa tgggagagat ggccagcaac
     6901 ggattgaatt ccataatgaa atgatttatt aatgctcaaa tatactcatt tttctgtata
     6961 tggcaatcat gtcagcggcc tgtgcaacat cgtgaacccg caggatagac acgcctttta
     7021 aaagcaattg cagatgaagg gccgaggtgc tcaccagtgc gttatcaggg gtagtgttca
     7081 aagggatgta tgtcattctt tttcttgaaa tacctgccag caaaggggcg caggggtcac
     7141 ccttctcgtt ttttggaacc agggtatgaa gtctggacaa cacttcatag ttctggtcaa
     7201 catccttgtt gaatccgaat cccggatcca ggatatattc tttgatccca tacctggcag
     7261 cggcatccct gatcctatta aaaaaacggg tgattccctc cggtccgtcg tagcgttcct
     7321 ggtgcatggc aatccacggc aggcccaggg aggctatcaa aggcatcatg gcgttatcat
     7381 aacatcctgc aaaaatatcg ttcacccaga accgtccaag caattcatag gcttttttca
     7441 caattacggg acgaaaagtg tcgatggaaa aaagtgcttc gggaaacttt tcttttgcct
     7501 gtagaagcac aggctctaac ctgttccatt cttccatggg agaaggccat gatgcaccgg
     7561 gcctggtaga acaggcgcct atatccacta tggcagctcc ttctcgcaat atggtttcaa
     7621 tacggttgag agcgcagtcc gtatcctgga cacgggaacc ggcgtagaac gagtcgggtg
     7681 tacagttgac cacccccatt atggcaggaa agcagatgtt cataaaacaa aagtaataaa
     7741 aaagcggaac gattttttat gtagttttgt atcatgttga ttgttcttga aggtctggac
     7801 ggagccggca aatcaaccca gctcaaagca ttggaggcgt atttcttgca acgcacccat
     7861 acagtgcatt ttatgcactt tcccatgtat gacacgcctg tatacggtac tttgatagcc
     7921 cgtttcctca ggggggacag cggttccata gatgcggtgg acccgtacct ggtctcactg
     7981 ctctttgccg gcaacagata tgaaatggcc ggcctgatca gaaactggct gcagaacggc
     8041 gagatagttc tgttggacag gtatgtgtat tcaaacatag cttttcagtg tgccaaactg
     8101 gaaagcaggc gtgaaaggga acaattgcgg gattttatcc tgaagatgga atacgaacat
     8161 tttggaattc ccaggcccga tgtgaacctt tttctggatg tacccctgtc ttttgtaaaa
     8221 gagaaactgc aatctcccag gacaggcgaa gatcgctcct acctgcaggg gaaggatgac
     8281 atacatgagg catctgtgga gttccagcag agggtaaggg atgtttacat ggaacaggca
     8341 aagaacgata catctttcag gatcatttca tgcagtgacg aacagggtag gatggctgac
     8401 tggaaagtta tattttcacg gatagaaccc tacctgctat gagaaagatc tcccggttgc
     8461 tgcttggact cctttttctc ttttcaggtt ttgtaaaagc ggtggatccc gtgggatctg
     8521 ccttggtttt tcaggagtat ttccatgctt ttggagtcgg attcctggac cctttgtccc
     8581 tggtatccgg cattgcgttg tctgtaattg agttcacact gggcgttatg gttctcctgg
     8641 actttcgcaa acgtacagcc gccttgtgca tgtttctgtt catgtctttt tttacgctat
     8701 tgacattgtt cctggcgatt tttgaccctg ttcaggattg cggatgcttt ggcgaggcag
     8761 tgaagctgac caattggcag actttcttta aaaaccttgt tttctggcct tttgccctct
     8821 ttcttttttt acagaggaac agaactgctc ccgtagccac cggggcaact gagtggccgg
     8881 tcactgtttt ttttgcactc ctgattacct ctgtgtcggt atatagttac aggcacctgc
     8941 ccctgattga tttccggggt ttcagaacag ggaacaacat tgcagccctt ctggaagaag
     9001 cccggacaaa tcccgaatat gttttcatca cagaactgat atacagcagg gatggagaag
     9061 agcgtgtatt ccagcctgat tcccttccag acggcacctg gacatttgtt gattcgcgta
     9121 ccataaggaa agctacgggt atgcgccaca aagtcactga tttttccgtg cgggatgatg
     9181 ccggaaatca agtcaccgat tccatactgt cagaaaagtc tactgctatg gtcctgctga
     9241 tctcatcgga gaaaaacctg caggccgggg tgttgggaaa actgaggccg ttggtggaag
     9301 aaagccggga aagtggccgc ccttattatg ccatcagcgc acttccgtca gttggcgttc
     9361 atgaggtgct gttggaaaac ggaatagaca tgcccgtcta catggcagac ctgaagacag
     9421 tcctcactgt aatccgttct acaccgggat tgatggtgat caaccaggga gtggtactgg
     9481 ccaagtatgg tttcagggac tatccttcac ttcaaacgtg gaaaaaactc gtgaatgaag
     9541 atccggaact tgtggttacc cgaaccagca ttaacaacag gctgaccata caaatctttg
     9601 tcgccctgct gttattgata atctttctgc tccggaaagg gttctggttt gcccggtcac
     9661 gcttctgatc agagtttcct cttgatttca acaatagtgt atgcctctat gatgtcacca
     9721 gtcttgatgt cgttgtatcc gtcaatattc agaccgcagt cgtaacccgc ctgtacttcc
     9781 ttgacatcgt ccttgaaacg tttgagcgag cccagcatac cggtgtagat gacaatgcca
     9841 tcacggatta tgcgcacttt ggcactccgt gtgattttgc cttccttgac catacagcct
     9901 gcaatggtac ccaccttgga tattttgaat atatcccgta cctccacggt tgctgtgatt
     9961 tcttcctttt ctacaggggc aagcatgccc tcgatacctt ttttaacgtc ttcaatggca
    10021 tcataaatca ccgagtagag tcgtatttct atctcttcct tttctgccag ttttcttgcc
    10081 gagacagagg gacgcacctg gaaaccaatg attatggcat tggatgccgt ggcaagaagc
    10141 acgtcggttt cagagatacc acctacagcc ttatgaatga cattaacctg gacttcctcg
    10201 gttgtcagct tgatcagtga atctgaaagc gcttcaatgg atccgtccac atctgctttc
    10261 acaatgatat tcaattcctg gaaattccct atggctatgc ggcgtccgat ctcttcaaga
    10321 gtgatgtgtt tttgagttct aagtccctgt atgcgaatta actgctccct ctttgccgca
    10381 acttcccttg cctccttttc atcttccatc acgacaaaga tctctccggc agcaggggcg
    10441 ccgttcaggc cgagaacaga agcgggagta gaaggacctg cctctgtgat cttctggccc
    10501 ctttcattga acatggcacg gacctttccg gagcagcagc cgctcagcag gacatctccc
    10561 tgatgcagcg tcccgttctg gaccagtatc gtcgccaggt aaccccttcc tttatccagg
    10621 gatgattcaa tgatcgttcc cgatgccctc ttattgggac tggcctttaa ttccagcata
    10681 tctgcctcca acagtatttt ttccagcaac ttatctatgt tggttccttt ctttgccgag
    10741 atttcctggc attggtgctt tccgccccaa tcttccacca ggatgttcat attggccagt
    10801 tgctcccgga ttttatccgg attggctcct tgtttgtcga ttttattgat ggcaaagatc
    10861 atgggtaccc cggcagctgt agcatggttg attgcttcca ctgtctgagg cattatggca
    10921 tcgtctgcgg caacgataat gatggccatg tctgtgacct ttgctccgcg tgccctcatg
    10981 gccgtaaagg cctcgtgtcc gggagtatcc agaaatgtta tcaggcggcc gttttccaga
    11041 agcacgctat aagccccgat atgctgcgtt atgcctcctg cttcccccgc tatcacgttg
    11101 gcatcccgaa tgtagtccag cagggatgtc tttccatggt cgacgtgtcc cataacggta
    11161 acgatagggg gcctttcccg caggtcgagt ggatcgtctt cttcttcctg cacggcttcc
    11221 tgcaggccgg ctgtgacaaa ttcaacccgg aagccgaatt cctctgcaac caggaccatg
    11281 gcttctgcat ccagccgctg gttgatggaa accatcagtc ccaggttcat acaggcgtca
    11341 atgaccttga caaccggaac atccatcaga gtagccagtt cgtttacagt aacgaactcg
    11401 gttaccttga gcagtgtgct ttccatttcc cggcgttcct gttcttccgc cttccgctgg
    11461 gttacaatct cacgtttttc acgacggtgc ctggaccctt ttgttttgcc ctttccttcg
    11521 gtcatgcggg cataggtttc ttttatctgt ttctggatga catcctcatc aacttccacg
    11581 tggatgggtt taaacctgtc tttaccgcga ccttttgcgc gggagccgtt ctcaccctta
    11641 cgtgcagata ccttattcac atcaacacgt tccctttgtg gtttctggat cctttcccgc
    11701 ttgactttct gcttgtcaat tttggattta tctttatcct cggcaggttt cttcctgtca
    11761 aaacgtgaaa ggtcaatctg cccggtaatt tttggtccag ccaacttttc aacttcagtt
    11821 tctatgtgaa ccggttcttc cgtcctgtaa gttggaacat gaagggattt cttttcctct
    11881 ttggatttgc tccctgtttt tgctttgggg gccggagttt gggaaggttc cactgccttg
    11941 ttttcctctt ctttggtctt gtcaaaccgg gtcagatcta tctttccaac aacggtggaa
    12001 ccttttccag gggcatgctt tgtttctgca gctgccggtg tttggggctg actttctgtt
    12061 tttggagtaa tgaccgtttc ggctgtagaa tctggttcaa tacgggaatc agttacaggt
    12121 tttgcttcag gttcagtcct ggttgccgtt tccggtcttg cttcaggctc gtgttttgct
    12181 gcaggtctgt gtttaatcgc aggttctgct cctgttgccg gttcaggctt aactgccggc
    12241 tcgggttttg acacaggagt tgtttttatt gccggttccg gtgttgacac aggctcttgt
    12301 ttggcaacag ggacaggtgc cggaacgtgt tcaggctccg gctttacttt cggagtagtt
    12361 gtgacaggtt gttcttcctg tgctacatct tttaccttaa gtatgatctt cctggattgt
    12421 tccttgatct gcaattcctt gtcaaattcc ttttcaacaa gtgcataagc ttcctcggag
    12481 attttactat tgggcgatgg gtccacttca attccctttt tcttcaggaa atcggtgagc
    12541 gtgccaatcc caatgttaaa ctccttgact actttgctta tacgaatacc ttgtttttcc
    12601 gacatataat ctccttattc ttttgtatcg ttttcaaatt ctgccctcag gatgcgtatc
    12661 acttcggcta cagtttcctc ttccagatcg gcacgcttga caatttcagg tatgggtatg
    12721 accagaacgc tttttgctgt atcgcaaccg atttgcttaa gtgcatcgat gatccactga
    12781 tcaatttcat cattgaattc atccagcatt acgtcctctt cat
//
LOCUS       DAOSOF010000049        12756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_4911_length_12756_cov_5.373356, whole genome shotgun sequence.
ACCESSION   DAOSOF010000049 DAOSOF010000000
VERSION     DAOSOF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_12756_cov_5.373356"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLM68_04180"
     CDS             <1..263
                     /locus_tag="PLM68_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54596.1"
                     /translation="QQERTNHAVSLTITRSFAYDVLSAECTGYYNITSKEWFVRPKLS
                     WRISDALMAAVGGNYMRGGEKTLYGYSSAIMNGAFAELKVIF"
     gene            267..2522
                     /locus_tag="PLM68_04185"
     CDS             267..2522
                     /locus_tag="PLM68_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT54597.1"
                     /translation="MGLSNFVIKYRWYIIIVTLVLVLLGIIPLTKIRVNPDLESYLPQ
                     TMPSIQNNKKIGEYFGNDELLLIIFQTEDVLNEKTLNRIRKITDTLSIMPQFGRVYSL
                     FQGKDIRSEEGSMVVDPVVQRIPDNEDDREALRDAIRKNDLVYKVVVSEDFKYALIIL
                     SSDKTVNDDHLMQLIHETLQKYPGDEEIYINGSPFLRDEASKKIGRDIMLLLPIGLLV
                     MFLILYFSFRELRSVLLPFFVVLFSIVISMALIPVFGWEFSLIGVIIPVMMLAIANNY
                     GVYYAARYQDLRASQPDLEASELAKQVSGYLVTPVTLCGLTTIVGILGLVAHLFRPAR
                     QMGIVSAIGISFALLVSLLFIPAVLSLLKKGRIHKDLQDGSKGFIPSLTERAGEGITR
                     HPKRIVGIFILFMAFCSAGLFFFKTAPDSNDVLPQSHSFNKAINIMDNSFGGSKMFNV
                     MFQGDVKDPALLACLDKYEQELKKVPGVGTVTSLSSIIRKISKTLNDSTDAGYDHIPD
                     SREGVAQYLELYSMSGDPQDLEQFVDFDYTSTLLTVQFKAETIGEIRSLLAKIEGMAR
                     EDSLEPVIGGYTLTDVDINESVEKGQYYSLLVAFVAIFILLSLIFKSFQAGLIGSIPL
                     AFAVFCTFGLMGWLGKELDIVTALISSISIGLGVDFTIHVFWRIKWELAHGNNYGRSI
                     KATLKTIGRGIVINASSVMLGFAVLSLSAFPLIRSFALLIILSLFLCLVCALVLIPAI
                     CYWTRPKFLEK"
     gene            2538..3317
                     /locus_tag="PLM68_04190"
     CDS             2538..3317
                     /locus_tag="PLM68_04190"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOT54598.1"
                     /translation="MKLLLKSLFTGLLLLSGTPGLFGQSAAEISSKSREVVDLGTMQM
                     DFTIYIRDSRGSERVRQLTMISRIFNDVNKTLVRFTAPSDVQGTTLLIFDNKDTEDDM
                     WIYMPALKKVRRIISSERGKSFMGTEFTNADMSKPNQADFNYSLLGTADHEGKACWKV
                     QSEGKDQTINKANGFSRTISYIDKANYLCYKVEYYDLSGNLQRILTSHDYKNVTANKY
                     FSYRTVMENVQNKRVSEMVINKIDTGTKFQEDIFSPNALQQ"
     gene            complement(3321..4028)
                     /locus_tag="PLM68_04195"
     CDS             complement(3321..4028)
                     /locus_tag="PLM68_04195"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54599.1"
                     /translation="MDTSQLSSHCIELLRTHNRVSLGSMGSFMAEDTPATLTKNGHIL
                     LPPGRRIFFRESETWNDGLLEEAYGEKEELGALLSDISKKLAREKSVTLHSFGKLRKT
                     REGDVFFVMDKNVSVNPNGFGLVPLSLRPLLVPAKVRCDLPKDTSQAAELQEQETPDQ
                     PVQRTTDGPALPVANKIPQKTTVNKPKKKASTGVLVVLTVILVVILCLILIYIFRDSL
                     KPLLEQILYSAEERQLL"
     gene            4117..4193
                     /locus_tag="PLM68_04200"
     tRNA            4117..4193
                     /locus_tag="PLM68_04200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4151..4153,aa:Asn,seq:gtt)
     gene            4318..4506
                     /locus_tag="PLM68_04205"
                     /pseudo
     CDS             4318..4506
                     /locus_tag="PLM68_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            4715..6142
                     /locus_tag="PLM68_04210"
     CDS             4715..6142
                     /locus_tag="PLM68_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT54600.1"
                     /translation="MTVQLIITLCVLLLFAYIFDISAARTKIPSVVLLLLLGWGVRQG
                     TILLDLQIPNLMPLLPFLGTMGLILIVLDGAMELEVDRSKLPLVGKSFFMALLSMVII
                     SGLLGWIIYRIDGIVSFKDALANAVPFAVISSAIAIPSSRNLGAANREFVTYESSFSD
                     ILGVVLFNFFVFNKVIDAGSVIHSLTGFLIILLVTVVATAGLIFLLSKIKHHVKFVPI
                     ILMIILIYAVTEIWHLPALLFIILFGLIIGNINVLRRHRLIRRLQPDALCAEVKRFHE
                     LITEFTFLVRASFFLLFGFLLETSELLNSHTIVWALLIAAGIFLFRYLLLKIFRLPAQ
                     ELWFIAPRGLITILLFLSIPMEQSSALVNKSLMVQVVIISALVMMGGSLFPKGKTPGS
                     PQAEAEPDAPDSDEPEQAEPDAPDSDEPEQAESDAPDSDEPEQAEPSEPVVSGSAEFG
                     KAAPGPTEPSSSGQANPECRARPNL"
     gene            complement(6132..6668)
                     /locus_tag="PLM68_04215"
     CDS             complement(6132..6668)
                     /locus_tag="PLM68_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54601.1"
                     /translation="MKNVLCVKNLVKFQLGILVILLISSCNKPLTRDVAKDLIIQKYE
                     FPQADIRNYKLSTTKEYDLISTKEKIRTSDYTPAKLKFLSELENAGFITYSFDLVSAN
                     VYGSRWGDVGDEAGRGFTQEVKNFTGEKTGRRHTCLIKAIYKHSGILTRVGKPGVPRK
                     RNFEIFKGLIFWCLIFTN"
     gene            6703..7308
                     /locus_tag="PLM68_04220"
     CDS             6703..7308
                     /locus_tag="PLM68_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT54602.1"
                     /translation="MEVFQPVNIVPGLTVFGLTFITMTLGMLIAKNKSSSFLTRLFIS
                     PLKPRDFILRYFIPVMPMTAIITVSCLVTGLLVGVPFSVKLLYTFLSFIPFVLFSVFF
                     GIFLGITCRETQVLALGNIYIISSTLLGGAWMDLRILGETIEKITDLLPFSHAIEASR
                     IVLSGRQDDTWVHLGVACLYALVFFMLAVVFFRRKMRSDNK"
     gene            complement(7323..7880)
                     /locus_tag="PLM68_04225"
     CDS             complement(7323..7880)
                     /locus_tag="PLM68_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT54603.1"
                     /translation="METLNLIFMAIGLAMDTFAVSICKGMGHSRMNWLNALKAGLIFG
                     GFQALMPLLGYRAGSRFQNQIEAADHWIAFGLLVFIGGKMIVESFRHDKPVDCSFGIR
                     SMLPLAIATSIDALAVGISLGFLQTDIVKAVILIGSVTFLFSFAGVKLGHVFGSRFSS
                     KAEVIGGIILVLIGVKIVLEHTGIL"
     gene            7968..9077
                     /locus_tag="PLM68_04230"
     CDS             7968..9077
                     /locus_tag="PLM68_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT54604.1"
                     /translation="MSLNTCSLKKCILFAILSLWFVQAKGSGHLPVSVPKPRITIIAT
                     GGTIAGMASSATGTSYDPAQLTVDALMAQAPGISETATITGMQLCNIASQHMTTEIWI
                     RLASMIDSLFKNSICDGVVVTHGTDTMEETAFFLDLVLGYDNPVVLTGAMRPANALGA
                     DGPANLFNAVCLAAHPAARNRGVMAVMNDNIYSAAEFTKVHTVNPDAFKSPDHGPLGL
                     IRGGNPLFFREPAGRLWTQLTCNTEELQELAEKQSKKFFPRVEIILSYAGATAGLIDA
                     LLNSGVDGIVVAGVGHGNYSQEIEAGIQHCLEQGVFVVRATRVLAGGVTFDVENPFDG
                     QIVSGMLSPQKARILLMLALTRFNTTEQLQKIFSR"
     gene            complement(9109..11142)
                     /gene="metG"
                     /locus_tag="PLM68_04235"
     CDS             complement(9109..11142)
                     /gene="metG"
                     /locus_tag="PLM68_04235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT54605.1"
                     /translation="MKQQEFKRYLVTSALPYANGPIHIGHLAGVYIPADIYVRYLRLC
                     GREVCFVGGSDEHGVPITIRARQQGVTPQDIVDKYHFQIRESFSRLGISFDVYSRTSS
                     KIHYKTASDFFRHLYDNEKLLEKETEQFYDPETQTFLADRYIVGTCPHCSYSDAYGDQ
                     CEKCGSTLSPDELINPRSSISGSVPEKRMTKHWYLPLDAYEPWLRKWILEGHKEWKSN
                     VYGQCKSWLDNGLQPRAVSRDLDWGVPVPVEGAQGKVLYVWFDAPIGYISNTIELFPD
                     SWEKWWKDKNTKMVHFIGKDNIVFHCIVFPAMLKAHGGYILPDNVPANEFLNLEGDKI
                     STSRNWAVWLHEYLNELPGKEDVLRYTLCSTMPETKDNDFTWKDFQARNNNELVAILG
                     NFVNRAVVLTHKYFNGLVPAAGIPTDYDQEVMRSVPGIASALEDNLEHYRFREALKEA
                     MNLARLGNKYLADTEPWKVAKTDMERVATILNFSLQLCARLTVALDPFLPFTTIKLRN
                     ILNMPQALSWQDLARETLLPGGHRLNEPVLLFDKIDDATIETQMAKLKKTEPVKKDVA
                     PALPECSFADFEKMDIRVATVLQSERVPKTDKLLKLTIDTGLDQRVIVSGIAQYYTPE
                     DMVGKQICILANLAPRTIRGIVSQGMILMAKEPGGNMRLISPLEGISDGAVVG"
     gene            complement(11157..>12756)
                     /locus_tag="PLM68_04240"
     CDS             complement(11157..>12756)
                     /locus_tag="PLM68_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOT54606.1"
                     /translation="RDSLLLVNGYARDFYSNYLWEEKAGQDAGLSYFRERGFTEHVIR
                     HFQLGYAPAGKDTFTRAALAAGYKPELLIATGLTIHFPERDNKPEAFMDRYQDRVIFP
                     IHSLSGRVIGFGGRTLRSDKQVSKYINSPDSEVYNKSRSLYGIYYAKQDIVKGGKCYL
                     VEGYTDVISLFQAGITNVVASSGTSLTVEQIRLIKRFTQKVTVLYDGDDAGIRASIRG
                     IDMLLEEGLQVKVVRFPQGDDPDSFARSHNARETLDFLEKNETDFIHFKIALMADEMK
                     DSLKKSALIREVVESIAIIPDAITRTVYIEECSRHLDIEEMILTREVAKIRKKRTEWV
                     NRPSSYPPPPPLPPGEENIQEAELKPGSGELSRCEQDILYYLIKFGQTMIEDMTVSEF
                     IQRALAEDELEFQDPFFAGIFREYATLTGDDEARKKFFIHHESPLISQHVLDLISNPH
                     PINVKVLRESLPLEEPMLKENVTKALLVYKLKIVSLSCADLTRDLRQAQQLGEEEKIQ
                     TILKELAVMMDVRNSFARELNRLK"
BASE COUNT         3187 a         3041 c         3038 g         3490 t
ORIGIN      
        1 atcagcagga aaggaccaac catgcagttt ccctgaccat aaccaggtct tttgcttacg
       61 atgtcctgtc agctgaatgt acaggctatt acaatatcac atcaaaagaa tggtttgtaa
      121 ggcccaagtt gagctggagg atatcggatg ccctgatggc cgctgtaggt ggcaattata
      181 tgaggggggg agaaaaaaca ttgtacggct attcttcagc cataatgaac ggggcttttg
      241 cagaacttaa agtgattttt taaaacatgg gactatctaa tttcgtcatt aaatacaggt
      301 ggtatatcat aattgtcacc ctggtactgg tactgctggg gattattccc ctgacaaaga
      361 tccgtgtcaa tccggatttg gaatcttacc tgccccagac catgccgtcc atacaaaaca
      421 ataagaaaat cggcgagtat ttcgggaatg atgagttgtt gctgattatt ttccagacag
      481 aagacgttct taatgaaaaa acgctgaaca ggatcaggaa gataacggat accctgtcca
      541 taatgccaca attcggtcgc gtctactcgt tgttccaggg aaaggatatc cgttcggaag
      601 agggaagtat ggtggtggac cctgttgtgc aaagaattcc cgataatgag gacgaccgtg
      661 aggcactcag ggatgcgatc cgcaagaatg atctggtata taaagtagtg gtttctgagg
      721 atttcaaata cgcactgata atcctgagct cagacaaaac ggtcaatgac gaccacctca
      781 tgcagctgat ccatgaaaca ctgcaaaaat accccggaga cgaagaaatc tatatcaacg
      841 ggagtccctt tttacgggat gaggcgagca agaaaatcgg ccgtgatatc atgctgcttt
      901 tgccaatagg cctgctggtg atgtttttga tcctgtattt ttcttttcgg gaactcagaa
      961 gtgtgctgct gccatttttc gtggtgttgt tttccattgt gatttccatg gccctgattc
     1021 cggtttttgg atgggaattc agcctgatag gtgtgatcat tcctgtcatg atgcttgcca
     1081 ttgccaataa ttacggggta tattatgctg cccgctacca ggacctgcgg gcatcgcaac
     1141 ccgatcttga agccagcgaa ctggccaaac aagtatccgg atacctggtt actcctgtca
     1201 ccttatgcgg actgaccaca atagtgggaa tcctgggcct ggtggcacat ttgttcaggc
     1261 ccgccaggca gatgggtatc gtctcggcca taggcatttc ctttgcgctg ctggtgagct
     1321 tgctgttcat cccggcagtt ttgtccctgc tgaagaaagg cagaattcac aaagacctgc
     1381 aagacggttc gaaaggtttc attccttctt taaccgaacg tgccggggaa ggcattacac
     1441 gtcatcccaa aagaatagtt ggcattttca tactctttat ggcattctgc tctgcaggat
     1501 tgttcttttt caagacagcc cctgattcca atgatgtttt gcctcaaagc cattcattca
     1561 acaaggccat aaacatcatg gacaacagct ttggagggag caaaatgttc aacgtgatgt
     1621 tccagggtga tgtgaaagat cccgcgttgc tggcatgcct ggataaatat gagcaggaac
     1681 ttaaaaaagt gccgggtgtg ggaacagtca cctctctttc ctccatcatc aggaagataa
     1741 gcaaaaccct gaacgattcc accgatgccg gttatgacca catacccgat tccagggagg
     1801 gtgtggccca atacctggaa ctctattcca tgagcggaga tcctcaagat ctggagcaat
     1861 ttgtggattt tgattatacg agtacactgc tcactgtgca gttcaaagcc gagaccatcg
     1921 gggagatcag gagcctgctt gcgaaaatcg aggggatggc ccgggaagat tcactggaac
     1981 cggtcatagg gggctacaca ctgaccgatg tcgacataaa cgagtctgta gagaaaggcc
     2041 aatactattc cctgctggtg gcctttgtgg cgatatttat tttactgagc cttattttca
     2101 aaagctttca ggcaggtctg ataggaagca ttccactggc ctttgccgta ttttgcactt
     2161 ttggtttgat gggctggctt ggtaaggaac tggatattgt taccgccttg atttcatcaa
     2221 tatcaatcgg tcttggtgtg gatttcacca tccatgtatt ctggaggata aaatgggaac
     2281 tggcacacgg caacaattat ggaaggagta tcaaggcaac gctcaaaacc atcggaaggg
     2341 gtatagtcat taacgcaagc tcggtaatgc tgggttttgc tgttttgtct ttgtctgcat
     2401 ttccacttat ccgctctttt gcccttctga taatcctgtc actcttcctg tgccttgtct
     2461 gcgcgctggt cctgatccct gccatatgtt actggacaag gcccaaattc ttagaaaaat
     2521 aaaggaaact caatactatg aaactactgt taaaatcgct tttcaccgga ctgcttctct
     2581 tatcgggcac ccccgggtta tttggacaat ctgcagctga aatcagcagt aaatcgagag
     2641 aggtggttga cctcggcacg atgcaaatgg atttcaccat ttatatcagg gacagcaggg
     2701 gaagcgagcg ggtaaggcaa ctgacaatga tatcgcgcat tttcaatgat gtgaacaaga
     2761 cactggtacg ctttaccgcg ccgtctgatg tacagggtac gacattgctt atatttgaca
     2821 acaaggacac agaagatgac atgtggatct atatgccggc cttgaagaaa gtacggagga
     2881 tcatcagttc cgaacggggc aaaagcttta tggggactga gtttactaac gccgatatga
     2941 gcaagcccaa ccaggcggat ttcaattact ccctgctggg cactgcagat catgagggca
     3001 aagcatgctg gaaagtccaa tcggaaggaa aagatcagac aattaataaa gctaacggct
     3061 tcagcaggac aatcagctat atagataaag ccaactactt gtgctacaaa gtggaatact
     3121 atgacctgtc tgggaatttg cagaggatcc tgacttctca cgattataaa aacgtgacgg
     3181 ccaacaaata tttttcctat aggaccgtca tggaaaatgt tcagaacaag cgtgtgtccg
     3241 aaatggtcat caacaaaata gatacaggaa cgaaattcca ggaggatatt ttctctccca
     3301 atgcactgca gcaataatag ctaaagaagc tgccgctcct ctgcagaata aaggatctgc
     3361 tccagcaagg gttttaggga atccctgaaa atgtagatta ggataaggca caatatgacc
     3421 accaggatca ctgtaagaac taccaaaaca ccggtggatg cttttttctt tggtttattt
     3481 acggttgttt tttgtggaat cttatttgcc acgggtaagg ccggtccatc cgttgttctt
     3541 tgaaccggtt ggtccggtgt ttcctgctcc tgcagctctg cggcctgaga cgtgtctttt
     3601 ggcagatcgc acctgacctt ggcagggacg agcagcggac gcagggacaa gggaaccagc
     3661 ccgaaaccat ttggattgac agagacgttc ttatccatga caaaaaaaac atctccctcg
     3721 cgcgttttcc taagcttccc gaaactgtgc aatgtaaccg atttctccct ggccagcttt
     3781 ttggaaatat cagaaagcaa agccccgagt tcttctttct ctccatatgc ctcttccagc
     3841 aggccgtcat tccatgtttc gctttcgcga aaaaaaatcc gtctgcctgg aggaaggagt
     3901 atgtggccgt tcttggtaag tgtggcgggg gtatcttctg ccatgaaact acccatgctt
     3961 cccaatgaaa cccggttgtg tgtacgaagc aattctatgc aatgggatga aagctgggat
     4021 gtgtccataa gttggacaaa tttacacttt ttttactaag attttgctct ttcaggaaaa
     4081 accgtttatc tttgcactcc ctaaaaggaa aaaaattcct cattagctca gttggttaga
     4141 gcacctgact gttaatcagg gggtccaagg ttcaagtcct tgatgaggag ccacagttta
     4201 aagcacttac gaaaaacgta ggtgcttttt ttattgaaaa aatcatatgt ttttggggcc
     4261 aaaacatggt gttatgtaaa ccaatatgta aaccggaaca caacacttaa ctagctgatg
     4321 aacacgatta tagatgttgt atgctatcgc tcaaaaaccc tgtcaagtgg agaatcgcca
     4381 ctaatgctga gattaaccaa ggaagggaaa agaaaatatg taagccttca aatctcctta
     4441 aagcctgatt attgggattt taacaaaaac aagccaagga gaaactgccc tgagaaagac
     4501 tcaataaact tcagctacct gtattgtggc atgaacgcaa aatgtgccta cgaaataact
     4561 ctttgtaaac aatttaaaat gcaattacac gtgggagtac agaacgcgac tcgggctata
     4621 tttacggtcc ctcgctgccc gggagcgtaa ttatgagtat ccgttttgat atataattat
     4681 ttatatttgt tatggcagat aagaatgtta tgctatgacc gttcagctta ttatcaccct
     4741 ttgtgtacta ttgcttttcg catatatttt tgatataagc gctgcaagaa caaaaattcc
     4801 ctcggttgtt cttttgctct tattgggttg gggagtcaga cagggtacga ttttgcttga
     4861 cctgcagatc cccaatctca tgcccctgtt gcctttcctt ggcaccatgg ggcttatact
     4921 cattgtcctg gatggagcca tggaactgga ggtggacagg tcaaaacttc cactggtggg
     4981 taaatccttt tttatggccc tgctgtccat ggttattata agcgggctcc tgggctggat
     5041 aatataccgg atagacggga tcgtttcatt taaggatgct ttggccaatg cagttccttt
     5101 tgccgttatc agcagtgcca ttgctattcc gagttccagg aatctgggtg ctgccaatcg
     5161 tgaatttgta acctacgaaa gcagcttctc cgacatcctg ggtgttgtcc tgttcaactt
     5221 ctttgttttc aataaggtaa ttgatgccgg atctgtgatc cattccctca cgggattcct
     5281 gataattttg ctggttaccg tggtggctac tgccgggctg atcttccttc tgagcaaaat
     5341 aaagcaccat gtgaagtttg ttcccattat tctaatgatc attctcatct atgccgtgac
     5401 cgagatctgg catttgcccg ctttgctgtt cattatcctt tttggactta ttattggtaa
     5461 cattaatgta ctcagacgcc accggttaat caggcgctta caacccgatg cgctgtgtgc
     5521 cgaagtaaag cgcttccatg aactgatcac tgaattcact ttcctggtac gcgcatcgtt
     5581 cttccttctc tttggctttc ttctggaaac ctccgagttg ctcaattcac acaccattgt
     5641 gtgggccctt ctgattgccg caggcatctt tttgttccgc tacctgctcc tcaaaatctt
     5701 cagacttcca gcccaggagt tatggtttat tgctcccagg gggcttataa ccatactgct
     5761 tttcctttcc atccctatgg agcaatcctc ggcactggta aacaaatcgc ttatggttca
     5821 ggtcgtgatt atctcggccc ttgtcatgat gggtgggtct ttgttcccca aaggcaaaac
     5881 acccggaagt ccgcaggccg aggccgagcc cgatgctcct gattctgatg aacctgaaca
     5941 agccgagccc gacgctcctg attctgatga acctgaacaa gccgagtccg acgctcctga
     6001 ttctgatgaa cctgaacaag ccgagccctc tgaacccgtt gtgtccggat ccgccgaatt
     6061 cgggaaagcc gctcccgggc ctactgaacc ttcctcatcc ggtcaggcaa acccggagtg
     6121 ccgcgcaagg cctaatttgt gaaaattaaa caccagaaaa tcaggccttt gaaaatctca
     6181 aaatttctct tgcgcggcac tccgggtttg cctacgcgcg ttaatatacc actatgtttg
     6241 tatattgctt taataagaca tgtatgcctt ctacctgttt tttccccagt aaaattttta
     6301 acttcttggg taaaaccccg gcctgcttca tcaccaacat caccccatct gcttccataa
     6361 acgttagcag atacaaggtc aaaagaataa gtaataaatc cggcattttc taattcactt
     6421 aaaaatttaa gtttagctgg cgtataatct gaagttctaa ttttttcttt tgtcgaaata
     6481 aggtcatatt cctttgtcgt agaaagttta taattacgga tatccgcttg tgggaattcg
     6541 tatttttgaa taattaaatc ctttgctaca tcacgagtta gtggcttatt gcaacttgaa
     6601 attaatagga ttacgagaat acctaattga aatttaacaa gatttttgac gcaaagcaca
     6661 tttttcataa tcctgtttta aatcatatcc cggaatgttc ccatggaggt gtttcaaccg
     6721 gttaatattg ttccggggct gacagtgttc gggcttacct ttattaccat gactctcggg
     6781 atgcttatag ccaaaaataa aagttcatct ttcctgacac gtttgttcat atcacccttg
     6841 aaaccacggg atttcattct gaggtatttt atccctgtaa tgccaatgac tgctatcata
     6901 acagtgagct gccttgttac cggactttta gtaggagtgc ccttttcggt caaactcctt
     6961 tacacatttc tttcctttat tccctttgtg cttttctcgg ttttttttgg gatatttctg
     7021 ggaataacat gccgggaaac gcaggtactg gctttaggta atatttacat catttcaagt
     7081 acattattgg gcggtgcatg gatggacctg agaatcctgg gagaaacgat agaaaaaata
     7141 acggatcttt taccgttttc tcatgccata gaagcttccc gtattgtatt gtccggccgg
     7201 caggatgata cctgggttca tctgggagtc gcctgccttt atgctcttgt ttttttcatg
     7261 ctggccgtgg ttttttttcg cagaaaaatg agatcagata acaaatagcc acctgccttt
     7321 atctaaagga tcccggtgtg ttccagcacg atcttcacac ctatcaggac caggatgatt
     7381 cctccgataa cctcggcttt tgaactgaag cgggagccaa acacatgtcc cagtttcacc
     7441 ccggcaaaag aaaaaagaaa agtgaccgag ccaatcagta taacggcttt gactatatct
     7501 gtttgaagaa aacccaggga aatgcctact gccagggcat ctatgcttgt ggcaatggcc
     7561 agtggcaaca tggaacgaat cccgaacgaa cagtcaacgg gcttgtcatg cctgaaagat
     7621 tccacaatca ttttacctcc gatgaacacc agcaatccaa aagcaatcca gtgatcagcc
     7681 gcttctatct gattttgaaa cctggatcct gcccggtaac ccaacaacgg cataagtgcc
     7741 tgaaagcctc cgaagataag gccggctttc aaagcgttca accagttcat ccgggaatgt
     7801 cccataccct tgcatatgga tacggcaaaa gtgtccatgg ccaggcctat ggccataaag
     7861 atcaggttga gtgtctccat ttggaaaaat taccatatga ggagcggtca aacatttaga
     7921 aattccttat ttttacatta aagaaaacaa aggtaagaaa actgatcatg tctctgaata
     7981 catgtagcct gaagaaatgt atattatttg caatcctctc cctatggttt gtccaggcaa
     8041 agggcagcgg ccacctgccg gtatctgttc ccaagccgag aataaccata atagcaactg
     8101 gcgggaccat agccggaatg gcttcttctg caacgggtac atcatacgat ccggcccagc
     8161 tcaccgtgga tgccttgatg gctcaggctc cagggatatc agaaaccgcc accataaccg
     8221 gaatgcaact gtgcaacatt gccagccagc atatgacaac ggaaatatgg atccgcctgg
     8281 cctctatgat tgattccctg ttcaaaaaca gcatatgcga cggcgtggtg gttacacatg
     8341 gcaccgacac catggaagaa acggctttct tcctggacct ggtactaggc tacgacaatc
     8401 cggtggttct aacaggagcc atgaggcctg caaacgcact gggcgctgac ggccccgcca
     8461 acctgttcaa tgccgtttgc ctggcagccc atcctgctgc ccggaacagg ggggtgatgg
     8521 ctgtgatgaa cgataatatt tattccgcgg ctgaattcac aaaagtgcat acggtcaatc
     8581 ccgatgcctt taaaagcccc gatcacggcc ctcttggttt aatacgtgga gggaatcccc
     8641 tctttttcag ggaaccggca ggccgcctct ggacacaatt gacatgcaat acagaggaac
     8701 tgcaggaact ggcagaaaaa cagagtaaaa agttttttcc ccgggtagaa atcatcctct
     8761 cctatgccgg agccacagca ggtcttatag atgcgttgct gaatagcggc gtggacggga
     8821 ttgtcgttgc tggcgtcggg catgggaatt attcacagga aattgaagcg ggcattcaac
     8881 actgcctgga acagggcgtc tttgttgtcc gcgccacgcg agtattggcc ggcggagtga
     8941 cgtttgatgt agaaaatcct tttgacgggc aaatcgtaag cggcatgctc agtccgcaaa
     9001 aagcaaggat cctgctgatg cttgccctga cgcgtttcaa caccacagaa caactacaaa
     9061 agattttcag caggtaacag gtatagtcgc gtatgaaatg ttctgttact aacctactac
     9121 ggcgccgtcg gatatgcctt ccaggggaga tatgagccgc atattgcctc ccggttcttt
     9181 ggccatcagg atcattccct gcgatacaat accccggatt gtcctggggg ccaggttggc
     9241 caggatgcag atctgtttcc cgaccatgtc ttccggcgtg taatactggg caatgcccga
     9301 aacgatgacc ctttggtcca gtcccgtatc tattgtcagc ttaagcaatt tgtccgtttt
     9361 gggaaccctt tctgactgca acacggtagc tacccggatg tccattttct caaaatcggc
     9421 aaaactgcat tcaggaaggg ccggggccac atcctttttt accggttcgg tctttttcag
     9481 ttttgccatc tgggtttcaa tggtggcatc gtcaatcttg tcgaatagca acacgggctc
     9541 gttcagccgg tgcccgccgg gcaacagagt ttcccgggca aggtcctgcc atgacagggc
     9601 ctggggcata ttcagaatgt ttctgagttt gatcgtggta aatggcagga agggatccag
     9661 tgccactgtc agccgtgcac agagctgcag tgaaaagttc aggatggttg ccacgcgctc
     9721 catatcggtt ttggccacct tccagggctc ggtatcggcc aggtatttgt ttcccagccg
     9781 cgccaggttc atagcctcct tcagggcttc cctgaaacgg taatgttcca ggttgtcttc
     9841 cagggccgag gctatccccg ggacagaccg catcacttcc tggtcgtaat ctgtaggaat
     9901 acctgcagcg ggaacgagtc cgttgaaata cttgtgggta agaacaacgg cccggttcac
     9961 gaaattaccc aggatggcca ccagttcatt gttgttgcga gcctggaaat ccttccaggt
    10021 gaaatcattg tcctttgtct cgggcatggt ggaacagagg gtgtaacgca aaacgtcctc
    10081 tttgccgggg agctcgttca ggtattcgtg aagccatacg gcccagttgc gcgaggttga
    10141 gatcttatcg ccttccaggt tcaggaattc gttggccggt acattgtccg gcaggatgta
    10201 tcctccgtgc gccttgagca tggcagggaa tacgatgcag tgaaatacaa tgttgtcttt
    10261 gcctatgaaa tggaccattt tggtgttctt gtccttccac cacttttccc agctatcggg
    10321 aaacagttct atggtatttg atatgtatcc tataggcgca tcgaaccaga catagaggac
    10381 ttttccctgg gcgccttcca ccgggacagg aacgccccag tccaggtcgc ggctcaccgc
    10441 cctgggctgc aatccgttgt ccagccatga tttgcattgc ccgtaaacat tggatttcca
    10501 ttctttatgg ccctccagga tccatttcct gagccagggc tcgtatgcat ccaggggaag
    10561 gtaccagtgt ttggtcatcc ttttctcggg aacgcttccg ctgatggaag aacgggggtt
    10621 gatcagctcg tcgggggata gggtggaacc gcatttttca cactggtccc cgtacgcatc
    10681 actgtaggaa cagtgggggc atgtccccac gatataccgg tcggccagga atgtctgggt
    10741 ttcggggtca tagaattgtt cggtctcttt ttccagcagt ttctcgttgt cataaagatg
    10801 tctgaaaaaa tccgaggccg tcttgtaatg gatctttgag cttgttcttg aatacacatc
    10861 aaaggatatg cccagccggc tgaacgattc cctgatctgg aaatggtatt tgtccacaat
    10921 atcctgaggc gttactccct gttgcctggc acggattgtg ataggaacgc catgctcatc
    10981 ggatccgcct acaaaacaga cctccctgcc gcaaagcctt aggtaacgca cataaatgtc
    11041 ggccgggatg tagacaccgg ccaggtgtcc gatatggatg ggcccgttgg cataaggtaa
    11101 ggccgatgtg accaggtaac gtttgaattc ttgctgcttc atcgaaaaaa gtttatttat
    11161 ttcaatcggt tcaactcccg tgcaaatgaa tttctgacat ccatcatgac agccagttcc
    11221 ttcagtatgg tttggatttt ttcttcttca cccagctgtt gcgcctgtct caggtccctt
    11281 gtcaggtccg cacaggaaag cgacacgatt ttcagtttat acaccagcag ggcctttgtg
    11341 acattttcct tcaacatggg ctcttccagg ggcaggctct ccctcaatac cttcacattg
    11401 atgggatgcg gattgcttat cagatccagt acgtgttggg atatcaaagg actttcgtga
    11461 tggatgaaaa atttctttct tgcttcgtca tctcccgtaa gagttgcgta ttcacggaat
    11521 atgcctgcaa aaaacgggtc ctggaattcc agctcgtctt ctgcaagggc cctttgtatg
    11581 aactcagaca ctgtcatgtc ttcaatcatg gtttgcccga atttgatcag gtaatagaga
    11641 atgtcctgct cgcaccggct caactcaccg ctccccggct ttagttccgc ttcctgtatg
    11701 ttttcttcac cggggggcag gggtggcggt ggcgggtagg aagaaggacg gtttacccat
    11761 tcggttcttt ttttccggat tttggccact tcccgcgtaa ggatcatttc ttcgatatca
    11821 agatgacggc tgcattcttc tatgtaaacg gtcctggtga tggcatcggg gatgatggca
    11881 atgctttcca ccacttcccg tatcagggcc gattttttca gggaatcctt catttcatcg
    11941 gccatcagtg cgatcttgaa atgaatgaaa tcggtctcgt ttttttccag gaaatcaagt
    12001 gtctcgcggg cgttgtgaga gcgggcgaaa gaatctggat cgtccccctg cgggaacctg
    12061 accactttca cctggagccc ttcttccagc aacatgtcta taccccggat ggaagccctg
    12121 atcccggcat cgtccccgtc ataaagcacg gtgaccttct gtgtgaaacg tttgatcagc
    12181 ctgatttgtt caacggtcag cgatgtcccc gaagaggcga caacgttcgt gattccggcc
    12241 tggaacagag agatgacatc cgtatagccc tcaacaagat agcatttccc gcccttcacg
    12301 atatcctgtt ttgcataata gatcccgtac agggagcggc ttttgttata gacctcgcta
    12361 tcgggggagt tgatgtattt ggacacctgc ttgtcgctcc tgagggtcct tcccccaaag
    12421 cctatgaccc ttccgctcag cgaatggata gggaatatga cacggtcctg gtagcggtcc
    12481 atgaaggctt caggtttgtt atcgcgttca ggaaagtgaa tggtcagacc tgtggcaatg
    12541 agcaattccg gtttgtatcc ggctgcaagg gctgcccggg tgaaggtgtc tttgccggca
    12601 ggggcataac ccagctgaaa atgcctgatc acgtgctcgg taaacccgcg ttccctgaag
    12661 tagctcagcc cggcgtcctg cccggctttt tcttcccaca agtaattgga atagaaatca
    12721 cgggcatagc cgttgaccag caaaaggctg tccctt
//
LOCUS       DAOSOF010000050        12573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5003_length_12573_cov_4.358763, whole genome shotgun sequence.
ACCESSION   DAOSOF010000050 DAOSOF010000000
VERSION     DAOSOF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5003_length_12573_cov_4.358763"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PLM68_04245"
     CDS             <1..1058
                     /locus_tag="PLM68_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT54607.1"
                     /translation="WRASTHWIGGMGVVLFMLLILPEVSSIRLKLSRIEISSLSQESY
                     HFRAKESVRVIAYVYFGLTILETIALWISGMNLFDAVCHSFATIATGGFSTRNNSIAS
                     YDSVLIETIIIIFMTLSALNFGMIFLLVAKGSLMLFKSPVTRYFLSVLLLGSILVSLN
                     LGLTKTYDSLGESFRFGFFQTISLATSTGFASADSSMWPNFAILLLIFFTLQCACSGS
                     TSGGIKADRILIAFYSIKAHITKRLHPRSVVPTRVGGLAIDPDTVSGVNLFIVFYLFC
                     VLIASLLLTLTGVDIMDAVSATAAHIGNVGPGFGSVGSMSNYSSFNSFAKFVLSVIMI
                     MGRIELFGFLILFSSRR"
     gene            1069..2466
                     /locus_tag="PLM68_04250"
     CDS             1069..2466
                     /locus_tag="PLM68_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT54608.1"
                     /translation="MAIDQFIFEKLRENLPFAPSSDQDVFFRAIASFLSGPNPVFILN
                     GYAGTGKTTAMAALVGALSELNRQVVLLAPTGRSAKVLMGYAGAPAHTIHKAIYRQKP
                     PASSDEGGFGVFELHPNRDKNTLFIVDEASLLSNTPGGMLFGSGNLLEDLVAYVFNGK
                     GNQLLILGDSAQLPPIGLELSPALDPGYMEQYGSVVYMEMKEVLRQSFDSGILYNATL
                     LRDNITAGDYCLPEWKTAGFNDVRRIESSELMECLNDAISRYGLEEVVVLCRSNKRAN
                     RYNKGIRGSILYREEGITKGDRVMVVKNCYQFLEDVPEMDFIANGDIAEILRIYKFHE
                     RYGFRFAEAVLRFPDYKETEISARLLLDTLESESPALSREQQEQLYQGVYSDYEHIKG
                     KRKRYNAVREDLYLNALQVKYANAVTCHKAQGGQWKAVFVDKAFFGEICDKDMLRWYY
                     TAFTRAREQLYLINL"
     gene            2477..4549
                     /locus_tag="PLM68_04255"
     CDS             2477..4549
                     /locus_tag="PLM68_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT54609.1"
                     /translation="MRRYAISLILLFLLPLAIRSQERYLLETPPQGLTKPIDTHIFWK
                     DNRTLLLRSGSQQEPTYYLEDIVSGKRVRTDDPADVSTAGIPHGLDEEARNPTFSPNG
                     RYVAYTYRHDLYAKELSTGKVTRLTHDGSKTILNGLASWVYYEEILGRGGNYRSFWCS
                     PDSRRIAFFRSDDTNVPVFPIYVADGQHGYLEEARYPKAGDENPQVRVGIASVAADEG
                     IVWADIDNVADQYYGQPFWTPSGETLLVQWMNRDQDELKLFAVNPQDGAARMIYREKQ
                     PAWLEWISEMVFTQQGFLIVRDFESWQQIYLCSLDGSDFQRLTDGNNWGITLHGTDPK
                     EEFLFFSARREISTRTDVYRVPLPGKRNKDISVKRLTTGDCHAANILFAPDYRHFAVL
                     SSNVATPARSLLYSVEKGLVRVLGDSRGDAYDLELERGNVPVSEVHFITTEDGFTIPA
                     IITWPLNMEKGKKYPVIVNVYGGPDNGSVTDRWIAPASRYYWARKGVIQVTMDHRGSG
                     HCGKPGLDMMYRNLGKWEVHDYKLWMDYLRGLGCVDNNRVGITGYSYGGYVTALAVCT
                     AGDYFPYGIAGAGVYDWMFYDSHYTERFMDAPQDNPEGYRNGSVLAQCGTYGTWGPSV
                     LLVTHGTSDDNVHFQNAMRLADALMKTNKHFQFMIYPGARHGYRGYQGAYSAEEERAF
                     WTKYLLED"
     gene            4553..5767
                     /locus_tag="PLM68_04260"
     CDS             4553..5767
                     /locus_tag="PLM68_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT54610.1"
                     /translation="MITPGDIQFAREHLEDDIHGLLLGKHKYPGVDVPSCVIQIEGLR
                     KMRQKIPEWFSCRQIVVPSLLALEQASSQATALYKQRFCEGRGQVVDLTGGMGVDAYF
                     LALKSEGVVYCEKDTFLCQAARHNFNALGAGSRLQVVPGDAAGITGQPSILPYASLVY
                     ADPSRREDGKRLRGVENCSPDITQVRDIVLERAAAFLVKISPLADISRTLSQLPGTVE
                     VHVVSVGNECKELLFLMKRSDTGDAGPLIVCSAGFSFHRREETLAARAIEQNGFEGVP
                     VEGDCLHEPDVSILKAGAFALPVERFGVKKLHRNSHLYVSSCPVAGFPARKFRIREVI
                     PFTSANVKKMHTRFPAASITVRNFPLTVAELRKKTGIAEGGTGYLFGTTTCSKGQDAP
                     VIIYATKWEESI"
     gene            5764..7803
                     /locus_tag="PLM68_04265"
     CDS             5764..7803
                     /locus_tag="PLM68_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54611.1"
                     /translation="MKKTILFMVMTVLLGCSTLETKTEMSNITEQMVETTLARLGEAN
                     PEGLLPAAETGIRQTAGLWRTSDGDETDFMNFCLENYCTDEVAREALYDKLSNAFENL
                     FGTSNQLSVELKKPVHLEGGTLLPVDYILGNYDPSAHMMEDLFANKVAFICTLNFPNY
                     TLAEKDSLGREWDRKQWAYARMGDLFTHRTPAGLNQEMSQALGNADNYIASYNIVMGN
                     LLTEDGRRLFPEGMVLLSHWNLRDEIKSNYANVPDALEKQQMIHKVMEHIVCQTIPKC
                     VINNPEYDWKPYSNTVYKDGEPVEAEPESDDRYEHLLATFRVEKKMDPYHLNMPTAII
                     RNFEGSMEIQAAEIENLFINLISSEQVKKVAAFIRQRLGRELEPYDIWYDGFKSRTSF
                     PEDELTALTRRKYPNAEAFRSDMPRMLQALGFTTDKAASLASRIVVEPARGSGHAWGA
                     VGRWEPSRLRTRISPLGMDYKGYNIAVHEFGHNVEQTLSLYNVDHFMLSGVPNTAFTE
                     AMAFVFQKRDLQLLGYPPQQMDDNTVLDIFWGCYEIMGVALVDMYVWQWMYENPEATA
                     GALKEAVLEKARLVWNQYYEPVLGHHDSPLLAVYSHMISSPMYLPNYPFGHIIEFQLE
                     EYFNGKMKEEGKTLAQAITGMYTQGRLVPGLWMEQAVGSRVSTEPLLRAVDACDL"
     gene            complement(7775..8980)
                     /locus_tag="PLM68_04270"
     CDS             complement(7775..8980)
                     /locus_tag="PLM68_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOT54612.1"
                     /translation="MENSDILENLQKELHLELTTNILPYWEKLYDPHYGGYYGRVTGK
                     EELIPEAPKGAVLQSRILWTYSAAARILKDKRYLVPAARARDFVLDKLLDKEYGGVYW
                     STDHKGEPLDTRKQFYALGFAIYGLSEYHRATGDPRALDSAIGLFRDIETHSYDKIYG
                     GYLEAASRDWSEIRDFRLSEKDANEKKTMNTHLHILEPYTNLFRVWKDPGLEVALAGL
                     IRLFLDKIIDPSTHHLSLFFDEEYHVRGSAISYGHDIEASWLLMEAAKELADYKPGVY
                     GEILSRVTDQSRDVAFAALKGFQSDFSMIYEVASGGRIDKTRHWWVQAETVLGLFYLY
                     LWHKVPHALHMAVNCWNYIRENIVDKEHGEWFWSPGNTTDDKAGFWKCPYHNSRMCLQ
                     VIDHMHQLP"
     gene            complement(8967..10373)
                     /locus_tag="PLM68_04275"
     CDS             complement(8967..10373)
                     /locus_tag="PLM68_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54613.1"
                     /translation="MDKILFREKLGYGLGDMASSMFWKIFGMYLLFFYTKVFGISPAA
                     AGTMFLLTRIWDAVNDPMMGLIADRTHTRWGKYRPYILWGAVPFALIGIMTFYTPDWG
                     ENAKLAYAYVTYTFMMMVYTVVNVPYASLLGVMSPLPKDRNTLSSYRMFFAYIGSFIT
                     FMVLQPLVDAFSSGAQGGEQISTEPQAWTAAVAVIGGFCALLFFLCFRWTKERVVAAD
                     ESKNKASAGKDLKLLFSNKPWWILLASGVAALLFNSIRDGVALFYFSDYVTSTFRTAA
                     FGWTVGTIYLLVGQASNMIGVALAAPVANKIGKKSTYLVAMILAAILSCFFFLLKPDQ
                     IILIMVFQVMISTCAGIIFPLLWSMYADIVDYQELKTGRRATGLILSSSSMSQKFGWA
                     VGGAITGWLLAAFGYDAELASQSQGAIHGVRLMMSFLPAVGCILAILAVSFYPLSEKK
                     VMEITRQLNEKRAAHGKF"
     gene            complement(10382..>11015)
                     /locus_tag="PLM68_04280"
     CDS             complement(10382..>11015)
                     /locus_tag="PLM68_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOT54614.1"
                     /translation="QMGHLWRTTGDISAIFDGIEDHGTYKTLGVLQIIDLQDTLRRYA
                     GPDGWNDPDMLQVGNGMSESQDRAHFTMWCMLAAPLMAGNDLRTMSAATIEILMNKEV
                     IAIDQDPLGIQGFPYAKEGTVHTWLKPLSGGRWAVCFLNRGTQTVPLEIDWKKMVVKD
                     TLSGLELDPQANTYAVRDVWKKKDMGTARGKMKAPLPGHDVFCVILTPVN"
     assembly_gap    11016..11025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11026..11610)
                     /locus_tag="PLM68_04285"
     CDS             complement(<11026..11610)
                     /locus_tag="PLM68_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOT54615.1"
                     /translation="MKNKNRHFLLLLVALLSFLQAGAQKYEHMAPVPPMGWNSWNRFG
                     CNVDEKMLRETADMLVQTGMRDAGYVYLVIDDCWHGERDSLGFIHEHPDLFPSGMKAL
                     ADYIHARGLKFGIYSSAGFRTCAGRPASRGHEYQDALVYASWDVDYLKYDWCSTVGLN
                     AYGAYMTMRDALYKAGRPVVFSLCEWGNNQPWLWG"
     gene            complement(11607..>12573)
                     /locus_tag="PLM68_04290"
     CDS             complement(11607..>12573)
                     /locus_tag="PLM68_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HOT54616.1"
                     /translation="TLLTGLDYLLQELGKRDMLAVLFFNNTWEWTGGYSQYLEWAGYG
                     KAPVPVIDGWPAFMEYASQYVHCSLCDSLFRNHIRNIVTRVNSLTGVPYIEDPAIMSW
                     QIANEPRPMGAGNKEAYERFIVSTAQYIKSLDPNHLVSVGSEGSYGSENDMDLYRKVH
                     QTEFVDYLTCHIWPKNWMWIDIQDVPGSVERAAMLAEGYLEEHVSLAVALNKPLVLSE
                     FGFPRDNHRYDREDPVMARNAFYGRIFNRLAASAKASGNFAGCNIWAWGGFARPQHIF
                     WEPGDDYCGDPAQEEQGLNSVFAEDETVSLIQSYTTLLNEKQSQE"
BASE COUNT         3076 a         3082 c         3304 g         3101 t
ORIGIN      
        1 tctggcgtgc ctctacccat tggatcgggg ggatgggagt cgttcttttc atgttattga
       61 tcctgcccga ggtcagttcc atccgcctga aattatcccg gatcgagatc agctcattat
      121 ctcaggaatc ttaccatttc agggccaaag agtcagtgcg tgtgattgcc tatgtctatt
      181 ttggcttaac aatactggaa accattgccc tgtggatttc aggaatgaac ctctttgatg
      241 cagtttgcca ttcctttgcc accatagcca ccgggggatt cagcacccga aacaacagca
      301 ttgcctcata tgacagtgtt ttgatagaaa caatcattat tatctttatg accttgtctg
      361 cacttaattt tggaatgatt tttcttttag tggcaaaagg ttcattaatg ttgttcaaat
      421 ctcctgtaac cagatatttt ttatcagtat tattattagg aagcatactg gtatccctga
      481 atcttggact cacaaaaaca tatgactctt taggggaatc cttcagattc gggttttttc
      541 agacaatttc tttggcaaca tcaactggtt ttgcttctgc tgattcttca atgtggccaa
      601 attttgccat acttctcctg atctttttta cattgcaatg tgcttgttcc ggttcgactt
      661 caggaggtat taaagctgac aggatcctga tcgcattcta ttctataaaa gctcatatta
      721 caaaaagact tcatcccaga tcggtggttc caacaagggt gggagggcta gcgattgacc
      781 cggacacggt ttcaggagtg aacctattta ttgttttcta cttgttttgt gttttaattg
      841 cctcattatt attaactctt acaggagtgg atattatgga tgcagtaagt gctactgccg
      901 cccatatcgg aaatgtcggc ccgggttttg gcagtgtggg ttctatgagt aattacagtt
      961 ccttcaatag ttttgcaaaa tttgtcttat cggtgatcat gatcatgggg cgtattgaat
     1021 tgtttggatt tttgatattg ttcagttcgc gccggtaaag gattgatcat ggccattgac
     1081 cagtttatat ttgaaaagct ccgggaaaac ctaccttttg ccccatcctc cgatcaggat
     1141 gtctttttcc gcgccatcgc ctcttttctt agcggcccaa atcctgtttt catcctcaat
     1201 ggttatgccg gaacgggtaa aaccactgcc atggctgccc tggtgggtgc tctttcggaa
     1261 ttgaacaggc aggtagtcct ccttgcccca accggtcgtt cagccaaggt gctgatgggt
     1321 tatgccggtg ctcctgccca taccatccat aaggccatct accggcagaa accacctgca
     1381 tcatcggatg aaggcggatt cggagttttt gaattgcatc ccaaccgtga caaaaatacg
     1441 ctgttcattg ttgacgaggc ttctctgctg tccaacaccc ccgggggcat gctttttggc
     1501 agcggcaacc tgttggaaga cctggtcgct tatgtgttca acgggaaagg gaaccagctg
     1561 ctcatcctgg gtgactcggc acaactcccg cccataggac tggaactgtc accggcactg
     1621 gatcccgggt atatggaaca atacgggagc gtcgtctata tggagatgaa agaagtgctc
     1681 agacagtctt tcgattcggg catcctgtat aacgcaacgt tgttgcggga caacatcacg
     1741 gccggggatt attgcctgcc ggaatggaag acagcaggct ttaacgatgt gcgccgaata
     1801 gaatcttcgg aactgatgga atgtttgaat gatgccattt cgcgttatgg cctggaggaa
     1861 gtggtggtac tgtgccgctc caacaagagg gccaaccgat acaacaaggg aatacgaggc
     1921 agtatcttgt accgtgaaga ggggataact aaaggagaca gggtgatggt ggtaaagaat
     1981 tgttatcagt tcctggaaga tgtgccggaa atggatttca tagcaaacgg cgatatagcc
     2041 gagatcctca ggatctacaa attccatgaa cggtacgggt tccgttttgc agaagccgtc
     2101 ttgcgtttcc cggattataa agaaacggag atcagtgcgc gtctgctgct ggatacccta
     2161 gagagtgaat ctcccgcctt gtcccgggag cagcaggagc agctgtacca gggggtatac
     2221 agcgattatg agcacataaa agggaaacgc aaacgctaca atgcagtcag ggaggatttg
     2281 tacctgaatg cgttgcaggt gaaatatgcc aatgccgtta catgtcacaa ggcgcaggga
     2341 ggtcagtgga aggccgtatt tgtggataag gctttttttg gtgaaatttg tgataaagac
     2401 atgctgcgct ggtactacac cgcctttacc cgggccaggg aacaattgta tctgattaac
     2461 ctgtaatttg aaacatatga gacgttatgc aatttcttta atcctgctct tcctgttacc
     2521 cctggcaatc cgttcacagg aacgttacct ccttgaaaca ccaccgcaag gacttacaaa
     2581 gcccatagat acacacattt tctggaaaga caaccgtact ttgttgttgc gttccgggag
     2641 ccagcaggaa cctacttatt acctggaaga tattgtcagc gggaagcgtg tcaggacaga
     2701 tgatcctgct gatgttagta cagcaggaat accacacgga ctagatgaag aagccaggaa
     2761 tccaaccttt tctcccaatg gcaggtatgt agcctatacc tataggcatg acctgtacgc
     2821 caaggaatta tcaacaggca aggttacccg gctcacacac gatggcagca agaccatcct
     2881 gaacggcttg gcttcctggg tgtattacga agagatcctg ggccggggag gcaattaccg
     2941 ctctttctgg tgctcgccgg acagccggag aatcgcgttt ttccggagtg atgatacgaa
     3001 tgttcccgtg ttcccgattt atgttgccga tggacagcac ggatacctgg aagaagcaag
     3061 gtatcccaaa gcaggggatg aaaacccgca ggtacgcgtg ggtattgcat cggtcgctgc
     3121 ggatgagggt atagtctggg ctgatatcga caatgtcgcc gatcaatatt atgggcagcc
     3181 tttctggacg cccagcgggg agacgcttct ggtccaatgg atgaacaggg accaggatga
     3241 gctgaaactg tttgccgtca atccgcagga tggtgctgca aggatgatat atcgcgagaa
     3301 gcagcctgcc tggctggaat ggatttcaga aatggtattc actcaacaag gtttcctgat
     3361 agtacgtgat tttgaatcct ggcaacagat atacctttgt tctcttgatg gcagtgattt
     3421 ccaaaggctt acagacggaa acaattgggg gatcaccctt cacgggacag atccgaagga
     3481 agaattcttg tttttttctg ccagaaggga aatttctacc aggacagacg tctacagggt
     3541 acccctgccc gggaaaagga ataaggatat ttctgtcaaa cgtctcacca ccggggattg
     3601 ccatgcggcc aacatattgt ttgctcctga ttaccgtcat tttgccgtat tgtcatccaa
     3661 tgtggccaca cctgcccgtt cgctgctcta cagtgtggaa aaaggcttgg tccgggtatt
     3721 gggagattcc cgcggggacg cctatgacct ggagctggaa agaggtaatg ttccggtctc
     3781 ggaggtgcac tttataacta cggaagatgg ttttaccatt ccggccatta tcacctggcc
     3841 cctgaacatg gaaaaaggta aaaaataccc cgttattgtc aatgtatacg gtggacctga
     3901 caacggaagc gtgacagacc gctggattgc accggcatcc aggtactact gggcccgcaa
     3961 aggggtgatc caggtcacca tggaccaccg gggaagcggc cattgcggga agccgggcct
     4021 ggacatgatg tacaggaacc tgggcaaatg ggaggtacac gattacaaac tctggatgga
     4081 ttacctcagg ggacttggat gtgtcgacaa caaccgggtg ggtatcaccg gttacagtta
     4141 cggcggctac gtgacggccc tggccgtatg cacggcggga gattattttc cctatggcat
     4201 tgccggagct ggtgtatacg actggatgtt ctacgactca cattatacag aacggttcat
     4261 ggacgctccg caggacaatc cggaaggtta caggaacggt tcggtcctgg cacaatgcgg
     4321 gacctatggg acgtggggcc cttctgtctt gcttgtcaca catgggactt cagacgataa
     4381 cgtccatttt cagaatgcca tgcgcctggc agatgcgttg atgaaaacca acaaacattt
     4441 ccaattcatg atttacccgg gggccaggca cggttacagg ggttaccagg gtgcttattc
     4501 cgccgaggag gaaagggctt tctggacaaa gtacctgctg gaggattagc ctatgatcac
     4561 acccggtgat attcaatttg cacgagaaca cctggaggat gacattcacg ggctgttact
     4621 gggcaaacac aaatatcccg gggtggatgt tccgtcatgt gtcatccaga tagaggggct
     4681 gcgcaagatg cggcaaaaaa ttccggaatg gttttcctgc aggcagattg ttgttccctc
     4741 ccttttagca ttggagcagg cttcttccca ggcaacggct ctgtacaaac agcgtttttg
     4801 tgaaggcagg ggccaggtgg tggaccttac aggcggaatg ggagtggatg cgtattttct
     4861 tgcccttaaa agtgaaggag tggtctattg cgaaaaagac actttccttt gccaggcagc
     4921 ccggcacaat ttcaacgcac tcggtgcagg cagccgcctg caagtcgtcc cgggagatgc
     4981 cgccgggata acagggcaac cgtccatcct tccatatgca tccctggtct atgcggatcc
     5041 ttccagacgt gaggacggga aaaggttgcg cggtgtcgag aactgcagcc cggacatcac
     5101 acaggtccgg gatatcgtgc tggagcgcgc tgctgctttc ctggtaaaga tttctcccct
     5161 ggctgatatc tcccggacgc tttcccagct tcccggtacg gtggaggttc atgtggtatc
     5221 ggtggggaat gaatgcaagg aattgctctt tctgatgaaa agaagcgata ccggcgatgc
     5281 cggtcccctg atcgtttgct cggcaggttt ttcgtttcac agaagggaag agacgcttgc
     5341 tgcgcgggcc attgagcaga atgggtttga gggtgtgcct gtggaagggg attgtttgca
     5401 cgaaccggat gtctctatac tcaaagccgg tgcttttgct ctgcctgttg aaaggtttgg
     5461 cgtgaaaaag ctgcacagga acagccattt gtatgtatct tcgtgcccgg tagccggttt
     5521 tcctgcaaga aagttcagaa taagggaagt aatccccttt acctcggcca acgtgaaaaa
     5581 aatgcacacg cgctttcccg cggccagcat tacggtgcgc aatttcccgc tgaccgtggc
     5641 ggagttgaga aagaagacgg gaattgctga aggaggtacc ggttatcttt ttggaacaac
     5701 aacatgcagc aagggccagg atgcccctgt gattatatat gcaacaaaat gggaggaatc
     5761 aatatgaaaa aaacaatctt attcatggta atgactgtat tgctgggatg cagtacgtta
     5821 gaaacaaaaa cagaaatgtc taatattaca gaacaaatgg tagagaccac cctggcgcgt
     5881 ctgggggaag caaaccctga aggtcttttg cctgctgccg aaaccgggat acgtcagaca
     5941 gctggtttat ggaggacctc ggatggcgat gaaacggatt ttatgaattt ctgccttgaa
     6001 aattattgta cagacgaagt ggcccgtgaa gccctgtatg ataaattatc caatgcattt
     6061 gaaaatttgt tcggcacctc caaccagctg agcgttgaac tcaagaaacc agttcacctg
     6121 gaaggtggaa cgctgttgcc ggtagattat atactgggca actacgaccc ttctgcccat
     6181 atgatggaag acctgttcgc caacaaggtg gctttcatat gcacccttaa ttttcccaat
     6241 tacacgctcg cggagaagga ttccctgggc agggaatggg acaggaagca atgggcgtac
     6301 gccaggatgg gggacttgtt cacccaccgc accccggcgg gactgaacca ggaaatgtca
     6361 caggctttag gcaatgccga taattacata gcctcttaca acattgttat gggaaacctg
     6421 ctcacagaag atggcaggcg cctgtttcct gaaggtatgg tcttgttgtc ccattggaac
     6481 ctgcgggatg agatcaaatc aaactatgcc aatgtgccgg atgcgttgga aaaacagcaa
     6541 atgatccata aggttatgga acatattgtt tgtcagacca tcccgaagtg tgtgatcaat
     6601 aacccggaat acgactggaa accttattcg aataccgtat ataaagacgg agagcctgtt
     6661 gaggcagagc cggaaagcga tgaccgttac gagcacctgc tggcaacctt cagggtggaa
     6721 aagaaaatgg acccttatca tctgaacatg cccacggcta tcatccggaa ttttgaagga
     6781 tccatggaaa ttcaggcagc cgagatagaa aacctgttca tcaacctgat ttcctcggaa
     6841 caagtaaaaa aagtggcggc tttcatacgc caaaggctgg gccgtgaact ggaaccttat
     6901 gacatctggt atgatggttt caaaagcagg acatcctttc cggaagatga gctgacggcc
     6961 ctcacccgca ggaaatatcc aaatgcagaa gctttccgtt ccgatatgcc ccggatgctg
     7021 caggctcttg gttttacaac cgataaggct gccagcctgg catcacgcat tgtcgttgag
     7081 ccggcgcggg gatccggtca cgcctgggga gctgtcggac gctgggagcc gtcccggctc
     7141 agaacacgta tttcccctct tggtatggat tacaagggat acaacatagc cgtccatgag
     7201 ttcggccaca acgtagaaca aaccctgagc ctgtataacg tggatcattt tatgctcagc
     7261 ggtgtgccca acacggcctt caccgaggca atggcctttg tttttcaaaa acgcgacctg
     7321 caattgctgg gatacccgcc gcagcagatg gatgacaaca ccgtactgga tattttctgg
     7381 gggtgttacg agatcatggg ggtggctttg gtagacatgt atgtatggca gtggatgtat
     7441 gagaatcctg aagctacggc cggtgcgtta aaagaggcag tactggagaa agcgcggctg
     7501 gtgtggaacc aatactatga acctgttctg gggcatcacg attccccgtt gctggcggta
     7561 tattcacaca tgatcagcag tcctatgtat ctgcccaatt atccgttcgg gcacatcatt
     7621 gaattccagc tggaagaata cttcaacgga aaaatgaagg aagaaggcaa gacactggcc
     7681 caggcaatta caggcatgta tacacaaggc aggcttgttc cgggactttg gatggaacag
     7741 gctgtgggat cgcgtgttag tacagaaccg ttgctcaggg cagttgatgc atgtgatcta
     7801 tgacctggag gcacatccgg gagttgtggt aggggcattt ccagaatccg gccttatcgt
     7861 ctgtggtatt gcccggactc cagaaccatt ccccgtgttc cttgtccaca atgttctccc
     7921 tgatataatt ccagcaattg acggccatat gcagggcgtg gggaacctta tgccagagat
     7981 acaggtaaaa caaccccaga actgtttctg cctgtaccca ccagtggcgt gttttatcaa
     8041 tcctgccgcc tgaggccact tcgtaaatca tgctgaagtc ggattggaat ccctttagtg
     8101 ccgcaaaggc gacatccctg ctctgatctg taacccggct caggatttct ccgtataccc
     8161 cgggcttgta gtctgccagt tcttttgcag cctccatcag gagccaggat gcctcgatgt
     8221 cgtggccgta ggatattgcc gagccgcgaa catggtattc ctcgtcaaag aaaagggaca
     8281 ggtgatgcgt tgacggatct atgatcttgt ccaggaacag cctgataagc ccggccaggg
     8341 ccacttccag gccgggatcc ttccaaaccc ggaaaaggtt ggtatagggt tccaggatat
     8401 gcaggtgcgt gttcatggtc tttttttcat tcgcatcttt ttccgacagg cggaagtccc
     8461 ggatttcact ccaatcacgc gaggcagctt ccaggtatcc tccgtagatt ttatcgtaag
     8521 aatgggtctc aatgtcgcgg aaaagcccga tagcactgtc cagggcccgg ggatcccccg
     8581 ttgcccggtg gtattcactt agcccgtaaa tggcaaatcc aagagcatag aactgctttc
     8641 gggtatccag gggctcccct ttgtgatccg tgctccagta aaccccgccg tattccttgt
     8701 ccagaagctt atccagcaca aaatccctag ccctggcagc aggtacaagg tatcgtttat
     8761 ccttcaggat cctggcggcg gcagaatagg tccacaatat acgggattgc aacacggcac
     8821 ctttgggagc ctccgggatc agttcctctt ttcctgtcac ccgcccgtaa taccccccgt
     8881 agtgcgggtc atacagtttt tcccagtatg gcagtatgtt ggtggtgagc tccaagtgaa
     8941 gctccttttg caggttctcc agaatatcag aattttccat gagcagctct tttttcattc
     9001 agttgccggg tgatctccat gacttttttc tcactgagcg ggtaaaaaga taccgccagt
     9061 atggccagga tacaacctac ggcaggcagg aaactcatca tcagacgaac cccgtgaatg
     9121 gcaccctggc tttgggatgc aagctctgca tcatagccaa aggcagccag caaccagcct
     9181 gttatggcgc cgcctacggc ccagccgaat ttctgggaca tggacgaaga actgaggatc
     9241 agcccggtgg cacgccttcc ggttttcagc tcctggtagt ccacaatatc ggcatacata
     9301 ctccagagca acgggaaaat gatcccggca caagtgctaa tcattacctg gaagaccatt
     9361 ataaggatga tttgatccgg tttgagcagg aaaaagaagc aactcaggat ggcggccaga
     9421 atcatggcca caaggtaggt gcttttttta ccaattttgt tggccaccgg cgctgcaagt
     9481 gccaccccga tcatattgga agcctgtccc acaagcaggt atatggttcc cacggtccag
     9541 ccaaaggcag ctgttctgaa ggtggaggtt acgtaatccg agaaatagaa aagtgccacc
     9601 ccgtcccgta tggaattgaa aagcagggct gcaacgcccg aggcaagcag gatccaccag
     9661 ggtttgttgc taaagagcag tttaaggtct ttacccgccg aggctttgtt cttgctttca
     9721 tcggccgcca caacacgctc ttttgtccag cggaaacata aaaagaaaag cagggcgcaa
     9781 aagccgccga tcacggcaac ggcagcagtc caggcctgcg gctcggtgga tatctgctct
     9841 cctccctgcg ctccgctgct gaaggcgtcc accagcggct gcaggaccat aaatgtgata
     9901 aagctcccta tgtaggcaaa gaacatccgg tacgaggaca gggtatttct gtccttgggc
     9961 agaggggaca tgactcccag cagggaggcg tagggaacgt ttaccaccgt atagaccatc
    10021 atcatgaagg tataggtaac ataagcgtag gccagtttcg cattttcacc ccaatcgggg
    10081 gtataaaagg tcatgatgcc tatcaaggcg aagggaacgg ctccccataa aatataggga
    10141 cggtatttgc cccacctggt atgggtcctg tctgcaataa ggcccatcat gggatcattg
    10201 accgcatccc agatacgggt aagcaggaac atggtgccgg cggcagcggg tgagatgcca
    10261 aatactttgg tataaaagaa aaggaggtac attccaaaga tcttccagaa catgctggaa
    10321 gccatatctc ccaatccgta acccagtttt tcacgaaaca agattttatc catgagcagc
    10381 ctcaattaac cggtgtaagt ataacgcaga aaacatcgtg accgggaagc ggggctttca
    10441 ttttccccct ggctgttccc atgtcctttt tcttccacac atcccttacc gcataggtgt
    10501 ttgcctgcgg atcaagttcc agtccgctca gggtgtcttt aacaaccatt tttttccagt
    10561 caatttccag gggcaccgtc tgtgttcccc tgttcaggaa gcacacggcc caccggcccc
    10621 cggacaaagg tttgagccag gtgtgaacag tgccttcttt ggcataggga aatccctgga
    10681 ttcccagcgg atcctggtca atggctatga cttctttatt catgagtatt tcaatggtgg
    10741 ccgcactcat ggtgcgcagg tcgttccctg ccataagcgg ggctgccagc atgcaccaca
    10801 tggtgaaatg agccctgtcc tggctttcgc tcatgccatt gcccacttgt aacatgtcgg
    10861 gatcgttcca gccatcgggg ccggcgtaac ggcgcagggt gtcctgcagg tcgatgatct
    10921 gcagaacgcc cagggttttg tacgtgccat ggtcttcaat accatcaaat atggcagaaa
    10981 tatctcccgt tgtacgccac aggtgaccca tttgtnnnnn nnnnnacccc agagccaggg
    11041 ctggttgttg ccccattcgc acaggctgaa gacgacaggc cttccggctt tgtacagggc
    11101 atcccgcatg gtcatgtaag ctccgtaagc attgagcccg acggtgctgc accagtcgta
    11161 tttaagatag tccacatccc aggaggcata caccaaggca tcctggtact catgtccccg
    11221 gctggcaggt cttcctgcac aagtcctgaa tccggccgat gaatagatgc cgaatttcag
    11281 tcccctggca tgaatataat ctgccagggc tttcattccg gaggggaaca ggtcgggatg
    11341 ctcgtgtatg aatcccaggg aatccctttc cccgtgccag cagtcgtcaa tgaccaggta
    11401 gacatagccg gcatcgcgca ttcccgtttg gacaagcatg tcggccgttt ccctcagcat
    11461 tttctcatcc acgttacatc caaaacggtt ccaggagttc cagcccatag gggggaccgg
    11521 tgccatatgt tcatatttct gtgccccggc ctgcaagaag gacaacaagg caacgagcag
    11581 gagcaaaaag tgacgatttt tatttttcat tcctgggatt gtttttcgtt aagcagggtg
    11641 gtgtaggatt ggatcaggga taccgtttca tcttcggcaa aaacggaatt gagtccctgt
    11701 tcttcctggg cgggatctcc gcagtaatca tctccgggtt cccagaagat gtgttgcgga
    11761 cgtgcaaatc ctccccaggc ccagatgttg cagcctgcaa aatttcctga tgcctttgcc
    11821 gaagccgcca ggcggttgaa aatccggccg tagaatgcat ttctggccat tacgggatcc
    11881 tcccggtcat agcggtggtt gtcccgggga aatccgaatt cagataatac aagaggtttg
    11941 ttgagtgcaa cagccagtga tacatgttct tccaggtatc cttctgccag catggccgcg
    12001 cgttccactg aaccgggaac atcctggata tctatccaca tccagttctt gggccatata
    12061 tggcaggtca ggtagtccac gaattcagtc tggtgaacct tcctgtagag atccatgtcg
    12121 ttctcgctcc cataggaacc ttcagaacct acagaaacca aatggttggg atccagtgat
    12181 ttgatatact gggcggtcga aacgataaac cgttcgtagg cttccttgtt gcctgctccc
    12241 ataggcctgg gctcgttggc aatctgccag gacatgatgg ccggatcttc aatgtaagga
    12301 acaccggtca ggctgtttac acgggtgaca atatttctga tatggttcct gaacaggctg
    12361 tcacacaggc tgcagtgcac gtattgacta gcgtattcca tgaaagcggg ccatccgtcg
    12421 attacaggga ccggggcttt accgtaaccg gcccattcca ggtactggga atacccgccg
    12481 gtccactccc aggtgttgtt gaagaacaat acggccagca tatcccgttt gcccagttcc
    12541 tgcaaaagat aatccagtcc tgtaagcagg gtg
//
LOCUS       DAOSOF010000051        12485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5049_length_12485_cov_4.889702, whole genome shotgun sequence.
ACCESSION   DAOSOF010000051 DAOSOF010000000
VERSION     DAOSOF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_12485_cov_4.889702"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLM68_04295"
     CDS             <1..682
                     /locus_tag="PLM68_04295"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOT54617.1"
                     /translation="QDAFSFQSQQKAIRAIDAGKFRDEIVPVEIVSKKETLVFDTDEF
                     PNRATTLEKMANLRPAFKKDGTVTAANASGINDGASFVLLASEKAIKDHGLVPLAEIV
                     ATAQAGVDPAYMGMGPVPAIDKVLKRARMTLDQMDLVELNEAFAAQSLGVMLELQQQQ
                     GVDREWLDTHCNVNGGAIALGHPIGASGNRITVSLLYEMKRRKARYGLASLCIGGGMG
                     TALILKNV"
     gene            752..2398
                     /locus_tag="PLM68_04300"
     CDS             752..2398
                     /locus_tag="PLM68_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT54618.1"
                     /translation="MKTHYLEYIQNSIVTTWNMPGLTNYKKNSYTYGEIAQHVARHHI
                     LFEKLGLKKGDKVALCGPNSAEWGISFLAVTAYEAVAVPLLNDFLPESVMTLTDHSES
                     CVFFVSRDIWEKIDIAKMPHLKLVICLEGYELLYSKDPAVTQAHASIEVLFKEKYPGG
                     FGPEQVSYPTDNFDDLALINYTSGTTSAPKGVMLTYRNISSNIQFGQSEMPNGPGDTL
                     VSMLPMAHMYGMAFEYLYQLAGGTQVFFLGKVPSPKVLLDALATVKPYMMITVPLVIE
                     KIFKSSVFPTIQKPIIKSIMKVPGLASVIYKQVRKKLLHAFGGNIRTIIIGGAAINEE
                     VEDLMKAVKLPYTVGYGMTECGPLLSYEDTARFVKGSCGKPVQRMSLRIDSEDPRAIV
                     GEIQARGDNVMIGYYKNPEATAAAFTKDGWLRTGDLGLMDKDNNVFIKGRSKSLIIGA
                     SGQNIYPEEIEDKLNNQPYVVESIVVERDKKLVALVFPDYDRVSQESLDSMHLAQLME
                     ENRMRLNMLLPVFSRIAKIELQPEAFEKTPKRSIKRFLYK"
     gene            complement(2376..2957)
                     /locus_tag="PLM68_04305"
     CDS             complement(2376..2957)
                     /locus_tag="PLM68_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOT54619.1"
                     /translation="MKNDDKKLLLHACCGPCSTTALERLLQDYCVTVFFYNPNIAPHS
                     EYTLRRDEAKRFTELAYGGKVLFVEGRYNPDFFLKAVKGYEQEPEGGLRCKICYRLRL
                     EETARYAKENGFSLFCTTLTVSPHKNALLINKTGKDLAAQYNGLDYLPSDFKKRNGFR
                     RSVELSQLYHIYRQNYCGCQFSLSPSSICKGNV"
     gene            3137..4075
                     /locus_tag="PLM68_04310"
     CDS             3137..4075
                     /locus_tag="PLM68_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT54620.1"
                     /translation="MRVHTVSETTLGSEREYVGVLEESNNVLLSFPAPGKIKNVYVTE
                     GQAVREGQLLAQIDAGLLENMHATTAATLRQAEDAYQRMTLLYESQSLPEIQYVDIQT
                     KLEQARSAERISAMALAETKLIAPQRGVVGKRMMEPGMNVLPDQPVLTLLNTHNPVVK
                     VSVPENEIFDTRTGQEARIVVGALRNLELEGKVTEKGVQAHPYTHGYEVRISLDREVP
                     GLLPGMVCKVFLRNLPTEVALVVPVRAVLPLEFGKGHFVWLLDQDNVAQRRMVQVGEI
                     VPGGISVLKGITPGDRIVVEGYQRLSENIKVTVTNE"
     gene            4068..7190
                     /locus_tag="PLM68_04315"
     CDS             4068..7190
                     /locus_tag="PLM68_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT54621.1"
                     /translation="MSRKRRGVVEWTMHNHKIVLLLVAFLVGLGIFALPNMPKKEYPD
                     FTIRNALVVGVYPGASSEQVEEQLTIPLEKRILSYKEIDREKTYSLTREGMVYFVLDL
                     TEKEKSPRNFYANLRLDLDEFKAQLPPGVLPLMVMSEFGETSAILITLESESLTYDQL
                     DDYLDRLEGRLRPLPIVTNIKRYGLQQEQINIYLDKEKLAAYSLNTQQIMAQLFTSGL
                     VTSAGTVNTLNGSIPVHMNNAYQNENQILEQPVYFDPSGNVLRVKDIGRVSKEYQVPA
                     SYITNNGKKCLLLSMEMSKGFDMVKLGKQVDLVLKDFESELPPDVSVFRIADQPQVVK
                     DSVYSFLKEMLLAVLTVIAVMMVMLPLRVASVAAATIPITIFISLGMMYVLGFEINTV
                     TLASLLVVLGMVVDDSIVVIDNYMTLLDQGMSRWKASITAANSYFKSVFSATLAISIT
                     FFPFYFTFPENYVEFIFMAPWTISISLMVSLLVAMLFVPYFQYFTIRKGLKQVVEKQR
                     QKSKRGISILELFGVFYEKTLRKSFAHPKWVIAVIILFLVLPVFVYQQLPKRLMPMAD
                     RNQFVVEIYLPFGSSLGETEKLTDSLRRLMEQDERVLAITSFVGTTAPRFQASYTPQV
                     PGPHFGQFIVNTPSYKATENLVALFQEKYMHYFPGTYVRVRRLDYSLLSANEVEVRLK
                     GENKQAMMKLADSLATEFRKIPHIASVRSDFGGMQYGVGVHVDPIAASHVGINPGLLS
                     LNLASRYSGVPVTSIWSDGKTVPVLIRTDRQNETPSPDRLGDEYVQGIIPGAGVPLRQ
                     IAILEPEWNISQIVRRNGYPTLTLMMDLKYGKISTDVFDQVVEIMEDTPMPDGVWYEY
                     GGTYQADKSMMPGIIKGVAIAIFLIFLILLFHFNKVSLSLLVMGSTMLSILGAFAGTW
                     VLGLPVSLTTFLGLVTLIGLVVRNGIIMFDYAETLRGEGYSVKEAAFMAGCRRIRPIF
                     QTSSSTAVAVIPMLITNDSLWAPMAATIFFGVLTSMFFVSVFLPVIYWLFYRKQDRIK
                     SIREPEKLTL"
     gene            7190..8473
                     /locus_tag="PLM68_04320"
     CDS             7190..8473
                     /locus_tag="PLM68_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT54622.1"
                     /translation="MRKTIYLIITLCFPAMLQPLTAQPYTLEACRQMTLENNALLKEM
                     KIGEEISRQVKKQALTYFFPNISAFGMGFQASNPIISLDLGMLGSMALLKDGLLVSAM
                     AVQPVFMGGKIFYSTKLADTGIHASELFTVQKEDEVLAKTEEYYWLLYSLYEKRKTVA
                     IMQQVLDSLYKDVQQAVNAGVLGQNEVLKVKLQQHTLSTKNVELENGIFLANMALARQ
                     MGLPVDSLDNFMITFPADFQPEDPMQYYLDPSSVLSLRTEYKLLEMGVRAAALEKKIE
                     TASYMPQVGVGAFYYYENLLDKNNWNGAVMAAVRVPISDWSRGSFAIRQRKLEEEKAQ
                     IRKEDGEQQLLMQIRQAWGSFTESYQKYLLAELTVEESQENARLNETFYRAGTVSLTE
                     LLAARGFLQQSQDQRIDAIKDYQVAKSIYLQVTGR"
     gene            complement(8480..10168)
                     /locus_tag="PLM68_04325"
     CDS             complement(8480..10168)
                     /locus_tag="PLM68_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54623.1"
                     /translation="MKRILTVLLLSASSLLLQAQPPMLHERLYLVTDKESYLAGEHIW
                     ISCFCYDAVMGKPSLVSAVAYLELQNLSGSVVQAKIALAKGRGSGLIALPVSLPTGNY
                     RLIAYTRYMYSESEQNYFSRIVTVYNPLTSLRSDKVRLASGTGPVHYPGLTQENTREI
                     SVLTDRKAYESGEEVRITLSNHMPGEVSLSVSVFRKDNLNHYRNPSISSVVDSTRNQV
                     FTPFPLWRVDYPGEIIRGHVVDENNNPVAPKGGFGSYISIAGDQVQYYTGEIRENGSV
                     RFFTSNLYGNGTLVSHVPSLNETKYHLVLDSVYMDPPVEELPELVLDKNQQPVLKERS
                     LGIQLSQAFRLDTLFREEKAETNLLFENSGIVYKLDEYTRFPTMGEVMVEFIREARFR
                     TIGGTRVLQLRLRDAAGVAFYVDDPTPPLVLLDGIPVMDHEKIYSYDPALVREIVIYP
                     DKYAFGPIYYNGILFLKTYRGDFPDLELEGSMRIHEFQGVQNHYAFGVVSPGSRLPDL
                     RHTMYWNPRVDLKDEEPVTLRTTTPQTKGTFVVVAEGMSLDGKAFRTSAEFVVK"
     gene            complement(10180..11355)
                     /locus_tag="PLM68_04330"
     CDS             complement(10180..11355)
                     /locus_tag="PLM68_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOT54624.1"
                     /translation="MKYLLKLFGIVFPALIWVSCIVPYEPEELVNLDDIIVVEGNISL
                     TGPFSIRITRSAPISTYQYGQRTIKIERNARVFVRDSKGQTYKADTVTAYGEYLFDFS
                     GLRPSLEEEYQLVIESQDGQTYETDFRKSRHSSDMELDYIIDTLEQKIRIFVSSHDDA
                     GMSKYYYWNYTETWDYVSQMPAYCYFDVSKVKETIASRPWMYRCWNSRGSSEILLVET
                     SSLVQDRIIDLEIHSIDFRDIRITQHYSIEVVQTVLDNDAYLYMENMRKNSYDIGDIF
                     SPQPNEIRGNISCVTDPSVRAIGFVSVCNSVRKRLFISCIGLPGYVREPMPPDTVVPL
                     NSTVGFMIALIQQGFSPHTIDPLAGVSYWNLTRCIDCRSKGGKPEVPSFWPAEWPRF"
     gene            complement(11367..>12485)
                     /locus_tag="PLM68_04335"
     CDS             complement(11367..>12485)
                     /locus_tag="PLM68_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54625.1"
                     /translation="FDDRSLVSSDILQREQAFETALYLSDNWDITPELSVEMGIRYGL
                     YSALGPNRHYLYDPGKPRSEETATDSVYVGAGKMIAPYHGPEFRLSMRYAFTSDFSVK
                     AGFNSMRQNIHMLSNTTVISPTDIWKLSDANIRPQKGWQTALGFYKNLMNNTLELSVE
                     GYYKHMDRYLDYASFARLLMNPRIETDVIETRGRAYGVEIMLKRSLGRLNGWISYTYS
                     RTQLKQDDPRVAEPVNKGNWYPAPYDKPHDLKLVGNFKFTHRFSLSMNLDYSTGRPIT
                     MPIGTYHYAGGWRLLYGDRNAHRIPDYFRLDISFNIEPSHKLTLLTHSSFTIGVYNVT
                     GRKNPYSVFFRTEGITVNSYMLSIFGSPIPYITYNIRF"
BASE COUNT         3247 a         2911 c         3078 g         3249 t
ORIGIN      
        1 gcaggatgcc ttttctttcc aatcgcagca gaaggccata cgggccatag atgccggaaa
       61 gttcagggac gagatagttc ccgtcgaaat tgtcagcaag aaagaaactc ttgtttttga
      121 tacggatgaa ttccccaaca gggccacaac actggaaaag atggctaacc tgcgtcccgc
      181 tttcaaaaaa gacggaacag tcacggctgc aaatgcctcg gggatcaatg acggggcttc
      241 gtttgtattg cttgcctcgg aaaaggcaat taaggaccat ggcctggttc cgctggcaga
      301 aatcgtggct acggcacagg caggtgttga tccggcttat atgggtatgg gccccgtacc
      361 ggctattgac aaggtgctta aaagggcccg gatgacgctg gatcagatgg accttgtaga
      421 actaaacgag gcttttgccg cccaatccct tggcgttatg cttgagctgc aacaacagca
      481 aggtgtggac cgtgaatggc ttgacacaca ttgcaatgta aacgggggag ccatagccct
      541 ggggcatccc ataggcgcat ccgggaaccg cataacagtt tccctgctct atgaaatgaa
      601 gcgcagaaaa gcccgctacg gacttgcttc tttgtgcata ggaggcggaa tgggaacggc
      661 actgattctg aagaacgtct gaaatacctg ttaggaaaaa ggattatttc aaaaaaatac
      721 gtatttttaa ccagtattaa aaaaactggt tatgaaaact cattatctgg aatatatcca
      781 aaacagtatt gtcacgacat ggaacatgcc cggtctgacc aattacaaga agaactccta
      841 tacgtatggc gagatagccc aacatgttgc ccggcaccat atcctgtttg aaaagctggg
      901 attgaaaaaa ggggacaaag tagcgctgtg cggacccaac agtgcagaat ggggcatatc
      961 gtttctggca gttactgctt atgaagcagt cgctgtgccg ttgcttaacg atttcctgcc
     1021 cgaaagcgtc atgacactga cagatcattc agagtcctgt gtcttttttg tgagccggga
     1081 tatctgggag aagatcgata tagctaagat gccacatctt aaacttgtaa tttgtctgga
     1141 agggtatgaa ctcctttatt ccaaagatcc tgccgtgact caggcccatg cctcgataga
     1201 ggtcctgttc aaagaaaaat atcccggcgg atttgggccc gaacaggtca gttatccaac
     1261 cgacaatttt gatgatctgg cgctgatcaa ctacacttca ggcaccacca gtgcccctaa
     1321 aggagtcatg ctgacctaca gaaatatcag ttccaatatc cagttcggac aaagtgaaat
     1381 gcccaacgga cctggtgaca cgctggtttc catgctgcct atggctcata tgtatgggat
     1441 ggcctttgaa tacctttacc agctggccgg aggaactcag gtctttttcc tgggaaaagt
     1501 accttctccc aaggtgctgc tggatgccct ggcaacggtc aagccctata tgatgattac
     1561 cgtacccctt gtgatagaaa aaatcttcaa gagttcggtt tttcccacca tccagaaacc
     1621 aattatcaaa tccatcatga aagtacccgg gctggcttct gtgatataca aacaggtccg
     1681 caaaaaacta ttgcatgcct ttggcggcaa tattcgtacc atcataattg gaggggcggc
     1741 catcaacgag gaagtggaag atctgatgaa agccgtgaaa ctgccttata ccgtaggtta
     1801 cggaatgacc gaatgcgggc cgctgctttc ctatgaagat acggccagat ttgtcaaagg
     1861 ttcctgcgga aaacccgtac aacggatgtc gctcagaatt gattctgagg atccacgtgc
     1921 cattgtagga gaaatccagg cccggggaga caacgttatg ataggttatt acaaaaatcc
     1981 cgaggcaacg gcagctgcct tcaccaaaga cggatggttg cgtaccggtg atcttgggct
     2041 tatggataag gacaataatg tgtttatcaa gggtcgcagc aagagcctga ttattggggc
     2101 aagcggccag aatatctatc ccgaggagat cgaagacaag ttgaacaacc agccctacgt
     2161 ggtggaatcc attgttgtgg agcgcgataa aaaactggta gccctggtat ttcccgatta
     2221 tgaccgggtg tcccaggaat cgctggattc aatgcacctg gcacaattaa tggaagaaaa
     2281 ccgtatgaga ctcaatatgc tgttgcctgt tttcagccgt attgccaaaa ttgaattaca
     2341 gcccgaagct tttgaaaaga cacccaaacg cagtatcaaa cgtttccttt acaaatagag
     2401 gagggggata aagagaactg gcaaccgcag taattctggc ggtaaatatg gtacaattgt
     2461 gatagctcaa cggaacggcg gaaaccgttc cgttttttaa aatctgaagg caggtagtca
     2521 agtccgttgt attgagccgc cagatctttc cctgttttat tgatcagtaa agcgtttttg
     2581 tgaggactga cggtcagggt ggtacaaaaa agtgagaacc cgttttcttt tgcataccgg
     2641 gctgtttctt ccagcctgag cctgtagcat attttacagc ggagtccgcc ttcgggttcc
     2701 tgttcatatc ccttaacagc tttcagaaaa aaatcggggt tgtatcggcc ttccacaaaa
     2761 agaacttttc caccataagc cagttccgta aatcttttcg cttcatccct gcggagggta
     2821 tattcactat gcggtgcaat atttggatta tagaaaaaaa cagtaacgca gtaatcctgt
     2881 aacaagcgtt ccagggctgt tgttgaacag ggaccgcaac aggcatgaag caggagtttt
     2941 ttatcgtcgt ttttcataga tatttggttg caaatataat ggttttattg attttgatta
     3001 cttttgcatt tattaaccaa tctgttatgg aattgttcaa aaatctgtta ttctaattgt
     3061 ttttagcctt ctggcactcc tgtcttcgtg taggtcaaag ccggaaaagc acgagcaatt
     3121 atcactcccg gtaaatgtgc gtgttcatac tgtttccgaa accactttag gatcggaaag
     3181 ggagtatgta ggtgtgttgg aagaatcgaa caatgtcttg ttgagctttc cggctccggg
     3241 gaaaattaaa aacgtttatg tcacagaagg gcaggcggtc cgggaaggac aattacttgc
     3301 ccagatcgat gccgggctgc tggagaacat gcatgccact acggcagcca cgctcagaca
     3361 ggcagaagat gcctaccaga gaatgacact gctgtatgaa tcccagagcc ttccggaaat
     3421 acaatacgtc gatatccaga caaagcttga acaggccagg tctgccgagc gcatatcggc
     3481 aatggccctg gccgaaacca aactcattgc cccacaacgc ggggtggtgg gaaaacgcat
     3541 gatggagccc ggaatgaacg tattgcctga tcagccggtg ctgactttat tgaatacgca
     3601 caaccccgtg gtcaaggttt ctgttcctga aaatgagata tttgacacca gaaccggaca
     3661 ggaggcccgc attgtggtag gagcactccg gaatctggaa ctggagggaa aggtaacaga
     3721 aaaaggcgtg caggcacacc cttataccca tggttacgaa gtcaggattt ctcttgaccg
     3781 ggaagttccg gggttgcttc cgggcatggt ctgcaaggtt tttctacgta acctgcccac
     3841 ggaggttgcc cttgtggtgc cggttcgcgc cgtgcttccc ctggaattcg gcaaaggtca
     3901 ttttgtttgg ctcctggacc aggataatgt agcccaaaga aggatggtcc aggtgggtga
     3961 gattgtgcct ggggggatct cggtcctgaa aggaattact cccggagaca ggattgtggt
     4021 ggaaggatat cagagattga gcgaaaacat taaggtaacc gttacaaatg agtagaaagc
     4081 gaagaggcgt tgtcgagtgg acgatgcaca accataagat cgttttgctg ctggttgctt
     4141 tccttgtcgg cctgggtatc tttgctttgc ccaatatgcc taaaaaggaa tatcccgatt
     4201 ttaccatccg caatgccctg gttgtgggtg tatacccggg agcttcatcg gagcaggtgg
     4261 aagaacaact taccatccca ctggaaaaac gtatcctcag ctataaagag atcgatcgtg
     4321 aaaagactta ctccctgacc cgtgaaggga tggtctactt tgtcctggat cttacagaaa
     4381 aggagaaatc gccacgcaat ttctatgcga acctccgcct cgacctggat gaattcaaag
     4441 cccagctgcc acccggagtt ttgccgctga tggtcatgag tgagtttgga gaaacctcgg
     4501 ccattcttat tacccttgaa agcgaatccc tgacatacga ccagttggat gattatcttg
     4561 acaggctgga agggcgcctc aggccgctgc ccatagtaac caatatcaaa cgttatggcc
     4621 ttcagcagga acagattaac atttacctgg acaaagagaa actggctgcc tattctttga
     4681 atacgcaaca gattatggcg caactcttta cttcaggcct ggttacttct gccggaacgg
     4741 tcaatacact caacgggagc ataccggtcc acatgaacaa tgcctatcag aacgaaaatc
     4801 aaatcctgga acagccggtt tactttgatc cttcgggcaa tgtccttcgt gtcaaggata
     4861 ttggacgtgt cagcaaagaa tatcaggttc ccgcttctta tataacaaac aacgggaaaa
     4921 aatgcctgct cctgtccatg gaaatgagca agggatttga tatggtgaaa ttgggaaaac
     4981 aggtggacct ggtattgaag gactttgaat ctgaacttcc tcctgatgta agcgttttcc
     5041 gaattgcaga ccagccccag gtggtgaagg attcggttta ttcattccta aaggaaatgc
     5101 ttctggccgt tttgacagtc attgctgtca tgatggtaat gcttccactg cgtgtggctt
     5161 ctgttgccgc agcaaccatt cccataacca tattcatatc cttgggaatg atgtatgtcc
     5221 ttggtttcga gatcaatacc gtgactctgg cttcccttct ggttgtcctg ggcatggtcg
     5281 tggatgacag catagtggtc atagacaatt atatgaccct gctggaccag ggcatgagtc
     5341 gttggaaagc ttccattact gccgccaatt cctactttaa atccgtcttt tcggctacac
     5401 tggctatcag tataaccttt ttcccctttt atttcacatt tcccgaaaat tatgtagagt
     5461 tcatctttat ggcgccctgg actatttcca tttccctgat ggtttctttg ttggttgcca
     5521 tgctgttcgt gccttatttc cagtacttta ctatccgaaa aggattgaaa caggtggtgg
     5581 aaaaacaacg gcaaaaaagt aaaagaggaa tttcaatcct ggaattattc ggagtattct
     5641 atgaaaagac tttacgcaaa tcctttgccc atcccaaatg ggtgatagca gtcatcatac
     5701 ttttcctggt attgccggtg tttgtatacc agcaattacc caagcgcctg atgcccatgg
     5761 cagacagaaa tcaattcgtg gttgagattt acctgccgtt cggttcttct ttaggtgaaa
     5821 cagaaaagct gacagattcc ctgcgcaggc tgatggagca ggatgagcgg gtattggcta
     5881 tcacctcttt tgtaggaacc actgctcccc gttttcaggc ttcctacacg cctcaggtcc
     5941 ccgggccaca cttcggacaa tttattgtta acacaccatc atacaaggcc acagaaaacc
     6001 ttgttgcctt atttcaggaa aagtacatgc attatttccc cggcacctat gtccgggtaa
     6061 gacgcctgga ttattccctc ctttcggcca atgaagtaga agtgaggctc aagggagaga
     6121 acaaacaggc catgatgaag ctggcagatt cacttgcgac tgaattcagg aaaatccccc
     6181 atatagccag tgtccgttcc gatttcgggg gaatgcaata tggtgtggga gtgcatgtag
     6241 atcccatagc ggcatcccac gtgggtatca atccgggatt actgtcactt aacctggcat
     6301 ccaggtattc cggggtacct gtgacttcca tatggtcaga cggaaaaacg gttcctgtgc
     6361 tgatccggac agacagacaa aacgaaacgc cttcccctga taggctggga gacgaatacg
     6421 tacagggaat catcccgggt gcgggtgttc cattgcgcca gattgccata ctggaaccgg
     6481 aatggaacat atcccagatt gtccgcagga acgggtatcc aacactcacc ctcatgatgg
     6541 acctgaaata cggaaaaata tccaccgacg tgtttgacca ggtagttgag attatggagg
     6601 atacacccat gcccgatggt gtctggtacg aatacggcgg cacctaccag gccgataaat
     6661 ccatgatgcc cggaataata aaaggggtgg ctattgccat tttcctgatc tttctgatcc
     6721 tgctttttca cttcaataaa gtctcccttt ccttactggt catgggatct actatgctta
     6781 gcattctggg ggcctttgcc ggaacctggg tactcggtct gccggtaagc ctgaccactt
     6841 tcctgggatt ggtaaccttg attggactgg tggttcgcaa cggcattatc atgttcgatt
     6901 acgccgagac gctccgcggg gaaggctatt ctgtaaagga agcggcattt atggcagggt
     6961 gcaggcgcat ccggcccata ttccagacat cttcctctac agctgtcgca gtcatcccta
     7021 tgctgatcac caacgattcg ctctgggcac ccatggccgc caccatcttt tttggtgttc
     7081 tcacttcaat gttctttgtg tctgtttttc tgccagttat ttattggctc ttttacagga
     7141 aacaggacag gatcaaatct attcgtgaac ctgaaaaact gacattataa tgaggaagac
     7201 catttacctg ataattacgc tctgttttcc tgccatgctt cagcctctga cagcccagcc
     7261 ttataccctg gaagcatgca ggcagatgac ccttgaaaat aatgcgctcc taaaggagat
     7321 gaagataggg gaagaaatat cacgccaggt gaaaaaacaa gcccttacat acttttttcc
     7381 caatatttcc gcctttggaa tgggattcca ggcaagcaat cccatcatat cgctggatct
     7441 gggaatgctc ggcagcatgg ctttgcttaa ggacggatta ctggtcagcg caatggcagt
     7501 tcagcctgtt ttcatgggag ggaagatctt ttattcgaca aagcttgccg atacgggcat
     7561 tcatgccagc gaactattca cagtacagaa agaggatgag gtgctggcca agacagaaga
     7621 gtactactgg ttgttgtatt cattgtacga gaaaagaaaa accgttgcca taatgcagca
     7681 agtcctggat tccctttaca aagacgtgca acaggccgtt aatgcaggtg tcctgggaca
     7741 aaacgaagtg ttgaaggtta aattgcagca acataccctg agtacaaaaa atgtggaact
     7801 cgagaacggt attttcctgg ccaacatggc cctggcacgt cagatgggtt tgcccgtaga
     7861 ttcactggac aattttatga ttacttttcc tgctgacttt cagcctgaag atcccatgca
     7921 atattacttg gatccgtctt ctgtgctttc gctcaggaca gaatacaagc tcctggaaat
     7981 gggagtccgt gccgccgcac tggaaaaaaa gatcgaaact gcttcctata tgccccaggt
     8041 aggggttgga gccttttatt attatgaaaa cctgctagac aaaaacaact ggaacggagc
     8101 cgtcatggca gccgttcgtg ttcccatatc agactggtcc aggggttctt ttgccatccg
     8161 tcagaggaag cttgaggagg aaaaagcaca aattcgcaag gaagacggtg agcaacaatt
     8221 gctgatgcaa atccgccagg cctggggctc tttcacagag tcataccaga aatacctact
     8281 ggcagaactt actgttgaag aatcgcagga aaatgctcgt ttaaacgaga ctttttacag
     8341 ggcaggtacg gttagcctga ccgaattgct ggccgcccgg ggttttctgc agcaaagcca
     8401 ggatcaaagg attgatgcca taaaggatta ccaggttgca aaaagcatat acctgcaggt
     8461 caccggacgc tgaaataatt tatttgacaa caaattctgc agaagtcctg aaggcttttc
     8521 catcaaggct cattccctcg gcaacaacta caaaagtgcc cttcgtttga ggggttgttg
     8581 tcctgagtgt gacaggttct tcatctttca ggtctacccg gggattccag tacatggtat
     8641 ggcgcaggtc cggcaaacgg ctgccgggtg ataccacccc gaaagcatag tgattctgta
     8701 ccccctggaa ttcatgtatc ctcatggatc cttccagttc caggtcagga aagtctcccc
     8761 ggtaagtttt caggaatagt atcccgttgt aatatatcgg gccgaaggca tatttatcgg
     8821 gatagatcac tatctccctt acaagggcgg gatcgtatga gtatatcttt tcgtggtcca
     8881 taaccggaat gccgtccaat agcaccaggg gaggggtggg atcgtccacg taaaaggcaa
     8941 ctcccgctgc atctctcaat cgcaactgca gcacacgggt accgccgata gtcctgaaac
     9001 gggcttctct gatgaattcc accatgactt ctcccatggt cgggaaacga gtgtactcgt
     9061 ccagcttgta tacgattccc gagttttcga acaacaggtt agtctcggct ttttcttccc
     9121 tgaacaatgt gtccagccgg aaggcctgcg acagctggat acccaggctt ctttccttca
     9181 gtacgggctg ttggttctta tcaaggacca gctctggaag ctcttcaacc gggggatcca
     9241 tgtagactga atccagcacc aggtgatact ttgtttcgtt caatgaagga acgtgactta
     9301 ccaatgtccc gttcccatat aggttagagg tgaaaaaacg cacgctgcca ttctccctta
     9361 tctctccggt ataatactgg acctggtctc ccgcaattga aatatacgat ccaaaacccc
     9421 ctttgggtgc tacagggttg ttgttttcgt ccactacatg tccgcggatg atctctcccg
     9481 ggtaatcaac cctccacagg gggaaagggg taaatacctg gttccttgta ctgtcaacta
     9541 cagatgatat ggatggattc ctgtaatggt tcaaattgtc ttttctgaaa actgaaactg
     9601 atagcgatac ttctccaggc atgtgattgc tgagcgtaat cctgacctct tcaccggatt
     9661 cgtaagcttt tctgtcagtc aacacgctga tttcccgtgt attttcctgt gtcagtccgg
     9721 ggtaatgtac aggtcctgtt ccggaagcaa gccttacctt gtcggaacgt agtgatgtaa
     9781 gcgggttgta aactgtaacg atcctggaaa aataattctg ttcactttcc gaatacatgt
     9841 accgtgtata ggctatcaac cggtaatttc cggtgggaag ggaaacaggc agggcaatca
     9901 gaccgcttcc ccggcctttt gcaagggcga tctttgcctg taccactgaa ccggaaagat
     9961 tttgaagctc caggtaggca actgcgctca caagagatgg ttttcccatc acggcatcat
    10021 agcaaaagca ggatatccag atgtgttctc ctgcaagata actctctttg tccgtgacca
    10081 ggtacaggcg ttcatgcagc atagggggct gtgcctgtaa taacagggaa gatgcagata
    10141 gtaaaagtac tgtaagaatc cgtttcatga gtacaacttt taaaatctag gccattcggc
    10201 aggccagaac gatggaacct ccggttttcc gcctttgctc cggcagtcga tacatctggt
    10261 aaggttccag tagctaacac cggccagggg gtcaatggtg tggggggaaa atccctgctg
    10321 gataagagca atcatgaagc ctactgtcga attaagaggc acaacagtat ctggcggcat
    10381 gggttcgcgg acatatccag gtagtcctat gcagctgata aacagccttt ttcttacaga
    10441 gttgcagaca gacacaaatc ctatggcacg tactgaagga tcggtcacgc agcttatatt
    10501 acctctgatc tcgtttggct ggggcgaaaa aatatcacca atgtcgtatg agttcttacg
    10561 catgttttcc atatacagat aggcatcatt gtccagaact gtctggacca cctctatgga
    10621 ataatgctgg gttatccgga tgtcgcgaaa atcgattgaa tggatctcca ggtcgattat
    10681 cctgtcctgg accagtgagg aagtctctac gagtagaatt tcagaggatc cacgactgtt
    10741 ccagcagcga tacatccacg gtctggaagc tattgtttcc ttaactttgg agacgtcaaa
    10801 ataacagtat gccggcatct gtgacacgta atcccatgtt tccgtgtagt tccagtaata
    10861 atacttggac atcccggcat catcatgtga actgacgaat atgcgaattt tttgttccag
    10921 cgtatcaatg atgtaatcca gttccatatc gctgctgtgc cgggatttcc ggaaatcagt
    10981 ctcgtaggtt tgcccgtcct gtgactctat caccagctgg tattcttctt ccaggcttgg
    11041 ccgcagtccg gaaaaatcaa aaagatattc accataggcg gtgacggtat ctgccttgta
    11101 tgtctgtccc ttgctgtctc ggacgaaaac cctggcattg cgctcaattt taatggttct
    11161 ctgaccgtac tgataggtgc ttatgggggc actcctggtg atacggatac tgaagggacc
    11221 ggtcagcgat atgttccctt ccaccacaat gatatcatcc aggttgacca gttcttccgg
    11281 ctcatagggt acgatacagg acacccagat cagtgccgga aacactatcc caaaaagctt
    11341 caataggtat ttcataggca ttgagattaa aatctgatgt tgtatgtgat gtagggaatt
    11401 ggacttccaa aaatggaaag catgtaggaa ttgaccgtga tgccttccgt gcggaagaat
    11461 accgaatacg gattctttct tccggtcacg ttatacacgc caattgtgaa agagctgtgg
    11521 gtcagcaggg taagcttgtg agaaggctca atgttaaaag atatgtccag cctgaagtaa
    11581 tccggaatcc ggtgggcgtt gcggtctccg tataatagcc gccagcctcc cgcatagtgg
    11641 taagtgccta tcggcatggt tatgggcctt ccggtgctgt agtccaggtt catggacaag
    11701 ctgaaacggt gtgtgaattt gaaattccca accagtttca aatcatgtgg cttgtcgtag
    11761 ggtgcagggt accagttccc cttgttcacg ggttctgcca cccttggatc atcctgtttg
    11821 agctgagtcc gcgaataggt gtaagagatc cacccgttga gccttcccag tgatcgcttg
    11881 agcattatct ccactccgta tgcccttccc cttgtttcaa taacatctgt ctctatgcgg
    11941 ggattcatca gcaggcgggc aaaggaggcg taatccaggt agcggtccat atgtttgtag
    12001 taaccttcta ctgatagctc cagggtgttg ttcataaggt ttttataaaa accgagagcc
    12061 gtttgccaac ccttctgggg cctgatgttg gcatcggata atttccagat atcagtaggg
    12121 gatatcacgg tagtgttgga caacatgtgg atgttctggc gcatgctgtt aaagcctgcc
    12181 tttacagaga aatcgctagt aaaggcatag cgcatggaaa gcctgaactc gggtccatga
    12241 taaggggcga tcatttttcc ggctcctaca tagacgctgt ctgtagcggt ttcctccgac
    12301 ctgggtttcc cgggatcgta caggtaatga cggttgggcc ccagtgcaga atagagaccg
    12361 taacggattc ccatttctac agacagttca ggtgtgatat cccagttatc gctcaggtat
    12421 agagctgttt caaaggcttg ttcccgttga aggatgtctg aagaaaccag ggacctgtcg
    12481 tcaaa
//
LOCUS       DAOSOF010000052        12330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5125_length_12330_cov_4.704766, whole genome shotgun sequence.
ACCESSION   DAOSOF010000052 DAOSOF010000000
VERSION     DAOSOF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_12330_cov_4.704766"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1070
                     /gene="gap"
                     /locus_tag="PLM68_04340"
     CDS             66..1070
                     /gene="gap"
                     /locus_tag="PLM68_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT54626.1"
                     /translation="MNKIKVGINGFGRIGRLVFRAAQDFGNIEIVGINDLIDVDYIAY
                     MLRYDTVYGQFQGSIEVRDNKLVVNGKSIRVTAEKDPINLKWNEIGAEYVVESTGLFL
                     TKEKAQGHLAAGAKRVIMSAPSKDDTPMFVFGVNHKLYKGEQIISNASCTTNCLAPLA
                     KVLNDKFGIVEGLMTTVHATTATQKTVDGPSAKDWRGGRAAAANIIPSSTGAAKAVGK
                     VIPQLNGKLTGMSFRVPTVNVSVVDLTCRIEKPASYDEIKAAMKEASEGEMKGVLGYT
                     EDAVVSSDFLHDSRTSIFDAGAGISLNPNFVKVVSWYDNEWGYSCKIVDMIQHMATVK
                     "
     gene            1137..2141
                     /locus_tag="PLM68_04345"
     CDS             1137..2141
                     /locus_tag="PLM68_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT54627.1"
                     /translation="MVSVIMAAFNAQAYIEAALDSVFAQTFRDFEIVVVDDASTDGTG
                     HILENRKDPCLRVFRNETRSGAAFSRNRAIAEASGKYMAVLDADDFWAPQKLEKQVEY
                     MEKHPAAAGLGTYVYETDHTGHPVKAVRFPLYPGQIRCSTLFRCSLVHSSVMVRRSFL
                     VEQNLRYDESFSFSHDFELWSRAVFAGDFHLLPRYLTYYRRSPGQISSEHSEAQMKHA
                     SRVYSSLLKKMGYRPAAEELSAHLRLTGMDIRPEREQEHYRDILSWCESLYKTNEKSL
                     MVRPYILANEILLRFLKYCSINSFGTFKSLRLLFCLHRRLPFPLFPYFQLFQRVSKKI
                     "
     gene            2138..2905
                     /locus_tag="PLM68_04350"
     CDS             2138..2905
                     /locus_tag="PLM68_04350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT54628.1"
                     /translation="MRFDTAQYRRKEVELQRVDSIMRMLPSHAEQVLEAGCREGYITL
                     KLAGIYPSVTALDLNMPGIVHPRVACVQGDIKALDFPDKSFDLVICTEVLEHIPPQKL
                     EKACAELLRVTRRYLLIGVPFRQDLRVHATRCIHCGSINPTTGHVNSFDLQQLMSLFG
                     NMPFSRTEYPGRGVYKTNRLSYAVYERFGFPYGSYQQEEPCINCGKHLEKPRLSFPAR
                     LACLFANGLEFIQNKTGPSRSPVWIHVLWDITGQDSF"
     gene            complement(2883..5696)
                     /locus_tag="PLM68_04355"
     CDS             complement(2883..5696)
                     /locus_tag="PLM68_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOT54629.1"
                     /translation="MKKFLTTFLVLLLAGIYGYSQMANPQEPLKIDPAVRIGKLDNGL
                     TYYIRHNTKPENRAEFYLFTNVGAIQETKAQAGLAHFLEHMALNGTRNLPGKMLISYL
                     ESIGCSFGRNINAGTGVEQTMYMLNNIPMLREGVLDTCLLIMHDYAAFVTNDPQEIDK
                     ERGVIIEELRTRRTAQWRMFEASLPYLYKGSKYAECNLIGTIEGLQTFPASELHDFYK
                     TWYRPDHQAVIVVGDVDVDQVESKLKALFQDIPAPTTPSPKKVIPVPENDDPIVGIIT
                     DPEAQNTGIEFIIKSEPIPYTMRSLGMIYMVDMFKSFVSRMMNDRFQEIAQQPDAPFL
                     GAGMSFSSITTSADATMFTVQSRDGEGARAFEALMTEVEKMKRYGFTDGELERAKTNF
                     LRRLERAAENAADRMNSEFINTMMSHYAFNEPILDPAYELEVAKGYLPFITLDQLNQL
                     AQQFITEENRILLVSGPEREGLQIPSEQELLDIIRKVKDMEIQAYAEEVSDEPLVNVD
                     AIVPGKIISQQEGAFESVVWTLSNGIKVVIKPTAHKKDEVLFSLQNDGGLSIVPEDLL
                     PSLESNVFTLWSQSNGVGNFNATSLKRKLTGKIANASPFISSYDQGIRGSASPQDIQT
                     LLELVYAHIVTPRFVESEFEAPMAQLKAIVPNMEKTPNFVLQKELMKTVFNNNPRSQL
                     VSSELLEKVSLANLEKAYRLCFANNNGAVVTIVGNVDPEALKPLVELYLGSLPSATKP
                     AVWVKDNNRIRKGMVENHFNVVMETPKTSIVNVYSADMEYNLNNDIYLDAVQSILNMT
                     YTKTLREDEGGTYGASVQKISSSQPEEYGGIIIVFDTDPERQERMSQVVRNDFLNLGQ
                     NGPSDEYVTKTRENFLKNRQENVIRNSFWQQSLISFYTEELDIVTGYEEIVKGITPSS
                     VQEFIKNFLKQNNYIDIKMNPAQ"
     gene            5877..7487
                     /locus_tag="PLM68_04360"
     CDS             5877..7487
                     /locus_tag="PLM68_04360"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT54630.1"
                     /translation="MPCTNLIVTKGASKDGSVIVSYSADSHQLFGELYFWPAAEWAEG
                     TMLKIYEWDTGKYMGEIPQARRTYSVTGNMNEYQLLIGETTFGGRSEFNDPTAMMDYG
                     SLIYLALQRCKTAREAILLIDNLMQTYGYASSGESFSIADPDEVWIMEIMAKAPRMVE
                     GVNVNKGAVWAARHIPDGYISGHANQARITNLDFDDPDNCLYSPDVISHAREMGIFSG
                     KDKDFSFSDVYAPVDFGAARACDARIWAYFNRFADGMDKYLDYAMGHNLTNRMPLYVK
                     PNVKLSFRDVAGMMRDHFEGTPMDMTCDIGAGSNALPYRWRPMSFEVDGKTYVNERAI
                     STQQTGWWFVGQCRSDLPRDLGGLYWFGVDDAGTSALIPFYSVSQRVPRCIEEGNGSM
                     LEYSETSLFWIVNRVAQFAYLRYNQTGAEVRNVVDAWENEMVEKTAHIDQIALTLYKQ
                     DHALAREFLTDFSVNAAQELFDKWVELDKYLMVKYIDGNIKHQNPDGSFTNNGNSVRI
                     PKSPISGGQTQKWKEAVVKDNGTILESR"
     gene            7493..8794
                     /locus_tag="PLM68_04365"
     CDS             7493..8794
                     /locus_tag="PLM68_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT54631.1"
                     /translation="MKRYFLLLLLFLPLLAKGADSSLVFVLNIDSEIHAKSARMLDKA
                     LQEAKEQNAMAFVMRLNTFGGALDAAEKMRTALLEAPFTTVSFIDHQAASAGALISIA
                     CDTIYMAPGSTIGSASVVNQTGEPMPEKYQSYMRSLMRATAEETGRDPRLAEQMVSGD
                     TIVNLTTREAIEAGLSQGQAASVEEAVALLGYENHVIKEYEETWLDKVIGFFLLPLVQ
                     ALMLMGMIGGIYFELQSPGIGFPLGVAVFCAIGYFSPLFLEGLVQYWEIIIIIAGLIL
                     LAVEIFVTPGFGVLGILGLVAIGAGLTLTMIDNRVFDFKGPFPWKDILVPVATVSVTG
                     LLALSFILFSVHMLFPTRVFNRIALRTNLETEDGFVGVPTLHDIVIGQEGVTFTDLRP
                     SGKVTIAGRWVEARAAIGYIPKGTPVKVVRIESGAIFVETL"
     gene            complement(8772..10040)
                     /gene="ispG"
                     /locus_tag="PLM68_04370"
     CDS             complement(8772..10040)
                     /gene="ispG"
                     /locus_tag="PLM68_04370"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOT54632.1"
                     /translation="MSSHVVYAGKLAIGGGHPVSVQTMCNTDTLDVDASIAQCLAVAR
                     EGAQMVRLTTQGPRQVKALAVIREKLRDQGVDIPLVADVHFSAHTAMEAARVADKVRI
                     NPGNFSRDRSRVRELLAELFEICRRHDTALRIGVNHGSLPLHILETYGNSPEGMCQAA
                     MEYLTICQEMDFPRVVVSLKSSNTRIIVQANRLLVQNMKALGMDYPLHLGVTESGNDR
                     QGRLKSAVGIITLLKEGIGDTFRVSLTEAPGAEIRFGKLFLPVAKDRSQPASWDELIV
                     SAAYKWAPGLLDGTIDWKTLPMPFIEAPFASKEELKEFCMDLLQATRCVFSSPEYISC
                     PGCGRTKFNLEETVRQVKKATSHLKGCTIAVMGCIVNGPGEMADAQYGYVGEGNGKVT
                     LYKGKIPVMRGIPQEEAVQRLLELIESQQK"
     gene            complement(10043..10663)
                     /locus_tag="PLM68_04375"
     CDS             complement(10043..10663)
                     /locus_tag="PLM68_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54633.1"
                     /translation="MVLLLLVIPAGAQTVFHKQHLLGIRGGYAFNGVSFNPDRKQKMV
                     PSFYNASILYTYYHDLWGTMPYFGLQTGLTYTQQGYETPDVKRVYDVLRIPLTSQFHI
                     DFWKMRLLINLGCFGSYRMRAFDYTAEKPDGSQVVFDCNHLYLDYGIQGGLGLALVLK
                     PFEFHIEANYLYSLSMIENPALYSNEYYTYGYPNQLLFTLGIFIHL"
     gene            complement(10680..>12330)
                     /locus_tag="PLM68_04380"
     CDS             complement(10680..>12330)
                     /locus_tag="PLM68_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT54634.1"
                     /translation="NKNIMLSPPPQKNPTVRTKGKGIVVELEDELKPNTTYSLYFGNS
                     IQDNNEGNPFPVFALGFSTGEQVDSLMYSGLVLDASTLLPVENTTVVLHINPVDTTIS
                     RTLPVAATRTDAYGYFVLRNLKDTLYSLFAMTDQNNNYRYSQTEGEQIGFLDTLIRPH
                     KRMFTYAPEIQPYYLTDTAGLLRRPVEGNVFLFKEVSARQSLRDYKRIQPRALELKFG
                     APFPTIISAQIPGIDSTQIIRQHNYYRDSLVWWLAAPSVPDTVLLHLTYMATVDSLNK
                     LLPRTDTLRFTPYVDEKAANREKDMAATGRGRGEKQQAPPSVKPGREVMSINVSAVPE
                     RIPDQGIVVRFKSLPIGPDFSLAQLVRITAKEDTVTVPFTVEPDSLDLCLYRIIPATY
                     MESTQYLFSIPSDAFRDVYGLPNDSLQTSFKTLLRDDFGALNLQLENVPNPLIVDLMN
                     EGRNQVLRTSRIASDSTITYPYLKAGKYTVRITEDMNGNGYWDTGNIMERKQAERVRM
                     FRLPSGNPVIDLAEKMELTQIIDIEQLLNQHVSLSVPVKRR"
BASE COUNT         2981 a         2872 c         3160 g         3317 t
ORIGIN      
        1 aatttttttg agcttatcgc tccatgtatt aactttgctg ttgatttaac aaacaaaaca
       61 ataatatgaa taagattaaa gttggaatta acggctttgg ccggattgga cggttggttt
      121 tccgtgctgc ccaggatttt ggaaacattg aaatagttgg tattaacgac ctgatagacg
      181 ttgactatat agcttacatg ctaaggtatg atacagttta cggacaattc cagggttcta
      241 tagaagtcag ggacaacaaa ctggttgtca atggaaaatc catccgcgtt acggcagaga
      301 aagatcctat caaccttaaa tggaacgaga taggcgcaga atatgtagtg gaatccacag
      361 gtcttttcct tacaaaggaa aaagcacagg gacacctggc tgccggagca aaacgtgtta
      421 tcatgtctgc accctccaag gatgacaccc ccatgttcgt cttcggagtc aaccataaat
      481 tatacaaagg cgagcaaatt atttccaatg cttcctgtac gacaaactgc cttgccccct
      541 tagccaaggt gttgaacgat aaattcggga tagtggaagg gcttatgaca actgttcacg
      601 ccaccacagc cacccagaaa acagtagacg gaccttctgc caaggattgg aggggcggac
      661 gcgctgctgc tgccaacatc attccctctt ccacaggcgc cgccaaagcg gtgggcaaag
      721 tgatccccca gctgaacggc aagctaaccg gtatgtcatt ccgcgttcca accgttaacg
      781 tttctgttgt ggatctgacc tgtcgtattg aaaagccggc cagctacgat gaaatcaagg
      841 ccgccatgaa agaagcttcg gaaggtgaaa tgaaaggtgt cctgggttat actgaggatg
      901 ccgtggtttc ttctgatttc ctgcacgatt cacgtacttc catatttgac gcaggagccg
      961 ggatctcact caatcccaat tttgtaaaag tagtttcctg gtacgacaac gaatggggtt
     1021 attcctgcaa gattgtggat atgatccagc acatggctac tgtcaaataa gcagtccttt
     1081 ccatgtaaaa ccggatgttt tctgagggaa tacatccggt ttttttatat ttgcgtatgg
     1141 tttcagtaat tatggctgcg ttcaacgcgc aggcttacat agaagcggca ctggacagcg
     1201 tctttgccca aactttcagg gactttgaga tagtggttgt ggatgatgct tctaccgacg
     1261 gtaccggcca tatactggaa aaccgcaaag acccctgcct tagggttttc aggaacgaga
     1321 cacgcagcgg agccgctttt tcaaggaaca gggctattgc tgaagcttca ggtaagtata
     1381 tggccgtcct ggatgctgat gatttctggg cccctcaaaa gctggaaaaa caggttgaat
     1441 atatggaaaa gcatcctgct gccgccggcc tgggaacgta tgtttacgaa acagaccata
     1501 cggggcaccc ggtcaaggca gtacgtttcc ctttatatcc gggacaaatc agatgctcca
     1561 cattattccg ctgttccctg gtccattcct ccgtgatggt taggcgctct ttcctggtag
     1621 agcagaacct ccggtatgat gaatcctttt ctttttcgca tgattttgaa ttgtggagca
     1681 gggcagtctt tgccggtgat ttccacctgc ttccccgtta ccttacgtat taccgccgca
     1741 gtccggggca gatatcctcg gaacattccg aagcgcaaat gaaacatgca tcccgtgtat
     1801 acagctcatt gttgaagaag atgggctatc ggcctgcagc cgaagagctg tctgctcacc
     1861 tgaggctgac cggaatggat atccggcctg aaagggaaca ggaacattac agggacatac
     1921 tgtcatggtg tgaaagtctt tataaaacga acgaaaagag ccttatggtc aggccttata
     1981 ttttggccaa tgagatcttg ctgcgttttc tgaagtactg ttcaataaat tcctttggta
     2041 cgttcaagtc cttacggctg ctgttttgcc tgcaccggcg tttgccattt ccgttgtttc
     2101 cctatttcca gttattccaa agagtgagca agaaaatatg aggttcgata cagcacaata
     2161 ccgtcgtaaa gaagtggaat tgcaacgcgt ggattccata atgcgtatgc tcccttccca
     2221 tgccgaacag gtgctggagg caggatgccg cgaagggtac atcacactga aactggcagg
     2281 aatttatcct tctgtcaccg ccctggacct gaacatgccc ggtattgtcc acccccgcgt
     2341 agcttgtgtg cagggtgaca tcaaagcact tgatttcccg gacaaatctt ttgatctggt
     2401 catctgtaca gaggtactgg aacatatccc gcctcagaag ctggaaaagg cttgtgcgga
     2461 attgctgcgt gttaccagaa ggtatcttct catcggggtg cctttcaggc aagacctgag
     2521 agttcacgct acccgctgca ttcactgcgg aagcatcaat cctaccacgg gtcatgtcaa
     2581 ctcttttgac ctgcagcagc ttatgtctct cttcggcaac atgcccttct caagaaccga
     2641 gtaccccggc agaggtgtat ataagacaaa ccgtttatcc tacgcggtat atgaacgttt
     2701 tggatttccc tacggttctt accagcagga ggaaccctgc atcaattgcg gaaaacacct
     2761 ggaaaaacca cgtttgtctt ttcctgcacg actggcatgt ctttttgcca atggcctgga
     2821 gttcatacaa aacaagacag ggccctcaag gagccctgtc tggattcatg ttttgtggga
     2881 tattactggg caggattcat tttgatgtca atgtaattat tctgtttcag gaaattcttg
     2941 atgaattcct gtacactgga aggagtaatc cctttgacaa tctcttcgta acctgttaca
     3001 atatccagct cctctgtata gaagctgatg agtgactgct gccagaaact gttcctgata
     3061 acgttttcct ggcggttttt aaggaagttc tccctggttt tggttacata ctcatctgat
     3121 ggaccgttct ggccaagatt aaggaaatcg ttcctgacca cctggctcat tctttcctgt
     3181 ctttccggat cggtatcaaa aacaatgata atgcctccgt actcttcggg ctgactcgag
     3241 cttattttct gaaccgatgc tccataggtc ccgccttcat cttccctgag ggtcttggta
     3301 taggtcatat tgagaatgga ctgaacggca tccaggtata tatcgttgtt caggttgtat
     3361 tccatgtccg ccgaatagac attcacgatg cttgttttgg gcgtttccat cacgacattg
     3421 aaatgatttt ccaccattcc tttgcggatg cggttattgt cttttaccca cacggcaggc
     3481 tttgtagcag atggtaatga acccaggtaa agttccacca ggggtttcaa agcttccgga
     3541 tcaacattac caactatggt gactactgcc ccgttgttat tggcaaagca caatctgtat
     3601 gctttttcca ggttcgccag agacactttt tccagcaatt cactcgaaac cagttggctg
     3661 cgtgggttgt tgttgaaaac ggttttcata agttccttct gaagaacgaa attgggtgtt
     3721 ttctccatat tggggactat ggctttgagc tgggccatgg gggcttcaaa ttcactttcc
     3781 acaaagcgtg gtgtgacaat gtgggcatat accagctcca gcagggtctg aatatcctga
     3841 ggcgaagcgc ttcctctaat gccctggtcg taactactaa tgaagggaga ggcgttggca
     3901 atcttaccgg taagcttgcg tttgagcgat gtggcgttga aattgcctac cccgttgctt
     3961 tgcgaccaga gggtgaaaac gttactttcc agcgaaggca acaggtcttc aggaactatg
     4021 gagagtcctc cgtcgttctg cagggagaat aatacttcgt cctttttgtg cgcagtgggt
     4081 tttataacca ccttgatgcc attggagagg gtccatacca cactttcaaa agcaccttcc
     4141 tgttggctga ttatctttcc tggcactatg gcgtccacgt tgacaagtgg ttcgtcggat
     4201 acttcttctg cataagcttg aatttccata tctttcacct tcctgatgat atccaggagt
     4261 tcctgctcgg aggggatctg caggccttcg cgctccggac cgcttacaag cagaatacgg
     4321 ttttcttccg ttatgaattg ctgtgccagc tggttgagct ggtcaagagt aatgaaagga
     4381 aggtatcctt tcgcaacttc cagttcatat gcggggtcca ggatgggctc attgaaagca
     4441 taatggctca tcatggtgtt gatgaactca ctgttcatcc tgtccgccgc attctctgct
     4501 gcacgttcca ggcggcgcag gaaattggtt ttggcacgtt ccagttcgcc atctgtgaat
     4561 ccataccgtt tcattttctc cacctcggtc ataagggctt caaatgcccg ggcaccttca
     4621 ccatccctgc tttgtacagt gaacatggta gcatctgcag aggtggttat ggaggaaaag
     4681 ctcatgcccg cacccaggaa aggagcatcg ggttgttgtg cgatttcctg aaaacggtcg
     4741 ttcatcattc tggaaacgaa cgatttgaac atatctacca tgtatatcat tcctaaagaa
     4801 cgcatggtgt agggaatagg ttcggatttg ataatgaatt ctatccctgt gttttgtgct
     4861 tcagggtctg taatgattcc gactatggga tcgtcgtttt ccggtaccgg gatcactttc
     4921 ttggggctgg gggtggtagg ggccggtatg tcctggaaaa gcgctttgag cttgctttca
     4981 acctggtcca catcaacatc tcccactaca atgacagcct gatgatcggg gcggtaccag
     5041 gtcttgtaaa agtcatgcag ttccgatgcg gggaatgtct gaagtccttc tatggtacct
     5101 atcaggttgc attcggcata ttttgaacct ttgtacagat agggcagcga ggcttcgaac
     5161 atacgccact gggctgttcg gcgtgtacgc aattcttcta tgatcacacc cctttccttg
     5221 tcaatttctt gggggtcgtt tgtcacaaaa gcggcataat cgtgcatgat cagcaggcag
     5281 gtatccaaga ctccttcccg gagcatggga atattgttga gcatgtacat ggtttgttca
     5341 acccctgtac ccgcattgat gttcctgcca aaactgcaac ctatgctttc caggtaactg
     5401 atgagcatct tgccaggaag attcctggtg ccgttgagtg ccatgtgctc aaggaagtgt
     5461 gccagacccg cctgggcttt ggtttcctgt atggctccca cattcgtgaa aaggtaaaat
     5521 tcggcacggt tttcaggctt tgtgttatgc cttatgtagt aggtaagtcc gttgtcaagt
     5581 ttacctatac gaactgcagg atcgatcttc aagggctcct gtggattggc catttgtgaa
     5641 tagccgtaga ttcctgccaa tagcagaact agaaaagtgg ttaaaaattt cttcatgatt
     5701 tgattggtta ttctatgaat gagtaatgtt caaagaaaac aaagatagta aaaaaacgct
     5761 cggtgtttgt cttttttgta tttttacaaa atagacatta ttaaaaatat atgccaatga
     5821 ttaccagaaa gatcttcctg agccttgtcg ctttttttgt gggtgccggt acagccatgc
     5881 cctgtacgaa tttgattgta accaaaggtg cgtcaaaaga cggatccgtg attgtcagtt
     5941 attccgccga ttcacaccag ttattcggtg agttgtactt ctggcctgca gctgaatggg
     6001 ccgagggaac catgttgaaa atctatgaat gggatacagg taagtacatg ggggaaatcc
     6061 cccaggcacg ccggacctat tctgtgaccg gaaacatgaa tgaataccag ctgttgatag
     6121 gggagaccac ctttgggggc aggagtgagt tcaatgatcc gaccgcgatg atggattacg
     6181 ggtccctgat ctacctggct ttacagcgct gcaaaaccgc acgtgaggcc atcttattga
     6241 ttgataatct aatgcagaca tatggatatg cctccagcgg ggaatcattt tccattgccg
     6301 atcccgatga agtatggatc atggaaataa tggccaaggc tcccaggatg gtggaagggg
     6361 tcaatgtgaa caaaggggcc gtatgggcag ccaggcacat tcccgacgga tacatcagcg
     6421 gtcatgccaa ccaggcccgc atcacaaacc ttgattttga cgatcctgat aattgtctat
     6481 atagtcccga tgtgatttcc catgcccggg agatggggat ttttagcggc aaggacaagg
     6541 attttagctt ttccgacgta tatgcaccgg tggattttgg agccgcccgt gcctgtgatg
     6601 cgcgcatatg ggcttatttc aaccgttttg cagacggcat ggataaatac ctggattatg
     6661 ccatgggtca taatctgacc aaccgtatgc ccttgtatgt aaagccaaat gttaaattgt
     6721 ccttcaggga tgttgccgga atgatgcgtg accattttga aggaacccct atggacatga
     6781 cgtgcgatat cggcgccgga agcaacgcat tgccataccg ctggaggccc atgtcattcg
     6841 aggtggatgg aaagacctac gtcaacgagc gggccatatc aacgcaacag acaggatggt
     6901 ggtttgtagg acaatgcagg agtgatcttc caagggacct tgggggcttg tactggtttg
     6961 gggtggacga tgccggtact tcggccctga taccgtttta cagcgtttct cagcgtgttc
     7021 cccgatgcat agaagagggg aacgggagca tgctggagta ctctgaaaca tcgctcttct
     7081 ggatagtgaa cagggtagcc cagtttgcat accttcgcta taaccagacc ggggctgaag
     7141 tccggaacgt tgtggatgcc tgggaaaatg agatggtgga aaaaactgcc catatcgatc
     7201 agattgcttt gactttgtac aaacaggacc atgctctggc ccgtgaattt ctgactgatt
     7261 tttcggtgaa tgcggcccag gagttgtttg ataaatgggt ggaacttgat aaatatctga
     7321 tggttaagta tattgacgga aacatcaagc accagaaccc ggacgggtca ttcaccaaca
     7381 acgggaacag cgtacgaatt cccaaatccc ccatatccgg aggtcagaca caaaaatgga
     7441 aagaagccgt agtcaaggac aacggtacaa ttttagaatc aagatagatg gaatgaaacg
     7501 ttattttctc ctgcttttgc tctttttgcc cctgctggca aaaggagctg attcaagcct
     7561 ggtatttgtg ttgaacattg acagtgagat ccatgccaag tcggccagaa tgcttgacaa
     7621 ggcgctgcaa gaggcaaaag aacagaatgc catggcattt gtaatgcgtc tcaatacttt
     7681 tggcggtgca ttggatgcgg ctgaaaaaat gaggacagcc ctgctggaag ctcctttcac
     7741 cacagtttcc tttattgacc accaggcagc ctcggcaggt gcactgatct ccattgcgtg
     7801 tgataccatt tatatggccc ccggatccac catcggctct gccagtgtcg tgaatcagac
     7861 tggagaacca atgcccgaga aataccagtc ttacatgcgt tccctgatgc gtgccacggc
     7921 cgaagaaacg gggcgtgatc ccagactggc ggaacaaatg gtcagcggag acacgatagt
     7981 gaatctgacg acaagggaag ccattgaagc aggattgagc cagggacagg ccgccagtgt
     8041 ggaggaagca gtggcattgc ttggttatga gaatcatgtc atcaaggaat acgaagagac
     8101 ctggctggac aaggtgatag gattctttct actgccgctg gtccaggccc tgatgctcat
     8161 gggcatgata ggaggcattt attttgaact gcaaagcccg ggcataggtt tcccgctggg
     8221 ggtggcggtc ttttgtgcca ttggctattt ttccccgctt ttcctggaag gattggtgca
     8281 atactgggag atcattatca ttattgcagg cttgatactt ctggctgttg aaatatttgt
     8341 cactccgggt ttcggggtgt tgggcatcct tggccttgtt gctataggcg ccggtttgac
     8401 tcttaccatg atagacaacc gggtgtttga tttcaaaggg cctttcccct ggaaagatat
     8461 actggtgcct gttgccacgg tgagtgtcac cggtctgctg gccctgtctt ttattctttt
     8521 ttccgtgcat atgttgtttc ccaccagggt gttcaaccgc atagccctga ggacgaacct
     8581 ggaaacagaa gatggatttg ttggggtacc gaccctccat gatattgtca taggccagga
     8641 aggggtgacc tttactgacc ttcggccttc cggaaaagtg accattgcag gcaggtgggt
     8701 ggaagcccgt gcggctatag gatacattcc caaaggtact ccggttaaag tggtacggat
     8761 agaaagcggt gctatttttg ttgagactct ataagttcca gcaaacgctg taccgcttct
     8821 tcctgtggaa tcccccgcat gacggggatt ttccctttat atagggttac cttcccattg
     8881 ccttccccaa catatccgta ctgggcatcg gccatttctc cgggcccgtt cacaatgcaa
     8941 cccatgacgg caatggtgca tcctttaagg tgtgatgtgg ccttcttgac ttggcggact
     9001 gtttcttcca ggttgaattt tgtacgacca caaccgggac aggaaatgta ctcggggctt
     9061 gaaaagacac aacgggtagc ctgcagcagg tccatgcaga attctttcag ttcttctttt
     9121 gaagcaaagg gtgcctctat aaatggcatg ggcaatgtct tccagtcaat ggtcccgtcc
     9181 agcaggccgg gagcccactt gtaagcggca ctgacaatga gctcatccca tgatgcaggc
     9241 tggctgcggt ctttggctac aggcaggaac agcttgccaa agcggatttc cgccccgggg
     9301 gcctcggtga gggaaaccct gaaggtgtct ccaatgcctt ctttaagcag ggtaatgata
     9361 cccacagcgg atttaaggcg tccctgccgg tcgttgccag attctgtcac acccaggtgc
     9421 agcgggtagt ccatacccag ggctttcatg ttttgaacga gcagccggtt ggcctgtaca
     9481 atgatgcggg tattgctcga tttgagagag accacaacac ggggaaaatc catttcctgg
     9541 caaatggtca ggtattccat ggcagcctga cacatgcctt caggagagtt cccgtaagtt
     9601 tccaggatgt gcagggggag agagccgtgg ttcacgccaa ttcgaagggc agtatcatgc
     9661 cggcggcaaa tttcgaagag ctctgccaac aattccctga ccctggagcg gtcacgtgaa
     9721 aaattaccgg gattgatgcg aaccttatcg gccacccggg ccgcttccat agcggtgtga
     9781 gccgagaaat ggacgtcggc gaccagggga atatccactc cctgatccct gagcttttcc
     9841 ctgatgacgg ccagtgcttt aacctgtcgg gggccctgtg tggtaagcct taccatctgg
     9901 gcgccttccc gagcaacggc caggcattgg gctatggatg catccacatc cagtgtgtcc
     9961 gtgttgcaca tggtctggac agaaacgggg tgtccgcctc cgatggccaa ttttccggcg
    10021 tatacgacgt gcgagctcat ggttataaat gtatgaaaat ccccagcgtg aaaagcaatt
    10081 ggttgggata accgtatgtg taatattcgt tgctgtacag agccggattc tctatcatgg
    10141 aaagtgaata gagataattc gcctctatgt ggaattcaaa aggcttcagg acaagtgcca
    10201 ggcccagtcc gccctggata ccgtaatcca aatatagatg attgcagtcg aacaccactt
    10261 gggatccatc gggtttttct gcagtatagt cgaaggctcg catgcggtaa cttccaaaac
    10321 atcccaggtt tatcaacagt cgcattttcc agaaatcgat gtggaactga gaggtcagtg
    10381 gtatacggag cacatcgtaa acccttttca catcgggagt ttcataccct tgctgcgtat
    10441 aggtcaatcc tgtctgaagg ccaaagtagg gcatggtacc ccataagtcg tgataatagg
    10501 tatagagtat ggatgcattg taaaaagaag gtaccatttt ttgcttcctg tcgggattga
    10561 aagacacccc attgaatgca taccctccac gaatccccag caagtgctgt ttatggaaaa
    10621 cggtctgggc tccggccgga atcaccaaca gaagcaatac catgcagagg aatggccgtt
    10681 taccttcttt taaccggaac agatagtgaa acatgttggt tcaaaagttg ttcaatatca
    10741 ataatttgtg tcagctccat tttttcggca agatctatga cgggatttcc ggaaggtaaa
    10801 cggaacatcc tgacgcgttc tgcctgtttc ctttccatga tattgcccgt gtcccagtag
    10861 ccgttcccgt tcatgtcctc tgttatcctg accgtgtatt taccggcttt caggtaagga
    10921 taagtgattg ttgagtcaga agcaatccgg ctggtacgca gtacctggtt ccggccctcg
    10981 ttcatcaggt ctacgatcag agggtttggc acattctcca gctgcaggtt caaagccccg
    11041 aagtcatccc tgagcagtgt cttgaatgaa gtttgcagcg aatcgttggg taagccgtag
    11101 acgtccctga aggcatctga agggatcgaa aaaagatact gggtggattc catataggtg
    11161 gcggggatta tcctgtacag gcaaaggtcc agtgaatcag gctctaccgt gaaaggaaca
    11221 gttaccgtat cttccttggc ggttatacga accagttgtg ccagggaaaa atccggtcct
    11281 atgggcagac ttttgaaccg tacgacaata ccctggtctg gtattctttc cggtactgca
    11341 ctgacattaa tgctcatgac ttcccttccc ggtttgaccg atggaggtgc ctgttgtttt
    11401 tctccacggc ctctgcccgt ggcggccata tctttttccc ggtttgcagc cttttcgtct
    11461 acataagggg taaagcgcag ggtatcggtg cgcgggagca gcttgttcag ggaatcgaca
    11521 gtagccatat atgtcaggtg caagagcacc gtgtcgggga ccgaaggcgc tgccagccac
    11581 cagacaaggc tgtcacggta gtagttgtgc tgacgaatga tctgagtgga atcaattccc
    11641 gggatctgcg cacttataat ggtcgggaag ggcgctccga acttcagttc cagagcacgg
    11701 ggctggattc gtttataatc cctaagggat tggcgtgcgc tcacttcctt gaaaagaaac
    11761 acattccctt ctaccggtct gcgtagcaac ccggccgtat ctgtaagata ataaggttgt
    11821 atttcgggag cataggtgaa cattcttttg tgagggcgga taagcgtgtc tagaaaacct
    11881 atctgttcgc cttctgtctg gctgtaacgg taattgttgt tctggtccgt catggcgaac
    11941 agagaataaa gggtgtcttt caggttcctt aacacaaagt acccataagc atctgtgcgt
    12001 gtggctgcta cgggaagtgt cctgcttatg gtcgtatcaa caggattgat gtgaaggact
    12061 acggttgtgt tttccaccgg gagcagggta gaggcatcca gtacaagccc ggaatacatc
    12121 agggaatcta cctgttcacc ggttgaaaaa cccagggcaa atacaggaaa aggattgcct
    12181 tcgttgttat cctgtatgga gttcccgaaa tatagactgt acgttgtatt tggcttgagt
    12241 tcgtcttcca gttccaccac gattcctttt ccctttgtcc tgaccgtagg atttttttgc
    12301 ggcggagggg aaagcatgat gtttttattg
//
LOCUS       DAOSOF010000053        12086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5242_length_12086_cov_6.359737, whole genome shotgun sequence.
ACCESSION   DAOSOF010000053 DAOSOF010000000
VERSION     DAOSOF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5242_length_12086_cov_6.359737"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLM68_04385"
     CDS             complement(<1..344)
                     /locus_tag="PLM68_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54635.1"
                     /translation="MMKNNLRVILFWSVCWGLAEAVLGFVLHLVENSAGILLYPFGAY
                     CLIMAFRKSGNKAVVPVLVTLITAFLKLSNLAITPPEFHYRVYFPVMAILSEGLVTSG
                     LLFIITMLPVEK"
     gene            complement(331..2613)
                     /locus_tag="PLM68_04390"
     CDS             complement(331..2613)
                     /locus_tag="PLM68_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT54636.1"
                     /translation="MNQSPGKMTDNKSIQYREAAHLAGQTRYLPDLELYEGTLRGYPV
                     SSPYAHARLLELDTSAVMAYPGVATVVTAGDIPGPNTMAPIIDDEPCLLPVGGVCGYA
                     GHVVCLVAADTLETAKKAAALIRWKWEVLPPVLSVEEAMDKRSFLFKPLQLIRGEAGE
                     AMKKAPFREKGSLQAGGQEHYYFETHSAYAVPRHQGGFFVRASTQNPTDNQRFAASLL
                     QLKASDIEVQAGCIGGGFGSKQAQANWCVAWSTLLAWKTKKPVLLILEREQDFRITGK
                     RHPFKASWEVGFDQKGRILAIDLSVAFDCGWCTDVSYAVLHHSIFHIDGAYFIPNLHV
                     MYYPCRTNKTSSTAFRGFGVPQAFSIIESIVTQIARSLGKDAAAVRWQNYYGIKKNNT
                     TPCNMTVDHNILRAAHRQMLEKSNYSALRKQVKEFNKKHISVKRGLAMVPGKFGISFN
                     ESFLNQAGALINIYRDGSVLIHIPGTEMGQGLYDKSRTVVSRELGVSLERIRVSDTST
                     AVIPNTTSTAASTGSDFAGSALKDACDKLKKRLAPIALEMMGLKKGKLDWGNDVIACK
                     EARKTLSFPDLALHAYMELESLSEKGFYKREGIGFELETMEGRPYWYYVMGCAVTLVE
                     LNLLTGEFSIPDVWILHDSGYPIDKGIDLGQIHGAFIQGMGWCTMEKLITDETGQLQT
                     FSLDNYKIPGIYELPDNFHIELFEGNPEPRNIHNSRAIGEPPLIYALSVWLALSDARG
                     KMLSLPATREELIKPLYDEK"
     gene            complement(2585..3994)
                     /locus_tag="PLM68_04395"
     CDS             complement(2585..3994)
                     /locus_tag="PLM68_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOT54637.1"
                     /translation="MDLLRFYLNGKPREIDFCCPDSPPLSCSVLHYLRRYEKMTGTKE
                     VCGSGDCGACTVIVAEKDEVTDTEKWFTADSCLLRLPQLHGKHLLTIEAPDKYMNTTP
                     VRDAFLRERASQCGVCSPGMVLSAYNHVKNNLPLNREEIKRSLSGNLCRCTGYQSIMD
                     AVLSLDSQPGFQAGGEEPLDFPQVEKRMHAFIKGENVYLLPLCLEEFFIARRQYPGAR
                     IASGFTGSCMDHKGSVIDISRVRELLQVSVSEFGISLGAACNYETMKKELGPHFPHII
                     PYLDSFASLQVRHQASLGGNIADGSPVGDTMPLCLALDAVCVACGPEGTRRIKADLFT
                     TGYRKVDLLPDELLMAVVFPFPEKGTHFFAHKQSRRKNMDISSVSFAAAIRLEGRTCT
                     SVRLAYGGMAPVPARAMHTERFLKGKVFSRANCRKASAFIEKDFTPISDIRGSAAYRI
                     DVARNMLLMLYESITGKDD"
     gene            complement(4004..6208)
                     /locus_tag="PLM68_04400"
     CDS             complement(4004..6208)
                     /locus_tag="PLM68_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOT54638.1"
                     /translation="MNTLLALFMTSLIHLIPAPVEAIPGEGYFLFNEQTSIFFNSQAL
                     PLARDAAAYFNETIEPSTGIILKTSKTRPASNVIIFSFDGSLPAEGYTLRVTKDFTEI
                     AAGDGAGYFYGVQTLLQLFPPAVYQPVYQPDQYLPVNQMRLPCVSIKDYPRFSYRGMM
                     LDVSRQFYAVEYVKRFIDWMAKHKLNRFHWHLSDDNGWRVEIKKYPYLTEKGAWRGPG
                     QVLPTTYGHAGQKYGGYYTQQEIRNIVAYAAARYIEIIPEIDLPGHSKAAIASYPDIL
                     CDLTEEVELSIQGTSNNVWCASYEKNYEMLDDIVREMAALFPSRYFHIGGDEVVTSQW
                     ASCARCKAYMHDHQMEDPAGMQAYFAERMQGILNKYGKTLVGWDDIMDGGNLDRKKTV
                     VHAWKGIDKITQAVQEGYSVIAQPASHCYIDMKHTRAERGMTWASISDVDKVYSFDPI
                     LMAGIDPAQAGLVLGIQGGLWSELMDRPARIAEYQVYPRLCALAEVAWSPFAHKDWED
                     FNTRLGQTHFERLYFMGIRFRIPPPKATYANGYIVAENEYPWMVMRYTCDGSEPGPRS
                     PLYTGPIKTDHPEPYRFAAFYRDDIRSLVIEAVLPYDRNQNPVTHVSGNLDLNRRTPL
                     SNLCDNNPATYVQCFGPLQEGQYIQFTFEEPVHTPGIKVLTGLPAVDIDVVDFAHVEY
                     SSDGIDFKRANCAWENGGCRFVPDSPVLSVRLVMDKGNDPLTFYLQDLWIEK"
     gene            complement(6216..6683)
                     /locus_tag="PLM68_04405"
     CDS             complement(6216..6683)
                     /locus_tag="PLM68_04405"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOT54639.1"
                     /translation="MKNFFLFSFFVLCLLPFSAYSQSEKEEQVKAIVESGSFVIRFDR
                     VNPATGRSITLSPFYEMSYDSTRVSCYLPYFGRSYTAPMDPRKLAIELTDKEVEPEIS
                     FNPRRGYTLVFTARTETNELITFYLRISLSGMTTLSINSSSRQSITYLGDLIM"
     gene            complement(6685..7875)
                     /locus_tag="PLM68_04410"
     CDS             complement(6685..7875)
                     /locus_tag="PLM68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT54640.1"
                     /translation="MKHLFYIGILGLCISLSACNNPQENNMLRTGAENTNAYLALLEG
                     KRVGILTNHTALIEGTHLVDSLLSLGVDIRMIFAPEHGFRGDEDAGTRLDHQTDPKTG
                     IPIISVYGSVFIPADSLMRQIDVAVYDIQDVGLRFYTYLSSMYYFMEACARNNVPLLV
                     FDRPNPNGHIVSGPVLDMQFRSFVGIIPIPVVHGMTLGELACMINGEFWLKDSLQTSL
                     TVIPCLNYTHHTLYQLPVKPSPNLPNNRSIYLYPSICLFEATPVSLGRGTDFPFQVYG
                     HPQMAGYDFSFTPQSVPGARNPIQKDVLCYGVDLRTAPPDEVIWSNGFDLQYVIDAYR
                     NLNENAGIGEKFFTSYFEKLVGVDYVRPMILQGKNADEIAMLWYDQLEEFKIKRSQYL
                     IYPQ"
     gene            7945..9888
                     /locus_tag="PLM68_04415"
     CDS             7945..9888
                     /locus_tag="PLM68_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOT54641.1"
                     /translation="MSAILDAKIPEGPLEKKWTIHKESLKTVSPANRKKMHIIVVGTG
                     LAGASAAAALGEQGYRVNVFCMSDSPRRAHSVSAQGGINAAKNYRNDNDSVRRLFLDT
                     LQGGDYRSREANVYRLAQVSNAIIDHCVALGVPFARDYAGYLDNRSFGGAQVSRTFYA
                     RGQTGQQLLLAVYSSLNRQVAAGNVTVHSREQMMDLVTENGQARGIMTRDMVTGRINA
                     YPAHAVVLATGGYGNVYYLSTNAKHSNGSALVQCWKKGAFFANPSFVQIHPTCIPVHG
                     EYQSKLTLMSESLRNEGRIWVPTRKEDVLALREGKIKPSDIPEERRDYYLERRYPSYG
                     NLVPRDVASRAARERCDAGFGVGKTGRAVFLDLSGAIAKDGKACIESKYGNLFQIYEK
                     ITGDDPWKEPMQIYPAIHYTMGGLWVDYNLMTTVKGLYAIGEANFSDHGANRLGASAL
                     MQGLADGLFILPYTISDYLAGKIGEPCPDTATPPFVQVRGDVQKRIDRIMALKGNVPV
                     DVFHRELGNMMWEHAGIAKSAEGLSSLISRLGALEERFRNEAGITGSQATVNQELEKA
                     IHLEDYFTLAGLMVQDALHREESCGSHFRIESQTKDGETLRDDDRFQYVAAWEYKGQA
                     EAPLLYREPLVYEETRMTERKYI"
     gene            9890..10627
                     /locus_tag="PLM68_04420"
     CDS             9890..10627
                     /locus_tag="PLM68_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOT54642.1"
                     /translation="MFFILKVWRQAGPRSRGHFQLFDMDGISGDTSFLEMLDILNNRL
                     VSQGKETIAFDHDCREGICGACGLYINGRPHGPDDGITTCQLYMRRFRDGSVITVEPW
                     RSAAFPVIKDLMVERKAFDKILQAGGFISANTGSAPEAHTTLIARKVAEESMDASACI
                     GCGACVATCKNRSAMLFVASRVSSLALLPQGKPEAARRARAMVAKMDELGFGSCTNIG
                     SCQMECPKNITITHIARLNREFLYRPG"
     gene            complement(10864..12021)
                     /gene="hflX"
                     /locus_tag="PLM68_04425"
     CDS             complement(10864..12021)
                     /gene="hflX"
                     /locus_tag="PLM68_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT54643.1"
                     /translation="MDILKEKDKAVFVGVLRSGTDMETFKDYLDELEFLADTAGLVSG
                     GRFYQRLEKPHPVTYLGGGKLEELAAYIKAENVSIAVFDDELSSSQLRNLDKVLGCRV
                     LDRTNLILDIFAQRAKTAYAKTQVELAQYQYLLPRLTRMWTHLERQRGGIGLRGPGES
                     QLETDRRIILDKIALLKEKLTKIDRQMMSQRGNRGALTRMALVGYTNVGKSTIMNLLA
                     KTDVFAENKLFATLDTTVRKVVVDNIPFLLSDTVGFIRKLPTQLVESFKSTLDEVRES
                     DVLIHIVDVSHPHFEEMIHVVQNTLRDIGCGDKPVYLVFNKIDAFLERQTDEGFTSLE
                     NLKKTWISKLHDPCIFISAKEKINIDKFRKDLYNMAAEVQAGRYPFGTFLW"
BASE COUNT         3049 a         3005 c         3121 g         2911 t
ORIGIN      
        1 aatttttcca caggcagcat tgtaattatg aacaagagcc cggaagtgac caaaccttct
       61 gaaagtatgg ccattacagg aaaatatacc ctgtaatgga attcaggcgg ggtaatggcc
      121 aggttgctca atttgagaaa agccgttatc agtgtgacaa gtacgggaac aaccgccttg
      181 ttgccgcttt tcctgaaagc catgataaga cagtatgccc cgaagggata aagcaggatc
      241 cctgctgaat tctctaccaa atgcaataca aaacccagaa cggcttctgc cagtccccag
      301 cagacagacc agaataaaat cacgcgcagg ttatttttca tcatacaaag gttttatcaa
      361 ttcttccctg gtagcgggca gggaaagcat ttttcccctc gcatcggata atgctagcca
      421 tacagaaagc gcataaatca gcggaggctc tcctatagcc ctggaattat gtatattacg
      481 tggttcagga ttaccctcga ataattcgat atgaaaattg tccggaagct cataaatccc
      541 gggaatctta taattatcca gggaaaaagt ctgcaattgc cccgtttcgt ctgtaataag
      601 tttttccatg gtgcaccacc ccattccctg tatgaacgcg ccgtgtatct gtcccaggtc
      661 tattcctttg tctatgggat atccgctgtc gtgcaggatc cagacatccg gaatgctgaa
      721 ctcaccggtc agcaggttca gctccaccag cgtaacggca caacccatca cataatacca
      781 gtaagggcga ccctccatgg tttccagttc aaatcctatt ccttcccttt tatagaaacc
      841 cttttcactc agagattcca gctccatata ggcgtgtaag gccaggtcag gaaaagacag
      901 ggttttgcgg gcttctttgc aggcaatgac atcatttccc cagtccagct tacccttttt
      961 caatcccatc atttccaggg cgatcggtgc aagacgcttt ttcagtttgt cacatgcgtc
     1021 tttcagcgct gaaccggcaa aatcactccc cgttgaagcg gctgtagagg tagtgtttgg
     1081 aataacagcc gtagaagtat cgctgaccct tatccgctct aaggaaacac caagttcccg
     1141 ggataccacg gtcctggact tgtcgtacaa tccctgcccc atttctgtac cgggaatatg
     1201 gatcagcacc gaaccgtccc ggtaaatatt gataagtgct cctgcctggt tcaggaaact
     1261 ttcattgaaa gaaataccga acttcccggg aaccatggca agaccgcgtt tgacagaaat
     1321 gtgtttctta ttgaattcct tcacctgttt gcgcaaggcg ctgtagttgc ttttttcaag
     1381 catctgccgg tgggcggcac gcagtatgtt gtgatccacc gtcatattgc acggtgtggt
     1441 gttgtttttc tttatgccgt aataattctg ccaccgaacc gccgcggcat cctttcccag
     1501 ggaacgggca atctgagtca ctatggattc aatgatggaa aaagcctggg gaacaccaaa
     1561 acccctgaag gcggtgctgc tggttttatt ggtcctgcat ggatagtaca tgacatgcag
     1621 gttgggaatg aagtaggccc cgtcaatgtg gaaaatgctg tgatgaagga cggcatagga
     1681 aacatcggta caccatccgc agtcaaaagc cacagacagg tcaatggcca ggatcctgcc
     1741 cttctgatca aagccaacct cccagcttgc tttgaaagga tggcgtttgc cggtgatacg
     1801 gaaatcctgc tctctttcca ggatcagcaa aacgggcttt tttgtcttcc atgccagcaa
     1861 agtggaccag gccacacacc agttcgcctg tgcctgcttt gacccgaaac cacccccgat
     1921 gcaccccgcc tgaacttcaa tgtcggaggc tttgagctgt aataatgagg ctgcaaaccg
     1981 ctggttatcc gtaggatttt gggtggaggc gcgtacaaag aatcctccct ggtgccgtgg
     2041 aactgcataa gccgaatgtg tttcaaagta gtaatgttcc tgtcccccgg cctgcaggga
     2101 ccctttttcc ctgaaagggg cctttttcat ggcctccccg gcttctcccc tgatcagctg
     2161 cagcggcttg aacaagaagg atctcttatc catggcttcc tcaaccgaaa ggacgggagg
     2221 caggacctcc cacttccacc gtatgagggc agccgctttc ttggcagtct ccagcgtatc
     2281 tgcggccacc aggcagacga cgtgtcctgc ataaccgcaa actcctccta cgggcagcag
     2341 gcagggctcg tcatctatga taggggccat cgtgttggga ccgggaatat cccctgccgt
     2401 aaccactgtg gctacaccgg gataagccat taccgccgat gtgtccagct ccagcaaacg
     2461 cgcatgggca taaggtgatg agaccggata ccccctgagc gtgccttcat aaagttccag
     2521 gtcgggaaga tagcgggtct gacccgcaag atgtgctgct tccctgtatt gtatggattt
     2581 attgtcagtc atctttcccg gtgattgatt catatagcat caacagcata ttcctggcca
     2641 catctatccg gtatgcagcg ctgccacgga tatcggaaat gggagtaaag tccttttcaa
     2701 tgaaggccga ggctttccgg caattggcac gtgaaaacac ctttcctttg agaaaacgct
     2761 ccgtatgcat ggcacgtgcc ggaacaggcg ccatgcctcc gtaagccagt cgcaccgagg
     2821 tgcaggtcct tccctccagg cggatagcgg cggcaaagga aacggaactg atatccatgt
     2881 ttttgcgcct tgactgctta tgggcaaaga aatgcgttcc tttttcggga aaaggaaata
     2941 ccacggccat gagcaactcg tcgggcagca ggtctacctt acggtaacct gttgtgaaaa
     3001 gatccgcttt tatacggcgt gtcccttcag ggccgcaggc aacacaaaca gcgtcaaggg
     3061 ccaggcaaag cggcatggta tcccccacag gggaaccatc ggcaatgttc cctcccagcg
     3121 aggcctgatg acggacctgc agggatgcaa aagaatccag gtatggtata atatgaggaa
     3181 aatgcgggcc cagttccttt ttcatggttt cataattgca tgctgccccc agggatattc
     3241 cgaattccga aacgcttacc tgcaataatt cccgcaccct ggatatgtca atgacagaac
     3301 ctttatggtc catgcacgag cctgtgaaac ccgaggcaat ccgtgctccg gggtattgcc
     3361 tgcgggcaat gaaaaattcc tccaggcaca ggggaagcag gtaaacgttc tctcccttta
     3421 tgaaggcatg catccgtttt tccacctgcg ggaaatcaag aggctcttcc ccgccggcct
     3481 ggaagcccgg ttggctgtcc agggaaagca ccgcatccat gatggactgg tagccggtac
     3541 aacggcacag gttccccgat aatgaacgtt tgatctcctc cctgttcagc ggaaggttgt
     3601 ttttcacatg gttgtaggcc gataagacca ttcccggcga acatacaccg cactggctgg
     3661 ctctttccct taaaaaagca tcccttaccg gcgtggtatt catatatttg tcaggagctt
     3721 ctatcgtaag cagatgcttt ccgtgaagct gggggagccg caggaggcag gagtcggccg
     3781 taaaccattt ttccgtatcc gtcacctcgt ctttttcagc cacgatcacc gtacaggctc
     3841 cgcagtcccc gctgccgcag acctccttcg tccccgtcat tttttcatac cggcgcagat
     3901 aatgcaggac ggaacaggat aagggcggac tgtccgggca gcaaaaatct atttcccttg
     3961 gttttccatt cagataaaag cgaagcaggt ccatgatctg tttttatttt tctatccaga
     4021 ggtcctgtaa atagaatgtc agggggtcat tgcccttgtc cattacaagg cggaccgata
     4081 atacaggaga atccgggaca aagcggcaac cgccgttttc ccaggcacag tttgcgcgtt
     4141 tgaagtcaat tccgtcggaa gaatactcca catgggcaaa atccacaacg tcaatatcta
     4201 ctgccggcaa acccgtcagg acctttatcc cgggcgtatg gaccggttct tcaaaggtga
     4261 actggatgta ttgtccttcc tgcaacgggc cgaagcattg tacataagtg gcggggttgt
     4321 tgtcgcacag gttggaaagg ggtgttctcc ggttgagatc caggttcccg ctaacatggg
     4381 tcacgggatt ctgattgcgg tcgtaaggca ggacggcctc aattaccagg ctacggatat
     4441 cgtccctgta aaaggcagca aaacggtaag gttcaggatg atctgttttt atgggcccgg
     4501 tatacagggg cgagcgcggt ccaggttcgg atccgtcgca ggtatagcgc atgaccatcc
     4561 atggatattc attttctgcc acaatgtacc cgtttgcata agtggcctta ggtggcggaa
     4621 tacggaaacg gatgcccatg aagtacaacc gttcaaagtg tgtctgccca agccgcgtat
     4681 tgaaatcttc ccagtcttta tgggcaaagg ggctccatgc cacctcggca agggcacaga
     4741 gccgggggta cacctggtat tccgcgatgc gcgcaggcct gtccatcagt tcggaccaca
     4801 aacctccctg tatgcccagg acaagtcctg cttgcgcagg atcaatgcct gccatcagga
     4861 tgggatcaaa tgaatagacc ttatccacat cggaaattga tgcccaggtc atcccgcgtt
     4921 cagcccgggt gtgcttcatg tctatataac aatgcgatgc cggctgggca atgacactat
     4981 atccttcctg aacggcctgt gttatcttgt caatcccttt ccaggcgtgt acaacggttt
     5041 tttttcggtc caggttcccg ccatccatga tatcgtccca ccctaccagt gtttttccat
     5101 atttgttcag gattccctgc atgcgctctg cgaagtaggc ctgcatgccg gcaggatctt
     5161 ccatttggtg gtcgtgcatg taagctttgc agcgtgcaca gctggcccac tggctggtta
     5221 ccacttcgtc ccccccgatg tgaaaatacc gggaaggaaa aagcgccgcc atttcacgca
     5281 caatatcatc cagcatctca taattctttt cataagaagc acaccagaca ttgttgctgg
     5341 tcccctggat gctcagttcc acctcttctg tcaggtcgca gaggatgtca ggatagctgg
     5401 caatggcagc cttggaatga ccgggaaggt caatctccgg gatgatctca atataacggg
     5461 cggcagcata ggccacaata ttacgtattt cctgctgggt gtagtatccg ccgtacttct
     5521 gtcctgcatg gccgtaagtc gtgggcagga cctgtcccgg gccacgccag gctccctttt
     5581 ctgtcaggta agggtacttt ttgatttcca cgcgccagcc gttgtcatcg gaaagatgcc
     5641 agtggaaacg gttgagcttg tgttttgcca tccagtcaat gaaacgtttg acgtactcga
     5701 cagcatagaa ctggcggctc acatccagca tcatcccgcg gtatgagaac ctgggatagt
     5761 cttttatact aacgcaaggc aaccgcatct gattcaccgg cagatattgg tcgggctggt
     5821 aaaccggttg gtatacggcg ggcggaaata gttgcagcag cgtctggaca ccatagaaat
     5881 atccggcacc atcaccggct gcgatctctg taaaatcctt agtcacccgc aacgtgtaac
     5941 cctctgcagg cagggatcca tcgaaagaaa aaatgatgac attggaagcc ggacgggttt
     6001 tgcttgtttt taaaataatc cctgtcgaag gctctatggt ttcattgaaa tatgcggccg
     6061 catcccgggc aagaggcagg gcctggctgt tgaaaaagat ggaagtctgt tcgttgaaga
     6121 ggaaataacc ttccccgggt attgcttcaa cgggagcagg gattagatgg atcagggaag
     6181 tcataaaaag ggctagtaag gtattcataa aaaagttaca ttatcagatc tcccaggtaa
     6241 gtgatgcttt gccgggaact gctgttaata gaaagagttg tcattccgct caggctgatc
     6301 ctgagataga acgtgataag ttcgttggtt tctgtccttg cagtgaagac aagggtatat
     6361 cccctccggg gattgaaaga gatttccggt tccacctcct tgtctgtaag ttctatggcc
     6421 agtttgcgcg gatccatggg agcagtgtat gaacggccga aatacggcag gtaacaagat
     6481 acgcgggtgc tgtcataact catttcataa aaaggggaaa gcgtaatgga acgcccggtg
     6541 gccggattca cccggtcaaa ccggatcaca aaactgccgc tttcaacaat ggctttaacc
     6601 tgttcttctt tttcagactg gctgtatgcc gaaaagggaa gcaggcaaag cacgaaaaaa
     6661 gaaaaaagga agaaattttt catgttattg cggataaatc aggtattggc tgcgtttgat
     6721 cttgaattcc tccagctgat cataccaaag catggcgatt tcatcggcat tcttaccctg
     6781 gaggatcatg gggcggacgt agtccacgcc aacaagtttt tcaaaatacg aagtgaagaa
     6841 tttttccccg atccctgcat tttcattcag gttgcggtaa gcgtctataa cgtattgaag
     6901 gtcaaacccg ttagaccaga tcacttcgtc tggcggagcg gtgcgaaggt ccacgccgta
     6961 gcagagaaca tctttttgaa taggattgcg ggctcccggt acagattgcg gtgtgaagga
     7021 aaagtcataa cctgccattt gaggatgtcc gtaaacctgg aagggaaaat cggtgccgcg
     7081 tccaaggctt acaggggtag cctcgaaaag gcatatggag ggatacaggt atatggaacg
     7141 gttattgggt aggtttggcg atggcttcac gggcaattga tacagggtat ggtgcgtgta
     7201 gttcaggcag ggaatgacgg tcagggaagt ttgcaggctg tctttaagcc agaactcacc
     7261 gtttatcata caggccagct ctccaagcgt catgccgtgc acgacgggta tcgggatgat
     7321 tcccacgaaa gaacggaatt gcatgtccag tacaggcccg gagacaatgt gaccattcgg
     7381 gttgggcctg tcaaagacca gcaggggcac gttgttcctg gcacaggctt ccataaagta
     7441 atacatggaa gacaggtagg tgtaaaaccg aagccctacg tcctgaatgt cgtaaacagc
     7501 aacatcaatt tgccgcatca ggctgtcggc cgggatgaaa acggaaccgt acactgatat
     7561 tatgggaatc cctgttttgg ggtctgtctg gtgatcaaga cgggtgcccg cgtcttcatc
     7621 gcctctgaat ccgtgttcag gagcgaaaat catgcggata tccaccccca gggaaaggag
     7681 gctgtctacc aggtgtgttc cctcgatcag ggcagtgtgg ttggtaagaa ttcctaccct
     7741 ttttccctca agcagcgcaa ggtaagcatt tgtgttttcc gctccggtgc gaagcatgtt
     7801 gttttcctgc gggttgttgc aggcagacag ggatatgcag agtcccagaa ttccgatata
     7861 aaataagtgt ttcatgcaac taagttatga aaaaattgta tctcgtcatt gttttgtatt
     7921 tttgtctgac aaacacacag aatcatgtct gccatcctgg atgcaaaaat tccggaaggt
     7981 ccgctggaaa agaaatggac gattcacaaa gagtccctga aaacggtgtc tcctgcaaac
     8041 aggaaaaaga tgcacattat tgtcgtgggg acagggcttg caggggcctc tgcagccgca
     8101 gcgctgggtg aacaggggta ccgggtgaat gttttttgca tgagcgattc accacgcagg
     8161 gcacactctg tctcggccca gggaggtatc aatgcggcaa aaaattacag gaacgacaat
     8221 gattctgtca ggcgtctgtt cctggacacc ttgcagggcg gggactaccg ttccagggaa
     8281 gccaatgtgt acaggctggc ccaggtaagc aacgccatta tagaccattg tgtcgccctg
     8341 ggcgttccct ttgcaaggga ttacgcggga tacctggata accgctcctt tgggggagca
     8401 caggtaagca ggacatttta tgcaaggggc cagacggggc agcagcttct gctggcggtc
     8461 tattcttcgc tcaacaggca ggtggccgcc gggaacgtaa cggtgcacag ccgggaacag
     8521 atgatggacc tggtaacgga aaacggacag gcccgtggga tcatgacccg ggacatggta
     8581 acgggcagga tcaacgccta cccggcacat gccgtggttc tggctacagg tggttacggg
     8641 aatgtctatt atctttccac caatgcaaag cattccaatg gatcggcttt ggttcagtgc
     8701 tggaaaaaag gggccttttt cgccaatccc agctttgtcc agatacatcc cacctgcata
     8761 cccgtacatg gagagtacca gtccaagctt accctcatga gcgaatcgct ccgtaatgaa
     8821 gggagaatat gggtgcccac ccggaaagaa gacgtactgg cgttgcgtga gggaaaaatc
     8881 aagccatcgg acataccgga agagcgcagg gattattacc tggaaagaag gtatccctca
     8941 tacgggaacc tggtgcccag ggatgtggct tcccgcgccg ccagggaacg ctgtgatgcc
     9001 ggattcggcg tgggaaaaac aggacgagcc gttttccttg atctgtcggg ggcaattgca
     9061 aaagacggga aagcatgcat agagagcaaa tacgggaatt tattccagat atacgagaaa
     9121 ataacgggag atgatccctg gaaagagccc atgcagattt accccgctat tcattacacc
     9181 atgggcggtt tatgggtgga ttacaacctg atgaccaccg tgaaaggctt atatgcaata
     9241 ggagaggcca atttcagcga tcacggggca aacaggctgg gagcctctgc cctgatgcag
     9301 ggactggcag acggtttatt catattaccg tatacgattt ccgattacct ggcaggaaaa
     9361 ataggagaac cctgcccgga cacggctacg cctccctttg ttcaggtccg cggggatgtc
     9421 cagaaaagaa tagacaggat catggcgtta aagggcaatg taccggtgga tgtttttcac
     9481 agggaattgg gaaacatgat gtgggagcat gcagggattg caaaatctgc agaaggtctt
     9541 tcctcactca tttcccggct tggagcgctt gaagaacgct tcaggaatga agcagggatt
     9601 accggaagcc aggccacggt caaccaggaa ctggaaaaag cgatccacct ggaggattat
     9661 ttcacgctgg ccggactgat ggtgcaggac gcactgcacc gcgaagaatc atgcggaagc
     9721 catttccgta ttgaaagcca gacaaaggat ggagaaacat tgcgcgatga tgaccgtttc
     9781 caatatgtgg cagcatggga atacaaagga caggcagaag cgcctcttct gtacagggaa
     9841 ccccttgttt atgaggaaac aagaatgaca gaacgtaagt atatataaga tgtttttcat
     9901 attgaaagta tggcgccagg caggacccag gtccagggga catttccagc ttttcgatat
     9961 ggatgggatt tcaggggata cttctttcct ggaaatgctg gacatactca ataaccgcct
    10021 ggtctcacag gggaaggaga caattgcctt cgaccacgac tgcagggaag ggatctgcgg
    10081 tgcctgcggg ttgtatatca acggaaggcc gcacggcccg gacgatggta taaccacctg
    10141 ccagttgtat atgcgtcgtt ttcgcgacgg cagtgtcata acggttgaac cctggcgtag
    10201 cgcagctttt cctgtgataa aggacctaat ggtggaaagg aaggcttttg acaagatcct
    10261 gcaggcagga ggctttatca gcgcaaacac cggaagcgcc ccggaggccc atacaaccct
    10321 catagccagg aaagtggccg aggaaagcat ggacgcttcg gcatgtatag gctgcggagc
    10381 atgtgtcgcc acctgcaaga accgttcggc catgttgttt gtggcttcca gggtgagctc
    10441 actagctctt ttaccacagg gaaaacccga ggcggccagg agggctcgtg ccatggtggc
    10501 aaaaatggat gagctggggt tcggttcctg caccaatatc ggttcctgcc agatggaatg
    10561 ccccaaaaac atcacaataa cccatattgc ccgccttaac cgcgaattcc tctaccgtcc
    10621 cgggtgagta cggcgtatgc ctcattcatt ccactgaatg tcaattatat aaccaaacag
    10681 ctctcccttg gagctttttg gaatcctgag acttttggtt gatatgctct ctgcttctgc
    10741 cgctgatacc acaagaaaag ggaagcctag gcctaaataa aaagcttccc gtttttgtgg
    10801 tatcagcggc gccttctggc aaccacacaa ataggtgctt cggcacatca aaagtatgca
    10861 gcgctaccac aggaaagttc cgaaggggta cctgccggcc tggacctctg cagccatatt
    10921 atataaatcc tttcgaaact tgtcgatatt tattttttct ttggcagaaa tgaagataca
    10981 gggatcgtgc agtttggaaa tccacgtttt tttaaggttt tcgagacttg tgaagccttc
    11041 gtcggtctga cgttccaaaa atgcgtcgat cttgttaaaa acgagataaa caggcttatc
    11101 tccgcatccg atatcacgga gcgtattttg caccacatgg atcatttcct caaaatgagg
    11161 atgtgataca tccactatat ggatcagaac atcgctttca cgcacttcat ccagggtgct
    11221 tttaaatgat tccacaagct gggtgggcaa ttttctgata aagcctaccg tatcactcag
    11281 aagaaaggga atattatcga cgactacctt gcggacggtg gtgtccaatg tagcaaaaag
    11341 cttattttcg gcaaaaacat cggtttttgc caaaagattc atgatggtgc ttttaccgac
    11401 attcgtataa cctacaagag ccatccttgt taatgcgccc ctgttgcccc gctgagacat
    11461 catctgccgg tctattttgg ttagtttttc cttgagcaag gcaattttat ccaggatgat
    11521 acgccggtcg gtttctagct gcgactcgcc gggaccacgc aaacctattc cgccgcgctg
    11581 cctctccagg tgtgtccaca tgcgtgtcag acgaggcaaa agatattgat attgagcaag
    11641 ttcaacttgt gttttggcat aggctgtctt ggcacgctgt gcaaaaatgt caagtatgag
    11701 atttgtcctg tccaggacac ggcagccaag aaccttgtcc aggttacgta attgtgaaga
    11761 agacaattca tcatcaaaaa cagcgatgga aacattttca gccttgatgt atgcagccag
    11821 ttcctcaagt tttccaccac ccagataagt gacgggatgc ggcttttcca gacgctggta
    11881 aaagcgtccg ccactgacaa ggcctgcggt gtcggcaaga aattcaagct catccaggta
    11941 atccttaaaa gtctccatat cggttcccga ccgtaacaca cccacaaaaa cagctttatc
    12001 tttttctttc aggatatcca tagattcaaa tccttaatcc cgcaacattt ttcgtaaaag
    12061 attgtcaaac ggcctgataa acaaag
//
LOCUS       DAOSOF010000054        11873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5344_length_11873_cov_5.887206, whole genome shotgun sequence.
ACCESSION   DAOSOF010000054 DAOSOF010000000
VERSION     DAOSOF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5344_length_11873_cov_5.887206"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLM68_04430"
     CDS             <1..654
                     /locus_tag="PLM68_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT54644.1"
                     /translation="DPSRYTILALLPSYLERPGSSLVYMMQVLMEKSGTGAGGFYLSD
                     QEALYGRLLDLSTQGKPVWLIGAAFALLDFVDKFTLRHPGLIVVETGGMKGRGTELTR
                     EALHERLRRGFPYSPIHSEYGMAELFSQAYACTDDLFFRCPPSMQVMIRNLQDPFSPE
                     PDGSAGGINIIDTANSHSCSFIETEDLGFRIPGGQFQVLGRIPDAERRGCNMLIDIS"
     gene            complement(638..3691)
                     /locus_tag="PLM68_04435"
     CDS             complement(638..3691)
                     /locus_tag="PLM68_04435"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOT54645.1"
                     /translation="METKTFKKADLLIGWVVFAISAAVYLATIEPTASFWDCGEFIAS
                     SYKLEIGHPPGNPTYQLAGRLFSMLGSDQKAAMLINAMSALASAFCVLFLFWTISHLG
                     RRLLETQKISLNRGNAIAIWGAAAAGSLIYSFSDTAWFSAVEAEVYGMSSLFTAAVFW
                     AMLKWEENADKPHANRWIVLIAYLMGLSIGVHLLNLLTIPALVYIYFYKKYPFTWKGA
                     VVTLLLSVVIIAVVLYGIIPWVPKISGWFDLLFVNVFKLPFNTGMLFFVVVLIAALFW
                     GVFSTYKKGKVLWNTILLCLAVIIIGYSSFAMVVIRASANTPTNENQPDNPFSLGKYL
                     AREQYGAAPLFYDGIYNSDYETWKDTRQYVKMNGRYARVKGPEMPVYKESEKMLFPRM
                     WSKRESHAAFYDSYNSGKGTLNSNGKHMPTFGDNLRFFFDYQVNYMYWRYFMWNFAGR
                     QNDIQGTIPGDPIRGNWESGIGFLDKARLGDQSGAPDMLKNNKGKNHYYMLPLLLGIV
                     GLIYQSLKDRRNAWITFLLFFMTGLAILVYLNQPPYQPRERDYAYAGSFYAFAIWAGL
                     GVMALFNLFQKKLSQTIAGSLAAALALFVPFQMLAQNWDDHDRSGRYTAPAFAFNFLN
                     TCKPDCNAIIVTHGDNDTFPLWYAQEVEGIRTDVRVINTSLLGTDWYIDQMQLRMYES
                     APVTFSIPRESYLYGTNEIIPVYERVKERVPAIQAVGVMGNPRAKVQLMDGTMLNYLP
                     ARKLRIPVNKENALACGIVPEKDAHLIPDYMDLDIPENKNTLMKVEMMFLDLLANYQW
                     DRPIYIVSKGGDLNIGIQDYLRYDGMAYKLMPFKVDSLSTQVPYMDTDKLYHLLMDIY
                     QWGRMDEPDVLLDYHVTYLYLVQQAIRQMYSQVAKAMLLEGKEDKAEALLDKGIDIMK
                     QYPLNWVVQPSMNEVGVMETIELYYFLGKPEKASALAVPFLDETFKAIEYFMGSFRGG
                     LLSPSDAESAISMYVHVKDILDVNEDKELAGTYTQQLEELFKKYQ"
     gene            3746..4381
                     /locus_tag="PLM68_04440"
     CDS             3746..4381
                     /locus_tag="PLM68_04440"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOT54646.1"
                     /translation="MDYIVLGLIVWYCFILFFIRTPLTLLLTGCLRYNISIENEEQVN
                     LRFSRVFMFLFVITLASFFIVSHDTLLSPLPHFLLGMSKPMVFLCIMVFFIVLILVRT
                     FFTKIIGWTIDSSAFMNFLNRTAYDFCILTGFVFIPFLILFFALGNPSGNGVIIAAVA
                     TAAAGYLVYAYRCIRIFLSAGFSLFFWILYLCIFEMIPLGVLIHFVAGIQT"
     gene            4378..5118
                     /locus_tag="PLM68_04445"
     CDS             4378..5118
                     /locus_tag="PLM68_04445"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOT54647.1"
                     /translation="MKIKNILITQPAPNGGSPYLEIKEKFNLNIDFIPFIRVEGLSAR
                     DFRSQRVNLQDHSAVVFTSRSAIDSFFHLCEEMRLTIPESLKYFCMTESVAFYLQKYI
                     IYRKRKIFYGKGNIPSLIETIGSKHKNDTFLLTLADCYKPELPQAFDKAGLKYTKAIL
                     THTIIEDLSPVELKKYQMMVFYSPGDVRSLLENFPDFKQDSVLFGTFGANTATALKEA
                     NLQACFEAPTPEAPSVAQALMLYLEKHK"
     gene            5121..5510
                     /locus_tag="PLM68_04450"
     CDS             5121..5510
                     /locus_tag="PLM68_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54648.1"
                     /translation="MKRTTCLCMGFLLAMITLSCQKNFNHYRELAYGKWELVSVNGES
                     PVAAEQLYFKTSTSVTLTREGVSYEGQYQFYCNVLTTYFEQYQNLFQEWRVQTMKDSL
                     MRAVVEFREMNSDTLDAGSVLEYRKIP"
     gene            5510..5974
                     /gene="rnpA"
                     /locus_tag="PLM68_04455"
     CDS             5510..5974
                     /gene="rnpA"
                     /locus_tag="PLM68_04455"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT54649.1"
                     /translation="MKTGSTYAFPKKMRLSSRKEIESLFTDACSFTTGPFRLFYRTCD
                     VHEPDQPKTDLPETEKGGCSLVFSVPKKNLKSAVKRNLIKRRLRESFRLHYPRLLKPV
                     LEATGTRLVFLCIYLPHEVRPFSYVETKMQVLLGHFAQLSEKGSDFSPDPAG"
     gene            5934..6155
                     /gene="yidD"
                     /locus_tag="PLM68_04460"
     CDS             5934..6155
                     /gene="yidD"
                     /locus_tag="PLM68_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT54650.1"
                     /translation="MKKVLTFPLILLVKCYQLCISPFTPPSCRFIPTCSQYALEALRR
                     HGPLKGLYLAIRRFLRCNPWGGSGYDPVP"
     gene            complement(6335..8164)
                     /gene="uvrC"
                     /locus_tag="PLM68_04465"
     CDS             complement(6335..8164)
                     /gene="uvrC"
                     /locus_tag="PLM68_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT54651.1"
                     /translation="MYFYLEMIQEEDQLALLPHQCGVYRFLNREGKVIYVGKAKDLKK
                     RVSQYFAPGRNLSAKTKRMVSQVQSLEYTVVDNESDALLLENNLIKTIQPRYNILLKD
                     DKTFPWICVKNEPFPRVFQTRRIVRDGSYYFGPYTSGFYCKQLLQLLHNLYPLRTCSL
                     PLAPQAVTAGRYRKCLQAHIGRCKAPCTGEETSEQYQEYISSVSSILKGDVTTVRKLL
                     ESQMKAASQAFNFELAQRYKEQWEMLTRYQAKSVIVNSRISNLDVFSLTTDAGSSMAF
                     GNFMRVADGAVIQSLNVKYKLHIEEEPAAVLSLFMAEMKEQLGQLSAEILVPFLPEAV
                     PGESRVRIPARGDKQTLVALSMRNANNYRAEQLRMMELRDKGVAKEQKTLKVLSALRD
                     DMHMKELPLHIECFDNSNLQGTHPVAACVVFRNGIPSKKDYRHFAIKGVEGPDDFASM
                     KEVVTRRYSRLLAENNPLPQLIVIDGGKGQLSAAYAALQSLGLEEIITAVGLAKRMEE
                     IYLATDKTPLFLDKNSSSLRLLMQLRNEAHRFGISFHRQKRSARFTRSALLDIPGLGP
                     RTLEKLLGHFGSLKQISLASQEELAKVVSFALALKITEWNRGK"
     gene            8183..9967
                     /locus_tag="PLM68_04470"
     CDS             8183..9967
                     /locus_tag="PLM68_04470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOT54652.1"
                     /translation="MKKLIETKDLRKVFGDKSVLGFFLAPALMKLMKLDKINKLYDKV
                     ADFEGRECLEAFLAEMRITYETPSSDLANLPKEGPFITVSNHAYGALDGIILMHLVSE
                     DRPDLKVIVNYLLSRIRPLQNFFLPVNAFDKNVSSRSSITGIRLAKESLQKGNPLGLF
                     PAGEVSHIGPSGLVEDIPWHTAIVKFIMNAGVPVVPIYFEGHNSARFLRRAKIHPMLQ
                     TASLPAEVCNKKGHRLVVRVGSPISVTEQQRYSDYKELGDYLRARTYALAANIENKDK
                     FKEPEHAAPIAPSKQHDLVTREIEQLPSRNCLFKINNFAAYYAPYSLIPNLMYEIGRK
                     REEAFRTVGEGTYQSIDLDTFDRNYHHLILWDTEKKMIVGAYRIGMGADLMKRFGVNG
                     FYNNTLFRFGKGFEPVLQQSMELGRAFISQEYRYETIPMMLLFKGLFHIMLRNEEYRY
                     FIGPVSISSWYPKLYQSLIYKYIMDHHSTLEYQGCIESRNPFSPDFGPVNPDVLLRDI
                     QTPEQIDRLLMRMSNRQYRLPSLVKRYLKLNSKIIVFNIDKEFNDSVDGFIVCDVLEI
                     PKEELMMVTKDISDLSIIENRFGKSVPR"
     gene            9975..10388
                     /locus_tag="PLM68_04475"
     CDS             9975..10388
                     /locus_tag="PLM68_04475"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOT54653.1"
                     /translation="MQKALEEAQVARRQEEIPVGAIIVCRGRIIARAHNMTETLNDPT
                     AHAEMQAITSACYTLGGKYLDQCTLYVTLEPCPMCAGALAWAQLGRLVYAASDPKRGF
                     TLVPGSLLHPKTKVTPGVLSAEAGELIKDFFAGKR"
     gene            10413..10483
                     /locus_tag="PLM68_04480"
     tRNA            10413..10483
                     /locus_tag="PLM68_04480"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10445..10447,aa:Gln,seq:ttg)
     gene            10490..10560
                     /locus_tag="PLM68_04485"
     tRNA            10490..10560
                     /locus_tag="PLM68_04485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10522..10524,aa:Gln,seq:ctg)
     gene            10588..>11873
                     /locus_tag="PLM68_04490"
     CDS             10588..>11873
                     /locus_tag="PLM68_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54654.1"
                     /translation="MNRLIAFLALIGCLAGPAITAHGQYYKTGEDPGRLKWNQVRTGP
                     INIIYPKGVDSLAFRYAWLLEQAAPHVISSLGTELRPIPIVIHPYNVNSNGMVVWAPK
                     RMELYTTPPSYGTRHNWEKQLVLHEARHVAQMQRAGENFFRVFGWLIGQQSEGVSVGL
                     YFPKWMLEGDASIAETSFSNAGRGREASFLMPYKAYFLEGKQFPYDKWRYGSYRHSMP
                     DHYALGYMKLSVARLFSGEEALGRIYEDITKYPFWPFIYGKTYRRNYGYSAIKLWKPA
                     AEYYKNIWKEQDALKTVTYSGEQINRPVKDYVSYRSAAVTTNPDGTRVLFSIKSSLAQ
                     TARLVRFPFSEKNREETVCLLGQVNSSLRASGPYLFWSETVSGPRWAHENFSVVILYN
                     TVTGEKKTLRHRTRYFNPCTSQNGSMLAVIHYPPGGG"
BASE COUNT         2914 a         3000 c         2824 g         3135 t
ORIGIN      
        1 gatccctccc ggtacaccat cctggccctg ttgccgtctt acctggaaag accgggatcg
       61 tccctggttt atatgatgca ggtgctcatg gaaaaaagcg gaactggagc aggcgggttt
      121 tatttatccg atcaggaagc cttatacggc aggctgctgg atctttctac ccagggaaag
      181 cctgtctggt tgataggggc agcatttgcc ctgcttgatt ttgttgataa attcaccctt
      241 cgtcaccccg gacttattgt agtggaaacc gggggcatga aaggcagggg aacagaactg
      301 acccgggaag cactccatga gcgtttgcgc cggggttttc cctatagtcc catccacagc
      361 gaatatggca tggccgagtt gttttcccag gcttatgcct gcacagatga tctttttttc
      421 agatgtcctc cttccatgca ggtcatgatc cggaacttgc aggatccgtt ttcaccggag
      481 cccgatggct ctgcaggcgg cattaacatc attgatacgg caaacagcca ttcatgttcg
      541 ttcatagaaa cagaagacct ggggttccgg attcccgggg gacaattcca ggttctggga
      601 aggatccccg acgcagaacg ccggggatgc aatatgctta ttgatatttc ttgaacaatt
      661 cttcaagctg ttgggtgtat gttcccgcaa gttctttgtc ctcgtttaca tccaggatgt
      721 ctttaacgtg aacatacatg ctgatggccg attcggcatc tgacggagac agcagtcctc
      781 ccctgaacga gcccatgaag tactcaatgg ctttgaaagt ttcatccagg aagggaacgg
      841 ccagagcact ggctttttcc ggtttgccta gaaaataata cagctctatc gtttccatga
      901 cacccacttc gttcatggaa ggctgcacca cccaattcaa aggatattgc ttcatgatat
      961 cgatcccctt gtccagcagg gcttcggcct tatcttcctt gccttccagg agcatggcct
     1021 tagcaacctg ggaatacatc tgccttatgg cctgctgcac caggtacaga taagtgacat
     1081 ggtaatccag caacacatcc ggctcatcca tcctgcccca ttggtaaatg tccatgagca
     1141 ggtggtacaa tttgtccgta tccatgtagg gcacctgggt agacaggcta tctaccttga
     1201 atggcatgag tttgtaagcc atcccgtcgt agcgcaaata atcctgtatc ccgatattca
     1261 ggtcaccacc cttggaaacg atgtatatgg gtctgtccca ctgataattg gccagaagat
     1321 ccaggaacat catttccact ttcatcaggg tattcttgtt ttccgggatg tccaggtcca
     1381 tgtaatccgg gataagatgc gcgtcctttt caggaacgat cccgcaggca agggcatttt
     1441 ccttgttgac cgggattctc agtttgcgtg caggcaggta attgagcatg gtcccgtcca
     1501 tgagctgcac cttggcacgg ggatttccca ttacgcctac tgcctgtatg gcagggaccc
     1561 tttcttttac cctttcatat acgggaatga tctcgttggt cccgtacaaa tatgattcac
     1621 gggggattga aaaagtaacc ggggcagatt catacattct cagctgcatc tggtcaatat
     1681 accagtctgt tcccagcagc gaagtgttta taacgcgcac atcggtcctg attccttcca
     1741 cttcctgtgc ataccacagg ggaaatgtgt cgttatcccc gtgcgtgact atgatggcat
     1801 tgcaatccgg cttgcaggtg ttcagaaaat taaaggcaaa ggcaggggct gtataccggc
     1861 cgctcctgtc atggtcatcc cagttttggg ccagcatctg gaatggtaca aacagggcga
     1921 gtgccgccgc cagggatccg gcaatggttt gggaaagttt cttttgaaac aggttaaaga
     1981 gggccattac ccccagccct gcccatatgg caaaggcata aaaagatccc gcgtaggcat
     2041 agtccctttc cctgggctgg tagggaggct ggttcagata aacaaggatg gcaagtcctg
     2101 tcatgaagaa cagcaggaag gtgatccagg cattgcggcg gtcttttaat gactgataga
     2161 taagtccaac gatacccagc aacaggggca gcatgtaata gtggtttttg cctttgttgt
     2221 ttttcagcat gtcaggggcc cccgactggt ctcccaggcg tgccttgtcc aggaatccta
     2281 taccgctttc ccagttgccg cgaatgggat cccccggaat ggtgccctgt atgtcgtttt
     2341 gtcgtccagc aaaattccac atgaaatagc gccagtacat ataattgacc tggtagtcaa
     2401 agaaaaaacg caggttgtcc ccaaaggtgg gcatgtgttt tccgttggaa ttcaatgtcc
     2461 cctttcccga gttgtatgag tcgtaaaagg cagcatggct ctcccgcttg ctccacattc
     2521 ggggaaacag cattttttcc gattccttgt atacaggcat ttccggtccc tttacccgtg
     2581 cgtagcgccc gttcattttt acatactgcc gggtatcctt ccaggtttcg tagtctgaat
     2641 tgtaaatccc gtcataaaac aagggggcag cgccgtattg ttcccgggcc agatatttcc
     2701 caagagaaaa aggattgtcg ggctggtttt cgttggtggg tgtattggca ctggcccgta
     2761 tcaccaccat ggcaaaggac gaatagccta tgatgatcac ggcaaggcat aataatatgg
     2821 tgttccacag cactttgcct tttttgtatg tggagaatac accccagaaa agggcagcga
     2881 taaggaccac cacaaaaaac agcatcccgg tgttgaaagg caatttgaat acattgacaa
     2941 acaacaggtc aaaccaaccc gatatttttg gcacccatgg aattattcca taaagaacga
     3001 ccgcaatgat caccacagag agcagaaggg taaccacggc tcctttccag gtaaacgggt
     3061 atttcttgta gaaatatata tagacaagtg ccgggatggt caacaaattc agcaggtgca
     3121 ccccaatgga aaggcccatc aggtaagcga tcagcacaat ccatcgattt gcatgcggct
     3181 tgtctgcatt ctcttcccat ttcagcatgg cccagaaaac tgccgccgta aagagcgatg
     3241 acatgccgta aacctcggcc tcaactgcag agaaccaggc tgtgtctgaa aaggaataga
     3301 tcagtgaacc cgcggctgcg gctccccata tggcaatggc attcccccgg ttcagggaaa
     3361 ttttctgtgt ttccagcagc cgtctaccca ggtgggaaat ggtccaaaat aaaaagagga
     3421 cgcaaaaagc cgaggccagg gcactcatgg cattgatgag cattgccgcc ttctggtcgc
     3481 tgccaagcat ggaaaacaac ctgccggcca actggtaagt cgggtttccg ggggggtgtc
     3541 ctatttccag tttataggaa gaggctatga attcgccgca atcccaaaaa ctggcggtgg
     3601 gttctatggt tgccaggtaa accgcggcag agatggcaaa gacgacccag ccaatcagaa
     3661 ggtccgcctt tttgaatgtc tttgtttcca ttccacaaat gtaggagatt ttttttacct
     3721 ttacctttgg atatgtttta aagtgatgga ttatattgtc ctggggttaa ttgtttggta
     3781 ttgtttcatc ctgtttttta tccggactcc tctcacactg ctgctgacag gttgtcttcg
     3841 ctacaacatt tccatagaga atgaggaaca ggtgaatctc aggttttcac gggttttcat
     3901 gtttttattc gtaataacat tagcctcgtt cttcatcgtt tcccatgaca cattgctttc
     3961 ccccctccct cacttcctgc tggggatgtc aaaaccaatg gttttccttt gcatcatggt
     4021 ctttttcatc gtgttgatcc ttgtcaggac tttttttaca aaaatcattg gctggacaat
     4081 tgattccagt gcgttcatga acttcctgaa ccgcacggcc tacgacttct gcattttgac
     4141 aggttttgtg ttcattccct tcctgatact gttctttgcc ctggggaatc cttccggcaa
     4201 cggtgtgata attgcagccg tggcaacagc tgcagccggt tatttggtct atgcataccg
     4261 ctgcataagg atttttcttt ctgccggttt ttctcttttt ttctggattt tgtatctttg
     4321 catctttgaa atgataccct tgggagtgtt aattcatttt gttgcaggga tacagacatg
     4381 aagatcaaga acatactcat tacacaaccg gcacctaacg gaggttctcc ttacctggaa
     4441 atcaaagaaa aatttaatct gaatattgat tttatccctt ttatccgtgt agaagggcta
     4501 tctgccagag atttcaggag ccagcgggtc aatttacagg atcattccgc cgttgtcttt
     4561 acaagccgtt ctgcaatcga ttcctttttc cacctatgcg aagagatgcg ccttaccatt
     4621 cctgagtccc tgaagtattt ctgcatgacg gaatccgtgg ctttttacct tcagaaatac
     4681 ataatataca ggaaaaggaa gatcttttac gggaaaggca atattccttc cttaatagaa
     4741 acaataggct ccaaacataa gaacgacacc ttcctgctta ccctggcaga ttgttataaa
     4801 ccggaacttc cgcaggcatt tgacaaggcg ggcctgaagt ataccaaagc catcctgacc
     4861 cataccatca tagaggatct gtcaccggtt gaattgaaaa aataccagat gatggtcttt
     4921 tattctccag gagacgtaag gtctctgttg gaaaatttcc cggacttcaa acaagatagc
     4981 gttttgttcg gcacctttgg tgccaatacg gctaccgctt tgaaagaagc taacctgcaa
     5041 gcctgttttg aagcacctac cccggaagcc ccttctgttg cccaggccct gatgctctac
     5101 ctggaaaagc acaagtgatc atgaaacgga caacctgtct gtgtatgggt tttcttctgg
     5161 ccatgatcac cttatcctgc cagaagaatt tcaaccatta cagggaactg gcctacggca
     5221 aatgggaact cgtctctgtt aacggagaat ctcccgttgc ggccgaacaa ctttatttca
     5281 agacaagcac ttcagtgact ctcacccggg aaggcgtctc ttacgagggg caataccagt
     5341 tttactgcaa tgtgcttacg acttattttg aacaatatca aaaccttttc caggagtggc
     5401 gcgtacagac catgaaggac tccctgatgc gtgccgtagt ggaattccgc gaaatgaaca
     5461 gcgacacttt ggatgcagga agtgtcctgg aatacagaaa aattccgtga tgaaaacggg
     5521 atcaacatat gcgtttccca aaaaaatgcg gttgtcttcc cggaaagaga tagaatccct
     5581 ttttacagat gcctgttctt ttaccacggg tcctttccgt ctgttttaca ggacctgtga
     5641 cgtgcacgaa cccgaccagc cgaaaacgga cctgccggaa acagaaaagg gtggttgcag
     5701 cctggtcttt tccgttccca agaaaaacct gaaatccgct gtaaagagaa atctgataaa
     5761 aaggcgcctc cgggaatctt tccggcttca ttatccccgt ttgctgaaac cggttcttga
     5821 agcaacaggc acaaggcttg tctttttatg tatatattta ccccatgaag tcagaccatt
     5881 ctcatacgtg gaaacaaaaa tgcaggtttt actcgggcat tttgctcaat tgtctgaaaa
     5941 aggttctgac ttttcccctg atcctgctgg ttaaatgcta ccagttgtgt atctcgccct
     6001 ttacacctcc ttcctgccgt tttatcccca cttgttccca gtatgcactg gaagccctgc
     6061 gcaggcacgg tcctctgaaa ggcctatacc tggctatccg gcgttttctc cgctgcaatc
     6121 cctggggcgg cagcgggtat gaccccgtcc cctgagtcct gagccctgtt ttctgtcttc
     6181 agtcttctgt ttgctgcggt cttgccttct gtttgctgcc tctgctgcgg tcgctggcac
     6241 cacaagaacg ggaagctttt tctttaagcc gaggcttccc ttatcttttg gtgccagcga
     6301 ccgccttctg gcagcaaaat ttgagtgccc cgggttactt tccacggttc cactctgtta
     6361 tcttgagagc cagtgcaaag gaaacaacct ttgccagctc ttcctgtgat gccagggaaa
     6421 tttgtttgag acttccgaaa tgacccagca gtttttccag ggtacgtggt ccgagcccgg
     6481 ggatatccaa cagggctgac cttgtgaaac gggcacttct tttttgcctg tggaacgaga
     6541 tgccgaaacg gtgtgcctcg ttgcgcaatt gcatgagcaa ccgcagggaa gaagaattct
     6601 tgtccaggaa taatggtgtt ttatctgtgg ccaggtaaat ctcctccatg cgtttagcca
     6661 ggcctacagc cgttatgatc tcctccagcc ccaggctttg caatgcagca taagccgccg
     6721 acaattgtcc cttgcccccg tctattacaa tgagttgtgg caggggattg ttctctgcca
     6781 gaagacggct gtaccggcgc gttaccactt ccttcataga ggcaaagtca tcaggaccct
     6841 caacgccttt tatggcaaag tgacggtaat cctttttgct ggggatcccg ttgcgaaaca
     6901 ccacacaggc agccacggga tgagtcccct gcaggtttga attatcaaaa cactctatgt
     6961 gcaagggcaa ctctttcatg tgcatatcgt cccgcaaggc agagagaacc ttcagggttt
     7021 tttgttcctt cgctaccccc ttgtctctca actccatcat gcgtagctgc tctgccctgt
     7081 aattatttgc attccgcatg ctcagggcaa caagggtttg cttgtccccc ctggccggaa
     7141 tgcggacccg gctttctccg ggaacggctt cgggaagaaa gggaaccagg atttctgcag
     7201 acaattgtcc cagttgttct ttcatttcag ccatgaacaa actcaacacg gctgcaggtt
     7261 cttcttctat atgcagtttg tatttcacat tcagcgattg tatgaccgca ccgtcggcaa
     7321 cccgcatgaa attaccgaaa gccatggagg acccggcatc ggttgtaagg ctgaaaacgt
     7381 caaggttgga tatccgggaa ttcacaatga ccgattttgc ctggtagcgg gtaagcattt
     7441 cccactgttc cttgtatctc tgggcaagtt caaaattgaa tgcctgggaa gccgccttca
     7501 tctggctttc cagcagtttt cgcacagttg tgacatctcc tttcaggatg ctggacaccg
     7561 agctgatgta ctcctggtat tgttcacttg tctcttcccc tgtacagggg gctttgcaac
     7621 ggcctatgtg tgcctggagg cattttctgt accgcccggc agtcacagcc tgcggggcca
     7681 ggggcagcga acacgtccgc agcggataga gattgtgaag gagttgcagg agttgtttgc
     7741 aataaaagcc gctggtgtaa gggccgaaat aataagaacc gtcccgcacg atcctccttg
     7801 tctggaaaac acgtggaaaa ggttcgtttt tcacacaaat ccagggaaag gtcttgtcat
     7861 ccttcagcaa gatattgtaa cggggctgta tggtcttgat aaggttgttc tccagcaaca
     7921 gcgcatcgct ttcattgtcc accaccgtat attccagcga ctgtacctgg ctgaccatgc
     7981 gtttggtttt tgcagaaagg ttcctgccgg gggcaaaata ctgggaaaca cgttttttaa
     8041 gatcttttgc cttacccaca tagatcactt ttccttccct gttaaggaat ctatacactc
     8101 cgcactgatg cggcaacaga gccagttggt cttcctcttg aatcatttct aggtaaaaat
     8161 acatatattt gttaaaccat ttatgaaaaa actgatagaa acaaaagatc tgcgtaaggt
     8221 tttcggggat aaatcggttc tgggtttttt tctggctccg gccctgatga agctcatgaa
     8281 acttgacaaa atcaacaagc tttatgataa ggtcgcagat tttgagggcc gtgagtgcct
     8341 ggaggctttt cttgccgaga tgcggataac atatgagact ccttcctctg acctggcaaa
     8401 ccttcccaaa gaaggtccat ttataaccgt gtccaaccac gcttacggcg ccctggacgg
     8461 aatcatcctg atgcatcttg tatcggagga tcggcccgat ttgaaggtta tcgtgaatta
     8521 cctgttatcg cgcatcaggc cgttgcagaa ttttttcttg cctgtgaatg cttttgataa
     8581 aaatgtatct tcccgctcca gcataacagg aatacgcctg gcaaaggaaa gtcttcaaaa
     8641 aggcaatccc ctgggtctgt tccctgccgg agaagtatct cacattggac cttccggact
     8701 ggttgaagat attccctggc acaccgccat tgtcaaattc atcatgaacg caggggttcc
     8761 cgttgttccg atttattttg aaggacataa ttcggcgcgt ttcctgcgca gggcaaaaat
     8821 acatcccatg ttgcagactg ccagtcttcc ggctgaagtg tgcaataaaa aaggacaccg
     8881 cctggttgtt cgtgtgggat cccccattag cgtgacagaa caacagcgtt attcagacta
     8941 caaggaactg ggagattacc tgcgcgcccg cacgtatgcc ctggcggcaa acatcgagaa
     9001 taaagacaaa ttcaaggagc cggaacacgc cgctcccatt gccccgtcaa aacaacacga
     9061 cctcgttacc agggagattg aacagctgcc ctcccgaaat tgtctcttca aaatcaacaa
     9121 ttttgcggct tattatgccc catattcact cattcccaac ctcatgtatg agattggccg
     9181 caaacgggaa gaagccttcc gcaccgttgg cgaaggcaca taccagtcaa ttgacctgga
     9241 tacctttgac agaaattacc accatctgat cctttgggat acagagaaaa aaatgatcgt
     9301 aggggcttac cgcataggca tgggcgccga cctgatgaaa cggttcgggg taaacgggtt
     9361 ttacaataat acactcttca ggttcggaaa gggttttgaa ccggtcctgc aacagagcat
     9421 ggaactggga agggctttca taagccagga atatcgttac gagaccattc ccatgatgct
     9481 gcttttcaaa ggtctcttcc atatcatgct ccgcaatgaa gaataccgct atttcatagg
     9541 acccgtgagc atatcctcgt ggtatccaaa attgtatcaa tccctgatct acaaatacat
     9601 catggatcat cacagtactt tggaatacca gggctgcatc gaatccagga atcctttctc
     9661 cccggatttc ggacctgtca atccggatgt cctcctgcgc gatatccaga caccggaaca
     9721 gattgaccgg ctgctgatgc gtatgagcaa ccgtcaatac cgcctgcctt cactcgtcaa
     9781 aagatacctg aaactgaatt ccaagatcat tgtattcaac attgacaaag aattcaatga
     9841 ctctgtggac ggtttcatcg tgtgcgatgt ccttgaaatt cctaaagagg aactgatgat
     9901 ggttaccaag gatatctctg atctttccat aatagaaaac cgttttggaa agtccgttcc
     9961 aagataggac gttcatgcag aaagcgcttg aagaggcgca ggtcgcccgc cggcaggagg
    10021 aaattcctgt gggcgcgatc attgtatgtc gcggcaggat cattgccagg gctcataaca
    10081 tgacagaaac cttgaatgat cccacggcgc atgcggaaat gcaggccatc acatccgcct
    10141 gctatacatt gggtggaaaa tacctggacc agtgcaccct gtatgtaacc ctcgaaccct
    10201 gccccatgtg cgccggggca ctggcctggg cacagcttgg acgcctggtc tatgccgctt
    10261 ccgaccccaa aaggggattc acccttgtgc caggcagtct tctccatccg aaaacaaaag
    10321 tgacgcccgg tgtactgtcc gcagaggccg gagagctgat aaaagatttt tttgccggaa
    10381 aacgctaatg ttatatcttt gcaccttccg attgagctat ggtgtaatgg tagcactaca
    10441 gattttggtt ctgtctgccc aggttcgaat cctggtagct caacaacagt gagctatggt
    10501 gtaatggtag cactacagat tctggctctg tcggtccggg ttcgagtcct ggtagctcaa
    10561 ctcttaacaa tcagttaact atacataatg aacagactga ttgcctttct tgccctgata
    10621 gggtgccttg ccggacccgc catcaccgca catggtcaat actacaaaac aggtgaagat
    10681 cctggccgac tgaaatggaa ccaggtccgt acaggtccca tcaacatcat ctatcccaaa
    10741 ggagttgact cactggcttt ccgttatgcc tggctgctgg aacaggctgc tccccatgta
    10801 atatcttccc tggggaccga attacgtccc attcccatag tcatccaccc ttataatgta
    10861 aacagcaacg gcatggtggt ctgggccccc aagaggatgg aactgtacac cactccccct
    10921 tcctacggaa caaggcataa ctgggaaaaa caactcgtat tgcatgaagc cagacacgtg
    10981 gcacaaatgc aacgggccgg tgagaatttc ttccgtgttt tcggctggct catcgggcaa
    11041 cagtcggaag gtgtttctgt agggctttat ttcccaaagt ggatgctgga aggagacgcc
    11101 agtattgcag aaacatcttt ttccaatgcg ggacgcggca gggaagcctc attccttatg
    11161 ccctacaaag cctattttct ggaggggaaa caatttcctt atgataaatg gcggtatggt
    11221 tcttaccgtc attcgatgcc ggatcattac gcactgggtt acatgaaact ttcggttgcc
    11281 aggttgtttt caggcgaaga agccctgggg cgcatttatg aggatattac aaaataccct
    11341 ttctggcctt ttatctatgg gaaaacatac cgcagaaact acggttattc tgctattaaa
    11401 ctatggaaac ctgcggccga gtactataaa aatatctgga aggaacagga cgcgctgaaa
    11461 accgtcacat attccggaga acagatcaac cgccccgtaa aggattatgt gagttaccgc
    11521 tcagccgcag tgacaacgaa ccccgatgga acccgggttt tgttctccat caaatcttcc
    11581 ctggcccaga ctgcccgact ggtgcgtttc cccttttcag aaaagaaccg ggaggaaacc
    11641 gtgtgcctgc tgggccaggt caattcttca ttgcgtgctt cggggccgta tttattctgg
    11701 agtgaaacgg tgagcggccc ccgttgggct catgagaatt tttctgttgt catcctctac
    11761 aataccgtga caggagaaaa aaagaccctc cggcaccgga cccggtattt caatccgtgt
    11821 accagccaga acgggagcat gctggcagtg atccattacc ctcccggggg cgg
//
LOCUS       DAOSOF010000055        11155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5824_length_11155_cov_5.671171, whole genome shotgun sequence.
ACCESSION   DAOSOF010000055 DAOSOF010000000
VERSION     DAOSOF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_11155_cov_5.671171"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLM68_04495"
     CDS             complement(<1..559)
                     /locus_tag="PLM68_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54655.1"
                     /translation="MSENLFEKKIREKLYDFSIPGETGDWEALAARMSGRKAFLRRRR
                     VLILIPAAAACLLLLMFLLMPVTDRKSLAPVTVCHIVPQPASLELAVAPGDIPALLSQ
                     QGLELLRSRSVIPATSEITTPADMIPSPEIQQATPPQVAMEEPAVEKVMPVEIPPKAA
                     ATKTNGLYPDGIPKNPRLNRKWITAA"
     gene            complement(552..1019)
                     /locus_tag="PLM68_04500"
     CDS             complement(552..1019)
                     /locus_tag="PLM68_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54656.1"
                     /translation="MKRIQFISFIIITVITASCRDSIPFRYDPSEPDSLSTRRYPHAF
                     FIVRGEIVDSLTQEPIPGLTVNLRQEDTVLTDSNGVYLAQTVAFPISQEFRMILNTYG
                     EQHNPLYLPETLSVIFSMPTFKLSQDEILEHGPQFFGSAYVTINRTLIPLVHE"
     gene            complement(1016..1582)
                     /locus_tag="PLM68_04505"
     CDS             complement(1016..1582)
                     /locus_tag="PLM68_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54657.1"
                     /translation="MQHSGRDFHLNKDIPESDLIAACKKNDATAQRVIYERLYSRMLA
                     VCQRYTGNRETARDVVQDGFVVLFDKIGTYAGTGSFEGWARKIFVNMSLSHLRKNDAL
                     KYTEPIENTAAKTVTTGLILENISAGDILHCMEQMPYGFRTIFNMYAIEGYSHNEIAK
                     LLDISESTSRSQYSRARSWLQNKLNELK"
     gene            1680..3365
                     /locus_tag="PLM68_04510"
     CDS             1680..3365
                     /locus_tag="PLM68_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54658.1"
                     /translation="MCKRVCVLVLLVVLTSCGYRAEMATEESSVLFYQGLKMFYLGEF
                     PQAREYFKRTVSRDRKNDAAFYYLAQIAGYAGQWEESGEYLDKALKLDPGNYWYRLQQ
                     AKLAIASGKEEEAGSLYENLWLDYPAKTELLYDLIGLYMNGRKTDKALKLLDTLEARE
                     GVTESGLLLRFNLMMETDKEAAQTLLRAYADHTPSPRVLSVLGDLAGGDGEIDKAGDY
                     YRRALEADPAFMPAIFGIAETYRMKRQFDLFFENINIFLAYPDVDVRMKTDYLKQILD
                     TRGFVATFQPQVDTLFLSARSAHKTDSTLAYMYGGYLIQTEKKAEALEVIKDNLRYYP
                     NDFHAWYQTLGVIYYLKEWPLLCEYGSRALEIFPDNPDFSSLYGLGLWQTGQLDQAIA
                     RFERILSLLKKKDMTNKVQTMALLGDLYHEKGDWERSFRMYDEVLRLDRENLAVLNNY
                     AYYLSLREEELEKALEMSQVTIDKEPKNPTYLDTYAWILHKLGRSAEARNIFRQALAY
                     GGKESAEILDHYGDVLNALNEPLMAIVYWQQAYDIEPRQDILEKIETNKKNGQ"
     gene            3369..4541
                     /locus_tag="PLM68_04515"
     CDS             3369..4541
                     /locus_tag="PLM68_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT54659.1"
                     /translation="MKRVLLILSILLAAGARAGAQDVGALQSRNNRLREEIATLESRI
                     DSTTDNIRGQLTQYRLGQSLIASRQELLRGMEQELAVLNSRIAEKNQMILRLRQEHNH
                     LLTAYESLLQQAYRHRDRKLWIMHILSSESASQAYRRWRYFRNYSDYMRTQEQKIRET
                     QDSLVREETRLVSLQQERVQARDDVALEVSRMEKDQRLLDGNIKKMQQNEKQLKAALQ
                     RRIEEQRKLDREIREALAALEKKDIATDPAAVEAARVLSEDFAANKGNLPWPLKNAVV
                     IERFGRITDPKWGFTFDNKGVTLSGTKAGEVRVVFNGTVSSIWMDNRDSYLIRILVTH
                     GQYSTIYCHMVDVVVKEGQKVVTGQKLGTLSPQGEGNSYFQLLHKGIPVNPADWCR"
     gene            complement(4516..6132)
                     /locus_tag="PLM68_04520"
     CDS             complement(4516..6132)
                     /locus_tag="PLM68_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOT54660.1"
                     /translation="MKSLQEINKENNGPDAGRYLAALVDNRLKSLDYILYNPHNVQFV
                     DYSHPDGRRTYIRSLSFVLQKAVKEVFPEARLRISYSVVNSLYCNLDLPDGREETNRD
                     MELICQAMNRIIEQDIPFRMEKVRSEEAQQIFEKAGFPQKALLQKTRGHFFTSVYYLN
                     DSPDTFYGPLVPSTGYLKLFSLRRFYKGFLLQYPQPDNPLQLDGPPQLHKLVEVFEEY
                     VNWGNIMGVKDVGSLNQTIQRGETKDLILIGEALHSRRYATIADTIFGLRDKVRLVLI
                     AGPSSSGKTTTSYRISKQLKVLGLQPVILEMDNYFVDREDSPRDENGEYDFESVHAMD
                     MAFLNRQLFELLEGKEVEIPFFNFLTGRRFFNGRKIKLEDNQILIMEGIHALNPDLTK
                     DIPEENKFRIYASALTSLAMDENNRISTTDTRMIRRMVRDAQFRGISAEETILRWHSI
                     SYGERQNIFPYQEEADVMFNSALPYELAVLKSHAEPLLHRIPPVSEAYPEALRLLNFL
                     SYFSAVHPRDERYIPYVSVIREFIGTNQQD"
     gene            complement(6145..8979)
                     /gene="uvrA"
                     /locus_tag="PLM68_04525"
     CDS             complement(6145..8979)
                     /gene="uvrA"
                     /locus_tag="PLM68_04525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT54661.1"
                     /translation="MLIHMPASHIRIEGARVNNLKNISLDIPGHTLCIITGLSGSGKS
                     SLAFDTLHAEGQRRYLETLSSYARHYLGILQRPDVEHISGLSPIIAIEQKTTTRNPRS
                     TVGTVTEIYDFLRLLFARASIAYSPVTGKPMIRYSAEQMVSLIATQYQGRKCILLAPV
                     VKGRKGHYRELLDQILKKGFLEVRIDGSIEQIGHNMMLDRYKTHFIEIVIDKLIPSGQ
                     DNASLARLRDSVNMALHQGNGSFAVLDPDTGNITHYSKHLMCPESGLSFPEPAPFSFS
                     FNSPLGACPKCKGLGFTTCIDLKKIIPDNSLSIAQGGMEPFGKLRNNTLFAHLEGVAK
                     KYGFSLDTPIAEIPEEAMDTLLYGSEDLIYVHSEMGGPYTITFPGLINRMEQVNGEEE
                     EKMATRERYLEEVMCPVCHGSRLNQDALCFRFAGKNIAELAAMDIQPLVTWLEGVEDR
                     IPERQQAIAADILKEIRDRLQFLLDVGLSYLSLDRGTRTLSGGESQRIRLATQIGSTM
                     VNVLYILDEPSIGLHQRDNRRLINALLKLRDAGNSVLVVEHDQETILSADWLVDLGPG
                     AGREGGNLLYSGPMNKFLEEPHQDSLTWQYLKGIRSIPVPGTRRTGNGHVLRVSEATG
                     NNLKNITVDFPLGCFICVTGVSGSGKSSLVNETLVPVLTRKFYRSKRPFLPHRSVTGI
                     QHIDKVVEVDQSPIGKTPRSNPATYTNVFADIRKLFAASQDARIRGFKSSRFSFNIRG
                     GRCETCKGAGIQLLEMNFLPDVHITCPDCQGKRYKSDTLAVRYKNKNIYEVLDMTVNE
                     AVDFFRHVPSIIQKIKTLQDVGLGYIKLGQPSTTLSGGECQRVKLAAELSRISTGNTL
                     YVLDEPTTGLHFEDVKVLLHILQQLTDKGNTIVIIEHHPDIILAADHIIDLGPEGGDG
                     GGYVVGVGTPEELAENLQSYTGVLIRKWLK"
     gene            complement(8973..9617)
                     /locus_tag="PLM68_04530"
     CDS             complement(8973..9617)
                     /locus_tag="PLM68_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT54662.1"
                     /translation="MEIKTFYFNALHTCCYVAYDQTGNCIIIDPGCYRQREEQRLTAF
                     IKSQNLQPRYIVNTHCHFDHLMGLGFLQKTYDVPFLAHPLEAEILKRSISQALLFGFN
                     MTPHGTDFTPLADGQVLSFGTSSLKVLHTPGHSPGSVCLYGNLEGILFTGDLLFAGSV
                     GRTDLPGGDFDQLMDSFVNKIRPLPRFTLVFPGHGPASSLEREFADNPYLQHIC"
     gene            complement(9607..10266)
                     /locus_tag="PLM68_04535"
     CDS             complement(9607..10266)
                     /locus_tag="PLM68_04535"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT54663.1"
                     /translation="MSDTLRQKGQRRLLVEELARKFAFNPLVLKAMEQVPRHLFVEKG
                     LEHMAYNDKPLPITGSQTISQPYTVAMQTHLLNGSKMDKVLEIGTGCGYQAAILMAMG
                     YRVYSIERIWELYLQAQKNLVATGFDPSGLYYGDGFAGLPQFAPFKGILVTCAAPSVP
                     EKLKEQLSINGRLVIPVGRYEQKMLRITRTGEDTYSTEDFGTYSFVPMLTGTQKIKHG
                     D"
     gene            complement(10344..>11155)
                     /locus_tag="PLM68_04540"
     CDS             complement(10344..>11155)
                     /locus_tag="PLM68_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54664.1"
                     /translation="ESNVSNKDQLAEKFLRLASNPVEAERQIRAEIASNAEVKNVMMP
                     LLRYGSVAVNFDPIQLTAEEIRVIADNQPEYLTPNDIIQAATDLEPDEAIALYKRSLD
                     RYPDVVELYVNLAYYQFWNNDLEGARQTLENAKLVADEPAEKDIVALQCACVAMKAGE
                     YSQAESALNSISDEGMTRYYRGVLAIYQQDNDKALRLLEDSKDINYAIALLNKNKVKE
                     ALNVLKDLDQGFPYVLYSTGVAYGRLNENAKAAEYKAKAYQIDPSLRLLDK"
BASE COUNT         2556 a         2807 c         3099 g         2693 t
ORIGIN      
        1 tggcggcagt tatccatttt ctattcagcc tgggattttt tgggatgcca tccgggtaca
       61 agccattggt tttcgttgca gcggcttttg gcgggatttc cacgggcata accttctcaa
      121 cagcaggctc ttccatggcc acctgtggtg gggtagcctg ctgaatttcc ggggaaggga
      181 tcatgtcagc aggagtggtt atctcggagg tggcaggaat aacggaacgg ctgcgcagca
      241 gttccaggcc ctgctgcgag agtaatgcgg ggatgtctcc cggagcgacg gcaagctcaa
      301 gggaggcagg ttgaggtacg atatggcaaa cggttacggg agcaagagac ttcctgtctg
      361 tgacaggcat gagtaaaaac atgagcagca acaggcaggc ggcagctgca gggatgagga
      421 tcaaaaccct tctgcggcgc aggaatgctt tgcgccccga catgcgtgcc gccagggctt
      481 cccagtcccc ggtttccccg gggatggaga aatcgtacag cttctcccgg attttctttt
      541 caaacaaatt ttcactcatg aaccaaaggg attaatgttc tgtttattgt cacatacgca
      601 ctgccaaaaa actgcggtcc gtgttccagg atctcatcct ggctcagctt gaaagtaggc
      661 atggaaaaaa tgacggatag cgtttcgggg agatacagag gattgtgttg ttccccatag
      721 gtgttcagta tcatccggaa ttcctgtgat atgggaaagg caacggtttg tgccagatac
      781 acgccgtttg aatcggtaag gaccgtgtct tcctggcgta ggttcacggt aagtccaggg
      841 ataggttcct gtgtcaggga atcgacgatc tctccccgga ctatgaaaaa ggcatgcgga
      901 tacctgcgtg tggacaggga gtccggctct gacggatcat aacggaaagg aatgctgtcc
      961 ctgcaagagg cagtgatcac cgtgataatg atgaaactta tgaactgtat ccgtttcatt
     1021 ttagttcgtt cagtttattc tgcagccatg acctggccct ggaatactgg gagcgggaag
     1081 tactttcact aatgtccaac aacttggcta tttcgttatg tgagtaccct tcaatggcat
     1141 acatgttaaa aatggtacgg aaaccgtaag gcatctgttc catgcagtga aggatgtcac
     1201 cggccgatat gttttccagg atcaatccgg ttgtaaccgt tttggcggct gtgttctcta
     1261 tgggctccgt gtatttcagg gcatcgtttt tgcgaaggtg ggaaagggac atgttcacaa
     1321 aaattttccg tgcccacccc tcaaaagagc ctgttcccgc ataagtcccg atcttgtcaa
     1381 acaacacgac aaatccatcc tgtaccacat ccctggcggt ctcccggttg cctgtgtagc
     1441 gttgacagac cgccagcatt ctggaataga gacgctcata aatgacacgt tgtgccgtcg
     1501 catcattctt tttgcaggca gctatgaggt cactttccgg aatgtcttta tttagatgaa
     1561 aatcacggcc ggaatgttgc atggatatga gaaaattcta caggctaaac aaatgtaaaa
     1621 taaataattg aattgaacga attatggtaa ctttgcaact cctgtaatac tttgaaccca
     1681 tgtgcaagcg tgtttgtgtc ctcgtactcc tggttgtttt gacatcttgc ggttaccgtg
     1741 ccgagatggc taccgaagaa tccagtgtct tattttacca gggattgaaa atgttttacc
     1801 tgggagaatt tccgcaggcc agggaatatt tcaagcgtac cgtatcccgc gacaggaaaa
     1861 acgatgcggc tttttactac ctggcacaga tagccggtta tgcaggacaa tgggaagaat
     1921 cgggggaata cctggataaa gccctgaagc tggacccggg aaattactgg taccgcctgc
     1981 agcaggcaaa gctggccata gcttccggca aagaggaaga agccggatcg ctttacgaaa
     2041 acctgtggct ggattacccg gccaaaacag aactgctgta cgatctcata ggtttgtaca
     2101 tgaacggccg caagacagac aaagcgctga agttgctcga taccctggag gccagggaag
     2161 gtgtgaccga gtctggactg ctgctccgct tcaacttgat gatggagacc gataaggaag
     2221 ccgcccagac cctgttgcgc gcttatgctg accatacacc ctctccccgt gttctcagcg
     2281 tcctgggaga cctggcaggt ggagacgggg agattgataa ggccggtgat tattaccgga
     2341 gggcactgga agcagaccct gccttcatgc cggcaatttt tggcattgca gaaacatacc
     2401 gcatgaagcg gcagtttgac ctgtttttcg agaacatcaa cattttcctg gcctatcccg
     2461 atgttgacgt gcgcatgaag acagattacc tgaagcagat cctggatacc aggggctttg
     2521 ttgccacttt ccagccgcag gtggacaccc tgttcctgag tgcccgaagt gcgcacaaaa
     2581 cagacagcac cctggcttac atgtatggcg gctatcttat ccagacagaa aaaaaagccg
     2641 aggcgctgga ggtgatcaaa gataacctcc ggtattatcc caacgatttc cacgcctggt
     2701 accagaccct gggagtgatc tattacctga aggaatggcc cctgttgtgc gaatacggca
     2761 gtcgtgcact ggaaattttt ccggacaatc ccgacttcag cagcctttac gggctgggat
     2821 tatggcagac cggacagctg gaccaggcca tagcccggtt tgagcggatc ctgtccctgc
     2881 tgaaaaagaa agacatgaca aacaaggtgc agaccatggc cctgctggga gacctgtacc
     2941 atgaaaaagg agattgggag cgctctttcc gaatgtatga cgaagtgctc cggctggaca
     3001 gggaaaacct ggcggtcctg aacaactatg cctattatct ttccctgcgg gaagaggaac
     3061 tggagaaggc cctggaaatg agccaggtca ccattgacaa ggaaccgaaa aacccgacct
     3121 acctggacac atacgcctgg atcctgcata agctgggccg ttctgccgag gcaaggaata
     3181 ttttcagaca ggccctggca tacggaggca aggaaagcgc agagatactg gaccactacg
     3241 gagatgttct caatgccctg aacgaaccgc tcatggccat tgtgtactgg caacaggctt
     3301 acgacattga accccgacag gacatcctgg aaaaaataga gacaaacaaa aaaaacgggc
     3361 aataggccat gaagcgggtg ttgcttatcc tttccattct cctggcagca ggcgcaaggg
     3421 ccggggcaca ggatgtgggg gcactgcaat cccgcaataa caggctccgg gaagagatag
     3481 ccacactcga aagcaggatc gactccacca cagacaacat ccggggtcag ctaacgcaat
     3541 acaggctggg gcaaagcctt attgccagca ggcaggaact gctcaggggc atggagcagg
     3601 aactggccgt gctcaacagc cggattgcgg aaaaaaacca aatgatctta cggctcaggc
     3661 aggaacacaa ccacctgctc acggcctatg aatccttatt gcaacaggca tacaggcacc
     3721 gtgaccggaa actctggatc atgcacatcc tttccagtga aagcgcctca caggcctatc
     3781 gcaggtggcg ttatttccgc aattacagcg actacatgcg tacgcaggaa cagaagataa
     3841 gggaaacgca ggacagcctg gtccgggaag agacacgcct ggtatccctg cagcaggaac
     3901 gtgtacaggc ccgggacgac gtggccctgg aagtatcacg catggaaaag gaccagcgcc
     3961 ttcttgacgg caacatcaag aagatgcagc agaatgaaaa gcaacttaag gcagccttgc
     4021 agaggcgcat agaggaacag cgcaaactgg acagggagat ccgggaagcg ctggctgcgc
     4081 tggaaaagaa ggacatagcc acagatccgg cggccgttga ggcggcgcgc gtcctgtcgg
     4141 aagattttgc ggccaacaag gggaacttgc cgtggccgct gaaaaatgct gttgtcattg
     4201 agcgttttgg acgtatcacg gacccaaaat ggggctttac cttcgataac aagggcgtta
     4261 ccctgtcggg caccaaagcg ggggaagtca gggtcgtctt caacgggacc gtgtccagta
     4321 tctggatgga caaccgcgac agttacctga tacggatact ggtcacacac ggccagtaca
     4381 gcaccatcta ttgccacatg gtggatgtgg ttgtcaagga agggcaaaag gtggtaacgg
     4441 gacagaaact ggggacgctc tctccccagg gggaagggaa cagttacttt caactgcttc
     4501 acaaagggat tcccgtcaat cctgctgatt ggtgccgata aactccctga tcacagacac
     4561 ataaggaata tagcgctcat ccctggggtg aacggcggag aaataactca aaaagttcag
     4621 cagccgcagg gcttcggggt atgcctcgga aacggggggg atccggtgca gcaaaggctc
     4681 ggcgtgggat ttaaggacag ccagctcata tggcagggcc gagttgaaca tgacatcggc
     4741 ctcttcctgg tatgggaaga tgttctgacg ctccccgtag ctgatactgt gccagcggag
     4801 gatggtctct tcggccgaaa tgcccctgaa ttgcgcatcg cgcaccatcc tgcgtatcat
     4861 ccgggtgtcg gtggtggaga tgcggttgtt ctcgtccatg gccagggagg tcagggccga
     4921 ggcataaatg cggaacttat tctcttctgg gatgtccttt gtcaggtccg ggttcagggc
     4981 atggatccct tccatgatca ggatttggtt atcttccagc ttgattttcc ttccgttgaa
     5041 gaaacgcctg ccggtcagaa aattgaaaaa ggggatctcc acttccttgc cttccagcag
     5101 ttcaaacagt tgcctgttca ggaatgccat atccatggcg tgaacggatt caaaatcgta
     5161 ctctccgttt tcatcgcgcg gggaatcttc ccggtcaacg aaataattgt ccatttccag
     5221 gataacaggc tgcaggccca gcaccttcag ttgtttggaa atcctgtagg atgtggtggt
     5281 ctttccgctg ctggagggac ccgcaatgag taccagcctg actttgtccc ttagtccgaa
     5341 gatggtgtca gcgatggtgg cataccgtct gctgtgcagc gcttctccaa tcagtatcaa
     5401 gtcttttgtc tccccgcgct gtatggtctg gttgaggctg ccaacatctt tcacccccat
     5461 gatattgccc cagttgacat attcctcgaa aacttccacc agcttgtgca gctgcggggg
     5521 gccgtcaagc tgcaggggat tgtccggttg cgggtactgg agcaggaaac ccttgtaaaa
     5581 acggcgcagg ctgaagagtt tcaggtagcc cgtggatggc accagcggac catagaaggt
     5641 gtccgggctg tcattcaggt aatagacgga agtgaagaaa tggccccgcg ttttctgaag
     5701 caacgctttt tggggaaaac ctgctttctc gaatatttgc tgcgcttctt cagaccggac
     5761 cttttccatt ctgaatggaa tgtcctgttc aatgatccgg ttcatagcct ggcagatcag
     5821 ttccatgtcc cggttggtct cctctcttcc atcgggcagg tccaggttgc aatagagcga
     5881 attcaccacg gaataggata tcctcagcct ggcttcgggg aagacttctt ttacggcttt
     5941 ctgcaggaca aaggaaagag aacggatata ggtccttctt ccgtcgggat gtgaataatc
     6001 cacgaattgc acattatgtg gattgtacag aatatagtcc aggcttttga ggcggttgtc
     6061 caccagggct gccaggtatc tcccggcatc gggcccgtta ttttccttgt tgatctcttg
     6121 caagcttttc ataggcgatt ctctttattt tagccatttc ctgatcagga cgcctgtata
     6181 ggactgcaaa ttctctgcca gttcttccgg cgttccgact cccacaacgt atcctccgcc
     6241 atcccctcct tccggaccca ggtcaatgat gtggtcggcc gccaggatga tgtccggatg
     6301 gtgctcaatg atgacaatgg tgttgccttt gtcggtaagc tgttgcagga tatggaggag
     6361 gaccttcacg tcctcgaaat gcaaaccggt ggtaggctca tccagaacat agagcgtatt
     6421 gcccgtggag atgcgggaca attcggctgc cagttttacg cgctggcatt cacccccgct
     6481 aagggtggtg gagggttgtc ccagttttat gtatcccagg cccacatcct gcagggtttt
     6541 gattttctgg atgatggaag gaacatgcct gaaaaagtca acggcttcgt tgacagtcat
     6601 gtccagcact tcgtagatgt tcttgttctt gtagcgaacg gccagggtgt cgcttttgta
     6661 acgttttccc tggcagtcgg gacaagtgat gtgtacgtcc ggcaggaaat tcatttccag
     6721 gagctgtata cccgccccct tgcaggtttc gcaccgcccg cccctgatat tgaaggaaaa
     6781 gcgggaagac ttgaaccccc gtatcctggc atcctgtgaa gcggcaaaga gcttgcggat
     6841 atcggcaaac acgttggtat aggttgcggg attgctgcgg ggcgttttcc caatgggcga
     6901 ctggtccact tccaccacct tgtctatgtg ctggatgccg gtcaccgacc tgtggggcag
     6961 gaaaggtctt ttggaacggt aaaacttcct ggtcagcaca ggcacgagcg tttcgtttac
     7021 caggctggac ttcccgctgc cgctcacacc cgtgacacag atgaagcacc ccagggggaa
     7081 atccacggtg atgtttttca ggttgttgcc cgtggcctca ctgacacgga gcacgtgtcc
     7141 gttgccggta cgccttgtcc cgggaacggg tatgctccgg atacccttga ggtattgcca
     7201 ggtaaggctg tcctggtgcg gttcttccag gaatttgttc atgggccccg agtacagcag
     7261 gttgcctccc tcacggccgg cccccggtcc caggtctacc agccagtcgg cactcaggat
     7321 ggtttcctgg tcgtgttcca ccaccaggac cgaattcccc gcatcgcgca gtttcaggag
     7381 cgcattgata agccggcggt tgtccctctg atgcaatcca atgctgggtt cgtccaggat
     7441 gtatagcaca ttcaccatgg tggagcctat ctgtgtggcc aatcgtatac gctggctctc
     7501 tcctccgctc agggtgcgtg ttcccctgtc cagggacagg taggagagtc ccacatccag
     7561 caggaattgc aggcggtcgc ggatttcttt caggatgtct gcggcaatgg cctgttgccg
     7621 ttccgggatg cggtcttcca ctccctccag ccaggtgacc aggggctgga tatccatggc
     7681 agccagctct gcaatgttct tacctgcaaa gcgaaagcac agggcatcct ggttcagccg
     7741 gcttccgtgg cacacaggac acatcacctc ttccaggtaa cgttcccggg tggccatttt
     7801 ctcttcttcc tcaccgttca cctgttccat gcggttgatc agccccggga acgttatggt
     7861 atagggcccg cccatctctg aatgaacata gatcaggtcc tcgcttccgt agaggagtgt
     7921 atccatagct tcctcaggaa tttccgcaat gggggtatcg agtgagaatc cgtatttttt
     7981 tgccacgcct tccaggtgag cgaacaaggt gttgttcctc aatttgccaa aaggctccat
     8041 cccgccctgt gctatggaca gggaattatc cggaatgatc tttttcaggt caatacaggt
     8101 ggtgaatccc aggcctttgc attttgggca ggctcccagg ggagagttga atgaaaagct
     8161 gaagggggca ggttccggaa aagacaaccc gctttcgggg cacatcaggt gtttgctgta
     8221 gtgtgtgatg ttcccggtat ccggatccag tacagcaaag gagccgttcc cttggtgcaa
     8281 cgccatgttg acagaatcac gcaggcgggc cagtgaagca ttatcctggc cggaggggat
     8341 aagcttgtca atcacgatct caatgaagtg ggtcttgtat cggtccagca tcatgttatg
     8401 gcctatctgc tcaatgcttc cgtctatgcg gacttccaga aatccctttt tcaggatctg
     8461 gtccagcagt tcccggtaat gccccttcct tccctttacc acgggagcca ggaggatgca
     8521 tttcctgccc tggtactgcg tggcaatgag cgacaccatc tgctctgcgc tgtaccggat
     8581 catgggcttg cccgtgacgg gtgaataagc gatggacgcc ctggcaaaaa gaagcctcag
     8641 gaagtcatag atttccgtca cagtgcctac cgtggaacgg ggattgcggg tagtggtctt
     8701 ctgttcaatg gcaatgattg ggctcaggcc cgagatgtgt tccacatcgg ggcgctgaag
     8761 gatacccagg tagtggcggg cataggaaga caaggtttcc aggtagcgtc tttgtccttc
     8821 cgcatgcagg gtgtcaaagg ccagcgagga tttgccgctt ccgctcagtc ccgtaatgat
     8881 gcacaaggta tggccaggta tatccaggct gatgtttttc aggttgttga ccctggcacc
     8941 ttctatccgg atatgactgg caggcatgtg aatcagcata tatgctgcag gtaggggtta
     9001 tctgcaaatt cgcgttccag ggaagaggca ggaccgtgcc ccgggaaaac cagtgtgaaa
     9061 cgaggtaaag gtctgatttt attcacgaac gaatccatga gttgatcgaa atctcccccg
     9121 ggcaggtcgg tcctgcccac gctgccggca aagagcaggt ctcccgtgaa caaaatccct
     9181 tccaggttgc cataaaggca gacgctcccg gggctgtgac ccggggtgtg caggactttc
     9241 agggaagatg tcccgaagga aagcacctgg ccatcggcaa ggggggtgaa atccgtgccg
     9301 tgcggcgtca tgttgaatcc gaacaacaac gcctgtgaga ttgatctttt gaggatttct
     9361 gcttccaggg gatgtgccag gaacggaaca tcatatgttt tctgcaggaa accgagcccc
     9421 atcaggtgat cgaagtggca atgggtattg acaatgtacc ggggttgcag gttctggctt
     9481 ttgataaaag ctgtcagccg ctgttcttcc ctttgtctat aacagccggg atcaataatg
     9541 atgcaattgc ctgtctggtc atatgccacg tagcaacagg tgtgcaaggc attgaaataa
     9601 aaagttttaa tctccatgtt ttattttttg tgtgccggtc agcatgggca cgaatgaata
     9661 ggtaccaaaa tcttctgtgc tatatgtgtc ttctccggtt cttgtgatgc gtagcatttt
     9721 ttgttcgtaa cgtcccacgg gtatcaccag tcttccattg atggacagtt gctccttcag
     9781 cttttcggga acggaagggg ctgcgcaggt gaccaggatc cctttgaacg gggcaaactg
     9841 gggcaggccg gcaaagccgt ctccgtagta cagccctgaa ggatcaaagc ccgttgccac
     9901 caggtttttc tgtgcctgca ggtaaagctc ccatatccgt tcaatggaat acacccggta
     9961 tcccatggcc atgaggatgg cggcctggta tccgcagccg gtaccgattt ccaggacctt
    10021 gtccattttg ctgccattga gcaggtgcgt ctgcatggca acagtataag gttgcgatat
    10081 ggtctggctg ccggttatcg gcaggggctt gtcgttgtag gccatgtgct cgagcccttt
    10141 ttctacgaaa aggtgccgtg gaacctgttc catggctttc agcaccaggg gattgaaagc
    10201 aaatttacgg gccagttctt ctacaaggag ccttctttgt cctttttgcc ttagggtgtc
    10261 tgacatgtat aaaacttcaa gggaaaatga ttaagccccc tctcttccgg gaggaggctt
    10321 tcatccggtt taggaaaatg gatctatttg tccaacaatc ttaatgaagg atcaatctgg
    10381 taagcttttg ccttgtattc tgcggccttt gcattttcat taaggcgtcc gtaggcaaca
    10441 cccgttgaat acagaacgta ggggaacccc tgatccagat ccttcaacac gttcagggcc
    10501 tctttgacct tgttcttgtt caacagggca atggcgtagt tgatgtcttt gctgtcttcc
    10561 aacaggcgta aggctttatc attatcttgt tgatagatgg caagaacacc acggtagtaa
    10621 cgtgtcattc cttcatcgga gatggagttc agggcagatt cagcctggct gtactcacct
    10681 gctttcatgg ctacgcaggc acactgcaat gcgacaatgt ctttttctgc cggttcatcg
    10741 gcaaccagtt ttgcattttc cagggtctgc cttgcgcctt ccaggtcgtt gttccagaac
    10801 tggtagtatg ccaggttcac gtacagttca accacatcgg ggtagcggtc cagggacctc
    10861 ttgtaaaggg caatggcctc atcgggttcc agatccgtgg cggcctggat gatgtcgttg
    10921 ggggtcaggt actcgggctg gttatcggca attacgcgga tctcctctgc tgtcagctgg
    10981 atggggtcaa agttgactgc aaccgatccg taacgcagca ggggcatcat cacgttcttt
    11041 acctcggcgt tggaagcgat ctcggccctt atctggcgct cggcttcaac agggttggag
    11101 gccaggcgga ggaatttctc ggcaagctgg tctttgttgc ttacgttgga ttcct
//
LOCUS       DAOSOF010000056        11005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5923_length_11005_cov_5.885753, whole genome shotgun sequence.
ACCESSION   DAOSOF010000056 DAOSOF010000000
VERSION     DAOSOF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_11005_cov_5.885753"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLM68_04545"
     CDS             complement(<1..931)
                     /locus_tag="PLM68_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOT54665.1"
                     /translation="MKHLKYIIIPVLLSLFTNLSPALSQTPRTSRSGLEMQLSAIEGV
                     TEVFGLESTEFEEKYGIRMRQMVNHDDIIYGFFEQRVFIMHVGYDRPTVLVTEGYGAD
                     YASRPGYREELSRLLNANLIVVEHRYFGESVPSPRRWEYLNVRAAMTDLHKIRESLAA
                     LYPGKWIATGISKGGQTALMYRTLYPDDVDVTVCYVAPLCQGVEDGRHEPFIARKAGT
                     PESRKKVLDFQKEVLVRRDSLMPLFEKFCDTTGLHFTRPVNEIFDYCVLEYSFAFWQW
                     GTDPETIPDLTAQDTVLFAHLLQVASPDYFVRDS"
     gene            complement(943..2319)
                     /locus_tag="PLM68_04550"
     CDS             complement(943..2319)
                     /locus_tag="PLM68_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT54666.1"
                     /translation="MTRKLFLLTTLLLLFVSCERQREPDKEEAPLITQQVNKYMLELM
                     EEVYLWEKQIPDTLDVRYEFDEFEFFEKLCYRTEDRWSFITDDVQSLLEGGEGVETTF
                     GYSLAFGTFSNTGNYFAMVQYVYPQTPAAEAGIQRGTIFVEAGGKAITKDNYTTLFYA
                     PALELTLGELKDGTVYKAGTVSLTARKQSLNPVIAHTTITDGANKVGYICYTDFFGGS
                     EDLFFPVFQKFKDEGVTDIVLDLRYNLGGYLSTARALAGVLAPAGCLDGKTVLITKTW
                     NDLYQKYWKDNNRNDMLYEYFPGFSETRLNLNLSRVFILTGKNTASASELTICGLDPY
                     MNVIMIGGVTRGKYTAAMVFHPEENTRISNWGTQPIVYKYANSKGITDFKNGFTPTYR
                     EEDVLLGNVYPLGDPREALLARALSLAGMSAQVAPATQTKIQIPNVTFEDSFLSRRPG
                     LSHHLIDK"
     gene            2391..3686
                     /gene="tyrS"
                     /locus_tag="PLM68_04555"
     CDS             2391..3686
                     /gene="tyrS"
                     /locus_tag="PLM68_04555"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT54667.1"
                     /translation="MKKNNFVEELTWRGMIHDIMPGTEELLNKEMTTAYVGIDPTADS
                     LHIGHLVSVMMLKHFQVAGHRPIALIGGATGMIGDPSMKSSERNLLDEKTLRHNQECI
                     RKQLIRFLDFDDAKPNGALLLNNYDWTKDYSFLHFVRDIGKHVTVNYMMSKESVRKRL
                     SGEETDGMSFTEFSYQLLQAYDFLYLYSNYGCKLQMGGSDQWGNITTGTELIRRKQGG
                     EGYALTCPLITKSDGGKFGKTESGNVWLDPGKTTPYAFYQFWMNVSDEDAGKYIKIFT
                     LLPRETIEKHMEAHRLEPHKRLLQSVLAEYVTKMVHGEEEYNKSVTASRILFGGGTRE
                     QLMVMDEGTFLSVFDGVPQFELPASELPLTVVDLLAVRTSVMPSKGECRKLIQGGGLS
                     LNKEKVVSAEMMVDRSMLLQGKYLLVQKGKKNYFIIKAS"
     gene            3689..4045
                     /gene="rbfA"
                     /locus_tag="PLM68_04560"
     CDS             3689..4045
                     /gene="rbfA"
                     /locus_tag="PLM68_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT54668.1"
                     /translation="MKAPEAVVNTRQLKVSRQIQKDLSEIFQSKSLGTFNGTIISVVH
                     VRISSDLSVAHAWISVFPSDKATEVLKQVQEQTRTIRGELGRRVRRQLRIVPELVFFL
                     DDSLDYAEHIEELLKK"
     gene            4059..5303
                     /locus_tag="PLM68_04565"
     CDS             4059..5303
                     /locus_tag="PLM68_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT54669.1"
                     /translation="MKLAFFIAWRYLFAKKSRQIINVISAISATGITLGTMALVVVMS
                     VYNGFDNLVKGMYHTFDPDLLIKSAEGKTISGLQDFGELREDGRVAAFCPVIEETVFL
                     QYRNQQAVATMKGVDSVFIATSPLREHIISGTFHVYFGEVEEAVVGRGLANNLGMNVA
                     FVDPLWIYYPRKDAVFSPLNPMASLKREKLFPGGIFAVEQRYDADHILVSLETAQRLL
                     DYQDRFSHVEIRVNGKKALKSLKKDLETKLGPSYTVQTRYQQNETLYKMMTTEKVIIF
                     LLLGFILVIISSNVLSSLSMLILEKKEDIAVLASMGARPGLISRIFVMEGWLISILGW
                     AAGMTLGLLLCFIQTRFGVISLPGSFIVTDYPVDVQWTDMLLVSLTVLGIGFLAAYAP
                     VRSVFNKAISLAYPGNSLGVSR"
     gene            complement(5225..6085)
                     /locus_tag="PLM68_04570"
     CDS             complement(5225..6085)
                     /locus_tag="PLM68_04570"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOT54670.1"
                     /translation="MTTTSKTSDFRIGCHLSASEGYLSMAKTAVQIGANTFQFFTRNP
                     RGGTAKPIDPEDVSAFLEYSEQNDIGPILAHASYTLNLCAAEDRIREFARYTLMDDLF
                     RMEFTPGNLYNLHPGSHVQQGVEKGIELIAEALNAAITEEQTTWVLLETMAGKGTEIG
                     RTFEELRSIIDRVHVKDRLGVCLDTCHVFDAGYDIKGNTDQVLETFDRVIGLDRLKAI
                     HINDTKNPFQSHKDRHETIGNGYLGIETLRKVINHPALRHLPFYLETPNELPGYASEI
                     ALLKTERTGA"
     gene            complement(6082..8505)
                     /locus_tag="PLM68_04575"
     CDS             complement(6082..8505)
                     /locus_tag="PLM68_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT54671.1"
                     /translation="MKTKHLLPWLTLLLTGCHSDFPDVVQGFFLDYDKPAGICEERLP
                     LGNGRLGAMPDGGIATENIILNEISLWSGSPADDLRTGAHRILPVVRELLYKERIAQA
                     QELMFRYFTARTSGSEGSSGTKKTFGSYQVLGSAVLDFSYDYPPTRAEDYDRMLDIST
                     ATAFTSFELGGITYTRECFTSLDNDILVMHLTASQPNELTFRSTFTRPERYRVYTSGN
                     SLFMEGTLSSGAEDVEGMRYYTRMDIIPSGGGKLSFANDSIILSGATRATLIISAATN
                     YHYQAMDLKRDSSYIRRADSLAMLARGLSYPELKRNHVDRYQSLFRRAHVQLPDSSDF
                     YFQYGRYLLISSTRPGSLPPNLQGLWTPAVQTPWNGAYDLNMHLQMNFWPALKTNLAE
                     LHIPLLDLTARLAEMGSETAREYYNAPGWVVHSRTNVWGYTAPGVQTAWSAYNAAGAW
                     LCAHIWNHYCYTLDEDLLEKLYPVMKEAALFYLSTLMEEPRQGWWVPAPSMSYGNFFY
                     MVDEKKQGTLSVPLYLCMGSASDVQTIRELFSNVVSAHRILMNTDDFPLVYQLEEALD
                     RLPSDRISATGNLQRWLYDYPEEDTRLRNMPHLYALFPGTDITPEDTLLLEACRNTLE
                     RRGYGGSAWQMAWNMNLRARLGDGEGAFRILGALTSPALEKSVSPYLDRSVAHVTLLR
                     PGRYPNGFSSNPPFQIDGNLGACSGIAEMLLQSHRGFIHLLPAIPEAWKEAGSFSGLC
                     VQGGAMVSCSWEEGQVREIIIRAIADNTFRLKVPKNFRRIKEPYIDIPLKKGEQIRLH
                     ATSNFKPII"
     gene            complement(8583..10211)
                     /gene="groL"
                     /locus_tag="PLM68_04580"
     CDS             complement(8583..10211)
                     /gene="groL"
                     /locus_tag="PLM68_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694639.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT54672.1"
                     /translation="MAKEIKYNIEARSLMKEGVDALADAVKVTLGPKGRNVVIDKKYG
                     APQITKDGVTVAKEIELEDPFENMGAQIVKEVASKTNDQAGDGTTTATVLAQSIINVG
                     LKNVTAGANPMNLKRGIDKAVETVVSSLKKQSQSVGDDLSKIEQVAAVSANNDHEIGR
                     LIAEAMSKVKKEGVVTVEEAKGTDTEVKVVEGMQFDRGYISAYFITNSEKMEAVLENP
                     LILITDKKISTMKDLMPLLEPVAQNGRSLLIIAEDIDGEALATLVVNRLRGTLKIAAV
                     KAPGFGDRRKEMLEDIAILTGGTVITEDKGIRLESTTMDMLGAAEKVVIDKENTTIVN
                     GGGEKKAIDGRVAQIRKQMDITTSDYDKEKLQERLAKLSGGVAVLYVGAASELEMKSK
                     KDLVEDALNATRAAIEEGFIPGGGVAYIRAIEDLKKLKAENDDEATGIAIVARAIEEP
                     LRMIVENAGREGSIVAQKVREGKGDFGYNAWTDTYENLFTSGVIDPTKVARIALENAG
                     SVAGMFLTTECVLAEKKEENPPMPAGNPGMGGMM"
     gene            complement(10238..10507)
                     /locus_tag="PLM68_04585"
     CDS             complement(10238..10507)
                     /locus_tag="PLM68_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT54673.1"
                     /translation="MKIKPLADRVVVQPQEAETKTASGLYIPDTAKEKPQRGKIVAAG
                     SGKKDEPMELKEGDMVLYGKYAGTEINIDGEEYLIMRQSDVLAII"
     gene            complement(10601..>11005)
                     /gene="pnuC"
                     /locus_tag="PLM68_04590"
     CDS             complement(10601..>11005)
                     /gene="pnuC"
                     /locus_tag="PLM68_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT54674.1"
                     /translation="FMWSKRTREGSRTRVKAKNLTTKQRLILAVVCLFLTVAGALVLD
                     HFDDPAPVLDSATTFLSIVAMYLMVKTYSEQWYLWILVNLATITMWGMAFFRENQPGH
                     LVIASMWCVYLLNSIHGLAVWKRAAKSATLTN"
BASE COUNT         2644 a         2702 c         2820 g         2839 t
ORIGIN      
        1 gagaatccct gacaaagtaa tccggagaag cgacttgtaa caggtgggca aaaagaacgg
       61 tgtcctgagc tgtaaggtcc gggattgtct ccggatcagt accccattgc cagaatgcaa
      121 aggaatattc aagcacgcag tagtcaaata tctcattcac gggccgcgtg aaatgcaacc
      181 ccgttgtatc acagaatttc tcgaaaaggg gcataagact gtccctccga accaggacct
      241 ctttctggaa atcaagcact ttcttacggg attcgggagt tccggcctta cgggctatga
      301 aaggctcgtg tcttccatcc tccacgccct ggcaaagagg ggccacataa caaacagtga
      361 catctacatc gtcgggataa agggtacgat acatcaaggc ggtctgacct cctttgctga
      421 tgcctgtggc aatccatttg ccggggtaaa gcgctgcaag gctttccctg atcttgtgca
      481 ggtcagtcat ggctgccctt acgttcaggt attcccacct gcggggggac gggactgatt
      541 ctccaaaata ccggtgttcc acaacgatca ggttggcatt cagcagtcgg gacaactctt
      601 ccctgtatcc cggcctggag gcataatccg cgccatatcc ttctgtcacc aatacggttg
      661 gacgatcgta tccgacgtgc attataaaaa cacgttgttc aaaaaaccca tatatgatgt
      721 catcatggtt gaccatctgg cgcatgcgta tgccgtactt ttcttcaaat tcagtgcttt
      781 ccagtccgaa aacctcggta accccctcga tggctgaaag ctgcatttca aggcccgagc
      841 gggatgtgcg cggcgtttgt gaaagtgcgg gagaaaggtt ggtaaataaa gaaagaagga
      901 caggaatgat gatgtattta agatgtttca tgaaagatgt ggttatttgt caatcaaatg
      961 gtgggacaat ccgggacgcc tggaaaggaa ggagtcttca aaagtgacat tgggtatttg
     1021 aatttttgtt tgcgtggccg gagcaacctg tgcgctcatg ccagccagcg acaaagcacg
     1081 tgccagcagc gcttcacggg ggtctcccag gggatagaca ttgcccagca gaacgtcttc
     1141 ttcacggtaa gtgggtgtga acccattctt aaaatctgtg atgcctttgg aattggcata
     1201 cttgtacact atgggctgtg ttccccagtt gcttatacgc gtattctcct cgggatgaaa
     1261 taccatggca gccgtgtatt ttcctcttgt aacacctcca atcatgatca cattcatata
     1321 gggatcaagc ccgcagatgg tcagctccga ggcgctggca gtattctttc ctgtcaggat
     1381 gaaaacccgc gacaggttca ggttgaggcg tgtttcggaa aacccgggaa agtattcgta
     1441 aagcatgtca ttccggttgt tgtctttcca gtatttctgg tacaggtcgt tccaggtttt
     1501 ggtgatcagc accgttttac catccagaca cccggcagga gccagcacac cggccagcgc
     1561 ccgggccgtg ctcaggtatc cgcccagatt gtacctaagg tccaggacaa tgtcggttac
     1621 tccttcatct ttgaatttct ggaataccgg gaagaacaaa tcttctgaac ccccgaagaa
     1681 atcggtataa catatgtacc ccactttatt ggcaccatca gtaatggtgg tatgggctat
     1741 caccggattc aggctttgct tgcgggcggt cagagaaact gtgccggcct tgtagacggt
     1801 cccatccttg agctccccca aggtcagctc cagcgcgggt gcatagaaga gcgtggtata
     1861 attgtctttg gtgatggctt ttccgccggc ttcaacaaaa atggtaccac gttgaatccc
     1921 ggcttctgct gcaggtgtct gaggatatac atactggacc atggcaaaat aattgccggt
     1981 attggagaaa gtgccaaagg ccagggaata gccgaaagtt gtttccaccc cttctcctcc
     2041 ttccagcagg ctctggacat catcagttat gaaagaccag cggtcctcgg tgcggtaaca
     2101 caacttctca aaaaattcga actcatcgaa ttcataacgt acatccagcg tgtcgggtat
     2161 ttgcttttcc cagaggtata cttcttccat caattcaagc atatacttgt ttacctgctg
     2221 tgttatcaac ggggcttctt ccttatccgg ttccctctgc ctttcgcagg acacgaatag
     2281 caaaagaagg gttgtaagca aaaagagttt ccttgtcata aaagtagaat atcccgctaa
     2341 agtacaaatt tttattcttt acctttgcac aaaacatacc aagacagagg atgaagaaga
     2401 ataattttgt agaagaactt acctggaggg gtatgatcca cgacattatg ccgggtaccg
     2461 aagaattgct taacaaggaa atgaccactg cctatgtagg tattgatccc acagccgatt
     2521 cactgcacat aggtcacctg gtaagtgtaa tgatgctgaa acattttcag gttgccggac
     2581 accgtcccat agccctgata ggcggagcga ccggaatgat aggggatcca tccatgaaat
     2641 cttcagaaag aaaccttctg gatgaaaaga cgttgaggca caaccaggaa tgcatcagaa
     2701 aacaattgat acgctttctt gattttgatg atgccaagcc taacggagcc ctgttactga
     2761 acaattatga ctggacaaag gattattctt tcctccattt tgtccgtgac attggcaagc
     2821 atgttacggt caattatatg atgagcaagg aatctgtacg caaaaggctt tcgggggaag
     2881 aaacagatgg gatgtccttt accgaattca gctaccagct tttacaggct tacgattttc
     2941 tttatttgta cagcaattat ggatgtaaat tgcagatggg cggatctgat cagtggggca
     3001 acatcaccac cggtacggag ctcatacgga gaaaacaggg cggagaaggc tatgcgctga
     3061 cttgcccgct gatcaccaaa tcggacgggg gcaagtttgg aaaaacggaa tcgggcaacg
     3121 tgtggcttga ccccgggaaa accactccct acgcgtttta tcagttctgg atgaacgtca
     3181 gcgacgaaga tgcagggaag tacatcaaga tattcaccct gctgcccagg gaaaccatag
     3241 aaaaacacat ggaagcccac aggttggaac cgcataaaag gctactgcaa agcgttttgg
     3301 ccgaatatgt aacaaagatg gtacacggag aagaagaata taacaaatct gttacggcgt
     3361 cccggatttt gtttggggga ggcacaaggg aacaacttat ggtcatggac gagggtactt
     3421 tcctgtctgt ttttgacgga gtaccgcaat ttgaattgcc cgccagtgaa cttcccctga
     3481 ctgtagttga tttgctggca gtgcggacat ctgtcatgcc gtccaagggg gaatgccgta
     3541 aattgatcca gggcggaggg ctgagcctta ataaagaaaa ggttgtgtct gctgaaatga
     3601 tggtagacag atccatgctc ctccagggca aatacctgct ggtacagaag ggaaaaaaga
     3661 attatttcat cataaaagcc agttgatcat gaaagcaccg gaagcagttg tgaacacccg
     3721 gcaattgaag gtgtcacgtc agatacagaa agatctttcg gagatcttcc agtcaaagtc
     3781 cttgggtacg tttaacggaa ccataatctc tgtggtgcac gtccggatca gctccgacct
     3841 gtccgtggcg cacgcgtgga taagtgtgtt tccatccgat aaggccacgg aagttttaaa
     3901 gcaggtacag gaacagaccc ggaccattcg cggagagctg ggacgtcgag ttcgcagaca
     3961 attgcgaatt gtaccggaac ttgtcttttt cctggatgat tcactagatt atgcagagca
     4021 catagaggaa ttgctgaaga aataaacaag ccataatttt gaaacttgcc tttttcattg
     4081 cctggcggta tttgtttgcc aagaaatcaa ggcagatcat caatgtgata tctgccatta
     4141 gtgccacggg gataaccctg gggaccatgg ctttggtggt ggtcatgtct gtctacaacg
     4201 gctttgacaa cctggtaaag ggtatgtacc atacctttga tccggacctt ctcattaaaa
     4261 gcgcggaagg aaaaaccatt tccggactgc aagattttgg tgagctgagg gaggacgggc
     4321 gtgtggcggc tttctgcccg gtcattgaag aaacggtgtt tctgcagtac aggaaccagc
     4381 aagcagtggc caccatgaaa ggagtggaca gtgttttcat agccacttct ccattgaggg
     4441 agcatatcat tagcggcaca ttccatgtgt attttggaga ggtggaagag gccgtggtgg
     4501 gaagaggcct ggcaaataac ctgggcatga acgttgcttt tgtggatcct ttatggatct
     4561 actatccccg aaaggatgcc gttttttccc cgctcaatcc catggcctcc ctcaaacggg
     4621 aaaaactctt tccgggagga atttttgctg tcgaacagcg atacgatgcc gaccatatcc
     4681 tggtctccct ggaaacggcc caaaggcttc tggattacca ggacaggttc tcacatgtgg
     4741 agatacgcgt gaacgggaaa aaggccctca aatcgcttaa aaaagacctg gaaacaaagc
     4801 tcggcccttc ttataccgtg cagaccaggt accagcaaaa cgaaaccctt tataaaatga
     4861 tgacgacaga aaaggtcatc atattcttat tgctgggttt catcctggtg atcatttcta
     4921 gcaacgttct cagctccctg agtatgctca tcctggaaaa aaaggaagac attgcggtcc
     4981 ttgccagcat gggagcgcgt cccggtctca taagcaggat atttgtcatg gaaggatggc
     5041 tcatttcaat cctgggctgg gctgccggta tgacactggg tttgttgctg tgctttatcc
     5101 agacgcgatt cggcgtgatt tccctcccgg gaagctttat tgtaacggat tatcctgtag
     5161 atgtccagtg gacagacatg ctcctggttt cgttgaccgt cctgggaata ggattcctgg
     5221 cggcttacgc tccggtgcgc tccgttttca acaaggctat ttcgctggca taccccggta
     5281 actcattggg tgtttccagg taaaaaggaa gatgccgcag ggccggatga tttatcacct
     5341 tacggagcgt ttcaatgccc aggtatccgt tgcctatggt ttcgtgcctg tccttatggc
     5401 tttggaaagg attcttggtg tcgttgatat ggattgcctt caagcgatcc aggccgatta
     5461 ccctgtcaaa tgtttccagc acctggtccg tattgccctt gatgtcataa ccggcatcaa
     5521 acacatgaca cgtatccagg cagacgccca gcctgtcctt cacatgaaca cggtcgatta
     5581 tggacctgag ttcctcgaaa gtcctgccga tttcggtccc ttttccggcc atggtttcca
     5641 gcaggaccca ggtggtctgc tcttcggtga tggctgcatt cagggcttct gctatcagct
     5701 ctatgccttt ttccacccct tgctgcacgt gacttcccgg atgcaggtta tacaggttcc
     5761 ccggtgtgaa ctccatcctg aaaaggtcat ccatgagggt gtagcgtgcg aattcccgga
     5821 tacggtcttc ggcagcgcac aggttcaggg tataggaggc atgtgccagg atgggaccta
     5881 tgtcgttttg ttctgaatat tccaggaagg cagatacatc ttccggatct atgggttttg
     5941 cagtacctcc gcggggattg cgtgtgaaga actgaaaagt gttggctccg atctgaacgg
     6001 ctgtcttggc catggagagg tagccttctg atgcggaaag gtggcatccg atgcggaaat
     6061 cgctggtctt tgaagtggtc gtcatattat aggtttaaaa ttacttgtgg catgcagtct
     6121 tatctgttct ccctttttca agggaatatc tatatacggt tcttttatgc ggcggaaatt
     6181 ttttggtact ttcagccgga aggtgttgtc agcaattgcc ctgataataa tttcacgcac
     6241 ctgtccttct tcccaggagc aggacaccat ggcgcctccc tgtacacaga gaccagagaa
     6301 tgaccccgcc tctttccatg cttcgggaat ggccggcagt aagtgtatga acccccggtg
     6361 gctctggagc aacatttctg caatgcccga acatgcccca agattaccgt cgatctggaa
     6421 agggggattg gatgaaaaac cattgggata tcttcccgga cgaagcaggg taacatgtgc
     6481 aacagagcgg tccaggtaag gggaaacact tttttccagg gcgggggaag tcagggcacc
     6541 caggatcctg aaggcaccct caccatcgcc gagtctggcc ctcaggttca tgttccacgc
     6601 catttgccat gccgaaccgc cgtaacctct tctttccagc gtgttgcgac aggcttccag
     6661 cagcagggtg tcttccgggg taatgtctgt accgggaaac agtgcataaa ggtgtggcat
     6721 attgcgcaga cgtgtgtctt cttcgggata atcatagagc catcgctgca ggtttccggt
     6781 agcactgatc ctatccgaag gtaaccggtc cagggcttcc tccagttggt agaccagggg
     6841 aaaatcgtcc gtgttcatca ggatcctgtg ggcagaaacc acatttgaaa ataattcccg
     6901 tattgtctgg acatcacttg cagagcccat gcaaaggtat agcggaacgg acaatgtgcc
     6961 ctgtttcttt tcatccacca tatagaaaaa gtttccgtaa gacatggaag gagcgggaac
     7021 ccaccacccc tggcggggct cttccatgag tgtggataaa tagaaaaggg cggcttcttt
     7081 cattaccggg tacagctttt ccagcaggtc ttcatccaat gtatagcagt aatggttcca
     7141 tatatgggca catagccagg caccggccgc gttataagcg gaccatgccg tttggacacc
     7201 gggagctgta tatccccaga cgttggtcct ggagtgcacc acccatcccg gggcgttgta
     7261 atattccctg gctgtttcgc ttcccatttc agccaaccgg gcggtaagat caaggagggg
     7321 aatatgcagc tcggcaaggt tggttttcaa ggcaggccag aaattcatct ggaggtgcat
     7381 attcagatca taggcgccgt tccagggtgt ctggacagca ggggtccaca aaccctgcaa
     7441 attgggtgga agtgatcccg ggcgtgtgga agagatgagg aggtacctcc cgtactgaaa
     7501 gtaaaagtcc gaggaatcgg gcaattgtac atgagccctg cgaaaaagag actggtacct
     7561 gtccacatgg ttgcgtttca attcaggata cgacaacccg cgagccagca tggccaggga
     7621 atctgcccgt ctgatgtacg aagagtccct tttcaggtcc atggcctggt agtggtaatt
     7681 tgtggctgca gagatgatca gggtcgcccg ggtggcaccg gacaggatga tggagtcgtt
     7741 ggcaaaggat aacttaccgc ctcccgacgg aatgatatcc atgcgtgtgt agtagcgcat
     7801 gccctccaca tcctctgccc ccgagctcaa tgtgccttcc atgaacaggg aattgccgct
     7861 ggtatagacc cggtatcttt ccggacgcgt gaaggtggac cggaaggtca gttcgttcgg
     7921 ctgggatgcg gtaaggtgca tgaccagtat atcgttgtcc agcgaagtga agcattccct
     7981 ggtatatgtt atgcctccca gttcaaagga cgtgaatgcg gttgcggtgg atatatccag
     8041 catcctgtca tagtcctcgg cccgggtcgg ggggtagtca taagaaaaat ccagtaccgc
     8101 ggaacccagg acctggtaac tcccaaaggt ttttttggtt ccgctgctcc cttccgaacc
     8161 ggatgtgcgt gcggtgaaat aacggaacat aagctcctgt gcttgggcga tacgttcttt
     8221 gtaaagcagt tcccgtacca cgggcagtat gcggtgtgcg cctgtccgca ggtcatcagc
     8281 aggggatccc gaccagagag agatctcgtt aagaataata ttttcagtgg cgatacctcc
     8341 gtcaggcatg gcaccaagcc tgccgttacc caggggtaac ctttcttcgc agatacctgc
     8401 aggcttgtca taatccagga aaaaaccctg tacaacgtca ggaaagtctg aatggcaacc
     8461 tgtcagtaaa agcgtaagcc atggcagcag gtgctttgtc ttcataaaac gaagatacac
     8521 aaaaaaaaag aacacggaag acgtttgccc tccgtgttcc ccgtgttata attagctgaa
     8581 tattacatca ttccgcccat tccgggattc cctgccggca tggggggatt ctcttctttc
     8641 ttttctgcca gaacacattc tgtggtcagg aacatccctg cgacagagcc tgcattctcc
     8701 agggcaatcc tggccacctt ggtaggatcg atcacacccg atgtgaacag gttttcgtat
     8761 gtatcggtcc aggcattgta tccgaagtca cccttgcctt cacgtacttt ttgagccaca
     8821 atgctgcctt ccctgccggc attctccacg atcatgcgca gaggctcttc aatggcgcgg
     8881 gccacaatgg cgataccggt agcttcgtcg tcgttctcgg ctttcagctt tttcaggtct
     8941 tcgatggcac gtatgtaggc cacaccgcct ccggggataa acccttcctc gatggctgca
     9001 cgggtggcgt tcagcgcgtc ttctaccagg tctttcttgg atttcatctc caattcggaa
     9061 gcagctccca cgtacaaaac ggcaaccccg ccggacaatt tggccaggcg ttcctgcaat
     9121 ttttccttat cgtaatcact tgtggtgatg tccatctgtt tacggatctg tgccacgcgt
     9181 ccgtcaatgg ccttcttttc gcctccaccg ttcacgatgg tggtgttttc cttatctatg
     9241 acgacttttt ctgcagcccc gagcatgtcc atggtggtgg attcaagccg gatgcccttg
     9301 tcttccgtaa tgacagtgcc acctgtcagg atggcgatgt cttccagcat ttccttgcgg
     9361 cggtcaccaa atccgggagc tttgacagcg gcgatcttca gggttccgcg caggcggttg
     9421 accaccaggg ttgccaatgc ctcaccatca atgtcctcgg caatgatcag cagggaacgg
     9481 ccgttctggg caacaggctc cagtagcggc ataaggtctt tcatggtcga gattttcttg
     9541 tcggtgatca ggatgagggg attttccagg acagcttcca ttttttccga attggtaatg
     9601 aaataagccg agatgtagcc ccggtcaaac tgcattcctt ccacaacctt tacctctgta
     9661 tcggttcctt tggcttcctc cacggtaaca acaccttctt tcttgacctt tgacatggct
     9721 tctgctatca gcctgccaat ttcatggtca ttgttggccg agacagcggc aacctgttca
     9781 atcttggaca ggtcatcgcc caccgactgg ctttgcttct tcaaactgga aacaacggtt
     9841 tccacggcct tgtctatacc acgtttcagg ttcatgggat ttgcaccggc tgtcacgttc
     9901 tttagtccca cattgatgat ggactgggcc agcacggtag cggtggtggt cccgtcacca
     9961 gcctggtcat tggtcttgga agcgacttcc ttgacaatct gagcgcccat gttttcaaac
    10021 ggatcttcca gttcgatttc cttggctacc gtcacaccgt ccttggtgat ctggggagca
    10081 ccgtatttct tatctatcac cacattgcgt ccttttggac ccaaagtcac tttcacggca
    10141 tctgccagag catctacccc ttctttcatc agggagcggg cttcaatatt gtatttgatt
    10201 tcttttgcca tagttgttgg tttttatttg attaatgtta aataatggct aaaacatctg
    10261 actgacgcat gatgagatac tcttctccgt caatgttgat ctctgttccg gcatacttgc
    10321 cgtaaagcac catgtcacct tctttcaatt ccataggctc atcttttttt ccgcttccgg
    10381 ctgccacgat ttttcctctt tgtggttttt cttttgccgt gtcgggaatg taaagtcccg
    10441 aggctgtctt ggtctcagct tcctgcggct gaacgactac gcgatctgct aaaggtttga
    10501 ttttcatttt tgatacattt taaagttaat tattgaattt gtgatttttc agtcaacgaa
    10561 tggagaaaag gcaaaatcaa tgccagagaa atgatgtctg tcaatttgtc agcgtggccg
    10621 atttggcagc ccttttccaa accgccagcc cgtgaatgga attaagaagg taaacgcacc
    10681 acatagaggc aatgaccaga tgtcccggct ggttctcccg gaaaaaggcc atgccccaca
    10741 tggttattgt tgccaggttt accaggatcc ataagtacca ctgctcggaa tatgttttca
    10801 ccataagata catggccact atggaaagga aggttgtagc ggagtcaaga accggcgcgg
    10861 ggtcatcaaa atggtcaagc accagggcac cggccaccgt aagaaacaga cagaccacgg
    10921 ccagtatgag gcgttgtttt gtggtgaggt tttttgcctt gacacgggtg cggctgcctt
    10981 cccgggtgcg tttgctccac atgaa
//
LOCUS       DAOSOF010000057        10922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_5991_length_10922_cov_4.794884, whole genome shotgun sequence.
ACCESSION   DAOSOF010000057 DAOSOF010000000
VERSION     DAOSOF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5991_length_10922_cov_4.794884"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLM68_04595"
     CDS             complement(<1..919)
                     /locus_tag="PLM68_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT54675.1"
                     /translation="MKGSISQIIGPVVDVVFSSGTKDVQLPSIRHALEVRRPDGKKII
                     LEVQQHIGDNTVRTVAMDSTDGLSRGLEVDTTGSPITMPIGAQVKGRLLNVIGQTIDG
                     LRPLSADTGTAPIHKAPPKFQDLTTSSEVLYTGIKVIDLLEPYLKGGKIGLFGGAGVG
                     KTVIIMELINNIAKKHSGYSVFAGVGERTREGNDLLREMIQSGVIRYGDAFLESMKNG
                     AWDLSKVDYNELAKSQATLVFGQMNEPPGARASVALSGLTIAEAIRDEDGQVRDILLF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPT"
     gene            1015..2847
                     /locus_tag="PLM68_04600"
     CDS             1015..2847
                     /locus_tag="PLM68_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOT54676.1"
                     /translation="MTTPPSTYTEDNFQTLEWNQHIRRRPGMYIGKSGDGSTPDDGLY
                     VLIKEVIDNSIDEYAMGYGKVISVTIRDNVVSVRDYGRGIPFGKVIEAANKMNTGAKY
                     DNKSFQKAVGLNGVGLKAVNATSIRFYIESFREGRSRFGIFSQGVLLEEGERESGQKD
                     GTLVEFKPDPALFNHYSINLDFVWEMVRHYAYLNKGLRMQLNGTEFISKNGLLDLVNE
                     SMAEETLYPPIHLAGTDIEMVITHTNDTGESIMSFANGQNTSQGGTHLSAFREAVART
                     IKEFYKKDFESSDIRQSIVGAISIRLIEPRFANQTKTILDSREMSNSGPTLRSFIGDF
                     VKEQLDNYLHKNPETAEILLKKIIENEKERKAVGLVRKKAKENAKKISLHNRKLRDCR
                     IHFTDKHELAGQTMLFITEGDSASGSITKSRDPNIQAVFSLRGKPENTFGLTKQVVYT
                     NEEFNLLQAALNIEDDLDNLRYNKIIIATDADPDGMHIRMLLLSFFLQFFPELVRHGH
                     IYILQTPLFRVRNKKETRYCYTETEREKAVRELGPDPEITRFKGLGEISPDEFRGFIG
                     DNIRLDIVRLDKDDRLHELLRFYMGDNTPERRDFIVNNLRMVGD"
     gene            2851..5520
                     /locus_tag="PLM68_04605"
     CDS             2851..5520
                     /locus_tag="PLM68_04605"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOT54677.1"
                     /translation="MMSEDQLKYLRILGEEQDKKYRLTGMFKDWYLEYSSYVILERAI
                     PHLTDGLKPVQRRILHSMKRMEDGRFNKVANIIGHTMQFHPHGDASIGDALVQLGQKE
                     LLIETQGNWGNVLTGDSAAAPRYIEARLSKFALDVVFNPKTTVWIPSYDGRNQEPEAL
                     PVKFPLLLAQGAEGIAVGLATKILPHNFNELIEASIAHLKGEPYTLYPDFMTGGLADC
                     SNYNGGIGNSRIKVRAKIIKADKKTLVIKEIPYGQTTDSLIRSIITANDKGKIKIRKI
                     DDNTAEQVEILIHLANDVSPDKTIDALYAFTKCEVSISPDTCVIMDGKPFVTSVENML
                     GYSTERTRDLLKRELEIRLDELEDNWHFSSLEKIFFEEKVYRILENNARTWEDQLKDV
                     ETEMLRYQDRLRRPVVFAEIEKLVEKPVRKISRFDIKKATEHIKSLDREMEQIRHNLE
                     HLTAYAIEYYEGLKKKYGSFFPRKTEITSFQTIEASNVILANCKLYVSRESGFVGADM
                     KKDDKAEYLCDCSSLDDVMVFLKDGTYSVRKVSDKQYVGKDIIYAGILRKKENRTVYN
                     AVYRDGKNGKVFAKRFQMNSFSRDRDYNLTQGTPGSQVLWLSVNPNGEAETIRVFLKS
                     RPKLKKLIFEFSFLDLAIKGRSSRGNILSRHPVKKITLKDKGIATLGGQEIWFDKDIQ
                     RLNDQGRGLYLGEFHADDSLLAINRNGTFCTTNFDLSNRYQEEVLFIEKMDPEKTFSA
                     VYYDSDAEAYYVKRFRFESSENMVQSFIGEGEGSYLVAISSDKNPVVKVTFGGKHEKR
                     PPQEINVTEFIGEKSFRAKGKRITTFQVEKIEFCESASANEDTLPDNGDDGSLDFELE
                     GPVSKGDVAPVPSADKNPLADFNDQDAIQMMLL"
     gene            5531..5989
                     /locus_tag="PLM68_04610"
     CDS             5531..5989
                     /locus_tag="PLM68_04610"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT54678.1"
                     /translation="MGLIFYIGFMASGKSTLGAADAARDGYKFTDLDQKLWDNTGIPP
                     WQYITEKGEEAFRIAERDILHWIVEEWHREGQPPQLVACGGGTPCAFDNMEFMKSNGQ
                     VIFIDTPLSTILERIRNNPHRWPLAGISNLESLYLERRKWYEKAHERKVP"
     gene            complement(5986..7719)
                     /gene="recJ"
                     /locus_tag="PLM68_04615"
     CDS             complement(5986..7719)
                     /gene="recJ"
                     /locus_tag="PLM68_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT54679.1"
                     /translation="MALEKKWIVKEPGNPALVRQLVAELGIDNALANLLVQRNVKDFA
                     QAKSFFRPQLENLHDPFLMKDMDKAVKRLDRAIRESEKILVYGDYDVDGTTAVALVYS
                     FLRKGTPMVSYYIPDRYTEGYGISYIGIDAAAEEGCTLIIALDCGIKATEKVEYARHK
                     GIDFIICDHHLPADELPQAVAVLDPKRNDCHYPFDELSGCGVGFKLLQAFCMSRNIPL
                     TELYPFLDLVVVSIASDLVSITGENRILAHYGLKQINEAPRKGLHSIIKLAGLEKHQI
                     TVDDIVFKIGPRINAAGRMESGKMAVDLLISRTDDDASQIGNTINDHNDDRKNIDRKI
                     TAEALDMVRDMPGLKDKKSTVVYNPKWHKGVVGIVASRLVENYYRPTIVLTKSNGFIT
                     GSARSIPGFDLYEAIENCSDLLENFGGHMYAAGLTMKEERYQEFCQRFEDIVAEKVTE
                     EMLTPLESIDTYIEFRQITPKFFRLLKQFQPFGPGNLSPVFVTENVYDNGNGRLVGSD
                     SGHLKLELIQEDEPYRPISAIAFNQSEHFEHMHNGNPVDICYSVAENYYRGIANIQLR
                     IKDIKTRDVIV"
     gene            7799..9307
                     /gene="lysS"
                     /locus_tag="PLM68_04620"
     CDS             7799..9307
                     /gene="lysS"
                     /locus_tag="PLM68_04620"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT54680.1"
                     /translation="MENTHISEQEQVRRDALTEIKKLGIDPFPAATYPVDTTAAEILE
                     GYDPEIENFKAVTLAGRIMSRRIMGAASFMVIQDHSGRIQVYVKRDDICPTEDKTLYN
                     TVFKKLLDIGDIVGIRGFAFITMTGELSVHARELVLLSKSLRVLPIIKEKEGQVYDAF
                     TDPEQRYRMRYVDLIVNPQVRETFVKRSKMVNIMRDFFNSQGYLEVETPILQPIPGGA
                     NARPFITHHNALDIPLYLRIANELYLKKLIVGGFPGVYEFSKDFRNEGMDRTHNPEFT
                     VLEIYVAYKDYLWMMNFTEEMVERIALELHGSTRVPYGDNVIDWKRPFRRISMTDAIK
                     EYAGVDVTDMEEEDLRRTCMDLNVHITPFMGKGKLIDALFGEFCEKHLIQPTFITDYP
                     VSTSPLTKRHREKLHLTERFELFVNGKELANAYSELNDPLDQLERFKEQLRLKEKGDD
                     EAMFIDMDFIRALEFGMPPTSGMGIGIDRLCMLMTNAPSIQDVLFFPQMRPE"
     gene            9362..10099
                     /locus_tag="PLM68_04625"
     CDS             9362..10099
                     /locus_tag="PLM68_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT54681.1"
                     /translation="MEKSRVRREKGLFVIEGIRELEIALKSGYIIQELYLCPAILQDA
                     EKASWFREHSLISDDLEAGLYGKLAYRGTTEGVLAVMRTRKMKPEEILLQEPALVLVL
                     ESVEKPGNLGAILRTADASGAGAVLICDPLTDLYNPNTIRASLGCIFSVPTLACTSDE
                     AYKWLSRQGIQVVAATCQATSWYHECDYRKPTALVLGSESDGLRPFWREKAAKEVKIP
                     MMGIIDSLNVSAAAAILCFEALRQRNA"
     gene            10102..10839
                     /locus_tag="PLM68_04630"
     CDS             10102..10839
                     /locus_tag="PLM68_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54682.1"
                     /translation="MGPFAIIGNPVTSSLSPRLFSAAYPQLPREMYLVIEAPDAEQGW
                     KRIKELHIQGFNVTMPFKTDFLNMADKLAPAVRQVRATNLLTLEDGIWTAWNTDITGV
                     VNSFRGSGIDPAGKNALIIGAGGAGRAAALGLEQAGATVFIANRTLRPRLYPLSDIPS
                     LLRKCTLIVNTVPCEGIDAAVINLTCAHTILDASYTLAPLRKAAACAGARYLGGYHWL
                     YNQAVEGFAILTGLEPDCRAMRKLLDL"
BASE COUNT         2976 a         2621 c         2728 g         2597 t
ORIGIN      
        1 gggtgggctg gtatcccact gccgaaggca tcctgcccag aagggccgag acttcagaac
       61 cggcctgggt gaaacggaat atattgtcta tgaacagcag aatatcacgc acctgtccgt
      121 cttcatcgcg aatggcctca gcaatggtca gaccgctcag ggctaccgat gccctggctc
      181 cgggaggttc gttcatctgg ccgaaaacca gggtagcctg tgatttggcc agttcattgt
      241 aatccacttt ggacaggtcc catgctccat ttttcatgct ttcaaggaaa gcatctccgt
      301 agcggatgac tccggactgg atcatttccc gcagcaaatc gttcccttcc cgggtgcgtt
      361 ctcccactcc ggcaaagacg gagtaaccgc tgtgtttctt ggctatgttg ttgatcagct
      421 ccatgatgat cacggttttg cccacccctg ctcctccaaa aagaccgatc tttcctccct
      481 tcaggtaagg ttccagcagg tctatgactt ttatgccggt atacaggacc tcactggaag
      541 tggtcaggtc ctggaacttc gggggtgcct tgtgtatggg ggcagtgccg gtatctgcac
      601 tgagtggtct gagtccatca attgtctggc ctatcacgtt caacaggcgg cccttgacct
      661 gtgcccctat gggcatggtt atggggcttc cggtagtatc cacttccaga cccctggaaa
      721 gaccatcggt agagtccatg gcaacagtgc gcactgtgtt gtccccgata tgttgctgta
      781 cttccagtat gatcttctta ccatcgggac ggcgtacttc cagtgcatgg cgtatagaag
      841 gcaattgcac gtcttttgtt ccggaggaga acacaacgtc cacaacaggg ccgattattt
      901 gtgaaatact acccttcata gcagtgttac agatttgtta gtttctttgt acaaactgca
      961 aatgtaatga tttattgaac tatttcaacc agttttttta tctttgtgcc ccgtatgacc
     1021 acccctccat caacttatac agaagataat ttccagactc ttgaatggaa tcaacacatc
     1081 cgtcgcaggc cgggtatgta cattggaaaa tcaggtgacg gaagcactcc cgacgatgga
     1141 ctatatgtcc tgatcaagga agtcattgac aactccattg atgaatatgc catggggtat
     1201 ggaaaggtga tctccgttac catacgggac aatgttgtct cggtccggga ttacggccgg
     1261 ggaatccctt tcggaaaagt gatcgaggct gccaataaaa tgaataccgg ggccaaatac
     1321 gataacaaat cctttcagaa agccgtggga ttgaacgggg tgggccttaa ggccgtaaat
     1381 gcaacttcca tcaggtttta tatagaatcc ttcagggaag ggaggagtcg tttcggaata
     1441 ttctcccagg gtgtcctgct ggaagaaggc gagagggagt ctggtcagaa agacgggaca
     1501 ttggttgaat tcaagccgga cccggccttg ttcaaccact attcgatcaa cctggatttt
     1561 gtctgggaaa tggtcaggca ttatgcctac ctgaacaaag gcctgcgcat gcagctcaac
     1621 ggcaccgaat tcatctccaa aaacggtttg ctggacctgg tcaatgaaag catggccgaa
     1681 gagaccctct atcctcccat acaccttgcc ggaacggata tcgagatggt tatcacccat
     1741 accaacgata ccggggaaag catcatgtct tttgccaacg ggcaaaacac atctcagggc
     1801 gggacacacc tttccgcttt ccgggaagcc gttgcccgga caattaaaga gttttacaaa
     1861 aaagattttg aatcctcaga tatccggcaa tccattgtag gagccatcag cattcgcctt
     1921 attgagccca gattcgccaa ccagacaaag accatcctgg actcaaggga aatgagcaat
     1981 tctggtccga cactccgaag tttcatcgga gattttgtca aagaacagct ggacaattac
     2041 ctgcataaga acccggaaac tgcggagatc ctcctgaaaa agatcattga aaacgaaaaa
     2101 gaaaggaaag ctgttgggct tgtacgcaaa aaagcaaaag aaaatgcgaa gaagataagc
     2161 ctgcacaacc ggaaattacg ggactgccgt atccatttta ccgataagca tgaactggcc
     2221 ggacaaacca tgcttttcat aaccgaaggc gattctgcaa gcggttccat tacaaaaagc
     2281 agggatccca acatacaggc tgtctttagc ctgagaggca aaccggagaa cacctttggc
     2341 cttacaaaac aggtcgtcta taccaatgag gaattcaacc tgcttcaggc tgccctgaac
     2401 atagaagacg atctggacaa cctgcgctac aacaagatca tcatagcgac cgatgccgat
     2461 ccggatggaa tgcacataag aatgttattg ctgagctttt tcctgcaatt cttcccggaa
     2521 ctggtaagac acggccatat ctatatcctg cagacacccc tgttcagggt aaggaacaaa
     2581 aaagagacac gttactgtta cacagaaacg gaaagggaaa aagcagtgcg tgagctgggc
     2641 cctgatccgg aaatcacccg gttcaaaggc ctgggtgaga tctcaccgga tgagttcagg
     2701 ggtttcatag gagataatat ccgtcttgac attgtgcgcc tggacaagga tgacaggctt
     2761 cacgagttgc tcaggttcta tatgggagac aacacgccgg aaagaaggga tttcattgtc
     2821 aacaatttaa gaatggtggg ggattaggcc atgatgagtg aagaccagtt gaagtaccta
     2881 cgtatcctgg gtgaagaaca agacaagaaa tacaggctta caggcatgtt caaggactgg
     2941 taccttgaat actcttccta tgtcatactg gaacgcgcca tcccccacct gaccgacggt
     3001 ttgaaaccgg tgcagcgaag gatactccat tccatgaaaa ggatggaaga cggccgtttc
     3061 aacaaagtcg ccaacatcat cggacatacc atgcaattcc atccgcatgg ggacgcttcc
     3121 attggagatg ccctggtgca gctgggacag aaggagttgc tcatagaaac tcaggggaac
     3181 tggggaaatg tgctcaccgg ggattccgcc gcggcacccc gttatataga ggcacgcctg
     3241 agcaaatttg cactggatgt agttttcaat cccaaaacca cagtatggat accctcttac
     3301 gacggcagaa accaggaacc ggaagccctt ccggtcaaat ttccattatt gctggcacag
     3361 ggtgcagaag ggattgcagt cgggcttgcc accaagatcc tcccccataa tttcaatgaa
     3421 ttgattgaag cttccattgc ccatctcaag ggggaaccct atactctgta tcccgatttc
     3481 atgacaggcg gtcttgccga ctgcagcaat tacaacggag gtatcgggaa cagccgcata
     3541 aaggtcaggg caaagatcat taaggcagat aaaaaaaccc tggtcatcaa ggagatcccg
     3601 tatgggcaga ctaccgacag tcttatccgt tccattatta ctgccaatga taaaggcaag
     3661 atcaagatca ggaaaatcga tgataatacc gctgaacagg tcgagatcct gatacacctg
     3721 gccaatgatg tttctcccga taaaaccatt gacgccctgt acgcctttac aaagtgcgaa
     3781 gtgtccattt cacctgatac ctgcgttatc atggatggaa agccctttgt gacgagcgtg
     3841 gagaacatgc ttggatacag tacggaaaga acccgtgatc tgctgaagag agagctggag
     3901 atccgcctgg acgagctgga ggataactgg catttttctt ctcttgaaaa gatatttttt
     3961 gaagaaaagg tttaccgtat cctggaaaac aatgcccgga cctgggaaga ccagctcaag
     4021 gacgtggaaa ccgaaatgct acgctaccag gatcgcctgc gccgtcctgt agtatttgcc
     4081 gaaattgaga aactggtgga aaaacctgta cggaaaatat cccgctttga tatcaagaag
     4141 gcaactgagc atattaaatc cctggacagg gaaatggaac agatcaggca caacctggag
     4201 catcttaccg catatgccat agagtactac gaaggattaa agaaaaaata cgggagcttt
     4261 ttccccagga agacagagat caccagtttc cagaccatag aagcttccaa tgtgatcctg
     4321 gccaactgca aattgtacgt cagcagggaa tcgggatttg tgggggctga tatgaaaaag
     4381 gatgacaagg cagaatacct ctgcgattgt tccagcctgg acgatgtcat ggtatttctc
     4441 aaagatggca cgtattcggt caggaaagta tccgataagc aatacgtggg aaaagacatc
     4501 atttatgccg ggatactgag aaaaaaagag aaccggacgg tatataatgc cgtttaccgt
     4561 gatgggaaaa acgggaaagt gttcgcaaag cgtttccaga tgaacagttt ttcacgtgac
     4621 agggattata acctgacaca gggaacaccg gggtcgcagg tattgtggtt atctgttaat
     4681 ccaaacggag aggcagagac cataagggtt ttcctgaagt cccgtccgaa attgaagaag
     4741 ctgatctttg agttctcttt cctggatctg gccatcaagg gacggagcag ccgagggaat
     4801 atcctgagcc gtcaccccgt caaaaaaatc accctcaagg ataaagggat tgccacactg
     4861 ggcggacagg aaatctggtt tgataaggat atacagcgtc tcaatgacca gggtagagga
     4921 ctatacctgg gtgaattcca cgccgatgac agcctgctgg ccattaaccg aaacggaaca
     4981 ttctgtacta cgaatttcga cctgagcaac cgctaccagg aagaggtact gtttattgag
     5041 aaaatggatc cggaaaaaac cttcagtgcc gtttactatg acagtgatgc cgaggcttac
     5101 tatgtcaagc gtttcaggtt tgagtcatct gaaaacatgg ttcagtcatt tatcggagaa
     5161 ggagagggat cttacctggt ggcaatttcc agtgacaaga atcccgttgt taaagtcaca
     5221 tttggaggaa aacacgaaaa aagacctccc caggaaataa atgtaaccga attcatagga
     5281 gaaaagagtt tccgggccaa ggggaaaagg atcaccactt tccaggtgga aaagatcgaa
     5341 ttctgtgaat ccgcttcggc gaatgaggat accttgccgg ataacgggga cgacggcagc
     5401 ctcgactttg aactggaggg tcccgtttcc aaaggagacg ttgcacctgt cccgtcggca
     5461 gataagaacc ctctggcaga tttcaatgac caggatgcca tccagatgat gctactgtaa
     5521 caatcatgcc atgggtctga ttttttacat aggtttcatg gcctcgggca aaagcaccct
     5581 gggagcagcc gatgcagccc gtgacgggta taaattcaca gacctggacc agaagctctg
     5641 ggacaacacc ggaattcctc catggcagta cataaccgaa aaaggagaag aagccttcag
     5701 gattgccgaa agggacatcc tgcactggat tgtggaggag tggcaccggg aagggcaacc
     5761 gcctcagctg gttgcctgcg gaggaggcac cccctgcgcc ttcgataaca tggaattcat
     5821 gaaaagtaac ggtcaagtta tctttattga cacccccctg tccacgatcc tggaaagaat
     5881 acggaacaat ccacacagat ggcctcttgc cgggatctca aacctggaat ccctttacct
     5941 ggaaagaaga aaatggtatg aaaaggcgca cgaacggaaa gtgccttaga caattacgtc
     6001 ccgtgtcttg atatctttga tgcgcagctg gatgtttgct atgcccctgt aatagttttc
     6061 tgctaccgaa taacatatat ccaccggatt cccgttatgc atatgctcga agtgttcgct
     6121 ttgattgaat gctatggcag atatgggacg gtaaggctcg tcttcctgga taagttccaa
     6181 tttcagatgg cctgaatctg accccacaag acgtccattc ccgttatcat acacgttctc
     6241 ggtaacaaat accggagaca ggttccccgg gccgaaaggc tgaaattgct ttagaagacg
     6301 gaaaaatttt ggggttatct gcctgaattc aatgtaggta tcaatacttt ccaggggggt
     6361 cagcatctct tctgtgactt tctccgccac gatgtcttca aaccgttggc aaaactcctg
     6421 gtaacgctct tctttcatgg tcagtccggc agcgtacatg tgaccgccaa agttttcgag
     6481 taaatcgctg cagttttcaa tggcctcgta aaggtcgaat cctggtatgc tccttgccga
     6541 tcccgttatg aatccgttgg acttggtcag cacgatggtg ggacggtaat aattttcgac
     6601 cagccgcgaa gcgacaatgc ccacaacccc cttatgccat ttcgggttgt ataccaccgt
     6661 ggatttcttg tccttcagac cgggcatgtc ccggaccatg tccagcgctt ccgcggtgat
     6721 cttgcggtct atgtttttgc ggtcatcatt gtggtcattg atggtattcc ctatctggga
     6781 ggcgtcgtca tccgttcttg atatgagcag gtcaactgcc atctttcctg attccatcct
     6841 gcctgccgca ttgatccggg gtccgatctt gaagacaata tcgtccactg tgatctggtg
     6901 cttttcaagg ccggccagct tgatgatcga gtgcaaccct ttccttggag cttcattgat
     6961 ctgtttcagc ccgtaatggg caagtatgcg gttttcaccg gttatggata ccaggtccga
     7021 ggcgatgctc accaccacca ggtccagaaa gggatagagt tcggtaagcg ggatattcct
     7081 gctcatgcaa aaagcctgca acagtttgaa gcccactccg caaccgctca gctcatcaaa
     7141 cgggtaatgg cagtcgttcc tttttgggtc gagcacagcc accgcctggg gcagttcatc
     7201 tgcaggaagg tgatggtcgc aaatgataaa atcaatccct ttgtgcctgg catactccac
     7261 cttttctgta gccttgatac cgcagtccag cgctatgatc agggtacagc cttcctcggc
     7321 tgctgcatca atgcctatgt aggatatgcc atacccttct gtgtaccggt cgggtatata
     7381 ataagatacc atgggtgttc ccttgcggag aaaggaataa acaagagcga cggctgtcgt
     7441 gccgtccaca tcgtaatcac cataaaccag gatcttttcg ctttcacgga tagccctgtc
     7501 aagtcttttg acggctttat ccatgtcttt catcagaaac ggatcgtgaa ggttttccag
     7561 ctggggacga aaaaaagatt ttgcctgtgc gaaatcctta acgtttcgtt gcaccagaag
     7621 gtttgccagg gcattatcaa ttccaagctc tgcaaccagc tgccttacca gcgcgggatt
     7681 tcccggttcc ttgacgatcc attttttttc cagtgccata attatcctgc aaaggtaaca
     7741 atataatcct tcccctctaa aaaaattctt atttttgtgg gccacataac tgtaagaaat
     7801 ggagaacaca catatcagtg aacaggaaca ggtccgtcgc gacgcattga ctgaaatcaa
     7861 aaaactgggt attgacccct ttcccgccgc aacatatccc gttgacacga cagctgctga
     7921 aatccttgag ggatacgatc ctgaaattga aaatttcaaa gcggtaacct tggcaggtag
     7981 gatcatgagc cgcaggatca tgggggctgc ttcctttatg gtcatccagg accacagcgg
     8041 acggatccag gtgtatgtaa aaagggatga tatatgtccg acagaagata aaacactcta
     8101 taacaccgtc tttaaaaaat tgctggacat aggggatata gtgggaataa ggggatttgc
     8161 atttatcacc atgacaggtg aactctctgt gcatgcccgg gagttggtcc ttctcagcaa
     8221 atccctgcgt gttctgccca taatcaagga aaaggaggga caggtatacg acgctttcac
     8281 agaccctgaa caacgttaca ggatgcgata tgtagacctg atcgtgaatc cccaggttag
     8341 ggaaacgttc gtgaaacgtt caaaaatggt gaacataatg cgtgactttt tcaacagcca
     8401 gggttacctg gaagtcgaaa cccccatcct gcagccaatc ccggggggag ccaatgcccg
     8461 tccgttcatc acccaccaca atgccctgga cattccgcta tatttaagga ttgccaacga
     8521 attgtacctg aaaaagctta ttgtaggtgg attcccgggt gtatatgaat tttccaaaga
     8581 tttcaggaat gagggcatgg atcgcaccca taaccctgag ttcactgtgc tggaaatcta
     8641 cgtggcttac aaggattacc tctggatgat gaacttcacg gaagaaatgg tggaaagaat
     8701 agcgctagaa ctccatggca gcacccgggt accttatggt gataatgtca tagactggaa
     8761 aaggcctttc aggcgcatct ccatgactga tgccataaaa gaatatgccg gcgttgatgt
     8821 cacggacatg gaggaggagg acttgcgcag gacctgcatg gatctgaacg tgcacattac
     8881 ccctttcatg ggaaagggta aactcataga tgccctcttt ggagaattct gtgaaaagca
     8941 cctgatacag cccactttca ttaccgatta ccctgtctcc acatcgcccc tgactaaaag
     9001 acaccgtgaa aagctccacc tgacagaacg tttcgaactt tttgtaaacg gaaaggaact
     9061 ggccaatgct tattcggagc tgaatgatcc gctggaccag ctggagcgtt tcaaagaaca
     9121 actcaggctg aaggaaaagg gagatgatga agccatgttc atagatatgg actttatcag
     9181 ggccctggaa tttggcatgc cgcctacctc gggtatggga atcggaatag accgtctttg
     9241 catgctgatg accaatgcgc caagcataca ggatgtgctc tttttcccgc agatgcgtcc
     9301 cgaatgaatt atcatgatat cttctcccaa taatccgaaa atcaaacagt tgcgtctgtt
     9361 aatggaaaaa tcgcgagtgc ggcgggaaaa gggattgttt gtcatagaag ggataaggga
     9421 gttggagatt gcactgaaaa gcgggtacat cattcaagaa ctctacctgt gtcctgccat
     9481 tttgcaggat gcggaaaagg cgtcctggtt cagggagcat tccctgatat cagatgacct
     9541 ggaagccggc ctgtacggaa aactggctta tcggggaaca acagaggggg tactggccgt
     9601 catgcgtaca cggaaaatga aacctgaaga aatactcctg caggaaccgg ccctggtcct
     9661 agtgctggaa tctgttgaaa agccaggcaa cctgggtgcc atactgcgaa cggcagacgc
     9721 atcgggtgcc ggagcggtcc tgatctgcga cccgctgact gacttgtata atcctaatac
     9781 cataagggct tcccttggct gtatattcag tgttcccacc ctggcctgta cttctgacga
     9841 agcatataaa tggcttagca ggcagggtat tcaggtggta gctgccacgt gccaggccac
     9901 atcctggtac cacgaatgcg attacaggaa acccaccgcg ttggtgctgg ggtcagagag
     9961 cgatggcctg cgccctttct ggagggaaaa ggcagccaaa gaagtgaaaa tacccatgat
    10021 ggggatcatt gattcactga acgtttcggc agcagcagcc attctctgct ttgaagcgct
    10081 caggcagcgc aatgcatgaa catgggacct ttcgcaatca ttggaaatcc ggttacatcc
    10141 agccttagtc ctcgcctttt cagcgcagcc tacccgcaat tgccccgcga aatgtatctt
    10201 gtgattgaag cacccgatgc ggaacagggt tggaaacgca taaaagagct gcatatacaa
    10261 ggtttcaacg taaccatgcc tttcaaaacg gattttctga acatggctga caagctcgct
    10321 cctgcagttc gacaagtccg tgccaccaac ctgttaacct tggaagacgg tatatggaca
    10381 gcctggaata cagatattac aggtgttgtc aacagcttca ggggatccgg gattgatccg
    10441 gcaggaaaaa atgctttgat cataggtgca ggtggcgccg gacgggcagc tgccctgggt
    10501 ctggaacaag ccggggcgac agtttttata gcaaacagga cactgcggcc gagattgtat
    10561 ccgctgtcgg atatcccttc cctgcttaga aaatgcaccc ttatagtcaa tacggttccc
    10621 tgtgaaggca tagatgctgc agttataaat ctcacgtgcg cacatacaat actggatgcc
    10681 tcctatacac tggctcctct aaggaaggct gctgcctgcg cgggcgcccg ttacctgggc
    10741 ggatatcact ggctgtacaa ccaggccgtt gaaggctttg ccattttaac cggacttgag
    10801 ccggattgca gggccatgcg caagctgctg gatttataaa aaagattgta cctttacaaa
    10861 gtatataaac caataaacaa tcataatact atgttgaata aggtaatgtt aataggaaat
    10921 gt
//
LOCUS       DAOSOF010000058        10707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6153_length_10707_cov_5.449024, whole genome shotgun sequence.
ACCESSION   DAOSOF010000058 DAOSOF010000000
VERSION     DAOSOF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6153_length_10707_cov_5.449024"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLM68_04635"
     CDS             complement(<1..334)
                     /locus_tag="PLM68_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54683.1"
                     /translation="MRRFPDIFLCLLLGLSFLAGKAGLTVHHCLREGSSHVVLLSADP
                     SCRAVHAQQAGHTPEADCTRDNSASHGSCERIHDQDCCSTEVYFLEDPSLVSDGLDTP
                     APDSTRITA"
     gene            389..2143
                     /gene="sppA"
                     /locus_tag="PLM68_04640"
     CDS             389..2143
                     /gene="sppA"
                     /locus_tag="PLM68_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT54684.1"
                     /translation="MKDFFKTLAAVILGSALVFISGFFLLMGSLTTMISMGLGGVTPS
                     IPRNAILHITFEEGVATQDSDLPSFGFLPPELDIRQGGTGFLGIVHAIEQAAEDPSVR
                     MIFLDPQYLDAQMTHIEEIRNALMTFRKSGKPVISYAGAYSQQAYYLASAADKVIINP
                     AGSITLQGFSVKVSYYRELFDKLGLEAQVIRHGNYKTGGEPFTAFRMSEQERTQMSAY
                     LSAAWKHWSENMEQARHMEEGTIDRMSARSFITDAQHAKEMGLADEVWHQDEMISYLS
                     SVYEGLPERKLPFLPLQHYIKYMELNRQKPVKDRIAVVYASGDIAMGKGTGQVFADAY
                     AAQLRKYRSDSTIRAVVIRVESPGGDPMAAEIITREIRLTADTKPVIVSMGDMAASAG
                     YWIASAASRILVQPSSMTGSIGVYSIFFNGEKTLSDIVGVRSETIDTHPFSHFNSLYH
                     RKSEQELQIVREQIESTYDRFVLTVSENRHMEKQSVENLADGRIWSGTDAISNGLADQ
                     QGGLSDAIRMAAGMAGVADYRLVSYPKPFRIIDLIPIGGRKNNPGIEGLIQRELLDLL
                     GEGGIRARLPFDERAISW"
     gene            complement(2058..3413)
                     /gene="tilS"
                     /locus_tag="PLM68_04645"
     CDS             complement(2058..3413)
                     /gene="tilS"
                     /locus_tag="PLM68_04645"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT54685.1"
                     /translation="MRTLEHVFGEAAGRLLEGSSGGILLAISGGADSMVMAHLFFQWF
                     GRGEEKQMNRRKAVAHMNFSLRGSDSDGDQSFVEAWARGKDIPFYAKKVDTLAYASEK
                     GISTEMAARELRYAWFEWLCREHGLSHIAVAHNANDRAETMLLNLVRGTGLRGLCSLR
                     EQNGKIIRPLLEVPRKAILDYARENHIDYRTDATNQETVFARNKIRHLVMPVLEEITP
                     NVTLRMGKNADNLLQVQCLLDSLTMEKRRACATSRGLSISCLVQGGNVEFWLYQLLSP
                     HGFNASQITGIARAMEGQSGKKFLSPSHILWIEREELVIRELADTLTLAKLGFEKVEK
                     TPDLIIPQDRNIAALDANTLEFPLEVRRWKPGDKFMPLGMKGFKKVSDFLIDQKVPAG
                     DKKEICVLCSGSNIVWVIGWRIDNRYRVTDNTTTILLFHQREDVPGSLPLPEDQAILS
                     E"
     gene            complement(3417..4481)
                     /locus_tag="PLM68_04650"
     CDS             complement(3417..4481)
                     /locus_tag="PLM68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOT54686.1"
                     /translation="MKKIAFLAVVFLMATGIASAQEKVRVDSLQKDSVVIPTYEELTR
                     MVFRLTAKVDSARQRAIEDSILIARFIEESRQAALASMPPPKKPTFWTNSLLSQLNFS
                     QTSLTNWAAGGNSSIALGGYVDAKANYEKDKILFQNRFQIGYGFVKMFGDILKKTDDR
                     LLLDSKFGYKATEKLYFSAAFGMKTQMAKGYKYAKTDTTQVSQFAAPLYSTLALGIDY
                     KPYSWLSVTFAPLTGGIVVVTNPDLRETYGNKKDEKVRKEFGAQLKVKLDKEVFKNVK
                     VDTELILFSDYLNKPQNIQVYWDLSVSMQVNKFLSANIRTNLIYDDNIKIADKTGLKA
                     ARVQFREVLSIGLSYTISNK"
     gene            complement(4494..5204)
                     /locus_tag="PLM68_04655"
     CDS             complement(4494..5204)
                     /locus_tag="PLM68_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54687.1"
                     /translation="MTNSEIRPTRKQIIQGLNEKSRMKNKVFLQTLEVFNVVKEVLHE
                     MSNDVNELLEDGENRRVRFEYRDRGKFEAELKFADDILIFSMHTDVFQFDRDHPVWKK
                     DYVKKDPFATYCGMISVYNFLTDSFKYNRKDDLGYLIARVFINKDRSYFTEGKRQQTR
                     DLSDFGKRSIDKAAIVNIVETAVLYCLSFDLLAPPYETVALATVEQMNAKIDSAKMQT
                     GKRLGFAFCADDVKADVE"
     gene            complement(5213..6685)
                     /gene="proS"
                     /locus_tag="PLM68_04660"
     CDS             complement(5213..6685)
                     /gene="proS"
                     /locus_tag="PLM68_04660"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT54688.1"
                     /translation="MAKEIKNRDENYSEWYNNLVIKSELAENSAVRGCMVIKPYGYAI
                     WEKIQAGLDRRFKETGHVNAYFPLFIPKSFLSKEAEHVEGFAKECAVVTHYRLMANPE
                     GKGLVVDPDARLEEELVVRPTSETIIWNTYRNWIKSYRDLPVLCNQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATREEAMEETRKIVNLYADFAREVMALPVWIGPKTASERFP
                     GAVETLCIEALMQDGKALQAGTSHFLGQNFAKAFDVQFANKEGRLEYVWSTSWGVSTR
                     LMGALIMAHSDNHGLVLPPALAPIHVVLVPIYKGEDERTSICEHLKTISTRLQALGLT
                     VKLDDRDNVRSGFKFSEWEVKGVPVRIAVGPRDMSLGTVEVARRDTLEKQIIPLERID
                     EFIPRLIKDIQQNIYDKALDFRNRNTFEIDSYDEFRERIEEGGFFLCHWDGTAETEAK
                     IKEETKATIRLIPECEDPRPGKCMYTGKPSPQRVVFARAY"
     gene            6801..8159
                     /locus_tag="PLM68_04665"
     CDS             6801..8159
                     /locus_tag="PLM68_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54689.1"
                     /translation="MKKFFPYLLMVTGLLAVSCGSGYQVVSSRYDDATYFRPDVTARV
                     HMLATASEAEALINETLAKAKQEGTKVETVYTDPQGVANINVEPGTTYIVMDAQDDSY
                     ERKLEMFGDDPDDNFSLTINMSFNYGDWWEYPFYDYPWYGGWNRYRYWYMPMSYWDSW
                     YWYPRTWYRNFWYNDWFYNYWYYPSWYHMWSYSGIWGIYPYGYHDKYRDYRNDRRDNS
                     NRRDYSNNRRQDLGRNAPSSISGGSIRSGSSYARTNPKIEQVSGNRDQRTRDNIENPV
                     DNKTDIRSESGTTNYRRVTGTTGSSAVYRDPASTTKSRTEGTPDQNTYYRRSSNLSRE
                     NTRTESSATTTNPGRSRSFYRETQKPSTTVNSGSAGSSRSVRSSSGSTSGSRSTGSYD
                     SGSSYRSSSSSSATKSSSSVSSGPSRSSSIGSSISSGASRSSTGGNSGASRSGSGGSY
                     RR"
     gene            8196..9725
                     /locus_tag="PLM68_04670"
     CDS             8196..9725
                     /locus_tag="PLM68_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54690.1"
                     /translation="MKKLLILIALMAPFALAGQNAWDAMRYSQVNYQGTARSLALGNA
                     VTALGGDFGSITLNPAASAVYPYSEISITPSLVTSFSQVDYYSQRTDDRYTRPGLSSL
                     GYTGSWSTSNSKGLVSLSFAAGYNKIQDFTMRTSVRRNNSESSWLTPVATSTNGIVYS
                     ALEHGDGYNPYYESNASWRSVLAWNTYLLDTLPGTLDQYIAAPENLYGEEIVVGGPLD
                     QRFSRETKGSMGEYTLNMGANISNRFFIGYNFNFRNVFYRTYEKFTETSQEPEDFQTL
                     FSSFTHTYEQTTTGLGFNMSLGIMAIPVSLPGFNWRLGASISTPTWTSLTDEWQEYMT
                     ADFRDGQKNASSPVSSYSYKVRTPWRFRLGTAFIIGKLGLISFDYEGTDYRDMRMSNK
                     YDKWAFEQDNEAIRYGSETFGFQYAHNFRAGAEIRIAQLALRGGYSYYGAPEQSYEAT
                     HMVSGGVGLRGKQFFTDLGLCYRLNESEGFSLYDNGTPGTPGLNTLSQLRILFTFGVR
                     F"
     gene            complement(9681..10304)
                     /locus_tag="PLM68_04675"
     CDS             complement(9681..10304)
                     /locus_tag="PLM68_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOT54691.1"
                     /translation="MRAFFTTSYWPPIACFAALYPDGELVLEQHEHYQKQTIRNRCSI
                     CGANGPQWLIIPVLRRHGTKTPIREVMLDLSSDWQRVHWLAIESAYRSSPYYEYYRDE
                     IRKFYTLKKSISLIEYNSAILRWTAEALETGISVSYTESYNTSYSELDFRNHLPAYEP
                     VPYYQVFARKHGFVPGQSILDLLMNEGPEAWKALKPNTKGEQDPQLR"
     gene            complement(10312..10617)
                     /locus_tag="PLM68_04680"
     CDS             complement(10312..10617)
                     /locus_tag="PLM68_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54692.1"
                     /translation="MISGGSLIGKDVPPYVLAGRRPLCYGGINIIGLRRRGFNSEQIE
                     RIHDIYRIIYQVGLNISDACRKVEEVLDVSEERNVILSFIRASQRGIIPHKAKFMEE"
BASE COUNT         2657 a         2724 c         2550 g         2776 t
ORIGIN      
        1 aggccgtaat cctggtggag tcgggggcag gggtgtccaa tccgtcagaa accagtgagg
       61 gatcttccag gaaatacacc tctgtactgc aacagtcctg atcgtgaatt cgttcacagg
      121 agccgtggct ggcggagttg tcccttgtgc agtctgcttc cggagtgtgt cctgcctgct
      181 gagcatgtac cgccctgcag gagggatcgg ctgacagcag caccacatgg gaggaaccct
      241 cccgcaggca atggtgaaca gtaagtcctg ctttccctgc aagaaacgac aggcccaaca
      301 aaaggcataa aaaaatatcc gggaatcttc tcattctggt ttgcaaatat aatgttttat
      361 ttttgtatga atatctaaaa atacggttat gaaagatttt ttcaagactc tggcagctgt
      421 tatcctggga agtgcacttg ttttcatttc cggtttcttc cttctgatgg gctcactcac
      481 cacgatgata agcatggggc tgggcggcgt gacgccttcc ataccccgca atgccatatt
      541 gcacataacc tttgaagagg gtgtcgccac ccaggactcc gatctgccat cgttcggctt
      601 cctgccccct gagctggaca tcaggcaggg aggtacaggc tttctgggca tcgtgcatgc
      661 catagaacag gcagctgaag atccttcagt caggatgatc ttcctggacc cccagtatct
      721 ggatgcacag atgacccata tagaagagat ccgcaatgcg ctgatgacct tccgtaaaag
      781 cggaaagccc gttatcagtt atgcaggggc ctattcccag caggcatatt acctggcaag
      841 tgctgccgac aaggtgatca tcaacccggc aggaagcatc acccttcagg gtttcagtgt
      901 gaaagtctct tattacaggg agctgtttga caagctcggc ctggaagcac aggttatccg
      961 ccacggaaac tacaaaacag gtggtgagcc atttaccgct ttccgcatga gtgaacagga
     1021 aagaacacaa atgtctgcct acctgtctgc tgcctggaaa cactggtcag agaatatgga
     1081 acaggcccgg cacatggaag aaggaaccat tgaccgtatg agcgcccgct cattcataac
     1141 agacgctcaa catgcaaaag aaatgggtct ggcagatgaa gtgtggcatc aggatgagat
     1201 gatctcttat ttatcttccg tttatgaagg attgcccgaa aggaaactgc ccttccttcc
     1261 gcttcaacat tatatcaagt atatggagct gaacaggcag aaaccggtta aggacaggat
     1321 cgctgtcgtc tatgccagcg gtgatatagc catgggaaaa ggaaccggac aggtttttgc
     1381 cgatgcttat gctgcacaac tgaggaaata caggagcgac tccaccatac gggcggttgt
     1441 gatccgtgtt gaaagccccg ggggcgatcc catggctgcc gaaataatca cacgtgaaat
     1501 ccggctgacg gctgacacaa aacctgtcat tgtatccatg ggggacatgg cagcctccgc
     1561 agggtactgg atcgccagcg cggcttcccg catactggtg caaccttcaa gcatgacagg
     1621 atcaattggg gtttacagca tctttttcaa cggggaaaaa accttgtccg atattgtggg
     1681 cgtccggagc gaaaccattg acacccatcc tttcagccac ttcaattccc tgtatcaccg
     1741 gaagagtgaa caggaactgc agattgtcag ggaacagatt gagtcgacct atgaccgttt
     1801 tgtcctgacg gtgtccgaaa acaggcacat ggaaaaacag tctgtggaaa atctggccga
     1861 tggtcgcatc tggtcgggaa cggacgcaat cagcaacggc ctggcggacc agcagggcgg
     1921 tctgtcagat gccatcagga tggcagccgg tatggccggt gttgctgact acaggttggt
     1981 cagctacccc aaaccattca ggataataga tttgatccct attgggggaa ggaaaaacaa
     2041 tcccgggatt gaaggcctta ttcagagaga attgcttgat cttctgggag agggagggat
     2101 ccgggcacgt cttccctttg atgaaagagc aatatcgtgg tagtattatc tgttaccctg
     2161 tagcggttgt ctatacgcca tcctatgacc catacgatat tactgcccga gcatagaaca
     2221 cagatttctt ttttatctcc ggcggggacc ttctgatcga tcaggaagtc gctcaccttc
     2281 ttgaatccct tcattcccaa gggcatgaat ttatccccgg gtttccaccg ccggacctcc
     2341 aggggaaact cgagtgtatt ggcatccagt gcggctatat tccggtcctg cggaatgatg
     2401 agatcaggtg ttttttccac cttttcgaag cccagttttg caagcgtgag ggtatcggcc
     2461 agttcccgga tgaccagttc ttccctttca atccagagta tgtgggaggg agacaggaac
     2521 tttttgccgc tctgtccctc catggccctg gcaatgccgg ttatctgtga ggcattgaat
     2581 ccatggggag agagtagctg gtacaaccag aactccacat ttcccccctg taccaggcag
     2641 gagatggata aaccccttga agtggcacag gcacgacgtt tttccatcgt cagggaatcc
     2701 agcaggcatt gaacctgtaa caggttatct gcatttttcc ccatgcgtag tgtcacatta
     2761 ggagttatct cctccagaac gggcattacc aggtgacgaa tcttgttgcg ggcaaagaca
     2821 gtttcctggt tggtggcatc tgtcctgtag tctatgtgat tttcccgggc atagtccagg
     2881 atggccttac gcgggacctc aagcaatggc ctgatgatct tgccgttttg ttctctcagg
     2941 ctgcacagcc cgcgcagccc ggtccccctg accaggttca gtaacatggt ctctgccctg
     3001 tcattggcat tgtgtgccac agctatatgt gacaggccgt gttccctgca gagccattcg
     3061 aaccacgcat accgcagctc cctggctgcc atttcggttg atatcccttt ttccgaggca
     3121 taggctaggg tgtcgacttt ttttgcataa aagggtatgt ccttcccgcg ggcccaggct
     3181 tcaacaaagc tctggtctcc gtcactatct gagcctcgca ggctaaaatt catgtgggcc
     3241 actgccttcc ttctgttcat ctgcttttct tccccgcgtc caaaccattg aaaaaacaaa
     3301 tgagccatca ccatggagtc tgctccccca ctgattgcca gcaggatccc tccggaggat
     3361 ccttccagca acctcccggc agcttcccca aatacatgct caagtgtcct caatgattat
     3421 ttgtttgata tggtatagga aagtccaatg gacaacactt cccggaactg cacccttgcg
     3481 gccttaagcc ctgtcttatc tgcaatcttg atgttgtcat catagatcag gttcgtacgg
     3541 atgttggcac tcaagaattt attaacctgc atggacacgg aaaggtccca atacacctgg
     3601 atattctggg gtttgttaag ataatcggaa aaaaggataa gctccgtatc aaccttgaca
     3661 tttttgaata cttctttgtc aagctttact ttcagctgtg ctccaaactc tttgcgaact
     3721 ttttcgtctt tcttgttccc gtaagtttca cgcaaatccg gattggtgac cacaacaata
     3781 cccccggtca atggcgcaaa tgtcaccgat agccatgaat aaggtttgta atctataccc
     3841 agggccagtg tagaataaag gggcgcggca aactgcgaaa cctgtgttgt gtcagtttta
     3901 gcgtatttgt aaccttttgc catttgtgtt ttcataccga atgctgctga gaaatagagt
     3961 ttctccgtag ctttgtaacc gaatttagaa tccagcaaca acctgtcatc tgttttcttg
     4021 agaatatccc caaacatttt tacaaacccg taacctatct ggaaacggtt ctggaacagg
     4081 attttatctt tttcataatt ggccttggca tctacatacc ctcccagggc aatcgagctg
     4141 tttcctcctg cagcccagtt ggtaagcgat gtctgtgaaa agttcagctg ggaaagtaag
     4201 gaattggtcc agaaggtggg ttttttggga gggggcatgc tggcaagggc tgcctggcgg
     4261 ctctcttcaa taaaacgggc tatgagtatc gagtcttcaa tggccctttg cctggcagag
     4321 tcaactttgg cagtgagcct gaaaaccatc cgtgtcagtt cttcataggt cgggatcaca
     4381 acagaatcct tctgcaaaga atcaaccctt actttttcct gggcgcttgc aattccggtt
     4441 gccatcaaaa agacaactgc gagaaatgca atctttttca taatagtatg attttattca
     4501 acgtccgctt tcacatcatc agcacagaag gcaaaaccca gtcgcttgcc tgtctgcatt
     4561 ttggcactgt ctatcttggc attcatctgc tctaccgttg ccagggcgac cgtctcatag
     4621 ggaggtgcca gcagatcaaa agacagacaa tacaatacag ccgtttccac tatgttcaca
     4681 atggctgcct tgtctatgga acgctttccg aaatctgaca ggtcccttgt ctgctgcctc
     4741 ttgccttccg tgaaataact cctgtccttg ttgatgaaca cccttgcaat caggtatccc
     4801 aggtcgtctt tcctgttgta tttgaatgaa tcggtaagga aattatagac agatatcata
     4861 ccgcaataag tggcaaaagg atcctttttc acgtaatcct ttttccagac gggatggtcc
     4921 ctgtcaaact ggaagacatc tgtatgcatg ctgaaaatga gaatatcatc tgcaaatttc
     4981 agttcggcct cgaacttgcc tctgtccctg tattcaaaac gaacgcgccg gttttcaccg
     5041 tcttccaaaa gttcattcac atcgttgctc atttcgtgca gcacttcctt gacaacgttg
     5101 aatacctcca gggtttgcag gaatacttta ttcttcatcc tggatttctc attcaaacct
     5161 tgaatgatct gctttcttgt ggggcgaatt tctgaattgg tcataataat atttaataag
     5221 ctctggcaaa tacgaccctt tgcggtgaag gttttccggt gtacatacat ttgcccggcc
     5281 gtggatcttc acactctggt atgagccgta tggtagcctt agtttcctct tttatctttg
     5341 cttcggtttc ggcagtgcca tcccaatggc aaagaaagaa ccctccttct tcaatgcgtt
     5401 cgcggaattc gtcgtaactg tctatttcga aggtgttcct gtttctgaaa tcaagtgcct
     5461 tatcatatat gttctgctga atatccttaa taagccgtgg aatgaactca tctatccttt
     5521 cgagcggaat gatctgtttt tccaatgtat cgcgccgtgc aacctcaacg gttcccagcg
     5581 acatgtccct tggaccaact gcaatccgga ccggaactcc cttgacttcc cattcggaaa
     5641 acttgaaccc ggaccgtacg ttatccctgt catccagctt tacagtcaaa ccaagcgcct
     5701 gaagccgtgt actgatcgtt ttcaggtgct cacatatgga agtcctttca tcctcgcctt
     5761 tataaatggg gaccaaaacc acgtgtatag gagccagggc aggaggcagg acaagaccgt
     5821 ggttgtccga atgagccatg atcagggctc ccatcagccg tgtggacaca ccccagcttg
     5881 ttgaccatac gtattccagc cggccttcct tgttggcgaa ttgcacatca aaggctttgg
     5941 caaaattctg tcccagaaaa tgtgatgtgc cggcctgcaa tgccttacca tcctgcatca
     6001 acgcctcaat acatagggtt tcaaccgctc ccggaaaccg ctcggaagct gtctttggtc
     6061 ctatccatac cggaagcgcc atcacttccc tggcaaaatc ggcgtacagg ttcacaatct
     6121 ttcgcgtctc ttccatggcc tcttcccttg tagcatgggc agtatggcct tcctgccaca
     6181 ggaattcagt ggtacgcaaa aaaagccttg tcctcatctc ccaccggacc acattcgccc
     6241 attggttgca aagcacaggc agatcacggt atgattttat ccagttccta taggtgttcc
     6301 aaatgatggt ttcgctcgtg gggcggacaa ccagctcttc ttccaggcgg gcatccgggt
     6361 ccaccaccag tcctttccct tccggattgg ccatcaggcg gtaatgcgtt accacggcac
     6421 attccttggc aaacccttcc acatgctcgg cttctttgct caaaaaggac tttgggatga
     6481 acaggggaaa ataggcgttc acatgacctg tttccttaaa acggcgatcc agtccggcct
     6541 gtattttttc ccatatggca tacccgtaag gttttatgac catacaacct cttacagctg
     6601 aattttcagc cagttccgat tttataacca ggttgttata ccactcagaa tagttctcat
     6661 ccctgttctt tatttctttt gccatgccat ccttgttttt ggtacaaaaa ttgtgtgttt
     6721 tattgttgtt ttaataatgt ttttgtatat ttgaaacaaa agtacataat ttgccacaat
     6781 aaaaaattag gaggtgcaaa atgaaaaagt tttttcccta cttactgatg gtaaccggac
     6841 tactcgcagt atcctgtggt tcaggttatc aagtagtatc ctccagatac gacgatgcta
     6901 cctacttcag accggacgtt accgcccggg tacatatgct ggctactgcc agtgaggcag
     6961 aagctctaat taacgaaacg ctggccaagg ctaaacagga aggcacaaag gtcgaaaccg
     7021 tttataccga cccacagggt gttgccaaca taaatgtcga gccgggaacc acctatattg
     7081 tcatggacgc acaagatgac tcatacgaac ggaagctgga gatgttcggc gacgacccgg
     7141 atgacaattt ttccctgacc atcaacatga gttttaatta tggcgactgg tgggaatatc
     7201 ccttctatga ttatccctgg tacggcggct ggaacagata caggtactgg tacatgccca
     7261 tgtcttactg ggatagctgg tattggtacc cgcgcacctg gtacaggaat ttctggtata
     7321 atgactggtt ctacaactac tggtactatc cttcatggta tcacatgtgg tcatactcag
     7381 gaatatgggg catctatccc tatggatatc atgataagta cagggattac aggaacgacc
     7441 ggcgggataa cagtaaccgt cgggattaca gtaacaacag aaggcaggat ttaggccgga
     7501 acgcgcccag ttccatatct ggcggttcaa tccgctcggg aagttcctac gccaggacca
     7561 atccaaagat cgaacaggta tcggggaaca gggaccaaag gacaagggac aatattgaaa
     7621 atcccgtgga caacaagact gacatcaggt cagaaagcgg taccaccaat tacaggaggg
     7681 tgaccgggac caccggttct tctgccgtat acagggaccc tgcttcaacc acaaaatcac
     7741 gcacagaggg cacccctgac caaaacacct actaccgcag atcttccaac ctgtctcggg
     7801 agaatacgcg tacagaatct tctgccacaa ctaccaaccc gggaagaagc cgcagttttt
     7861 acagggaaac acaaaagcct tcgacaactg ttaattcagg cagcgcagga agctcaaggt
     7921 ctgtccgctc ctcttcgggc agcacttcag gcagccgttc cacgggaagc tatgacagcg
     7981 gctcttcata caggagcagt tcttcctctt cagctaccaa gagcagcagt tctgttagtt
     8041 ccggcccttc aaggagcagt agcataggca gttctataag ttcgggtgct tccaggagca
     8101 gcacaggcgg aaacagtggt gcctcccggt ccggttccgg cggttcatac cgcaggtaaa
     8161 ccgggatatg aataaaccta tttgattcca taacaatgaa aaagctctta atactgattg
     8221 ctttgatggc tccctttgcc cttgctggac agaatgcctg ggatgccatg cgttattccc
     8281 aggttaatta ccagggaacg gcacgctctc ttgcactggg taatgccgtg acagcgctgg
     8341 gtggcgattt tggttccata accctgaatc ctgctgcatc tgccgtgtat ccatactctg
     8401 aaatttccat aactccttcc ctggtcactt cattttccca ggtcgattac tatagccaga
     8461 ggaccgatga ccgttacacg cgtcccggat tgtccagcct gggatacacc gggtcatgga
     8521 gcacctccaa cagcaagggg ctggtatccc tgtcttttgc agccggatac aacaaaattc
     8581 aggattttac gatgcgcacc tccgttcgcc gcaataattc cgaatccagc tggctcaccc
     8641 ctgtcgccac ttcgaccaac ggcatagtct attcagcgct tgagcatgga gatggctaca
     8701 atccctatta cgaatccaat gcatcctgga gatccgttct ggcatggaat acgtacctgc
     8761 tggacacgct gcccggtacc ctggatcagt atatagcggc ccccgaaaat ctctacggag
     8821 aggaaatcgt tgtaggagga ccgctggacc agcgcttttc ccgggaaacc aaaggcagta
     8881 tgggtgaata cactttgaac atgggggcca atataagcaa tcgctttttt atcggttaca
     8941 actttaattt cagaaatgtg ttctacagga cctacgaaaa gttcacagaa acctcacagg
     9001 aacctgaaga cttccaaaca ttgttttcat cttttaccca cacctatgaa cagaccacta
     9061 ccggtctggg tttcaacatg agcctgggaa taatggccat accggtaagc ctgcctggtt
     9121 tcaactggag gctgggggca agcatctcca cacctacatg gacatctctg accgacgaat
     9181 ggcaggaata catgacagcg gatttccggg acgggcaaaa aaatgccagc agcccggtaa
     9241 gctcctattc ctataaggtg cgcaccccct ggcgtttcag gttaggtaca gcattcatca
     9301 taggcaaact tggcctgatc agtttcgatt atgaaggaac tgattacagg gatatgagga
     9361 tgtcaaacaa gtatgataaa tgggcctttg aacaggataa tgaggctatc cgttatggat
     9421 cagaaacgtt tggtttccag tatgcccata atttccgtgc cggagccgag atccgcattg
     9481 cacagttagc cttgaggggc gggtacagct attacggtgc cccggagcaa agctatgaag
     9541 ccacacatat ggtttccggc ggtgtgggtt tacgtggaaa acagtttttc acagacctgg
     9601 gcttatgtta ccgtctgaat gaaagtgaag gtttttcgtt gtatgataac gggactccgg
     9661 gaacaccagg gctcaacacc ttatcgcagc tgaggatcct gttcaccttt ggtgttaggt
     9721 tttaaggctt tccaggcttc agggccttca ttcatgagta aatccagtat actttgcccg
     9781 gggacaaagc cgtgtttccg ggcaaaaacc tgataatacg gaacgggttc ataagcaggc
     9841 aggtggttac ggaaatcaag ctcactgtat gatgtattgt aggattcagt gtaggaaacc
     9901 gagattcctg tttccaatgc ctcggcagtc cacctgagaa tggctgaatt gtattcaata
     9961 agagaaattg acttcttaag ggtgtagaat tttctgatct cgtcccggta gtattcataa
    10021 tagggagaag agcggtaggc agattctatg gccagccagt ggacacgctg ccagtcactt
    10081 gacaggtcaa gcataacctc ccggataggt gtcttggtgc catgcctgcg caatacagga
    10141 atgataagcc actgaggtcc gttggccccg catatggaac agcggttccg gatggtctgc
    10201 ttctggtaat gttcgtgttg ctccagcacc aattccccgt caggatacaa agcggcaaaa
    10261 caggctatcg gaggccaata tgatgtggta aaaaaggccc tcatggtctc attactcttc
    10321 catgaatttt gccttgtgag gaatgatccc ccgttgtgaa gccctgatga atgacaatat
    10381 gacattcctt tcttccgaga catccagtac ttcttccacc ttgcggcagg catcactgat
    10441 attcagcccg acctggtaaa tgatgcggta aatatcgtgt atcctttcaa tttgttccga
    10501 gttgaacccc ctgcgacgca ggcctatgat attgatccct ccgtagcata gcggacgcct
    10561 tcctgcaagc acatatggcg gcacgtcttt ccctatcaag gatccccccg agatcatggc
    10621 ataggctcct atgcgtgtga actggtgagc tacagatgct ccgccgatga tggcatattc
    10681 atcaatattg gtctcacctg ccagtca
//
LOCUS       DAOSOF010000059        10540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6304_length_10540_cov_4.176824, whole genome shotgun sequence.
ACCESSION   DAOSOF010000059 DAOSOF010000000
VERSION     DAOSOF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6304_length_10540_cov_4.176824"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLM68_04685"
     CDS             <1..262
                     /locus_tag="PLM68_04685"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT54693.1"
                     /translation="RLTIALEAGNMAWWEMELTLGKIVFSHNKTRMLGLKGEDFNHFQ
                     DFIKIVHPDDRDTTMNAMYDHLRRDAEMYECEYRMKNAEGNY"
     gene            465..1367
                     /locus_tag="PLM68_04690"
     CDS             465..1367
                     /locus_tag="PLM68_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54694.1"
                     /translation="MVGFASLLHQQNLDTETRNPYIDIIESSSRQLLNLVNDIIDISR
                     IEAGELKINKTPFELHKILKETETLFGKLKHARGKQNLKIKLDIPERDQVLYIVTDPD
                     RLKQILINLIGNALKFTEKGGIDFGYRIEDDRLKFYVSDNGIGMTEDDLRLVFERFKR
                     SEQAHRKYEGTGLGLAITKELLDLLGGSISVESQLGRGSVFTFRIPYVPAKSQEATHN
                     NEQTTDLERLRGKKSLIAEDEKVNRMYLEIILKELPIDIYWAKTANALSSDREKYLES
                     GFVDHISKPIDRLQLIRIMERWAL"
     gene            1459..2535
                     /locus_tag="PLM68_04695"
     CDS             1459..2535
                     /locus_tag="PLM68_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54695.1"
                     /translation="MSVIHLFKKAAVIATALVFVFTLQGCPYIDPDHDYEFEYEIIIT
                     DVPANLEGFNSQYNDYNSALPFEANRREIYFSTDRKTNGSTYDIISHAFDISYHKRDD
                     VLNISIPVNPGESIMDRKLLPLINTGYDELGPYTYFEDGGLNYFFYAGNGSDEQEEPG
                     DYDIKFVYMPRSDWTTYGGQQRLFGPYDVSLANSEADDLYPVISDKLGKMLFCSNREN
                     DSFDIYSLELEQGALLNQFLTGNYEPVIAKEPALSGEGHDKCPSIFGDYVIFASDREG
                     GYGGFDLYYARYENNAWSEPVNFGDKINTASDEYRPLGFKFVDFSLMIFSSDRPGGKG
                     GFDLYCVDVSDIIEVQDWYRFYEF"
     gene            2592..3824
                     /locus_tag="PLM68_04700"
     CDS             2592..3824
                     /locus_tag="PLM68_04700"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOT54696.1"
                     /translation="MVSQGYTTGRQPRAAGSSAWAIEQHSVAAAGPGADTGSRNEGTC
                     VGKLFYKKGTPGEVWIALLEQFNIVAVYANEDYEPYAIARDSEIEGLLAQKGVLFHLC
                     KDQVTFARDEVVKKDRTPYTLFTPYKNAWIKKIAELHVKPIQNLLEDPLHEPLPARLQ
                     TGLPARLQTVLPARLQTGLPARFAEFSFPFPSLTGIGFKRSSLKVRDYNIDNLDNYDK
                     DRNYPALDGTSHFSVHLRFGTVGIRSIMSHIQHNESFLNELIWREFFMQVLYHFPGVV
                     GSNFRKKFDSITWRNVPADFGRWQHGETGHLLVDAGMRELNTIGYMHNRVRMVVADYL
                     CKHLLIDWRWGEAYFATKMLDYELSSNNGNWQWAAGTGCDATPYFRKFNPTLQLSKFD
                     PQMNYVKQWIPEVRLLKE"
     gene            3989..4270
                     /locus_tag="PLM68_04705"
     CDS             3989..4270
                     /locus_tag="PLM68_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54697.1"
                     /translation="MNKEEFKARANQRIDEISARINELKAEKESVKGDLKKKYQESIK
                     ELETIKSDLQEKYDQMINAADDKWDEVRDAFSQAADSFREGWSKIKSAF"
     gene            4283..5188
                     /locus_tag="PLM68_04710"
     CDS             4283..5188
                     /locus_tag="PLM68_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT54698.1"
                     /translation="MAHEHAHNAPVAYARHFAIGIGLNILYVIAEVTFGLIANSSALL
                     ADAGHNTSDIMGLAFAWAAAWMATIRPKGKYTYGLRKTTILVSILNALLLFGAVALIA
                     WDAVEKLKNPQPVAGTSVMLVAGIGILVNSLTALLFYKGQKTDLNIKGAFLHMAADAA
                     VSLGVVIAGLLIHVTGRMWIDPAVSFLIILVILWGTWRLFAESLNLALDAVPRHINIE
                     KVRDFLLTREGVTSIHDLHVWAMSTTQVALTVHLVVPGGASDAFISRLQQELSDTFGI
                     GHTTFQVENKDLQVGHCNGGCPPNS"
     gene            5208..5753
                     /locus_tag="PLM68_04715"
     CDS             5208..5753
                     /locus_tag="PLM68_04715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT54699.1"
                     /translation="MIKLFLVSSLSLFLVSCGHQTREQVLFNSQKEPLEQRQPGQPES
                     LQHTQADQSLPQQGWVSCYRIPALATAPNGNLIAACDQRFSSCADPGCNAGLARVGER
                     LFFSNPDSPDRRENLTLRMSDDGGKTWSEGKTICAGSSAYSSLTVLLGGDVGVLYERN
                     DYKEIVFTRLKKSEIITNHKR"
     gene            5750..7471
                     /locus_tag="PLM68_04720"
     CDS             5750..7471
                     /locus_tag="PLM68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT54700.1"
                     /translation="MNKILPLLALLMACAACSTEQTPDTLCNPMDLSYRFALEDPFPS
                     RREAADPTMVRFGEKYLLFASKSGGYWYSDDLKEWDFIQTDQIPTEDYAPTTVAIGDT
                     LYFLASSNEKSTVYKSADPLSGTWEVAVEALEVPVWDPAFFLDDDRRLYLYWGCSDQR
                     PLYGVELDCRNGFAFLGDVRELVYANPAEYGWEVPGDYNTLTDRAPWIEGPWVTKQGG
                     KYYLQYAGPGTEYKSYADAVYLSGDPLGPFMLQKHNPAMCKPEGFATGAGHGSSFADG
                     YGNFWHIGTMTISQKHMFERRLGMFPVFLDEDGTYYAITKYGDYPFVVPRQKIQSFED
                     IFPGWMLLTYNKPVRVSSFVDSMPASYMTDEDIRTCWAAESGDAGEYAMVDMGDFFDV
                     YALQVNFAEHDVNIRGRQKAIYHRFTLEYSLDGNTWEMLSDRSVNNTDNTHVYIPLEE
                     KVLCRYLKITNIEVPGGHFAISGFRAFGKGAGPLPGRITSFTAQRNLQDKRSVSLSWS
                     KADHATGYNISYGTSGNKLYLDHMVYGDTTLVINNLNSNLDYYFTIESFNENGITPNQ
                     QIIRHFE"
     gene            7471..7965
                     /locus_tag="PLM68_04725"
     CDS             7471..7965
                     /locus_tag="PLM68_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HOT54701.1"
                     /translation="MDHFAAIDFETANEQPSSVCSVGIVIVRYGIPEQTIYRLIRPEP
                     DYYLYRNTMIHGLSAIDTDNARVFSHVWKEIEPFIQDLPLVAHNAQFDEGCLKAAFFT
                     YCMDYPDYEFFCTLQASRRHFGKLLPNHRLPTVAAACGHRLTSHHNALDDAIACAAIA
                     LKIL"
     gene            8025..9113
                     /locus_tag="PLM68_04730"
     CDS             8025..9113
                     /locus_tag="PLM68_04730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT54702.1"
                     /translation="MNKKPLLVVLFLAVMWSCTPDYYDQVFQIAREAGIPLIQVAYTK
                     GNTRVTYEVSTVPEIVARKDGKTVFQAASLSKPVFAYIVMRMSDRGEIDLDRPLFEYT
                     DIERFVDKELARRLTARMVLSHMTGLPNWAVSPGSPEWPEAPIVFKFRPDSAFSYSGE
                     GYAFLQRAVEQIQGKGLEEIARNEVFLPLGMSSSSYTWRDDYDSLAVNGFNRDGEDRG
                     KGNFPRANPAYTLRTTAADYMRFLEALSLGTGLGKESREAIFAPLVQAVRYEGKPREC
                     DQRIFWGLGVGIEKHPELGEVAFHWGDNGAFRSLFLVVPKRGLQPQRILIYFTNSQAG
                     HDIIHEISSLFLDNKEPIAIHEWVYKNE"
     gene            complement(9188..10363)
                     /locus_tag="PLM68_04735"
     CDS             complement(9188..10363)
                     /locus_tag="PLM68_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOT54703.1"
                     /translation="MNTGKYVFSQLIEFLPKRIFDGIVKRHSGDKWTKAMSCWNQLLL
                     MMFGQLSGCDSLRELACVIAAHQSKSYHLGFGKGPFARSTLEYANANRDYKIFEEFAY
                     YMINLAQSKRIDREFVINGKFYAFDSTTIDLCLSLYEWARFRKTKGGIKVHTLFDVVT
                     QIPVYIHITEAKIHDMNAMDDIPYEPYAFYIFDKGYYDLARLYTIATIGSHFIIRQKS
                     SFNYEVIDGEDVLDGADNVLLDQMIRPVGYHTKKKYPAELRRIVYYAPDLKRTFTFLT
                     NNRELKAKDIALLYKERWQVELFFRWIKLHLRVKSFWGNTENAVRIQIYTAIATYCLV
                     AIVEHDCQLNRSTFNVLRVLSHSLLDKTSIRDLFSNSESLDDRYIEDCSQLKFDFVY"
BASE COUNT         2865 a         2406 c         2603 g         2666 t
ORIGIN      
        1 gcggctaacc atagcactgg aagccgggaa tatggcctgg tgggaaatgg aactgacatt
       61 aggaaaaatt gttttcagtc ataacaaaac acgaatgttg gggctgaaag gtgaagattt
      121 taatcacttt caggatttca taaaaattgt gcatcccgat gatcgtgata ctaccatgaa
      181 tgccatgtat gaccacttgc ggagagatgc ggaaatgtat gaatgtgagt accggatgaa
      241 aaatgcggag ggcaattact aatggtttca cgatgccgga aaaatcgtat atcgggaagg
      301 ggagaaattg ctggttgccg gtattgttac tgaaatcact gaaagaaaac gcatagaagc
      361 acagctaaaa gaggcaaagc agcaggccga gattgccaac caatacaaga accagttcct
      421 ggcaaacgtg agtcacgaga tccgtacacc tacctgtaag cggcatagtg ggctttgcgt
      481 cgttgctgca ccagcaaaac ctggataccg aaaccagaaa tccatatatc gatatcattg
      541 aaagcagttc taggcaattg ctgaatttgg taaacgatat tattgatata tccagaatag
      601 aagcaggtga attgaaaatc aacaaaacac cctttgaatt acataagatc ctaaaagaga
      661 cggaaacctt gtttggaaaa ttaaaacatg cccgtggcaa acaaaacctg aaaatcaaac
      721 tggatattcc ggaaagggac caggtactgt acattgtaac cgatcccgac aggttaaagc
      781 aaatactaat caatctgata gggaatgcat tgaaattcac agaaaaaggc ggtattgatt
      841 ttggatacag aatagaggat gaccgcttga aattctacgt gtcggacaac ggaataggta
      901 tgacagaaga cgatctaagg ttggtttttg aacgtttcaa gcgttctgaa caggcacata
      961 ggaaatacga aggtactggt ttgggcctgg ctataaccaa agagcttctg gatctgctgg
     1021 gcggttccat atccgtggaa tcacaactgg gaagaggcag cgtatttacc ttccggatcc
     1081 catatgtacc tgcaaaatca caggaagcta cccacaacaa tgaacagaca acggatctgg
     1141 aacgtttacg tggcaaaaaa agtctgatag ccgaggatga aaaagtaaac aggatgtacc
     1201 ttgaaatcat cctgaaagaa ttgccaatag acatttactg ggccaagacg gccaacgctt
     1261 tgtccagtga ccgggaaaaa tacctggaaa gcggctttgt tgaccatata tccaaaccaa
     1321 ttgacaggct acagttgata cggatcatgg aaaggtgggc attgtaaagg acagatgaca
     1381 atacggccgt ggaaatgtga actccccatg cagtatactt ccgggaaatt catttagtgt
     1441 ataaacaatc tacttaccat gtcagtcata caccttttca aaaaggcagc cgttattgca
     1501 acagctcttg tttttgtctt cactttgcag ggatgtcctt acatagatcc ggatcatgat
     1561 tatgaatttg aatacgagat catcattaca gacgtacctg ccaacctgga aggtttcaat
     1621 tcgcagtaca acgattacaa ttcagcctta ccttttgaag ccaaccgccg cgagatttat
     1681 ttctctaccg acaggaaaac caacggcagt acatacgata tcataagcca tgcctttgac
     1741 atctcctacc acaaaaggga cgatgtcctg aacatctcca ttcctgtcaa tccgggagag
     1801 tccataatgg accggaaact gctgccgctt ataaacacag gctatgacga actgggtccc
     1861 tacacctatt tcgaagatgg aggtctgaac tatttctttt atgcaggcaa cggatctgat
     1921 gagcaagagg aacctggtga ttatgacata aaatttgtct acatgccacg ctcggactgg
     1981 acaacatacg gcgggcagca acgtctgttc ggtccctatg atgtcagttt ggccaattca
     2041 gaagcagacg acctgtatcc tgtcatatca gataagctgg gcaaaatgct tttttgcagc
     2101 aaccgggaga acgattcttt tgacatatac agccttgagc tggaacaggg agccctgctg
     2161 aaccagttcc tgacaggcaa ttatgagcca gtcattgcca aagagccggc cctttccgga
     2221 gagggccatg acaaatgtcc ttcaatcttt ggtgactatg tgatcttcgc ttcggacagg
     2281 gaaggcgggt acggcggatt cgatctgtat tatgcaagat atgagaataa cgcctggagc
     2341 gaaccggtga atttcgggga taagataaat acggccagcg acgagtacag gccgcttggc
     2401 tttaaattcg tggacttcag ccttatgatc ttttcttccg acaggcccgg tggcaaaggc
     2461 ggctttgacc tgtattgcgt ggatgtgagt gatatcattg aagttcaaga ttggtaccga
     2521 ttttatgagt tctgactata tttgaaggta ttttttgaga tacctgaaaa tatggaaaag
     2581 gtctcgtttt tttggtttcg cagggatata cgactggaag acaaccgcgg gctgcgggaa
     2641 gttctgcttg ggccatagag cagcactccg ttgcagcagc cggccccggc gccgacacgg
     2701 gctcccgtaa tgagggaacc tgcgtcggca aactgtttta caagaaagga actcccgggg
     2761 aagtctggat agcgctgctg gaacaattca acatcgtggc ggtttatgcc aatgaggact
     2821 acgaaccgta tgccattgca cgggacagtg agattgaagg attgctggct caaaaaggcg
     2881 tgctctttca cctttgtaaa gaccaggtga cctttgcccg tgacgaggtg gtcaagaaag
     2941 acagaacacc ttacaccctg ttcacacctt ataaaaatgc atggatcaaa aaaattgctg
     3001 aactgcacgt aaagccgata cagaacctgc ttgaggaccc actgcatgaa ccgctgccgg
     3061 cccgacttca aaccgggtta ccggcccgac ttcaaaccgt gttacctgcc cgacttcaaa
     3121 ccgggttacc ggcccgtttt gcggaatttt cttttccgtt cccatccctg actggaatag
     3181 gattcaaacg ttcttccctg aaagtacggg attacaatat agacaacctt gacaactacg
     3241 ataaagacag gaattatcct gccctggacg gtaccagcca tttttctgtt cacttgcgtt
     3301 tcgggaccgt gggcatccgg agcatcatga gccacataca acataatgaa agtttcctga
     3361 atgaattgat ctggcgggaa ttcttcatgc aggtcctgta ccattttccg ggtgtggtag
     3421 gctccaattt ccgcaagaaa ttcgattcca tcacctggcg caatgtccct gcggattttg
     3481 gaagatggca acatggagaa accggtcatc tcctggtaga tgcagggatg cgggaactga
     3541 ataccatcgg atacatgcac aacagggtcc ggatggtggt ggccgattat ttgtgcaagc
     3601 acctgctcat agactggcga tggggagaag catattttgc caccaaaatg ctggattatg
     3661 aactctcatc caataacggc aactggcaat gggccgccgg aacgggttgt gatgcaacgc
     3721 catacttcag gaagttcaat cccacattgc agcttagcaa attcgacccg caaatgaact
     3781 atgtgaaaca atggattccg gaggtacgac ttctaaaaga atagccctgt tcctgctgag
     3841 cagaacgctt cattatttgg ctcttctgtg gagatgctcc tggtgtattc gggcatcctg
     3901 cttctttgaa caggaataat tttctatgta agtagcagga agctgatatt tcgttaattt
     3961 tatgctcaaa tgcttaaata agactactat gaacaaggaa gaattcaagg cgcgcgccaa
     4021 tcaacggatt gatgaaattt cggccaggat caatgagctg aaagcagaaa aggaatcagt
     4081 caagggggat ctgaagaaaa agtaccagga atccatcaaa gagctggaaa ccataaaatc
     4141 agacctgcag gagaaatacg accagatgat caatgctgcc gatgacaaat gggatgaggt
     4201 ccgggatgct ttctcccagg cagcagattc gttcagggaa ggatggtcaa aaatcaaatc
     4261 ggcgttctga gcctgacacc gcatggcaca tgaacacgcc cacaatgccc cggtcgctta
     4321 tgccaggcat tttgccatag gcattggcct gaacattcta tatgtcattg ccgaggtgac
     4381 atttgggctg atagccaact catctgctct gttggccgat gcagggcaca ataccagcga
     4441 catcatgggc cttgcctttg cctgggcagc cgcctggatg gccaccatca ggccgaaagg
     4501 gaagtatact tacggattgc gtaagaccac catcctggtc tccatcctga atgcgctcct
     4561 gctttttggt gccgtagcgc tcatagcctg ggatgccgtt gaaaagctta aaaacccgca
     4621 acccgttgca ggaaccagcg tcatgctggt tgccggtatc ggtatcctgg tcaattcgct
     4681 tactgccttg ttgttttaca agggacagaa aaccgacctg aatatcaagg gcgctttttt
     4741 gcatatggcg gccgatgccg ccgtctctct gggagtagtg atagccggac tgctcatcca
     4801 tgtgacgggc aggatgtgga tagacccggc cgtcagcttt ctgatcatcc tggtgatttt
     4861 gtggggtacc tggcgcttgt ttgccgagtc gctgaacctg gccctggatg ccgtacccag
     4921 gcatataaac atagagaagg tacgtgattt cctcctaacc cgggaaggag tcaccagcat
     4981 tcacgacctg cacgtgtggg ccatgagcac cacccaggtg gccctgaccg tacacctggt
     5041 ggtacccggg ggagcctcgg atgcattcat cagccggttg cagcaggaac tgagcgatac
     5101 gttcggaata gggcacacca cgttccaggt tgaaaacaaa gacctgcaag tgggccattg
     5161 taacgggggc tgcccgccta actcctgaat ttcattatgt tgaaactgtg ataaaattat
     5221 tcctggtatc ttcactctcg ctattcctcg tttcatgtgg acatcagaca cgggagcagg
     5281 tgctttttaa ctctcagaag gaacccctgg agcaacgcca gccggggcag cctgagtcac
     5341 tgcagcatac gcaagcggac cagtcgcttc cacagcaggg atgggtttcg tgttacagga
     5401 taccggccct ggcgaccgct cctaatggga acctgatagc ggcctgtgac cagcgcttct
     5461 cctcctgtgc cgatccaggg tgcaacgccg gactggcacg ggtgggggaa cgactcttct
     5521 tttccaaccc cgacagccca gaccggaggg aaaacctgac gctccggatg agtgatgacg
     5581 ggggaaagac ctggagcgaa ggcaagacaa tctgtgccgg aagctcggcc tattcttcat
     5641 tgacggtgct tttgggaggg gatgttggag tgctttacga aagaaatgat tataaggaaa
     5701 ttgtatttac ccggttgaaa aaatctgaaa taattacgaa ccataaaaga tgaataagat
     5761 tttacccttg ttagccctgt tgatggcatg tgctgcctgt tccacggaac aaacccccga
     5821 tacgctgtgc aatcccatgg atctttctta ccgttttgcg ttggaggatc cttttccttc
     5881 acgcagggag gcggctgatc ctacgatggt ccggttcggg gaaaaatatt tgttatttgc
     5941 ctccaaatcg ggtggatact ggtactcaga cgatctcaag gaatgggact ttatccagac
     6001 tgaccagatc ccaacggaag attatgcccc tacaacggtt gccattgggg atacgctgta
     6061 tttcctggcg tcttccaacg agaaaagcac cgtgtataaa agtgccgatc cgctttcagg
     6121 tacctgggag gttgcggttg aagcactcga agttcccgtc tgggacccgg cctttttcct
     6181 ggatgatgac cggcggctat acctgtattg gggttgctcg gaccaaaggc ccctgtatgg
     6241 tgtggagctg gactgccgca acggatttgc ctttttgggt gatgtcaggg aactggtata
     6301 tgccaatccg gctgaatacg gatgggaggt gcctggggat tacaatacgc tgacggaccg
     6361 ggctccctgg atagagggtc cgtgggtaac taaacagggt ggaaaatatt acctgcaata
     6421 cgctggcccg ggcaccgaat ataagagtta tgccgatgct gtttacctct ccggggatcc
     6481 actgggtccc tttatgctgc aaaagcacaa tcccgccatg tgcaaaccgg aaggctttgc
     6541 aacaggggcc ggtcacggca gctcttttgc cgatggctat ggcaattttt ggcatatagg
     6601 cactatgaca atatcgcaaa agcatatgtt tgaacgccgc ctgggtatgt ttcccgtttt
     6661 cctggatgaa gacggaacgt attatgcaat aacaaaatac ggggattacc cgtttgttgt
     6721 gccccggcaa aagatccaaa gttttgagga catcttcccg ggatggatgc tgcttaccta
     6781 taacaaaccg gtgagggtgt cttcttttgt ggattctatg cccgccagct acatgaccga
     6841 tgaggatatc cgtacctgtt gggctgctga aagcggtgat gcgggcgaat atgccatggt
     6901 ggacatgggt gatttctttg atgtgtacgc ccttcaggtc aattttgcgg aacatgatgt
     6961 taacataaga ggaaggcaaa aagctattta tcaccgtttt actcttgaat attccctgga
     7021 cggcaataca tgggaaatgc tgtcggaccg ttccgtcaac aacaccgaca atacccatgt
     7081 gtatattcct ttggaagaaa aggtactgtg ccgatatttg aaaatcacca atattgaagt
     7141 tcccggcggg cattttgcaa tcagcggatt cagggctttc gggaaaggag ccggaccgct
     7201 gcccggcagg atcacgagtt ttactgccca aaggaatctt caagataaaa gaagtgtgtc
     7261 gctttcctgg tccaaagcgg atcatgcaac cggttacaat atcagttacg gtacatcagg
     7321 aaacaagctg tacctggatc acatggttta cggggacacg acattggtga tcaataacct
     7381 gaacagcaac ctggattatt atttcaccat tgaatctttc aatgaaaacg gcataacgcc
     7441 aaaccaacaa attatcaggc actttgaatg atggatcatt ttgcggccat tgattttgag
     7501 acagccaacg aacagccttc cagtgtatgc agcgtaggga tagtgattgt acggtacggg
     7561 attccggagc agaccattta ccggcttatc aggccagaac cggattatta cctgtaccgg
     7621 aacaccatga ttcacggact cagtgcaatt gacacagata acgcacgcgt gttttcacat
     7681 gtgtggaagg aaatagaacc tttcatacag gatttgcccc tggtggccca taatgcccaa
     7741 tttgacgagg gctgtctgaa agcggcattc ttcacctatt gcatggatta tcccgattac
     7801 gaattttttt gcacccttca ggcgtcacgc aggcactttg gaaaattatt gccaaatcac
     7861 cggttaccaa ccgttgcagc tgcctgcggg catagactga ccagccacca taacgcgctg
     7921 gacgatgcca ttgcctgtgc tgccattgcc ctgaagatat tgtaaaattt tattttttat
     7981 tacctttgct taatactccg ctgatgttga cagccggaaa atgtatgaat aaaaagccat
     8041 tactggttgt cctttttctt gcagtgatgt ggtcctgcac acccgattat tatgaccagg
     8101 ttttccagat tgcccgtgaa gcggggattc ctctcataca ggtggcctat acaaagggga
     8161 atacccgggt tacgtacgaa gtatccacgg ttccggagat agtggcacga aaggacggta
     8221 agacggtttt ccaggctgcc tcgctcagta aacccgtgtt tgcctacatt gtcatgagga
     8281 tgagcgacag gggagagatt gacctggaca ggccgctttt tgagtatact gacattgaac
     8341 gtttcgtgga taaggaactg gcccgcaggc ttacggcccg gatggtcctt tctcatatga
     8401 ccggcctgcc caactgggcg gtttcacccg gttccccaga atggccggag gcgcccattg
     8461 tttttaaatt cagacccgat tcggcattca gttactctgg agaagggtat gcttttttgc
     8521 aaagggcggt ggaacagatc caggggaagg gactggagga gattgcccgg aacgaggtgt
     8581 tcctccctct gggaatgagc tcctcgtcct atacctggag ggacgattat gactccctgg
     8641 ctgtgaacgg ttttaatcgt gatggcgaag acaggggaaa ggggaatttt cccagggcaa
     8701 atcccgccta taccctaagg acaacagcgg cagattacat gaggtttctg gaggcgttga
     8761 gcctgggcac cggcctcggc aaggaatccc gggaagccat atttgctccc ctggttcagg
     8821 cagtcaggta cgaaggaaag cccagggagt gcgaccagag gatattctgg ggccttgggg
     8881 tgggaataga aaagcatcct gaactgggcg aggtggcatt tcactggggg gacaacgggg
     8941 ctttcaggtc gctgttcctg gtagttccca aaaggggatt gcaaccacaa agaattttga
     9001 tatattttac caatagccag gcaggccatg acattataca tgagatatct tctcttttcc
     9061 ttgataacaa agagccgata gctattcatg aatgggtgta taaaaacgaa taaactgatt
     9121 actttatgaa atctgaaaga ttatctgact tcactagtgg ccaaataaat tatggcctat
     9181 ttttaagtta ataaacaaag tcaaatttca gttgggagca gtcttctata tatctatcgt
     9241 caagggattc agagttcgaa aaaagatctc tgatagatgt cttgtcaagc agcgagtgac
     9301 tcaacacacg gaggacgttg aatgtactcc tattaagttg gcagtcatgc tcgacaatgg
     9361 ccacgagaca atatgtagca attgctgtat atatctggat tctaacggca ttctcagtat
     9421 ttccccaaaa agattttacc ctgagatgca attttatcca acgaaaaaac agttcgacct
     9481 gccatctttc cttataaagc aaggcaatgt ctttggcctt gagttcgcgg ttgttggtca
     9541 ggaacgtaaa cgttctctta aggtcaggag catagtacac tattctccgc agttcagcag
     9601 gatacttctt tttagtatga tagcccacag gtcgtatcat ctgatccaga agcacattat
     9661 ctgcgccgtc cagaacatcc tcgccatcaa tcacctcgta attaaagctg gacttctgtc
     9721 gtatgataaa atgcgaacct attgttgcaa ttgtgtacaa cctggcaagg tcataatacc
     9781 ctttatcaaa aatataaaac gcatatggtt cataagggat atcatccatt gcattcatgt
     9841 cgtgtatttt agcttctgtg atatgtatat aaacaggtat ctgagtgact acatcaaaca
     9901 acgtatgcac cttgataccc cctttggttt ttctaaagcg cgcccattca taaagactca
     9961 ggcagagatc gattgttgtg gagtcaaatg cgtaaaattt cccatttata acgaactccc
    10021 tgtcaattcg cttgctctgc gcaaggttta tcatgtaata ggcaaactcc tcaaaaattt
    10081 tgtagtctcg gtttgcgttg gcgtattcca gggtacttct ggcaaacggt cctttaccaa
    10141 atccgagatg gtaactctta ctttgatggg cagcaatgac acaggcaagt tctctgaggc
    10201 tgtcgcaacc agacagttgt ccaaacatca tcaaaagcaa ttggttccaa caggacatcg
    10261 cttttgtcca cttgtcccca ctgtgccgct tcactattcc atcaaatatt cttttaggca
    10321 aaaactcgat aagctgactg aatacgtatt ttcctgtgtt catgcttgcc tgggttataa
    10381 tcaggctgta atgatacgaa atcaattcaa atcggcaccg aatatttttg cttataatta
    10441 attatatatt aacaatttat aacgaccaat caaaaattta tttggccact agtgatctga
    10501 cttaataatg atgtctccct ttgcctttgc ttctcaccag
//
LOCUS       DAOSOF010000060        10423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6395_length_10423_cov_4.511285, whole genome shotgun sequence.
ACCESSION   DAOSOF010000060 DAOSOF010000000
VERSION     DAOSOF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6395_length_10423_cov_4.511285"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLM68_04740"
     CDS             complement(<1..1221)
                     /locus_tag="PLM68_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOT54704.1"
                     /translation="MFTEQDIQTIERKGLTLKDAETHLHYFSAGFPPLDISGPAVPGN
                     GIVQLDGQEQEEMIKRYDEWNGSRIKFVPASGAASRMFKDLFEARDLLKKDRNALMPD
                     QLNDFFGRLPEFAFYPILSGLKGFDPKDRYGILSLILDRDGLNYASLPKGLIPFHKYS
                     NGPRTPFEEHLVEAALSSAQPDGTVRLHFTVSEEHLHLFNDLWEKVQKVYEELFQTSF
                     IVGFSTQSPSTDTLAADIENRPFRDQNGLLVFRPGGHGALLDNLNTLKEDMVIIRNID
                     NVVPQAALGPVIHWRKVLAGYLLFCRRKIYKYIGELKNHADPICMKEIALFLESNFGI
                     THPPMEGEEFRSYLFSKLNRPVRVCGMVPATGEPGGGPFRVVDRDGSGSLQILESVQL
                     QGKRYASTHFNPVDI"
     gene            complement(1191..2249)
                     /locus_tag="PLM68_04745"
     CDS             complement(1191..2249)
                     /locus_tag="PLM68_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOT54705.1"
                     /translation="MDARPHIIADEQVPFLEGITEKHARITRMPGNLIDKQAIRNANA
                     LLVRTRTVCSASLLENSRISFIGSPTIGTDHIDMDYCASAGIVVANAPGCNAEAVMQY
                     VFTALVAWAGSHICDLRNKTLGIVGVGNVGKKVAALASHLGMRTILNDPPRAEAEGPE
                     GFSDIGFLLEQSDIVTMHVPLTELTLNMADNHFFSSMKKGACFINTSRGAVADEKALY
                     RAAPNLGGIILDVWQGEPHINPATLQVSNIGTPHIAGYSLEGKRNASEQVVRALAGHF
                     GWKELESYTVPLPDASHVPADLLFTQCFPIFEQDKQLRNAPEKFEQIRSAYVFRREWT
                     SEQYRQLTLCLPNKTSRQ"
     gene            complement(2252..2542)
                     /locus_tag="PLM68_04750"
     CDS             complement(2252..2542)
                     /locus_tag="PLM68_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOT54706.1"
                     /translation="MKRQQTRTLNEILEQILNETELANGLEILRINHAWNELIGETAA
                     RECGDHTFREGVYSVRVKSSVLRCQLDMQKVFLAGKLNAMLKKDLVREIVIR"
     gene            complement(2502..3629)
                     /locus_tag="PLM68_04755"
     CDS             complement(2502..3629)
                     /locus_tag="PLM68_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOT54707.1"
                     /translation="MHLSRLLLSHFKNIDNAELEFSPGINVLYGDNGEGKTNLLDGIY
                     YLSVCKSSLSLQDKDLIDHASREAALYGEYVVDSGMTEKISCSITRQGEKIFKRNGKR
                     YEKLSLHVGLIPVVMVSPQDTSLVFGPAEERRKYMNFLLSQTGPGYLQTIQEYNKLLF
                     QRNRLLKSEHRPTDVLDALDASLSSRGDRIHDHRMELCKLLHPHVIHYYELLSAGKEQ
                     VGLSYESDLNQESMAVQLRKNRQKDMILQYTGTGVQRDDIHFTLGDHPLKVCGSQGQQ
                     KTFLLALKLAQFALISQMKELAPVLLLDDVFDKLDARRVAHLLQLVTKNGFGQIFLTD
                     SNKVRIEEIIHALGGPHSIYRVKGGVFTNEKTADQNTERNS"
     gene            3701..4399
                     /locus_tag="PLM68_04760"
     CDS             3701..4399
                     /locus_tag="PLM68_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54708.1"
                     /translation="MSKKKTSAKSPQKKEEQAKVAQAISKSEEFLNKYKKHIIYTVAG
                     IVILLALVFGYMKLIREPRRNEALAQLFPAEQYFRVDSFYLALNGDGNVLGFKDIIDE
                     YKSITGQAVYFYAGVCELQLGNYEQAIDYLKKYKAGDDIIQARAWCNIADAYAGLDEL
                     DNAISWYMKAAGYSDNAYAATYYKKAGILMEEKSNYSGALEMYKTIKDKYPQTIEAYD
                     IDKYISRLEILMQQ"
     gene            4409..5128
                     /locus_tag="PLM68_04765"
     CDS             4409..5128
                     /locus_tag="PLM68_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT54709.1"
                     /translation="MGRAFEYRKARKLKRWGNMSRTFTRLGKELTIAAKEGGTDPENN
                     PRLRAIIQTARSENMPKENIERAIKRAEEKDKGDYREVVYEGFGPHGVAIIVETATDN
                     TTRTVANIRSYFNKFGGNLGTSGSVDFMFDHISVFKVRADQVTDMETFELELIDYGGE
                     EIFQDEEEGVIVVYGSFESYGSLQKFIEEKGLELVSGGFDRIVNGEMKQLTEEEKADV
                     EKLLERIEEDDDVQNVYTNMA"
     gene            complement(5125..6993)
                     /locus_tag="PLM68_04770"
     CDS             complement(5125..6993)
                     /locus_tag="PLM68_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOT54710.1"
                     /translation="MKSPYKYKNHTGIKYLFTLAGRLSIVLILLLLSRLAFYLYNGYL
                     FPETGWETMPLVYRGGIRFDLSALFYMNGLYLLLALLPLPFVFSRHYQVFLKIFYLLV
                     NAAGFFFQLFDFVYFRTTLRRIDFSFFREFANERNLGMVLLKALKDYPAFFLIWAGVI
                     FLLWFSYGRSNFPLRPPFTIRFFSARLVILLLAAVITVVAIRGGTDRTTRPITLGNAA
                     AYVTRPLEMGLVLNTPFCIMRTFGKPSVERLDFFPSEEELNRVYSPEHRRDTISPDVR
                     EPEPAMNVVILILESFSREYSGYLNPGASTSYMPFLDSLMAHSLVCSKAYANGRKSVD
                     AVPSVLGGIPSLEQSFALTPFALNRVEGLGNALKKRGYNTAFFHGAPNGSLGLDGMSR
                     HFGFDSYYGMKEFADRSLFDGYWGIWDEPFLQFFAEKLQTFEEPFAAVVFTLSSHHPF
                     IVPEEYEGFFPESSLPLHQCIQYSDNALRLFFEKARLSSWYQNTLFVLVADHGAYSQV
                     NPSYQSEVESMAIPVIYHDPSGIVVQGGQIYDQYTQQIDIMPTVLDMLDYPHSFFAFG
                     RSIRDSLITPFVVNYPALFNIMRQEKEKEPSNELFLKAFRQQYNNRLLDDKLYVGD"
     gene            7112..7522
                     /gene="mce"
                     /locus_tag="PLM68_04775"
     CDS             7112..7522
                     /gene="mce"
                     /locus_tag="PLM68_04775"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOT54711.1"
                     /translation="MNIKHIEHIGIAVRSLEQSIAFYETKLGLPCYAIEEVPEQKVRT
                     AFFKVGQTKVELLESTDPEGPIGKFIENRGEGVHHIAFATEGLQENLDNLAANGIRLI
                     DKSPRKGAEGLHIAFLHPKDTLGVLTELCENPKK"
     gene            7538..9091
                     /locus_tag="PLM68_04780"
     CDS             7538..9091
                     /locus_tag="PLM68_04780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT54712.1"
                     /translation="MSNQLEQIRELIQLREKARLGGGQKRIDAQHEKGKCTARERITM
                     LLDEGSFEEFDMFVTHRSSNFGMQDKTILGDGVVTGYGTIEGRLVYVFAQDFTVFGGS
                     LSETMSMKICKIMQQALKVGAPLIGINDSGGARIQEGVNALAGYGDIFQNNIIASGVI
                     PQISALFGPCAGGAVYSPALTDFTIMMRGSSYMFLTGPKVVKTVTGEDVTQEELGGAT
                     VHASKSGVAHFAVDTEEDGVELIRDLLSYLPQNNMEEPPQVECLDPIDRLDDSLNDII
                     PDSPNKPYNMYEIIHSLVDDGKFLEVHKDYATNIIVGFARFNGTSVGVVANQPRVLAG
                     VLDINASRKAARFVRFCDAFNIPLVTLVDVPGFLPGTQQEYGGVILHGAKLLYAYGEA
                     TVPKITVILRKAYGGAYIVMSSKHLGGDLNYAWPSAEIAVMGPSGAIEVLFGKELAEA
                     ENPVAFVEEKEKEYRDKFANPYTAAHYGYIDDIIEPRNTRFRIIRSLQLLATKKQSLP
                     PKKHDNLPL"
     gene            9103..10020
                     /locus_tag="PLM68_04785"
     CDS             9103..10020
                     /locus_tag="PLM68_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOT54713.1"
                     /translation="MKQSVKYILIGLLLFFRVSLFGQTVIDLRFNELLITNTEDFQDD
                     FGVHSSWFEIFNIGYGAVDIGGCYLSNDPNDLKKYPVPRGDVLTKIKPRQHILFWADN
                     KPYRGSFHVNFSLEDSKVLILTNADGKTILDMVTLPTNMEPNQSYGRVIDGEGSYGPG
                     SDNTGWDVLARTSPSTNNYTLDGKSRAAKMKETDPYGWIMALLAMSVVFLSLLILTFV
                     FKGTGRTSIRLSQKKADAYQKTKNFSVEDISGETFAAIAMALHLYANETEVHDIETTI
                     LTIEQVRKNYSPWSSKYQFMRKMPETRKR"
     gene            10041..>10423
                     /locus_tag="PLM68_04790"
     CDS             10041..>10423
                     /locus_tag="PLM68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT54714.1"
                     /translation="MKEYNLIINGNNYSVVINELEETLAEVEVNGTPYQVTIDKPLKK
                     SAPVQLVRAPRAAAPSAAKTSPVSGKAPSAGGSYTVNSPLPGVILEVSVKEGDTVKSG
                     QKLIVLEAMKMENNIEADRDGVVAS"
BASE COUNT         2917 a         2420 c         2502 g         2584 t
ORIGIN      
        1 gatatctacc ggattaaaat gcgtggaagc atatcgtttg ccctgcaatt gaacgctctc
       61 cagtatctga agcgaaccgc tcccgtctcg gtccactaca cggaaaggtc cccctcccgg
      121 ttctcccgta gccgggacca ttccacatac cctgaccggc ctgttcagtt tagagaacaa
      181 ataggaccgg aattcctccc cttccatggg aggatgagtg atgccaaagt tactttccag
      241 gaagagcgca atctctttca tgcagatagg atctgcgtgg ttcttcagtt cacctatata
      301 tttatatatc tttctcctgc agaacagcag gtagccggca agcacctttc tccagtggat
      361 cacagggccc agcgccgcct gcggtactac attgtcaata ttgcgaataa taaccatgtc
      421 ctccttcagt gtattcagat tgtccaaaag ggcaccatga cctccgggac ggaaaaccag
      481 caatccgttc tggtcccgga aaggtcggtt ctcgatatct gcagcgaggg tgtcggtaga
      541 aggactctgt gttgaaaaac caacaataaa gctggtttga aacagctcct catatacctt
      601 ttgcaccttt tcccataaat cgttgaacaa atgcaagtgt tcttcagaaa ccgtgaaatg
      661 gagccttact gtcccatcgg gctgagccga actcagtgct gcctccacca ggtgttcttc
      721 aaaaggagtc ctcggaccat ttgaatactt gtgaaaaggg atcaatccct tgggcaggga
      781 tgcataattc agaccatcac ggtccaggat gagcgagaga atcccgtaac gatccttagg
      841 atcaaatcct ttgaggcctg aaagaatggg ataaaaagca aattcgggga gtcttccgaa
      901 aaaatcgttc aactggtcag gcattagggc attcctgtct tttttcagaa ggtcccttgc
      961 ctcgaaaagg tccttgaaca tgcggctggc cgcaccggat gccggaacaa atttgatcct
     1021 tgatccgttc cactcgtcgt accttttgat catttcctcc tgttcctggc catccagctg
     1081 tacgattccg tttccgggaa cggcaggccc ggaaatatcc aggggaggaa aaccggcgga
     1141 aaaataatgt agatgagttt ctgcgtcttt aagggtcagt cccttacgct ctattgtctg
     1201 gatgtcttgt tcggtaaaca taaagtcagt tgcctgtatt gttcactggt ccactctctt
     1261 cgaaaaacat atgcgctcct gatttgttca aacttttcgg gtgcgtttcg tagctgttta
     1321 tcttgttcaa atataggaaa acattgggta aaaagcaaat cagcaggaac atggcttgcg
     1381 tcgggcaacg gaacggtgta ggactccagt tctttccatc caaaatgacc ggccagggca
     1441 cggactacct gctcagatgc atttctcttt ccttccagcg aataacccgc aatgtgggga
     1501 gtgccaatgt tactcacctg caatgtggca ggattgatgt gtggttctcc ctgccagaca
     1561 tcaaggatga tccctcccag gttcggtgca gccctgtaca gggctttctc atctgccacc
     1621 gccccacggg aagtattgat aaaacaagcc cctttcttca tagacgagaa aaaatgattg
     1681 tcagccatgt tgagggtaag ctcagtgagc ggaacgtgca tggtcacaat atcggactgt
     1741 tcaagcagaa atccgatatc ggaaaatcct tcaggtcctt cggcttctgc cctgggcggg
     1801 tcattcagga tggtgcgcat tcccaggtgc gatgccaggg ctgccacttt tttaccaaca
     1861 ttacccaccc ccacgattcc cagcgttttg ttcctcaaat cacatatatg gcttcctgcc
     1921 catgccacaa gcgccgtgaa aacatattgc attaccgcct ctgcattgca tccgggggca
     1981 ttagccacta cgataccggc agaggcacaa taatccatgt caatatgatc ggttcctatg
     2041 gttggtgacc cgatgaaaga gatcctgctg ttctctagca ggctggctga acagacagtc
     2101 cgtgtcctga ccagaagtgc gttggcattg cggatggcct gcttatctat caggtttccg
     2161 ggcatcctgg tgatacgggc atgcttttct gttatgcctt ccagaaaagg cacctgttca
     2221 tctgctatga tatgaggtct tgcgtccacg gtcaacgaat gacaatttcc cggacaaggt
     2281 ccttcttcag catggcgttg agtttgcctg caagaaaaac tttttgcata tccagctggc
     2341 aacgcaggac ggaagattta acccggactg aataaacccc ttcccggaaa gtatggtcac
     2401 cacattccct ggctgctgtc tctccgataa gctcattcca ggcatgattg atccttagta
     2461 tttccaggcc atttgccaat tccgtctcgt tcaggatctg ttcaagaatt tcgttcagtg
     2521 ttctggtctg ctgtcttttc attggtaaaa actcctcctt ttacacgata tatggaatgg
     2581 ggcccgccca aagcatgaat gatttcttcg atccgtactt tattgctatc tgtcaggaaa
     2641 atttgtccaa aaccattttt tgtgactagt tgcagaagat gcgctaccct tcgggcatcc
     2701 agcttgtcaa agacatcgtc aagcagcagg acgggtgcca attccttcat ttggcttata
     2761 agtgcgaact gggccagctt aagtgccagc aggaacgtct tttgctgtcc ctgtgagccg
     2821 cacaccttaa gaggatggtc tcctaaggta aaatggatat catcacgctg aacacctgtg
     2881 ccggtatact gcaggatcat gtctttctgc ctgttctttc ttaactgtac agccatgctt
     2941 tcctggttca gatccgattc atagctcaga ccaacctgct cttttcctgc agatagaagc
     3001 tcgtaataat gaatcacatg gggatgcagc agcttgcaga gttccatgcg gtgatcgtga
     3061 atcctgtcac ctctcgatga taaggatgca tccaatgcat ccagtacatc tgtaggtctg
     3121 tgttccgact tgagcagccg gtttctctgg aaaagcagtt tgttgtattc ctgtatggtt
     3181 tgcagataac ccggccccgt ctgggatagc aggaagttca tgtacttccg tcgttcttcg
     3241 gccgggccga aaacaagtga ggtgtcctgc ggggaaacca taaccacagg aatcaaacct
     3301 acatggaggg aaagcttctc atagcgtttc ccgttccttt tgaatatttt ttctccctgt
     3361 ctggtaatgg aacaggagat cttttctgtc atgccggaat ccacgacata ttcaccataa
     3421 agtgcagcct cacggcttgc atggtctatc aggtctttat cctgtaaaga aagcgagctt
     3481 ttgcagacac tcaggtaata gatcccgtcc agcaggttgg tttttccttc tccgttatcg
     3541 ccgtagagaa catttatccc gggagagaat tccagttcag cattgtcaat gtttttaaaa
     3601 tgagatagca acaaacgcga taaatgcata cagattcctt caacaagtat gcaaacatag
     3661 caaattttac gtatatttgc ggccttaaaa acatagacct atgtcaaaaa agaaaaccag
     3721 cgcaaagtct ccgcagaaaa aagaagaaca ggcaaaagtc gcacaagcta tcagcaaatc
     3781 agaagaattt ctgaacaagt ataagaaaca cattatttat acagttgccg gaattgtaat
     3841 cctgctcgct ctcgtattcg gttacatgaa actgataagg gagccgcgcc gcaatgaagc
     3901 cctggctcag ctgttccctg ccgagcagta cttccgggtt gattcgttct acctggcact
     3961 gaacggagat ggaaatgttc tcggctttaa agatatcatt gatgagtaca aatccatcac
     4021 gggtcaggcg gtttacttct atgccggagt ttgcgagctg caactgggta attacgaaca
     4081 ggcaatagac taccttaaga aatataaagc cggcgacgat atcattcagg cgcgtgcctg
     4141 gtgtaatata gcagatgcct atgcagggct tgatgaactg gacaatgcca tttcctggta
     4201 tatgaaggct gccgggtata gtgataatgc ttacgcagcc acctattaca aaaaagcagg
     4261 catcctgatg gaagagaaaa gcaactacag cggtgccctg gaaatgtaca aaaccattaa
     4321 agacaaatat cctcaaacca ttgaggcata tgatattgac aaatatatat cgcgtctgga
     4381 aatactgatg cagcaataat ttcttgctat gggaagggct tttgaataca ggaaggccag
     4441 aaagctgaaa cgttggggga atatgtccag gactttcacc cggctgggca aagaactgac
     4501 cattgccgcc aaagaaggtg gtacagatcc ggaaaataat ccacggcttc gtgcgataat
     4561 acagacagcc cgttccgaaa atatgcccaa ggagaatatt gaaagggcta taaaaagagc
     4621 ggaagagaaa gacaagggag attaccgcga ggttgtctac gaaggatttg gtccgcatgg
     4681 tgttgccatc atagtcgaaa cggctaccga caataccaca aggactgtag caaatatccg
     4741 ttcttatttc aataaattcg gaggcaacct gggtacgtcc gggagcgtgg atttcatgtt
     4801 tgaccacata agtgtcttta aggtgagggc agatcaggtt acagacatgg aaacattcga
     4861 acttgagctg atcgattatg gcggagaaga aattttccag gatgaagaag aaggagtcat
     4921 tgttgtatac ggtagttttg aatcttatgg ctcgcttcag aaattcatag aggaaaaggg
     4981 gctggaactg gtaagcggcg gttttgaccg gatagtcaat ggtgaaatga aacaactgac
     5041 cgaggaagaa aaagcagatg ttgaaaaact gttggaacgg atagaagaag atgacgacgt
     5101 acagaatgtc tacaccaaca tggcctaatc cccgacgtac aatttgtcat caagcagcct
     5161 gttattgtac tgctgacgaa atgctttaag aaagagctca ttggagggct ctttttcttt
     5221 ttcctgcctc atgatgttaa aaagtgccgg ataattcact acaaaggggg ttatgagtga
     5281 atcccttata cttcttccaa aagcaaaaaa actatgggga tagtccagca tgtccagcac
     5341 agtgggcatg atgtctattt gttgtgtgta ttgatcgtag atctgtccgc cttgcactac
     5401 aatgcctgat gggtcatgat atatgacagg gattgccatg ctttccactt cggactggta
     5461 actgggattc acctgtgagt aggccccatg gtctgcaacc agcacaaaaa gggtgttctg
     5521 ataccagctg cttaggcggg ccttctcaaa gaagagcctt agtgcattgt ccgagtattg
     5581 tatgcattgg tgaagtggca ggctgctttc cggaaaaaat ccttcatatt cctcaggtac
     5641 aataaaagga tggtgggatg acaaggtgaa gacaacggct gcaaaaggtt cctcaaaagt
     5701 ttggagtttc tctgcaaaaa attgcagaaa gggttcatcc catatacccc agtagccgtc
     5761 aaacagggat ctgtcggcaa attctttcat cccgtaataa ctgtcgaatc caaaatggcg
     5821 ggacatcccg tccaggccga gtgatccgtt aggggctccg tggaaaaaag cagtgttgta
     5881 ccctcgtttt ttaagcgcat tgcccagccc ttccacccgg tttagtgcaa agggggtcag
     5941 agcaaatgat tgttccaggc tgggtatgcc tcccagcaca gagggaacgg catcgactga
     6001 ttttcgtccg tttgcgtaag ctttggaaca gaccagtgaa tgagccatca acgaatccag
     6061 gaaaggcata taagacgtgg aagcgccagg atttaggtac ccggaatact ccctggaaaa
     6121 actttccagt atcaggatca ccacattcat ggccggttcg ggttcccgga catccggaga
     6181 tattgtatcc cttctgtgtt cgggcgagta cacgcggttc agttcctctt ccgagggaaa
     6241 gaaatcaagc ctttccacag atggtttccc aaaagtgcgc atgatgcaaa acggagtatt
     6301 cagaaccaga cccatttcca gtggtcgtgt aacataggcg gctgcattgc ccagggtaat
     6361 gggccttgtt gtccggtctg ttcctccccg gatcgccacc accgtgataa cggctgccag
     6421 gagcaggatc accagcctgg cggagaaaaa gcgaatggta aatggtggtc ggagcgggaa
     6481 gtttgatctt ccgtaagaaa accataacag aaaaattact cctgcccata tcagaaaaaa
     6541 ggcaggataa tctttcaggg ccttaagaag caccatgcca aggtttcgtt cgttggcaaa
     6601 ttccctgaag aaagaaaaat caatcctgcg gagtgtcgtg cggaaataga caaaatcaaa
     6661 tagctggaaa aagaaaccag ccgcgtttac cagcagataa aagatcttta gaaaaacctg
     6721 gtaatgtctg gaaaaaacaa aaggaagggg caacaatgca agaagcaggt ataaaccatt
     6781 catatagaac agggctgaaa gatcaaaacg gatccctccc cggtatacca gtggcatggt
     6841 ctcccagcct gtttcaggga acaggtatcc gttatacagg taaaaggcca acctgctcaa
     6901 cagcaacagt ataagcacaa tggaaagcct gcctgcaaga gtaaataagt atttaatacc
     6961 ggtatgattt ttgtacttgt aagggctttt catagatagc cacaaaaata aataaactgt
     7021 cctaataatg ttcaaattgt tattcattag agataaattt cgtaattttg tggattaatt
     7081 ttaaaacctg cgtatcataa tccagcaatc tatgaatatt aaacacatag aacatattgg
     7141 cattgctgtt cgctctctgg aacaatccat tgccttttat gaaacaaaac ttggcctgcc
     7201 atgttatgcc attgaagaag ttccggaaca aaaagtgaga acggcatttt ttaaagttgg
     7261 tcagaccaag gtggaattac tggagagtac agatcctgaa ggtcccatag gaaaattcat
     7321 tgaaaacagg ggtgaagggg ttcatcacat agcttttgcc acagaggggc tccaggagaa
     7381 ccttgacaac ctggcagcaa acggtatccg cctgatcgat aaaagtccgc gcaagggggc
     7441 agaaggcctg catatcgctt ttctgcatcc gaaggatacc ctgggtgtgc taaccgaatt
     7501 gtgtgaaaat ccgaaaaaat aaacaattaa taataaaatg agcaatcagt tggaacagat
     7561 acgtgagttg attcaactcc gtgaaaaagc ccgcctggga gggggtcaga aacgtataga
     7621 tgcacaacac gaaaaaggta aatgtacggc tcgtgagcgt ataacgatgc tccttgacga
     7681 aggcagcttt gaagaatttg acatgtttgt gacgcaccga agcagtaatt tcgggatgca
     7741 ggataaaacc atcctgggag acggggtcgt tacaggttac ggcaccattg aagggcgtct
     7801 tgtttatgtt tttgcacagg atttcacggt gtttggcggt tcactttcag agaccatgtc
     7861 catgaagatc tgcaagatca tgcagcaggc cttgaaagtg ggagcccccc ttataggaat
     7921 aaacgactca ggcggagccc gtatccagga aggcgtgaat gcgcttgccg gttatggcga
     7981 tatcttccag aacaacatca ttgcgtccgg cgttatcccg caaatttcgg ccctatttgg
     8041 tccctgtgcc ggcggggctg tttactcccc tgcgctgacg gacttcacca tcatgatgag
     8101 gggttccagt tacatgttcc tgaccggccc aaaagtggtc aagaccgtga ccggagaaga
     8161 tgtgacacag gaagaattgg gaggcgcaac ggtccatgcc agcaagagcg gtgtagccca
     8221 ttttgcggta gacactgaag aggacggtgt ggaactgata cgcgatctgc tcagctactt
     8281 gccccagaac aatatggaag aacctcccca agtagagtgc ctggatccga ttgaccgtct
     8341 ggacgattct ttgaacgata tcattcccga cagtcccaac aagccctata acatgtacga
     8401 gatcatccac agcctggttg atgacggaaa attcctggaa gtccataagg attatgccac
     8461 caacatcata gtcggctttg cccgtttcaa cggcacttct gtcggtgtgg ttgccaacca
     8521 gcccagggtt ctggccggtg tgctggacat caatgcatca aggaaggccg ccaggttcgt
     8581 gcgtttctgt gatgctttca acattcccct ggtaactttg gtagatgttc cgggatttct
     8641 gcccggcaca caacaggaat acggcggagt gatcctgcat ggggcaaaac tgctgtatgc
     8701 atacggagaa gccactgttc ccaaaataac tgtaatcctt aggaaagcat atggcggagc
     8761 atacattgtg atgagttcaa aacacctggg aggagacctt aactatgcat ggccttcggc
     8821 agaaattgcc gtcatgggac cttccggtgc cattgaggtc ctctttggaa aagagctggc
     8881 cgaagcagaa aatcctgtcg ctttcgtgga agaaaaagag aaggaatatc gtgataaatt
     8941 tgccaacccc tacacagcag cacactacgg ctacattgac gatatcattg aacccaggaa
     9001 tactcgtttc aggatcatcc gctccctgca actacttgcc accaagaaac agagcctgcc
     9061 tcctaaaaag cacgacaacc ttcctttata aaccggacct ttatgaaaca atcagttaaa
     9121 tacatactca tcgggttgtt actgttcttc cgggtatcgc tcttcggaca gactgtgata
     9181 gacctgcggt tcaacgagtt gctgataacc aacacagagg attttcagga tgatttcggg
     9241 gttcacagca gctggttcga aattttcaat attggatatg gagcggtgga tattggagga
     9301 tgttatctga gcaacgaccc caatgacctg aaaaaatacc ccgttccccg tggggacgtg
     9361 ctgacaaaga tcaaaccaag acagcatatt ctcttttggg ctgacaacaa accttacagg
     9421 ggttctttcc atgtgaattt ttccctggag gattccaagg ttctcatact caccaacgca
     9481 gacggcaaaa ccatactgga tatggtgacc ttgcccacga acatggaacc gaatcaatca
     9541 tacggcaggg taatagacgg ggaaggatcc tatggcccgg gatccgataa tacaggctgg
     9601 gatgtcctgg caagaacctc acccagcacc aataattata cgctggatgg taaatcccgt
     9661 gctgcaaaga tgaaggaaac cgatccctat ggctggatca tggcacttct ggccatgtcg
     9721 gtggtctttt tatcgctcct gatattaact tttgttttca agggtaccgg aagaacctcc
     9781 atacggttgt ctcagaaaaa agctgatgca tatcagaaaa ccaaaaactt ttcggtggaa
     9841 gatatttcgg gagaaacttt cgcagccatt gctatggcct tgcatctcta tgccaacgag
     9901 actgaagtac acgacattga aaccacaata cttaccattg agcaggtaag aaagaattac
     9961 tctccctgga gttccaaata ccagttcatg agaaagatgc ctgagacgag aaaaagataa
    10021 aactgataat acaacgcact atgaaagaat ataatttgat aatcaacggg aacaattaca
    10081 gtgtggtaat caacgagctg gaggaaacac tggccgaagt ggaagtcaat ggaactcctt
    10141 accaggtgac gattgacaaa cccctaaaga aaagcgcacc tgtccagttg gtacgggcac
    10201 cacgcgccgc tgcaccttcg gcagcaaaaa cctcgccagt cagcggaaaa gctccctcgg
    10261 caggtggatc ctacactgtc aattccccgc ttcccggagt tatccttgag gtaagtgtaa
    10321 aagaaggcga tactgtcaaa tcaggtcaaa agctgatagt cctggaagcc atgaaaatgg
    10381 aaaacaacat tgaagcagac cgtgatggcg ttgtggcatc tat
//
LOCUS       DAOSOF010000061        10412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6405_length_10412_cov_4.335425, whole genome shotgun sequence.
ACCESSION   DAOSOF010000061 DAOSOF010000000
VERSION     DAOSOF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_10412_cov_4.335425"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLM68_04795"
     CDS             <1..285
                     /locus_tag="PLM68_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT54715.1"
                     /translation="PDRACGPVTVEEYLSRQERYDKQLREEAGIDPSGMTLTEKRNAL
                     RRYREDRYEKLLDAVYKRRGWNNNGVPRVEFLKEIGMDLPELLEVVAPLQ"
     gene            complement(322..2169)
                     /gene="htpG"
                     /locus_tag="PLM68_04800"
     CDS             complement(322..2169)
                     /gene="htpG"
                     /locus_tag="PLM68_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOT54716.1"
                     /translation="MKNKGTIGVTTGDIFPVIKKFLYSDHEIFLRELISNAVDATQKL
                     KALAAKGDFKGEMGDLTIRVSSDKDKSMITVSDRGIGMTEEEVEKYINQIAFSSAGEF
                     LEKYKDQENNIIGHFGLGFYSSFMVAKKVEIVTKSWKEGAQAVRWTCKGTPEYTMEPA
                     DKQDRGTDIILHLYAEYQSYAQESKINELLNKYCSFLPVEIAFGKEKEWKDGKEVVLD
                     KDRIINDVTPLWTKKPVDLKDEDYKAFYEKLYPLAEEPLFWIHLNVDYPFKLTGVLYF
                     PRIKDKLEVTRNKIRLYCNQVFVTDNVESIVPDFLTLLHGVIDSPDIPLNVSRSYLQS
                     DSNVKKISAHITRKVADRLEEIFKNDRPSLEEKWDNLKLFIEYGMLTEEKFYDRALKF
                     ALLKNTDGKYLSFEEYATLIESNQKDKDKKLVYLYATNTEEQFAYIGVAKDKGYDVLL
                     LDSPLCAHFVNLLESKMKDVRFVRIDSDTPEKLIPKEDIAKPDISEDEEKALRELFQE
                     VLPKEATFTVAFENMGSQQLPVVITRGEWMRRYREMSALGGGMNFMGTMPESYNLVVN
                     FEHPLVQRIRETKDRNLVSQICDLALLANNLLKGEALSTFIKRSVDIIQ"
     gene            complement(2237..5527)
                     /locus_tag="PLM68_04805"
     CDS             complement(2237..5527)
                     /locus_tag="PLM68_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HOT54717.1"
                     /translation="MRKRTILTAAIIILFYVGAKAQNHSFPPLLDSTATLKLADSLKT
                     ILSDGVMRISGIRLGSVVLDERHQHITCNFTTGMAEFPIRKENAARVFQTILHFLPPQ
                     YNNYNIRVLTDNKDLEELIPAYYDPERRITDKKQVEKNKKEYQAATEISRKAPAKIQP
                     KKGTPQEIALQYKLEKRRASNNLEKLEAISALKKDTRKHNRHQWPDLGPTPLVYKENR
                     PFEITNGLQNRHIALWHSHGLYYDQKLARWEWQRARLFQTVEDLYTMSYVLPFLTPML
                     ENAGATVLLPRERDTQIHEVIVDNDNPESGYNEQAGTYLWRTDEENGQGFAHKKDVYT
                     YFDNPFRMGTYRRIVTYNPDLNKKVVMQPSSAAWIPNIPETGRYAVYVSYASLPISAR
                     DARYTVHHKGGSEVFRVNQQMGGGTWVYLGTFAFDKGRNLGGRVTLSNLSGEVNAIIT
                     ADAVKFGGGTGNIGRFIQDTAILASYGNIEIPPQVSNYPRFTEGARYWLQWAGFADSV
                     YTYYEGEHDYIDDYSSRGRWVNALAGGSEKHPDNPGLKIPIDLSLAFHTDAGVTLNDS
                     IIGTLAIYTRDSDGTELLPTGQSRLTSRDYTDIVQTQIVNDIRALWNPDWTRRGIWNR
                     SYAESRMAQVPAMLLELLSHQNLADMRYGLDPMFRFTVSRAIYKGMLRYLSSTLGFEY
                     VVQPLPVQSMAVDFSESGQALLTWKPTTDPLEPTAEPLGYVVYTRTGDEKESFDQGVF
                     VLENKAEITLREGIHYSFRVTAVNRGGESFPSETVSLYKAPAGEEKGKILIINGFTRV
                     SAPDSFASDDSLFAGFQDYSDHGVPYLADISYTGSQYEFRRKNRWADDDAPGFGASHA
                     DWETKVIPGNTFDYPIIHGKSFARAGYSYVSSSLRAFTEDYPAYDATDFFALDLILGK
                     QKQVHRGGTDNGTHAFKAFPQALQRKLTEYAQRGGNMIITGAYVARELWDETGKDTLS
                     RRFAMETLKFRFRSGFGSKTGEIMVAPSPITAFYRGTTLGNTTYSFRTGLNPYIYPVE
                     SPDALEPADEGAFTIFRYRDNRLSAGVAYRGDYRIVTLGFPLETLETEEQQERLVKEC
                     MDFFNTDK"
     gene            5571..6710
                     /locus_tag="PLM68_04810"
     CDS             5571..6710
                     /locus_tag="PLM68_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HOT54718.1"
                     /translation="MKLRIPLLVLALSLVLLILFRLFPSFMDTIYSSFLYVVIAQGVS
                     FITALIPFSLAEILIYLLFLIVVFFLVRGILLPAFKPFAEVKVIWKKNLHDAAYLALW
                     GTSFFLLGSGLHYHRLPLEDHLGYPQQQVHPSDLVNLAEALAGEVNRAASFTRRNAEG
                     DMIPEHTFDRYKKDIALAYDSLAFTSGLRVGGWYPATKTVLASRGMSYAYVSGFFFPW
                     TLEANVNKDVPVFSLPAVMTHEQAHARGFMRENEANFLTYLIARHTLSGDLKYSCLLH
                     SLGYVLNAVYSTSPEEYFALLSGLSPRVLYDLKKKHAYWEQFSSSFGEISQKVNDAYL
                     KANAQAGGVATYGKVVDLMVADFKINKEQVQQNPERSVYIGEGTH"
     gene            6720..7250
                     /locus_tag="PLM68_04815"
     CDS             6720..7250
                     /locus_tag="PLM68_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT54719.1"
                     /translation="MNQTALYREYHKPIYHTCLRILRSPMDAEECMQDAFVKLFTGGK
                     MRFINNRACYSWLRKVAVRGAIDRLRSLDFQFSRDAEPIDSTLLKGTASDQEANLFRL
                     QEEEHDKETLVRIIREQMDALPSGYRTILSLYLFEGYDFEEIAEITGLQPVSVRSQYS
                     RGRIKLQSAINKVWTN"
     gene            7238..7741
                     /locus_tag="PLM68_04820"
     CDS             7238..7741
                     /locus_tag="PLM68_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54720.1"
                     /translation="MDKLKSFIQDNKTLFETESLSQEHEKRFMERLAKSQSIKKFRTY
                     KYLIYISSAALLIVVLAIGLRFLKEDPLAVFPSDPCRDGSHICYYNQIRKLSSRIEYD
                     SRNLPEYKRQEIIMNIQSLVPSSAHEFSDMLPADISDKEADRLNIEYYKQLYEGMKEI
                     ASLTNQT"
     gene            7747..8568
                     /locus_tag="PLM68_04825"
     CDS             7747..8568
                     /locus_tag="PLM68_04825"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOT54721.1"
                     /translation="MKRIALITCALVSCTFLFGQNTVKKDYRLESFNSIDASFIYEIE
                     VFKGRDHTVKLEVPEELVDHIQVYVSKNTLYLGVTKEWWRTAKHVLGDRQKIKAAVIM
                     NDLEGFQLSNSASLVTKEGFSPANFMAKLTGASKAEISIITHAANLDISGASELEISG
                     LATKTKAYVSGASIMRYNQETDQIELKASGASTVVMNGSSTSASLTVSGASHLKALDF
                     ETKETDIKCSGASSAKVHVTKEIGVSATGASAVHIKGNPKFTRSVSSGASSINTY"
     gene            8606..9445
                     /locus_tag="PLM68_04830"
     CDS             8606..9445
                     /locus_tag="PLM68_04830"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOT54722.1"
                     /translation="MKKIVTIIIISLFSTMTWAQQGSLEKRNFQVGAFSGIEVSGVVK
                     VNVAKSPVHSLSVETYDDVFQYLDIEVRDGNLHIGVVNGGIPKQVQRKYKGLDIECEV
                     TLPELRSIEMSGVTKMYVRDPFATGVMKIELSGVTYAELKQITCDNLDIEISGVSEIQ
                     LHGITDQLDLEISGASKGYFGLEGKTITFAEIEISGSGNTRMQGKAIRSSIDISGTGA
                     FEGKEFEVEVMKAVLSGVAKAQVKVLGSLEPKISGAAKLHYNKNVSLRNVNTSGAAQM
                     TSY"
     gene            9456..>10412
                     /locus_tag="PLM68_04835"
     CDS             9456..>10412
                     /locus_tag="PLM68_04835"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT54723.1"
                     /translation="MTAKEIHEKILVLDSHCDTPIMLRRHGLDIGYRSETGHVDLVRM
                     KEGSVDASFFAIYVSNKWDPFECTFKALEALASVYDMLERHPDKVALATDARTARELK
                     KQGRIAIFLGMENAGPIHKDLSLLRLFHRMGVRYITLTHAGNNEVCDSCATKEKHWNG
                     VSPFGRELIAEMNRLGILVDVAHISDDAFYDVLKYSTRPVVSTHSCCRALCDVPRNLT
                     DQMIKDLAAQGGVVQINFNPPFLDKAFSLAVAPLLDAYEEAEEAYKDNPSDNEKRMQE
                     AKKALFALPRPSYKRVVDHIDHVVSLVGPDHVGIGSDFDGIEV"
BASE COUNT         2669 a         2412 c         2500 g         2831 t
ORIGIN      
        1 cctgacaggg cctgtggacc tgtgaccgtg gaagaatacc tgtcaagaca ggaaagatac
       61 gacaagcagc ttagggaaga agctggcatt gatccctccg gtatgacgtt gacagaaaaa
      121 cgcaatgcat tgcgccgcta cagggaagac cgttacgaga aactcctgga tgctgtgtac
      181 aagcgccgtg gctggaataa caacggggta cccagggtgg aatttctgaa agagattgga
      241 atggacctgc ctgaattact ggaagtagtt gccccgcttc agtagtaaag gttcattagt
      301 tgtaaaaagg tgttgacacc atcattggat gatgtcaaca cttcttttta taaacgtgct
      361 gagggcttct ccctttagca ggttattggc cagcagggcc aggtcgcaga tctggcttac
      421 caggttcctg tcttttgttt ctcgtatcct ttgcaccagg ggatgctcaa aattcaccac
      481 caggttataa gattcgggca tggttcccat gaagttcatg cctccgccca atgccgacat
      541 ttcccggtaa cgtctcatcc attctcccct ggtaatgacc acaggcagct gttgtgagcc
      601 catgttctca aatgccactg taaaagtggc ttctttgggc agtacttcct ggaaaagttc
      661 acgcagggct ttttcttcgt cttcgctgat atccggtttg gcaatatctt cctttggaat
      721 cagtttttca ggagtatccg agtcaatacg tacaaagcgg acgtctttca tttttgattc
      781 cagcaggttc acaaagtggg cgcaaagagg tgaatccagc agcaacacat cgtatccctt
      841 gtctttggcc accccaatgt aggcaaactg ctcttccgtg tttgtagcgt acaggtatac
      901 aagctttttg tctttgtctt tttggttgga ttcgataagg gttgcgtatt cctcgaaact
      961 aaggtatttc ccatcggtgt ttttaagcag ggcaaactta agggcccggt cgtagaattt
     1021 ttcttccgta agcattccat attcaatgaa gagcttaagg ttgtcccatt tttcctccag
     1081 agagggcctg tcgtttttaa agatctcttc aagccggtct gcgaccttgc gtgtgatatg
     1141 ggcagagatt ttcttcacgt tggaatcgct ctgcaggtaa ctcctggaga cattgagcgg
     1201 gatatccggt gaatcaatca caccgtgcag aagggtaaga aaatcaggta caatgctttc
     1261 cacgttatct gtgacgaaaa cctggttgca ataaagccgg atcttattgc gtgtaacttc
     1321 cagcttgtct tttatcctgg gaaaatacag caccccggtc agtttgaatg gataatccac
     1381 attcaggtgt atccagaaca ggggctcttc cgccaggggg tatagttttt cgtaaaaagc
     1441 cttatagtcc tcatctttca ggtctaccgg cttcttggtc cagagggggg tcacgtcatt
     1501 gatgatccgg tctttgtcca ggactacctc tttgccgtct ttccactcct tttctttccc
     1561 gaaggcaatc tctaccggaa ggaaactgca gtatttgttg agtaattcat tgattttact
     1621 ttcctgagca tacgactgat actccgcgta gaggtgcagg atgatgtccg ttcccctgtc
     1681 ttgcttatcg gccggttcca tggtgtattc gggagttccc ttacaggtcc agcggactgc
     1741 ctgtgctccc tctttccagc tctttgtgac aatttcgact ttttttgcca ccatgaaact
     1801 ggaataaaaa cccaatccaa aatgtcctat aatgttgttt tcctgatctt tgtatttttc
     1861 caaaaattca cctgcactag aaaaagcgat ctggttgata tacttttcca cttcttcttc
     1921 cgtcatacct ataccacggt cagacacggt aatcatagac ttgtccttgt ctgagctcac
     1981 cctgatggta aggtcaccca tttcgccttt gaaatcacct tttgctgcca gtgccttaag
     2041 tttttgagtg gcatctactg cgttggaaat caactctctg agaaaaattt cgtggtctga
     2101 atagagaaat tttttgataa ccggaaaaat atccccggtt gtaacaccaa tggttccttt
     2161 atttttcata attgtcctgt tgttgaagat tatgacccca ggaagtcaaa aatcgttcca
     2221 atgacagaaa tgatgactat ttgtcagtat tgaagaaatc catgcattct tttaccaggc
     2281 gttcctgctg ctcttctgtt tccagggttt ccagcgggaa ccccagggta acaatacggt
     2341 aatcaccacg gtaggcaaca cctgcgctca gccggttgtc cctgtagcga aatatcgtaa
     2401 acgcaccttc atctgccggc tcaagggcat ctggagattc caccggatat atgtagggat
     2461 tcaggcctgt acggaaagag taagtcgtgt ttcccaaagt agttcccctg taaaaggctg
     2521 tgatcgggga gggggcgacc atgatctctc cggtttttga cccgaagccg gagcgaaaac
     2581 ggaatttcag ggtctccatg gcaaaacggc gtgacagggt gtcttttccg gtttcatccc
     2641 aaagttctct tgccacgtaa gccccggtaa tgatcatgtt gcctcctcgt tgggcatact
     2701 cagtgagttt acgctgcaag gcctgtggaa atgccttgaa agcgtgcgtt ccgttatctg
     2761 tccctccgcg atgcacctgc ttttgttttc ccaagatcag gtccagtgcg aaaaaatccg
     2821 tggcatcata tgcagggtaa tcctcagtaa atgcccggag gcttgaagaa acgtaactgt
     2881 atcccgcacg ggcaaaggac ttgccgtgta tgatggggta gtcaaaagta ttacccggaa
     2941 tcactttggt ttcccaatcg gcatgacttg cgccgaatcc gggggcatcg tcgtccgccc
     3001 atctgttttt gcggcggaac tcatattggg aacccgtgta ggaaatgtcc gccaggtatg
     3061 gaacaccatg atccgaatag tcctggaaac cggcaaatag tgaatcgtcc gaagcaaaac
     3121 tatctggagc cgatacgcgg gtgaatccat tgattatcaa tattttaccc ttttcctccc
     3181 ctgcaggggc cttgtacagg ctcaccgttt cagaagggaa gctttctccg ccccggttta
     3241 cggccgtgac acggaaactg taatgtatcc cttcgcgtaa cgtgatttcg gccttattct
     3301 ccaaaacgaa aactccctga tcaaaggact ctttttcatc tcctgtccgg gtatagacca
     3361 cataccccag gggttcggcg gtaggttcca gcggatctgt agtgggtttc catgtcagca
     3421 atgcctgccc gctttcagaa aaatccactg ccatgctttg gacaggaagg ggttggacca
     3481 cgtattcaaa tcccagggtg ctgctcagat aacgcagcat tcccttgtaa atggcacggc
     3541 ttacggtaaa gcggaacata gggtccagcc cgtaacgcat atcggccagg ttctggtggg
     3601 aaagcagttc caggagcatg gcagggacct gtgccatacg gctttcagca taggaacggt
     3661 tccagatgcc ccttcttgtc cagtcgggat tccacagggc acggatgtca ttgactatct
     3721 gggtttgcac tatgtctgta taatccctgg aagtgagacg gctttgaccc gttggcaaca
     3781 attctgttcc atcactatcg cgggtatata tggccaaagt cccaattatg ctgtcgttca
     3841 aggtgacacc ggcatcggtg tgaaaagcaa gggaaaggtc aataggtatc ttcaagcctg
     3901 gattgtcagg gtgcttttcc gatccgccgg ccagggcatt cacccagcgc cccctggaag
     3961 aatagtcatc tatgtaatca tgttcacctt cataataggt ataaaccgag tcggcaaatc
     4021 ctgcccactg cagccagtaa cgagcacctt cggtaaacct ggggtaattg ctcacctggg
     4081 gagggatctc aatattgccg tatgaagcaa ggatggcagt gtcctggata aaccgtccga
     4141 tatttcctgt tcctccgcca aattttacag catcggcagt gatgatggca ttgacttcac
     4201 cgctaagatt ggatagtgta acccggcccc cgaggttcct gcctttgtca aaagcaaaag
     4261 tccccaggta gacccaggtc ccgccaccca tctgctggtt aacacggaac acttcgctgc
     4321 ctcccttatg gtgaactgta taacgcgcat cgcgggcgct gattggaagg ctggcataag
     4381 acacataaac ggcgtacctt cctgtttcag gaatattcgg tatccatgca gcagaagaag
     4441 gctgcatgac aaccttttta ttcaaatcgg gattgtacgt gacgatcctg cgataggtac
     4501 ccatacggaa aggattgtca aaataggtgt atacatcctt tttatgggca aaaccctggc
     4561 cgttctcctc atcggtacgc cacaggtatg ttccggcctg ttcgttgtag ccgctttccg
     4621 ggttatcgtt gtccacaatg acctcatgga tctgggtgtc cctttcacgt ggtaacaata
     4681 ctgttgctcc ggcgttttcc agcatgggag taaggaaagg aagcacataa gacatggtgt
     4741 acaaatcttc aacagtctgg aacagcctgg ctcgctgcca ttcccaacgg gccagtttct
     4801 ggtcgtagta caatccatga ctgtgccata gtgcgatatg cctgttttgc aatccattgg
     4861 tgatttcaaa cggacggttc tccttgtaaa caaggggtgt cgggcccaga tccggccact
     4921 ggtgcctgtt gtgtttcctg gtgtcttttt tcaatgcaga gatggcttcc agcttttcca
     4981 gattattgga ggcccgccgt ttttccagtt tgtactgcaa ggcgatttcc tggggtgtcc
     5041 ctttcttcgg ctggatcttt gccggtgctt ttctgcttat ttcggtggca gcctggtact
     5101 cttttttgtt tttttctacc tgttttttgt cagtgattct acgttcggga tcataatagg
     5161 cagggatgag ttcttccagg tccttgttgt ctgtcaggac cctgatgttg taattgttat
     5221 attgcggagg caggaaatgg agtatggtct gaaaaaccct tgctgcattc tccttcctta
     5281 ttggaaattc ggccatacct gttgtaaagt tgcatgtgat gtgctggtgc ctctcatcca
     5341 ggacgacact tcccaaacgt ataccggata tgcgcatcac tccgtcagag aggatggttt
     5401 tgagcgaatc ggcaagtttt aaggtagctg tgctgtcaag gagcggagga aaggaatgat
     5461 tttgtgcttt ggccccgaca tagaaaagaa ttatgatggc agccgtcaga atggtccttt
     5521 ttctcatggt cttttaataa tgaaaggaca aatataataa ctttgtctat atgaaacttc
     5581 ggataccgtt acttgttctg gccctatccc tggtgcttct gatcttgttc aggctgtttc
     5641 cttcctttat ggacacgata tattccagtt ttttgtatgt ggttatagca cagggagttt
     5701 cattcattac tgcgttgatc cccttttcac ttgccgagat tctcatatac cttcttttcc
     5761 tcatcgtcgt ctttttcctg gtccggggga tcctgcttcc ggctttcaaa ccatttgccg
     5821 aggtgaaggt tatctggaaa aaaaacctcc atgatgcagc gtacctggca ctgtggggaa
     5881 cctccttttt tcttcttggc agcgggcttc attaccaccg gcttcccctg gaagaccacc
     5941 ttggttatcc gcaacaacag gtacacccgt cagatctggt caatctggct gaagcgcttg
     6001 ccggtgaagt caaccgggca gcttcgttta caaggcgtaa cgcggaagga gacatgatcc
     6061 ctgaacacac ctttgaccgg tataagaaag atatagccct tgcctatgac tctcttgcct
     6121 tcacttcggg actcagggtg ggaggttggt atcctgcaac caagaccgtt ctcgcttcca
     6181 ggggcatgtc ctatgcctac gtgagtggat tcttcttccc ctggacgctc gaagccaatg
     6241 tgaacaaaga tgtgcctgta ttctctttac cggccgtcat gactcacgag caagctcatg
     6301 cacgcggttt catgcgcgag aatgaggcta atttcctaac ctatcttatt gcacgccaca
     6361 ctcttagtgg cgatttaaag tattcctgcc tgctgcattc cctgggttat gtattgaatg
     6421 ccgtatacag cacctcccct gaagaatatt tcgcgttgct ttcaggcctg tctccccggg
     6481 ttctgtacga cctgaaaaag aaacatgctt actgggagca attcagttct tcgtttggag
     6541 aaatatccca aaaagtcaac gatgcttacc tcaaggcaaa tgcgcaagcc ggaggagttg
     6601 caacctacgg gaaagtggtg gaccttatgg ttgcagattt taaaattaat aaagaacagg
     6661 tgcaacaaaa tcctgaaaga tccgtttata ttggtgaagg cacccattag cggatgaata
     6721 tgaaccagac agccctatac agggaatatc acaagccgat ttaccacaca tgtttgcgta
     6781 ttttacgctc tcccatggat gccgaggaat gcatgcagga tgcctttgtg aaattgttca
     6841 ccggtgggaa aatgcgtttt atcaacaaca gggcctgcta ttcctggtta cgcaaggtgg
     6901 ctgtcagggg agcgattgac cgccttcgtt ctttggattt tcaattttcc agggatgcag
     6961 aaccaattga cagcaccctg ttgaaaggta cggcctctga tcaggaagcg aaccttttcc
     7021 ggctacagga agaagaacat gacaaggaaa cccttgttcg gatcataagg gaacaaatgg
     7081 atgcccttcc atcgggttac cgcaccattc tgagcttata tctttttgaa ggatatgatt
     7141 ttgaagaaat tgcagaaata acaggactgc agcctgtttc agtaagaagc caatacagcc
     7201 gtggcaggat aaaactacag tcagcaataa acaaggtatg gacaaattaa agtctttcat
     7261 tcaggataac aagacacttt tcgaaacgga aagtctttcg caggaacatg aaaaaaggtt
     7321 catggaacgc ctggcaaaaa gccaaagcat taaaaagttc cgaacttata agtatttaat
     7381 ttacatatct tctgcagcat tacttatagt agtgcttgca ataggattac gcttcctgaa
     7441 agaggatccg ctggctgttt ttccttccga cccgtgcaga gacggatccc acatctgtta
     7501 ttacaaccag atccggaagc tttccagtcg gattgagtac gactccagga acctgcctga
     7561 atataaacgg caggagataa tcatgaatat acaatccctg gtgccatcat ctgcccatga
     7621 attttcggat atgcttccag cagacatatc tgataaagag gcagaccgct tgaacataga
     7681 gtattataaa cagttatatg aaggaatgaa agagattgcc tcattgacaa atcaaacgta
     7741 aaaaacatga aaagaattgc attaatcaca tgtgcactgg tatcgtgcac tttccttttt
     7801 ggacaaaaca ctgttaaaaa agattaccgg cttgaaagtt ttaattccat tgacgcttct
     7861 ttcatttatg agattgaagt tttcaagggc agagaccaca cggtgaaact ggaagtaccc
     7921 gaagaactgg tagatcacat ccaggtatat gtttcaaaga acacacttta cctgggagtg
     7981 acgaaagagt ggtggcgtac cgcaaaacac gtcctgggcg acaggcagaa aatcaaagcc
     8041 gctgtcataa tgaacgatct ggaaggattc cagttgtcaa attcagcaag tcttgttacg
     8101 aaggagggat tttcgcctgc caatttcatg gcaaaattga caggtgcatc aaaagctgaa
     8161 atatctatca taacacatgc tgccaacctt gatataagcg gggcatctga actagagatc
     8221 agtggtttgg caacaaaaac aaaagcctat gtcagcggtg catccatcat gaggtacaac
     8281 caggagactg atcagatcga attgaaggcg tcaggcgcct ctacggttgt gatgaatgga
     8341 agttcaacaa gcgccagtct gacggtttcg ggcgcaagcc atctgaaagc ccttgacttt
     8401 gaaacgaaag agacggatat taaatgttcc ggagcttctt ccgcaaaagt ccatgtgact
     8461 aaagagatcg gtgtatctgc taccggagca tctgctgtac acataaaagg caatccgaaa
     8521 tttacccggt cggtatccag cggagcttct tctatcaata cttattgata tatagtatta
     8581 atttagataa ataaaataaa atgctatgaa aaaaatagtt acaattatta taatatcgct
     8641 tttttccacg atgacatggg cccagcaggg gtccctggaa aaaaggaatt tccaggtagg
     8701 agctttttcg gggatagaag tttccggtgt agtaaaggtt aacgtggcta aaagtcctgt
     8761 tcactccctg tcagtggaaa catatgatga tgtttttcag tacctggaca tagaagtgcg
     8821 tgacgggaac ttgcatatag gggttgtcaa cggaggtatt cccaaacagg ttcagcgtaa
     8881 atacaagggc ctggatattg aatgcgaagt gacactccct gaactcagga gcatagaaat
     8941 gagcggcgtg acgaagatgt atgttaggga tccttttgca accggcgtaa tgaagataga
     9001 gctgtccgga gtcacctatg cagaacttaa gcagataaca tgcgacaacc tggatatcga
     9061 gatctctggt gtatctgaga ttcagcttca tggcatcaca gaccagctgg accttgaaat
     9121 aagcggggct tccaagggtt atttcggttt ggagggaaaa accattacct ttgccgagat
     9181 tgaaataagt ggaagcggaa ataccaggat gcagggtaaa gccatacgtt cttcgattga
     9241 tataagcggg accggggctt ttgagggaaa ggaattcgaa gttgaagtta tgaaagccgt
     9301 tttaagcggc gtggcaaaag cccaggtaaa ggttttgggc tcgcttgagc cgaagatatc
     9361 aggagctgcc aaactgcatt acaacaagaa tgtatccctg cggaacgtta acaccagcgg
     9421 cgctgcccaa atgacatctt attaaatcct tatatatgac agccaaagag atccatgaaa
     9481 aaatactggt actggattca cattgcgata cacccataat gctcaggagg catggcctgg
     9541 atataggtta cagatccgaa acaggccacg tggatcttgt aaggatgaaa gaaggaagcg
     9601 ttgacgcgtc cttctttgct atttatgtat ccaataaatg ggatccgttc gaatgtacct
     9661 tcaaggcact tgaagcactt gcctctgttt acgacatgct ggaacgccat cccgacaaag
     9721 tggccctggc aacagacgcc cggacggcca gggaattgaa aaagcagggc aggatagcca
     9781 ttttcctggg tatggaaaac gccggtccca ttcacaagga tctttcctta ctgcgcctgt
     9841 tccacaggat gggtgtgcgc tacataaccc tgacacatgc gggtaacaac gaagtatgtg
     9901 acagttgtgc caccaaagaa aagcactgga acggggtaag tccttttggc agggaattga
     9961 ttgccgagat gaaccgtctg ggaatcctgg tggatgtcgc gcatatttct gatgatgctt
    10021 tttatgatgt tctcaagtac tccacccgtc cggttgtatc cacccattct tgttgcagag
    10081 ctttatgtga tgtgcccaga aaccttacag atcagatgat taaagatctt gcagcacagg
    10141 gtggggtggt ccagatcaac ttcaaccctc cttttcttga caaggccttt tcgctggctg
    10201 ttgccccatt actggacgcc tatgaagaag ccgaggaagc atacaaggat aatccctccg
    10261 ataacgaaaa acgcatgcag gaggcaaaaa aagccctttt tgcacttccc cgaccatcct
    10321 ataaaagggt ggtcgaccat attgatcatg tcgtctccct ggtaggtccg gatcatgtag
    10381 gcattggttc tgactttgac ggcatagagg tt
//
LOCUS       DAOSOF010000062        10306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6485_length_10306_cov_4.616233, whole genome shotgun sequence.
ACCESSION   DAOSOF010000062 DAOSOF010000000
VERSION     DAOSOF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6485_length_10306_cov_4.616233"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLM68_04840"
     CDS             complement(<1..901)
                     /locus_tag="PLM68_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54724.1"
                     /translation="MKMLRYIWDRKFLIPLILAVICLTLSFFELTSHNRRMMRLVNVE
                     KILHKKQRKIEAIALHELEQEKMGVLSPKDVARDMVVYKYYKDSLISWVNTFPVINDS
                     YSPGTMFPNLSFFSRNTIIHQPLSEVDGREQYLNLGSAWYVVRAYSREDVTIITGILI
                     RTDFPFSNSFVESKVNKALRLPVHCSIVPLNLDEGFIVFGKDNGVLFTILSDISMQTF
                     SDSVILRWIAITLVLLSMLMFLLRNRQVISAIGVIAALCLLRFLTYIYSSSLQLDTLF
                     FSPLLFAGGPMENSLAAMIINTLC"
     gene            complement(898..1371)
                     /locus_tag="PLM68_04845"
     CDS             complement(898..1371)
                     /locus_tag="PLM68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT54725.1"
                     /translation="MNILLLCMGKTQEPYLREGILDYEKRLIRYVRFKRLELPDVKST
                     SSLSFNQIKEKEGNLLLKNVKVSDHLILFDEKGCAYSSKQMSYQIEQHLQRGTRDLVF
                     AIGGSFGFSPDVINRANEMWSLSKLTFPHQLVRLLCVEQLYRCFTIIKGEAYHHE"
     gene            1422..1832
                     /locus_tag="PLM68_04850"
     CDS             1422..1832
                     /locus_tag="PLM68_04850"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOT54726.1"
                     /translation="MIRKTILPLMLACFSAFAPNFLAAQDAELTEIFNNIGNDLKSGD
                     AVTFSQWFADDIEIDVMGTAGVCSRSQARQLMKNFYTKYTPKSFSIVHLSGTLPMRYC
                     IGTLLAGGERFRVTLFVKSEREMHKLQQVRIEKD"
     gene            complement(1834..3138)
                     /gene="der"
                     /locus_tag="PLM68_04855"
     CDS             complement(1834..3138)
                     /gene="der"
                     /locus_tag="PLM68_04855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT54727.1"
                     /translation="MGIVAIVGRPNVGKSTLFNRLAGKRQAIVDETAGVTRDRHYAKT
                     DWNGREFSIIDTGGYAHNTDDIYEEQIRRQVLIAIEESDVVVFMVDVQTGITDYDQSI
                     ARILRKSEKPVILTVNKVDHGGRIFDTYQFNALGLGEPISIAAASGSGTGELLDRILQ
                     ELPGNSTVEEEHDIPHIAIVGRPNVGKSSLTNAFLGEERNIVTPVAGTTRDAISARYN
                     KFGYDFMLVDTAGLRKKTKVREDLEFYSAMRAVRAIEFADVCILMLDATSGVESQDMS
                     IFQLIVKNKKGCVVVVNKWDLLEKETNTMKEYTKGILARMAPFSDVPVIFTSVVKKQR
                     IFDVLEAAAKVYENRTRRISTSMLNQVLLPEIEAFPPPSIKGKYIKIKYITQLHSPCP
                     SFAFFCNLPQYVREDYKRFLENKLRAHFDFKGTPVQVFMRKK"
     gene            complement(3319..4155)
                     /gene="nadE"
                     /locus_tag="PLM68_04860"
     CDS             complement(3319..4155)
                     /gene="nadE"
                     /locus_tag="PLM68_04860"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT54728.1"
                     /translation="MKTPEELYLVLTGGIRRFFSDAGKTNAVLGISGGLDSAVVSCLA
                     QDALGKEQVCGLLMPGPFSTVHSLNDAMELCMLNGIPNYIIPIDQIYHKFLRELSPVF
                     EHDTHGVMEENLQARIRGVLLMAYANKKDCLLLNTSNKSELAMGFGTLYGDLAGALMV
                     LADVYKTSVYDLAAFLNRDEERIPAHTISKEPSAELYIDQKDTDSLPEYDVLDPILHA
                     LIEEGKTEEELLDSGTNPELLAGITHKMRLSGFKALQVPPLLAVTDHPLLPPDKSLRY
                     DL"
     gene            complement(4155..5105)
                     /locus_tag="PLM68_04865"
     CDS             complement(4155..5105)
                     /locus_tag="PLM68_04865"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT54729.1"
                     /translation="MDHLHLFKIFSCRGSRYLAERIASHLGLPLGLLSVTTFSDGEFQ
                     PAFDESVRGATIFIIQSTFPPVDNLFELLLTIDAAKRASAYKVIAVMPYFGWARQDRK
                     DRPRVSIGAKMVANLLEAVGCDRVMTMDLHADQIQGFFDFPVDHVYSSSIFVPYIKSL
                     GLENLSVAAPDMGGAKRANAYSRKLECPLIICHKSREKANVVGSMTAIGEVADRNIII
                     LDDMIDTAGTITKAAEMMMSKGARSVRAIATHPVLSGLAYSTIANSSLEEVIVTDTIP
                     LSCNPDVNKDKIKVLSIAREFADIIDKVYNFQSISSKFTL"
     gene            complement(5154..5753)
                     /gene="yihA"
                     /locus_tag="PLM68_04870"
     CDS             complement(5154..5753)
                     /gene="yihA"
                     /locus_tag="PLM68_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT54730.1"
                     /translation="MEIKEASFLSATTNVDEIPIPELEEFAFIGRSNVGKSSLINMLC
                     NRRKLAHTSSTPGKTQCINRFLVDQGWIITDLPGYGFAKVSKTKRAALEAMINGYIRR
                     APGMTLLFVLLDARHPLQQIDLDFLIRLGNWQVPFAIVLTKSDKLSKTALERQSASLR
                     ESVSQYWDPLPPFFVVSSLTGEGRKELLDYIGSIRSKKF"
     gene            5799..6965
                     /gene="xseA"
                     /locus_tag="PLM68_04875"
     CDS             5799..6965
                     /gene="xseA"
                     /locus_tag="PLM68_04875"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT54731.1"
                     /translation="MEKMDLIDLQRILEEAISQAFEEPCWVRAEINQVSVRNGHCHLE
                     LVQKEEVTDKLLAKAQAVIWSSVYSILNPYFRTSTGMDFRSGLQVLLRVEPRMHPLYG
                     LSLVVTDADPSYTIGGLELERRKTMERLEQEGILDMNRQLPFPPVPLRLAVISSEAAA
                     GYQDFRDHIGLSRYGFSITLFPALMQGSDAPSSIISAMDQVIGSGSDFDVLVMIRGGG
                     AATDLHCYDNYDLAANIAQFPIPVITGIGHHKDVHIADRVAHLALKTPTAVADYLDGH
                     LAAEEEGLFRLSHNLERTVQRRIEDEKQSLNYFLRDIGGAVRWLLQQQEHLLELMEQR
                     IKHNNPMNLLNKGYSLTLFRGKAVLDASLLKEGDKVQTILSKGSFGAVVHDIEN"
     gene            6971..7177
                     /gene="xseB"
                     /locus_tag="PLM68_04880"
     CDS             6971..7177
                     /gene="xseB"
                     /locus_tag="PLM68_04880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT54732.1"
                     /translation="MNRKKEITYLQARQELQELVEELEKPDADLGQIAAKVKRALELV
                     TFCRNYLRKVQEDTEAIIETHEKD"
     gene            7164..8339
                     /locus_tag="PLM68_04885"
     CDS             7164..8339
                     /locus_tag="PLM68_04885"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HOT54733.1"
                     /translation="MKKIDKGTLVAFILLFAFTSLQKLEAQGRARIMDTDTVTNSLIR
                     QLREKTSEIFHDPAPPRFVMIDKSERFLFGMGGYAACYTYYDGTGFLSRDMSAVLIPT
                     ESYKLRGGRFNLDASGSRLFFKLIGDTSIGKLEAYIEGGFSGEGYTFSLKHAYVEIRH
                     LLIGHTWSNIMDLRAAPATVDGAGPGGMNGLRQPQFRYGWRWTNGISCFASLEDPEVT
                     MLKALVTGTNDVHIMEQRFPDLIVSLGYEKPSWHLRLAGVLRFLEKQRGYGLAFSGNA
                     SLTRRDEIKFQVTGGAGIGHYIQDLGGMGYDVYIQRDGSYDKFTALGYYAAYEHHWSE
                     KCFSGLVYSQVYVQPFEQAPGFICHHTNYAAVNTFLDIYEHGQLGMEGVYGNRVNLD"
     gene            8393..>10306
                     /locus_tag="PLM68_04890"
     CDS             8393..>10306
                     /locus_tag="PLM68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT54734.1"
                     /translation="MEKSEIWELDAYLKPFKPVLWRRHQMRLNKALEITGAHDCLAHA
                     VNNHLYYGVHRTDRGWVCREWAPNARAMYLTGECNGWGKDPSYAFTPIGSGNWELVLP
                     PEALRHGQLFKWFIEWPGGAGERLPAYARRCVQDPETKIFSAQIWEPAEAYEWKHCNP
                     PRVGHPLVYEAHIGMSAQEPGIADFDWFRTKVLPRVARLGYNTLQLMAIQEHPYYGSF
                     GYQVSNFFAVSSRFGTPEGFKRLIDEAHAKGIAVIMDLVHSHAVKNELEGLSRFDGTY
                     DLYFHAGPRGEHPLWSSRCFDYGKNDVIHFLLSNIKYWMEEFHLDGFRFDGVTSMMYH
                     DHGMGRDFTQYGDYFDGNQDEDAITYLGLANQLVHECNPCAITLAEDVSGMPTLAYPR
                     EMGGIGFDFRLSMGVADHWIKWIKELRDEDWHVGDIFYQLTNKRRDEHTISYAECHDQ
                     ALVGDKTIIFRLIDKDMYTSMSKDIPSLVVDRGIALHKIIRLLSLATAGDGYLNFMGN
                     EFGHPEWIDFPREGNGWSYHYARRQWSLADDKLLRYRDLELFDRDMIRMAKAQNLYDS
                     PPLPVVQDIGSQVLAFERGRLLFVFNLSPGRSYTGYGMEVRPGKYRCILDTDDPSYGG
                     FGRNDRKTLHVTLP"
BASE COUNT         2648 a         2421 c         2617 g         2620 t
ORIGIN      
        1 cacataatgt attgataatc attgcggcca atgaattttc cataggtccg ccggcaaaga
       61 gcaatgggga aaagaacagt gtatccagtt gcagggatga agaatatata tacgtcagaa
      121 aacgcagcag gcacaaggcg gctatcactc ctatggcaga tatgacctgg cggttcctga
      181 gtaagaacat aagcattgat aacaagacca gggtaatggc aatccaccgg agtattaccg
      241 aatcggagaa agtctgcatg gatatgtcgc tcagaatggt gaaaagcaca ccattgtcct
      301 tgccaaagac aataaaacct tcgtccaggt tcagggggac tatggagcag tgaacaggaa
      361 gccggagggc cttgtttacc tttgattcca cgaaactgtt actgaatggg aaatctgtcc
      421 ggataagaat accggtgatg atggtaacat cttcccggct gtaggcccgt accacatacc
      481 aggcgcttcc caggttcagg tattgctccc tgccgtctac ttccgacagc ggctggtgga
      541 tgatggtatt cctggaaaag aaagacaggt tgggaaacat ggtcccggga ctgtaactgt
      601 cgttgatcac aggaaatgta tttacccagg atatcaggct gtctttgtaa tacttataga
      661 caaccatgtc acgggcaaca tcttttggtg aaagaacgcc cattttttcc tgttccagtt
      721 cgtggagcgc tatggcctcg attttccgct gtttcttgtg tagtattttt tccacattga
      781 ccaggcgcat catacgccgg ttatgtgaag tcagctcaaa gaaagaaagc gtcaggcaga
      841 ttaccgccag gataagcggt atcaggaatt tccggtccca tatgtacctg agcattttca
      901 ttcgtggtgg taagcttctc ccttaatgat ggtgaagcag cggtatagtt gttcaacaca
      961 caataaacgt accagttggt gtggaaaagt aagtttggaa agagaccaca tttcattggc
     1021 tctgtttata acatcgggtg aaaaaccaaa ggagccgcca atggcaaaga ccaggtcacg
     1081 tgtccctctt tgtaaatgtt gttcaatttg gtatgacatt tgctttgaac tataagcaca
     1141 acccttctca tcaaacagaa taaggtggtc agatactttc acgtttttta aaagcaaatt
     1201 accttccttt tctttgatct gattgaagga taatgaagaa gtgctcttga catcgggtaa
     1261 ttcaagacgt ttaaagcgga cataccggat cagccgtttt tcatagtcca gaataccttc
     1321 acgcaggtaa ggttcctggg tcttgcccat gcaaagcagc agaatattca ttaaacaaaa
     1381 gtaagaaatt atgtgtaact tgccgtaatt ttgaatacac tatgatcaga aagacgatct
     1441 tacccctgat gcttgcctgt ttttctgcat tcgcccccaa tttccttgcg gcgcaggatg
     1501 ctgagttgac ggaaattttc aataacatag gcaatgatct gaaaagcggc gatgcggtta
     1561 ctttttcaca gtggtttgcc gatgatatag aaatagatgt gatgggaact gccggtgttt
     1621 gcagcaggag ccaggcccga cagctgatga agaatttcta tacgaaatat actcccaaat
     1681 cattctccat agttcatctg agcggcacat tgcccatgcg ctactgtata ggcaccctgc
     1741 tggccggagg cgagcgtttc cgtgtgacat tgtttgtcaa atcagaacgg gaaatgcaca
     1801 aactgcaaca ggtccggata gaaaaggact gatctatttc tttctcatga aaacctgtac
     1861 cggagttccc ttgaaatcaa agtgagcacg taatttattc tccagaaacc gcttgtaatc
     1921 ctctctgacg tattgaggca ggttgcagaa aaatgcaaaa gagggacagg gtgaatgcaa
     1981 ttgtgtgatg tatttgatct tgatgtattt tcccttgatt gaaggtggag ggaatgcctc
     2041 tatttcgggt aacaatacct ggttcagcat ggaagtggat atgcggcgtg tacggttttc
     2101 ataaacttta gcggcagctt ccagcacatc aaagatgcgt tgttttttca ctactgaagt
     2161 gaagatcacg ggaacgtccg agaaaggtgc catcctggcc aggatccctt tggtgtattc
     2221 tttcattgtg ttggtctctt tttcaagcag gtcccacttg ttgaccacca ccacacaacc
     2281 tttcttgttt ttcactatga gctggaagat gctcatgtcc tgggattcca cacctgatgt
     2341 ggcatccagc atcaggatgc atacatctgc aaattcaatg gccctgacag ccctcatggc
     2401 ggaatagaat tccaggtctt ccctgacctt tgtttttttt cgcagcccgg ccgtatcgac
     2461 cagcatgaaa tcgtaaccga atttattgta cctcgcactt atggcatcac gggtcgttcc
     2521 cgcaacaggc gtgacaatat tcctttcttc tcccaggaag gcattggtaa gtgaggattt
     2581 gcccacattg ggcctgccca cgatcgctat gtgcgggata tcatgttctt cttccacagt
     2641 actgttgccg gggagctcct gtagtatcct gtccagcagc tcaccggtcc cgctaccact
     2701 ggctgcggct atggatatgg gttctcccag tcccagggcg ttgaactggt aggtatcaaa
     2761 tatccgtcct ccgtggtcta ccttgttgac tgtcaaaata accggttttt cagattttct
     2821 gagtatcctg gcaatgctct ggtcgtagtc tgtgatccct gtctgcacat ctaccataaa
     2881 aaccacgaca tcggattctt ctatggcgat gagaacctgc cgccttatct gttcttcgta
     2941 tatatcgtct gtgttatggg cgtagccgcc ggtatctatt atggagaatt ccctcccgtt
     3001 ccagtccgtt tttgcgtaat gacgatcacg ggtaacgcct gcggtctcat caactatggc
     3061 ctgccgtttt cctgccagtc tgttgaaaag cgtggatttt ccaacattag gccttcctac
     3121 aattgcaaca attcccattc gttccttttt tcagtagcat gctttcttct tgtttcgggc
     3181 agcgtattcc cctttccctc atcttcctgt tatgaccgac agaatattcg ggaaacctcc
     3241 cgtttttcct ggctattatc gtaatactca tccccaggac accaagtaac aacagtccca
     3301 gcgacaggag aaaaacagtc ataggtcgta acgtaaagac ttgtcagggg gaagtaaggg
     3361 atgatcagtc acggccagca gaggtgggac ctgtagggct ttgaatccgg aaagcctcat
     3421 tttatgggtt atacccgcca gcagttcagg atttgtgccc gagtcgagca gttcttcttc
     3481 tgtttttcct tcttctatga gggcatgcag aatcgggtcc agcacatcat attcgggcag
     3541 tgaatcggtg tctttctgat ctatgtacaa ctctgccgat ggctctttgc ttatggtgtg
     3601 tgccgggatt ctttcctcat ccctgttcag gaaggcagcc aggtcataga cagaggtttt
     3661 atatacatcg gcaagaacca tcagggcccc ggccaggtca ccgtacaggg tgccgaagcc
     3721 catggccagt tcactcttat tgctggtgtt caggagaagg caatcttttt tattggcata
     3781 ggccatcagg agcacccccc tgatacgggc ctgtagattc tcctccatca caccgtgggt
     3841 atcatgttca aagacgggcg agagttcgcg taagaatttg tgatatatct ggtctatagg
     3901 aatgatataa ttggggattc cgttcagcat gcataactcc atggcatcgt tcagggaatg
     3961 gacagtggag aaaggcccgg gcatgagcag accgcatacc tgctccttcc ccagggcatc
     4021 ctgggccagg catgatacaa cggcagagtc cagtcctccg gaaatgccaa gcacagcgtt
     4081 cgttttaccc gcgtcactga aaaatcgccg tataccgccg gtcaatacaa ggtataattc
     4141 ctcgggagtt ttcattacaa ggtgaatttg ctgctgatgg actggaaatt atatactttg
     4201 tcaatgatgt ctgcaaattc acgcgctatt gacaatacct ttatcttgtc cttgttcaca
     4261 tcgggattgc aagacagggg tatggtatcg gtcacgatca cttcctcaag cgatgaattg
     4321 gctatggttg agtaggccag tcccgaaagg accggatgtg ttgcgatggc acgaacactg
     4381 cgtgcccctt tcgacatcat catttcagcc gcttttgtta tggtgccggc tgtatctatc
     4441 atatcgtcca gaatgataat gttcctgtcg gcgacttccc caatggctgt catgctaccc
     4501 accacatttg ccttttcacg cgatttatgg cagatgatca ggggacattc cagctttctt
     4561 gaataggcat tggcccgctt ggcaccgccc atatctggag cggcgacaga gaggttttca
     4621 agtcccagac tcttgatata gggtacaaag atagaactgg aatagacatg gtccacggga
     4681 aaatcaaaaa agccctgtat ctggtcggca tgcaggtcca tggtcataac acggtcacag
     4741 cccacagctt ccagaaggtt ggcaaccatc tttgcgccta tggataccct gggcctgtct
     4801 ttacgatcct gcctggccca tccaaaatag ggcatcacgg ctattacctt gtaagccgag
     4861 gcccttttgg cagcgtctat ggtcagcagt aattcaaata aattatccac cggagggaaa
     4921 gtagactgaa taatgaaaat ggtggctcca cggacacttt catcaaaagc tggctgaaac
     4981 tctccgtcac tgaatgtggt taccgataac aatcccaggg gcagacccag atgagaggct
     5041 atacgttctg caagatatct ggacccgcgg caggagaaaa ttttgaataa atgcagatga
     5101 tccatggtcc ctatggtttg aagatgggac aaagataaga agaaaaaaca caattagaat
     5161 tttttggagc ggatggagcc aatataatcc agaagttctt ttctgccttc ccctgtcagc
     5221 gaggaaacca caaaaaacgg aggtagcggg tcccagtatt gggaaacgga ttccctcaaa
     5281 gaagccgatt gtcgttcaag ggcagtcttt gacagcttgt ccgatttggt gagcacgatg
     5341 gcaaaaggga cctgccagtt gccaagccgg atcagaaaat caaggtcaat ttgctggagc
     5401 ggatgccttg catcaagcag tacaaacaac aaggtcatcc ccggggcccg gcggatatac
     5461 ccgttgatca ttgcctccag ggcagccctc tttgttttgg agactttcgc aaatccatag
     5521 ccgggaaggt ctgtaatgat ccagccctga tccacaagaa acctgtttat gcactgtgtc
     5581 ttgccagggg tagaggatgt gtgggccagt ttgcgcctgt tacacaacat gtttataagg
     5641 gatgattttc ccacgttaga ccttccgata aaagcgaatt cttccagttc cgggataggg
     5701 atttcatcta catttgtcgt ggcagaaagg aaagaggctt ctttgatttc catggggcga
     5761 aggtaaggtt ttttactaat ttaatgtaaa tttgtgcaat ggaaaaaatg gatctcattg
     5821 acctgcaaag aatactggaa gaagctatca gccaggcctt tgaagaacca tgttgggtcc
     5881 gggcagaaat caaccaggtg tccgtgcgga atggccactg ccatttggag ctggttcaga
     5941 aagaagaagt cactgacaaa ttgctggcaa aagcccaggc ggtgatctgg tccagcgttt
     6001 attccatcct caatccctat ttcagaacaa gcaccggcat ggatttccgt tccggactgc
     6061 aagtgctttt gcgcgtcgaa cccagaatgc atcctttgta tgggctgagc ctggttgtta
     6121 ccgatgctga tccttcctac accattgggg gtcttgaact ggaacgcagg aagactatgg
     6181 aacgccttga gcaggaaggc atcctggata tgaacaggca attgccattc ccgcccgtac
     6241 ctctccgtct ggcggtgatt tcttcagaag cggctgcagg ataccaggat ttccgtgacc
     6301 atatcgggtt atcccgctat ggattcagta taacgctttt tcccgccctc atgcagggaa
     6361 gcgatgctcc ctcaagcatc atcagtgcca tggaccaggt gatcgggtcc ggaagtgatt
     6421 ttgatgtact tgtgatgatc cggggcggag gggccgcaac agacttgcac tgttacgaca
     6481 actatgacct ggctgcaaac atagcccagt tccccatccc tgtcatcaca gggatcgggc
     6541 accacaagga tgtccatatt gcggacaggg ttgcccacct tgcccttaaa actcctacgg
     6601 cagtggctga ttacctggac ggacacctgg ccgcagagga agaaggcctg ttcaggcttt
     6661 cgcataacct ggaacggaca gtgcaaagaa ggattgaaga tgaaaaacaa agcttgaatt
     6721 actttctccg ggatattgga ggagccgtca gatggctcct gcaacaacag gaacatttgc
     6781 tggaattgat ggaacagcgg ataaagcata ataatccaat gaacctgctg aacaaggggt
     6841 actcccttac cctgttccgg ggtaaggcgg tactggatgc ctctctgctc aaagaaggcg
     6901 acaaggtaca gaccatactt tcaaaaggaa gctttggcgc tgtggtacat gacatagaaa
     6961 actgaatgtg atgaacagga aaaaagaaat aacctacctg caggctcgtc aggaactcca
     7021 ggaattggta gaagaactgg agaaacccga tgcagacctg ggacagatag ctgccaaagt
     7081 gaaaagggcg ctggaactgg tcactttttg caggaattac ctgcgcaagg tccaggaaga
     7141 cacggaagca ataatcgaaa cacatgaaaa agattgacaa aggaacactt gtcgctttta
     7201 tccttctatt tgccttcact tccctgcaaa agcttgaggc acagggaagg gcaaggatca
     7261 tggataccga tactgtcacc aattcgctga taaggcagct aagggaaaaa acctccgaga
     7321 tctttcatga tcccgctccc ccgcgttttg taatgataga caagagtgaa agatttcttt
     7381 tcgggatggg tgggtatgcc gcctgttaca cgtattacga cgggacaggt tttttatcca
     7441 gggacatgtc tgcagttctg atacctaccg agtcttacaa gttgcgcggc ggacggttta
     7501 accttgatgc aagcggttcc aggttgtttt tcaaactcat aggcgacacg tccataggaa
     7561 agctggaagc ttatattgaa ggaggttttt ccggagaggg gtatactttt tcactcaagc
     7621 acgcctatgt tgaaatcaga caccttctca taggacacac ctggagcaat atcatggacc
     7681 taagggctgc cccggccact gtagacggtg cgggaccagg cggaatgaac ggattgcgcc
     7741 agcctcagtt ccgttacgga tggagatgga cgaacgggat cagttgtttt gcctcactgg
     7801 aagaccctga agtcaccatg ctcaaggccc tggtcacggg aacgaacgat gtgcatatca
     7861 tggaacaacg tttcccggac ctgattgttt cgctgggata tgaaaaaccg tcctggcacc
     7921 tcaggttggc aggcgtgctg cgtttcctgg aaaagcagcg tggttacgga ctggcctttt
     7981 cgggaaatgc aagcctgaca cggcgcgacg agatcaagtt ccaggtaaca ggaggggctg
     8041 gcatcggaca ttacatacag gacctgggag gaatgggcta cgatgtgtat attcaaagag
     8101 atggcagcta cgacaaattc actgcactgg gttattatgc tgcctatgaa caccactgga
     8161 gtgaaaaatg cttctccgga cttgtgtatt cgcaggtata cgtgcaacct tttgaacagg
     8221 ctccgggatt tatttgccac catacgaatt acgcagcagt caacacattc ctggatatct
     8281 acgaacacgg tcagcttgga atggaaggcg tgtacggcaa cagggttaac cttgactgaa
     8341 gaaaaggcta ttcctggcgt ttgaattgta ttgtgcgtta taatttctga atatggaaaa
     8401 atcggaaata tgggagctgg acgcttacct gaaacctttt aagccggtat tgtggagacg
     8461 tcaccagatg cgtctgaaca aagccctgga aatcacagga gcgcatgatt gtctggcaca
     8521 tgctgttaac aaccacctgt actacggcgt tcacaggact gaccggggtt gggtatgtcg
     8581 cgaatgggca cccaatgccc gggccatgta tttgacaggg gagtgcaacg gctggggaaa
     8641 agatccctct tatgccttca cacccatagg ttcaggcaat tgggagttgg tgctgccacc
     8701 ggaagcgctg cgccacggtc aactcttcaa gtggttcata gagtggccgg gaggtgcagg
     8761 agagcgtctc cctgcctacg caaggcgctg tgtccaggat ccggaaacca agatattcag
     8821 tgcccagata tgggaacctg cggaagccta tgaatggaaa cactgcaatc caccccgcgt
     8881 gggacatccc ctggtctatg aagcgcacat aggcatgagt gcacaggaac cgggaattgc
     8941 agatttcgat tggttccgca ccaaagtcct tccaagggtc gcgcgcctgg gatacaacac
     9001 cctccagctg atggccatcc aggaacatcc ctattacggt tcatttggct accaggtatc
     9061 caatttcttt gccgtcagct cccgcttcgg cacaccggaa ggcttcaaga ggctaataga
     9121 tgaagcccat gcaaagggta tcgccgtgat catggacctg gttcattcgc acgccgtaaa
     9181 gaatgagctg gaaggtttga gccggtttga cggtacctat gacctttatt ttcatgcagg
     9241 accaagaggg gagcatccct tgtggtcttc ccgttgtttc gattacggga aaaatgatgt
     9301 gatccatttt ctcctctcaa acataaaata ctggatggaa gaattccacc tggacggatt
     9361 ccgctttgac ggggtcactt ccatgatgta tcacgatcat ggcatggggc gtgatttcac
     9421 ccagtacggc gactattttg acgggaacca ggatgaagat gccatcacct acctgggtct
     9481 ggccaaccag ctggtccacg aatgcaatcc ctgcgccatc accctggctg aagatgttag
     9541 cggcatgcca acacttgcct atcctaggga aatgggagga ataggctttg atttccgcct
     9601 gagcatggga gttgctgatc attggatcaa atggatcaag gagctgcggg acgaggattg
     9661 gcatgtgggg gatattttct atcagcttac caacaagcgc agggacgagc acaccatatc
     9721 ctatgccgag tgtcacgatc aggcgctggt tggtgataaa acaatcatat tcaggctcat
     9781 agacaaggat atgtatactt ccatgagcaa agacatcccg tcgcttgtcg ttgacagggg
     9841 aattgccctg cacaagatca tacgtttgct ctcccttgcc acagccgggg acggatacct
     9901 gaatttcatg ggaaatgaat tcggtcatcc ggaatggata gactttccca gggaaggaaa
     9961 cggatggagt taccattatg cgaggagaca gtggtcattg gccgatgata agctgctgcg
    10021 ctaccgggat ctggagcttt ttgacaggga catgatacgg atggccaagg cacagaattt
    10081 gtatgatagc cctccgcttc ccgtagtgca ggatataggg tcacaggtgc tggcgtttga
    10141 acggggaagg ttgttgtttg tcttcaacct gagtccgggc aggtcctata ccggttacgg
    10201 tatggaagtc agaccgggaa aataccgttg catattggat acagatgatc cttcttatgg
    10261 aggattcggt aggaatgacc gcaaaactct tcatgtcacc cttccg
//
LOCUS       DAOSOF010000063        10053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6696_length_10053_cov_4.754751, whole genome shotgun sequence.
ACCESSION   DAOSOF010000063 DAOSOF010000000
VERSION     DAOSOF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_10053_cov_4.754751"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLM68_04895"
     CDS             <1..899
                     /locus_tag="PLM68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT54735.1"
                     /translation="RSNFVYRIKNTNTFVSLGLKENYKTTELHVDVNLVENDRLNLEA
                     FRQWNPEYKDARFILENGEYICGWTVEKMSKSMFNVVNPDVICQRYGADTLRMYEMFL
                     GPLEQSKPWDTNGIDGVHRFLKKFWRLFFDKNGELAVTNNPPVEKELKILHNLIRKVE
                     DDIESFSFNTTISAFMIGVNQLTDLDCHKRGILEPFVILISPYAPHIAEELWSLLGNK
                     PSVANAAFPVFNQEFIKELIFEYPVSFNGKLRFKKTLDINMNKDEIERLVREDERTIK
                     YSGGEKIRKIIVVPEKIINIVI"
     gene            896..1801
                     /locus_tag="PLM68_04900"
     CDS             896..1801
                     /locus_tag="PLM68_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT54736.1"
                     /translation="MRNRISFRMGTSIKEYLLITIGVLVYVASWTTFIVPNQLVGGGV
                     TGISTLIYYTTGLPISVSYLTINAILIITGLKTIGWKFGVKTIYGVALAGISFQLMPG
                     LIPADLIKAMALDNGKLVSSLLGAVMSGAGVGMVIAQGGSTGGTDILAQIIGKYRNIS
                     PGRIMMYCDVVIIGSLFFISDEPTAGLKLAAVLYGYFIVGLASYTVDTILSGTKRSVQ
                     YFIFSSRHQEIADRISKELQRGVTVISSQGWYTKSEHKVLLVIVRRTETNAVAKIIKE
                     VDRDAFMSMASVSGVYGAGFEVLRT"
     gene            complement(1802..3097)
                     /locus_tag="PLM68_04905"
     CDS             complement(1802..3097)
                     /locus_tag="PLM68_04905"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOT54737.1"
                     /translation="MKILAILITMILGLATVQAQDSFESALRTHVKELTANKYMGRKA
                     GSKGESDAAAYISRQFSGKGLELLYPEGYQDFSFIDEETKDTLYSCNVVAVIPGYDPL
                     LREEYIVIGAHYDNLGTYTMRVNGKDSLCIYPGADANASGVAALIELAGAARSQHFMF
                     KRSLLFVAFGAGQKGGLGSWYFINRAFPASAVKMMINLDRVGGASLENLPAAYCGQPH
                     YEMERLLTRLTYSPFMPKVQVYGTDYFMSDHQAFLEQRIPSCLFTTGLQPHHQTLRDT
                     PDKPDYERMDAFCQYISLFIMEAANDPGDLFRKESAPLQATGDDPVYAYNEVDIPPKF
                     MNGDIQSFVDRWLYKYRRYPREAVIQGISGRVMVSFVIEKNGAVSLVEVTRPVHPLLD
                     EEAVRVIAASPKWKPGEKKGEKVRVKISIPVYFELAPTR"
     gene            complement(3094..4203)
                     /gene="gcvT"
                     /locus_tag="PLM68_04910"
     CDS             complement(3094..4203)
                     /gene="gcvT"
                     /locus_tag="PLM68_04910"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT54738.1"
                     /translation="MKQTVFTRFHEELGAKMVPFAGFLMPVEYQGINAEHIQVRTSAG
                     VFDVSHMGEIWVKGPNALPFIQYVTSNDASLLQDGKVQYTCFPNGRGGIVDDLLVYRV
                     DEQTYLLVVNAANTAKDWEHLCSHAPRFGITPGKELYDASSEICQLAVQGPNAMKIVQ
                     KICDAPVMDMEYYTFRKCTVAGIPQAILSITGYTGAGGCEIYVANRDGEQLWKALFEA
                     GKEFGLMAAGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKFTEAKGDFIDRA
                     FLQEQKSRGVSRKLVGFKMIDKGIPRHEYSVCAADGKEIGRVTSGTMSPMLKIGIGMA
                     YVETAYAGLETELYIKIRDRLLKAEVVRPPFYKKQ"
     gene            complement(4207..4683)
                     /locus_tag="PLM68_04915"
     CDS             complement(4207..4683)
                     /locus_tag="PLM68_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54739.1"
                     /translation="MKKIFPLLIGIMLLGSLNSCDWIRSRLGMPTSVELKQKKEQIIK
                     DSLDKLAREQEAARLEQFRLDSLARIEAEKEALKRYHVVLGSFIMDNNAKRMMETLTR
                     LGYQPKRLEFRNGYSVISAYQSNSLSEAYNKMYTMFALDITPYDVWVYDTNQNLHF"
     gene            4771..6015
                     /locus_tag="PLM68_04920"
     CDS             4771..6015
                     /locus_tag="PLM68_04920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT54740.1"
                     /translation="MANKIRTACILVLVFLPLSVFPQDVARVRFCFAGDLMQHGTQIR
                     SALQPDGSYRYDTCFSYIRDRFRNADFAVLNMETTYSGPPYTGYPSFSSPDDLAISTK
                     KAGVNVFLTANNHILDKGVEGARRTVSLYDSLGIMHTGTIRPWIILEKNGIKTALLNY
                     TYTSNNNSGNESFMLNLTDTSLIRRHIGEVRDNGADCIICCMHWGVEYQLHSAPEQRL
                     LAHWLRKAGVTAVIGSHPHVPQSIEVVNGEDGKPAFLVAYSLGNFISNQPEPFTRMGL
                     ILNFELVITQTGPCIESLWYEWIWTWCPVRDGKKTYLVVPVSDNRLYREIATEPSDTL
                     LIVKTIKTLRNFMRETSPAIYERIRYPAYERKNLYFGIHPAIRPLWTRQPVDSPVKGK
                     FSTVKDHSQGRTTQGSGTTGNN"
     gene            5924..6907
                     /locus_tag="PLM68_04925"
     CDS             5924..6907
                     /locus_tag="PLM68_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT54741.1"
                     /translation="MIPLLKENFPQLKIIARGEQLKVQGPPEIIDRFEEKLESVVALY
                     EKRQHLSRESLFSILSGAVQADAGEKKETDNILVYGNNGALIRARTKNQELLVKSYEE
                     HDLVFAEGPAGTGKTYTAIALAVRAWKNKEVRRIILTRPAVEAGEQLGFLPGDMKEKL
                     DPYLQPLYDALRDMIPAKRLESLMEEGVVQIAPLAYMRGRTLDHACVILDEAQNTSLS
                     QLKMFLTRMGKDAKFIITGDTSQIDLVKKSDSGLSRCMELLRDIRGIAFVRFDSGDII
                     RHPLVKKIVAAFATLPAAGSPAASSKTSSKAAKTATTEASASESATKTSGS"
     gene            complement(6794..7621)
                     /locus_tag="PLM68_04930"
     CDS             complement(6794..7621)
                     /locus_tag="PLM68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT54742.1"
                     /translation="MKRSANILIIIVCVLGFSFSLQAQVPERPEVQRLVNDFAGLFTS
                     EETEMLEAELVDIDNTTSNQICVVTVADLGGEDINMAAYQVLVNWGVGTARNNNGVVL
                     LVEHTQGIPGGRVAISVGYGLEGVLTDALSKRIITYDMIPLFRQDNYFEGVRAGVRSI
                     REVATGEYNTARENRDDTPAGVVAVLTVFLILLFFILIIYIANKRKGPTNLGGSNRKG
                     PSMLELMILANLLGGRSRGGSGTGGFGGGFGGGGFGGGGFGGFGGGLGGGGGASGSW"
     gene            complement(7618..8049)
                     /locus_tag="PLM68_04935"
     CDS             complement(7618..8049)
                     /locus_tag="PLM68_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT54743.1"
                     /translation="MQHLFSSRDKKSIVETITRAELKTSGEIRVHIETYCPSSSLERA
                     VFVFNQLKMYKTRDRNSVLIYLASESRKFAVIGDTGINAVVPEGFWDSVKEIMKESFV
                     TGRFTEGICAAVEEIGVKLTRYFPYQPDDVNELPNDISFGS"
     gene            complement(8056..9111)
                     /locus_tag="PLM68_04940"
     CDS             complement(8056..9111)
                     /locus_tag="PLM68_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOT54744.1"
                     /translation="MESKKSNTFFLLVSIFIITLTAACKKDDPDTLYMFGALTFNLPS
                     YTFPGDSILIEASGITKPEEDSIVYTFQGKHFTPDSIVARSAWFTMPDSLGTYTLTLM
                     ASAEGYTKSSTSRSTTVIHDVFSVMMEGFIPSGDSIIDPRDNNIYYFKRFGNLDWFIQ
                     NLNWNEVGMTYSNIPALGFMMGGLYSWNQALSGDNTPVSPPLPASGLGNGPQGVCPQG
                     WSIPTAEDWADLATAVSGTAMGFFDPWNGLGEIFALDARVNGNKVWMYHPDNEKTNTT
                     GWNALPAGYAILSGDTFSGYGQTSFWWAACADGNQGYYRLIHYAVDRFSCAATDKSSV
                     FASVRCVRKAGDNSQSD"
     gene            9160..>10053
                     /locus_tag="PLM68_04945"
     CDS             9160..>10053
                     /locus_tag="PLM68_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT54745.1"
                     /translation="MIQKAFSIIILLILFLFTPACNQLPRYECLEGSALGTTYAITFR
                     IPPDAPSGFTANAEQKLTAAFEMINNSMSIYNNHSLIHKINHNISQETDSLFRIVYQR
                     SLEITTLTGGAFDISAAPLFDLWGFGFENKDTIRKESLAEVKEYTGMDKFRLEGTLLY
                     KADPRCRLSMNAIAKGFTCDLIAGELSAFGIEDMLVEIGGEIACKGLNPKKKPWRIGI
                     DNPVDGNIHPGEMIQAVIIMTDKGLATSGNYRNYYIENGQKFAHIIDPVSGLPVNHNL
                     LSATVIASDCMTADAFATAFMV"
BASE COUNT         2685 a         2469 c         2329 g         2570 t
ORIGIN      
        1 gacgttccaa tttcgtttac cgcatcaaaa acacaaacac atttgtctcc ctgggcctga
       61 aagaaaatta caagacaaca gagcttcatg tggatgtcaa cctggttgag aatgaccgtc
      121 tgaacctgga agcgttcagg cagtggaatc ccgaatataa agatgcccgg ttcatactgg
      181 aaaacggaga atatatctgc ggatggactg ttgaaaaaat gagtaaatcc atgttcaatg
      241 ttgtcaaccc ggatgtcatc tgtcagcgtt acggggcaga caccctgcgg atgtacgaaa
      301 tgttcctggg tcccctggag caatccaaac cctgggatac taatggtatt gacggcgtac
      361 acagattcct gaagaaattc tggaggttat tctttgacaa aaacggggaa ctggccgtaa
      421 ccaataaccc cccagtggaa aaagagttga agatcctgca taacctcatc cggaaggtgg
      481 aagatgacat tgaaagtttt tccttcaata ccaccataag cgcatttatg attggggtaa
      541 atcagctgac cgatctggat tgtcataaaa gggggatcct tgaaccgttt gttatcctga
      601 tatctcctta cgcaccgcac atagccgaag aattgtggtc gcttctgggt aacaaaccat
      661 ccgtggcaaa tgcagccttc cccgtgttca atcaggaatt catcaaagag ctgattttcg
      721 agtatcctgt ttcattcaat ggcaagctcc ggttcaaaaa aaccctggac atcaacatga
      781 ataaagatga aattgaacgc cttgtcaggg aagacgaaag gacaatcaaa tacagcgggg
      841 gcgaaaaaat ccgaaaaatc atagttgttc ctgaaaaaat cataaacatt gtgatatgag
      901 aaacagaata tcattcagga tgggtacaag cattaaggaa taccttctca ttaccattgg
      961 agtgcttgtc tatgtggcat cctggactac tttcattgtt cccaatcaac tggtgggagg
     1021 aggtgttaca ggtatttcca cactcatcta ctacacgacc ggattgccaa taagtgtttc
     1081 ctatctgacc atcaatgcca tcctgataat cacagggctc aaaaccatcg gctggaaatt
     1141 cggcgtaaaa accatttacg gtgtggccct ggcggggatt tcttttcagc tcatgccagg
     1201 tctcatcccg gcagatctta tcaaggcgat ggccctcgat aacggaaaac ttgtttcttc
     1261 actgcttgga gctgttatgt ccggagccgg agtaggtatg gtcattgcac agggaggcag
     1321 tacgggaggc acggacatac tggcgcagat catcggaaaa tacagaaaca tttcaccagg
     1381 ccggatcatg atgtactgtg atgtggtgat cataggctct ttgttcttta tctcggacga
     1441 gcccacggct ggattgaaac ttgctgctgt attatacggt tattttattg ttggcctggc
     1501 tagttatact gttgatacca tattatcggg gacaaaacgc tctgtgcagt atttcatatt
     1561 ttcttcccgg catcaggaaa ttgcagaccg tatatcaaaa gagctccaaa gaggagttac
     1621 cgtcataagc tcacagggat ggtacaccaa gtctgaacat aaggtgttgc tggtgatagt
     1681 acgaaggact gaaacaaatg cggttgcgaa aatcatcaag gaagtcgaca gggatgcttt
     1741 catgtccatg gcaagtgttt ccggtgtgta cggtgccggt tttgaagttc tcaggacgta
     1801 atcagcgtgt tggtgccaac tcaaagtaga caggaatcga tatcttcaca cgtacttttt
     1861 cacctttctt ttctccaggt ttccattttg gtgaagcagc aatgacgcgg acagcctcct
     1921 cgtccagcag gggatgtacc ggcctggtaa cctcaaccag tgatactgcg ccgtttttct
     1981 caataacaaa tgagaccatt acacgaccac tgataccctg gatcactgct tcccgcggat
     2041 aacgacggta cttgtatagc catctgtcca caaatgattg tatatcacca ttcatgaatt
     2101 ttggaggaat atccacttca ttgtatgcat acacgggatc gtccccggta gcctgcaagg
     2161 gagcagactc cttccggaaa agatctcccg ggtcattggc cgcttccata atgaacaagg
     2221 atatgtactg gcaaaaagca tccatccttt catagtccgg cttatcggga gtgtcgcgca
     2281 aggtctggtg gtgtggttgc aatcccgtag tgaacaggca ggaaggtatc ctctgctcca
     2341 gaaaagcctg gtgatcagac atgaagtaat cggtcccata aacctgtact tttggcatga
     2401 aaggactgta tgtcaggcgg gtaagaaggc gttccatttc atagtgaggc tgcccgcaat
     2461 aagctgctgg gagattctca agagaggcgc cgcccaccct gtccaggttg atcatcattt
     2521 taacagcaga agcaggaaat gccctgttaa taaaatacca ggacccaagc ccccctttct
     2581 ggccggcccc gaaagccacg aacaaaaggc ttctcttgaa catgaaatgc tgactccggg
     2641 cagcccctgc cagttcaatc aaagcagcaa ctcccgaggc attggcatcg gctccgggat
     2701 aaatgcacag cgagtctttc ccgtttacgc gcatggtata ggtccccagg ttatcgtagt
     2761 gggctccaat gactatatat tcttccctca gcagaggatc atatccggga atgacagcca
     2821 ccacattgca ggaatacagt gtatcctttg tctcctcgtc aataaaggag aaatcctggt
     2881 aaccttcggg atagagcaat tcaagaccct tgccggaaaa ttgacgggaa atataggcag
     2941 ctgcgtcgga ctcccccttc gagccggcct tccttcccat gtatttattg gcagtcagct
     3001 cttttacgtg cgttctgagt gcactttcaa aagaatcctg agcctgcacg gtggcaagac
     3061 ccaggatcat tgtaatgagg atagcaagaa ttttcattgt tttttataaa acggcgggcg
     3121 gaccacctcg gccttcaaaa ggcggtctcg tatttttatg tacaactctg tttccaaccc
     3181 ggcataggcc gtttctacat atgccattcc tatgccaatc tttaacatgg gagacatggt
     3241 accggatgtt acacggccaa tctcctttcc atcagctgca caaaccgaat actcatgcct
     3301 tggtattccc ttgtctatca ttttgaatcc caccagttta cgtgaaacac ccctggattt
     3361 ttgttcctgc aggaaggccc tgtcgatgaa atctcctttt gcttcagtaa attttgttat
     3421 ccaacccaga ccggcttcca gcggagaggt ggtgtcatct atgtcatttc cataaagaca
     3481 gaaacccatt tcaaggcgta atgtatcgcg ggctcccaat cctgccgcca tcagtccgaa
     3541 ctctttcccg gcttcaaaca atgccttcca aagttgctcc ccgtcgcggt tggccacgta
     3601 tatctcacat cccccggctc cggtataacc cgtgatggac aggatcgcct gcggaatccc
     3661 tgcaacggtg catttccgga atgtatagta ttccatatcc atgacagggg catcacagat
     3721 cttttggact atcttcatgg cattcggtcc ctgaacggcc agctggcata tttctgaaga
     3781 ggcatcatac agttcctttc cgggagtaat cccgaagcgg ggtgcatggg agcagaggtg
     3841 ttcccagtct tttgcggtat tggcagcatt gacgaccaat aaataggtct gttcgtctac
     3901 cctgtatacc agcaaatcgt ccactatgcc tccccttcca ttgggaaaac aggtgtattg
     3961 cactttcccg tcctgaagca atgaggcgtc gtttgatgtt acatattgaa taaatggcag
     4021 cgcattgggt cctttgaccc atatctcccc catatggctg acgtcaaaga caccggcact
     4081 ggtgcgaacc tggatatgtt ccgcattaat tccctggtat tcaacaggca ttaaaaatcc
     4141 ggcaaagggc accattttgg cccccagctc ttcgtggaac cgggtaaaaa ctgtttgttt
     4201 catagatcag aagtgtaggt tttggttggt atcatatacc cagacatcgt aaggagtgat
     4261 gtccagggca aacattgtgt acatcttgtt ataagcctct gaaagtgaat ttgactggta
     4321 ggccgaaata accgaataac cattcctgaa ttccagcctt ttgggttgat aacccaagcg
     4381 ggtcaaagtt tccatcattc ttttggcatt gttgtccata atgaagcttc ccaggaccac
     4441 atggtatctt ttcaaagcct ctttctccgc ttcgatcctg gccagcgaat ccagccggaa
     4501 ctgttccagt ctggcagcct cctgctccct tgcgagctta tcaagtgaat ccttaatgat
     4561 ttgttccttc ttctgcttta actctacaga ggtaggcatt cctaatctgg aacgtatcca
     4621 atcgcaggaa ttcaaactgc ccagcagcat gattccaatg agcaacggga atattttctt
     4681 cataataaaa tcaatagata gtttttgtat gacaaatata catgaaaaaa acgggaacaa
     4741 tgaaaaaagg ggtaacttgc aacaggtatt atggcaaata agatccggac agcatgtatc
     4801 cttgttttgg ttttcctgcc gctaagcgtt tttccgcaag atgtggcccg ggtccgtttc
     4861 tgttttgcag gagacttgat gcagcacggt acacaaatcc ggtcagccct tcaacctgac
     4921 ggttcatacc ggtacgacac ttgtttttca tatatcaggg accggttccg gaatgcagat
     4981 tttgcagttt tgaacatgga gacgacttac agcggccccc cctatacggg gtatccgtct
     5041 ttttcttctc ccgatgatct ggccatatcc accaaaaaag ccggggtcaa tgtttttctt
     5101 actgccaaca accatatcct tgacaaggga gttgagggtg cccgcagaac cgtgtcgttg
     5161 tacgattccc tgggcataat gcacacgggg accatacgtc cctggatcat attggaaaaa
     5221 aacgggatca agacagccct gctgaactat acttatacaa gcaataacaa ttcaggcaac
     5281 gagtccttca tgctcaacct gacagatacc tctctcataa ggaggcatat aggcgaagtc
     5341 cgggataatg gtgcagattg cattatctgc tgcatgcact ggggagtgga gtaccagttg
     5401 cactctgctc ccgaacagcg tctgctggct cactggctca ggaaggccgg agtaaccgct
     5461 gtgattggat ctcacccgca cgttccccaa agtatagagg ttgtgaacgg agaagacgga
     5521 aagcccgcct tcctggttgc ttactctctg ggaaacttta tttccaacca acccgagcca
     5581 tttaccagga tgggtttgat cctgaatttt gaactggtga ttacccagac aggcccctgc
     5641 attgaatctc tctggtatga atggatctgg acctggtgtc cggtcaggga cggaaaaaaa
     5701 acgtatcttg ttgtaccggt gtctgataac cgcctgtaca gggaaattgc tacagagccg
     5761 tccgatacgc tgctgattgt taaaactata aaaacacttc gtaactttat gcgcgaaaca
     5821 tctcccgcca tttatgaacg tatcagatat cccgcttatg aacgaaaaaa tctttatttt
     5881 ggaatccatc ccgccatccg acctttatgg acccgacagc ccgttgattc ccctgttaaa
     5941 ggaaaatttt ccacagttaa agatcatagc caggggagaa caactcaagg ttcagggacc
     6001 accggaaata attgaccggt ttgaggaaaa gctggaaagc gttgtggctt tatatgaaaa
     6061 aagacaacac ctgtcccgtg aaagcctctt ttccatatta agcggcgcag tgcaggcaga
     6121 tgccggtgaa aagaaagaaa cggacaacat tctggtttac gggaataatg gggccctcat
     6181 acgtgccagg acaaaaaacc aggaactcct tgtaaaatcc tatgaagaac acgatctggt
     6241 ctttgcggaa ggccctgccg gtaccggaaa aacctacacg gcaattgccc ttgccgtacg
     6301 tgcttggaaa aataaggaag tccgcaggat aatcctgacc cgtccggccg tggaggcggg
     6361 agagcagctg ggctttcttc cgggagacat gaaggagaaa ctcgatcctt acctccagcc
     6421 tttatacgac gcacttcggg acatgatacc agctaaaaga ctggaatcgc ttatggagga
     6481 aggtgtcgtg caaatagccc cgctggcgta catgagggga agaacgctgg accacgcatg
     6541 tgtgatcctg gatgaagctc agaacacaag cctgtcgcaa ctcaaaatgt tcctcaccag
     6601 gatgggaaaa gacgcaaaat ttatcataac aggagatacc agccagatag atctggtcaa
     6661 aaagagtgac agcggattgt cccgctgcat ggagctgctg cgtgatatca ggggtatcgc
     6721 tttcgtccgt ttcgattcgg gtgatatcat aagacacccg ctggtaaaaa agatagtggc
     6781 tgctttcgcc acactaccag ctgccggaag ccccgccgcc tcctccaaga cctcctccaa
     6841 agccgccaaa accgccacca ccgaagcctc cgcctccgaa tccgccacca aaacctccgg
     6901 ttcctgaacc tcccctgctt cttcctccca gaagattggc taagatcatc aattccagca
     6961 tgctgggtcc tttcctgttg cttccgccca ggttggtcgg gccttttctt ttattggcta
     7021 tataaatgat caggataaag aaaagcagga tcaagaatac tgtcagtact gccaccaccc
     7081 cggcaggtgt gtcatccctg ttttccctgg ctgtattgta ttcacctgtg gcaacctccc
     7141 tgattgacct cacacctgcc ctgactcctt caaaatagtt atcttgcctg aacaggggga
     7201 tcatgtcata ggtaatgatc cttttggata acgcatcggt cagcacacct tccagaccgt
     7261 agccgacaga aatcgctacg cgtccaccgg ggattccctg tgtgtgttcc accagcaaga
     7321 caaccccgtt gttgttcctg gccgtcccaa ctccccagtt taccaacacc tggtaagctg
     7381 ccatattgat atcctccccg ccaagatcgg caaccgtaac cacgcatatc tgattggatg
     7441 tggtgttatc gatatcgacg agttccgcct caagcatttc cgtttcttca gaggtgaaca
     7501 aaccggcaaa atcattcacc agacgctgaa cctcgggacg ctcaggcacc tgagcctgca
     7561 gtgaaaaaga gaaccccaga acacagacta tgattatcag tatgttagcg gatcttttca
     7621 tgatccaaat gatatatcgt tgggtaattc attgacatcg tcaggttgat agggaaaata
     7681 ccgggtcagt ttgacaccta tctcttccac tgcggcacaa atgccttcag taaagcgacc
     7741 cgtgacaaag gattctttca tgatctcttt cacggaatcc cagaaacctt ccggaacaac
     7801 ggcattgatg cccgtatcgc ctatgaccgc gaatttcctg gattcgctgg ccagatagat
     7861 aagtacagag ttacggtccc tggttttata catcttcagc tgattgaaaa cgaatactgc
     7921 acgctccagc gaagaggaag gacagtatgt ctcaatgtgt accctgattt ctccggaagt
     7981 tttcaattcg gcccgggtta tcgtttcgac gatcgatttc ttatccctgg atgaaaatag
     8041 atgttgcatc tgatctcagt ctgattggga gttatcacct gcttttcgca cgcatcttac
     8101 agatgcaaat accgatgact tatcggtagc tgcacaggaa aacctgtcta ccgcataatg
     8161 tatcaaacgg tagtagccct ggttgccatc agcacaggca gcccaccaga atgaagtctg
     8221 accatacccg gaaaaagtat ccccggaaag gatcgcatat cctgccggaa gggcattcca
     8281 gcctgttgta tttgtctttt cattatcggg gtggtacatc cagaccttgt ttccgttgac
     8341 ccgtgcatca agtgcaaaaa tctcccccaa accgttccag ggatcaaaaa agcccatggc
     8401 tgtaccactg actgccgttg ccagatctgc ccaatcctcg gccgtgggga tgctccaacc
     8461 ctgggggcag acaccctgtg ggccgtttcc caggccactg gccggcaggg gaggtgacac
     8521 cggtgtgtta tctccggaaa gagcctgatt ccagctatat aagcctccca tcatgaatcc
     8581 cagcgccggg atgttggaat aggtcatgcc cacttcattc cagttcagat tttgaataaa
     8641 ccagtccagg tttccaaaac gcttgaaata atagatattg ttgtcccttg gatcaattat
     8701 tgaatccccg gaaggtatga aaccttccat catgacagaa aaaacatcat gtatgacggt
     8761 agtggaacgg gaagtggagc tttttgtgta gccttcagcc gatgccatca gtgtgagggt
     8821 gtaggtaccc aggctgtccg gcatggtaaa ccaggctgat cgggcaacta tcgaatcggg
     8881 cgtaaaatgt tttccctgaa aagtataaac tatcgaatct tcctccggct ttgtaattcc
     8941 cgaggcttca atcaggatgg aatcgcccgg aaaggtatag gatggcagat tgaaagtcag
     9001 ggctccgaac atgtacaggg tgtccggatc gtcctttttg catgctgctg taagtgtaat
     9061 gatgaaaatg gataccagga gaaaaaatgt atttgatttt ttactttcca tatcaattat
     9121 aatctgcaaa tgtaggtaaa aatttgtagc tttgtgtaaa tgatacaaaa agcattttct
     9181 attattatcc tgctcattct gttccttttt actcccgcct gcaaccagtt gccccgttac
     9241 gagtgtcttg aagggagtgc cctgggaaca acgtatgcca tcacgttcag gataccgccc
     9301 gatgcaccgt ccggttttac cgccaatgca gagcaaaaac tgactgctgc ctttgaaatg
     9361 atcaacaatt caatgtctat atacaacaat cattccttaa tccataaaat caatcataat
     9421 atttcacagg agacagacag cctgttcaga atcgtatacc aaagatcttt ggagatcaca
     9481 accctgacag gaggggcctt tgatatatcg gcggctcctt tatttgattt atggggattt
     9541 ggatttgaaa acaaggatac catcagaaag gaaagtctgg ctgaggtcaa agaatatacg
     9601 ggcatggata aattccgcct tgaagggacg ctcctttata aggcagatcc gcggtgcaga
     9661 ctgagtatga atgccatagc caaaggattc acctgtgatt tgatagccgg tgagttatca
     9721 gcattcggta tagaggatat gctggtggag ataggcggtg agattgcctg caagggcctg
     9781 aatcctaaaa aaaagccgtg gcgcataggt atagacaatc ccgttgacgg gaacattcat
     9841 ccgggtgaaa tgatccaggc tgtgataatc atgacagaca aaggtctggc tacttcaggt
     9901 aattacagga attattacat agaaaacggt caaaaattcg cacatatcat agatcccgta
     9961 tccggccttc ctgtgaatca caacctgttg agtgcaactg tcattgccag tgactgcatg
    10021 acagcagacg cgttcgccac cgcttttatg gtt
//
LOCUS       DAOSOF010000064         9990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6744_length_9990_cov_5.180876, whole genome shotgun sequence.
ACCESSION   DAOSOF010000064 DAOSOF010000000
VERSION     DAOSOF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6744_length_9990_cov_5.180876"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PLM68_04950"
     CDS             <1..1278
                     /locus_tag="PLM68_04950"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT54746.1"
                     /translation="EQTLRDLLTWENIMGVTRHDMDALLDRPVFWLFFAGKAQNSSVF
                     QTQVVCEKEEVPEDHLVFLTRKLYTDSVKAELEKLHGGYSATTFRVRSFDHQGRVLRP
                     TVLKTGDRPMIQREAERCKQYAMPYILNNSAMILGTSFYCQMGALRYNFVGIGGEDTE
                     LKWLTHYFHSWPVDDLVSLFDKIFLKILKPWYGQAVPEVIYPYADHDPTKTFFPHVFR
                     QAEEELGIPEKNRSFTIGGSEKNFLNPYHYLHEEYPRRRKQGMDYYSAICHGDLNMQN
                     ILLDKDMNVYLIDFSETRPRSAISDFARLEAIFMIESFPMENENDFLQVLEFLMDFYE
                     KPVTLDKKFRKAWPGALPGLMDRNMTLTMKMREYALAAARGNKTFTPYAVALLEWILP
                     IVCYSSATIWQKRLSACVASLLCETIESGKEAQ"
     gene            1316..1846
                     /locus_tag="PLM68_04955"
     CDS             1316..1846
                     /locus_tag="PLM68_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOT54747.1"
                     /translation="MLTSLFSNKGQQNTGKIVEPAEDFYALSFKTIRGETFSFEQLRG
                     KKVLLVNTASKCGFTPQFKELEALNKEYAPNLVILGFPSNDFMKQDPGTNEEILEFCE
                     INYGVTFQMMEKSSVKGKDQNPVFRWLSDKSKNGWNTSAPGWNFSKYLVDEKGRLIGF
                     WPSKVKPNDPQILEML"
     gene            complement(1857..2657)
                     /locus_tag="PLM68_04960"
     CDS             complement(1857..2657)
                     /locus_tag="PLM68_04960"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT54748.1"
                     /translation="MRKHYIIILCLIAAGCGNISNKQICWDRGQWLDLTWDFEKSTVY
                     WPTNVTFIHDTVTFGINDKGYFYSSFLYAAEEHGGTHFDAPLHFAENGRSIEQILPEE
                     FTGEGICVDVSDKVLADRDYLITSEDFLGWEKTHGTIPAQTIVLLRTGFGQYWNDPLQ
                     YTGTLKTGSEGVGELHFPGLDPEAALWLIEERNVKAVGIDTPSIDYGQSADFLTHRTL
                     FSHNVTAYENVAHLQSVPARNFYVVAAPMKIKGGSGAPLRLLVWIPEV"
     gene            2731..4572
                     /locus_tag="PLM68_04965"
     CDS             2731..4572
                     /locus_tag="PLM68_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT54749.1"
                     /translation="MKKTTMLTAALLGCALQASARPYEKGPYTVTRLEEDVYNIVDAN
                     RQNPAGMHNNKTGEVTGMNNSSDMYLVLGTEKALLIDLSNNIDWYEDPAGRLQEIVYD
                     LARSRQLVITLTHRHGDHLGMLPAFRDDSLVRFWVPENDFSGSELFPDQRTVFFKEKE
                     SLDLGGGVIVDSFSLPGHTPGSTLFFLRGRHLVFTGDALGSGNGLWLLNEESFGQLSA
                     SFGSLMKHILDPSNGISHARLVLYTGHSWQKGTSGPLGSNYLEDMQVLIGQIGSGTAL
                     TEPYQTFLPFLNANFRYQSATITWNREAAERFVEEKRFPPERDFTGQGPTHRGNNFEL
                     IKLLDSHNFTLDDSPVGDMEYYLYDPVAHGADPGKKYPLIVMLHGASNGMEGVMCAAY
                     TDFVVYAGEEYQQKIGGAYILFPKANEYVQMEGDNQVILGTWMTRDATQEGSVYTSVL
                     AALLEDVISAHNIDEERVVIGGTSAGGYMVWRFLAARPDLVKGAFLVAPADNPSEEEL
                     KTYEKHGIHIWVIHGKKDEICPFGVFTGPVRNMLEATKNVRVSALETVRYGDKGIVRL
                     NVRGTEMGQHLPLFCVGSDMIYDDGTPYDPRYPEGFTGWLNMVFGND"
     gene            4565..5212
                     /locus_tag="PLM68_04970"
     CDS             4565..5212
                     /locus_tag="PLM68_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT54750.1"
                     /translation="MIDTVIFDLGGVIVNIDRDCSVKAFQALGLKDAGTYLDPYHQSG
                     IFLELEDGRLDADEFCERLGQLCGRPVSFLQAQEAWMGFISGVPGERLAYVESLRKDY
                     QVCLLSNTNPFIMEWARSARFTPQGRALDVFFDQLFISYQMKCVKPHRQIFRQVLDTL
                     GAKPEHILFVDDGPANIETAKDMGFLTLMPVNGTNWIPDVQKVLANAVTRRNRGR"
     gene            complement(5164..6438)
                     /locus_tag="PLM68_04975"
     CDS             complement(5164..6438)
                     /locus_tag="PLM68_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT54751.1"
                     /translation="MRLRYFIITGSLLVSFTCIHAQDTTYYKEVIRELSSKKYEGRSD
                     FKKGDRKAAKYIARQFMQIRGVEPSVEGGYLQPFSYNVNVFHKKMEMSVDGVTLEPGK
                     DFIVREFSPSQKGIFSLKYIGVQDHDPARLIPMLESGDFRNSYVVLQYDLVYKYMGIA
                     PFELYKMPVAGLIMVQDKVPSFFKSRSGTVQPIPVIWAGPGFPGDAKQIRVRFESRMI
                     EGYTSSNVIAHIPGSSRADSCIVFVAHYDHLGHFGKEVYYPGAHDNASGVAFILTLAR
                     HYAQPENRPELTYVFIAVAAEENNLLGSTHYTKNPVFPLERTLQVIDPDMVADNGERL
                     FVETGAEGEENLKRFLHINDSLGLFTIEQHPLSNDSDHYPFAMKEVPALYMMVDGDAY
                     DVYHTPLDNSGNMYINRYLPLFRLVTAFASTF"
     gene            6474..7544
                     /locus_tag="PLM68_04980"
     CDS             6474..7544
                     /locus_tag="PLM68_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT54752.1"
                     /translation="MKRLALIVLAIAAFACTEKNIRTISPEADVVMARLQQAAGNNQF
                     FFGQEDFPFYGHSWEYEEGRSDVKEVCGDYPAVLGCDLGHIELGDDKNLDGVPFEIMR
                     KAITDHYMAGGIVTISWHARNPLTSSNAWDVSDNTVVASILEGGSHYDLFQTWLDKVA
                     VFLDSLRMEDGTRIPVIFRPWHEHTGSWFWWGRKLCSSQEYIALWKMTVDYMRNKGLD
                     NLIIAYSPNYGGLDREAYMERYPGDDYVDLLGLDAYGDGQEFLDVLRNELTMVAQLGK
                     EHKKAIALTECGMGGLGNPLWFTQILLKATEGLPVTYALVWRNAPDYLMPMHIFAPYP
                     GHASAKDFITFYQDPRTLFLND"
     gene            7555..8247
                     /locus_tag="PLM68_04985"
     CDS             7555..8247
                     /locus_tag="PLM68_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOT54753.1"
                     /translation="MKKLYVILLILFLTSSVPAISQNVQLHYRTGQWLYPDTLGKDAR
                     ILTTVEMFRMDPWGDTFLFVDMTYTPQGVNYAYWEIARNLKFWDAPVAVHLEYNGGLL
                     GSILFNHSWLAGVNYGIASKDGSKSFSVSAMYKYIQGLARPSNFQLTAIWNMELARGK
                     CTFSGFVDWWRQGDTYILLSQPQFWVNLNAFEGISDSFCLSVGTEVELYNNLFHKGFY
                     VIPSLAVKWTFR"
     gene            complement(8256..9644)
                     /locus_tag="PLM68_04990"
     CDS             complement(8256..9644)
                     /locus_tag="PLM68_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOT54754.1"
                     /translation="MFSKEIYTQRRQTLKSKLNKGIGLFMGNREAPCNYSGNTYHFRQ
                     DSTFLYYFGLDEPDLAAVIDFETGKEVLFADDVSLDDIVWTGPLPAMQEKAEKIGLSH
                     TSPRKNLATYIREEKRKGRTVHFLPPYRSDNMIELMDMLDIHPNELKEQASEELIRAI
                     VGMRMKKQPEEIEQIDRACNLGYAMHFTAMKLAKPGMIEQELAGIMEGIAVSGGYMPS
                     FPIILTQNGEVMHGHAHDQILTEGRLLLMDAGAELQSHYCSDFTRTIPVGGVFKGIAA
                     DLYNLVLKTNNHCIGMCAPGISFRDVHLAAATIITEGLKDLGLMRGDTAEAVAAGAHA
                     AFFPTGLGHNMGLDVHDMEDLGEHYVGYDNTVSRSTQFGLKSLRMARLLEAGNVMTVE
                     PGFYVIPALLEMWKARGHNAAFINFEKCKPLYGFGGIRIEDDVLITTDGHRLLGSKRL
                     PVTEQEIREVMQ"
     gene            9695..>9990
                     /locus_tag="PLM68_04995"
     CDS             9695..>9990
                     /locus_tag="PLM68_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT54755.1"
                     /translation="MLPTSRKELDRLGYGNVDIILFTGDAYVDHPSFGAAVIGRVLQA
                     QGYRVAVVPQPNWRDDLRDFRKLGVPRLFFAVTSGNMDSMVNHYTAARRLRSNDA"
BASE COUNT         2484 a         2397 c         2539 g         2570 t
ORIGIN      
        1 gagcaaaccc tgcgtgactt gttgacctgg gaaaacatca tgggagttac caggcatgat
       61 atggatgcat tgcttgacag gccggtgttc tggttgtttt ttgccggcaa ggcacagaat
      121 tcctccgttt ttcagacaca ggtggtttgt gaaaaagaag aggtcccgga ggatcacctg
      181 gtcttcctga ccaggaaact gtatacagat tcagtgaagg cagaacttga gaagcttcac
      241 ggcggatatt cagcgactac ttttcgtgtc cgctcttttg accaccaggg cagggtcctg
      301 agaccaaccg tactaaaaac cggggaccgg cccatgatac agagagaggc agaacggtgt
      361 aagcagtatg ccatgcctta cattttgaac aacagcgcca tgatcctggg cacgtctttc
      421 tattgccaga tgggtgcact caggtacaat tttgtgggga taggaggaga agacaccgaa
      481 ttgaaatggc taacccacta ctttcacagc tggcctgtag atgatcttgt ttctctattt
      541 gataagattt tcctgaaaat actcaaaccc tggtacggac aggccgtacc ggaagtaatc
      601 tatccatacg cagaccacga tcccacaaaa accttttttc ctcacgtgtt caggcaggcc
      661 gaagaggagt tagggattcc ggagaagaac cgctccttca ccataggtgg ttcggaaaag
      721 aactttttga atccatacca ttatttacat gaggagtatc ccagaagacg gaaacaggga
      781 atggattatt attctgctat ttgtcatgga gacttgaaca tgcagaatat cctgctggac
      841 aaggacatga atgtttatct catagacttc tcagaaacca ggccgcgttc agctatatcg
      901 gattttgccc gtttggaagc catattcatg attgaatcct ttcccatgga aaacgaaaat
      961 gattttcttc aggtcctgga gttccttatg gatttctacg aaaaaccggt taccctcgat
     1021 aaaaagttca gaaaggcctg gcccggagca ttgccgggat taatggacag gaatatgaca
     1081 ctaaccatga aaatgaggga gtatgcactc gctgctgcac gggggaataa gacattcact
     1141 ccctatgcag tggccctcct tgaatggatc ttgccaattg tctgctattc aagcgccact
     1201 atatggcaaa aacgtctctc tgcctgtgtg gcctcattgc tttgtgaaac aattgagtca
     1261 ggtaaagagg cacaataaat gcgaaaccat gaaaacaacc atcattattt taattatgtt
     1321 aacatcctta ttttcaaaca aagggcaaca aaacacggga aagattgtgg aaccggccga
     1381 ggatttttat gcattatcct tcaagaccat ccggggagaa acattttctt ttgaacaact
     1441 gcgcgggaaa aaggttttgc tggtcaacac ggcttccaaa tgcggcttta caccgcaatt
     1501 caaagaactg gaagccctga acaaggaata tgcaccgaac ctggtcatcc tgggatttcc
     1561 ctccaatgat ttcatgaaac aggatcccgg gaccaatgaa gaaatccttg aattctgcga
     1621 gatcaattac ggggtcacat tccagatgat ggagaaaagc tcggttaaag gtaaagacca
     1681 gaatcctgta tttcgctggc tgtctgataa atccaaaaac ggttggaaca ccagcgctcc
     1741 cggttggaat ttttccaagt acctggtaga tgagaaaggc aggctcatag gcttctggcc
     1801 atccaaggtg aaacccaacg acccgcagat actggaaatg ctttaaggca gacagttcac
     1861 acttcaggta tccagacaag gagccttagc ggtgctccgc ttcctccctt gattttcatg
     1921 ggagcagcca ccacatagaa attacgcgct ggtactgatt gcaggtgcgc cacattttca
     1981 taggcggtga cattgtggct gaacagcgtg cggtgggtaa gaaaatctgc tgactgtccg
     2041 tagtcgatac tcggtgtgtc gataccaaca gccttcacat tcctttcttc tatgagccac
     2101 agggcggcct cgggatccaa cccgggaaaa tgcaattctc cgacaccctc ggaacccgtt
     2161 ttgagggtcc ctgtgtattg cagggggtcg ttccagtatt ggccgaaacc ggtacgcaac
     2221 aacacgatcg tctgtgctgg gattgtgccg tgtgtttttt cccatcccag gaaatcttca
     2281 ctggtgataa ggtaatctct gtcagccaaa accttgtctg atacatcgac acatatacct
     2341 tcgcctgtga attcttccgg aaggatctgt tcaatggacc ttccgttttc cgcgaaatgc
     2401 aatggcgcgt caaaatgggt tcccccgtgc tcttctgccg catataggaa agaggaatag
     2461 aaataacctt tgtcattgat cccgaaagta acggtatcat gaatgaaagt cacgttggtt
     2521 ggccagtaca cagtgctttt ttcaaaatcc catgtcagat ccagccattg gccccggtcc
     2581 cagcagatct gcttatttga aatatttccg caacctgccg cgatcagaca taaaatgata
     2641 atgtaatgtt ttctcatagg aagcctggtt ttattacttc aaatatagca tgatttgaag
     2701 tacatttgta acctggtaaa aaatgttagc atgaagaaga caaccatgtt gacggcagcc
     2761 ttgctcggtt gtgccctgca ggcttctgcc cgtccatacg aaaaggggcc ttataccgtc
     2821 acacggcttg aggaagatgt gtacaacatt gtggatgcca acaggcaaaa tcctgcagga
     2881 atgcataaca acaagacagg agaggtgaca ggcatgaaca acagctccga tatgtacctg
     2941 gtgctgggaa ctgaaaaagc cttgctcatt gacctttcca ataacattga ctggtatgaa
     3001 gaccccgctg gccggctaca agaaattgtc tatgatttgg cccggtcaag gcaactggtc
     3061 ataaccctta cccaccgtca cggggaccac ctggggatgt tgcctgcttt ccgggacgat
     3121 tcgctggtcc gcttttgggt tccggaaaat gatttttcgg gaagcgagct tttccctgac
     3181 caaaggaccg tttttttcaa agagaaggag tccctggatc ttggaggagg ggtgattgtt
     3241 gattcattca gccttccggg tcatacaccc ggatccaccc tcttctttct ccggggccgg
     3301 cacctggtct ttacggggga tgccctgggt tcgggcaatg gtctgtggct cctgaatgaa
     3361 gaaagtttcg gacagttatc tgccagcttc ggttctctga tgaagcatat ccttgacccg
     3421 tccaatggga tttcacatgc aaggctggtt ttgtataccg ggcattcctg gcagaagggc
     3481 acatcgggtc cgcttggatc aaactacctg gaagacatgc aggtactgat agggcaaatc
     3541 ggatccggta ccgccctgac agagccttac cagacattcc ttcccttttt gaatgccaat
     3601 ttcagatacc agtcggccac catcacctgg aacagggagg ccgctgaacg ttttgtggaa
     3661 gaaaagcgtt ttcctcccga aagggatttc acagggcagg ggcccacgca cagggggaac
     3721 aactttgaac tgataaaatt gcttgatagc cataacttta cattggatga ttctccggtg
     3781 ggcgatatgg agtattacct gtacgaccct gtggcacacg gggcggatcc cgggaagaaa
     3841 tacccgctga ttgttatgtt gcatggagct tccaatggca tggaaggggt gatgtgtgcc
     3901 gcttatacag attttgtggt ttacgccggg gaagaatacc agcaaaagat aggaggggcc
     3961 tatatacttt ttcccaaagc caatgaatat gtgcagatgg aaggagataa ccaggttatc
     4021 cttggcactt ggatgaccag ggatgcgaca caggagggct ctgtgtatac ttcggtgctg
     4081 gcggctttgc tcgaagatgt tatctcggcc cataatattg atgaagaacg tgttgtgata
     4141 ggaggcactt cggccggagg atacatggtc tggcgattcc tggctgcccg gcccgacctg
     4201 gtaaaagggg cttttctggt agccccggca gacaatccct cggaagaaga gttgaaaaca
     4261 tatgaaaaac acgggatcca tatctgggtc atccatggga aaaaagatga aatttgtcct
     4321 ttcggggttt ttaccggacc tgtgagaaac atgctggagg caacaaaaaa tgtccgcgtc
     4381 tctgcgctgg agacagtacg ttacggggac aaaggaattg tccggctgaa tgttcgcggg
     4441 acagagatgg gacagcacct gcccttgttt tgcgttgggt ccgatatgat ctatgatgat
     4501 ggtactcctt acgacccgcg ctacccggag ggtttcacag gctggttgaa tatggttttt
     4561 ggcaatgatt gatacagtta tttttgatct gggcggggta attgtcaaca ttgaccggga
     4621 ctgctctgtt aaggcttttc aagccctggg actgaaggat gccggtactt acctggatcc
     4681 ataccaccag agcggcattt tccttgaact ggaagacggc aggctggatg ctgatgaatt
     4741 ttgtgaacgt cttggccaat tatgtggcag gccggtttcc tttttacaag cgcaggaagc
     4801 ctggatggga ttcatttcag gtgtgcccgg tgagagactc gcctatgtgg aaagcctgag
     4861 aaaagattac caggtttgcc tgttgagcaa taccaatcct tttatcatgg aatgggcgcg
     4921 aagtgcgcgt tttacccctc aaggccgggc actggatgtt tttttcgatc aactgttcat
     4981 ttcttaccag atgaaatgcg tgaaacctca caggcagatt ttccggcaag tactggatac
     5041 ccttggagca aaaccggaac acatactctt tgttgacgat ggtcctgcaa acattgaaac
     5101 agcaaaagat atgggattcc tgacattaat gcctgtaaac gggaccaact ggatcccgga
     5161 tgttcagaaa gtgcttgcaa atgccgttac aagacggaac aggggaaggt aacggttgat
     5221 gtacatgttg ccggaattat ccaggggggt atggtacacg tcatacgcat ccccgtcaac
     5281 catcatgtat agggcaggaa cctctttcat ggcaaaagga taatgatcgc tgtcattcga
     5341 gagtgggtgt tgctcaatgg taaacaaacc caggctgtcg ttaatgtgca ggaaacgctt
     5401 caggttttcc tctccctccg ccccggtttc cacaaacaac cgttccccgt tgtcggctac
     5461 catgtcagga tctatcacct gcagggtccg ttcaagggga aaaaccggat ttttggtgta
     5521 atgggttgat cccagcaggt tgttttcttc agctgctacg gcaataaaaa catacgtaag
     5581 ctccggtctg ttctcaggct gggcatagtg acgcgccagg gtcaggataa aagccactcc
     5641 cgaagcattg tcatgggcac ccggataata cacttcttta ccaaaatgtc ccaggtggtc
     5701 ataatgggca acaaacacaa tacaggagtc cgccctggaa gagccgggta tgtgcgctat
     5761 aacattggat gaagtataac cttcaatcat ccggctttca aaacggaccc tgatctgttt
     5821 tgcatcaccc gggaagcccg gtccggccca gatcacgggg atgggttgca cagtcccgct
     5881 ccgggacttg aaaaaagacg ggactttgtc ctggaccatg atcagacctg caacaggcat
     5941 tttgtatagt tcaaacgggg ctatgcccat gtatttgtat acaagatcgt attgcaatac
     6001 gacgtatgaa tttcggaaat caccgctttc cagcataggg atcagacgtg ccgggtcatg
     6061 atcctgcact ccgatatact tcaaggaaaa aatgcctttt tgtgaagggg agaattccct
     6121 gacaataaaa tctttgcccg gttccagcgt aaccccgtcc acggacattt ccattttctt
     6181 gtggaagaca ttaacgttgt atgaaaatgg ctgcaggtac cctccttcca cagaaggttc
     6241 caccccgcgg atctgcatga attgccgggc aatatatttt gcagctttcc tgtcaccttt
     6301 tttaaagtcg gaacgtccct cgtatttttt tgaggataat tccctgatga cttccttgta
     6361 gtaagttgtg tcctgtgcgt gtatacatgt gaaagataca agcaatgaac ctgtgattat
     6421 aaaataccgt agtctcatga cagcaaagat atattttttc tacatttgtg actatgaaac
     6481 gcttggcttt gatagtactg gcaatcgcag cgtttgcctg tacggaaaaa aacatccgga
     6541 ccatatctcc ggaagcagat gttgttatgg cccggttgca gcaggctgcc ggcaacaacc
     6601 aatttttctt tggacaggaa gactttccgt tttatggtca ttcctgggag tatgaagaag
     6661 gccgcagtga tgtgaaagag gtatgtggtg actatccggc cgtactggga tgcgatctgg
     6721 gccacataga actgggagat gataagaatc tggacggcgt gccttttgag attatgcgca
     6781 aagccataac ggaccattac atggcgggcg ggatagtcac aataagctgg cacgcccgca
     6841 atccgctaac ctcttccaat gcctgggatg tttcagacaa tactgtggtt gcctccattt
     6901 tggaaggcgg ctcacattac gatctgttcc agacctggct tgacaaggta gccgtttttc
     6961 ttgattccct gagaatggaa gacggaacac ggatcccggt gatcttcagg ccctggcatg
     7021 agcataccgg atcctggttc tggtggggca gaaaactttg ctccagccag gagtatatag
     7081 ccttatggaa gatgacagtg gattatatga gaaacaaggg gctggacaac ctgatcattg
     7141 cctattcccc taattacgga ggcctggacc gggaagcata catggagcgc tatccgggtg
     7201 atgattatgt ggatctgctg ggactggacg catacgggga cggacaggaa ttcctggacg
     7261 tcctgcgtaa tgaactgacc atggtcgccc agttgggcaa agagcataag aaagccatag
     7321 cccttacaga gtgtggcatg ggtggccttg gcaatcccct gtggtttaca caaatcctgc
     7381 taaaagccac ggaagggctt cctgtaacct atgccctcgt atggcgcaat gccccggact
     7441 acctgatgcc catgcatata ttcgctcctt atcccggaca cgcatcggcc aaagatttta
     7501 tcactttcta ccaggatccc cgcacactct tcctaaacga ttgacagacc atacatgaaa
     7561 aaactatatg taatcctgtt gatcctgttc cttacctctt cagttcctgc catatcgcag
     7621 aatgtacaat tgcattacag gacgggccag tggctatatc cggacacact tggaaaagat
     7681 gcccgcatcc tcactactgt ggaaatgttc cggatggacc cctggggaga tactttttta
     7741 tttgtcgata tgacgtacac cccgcaagga gtgaattacg cctattggga aattgcccgc
     7801 aacctgaaat tctgggatgc ccctgtggca gtacacctgg aatacaatgg cggattgctg
     7861 gggtccatat tgttcaacca ttcctggctg gcgggggtta attacggcat tgccagcaag
     7921 gacgggtcaa agtccttttc cgtttcggcc atgtacaaat atatccaggg attggccaga
     7981 ccttcgaatt ttcagttgac cgccatctgg aacatggagc ttgcccgcgg gaagtgcact
     8041 ttttccggtt ttgtggactg gtggagacag ggtgatactt atattctcct ttcccagccg
     8101 caattctggg tgaacctgaa cgcctttgag ggcatcagcg attctttctg tctgagtgtg
     8161 ggaaccgagg tggaattgta caacaatctt tttcacaaag gtttttatgt gatcccgagc
     8221 ctggcagtga agtggacttt ccgctaggaa aaccgttact gcatcacttc ccggatctcc
     8281 tgttccgtaa ccggcaaccg tttgctgccc agcagccggt gcccgtctgt cgtaatcagg
     8341 acatcgtctt ctatgcggat cccgccaaat ccatacagcg gtttgcattt ttcaaaattg
     8401 atgaatgcgg cattgtgccc gcgggctttc cacatttcca gcagcgcagg gatcacgtaa
     8461 aatcccggtt caacagtcat tacattcccc gcttccagca agcgtgccat acgtaacgat
     8521 ttaagcccga actgcgtgct cctggagaca gtgttgtcat agccgacata atgttctcca
     8581 aggtcttcca tatcgtgcac atccaggccc atgttgtgtc caagccccgt ggggaaaaag
     8641 gcggcatggg cccctgcggc cacagcttcc gcggtatctc ccctcatcag gccgaggtct
     8701 ttgagccctt ccgtgatgat ggttgcggct gccaggtgga catccctgaa gctaatcccg
     8761 ggggcgcaca tcccgataca gtggttgttg gtcttgagga ccaggttgta caggtccgct
     8821 gcaatcccct taaatacacc gcccaccggg atggtgcgtg taaaatcact gcagtaatgg
     8881 ctttgcagtt ccgccccggc atccatgagc agcaaccggc cttcggtcag gatctggtcg
     8941 tgggcgtgcc cgtgcataac ttctccgttc tgtgtcagga tgatcgggaa agaaggcata
     9001 tacccgccac tgacagcaat gccttccatg atacccgcca gttcctgttc gatcatcccg
     9061 ggttttgcca gtttcatggc cgtaaagtgc atggcgtaac caaggttgca ggcacggtct
     9121 atctgttcaa tctcttccgg ttgctttttc atacgcatgc caacaatggc gcggatgagc
     9181 tcttccgagg cttgctcttt cagttcgtta ggatggatat ccagcatgtc catcagttct
     9241 atcatgttgt cgctccggta gggtggcagg aaatgtacag tgcgtccttt ccttttctct
     9301 tcacggatat aggttgccag gtttttccgg ggtgaagtat gcgacaggcc tatcttttcg
     9361 gctttttcct gcatggcagg aagcggccct gtccagacaa tgtcatccag gctcacatcg
     9421 tctgcaaaaa gcacttcctt gcctgtttca aaatcaatga cggctgccag gtcaggctcg
     9481 tccagaccaa aataatacaa gaacgtactg tcctgcctga aatggtatgt gttgccgctg
     9541 taattgcagg gggcttcccg gtttcccatg aacaggccta ttcctttatt gagttttgac
     9601 ttgagtgtct gacggcgttg cgtgtatatt tccttagaga acataatggt tttatttttc
     9661 tcaaagatag caaaaagtca tgtacctttg ccgcatgctg cctacttctc gcaaagaact
     9721 ggaccgtctt ggatacggaa atgtcgatat catacttttt accggagacg cctatgtgga
     9781 ccacccgtca tttggggcgg cggtcattgg cagggtgctc caggcacaag gatacagggt
     9841 agccgttgtt ccgcagccca actggaggga cgatttgcgg gatttcagga aattgggtgt
     9901 gccgaggctt tttttcgcgg tgacttcggg caacatggat tccatggtaa accattatac
     9961 ggcggccagg cgcctgcgca gcaacgatgc
//
LOCUS       DAOSOF010000065         9921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6797_length_9921_cov_5.152747, whole genome shotgun sequence.
ACCESSION   DAOSOF010000065 DAOSOF010000000
VERSION     DAOSOF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_9921_cov_5.152747"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1360)
                     /locus_tag="PLM68_05000"
     CDS             complement(26..1360)
                     /locus_tag="PLM68_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT54756.1"
                     /translation="MDKPIKPFHHALGIRKEICIGCSRCLRVCPTEALRVKDGKASLH
                     PEWCIDCGECFNVCPVKAVVVEDDDIAQIFNYKHRILLVPSVFFAQFEEKLTLDAIKG
                     ILYDMGFTELCTVEQGADLLVDEINRYIQEYEGEKPVISSYCPAVVRLIQVRFPSFAD
                     RIMRLIPPLEITAQYYRNEYKKHDVPLEDVGIFYVTPCAAKIAAIKAPVGGYVSPING
                     VINMSELYNKVFLAYKQKGIYEHEHCALPHSMLSSVGVRWSTTCGEAANIRGRALAID
                     SMPNVIDFLERLENEEIKGVDFLELRACDMSCCGGILVQDNRFLIRERIEKTAEELPA
                     KHALDQEFKNVCSGYIPMETVEPRAMIKYHKDLTMALQRMEESRRLRRILPGIDCNAC
                     GAPSCEALADDIVCSRATLEHCVILHAHNKDPEMLEKIWGKNRFNKKNQDKI"
     gene            complement(1363..1776)
                     /locus_tag="PLM68_05005"
     CDS             complement(1363..1776)
                     /locus_tag="PLM68_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT54757.1"
                     /translation="MDLSFHIQGGDFTSAGTASSQVRRTLKELGVDAAVIKRVVVALY
                     EAEVNIVAHAYKGIITAEISPERVFLQLVDEGPGITDIEKAMTPGFSTASQEVREMGF
                     GAGMGLPNICRNADVFNIHSVPGKGTTVEITIQIK"
     gene            complement(1776..2120)
                     /locus_tag="PLM68_05010"
     CDS             complement(1776..2120)
                     /locus_tag="PLM68_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54758.1"
                     /translation="MTIHEIVSLVQGKILCGNVGEKDFVDYCFASDLMSDVLTVRRSD
                     FLLMTGLSNIQSIRTAEMSDLKYVMLVRGKTPPQPMIELAEENGIVLISCPFSMFKAS
                     GILYQAGLKGVF"
     gene            complement(2132..3253)
                     /locus_tag="PLM68_05015"
     CDS             complement(2132..3253)
                     /locus_tag="PLM68_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54759.1"
                     /translation="MTFEIKEISTRKEMKRFSRFPLQLYKNHPQYVPTLISDEIKTIL
                     DSPSLKYCSRKIWVASDSKNRIIGRVVGIVNSRANEIYGYKRVRFGWFDVIEDFDVAR
                     ALIQTVMDWGVSQGMTQIHGPLGFNTWNKQGLVVEGFENIPQFNCLYNYPYYGVYLER
                     MGFSKEVDWIQYMMSARQPVPDKIERINEAIMKRYNLHLLSWKKASDMKPYIDDFFDM
                     YNKSFMTVHNFIPLTPEEINDSIRFYMKLINPELSFFIVDGNNKVVAFSISFPSLSRA
                     LQKARGYLFPLGWFYLLKDYFFYKDIDLMMNGAHPDWKGKGLSSIYHYHTNKTAIRKN
                     LRRAVTNPQLETNHAIDVWKEYDTKLHTRRRCYIRNLNE"
     gene            complement(3268..3999)
                     /locus_tag="PLM68_05020"
     CDS             complement(3268..3999)
                     /locus_tag="PLM68_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT54760.1"
                     /translation="MEHWLSRSELLVGKNGLEKLNNSIVLVAGLGGVGSYAAEMLARS
                     GIGHLILADNDIITPTNRNRQLPALTGNEGALKTRVMEDRIRQINPDIVLETVSEYLE
                     EKSIPLLFDQFTPHYVVDAIDTLSPKIVLICYWMGRKTPLVSAMGAGARFDATAVKID
                     DISHSFNCPLAFMLRKRLRKLGIHKGLKVVFSTELPKEEAIVPLEEKNKKSRVGTISY
                     LPAVFGCLCAQAVICDILEIRTFGR"
     gene            complement(4003..4788)
                     /gene="mazG"
                     /locus_tag="PLM68_05025"
     CDS             complement(4003..4788)
                     /gene="mazG"
                     /locus_tag="PLM68_05025"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT54761.1"
                     /translation="MDRKQILESFGKLLDIMDRLRSECPWDREQTVESLRTLTIEETY
                     ELSEAILKGNLEDLSKELGDILLHIIFYAKIGQEKGAFDIGIVIENLCDKLIFRHPHV
                     FGDVKADNAGKVIKNWEQLKTREKNGNKSVLSGVPEGLPALIKSFRIQDKARAMGFDW
                     EKREQVWDKVNEEVQEFRTEWEKGERDRMESEFGDLLFALVNAARLYGINPENALERT
                     NRTFISRFNYLEEKTIKQGRSLEDMTLQQMDQIWEEAKHKAEK"
     gene            complement(4781..5542)
                     /locus_tag="PLM68_05030"
     CDS             complement(4781..5542)
                     /locus_tag="PLM68_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOT54762.1"
                     /translation="MPVVTVTSDWSKRDFYAGALVGGLLGLASVYEITHQIHPFDVRM
                     GVFVLRQAFTRFPAGSMHILAVQAQATDQLPMSVAFYQGHYFITVNDGRFSLLFDNEP
                     QWIRAVYAAPGTFSEQEAYIKAVRAIVEDKLQEMTLPADMKTEVRPSAVAEKDYIMGQ
                     VIYIDSYGNAITNISRSLFDRVGAGRDFRIFLQGPYTRIEKISDSYGGVRPGQLLALF
                     NSVNLLELAVFMGSLATLENIGIYDEIQIQFSKHG"
     gene            5645..6847
                     /locus_tag="PLM68_05035"
     CDS             5645..6847
                     /locus_tag="PLM68_05035"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOT54763.1"
                     /translation="MKRHSLQRNLIIAVLGFALLSSCNPSDQAGSLVVIPVRFNLPPV
                     SAERTFIVKTTENWYIEQVGHEDWCTVYPDKGTPGENQISLIVTPLPDEKPRLAIFVI
                     KSTSGIEKTLTVEQTNMDAQAYYLEFFGAWDPVIYVDPFDVEPIKQLVITNVPEWEVT
                     VSADAQDWITATKDQDTLIIGISDIDRPKGRTGSFTASAGGSISATLTISQQGLHDFL
                     APTRDFSLSIPGVPDSELHFSDVYSRARITLLLLWASWCPDCSGFMPEVMKLYNEFKD
                     HSFKIYGVAMEIEGREQDYFDYLENNGLNDVDETTGNKIWWENRPVFNPMAQVKVNAF
                     SRLLYGDALLTGEVMNYVPAFFFVDAGGNIKKVYTDPYVLRTDAAIRALYNNMRTYLS
                     RQLECCGN"
     gene            complement(6857..>9921)
                     /locus_tag="PLM68_05040"
     CDS             complement(6857..>9921)
                     /locus_tag="PLM68_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT54764.1"
                     /translation="SCGIGKCGACEMLVDGKVRRICITKVDGVKEVQRIPDGFLAEGG
                     TIPPSHTIKVYKTIVAIIGAGPAGLAVREELDKKGIPNLVIDNNVRIGGQFNMQTHQF
                     FFFEKEKRFGGMRGFEIAASLAGENHEGILLNSTVWDIFDNKRLAVKNIETQEIFYVD
                     AEYLVIATGSVPFMPPFGNDDVPGVYTAAVFQRMMNSELTLLGKNILTVGAGNIGYLT
                     SYQAMQAGARVRAIIEAMPHEGGFPVQANRVRRLGIPIITSHMLLEAIPNKDYTGISG
                     AVIASCRDFKVIPGTEKILKGIDTINICTGLLPDDQLLLKGRQAYGLNCHGVGDAIRI
                     GEGTSAVLRGKQCAYEIMQQMGVHIPYDDYLAVSKEYIDSQQHPVRILDKPALPSGER
                     MRAKPFVLFDCLYGFACNPCTFACKYGAIRKDTTSSVPVVDYDKCIGCMACVSQCPGL
                     AIFGFDLERNTLFLPVEYEVTAGAAVYLVDNQGRKKAEGTLEKIMTRPNKTHVARVRV
                     HEPVPADLLEISGFIVKEEYPEEPDLHPAGARKKIKTYICHCEDVTLEDLLKLIGDRD
                     SITADELKHISRMGMGACRGSRCLPRAVQILRSHGIQVISGLTPRGPMANLVQMGELY
                     PSGGQQEIILPKVINKEKDFEEAGVIIAGGGIAGSALFRYLAEEGFSPVLVNHDRGSS
                     WRNIAGGRPAFSLPALADISAQNLGIFKELDKKGNIDFHISRYVNFVHDQATFRSLEA
                     SMAWSDAHMVEARDFHKEISPAFNPYQKTYSHALITNNCWQASPGKTLDLIRHLGTEA
                     GGRILEQTRIVDVEKQGDTYTVILLDHRNRYQVFRTRLFINALGQNGEEFARKLGYIT
                     GVYPVRHQAFITKRLPMLGKDGKALDMLIDRRNYKGFSAVYGQQLADTGQIIGCASPV
                     SDMQESGKNLKVNTEDFLNIVSEVFIQWLPCLADAGFQAFWGGYYIEPRYIVDPGRGL
                     FLGMRGHGFMLSQYLAKLYVDVLSGRKVPDYFRDLSLDGPGLSEMAFK"
BASE COUNT         2434 a         2516 c         2250 g         2721 t
ORIGIN      
        1 aaggctgctg ccatttcttt gactgtcata tcttatcttg attttttttg ttaaaacgat
       61 tctttcccca tattttttcc agcatttccg gatccttatt atgggcatgc aggataacgc
      121 aatgctccag ggtcgcacgg ctgcagacaa tgtcatcggc cagtgcctcg caggagggag
      181 caccacaggc attgcaatcg atgcccggaa gtatgcgtcg tagcctgcgc gactcttcca
      241 tgcgctgcaa tgccatggtc aggtctttgt gatacttgat cattgccctg ggttcaacag
      301 tttccatggg tatatagccg gagcaaacat ttttgaattc ctggtccagc gcatgctttg
      361 ccgggagttc ctctgccgtt ttttcaatgc gttcccttat caggaagcga ttatcctgaa
      421 caaggatccc gccacagcag gacatgtcac atgccctcaa ttccaggaaa tccacgcctt
      481 tgatttcttc attctccagc ctttccagga aatcaatcac attaggcatg ctgtcaatgg
      541 caagggcacg tccccggata ttggcggcct ccccgcaagt tgttgaccag cgcacgccaa
      601 cagaggaaag catggagtga ggcagggcgc aatgctcatg ttcatagata cctttttgct
      661 tataggcaag aaatacttta ttataaagtt ccgacatgtt gatcaccccg tttatgggag
      721 atacataccc tcccaccggg gcttttattg ctgcaatctt tgccgcgcag ggagtaacgt
      781 aaaatatccc tacatcctcc agggggacat cgtgcttctt gtattcgttc ctgtaatact
      841 gggccgtgat ctccagagga gggatcagac gcatgatgcg gtctgcaaaa gagggaaaac
      901 ggacctgtat cagacgtaca acggccggac agtatgagga tatgacaggc ttttctcctt
      961 catactcctg tatataccgg ttgatctcat caaccagtag gtcggcaccc tgttccacag
     1021 tgcagagttc ggtaaatccc atgtcataaa ggattccctt gatggcatcc agggtaagct
     1081 tttcttcaaa ctgtgcaaaa aaaacagacg gaacaagaag tatccggtgt ttgtagttga
     1141 aaatctgggc tatatcgtcg tcttccacta caacggcctt gacgggacaa acattgaaac
     1201 actctccgca atctatgcac cattccggat ggagtgatgc cttgccgtct ttcacacgca
     1261 atgcctctgt cggacatacc cttaaacagc gagagcaccc tatgcagatc tctttgcgga
     1321 ttcccagtgc gtgatggaaa ggtttgattg gtttatccat actcatttta tttgaatagt
     1381 aatttcaact gttgtgcctt ttcccggaac agagtggata ttgaagacat cggcattacg
     1441 gcatatattg ggtaatccca tccctgcacc aaaccccatt tccctgacct cttgcgatgc
     1501 cgttgaaaat cccggggtca tggctttttc aatatcggtt atccccggcc cctcatcaac
     1561 aagctgaagg aaaacacgtt ctggactgat ctcggcagta atgatcccct tataagcatg
     1621 agcgacaata ttcacctcgg cttcataaag agctaccacc acccgcttga tcacggctgc
     1681 atccactccc agttctttaa gggttcgcct cacctgtgaa gaagcggttc ctgcagaggt
     1741 gaaatcacca ccctgtatat gaaacgacaa atccatcaaa aaaccccttt caatcctgcc
     1801 tggtagagaa tccctgatgc cttgaacatg gaaaaaggac acgatatcag cacgatccca
     1861 ttttcttcag ccagttctat catgggctgc gggggcgttt ttccccgtac cagcattacg
     1921 tacttaagat cactcatttc tgccgtacgg atactttgaa tattggacag accggtcatc
     1981 aacaaaaaat cgctcctgcg cacggtcagc acatcgctca tcaggtcgga ggcaaagcaa
     2041 taatcaacga agtctttttc ccctacatta ccacaaagta tctttccctg caccaacgat
     2101 actatttcat gaattgtcat tattatactt tttattcatt aaggtttctg atataacaac
     2161 gcctgcgcgt atgaagtttt gtgtcgtatt ccttccatac atcaatggcg tggttggtct
     2221 caagctgtgg attggtcacg gcccttctca aatttttacg gatggctgtt ttgtttgtat
     2281 ggtaatgata gattgagctc aacccctttc ctttccaatc gggatgggca ccattcatca
     2341 tcagatcaat atccttgtag aaaaaataat ccttcagcaa atagaaccag cccaatggaa
     2401 aaaggtaacc ccgtgctttc tgcagtgccc tggacaagct tggaaaggaa atggaaaagg
     2461 caaccacttt attattgccg tcaacaataa aaaaagagag ttccggattt atgagtttca
     2521 tgtaaaaacg gatactatca ttaatttcct ccggtgtgag ggggataaaa ttgtggaccg
     2581 tcatgaaact cttgttgtac atatcaaaaa aatcgtctat gtagggtttc atgtcgcttg
     2641 cttttttcca tgacagcagg tgaagattgt agcgcttcat gattgcctcg ttgatacgct
     2701 ctattttatc gggaacaggt tgccttgcag acatcatgta ctggatccag tccacttctt
     2761 ttgaaaaacc catacgttcc aggtaaacac cataataggg gtaattatag aggcaattga
     2821 actgtggaat attttcaaac ccttctacca ccagtccctg tttgttccat gtgttgaaac
     2881 cgagcggtcc gtggatctgg gtcatcccct gggagacacc ccaatccatt actgtttgga
     2941 taagtgccct ggcgacatca aaatcctcta tcacgtcaaa ccacccgaag cggacccgct
     3001 tgtagccgta aatctcattg gccctggaat ttacaatacc aacaactctt ccgattatcc
     3061 ggtttttgct atcgctggca acccagatct tgcggctgca atactttaag ctgggtgaat
     3121 ccagaatggt ttttatctca tcagagatca gggtgggtac gtattgggga tggtttttat
     3181 atagttgcag aggaaagcgg gaaaaccgct tcatctcttt tcttgtggag atttctttta
     3241 tttcaaaggt catttatctg ccattattta tcttccaaag gtacgaattt ccagaatatc
     3301 acaaataacc gcctgggcgc acaggcagcc aaaaacagcc ggaaggtagg atatggtgcc
     3361 tacccgtgat tttttatttt tttcttccag cggcactatg gcctcttcct taggcaattc
     3421 tgtggaaaaa acgactttaa ggcctttgtg gattcccagc ttgcgaagcc ttttacggag
     3481 catgaacgcc agcggacaat tgaagctgtg cgaaatgtcg tcgatcttga cagcagtggc
     3541 atcaaaacgg gctcctgctc ccatggcact gaccaggggt gtttttcttc ccatccagta
     3601 acagattagt acaatcttgg gagagagggt atcaattgcg tcgaccacat aatggggtgt
     3661 gaattgatca aaaagaaggg gtatcgattt ttcctccagg tactcgctga ctgtctcaag
     3721 aacaatgtca ggattgatct gccggatcct gtcttccatc acccgcgtct tgagtgctcc
     3781 ttcgttgccg gtcagggcag ggagctggcg gttgcgattg gttggtgtta ttatatcgtt
     3841 atctgccaga ataaggtgac ctattcccga ccgggcgagc atctcggctg cataggaccc
     3901 cacgccgccc agaccggcaa ccagaacgat actgttgttc aatttttcga gtccgttttt
     3961 tccaacaagc agttcggagc ggctcagcca gtgttccatg gtttattttt ctgctttatg
     4021 tttggcctct tcccaaatct ggtccatctg ctgcaatgtc atatcttcaa gggaacgacc
     4081 ctgcttaatg gttttttctt ccaggtaatt gaaacgggat ataaaagtcc tgttggttct
     4141 ttccagtgcg ttttcaggat ttatgccata cagcctggct gcattgacca gggcaaacag
     4201 cagatcaccg aactctgatt ccatcctgtc acgttcgccc ttctcccatt cagtgcggaa
     4261 ttcttggact tcttcattga ccttatccca cacctgttca cgtttctccc agtcaaaacc
     4321 catggcccgg gctttgtcct ggatacggaa tgatttgatc agggccggca aaccttcagg
     4381 aactcctgac agcactgatt tgtttccgtt tttttctctc gttttcaatt gttcccagtt
     4441 tttgatgacc tttccggcgt tgtctgcttt gacatcgcca aaaacatgcg gatgacggaa
     4501 aatgagcttg tcacatagat tttctataac aatccctata tcaaaggcac ccttttcctg
     4561 cccgatcttt gcataaaaaa tgatatgaag caggatatcg cccagttctt tggacaagtc
     4621 ctccagattt cctttcagga tagcctcgct tagttcatat gtttcttcta tggtaagtgt
     4681 ccgcaggctt tccacggtct gctccctgtc ccaggggcat tcggacctca ggcggtccat
     4741 aatatccagt agttttccaa aactttccaa aatctgtttt ctatccatgc ttgctgaatt
     4801 gtatctgaat ttcatcgtaa atacctatgt tttccaacgt tgccaggctt cccatgaaaa
     4861 cggccagttc cagcaggttg acactgttga acagggccag gagttgtccc gggcgaaccc
     4921 ctccgtaaga gtccgagatc ttttcaatac gtgtataggg tccctgcaaa aaaatccgaa
     4981 aatccctgcc tgcgcccacc ctgtcaaaaa gcgaacgcga tatgttggtt atggcattgc
     5041 cgtaggaatc tatatagatg acttgtccca ttatgtaatc cttttcggct acagcagaag
     5101 gcctgacttc ggttttcatg tcagcgggaa gggtcatttc ctgaagctta tcctcgacaa
     5161 tagccctgac ggcctttata taggcttcct gttccgaaaa agtccccgga gcagcgtaaa
     5221 cagcccggat ccattgcggc tcattatcaa acagcaagga aaaacggcca tcatttaccg
     5281 taatgaaata atgtccctgg tagaaagcta ccgacatagg taattggtca gttgcctgcg
     5341 cctgaaccgc aaggatatgc atgcttcccg ccggaaacct ggtaaaggcc tggcgcaaca
     5401 caaaaacccc catcctgacg tcaaaggggt gtatctgatg ggtaatttca taaacagaag
     5461 ccaatccaag caatcctcct accagcgcac cggcataaaa atcgcgcttg ctccaatcgc
     5521 ttgtaaccgt aaccacaggc atgactagat gttgattgaa tctgcaaatg taagcatttg
     5581 ggacgtgttt tggtttgtca tggaaaatgt tttactttac gcacacaaat tatgtagcaa
     5641 aacaatgaaa agacattctt tacaaagaaa cctgattatt gctgttttgg gatttgccct
     5701 gctttcttct tgtaatcctt cggaccaggc cggaagcctt gtggtgatac ctgtcaggtt
     5761 taaccttcct cctgtttctg ctgaaaggac ttttatagtc aagaccactg aaaactggta
     5821 tatagaacag gtgggacacg aagactggtg tacagtctat ccggataaag gcacccctgg
     5881 agaaaatcag atctccttga ttgttacacc tttacccgat gaaaaaccca ggcttgcgat
     5941 atttgtcatc aaatccacct caggtataga aaaaacactc actgtagaac agaccaatat
     6001 ggatgcccaa gcttattatc ttgaattttt tggagcttgg gaccccgtga tttatgttga
     6061 tccttttgac gtcgagccta taaaacaact ggttataaca aatgtacccg aatgggaagt
     6121 aacggtttct gccgatgcgc aagactggat aacggccaca aaggatcagg acacactgat
     6181 aataggcata tccgatattg accgtcctaa gggccgtaca ggttccttta ccgcatctgc
     6241 aggagggtcc atatctgcta ccctgaccat ctcccagcag gggttgcatg attttctggc
     6301 acccaccagg gatttttcat tgtcgattcc cggtgttcct gattcggaac tgcatttcag
     6361 tgatgtttac agcagggcac ggatcaccct gctcttgctg tgggcttcgt ggtgtcctga
     6421 ctgctccggt tttatgcccg aggtgatgaa gttatacaat gaattcaagg accatagctt
     6481 taagatctat ggtgttgcca tggaaatcga gggccgggag caggattatt ttgattactt
     6541 ggaaaacaac ggtctgaatg atgtggatga aaccacggga aacaagatat ggtgggagaa
     6601 cagacctgta ttcaatccca tggcacaggt aaaggtcaac gccttttcaa ggctcttgta
     6661 tggggacgcc ctgctcacag gggaagtcat gaattatgtt cctgccttct tctttgtaga
     6721 tgccggaggt aatatcaaaa aagtgtatac ggatccgtac gtgttgcgca ctgatgctgc
     6781 tatccgcgcg ttgtacaata atatgcgcac ttacctgagc cgtcagctgg aatgctgcgg
     6841 taattgagca gttaccctat ttgaaagcca tttcgctcag tcccggaccg tccagggaaa
     6901 gatccctgaa ataatccggg acttttcttc cactaaggac atctacatac aattttgcca
     6961 ggtactgcga aagcatgaat ccgtggccgc gcatgcccag gaaaaggccc ctgcccggat
     7021 caacaatgta cctcggctcg atgtaatacc cgccccagaa agcctggaat cctgcatcgg
     7081 cgagacaagg caaccactga atgaagacct cggatacaat attgagaaaa tcctccgtgt
     7141 tgactttcag gttctttccc gattcctgca tgtctgacac tggcgaggcg catcctatga
     7201 tctgtcctgt gtctgccagt tgttgcccgt aaacggcaga gaatcctttg tagttgcggc
     7261 ggtctatgag catatccaga gccttgccgt cctttcccag catagggagc cgcttggtga
     7321 tgaatgcctg atgtctgaca gggtaaacac ccgttatata gccaagcttc ctggcgaatt
     7381 cctccccgtt ttgtcccagg gcgttgatga aaagccttgt cctgaatacc tggtacctgt
     7441 tcctgtggtc cagcaggatc accgtatagg tgtcaccctg tttttccaca tccacaatcc
     7501 gtgtctgttc cagtatgcga cctcctgcct ctgtacccag gtgcctgatc aggtccaggg
     7561 tttttccggg actggcctgc cagcaattgt tggttatcag tgcatgggag tacgttttct
     7621 gatagggatt gaaagcggga gatatttctt tgtggaaatc ccgcgcctcc accatatgcg
     7681 catctgacca ggccatggaa gcttccagtg aacggaacgt tgcctggtca tgtacaaagt
     7741 tgacatacct ggatatatga aaatcaatat tgcccttctt gtcaagctct ttgaatattc
     7801 ccaggttctg tgctgaaata tcggccagtg ccggcaggct gaacgcagga cgtcccccgg
     7861 caatattcct ccatgagctc cccctgtcat ggttaacaag aacggggctg aagccttctt
     7921 ctgccaggta cctgaacagg gcgcttcctg caatgcctcc tcctgctatg ataaccccgg
     7981 cttcttcgaa atctttttcc ttgttgataa ccttaggcag gatgatctcc tgttgcccgc
     8041 cggacggata caattcaccc atctgcacca aatttgccat cggccccctt ggtgtcaaac
     8101 ccgagatgac ctggatacca tggcttctca ggatctgcac ggcacgggga aggcagcgtg
     8161 atccacggca agcacccatc cccatacggg atatgtgttt gagttcatcg gccgtgatcg
     8221 agtcgcggtc cccgataagc ttgagcagat cttcaagggt aacatcctca caatgacata
     8281 tgtatgtctt gatttttttt cttgcgccgg caggatgcaa atccggttct tccggatact
     8341 cctccttcac gatgaatccg ctgatttcca acaaatcggc cggtacaggc tcgtgtaccc
     8401 tgacacgggc cacatgtgtc ttgttggggc gggtcatgat tttttccagt gtgccttcgg
     8461 cctttttcct gccctggttg tccaccaggt acacagcggc tcctgcagtc acctcgtatt
     8521 caaccggaag aaacaatgta ttgcgttcaa ggtcaaaacc aaatatggcc aacccgggac
     8581 actggctaac gcaggccatg cagcctatgc atttatcata atccaccacc gggacggaag
     8641 atgtggtatc tttcctgatg gcgccgtatt tacaggcgaa agtacaggga ttgcaggcaa
     8701 agccgtaaag gcagtcaaac aacacaaaag gttttgccct catacgttcc cctgaaggca
     8761 acgcaggctt gtccaggatc cgtacagggt gttgttgaga gtcaatatat tctttggaaa
     8821 cagccaggta atcatcataa ggtatgtgca cacccatctg ctgcatgatc tcataggcac
     8881 actgcttgcc ccggagcacg gcagaggtgc cctctcctat tcgaatggca tccccaaccc
     8941 catgacagtt gagtccgtaa gcctgacggc ctttaagcag gagctggtca tcgggtaaaa
     9001 ggcctgtaca aatattgatt gtgtcaatgc ctttcagtat cttttcagtg cccggaatga
     9061 ctttaaaatc cctgcatgat gcaatcactg ctcccgatat tccggtatag tccttattgg
     9121 gtatggcttc cagaagcata tgggaggtaa tgatgggaat gcccagtctc cggacgcggt
     9181 tggcctgcac aggaaaccct ccttcgtgag gcatggcctc tatgatggcc ctgactcttg
     9241 ccccggcctg catggcctga taagaggtca ggtatcctat attgccggcc cctacagtca
     9301 gtatgttttt gcccagcagg gtgagctcac tgttcatcat cctctggaaa acggctgctg
     9361 tgtatacacc cggcacatca tcattgccaa aaggcggcat gaaggggaca gatcctgtcg
     9421 caatcaccag gtattcggcg tccacataga agatttcctg ggtttcaata tttttgaccg
     9481 ccagtctttt attgtcaaat atatcccaga ccgtgctgtt cagaaggatc ccttcatggt
     9541 tttcaccggc caggctggct gcaatctcaa atccgcgcat gcctccgaaa cgtttttcct
     9601 tttcaaaaaa gaaaaactga tgggtctgca tgttgaactg gccgccaatc cggacattgt
     9661 tgtcaattac aagattggga attcccttct tatcaagttc ttcccggaca gccaggcctg
     9721 caggccctgc tccaatgatg gccacaatgg ttttatatac cttgatggtg tgggaggggg
     9781 gaatagtgcc gccttctgcc aggaatccgt cgggaatacg ctgcacttct ttaactccgt
     9841 caacttttgt gatgcatatc ctgcgtacct tcccgtcaac cagcatctca caggctccgc
     9901 attttcctat cccgcaggag a
//
LOCUS       DAOSOF010000066         9748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_6961_length_9748_cov_5.041370, whole genome shotgun sequence.
ACCESSION   DAOSOF010000066 DAOSOF010000000
VERSION     DAOSOF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_9748_cov_5.041370"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="PLM68_05045"
     CDS             complement(<1..1944)
                     /locus_tag="PLM68_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT54765.1"
                     /translation="MKNYLWKRLFAGIFLLLLASGTSFAAYGDLQQSGRTVTCVVSDN
                     AGPVIGAYVVIRGTMNGGSTDADGRVVLNNVPDNTTLVVTFLGYVTQEIALMNNQTYV
                     EILLQEDAQALEEVVVVGYGVQKKKLVTGATVQVRGDDIAKLNTISPFTALQSQAPGV
                     TIVQNNGQPGSGFKVTIRGLGTIGASSPLYVIDGVVGGNLDNINPNDIESIDVLKDAA
                     SAAIYGARAANGVIMVTTKQGKLGKTTVTYDGFYGQQYLAKTPDLLTAQEYMVLQDEI
                     RFNEGNPSYDWANLLPADLYQSVMNGSWKGTNWFKEAYNEGAPTQNHAVGITGGSELS
                     RFSIGFSYSGTEGILGKPVQSQFERYTARINSEHVILKGRDFDIIKFGETLYYNYISN
                     NRVATGNIYSNAIHNLLVANPLLPIYNEEGDWFNYYDKQSTGWKFDGNTGNPIGAFSN
                     NGQGLNQSKSYSLRTTAYLEIQPIKNLVFRSQFGYNMSANTYRSYTAISAWSNNARTT
                     LDNVSQSGSMGHDWKIDNTLSYSFNLNDNQFDILIGQSAEKWGMGQSLNASGANSIFP
                     GYISPNTNINDVMDYAWVDNTKPTELSQRGAGGSVWGEGGIASFFGRINYNYKEKYMA
                     TLIFRADGSSNFARGKRWGYFPSA"
     gene            2387..3553
                     /locus_tag="PLM68_05050"
     CDS             2387..3553
                     /locus_tag="PLM68_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOT54766.1"
                     /translation="MVVFEDKLRILKKKHETLLLHANVPAEDPSGWYNRYQYPVLTPD
                     HIPLNWRYDLNPGTNPRLLLRYGINAVFNAGAILWEGRYLLVARVEGFDRKSFFAIAE
                     SPNGTDNFRFWERPVFMPEWGEPTTNVYDMRLVAHQDGWIYGIFCAERKDPAALPGDL
                     SSAIASAGIARTRDLVNWERLPDLESVSQQRNVVLHPEFIDGKYALYTRPQDGFIEAG
                     NGGGIGWALVDDITHARVSRETIANKRHYHTIKELKNGEGPAPLKTSKGWLHLAHGVR
                     NCAAGLRYVLYLYMTSLDEPWRVIAEPAGYFMAPQGEERIGDVSNVLFSNGWIRKEDG
                     TVLIYYASSDSRTHVASTSEDILLDYCFNTAPDGMTSAASVGVINDIINRNSQI"
     gene            complement(3566..4540)
                     /locus_tag="PLM68_05055"
     CDS             complement(3566..4540)
                     /locus_tag="PLM68_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOT54767.1"
                     /translation="MMKKPRYLIPHLFTADPSVHLFDGRLYIYPSHDIESGIPENDNG
                     DHFDMRDYHVFSMESPESEVTDHGVALDVKDIPWAGRQLWDCDVASRNGVYYLYFPLK
                     DKTDIFRIGVAVSRKPEGPFKPMDAPINGSYSIDPCVFRDDDGSHYMYFGGLWGGQLQ
                     RYRNNKAIECSHEPDDDQQALPSRVARLGDDMLRFAEDPKEVVLLDRNGDVLKAGDHD
                     RRFFEASWMHKYKGNYYFSYSTGNTHKLCYATGDNPYGPFIYQGVILTPVVGWTTHHC
                     IIEFKNRWYLFFHDSVPSGGKTWLRSLKVTGLDYNQDGSIITIDGGSV"
     gene            complement(4540..5673)
                     /locus_tag="PLM68_05060"
     CDS             complement(4540..5673)
                     /locus_tag="PLM68_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT54768.1"
                     /translation="MNISFKTMLLGLATLSAGAYTQAHQETQPSLKEAFSGKFLIGTA
                     MKAAQINETDTASVRVIKEHFNAIVAENCMKSMWLQPKEGVFDFTLADKFVEFGERNN
                     MIMYGHVLIWHSQAPAWFFTDSKGNDVTREVMIERMKTHIQTVMEHYKGRVKEWEVVN
                     EAIMDDGTFRKTKFYEIIGEDYIRLAFQFAREADPDSELYYNDYSMALPGRREGVVAM
                     VKKLQAAGLRIDGIGMQTHVGMDYPDLAEYEKSMEVFAALGVKIMITEMDITLLPFPD
                     QTAGADMNVSFEYQREMNPYAQGLPDSVNTLFEKRYLDFFSIFLRHKDMISRVTLWGV
                     SDQQSWRNNWPIPGRTDYPLLFDRQNKPKPVVSKIIEEALKTK"
     gene            complement(5696..>6249)
                     /locus_tag="PLM68_05065"
     CDS             complement(5696..>6249)
                     /locus_tag="PLM68_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54769.1"
                     /translation="FNAMGIVMTLVGIAISKPLADRYGKRDVFGTSLFLSALCLLMFY
                     FYSPRAIVPVFATQMLHGFFYGISTPLLWAMIADVADYSEWKNSRRATAIIFSAMIFG
                     LKAGLSIGGALVAGILASYGYNEQLAVQSAETVNGIKLSLSIYPTLTFFVSVACLFFY
                     EINKKMELRIQEDLAARRSDNEN"
     assembly_gap    6250..6349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6350..7197)
                     /locus_tag="PLM68_05070"
     CDS             complement(<6350..7197)
                     /locus_tag="PLM68_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54770.1"
                     /translation="MTFLAFFYTDVYKIPPGTASFIIFFGGMFGAFFNPIMGIIADRT
                     TTRWGKFRPWILWTAVPFGAMAILAFSTPDFSPSGKVVYAFVTYLLLVVVYSANNLPY
                     ASLSGVITGNMSERNSLSSYRFVAVTIAQLIVQSFLLPLALLLGDGDRTKGFENTMML
                     FAAIGVVFFIITFLTTKERIIPKPEQKSSIKEDLHDLIRNKPWIIMLIVTVFIFITLS
                     LKGGMYVYYFENYLSQEHLANFLEKVGISKLSGLGWPSDPATSGFSLFNAMGIVMTLV
                     GIAISKP"
     gene            complement(7304..>9748)
                     /locus_tag="PLM68_05075"
     CDS             complement(7304..>9748)
                     /locus_tag="PLM68_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT54771.1"
                     /translation="DRLGIPAYNWWNEACHGICYDNVTVFPQSIALAATFDAAQQYEI
                     YSAVSDEARAVWNTTDHNEFGKTKATGGIWHQGLSFWCPNINIFRDPRWGRGQETSGE
                     DPYLAGVMGTATVQGMQGDHKDYFKTHACVKHYAVHSGPEPLRHSFDATVSMRDLWET
                     YLPAFKEIVQVGNVQEVMCAYNRYEGEPCCGSDRLLQDILRDKWGYKSLVLSDCSAIN
                     NFYTKGQHETHPDAATASAAAVLAGTDLECGTSYEALIESLNKGLINETDLDIALRRI
                     LRSRIELGMFDPEDMIPWKDLDESVLSSPANTRLALEAARESMVLLKNDGVLPLSKDI
                     RTIAVVGPNANDVSMHNGNYNGIPTAENTISIVDAIRKAVPGTEIIYDKACELVDIYL
                     TTDHMGDINEGQGLYAEFFNNQEFEGTPANSGYYKTVNMRTFGDYRFADNVNQTDISA
                     RLTGKFVAGFTGNMYYSLLGSDTYKFTVNGRTVAQQKEPARGFGWFRSTVPSTTFPVV
                     KGQTYNIQVDFTKGGQGFAFLSFSLTERNLAEFSALAQRVKNADAIIVVGGISARLEG
                     EQMPVSLDGFNGGDRTRIELPDVQKELLAAMHKTGKPVILVNCSGSAIGFGDVETQYN
                     ALLQAWYGGQATGLAVADVLFGDYNPAGRLPVTFYKSTDQLPDFENYDMEGRTYRYFR
                     GEPLYAFGYGLSYTTFDYGNATLSRKSVKAGNGVKISIPVTNSGTYDGDEVVQVYVKS
                     LDNPEAPIKSLKGFKRVNIAAGTTATVSIELDSKAFEFYDESADELAPRAGKYQILYG
                     SSSKGSDLKALDFEVI"
BASE COUNT         2408 a         2302 c         2382 g         2556 t
ORIGIN      
        1 agcagagggg aagtaacccc agcgtttgcc gcgggcaaag ttagaagaac cgtcagcgcg
       61 gaagattaga gtggccatgt acttttcctt gtagttgtaa ttgatacgcc caaagaatga
      121 tgcaattccg ccttcgcccc agacagagcc gccggctccg cgttgagaca attcggtggg
      181 tttggtgttg tccacccagg cgtagtccat cacatcatta atattggtgt tgggagagat
      241 atagcccggg aatatggagt ttgcacccga tgcattcaga ctctgtccca taccccattt
      301 ttcagccgat tgacctataa gaatatcaaa ctgattgtca ttcaagttga aagaatagga
      361 caaggtgttg tcaattttcc aatcgtgtcc catactgcca ctctgactga cattgtcaag
      421 agtcgtcctg gcgttattcg accaggcaga aatggctgtg taggaacgat aagtgtttgc
      481 gctcatatta tagccgaact gcgaacggaa aaccaggttc ttgatgggct ggatttccag
      541 gtaagcggtt gtgcgaagcg aataactctt gctttgattg agtccctggc cgttgttgga
      601 gaatgcccct atggggttgc cggtattacc atcaaacttc caaccagtgg attgtttgtc
      661 gtaataattg aaccagtcgc cttcctcatt ataaatagga agaagagggt tggccaccaa
      721 caggttgtgg attgcgttcg agtaaatgtt accggtagct acacggttgt tgctgatgta
      781 gttgtagtat aaggtttcgc caaatttgat gatgtcaaaa tcgcggcctt tgaggatcac
      841 gtgttccgag ttgatcctgg cggtgtagcg ctcaaattgg gattgaacgg gtttcccgag
      901 gataccctct gtcccggaat aggagaatcc gattgagaat ctggacagct cgctgccgcc
      961 ggtaatccca acggcgtgat tctgggtggg agctccttcg ttgtaggctt ccttgaacca
     1021 gttggttccc ttccaggatc cgttcatcac ggactggtag aggtcggcag gaagaaggtt
     1081 tgcccagtcg taagagggat ttccttcgtt aaaacgaatc tcatcctgca agaccatata
     1141 ctcctgtgcc gtgagcagat caggagtctt ggccaggtat tgctgtccgt agaaaccgtc
     1201 gtaggtaacc gttgtttttc ccagttttcc ctgcttggtg gtaaccatga taacgccgtt
     1261 ggcggcacgg gcaccataaa tggcagccga agctgcgtct ttaagcacgt caatagattc
     1321 gatgtcattt ggattgatgt tatccaggtt acccccgacc acaccgtcaa taacatacaa
     1381 gggagaagat gctccaatcg tacccagccc gcggattgtg accttgaatc cagagcccgg
     1441 ctgaccgttg ttttgtacaa tagttacacc gggtgcctgg ctttgcagtg ctgtgaacgg
     1501 gctgatggtg ttcagcttgg caatgtcgtc accccttacc tggaccgtag ccccggttac
     1561 gagtttcttt ttctgaacgc cgtaacccac aaccactact tcttccagag cctgtgcgtc
     1621 ttcctgaagc aggatttcca cataggtctg attgttcatg agagcaatct cctgggtaac
     1681 gtagccaagg aatgtaacta ctagagtagt gttgtccggc acattgttca gaacaacacg
     1741 tccatctgcg tcagtactgc ctccgttcat ggttcccctg ataacgacat aggcaccaat
     1801 caccgggccc gcattgtcag aaaccacgca tgtaaccgta cgacctgact gctgcagatc
     1861 accgtatgca gcaaaggatg tacctgatgc taaaagcagc aggaaaatcc ccgcaaacag
     1921 acgtttccaa agatagtttt tcatacgtta agttttttgg ttaatagata gttataaata
     1981 tagtttatga atttggttta gtttttttga ataccgttta ttcaactttc aaatatacta
     2041 gctatacgga gaaaaaataa gtattatttt atctgaaaat gacacttttt tgtcataaat
     2101 caacaagtaa agtacactgg gtacgaaaaa ccaaggtaac aaaaccaact aacaaaagag
     2161 ttacaagata ataaaatttt cattcatttg tcccgaatgg atttcaaaaa aggtacaatt
     2221 tttgataaaa taatacttga ttacctcttt ttgtggaatt acttttgtca agtaaaagta
     2281 agacttgttg tatttaggtt taaaattagg ttaggtgggt caggagagtc gtcaggtgca
     2341 tggttaagaa atcgttgcat ggcgactctt tctggtaata gtaattatgg tcgtttttga
     2401 agataagttg cgcattctca aaaaaaagca tgaaacactg ctgttacacg caaatgttcc
     2461 ggctgaagat ccttcaggct ggtataaccg ctatcagtat cctgtcctca ctccggacca
     2521 tatacccctt aactggcgtt acgatctgaa tcccgggacg aatccacgtt tgttgctacg
     2581 atacggcatc aacgcggttt tcaatgcggg ggccatcctt tgggagggac gttacctgct
     2641 ggttgccagg gtggaaggct ttgacaggaa atcgttcttt gccatagccg agagccccaa
     2701 tggcaccgat aacttccggt tttgggaaag acctgttttc atgcccgaat ggggtgaacc
     2761 gaccaccaat gtttatgaca tgcgcctggt agcgcatcag gacgggtgga tttacgggat
     2821 tttctgcgcc gaaaggaaag atcccgcagc cctccccggt gacctgtcat cggccattgc
     2881 ctcggcgggg attgccagga ccagggatct ggtaaactgg gaaaggcttc cggacctgga
     2941 atcagtaagt cagcagcgga atgtggtgtt gcaccccgag ttcatagacg ggaaatatgc
     3001 cttgtacaca aggccccagg acggattcat tgaagccggg aacggcgggg gtataggatg
     3061 ggcgctggta gatgatatca cacatgcgcg tgtttcccgg gaaaccattg ccaacaaaag
     3121 gcattaccat accattaaag aattgaagaa cggggaaggc cctgcgcccc tgaaaacctc
     3181 aaaaggctgg ctgcacctgg cgcacggcgt caggaactgt gccgcaggtc tacgttatgt
     3241 gttatacctt tatatgacct ccctggatga gccctggcgt gtaatcgcgg agcctgccgg
     3301 ttacttcatg gccccgcaag gtgaagaacg catcggggat gtttccaatg tgctcttttc
     3361 caacggctgg atccgaaagg aagacgggac tgtattgatc tattatgcat ctagcgattc
     3421 acgcacgcat gtggcctcca caagtgagga tatactgctt gattactgtt tcaatacggc
     3481 gccggacggt atgaccagcg ccgcatcagt tggtgttatc aacgatatca taaacaggaa
     3541 cagtcaaatc tgacctgcgc ccctattaaa cgcttccccc gtcaatggtt ataatgctgc
     3601 cgtcctggtt gtaatccagc cctgtcactt tcagacttcg caaccatgtc tttcctcctg
     3661 aaggaacgct gtcgtgaaag aacaggtacc agcggttttt gaattctatg atgcaatggt
     3721 gtgtcgtcca gcctacaaca ggagtaagta tcactccctg gtagatgaat ggcccgtacg
     3781 ggttatcacc tgtggcataa caaagtttgt gcgtattgcc ggttgaatag gaaaaatagt
     3841 agtttccctt gtatttgtgc atccaggaag cttcgaaaaa acgcctgtcg tgatcacccg
     3901 ctttcagcac atccccgttc ctgtccaaca ggaccacttc tttggggtcc tcggcaaacc
     3961 taagcatgtc gtcacccagc cgggcgaccc ttgagggaag tgcctgctgg tcatcatcag
     4021 gctcgtgact acactcaata gccttgttat tgcggtaacg ctgcaactgt ccgccccaga
     4081 gtcctccgaa gtacatataa tgtgatccgt catcgtcccg gaagacacaa gggtcaatgg
     4141 aatagcttcc gttgatggga gcatccatgg gtttgaaagg tccttcaggc ttacggctta
     4201 ccgccactcc tatgcgaaaa atgtcggttt tatctttcag agggaaatac aggtaataga
     4261 caccgttcct ggaagcaacg tcacaatccc ataactggcg tccggcccag ggtatatctt
     4321 tcacgtccag ggccacaccg tggtcggtca cttcgctttc aggagattcc atggagaata
     4381 catgataatc gcgcatgtca aagtgatccc cgttatcgtt ttcgggaatg cccgattcga
     4441 tatcgtgcga gggataaata tagagccttc cgtcaaagag gtgaacggaa ggatctgccg
     4501 taaataaatg gggtataagg tatcttggtt ttttcatgat tattttgttt tcaatgcttc
     4561 ttcaatgatt tttgatacaa ccggtttggg tttgttctgg cggtcgaata acagaggata
     4621 gtcggttctt cccggaatgg gccagttatt cctccatgac tgctgatcgc tgacgcccca
     4681 gagtgttacc ctggaaatca tatctttatg ccttaagaaa atggaaaaga aatctaagta
     4741 ccttttttca aacaaagtat tcacagaatc gggcaatccc tgtgcgtacg gattcatttc
     4801 ccgctggtat tcgaacgaga cgttcatatc ggctcctgcg gtctgatcag ggaacggtaa
     4861 taaagtaata tccatttccg tgatcattat cttaacgccc agggccgcaa aaacttccat
     4921 gcttttttcg tattcggcca ggtccgggta atccatgccc acgtgggttt gcatgccgat
     4981 accgtctatc ctgagccccg ctgcctgaag ctttttcacc atagccacca cgccttcgcg
     5041 tctgccggga agggccatgg aataatcgtt gtagtagagt tcggaatcgg gatcggcctc
     5101 ccgggcaaac tgaaacgcca gtcgaatata atcctcgcca ataatttcgt agaatttggt
     5161 tttgcggaag gttccgtcat ccataatggc ctcattcacc acttcccatt cttttacccg
     5221 gcctttataa tgttccatta ccgtctgaat gtgtgttttc atacgctcta tcatgacttc
     5281 ccgggtcacg tcgtttccct tgctgtcggt gaagaaccac gcaggtgcct gggaatgcca
     5341 tataagaaca tgaccgtaca tgatcatatt attcctttcg ccgaattcga caaatttatc
     5401 ggccagagta aaatcaaata cgccttcctt tggctgaagc cacatgcttt tcatacaatt
     5461 ttcagccaca atggcattga aatgctcttt gatcaccctg acagaggcag tatctgtttc
     5521 attaatctga gcggctttca tggccgtacc tatcagaaat ttaccggaaa aagcctcttt
     5581 aagcgaaggc tgtgtttcct gatgtgcctg agtgtacgcg cccgccgaca acgtggccag
     5641 cccaaggagc atggttttga aagatatatt cataaatcgg tcctctggtt atttcttaat
     5701 tttcgttatc tgaacggcgt gcggccagat cttcctgaat ccgtagttcc atttttttat
     5761 tgatctcata aaagaacaga caggcaacgc ttacaaaaaa tgtgagggta gggtaaatgc
     5821 tcagtgacag cttgatcccg tttacagtct ctgcagactg aacggcaagt tgttcgttgt
     5881 aaccataact tgccaggata ccggcaacaa gggctccgcc aatgctcagg ccggctttca
     5941 gaccaaatat catggccgaa aatatgatgg ctgtggctct gcgtgaattc ttccattcgg
     6001 aataatcggc cacatcggca atcatggccc aaagtaaagg agtggaaatg ccgtagaaga
     6061 atccgtgtaa catttgcgtg gcaaaaacgg gcacgatggc acgggggctg taaaagtaga
     6121 acataagcag acacagggcg gagagaaaga gactggtccc gaacacatcg cgtttgccat
     6181 accggtccgc caggggcttg gaaatggcta ttccaacaag tgtcattaca atccccatgg
     6241 cattgaacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcttggaaat
     6361 ggctattcca acaagtgtca ttacaatccc catggcattg aacaggctga atcccgatgt
     6421 ggccggatcg gaaggccatc ccagaccgga cagtttgctt atgcccacct tttcaaggaa
     6481 attcgccaga tgctcctgac tcaggtagtt ttcaaaatag tacacgtaca tgccgccttt
     6541 aagcgacagg gtaatgaaaa tgaaaacagt cactatgagc atgattatcc agggtttgtt
     6601 ccttatcagg tcatgcaggt cttcttttat gcttgatttt tgttcaggtt tgggaatgat
     6661 cctttctttt gtggtcagga aagtgatgat aaagaatact acaccaatgg ctgcaaaaag
     6721 catcatcgta ttctcgaatc ctttggtcct gtctccgtcg ccaaggagaa gtgccagcgg
     6781 aagcaaaaag ctctgaacaa tcagctgggc aatggttacg gccacgaacc ggtaagagga
     6841 gaggctgttc cgttcggaca tgttacctgt tatcaccccg ctcagtgatg cgtagggaag
     6901 gttgtttgcc gagtaaacaa caaccagcaa caagtaagtg acgaaagcgt aaacaacttt
     6961 tccggaagga ctgaaatcgg gcgtactgaa ggccagtatg gccatggcac caaaaggcac
     7021 ggccgtccaa agtatccagg ggcggaattt accccagcgt gtagtggttc ggtctgcaat
     7081 aatgcccatg atgggattga agaaagctcc gaacatacca ccgaagaaaa tgatgaatga
     7141 agcggtgcct gggggaattt tgtatacatc ggtataaaaa aaggcgagaa acgtcataag
     7201 ggtttggaaa atcaggttag ccgacagatc acccaaactg tatcctattt tttctatgac
     7261 ggagatcttt tgtgtagaat tgctcataat caataattaa ggtttagatt acttcaaaat
     7321 ccagcgcctt gagatctgag cctttggaag aagaaccata gaggatctga tattttcctg
     7381 cacggggagc cagttcatca gccgattcat catagaattc aaaggctttg gagtccagct
     7441 ctatggaaac agtggcagtt gttcctgcag ctatattgac ccgtttgaat cccttgagag
     7501 acttgatggg tgcttcgggg ttgtccagac ttttgacata tacctggaca acttcgtctc
     7561 catcgtacgt gccgctgttg gtcaccggga tggatatctt aacgccattg ccggccttga
     7621 cgcttttcct ggagagggtg gcgttcccat agtcgaaagt ggtgtatgaa agaccgtagc
     7681 cgaaagcata caaaggttct ccgcgaaagt acctgtaggt gcgtccttcc atgtcataat
     7741 tttcaaaatc aggcagttgg tctgtggact tgtaaaaagt caccggtagt ctgccggcag
     7801 ggttgtagtc accaaagagc acgtcggcaa cagcaagtcc tgtagcctgt cctccgtacc
     7861 atgcctgcag caatgcattg tattgggttt caacatcccc gaaaccgata gcactccccg
     7921 agcaattcac caggatgacc ggcttgccgg ttttgtgcat ggctgcaaga agttccttct
     7981 gcacgtcagg aagctcgatc ctggtgcggt ccccgccgtt gaatccatcc agggaaacgg
     8041 gcatttgttc accttccagc ctggcagaaa ttcctcctac cacaatgatg gcgtccgcat
     8101 tcttcaccct ttgggcaaga gcgctaaact cggccagatt cctttctgta aggctgaagc
     8161 taaggaatgc aaagccctgt ccacccttgg tgaaatccac ctggatattg taagtctgcc
     8221 ccttgaccac agggaaggtg gtggaaggaa ctgttgatct gaaccaaccg aatcccctgg
     8281 caggttcttt ctgttgagca acagtcctgc cgttcactgt gaacttgtat gtgtcgcttc
     8341 caagcaggga atagtacata ttcccggtaa aaccggcaac gaatttacct gtcagtcggg
     8401 cagaaatgtc agtctggttg acattgtctg caaagcgata atcgccgaag gtacgcatgt
     8461 tcactgtctt gtaataacca gaattggccg gtgtcccttc gaattcctgg ttgttgaaaa
     8521 actcggcata caaaccctgg ccttcgttga tgtctcccat atggtctgtg gtcaggtata
     8581 tgtcaacgag ttcgcaggcc ttgtcgtaaa tgatctcggt cccaggtaca gcctttctga
     8641 ttgcgtccac tatggagatt gtgttctctg ctgttggaat gccgttataa ttgccgttat
     8701 gcatggatac gtcgtttgcg ttaggtccga caactgcgat tgtcctgata tctttggaaa
     8761 gtgggagtac tccgtcattt ttcagcagta ccatggattc cctggcagct tccagggcga
     8821 gccgggtatt ggcagggctg ctcaggacgc tttcatcaag atccttccac ggaatcatgt
     8881 cttcaggatc gaacatgcca agctcaatac gtgaacgcag gatgcgcctg agtgctatat
     8941 caaggtctgt ttcattgata agtcctttgt tgaggctttc gatcagcgct tcatagctgg
     9001 taccgcactc caggtcggtt ccggcaagaa cagccgcagc actggccgtt gcagcgtcgg
     9061 ggtgggtttc gtgctgtccc ttggtataga aattgttaat ggcactgcag tcagaaagga
     9121 ccagtgattt gtagccccac ttgtcgcgga gaatgtcctg gagaagtctg tcgctgccac
     9181 aacagggttc accttcatac ctgttgtaag cgcacattac ctcctgaacg ttccctacct
     9241 gtacaatttc tttgaatgca ggaagatagg tttcccaaag gtctctcatg gagactgttg
     9301 cgtcaaagct gtgcctgaga ggttccggtc cgctgtgcac agcatagtgc tttacgcagg
     9361 catgcgtctt gaaataatcc ttatgatcac cctgcattcc ctgtactgtg gctgtgccca
     9421 tcacgcccgc cagatacggg tcttctccac tcgtttcctg gccgcggccc catcttggat
     9481 cacggaagat gttgatattc ggacaccaaa acgagagacc ctgatgccat atgcctccgg
     9541 ttgccttagt ctttccgaac tcgttgtgat cggtagtgtt ccatacggca cgcgcctcat
     9601 ctgatacggc agagtatatt tcatactgtt gtgcggcatc gaatgttgca gccagtgcaa
     9661 tggactgggg aaatacggtg acattatcat agcagattcc atggcaggcc tcgttccacc
     9721 agttgtaggc ggggatcccg agccggtc
//
LOCUS       DAOSOF010000067         9701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7003_length_9701_cov_4.263218, whole genome shotgun sequence.
ACCESSION   DAOSOF010000067 DAOSOF010000000
VERSION     DAOSOF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_9701_cov_4.263218"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLM68_05080"
     CDS             <1..832
                     /locus_tag="PLM68_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT54772.1"
                     /translation="LEGRTGKQVAELIVTVCDLLLEEQGISAEEEKKVGICVPGIADT
                     RTDKVWAPNIPGWDSFPLKEYIIAAMPATETIIASDRTCYILGETWQGVAQGHTDAIF
                     LSVGTGIGAGIMVDGHVLHGAADIAGAIGWMALQPPFTDEYSNSGCFETFASGTGIAA
                     QARKLSGQPAVYLDARSVFEAYMRGNTVAMRVIDKAVECWGMASANLVSLFNPRMIVW
                     GGGVFGPAMKFLDRIYYEACKWAQPISIRQCRFEASSLSQKAGILGAGRLALEAMNVL
                     "
     gene            829..1968
                     /locus_tag="PLM68_05085"
     CDS             829..1968
                     /locus_tag="PLM68_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54773.1"
                     /translation="MNKRSILAAGYAGIFFFGISFLTLGSVLPSLREVLHLDMAQSSA
                     LAGILPFGVLAGSVVFGPWCDRFGYKMPFQFAAAMVVLGFIGLSAFTSVSWLGTCVFI
                     TGAGGGILNGACNGLVTDVSDDRNRSSNLSILGVFYGLGALVMPLILGIFDFVPYTAI
                     LRYMAVFLFLVFVYFSIIRFPEAKVRQGFPIKEGLSMLRQPALLFFSFILFFQSALEG
                     LCSNWIPIFLQDSAGYRLSQQNALFLLTFVVVGMTAGRILLSWLSFRYKPWKILWWYL
                     IVLAAGFTGLAAGVKPLVPVILIGTGLGATFPVIISYIGARYKSLSGTAIGIAMVIAL
                     AGNTLLNYLLGFLPIRVFPIYLLGIWTVMTLLYFLMTYRGKLDKS"
     gene            1980..2741
                     /locus_tag="PLM68_05090"
     CDS             1980..2741
                     /locus_tag="PLM68_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOT54774.1"
                     /translation="MLAIEWLDKVRHLQDRIQETQMDNIQKSATLMADSIEAGRWVHT
                     FGCGHATLPIEEMYPRIGGFVGFHPLIEQSLTFFTRITGESGIHQFLFLERAEGYGTA
                     IMSSWNFDSRDTLWIFSHTGINAVNIDVALEARKRGMKVIVYGSAAESTGKEPRHSCG
                     KNLFELADIVVDSCAPLTDASVTLKNHFDKIGPVSTMCFVTLVWMTVTTCAGILADRG
                     VKLHIHPSHNVPGDTTARERLDGCIEAYKTRTFGL"
     gene            2760..3401
                     /locus_tag="PLM68_05095"
     CDS             2760..3401
                     /locus_tag="PLM68_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54775.1"
                     /translation="MKKVILSLLLLILTISAYGQDNRSANISWYRNELFLDPARFFEG
                     TFALGYQRNFYNSALSLMPSVTLMGESIFRNEDAFYSEIEGFGMEGIYKVYFARMPRK
                     AQVYLGPYVAYRFLKEKNRLPDDVDPQDLPNITQSDLYTRYNTVMAGVLFGIHFMWGR
                     FTMDINVGGGIRYPTISGFRTNYTSIVTVPTGFAEFGYKGVVPKGNLTLGVAF"
     gene            3404..5389
                     /locus_tag="PLM68_05100"
     CDS             3404..5389
                     /locus_tag="PLM68_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT54776.1"
                     /translation="MRKVLLFWLGTLLSSVAIAQYIEQPASWSYSVLSVEENLFEIRF
                     QATLREPWHMYDLGPYQDGPQPTRFTFDLPEGAYLEDVMHMESPPAKEYDPLFNMEIG
                     SFGGKGVFIQKIHLEVPAALFRATVEWQVCDGTYCLPPEEEEFEIRLGEGPFLAGVSG
                     DAGELATDTAVDTAKEPSPSLWGIILQAVAFGLVALLTPCVFPMVPMTVSFFLRGSED
                     KRKSRLHAALYGLFIVLLYTVPIAVIIGITYFSGGPSVTANIFNWLATHWVPNILFFM
                     LFLVFAASFFGAFELVLPSKWTTKTDAKADKGGILGIFFLALTLVLVSFSCTGPIVGA
                     VLIKSTQGAVWEPIVTMFAFSTAFALPFTVLAFVPSLLQRLPKSGGWLNSVKVVLGFI
                     ILAFSLKFLSTADQVYQWGLLNRDIFLAIWIVIFSLLGFYLLGKIRFSHDSPSDHVGV
                     FRFSLAIAVFSFVVYLIPGMWGAPLRTLSGFLPPLRTQEFVLDRAAPSPVSAPGVTQP
                     ERKYGDLFELPYGLEGFFHYEQAMAYAKENNKPVFLDFTGLGCVNCKEMEARVWSDPG
                     VQELLRDKFVILALHSDAKNQADQDDWVIDERGKELRTLGRINSWFVRTRYQVNAQPT
                     YIILDRDGNPLLPPRNYDLDVEEYIRFLQKGLEVYHK"
     gene            5396..6760
                     /locus_tag="PLM68_05105"
     CDS             5396..6760
                     /locus_tag="PLM68_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOT54777.1"
                     /translation="MQGIQIFFNNLSSVLWGWPMIILLLGTHIFLTIRLKFPQRKLFK
                     GIALSVKSDGDSKGDVSQFGALATALAATIGTGNIIGVATAVSLGGPGAVLWCWLSGV
                     FGIATKYSEGLLAVKYRVTTSDGTMLGGPMYALERGLGMKWLAILFCVFTIIAALGIG
                     CSVQSNSIATMLKETFNVSQYVSGCIMAVLVALVIFFGIKGIARVCGFLVPFMAFFYV
                     VGCITILIMNAPFLGRALTLIFKSAFSVQAIGGGMAGGGLMIAMRYGIARGLFSNESG
                     MGSAPIVAAAARTRNPVRQALVSSTGTFWDTVVVCALTGLVLVTSIIRYPDINYTDGA
                     LLTKMAFSKISFFGPLILTIGLITFAFSTILGWCYYAEKAIEYLGGKKIIVYYRILWI
                     VGVFMGSVANLSMMWDLADTMNALMAIPNLISLLLLTRVLVKETNKYLWGNQLDAFDQ
                     DMVS"
     gene            complement(6757..7677)
                     /locus_tag="PLM68_05110"
     CDS             complement(6757..7677)
                     /locus_tag="PLM68_05110"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOT54778.1"
                     /translation="MKHIYTFLVVSVLAAGNASFAQTAQFLTLSTDTRTLSMGGTGVV
                     APANAFSLYNNNAAAALSPGKGALAASYTLWQPGATGSNLANLSGFFKIGKKSAFLIG
                     SRFHLNSPYNITNEHGSITGTYRPMELSIDLGYAFKITETFSTAATLRYVISRMGEDA
                     SGGAFAADLGVIYKPGNFSLGLTATNIGSKMDYGYGPYELPAQIRLGAGYGFHAGEKH
                     FLCAAAQTGYLIKNSGFTAGIGLEYVYNGMVSARLGGHYGDPEKGIPPHLSVGLGFTY
                     RGLSLNAAYLAGQKGSAVSNSLSFGLEWAF"
     gene            complement(7685..>9701)
                     /locus_tag="PLM68_05115"
     CDS             complement(7685..>9701)
                     /locus_tag="PLM68_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT54779.1"
                     /translation="VEITHPDLVNNLWVNLAELNGVEGVDDDNNGIVDDINGANFITH
                     NGYIRAEDHGTHVAGIVAATNNNATGACGIAGGNGVQTGVRMMICQIMDEYNSVGDAA
                     GGIKYAADNGAVICQNSWSYVESNNLPQSTKEAIDYFITYAGYDENGNQTGPMAGGLV
                     VFAAGNDNTSISYPAMYEKVFSVAAIAPDYKKAYYSNYGDWVSISAPGGDANYSSGQI
                     YSTLKNGQYGFRQGTSMACPQVSGVAALVVSYAGGPGFTNEELKRILKQNASPVIFSY
                     YPQYAGIMGSGVVDAFASIASLSTIPPDAVADIDAVAYSNNMTLTWLVPRDEDDVKAY
                     RYEVLISSIAGDSLQVSHVRTGNNEAGEIVSHQISGLDFNTSYIFTITACDFAGNRAD
                     ASEPITAQTEANNPPLIEPLDGTQLVLKAHQEKTLSFRISDPDGHTLTAAVEPASPEL
                     FIGFKDTLVTVRFDGKLAPAGDYVVSLKVNDPYGGEAKIQINYTILPNTTPRVLATPE
                     NIIFNRTGAGKTLKLTDYITDDDGEILEFTVTFNPQGFATGTISDGLLNVTGRKYGLA
                     TITVTATDALKASCSVDILVLTRDGNQPVDLYPNPVTDILNIRTGETTPAEITITSSN
                     GARVFRGTFDLSPFQPAKIDMTAQSGGVYAVSINIKGEIITRNIVKL"
BASE COUNT         2218 a         2258 c         2501 g         2724 t
ORIGIN      
        1 tctggaaggc cgtacaggca agcaggtggc agaactgata gtaacagtct gtgacctgtt
       61 gctggaagaa cagggtattt ccgctgaaga agaaaagaag gtagggattt gcgtgccggg
      121 aattgcagat acacgtacag acaaggtctg ggcgcccaac attccaggct gggacagttt
      181 tcctctcaag gaatacatta tcgctgcaat gcccgctaca gaaactataa tcgcaagtga
      241 cagaacatgc tacatactgg gagaaacatg gcagggcgtt gcacaagggc ataccgatgc
      301 catttttctg agtgtgggca caggaatagg agcagggatc atggtggacg gacacgtgtt
      361 gcacggtgcg gctgacatag cgggagccat tggatggatg gcactgcagc ctccatttac
      421 cgatgaatac agcaacagtg ggtgttttga aacgtttgca tccggaacgg gaattgccgc
      481 acaggcccga aaattatccg gccagcccgc tgtatacctg gacgcccgct cggtttttga
      541 agcgtatatg cgtggtaata cggtggcgat gagagttata gataaagcag tcgaatgttg
      601 gggtatggct tcggcaaacc tggtgagcct gtttaatccc cgcatgattg tctggggtgg
      661 aggtgttttt ggacctgcca tgaaatttct ggacaggatc tattatgaag cctgcaaatg
      721 ggcacaacca atcagtataa gacaatgccg gtttgaagct tcgagcctgt cccagaaagc
      781 ggggatcctg ggtgcggggc gcctggcact ggaagcaatg aatgtattat gaataaacgt
      841 agtattctgg ctgccggcta tgccggcatc ttttttttcg gaatatcttt tctcaccctg
      901 ggatcggtat tgccatccct tagggaggta ctgcatcttg acatggcaca atcttctgcc
      961 ctggccggga tcctgccttt tggcgtcctg gccggttcgg ttgtgttcgg accctggtgt
     1021 gaccgcttcg ggtataaaat gcctttccag tttgctgcag ccatggtggt gctgggattc
     1081 ataggattgt ccgcctttac ttcagtgtca tggctgggta cctgtgtgtt catcacgggt
     1141 gcgggaggag gcattcttaa cggggcctgc aacggcctgg tgaccgatgt gtccgatgac
     1201 cgcaacagga gctctaacct gagcattctg ggtgtttttt atggcctggg cgccctggta
     1261 atgcctttga tactggggat atttgatttt gttccctaca cagccattct tcgttacatg
     1321 gctgttttcc tgttcctggt atttgtatat ttcagtataa tacgctttcc cgaggccaag
     1381 gtccggcagg gatttcccat taaggaggga ttgtccatgc ttcgacagcc ggctttgttg
     1441 ttttttagct ttatcctttt tttccagtct gccctggaag gattgtgcag caactggatc
     1501 cccatttttt tacaggatag tgcaggttac aggctgtcac aacagaatgc gttatttctg
     1561 ctaacctttg tagtagtagg tatgactgca ggcaggattc ttttgagctg gctttcattc
     1621 cgctataaac cctggaagat cctttggtgg tatctgatag tgttggctgc aggatttacg
     1681 ggattggcag ccggggtcaa gccgttggta cctgtgattc tgatagggac aggacttggg
     1741 gcaactttcc cggtcattat ttcctatatc ggggcccgct acaaatcatt gtccgggaca
     1801 gccataggga tagccatggt catagccctg gcgggtaata cattgttgaa ttatttactg
     1861 ggctttctgc ccattcgcgt ttttcccata tatttgcttg ggatatggac cgtaatgact
     1921 ctcctttatt ttttgatgac gtatcgtgga aagcttgata aatcttaata ttattgaata
     1981 tgcttgcaat agaatggctg gataaggtcc ggcaccttca ggacaggatc caggaaacac
     2041 agatggataa tatacaaaaa tcagcaaccc tgatggctga cagcatagaa gccggccgtt
     2101 gggttcatac tttcggatgt ggtcacgcca cactccctat tgaggagatg taccccagga
     2161 tagggggatt tgtgggattc cacccgctga tcgagcaatc ccttaccttt tttacccgta
     2221 taacgggaga gagcggtatt caccagtttt tattcctgga aagggcagaa ggatacggga
     2281 cagccatcat gagttcctgg aattttgact cccgggatac actctggata ttttcccata
     2341 ccggcatcaa tgctgtgaat attgatgtag cgctggaagc cagaaaacgg ggcatgaaag
     2401 tgatcgtgta tgggtcggca gctgaatcta cggggaaaga accacgtcat tcctgtggaa
     2461 agaacttatt tgaactggcc gatattgtgg tggattcctg cgcgccactt acagatgctt
     2521 ccgttacgct caagaaccat tttgacaaga taggccctgt ttccaccatg tgctttgtaa
     2581 cattggtatg gatgactgtc accacctgcg ccggcatcct tgcggacagg ggtgtaaaac
     2641 tgcacatcca tccttcacac aacgtccctg gtgacactac agcaagggaa cgccttgacg
     2701 gttgcataga ggcctataaa acaagaactt tcggattata aactaaatat agatagtcta
     2761 tgaaaaaagt gattttaagt ctgttgttat tgatcttgac tattagtgcc tatggacagg
     2821 acaatagatc cgcaaatatt agctggtaca ggaatgaatt gttcctggat cctgcccgat
     2881 tctttgaagg aacctttgcc cttggttacc agaggaattt ttacaattct gccctgtcgc
     2941 tgatgccatc tgtcaccttg atgggagagt ccattttccg taatgaagat gcattttaca
     3001 gcgaaataga aggattcggg atggagggaa tatacaaagt ctacttcgca cgcatgccgc
     3061 gcaaagcaca agtttacctg ggaccttatg tagcctatcg tttcctgaaa gaaaaaaaca
     3121 ggttgcccga tgacgtagat ccccaggacc tgcccaacat aacccaaagc gatttatata
     3181 cacgttataa tactgtaatg gcaggggttt tatttggtat ccatttcatg tggggacgtt
     3241 ttaccatgga tatcaatgtc gggggaggaa tccgatatcc taccatttca ggtttcagga
     3301 caaactatac ttccattgtt acagtgccaa caggttttgc ggagttcgga tataagggtg
     3361 tggtccccaa aggtaattta accctgggcg tagcgttcta gtcatgagaa aagttctttt
     3421 gttttggctg gggaccttat tgtcttcggt tgctattgca cagtatatag aacaaccggc
     3481 ctcgtggagc tacagcgtat tgtctgtcga agaaaactta tttgaaattc gttttcaggc
     3541 cacattaagg gagccatggc acatgtacga tctggggcct tatcaggatg gtccgcaacc
     3601 cacccggttt acttttgatt tgccggaagg tgcctacctg gaagatgtta tgcacatgga
     3661 atcgcctccg gccaaagaat acgacccttt gttcaatatg gaaatcggtt cgttcggagg
     3721 gaaaggggta tttattcaga aaatacacct ggaagttccg gcagccctgt ttcgcgccac
     3781 tgttgaatgg caggtatgtg acgggacgta ctgtctgcct ccggaagaag aggaattcga
     3841 gatccggctg ggagagggac cctttcttgc aggtgtttcc ggtgatgcag gtgagttagc
     3901 aacggatact gctgttgata cggcaaagga accatctccc tcgctttggg gtatcattct
     3961 gcaggcggtc gcattcggcc tggtggccct gttgacgccg tgtgtttttc ccatggttcc
     4021 catgaccgtt tctttctttc tcagggggag tgaagacaag cgcaaaagcc ggctgcatgc
     4081 ggctttatat gggcttttca ttgtactttt atatacggtt cccattgccg tgatcattgg
     4141 tatcacttat ttttcaggag gaccttcggt aacggccaat atctttaact ggcttgcgac
     4201 gcactgggtg ccgaacatcc ttttcttcat gctgttcctg gtatttgcag cttctttctt
     4261 cggggctttc gagctggtac ttccctcaaa atggactaca aaaacagatg ccaaagcaga
     4321 caaagggggt attctgggga tatttttcct ggcactcacg ctcgtgctgg tttctttctc
     4381 gtgtacaggt cccatagtcg gggcggttct gatcaaatca acccagggag cggtctggga
     4441 acctatagtg acaatgttcg ccttttctac tgcttttgca ctgccattca ccgtcttggc
     4501 ctttgtgcca tccctgttgc aacggcttcc aaagtcaggc ggctggctca attcggtaaa
     4561 ggttgtactg ggcttcatca tactggcgtt ttccctcaaa ttcctgagca ctgcagacca
     4621 ggtataccag tggggattac taaaccgtga cattttcctg gccatatgga ttgtcatttt
     4681 ttctttgctg ggtttttacc tgttgggcaa gatacgtttc agtcatgatt caccttctga
     4741 ccatgtgggt gttttccgtt tttctcttgc tatagctgtt ttctcttttg tggtgtacct
     4801 gatcccgggt atgtggggag cgcctctcag gaccctctca ggtttccttc caccgctgcg
     4861 cacacaagag ttcgtacttg accgtgcggc tccgtctccc gtttctgcac ctggagtgac
     4921 acagccagag aggaagtacg gtgacctttt cgagctgcct tacggacttg aaggattctt
     4981 tcattacgaa caggccatgg cttatgcaaa agaaaacaac aagcctgttt tcctggattt
     5041 cacaggactg ggctgtgtca attgcaagga aatggaagct cgcgtgtgga gcgatcccgg
     5101 ggtacaggaa ttgctcaggg ataagtttgt aatactggcc cttcattcag atgccaagaa
     5161 tcaggcagac caggacgatt gggttattga cgaacgggga aaggaattac gtacccttgg
     5221 aaggataaac tcctggtttg tacgcacccg gtatcaggta aacgcacaac ctacctatat
     5281 catcctggac agggacggga atccgctgct ccctccccgg aactatgatc ttgacgttga
     5341 agagtatatc aggtttctgc aaaaaggact ggaagtttac cataaataat gctatatgca
     5401 aggcatacaa atttttttca acaatctcag cagtgtgttg tggggctggc ccatgattat
     5461 tcttttactt ggcacccata tttttctgac catccggtta aaatttcctc aacgtaaact
     5521 atttaaaggc attgccctat cggtcaaaag cgatggtgat tccaagggtg atgtcagtca
     5581 gtttggcgcc ctggccacag cattggccgc taccatagga acagggaata tcatcggggt
     5641 agccactgcc gtttcattgg gcggtcccgg ggctgtgcta tggtgctggc ttagcggggt
     5701 tttcggcata gccactaaat attcggaagg gctactggct gtaaagtacc gggttacgac
     5761 ctcagacggc accatgctgg gggggcccat gtatgcgctg gaacgtggtc tgggcatgaa
     5821 gtggctggcc atcctgtttt gtgtttttac cattattgct gccttgggaa tcgggtgctc
     5881 tgtccagtcg aattccatag ctaccatgct taaggagacc tttaatgtct cacaatatgt
     5941 ttccgggtgt attatggcag ttttggttgc tctggttatt tttttcggca tcaagggaat
     6001 tgccagggta tgcggttttc tggtcccttt tatggctttt ttttatgttg tgggttgtat
     6061 cactatcctt atcatgaacg cgcctttcct gggacgggcc ctgaccctga ttttcaaatc
     6121 ggctttttct gttcaggcta tcgggggtgg catggcaggc gggggcctta tgattgccat
     6181 gcgctatggg atcgcaaggg gattgttttc caatgaatcg ggcatgggct ccgcaccaat
     6241 tgttgccgca gctgcaagaa caaggaatcc ggtgaggcag gctctggtat catccacggg
     6301 aactttctgg gatacagttg ttgtctgtgc cctgacaggt cttgttctgg tcaccagcat
     6361 tatcaggtat ccggatatta attatacaga cggtgccttg cttacaaaaa tggctttcag
     6421 caagatctct tttttcggac ctcttatcct tacaatcgga cttatcacgt tcgctttctc
     6481 caccatcctg ggttggtgtt attatgcaga aaaggccatt gaatacctgg gaggcaagaa
     6541 gataatcgta tattaccgga tactttggat cgtgggagtc tttatgggat ctgttgccaa
     6601 cctgtccatg atgtgggacc tggccgatac catgaatgcg ctcatggcca ttcccaacct
     6661 gatatcgtta ttgctgctta caagagtcct tgttaaggaa accaataaat atctttgggg
     6721 caaccaactg gatgcctttg accaggacat ggtgtcctaa aaggcccact ccagtccaaa
     6781 gctcaagctg ttagataccg cagatccttt ttgtcccgcc aggtaagccg cgttcaggga
     6841 cagcccgcgg taagtgaatc ccagtccgac gctcagatga ggcgggattc ctttttccgg
     6901 atcgccataa tggcctccca ggcgggccga gaccatcccg ttgtaaacat attccaatcc
     6961 tataccagcc gtaaatcccg agtttttgat caaatatccc gtctgtgcag ccgcgcagag
     7021 aaaatgcttt tccccggcat ggaaaccgta gcctgccccc agcctgatct gtgccggaag
     7081 ttcatacggc ccgtaaccat aatccatttt ggagccgatg tttgtggcgg taagtcccag
     7141 ggaaaagttc ccgggtttat atatcacacc caggtctgca gcgaaggctc cgccagaagc
     7201 atcctcaccc attctggaaa taacataacg aagggtggcc gctgtggaaa aagtttctgt
     7261 aatcttaaag gcataaccca ggtcaatgga aagttccata ggtctataag tccccgttat
     7321 gcttccatgc tcattggtaa tgttgtaggg agaattcaga tgaaagcgtg accctattaa
     7381 aaaggcgctt tttttaccga ttttaaaaaa accggacaag ttagccaggt tgctgcctgt
     7441 ggcaccgggt tgccaaaggg tataggaagc cgctaacgct cccttaccgg gagaaagtgc
     7501 agcggcagca ttgttgttat acagagaaaa agcatttgcc ggagccacca caccggtccc
     7561 tcccatggac agtgttctgg tgtccgtgga cagtgtcagg aattgcgctg tttgtgcgaa
     7621 tgaggcgttg ccggcagcca ggacagagac aacaaggaaa gtatatatgt gtttcatgat
     7681 cggtttacag tttcactata tttctggtga ttatttctcc tttgatgttt atgctgactg
     7741 catatacgcc cccgctttgg gccgtcatat ctatctttgc cggctggaat ggggataaat
     7801 caaaagtgcc cctgaacacc cgggctccgt tggaagatgt tatagtgatt tctgccggcg
     7861 ttgtttctcc cgttctgata ttcaggatat ctgtcacagg attcggatag aggtccaccg
     7921 gctggttccc gtcacgggtc agcacaagga tgtcaacact gcaacttgct tttaaagcgt
     7981 cagtggcagt cacggtaatg gtggcaagtc cgtattttct acctgtcaca ttgagcaatc
     8041 cgtcgctgat tgttccggtg gcaaaaccct ggggattgaa agtaaccgtg aattccagga
     8101 tctctccgtc gtcgtctgta atataatcgg tcaattttaa agttttgcct gcccctgtcc
     8161 tgttaaaaat gatattttcc ggagtcgcca gtaccctggg ggttgtgtta ggcaggatgg
     8221 tataattgat ctgtatcttt gcctccccgc cataaggatc atttaccttc agggatacaa
     8281 cgtagtcacc agctggtgca agctttccat caaacctcac agtcaccagg gtgtccttga
     8341 atcctatgaa cagttcagga gaggccggtt ccacggctgc ggtaagggtg tgaccgtcag
     8401 ggtcagagat cctgaatgag agtgtttttt cctgatgggc cttgagaacc agttgagtgc
     8461 cgtcaagtgg ttcaataaga ggagggttat tcgcttcggt ttgtgcggtt attggttccg
     8521 aggcatcggc ccggtttccg gcaaaatcac atgcagtgat tgtgaatata taggaggtat
     8581 tgaaatccag gcctgagatc tggtgcgata ctatttctcc cgcctcgttg tttcctgttc
     8641 gcacatggga cacctgcagg ctgtccccgg caatggaaga aatcaatact tcatacctgt
     8701 aagctttgac atcgtcctcg tcccgtggta ccaaccaggt taaagtcatg ttgttggaat
     8761 aggctactgc gtcaatatca gccactgcat ccggaggaat ggtgcttagc gaggcaatgg
     8821 aagcgaatgc gtcaaccaca ccacttccca tgatgcctgc atattgcggg taatagctga
     8881 agatgacagg agatgcgttt tgtttaagga tgcgttttaa ttcctcgttt gtaaagcctg
     8941 gtccccccgc gtaagaaaca accagggcag ccacaccgga tacctggggg catgccatgg
     9001 atgtaccctg ccggaagccg tattggccat ttttcaaggt actgtagatt tgacccgatg
     9061 aatagtttgc atcaccgccc ggagcgctta tgcttaccca gtcaccgtaa ttggaataat
     9121 aagccttttt ataatccggg gcaatagcgg ccacagaaaa gactttttca tacatggcgg
     9181 ggtaggatat actggtgttg tcgtttccgg cggcaaaaac taccagaccg cccgccatgg
     9241 ggcctgtctg gttgccgttt tcatcatagc cggcatatgt tatgaaataa tctatggctt
     9301 ctttggtgct ttggggcaaa ttgttgctct caacatagct ccagctgttc tggcatataa
     9361 cggctccgtt gtctgcagcg tatttgatcc ctccagccgc atctcccacg ctgttatact
     9421 catccatgat ctggcatatc atcatacgca ctcccgtctg aacaccattt cccccggcaa
     9481 tcccgcatgc acctgtggcg ttgttattgg ttgccgctac aattccggct acatgggtcc
     9541 cgtggtcctc tgccctgatg tatccattgt gggtgatgaa atttgcccca ttgatgtcat
     9601 ccactatgcc gttgttgtca tcgtccacgc cctccacgcc gttcagttcc gccaggttga
     9661 cccataaatt gttcaccagg tcgggatggg ttatttccac c
//
LOCUS       DAOSOF010000068         9626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7063_length_9626_cov_4.667746, whole genome shotgun sequence.
ACCESSION   DAOSOF010000068 DAOSOF010000000
VERSION     DAOSOF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_9626_cov_4.667746"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLM68_05120"
     CDS             complement(<1..397)
                     /locus_tag="PLM68_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOT54780.1"
                     /translation="MNISERIKDLRHQMLQLGWDAAIISGTDPHGSEYLPERWQQRKW
                     ISGFTGSYGTVVITHAHAGLWTDTRYFIQAREELEGTEFEIHPLRVPHAVDYPQWLAE
                     NLPAGSVTGIDGDCMTYSQVEKLFASLQAN"
     gene            complement(394..1692)
                     /locus_tag="PLM68_05125"
     CDS             complement(394..1692)
                     /locus_tag="PLM68_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54781.1"
                     /translation="MMKPESGIRSFPRNFWTAISMEFFERGAYYGVLSILSVYLVLSP
                     DEGGLGFSREAAGAIMGTIPPLLYFLPLFAGALADRYGFRNILMVAFIFLTTGYFLTG
                     FMTSYGMVFASLVVMAIGAGFFKPVISGTIAYSTTEKNSTLGFGIFYWSINLGAFLFP
                     LLLVPVLKKESYSYVFWMAAAAGVILLLMNIFLYREPVRAREKVPFSKIFSDMALVFK
                     DWKFIVMILLYSGFWIMYFQMYGTVLWYVNEYMDMTPVNDTVNAVISLVTQKPSAWEF
                     DVEHVTVLNAGVIILLQLFVSRIVSRFRALPTIITGITLGTAGMAILSISANAWVFIT
                     GCIVFTLGEMTAHPKFNSFVGLIAPSGKKAIYMGYSFFCSVIGSFVGGFVGAPLYRKF
                     VDNMGRPDLLWLIFTCVGLLSVAGLIWFNRFVIKKGTFRS"
     gene            1826..3757
                     /gene="rpsA"
                     /locus_tag="PLM68_05130"
     CDS             1826..3757
                     /gene="rpsA"
                     /locus_tag="PLM68_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOT54782.1"
                     /translation="MAKEKELSPKNNLNDVQDPEEIIIREKEKAEDYSEEAPEVDVLK
                     KKAINNASIPVEQFDWAAFEKENVYDTEKSVIEDQYVQTLNKVIENEIVEGTVVALNK
                     REVIVNIGFKSEGVISINEFRYNPELAVGEKVDVYVETAEDRKGQLILSHKKARSLRS
                     WDRINEAYNKDEIIKGYIKCRTKGGMIVDVFGIEAFLPGSQIDVKPIRDYDVFVDKTM
                     EFKIVKINHEYRNVVVSHKALIEAELEAQKADIIGKLEKGQILEGTVKNITSYGVFVD
                     LGGVDGLIHITDLSWGRINHPDEIVQLDQKINVVILDFDDTKKRIALGLKQLTPHPWD
                     SLGDNITVGTVVKGRVVVIADYGAFVEVQPGVEGLIHVTEISWSSHLRSAQDFLKVGQ
                     EVDAVVLTIDKEERKMSLGIKQLKPDPWSYINEKYPVDSRHTAMVRNFTNFGIFVELE
                     EGVEGLVHISDLSWSKKVKHPSEFTSIGETLDVVVLEVDQDNRRLRLGHKQLEENPWE
                     VFETVFTNGSVHEGTVTNFVDKGATVTLPYGIECFVGQKNLVKEDGSAVVLEEKLPFK
                     VLEFNKNSKRITISHTRTWEQEPKKAAAVKEKPETEAETTQRAVKKLKANLEKTTLGD
                     ISELAALKSEMEQNATDTD"
     gene            3738..4544
                     /locus_tag="PLM68_05135"
     CDS             3738..4544
                     /locus_tag="PLM68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOT54783.1"
                     /translation="MPLTLTSLGSASALPARNTYFTAHVLNIRGRSFLLDCGEGTQLR
                     LGQLGISLGAIDHVFITHLHADHVFGLLGMISSMHLLKRNAPLYVHAPRGLEKLIEFY
                     FDFSGKQDPSFPIIHKVVEGTGMFPVMDGGEALVSAFPLNHGVPTFGYLFREKRVRKS
                     EPRSVAYCTDTAPFPELAGWVEGVDLLFLEATFLEKDLESAQNYKHCTARQAAQIALD
                     AGVGKLLLGHFSSRYKEAPAYLAEAREIFPDTELSTESRTFSVPRRLIAT"
     gene            complement(4531..5781)
                     /locus_tag="PLM68_05140"
     CDS             complement(4531..5781)
                     /locus_tag="PLM68_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOT54784.1"
                     /translation="MKETKQPWWKKILTEDWITVIAATIILLLAVIVPAVMPVMPKNL
                     GTGKEWLDAGYMFLFLLLVTYLTSFILKKPLKGIFLSFLAIYILALLSNVVAAIPAIK
                     AIGLESVFFAVIFGLIISNIFNVPKWLKPAIQSEFYIKIGIITLGSTILFGEVMKAGA
                     YGLVQAILIVLVVWNFAFWVARKMKVDDEMATMLASGVSICGVSAAIATCGVIKGDNK
                     KLSTVISLVLVIAIPMMYLLPWLSNLIGLSPQVAGAWLGGTIDTTGAVAAAGTMLGEE
                     AAQTAIIVKSSQNVLLGIAAFLIALYWTYKGKEGQEKPTGKMIWDRFPKFVLGFILAS
                     LIFSIFFSIEEAKVVGKITKGFSNSLFSIAFVCIGLETRFKEIFSKENRRPLNAFLTA
                     QTFNIILTLIVAYLLFGVIQPMLQ"
     gene            complement(5814..6827)
                     /locus_tag="PLM68_05145"
     CDS             complement(5814..6827)
                     /locus_tag="PLM68_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT54785.1"
                     /translation="MKKIILTISFLATVLFSTMQAQAQVSDLEERTGKLESIIAKLPK
                     MSGFINLRYQYEDGANSFDIRRARLDFKGEPAKWFDYRLQLDFASSPKIIDAFVRLKL
                     HPMFNVQAGQFKVAFSLENPYGPLDLETIENSQVVGYLSGFNDLAGNKATGRDIGVAA
                     YGGFFQQDGYNIVDYFVGLYNGTGINVKDNNKHKDFAGRININPIRPVTLSAFYYNGK
                     IGPDGELAPRTRFGFGARYDDGKILFRGEYIKGKTNQKDSDGFYLLAGYTIADKFQPL
                     FKYDYMRLDLADENSRQINYLVGIDYWPHKMFRLQVNYTFRTFIQKEKNSGLLGIMLT
                     ARF"
     gene            6953..8545
                     /locus_tag="PLM68_05150"
     CDS             6953..8545
                     /locus_tag="PLM68_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT54786.1"
                     /translation="MKRISANGNKTLLTILFVFLWLPLGAQETTNDLNQQVSKLGQAL
                     FYIRQYYIDTVDAKRAVDEMITTLTAQLDPHSSYIPEEDVRSMNEPLEGNFEGIGIEF
                     SIIRDTLVVMTPVAGAPSEAVGIRADDRIVAVNGEEISSPELTNQKVFSLLRGPKGTR
                     VSLTVVRRGETGPLNFEVVRDKIPIHSVDAAYWAAPGVMYVKLNKFALTTQDEFIDAF
                     KDLDKMPQGLILDLQGNGGGYLGAALFMAEQFLAKGELILYTEGAKMQRRDVQASGMG
                     FFINTPVVVIMDERSASASEIVAGAIQDWDRGIIVGRRSFGKGLVQQLFPMNDGSELR
                     LTVARYHTPCGRVIQTPYVMGEQERYYRDFLERYERGESFSQDSVQIPDSLAFRTLKK
                     NRIVYGGGGIMPDVFVPADTSYYSGFFIQLARRGIVNAYVNDYLDREREKLAKQYLTF
                     EAFDRQVSLEDMPFEGLTAYAATFGIVPGEGDLALSENHIRMQIKALIASRLFGTACF
                     YRVINPVLPEYRKALEVMNGLL"
     gene            complement(8526..9623)
                     /gene="mnmA"
                     /locus_tag="PLM68_05155"
     CDS             complement(8526..9623)
                     /gene="mnmA"
                     /locus_tag="PLM68_05155"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT54787.1"
                     /translation="MMKIAIGLSGGVDSSVAALLLRRQGHDIFGLFMRNWDDTTGTLQ
                     GHCTWEEDLDLAKMVARKLDIPFFFTDLSEAYRSRVAEYMFREYGKGRTPNPDILCNR
                     EIKFDAFLDKAFELGAQKVATGHYCRIDRTSEGLFRLLQGVDKNKDQSYFLCQLNQDQ
                     LSKALFPIGKLTKAQVRALAREAGLPTSERKDSQGICFVGKVDLPVFLQQKLAPRTGN
                     VVEIFRDGSSKIIGEHQGAHFYTIGQRKGLHIGGHENPLFVLSTDTVSNTVYVGEGQN
                     HPGLYHGNLFIEESDVHWVRPDLAMEQGQERDYSIRIRYRQPLQDGTLHCDGGMHIRF
                     REPQRGITPGQFAVWYQGEELAGSGVIASRS"
BASE COUNT         2558 a         2278 c         2445 g         2345 t
ORIGIN      
        1 cattggcctg cagtgaagcg aacagttttt caacctgact gtaagtcatg cagtccccgt
       61 caatgccggt tacagatcct gcaggcaggt tttcagccag ccattgcggg taatccacgg
      121 catgggggac ccttaacgga tgtatttcga attcggttcc ttccagttct tccctggcct
      181 ggataaaata acgtgtatct gtccagagac ctgcatgggc atgggtaata accactgttc
      241 cgtatgagcc ggtaaaaccg gatatccact ttctctgctg ccaccgttcc ggaaggtatt
      301 cgctcccgtg cgggtccgtt ccggaaatta tggctgcgtc ccatcccagc tgcagcatct
      361 ggtggcggag atcttttatt ctttcagaaa tattcatgac cggaaggttc cttttttaat
      421 tacaaaacga ttgaaccata tgagtcctgc aacactcagc aggcctacac aggtaaagat
      481 cagccagagc aaatcgggcc ttcccatgtt gtcaacaaat ttcctgtaaa gaggagctcc
      541 cacgaaccct ccaacaaaac tcccaatcac actgcagaaa aaagaataac ccatataaat
      601 ggcttttttc ccggatgggg caataagtcc cacgaatgaa ttgaatttgg gatgagcagt
      661 catttctccc agggtgaaaa ctatgcaacc ggtgataaaa acccatgcat tggcagaaat
      721 ggacaggatg gccatacccg ccgttcccag cgtgatgcct gttatgatgg tgggaagcgc
      781 cctgaagcgg cttactatcc gggacacgaa cagctgcagc agaatgatca ctcctgcatt
      841 gagcacggtc acgtgctcca cgtcaaattc ccaggcagaa ggtttctgtg tcaccaggct
      901 gatgacggca ttcactgtat cattaaccgg ggtcatgtcc atgtattcat tcacgtacca
      961 gaggactgtc ccgtacatct gaaagtacat gatccagaaa ccggaataga gcaggatcat
     1021 gacaatgaat ttccagtctt tgaaaaccaa tgccatgtca gagaatattt ttgaaaaagg
     1081 aaccttctcc cttgcccgga ccggttccct gtacaaaaaa atgttcataa gtagcaaaat
     1141 aaccccggca gcagccgcca tccagaaaac gtagctgtag gattcttttt tcagcacggg
     1201 tactaaaagg agcgggaaga ggaaggcacc caggtttatg gaccagtaaa agatgccaaa
     1261 acccagggtg gagttctttt cagtggttga gtaggcaatg gttccggaaa tgacaggttt
     1321 gaaaaagcct gctccgatgg ccatgacaac cagtgaagcg aataccattc cgtaggatgt
     1381 catgaatcct gtcagaaaat atccggtggt cagaaagatg aaagcgacca tgagtatgtt
     1441 cctgaacccg taccggtctg ccagcgcccc tgcaaaaagg ggaagaaaat acaatagggg
     1501 agggatggtc cccatgatgg cgccggccgc ttccctggaa aagcccagtc ctccttcatc
     1561 gggggaaaga acaaggtaaa cggaaagtat ggagagcacc ccgtaatagg caccgcgctc
     1621 aaaaaattcc atgctaatgg ctgtccagaa atttcgtgga aaggatctga tcccggattc
     1681 aggtttcatc ataaaaaaat atgaataaat ttgacccggt aaagttacat tcttttgata
     1741 attaaaaaat aacgtttact tttgcagccc ctgcaaaggg attatacata aagtctaacc
     1801 ttctaattct tattgaacta ttcacatggc aaaagaaaaa gaattgtctc caaagaataa
     1861 cctgaacgac gttcaggatc ctgaagaaat cataatccgg gaaaaagaaa aggcagaaga
     1921 ttattcggag gaagctcccg aagtcgacgt tttaaagaaa aaagcaatca acaacgcatc
     1981 aattcctgtt gaacaattcg actgggctgc gtttgaaaaa gaaaatgtct acgacaccga
     2041 gaaaagtgtc atagaagacc agtatgttca gaccctgaac aaggtcattg aaaacgagat
     2101 agtggaaggc accgttgtcg ctctcaacaa aagggaagtc attgtaaaca ttggcttcaa
     2161 gagcgaagga gtgatatcca tcaacgagtt ccgctataac cccgaactgg ctgtgggtga
     2221 aaaagtggat gtctatgtgg aaaccgcaga agacaggaaa ggtcaattaa tcctttcgca
     2281 caaaaaggcc cgttccctgc gttcatggga ccgcatcaac gaagcataca acaaggatga
     2341 aatcattaaa ggttacatca aatgccgcac aaaaggcggt atgatagttg acgttttcgg
     2401 tattgaagct ttcctgcctg gatcgcagat agatgtgaaa cccatacgcg attacgatgt
     2461 gtttgtggac aagactatgg aattcaagat agtcaagatc aaccatgaat accgcaatgt
     2521 tgtggtgtcc cacaaggccc tgatagaggc agaactggaa gcccagaaag ccgacataat
     2581 agggaaacta gaaaagggcc agatcctgga aggaactgtc aagaacatca cctcatacgg
     2641 tgtgtttgtt gacctgggcg gcgttgacgg cctcatccac attacagacc tttcatgggg
     2701 aaggatcaac catcccgatg aaatcgtaca gcttgaccag aagatcaacg tggtcatcct
     2761 tgactttgat gacaccaaga aacgtattgc cctgggcctg aagcaactta caccgcatcc
     2821 ctgggattcg ctgggagata atatcaccgt gggcacggtc gtcaaaggcc gcgtggtggt
     2881 catagccgat tacggcgcct ttgtagaagt gcagcccgga gttgaaggcc tgatacatgt
     2941 taccgagata tcctggtctt cacaccttcg cagcgcacag gactttctga aagtcggaca
     3001 ggaggtggat gccgttgtgc ttaccattga caaggaagaa cggaaaatgt ccctggggat
     3061 caaacagctc aaacccgatc cctggtctta catcaatgaa aaatacccag ttgattccag
     3121 gcataccgcc atggtcagga atttcaccaa tttcgggatc ttcgtggaac tggaagaggg
     3181 ggtggaaggt ttggttcaca tcagcgacct gtcatggtcc aaaaaggtaa agcatccttc
     3241 ggaattcacc tccatagggg aaaccctgga tgtagttgtt ctggaagtgg atcaggacaa
     3301 ccgcaggctt cgcctgggtc ataagcaact ggaagaaaat ccctgggaag tgtttgaaac
     3361 ggtattcacc aatggctcag tccacgaagg taccgtaacc aattttgtgg ataagggagc
     3421 aaccgttacc ttgccttacg ggattgagtg ttttgtagga cagaagaacc tggtgaaaga
     3481 agatggttca gctgttgtcc ttgaagaaaa gcttccattc aaggtgctgg aattcaataa
     3541 aaacagcaag cgcataacca tatcccacac ccgtacctgg gaacaggaac ccaaaaaggc
     3601 agctgctgtc aaggaaaagc ccgaaacaga agctgaaact acccagagag ccgtcaaaaa
     3661 attgaaagcc aacttggaaa aaacaacatt gggagacatt tccgaactgg ctgcccttaa
     3721 atcggagatg gaacagaatg ccactgacac tgactagcct ggggagtgca tcggcactgc
     3781 ctgccaggaa tacttatttt accgctcacg tattgaacat acgtgggcgg tcctttttac
     3841 tggattgcgg cgagggcacg cagttgcgcc tcggacagct tggtatttcg ctcggggcga
     3901 tagatcatgt ttttatcacg catttgcatg cagatcatgt ttttggtctg ctggggatga
     3961 tatcttccat gcatttgctg aagaggaacg ctccattgta tgtgcatgcg ccccggggcc
     4021 tggaaaagct cattgaattc tattttgatt tttccggaaa gcaggacccg tcatttccca
     4081 tcatccataa agtggttgag ggaacgggga tgtttccggt gatggatggc ggcgaggccc
     4141 ttgtctcggc tttcccgctg aaccacggag tgcccacttt cggttacctg ttcagggaaa
     4201 agcgcgttcg caaaagcgaa cccagatccg tggcctattg taccgatacg gcaccgttcc
     4261 ccgaactggc aggatgggtg gaaggggtgg acctgctctt cctggaggcc actttcctgg
     4321 aaaaagacct ggaatcggcc caaaattaca aacactgcac agcccgccag gcagcacaga
     4381 ttgctctgga tgccggggtg gggaagcttc tgctggggca tttttcatcc aggtataaag
     4441 aagccccggc atatcttgcc gaggccaggg agatttttcc cgatacggag ttaagcactg
     4501 aaagcagaac cttcagcgtt ccgcgcaggc ttattgcaac ataggctgga tcacgccaaa
     4561 gagaaggtaa gcgactatca gggtcaaaat gatattgaaa gtctgagccg tcaggaaggc
     4621 attcagtggc cggcggtttt ctttggagaa tatttctttg aaccttgttt ccagaccaat
     4681 gcacacaaaa gctatgctaa acagggaatt gctgaatcct ttggtgatct ttcccacgac
     4741 tttagcttct tctatgctga aaaaaatgct gaaaatcagg gatgccagta taaatcccag
     4801 cacaaatttc gggaaacggt cccagatcat ctttccggta ggtttttcct gtccttcctt
     4861 tcccttgtag gtccagtaaa gggcaatcag aaaggcagct attcccagca atacgttttg
     4921 tgaggatttc acgatgatgg ctgtctgtgc cgcttcttct cccagcatgg ttccggcggc
     4981 agctacggcg ccagtggtgt ctatggtccc tccaagccag gctcccgcta cctgcgggct
     5041 tagtccaatc aggttggaaa gccagggaag caggtacatc atggggatgg ctatcaccag
     5101 caccagtgag atcacggtag agagcttctt attgtctccc ttgatgacac cgcaggtggc
     5161 tatggcagcg gatacaccgc atatggacac cccactggcc agcatggtgg ccatctcgtc
     5221 atcgactttc atcttgcggg ctacccagaa ggcgaaattc cagaccacca ggacaatcag
     5281 tattgcctgt accagtccgt aggctccagc cttcatgacc tctccgaata aaatggttga
     5341 acccagggtg atgatcccga ttttgatata gaattcactc tggatggcag gcttcagcca
     5401 tttggggaca ttgaagatat tgctgatgat gagtccaaag atgaccgcaa agaaaaccga
     5461 ctccagtcca atggccttaa tggcaggtat agccgccacc acattggata acagggccag
     5521 tatgtatatg gccagaaaag acaggaaaat ccctttcaat ggctttttca ggatgaagga
     5581 ggtaaggtag gttaccagca gcaggaacag gaacatatag cctgcatcaa gccattcttt
     5641 gcctgttccg aggttttttg gcatgacggg catgacggca ggaacgatca cggccagcaa
     5701 caggatgatg gtggctgcaa tcacggtgat ccagtcttca gtcaggattt ttttccacca
     5761 gggttgttta gtttctttca tgataggttg attcatttat tgaataatcg ttgtcagaag
     5821 cgtgctgtta gcatgatacc cagcaacccg ctgttttttt ctttttgaat gaaagtccgg
     5881 aaggtgtagt tgacctgaag ccggaacatt ttgtgaggcc agtaatcgat tcccaccaga
     5941 taattgatct gtctggaatt ctcatcggcc aggtccagtc gcatgtagtc gtatttgaac
     6001 aacggctgga atttgtccgc aatggtatat ccggccagca ggtaaaaacc gtcgctgtcc
     6061 ttctggtttg tctttccttt gatgtattca cccctgaaca ggatctttcc atcgtcatag
     6121 cgggcaccga acccgaaacg ggtcctggga gcaagttcgc catcgggtcc gattttgccg
     6181 ttataataga aggcagaaag ggtgacaggg cggatgggat tgatgttgat gcgcccggca
     6241 aaatccttgt gtttgttgtt gtccttgaca ttgatgccgg taccgttgta caggcctaca
     6301 aaataatcca ctatattgta tccatcctgt tggaagaaac ctccatatgc cgccacaccg
     6361 atatcgcgcc cggttgcctt gttgcccgcc aggtcattga atcccgaaag gtaacctacg
     6421 acctgtgaat tctcgatggt ttccaggtca agagggccgt acggattttc cagggagaag
     6481 gcgactttga actgccctgc ctgcacattg aacatgggat gcaacttgag ccttacaaac
     6541 gcatctatga tcttaggaga tgatgcaaag tccaactgca aacggtaatc gaaccatttg
     6601 gccggctctc ccttaaaatc caatcttgca cgtcggatat caaaggaatt ggctccgtct
     6661 tcgtactgat atctcaggtt tatgaaacca gacatttttg gaagtttggc tatgatactt
     6721 tccagttttc cggtcctttc ctccagatca gaaacctgtg cctgagcctg cattgtgctg
     6781 aaaagcacgg tagccagaaa tgagatggta agaataattt ttttcatcgg attaattact
     6841 tttattagta tatgggtcga tagtatacac aaatatagtc aaagctttta ctaaaaaaca
     6901 agacttcttc taaaaatggt gtaaagggga gtatttttgt acatttgcaa tcatgaaaag
     6961 gataagtgct aacggtaaca aaacgttgtt aaccatactc tttgtgttcc tgtggctgcc
     7021 tttgggtgcc caggaaacaa caaacgacct gaaccagcag gtgagcaagc tgggacaggc
     7081 attgttttat ataaggcaat attacatcga tacagtggat gctaaaagag ccgtggatga
     7141 aatgatcact actctgacag cccagctgga tccccattct tcttatattc ccgaagaaga
     7201 tgtccgttcc atgaatgaac ccctggaagg taattttgaa ggtataggga ttgaattttc
     7261 aataatccgt gatacgctgg tggtgatgac acccgttgcg ggagctcctt ccgaagccgt
     7321 agggatccgc gccgatgaca ggatagttgc cgtgaacggg gaagagattt cttctcccga
     7381 actgaccaac cagaaggtgt tcagcctgct gaggggccct aagggaacca gggtttccct
     7441 gaccgtggtc cgcaggggag agacgggacc tttgaatttt gaggtggtcc gtgacaagat
     7501 ccccattcac agcgtggatg cagcctattg ggctgccccg ggtgtgatgt atgtcaagtt
     7561 gaacaagttt gccctgacaa cgcaagatga gttcattgat gctttcaaag acctggataa
     7621 aatgccgcag ggactgattc tggacctgca ggggaacggc gggggctacc tgggggcagc
     7681 ccttttcatg gcggaacaat tcctggccaa gggcgagctg atcctttata ccgaaggagc
     7741 gaagatgcaa aggcgggatg tgcaggcttc cgggatggga ttctttatca atacgcctgt
     7801 tgtggtgatc atggatgaaa ggtcggcatc ggctagtgaa attgttgccg gggccatcca
     7861 ggactgggac aggggaatca tagtgggccg ccgctctttc ggtaaaggcc tggtgcaaca
     7921 gctttttccc atgaatgacg gatcggagct gcgcctgacc gttgcacgtt accatactcc
     7981 atgcgggagg gtgatccaga ctccttacgt aatgggtgaa caggaaagat attacaggga
     8041 tttcctggaa cggtacgaaa ggggggaatc tttcagccag gatagtgtgc agatacccga
     8101 ttcactggct ttcagaacgt taaagaagaa ccggattgtg tatggcggtg gcggtatcat
     8161 gcccgatgtt tttgttccgg ccgatacaag ctattactcc ggttttttca ttcaactggc
     8221 aagaaggggc attgtcaatg cctatgtgaa cgattacctg gacagggaaa gagagaagct
     8281 ggcgaaacaa taccttacat ttgaagcctt tgaccgccag gtctccctgg aagacatgcc
     8341 gtttgaaggt ctgacagcat atgccgccac ttttggcatt gttccgggag aaggagacct
     8401 tgccctttct gaaaatcata taaggatgca gataaaagcc ctcatagcat ccagactctt
     8461 tggaacagcg tgtttttacc gggtgataaa ccccgtcctg ccggagtaca gaaaagcgct
     8521 ggaagttatg aacggcttgc tataacacct gagcctgcca gttcttcgcc ctggtaccag
     8581 acggcaaact gacccggagt gatgccccgt tggggttccc tgaagcggat atgcataccc
     8641 ccgtcacaat gcagggtgcc atcctgcagg ggctgccggt agcggatgcg gatactgtag
     8701 tcacgttcct gtccctgctc catggccagg tcgggcctga cccaatgcac gtcactctcc
     8761 tcaatgaaaa ggtttccgtg gtacaatccg gggtggttct gaccttctcc cacatagact
     8821 gtattgctca cggtatctgt tgatagcaca aacagcgggt tttcatgtcc cccgatatgc
     8881 aggcctttcc tttgtccgat ggtataaaaa tgggctccct ggtgctcgcc gatgattttt
     8941 gaggagccat cccggaagat ttctaccaca ttacctgtgc ggggtgccag tttttgttgc
     9001 aggaaaacgg gcaggtccac ttttcccaca aagcagatcc cctgtgaatc ctttcgttcc
     9061 gatgtgggaa ggccggcttc acgggccagt gccctcacct gtgccttggt taatttccct
     9121 atgggaaaca aagccttgga taactggtcc tggttcagct ggcacaggaa ataggactgg
     9181 tccttgttct tgtcaactcc ctgcagcagg cggaacaatc cttcacttgt cctgtctatc
     9241 ctgcaatagt gacctgtcgc caccttttgg gctcccaatt cgaaggcttt atccagaaag
     9301 gcatcgaact tgatttcgcg gttgcacagg atgtcaggat tgggtgtcct gccttttcca
     9361 tattcccgga acatgtattc tgcaacccgt gaacggtacg cttcgctcaa gtctgtgaag
     9421 aaaaagggga tatccagttt acgtgccacc atttttgcca agtccaggtc ttcttcccag
     9481 gtacagtgac cttgcagcgt ccctgtggta tcgtcccagt tgcgcatgaa gagtccgaat
     9541 atatcgtgac cctgccgcct gaggagaagg gcggcaaccg aggagtcaac cccgcctgaa
     9601 agaccgatgg caattttcat aattga
//
LOCUS       DAOSOF010000069         9452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7218_length_9452_cov_6.097797, whole genome shotgun sequence.
ACCESSION   DAOSOF010000069 DAOSOF010000000
VERSION     DAOSOF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7218_length_9452_cov_6.097797"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1166
                     /locus_tag="PLM68_05160"
     CDS             141..1166
                     /locus_tag="PLM68_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOT54788.1"
                     /translation="MKTSFGSDNHSGVHPSVFKALEDANRDYQLSYGEDEYSSRAEDV
                     FRKLFGKDARVFFVFNGTGANVLCLQALTMPYHAIICAETAHINQDECGAPERFTGCK
                     MITVDARDGKLDPSLVKPLLKGFSFQHHSQPKVISISQVTELGTVYTPEEIRRLADLA
                     HEHNMYLHMDGSRLANAAAYLGLPLEEMTTRAGVDALSFGGTKNGMLMGEAVVLLRPH
                     LAENFKYIRKQSMQLFSKSRFVAAQYLAYLEGGLWLENARHANKMAGYMAECIKKLPG
                     VTITQQVESNAVFAIIPEKARKKLVEKHFFYTWNEHTGEVRWMCSFNTLKSHIDSFIN
                     DLKITLS"
     gene            1163..2941
                     /locus_tag="PLM68_05165"
     CDS             1163..2941
                     /locus_tag="PLM68_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT54789.1"
                     /translation="MNTIVPVTRLFDLLERLQVNYPKEDILAGKVDGVWKKYSSQEYA
                     EISHAFAYGFLEFGLQREDKVITVMNNRPEWNFVDMGTALAGVVHVPVYSTLSDDDYR
                     YIIPHSDAKIIIIGSAILYKHIAPVIAGLENPPTVFTIDNVEGVNNLDQIRRMGVENK
                     EKWAPVIEENKKTITPDTLLSIIYTSGTTGTPKGVMHAHSSIVFNFLAHAAQQIKNSS
                     HRYLSFLPLCHIYERTMNYEFQSLGISIYYGENMGTIARDLKDIQADGFCAVPRVIEM
                     MYKKLDDIGKSLTGFKKIIYRWAWNFGNTFDYENLTFRRRLLNSLFDKLVYSKWRDNL
                     GGHEMLIVTGGSSIRACVIRLFSAAKMYIYEGYGLTETSPVIAVNKPKALLRKIGTVG
                     TILEGTEVSFLQDGEILTRGPHIMKGYYKDPEATAEVIDKEGWFHTGDVGEMVNGAYL
                     RITDRKKEIFKLSNGKYVAPQVIEGKLNESPYIENSLVIGSNQKVASAIIVPNAARIE
                     EWATHHKMVFQDAEKLYQTKEVMDLIHKEIERINKTLAPHEQIKFFRLVNDEWTTANG
                     LLSQTLKLRRAQLNKHYDKIIAEIYS"
     gene            2938..3657
                     /locus_tag="PLM68_05170"
     CDS             2938..3657
                     /locus_tag="PLM68_05170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54790.1"
                     /translation="MIRQATAEDRAALLDIWCAAFGDSPDYAAFVIDHCLELGTVLFH
                     PRGMSCMTLFPLTLFSGNKKLLEGFYVYGVVTHPQQQKMGFASLLLDRARTLAPFLLL
                     YPATQPLQEYYKKRGFDTPVRLPAPLIRTATGKEHSGAPVRALYQEYKKDAEKQEFIF
                     VWPFSMFHYAYQECRFRSGYVLPPHFCYPSEKENNVVMCKPYAGGGNNDKALFLQALA
                     HFHDPVPGFITEKSLFYLPLD"
     gene            complement(3661..6024)
                     /locus_tag="PLM68_05175"
     CDS             complement(3661..6024)
                     /locus_tag="PLM68_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54791.1"
                     /translation="MKHFSLFTLICLFSLVTLNAQQMVQTVKGRVVDDIASQPLVGVG
                     VVVNNSGYLATYTDIDGYYNIPNVPVGRISILFTSIGMETVYMENISLNSGKELVINV
                     SMREDVMTVEEIVITAERDKLRPVNEMASVSARTFSVADAERYAGAMSDISRMAQNFA
                     GVGTPNDSNNDIVVRGNSPFGLLWRLEGVDIYNPNHFADGGATGGAISMLNVNTLSNS
                     DFYTSAFPAEYGNAYSGVFDVHLREGNYDKHEFTGQLGINGMEFGAEGPFSRKSKASY
                     LGAYRYSFLDALTYLGFEFGTGSAVPRYQDWTAKINVPTAKSGTFSLFSIGGRGKISI
                     VDGESDFYKYADDIATQYGMAVIGLRYQKSIGHNQSINVSVAGSYSSLSAQIDSMNAE
                     TLLKDRSQDALLQREFQSIQAVHNVKLGNRLSMRSGITGKRLAYKFISNDYSMTTYPK
                     DVNEVGYTFLLSAFSEVSYRAGARLTLDAGVNAQFLFLNQTWNIDPRFGLSWKFKDKH
                     EITFGYGLHSQYQGLEIYMTKLFSQAVDSKIYPNRYLDMTRAHHLVLGYQWRIAPSTR
                     LKAETYFQYLYNIPVDLYEPYYSVVNLTGMSFDKYGRVYVNDATGYNYGLELTLERFL
                     SQGWYYMVTASVFESKFRSIDNILRNTLYNSNFVSNILVGKEFELTKKAQARNIWSFG
                     GDIKFAFAGGQRYIPVDLEASKATGTTVYIYEQAYVPRHPFYMRGDLKAWAKIHQQKV
                     THEIGVEIRNFTNRKNVYSYEYDVKLKDMKTSYQTGLLPLGYYRITF"
     gene            complement(6164..8041)
                     /gene="mnmG"
                     /locus_tag="PLM68_05180"
     CDS             complement(6164..8041)
                     /gene="mnmG"
                     /locus_tag="PLM68_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT54792.1"
                     /translation="MTNRYDIIVVGAGHAGCEAAHAAARMGSKTLLITMDMTKMAQMS
                     CNPAVGGIAKGQIVREIDAMGGVMGIVTDASTIQFRMLNRSKGPAMWSPRAQCDRMLF
                     SLKWREILEKTENLYMWQDQVLSLLFDKNKVTGVATEFGQEFFAEAVILTNGTFLNGK
                     IFIGWKTTPGGRMGEQPSLALSKQLKERGIQVERMKTGTPPRVDGKTIDFSHLEIQRG
                     DEKPQKFSFLTTQSAIQERNQQLPCYIGYTNTDVHEKLKEGFNDSPLFAGRISGKGPR
                     YCPSIEDKLRTFEHKEQHPLFLEPEGWHTNQYYIQGFSSSLPIDIQYQAVRKVKGLEN
                     AHFHSPGYAIEYDYFPPTQLDHSLEVKNIPGLYFAGQVNGTTGYEEAAAQGLMAGINA
                     HRKIHKLQPIILHRDQAYIGVLIDDLITKGVDEPYRMFTSRAEYRILLRQDNADERLT
                     LIGFNIGLATEERHSLLTEKIMKRDRLLQYLESNSVDPDKINDMLQKVGSAPIETKKK
                     MAQILSRPQVEWGDMATFVPRGTLCEDDPRAAEIYESVEIVLKYRGYIEREKAFADKV
                     LRLDSVVIPEKFDYTRIVSLSTESRQKLLRVKPRTIGQASRIPGVSPADISVLLLYLG
                     R"
     gene            complement(8038..8472)
                     /gene="ybeY"
                     /locus_tag="PLM68_05185"
     CDS             complement(8038..8472)
                     /gene="ybeY"
                     /locus_tag="PLM68_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT54793.1"
                     /translation="MILFHTNQTNINISNRRAIKSWIGDLLQKYGKTPGEIGIIFCTS
                     EYMLDLNKRFLKHDNYTDVITFNYSDNKEKRMSGDIFIDHETVFYNAARFGQEKHVEI
                     LRVIAHGLLHMLGYDDDTEESRDNMHRLEDNALKEIQINPLG"
     gene            8547..8620
                     /locus_tag="PLM68_05190"
     tRNA            8547..8620
                     /locus_tag="PLM68_05190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8581..8583,aa:Ala,seq:ggc)
     gene            8642..9334
                     /locus_tag="PLM68_05195"
     CDS             8642..9334
                     /locus_tag="PLM68_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT54794.1"
                     /translation="MELKKSPKANLENKRMLFIEIGLVVALVIVLAAFEYKTYEKNAS
                     FLDAEVQQVIEEEQIPVTLETPPPPPETPQAPILSDAIEIVEDDITIEDDIIIETEDD
                     AAFAVEIRDYITYTQEETVEEEEIPVAIVEEKPTFLGGDENTFTKWVFDRIVYPEVAK
                     ENGVQGRVVLSFIVDSDGYVKNVTVLRGVDPSIDKEAVRVVSSSPQWKPGRQRDKNVR
                     VRYNFPLNFQLR"
BASE COUNT         2493 a         2231 c         2061 g         2667 t
ORIGIN      
        1 aaattaaatc gccggaccaa taaccttgca ttcatcaacc catgagtgct cttttggctt
       61 ttgcataaac cgaatggttt aaaacataat atattggaaa agaaatacat ggcaaaatta
      121 ctactattaa aattttacgt atgaaaacaa gctttggaag tgacaatcat tccggagtac
      181 atccgtctgt atttaaggct cttgaagacg ccaaccggga ttaccagctt tcttacgggg
      241 aagatgagta ctcttccagg gcagaggatg tgttccggaa attattcgga aaagatgccc
      301 gggtattttt tgttttcaac gggacagggg ccaacgtgct ctgcctgcag gcgcttacaa
      361 tgccttacca tgccataatc tgtgcagaga ccgctcatat caaccaggac gagtgcggcg
      421 ctcccgaacg gtttaccggg tgcaaaatga tcaccgtaga tgcacgggac ggtaaactgg
      481 acccctccct tgtcaaaccc ctgttgaagg gattcagttt ccagcatcat tcccagccca
      541 aagtcatatc catttcccag gtcacagaac tgggtactgt ttacacaccg gaagaaatcc
      601 ggagactggc agacctggcg catgaacaca atatgtacct gcatatggac ggatcccggc
      661 tggccaatgc ggcagcgtac ctgggcctgc cactggagga gatgaccacc cgggcgggtg
      721 tggacgccct ttcctttggc ggcacaaaaa acggaatgct catgggggaa gctgtcgtgc
      781 tgcttcgccc ccacctggct gaaaatttca agtacataag aaaacagtcc atgcagcttt
      841 tttccaaaag ccgttttgtg gcagcacaat acctggcata cctggagggc ggcctatggc
      901 ttgaaaacgc ccgtcatgcc aacaaaatgg ccggatacat ggccgaatgc ataaagaaac
      961 tgccgggtgt gaccatcaca caacaagtgg aaagcaatgc cgtttttgcc attatcccgg
     1021 aaaaagcccg caagaagctt gtagaaaagc acttcttcta cacctggaat gaacatacgg
     1081 gggaggtacg ctggatgtgc tccttcaata cccttaaatc acatattgat tcatttatta
     1141 acgatttaaa aataacacta tcatgaatac aatagtacca gtcacccggc ttttcgacct
     1201 gctggagcgt ttgcaggtga attatcccaa agaggacatc ctggcgggta aagtagatgg
     1261 cgtctggaag aaatacagct cgcaggagta tgccgaaatc tcacacgctt tcgcttatgg
     1321 tttcctggaa ttcggcctgc aacgggaaga caaggtgatc accgtcatga acaaccgtcc
     1381 ggaatggaat tttgtcgata tgggaacagc cctcgcagga gttgtccatg tgcctgttta
     1441 ttccaccttg agtgatgacg attaccgcta tatcatcccc cattccgatg caaaaatcat
     1501 catcataggc agtgcaatcc tatacaagca tattgccccg gtaatcgccg gactggagaa
     1561 tcctccgaca gtgtttacca tagacaacgt ggaaggcgta aacaacctgg accagatcag
     1621 gcgtatggga gttgaaaaca aagaaaaatg ggcccctgta atagaggaaa acaaaaaaac
     1681 aatcaccccc gacacccttc tttccatcat ctacacatcg ggcaccacgg gaacgccaaa
     1741 aggcgtgatg catgcccaca gcagcattgt ctttaatttt ttggcacatg ccgcgcagca
     1801 gatcaagaac tcatcccacc gttacctcag cttccttccc ttatgccata tatatgaaag
     1861 aaccatgaac tatgagttcc agtccctggg aataagcatc tattacgggg aaaatatggg
     1921 gactattgcc cgtgatctga aagacatcca ggcagacggt ttctgtgctg ttccgcgtgt
     1981 cattgaaatg atgtataaaa aactggatga tataggcaag agccttacag ggttcaaaaa
     2041 gattatctac agatgggcct ggaatttcgg gaacactttt gattatgaaa atctaacctt
     2101 taggcgcagg ttgcttaaca gtttgtttga caagctggta tatagcaaat ggagggacaa
     2161 cctgggcggg catgaaatgc tcattgtgac cgggggctct tccatcaggg cttgtgtcat
     2221 ccgtttgttt tcagcagcca aaatgtacat ttatgaagga tacgggctta cagagacttc
     2281 cccggtgatt gccgtcaaca agccaaaagc cttgcttcgc aagataggaa ctgttggtac
     2341 gatactggaa gggacagagg tatcattcct gcaggacggc gagatcctga cccgcgggcc
     2401 gcatatcatg aagggatact acaaggaccc tgaagccaca gcagaagtga tagacaagga
     2461 gggctggttc cataccggcg atgtgggtga aatggtcaat ggcgcttacc tccgcattac
     2521 agaccgtaaa aaggagatct tcaagttgtc caatggaaaa tatgtggcac ctcaggtgat
     2581 cgagggcaaa ctcaacgaat ctccgtacat tgaaaactcc ctggtcattg gcagcaatca
     2641 aaaggtggcc tcggccataa tcgttcccaa cgccgccagg attgaggagt gggcaaccca
     2701 ccataaaatg gtgtttcagg atgcggagaa actctaccag acaaaagaag ttatggattt
     2761 gatacacaag gagattgaaa ggatcaacaa gacactggca ccccatgagc aaatcaagtt
     2821 tttccgtctt gtgaatgatg aatggaccac ggcaaacggc ttgctgtcac aaacactaaa
     2881 attacgcagg gcacaactca ataagcatta cgataaaata atagccgaga tctattcatg
     2941 atccggcagg ccacagcaga agacagggcc gcactgctgg acatctggtg tgctgctttc
     3001 ggggacagtc cggattacgc cgcctttgtc attgaccact gccttgagct gggcactgtg
     3061 cttttccatc cccggggtat gtcttgcatg acattattcc cgttgacatt gttcagcggg
     3121 aataaaaagt tactggaagg tttctatgtt tacggggttg taacccatcc acagcaacaa
     3181 aaaatgggat ttgcctctct gctgttggac cgggccagaa cccttgcccc ctttttgtta
     3241 ctgtacccgg caacccaacc tctccaggaa tactataaaa aacgaggatt tgacacccct
     3301 gttcgcttac ctgccccgct tatcaggacg gctacaggta aggaacattc cggtgcccca
     3361 gtaagggcct tatatcaaga atacaaaaaa gacgctgaaa aacaggaatt tatttttgtc
     3421 tggccgtttt caatgtttca ctatgcatac caggaatgtc ggtttaggag cggatatgtc
     3481 ttgccacccc atttctgcta tccgtctgaa aaagaaaaca atgtggttat gtgcaaacca
     3541 tatgccgggg gcggaaacaa tgacaaagcc ctgtttctgc aggctttggc gcattttcat
     3601 gatcctgttc cgggatttat aaccgaaaaa agtttgtttt atctcccttt ggattgaatg
     3661 tcagaatgtt attctataat atcccagggg tagcaggcct gtctgatacg atgttttcat
     3721 gtctttcagc ttcacatcgt attcatagga atacacattc ttgcggttgg tgaaattcct
     3781 gatctctacg ccgatttcat gggtaacctt ttgttgatgg atctttgccc atgccttgag
     3841 gtccccgcgc atgtagaatg ggtgcctggg gacataagcc tgttcataga tgtagacagt
     3901 cgttcctgtc gccttcgagg cttcaaggtc tacgggaata taccgttgcc cgcctgcaaa
     3961 agcgaatttg atatcacctc cgaaagacca gatattcctt gcctgagctt tttttgtcaa
     4021 ttcgaactct ttgccgacca gtatgttgga aacaaaattg ctattataga gtgtgtttct
     4081 caggatatta tcaatgctcc tgaacttgct ctcgaacaca gaggcagtga ccatataata
     4141 ccacccttgt gaaaggaaac gttccagcgt cagttccagc ccgtaattgt agcccgtagc
     4201 atcgttcaca taaacgcgcc cgtacttgtc aaaactcata ccggtcagat tgaccactga
     4261 ataataaggt tcatacaggt ccacaggaat attgtaaaga tattgaaagt acgtttctgc
     4321 cttcaaccgt gtcgagggcg cgattctcca ttggtatccc aggaccaggt ggtgtgcacg
     4381 ggtcatgtcc aggtacctgt tgggataaat cttgctgtcc acggcctgcg agaacaattt
     4441 tgtcatgtag atctcaagtc cctggtactg gctgtgaagg ccgtaaccaa aggttatttc
     4501 gtgcttgtct ttgaacttcc aggaaagtcc aaagcggggg tcaatattcc atgtctgatt
     4561 taggaaaagg aactgcgcat tgacgcctgc atccagggtg agtctggccc ctgcccggta
     4621 agaaacttcc gaaaacgcgc tcaagagaaa cgtatacccg acttcattaa catccttggg
     4681 atacgtggtc atggaataat cattggaaat gaatttgtag gccaggcgct taccagtaat
     4741 gcccgagcgc atggataacc tgtttcccag cttcacattg tgaaccgcct gtatgctttg
     4801 gaattctcgt tggagcagcg catcctggct gcggtctttc aggagtgttt cagcattcat
     4861 ggagtcaatc tgcgccgata aagaagaata cgagccggcc acggaaacgt tgatcgactg
     4921 attgtgccct atgctttttt ggtaccgtag ccctatcacc gccattccgt attgggtggc
     4981 aatatcatct gcgtatttat aaaaatctga ttctccgtcg actatgctaa ttttgcctct
     5041 tcctcctatg gaaaataatg aaaaggttcc gcttttcgcg gtcggaacgt ttatcttggc
     5101 cgtccagtcc tgataccttg ggacagcaga tcctgttccg aactcaaaac ccaggtatgt
     5161 gagcgcatcc aggaatgaat aacggtaagc tcccagatag gaggctttcg atttcctgga
     5221 gaatggtcct tctgccccga attccattcc gttaataccc agttgacctg tgaattcatg
     5281 tttatcatag ttgccttccc tcaggtggac atcaaaaaca ccggaataag catttccata
     5341 ttctgcggga aaagcgctgg tataaaaatc cgagttggat aatgtgttta cattcagcat
     5401 gctgattgcc ccccccgtag ctcctccatc ggcaaaatgg tttgggttgt aaatatccac
     5461 gccttccaag cgccataaca atccgaatgg agaattaccc ctgactacaa tgtcgttgtt
     5521 tgaatcgttc ggtgtgccga ctcctgcaaa attctgtgcc atccttgata tatcgctcat
     5581 agcccctgca tacctttctg catctgcgac tgaaaaagtc cttgccgaaa cggaagccat
     5641 ttcgttcaca ggacgtagtt tgtccctttc tgcggtaatg acgatttcct ccaccgtcat
     5701 cacgtcttct ctcatggaca cattgataac tagctcttta cctgaattta gtgaaatgtt
     5761 ttccatatat acggtttcca tcccgatgct ggtgaaaaga atggagatcc tccccaccgg
     5821 aacattgggt atattgtaat aaccgtcaat gtccgtatac gtggccaggt aaccgctgtt
     5881 attgacgacc acacccacac ctaccaaagg ttgtgatgca atatcgtcca cgaccctgcc
     5941 tttcactgtt tgcaccattt gctgtgcatt gagggtaacc agcgaaaaga ggcagatgag
     6001 ggtgaaaagg gaaaaatgtt tcaaggtgta tatgtgcttt ttgcgtgaac cgacaaaagt
     6061 agtatatttt tttctcttat ttactaaatt atatactcga ataaagtagt ctttattcag
     6121 gtaacattat gtttttgctt gtatattaat aaaatgcatt cccttaccgc cccaaataca
     6181 gcagtaaaac agaaatatcg gcaggagaca ctccgggaat cctggatgcc tgccctatgg
     6241 tccggggttt aaccctaagt aatttttggc gtgattcggt agacagggat actatcctgg
     6301 tataatcaaa cttctcgggt ataaccacgg aatccaatct caacaccttg tctgcaaagg
     6361 ctttttctct ttcaatatat cccctgtact tcaacacgat ctctacagat tcgtatattt
     6421 cagctgccct tggatcgtct tcacataatg ttccacgtgg aacaaaagta gccatatctc
     6481 cccattcaac ctgaggtctt gacaaaatct gggccatctt ttttttcgtt tcaattggag
     6541 ctgaccccac tttctgcaac atatcattaa ttttatcagg atctacagaa ttgctctcca
     6601 ggtattgtag caatctgtct cttttcatga tcttctctgt cagcagggag tgtctttctt
     6661 ctgttgccaa tccaatattg aacccgatta gcgtaagcct ttcatcagca ttatcctgcc
     6721 tgagcaggat tctgtattcc gcgcgtgaag tgaacatcct gtaaggctcg tctactcctt
     6781 tggttatcag atcatctata agaacaccaa tataagcctg gtctctgtgt agtattatgg
     6841 gctgtaattt gtgaattttc ctgtgcgcgt ttatacctgc catcaggcct tgtgctgctg
     6901 cttcttcata tcctgtcgtg ccattcacct gtcctgcaaa gtacaaaccc ggtatattct
     6961 tgacttccaa tgaatgatcc aattgtgttg gtggaaaata atcgtattca atagcgtatc
     7021 ctggactgtg gaaatgagca ttctccaggc ctttgacttt tcttactgcc tggtattgta
     7081 tgtcaatggg taatgaagag gagaatccct gtatgtaata ttggtttgta tgccatcctt
     7141 ccggttccag gaaaagaggg tgttgctcct tatgttcaaa ggttctcaac ttgtcctcta
     7201 tactgggaca ataccttggc cctttcccgc ttatccttcc agcaaacaat ggtgaatcgt
     7261 tgaaaccttc tttcagtttt tcatggacat ctgtattggt atagccgata tagcaaggta
     7321 gttgttgatt tctttcctgt atggcagact gtgtcgtcaa aaagctgaat ttttgcggtt
     7381 tttcatctcc tctttgaatt tccaggtgcg aaaaatcaat tgtcttgcca tctactctcg
     7441 gtggcgtacc tgtcttcatc ctttcaacct gtatccctcg ttcctttagt tgtttgctca
     7501 aggccaggga tggctgctct cccattcttc ccccaggtgt tgtcttccat ccaataaata
     7561 tttttccgtt cagaaaagtt ccgttggtga gaatcactgc ttctgcgaaa aattcctgac
     7621 cgaactctgt tgcaacacct gtcactttgt ttttatcaaa caaaagcgat aatacctggt
     7681 cctgccacat atataaattt tcggtctttt ccaggatttc tctccatttt agggaaaaca
     7741 gcattctgtc acattgtgcc ctcgggctcc acattgccgg cccttttgac ctgtttaaca
     7801 tcctgaactg tatggtgctg gcatccgtaa caatacccat gacccctccc atagcgtcta
     7861 tctctcttac tatttgtcct ttcgcgatgc ctcctacggc cggattacat gacatctgtg
     7921 ccatttttgt catgtccatt gtgataagaa gcgtttttga acccattctt gctgccgcgt
     7981 gcgctgcttc acatcctgca tgacctgccc caactactat aatatcgtac ctgttggtca
     8041 tcctaatgga tttatttgaa tttctttcaa ggcattatct tccagtctgt gcatattgtc
     8101 cctgctttcc tccgtatcat catcgtaccc aagcatgtgc aacagtccat gtgcaatgac
     8161 acgtagtatt tcaacatgct tttcctgtcc gaatctcgct gcattataaa acacggtttc
     8221 atggtctata aaaatatccc cactcattct tttttctttg ttatctgagt agttgaacgt
     8281 tatcacatcg gtataattat catgtttgag aaaccttttg ttcaaatcaa gcatatattc
     8341 tgatgtacag aatattatac caatttctcc cggtgttttt ccatattttt gcagcaagtc
     8401 tcctatccac gattttatgg cacgtctgtt gcttatattt atattggttt ggtttgtatg
     8461 aaacagtatc atggctatgt ataaaaacga tgcaaaatta caaaattgtt ttttaaatta
     8521 ttttttttac atttgtaagt cctgacgggc cattagctca gctggctaga gcgcttgcat
     8581 ggcatgcacg aggtcgtcgg ttcgattccg acatggtcca caaaatttat tcttgaataa
     8641 catggaactg aaaaaaagcc ctaaagcaaa tcttgagaat aagagaatgc ttttcattga
     8701 gatcggcctg gtagtcgctc ttgttattgt tcttgctgct tttgaataca aaacttacga
     8761 aaaaaatgcg agttttctcg atgcagaggt acaacaagta attgaggaag agcaaattcc
     8821 tgtaacattg gaaacacctc ccccgccacc cgaaactccc caggctccta tactttccga
     8881 tgccattgaa atcgttgaag acgatatcac cattgaagac gacattatca ttgaaacaga
     8941 agatgacgct gcttttgcgg tagagatccg cgattacatc acttacacgc aagaagaaac
     9001 tgtagaggaa gaggaaatcc ctgttgccat tgttgaagaa aaacccacat ttctgggcgg
     9061 cgacgagaat accttcacaa aatgggtatt tgatcgcata gtttatcccg aggtggcaaa
     9121 agaaaacgga gtgcagggcc gcgtggttct atctttcatt gtagattctg acgggtatgt
     9181 aaagaacgtt acggttcttc gcggtgttga cccctccatt gacaaggaag ctgttcgggt
     9241 ggtgtcatcc tctccccagt ggaaacccgg aagacaacgc gacaaaaatg tcagggttcg
     9301 ttacaatttc ccgttgaatt tccagcttag ataatcagtg ggttgtaagc ctttgtttgt
     9361 taaagcgcct tatctttacg ggcgcttttt ttttgatcct caattccgca agaaagtttg
     9421 ataatggcag aatgagcgtg tcccagaggc aa
//
LOCUS       DAOSOF010000070         9285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7369_length_9285_cov_4.853738, whole genome shotgun sequence.
ACCESSION   DAOSOF010000070 DAOSOF010000000
VERSION     DAOSOF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7369_length_9285_cov_4.853738"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLM68_05200"
     CDS             complement(<1..678)
                     /locus_tag="PLM68_05200"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOT54795.1"
                     /translation="MIHYLDKYLKTLIQEKELITINRLVDPNLEITEITDRQSSLPHG
                     GKALLFDNNGTSFPVVTNLFNSKKRIALAFGLDNMEDVATRISAFYEAISQYQSGNRF
                     MKKSPAYDLTQYVPRTSRSGSCQEIALFPPDLNRIPFLRNRPFDEAHSLHDVPMIMKN
                     PYMNSYAIESVRILWHSKTTVQIRLEAGSRAAFFVNDSSKGRIPVTLFLGEIRYIRSL
                     VIFHIRQT"
     gene            complement(686..2401)
                     /locus_tag="PLM68_05205"
     CDS             complement(686..2401)
                     /locus_tag="PLM68_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT54796.1"
                     /translation="MWSLFIFLSYSISLCLNGGSNESVILAIQQGEYVVPVDTLNPDH
                     AGCVRFTGNSPLEAGQYVFVQNNKRLFNFLISGQEDITFSFTAHMDGGRTLDVRADGS
                     EENAAYIRFNRFLQEHYSRANEMLDGNRDPEQALAEVQRLESTIREYASFVARQFEGQ
                     MLGIIAGNIFTPSVPPAETPLHYLDHIDFSDSRILNTSILPVRLKEYFTSILPPLPDS
                     LVFHADRILKGNIHPKVKGYVARYLFTLFFTSEIMGVESAAVHMAEKWLLADTSLCPD
                     PDLVREIEIFVRFNKQCLLGMKAPDLKLPDVHSREQSLHDLDARYTIVVFFEDNCPAC
                     SSELLKLTRFAQDHTEDDIHYYAVYTQDNKDILSRYADFFPEDWISVWDPDMSSGFQE
                     KFNVRSTPKIFLLDKDKIIIGRDLNTENLEELLTNQNLKQEPVLRKAPDLILETENGD
                     PCTLYEIEASYTVLYFYDPACSVCGLVTARLYELSRMAADKGMVFFAVYIGNDYLSWR
                     KWLIEGGYTGWINVWNPFDDDRIYKNYNVADPPLVLLLDEEKGIVADNLSTAALSMII
                     NQLKP"
     gene            complement(2409..2885)
                     /gene="coaD"
                     /locus_tag="PLM68_05210"
     CDS             complement(2409..2885)
                     /gene="coaD"
                     /locus_tag="PLM68_05210"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT54797.1"
                     /translation="MAKTAVFPGSFDPFTSGHLDVLISARKLFDKIVVAVGVNQQKVS
                     LFSPVARVAIIREVIKDLPDVEVCSYNGLTIDFCRKNKINFIFRGIRTTTDFELEQVI
                     AQVNSKMAPEIQTIFIPSGPKYSFISSTVVRDVLLNGGDATCMLPPGLDLNKYLTM"
     gene            complement(2949..3617)
                     /gene="ung"
                     /locus_tag="PLM68_05215"
     CDS             complement(2949..3617)
                     /gene="ung"
                     /locus_tag="PLM68_05215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT54798.1"
                     /translation="MDVKIEASWKNVLQPEFSKPYFEGIASFLHDEIRQGKTIYPPGK
                     LIFNAFALTPFHALKVVLLGQDPYHQPGQAHGLSFSVPVTMPQPPSLQTIFTEMRSDL
                     GISTPRHGCLEGWARQGVLLLNAILTVEAGKPASHSKTGWMDFTDAVIRTVSDQKDHV
                     VFLLWGNFARSKRGLIDTSRHCILEAAHPSPLARGAFLGSKPFSATNMYLASKCIQPV
                     DWRL"
     gene            complement(3619..6012)
                     /gene="lon"
                     /locus_tag="PLM68_05220"
     CDS             complement(3619..6012)
                     /gene="lon"
                     /locus_tag="PLM68_05220"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT54799.1"
                     /translation="MNLTLSDKMTVIPLMEMDMDQTSGDMIKDGMPQVLPLLVLRNAV
                     VFPGTLIPITVGREKSIKLVRESLTGDKWVGTVTQMDPLDDDPEENEISDHGCLAKIL
                     KIIEMPEGGITVILHGFRRFKIDRIVQKEPYFAAQVIYLNDIHPAQDDRGYKAVVDGI
                     KDVAAQIFQLTPSIPREAEFALRNIEKDDFLVNFVASNIYLDGIKDKLALLSIDKLND
                     RASRLLELLYKHLELLKFKEQIQQKVRGEIDQQQKEYYLSNQLKTIQDELGLTQDMEE
                     VDTLREKAANKKWSKEIAEIFEKEVQKLERANPHQPDYAIQLNYLQFMVELPWGEYTK
                     DNLDLKRARRVLDKDHSGLETVKERILEHLAVLKLKGDMKSPILCLYGPPGVGKTSLG
                     KSVARALGRNFARISLGGLHDESEIRGHRRTYIGAMPGRIIQSIKRSKTSNPVVVLDE
                     IDKVGTDFRGDPSSALLEVLDPEQNNTFHDNYLDVDYDLSRVLFITTANNIGGIQPAL
                     RDRMEMIPINGYLAEEKRTIALKHLLPKQLEAHGLKPSQIKLGRQSIDVIINEYTRES
                     GVRNLDKQLAKVARHVAKKIAFGEEYDPVLTPENIREILGVHRIIQDVQKGNEFPGVV
                     TGLAWTETGGEILFVETSLSKGKGTLTTTGSLGDVMKESAIIAQQYLKAHADMLGLDP
                     GIFESTDIHLHVPEGAIPKDGPSAGITMICSMASSFLNKCIKKGVAMTGEITLRGRIL
                     PVGGIKEKILAAKRSGIKTIILSEDNRKDIEEIKPDFIKGLEFKFMKTIPEVIKYVL"
     gene            complement(6082..7503)
                     /locus_tag="PLM68_05225"
     CDS             complement(6082..7503)
                     /locus_tag="PLM68_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54800.1"
                     /translation="MKSNPLLRLLFTVCLLLLSHAGWSGNLRIDGILLTGNKVTKDFI
                     ILRELPFSKGDTIPEEVFPDKLRLARENLNNLLLFNFIEISPLRSADDSSLVTVIIQV
                     EERWYIWPLIEVKLEERNLSTWLQSWDFTKITIEAGARIDNFLGLKHKLVIAAHGGYQ
                     WGVFLGYRDISLDRQGKHFMSVDLQGNISHNLDIFTQNDKPARFNIRDIYLEKTIDTR
                     INYTYRPDIRSIHNVSLMHEYTLLADTVLSLNRHYWGNDQLKRNAFRLNYRYRLDHRD
                     YHPYPLNGYFLQAGLNTYITTDASVRYAQVTLGLQYFKQLGASRWYVSSVFSGGYSLS
                     NAQAYILERAIGYDNNMLRGYEYIVADGQSFVIFNNTLKFNILKKRTFTLNWLSFLPK
                     FNKIHLSIYANAHFDAGYARNKWDYHLNSLSNQWLYAGGLGLDLVTYYDMVIGIDYSV
                     NKQCNHGFFSWKNFFFSIKVPFI"
     gene            complement(7500..8561)
                     /gene="mutY"
                     /locus_tag="PLM68_05230"
     CDS             complement(7500..8561)
                     /gene="mutY"
                     /locus_tag="PLM68_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT54801.1"
                     /translation="MDFTTLLLAWYHENKRDLPWKKTQDPYPVWVSEVILQQTRVGQG
                     TDYIRKFLETFPTVRHLAAAQEDEVLKLWQGLGYYSRARNMHKAAKKLMEEYNGKFPA
                     DVTLLRNLQGIGEYTAAAIASFAYGLPVAALDGNGYRILARFFGIDLEVGTTRAKKVF
                     SNLAGSLVPTHDSASFNQAMMDFGSLVCTPRPVCALCPMTKQCIAWNKGLTDILPVKK
                     GKKLSRNRHFNYLHIVFGETTFIRQRQGNDIWKGLYEFPLIETTDETGFTELARTEPF
                     IRLLGSSSFTLTDTLKLSQHKLTHQTIHPVFFRVTIQSAGPVLNNDYIQIDRRDIHHF
                     AISRLTEKYLDFFHNFAAL"
     gene            8695..8979
                     /locus_tag="PLM68_05235"
     CDS             8695..8979
                     /locus_tag="PLM68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT54802.1"
                     /translation="MTKADIVNDIAKSTGLEKIVVQKVVESFMDNVKSSLIQNNNVYL
                     RGFGSFTLKKRAKKTARNISKNTTLVIPEHYIPAFKPAKIFMDKVKNSVK"
BASE COUNT         2427 a         2126 c         2159 g         2573 t
ORIGIN      
        1 tgtctgccgt atgtggaaaa ttaccagtga gcgtatatag cggatctccc caagaaacaa
       61 ggttaccggt atcctgcctt tagaggagtc attcacaaaa aaggcggcac gtgagccggc
      121 ttcaagcctt atctgcacag tcgttttgga atgccacagg attcttacgg attcaatcgc
      181 ataggaattc atgtaaggat ttttcatgat cataggaacg tcatgcagac tgtgagcttc
      241 gtcaaaagga cgatttctca gaaacgggat cctgttcaga tcaggaggga agagagcgat
      301 ctcctggcag gaacccgatc tggatgttcg gggaacatat tgtgtcaaat cataagccgg
      361 agattttttc atgaacctgt ttccgctttg gtactgtgag attgcttcat aaaacgcaga
      421 gatcctggtg gcaacgtcct ccatattatc caggccaaag gccaatgcaa tccttttttt
      481 ggaattgaag aggttggtaa caacagggaa gctggttcca ttgttgtcaa acagcagggc
      541 tttgccacca tggggcaggc tggactgcct gtctgtaatt tctgtgatct ccaggtttgg
      601 atctactaaa cggttaatgg ttatcagctc tttttcctgt atgagggttt tcaggtattt
      661 gtccaggtaa tgaatcataa aaaactcatg gtttaagttg attgattatc atgctaaggg
      721 cagcggttga aaggttgtca gccacaatac ctttttcttc atccaggagc aataccaggg
      781 gtggatcagc cacgttgtaa ttcttgtaaa tacgatcgtc atcaaaggga ttccagacat
      841 tgatccagcc cgtatagcct ccttctataa gccattttct ccacgaaaga taatcattgc
      901 caatataaac ggcaaaaaat accattcctt tgtccgcagc catacgcgag agttcatata
      961 atcgggcggt aacaaggcca cagacagaac atgcgggatc gtaaaaataa agcaccgtat
     1021 aggaagcctc gatttcatac agggtgcagg gatctccgtt ttctgtttcc agtatcagat
     1081 cgggtgcttt gcgaaggacc ggttcctgct tcaggttttg gtttgtcaac agctcttcca
     1141 ggttttcagt attcaggtcc ctgccaatga ttatcttgtc cttgtcaaga agaaagatct
     1201 ttggagtgct cctgacattg aatttttcct ggaaaccact actcatatcc gggtcccata
     1261 cggaaatcca gtcttcaggg aaaaaatctg catacctgga cagaatatct ttattgtcct
     1321 gcgtgtagac agcatagtag tgtatgtcat cttctgtgtg atcctgtgca aaccgtgtca
     1381 gtttcagtaa ttcactactg catgcagggc aattgtcctc aaagaatacg acaatggtat
     1441 acctcgcatc cagatcatgc agtgactgct cccgggaatg tacatcgggc agtttcaggt
     1501 caggcgcctt cattccgagc aggcactgtt tgttgaaacg tacaaaaatt tctatttcac
     1561 gtacaagatc agggtccgga cacagtgatg tatctgccag cagccatttt tcagccatat
     1621 gaacggctgc actttccact cccattattt cactggtgaa gaacagggtg aacaggtacc
     1681 tggcaacata cccctttacc ttgggatgta tgtttccctt caggatccgg tctgcatgaa
     1741 aaaccaggga atcgggcagg ggaggcagta tggatgtaaa atattcctta agtcttacag
     1801 gcaggatgga tgtgttaagg atacgcgaat ctgaaaaatc aatgtgatcc aggtaatgaa
     1861 gtggtgtttc agcaggagga acagagggtg taaagatgtt cccggctatg atgccaagca
     1921 tctgcccctc aaattgccgt gcaacgaaag acgcatattc ccttatggtg ctttccaggc
     1981 gttgcacctc agccagagcc tgttcaggat ccctgttccc atcaagcatc tcgttggccc
     2041 gggaataatg ctcctgcagg aaccggttaa accgtatgta agctgcgttc tcttccgatc
     2101 cgtctgccct gacatcaagg gtacgtccgc catccatatg agcagtaaaa gagaatgtta
     2161 tatcttcctg tcctgaaatc aggaaattga acaacctctt gttgttctgt acaaagacat
     2221 actgtccggc ttcaagtggg ctgtttcctg taaatctcac acaacctgca tgatccggat
     2281 taagtgtgtc cacaggtacc acatactctc cctgctgaat ggccagaatg accgattcat
     2341 tgctcccccc gttcaggcac agggaaatgc tataggagag gaagataaag agagaccaca
     2401 tgttcggcct acattgttaa atacttattc aggtcaagtc ccgggggcag catgcacgtt
     2461 gcatcccctc cgttcagtag cacatcccgt accacagttg aagaaatgaa agaatatttt
     2521 ggtcctgagg gaatgaagat ggtctggatc tcaggagcca tttttgaatt gacctgtgca
     2581 ataacctgct ctaattcaaa gtcagtagtg gtgcgtatcc ccctgaaaat aaaattgatt
     2641 ttatttttcc tgcagaaatc aattgtcagg ccgttatagg aacatacttc cacatcggga
     2701 aggtctttga tcacttcccg tataatcgct actctcgcta ccggggaaaa aaggcttacc
     2761 ttctgctggt tcacacccac agccacaact atcttatcaa aaagttttct tgcagagatg
     2821 agcacatcca ggtgtcccga agtgaaggga tcgaaagagc cggggaagac tgcggttttt
     2881 gccatggtcg gtatgctttt gttgaatatc caaaagtaag aataatatcg gactttgcct
     2941 cttttcagct acaaccgcca gtcaacaggc tggatgcatt ttgatgcaag gtacatgttg
     3001 gtggcagaga agggctttga tccgaggaaa gcacctcgtg ccagtgggga ggggtgcgct
     3061 gcctccagta tacagtgcct ggacgtatct ataagccctc tttttgaacg cgcgaaattt
     3121 ccccacaata aaaagacaac atggtctttt tgatcagaaa ccgtccttat aacggcatct
     3181 gtgaaatcca tccagcccgt tttactatgg gatgccggtt ttcctgcttc cacagtgagt
     3241 atggcattaa ggagcaaaac accctgccgc gcccagcctt ccaggcaacc gtgacggggt
     3301 gtggagatcc cgaggtctga cctcatttcg gtgaatatgg tctgcagcga tggaggttgc
     3361 ggcatggtga caggtacaga aaaggacaag ccgtgcgcct gccccggctg gtggtaggga
     3421 tcctgcccta gcaggaccac tttcagggca tgaaaaggag tcagtgcaaa agcgttgaat
     3481 atgagtttgc ctgggggata tatggttttt ccctgcctga tctcatcatg gagaaaagag
     3541 gcaatcccct caaaataggg tttggaaaac tctggctgca atacattttt ccagcttgcc
     3601 tcaattttca catccacctt acaaaacgta tttgattact tcgggtatgg ttttcatgaa
     3661 tttgaactcc agacctttaa tgaaatcagg cttgatttcc tcgatatcct tgcggttgtc
     3721 ctcagacagg ataatggtct tgataccgga acgcttggct gcgaggattt tctctttaat
     3781 gccccctacc ggcagtatcc tgccacgcag ggtgatttcc ccggtcatcg ccacgccttt
     3841 tttgatgcat ttgttgagga aggaactggc catggaacag atcatggtaa tgcctgcaga
     3901 tgggccgtct ttgggaatgg ctccttcggg aacgtgcagg tgaatatccg tactttcaaa
     3961 aatccccggg tccagcccca gcatgtcggc atgtgctttc aggtattgct gtgcgataat
     4021 tgccgattcc ttcatcacat cacccaggct gccggtggtg gtaagtgtcc ctttcccttt
     4081 gctcaggctc gtttctacaa acaagatctc tcctcctgtt tcggtccatg ccagtcctgt
     4141 gaccactccg ggaaactcat tgcctttctg cacatcctgt atgatcctgt gaacacccag
     4201 tatctcacga atattttccg gtgtaagaac agggtcgtat tcttccccga aagcgatttt
     4261 tttggccaca tgcctggcca ccttggccag ctgtttatcc aggttacgca ctccggattc
     4321 ccttgtatat tcattgatta tgacatctat ggactgacgg ccgagtttga tttgcgatgg
     4381 tttcaaccca tgggcttcca gttgtttggg caaaaggtgt ttcaacgcga tggtgcgttt
     4441 ttcctcggcc aggtatccat tgataggtat catttccatg cggtcacgca atgccggttg
     4501 gatacctccg atattgttgg cggtggtaat gaaaagcacc cttgaaagat cgtagtccac
     4561 atccaggtaa ttgtcgtgaa aggtgttgtt ctgttcgggg tccagcactt cgagcaaagc
     4621 cgaggagggg tctcccctga aatcggtacc cactttgtca atctcgtcca gcacaactac
     4681 cggattggaa gttttgctcc gcttgatgct ctgtatgatc cttcccggca tcgccccgat
     4741 gtaggtacgc ctgtgtcccc tgatctctga ctcgtcatga aggccaccca gggaaatgcg
     4801 ggcaaagttc cttccaagtg cccgggcaac cgatttcccc agtgaagttt taccaacgcc
     4861 cggagggccg taaaggcaaa gtatgggaga tttcatgtct cctttgagtt tcagcacagc
     4921 caggtgttcc aggatccttt ccttgaccgt ttcaagtcct gaatggtcct tgtcaagcac
     4981 cctgcgggcc cgcttcagat ccaggttgtc tttcgtatat tctccccagg gcaactcaac
     5041 catgaattgc aggtaattga gctggattgc ataatcgggc tgatgcggat tggccctttc
     5101 cagtttctgg acttcctttt cgaatatttc tgcaatttct ttgctccatt tcttattagc
     5161 cgctttttcc cggagggtat cgacttcttc catgtcctgc gtgaggccca gctcatcttg
     5221 tatggtttta agctggttac tcaggtaata ctctttctgt tgctgatcaa tctcaccacg
     5281 cactttctgt tggatctgct ctttgaactt aagcaattca agatgcttgt atagcaattc
     5341 aagcagacgg gaagcacggt cattcagttt gtcaatagac agaagcgcaa gtttatcttt
     5401 gattccgtcc aggtatatgt tggaagctac aaagttgaca aggaaatcat ccttttcaat
     5461 attgcggagt gcaaattctg cttctctagg tatgcttggc gtaagctgaa aaatctgggc
     5521 cgcaacatct ttgatgccat cgaccaccgc tttgtaacca cgatcatctt gagcaggatg
     5581 gatgtcgttc aaatagatta cctgggcggc aaaataaggc tctttctgca cgatacggtc
     5641 aatcttgaaa cggcggaaac catgaaggat aacagtaatc ccaccttctg gcatttcaat
     5701 gatcttcaat atttttgcca gacaaccgtg gtcgctgatc tcattttctt ccggatcgtc
     5761 atcaagagga tccatttgtg tgaccgtgcc aacccattta tctccggtca gactttccct
     5821 taccagttta atagattttt ccctgccaac cgtaatgggg atcagggtgc cgggaaatac
     5881 aaccgcgttg cgaagcacca gcagggggag cacctgcggc atcccgtctt tgatcatatc
     5941 tcctgaagtc tggtccatgt ccatttccat caaggggatg acggtcattt tatcacttaa
     6001 tgttaaattc atatcattac tactagcaaa caatcagtca atcttcgtgc cagatggcga
     6061 tactgacaaa atggccgatg atcaaatgaa cggaactttg atggaaaaga agaaattctt
     6121 ccaggaaaag aaaccatggt tgcactgctt gttgaccgaa taatcaatac ctatgaccat
     6181 atcatagtat gtaaccaggt ccaggccaag acccccggca tatagccact gattactcag
     6241 ggaattcagg tgataatccc atttattcct tgcatagccc gcatcaaaat gagcgttggc
     6301 atagatggac aggtggatct tgttgaattt gggaaggaat gagagccagt taagcgtgaa
     6361 agtccttttt ttaagaatgt tgaactttaa ggtattgttg aagatgacaa aggactgccc
     6421 gtcagccact atgtattcgt aaccgcgcaa catgttgttg tcatatccta tagccctttc
     6481 cagtatataa gcctgagcgt tggacaggga ataacctccg gaaaaaacgg atgagacata
     6541 ccagcgtgat gcaccgagtt gtttgaaata ctgtagcccg agtgtgacct gggcatagcg
     6601 cacggaagcg tcagtagtaa tataggtgtt cagccctgcc tgcaggaaat aaccattcag
     6661 agggtatggg tggtaatcgc ggtgatccag gcggtacctg taattcagcc ggaaagcgtt
     6721 ccttttgagc tggtcattac cccagtagtg cctgttcagg cttagcacgg tatctgccag
     6781 cagggtgtat tcatgcatga gggatacgtt gtggattgag cggatgtccg gccggtacgt
     6841 ataattgata cgggtatcga tggttttttc aagatatatg tcccggatgt taaagcgggc
     6901 cggcttgtca ttctgtgtga aaatgtccag gttgtggctt atgttcccct gcaggtccac
     6961 gctcatgaaa tgtttgccct gtctgtccag ggaaatgtcc cggtatccca ggaaaacacc
     7021 ccattgataa cctccgtgcg ctgctatgac cagcttatgt ttcaagccca ggaagttgtc
     7081 tatccgcgct cctgcttcaa tggtgatctt ggtgaaatcc cagctttgca gccatgtact
     7141 cagattgcgc tcctcaagtt ttacttcaat aagaggccag atataccatc tttcttctac
     7201 ctgaataatc actgtcacaa gggaagagtc atctgccgag cggagaggtg atatctcgat
     7261 aaaattgaat agcagaaggt tgttcaggtt ttcccttgcc aggcgcaatt tgtcaggaaa
     7321 tacttcttcc gggatcgtgt caccctttga aaaagggagc tccctgagga tgataaaatc
     7381 cttggtcacc ttgttaccgg taagcaggat cccgtcaata cgcaaattcc cgctccatcc
     7441 tgcgtgtgac aacaataaca gacagacagt aaataacagg cgaaggagcg ggttagattt
     7501 catagggcgg caaaattatg aaaaaaatcc agatattttt ctgtcagcct ggatattgca
     7561 aaatggtgta tatcgcgccg gtcaatttga atatagtcat tgttcaacac cggaccggca
     7621 gattggatcg tgaccctgaa aaaaacagga tgaattgtct ggtgtgttag tttgtgctga
     7681 ctgagcttaa gtgtatcagt caacgtaaag gatgaggacc ctaacagccg gatgaaaggc
     7741 tccgtcctgg caagttctgt gaatccggtc tcatctgtgg tttctatcag gggaaattca
     7801 tagagacctt tccagatgtc attgccttgc ctttgcctta tgaaggtggt ttccccaaaa
     7861 actatatgca ggtaattgaa gtgtctgttc ctggataatt tttttccctt ttttaccggt
     7921 aaaatgtctg tcagcccctt gttccaggca atgcattgtt ttgtcatggg acacaatgca
     7981 caaacaggac ggggggtgca gacaagcgat ccaaaatcca tcatggcctg attgaaagag
     8041 gcgctgtcgt gggtgggaac taaagagcct gccagattgg aaaacacctt ctttgcccgg
     8101 gttgtgccca cttccaggtc tatgccaaaa aacctggcca ggatacgata tccgttcccg
     8161 tcaagggctg cgacaggcag tccgtaagca aaagaggcaa tggcagctgc tgtgtactca
     8221 ccaatgcctt gaagatttct caaaagggtg acatcggccg gaaacttccc gttgtattct
     8281 tccatcaatt ttttagcagc tttatgcata tttcgtgccc gggaataata acccagtccc
     8341 tgccacaatt ttagcacttc atcttcttgt gctgcagcaa gatgacggac agtgggaaaa
     8401 gtttccagaa actttctgat ataatctgtt ccctgtccga ccctggtttg ctgcagtatc
     8461 acttcagaca cccatacagg ataagggtct tgtgtttttt tccagggcag gtcacgctta
     8521 ttctcatgat accaggccag taggagggta gtgaaatcca tggcacaaag atatgcataa
     8581 tgatattttt attgtttttt taacttaaac gtcttgatat tacaaattaa atgttacttt
     8641 tgcaatccac ttagaatgca atctaaataa ataacttttt aaaatcttcg tattatgact
     8701 aaagcagata tcgtaaatga cattgccaaa tctacaggcc tggagaaaat cgttgtacag
     8761 aaagttgtgg aaagcttcat ggataacgta aaaagctctt tgattcagaa taataatgta
     8821 tacttgcgcg gtttcggaag ctttaccctg aaaaaacgtg ctaaaaaaac agctcgcaat
     8881 atatccaaaa acacaacatt agtaattcct gaacattaca tacctgcttt caagccggct
     8941 aagattttca tggataaagt aaaaaacagc gttaaataat tattatatgc caagcggaaa
     9001 aaaaagaaag aggcataaaa tggctacgca caaacggaaa aaacgtttgc gcaagaatcg
     9061 tcataaaaag aaataagcca taatgtctga tgaacctgga aacctaaagc aacttttttc
     9121 taaaagacac tttaggtttc ttacttttgc cacagaatgg agaaagattt aattattgat
     9181 gtaacctctt ctgaagtctc catagcattg ctggagaata agaggctcgt cgaattacac
     9241 agggaggaaa caagcgacaa tcatttctcg gtgtgcgatt tgtac
//
LOCUS       DAOSOF010000071         9266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7395_length_9266_cov_5.375964, whole genome shotgun sequence.
ACCESSION   DAOSOF010000071 DAOSOF010000000
VERSION     DAOSOF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7395_length_9266_cov_5.375964"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLM68_05240"
     CDS             <1..1011
                     /locus_tag="PLM68_05240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT54803.1"
                     /translation="CNGRLPCGNPGRTPIVSEMNRYQFNKEKLEFTRVRYNVKTILKR
                     IFWFFLASIGLALIYYLIFGLFILSPAERELIRETQIMQSEYKRLSGEMGPLNKVLEE
                     LEVRDKSIYGTVLHSRPLDVTEDADISHYRQLMEDPSFALAYHADSVITGLELRALWV
                     TGILYQVTNSIYDKLDSLKYLPAAFPLRTPSMEAMGATTGRRIHPFYKIPMDHTGMDF
                     LVGLGTDVLATADGQVVNVARSARGKGNVVEIRHPYRGYSTVYAHLSDIFVREGQYVT
                     RGMTIARVGSTGMSFMPHLHYEVWQNDLVMDPVHYYFMDVMPREYRQIMLTAYNTGQS
                     LD"
     gene            1017..1337
                     /locus_tag="PLM68_05245"
     CDS             1017..1337
                     /locus_tag="PLM68_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT54804.1"
                     /translation="MEKMYYSIGEVAQILGESTSLVRFWSTTFSDFIKPVRNKKGNRL
                     FTAEDLATYKTIHHLVKERGLTLEGTKKRLKDNKEGEDRTVDIIASLNSIKSQLAEIK
                     DMMG"
     gene            1334..2134
                     /locus_tag="PLM68_05250"
     CDS             1334..2134
                     /locus_tag="PLM68_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT54805.1"
                     /translation="MKTTVADIIEVLEHWAPQGLQEEWDNSGLCIGNSTTIVRGVLVC
                     LDCTPEVLEEALADGLNMVISHHPLIFKGLKSLKDSTPVERTVAAAVRGNMVVYSMHT
                     NADKIMDGVSGAMARALKLKNLSILAPESCQEQDAAPCGLGVVGDLEYPMATKDFFAM
                     LKSTFALKHCKASESFHSQVKRVALCGGNGSSLIPYALRSGADIFVSADFSYHTYFET
                     VPQLMLADIGHYESEAGVQDQIVNQLMKKFPNFAVRKTQVYTNPIRYY"
     gene            2236..2952
                     /locus_tag="PLM68_05255"
     CDS             2236..2952
                     /locus_tag="PLM68_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT54806.1"
                     /translation="MEEKLRLLFQLQTTDSQIDKIHLLRGELPLEVQDLEDDIKGLAT
                     RMENLLKEIRDIEKDIAFRRIDADNARGMVARYEAQQANVKNSREFDSLAKEIENQVL
                     DAEMAEKYIREFSILLKDRKETLEKTKNDHLERQKDLEAKKKELESIISETAKEEEAL
                     LRKVEHLESQIDARVLSAYRKVRKNAHNGLAVVTVKRDACGGCFNKIPPQRQLDIKMS
                     KKIIVCEYCGRILVDPAFEE"
     gene            2960..4141
                     /locus_tag="PLM68_05260"
     CDS             2960..4141
                     /locus_tag="PLM68_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT54807.1"
                     /translation="MREQFYALLGQTSPSPLGIEIQKAEGVFLLSTSGKRYLDLISGV
                     SVSNVGHARREVIQAIADQAANHLHLMVYGELIQRPQVLLAALLTENLPATLNAVYFV
                     NSGSEANEAALKLAKRITGRHKIACFRNAYHGSTHGALSVMGNETYRRSFRPLLPGVT
                     ELRFNHLPDLGLIDKGTACVIAEPVQAEAGVRLPEQGFLQSLRQRCNDTGTLLIFDEV
                     QTGFGRTGSLFAMQKYGVTPDMVTLAKALGGGMPLGALVCGKELMEAWKSEPVLGHIT
                     TFGGHPVSCAAGFAALQVLLREGWTQQVQEKASFLRKALAGHPAITEIRGDGLLLAVE
                     LGCARKVQDMIRLLLEEGAIADWFLYCDTAFRIAPPLCINREELYLAAGIISRALDKL
                     K"
     gene            4156..5658
                     /gene="dnaB"
                     /locus_tag="PLM68_05265"
     CDS             4156..5658
                     /gene="dnaB"
                     /locus_tag="PLM68_05265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT54808.1"
                     /translation="MAKRAPDTSTAKGKGQAQVETVGLDMGKVPPQAIDMEEAVLGAL
                     LLEPNASLDIVGVVKPEYFYKESHQKIFSAITDLVTEHNPVDIYTVAEELKKHNQLDE
                     VGGPYYLSQLTMRVAAASHLEYHSKVLTQKFIQRELITTSYGILRDAFDDTIPADELL
                     DASQQKLFQLGEMNLRRDTRPVRSVLGEVLDELSDVQTRDDGLSGLPSGFTSLDRMTL
                     GWQKADLIVIAARPSMGKTAFVLSMARNMTVNHNIPVAFFSLEMPDAQLVKRLLVSET
                     GLSSEKIRGGKKLKSFEWEQLNQRIINLTKAPLYIDDTPSLSIFEFRAKARRLVAHKG
                     IKLIIIDYMQLMSGPPELRGMREQEVASISRSLKAIAKELNVPVVALSQMNRSSETRG
                     GNRRPQLSDLRESGAIEQDADIVIFLYRPEYYGFKEDDAGRSLEGIAEVIMAKHRNGA
                     VGEFQMRFRSSEIRFMDINEGGPDFAPGESITYASRMNQMNDLNDNFGGF"
     gene            5636..6490
                     /locus_tag="PLM68_05270"
     CDS             5636..6490
                     /locus_tag="PLM68_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOT54809.1"
                     /translation="MTTLAAFRFFILTSFLLTVSWLSPAQEVEKPCLSVRDIPESQLA
                     FGDGEKLTVIASYTWGVLNTDVGTVTLEISEKNEERAPQFVAKGRIQTARFFNAFFRV
                     DDYYESHFKKSNLRPTYFMRDIKEGKYTIQNYYHYNADHSINARIVRKDGSVQDTLLP
                     GRVCTFDFITIFYFLRNLEFKNMKPGDVSPISFAIDEEIFDLYLRFDGRDEIRIQGLG
                     TFRCLKFSAQTVAGVVFDGKEELRFWISDDRNRIPLLIETPVVMGRVTGRLGSYENLR
                     FPLTSKIK"
     gene            6881..7726
                     /locus_tag="PLM68_05275"
     CDS             6881..7726
                     /locus_tag="PLM68_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOT54810.1"
                     /translation="MTSTLLYTVLSLSILGGLLAVILFFVAQKFKVQEDPRIDQVVEA
                     LPGANCGGCGFAGCRAFAEFYVNNPEATDYLCPVGGNEVMLRVAQMLGRDAREQAPKV
                     AVLRCNGSCEILPRLNKYDGSPSCTVAADLYGGQTGCSYGCLGMGDCVKACQFDALVM
                     DPDTCLPVVLEDKCTSCGACVKACPRNIIELRPKGPKNRRIYVTCANKDKGAVTRKVC
                     SVGCIGCQLCMKACPFEAITITDNLAYIDPAKCRLCRKCAPVCPAGCIAEINFPPKKQ
                     ETGNE"
     gene            7719..9065
                     /gene="rsxC"
                     /locus_tag="PLM68_05280"
     CDS             7719..9065
                     /gene="rsxC"
                     /locus_tag="PLM68_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT54811.1"
                     /translation="MSKTFSIGGIHPDDCKFSRDCPIEDFPIPKRVYISMNQHLGMPA
                     VPVVKKGDPVRVGQLIAEPAGFVSAPVHASVSGTVSDVTPYPDFLGKKVLTVVIDTDG
                     DQWEEGIDRSPEIRHEITSSPEDVFKRISESGIVGLGGAAFPTHVKLTPPEGMKPEYL
                     ILNGAECEPYLTADYRLMLEHGQEILLGGELMRRTLGAGVNGIIGIEANKPRAIELMR
                     ELTSKSLQWKVVPLKKRYPQGGEKQLIDAVLGRRVASFALPVSTGAIVQNVSTAYAVY
                     EAVQKNKPLISKVLTVSSSDSSIQKNYRFRVGTPIMDILSHAGAMEKDGGLSPQFAKV
                     VNGGPMMGKPVSNLEGPVMKSTSGLLLFTEKETKRKEPGECIRCAKCFEACPMRLQPY
                     LLYQLALAGRTEELEEHRVFDCIECGCCLYTCPAHLPLLDQIRLARNASLKNKRKK"
     gene            9067..>9266
                     /locus_tag="PLM68_05285"
     CDS             9067..>9266
                     /locus_tag="PLM68_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT54812.1"
                     /translation="MDKLLVSPAPHLHSKENTRRIMRDVIIALMPSVLVSVYFAGLSA
                     LLVVVVSVAGCVLVEHLIVRYL"
BASE COUNT         2443 a         2187 c         2453 g         2183 t
ORIGIN      
        1 tgcaacggac gattaccatg cggtaatcct gggcggacgc caatagtttc tgaaatgaac
       61 cggtatcagt tcaataagga aaaattggaa ttcaccaggg tgcgttacaa tgttaaaacc
      121 atcctaaagc gcattttctg gttttttctt gccagcatcg gacttgcact gatctattat
      181 ctgatcttcg ggttgtttat tctgtcgcct gccgaaagag aactcatccg tgaaacacag
      241 atcatgcaaa gcgaatataa gaggcttagc ggtgaaatgg gcccccttaa caaagtgctg
      301 gaagaactgg aggtcaggga taagagcatt tacgggaccg tactgcattc aagaccgctg
      361 gatgtgacag aagatgccga tatttcacat taccgccagc tgatggaaga cccttccttt
      421 gcgctggcct accatgcaga ttctgtgatc accggcctgg aactacgggc attgtgggta
      481 accggcattc tttaccaggt aacaaacagt atatatgata aactggacag cctgaaatac
      541 ctcccggctg cctttccgct tcgaacccca tccatggaag ccatgggagc aacgacgggg
      601 cgccgcattc atccttttta taaaataccc atggatcata caggcatgga tttcctggtg
      661 ggactgggga cagatgtgct ggcaacggcc gatggccagg ttgtcaatgt ggcacgttct
      721 gcaagaggca agggaaatgt tgtggaaatc aggcatcctt acaggggtta ttccaccgtt
      781 tatgcacatc tgagtgatat ttttgtcagg gaaggtcaat atgtaaccag gggaatgaca
      841 atagcacggg tagggtctac gggcatgtcc ttcatgcctc atctccatta tgaagtgtgg
      901 cagaacgacc tggtcatgga cccggtccac tattacttca tggatgttat gccacgtgag
      961 taccggcaga tcatgcttac agcttacaat acaggacaat ctttagatta aggaatatgg
     1021 aaaaaatgta ttactctata ggagaagtgg cgcaaatact gggagaaagc acttccctgg
     1081 tacgtttctg gtccaccaca ttcagcgatt ttattaaacc cgtacgcaat aaaaaaggga
     1141 acaggctatt cacggcagaa gacctggcta cgtataaaac catccaccac ctggtgaaag
     1201 aacgcgggct taccctggaa ggcacgaaaa aacgcctgaa ggacaacaaa gaaggcgagg
     1261 acaggacagt ggacatcatt gcttcactca acagcatcaa gtcacaattg gcagaaatca
     1321 aggacatgat gggatgaaaa ccacggtagc tgacatcata gaagtacttg aacattgggc
     1381 cccgcaggga ttacaggaag aatgggataa ttccgggctt tgcataggca attccaccac
     1441 aatagtcagg ggagtcctgg tatgccttga ttgcaccccg gaagtgttgg aagaagccct
     1501 ggcagatggt ctgaacatgg tcatatcgca tcatccgctc atattcaaag gcttgaaaag
     1561 cctgaaggac agtactcccg tggaacgtac cgttgccgct gcagtccggg gcaacatggt
     1621 ggtttattcc atgcatacca atgccgacaa gatcatggac ggtgtcagcg gggctatggc
     1681 ccgggctctg aaactaaaga acctttccat tctggccccg gaatcatgcc aggaacagga
     1741 tgccgcccca tgcggtcttg gcgtggtggg ggatctcgag taccctatgg caaccaaaga
     1801 tttttttgcg atgctcaaat ccacctttgc cctgaaacat tgtaaggcct ccgaatcctt
     1861 tcacagtcaa gtgaaaaggg tggccctttg tggtggaaac gggtcttccc tgattcctta
     1921 tgcattacgt tccggtgcag atatctttgt aagtgcagat ttcagttatc acacctattt
     1981 cgagacagtt ccgcaattaa tgctcgcaga catagggcat tatgaatcag aagccggggt
     2041 gcaggaccag atagtcaacc agcttatgaa aaaattccct aactttgccg tccgcaaaac
     2101 acaggtctac acaaatccca tcaggtatta ttaattatca attagattat gacaaagaaa
     2161 acgacaaaaa ctgcaggaac agatgccgga tcatttacag ccctgcaaaa agcagaacag
     2221 gaaggtacgc tgaccatgga ggaaaaattg cgtcttcttt ttcaacttca gacaacagac
     2281 tcacagatag acaaaataca cctgttacgt ggcgaactcc ccctggaagt ccaggacctg
     2341 gaggatgaca ttaaaggcct tgccaccaga atggagaatc tgctcaagga gatccgtgat
     2401 attgaaaaag acattgcatt tcgcaggatc gatgctgaca atgcccgtgg catggttgcc
     2461 aggtatgagg cccagcaggc taatgtaaag aatagccgcg agtttgattc cctggccaag
     2521 gaaattgaaa accaggtatt ggatgccgag atggcagaaa agtatatccg ggagttttca
     2581 atcctgctca aggacaggaa ggagacgctg gaaaaaacaa agaacgacca cctggaaagg
     2641 cagaaagacc tggaggccaa gaaaaaagaa ctggaaagca ttatcagtga aacggccaag
     2701 gaagaagaag ccctgctccg caaggtggaa catttagaaa gccagataga tgcgcgcgtc
     2761 ctctctgcat accgcaaggt ccggaaaaat gcacacaacg gtcttgccgt tgtgaccgtt
     2821 aagcgcgatg cctgcggcgg ctgtttcaat aaaattccgc ctcagcgcca gcttgacata
     2881 aagatgagca aaaagatcat cgtatgcgaa tactgcggaa gaattttggt tgatcctgct
     2941 tttgaagaat aacccttcca tacgagaaca attttacgcc cttctaggcc aaacctcccc
     3001 ctctccctta ggtatagaaa ttcaaaaagc agagggggtg tttcttctgt caacttcggg
     3061 taaacgctac ttggatctca tttccggagt gtctgtaagc aatgtgggcc atgcccgcag
     3121 agaagtcatc caggctatag cggaccaggc tgccaaccac ctccacctga tggtatacgg
     3181 tgagcttatt caacggcctc aggtccttct tgccgccctc ctgacagaaa accttcccgc
     3241 aacgctgaac gcggtttact tcgttaattc cgggtccgag gcaaacgagg cagccctgaa
     3301 gctggccaag cgcatcaccg gaaggcataa gatcgcctgt ttccgcaatg cataccatgg
     3361 aagcacacat ggtgccctga gcgttatggg aaatgaaaca taccggcgtt ctttcaggcc
     3421 tttgttgccc ggtgtaacag aattgcggtt caaccacctc ccggacctgg gtttgattga
     3481 caagggtact gcctgtgtca tcgcggaacc ggttcaggca gaagccggcg tccggcttcc
     3541 tgagcagggt ttcctgcaat ccctccgcca gcggtgtaat gatacgggaa ctttgcttat
     3601 ctttgatgag gtacagacag gtttcgggag gaccggcagt ctctttgcca tgcaaaagta
     3661 tggcgtgacg ccagatatgg ttacattggc caaagccctg ggcgggggaa tgcctctggg
     3721 agcattggta tgtggcaagg agctgatgga agcctggaaa tccgaacctg tcctgggaca
     3781 tattactact tttggaggac accctgtcag ttgtgctgcc ggttttgcag ccttgcaggt
     3841 gttgttgcgc gaaggatgga cacagcaggt tcaggaaaag gcatcttttc tcaggaaggc
     3901 ccttgccggc catccggcca taacggagat aaggggggat ggattgctgc tggccgtgga
     3961 actgggatgt gcgcggaaag tacaggatat gatccgcctg ttgctggaag aaggcgcaat
     4021 cgcagattgg tttttatact gtgatacagc atttcgcata gctccacccc tatgtatcaa
     4081 ccgggaagaa ttgtacctgg cagcaggaat catcagcaga gctttggata aattgaaata
     4141 aaaaggagat gaattatggc aaaacgagca cctgatacaa gtactgcgaa aggaaagggc
     4201 caagcccagg tggagacggt agggctggat atgggaaagg ttccgcccca ggccattgat
     4261 atggaagaag ccgttcttgg cgcattgttg ctggagccga atgcgtcgct cgatattgtg
     4321 ggggtggtca agccggaata tttctataag gaaagccacc agaagatctt ctcggccata
     4381 acggacctgg ttaccgaaca taaccctgta gatatctata cggtagcgga agaattgaaa
     4441 aaacataacc agcttgatga agtaggaggt ccctattacc tgagccagct tacaatgagg
     4501 gttgctgccg cctcgcacct ggagtatcac tccaaggttt tgacacaaaa attcatccag
     4561 cgtgaattga tcaccacgtc ttacggcatc ctgcgcgatg ctttcgacga caccataccg
     4621 gccgacgaat tattggatgc ctcgcagcaa aaactctttc agctggggga aatgaacctt
     4681 cgccgggaca cgcgtcccgt gcgctccgtc ctgggggaag tgctggacga actgtccgac
     4741 gtacaaacca gggatgacgg attgagcgga cttccctccg gttttacttc ccttgacagg
     4801 atgaccctgg ggtggcaaaa ggccgatctg atagtcattg cagcaaggcc ttccatggga
     4861 aaaacggctt ttgtcctgag catggcaagg aacatgacgg tgaaccataa tatcccggtg
     4921 gccttctttt ccctggaaat gcccgatgca cagctggtaa agcgattgct ggtgagcgaa
     4981 acgggtttgt cttccgaaaa gatcaggggt ggcaagaagc ttaaaagctt tgaatgggaa
     5041 cagctcaacc agcgtattat aaacctgacc aaagccccgc tgtacataga cgataccccg
     5101 tcactttcca tattcgagtt ccgggctaag gcaaggcgcc tggtggcgca caaggggatc
     5161 aagctcatca taatcgacta tatgcagctg atgtcaggtc cacccgaatt acgggggatg
     5221 cgtgaacagg aagttgcctc catatcacgc tcgctgaaag ccattgccaa ggaactgaac
     5281 gtgcctgtgg tagccctttc ccagatgaac cgttcttcag aaacaagggg tggcaaccgc
     5341 cggccgcagc taagcgacct gagagagtcc ggcgctattg aacaggacgc cgatattgtc
     5401 atattccttt accgtcctga atattacggg ttcaaggaag atgatgccgg gcgttccctg
     5461 gaagggatag ccgaggtgat tatggctaaa caccggaacg gggccgtggg agaatttcag
     5521 atgcgtttcc gcagcagcga gatacgtttc atggatatca acgaaggtgg tccggatttt
     5581 gcacccggag agagcattac ttatgcctcg cggatgaatc aaatgaatga tctgaatgac
     5641 aactttggcg gcttttaggt ttttcatcct tacatctttt ttgctcacag tatcgtggct
     5701 ttctcccgcg caggaagttg aaaaaccatg cctttcagtc agagatatac cggaatccca
     5761 gctggccttt ggtgacggag agaaactgac cgtcattgcc agttatacct ggggtgtgct
     5821 taatacagac gtaggtactg tcacgcttga gatttctgaa aaaaacgaag agagggcacc
     5881 gcaattcgtt gccaagggcc gcatccagac agcacgtttt ttcaatgcct ttttccgggt
     5941 ggatgattat tatgaaagcc atttcaaaaa gtcaaacttg cgtcccacgt atttcatgcg
     6001 tgatataaaa gaaggcaagt acaccatcca gaattattac cattacaatg ctgatcattc
     6061 catcaatgcc aggattgtcc gcaaggatgg ctctgtgcag gataccttgt tgcctggaag
     6121 ggtctgtact tttgatttta tcaccatctt ctattttctg agaaacctgg aattcaaaaa
     6181 tatgaaaccg ggtgatgtgt cacccatatc ctttgccatt gacgaggaaa tatttgacct
     6241 ttacctcagg ttcgacggca gggatgaaat ccgcatacag ggactcggga cattccgctg
     6301 cctgaaattc agtgcacaga cggtggcagg agtggtgttt gacggcaagg aagaattgcg
     6361 gttctggatc agtgacgatc gtaacaggat acctttatta attgaaaccc ccgttgtaat
     6421 ggggcgggta accggcaggc tgggcagtta tgaaaacctg cggttcccat tgaccagtaa
     6481 aataaaatag caatgcctcc cgttcagtgc gtcagacaca ggggaatcat cacagggatt
     6541 aacccgctca gggcagaaat caccaccagc ggagcatgtg aggcctgtca tgccaaagga
     6601 tattgttcac taggccagac cagtataaga gagataacac ggttgaaaga acctgaggga
     6661 tgggtgccag tcttgggaca ggaggtgttt attgagatgc atacccggat gggattcaaa
     6721 gccctgtggt acgccatggg tttgccatca atcattttgg gagtcttggt tgctgtttcc
     6781 gcgctacttc atttgcccga ttggctgttg ggatcagtag cattatctgg tgttggaatc
     6841 tattattttg ttctatattt gttaaaagat cgcatccagc atgacttcta ctttactgta
     6901 caccgtattg agccttagca tattgggggg gctcctggct gtcatattgt tttttgtggc
     6961 gcaaaaattc aaggtacagg aagatccccg tattgaccag gtcgttgagg ccctgccagg
     7021 ggccaattgc ggcggatgcg gctttgccgg ttgccgggcc ttcgccgagt tttacgtaaa
     7081 caacccggaa gcgactgatt atctctgtcc ggtgggaggg aatgaagtta tgttgcgtgt
     7141 tgcgcaaatg cttggcaggg acgccaggga gcaggctccc aaggtcgcgg tgctccgctg
     7201 taatggttcc tgtgagatac tgccccggct gaacaaatac gacggtagcc ctagttgtac
     7261 agtagctgcc gacctgtacg gaggccagac gggctgttca tacggatgtc ttggcatggg
     7321 agattgtgtg aaagcatgtc agtttgatgc ccttgtgatg gatccggaca catgcctgcc
     7381 ggtggtgctg gaagacaaat gcacctcctg tggagcctgc gtgaaagcct gcccgcggaa
     7441 cataatcgaa ttgaggccga aaggaccaaa gaatcgcagg atctatgtga catgtgccaa
     7501 caaggacaaa ggggcggtta cccggaaggt ttgctccgtg gggtgcatcg gttgtcaatt
     7561 atgtatgaaa gcctgtccgt tcgaagcaat taccattacc gacaacctgg cgtacattga
     7621 tccggcgaag tgccgcctgt gccggaaatg tgctcctgtt tgccctgccg gatgcattgc
     7681 agagatcaat tttccaccaa aaaaacaaga gaccggaaat gagtaaaaca ttttcaattg
     7741 gaggaataca tcccgatgat tgtaagttct ccagggattg tcccatagaa gatttcccca
     7801 tacccaaacg tgtttatata tccatgaacc agcacctggg aatgcctgca gtcccggttg
     7861 tcaagaaagg cgatccggtc agggtgggac aactgattgc agaacctgcg ggatttgtct
     7921 cggctcctgt tcatgcctct gtgagcggaa ccgtctctga tgtgacgcca tatcccgatt
     7981 tcctggggaa aaaagtattg accgtggtga ttgacacaga cggggatcag tgggaggaag
     8041 gaattgaccg gtcccccgag atccgtcatg aaatcaccag ctctccggag gatgttttca
     8101 aaaggatttc cgaatcaggg atagtcggcc tgggcggcgc tgcctttccc acccatgtga
     8161 aactgactcc gcctgaaggc atgaagcctg aatacctgat acttaacggt gcagaatgcg
     8221 aaccttatct gacagccgat taccgcctga tgttggaaca tggccaggaa atcctcctgg
     8281 gaggtgaatt gatgcgccgt accctgggag ccggtgtgaa cgggatcatt ggcattgagg
     8341 cgaacaaacc ccgggcgata gaactaatgc gggaactgac ttcgaaatcc cttcaatgga
     8401 aggttgtacc cctgaaaaaa aggtatcccc agggaggtga aaagcaattg atcgatgctg
     8461 tcctgggacg tagggttgcc tcgtttgccc tgcccgtttc cacaggggcc atagtacaga
     8521 atgtgagcac agcctacgct gtatatgaag cagtgcaaaa aaacaagccc cttatatcca
     8581 aagtgttgac tgttagcagt tctgatagtt caatccaaaa gaattaccgc ttcagggtag
     8641 gtactcccat tatggatatc ctctcgcatg ccggggctat ggaaaaggac ggtggcctct
     8701 ccccccagtt tgccaaagtg gttaacggag ggcccatgat gggcaaacct gtttccaacc
     8761 tggaaggacc ggtgatgaaa agtacatcgg gactcctgct ctttacagaa aaagagacaa
     8821 aacgcaagga acccggtgaa tgtatccgct gtgccaagtg ttttgaggcc tgccccatgc
     8881 gccttcaacc ctatctttta taccagctgg cgctggccgg gcgtaccgaa gagttggaag
     8941 aacaccgcgt ttttgactgc atagaatgcg gatgttgtct ctacacctgc ccggcacacc
     9001 tgccgctgtt ggatcagata cggcttgcca ggaatgcttc tttgaagaat aaacgcaaga
     9061 aataatatgg acaaattact tgtttcaccg gctccgcact tgcacagcaa agagaataca
     9121 cgcaggatca tgcgcgatgt gatcatcgca ctaatgcctt ctgttcttgt cagcgtttat
     9181 tttgcaggcc tcagtgcttt gctggtggtt gttgtttcgg ttgccggctg tgttctcgtg
     9241 gaacacctga ttgtccggta cctgat
//
LOCUS       DAOSOF010000072         9192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7481_length_9192_cov_4.313670, whole genome shotgun sequence.
ACCESSION   DAOSOF010000072 DAOSOF010000000
VERSION     DAOSOF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7481_length_9192_cov_4.313670"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLM68_05290"
     CDS             <1..667
                     /locus_tag="PLM68_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT54813.1"
                     /translation="AIIITPVGHPMAEKVQMPKPGFLEGVRALADKYGTVLVFDEIRS
                     GFRYDLGGAQKLFGVTPDLSVFGKAMANGYPIGAVVGKEKYMKVLADKVFLSSTFFPN
                     SLEQVAALKTIEILERDNILDKVKAKGELFGKRLQEIVRDVPYDVEVSGIPLMPFITF
                     KKDADNLYKKLRPGFYTELIRRGVFLQPYHHGYVAYRHTDEDIIYAADMVAESFAELK
                     KYI"
     gene            685..1512
                     /locus_tag="PLM68_05295"
     CDS             685..1512
                     /locus_tag="PLM68_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT54814.1"
                     /translation="MEKLIITAAICGAEVLKEHNPAVPYTVEECVREALTAYQAGASM
                     IHLHVRYDDGTPTQDKERFRVVMEAIAKACPGVIIQPSTGGAVGMTNDERLQATELNP
                     EMATLDCGTLNFGGDEIFQNTENTIKYFGQRMIERGIKPELEVFDKSMIEMALRLHKK
                     GYILSPMHFDFVMGVNGGIGGSLRDFLFLRGSIPSDATYTVAGLGRFEMPLAAAAIID
                     GGHVRVGFEDNVFLSKGVLAKSNGELVAKVVRMAVELGREIATPSEARQILGLPKRK"
     gene            1548..2591
                     /locus_tag="PLM68_05300"
     CDS             1548..2591
                     /locus_tag="PLM68_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOT54815.1"
                     /translation="MKKGNKYGLHRVIEPKGVLPQPANKLDNNMDEIYDNEILIDVRT
                     LNVDSASFTQIEEQAGGDKAEIARIMLDIVAKQGKHRNPVTGSGGMLLGVVEKIGEAL
                     EGKIDLKVGDKIATLVSLSLTPLRIDKIKDIRPDIDQVDIDGKAILFESGIYAKIPDD
                     MPETLALSALDVAGAPAQTAKLVKPGDTVLIIGAGGKSGMLCSYEARKRAGVTGKVIG
                     LCHSERSTKRMQDLGFCDHVFSADATQPVAVLEKIQELTKGQLCDITINNVNIPDTEM
                     TSILCTKDTGIVYFFSMATSFTKAALGAEGVGSDVTMIVGNGYTKGHAEITLQLLRES
                     EKLRKIFTELYAS"
     gene            2602..3849
                     /gene="ablA"
                     /locus_tag="PLM68_05305"
     CDS             2602..3849
                     /gene="ablA"
                     /locus_tag="PLM68_05305"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOT54816.1"
                     /translation="MKESRRKQLFPDVTDQEWDDWKWQVRNRIVNLEQLRKYISLTPE
                     EEEGVQESLKMLRMAITPYYLSLIDPDDPDCPIRKQAIPTKKETYRSPADLLDPLHED
                     EDSPVPGLTHRYPDRVLFLVTDQCSMYCRHCTRRRFAGQKDHEASGDRIQECINYIAR
                     TPQVRDVLLSGGDPLTMSDGRLESIIARLREIPHVEIIRIGTRTPVVCPQRITPELMA
                     MLRKYHPIWINTHFNHPNEITEKSRAACEMMADAGIPVGNQSVLLRGVNDCVHIMKEL
                     VHKLVLIRVRPYYIYQCDLSMGLEHFRTPVSKGIEIIEGLRGHTSGYAVPTFVVDAPG
                     GGGKIPVMPTYVISHAPGRMVLRNYEGVITTYTEPSDYKNYCDCIKCREKESLEGVSG
                     ILQGQKLSLEPSNLARKERNHKK"
     gene            3854..4870
                     /locus_tag="PLM68_05310"
     CDS             3854..4870
                     /locus_tag="PLM68_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54817.1"
                     /translation="MPFVDQIVRYGSLSVVGLGKNTGKTECLRYVLRRLSEMQKVVAV
                     TSIGVDGERIDQVTNTAKPEICLEKGMYFTTAASYYLNKRFTAEICQVEEFQTVLGKM
                     VTARALERGKMILAGPSDTGSLQEWVRKMPERFPVDVCLVDGAISRLSPASPVVTGSM
                     VLATGAAYSHDMKALAERTRYVYDTICLPAAPLDIVHKLAHVTNGIRGITPGGEVTNP
                     DVGQALLPEQIRQVIEKCGNRLYVPGVVSNLLLKALGEKKDVSSIELYIRDFSRIFAE
                     PRVYYTFLRKGGRIFQLFTTHLLAVCVNPVSPEGYRFHSEDLVDRIQQAIGVPVYDIK
                     KIET"
     gene            4867..6276
                     /locus_tag="PLM68_05315"
     CDS             4867..6276
                     /locus_tag="PLM68_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54818.1"
                     /translation="MTFAHFSERIEGLKYLTDRMELSGSRVRRYLMRESFCTHENVLA
                     LHFDILQKALDIVKDHSAMTGVMQLRHLFTQVHDIRATLDALRELRVLDEVELFEVKY
                     FSALCRDFSILCNTLGFPYETLQSLQEAFELLDPGGSGTVSFHLYDSYSQSLSDARKL
                     AASIDPEKEPDKWNALCDLITEEELLVRQKLSHALHLMHDVMQENFDRIARLEIWFAK
                     ATLAKRYNMVRPQIVPASAPCVLSIEGMVNPVIADTLEPVNKNFQPVSIMLCSGPCLL
                     TGANMSGKTVVLKTLALLQALTQFSFFVPARNASIPLVKEVFLVIGDKQDQYRGLSSF
                     AAEMLELDRIMAKVRKGTKVLVLADEPARTTNPAEGAAILCALVEFLDRYHTMSLIST
                     HYGDLGLSCRKLRVSGFDSKQVKGRLDPLMMNEHMNYSLIEEKGDSVPMEALHIARLL
                     GVDETFIRSAQQYVKNKEK"
     gene            6273..7829
                     /locus_tag="PLM68_05320"
     CDS             6273..7829
                     /locus_tag="PLM68_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOT54819.1"
                     /translation="MRESKLGLDFAKVKGARSLAAEIAAQVQHFVENYTTVAVERTLC
                     RLMGIDGVDSDGVPLPNIVVDAVREKGMLGEGVLRFLGNAIAVTGKTPLQTAESIARG
                     KFDFSLLDTSKNVNVSEALNPYIEQSIARIRNKRLAREEAIKKHGEGARPYLYVIVAT
                     GNIYEDAIQAQAAARQGADVVAVIRTTGQSLLDYVPYGPTTEGFGGTYATQENFRIMR
                     KALDEVIPETGRYVRLCNYCSGLCMPEIAVMGAFEGLDVMLNDALYGILFRDINMQRT
                     LVDQYFSRIINGFAGVIINTGEDNYLTTADAFEEAHTVLASDFINEQLALIAGIPEEQ
                     MGLGHAFEMDPELENAFLYELAQAQMTREIFPRAPLKYMPPTKFMTGNIFKGHIQDAL
                     FNMAGIWTGQGIQLLGMPTEAIHTPFMSDRYLAIENARYIFKSFKDIGKEVEFAQGGI
                     IRRRAGDVLDNAYQLLENVKEEGLFTALEKGIFAGVRRPREGGKGLDGVTRKSSGYVN
                     PFIELLKGKR"
     gene            7832..8623
                     /locus_tag="PLM68_05325"
     CDS             7832..8623
                     /locus_tag="PLM68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOT54820.1"
                     /translation="MSGGLYLTEMQDFDKTLDFKRVKPYGDTMNDGKVQLSFTLPVPA
                     GPEAIEAAKQLIRSMGMDNPQVVFSKELTPGFTFFNCYGSLRHTVNYTTIRVPKVDSR
                     VMDMYQVDEYIREHIGRKLVMVGASTGTDAHTVGIDAIMNMKGYAGHYGLERYAMIEA
                     HNLGSQVSNEELIAKAIQLEADAVLVSQTVTQKDIHIKNCTELIEMAEAEGIRERMIL
                     VCGGPRISHELAKELGFDAGFGVNTYADDVASFVVQEFVNRKQRK"
     gene            8647..>9192
                     /locus_tag="PLM68_05330"
     CDS             8647..>9192
                     /locus_tag="PLM68_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOT54821.1"
                     /translation="MDKNQIRETIARRASFELKDGDVVNLGIGLPTQVLKYLSPKVKV
                     IIQSENGCLGMGNPPEPGRENPDLIDAGGGAITLQPGASCFDSSLSFAIIRGGHVDIT
                     ILGSLQVDEKGNIANWSIPGIKTPGIGGAMDLITGAKKVIVTMEHTAKGEHKILENCT
                     LPLTAAGQVDRIITEMGVMDVT"
BASE COUNT         2510 a         2119 c         2458 g         2105 t
ORIGIN      
        1 cgctatcatc atcacaccgg ttgggcaccc tatggccgag aaggttcaaa tgcccaaacc
       61 cggttttctg gagggcgtgc gtgcgctggc cgacaaatac ggcaccgtgc ttgtgtttga
      121 cgaaatccgt agcggtttcc gctatgatct gggtggcgcc cagaaattgt tcggtgtcac
      181 ccccgacctt tcagtctttg gcaaggccat ggcaaacgga tatcccattg gagctgtcgt
      241 tggaaaggaa aagtacatga aagtgctggc agataaggtg ttcctttcat ccacattctt
      301 tcctaacagc ctggaacagg tggcagccct caagacaata gagatccttg aaagggataa
      361 catcctggat aaagtgaaag caaagggaga gttgttcggt aagcggctac aagagatcgt
      421 cagggatgtg ccttatgatg tggaagtgtc cgggataccc ctgatgccct tcatcacatt
      481 taaaaaggat gccgacaacc tgtacaagaa actccgtccc ggcttctata cggaacttat
      541 caggcgcggt gtattcctgc agccttacca ccacggatat gtagcatacc gtcataccga
      601 tgaagacatt atttacgcag cggacatggt tgcagaatca ttcgctgagc tgaaaaaata
      661 catctagcaa aatatcgacc tgtcatggaa aaattgatca ttaccgccgc aatatgtggc
      721 gccgaagtcc taaaggaaca caatcccgca gtcccataca ctgtcgagga atgcgtccgg
      781 gaagccctta cggcctacca ggccggtgcg agcatgatcc atctgcacgt ccgttacgat
      841 gacggtacgc ccactcagga caaggaacgt tttcgtgtgg tgatggaggc cattgcaaaa
      901 gcatgtcccg gagtgatcat ccagccttcc acgggaggag cagtgggcat gaccaatgat
      961 gaacggttgc aggccactga actgaaccct gaaatggcaa cactggattg cggaacgctc
     1021 aatttcggcg gggatgagat ttttcaaaac acagaaaata cgatcaagta cttcggtcaa
     1081 cgcatgattg agcgggggat caaacccgag ctggaagtat ttgacaagag catgattgaa
     1141 atggcactgc gtcttcacaa aaagggctat atcctgagcc ccatgcattt tgactttgtc
     1201 atgggagtca acgggggaat aggcggttcc ctaagagact ttttattcct gcgcggaagc
     1261 ataccttcag acgccacata cacggtagcc gggctgggtc gctttgaaat gcccctggca
     1321 gcggctgcca tcattgacgg gggccacgta agggtgggtt ttgaggataa cgttttcctg
     1381 tccaaaggcg ttctggccaa atccaacggg gagctggtgg ccaaggtcgt acgtatggcc
     1441 gtggaactgg gaagggaaat cgcaacacca tctgaagcac gtcagattct tggcctgcca
     1501 aaaagaaaat gagctaagaa tatcattaat ttatcaccat catatctatg aaaaaaggaa
     1561 acaaatatgg tctccacagg gtaattgagc ccaaaggcgt ccttccacag ccggcaaaca
     1621 agctggacaa caatatggac gagatctacg acaatgagat cctgatagat gtcaggaccc
     1681 ttaatgttga ctcggcaagt tttacccaaa ttgaggagca ggccggaggc gacaaggccg
     1741 aaattgcccg gatcatgctc gatattgttg ccaaacaagg caagcacagg aatccggtaa
     1801 ccggatcggg gggaatgttg ctgggagtgg tggagaaaat cggagaagcg ctggagggta
     1861 aaatcgacct gaaagtgggg gataagatcg ccacattggt ttctctttcc cttaccccgc
     1921 tccgtattga taagataaaa gacatccgtc ccgacataga tcaggtggat atcgacggga
     1981 aagccattct tttcgagagc ggcatatatg ccaagatacc ggatgatatg cccgagacac
     2041 ttgcgctttc tgcccttgat gtggcaggag ctcctgcgca gacagccaaa ctggtgaagc
     2101 ctggtgatac agtgctcatt ataggggcag gcgggaaatc gggcatgcta tgcagctatg
     2161 aagccaggaa aagggcaggg gttacgggta aagtgattgg cctctgccac agtgaacgct
     2221 ctaccaaacg tatgcaggac ctgggttttt gcgatcacgt gttttcagcc gatgccacgc
     2281 agccggtagc cgttctggaa aagatccagg agcttacaaa agggcagtta tgtgatataa
     2341 ccatcaacaa tgtgaacatt cccgataccg agatgacttc catcctctgt accaaagata
     2401 ccgggattgt ttactttttc tccatggcga ccagtttcac gaaagcggcg cttggggcag
     2461 aaggagtcgg ttccgatgtc accatgattg taggaaacgg ttatacgaaa ggtcacgcag
     2521 agatcaccct gcagttgctc agggaaagtg aaaagctccg caagatcttt acagaacttt
     2581 acgcatcata attttacgca aatgaaagaa tcaagaagaa aacagctgtt tcccgatgtt
     2641 acggaccagg aatgggatga ctggaagtgg caggtccgaa accgtatagt caaccttgaa
     2701 cagctcagga aatatatcag cctgaccccg gaagaggaag aaggtgtcca ggagtccctg
     2761 aaaatgctcc gcatggctat aacgccttat tacctgagcc tgattgatcc tgatgatcct
     2821 gattgtccca taaggaagca ggccattcct acaaaaaagg aaacataccg ttcccctgca
     2881 gacctgctgg atccgctgca tgaagacgaa gattcaccgg tacccggcct gacacacagg
     2941 tatcccgaca gggtcctgtt cctggtcacc gaccagtgtt ccatgtattg ccgtcattgc
     3001 acaagacgtc gctttgccgg acagaaggat cacgaagcat ccggtgacag gatccaggag
     3061 tgcatcaatt atattgcgcg tacaccccag gtaagggatg tcctgctctc cggaggggat
     3121 ccgctgacca tgagcgatgg ccgtcttgaa tctatcattg cccgcttaag ggagatcccg
     3181 catgtggaga tcattcggat tggcacacga acaccggtcg tttgcccgca aaggatcact
     3241 ccggagctga tggcaatgct ccgcaaatac catcctattt ggatcaatac gcatttcaac
     3301 catcccaacg agataaccga aaaatccagg gctgcctgcg agatgatggc agatgccggt
     3361 ataccagttg ggaaccagag tgtcttgctg cgtggagtga atgattgcgt tcatattatg
     3421 aaagaactgg ttcacaagct ggtactcatc cgtgtccgtc cttactatat ttaccagtgt
     3481 gacttgtcca tgggtctgga acatttcaga accccggttt ccaaggggat agagataatt
     3541 gagggattac gcggacatac ttccggctat gcggttccga cttttgtggt ggatgctccg
     3601 ggaggaggag gaaagatccc ggtaatgcca acgtatgtga tcagtcatgc tccgggtcgt
     3661 atggtgctgc gcaattacga gggtgttatc actacataca cagaaccatc tgactataag
     3721 aactattgcg attgcattaa atgcagggaa aaagagagcc tggaaggggt ctcgggtata
     3781 ctgcaaggtc agaagctatc ccttgaaccg tctaatcttg caagaaaaga acgaaatcac
     3841 aagaaatgat taaatgcctt ttgtagatca gatagtccgt tacggcagcc tttcggtcgt
     3901 gggtttgggg aaaaataccg gaaagacaga atgtctcagg tatgtattga gacggttatc
     3961 tgaaatgcag aaagtggtgg ctgtcacatc cataggtgtt gatggagaga ggatcgacca
     4021 ggtcactaat acggccaagc ctgaaatatg cctggaaaag ggtatgtact tcactaccgc
     4081 tgcttcctat tacctgaaca aacgctttac ggcagagatc tgccaggtgg aagaattcca
     4141 gactgtattg ggcaagatgg tcacagccag ggcgctggaa agggggaaaa tgatcctggc
     4201 aggtccttcc gacactggtt ccctgcagga atgggtcagg aagatgcctg aaagattccc
     4261 tgttgatgtg tgtcttgtcg acggggccat atcacgtctg agcccggcat cccccgttgt
     4321 aaccgggtca atggttttgg ccacaggagc ggcttattcg catgatatga aagcccttgc
     4381 agaaaggaca cgttatgtgt atgataccat atgcctgcct gccgcacccc tggacatagt
     4441 acacaaactg gcacatgtca ccaacgggat aaggggaatt actcccgggg gagaagtgac
     4501 caatccggat gtgggacagg ctttgcttcc ggagcagatc agacaggtga ttgaaaagtg
     4561 cggtaacagg ctgtatgttc cgggggtagt aagcaatttg ctgctgaaag ccctgggaga
     4621 aaaaaaggat gtaagcagca tagaattgta tatcagggat ttttcccgga tttttgcaga
     4681 accgcgtgtt tattacacgt ttctccggaa aggagggcgg atcttccagc ttttcactac
     4741 ccatttgctg gctgtctgcg tgaatcctgt ctcccctgaa ggataccggt ttcattctga
     4801 ggatcttgtt gacaggatac agcaggcaat tggggtgccc gtttacgata taaaaaagat
     4861 tgaaacatga cttttgcgca tttttcggaa cgaattgaag gccttaagta cctgactgat
     4921 agaatggaac tatccggctc gcgtgttcgc cgttacctga tgcgggagtc gttttgcacc
     4981 catgaaaatg ttcttgcatt acacttcgat atattacaga aggcgcttga tatagtgaaa
     5041 gaccactctg ccatgactgg tgtgatgcag ctacgccatc tcttcacaca ggttcatgat
     5101 atcagggcaa ccctggatgc cctgcgcgaa ctacgtgtgc ttgacgaggt ggagttgttt
     5161 gaagtcaagt atttctcagc gttgtgcagg gatttcagca tactttgcaa cacacttggt
     5221 tttccatatg aaaccctgca gtcccttcag gaggcatttg agctgctgga tcccggcggg
     5281 agcggaaccg tatcgttcca tctgtacgat tcctacagcc agtccttgtc agacgcccgt
     5341 aaacttgctg cttccatcga tccggagaag gagccggaca agtggaacgc cctttgcgat
     5401 ctgattactg aggaagagct gctggtcagg cagaagctct cccatgcctt gcatctgatg
     5461 catgatgtga tgcaggagaa ttttgaccgt atagcccgcc tggagatatg gtttgccaaa
     5521 gccaccctgg caaaacgcta caacatggtc agaccacaga tagtgcctgc ctctgctccc
     5581 tgtgtcttaa gcatagaggg catggtgaac cctgtcatag cagatacact ggaaccggtc
     5641 aataaaaact ttcaacccgt atccataatg ctctgcagcg gcccctgcct tctgaccggc
     5701 gctaacatgt ccggcaagac ggtggtttta aagactctgg ccttgctgca ggcactcacg
     5761 cagttttctt tctttgtacc agcccggaat gcatcaattc cccttgtaaa ggaagttttt
     5821 cttgtcatag gcgacaaaca ggaccaatac cggggattat catcatttgc ggcagaaatg
     5881 ctcgaattgg accggataat ggctaaagtg agaaaaggaa caaaagtgct tgttttagcc
     5941 gatgaacccg ccaggaccac caaccctgcc gaaggagctg ccattttgtg tgccctggtt
     6001 gaatttctgg accgttacca taccatgtcc cttatcagta cccattacgg ggacctgggc
     6061 ctgtcctgcc gtaaattgcg cgtaagcggc tttgacagca aacaggtaaa aggccgcctg
     6121 gacccgttga tgatgaatga acatatgaat tacagcctga ttgaggaaaa aggcgattca
     6181 gtacctatgg aagcattgca catagcgcgt ctgctgggcg tggatgagac ttttatacga
     6241 tctgcacaac aatatgtgaa aaacaaagaa aaatgagaga atcgaaatta ggtcttgatt
     6301 ttgccaaagt aaaaggtgcc cggagtcttg ctgctgaaat agccgcccag gtacaacact
     6361 ttgtggaaaa ttacaccacg gtggcagtag aaaggacgct gtgccggttg atgggaattg
     6421 acggtgttga cagcgatgga gtacctttac ccaacatcgt ggtggatgcg gtccgggaaa
     6481 agggaatgtt gggcgagggg gtgctccgtt tcctgggcaa tgccattgcc gtaacgggca
     6541 agactccact gcagacagcc gaatcgattg ccaggggaaa attcgatttt tcactcctgg
     6601 ataccagcaa gaacgtgaac gtttccgagg ctctcaatcc gtacatagag caaagtattg
     6661 caaggataag gaacaagagg ctggccaggg aagaagccat aaaaaagcac ggggaaggag
     6721 cccgtcctta tctttatgtg atcgtagcca ccgggaatat ctatgaagat gccatccagg
     6781 cacaggccgc tgcccgccag ggagccgacg ttgtggcggt tatccgcacc acaggccagt
     6841 ctcttcttga ttacgtccct tatgggccca ctaccgaagg ttttggcgga acctatgcca
     6901 cccaggagaa tttccgtatc atgcgcaagg cgctggacga ggtcattccg gaaacgggcc
     6961 gttatgtcag gttgtgcaat tactgctctg gtttgtgtat gcccgagatt gccgtcatgg
     7021 gagcctttga aggactggat gtgatgctga acgatgcgct atacggcatt cttttccgtg
     7081 acatcaacat gcaaaggaca ttggtggacc agtatttttc ccggataatc aatggatttg
     7141 caggggtgat aatcaatacc ggagaggaca attacctaac gacagccgat gcgtttgaag
     7201 aagcacatac ggtccttgcc tcggatttca tcaatgagca actggcgctg atagcaggga
     7261 tcccggaaga acagatgggc ctgggacatg cctttgaaat ggatcccgag ctggaaaatg
     7321 ccttccttta tgaactggct caggcacaaa tgacccggga aatatttcca cgggcacccc
     7381 tgaaatacat gccgccaaca aaattcatga ccggcaacat attcaaagga catatccagg
     7441 acgccctgtt caacatggcc ggcatctgga ccggacaggg tattcagctc ctgggaatgc
     7501 ccacagaggc catccacact ccattcatga gtgacaggta cctggccatt gagaacgcca
     7561 ggtatatatt caaaagtttc aaggacatag gaaaagaagt cgaattcgca caagggggca
     7621 tcatacgccg gcgtgcaggg gacgtgttgg acaatgctta tcaattgctt gaaaacgtga
     7681 aggaagaagg actctttacc gctttggaaa aaggcatctt tgccggagta agaagaccca
     7741 gggaaggggg gaaaggactt gacggggtga cccggaaatc ttcgggttat gtgaatccat
     7801 ttattgaatt gctaaaagga aaaagataat catgagcgga ggtttgtatt taacagaaat
     7861 gcaggatttt gataagactc tggatttcaa gagggtgaaa ccctatggag ataccatgaa
     7921 cgatggcaag gtccagctaa gtttcacatt acctgtacca gctgggccgg aagcgataga
     7981 ggcggccaaa caattgatcc gcagtatggg aatggataac ccccaggtgg tgttttccaa
     8041 ggaactgacc ccgggtttca ccttcttcaa ctgttacgga agtctccgtc atacagttaa
     8101 ttatactacc atccgcgtac ctaaggtgga tagcagggtc atggatatgt accaggtgga
     8161 tgaatacatc agggaacaca tagggcgtaa actggtcatg gtgggagcca gcaccggaac
     8221 ggatgcccat accgtgggaa tagatgccat catgaacatg aaaggatatg ctggccatta
     8281 tggtttggaa cggtacgcaa tgatagaagc acataacctt ggttcacagg tttctaacga
     8341 agagctcatt gctaaggcga tccagcttga ggcagatgcc gtgctggtat cacagacagt
     8401 cacccagaaa gacattcata taaaaaactg tacggaactc atagaaatgg cagaggccga
     8461 agggataagg gaacgcatga ttttagtatg cggaggaccc aggatctctc atgaactggc
     8521 caaggagctg ggctttgatg ccggctttgg agtcaacacc tatgccgatg atgtggcttc
     8581 ttttgtagtc caggaatttg taaacagaaa acaaagaaaa taaaagaata ataaaagcag
     8641 aaaatcatgg acaagaatca aatcagggaa accattgcac gtcgcgcatc ctttgaattg
     8701 aaagacggag acgttgtaaa cctgggcatt ggattgccca cacaggtctt gaaatacctg
     8761 tcgccaaaag tgaaagtgat catccaatca gagaacgggt gcctgggtat gggaaatccg
     8821 ccggaacccg gaagggaaaa tcctgatctg atagatgccg gtggaggagc tatcaccttg
     8881 caaccgggag cttcctgttt tgattcttcc ctttcctttg cgatcatccg tggcgggcat
     8941 gtcgacatta ccatactggg ttcactgcag gtggatgaaa aagggaacat cgccaattgg
     9001 tcgatacccg ggataaagac accgggcatc ggaggagcca tggacctgat aaccggagcg
     9061 aaaaaggtga tcgtgaccat ggaacataca gcaaaagggg agcataagat tctagaaaac
     9121 tgtaccctgc ctctcacggc tgccggccag gttgatcgta tcattacgga aatgggcgtt
     9181 atggatgtga ct
//
LOCUS       DAOSOF010000073         9158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7524_length_9158_cov_4.759310, whole genome shotgun sequence.
ACCESSION   DAOSOF010000073 DAOSOF010000000
VERSION     DAOSOF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7524_length_9158_cov_4.759310"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLM68_05335"
     CDS             <1..192
                     /locus_tag="PLM68_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54822.1"
                     /translation="PNRNKVASVTWDQVRAIAENKMEDMNAFSLNAAMSMVAGTARSM
                     GITVTGTAPEGVIKTSMLK"
     gene            189..890
                     /gene="rplA"
                     /locus_tag="PLM68_05340"
     CDS             189..890
                     /gene="rplA"
                     /locus_tag="PLM68_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT54823.1"
                     /translation="MSKLTKNQKVVYAKVDSEKQYRLSEACELIGQTSFTRFDASVDI
                     AVRLGVDPRKANQMVRGVVTLPHGTGKQVRVLVLCTPDKEEEAKNAGADHVGLDDYID
                     KIKGGWTDVDVIICTPSVMAKIGPIGRILGPRGLMPNPKTGTVTMEIGKAVQEVKAGK
                     IDFKVDKTGIIHASIGKVSFEGKKLQDNVQEFINTVIKLKPQALKGTYIKSIYLSTTM
                     GPGLAIDVKSGTAAE"
     gene            905..1426
                     /gene="rplJ"
                     /locus_tag="PLM68_05345"
     CDS             905..1426
                     /gene="rplJ"
                     /locus_tag="PLM68_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT54824.1"
                     /translation="MKKEQKGLIIESLAAQLKATPDFYVVNIEGLDAGKTSLLRRKCF
                     AQNIKMVVVKNTLLKRTLEQAGVEEMKLLFPILEGSTAVMFTENVNAPAKLIKSFGQA
                     NGKPVLKGALVQECAYVGEDQLENLINIKSREELIGDIAGLILSPAQRLVSAIEAAGS
                     NMAGIVKTLAERE"
     gene            1465..1842
                     /gene="rplL"
                     /locus_tag="PLM68_05350"
     CDS             1465..1842
                     /gene="rplL"
                     /locus_tag="PLM68_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT54825.1"
                     /translation="MAELKQMAEAIANLTIKEAIELANILKDEYGIEPAAAAVAVAAA
                     PGAAGPAAEAEQTQFDVILTSAGQAKLQVIKVVKEITALGLKESKDLVDGAPKAIKEK
                     VSKAEAEQIKSQIEEAGGEVEIK"
     gene            1947..5765
                     /gene="rpoB"
                     /locus_tag="PLM68_05355"
     CDS             1947..5765
                     /gene="rpoB"
                     /locus_tag="PLM68_05355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT54826.1"
                     /translation="MPQRANIQRINFAKSKTLLDHPDFLEVQLKSFQDFFQLETTPEN
                     RKNEGLFKVFQENFPITDTRNNFVLEFIDYFIDPPRYSIEECLDRGLTYSVPLKAKLK
                     LYCTDPEHEDFETIIQDVYLGTIPYMTPRGTFIINGSERIVVSQLHRSPGVFFGQSIH
                     TNGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFGLANEVKVTRANLKKNLGRQLAARVLKSWVEDFVDEDTGEVVTIERNEIILDRKTI
                     LEEEHIELILDSSTATILLHKEDANVGDYAIIHNTLQKDPCNSAKEAVIYIYRQLRNS
                     EPPDDATALDVIDKLFFSSKRYDLGDVGRYRLNRKLSLNTPSDIKVLTKEDIIAIISH
                     LIQLINSKATVDDIDHLSNRRIRTVGEQLANQFSVGLSRMARTIRERMNVRDNEVFTP
                     IDLINAKTLSSVINSFFGTSQLSQFMDQTNPLAEMTHKRRLSALGPGGLSRDRAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYARINDLGFIETPYRKVEDGKVDLSAKG
                     YIYMSAEEEEERMVAQANVNLDDDGMILDERIKCRYEADYPVVTKEDVHLIDVSPNQI
                     ASIAASLIPFLEHDDANRALMGSNMMRQAVPLISPEVPIVGTGLESTVTKDSRTQITA
                     EGKGEVVFVDANEIHIRYERTDDEKFVSFSPDITVYKLPIYRKTNQNTSVNLKPLVRK
                     GQKVVQGQILTEGYATQNGELALGRNLKVAFMPWKGYNFEDAIVLSERMLREDVFTSI
                     HVDEYIMEVRDTKRGMEELTSDIPNVSEEATKNLDENGIIRIGAYIEPGDILIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKASPSLYGTVIEKRLFSRVMKEGTKKIKP
                     IAKMQIQQADEKFKARFDKLEKSFLDKLESLLKDKISPGITNLYGEMVIPKDSRFTRE
                     IISGISYENVNPTKWTNDKHTNTLIRKLINNYLISFKELDAELKRVKYSLTIGDELPT
                     GIMQLAKVYIAKKRKIRVGDKMAGRHGNKGIVAKVVRDEDMPFLDDGSIVDVVLNPLG
                     VPSRMNLGQIYETVLGWAGKELGLTFSSPIFDGASLEEITHYTDKANVPKYGKTYLRD
                     GGTGDYFDQPATVGMIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGE
                     MEVWALEAFGAANILQEILTVKSDDVAGRSRAYESIVKGDTMPAAGIPESLNVLLHEL
                     RGLGLSVNLE"
     gene            5776..>9158
                     /gene="rpoC"
                     /locus_tag="PLM68_05360"
     CDS             5776..>9158
                     /gene="rpoC"
                     /locus_tag="PLM68_05360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT54827.1"
                     /translation="MAYKKDSKLKSNFKKITIGLASPEEILDMSHGEVLKPETVNYRT
                     YKPERDGLFCERIFGPSKDYECHCGKYKRIRYRGITCDRCGVEVTEKKVRRERMGHIS
                     LVVPIAHIWYFRSTPNKIGYLLGIPSKKLEAIIYYERYVVIQPGIKAQDGINELDFLT
                     EEEYLNVLDTLPRENQMLDDEDPNKFIAGMGAEAIYKLLVRLNLDDLSYDLRHKADVE
                     TSQQRKADALKRLNIIEAFRDSKEINKPEWMIMKVIPVIPPDLRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTEANR
                     TLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELNMHECGLPKDMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPMIWDILENVMKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGHAAILEAQMLMLGSHNILNPANG
                     APITVPSQDMVLGLYYITKLKSKAKGEGMTFYSPEEVIIAYNEKVADMHARIKLRLNK
                     TIVRYFKDESNPSGITSTFMADPATGNHIIDTSVGRVLFNQVVPKEVGYINMILTKKS
                     LRDIINLVLKETGVARTAQFLDNIKEIGYSMAYKGGLSFNLDDVLVPEEKATLINKAY
                     EEVEQIRENYNLGFLANNERYNKVVDTWTTVNVRLSNIVEEKLKEDKEGFNPIYMMLD
                     SGARGSKEQIRQLSGMRGLMGKPQKSGASSAEIIENPILANFKEGLSVLEYFISTHGA
                     RKGLADTALKTADAGYLTRRLVDVSHDVIINEEDCGTLRGLVATAIKNKDKIVESLYD
                     RILGRTSVHDIFDPLNGKLILESGEEITEDVADQISRLPIEQVEIRSVLTCESKKGVC
                     AKCYGRNLATGRMVEKGEVVGVVAAQSIGEPGTQLTLRTFHQGGTASTFQSESEIVAR
                     YNGILEIDELRTLNKVSPTGETQTIVIGRTAEMRIVDSNTRITLSTYNIPYGANLYFK
                     DGDEVREKDKICEWDAYNAVTIAELDGTVAFDSLIEGVTYREEAADEYFLHRDKVIIE
                     SRDRSRNPTLKIMKNNVELKQYNLPVGAHIMVNEKQEIKA"
BASE COUNT         2730 a         2167 c         2214 g         2047 t
ORIGIN      
        1 ccaaaccgta acaaagtagc ttcggttacc tgggatcagg tccgtgccat tgctgaaaac
       61 aaaatggagg acatgaatgc cttcagcttg aatgcagcaa tgagcatggt ggcaggtact
      121 gcacgcagta tgggtatcac cgtgaccgga actgcccctg aaggtgttat taaaacttct
      181 atgttgaaat gagtaaactg actaaaaatc aaaaagtagt atacgccaag gtcgattctg
      241 aaaaacaata cagattgtcc gaggcctgtg aactgatcgg gcagaccagt ttcaccaggt
      301 ttgacgcttc ggtggacatt gctgtgcgtc tgggcgttga cccgcgtaaa gcaaaccaga
      361 tggttcgcgg tgttgtgact cttccgcatg gaacaggaaa gcaggtgcgt gtattggtgc
      421 tttgtacacc cgataaggaa gaagaagcaa agaatgcagg tgctgaccat gtgggtcttg
      481 acgattacat tgataagata aaaggaggat ggacagatgt tgatgtaatc atctgtaccc
      541 cttctgtcat ggccaaaatc ggtcctattg gacggatcct tggtcctcgc ggactgatgc
      601 cgaatccgaa aaccgggacc gtaaccatgg agatcggaaa ggctgttcag gaggtcaagg
      661 ctggaaagat tgactttaaa gtagataaaa ccgggatcat ccatgcttcc atcgggaaag
      721 tatcctttga aggcaaaaag cttcaggata atgtccagga gtttatcaac acggtcatca
      781 aactgaaacc tcaggccctc aagggtactt atatcaagag catatacctc tccacaacca
      841 tggggccggg tcttgcaatt gatgtcaaat caggtactgc tgctgaataa agaaattgtg
      901 tatcatgaag aaagaacaaa aaggacttat catagaaagt ttggcagctc agctcaaggc
      961 aacccctgat ttttatgttg tgaacattga aggacttgat gccgggaaaa catccctgct
     1021 ccgtcgtaaa tgctttgcac agaacataaa gatggttgtt gtcaagaaca ccctgctaaa
     1081 acgtaccctg gaacaggctg gtgttgagga aatgaaactg cttttcccaa tacttgaagg
     1141 ctccacggct gttatgttca ctgaaaatgt caatgccccg gcaaagctga tcaaatcatt
     1201 tggtcaggca aacggaaaac ccgttctcaa gggggctttg gttcaggaat gcgcctacgt
     1261 tggggaagat caactggaga accttatcaa tatcaaatcc cgtgaagaac tcataggaga
     1321 tattgcaggc ctgatccttt cccctgcaca gagactagta tcggctatcg aagcagccgg
     1381 aagcaatatg gccggtattg ttaaaaccct ggccgaacgt gaataatcat taacaggaaa
     1441 acaaaatttt aattataatc aatcatggca gaattgaaac aaatggcaga agccattgct
     1501 aatctcacca ttaaagaagc tattgagtta gcaaacatcc tcaaagacga atacggaatt
     1561 gaacccgccg ccgctgctgt tgcagttgcc gcagctcccg gagctgccgg acctgctgct
     1621 gaagcagaac aaacccaatt tgatgtaata cttacctcgg ccggacaagc caagctgcaa
     1681 gttatcaaag tggttaagga aattaccgct ctcggactca aggaatccaa agatctggtt
     1741 gacggtgctc ccaaggctat aaaggaaaaa gtaagcaagg ccgaggctga acaaatcaaa
     1801 tcacaaattg aagaggcagg cggcgaagtt gaaattaaat aaactctctt cccgtacaaa
     1861 tcgggaacgg taccaggttt aggaccccca aggggttcta agcctttttg tcgttaaaac
     1921 gtttattttc aactaatcct taaaaaatgc ctcaaagagc taatattcag cgaattaatt
     1981 tcgcaaaatc aaaaaccctc cttgatcatc cagatttttt ggaggtacaa ttgaaatctt
     2041 tccaggattt cttccagctg gagacaacac cggaaaacag gaaaaatgaa ggattgttta
     2101 aggtatttca ggaaaatttt cccataaccg atacccgcaa caattttgtt ctggaattca
     2161 tagactattt tattgatccg cctcgttatt ccattgagga gtgtcttgac agggggctga
     2221 catacagcgt ccccctaaag gctaaactta aactttactg caccgatcct gaacacgagg
     2281 attttgaaac gatcatacag gacgtgtacc tgggaaccat tccttacatg acaccaaggg
     2341 gaacctttat aatcaatggt tccgaacgca tcgtcgtatc acagcttcac cgttccccgg
     2401 gcgtgttttt cggacaaagt atacatacca acgggacaaa actttattct gcccgtatca
     2461 tcccgttcaa gggatcatgg attgaattcg caacggacat caataacgtg atgtacgcat
     2521 atattgacag gaaaaagaaa cttcctgtca caaccctcct tcgtgccatt ggatttgaaa
     2581 gtgacaagga tatcctggaa atattcggtc tggccaatga agtcaaagtg acccgggcca
     2641 atctgaaaaa gaacctgggc agacagctgg ctgcacgggt gctgaaatcc tgggtagaag
     2701 attttgtaga cgaagatacc ggggaagttg ttaccataga acgtaatgag atcattctgg
     2761 acaggaagac catcctggaa gaggaacaca ttgaactgat cctcgactcc agcacggcta
     2821 ccatcctgct gcataaggaa gatgccaacg tgggagatta tgccatcatc cacaataccc
     2881 ttcagaagga tccatgcaac tcggctaaag aagccgtcat atacatatac aggcaattac
     2941 gcaactccga accaccagac gatgcaacag cactggatgt gatcgacaag ctcttctttt
     3001 catccaaacg atatgacctg ggtgatgttg gacgttaccg cctgaaccgc aaactgagcc
     3061 tgaacactcc ttcggatatc aaggttttga ccaaggaaga tatcatagcc ataataagcc
     3121 acctgataca gctgatcaat tcaaaagcca cagtggacga tattgaccac ctgagcaacc
     3181 gccgcatacg tactgtggga gaacagctgg caaatcagtt cagcgttggt ctttcccgta
     3241 tggcccgcac tatcagggaa agaatgaatg tacgcgacaa cgaggtgttt acacccatag
     3301 accttatcaa tgccaagaca ctgtcctcgg tcataaactc gttctttggg acatcacagc
     3361 tgtcccagtt catggaccag acaaacccgc tggcagaaat gacacacaag cgccgtcttt
     3421 cggctcttgg ccctggcggg ctctcaagag accgcgccgg atttgaagta cgcgacgttc
     3481 actacaccca ctacggaaga ctgtgtccca tagagacacc ggaaggcccc aatatcggcc
     3541 ttatttcttc cctgtgcgtc tatgcccgta tcaacgacct gggattcata gagacacctt
     3601 accgtaaagt cgaagacgga aaggtggacc tgtctgccaa aggttacatt tacatgagtg
     3661 ctgaagagga agaagaacgt atggtggctc aggccaatgt gaacctggat gatgacggga
     3721 tgatcctgga tgaaaggatc aaatgccgtt acgaagcaga ttatcctgtg gtcaccaaag
     3781 aagatgtgca cctgatagac gtctcaccca accagatagc atccatagcc gcttcgctca
     3841 ttcctttcct ggaacatgac gatgcaaaca gggccctcat gggatccaac atgatgcgcc
     3901 aggccgtgcc tcttatcagc ccggaagttc ctattgttgg gactggcctt gaaagtaccg
     3961 tcactaaaga cagccgcacg cagatcactg ccgagggcaa gggtgaagtc gtgtttgtag
     4021 atgccaatga gatacacatt cgttacgaaa ggaccgacga tgaaaaattc gtaagcttca
     4081 gcccggatat caccgtctac aagctcccga tatacaggaa aacaaaccag aatacctctg
     4141 tcaacctgaa accattggta agaaagggtc aaaaagtggt ccagggacag atcctgacgg
     4201 aaggatatgc cacccagaac ggagaacttg ccctgggacg caacctgaaa gtggctttca
     4261 tgccctggaa agggtataat tttgaagatg ccattgtcct ttcggaaagg atgctgcgcg
     4321 aagacgtgtt cacttctatc catgtggatg aatacataat ggaagtccgt gatacaaagc
     4381 gtggtatgga agaactgacc agcgacatac ccaacgtttc ggaagaagcc acgaaaaacc
     4441 tggacgaaaa tgggatcatc cgcataggtg cttacattga accgggagac atcctcatag
     4501 gaaagatcac gccaaaaggc gaaagtgatc cttcccctga agaaaagttg cttcgtgcca
     4561 ttttcggaga caaagcagga gatgtgaagg atgcttcact caaagcatcc ccttcccttt
     4621 acggtactgt aattgaaaaa cgacttttct ctcgcgtgat gaaagaggga accaagaaga
     4681 tcaagcctat tgccaagatg cagatacagc aggcagacga gaaattcaaa gcccgctttg
     4741 acaagcttga gaaaagcttc ctggacaaac tggaatccct gctcaaggat aaaatatcac
     4801 cgggtatcac caatctctac ggagagatgg tcattccaaa ggacagccgt ttcaccaggg
     4861 aaatcatttc gggtatttcc tacgagaatg tgaatccgac caaatggacc aatgataaac
     4921 acaccaatac ccttatcaga aaactgataa acaattatct gatcagcttc aaggagcttg
     4981 atgcggaact taagcgtgtc aaatacagcc ttaccattgg tgacgaactc cccaccggga
     5041 tcatgcagct tgcaaaggtt tacattgcca agaagcgtaa gatccgtgta ggagataaaa
     5101 tggccggccg acacgggaac aagggcattg tagctaaagt cgtgcgcgac gaagacatgc
     5161 ctttcctgga tgatggctcc atagtggatg ttgtccttaa tcccctgggt gttccttccc
     5221 gtatgaacct gggacagatt tatgaaacag tgctgggctg ggccggaaaa gaactcggcc
     5281 ttactttctc ttctcccatc tttgatggcg catcactgga agagatcacg cactataccg
     5341 ataaagccaa cgtgcccaaa tatggcaaga catacctgcg cgacggaggt acgggtgatt
     5401 attttgacca gccggcaacc gtgggtatga tctacatgct caaactggga catatggtgg
     5461 acgataagat gcatgcccgt tcaataggtc cctattcact cattacccag caacccctgg
     5521 gtggtaaagc ccagttcggg ggacaacggt ttggggagat ggaggtttgg gccctggaag
     5581 cattcggtgc agccaatatc cttcaggaga tcctgacagt caaatcggac gacgtggccg
     5641 gaaggtcccg tgcctatgag tccattgtca aaggggatac catgcctgca gcagggattc
     5701 ccgaatcact gaatgtattg ctccatgaat tgcgcggtct gggactgagc gttaacctgg
     5761 aataaaaatt tgattatggc ttacaagaaa gatagcaagt taaaaagcaa cttcaagaaa
     5821 atcaccatag gactggcttc tccggaagaa atcctggaca tgtcacatgg agaagtatta
     5881 aagccggaaa ccgttaacta ccgtacttac aaaccggagc gcgacgggtt gttctgcgaa
     5941 cgcatattcg gcccttcaaa ggattacgag tgccactgcg gtaaatacaa acgcatacgt
     6001 tatcgtggta tcacctgtga ccgttgcggg gtggaagtaa ccgagaaaaa agtgcgcagg
     6061 gagcgcatgg gacacatatc actggttgtt cccatagctc atatatggta tttcagatcg
     6121 accccgaaca agataggtta cctgctcggg atcccttcca aaaaacttga agctatcata
     6181 tattatgaaa gatacgtggt cattcagccc ggcatcaaag cccaggacgg tatcaatgag
     6241 ctggatttcc tcacagaaga ggaatacctg aacgtactgg ataccctccc cagggaaaat
     6301 cagatgctgg acgatgaaga ccccaacaaa ttcatagctg ggatgggagc cgaagccatt
     6361 tacaaactcc ttgtccgtct gaatctggat gatctttcct atgacctgcg tcacaaggca
     6421 gatgtggaaa cctcgcagca gagaaaagct gatgcgctga aaaggctcaa catcattgaa
     6481 gctttccgcg actccaaaga gatcaacaaa ccggaatgga tgattatgaa agtgatccct
     6541 gtgatccctc ccgatctgcg gccacttgtt cctcttgatg gaggtcgttt tgccacatcg
     6601 gacctgaatg atctgtaccg tcgggtgatc atccggaaca accgcctcaa aaggctgata
     6661 gagatcaaag ccccggaagt gatccttagg aatgagaaga ggatgttgca ggaggccgtt
     6721 gactccctgt ttgacaactc caggaagtcc aatgccgtta aaacggaagc caacagaacc
     6781 cttaaatcct tatctgacag tttaaaggga aaacagggcc gtttccgtca gaacctgctt
     6841 ggtaaacgcg tggactactc ggcccgttca gtcattgtgg taggtccaga attgaatatg
     6901 cacgaatgcg gtctgcccaa agatatggcg gctgaactgt acaaaccgtt catcatcaga
     6961 aaactcattg aaagaggtat cgtcaagacc gttaaatccg ccaagaagat tgtggaccgg
     7021 aaagatccca tgatctggga tatcctcgaa aatgtgatga aaggacatcc tgtgctgctg
     7081 aaccgtgccc ctaccctgca ccgtctgggt atccaggcat tccagcccaa gcttatcgag
     7141 ggtaaagcca tccagcttca ccccctggca tgtaccgctt tcaatgccga ctttgacggc
     7201 gaccagatgg ccgtgcacct gccccttggc catgcagcta ttctggaagc gcaaatgctc
     7261 atgctcggct ctcataacat cctgaatccg gcaaatggtg cccccattac cgttccttca
     7321 caggacatgg tgcttggcct gtactatatc accaaattga aaagcaaggc caagggagaa
     7381 ggaatgacct tctacagccc ggaagaagtg atcatcgcct acaacgaaaa ggttgcggac
     7441 atgcacgcac gcatcaagct ccgtctgaat aagaccatag tccgttattt caaggatgag
     7501 agcaatcctt ccggtataac ctccactttc atggcagatc cggcaaccgg gaatcacatt
     7561 attgacacat cggtaggtcg tgttctcttt aaccaggtgg taccgaagga ggtgggatac
     7621 ataaacatga tcctgaccaa aaaatccctg cgtgatatca tcaatctggt ccttaaggaa
     7681 accggagtgg cacgtacggc tcaattcctt gacaacatca aggaaattgg atatagcatg
     7741 gcctacaagg gcggactttc cttcaacctg gacgatgtcc tggtaccaga agaaaaagca
     7801 accctgatca ataaagccta tgaggaagta gagcaaataa gggaaaatta caatttggga
     7861 ttccttgcca acaacgaacg ttacaacaag gtggtggaca cgtggaccac tgtcaacgtg
     7921 cgtctttcaa acattgtgga ggaaaagctc aaggaagaca aagaagggtt caatcccata
     7981 tacatgatgc tggattccgg agcacgcgga tccaaggaac agatccggca gttatccggt
     8041 atgcgtggtc tgatgggtaa acctcagaaa tcgggagctt ccagtgccga aatcattgaa
     8101 aaccccattc tggccaattt caaggaaggt ttgtccgttc tggaatactt catttccacc
     8161 cacggtgccc gtaaagggct ggcagatacc gctctgaaaa cagcagatgc cggttacctg
     8221 acacgccgcc ttgtggatgt atcgcatgat gtgatcatta acgaagaaga ctgcgggaca
     8281 ctgcgtgggc ttgtggcaac tgccatcaag aataaagaca agattgtcga gtctctctat
     8341 gaccgtatcc tgggacgtac cagcgtacac gatatatttg accccctcaa tggaaaattg
     8401 atcctggaat ctggagaaga gataacggaa gacgtagccg accagatatc cagacttccc
     8461 attgagcagg tggaaatacg cagcgtgctg acctgtgaat caaagaaagg tgtctgcgcg
     8521 aaatgctacg gaaggaacct ggccaccggt cgtatggtgg aaaaaggtga agtagtggga
     8581 gtggttgcag cccagtctat tggagaacct ggaacacagc tgaccctgag aactttccac
     8641 cagggaggta ccgcttcgac cttccagtct gaaagcgaga tcgtggcccg ttataacggt
     8701 atcctggaga tagatgagct gagaaccctc aataaggttt ccccaactgg cgaaacccag
     8761 accatcgtta ttggcagaac tgcagaaatg cgtattgttg acagtaacac acgcataaca
     8821 ctatctacct acaacatacc ttacggggcg aacctgtatt tcaaagacgg ggatgaagta
     8881 agggaaaagg ataaaatctg cgaatgggac gcctataatg cagtgaccat agccgaactt
     8941 gacgggacag ttgcttttga cagcctgatt gaaggcgtta cctaccgcga ggaagcagcc
     9001 gatgaatatt tcctccacag ggacaaggtg atcatagaaa gccgcgacag atcacgcaac
     9061 ccgaccctta agatcatgaa aaacaatgtg gaactcaagc agtacaacct gcctgtggga
     9121 gcccatatca tggttaatga aaaacaggag atcaaagc
//
LOCUS       DAOSOF010000074         9096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7600_length_9096_cov_4.474616, whole genome shotgun sequence.
ACCESSION   DAOSOF010000074 DAOSOF010000000
VERSION     DAOSOF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7600_length_9096_cov_4.474616"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLM68_05365"
     CDS             <1..523
                     /locus_tag="PLM68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT54828.1"
                     /translation="SITSVQAVYVPADDLTDPAPATTFSHLDATTVLSRKITEMGIYP
                     AVDPLESTSRIMDPAILGDKHYNTAQRVKQILQRNKELQDIISILGMEELSREDRMTV
                     MRARKVQRFLSQPFHVARQFTGIPGVIVSIDDCIKGFNMIMDGEVDHIPENAFLNVGT
                     IDDAIEKSKHLMG"
     gene            526..774
                     /locus_tag="PLM68_05370"
     CDS             526..774
                     /locus_tag="PLM68_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54829.1"
                     /translation="MNALIYLQVISTQKIIFQGNVTVARFPGKKSAFSVMKDHAPILS
                     LLEKGFITFEGPDGNGKVPVTSGFVQVRKNEVYACIDQ"
     gene            806..1201
                     /locus_tag="PLM68_05375"
     CDS             806..1201
                     /locus_tag="PLM68_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54830.1"
                     /translation="MIKKMMIVHASVILSAGSIWGLLMYLFMPQWWFPAYPIIPATFL
                     AVGIVEILLMAKSKTRHAKLYNNVLIQRIIRWGILLAVLLLLIGVYHPPKAAFIISFM
                     GLFLVYSFLSIWFHLVDIKVNKKKAQLEG"
     gene            1217..2326
                     /gene="atpB"
                     /locus_tag="PLM68_05380"
     CDS             1217..2326
                     /gene="atpB"
                     /locus_tag="PLM68_05380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT54831.1"
                     /translation="MKRRILICFLFLFLILEFPATVQAAQKERPPVDAREVILGHLKD
                     SYHWHFFDIGERHISLPLPVIVHSKERGWFVFLSSRLEESGMYKGFSIAEEGRSAGKI
                     VEKDGSGNQTRPFDISITKNVVSLFFTCILLIFLVLNLAKWYRKREEEGDPDAVPSGL
                     NGIIEWVTMSIYEGVIIKCVGKDHKRYAPYLLTAFFFIFLTNLLGLVPIFPAGANLTG
                     NIAITMSLALCTMIAINIFGNKEYWKEILWPDVPIFMKAPIPIMQIIEFFGILTKPMA
                     LMIRLFANIFAGHIIILALTSLVFLTVAKGTAINASMSAVSVLFSVFMMVLELLVAFI
                     QAYVFTMLSSIFIGLSRKEPHKTAIPAGDIKINKK"
     gene            2355..2609
                     /locus_tag="PLM68_05385"
     CDS             2355..2609
                     /locus_tag="PLM68_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT54832.1"
                     /translation="MLLMTLLQAATYSVTFTKLGAVLGAAIVVLGASFGISKIGTSAM
                     EAIARQPEAADGIRMSMIIIGALVEGVSLFAVIVCLLVAL"
     gene            2622..3122
                     /gene="atpF"
                     /locus_tag="PLM68_05390"
     CDS             2622..3122
                     /gene="atpF"
                     /locus_tag="PLM68_05390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT54833.1"
                     /translation="MDLMIPDSGLLFWMLIAFGILFFILARYGWPLITGVVERRNERI
                     AKALSDAAAARQLLATLKQQEEKILQQSRDEQARLVEQTEALRIKLTEQAREAAQEES
                     RIILEKAREQIHKEKEMVMQELRLYVASLSVEVAEKILRKQLEPDNRQIELINQLIDE
                     TIKPRA"
     gene            3127..3684
                     /gene="atpH"
                     /locus_tag="PLM68_05395"
     CDS             3127..3684
                     /gene="atpH"
                     /locus_tag="PLM68_05395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOT54834.1"
                     /translation="MNTGVIASRYATTLLQYATSCMTECDLYDMMKRISACFAEIPDL
                     EKTFSNRMLSAEERKALFLDLFPGAPEKPLKVLSDFLDLLIRQRRENIIRSVSLLYLD
                     KYRLQEGITYGVLTTVAPIDNLTAEKLKSSLDPVNPQKVELELKQDPGLIGGFSLRVG
                     MKVWDASLLARLREVRKAYGLKDIY"
     gene            3692..5266
                     /gene="atpA"
                     /locus_tag="PLM68_05400"
     CDS             3692..5266
                     /gene="atpA"
                     /locus_tag="PLM68_05400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT54835.1"
                     /translation="MPDLIKPAEVTDILRIQLEGLDSRPVYEEKGVVLQVSDGVVRIY
                     GLYNAGANELLEFDNGIRAVVMNLEEDNVGAVLLGPVSKIREGDMVRRTGTIASIPVS
                     EKMLGRVIDPLGNPLDGRGAIPGPFTEMPLERKAPGVIFRQPVSEPLQTGIKSIDAMI
                     PIGRGQRELIIGDRQTGKTAIALDTIINQRNNFLAGDPVYCIYVAIGQKGSTVATIVN
                     TLEKHGAMDYTVVVSATAGDPAALQYFAPFAGASIGEYYRDTGRHALVVYDDLSKQAV
                     SYREVSLILRRPSGREAYPGDVFYLHSRLLERSAKIIEQDEIASQMNDVPDSLKGRIK
                     GGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLDTDLFNQGYRPAINVGISVSRV
                     GGDAQLKAMKQIAGTLKIDQAQYRELESFSRFGGDMDPMTMKLLDRGQKNSQLLVQKQ
                     YNPLPVEKQIALLYCGTHGLLERVTLEKIPVFEEKFLRILETSHKETVLDTLSRGEIN
                     PQIASIIESVAEQVAG"
     gene            5270..6250
                     /gene="atpG"
                     /locus_tag="PLM68_05405"
     CDS             5270..6250
                     /gene="atpG"
                     /locus_tag="PLM68_05405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOT54836.1"
                     /translation="MESLREIRRRINSVRRTQKITEAMHRISSAKLHKAQTALGYILP
                     YEERLHYMLLTFIHEERYLYEHGFPHGMSALRQTPYALARPVNQVSIIFMASDTGLCG
                     AYNANIEKKCLEALGEYANLPRENITLYPVGIKMLEVAQKTGCKVDDRYVGLMAHPNA
                     KTCERLSRRLEIGYMRGMVDKAELIYYHFKNMSVQILQRDIFLPFQSELVKSTNPAFG
                     CEIRGKQGPIIPQEYPLAELDPEYYEGFIIEPSRQELIDALLPRVLSFQLYMAVLDAN
                     ASEHAARTIAMKAASENAQDLVRDLTLLYNKSRQQAITAEILDILSGSVR"
     gene            complement(6255..6548)
                     /locus_tag="PLM68_05410"
     CDS             complement(6255..6548)
                     /locus_tag="PLM68_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOT54837.1"
                     /translation="MDIRVESVKFDADRKLLDFIDEKVGKLDRFFDAIIGVEVTLSLV
                     PGHDNKKATLRVKVPGNELIAERTCPTFEEAVATGVDILKENLVKTKQKMRGN"
     gene            complement(6596..7456)
                     /locus_tag="PLM68_05415"
     CDS             complement(6596..7456)
                     /locus_tag="PLM68_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT54838.1"
                     /translation="MERSDFIDFLRVQKRYSIRTQSIYNHAITDFYNFMDFQKEDNPM
                     KEVSLNHLRAYTGWLLEQGLSPATVYQRLSALSSYCNYLVRKGKLPDNPLKLLIRPKK
                     GRRIPEFYPEASINNLIDRSESSPTLVSFMIHLIYATGIRRAELAGIRIEDIDFYRSL
                     LRVRGKGNKERDVPLGKGLVRDLQQFIAERNINSPWLFCRKDGQPLALTTISKMVHKE
                     LSGEPGFTGKKSPHVLRHSLATHLLNRGADLNSIKELLGHDSLTATQVYTHNSFEALK
                     KAYKKAHPRA"
     gene            complement(7485..7676)
                     /gene="rpsU"
                     /locus_tag="PLM68_05420"
     CDS             complement(7485..7676)
                     /gene="rpsU"
                     /locus_tag="PLM68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT54839.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGIVRELRSRQAFSKPSVT
                     KRDQQKKAVYKQRLQNNEE"
     gene            7786..>9096
                     /locus_tag="PLM68_05425"
     CDS             7786..>9096
                     /locus_tag="PLM68_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOT54840.1"
                     /translation="MFNEGIPSQIPPEIPLDESVNHAPVRKDILSKEEKKLALANALR
                     YFPAEQHAALAPGFARELKQYGRIYMYRYRPQKPITAGRLEDYPAKCHQAAAIMLMIN
                     NNLDKAIAQHPHELITYGGNGAVFQNWAQYHLCMHYLSQMTDEQTLVLYSGHPLGLFP
                     SSKEAPRLVITNGMVIPNYSSRNHWEKLNALGVSQYGQMTAGSFMYIGPQGIVHGTTI
                     TIMNATRMRTQETKGSLLFVSSGLGGMSGAQPKAARIAGVVGVIAEIDPLATAKRYEQ
                     GWVDEVYHDLGSLTTRILEAKRGGEAVSLAYQGNVVDLWEHLAAEGIGVELGSDQTSL
                     HNPFSGGYYPVGYTLEESRKMIAKDPGHFEEAVRLSLRRQVKAINTLATRGMYFFDYG
                     NAFLLEASRAGADIMLANGKFRYPSYVQDIMGPLFFDYGFGPFRW"
BASE COUNT         2427 a         2108 c         2263 g         2298 t
ORIGIN      
        1 ctccatcacc tctgtacagg cggtatatgt acctgccgac gacctgacgg acccggcacc
       61 ggccaccacc ttctctcacc tggacgctac cacggtgctt tcccgtaaaa ttaccgaaat
      121 ggggatctac ccggctgtgg atccgctcga atccacttcc cgtatcatgg atccggccat
      181 cctgggggat aaacattaca acacggcaca acgggttaaa cagatattgc aacgcaataa
      241 agagttgcag gatatcatat ccatcctggg aatggaagaa ctttcccggg aagaccgcat
      301 gactgtcatg cgggcacgta aggtgcaacg cttcctgtcc cagcctttcc atgttgcccg
      361 gcagtttacc ggaataccgg gagttatagt ttccatagat gattgtatta aaggctttaa
      421 tatgataatg gacggtgaag tggatcatat tccggaaaac gcattcctga atgtggggac
      481 cattgatgat gcgatagaaa aaagcaaaca tttaatgggt taacaatgaa cgcattgatc
      541 taccttcagg taatatcaac acaaaagatc atattccagg gaaatgtaac agtagccaga
      601 ttccccggaa agaaaagcgc attttcggta atgaaggacc acgcgcccat cctgtctttg
      661 ctggaaaaag gttttataac ttttgaagga cctgatggaa atgggaaggt cccggtaacc
      721 agtggttttg tgcaggtcag gaaaaacgag gtgtatgcct gcatagacca gtgaaatatt
      781 gttaatcatc taaacccggt ccgttatgat aaaaaaaatg atgatagtac acgcttcggt
      841 cattctttct gccggatcta tatggggtct tctcatgtac ctgtttatgc cccaatggtg
      901 gtttcctgcc tatcctatca tcccggcaac atttctggcg gtaggcatag tggaaatcct
      961 gttgatggca aaaagcaaga cacggcatgc gaaattatac aacaatgttt tgatacagag
     1021 aataatacgc tggggcatcc tattggcggt actgctgctt ctcattgggg tgtaccaccc
     1081 gccaaaagct gcattcatta tcagtttcat gggacttttt ctggtctaca gctttctctc
     1141 tatctggttt catctggttg acatcaaagt caacaaaaag aaagcgcaat tagagggtta
     1201 atttattgtt ttacaaatga aaaggcgtat tctcatatgt tttttgttcc tgttccttat
     1261 cctggaattc ccggcaactg tgcaggcggc acagaaagaa aggccgcctg tagatgccag
     1321 ggaagtgatc ctgggccatc tgaaagacag ttaccactgg catttctttg atattggaga
     1381 aagacacatc agccttccct tgcctgtcat tgtgcacagc aaagaacgcg gatggtttgt
     1441 attcctttct tcccgcctgg aagaatccgg gatgtacaaa ggattttcaa ttgctgaaga
     1501 aggcaggtct gcgggaaaaa ttgtggagaa ggacggaagt ggcaaccaaa ccaggccttt
     1561 tgacatctcc ataacaaaaa atgtggtttc tttgtttttt acctgcatcc tgcttatttt
     1621 cctggtgcta aacctggcaa agtggtaccg gaaacgggag gaagagggtg atcccgatgc
     1681 cgtaccttct ggtttaaatg ggattataga gtgggttacc atgagcattt acgaaggggt
     1741 gatcataaag tgtgtgggca aagatcataa acgctacgct ccctatttgc tgaccgcttt
     1801 tttcttcatt ttcctgacca acctcctggg attggtgccc atatttcctg ccggtgcaaa
     1861 ccttacaggc aacattgcca ttaccatgag cctggccttg tgcacgatga tagctattaa
     1921 tatttttggc aataaagaat actggaagga gatcctgtgg cccgatgtgc ccatatttat
     1981 gaaagcaccc atccccatca tgcagatcat tgagtttttt ggtatcctta ccaaacccat
     2041 ggccctgatg attcgtcttt ttgccaatat attcgcagga cacatcatca tactggccct
     2101 gacatccctc gtatttttga ctgttgccaa agggacggca atcaacgcct ccatgagtgc
     2161 ggtatcggtg ctcttctctg tcttcatgat ggtgctggaa ttgctggttg cctttatcca
     2221 ggcttatgtt tttaccatgc tttcctccat ctttatcgga ctatcccgga aagaaccgca
     2281 taaaacggca atccccgcag gagatattaa aataaacaag aaatgaaaat tcaaaatttc
     2341 aaaataatac tattatgtta cttatgactt tattgcaggc agcgacgtac agcgtcacat
     2401 ttacaaaact gggggctgtt ctgggcgcag ccattgtggt attgggtgca tcttttggca
     2461 tcagcaagat cgggacttcc gccatggagg ccatagctcg tcagcccgaa gcggcagacg
     2521 ggatcaggat gtccatgatc attatcggtg ccctggtgga aggggtgtcc ctttttgcag
     2581 tcatcgtgtg cctgttggtt gccctttaaa gaactccggc tatggacctg atgattcctg
     2641 attcaggatt gttgttctgg atgctgattg cattcgggat cctgtttttt atactggcac
     2701 gttatggctg gcccctgatc accggagtgg tggaacgtcg caacgaaagg attgccaaag
     2761 cattatccga tgcggcagca gcccggcaat tgctggccac cctcaagcag caggaagaaa
     2821 agatcctgca gcaatcacgc gatgaacaag ccaggctggt tgagcaaaca gaggctctca
     2881 ggattaaatt aacagaacag gcccgagagg cagcacaaga agaaagccgc atcatacttg
     2941 aaaaggccag ggagcaaatt cacaaggaaa aagagatggt gatgcaggaa ctcagactgt
     3001 atgtagcatc cctgtctgtt gaagttgccg agaaaatatt gcgcaagcag ctggaaccgg
     3061 ataacaggca aattgaactg atcaaccagc ttatagacga aaccatcaaa cccagggcat
     3121 gatgatatga acacaggagt gatagcatcc cggtatgcca ctactttgct gcagtatgcc
     3181 acatcgtgca tgacagaatg cgatctgtat gatatgatga aacgcatatc ggcttgtttt
     3241 gccgaaattc cggatctgga aaagactttc tcgaacagga tgttatccgc ggaagaaagg
     3301 aaggcccttt ttctggactt gtttcccggg gcaccggaga aacctctgaa ggtgctatcg
     3361 gatttcctgg atttgctgat acgtcagcgg agggaaaata tcatccgctc ggtcagcctt
     3421 ctgtatttgg ataaataccg cctgcaggaa ggaattactt atggtgtgct gaccacggta
     3481 gctcccatag acaatcttac agcggaaaaa ctcaaaagct cactggatcc tgtcaaccca
     3541 cagaaggttg aactggaatt gaaacaggat cccggtctga ttggagggtt ctcactaagg
     3601 gtggggatga aggtatggga cgcttcgctg cttgcccgtt tacgggaagt aaggaaggcg
     3661 tatggattaa aggatattta ctaggtatgc tatgccagat ttgataaaac ccgctgaagt
     3721 caccgatata ctgcggatac agctggaagg acttgactcc cggccggttt atgaagaaaa
     3781 gggagttgtg ctacaggtga gtgacggggt tgtccgcata tacggactgt acaacgcagg
     3841 agccaatgaa ttgcttgaat tcgacaatgg tatcagggcc gtggtcatga acctggagga
     3901 agataatgtg ggggcagtcc tgcttggacc tgtcagcaag atccgggagg gtgacatggt
     3961 gcgaaggaca gggaccatag cctcgattcc cgtgagtgag aagatgttgg gcagggtgat
     4021 tgatcccctg ggtaatcccc tggacggaag gggagccatt cccggacctt tcactgaaat
     4081 gcccctggaa cgaaaagccc cgggtgtgat cttccgtcag cccgtatccg aacccctcca
     4141 gacagggata aaatcaatag atgccatgat ccccataggc agggggcaaa gggaactgat
     4201 cataggcgac cggcaaacgg gaaaaacagc tattgccctg gacacgatca tcaaccagcg
     4261 caacaacttc ctggccgggg accctgtcta ttgcatctac gtggccatag ggcaaaaagg
     4321 atccacggta gccactatag tcaataccct ggaaaaacac ggtgccatgg attataccgt
     4381 ggtggtttcg gcaaccgccg gagatcccgc cgcgttgcaa tattttgctc cttttgcagg
     4441 ggcatccata ggagagtatt accgcgatac aggtcgccat gccttggtcg tatatgatga
     4501 tctttccaaa caggcagttt cttaccggga ggtatcactt atcctgcgca gaccatcggg
     4561 aagggaagcc tatccgggag acgtgtttta tttgcactcc aggttgctgg agcgttctgc
     4621 aaagatcatt gaacaggatg aaatcgcctc acagatgaat gatgtaccgg actcactgaa
     4681 aggcaggatc aaagggggag gatcccttac agcacttcct gtcatagaaa cgcaggcagg
     4741 tgatgtctcg gcctatatac ctacaaatgt gatatccatt acagatggac agatcttttt
     4801 ggacacggat cttttcaacc agggatacag gccggccata aacgtgggaa tttctgtttc
     4861 aagggtggga ggagatgccc agctcaaggc tatgaaacag attgcgggca cgttgaagat
     4921 cgatcaggct cagtaccggg aactggaatc attctcacgt tttgggggag atatggatcc
     4981 catgaccatg aagctcctgg acaggggaca gaagaattca caactcctgg tacaaaaaca
     5041 atacaatccc cttccggtgg aaaaacaaat tgcattgcta tattgcggga cacatgggtt
     5101 gctggaaagg gtcaccctgg aaaaaatacc ggttttcgaa gaaaaattcc tgagaattct
     5161 ggaaaccagt cataaagaaa cggtgcttga tacgctgagc aggggagaga tcaatccgca
     5221 gatagcatca atcattgaaa gcgtggcaga gcaggtagcc ggataaacca tggaatcttt
     5281 acgtgaaata agaagaagga tcaattctgt ccgcaggacg cagaagatca cagaagccat
     5341 gcataggatc tcatctgcaa aacttcacaa ggcacaaaca gcccttgggt atattctgcc
     5401 ttatgaagaa aggttgcatt acatgcttct gacctttatt cacgaagagc gttacttgta
     5461 tgagcacggt tttcctcatg gcatgagtgc tctgcgacag actccttatg cattggcccg
     5521 tcccgtaaac caggtatcca taattttcat ggcttcggat acggggcttt gcggtgctta
     5581 caatgccaat attgaaaaaa aatgccttga agcgctgggt gaatacgcca acctgccccg
     5641 cgaaaacatc accctctacc ctgtagggat aaaaatgctg gaggtggcac agaaaacggg
     5701 gtgtaaggtg gatgaccgct atgtgggcct gatggcacat cccaatgcca aaacatgtga
     5761 aaggttatcc cgaaggctgg agatcggtta catgaggggc atggtggata aggcagagct
     5821 tatctattat catttcaaga acatgtctgt ccagatcctt cagagagaca tctttctgcc
     5881 atttcagtca gaactggtaa aaagcacaaa tcctgctttc ggatgcgaga taaggggtaa
     5941 gcaaggcccc atcattccac aggaataccc gctggcagaa cttgaccctg aatactatga
     6001 aggattcatc atagaacctt cacgccagga actcatagat gcactgttac ctcgggtact
     6061 gagtttccag ttatacatgg ctgtgctgga tgccaatgcg tcggaacatg ctgcaaggac
     6121 aattgccatg aaagcagcat ccgagaacgc gcaggatctt gtccgcgatc tgacactact
     6181 ctataacaaa agccgtcagc aggccattac agcagaaatt ctcgatattt tatcgggttc
     6241 ggtaaggtaa cagatcagtt gcctcgcatt ttctgttttg ttttcaccag gttttctttc
     6301 aatatatcca cacctgtagc gacagcttct tcaaaggttg gacaagttcg ttccgcaatg
     6361 agttcatttc cgggaacctt gacacgaagg gtggcttttt tattatcatg accgggtacc
     6421 agtgaaagag tcacctcaac tccaatgatg gcatcaaaga aacgatccag tttccccact
     6481 ttttcgtcaa tgaaatcgag aagtttcctg tcggcatcaa attttacgga ttcaactcta
     6541 atgtccatga tatacctcca ttttatattg ttaataacta cgccccgggg agctttcatg
     6601 cccttgggtg cgctttttta taggcttttt ttagtgcttc aaatgaattg tgtgtgtaaa
     6661 cctgcgtggc tgtgagcgaa tcgtggccaa gcaactcctt gatgctgttc aggtctgccc
     6721 cgcggttcaa caagtgggta gccagggagt gacgcagcac gtgggggctt tttttcccgg
     6781 taaatccggg ttcgcctgac aattctttgt gtaccatttt gctgattgtt gtaagggcca
     6841 gtggttgccc gtctttacgg cagaacaacc agggtgaatt gatgttcctt tcagcaatga
     6901 attgctgcaa atcccggacc aaacctttgc ccaggggaac gtcccgctct ttgttgcctt
     6961 tgcccctcac acgaagcaaa gaccggtaaa agtcaatgtc ttcaatacgt attcccgcca
     7021 gctctgcccg gcggatcccc gtggcataaa tcaaatgtat catgaacgac accaaagttg
     7081 gggatgattc gctcctgtct ataaggttgt tgatgcttgc ttcgggatag aactcgggaa
     7141 tacgccttcc ttttttcggg cgtatgagca gtttcagggg attgtccggg agcttccctt
     7201 tcctgaccag gtagttgcaa taggaactca atgcagagag tctctggtat acggtggcag
     7261 gactcagacc ctgttccagg agccagcctg tgtatgccct cagatggttc agcgacacct
     7321 ccttcatagg attgtcttcc ttctggaagt ccatgaagtt gtaaaaatct gtaatggcgt
     7381 gattgtaaat tgattgagtt cttattgaat agcgcttttg aaccctcagg aagtctatga
     7441 aatcacttct ttccatcgta tacaaatata caaaaaaacg aggattattc ctcgttattc
     7501 tgcaaccttt gtttatatac ggctttcttt tgctgatccc ttttggtaac ggagggtttg
     7561 gaaaaggcct ggcggcttct gagctcacgc acaatccctg tcttttcaaa tttccttttg
     7621 aatttcttta atgcgcgttc tatgttttcg ccttccttaa caggtacaat aatcatatta
     7681 gcagacacct ccttttgtta cggggtgcaa aagtagacaa aaaaatggaa aatcaaaggg
     7741 taaactgtat atttgtaagt taaatgctga aaattccatt cgattatgtt caatgaaggc
     7801 attccttcac agattccacc ggaaattccc ctggatgaaa gtgtaaacca tgcgccggta
     7861 agaaaagaca tcctttcaaa ggaagaaaaa aagctggccc ttgcaaacgc actccgctat
     7921 tttcctgccg agcagcatgc agcactcgca cccggatttg cccgtgaact gaagcagtac
     7981 ggcaggatct acatgtaccg ctaccgtcct caaaagccca ttactgcagg caggctggaa
     8041 gattatcctg ccaagtgcca tcaggcagcg gccatcatgc tcatgataaa caacaatctg
     8101 gataaggcca ttgcccaaca cccgcacgaa ttgatcactt acgggggaaa cggggcggtt
     8161 tttcagaact gggcccagta ccatctgtgt atgcattacc tttcacagat gacagatgag
     8221 cagacccttg tgctgtattc gggccatccg ctgggacttt tcccttcatc aaaagaggca
     8281 ccgcgcctgg tgatcaccaa cgggatggtc attcccaatt attcctcgag aaatcactgg
     8341 gagaaactga atgcactggg cgtttcacaa tacggacaga tgactgccgg ttccttcatg
     8401 tatatcgggc cgcagggaat cgttcatggc acgacgataa ccatcatgaa tgccacaagg
     8461 atgcgcacac aggagaccaa gggctcactg ctgtttgtat ccagcggcct gggagggatg
     8521 tctggtgcac agccaaaagc cgccaggata gcaggcgttg tgggtgtgat tgccgagatt
     8581 gatcccctgg caacggccaa aagatatgag cagggttggg tagacgaagt gtaccacgac
     8641 ctcggatcac tgaccacccg tatcctggaa gccaaaaggg gaggagaggc agtttcactc
     8701 gcctaccagg ggaacgtggt ggacctgtgg gaacatctcg ccgcagaagg gattggggtt
     8761 gagctgggat ctgaccagac aagcctgcac aaccctttct cgggaggata ttacccggtt
     8821 ggatataccc tggaggaatc cagaaaaatg attgcaaagg accccggtca ttttgaagaa
     8881 gctgtccgcc tgagcctcag acgacaggtc aaggccataa acacgttggc tacacggggc
     8941 atgtatttct ttgattacgg taatgcattc ctgctggaag catcacgcgc aggagcggat
     9001 atcatgctgg caaacgggaa attccgttac ccttcctatg tgcaggacat catgggaccc
     9061 ttgttctttg attacggctt tggtcccttc cgctgg
//
LOCUS       DAOSOF010000075         9054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7647_length_9054_cov_5.615291, whole genome shotgun sequence.
ACCESSION   DAOSOF010000075 DAOSOF010000000
VERSION     DAOSOF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_9054_cov_5.615291"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PLM68_05430"
     CDS             complement(<1..1644)
                     /locus_tag="PLM68_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT54841.1"
                     /translation="MLLVLAACNQRSRTVIQEWIPYDEAEEIAANADHESVRMRFRLI
                     QSQVLDKNELWKVVEPQLKNFTEEAYERLKPLILEQDIPTLQSHIRASELTYEQLTQW
                     YLYRIVKFENDRGKMLNAIVAINPDAVKEARAKDKALRKFLTRNKGIEGQEAPALHLI
                     FGMPILVKDNIGVEGMPTSAGAHVLKDNFTKDAAIIANIKKCGGIILGKTNLSEWANY
                     LFNGGPNGFSAVGGQTLSAYGRRIFDTGGSSSGSGVAIAAGYAAACIGTETSGSILSP
                     SGKSSVVGLKPTVGLLDRSGIVPLAGTLDTPGPMAGTVVDNAILISAMAGDSLMKDDW
                     SAGLLKSLDPAILSEESPQEGDKGLTSERETGFPEGIRFGVNKKLLENPLYKEQAEIL
                     TSLGGIAIEFEPIEIDLSGFGTLLSGDMKIDLPAYLEQYAPDYLTIRTIGEIVAYNSL
                     DSLVRIPYGQAIFEEMAALEITPEALGQLRERLQQEGKRYFETPMDAYRLDVILSVDN
                     LEAAYAAAANFPCLIVPMGYTEKWGPTGLTFIARSFEEHK"
     gene            2357..2743
                     /locus_tag="PLM68_05435"
     CDS             2357..2743
                     /locus_tag="PLM68_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT54842.1"
                     /translation="MVNVFIGSIIRQKVRERKLTVSEFARSINRSRTTVYDIFRRKSI
                     DVDLLLTISQALDYDFIGEIYLTKRDNIAKKCFLAIEIDPSELPCDSKEIAERDKLEV
                     RVLLKEKLKSIIIKEKEREQKRANCE"
     gene            3001..5220
                     /locus_tag="PLM68_05440"
     CDS             3001..5220
                     /locus_tag="PLM68_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54843.1"
                     /translation="MYAQQQLVIDSLLRIVESTPSDSVRICNLGEVAWLYYNVKPAMA
                     LEYAHQGLKLSEKTGINQGLSVILNTLGAAHTTMGNFDSALVYYERSMDFAEKMKDTL
                     KIAAVKDNISSIYLYKRNYSTALKLREESNKIYKKAEKTLLLAHGYVWIGNIYLSQDK
                     FDTALQYYLEAKRIFEKENDLGEAYALINIGTVYRRMKHYEQAIDNLERSKELFKEAG
                     DLNNVGTSLYRISTIYFDIKDREKYLETLFEAEEVLTSVGNEYFLSLIYSRLSDEYKL
                     IEDYERALFYLNKTLVYGEKSGAQDVTTIARHGIATIYYAKQEYTTALDYLRKAEQTA
                     VNLKVHLPQIYLDYVRVYSRLNEPDSVEKYVYAYSSLTDSLFSDKTIQAVADIQAKYD
                     SEKKEKEIAILNLENESIEHELEQLNQKNQINQLNYRQSLIENENYRQSLLLAETERE
                     NQRSEIEVMTLNEEIHKQSMEHEKRTKQLMTLGFVIVFLALIVTTIFIFLWYTNKKKK
                     EKALLQQRAAELSKQLMEINMKALNFQLNPHFIFNCVQTVEFLLKESKTEESMESLRK
                     FSNLTRLMLESMSKKEIPLEQELEIIRFYMDLEKTRYINSFSYSIEVDRDLDTEATMV
                     PPLILQPFVENSIKHGFAGKEKGNEIKISIRPIQNNLLCEITDNGLGFSATQQAKTPV
                     SGYKKESLGLKLTRERLQVINKMKNASSSFTIEDILTEDNKTKGTRVSLYLPNTSAA"
     gene            5217..5981
                     /locus_tag="PLM68_05445"
     CDS             5217..5981
                     /locus_tag="PLM68_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT54844.1"
                     /translation="MIKTIIVEDDVMHARSLTEMIEEHFKNIRILSVCNNLPDAVQKI
                     QELKPDLVFLDVELGPYNGFDLLAMFNRRDFEVIFTTAYQKYAVMALKMSALDYIEKP
                     VQIDQLREALSRYKSRSGEKKMQNLLRNFQASQEEQKIALFDQGSFLFIMVKDIIWLS
                     SENAYTKIRFRDGEAVNKVLMSKSIAYYQDMLEGKGLFFRVHNQHLVNIRFIKRLVLD
                     STCDIIMDDNERSVVPVARSRREQFIDYLHDNGVMF"
     gene            complement(5992..7035)
                     /locus_tag="PLM68_05450"
     CDS             complement(5992..7035)
                     /locus_tag="PLM68_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT54845.1"
                     /translation="MKKHWIFLVLMLLPAWFLSAQEVDHQSLGLSKQIDDLMWYYKVG
                     DVAYIDKVYIPTVPRWKEDNPAGQGAGNPLKIWSYVFVPRDIDPSGKYPLIVLPHGGV
                     HADFTTYHTHIVRELIAQGYIIIATEYRGSTGYGQTLYEDIDYGGLENEDVWYGRNYM
                     VENYDIVDPSRVGIMGWSHGGMITLMNLFEHPKDYKAGFAGVPVSDLIARLGYKKPGY
                     MALFSAPYHIGKTPEEDVAEYRRRSPVWQAARLQTPLLIHTNTNDEDVNSLEVEHLIN
                     ALKAHGKSFEYKIFENHPGGHSFDRMDTPQASAIRLDIYKFLARYLNPPKPFKNVRQL
                     RDAAYHPWAANKD"
     gene            complement(7038..>9054)
                     /locus_tag="PLM68_05455"
     CDS             complement(7038..>9054)
                     /locus_tag="PLM68_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT54846.1"
                     /translation="PDSRGLYFTTEKRELWHAVPATGTARCVLQTPYSAIRSYDISPD
                     GKWITYSTQAENRMDVVYLYSVDDNISYPVTSPWYDAQSGVFSDDGKYLFFTSARDFS
                     ASYSRVEWNASYNVSSYVFVLPLAKGTPDPTALKAVEYKPSEKSDTPPAPPAAPKGRK
                     NDKAEPAAEKQVNVTVDLDGIVERASALPLDPGMYWLLAAVDGDLYFSTRRDIKKISL
                     SDLKESPVVTGMPLAYAPKGKKMLLRDGEDLFIIGAPSWKKDKKVMLQDVRVFTDYPA
                     EWNQIFNETWRVYRDHFYLPTMHGRDWEGIREKYEVLLPYVKHRHDLTYLIGEMISEL
                     NVGHCYVTSPSDTPAPERVKVGLLGARFGKDASGAFRVIRIYEGENWDKSLRSPFREG
                     GVDIAQGDYIMEVDGVDCSSMKVIYEALIGKVDKTVALTVNSSPSRTGARTVYVRPIA
                     DEASLMYYEWVQENINKVNRASNGQIGYIHIPDMGIAGLDMFTKLFYTQLDKKALIID
                     DRMNGGGNVSPMILERLAREAYRMSMYRHGVNLPVPNESHSGPKVCLIDKYSMSDGDL
                     FPYGFRKMGLGKLIGTRTWGGIVGISGSKPYLDGQDVRTPFFTSYSTDGEWIIEGHGV
                     EPDIEVDINPFEDYLGNDAQLNKAIEVLLEELKSFEPLPGTPPDRKM"
BASE COUNT         2532 a         2058 c         2109 g         2355 t
ORIGIN      
        1 cttgtgttct tcaaaggacc tggcaataaa ggtgaggccc gtgggacccc acttctctgt
       61 atagcccatg ggtacgatca ggcacgggaa attggctgct gccgcatagg cggcctccag
      121 gttgtctaca gacaaaatga catccagccg gtaagcgtcc atgggggttt caaaatagcg
      181 ctttccctcc tgctgtagtc tttcccttaa ctgccccaat gcttccggtg taatctcaag
      241 cgcggccatt tcttcaaaga ttgcctgccc gtaagggatc cttaccaatg agtccaggct
      301 gttatatgcg acgatttctc ctatggtgcg gatagtcagg tagtcaggag cgtattgttc
      361 caggtaggca ggcaggtcaa ttttcatatc tccgctcaac aacgtgccaa acccgctgag
      421 gtcaatttca atgggttcaa attcaatggc aatgcccccg agcgaagtga ggatttcggc
      481 ctgctcctta taaagcggat tttccagcaa ttttttattt accccaaacc gaatcccttc
      541 cggaaacccc gtttcccgtt ccgatgtcag ccctttatcc ccttcctgtg gactttcttc
      601 gcttaaaatc gccggatcca ggctctttag gagccctgca gaccaatcat ctttcataag
      661 gctatcgcca gccattgccg agatgaggat ggcgttgtca acgactgtgc ccgccatggg
      721 cccgggagtg tccaatgtgc cggcaagtgg aacgatccca ctccggtcca gcaggcccac
      781 cgtgggttta agccccacaa cggaactctt tcccgaggga gaaagaattg agcccgaggt
      841 ttccgttccg atgcaggcag cagcatatcc ggccgctata gcaacgccgc tgccggaact
      901 ggaaccgccg gtatcaaaaa tcctgcgtcc gtaggcactg agcgtttggc caccgacggc
      961 gctgaatcca ttaggacctc cgttaaacag gtaattggcc cattcgctca ggtttgtctt
     1021 gcccaggatg atacccccgc attttttgat atttgcaatg atggcggcat ctttcgtaaa
     1081 gttgtctttc aacacatgtg ccccggccga ggtgggcatc ccctcaaccc cgatgttgtc
     1141 cttaaccagg ataggcatcc caaagatcag atgcagggca ggggcctctt gtccctcaat
     1201 ccctttatta cgggtaagga atttgcgcag agccttgtct ttggcacggg cctccttcac
     1261 cgcgtcggga tttatggcaa caatggcgtt cagcatcttg ccccggtcgt tctcgaactt
     1321 cacgatcctg tataggtacc attgtgtgag ttgctcatac gtaagctcac ttgcgcggat
     1381 atgcgactga agggtaggaa tgtcctgctc caagatcaat ggtttcagcc tctcataggc
     1441 ttcttcagta aagtttttga gctggggttc cactactttc cacaattcat ttttatccag
     1501 cacctgggac tggataagcc tgaagcgcat tctcacagat tcatgatctg cattggcggc
     1561 tatttcttct gcctcgtcat agggtatcca ttcctggatc actgtccggg acctttgatt
     1621 gcatgcggca aggactaaga gcataagcaa agggatatga aaagttctca tgaggttttt
     1681 ttagttaatt ttctttcgtt atgacaatca tataaatata acagaccaac cataccaatc
     1741 gcacgcaatg cggcatataa taccttatac aaaacaggtc cgtcattaaa gaataaatca
     1801 gaagtaagga atccggaata aaaaaccaaa aatacaaagt gataccttat ttttttcatg
     1861 acagataagt atttgaaaat aaaggattcc caaatataaa gaaaacatat caaagttcat
     1921 aataacctgt agttgagcca gagccgaagc caccagaagg taaggaaagc ctcggcataa
     1981 agaaaaggtt tcccggcctt gtggtggtgg cagcgaccgc tggcggaaaa cgccagcata
     2041 acctgcctac cagggaggaa aatactgaaa acggttgttt ttctaccgaa ttcaaactca
     2101 aaccgaccga attcaaagtc atatgaacaa atctcaatat gaatccttat atttatatgc
     2161 gcaaaaaacc ggacaacgtg aacctgacct gcccctatga ccttaacctg acaagacaac
     2221 gctaaagtaa ccggacaatg agaaaatagc cgggcaaccc ggtgtaaccg ggaacccggt
     2281 tttccaaaag atgttcactc gggagaacat caggaataac cgaaaatgcg gttttaggcg
     2341 cactagatat gacatcatgg taaatgtatt tataggaagt atcatcaggc aaaaagtccg
     2401 tgaacgaaag ctcacggtct ctgaatttgc caggagcatt aaccgttcca gaactaccgt
     2461 gtatgatatt ttcagaagaa aaagtattga tgttgacctg ttgctgacca tatcccaggc
     2521 attggattat gattttatag gagagatcta ccttaccaaa agagataata tagcaaaaaa
     2581 atgctttctg gccatagaaa ttgacccttc tgaattgcct tgtgattcaa aagaaatagc
     2641 agaaagagat aagcttgaag tcagagtact gttgaaagaa aagctcaagt caataattat
     2701 aaaggaaaaa gaaagagagc aaaaaagagc taactgcgag taaatccgga catttgtgtc
     2761 taataatccg acgctacaga gtcttgtttt atttccggat ttttcccaat tttagaagtt
     2821 catatttttg agaaaagcaa ccgcacaatg atgcgggtgt tttatagtta tttatacgtg
     2881 tgcctataag taacctataa tatgaaacca ccaattgttc cggaaaagta ctccctgatg
     2941 agaaaaactc ttttttttac tatctgtatt ttattgtcac ttgttaaggg gtcaatacaa
     3001 atgtatgccc aacagcagct tgtcatagat tctctgctcc ggatagttga aagtacacct
     3061 tcagattctg tcagaatctg caacctgggt gaagtagcct ggttatacta taatgtaaaa
     3121 ccggcaatgg ccctggaata tgctcatcag ggattgaaac tgtctgaaaa aaccggtatc
     3181 aatcagggtc ttagcgtgat tttgaatacc ttaggtgcgg cccacactac tatgggtaat
     3241 tttgattcgg cgcttgttta ttacgaaaga agtatggact ttgcagaaaa aatgaaagac
     3301 accttaaaaa ttgccgctgt caaagacaac atatccagta tttatctgta caaaagaaac
     3361 tacagtacag cgctcaaact aagagaagaa tccaataaga tctacaaaaa agcagaaaaa
     3421 actttgttgc ttgcccatgg ctatgtttgg attggtaata tttatttatc ccaggataaa
     3481 tttgatacgg cattacaata ttatctggaa gcaaaaagaa tttttgaaaa ggaaaatgat
     3541 cttggagagg cttatgcatt aataaacata ggaacggtat acaggagaat gaaacattat
     3601 gagcaggcaa ttgataattt agagaggtcg aaagagttat tcaaagaggc aggagattta
     3661 aataatgtgg gcacctcgtt atataggatt tccactattt attttgatat aaaagacagg
     3721 gaaaagtacc ttgaaacatt atttgaggcg gaggaggtat taacaagtgt ggggaatgaa
     3781 tattttttaa gtttaattta ttcccgttta tcggatgagt ataaacttat tgaagattat
     3841 gagagagccc tgttctattt gaataaaaca cttgtctatg gagaaaagtc aggagctcag
     3901 gatgtgacta caattgcacg gcacggcata gcaactattt attatgccaa gcaagaatat
     3961 accacagccc tggattatct cagaaaagct gaacaaactg cagtaaatct gaaagttcat
     4021 cttccacaaa tttatcttga ctatgttcgt gtatacagca gactgaacga acctgacagt
     4081 gtggaaaaat atgtttatgc ttacagttct ctgactgatt cactgtttag cgacaaaacc
     4141 attcaggcgg ttgctgatat ccaggcaaaa tatgattcag agaaaaaaga aaaggagatt
     4201 gccattctga acctggaaaa tgaaagcatt gagcatgagc ttgaacagct taaccagaaa
     4261 aaccagataa accaattaaa ttacaggcag tcactgatag aaaatgaaaa ctacagacaa
     4321 agcctgctgc tagcagaaac agaaagagaa aatcaacgaa gtgagattga agttatgacc
     4381 ctgaatgaag aaatacacaa acagtcaatg gagcatgaaa agagaaccaa gcaattgatg
     4441 acattgggtt ttgtcattgt ttttcttgcc ctgattgtta ctacaatatt tatattcctc
     4501 tggtacacta ataaaaagaa aaaagagaaa gcccttttgc aacagagggc tgccgagtta
     4561 agcaagcagc ttatggaaat caatatgaag gcgctcaact tccagctgaa tccccacttc
     4621 attttcaatt gcgtgcaaac ggtggaattc ctgctcaaag agtcaaaaac agaagaatcc
     4681 atggaaagtc tgagaaagtt ttccaacctg acccggctca tgctggaaag catgtccaaa
     4741 aaggaaatac ccctggagca ggaactggaa ataatccggt tttatatgga tttggaaaaa
     4801 acacgctaca tcaactcctt ctcctattcc atagaagtgg atagagacct tgatacggaa
     4861 gccaccatgg ttcccccgtt gattttacaa ccctttgtcg agaattctat aaaacacggc
     4921 tttgctggta aggagaaggg aaatgaaata aaaatatcca tacgacccat acagaataat
     4981 cttttgtgcg aaatcaccga taacggattg ggattctcgg ctacccaaca ggcaaagaca
     5041 ccggtatcgg gttataaaaa agaatccctg ggcctgaaat tgacccggga gcggctacag
     5101 gtgatcaaca agatgaaaaa tgcaagttct tctttcacca tagaggatat actgacagaa
     5161 gacaacaaaa ccaaaggcac cagggtttcc ttatatttac ctaatacatc agcggcatga
     5221 taaaaacgat tattgtagaa gatgacgtaa tgcacgccag gtctctgacc gagatgattg
     5281 aggaacattt taaaaacatc cggatcttgt ctgtgtgcaa taatttaccc gatgcggtac
     5341 aaaagattca ggaactgaag ccggacctgg tttttcttga cgtggagctg ggcccgtaca
     5401 acggatttga tttgctggcc atgttcaacc gcagggattt tgaggttatc tttaccactg
     5461 cctatcagaa atatgcggtc atggccctta agatgtctgc gctcgattac attgaaaaac
     5521 ccgtgcaaat tgaccagtta agagaggctc tgtcccgcta taagtccagg tcgggagaaa
     5581 aaaagatgca aaatcttctg cgcaattttc aagcgagcca ggaggaacaa aagattgccc
     5641 tctttgacca gggcagtttc ctttttatca tggtaaagga tatcatatgg ctaagttcag
     5701 aaaatgcata cacgaagata cgtttccggg atggtgaggc agtaaacaaa gtgctcatgt
     5761 ccaagagtat agcatattat caggatatgc tggaggggaa aggacttttt ttccgggtac
     5821 ataaccagca cctggtcaac atcagattca tcaagcgcct ggtgctagac agcacctgtg
     5881 acataatcat ggacgataac gaacggtctg ttgtgccggt ggcacgttcc cgccgggaac
     5941 agtttatcga ctatctgcac gataacggcg tcatgttctg aaatccgtgc ctcagtcctt
     6001 attggcagcc caggggtggt aagccgcatc ccttagctgg cggacatttt tgaaaggttt
     6061 gggcggatta aggtacctgg ccaggaactt atagatatcc aggcggatgg ccgaggcttg
     6121 tggtgtgtcc atccggtcaa aggagtgccc tccggggtgg ttttcaaaaa tcttgtattc
     6181 aaaggatttt ccgtgggcct tcagtgcatt gatcaaatgt tctacctcca gggagtttac
     6241 gtcctcgtcg ttggtgttgg tgtggatgag caacggtgtc tgtaatctgg cagcctgcca
     6301 tacaggggag cggcggcggt attcggcaac atcttcctcg ggtgttttcc caatgtggta
     6361 tggggccgag aataaagcca tatagccagg ctttttgtaa ccaaggcggg ctatcagatc
     6421 gctgacggga actcctgcga atccggcttt gtaatctttg ggatgctcaa aaagattcat
     6481 cagggtgatc atcccaccat ggctccagcc catgatgcca acgcgagagg ggtcgacaat
     6541 gtcataattt tcaaccatat aattacgtcc gtaccatacg tcttcgtttt ccagccctcc
     6601 atagtctatg tcttcataca aggtttgtcc ataaccggta ctgcccctgt actcggttgc
     6661 aatgataata tatccctggg caatcaattc ccggacaata tgggtatggt aggtggtaaa
     6721 atctgcatgc accccgccgt gtggcaggac aatgagcggg tatttcccgg aaggatcaat
     6781 atcccttggg acaaagacat aggaccagat cttgagcgga ttacccgcac cctgtcccgc
     6841 agggttgtct tctttccaac gcggtaccgt aggaatgtac actttgtcta tgtaagccac
     6901 atcgccaact ttgtagtacc acataaggtc gtcaatctgt tttgacaggc caagtgactg
     6961 gtggtccact tcctgcgcgg acaaaaacca cgcaggaagc agcatcagca ctaaaaatat
     7021 ccagtgtttt ttcatgatta catttttctg tcagggggtg ttccgggcag cggctcaaag
     7081 gatttgagtt cctccaggag gacttcaatg gctttgttca gctgtgcgtc gttccccagg
     7141 taatcttcaa aaggattgat atctacttca atatcgggct ccaccccgtg tccttctatg
     7201 atccactccc cgtctgttga atacgaagtg aagaaaggtg tgcgcacatc ctgcccgtcc
     7261 aggtagggct tggatcccga gatgcccacg atgcctcccc atgtcctggt ccctatgagt
     7321 tttccaaggc ccatcttcct gaaaccatat gggaaaagat ctccgtctga catggaatat
     7381 ttatctatca ggcatacttt ggggccggaa tggctttcat tgggaaccgg caggttcacc
     7441 ccgtgacggt acatgctcat tcggtaggct tcgcgtgcaa ggcgctctag gatcatgggg
     7501 gacacatttc cacctccgtt catgcggtca tcaatgatga gtgccttttt gtccagctgg
     7561 gtgtaaaata atttggtgaa catatcgagt ccagctatac ccatgtcggg aatgtggatg
     7621 tacccgatct gaccgttgct cgccctgttg accttattga tgttttcctg tacccattcg
     7681 taatacatca aagaggcttc atcggcaatg ggcctgacat aaacagtgcg tgcccctgtt
     7741 ctggaaggtg agctgttcac tgtcagggca actgttttgt ccaccttccc tatgagggct
     7801 tcatagatca ctttcatgga agagcagtcc accccgtcaa cttccatgat ataatctcct
     7861 tgggcaatgt ccaccccgcc ttcacggaag ggggaacgaa gtgatttgtc ccagttttct
     7921 ccttcataga tcctgatgac acgaaatgcc ccggaagcat ctttcccgaa ccgggcaccc
     7981 agcaagccca ccttcacccg ttccggtgca ggtgtgtccg aaggcgaggt tacgtaacag
     8041 tggcccacgt tcaattcaga gatcatttcc ccgatcaagt aagtcaggtc gtggcgatgt
     8101 ttcacgtatg gcagaaggac ctcgtatttt tcacgaattc cttcccagtc ccttccgtgc
     8161 atggtgggca ggtaaaaatg gtcccggtaa acccgccagg tttcgttgaa tatttggttc
     8221 cactctgcag gataatctgt aaaaacacgg acatcttgca gcatgacttt tttatccttc
     8281 ttccaggagg gagctcctat gatgaaaaga tcctccccgt cccggagcag catttttttg
     8341 cctttcggag cgtaggccag cggcattcct gtgacaaccg ggctttcttt cagatcgctg
     8401 agcgaaattt tcttgatgtc tcttcttgtg gagaaataga ggtccccgtc cactgccgcc
     8461 agcagccagt acatccccgg gtccaggggc agggccgagg ctctttccac aataccgtcc
     8521 aagtctacgg taacgttgac ttgtttttcg gcagcgggtt cggctttgtc gtttttacga
     8581 ccttttggcg cggcaggagg agcaggaggc gtatcgctct tttccgaggg tttgtattcc
     8641 acggctttca gggcggtggg atcgggggtg cctttggcca gcgggagcac aaatacataa
     8701 gaagacacat tataggatgc attccattct acccgggagt aactggcact gaagtcacgc
     8761 gccgatgtga agaacaggta tttgccgtca tcggagaaca caccggactg ggcatcatac
     8821 caggggctgg ttaccgggta ggagatattg tcatccacac tgtacaggta taccacgtcc
     8881 atacggtttt ctgcctgtgt agagtatgtg atccacttgc catcggggga aatatcataa
     8941 ctgcggatgg cgctgtaggg agtctgcaac acgcagcggg cagtgcccgt agcgggaacg
     9001 gcatgccata attccctttt ttccgtagtg aagtacaatc cccgggagtc ggga
//
LOCUS       DAOSOF010000076         8917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7812_length_8917_cov_4.324983, whole genome shotgun sequence.
ACCESSION   DAOSOF010000076 DAOSOF010000000
VERSION     DAOSOF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7812_length_8917_cov_4.324983"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1860
                     /locus_tag="PLM68_05460"
     CDS             394..1860
                     /locus_tag="PLM68_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOT54847.1"
                     /translation="MKSFSKTATAVLMFLFMVACNVQDNPQDRQASPQSIQFTGRTEN
                     PATRTTLVNGIETAWESGSDRIGIFSLQARVSEETEPPAHNVAFSALESAKTSAFSGT
                     MLWGTPAAEHVFHAYYPYQSLDEPAMDAVPVSLPAEQIQPGANDSGHLSALDFMVSTP
                     VAVYAPESPGGASVNVDLTFNHVFTLMEYRITGSGQALTDIGLAGAGALSFSSGTMDL
                     DQEPGADPYTITKSGTNTYVLAHLSASATLSPTPISVYMMVLPGEQTADMSIRVKTGW
                     TWKTLTKTPPEGGFQRGSKYVTELDLDDAAFNDDEYVYIPDDNFRAYCLDNFDSDDDG
                     KITFAEAESGALTVMNVSSRSIMSLEGLQYFTHVNTLQTVGNSLTYLDVGNNLEFQTI
                     NCNSNNLTTLKAGGFTNLKILYCSYNQLSTLDLSENPDLEILTCKDNLFTSLDVSNNP
                     VLDYLDCRDNPDLQTIYVAAGQTISDLYKDDHTVIVTR"
     gene            1903..2523
                     /locus_tag="PLM68_05465"
                     /pseudo
     CDS             1903..2523
                     /locus_tag="PLM68_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
     gene            complement(2541..2933)
                     /locus_tag="PLM68_05470"
     CDS             complement(2541..2933)
                     /locus_tag="PLM68_05470"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT54848.1"
                     /translation="MTSIKTDKECPFCSIAEDREVICESAGALAIPDHYPISPGHTLI
                     IPRKHIPDYFELTGAEQQELWQLVNRCKAILTERYHPDGFNVGINVGPAAGQTILHAH
                     IHLIPRYMGDVENPRGGIRHTIPGKGYY"
     gene            complement(2920..3993)
                     /locus_tag="PLM68_05475"
     CDS             complement(2920..3993)
                     /locus_tag="PLM68_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOT54849.1"
                     /translation="MYLPYDQNIDAKVLAQIYKNTTATYKFYWFASILDILVKEGKTS
                     MSFWEIIIGMIAEAWYPVHYFRISFGKSDSLSGQIIQLQQALNLPIDSDKELLKRNLA
                     NNLNRPEIKSLLNVFTINVPYRFLSPWINHITNNQVEAQSQTFHNNCMYAIKGEHIHV
                     NPLWLPYLRDNYVILKEFTFWNLTLFLQKRNPNVPDLSSKLVKPIQRDSLLKQRRYWD
                     NFIEVNGPIRCIYTGRPLDKNLYAIDHFIPWSFVSHNLLWNLLPVDPGVNSAKSNDLP
                     RLDIFLKPFAQVHNKVLKTAYYNNPNNKLLEDYLVLYDSVSELVKLSQDDFYRLYKKT
                     FSPLVQIAENMGFKYWKTYNDQH"
     gene            complement(4563..4835)
                     /locus_tag="PLM68_05480"
     CDS             complement(4563..4835)
                     /locus_tag="PLM68_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54850.1"
                     /translation="MGPAIGIGAATTKNRELFWYAEAPGSFAAATVMFCTHLILVGDF
                     NTVACGMLQNTHQGQKHRKSARGGYVFGLTTGKNTAKVPVVGMFLA"
     gene            4886..5719
                     /locus_tag="PLM68_05485"
     CDS             4886..5719
                     /locus_tag="PLM68_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT54851.1"
                     /translation="MTELGKDNKYIDIEKTIRESDSELLTRIPPYAIKWIQSIVKQDE
                     INHILNKYSDYSGKEFLDKIIKEFNVTIEVEGKENLPENDKCFFVANHPYGILDGLIL
                     TYIVSEKYGRLKAIANDAFMLIPQLHPFIAAVNVYGRSSKAYIKALDDTFAMDIPITH
                     FPSGEVSRVYNKKIQDTVWQKSFIKKAISSDRAIVPIYISGRNSALFYTIFLFRRLFH
                     IEVNLELMLLPREIFKKRNKTVKVIIGEQIPHQLFVETKSHQDWAQEVRSRVYNLRNI
                     N"
     gene            5821..6264
                     /locus_tag="PLM68_05490"
     CDS             5821..6264
                     /locus_tag="PLM68_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT54852.1"
                     /translation="MNFGYHDQDGLMTTDEKSEPDKYSLQLYLHLISSVETENKDIVE
                     IGCGRGGGLSHIARNFPIASALGIDLNKRAVRFCNLHYKIDGLTFVRGDAQKLGLENN
                     SCDIVINVESSHCYQNMTLFLKEVFRILRPGGYFRLTDFRYDHEM"
     gene            complement(6491..6572)
                     /locus_tag="PLM68_05495"
     tRNA            complement(6491..6572)
                     /locus_tag="PLM68_05495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6536..6538),aa:Leu,seq:tag)
     gene            6771..8369
                     /locus_tag="PLM68_05500"
     CDS             6771..8369
                     /locus_tag="PLM68_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOT54853.1"
                     /translation="MERSYHNIKTFLSVFVLSFILTECTQEPPAGEEQTLPKLTTGDV
                     TDITTTTASCQCNVTSDGGAAVTARGVCWSTAESPTTAGSKTTDGSGIGSFTGNLTNL
                     TPNTKYYVRAYATNAKGTAYGVQRTFNTLQEQSLATVTTGDISDITTTTAKCAGKVIL
                     DGGAAVTARGICWSTAENPGADGSKTSNGTGTGEFSGSLTELNPNTTYYVRAYAVNSK
                     GTAYGVQKTFTTLQGQTKPAVTTGDITNITATTATCSGNVTSDGGAEVTARGICWSTV
                     ENPGADGSKTSDGTGTGPFSGDLTNLTPNTTYYIRAYATNSEGTAYGEQKSFVTKKDE
                     APDTFKDNRDEKVYKTVTLGKQVWMAENLAYLPSVSPPTEGESDVAFYYVYGYYGTDV
                     AAAKETENYKIYGVLYNWPAAMSACPDGWHLPSNQEWLELINFAGGIQNAGGYLKEAG
                     TEHWREPNEKASNYTGFTGLPGGSRGLDGDFINMFDYGNWWMSTQQGLFDAMFLSLRA
                     HHGDVTRPILPKRNGFSVRCLKDK"
     gene            complement(8469..>8917)
                     /locus_tag="PLM68_05505"
     CDS             complement(8469..>8917)
                     /locus_tag="PLM68_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54854.1"
                     /translation="NTKAWFNKFPGNGGVNFRYNNPKVLRLSEAYLIAAEGALKGSAG
                     ANVASGYLNTIRKRANPDVANVVATDDLIQIERRKELVGEGHRFFDLMRVGKSITRLD
                     SDGHNFAESAGCPGTFDWSYFKIVLPISHTERKFYPELQQNPGYTE"
BASE COUNT         2489 a         1992 c         2137 g         2299 t
ORIGIN      
        1 ggaaacaatc atacaataaa atcagaaaca atcgtacagc aaaaacggaa acaattgtga
       61 cctgcaaaga agattgcatt attgcgccag ttaggcatta ggcctgccca acaactgcca
      121 gtgcttcttt acgaaaaaag cataaagatt aataaaatgc tgacataaat ccttgttctt
      181 ttgtaatatg ttttgtattt tcgtactcaa tgtcaccaaa tactaacaaa aaccattcaa
      241 ctacccaatt ttccgcacaa tcccagcacg agtacatggc tccggacatc atcgtagaag
      301 acctctatgt tgatgtgagc ctccttgatt ccggttccat gaaaaccagt gattgggatg
      361 aaagggattg gtaattgcca ataaaacaaa gatatgaaaa gcttcagcaa gacggccacg
      421 gccgttctga tgttcctttt tatggttgcc tgcaacgtgc aggacaaccc gcaagacagg
      481 caggcatcgc cacaaagtat ccaattcacg ggacggacgg aaaatcctgc caccaggacc
      541 accctggtca atggcattga aacagcctgg gaatccggat ccgacaggat cgggattttt
      601 tcgctccagg cgcgggtgag cgaagagacg gaacctccgg cccacaacgt ggccttctcg
      661 gcccttgaat cggcaaaaac atcggctttc agcggcacca tgctctgggg aacacctgcg
      721 gccgagcacg tctttcacgc ctattacccc taccagtccc tggatgagcc ggccatggat
      781 gcggttcctg tttccctgcc ggccgaacaa atccagccag gtgccaacga cagcggccac
      841 ctctcggccc ttgatttcat ggtatcgacg cccgtggcgg tgtacgcccc ggaatcccct
      901 ggaggagcct ctgtgaacgt ggacctgact ttcaatcacg tgttcaccct catggaatac
      961 cgcatcaccg gcagtggcca ggcattgacg gatatcggcc tggctggggc cggggcgctt
     1021 tctttttcct ccggcaccat ggaccttgac caggaacccg gggcagaccc ttacaccata
     1081 accaaaagcg ggactaacac ctatgtcctg gcacacttgt ctgcatcggc caccctgtca
     1141 cccacaccca ttagtgttta catgatggtg ctgccgggag aacagaccgc agacatgagc
     1201 atcagggtaa aaaccggctg gacctggaaa accctgacca agacgcctcc tgaagggggt
     1261 tttcaaaggg ggagcaagta tgtgacggaa ttggatttgg atgatgccgc tttcaacgac
     1321 gatgagtatg tgtacatacc cgatgataac ttccgggctt attgcctgga caactttgac
     1381 tccgatgacg atggaaaaat aacttttgcc gaggcggagt ccggagcctt gacggtcatg
     1441 aatgtgtcct ccaggagcat catgtctttg gaagggttac agtatttcac ccacgtgaac
     1501 acgttgcaga ctgtggggaa ttcgctgacc tacctggatg tgggcaacaa cctggaattt
     1561 cagacgatca actgtaactc caacaatctg acaactttga aagcaggagg attcaccaac
     1621 ctgaaaatac tgtattgcag ctacaaccaa ttatccaccc tggatttaag cgaaaacccg
     1681 gatctggaaa tattgacctg caaagacaat cttttcactt ctcttgatgt cagcaacaac
     1741 ccggtactgg actaccttga ctgccgcgac aaccccgacc tgcaaacgat ctatgttgcg
     1801 gcagggcaaa ctattagtga cttgtacaag gatgaccata cagtaattgt cacaaggtaa
     1861 acaggagggc tttggggttc cacggcctac aaataagagc cccaccccaa aaacgtatct
     1921 atgcttgcaa aaaagtcaag catgatgaat gatatacgtg cacaaattga cacattggct
     1981 gaaaatacgt ttgacagtta tgtgaatgct tttaaaaagc tatttgttat acaagacata
     2041 gaagcatgga gtcctgctat acgatctgct actgccatat gtagtagatt gaaaagaggg
     2101 ttttctgatc ctccaatagt tattgctgca cttggcctta caccggaaac tctgaaaatg
     2161 gatctcaaga catttggttt tatattcgaa tgcatgtgca tccgcgattt aaaagcctat
     2221 tcgcaggttt ttggcagttc catatcttat tatcatgacc gttataattt agaggctgat
     2281 gcggtgatgc acctgaccga tggaagatat gccctgattg agtttaagtt aggaggcaag
     2341 gaaattgatg agggggctgc tcatttgcta gagatacaaa gtctggtgaa ggaattcaac
     2401 aaggcaaaaa agcagatgaa actgcgtgaa cctgacttgt taatggtaat caccggtggt
     2461 cccattgctt attcacgtcc cgatggcgta aaggtgttgc ctctggcatg tttgagagac
     2521 tgaatttata atgcctcggc ctaataatac cccttaccgg gtatagtatg gcggatgccg
     2581 cctctgggat tttccacatc gcccatatag cgcgggatca ggtgaatgtg cgcgtggaga
     2641 atggtctggc ctgccgccgg acccacgttg atacccacat tgaatccatc gggatggtat
     2701 ctttctgtca ggatggcttt acaacgattc accagctgcc agagttcctg ctgctccgcc
     2761 ccggtaagtt caaaataatc gggaatgtgc tttctgggta tgatcaaggt gtgccccggg
     2821 ctgatggggt agtggtcggg gatggcgagt gctcctgccg attcgcaaat tacttctcta
     2881 tcctctgcaa tggagcagaa gggacattct ttatcagttt taatgctggt cattgtatgt
     2941 tttccaatat ttgaacccca tgttttcagc aatctgcact aatggggaaa atgttttttt
     3001 atataagcgg taaaaatcgt cctgtgaaag tttaaccagc tcggaaacag agtcgtagag
     3061 caccaggtag tcttccagta atttattgtt ggggttgttg taataggcgg ttttgagcac
     3121 cttgttgtgc acttgagcaa agggctttaa aaagatgtcg agccgaggca aatcattgct
     3181 tttcgcggaa ttgacgcccg gatcaaccgg cagcaggttc cacaacaggt tgtgggaaac
     3241 aaaactccag gggatgaagt ggtctatggc atagaggttt ttatctaacg gtctgcctgt
     3301 gtagatacat ctgatggggc cgtttacttc tatgaaattg tcccagtacc tgcgctgctt
     3361 aagcagggaa tcgcgttgaa tgggtttcac cagttttgag gacaggtccg ggacgttagg
     3421 gttgcgcttt tgcaagaaaa gcgtcagatt ccagaaggtg aactctttca ggataacgta
     3481 attgtctctc aggtagggga gccagagggg attgacgtgt atgtgttcgc cttttatagc
     3541 atacatgcag ttgttgtgaa aggtttggga ctgtgcttcc acctggttat tggtaatgtg
     3601 attgatccag ggagaaagga acctgtacgg cacattgatg gtgaaaacgt taagcaaact
     3661 tttaatttcc ggtctgttca agttattggc aagattcctt ttgagcagtt ctttatcgga
     3721 gtcaatagga aggttgagtg cttgttgcag ttgaatgatc tgtccgctga gcgagtcgga
     3781 tttgccaaaa gatatgcgga aataatgaac ggggtaccaa gcctcggcaa tcatgccaat
     3841 gattatctcc caaaaggaca tgctggtttt gccttctttt accagtatgt ccaggatgga
     3901 tgcaaaccaa tagaatttat atgtggccgt agtgttcttg tagatctgag ccagcacttt
     3961 tgcatcaata ttctgatcgt atggcaggta catgcctcta atatacggaa aaacgtggta
     4021 tggcaggtac atgcctctaa tatacggaaa aacgcggtat ggcaggtaca tgcttctaat
     4081 atacggaaaa acgcggtatg gcaggtacat gcttctataa aaacgtgaca ggtggaacct
     4141 cagtgcgcca cttccatttt tcaaaaacgt aaccttcaga attacaagcc attaataaaa
     4201 ttaatcatag ttttggcgta ctgaggttcc acctccgaca cccaccacgg gcacttttgc
     4261 ggtgtttttg cccgtggtgg acttagctaa ccaagggaat gttttgtaaa gtgctttata
     4321 ttagacgttt aacaaaatct acgttctttg gaaattatga ccacgggcac ttttacgggt
     4381 tttttgcccg tggtcaggcc aaaaacatac ccaccacggg cgcttttgcg gtgtttttgc
     4441 ccgtggtgga cttagctaac caagggaatg ttttgtaaag tgctttatat tagacgttta
     4501 acaaaatcta cgttctttgg aaattatgac cacgggcact tttacgggtt ttttgcccgt
     4561 ggtcaggcca aaaacatacc caccacgggc acttttgcgg tgtttttgcc cgtggtcagg
     4621 ccaaaaacat acccaccacg ggcacttttg cggtgttttt gcccctggtg tgtattctgg
     4681 agcataccgc aggccactgt attgaagtca ccaacgagta tcaggtgtgt gcagaacatt
     4741 actgttgctg cagcaaaaga accaggagcc tcggcgtacc aaaaaagctc ccggttcttt
     4801 gtggtggcag caccaattcc aatagcgggg cccacctatt gcctgtctgg aatatataac
     4861 tacatttgaa ggcaatacat tttttatgac agaactgggc aaagacaata aatatattga
     4921 cattgaaaaa accatacgtg aaagtgattc agaattactg acacgtatcc cgccatatgc
     4981 aattaagtgg atccaatcaa ttgtcaagca ggatgaaatc aatcatatcc tcaataaata
     5041 ttcagattat tccgggaaag aattcctgga taaaatcata aaagaattca acgttactat
     5101 tgaggttgaa gggaaagaaa atcttccgga aaacgacaag tgcttttttg tggcgaacca
     5161 cccatatggc atattggacg gattgatact gacttatatc gtgtcagaaa aatacggccg
     5221 tctgaaagcc attgccaatg atgctttcat gttgatccct caattgcatc cattcatagc
     5281 agctgttaat gtatatggca ggtcttcaaa ggcttatatt aaagccttgg atgatacttt
     5341 tgcgatggat attcccataa cgcatttccc gtctggagag gtttcaagag tgtataacaa
     5401 aaagattcag gatacagtct ggcaaaagag ctttatcaaa aaagccattt catctgacag
     5461 ggccattgtc cccatctata tttccggaag aaactcggct ttattctata cgatcttttt
     5521 gtttagaaga ttattccaca tagaggtgaa cctggaactg atgctgttgc ccagggagat
     5581 attcaagaaa aggaataaaa cagtaaaagt tatcatagga gaacaaatcc cacatcaatt
     5641 gtttgtcgaa acaaagtctc accaggactg ggcacaagaa gtgcgttcca gggtgtacaa
     5701 cttaaggaac ataaactaag gcctgctttt ataactttaa aaaacagtct ccgggaaaac
     5761 tgttttcgta tttggtattg gtacgtaaac aaagcagaca agaacgacga gatactttta
     5821 atgaactttg ggtatcatga ccaggatggg ctcatgacaa cagacgaaaa aagtgaacct
     5881 gacaagtatt ccctccagct ttatcttcat ctgataagtt ccgtcgaaac tgagaataag
     5941 gatatcgtgg aaatcggctg tggaaggggt ggcggccttt cccatattgc ccgcaacttc
     6001 cccattgcat ctgccctggg gatagacctg aataaaaggg cagtccggtt ttgcaacctt
     6061 cattataaaa ttgacggttt gacctttgta cggggcgatg cccaaaagtt gggactggag
     6121 aacaattcct gtgacatcgt cattaacgtg gaatcatcac actgttatca aaatatgact
     6181 ctgtttctga aagaagtctt ccggatttta cgtcccgggg gatacttccg gttaacagat
     6241 ttcaggtatg atcacgagat gtgaggacta aaaaaagacc tggaagagag cggattatct
     6301 gtattttttg aaaaaaacat cactcgggaa gttctggctg cccttgcatt ggacgacgga
     6361 cgcaggcgct atcttatcaa gaaactggca cccaaattcc ttcacaatct tgcgctgaat
     6421 tttgccggga ccgtgaggtc gtagacgtaa aaaataaaaa aagagacgaa agattatcct
     6481 tcgtctcttg tgcgggtaaa gggactcgaa cccccacgcc ttgcggcatt agatcctaag
     6541 tctaacgtgt ctaccagttc caccataccc gcaatgtcct gtaaaacaaa acgctggtcg
     6601 ctatttttgc ttctgctggt attttgcagg atggcaaagg taaattattt tttggaatta
     6661 agtcagaagg ttccttctat tgcctgatcc aattataata cttactttta ggggtctaat
     6721 atccacagac ttaagtcccg tagttctttt tcattcgaaa atcaaatcct atggaaaggt
     6781 catatcataa cataaaaacg tttctgagtg tatttgtcct gtctttcata ttgacggaat
     6841 gcacccaaga accgccggcc ggagaggaac agacacttcc caaacttact accggtgatg
     6901 ttaccgatat aactacaaca acggcctcat gtcagtgtaa tgtaacatcg gacgggggtg
     6961 cggcggtaac agcccgggga gtctgttgga gcacggcaga aagtcccacc actgccggat
     7021 caaaaactac agatggctct ggtattggat cctttaccgg gaaccttacg aacctgacac
     7081 caaatacaaa atattatgtg agggcctatg ccacgaatgc aaaaggaaca gcttatggag
     7141 tacaaaggac attcaacacc ttgcaggagc agtctttggc aactgttact accggtgata
     7201 tttcggatat cactactaca acagccaaat gcgccggcaa agttatattg gacggtggag
     7261 cggcggttac agcccgtgga atatgctgga gcacagcaga aaatcctggc gccgatggtt
     7321 caaaaacatc aaatggcact ggtaccggag aattttcagg cagccttaca gagcttaacc
     7381 caaatacgac ttattatgtc agggcttatg cggtcaactc aaaagggaca gcttacggag
     7441 tacaaaaaac atttaccacc ctgcaggggc agactaagcc tgcagttact acgggagata
     7501 ttacaaacat cacggcaaca acagctacct gctcaggtaa cgtgacatcg gacgggggag
     7561 ccgaagttac ggccagggga atctgctgga gcacagtaga aaatcctggc gccgatggtt
     7621 caaaaacatc agatggcacg ggcacaggac ccttttccgg cgatcttaca aatctgacac
     7681 ccaataccac atattacata agggcttatg ccacgaattc ggaagggacg gcttatggag
     7741 agcaaaagtc ttttgtaaca aaaaaggatg aagcacctga cacctttaaa gacaaccgtg
     7801 atgaaaaagt ctacaaaacg gtaaccctgg gcaaacaagt atggatggca gaaaacctgg
     7861 cttatttacc ttcagtcagt cctccaacgg aaggagaatc ggatgtggct ttttactacg
     7921 tttatggata ttatggcaca gatgtggctg ctgccaagga aacagagaat tataaaattt
     7981 acggggtctt gtataactgg cctgcagcca tgtcggcatg ccccgatggc tggcatctgc
     8041 ccagcaatca agaatggtta gaattaataa actttgcagg tggaatacaa aatgccggag
     8101 gctatttgaa agaagcaggg actgaacatt ggagagaacc caatgaaaag gcgagcaact
     8161 acacaggatt tactggcctt ccaggtggga gccgtggcct ggatggagat tttatcaaca
     8221 tgtttgatta tggtaattgg tggatgtcta cccagcaagg attgtttgac gcaatgttcc
     8281 tgagtctccg cgctcatcac ggcgatgtta cgagaccaat tctccctaag aggaatggct
     8341 tttcggtccg gtgcctgaag gataaataaa tagagaccgg ctaaaaattt cttttcagcc
     8401 ggtctccagt cgttccgggc gctccggatc tcaagtgatc taaaacgccc gaagttcaca
     8461 ttgacaattt attccgtata cccgggattc tgctggagtt cgggatagaa cttcctttcc
     8521 gtgtgtgaaa tgggaagcac tatcttgaaa tagctccagt caaacgttcc ggggcatccg
     8581 gcagactcgg caaagttatg cccgtcggag tcgagccggg tgatggattt ccccacgcgc
     8641 ataaggtcaa agaagcggtg tccttccccg acgagctcct tgcggcgttc tatctggatc
     8701 aggtcatcgg tggccactac attggcaaca tcaggattgg ctcttttgcg gatggtgttc
     8761 aggtatccgc tggccacgtt ggcaccggca cttcccttga gggcaccttc ggctgcaata
     8821 aggtaagcct cggacaatct caacactttg ggattgttgt aacggaagtt gactccgccg
     8881 ttgcccggga atttgttgaa ccatgccttt gtgttgt
//
LOCUS       DAOSOF010000077         8895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7830_length_8895_cov_4.806900, whole genome shotgun sequence.
ACCESSION   DAOSOF010000077 DAOSOF010000000
VERSION     DAOSOF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7830_length_8895_cov_4.806900"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLM68_05510"
     CDS             complement(<1..507)
                     /locus_tag="PLM68_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT54855.1"
                     /translation="MGQLNRDLTNGRIGTQLASLTWPTLFGMMGMVIFNLTDTFFLGR
                     LGVKLLAAISFTFPVIMFLNGIGQGIGIGTSSLVSRHVIIAHRDEIRTMASSALLLGL
                     LVVIFFVLFGMLTTRPLFSLLGASGEILEYVHDYMSIWYLGVPFVVLPMVGNNIVRAT
                     GDTFTPGII"
     gene            592..1104
                     /locus_tag="PLM68_05515"
     CDS             592..1104
                     /locus_tag="PLM68_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT54856.1"
                     /translation="MGKEAINVSLVDVQKLISMLNGALAEEWLAYYQYWIGARLMEGP
                     MRLEVEAELLVHANEELGHAVLVVDRIIQLGGTPVINPKDWAKHARCAYEEPSDPYVE
                     RILEQNLAGERCAIQRYQEIADYTNGKDHATYQMAVQILNDELEHENDIESWLADLNR
                     MKEEWKKARM"
     gene            1120..1977
                     /locus_tag="PLM68_05520"
     CDS             1120..1977
                     /locus_tag="PLM68_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOT54857.1"
                     /translation="MCFQTELSLTYTQLENRFNALFREGANYKPGNYSGFSHPRTPVI
                     ANRDAGTIEMMQWGLIPPWATDTEIKEYTLNARLETLRSKPSFRDSVNRRCLVPAGAF
                     YEWQWLDPKGNKKQKYTLTLPGRETFGLAGLWNTWTDRDTGEIMDTYTIITTEANELM
                     SRIHNSKKRMPVLIDREHEPDWLENGEMVMQNDRLQASRDPLLTIPGIGTSMAEDLRV
                     LGIETVADLTGKDPQQLYDRLCAIRKTRLDPCVLYVFRCAVYFAGTNNHDPELLKWWN
                     WKDKMNDDV"
     gene            1967..2911
                     /locus_tag="PLM68_05525"
     CDS             1967..2911
                     /locus_tag="PLM68_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT54858.1"
                     /translation="MMYKHLFGPVPSRRLGMSLGVDMVPRKVCSLDCVYCEVGRTTLL
                     TTERKEYVAEREIKEELNDFFSSHPDPEIITFSGYGEPTLNSAIAGVIDFLKEKKSQL
                     PIAILSNGTLMGDPAVRKAILRADIVLPSLDAATEEVFRKINRPAGALDLQGYIQGLA
                     DFRKEFSGKIWLEVFILPGFNDHKAELDALKKAILKIKPDSVQLNTLDRPGTEAGIRS
                     ASRKELEDIMAYWDMPRVEIIASSVANKEPAAYREDVQNAILETINRRPCTLDDIALI
                     TGLHVNEINKHLRTLEATGSIRTERQERGLFYMPSQRN"
     gene            2943..4253
                     /locus_tag="PLM68_05530"
     CDS             2943..4253
                     /locus_tag="PLM68_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT54859.1"
                     /translation="MERLDNWVEKIIMNLGVSESAAPILRLLLLLLVLGLLSWAFFRV
                     TRLIIKGPLFRYVKRTQSKWDDLLMDNKVFSNLAHIVPVIMVRVIAPILFRDFEKVLP
                     VVIKLTDIYLIIVILLAINALLRILELVLKKSKAFADKPVSSYFQLIKIILYIAAGIL
                     IISILINKSPVYLLSALGAMTALGMLVFKDTILGFVASVQISANDMVRIGDWVEMPKF
                     NADGDVIAINLNTVKVQNWDKTITTIPTYYFITDSFKNWRGMKETGGRRIKRSIYINA
                     ASVKYINEEDRLRYARLYLLKDYLVTRQKEIDEYNKKMKVDTSVFVNGRRMTNIGVFR
                     HYIENYLKDNKAIRKDMTLMVRQMAMEDRGIPIEIYCFTTTTVWTEYEEIQSDIFDHL
                     MAAVSFFDLEVFQQPSGSDLKRAFLPAGKIITSGFQEKESNPSA"
     gene            complement(4210..4899)
                     /gene="mtgA"
                     /locus_tag="PLM68_05535"
     CDS             complement(4210..4899)
                     /gene="mtgA"
                     /locus_tag="PLM68_05535"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOT54860.1"
                     /translation="MKIRKIYRWLLWLVAGLFALSLLLVILYRFVPVPYTPLMAIRAV
                     QQKKSEGKAVLHHQWVPLEDISANMMMAVVASEDQRFFEHIGLDMEQIKKARKENLTR
                     RRPRGASTISQQTAKNVFLWPRSSWFRKGLEVYFTFLIELFWSKERILEVYLNSIEMG
                     KGIYGAEAVARQHFNTSASQLTRYQAALIAATLPNPLRFSSRNPSAYIQRRTQEILRQ
                     MDLIPFPENLR"
     gene            complement(4889..5335)
                     /locus_tag="PLM68_05540"
     CDS             complement(4889..5335)
                     /locus_tag="PLM68_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54861.1"
                     /translation="MTQTLFNEYNKPSAGERKAMTDFLHAHLEEFRDSKEDIDCAMAY
                     ILREIPSPGGFILRLTEDQAIVCVVMIAKTGMKGFVPENLVVYLATHREYRGRGIGRK
                     MMQLAMDAAEGNLALHVEPHNPARKLYEDLGFTNKYLEMRCIRREN"
     gene            complement(5332..6201)
                     /gene="prmA"
                     /locus_tag="PLM68_05545"
     CDS             complement(5332..6201)
                     /gene="prmA"
                     /locus_tag="PLM68_05545"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT54862.1"
                     /translation="MEQVAVTLVIEPYCQETEEILIAELSEIGYEGFVSADNGLTAYI
                     SSAEFRESHLRLILDTYGITGFSKEYIPEKNWNADWDIRFNPVILKTGKGCSIRSAGN
                     GAMIPVWPPVAYKLVIMPELAFGTGHHPTTRMMLESLLEMEQQGFIKNRRVLDMGTGT
                     GVLAILAAKMGAAVPVHALDNDRRSVHSCNENARRNRIAHKVHVLHGDASFIQSGRYG
                     LILANIHRNILISEMDTLARGLEPGRGDLLLSGFFTTDVPAMMEAAQQHGLVLQKQKE
                     KEGWSLLHLSKTS"
     gene            complement(6207..6965)
                     /gene="tpiA"
                     /locus_tag="PLM68_05550"
     CDS             complement(6207..6965)
                     /gene="tpiA"
                     /locus_tag="PLM68_05550"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT54863.1"
                     /translation="MREKIVAGNWKMNTTLKEGKKLIEEILSGTDRIPQDVTLIIAPP
                     FTHLCPAARLIGNRRNPALAAQNSANRESGAYTGEVSASMLASIPCSHVILGHSERRE
                     YYGETNEILLDKIRLALASRLRPIFCVGEVLAEREGSMHFQVVASQLREVLCQLGPED
                     FKKVIVAYEPVWAIGTGKTATAEQAQEMHAFIRAFLAGTFGDLAASTPILYGGSCKPS
                     NAREIFSQPDVDGGLIGGASLKAEDFLAIAGSFA"
     gene            7074..8369
                     /gene="tig"
                     /locus_tag="PLM68_05555"
     CDS             7074..8369
                     /gene="tig"
                     /locus_tag="PLM68_05555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT54864.1"
                     /translation="MNITKKEIDDLSLQVTVSVEENDYSEKVKKALNDTRKKIEIKGF
                     RKGMVPLGIVQKMYGRSILLEQVNTYISEALNAYMEKEAIRIIGEPLVSEEQAKTDWD
                     KDVDFSFSFDLMLAPKVDVPINKDLHIPLIKKNVSEKDVERQLETVLEQQGTLQVAEK
                     VEKEDLLKVDLRQGELLISDSYLNLKNVAKQKDKKPFIGKAVGEEAETDISVLFPKEA
                     DRAILLQIKQEELERFTNPVFTFVIKEIKRFEPARENQELYDKLFGPGNVTTHESFLE
                     KIREQIDLNNQSESNYRFGQDVKNILVEKAGLQLPEKLLKRWLFEGNDGKFTMEQIEK
                     DFPAFLEDFRWQLVRETIMKEYGLKVEKDDMAQSAVAMARYQFAMYGMNNVPDEHLIK
                     YAERMLANEKEARNLYERAEENKVISYIRENVTVDIKEE"
     gene            8373..>8895
                     /locus_tag="PLM68_05560"
     CDS             8373..>8895
                     /locus_tag="PLM68_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT54865.1"
                     /translation="MTEFEKYAIRHKGISSMSLHRYQSLYSTPQGSITPYIIEERQLN
                     VAQMDVFSRLMMDRILFLGVPILDEVANIIQAQLLFLDSTDPGKDVQIYINSPGGSVT
                     AGLGIYDTMQLVHSEVATICTGQAASMAAVLLAAGKKGKRSALPHARVMIHQPVGGVE
                     GQAADMAIAVKEIL"
BASE COUNT         2340 a         2144 c         2338 g         2073 t
ORIGIN      
        1 gataatgcca ggggtgaagg tatctccggt tgcccggact atgttgttcc ccaccatggg
       61 caggaccacg aacgggaccc ccaggtacca gatggacatg taatcgtgca cgtactccag
      121 gatttcaccc gatgccccca gaagggagaa caagggtctg gtggtcagca tgccaaacaa
      181 gacaaaaaaa atgaccacca gcaatcccag caagagcgcg ctactggcca tggtcctgat
      241 ctcatcccgg tgagcgatga tcacatggcg cgacaccagg gaacttgtcc ctatcccgat
      301 tccttgtcct attccattca ggaacatgat gaccggaaaa gtgaaactta tggcagccag
      361 cagcttcaca cccagccggc ccaaaaagaa tgtatccgtc aggttgaaaa tcaccatacc
      421 catcatgcca aacaacgtgg gccatgtaag cgatgcgagt tgtgtgccta tacggccgtt
      481 tgtaaggtcc ctgttcaatt gccccataag atgaaaatcc ttgaatagcc caaaaaacaa
      541 atattttttg taagtttgaa acttattaat aataactaaa attacaattt tatgggaaaa
      601 gaagcaataa atgtatcgct tgttgatgtt caaaaattaa taagtatgct gaatggtgca
      661 ctggcagaag aatggctggc ttactaccag tactggatcg gcgcaaggct tatggaaggc
      721 cccatgcgcc tggaggtgga agccgagttg ctggttcatg ccaatgaaga actcgggcac
      781 gctgtcctgg tggtggaccg catcattcaa ctggggggga ctcccgtcat caatcccaaa
      841 gactgggcaa aacacgcccg ttgcgcttac gaggaacctt cggatcctta cgttgaaaga
      901 atcctggaac aaaacctggc cggtgaaaga tgtgctattc aaaggtacca ggaaattgcc
      961 gactacacca atgggaaaga ccacgccacc taccagatgg cagtacagat cctgaacgac
     1021 gaactggaac acgaaaacga catagaatcc tggctcgccg atctgaaccg gatgaaggaa
     1081 gagtggaaaa aggccaggat gtagtcctga catgaactca tgtgctttca aacggaacta
     1141 tctctgactt atacacagct ggagaacagg ttcaatgcct tgttccggga aggagcaaac
     1201 tataaacccg ggaactattc cgggttttcc catcccagaa ccccggtcat agccaaccgg
     1261 gatgcgggaa ccatagaaat gatgcaatgg ggtctgatcc cgccctgggc cacggatacg
     1321 gaaattaaag aatatacgtt aaacgcccgc ctggaaacgt tgcgttccaa accttcgttc
     1381 agggattcgg tcaacaggcg gtgtctggtg ccggccggcg ctttttacga gtggcagtgg
     1441 cttgatccca aaggaaacaa gaaacagaaa tacacgctga cacttcccgg gagagagacc
     1501 ttcggcctgg cgggattgtg gaacacttgg acggacaggg acaccgggga gataatggat
     1561 acttacacca tcattaccac agaagccaac gaactgatgt cacgcattca caattcaaag
     1621 aaaaggatgc cggtgctgat tgacagggag catgagccgg attggctgga aaacggggaa
     1681 atggtcatgc agaacgaccg cttgcaggca tcgcgggatc cgctgcttac aattccgggc
     1741 ataggaacaa gtatggcaga ggatttgagg gtactgggca ttgaaactgt ggcggacctg
     1801 acgggtaagg atccgcagca gttgtacgac cggttgtgtg cgatcaggaa aaccaggctg
     1861 gatccctgcg tgctttatgt tttccggtgt gccgtatact ttgcaggcac caacaaccac
     1921 gacccggagt tattgaaatg gtggaattgg aaagataaaa tgaacgatga tgtataagca
     1981 cctttttgga ccggtacctt cgcgacggct gggcatgtcg ctgggtgtgg acatggtgcc
     2041 caggaaagtc tgctccctgg attgtgtata ctgtgaggtg ggccgcacca cattgcttac
     2101 cacggaaagg aaagaatacg ttgccgaacg ggagatcaaa gaggaattga atgatttctt
     2161 ttccagccat cccgatcccg aaatcattac cttttcgggg tacggcgaac ctaccttgaa
     2221 cagtgccatt gccggtgtca tagattttct gaaggaaaag aaatcgcaac tgcccattgc
     2281 catattgagc aatggcacat tgatgggcga ccctgcagtt cgcaaggcca tactgagggc
     2341 cgacatagtg cttccgtcac tggacgcagc cacagaagag gtgttcagga agatcaaccg
     2401 gcctgcagga gcactggacc tgcaaggcta tattcaaggt ctggcggatt tcaggaaaga
     2461 gttttccggg aaaatatggc tggaagtatt cattcttccc ggcttcaatg accataaagc
     2521 cgaactggat gcactgaaaa aagccatcct gaagattaaa ccggattccg tgcagcttaa
     2581 taccctggac aggccgggga ccgaagccgg catcagaagc gccagccgca aggaactgga
     2641 ggacataatg gcgtactggg atatgccccg tgtcgagatc atcgcctcgt cagtggccaa
     2701 caaggaacct gcagcataca gggaagatgt gcaaaatgcc atcctggaga ccataaaccg
     2761 caggccctgc accctggatg acattgcact cataacaggc ctgcatgtga acgagatcaa
     2821 caaacacctg cgtaccctgg aagcaacagg ctctatccgc acagaaagac aggaacgggg
     2881 tttgttctac atgccttcac aaagaaatta aataaattca acgatcataa aacgggagaa
     2941 ttatggaaag acttgacaat tgggtggaga aaataatcat gaatctgggg gtatcggaaa
     3001 gtgcggcacc tatcctgaga ctgcttcttc tgctgttggt gctgggactc ctttcgtggg
     3061 cctttttccg cgtcacccgc ctgatcatca aaggaccgtt gttccggtat gttaagcgta
     3121 ctcagtccaa gtgggatgac ctgctgatgg acaataaagt tttttccaac ctggcacaca
     3181 ttgtccccgt tatcatggta cgcgtgattg cacccatcct gttccgggat tttgagaagg
     3241 tcctccccgt tgtgatcaaa ctcacagaca tctacctgat cattgtcata ctattggcaa
     3301 tcaacgcctt gttgcgtatt ttggagctag tgctgaagaa atccaaagct tttgcggaca
     3361 aaccggtgtc cagttacttt cagctgatca agatcatttt atacatagct gccgggatcc
     3421 tgataatatc aatactcatc aacaaatctc ccgtatacct gctcagtgcc ctgggtgcca
     3481 tgaccgccct gggcatgctg gtcttcaagg ataccatcct gggatttgta gccagtgtgc
     3541 agatttcggc aaatgatatg gtacgcatag gggattgggt ggaaatgccc aagttcaacg
     3601 cggacggcga tgtcattgcc atcaacctga acacggtcaa ggtacagaat tgggacaaga
     3661 ccatcacaac catccccacg tattatttca ttacggacag cttcaagaac tggcgcggca
     3721 tgaaagagac agggggcagg aggataaaaa gatccatata catcaatgct gcctcggtca
     3781 agtatatcaa tgaggaagac aggctcaggt atgcaaggct ctatctgctt aaggattacc
     3841 tggtcacacg gcagaaggaa attgatgaat acaacaagaa aatgaaggtt gacacgtctg
     3901 tgttcgttaa cggacgccgt atgaccaata tcggggtatt ccgtcattac atagagaatt
     3961 acctgaaaga caacaaagcc atcagaaaag acatgacttt gatggtaagg cagatggcaa
     4021 tggaagacag gggaatcccc attgaaatat actgtttcac cacaaccacc gtctggacgg
     4081 aatatgaaga gatccagtct gacatctttg atcatttgat ggcagcagtg tctttctttg
     4141 acctggaagt attccagcaa cccagtggtt ctgacctgaa acgggccttt ttgccggcag
     4201 ggaaaattat tacctcaggt tttcaggaaa aggaatcaaa tccatctgcc tgaggatctc
     4261 ctgcgtgcgc ctctggatgt aagccgaggg attgcgtgag ctgaaccgca ggggattggg
     4321 aagggtggcg gcaatcaggg ccgcctggta tcgcgtcagc tgcgatgccg atgtgttgaa
     4381 atgctgccgc gccacggcct cggccccgta aattccctta cccatctcaa tggaattcag
     4441 gtagacttcc aggatccttt ccttgctcca gaacagctca atgaggaagg tgaaatacac
     4501 ttccagtcct ttccggaacc aggagctccg tggccagagg aagacatttt ttgccgtttg
     4561 ttgcgagatg gtgctggctc ccctggggcg gcggcgggta aggttttcct ttctggcctt
     4621 tttgatctgt tccatgtcaa gaccgatgtg ctcaaagaaa cgctggtctt ccgaggcaac
     4681 caccgccatc atcatgtttg ccgagatgtc ctccaggggg acccattggt ggtgcaggac
     4741 ggctttccct tcgcttttct tttgctgcac ggcacggatg gccatgagag gggtatacgg
     4801 gacgggaacg aaccggtaca ggatcaccaa cagcaggctc agggcaaaca agccggccac
     4861 cagccacaac agccaacgat agattttcct aattttcacg ccgtatacac ctcatttcaa
     4921 gatacttgtt ggtaaagcca aggtcctcgt acaatttcct ggccgggttg tgcggttcca
     4981 cgtgaagtgc caggtttcct tctgcggcat ccatggccag ttgcatcatt ttcctgccta
     5041 tgccccgtcc tctgtattcc ctgtgtgtgg ccaggtatac gaccaggttc tccggcacaa
     5101 aacccttcat gcctgtcttg gctatcatga ccacgcatac aattgcctgg tcttcggtca
     5161 ggcggaggat gaagcctccg ggtgaaggga tttcccttag tatgtatgcc atggcgcagt
     5221 cgatatcttc tttgctgtcc ctgaactctt ccaggtgtgc atgcaggaaa tccgtcatgg
     5281 ctttcctttc cccggcggat ggtttgttat actcgttgaa tagggtctgt gtcatgatgt
     5341 tttgctcagg tggagcaggg accacccttc cttttctttt tgtttctgca ggaccagccc
     5401 gtgttgttgg gcggcttcca tcattgccgg gacatctgtc gtaaagaaac cgctcaaaag
     5461 caaatcacct cttccgggtt ccagtccacg tgccaatgtg tccatttcac tgataaggat
     5521 gttgcggtgg atgttggcca gtatgagtcc gtacctgcct gattggatga aagaggcatc
     5581 accgtgaagg acgtgcactt tgtgtgcgat gcggttcctg cgtgcatttt cattgcatga
     5641 atgtacgctg cgacggtcat tgtccagggc gtggacagga acggccgctc ccattttcgc
     5701 tgccaggatg gccaggacgc ctgtcccggt tcccatatcc agcaccctcc tgtttttgat
     5761 gaaaccctgc tgttccatct ccagcaggct ttccagcatc attcgtgtag tggggtggtg
     5821 accggtgccg aaggccagtt ccggcatgat gaccagtttg tatgccacag gcggccatac
     5881 gggtatcatg gccccgttgc cggcggagcg tatgctgcat ccttttccgg tcttcaggat
     5941 aacgggattg aaacgtatgt cccagtctgc attccagttt ttttcaggga tgtattcctt
     6001 tgaaaaacct gttattccat aggtgtccaa gattagcctc aggtgggatt ccctgaattc
     6061 agctgaagaa atgtaggcag tcagaccgtt gtctgcagac acgaatcctt catagccaat
     6121 ttcggatagt tcggctatca ggatttcttc tgtttcctgg caataaggtt caatgactaa
     6181 ggtgacggct acctgttcca tacttttcag gcaaacgatc cggcaatagc cagaaagtcc
     6241 tcggctttca gcgaagcgcc tcctatcagg ccgccgtcca catcgggctg ggaaaagatt
     6301 tcgcgggcat ttgacggttt gcagctgcct ccgtacagta tgggagtgga ggctgcaaga
     6361 tctccgaatg tgccggccag gaaagcgcgg atgaacgcat gcatttcctg tgcctgttct
     6421 gcagttgccg tttttccggt gccgatggcc cagacgggtt cgtaggccac gatgaccttt
     6481 ttgaaatctt ccggtcccag ttggcacagg acttccctca attgggaggc aactacctgg
     6541 aaatgcatgc tgccttcacg ctctgccagc acttctccca cacagaatat gggcctgagc
     6601 cggctggcaa gtgccagtct gattttatcc aacaggatct cattggtctc accataatac
     6661 tccctgcgtt cagaatgccc caggatcaca tgactgcagg ggatggaggc aagcatggaa
     6721 gccgagactt caccggtata ggctccgctt tccctgttgg cagaattctg cgcggccagt
     6781 gcgggattcc tgcggtttcc tatcagacgg gctgccgggc acaaatgcgt gaagggcggg
     6841 gctattatca gggttacatc ttgcggtatg cggtcggttc ccgaaaggat ttcttctatc
     6901 agctttttcc cttctttcag ggtggtgttc attttccagt ttccggcaac gattttttct
     6961 ctcatagatt ccaatgataa ttagtggtgt atgcaatggc gacaaaggta agaaaatgat
     7021 tggttttttg acgattaata cctacttttg cagcccctta taagatcaaa catatgaata
     7081 tcactaagaa agaaattgac gacctttccc tgcaggtgac cgtatcggtt gaagaaaacg
     7141 actacagcga gaaagtgaaa aaagccctga atgatacccg gaaaaaaata gagataaaag
     7201 gtttcagaaa agggatggtg cctttgggaa ttgttcagaa aatgtatgga cgttccatat
     7261 tgctggaaca ggttaacacc tacatatcgg aagcgctgaa cgcatacatg gaaaaagaag
     7321 ccatccgcat tatcggtgaa ccgctcgttt ctgaagagca ggccaagaca gactgggata
     7381 aagacgtgga tttttcgttt tcatttgacc tgatgctggc cccaaaggta gatgtcccca
     7441 tcaacaagga tcttcatatt ccgctgataa agaaaaacgt aagcgaaaag gacgtggaac
     7501 ggcagctgga aaccgtactg gaacagcagg gaaccctgca ggtggcagag aaggtggaaa
     7561 aagaagacct tcttaaggtt gacctccgtc agggagaact gctcataagc gattcgtatc
     7621 tgaacctgaa aaacgttgca aagcagaaag acaagaagcc tttcattgga aaagccgttg
     7681 gtgaagaagc ggaaacagac atcagcgtct tgtttcccaa agaagcggac agggccatat
     7741 tattgcagat caagcaggag gagctggagc gttttactaa cccggtgttt acttttgtga
     7801 taaaggagat caagcgtttt gaaccggcac gggaaaacca ggagttgtac gacaagctct
     7861 tcggaccggg aaatgtcaca acacatgaaa gcttccttga aaagatacgt gagcagatag
     7921 atttgaacaa ccagagcgag agcaactacc gtttcggcca ggatgtgaag aacatactgg
     7981 tggaaaaagc cggtctgcag cttcctgaaa aactccttaa acgctggttg ttcgaaggca
     8041 acgatggtaa gttcaccatg gaacagatag aaaaggattt tcccgcattt ctggaggatt
     8101 tccgctggca gctggtcagg gaaaccatca tgaaagagta tggcctcaag gttgagaaag
     8161 atgatatggc ccagagtgcc gtggccatgg cacgttacca gtttgccatg tacgggatga
     8221 acaatgttcc cgacgaacac ctgataaaat atgcggaaag gatgctggcc aacgagaaag
     8281 aagccaggaa tctgtacgag agggcagagg agaacaaagt tatttcgtat attcgcgaaa
     8341 acgtaacagt cgatatcaaa gaggaataac ttatgaccga gtttgaaaaa tacgccatca
     8401 gacataaagg tatcagcagc atgagcctgc acaggtacca gtctttgtac agcactccgc
     8461 agggatccat aacgccctac atcattgagg aacgtcagtt gaacgtagcc cagatggatg
     8521 tgttcagccg tctgatgatg gaccggatcc tttttctggg ggttcccatc ctggacgagg
     8581 tggccaatat tatccaggcc cagctgctgt tcctggattc gacagacccc gggaaagacg
     8641 tccagatata tatcaattca ccgggaggat cggttaccgc aggattggga atctacgaca
     8701 ccatgcagct ggtgcattca gaagttgcca ccatatgtac gggtcaggca gcgtctatgg
     8761 ccgcggtttt gctcgcagcc ggaaagaaag gcaaacgctc ggcattgcct cacgcccggg
     8821 tgatgatcca ccagccggtg ggcggtgtgg aaggacaggc agccgacatg gccattgccg
     8881 tgaaagagat cctgg
//
LOCUS       DAOSOF010000078         8889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7833_length_8889_cov_5.461399, whole genome shotgun sequence.
ACCESSION   DAOSOF010000078 DAOSOF010000000
VERSION     DAOSOF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7833_length_8889_cov_5.461399"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLM68_05565"
     CDS             <1..223
                     /locus_tag="PLM68_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54866.1"
                     /translation="TEVYFLEDPSLVSDGLDTPAPECTRITAYLAIQAIPDMASILEQ
                     AAETAARLLQPLPGENSVYTGNHLVAMRL"
     gene            279..2717
                     /locus_tag="PLM68_05570"
     CDS             279..2717
                     /locus_tag="PLM68_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54867.1"
                     /translation="MKKFLISFFVLLPYFVNAQTFHGRVLLSGENGDIQPAAAAVVYW
                     VPERKGGPVEHVVYTGDDGSFTIPAGRTGMLTASYIGYIEDTIQISPAQEDIEFILWE
                     NAGTLDMLHITGRRQSNYISRLTPVKTEVITAAGLCKMACCNLAESFENSASVSVGFT
                     DAVTGARQIRLLGLSGNYTQMLEENRPAMRGIQGPFGLSFIPGQWLESIQIAKGNGSV
                     VNGCEAITGQINMEFRKPTAELPLFVNLFLNDALRTEANMASSLQLNDRWSTVVLAHG
                     SADLMRHDSNGDGFLDEPLTNRYSLANRWLYADPSGLQLRFGFRGLYEDRKSGEMDND
                     WVLPGQARDNAIWGSRIINEGVNAYVKLGIPLVKHDHAHDHAQEQLPMHGQEQEVAHG
                     QEAEYVHGEDYDHEYETAETTGQEEVTEAVENDNHIDPNIAMVLDYNLHKQDAYFGLK
                     DFNALQSDFFANAIFMSSLGKHHKVTAGVSALVKNLDQVLTDRWPAGGEESFDLGRKE
                     GVYGAYGEYTLEWGDKLVFIAGARADYNTLYGWLFTPGVNLRYNFTENLVFRANAGRG
                     YRTPYSITDNIGVLSTGRRIVFGETPEMEEAWTYGLNLTLVLPFGHGGSSTLSLEYFR
                     TDFINQLIADQEYLWNAQVPSILFYNLEGRSYTNTWQADFTTEPFDGFSVLATFRYND
                     SKVQLKGQGLVDRPLTSKFKGVLNLQYTTPRDTWVFDVTGQINGPARIPSFAADTEEK
                     TSPVFPMFYAQVTRKLKNMDIYIGGENLMNYRQPDPIISADDPFSPQFNASIIWGPLM
                     GIRVYAGMRLSLFR"
     gene            2768..5314
                     /locus_tag="PLM68_05575"
     CDS             2768..5314
                     /locus_tag="PLM68_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOT54868.1"
                     /translation="MTRSMTCWLICLLFFQIMTAQETGAWIRINQAGYLPGDIKVAVL
                     ISKEEASPVAFRVLDMRTDACVFSGSVEEGTIKEVPAVKWGMASAFRLDFSELKEEGG
                     YRVVTDIPGKGTVESPAFRIGAEVYEGTADFLLTYMRQQRCGDNPFLDTLCHQNDGYI
                     VLHPERTGEKIDVRGGWHDATDYLQYLTTSANATFQMMFAYTQAEDKSVFKDRFDATG
                     RPGVNGIPDILDEVRWGLEWLLKMNPEDKMMFNQIADDRDHVGFRLPVGDKADYGWGP
                     GGGRPVYFITGERQGLREHINRTTGVASSAGKFASAFALGAELFRELDPGFAEKMKAK
                     ALPAYHFAEEKPGNTQTCCVVSPYFYEEDNYVDDVELAAAVFHHLGAGEEWLQKADYW
                     GQLEEVTPWMELGRARHYQFYPFINLGHYYLASSDTPMAEKYTEYMRRGLEHIRQRSS
                     DCAFMNGIPLMWCSSNMVAAAITQADLYYRLTGDSTYREMEASLRDWLFGCNPWGTSM
                     IVDFPRGGDYPKRPHTSYLPTLGKSTPGGLIDGPQLRERLKEHSQYISLADGAASYAP
                     FNQGVALYHDETWDYATNEPTMDGTASLTYYLAGREAEGRKEAAGLAADKSRPATERD
                     QRGGIIRMNPGTPEIYLTFTADERFEGGRTVLETLKRNGIKASFFLTGKCLRDPQWKE
                     LVQDIISQGHYVGPHGDMHLQYAPWDGDIEQSLVSGDSLRNDIACNMDELSRVGLDVS
                     KIRWFMPSYEHFNTETVRVTASMGMEVIHLTPGIMTLADYTTPDMPSYRSTKELLKQL
                     YDLEREKGLHGAIMLIHPGTEPSRTEKLYDRLDQIIKTLKRKGYVFRRLP"
     gene            5371..6231
                     /locus_tag="PLM68_05580"
     CDS             5371..6231
                     /locus_tag="PLM68_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT54869.1"
                     /translation="MYKILKKRILAPDICLMEVSAPRVASSAQPGQFLIVRVGEKGER
                     IPLTICDFDPVAGSVTIVTQRVGASSRKIFALEEGESFNDFAGPLGVPSEFIHEREEI
                     LKEKKFLFIAGGLGTAPVYPQVKYLHGKGAHVEVIIGAKTRDLVIMEEEMKQVAARVH
                     ICTDDGSYGFKGLVTARLKELLDGGARFDQAVAIGPMIMMKFVTLTARKYNLPLIVSL
                     NTLMVDGTGMCGACRVTVGGKIRFACVDGPEFDAYQVDFDEAMRRQSMYKLQEKDADH
                     KCKIGLGNGQ"
     gene            6221..7600
                     /gene="gltA"
                     /locus_tag="PLM68_05585"
     CDS             6221..7600
                     /gene="gltA"
                     /locus_tag="PLM68_05585"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT54870.1"
                     /translation="MVNKIRVPVREQDPKARTGNFQEVSYGYNAQEAVLEASRCIQCK
                     NPKCVTGCPVTIQIPQFIKAILEGDMPGAASVIARDSSLPAVCGRVCPQESQCEGVCV
                     MGIKNDPIAIGKLERYVADWARENNYTTAIPAVPNGRKVAIVGSGPAGLACATDLAKA
                     GCDVTIFEALHKPGGVLVYGIPEFRLPKAKVVDYEIGQVRKLGVKIECNVVVGRTVTI
                     DQLFDQEGFDAVFVGSGAGLPKFLGIPGENLNGVVSANEFLTRNNLMEAYVPESDTPI
                     YVGKRVAVVGGGNVAMDAARTALRLGAEVEVVYRRTRKEMPARVEEVHHAEEEGVTFQ
                     MLTNPVAVLGNEKGWVTGLRCIRMELGEPDESGRRRPVEVPGSEFDIACDVTVIALGT
                     SSNPLIARTTPGLEMNRHNGIVADERGITSRPGVFAGGDVVTGSATVILAMGAGRQAA
                     KSILEYLEA"
     gene            complement(7545..>8889)
                     /locus_tag="PLM68_05590"
     CDS             complement(7545..>8889)
                     /locus_tag="PLM68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT54871.1"
                     /translation="YRKQAQKDSPSGTGIQDSACQAGYRAIARDAAAEGMVLLENRNA
                     LPFNDSIRRIALFGVSSYRLVAGGFGSGEVYADHTVSPAEGLQEAGYLPDPQLRERYQ
                     SYTDSVNRAYKRKWKTITQRITGQGLPTETDPGAEAIARSAVENQVAIITIGRRTGEF
                     TDRTIEGDYLLSEEEKALIGNVCKAFQAVGKPVVVVLNVGGIVETASWSGLPDALLLA
                     WMPGQEGGRALADIVSGKKTPCGRLPVTIPAYLEDLASHKNFPLEGVPVKWLSMMGKK
                     EKKTAAERLNIDYTRYEEGIFVGYRDVDSHRKEVAYHFGYGLSYTSFLYAKPDVRVEP
                     ERIVVQCVITNTGEKNGREVVQAYVSAPEGMGDKPYQELKGFAKTPLLGPGESCMAEI
                     IIPMERLASYVPGTGWVVDAGDYTLRIGSSSRNMQQEIRLPGIPGLILPPGGRLP"
BASE COUNT         2146 a         2285 c         2472 g         1986 t
ORIGIN      
        1 tacagaggtg tatttcctgg aagatccctc actggtttct gacggattgg acacacctgc
       61 ccccgaatgc accaggatta ccgcctacct ggcaattcag gcaatcccgg acatggcttc
      121 aatcctggaa caggcagcgg aaacggcggc cagactgctc cagcctttgc caggggaaaa
      181 ttctgtttat actggtaatc atttggtagc tatgcgttta taatgaattg cactgcatcg
      241 gtcaagtcaa ttcattattt aatcaagcgc aagtatccat gaaaaaattc cttataagtt
      301 tctttgtttt attaccatat tttgtaaacg cccagacatt ccatggcagg gtattgcttt
      361 ccggtgaaaa cggggacatt cagccggctg ctgcggccgt tgtatattgg gttcctgaac
      421 gcaagggagg acctgtggaa catgtggtat ataccggtga cgacggctct ttcacaattc
      481 ctgccggtag aaccggaatg ctgacagcat cctacattgg ctatattgaa gacaccatcc
      541 agatcagtcc cgcacaggaa gacatagaat tcatcttgtg ggaaaatgcg ggaaccctgg
      601 atatgctgca tatcacagga cgaaggcagt caaattacat ttcccgcctg actcccgtga
      661 agaccgaggt gatcaccgca gccggcctgt gcaagatggc ctgttgcaac cttgcagaga
      721 gttttgaaaa ctcggccagt gtatctgtcg gctttaccga tgccgttacc ggagcccgtc
      781 agatccgcct cctggggctg agcgggaatt atacacagat gctggaagag aaccggcctg
      841 caatgagggg catccagggt cctttcggcc tttccttcat tcccggtcaa tggctggaaa
      901 gcatacagat agccaaggga aacggatctg ttgtgaacgg ttgcgaagcc atcaccggcc
      961 agatcaatat ggaattcaga aaacccaccg cggaattacc gctttttgtg aaccttttcc
     1021 tgaacgatgc attgcgcact gaggctaata tggcttcttc ccttcaatta aatgaccggt
     1081 ggagcaccgt tgtcctggcc cacggttctg cagacctgat gaggcatgat tccaacggtg
     1141 acggcttttt ggatgaaccg ttgacaaacc gttattcctt ggcaaacagg tggctctatg
     1201 ccgatccctc gggtcttcag ctgcgttttg gattcagggg cctgtatgaa gacaggaaaa
     1261 gcggggaaat ggacaacgac tgggtattgc ccggtcaggc cagggacaat gccatatggg
     1321 gttcccggat tatcaatgaa ggcgtgaatg cctatgtgaa actaggcatt ccccttgtaa
     1381 aacacgacca cgcacatgac cacgcccagg aacagttacc catgcatgga caggagcagg
     1441 aagttgctca cgggcaggaa gcagaatatg tgcacggcga ggattacgac catgaatatg
     1501 aaactgcgga aacaacaggc caagaagaag ttacagaagc tgtagaaaat gacaaccaca
     1561 ttgacccgaa tattgccatg gtgctggatt acaacctgca caaacaggac gcctactttg
     1621 gcctgaaaga tttcaatgcg ctgcaaagtg atttttttgc caacgcaatc ttcatgtcga
     1681 gcctcgggaa gcatcataag gtcacggccg gtgtaagcgc cctggtgaag aatcttgacc
     1741 aggtgctgac agaccggtgg cctgccggcg gggaggaatc ctttgacctg ggccgcaagg
     1801 aaggtgtcta tggagcttac ggggaataca ccctggaatg gggcgacaag cttgttttca
     1861 tagccggggc aagggcagat tacaataccc tgtatggatg gctgttcaca ccgggcgtca
     1921 acctgcgtta caatttcacc gagaacctgg tattcagggc caacgcagga aggggttacc
     1981 gcactcctta cagtattacc gacaacattg gggttctgtc gaccggtcgc aggatagtgt
     2041 tcggagaaac accggaaatg gaagaagcct ggacctacgg attgaacctg acacttgtgc
     2101 tgcctttcgg tcacgggggg tcatccacgc tgagcctgga atatttccgc accgatttca
     2161 ttaaccagct cattgccgac caggaatacc tctggaacgc acaggtccct tccattcttt
     2221 tctataacct ggaaggccgc tcctatacca acacatggca ggctgatttc actaccgagc
     2281 cgttcgacgg attttctgtt ctggccactt ttcgctacaa tgattccaag gtgcaactga
     2341 aaggacaggg attggtggac cgccccctga ccagcaagtt caagggggtt ttgaaccttc
     2401 aatacaccac ccccagggac acctgggtct ttgatgtcac cgggcagata aacggtccgg
     2461 ccaggatacc ttcctttgca gcggatactg aagaaaagac ttcacctgtc tttcccatgt
     2521 tctatgcgca ggttacccgc aagctcaaaa acatggacat ttatattgga ggtgaaaacc
     2581 tgatgaacta caggcaaccg gacccgatca tctctgccga tgacccgttt tcaccccaat
     2641 tcaatgccag cataatctgg ggacccctca tgggtatcag ggtttacgcg ggcatgcggc
     2701 tatccctgtt caggtaagtc atatattggt ttatatttgc aggagaaaac tcattaattg
     2761 tagcaatatg acacgttcta tgacatgctg gcttatctgc ctgctatttt ttcagataat
     2821 gacggcacag gaaacggggg cctggatacg gatcaatcag gccggttacc tgcccggtga
     2881 catcaaggtt gccgtactga tctccaaaga agaggcttct cccgttgctt tccgtgtact
     2941 ggacatgaga acggacgcct gtgtcttttc cggttcggtc gaagaaggga ccattaaaga
     3001 agtacccgcg gtcaaatggg ggatggcctc ggcttttcgc ctggatttca gcgaattgaa
     3061 agaggagggc ggctaccgtg tcgtgacgga cattcccggg aagggaactg tggaatctcc
     3121 ggctttcaga ataggggccg aagtatatga gggaacggcc gatttcctgc tcacttacat
     3181 gcggcagcag cgttgcggcg acaatccctt tctggataca ctctgccatc agaacgacgg
     3241 gtacatagtg ctgcatcccg aacgcacggg agaaaagata gatgtcaggg gtggctggca
     3301 cgatgccaca gattacctgc aatacctcac caccagtgca aacgccacgt tccagatgat
     3361 gttcgcctac actcaggcag aggataaatc ggtgttcaaa gaccgctttg atgccacggg
     3421 caggccgggg gtaaacggga tccccgatat ccttgacgag gtccgctggg gtctggaatg
     3481 gctcttgaag atgaaccccg aagacaagat gatgttcaac cagatcgccg atgaccggga
     3541 ccatgtgggt ttccggcttc cggtgggaga caaggccgat tacgggtggg gacctggcgg
     3601 gggacgtccc gtctatttca ttaccgggga aaggcaggga ttaagggagc acatcaaccg
     3661 gacaaccggg gtggcctcct cggccgggaa atttgcctcg gcttttgccc tgggggcgga
     3721 attgttcagg gaactggacc ccggttttgc agaaaaaatg aaggccaagg cactgccggc
     3781 ttatcatttt gccgaggaaa aaccgggcaa tacgcagact tgttgcgtag tttctcccta
     3841 tttttacgaa gaagacaatt atgtggatga cgtagagttg gcagccgctg ttttccacca
     3901 cctgggggca ggggaggaat ggcttcaaaa ggccgattac tggggacagc tggaagaggt
     3961 gaccccgtgg atggagctgg gccgggcacg gcattaccag ttttatccct tcataaacct
     4021 gggacactat tacctggcct cgtcggatac gccaatggcc gaaaaatata cggaatacat
     4081 gcgtcgggga ctggagcata tccggcaaag gagcagtgac tgcgctttta tgaacgggat
     4141 tcccttaatg tggtgttcca gcaacatggt ggcagctgcc attacgcagg ccgacctgta
     4201 ttacaggctg accggggaca gtacttacag ggagatggaa gcttcgctgc gcgactggct
     4261 tttcgggtgc aatccctggg gaacttccat gatcgtggat tttccccgcg gtggcgatta
     4321 tcccaaacgg ccgcatactt catacctgcc caccctgggc aaaagcaccc cgggcgggtt
     4381 gattgatggc ccgcaactga gggaacgcct taaggaacac agccagtata tttccctggc
     4441 cgacggggcc gcctcttacg ctccgttcaa ccagggggtt gccctgtacc atgacgagac
     4501 gtgggattat gcaaccaacg agcctaccat ggacgggacg gcctcgctga cttattacct
     4561 ggccgggaga gaagctgagg gcagaaagga agctgccggt cttgcagcgg acaaatcacg
     4621 tccggcaacc gaaagagacc agcgcggggg catcatccgc atgaatcccg gaaccccgga
     4681 gatctacctg accttcacgg ccgacgagcg ttttgaaggg ggcaggaccg tgcttgaaac
     4741 attgaaaagg aacgggataa aagcttcttt ctttcttacg ggcaagtgcc tgcgggatcc
     4801 tcagtggaaa gaactggtgc aggacatcat aagccagggg cattatgtgg ggcctcacgg
     4861 agacatgcac cttcagtacg ctccctggga cggggacata gaacagtcgc ttgtttccgg
     4921 cgatagtctc aggaacgaca ttgcctgcaa tatggacgaa ctgtcgcgtg tcgggctgga
     4981 tgtctcgaaa atccgctggt tcatgccttc ctatgagcat ttcaatacag agacggtccg
     5041 ggtgactgca tcaatgggaa tggaagtgat ccacctgact cccggcatca tgacactggc
     5101 ggattacacc actcccgaca tgccatccta caggagcaca aaggaattac tgaagcagct
     5161 gtatgatctt gaaagagaaa aaggactgca cggggccatc atgctcatcc atccgggaac
     5221 agaaccctcc aggactgaaa aattgtatga ccggcttgac cagatcataa agaccctgaa
     5281 aagaaaggga tacgttttcc ggcgccttcc ctgatgctct ggacgaagct tttttatgta
     5341 tctttggccg ttttataatg attcccgttt atgtacaaaa tcctgaaaaa acgcatactg
     5401 gcacctgata tctgcctgat ggaagtgtcg gcgccccgtg tggcgtccag cgcacaaccc
     5461 ggacaattcc tgattgtcag ggtaggagaa aaaggagaaa ggattcccct taccatctgt
     5521 gatttcgatc ccgtggccgg ctcggtcacc attgtgacac aacgggtggg agcctcatca
     5581 cgcaagatct ttgcccttga agaaggggag tcttttaacg attttgcagg gccactgggc
     5641 gttccttccg aattcatcca tgaaagggaa gaaattctga aagaaaagaa atttcttttc
     5701 attgccggcg gtctgggtac cgccccggtg tacccccagg taaaatacct gcacggcaaa
     5761 ggagcccatg tggaagtgat cattggcgcc aaaacccgcg acctggtcat catggaagaa
     5821 gaaatgaaac aggtggccgc ccgggtacac atctgcaccg atgacggctc atacggattc
     5881 aaaggactgg ttacggccag gctcaaagaa ttgcttgatg gtggcgcacg gttcgatcag
     5941 gcagttgcca ttggccccat gatcatgatg aaattcgtaa ccctgacggc caggaaatac
     6001 aacctgccgc tcatagtcag cctgaacacc ctgatggtgg acgggacggg catgtgcggc
     6061 gcctgtcgtg tcacagtagg aggaaaaata cggtttgcct gcgtggatgg gcctgaattc
     6121 gacgcctacc aggtggattt tgacgaggcc atgaggcggc agtccatgta caaattacag
     6181 gaaaaggatg cagatcacaa atgtaaaatc ggtttaggca atggtcaata aaatcagggt
     6241 cccggtaagg gaacaggatc caaaagccag gaccgggaat ttccaggagg tcagttacgg
     6301 gtacaatgcc caagaagccg tgcttgaagc atcccggtgt atccagtgca agaatcccaa
     6361 atgtgtgacc ggctgtccgg taaccataca aatcccacaa ttcataaaag ccatcctgga
     6421 aggggacatg cccggggcag catccgtcat agcacgggat tcctccttgc ctgcagtctg
     6481 cggacgtgtc tgtccccagg aatcacagtg cgagggggtg tgtgtcatgg ggatcaagaa
     6541 cgatcccata gccattggga aactggaacg gtatgtggcc gactgggcac gggaaaataa
     6601 ttatacaacg gccataccgg ctgttcccaa cggcaggaaa gtggccatag tgggttcagg
     6661 accggcagga cttgcctgtg ctaccgacct ggccaaggcc ggttgtgacg ttacgatctt
     6721 tgaagccttg cacaaaccgg gcggcgtgct cgtttatggt ataccggagt tccgtctgcc
     6781 caaggcaaag gtggtggatt atgagatagg gcaggtcagg aaactgggtg tgaagattga
     6841 atgcaacgta gtggtgggtc ggaccgtcac catagatcaa ttatttgatc aagagggttt
     6901 tgatgcggtt tttgtgggat caggcgccgg actgccaaaa ttcctgggca ttccgggcga
     6961 gaatctcaac ggagtggtct cggccaatga attcctgacc aggaataacc tgatggaggc
     7021 atacgttccc gaatcggaca cgcccatcta cgtcggaaag cgcgtggcag ttgtcggtgg
     7081 cggcaatgtg gccatggatg ctgcaagaac ggctttgcgc ctcggggcgg aagtcgaagt
     7141 ggtttaccgc aggacccgga aagaaatgcc cgccagggtg gaagaagtgc atcacgccga
     7201 agaggaagga gtcacctttc aaatgctgac caacccggtt gccgtgttgg gaaatgaaaa
     7261 aggctgggtc accggcctca ggtgcatccg gatggaattg ggcgaacccg atgaaagcgg
     7321 caggcgcagg cctgttgagg tacccggatc cgaatttgac attgcctgcg atgtgaccgt
     7381 catagcgttg ggaacctctt ccaacccact gatcgcgcgt accactccgg gactggagat
     7441 gaaccggcac aacgggatcg ttgccgatga aagaggcatc acatcacgcc ccggtgtttt
     7501 tgcaggaggg gatgtagtaa ccggttccgc cacggtgata ctggctatgg gagccggccg
     7561 ccaggcggca aaatcaatcc tggaatacct ggaagcctga tctcttgctg catattgcgg
     7621 gaggaagacc ctatgcgcag ggtataatcc ccggcatcta ccacccagcc tgttcccgga
     7681 acataagaag cgagccgctc catgggaatg attatttcag ccatgcagga ttccccgggg
     7741 cccagcaggg gcgtcttggc aaaacccttc agttcctggt acggcttatc tcccatacct
     7801 tcaggtgcgc tgacatatgc ctggacgact tcacggccat ttttttcccc tgtattagtt
     7861 atcacacact gaacgacaat ccgttccggc tccacccgga catcaggctt ggcatatagg
     7921 aatgaagtat aggacagtcc gtacccgaag tgataggcca cctctttccg atggctgtcc
     7981 acatcacggt atcccacgaa aattccttcc tcatagcggg tgtagtctat gttgagcctt
     8041 tctgctgctg tttttttctc ttttttcccc atcatggaca gccacttcac cggtactcct
     8101 tccaggggaa aattcttgtg tgaggcaagg tcctccaggt atgccgggat ggtcaccggg
     8161 agccttccgc acggggtttt ctttcccgag acgatatcgg ccagggcccg gcctccttcc
     8221 tgtcccggca tccaggccag caacagggca tcgggcagac cggaccagga ggcggtttca
     8281 acaatgccac ccacattcag caccacgaca acgggtttcc cgactgcctg gaaagccttg
     8341 cagacattgc caataagtgc tttttcctct tccgagagca gataatcccc ttctattgtc
     8401 cggtccgtga attcccctgt cctgcggcct atggtgatga tggccacctg gttttcaacc
     8461 gcagagcggg cgatagcttc tgcacccgga tctgtctccg tgggaaggcc ctgtcccgtg
     8521 attctttggg tgatggtttt ccattttctt ttgtaagccc ggttgacact gtccgtgtag
     8581 ctttggtagc gctccctgag ctgtgggtca ggcaggtagc ctgcttcctg cagaccttcc
     8641 gccggagaaa cagtgtgatc tgcgtagact tctcccgaac caaaaccgcc tgcaaccagc
     8701 ctgtaggagg aaaccccaaa cagggcgatc ctgcggatgg agtcattgaa aggaagcgca
     8761 ttgcgatttt ccaggagtac catgccttct gcagcggcat cacgggctat tgccctgtaa
     8821 ccggcctgac aggcgctgtc ctgtatgcct gttccggaag gtgagtcttt ttgtgcctgt
     8881 ttcctgtat
//
LOCUS       DAOSOF010000079         8889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7834_length_8889_cov_4.068599, whole genome shotgun sequence.
ACCESSION   DAOSOF010000079 DAOSOF010000000
VERSION     DAOSOF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7834_length_8889_cov_4.068599"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..568)
                     /gene="trxA"
                     /locus_tag="PLM68_05595"
     CDS             complement(146..568)
                     /gene="trxA"
                     /locus_tag="PLM68_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT54872.1"
                     /translation="MLVLYAGNEGTADITKENKTKIMELTKDEFIKRVYNYEANPKEW
                     KYEGNKPCIVDFHATWCAPCKMLAPTLEELNQEYNGQIYIYKVDVDKSPELAAIFGVR
                     SVPTLLWIPMEGKPGVSQGALPKSQLESIIKNTLLKKE"
     gene            complement(684..944)
                     /locus_tag="PLM68_05600"
     CDS             complement(684..944)
                     /locus_tag="PLM68_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOT54873.1"
                     /translation="MTCSALLIAAGCKVQNPEFKVTIENTVKELDLDKDYTFALIGSK
                     KAKFLWILSRTPEMAQADLERVLNKARSLGYKVEYLIWVEHN"
     gene            1079..1273
                     /locus_tag="PLM68_05605"
     CDS             1079..1273
                     /locus_tag="PLM68_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54874.1"
                     /translation="MEQERVRAIIDRYRSRAQSAREASQIDKSREMEEFADFVEDNLD
                     AFLDEAYTMESELWEEYENY"
     gene            complement(1267..>1459)
                     /locus_tag="PLM68_05610"
     CDS             complement(1267..>1459)
                     /locus_tag="PLM68_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT54875.1"
                     /translation="KLRNYFLELTRDNKYGPFTMPHTHQELADIFGVTRPALSRAIRQ
                     LEDSGVVRKHGGRLYELLQ"
     assembly_gap    1460..1469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1470..1907)
                     /locus_tag="PLM68_05615"
     CDS             complement(<1470..1907)
                     /locus_tag="PLM68_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT54876.1"
                     /translation="MEGLYQSPLFEGLDTLTIDCLLDGACRLRNYVPGELVALQGDVY
                     KSLLIVDKGMLRTEMTDPEGTKVTLEEIAASRTVAPAFIFATNNCLPVSIFAAISSSV
                     VSIPRNKLKEIMHDNNRVLENFLGIISDRSRFLSERLRLLSFGT"
     gene            2003..2683
                     /locus_tag="PLM68_05620"
     CDS             2003..2683
                     /locus_tag="PLM68_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT54877.1"
                     /translation="MKRTVVRIDEKRCNGCGLCVTGCHEGALQIVGGKAVMVREDYCD
                     GLGACIPECPRGAISLEEREADPFDQKAVQEHMLQTVQQQQQPVRQQPVMFPVKAPQP
                     VQFPVQLHLINPQNTLFRGADLLLAAVCTAFTTPDFHTRYLKNKLLAIACPKLDSNCD
                     VYVEKLAVMIEETGIETLTVLIMEVPCCGGLLEIVKKAREKSSRYIPVKKIILTIQGN
                     LKSEEWIK"
     gene            2719..4338
                     /gene="hcp"
                     /locus_tag="PLM68_05625"
                     /gene_synonym="priS"
     CDS             2719..4338
                     /gene="hcp"
                     /locus_tag="PLM68_05625"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOT54878.1"
                     /translation="MSMFCFQCQETARNQGCTIKGVCGKEPQTAALMDLLIYVCRGIA
                     IVQRALRQNGKAVDAPDHFLMDALFTTITNANFDDAALSERINKGFALKKDLLAQARQ
                     EGINIPDMPEVRLTAAPADYQNVAATVGVLSEPDEDIRSLKQMTIYGLKGMCAYAEHA
                     LNLGHANVEIYTFLEEALYQVSRNDIGADELVALVLKTGETGVKSMALLDKANTSTYG
                     HPQMTRVNIGVGKNPGILISGHDLRDMEDLLEQTSGTGVDVYTHGEMLPAHYYPAFRK
                     YPHFVGNYGNAWWKQREEFESFNGVILFTTNCIVPLAKEGTYLDRMYTTGSCGYPGAK
                     HIPARKEGGKKDFSELITMAKTCKPPHEIEKGEIIGGFAHNQVLALADQVVEAVKTGA
                     IKRFVVMAGCDGRMKSRQYYTDYAMALPKDNVILTAGCAKYRYNKLPLGDINGIPRVL
                     DAGQCNDSYSLVLIALALKDAFGLKDVNDLPIFYNIAWYEQKAVIVLLGLLSLGVKNI
                     HLGPTLPGFLSPNVAKVLVEKFGIGPQPELN"
     gene            complement(4343..5068)
                     /gene="pssA"
                     /locus_tag="PLM68_05630"
     CDS             complement(4343..5068)
                     /gene="pssA"
                     /locus_tag="PLM68_05630"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOT54879.1"
                     /translation="MKRILLHIPNAITSLNLLCGALGIAFVFRDRQDIAFWLLITASV
                     FDFLDGFAARLLNAYSTVGKELDSLADAISFGLLPAFMLYAVYLPAAPRLWLTFLPLI
                     VAVFTAIRLAKFNVDDRQAESFLGLPSPASALLIASAVFYTTGNPSWKEFMQTTWFIP
                     TISVVISYLLISELPMFSLKFQGFSFGRNKVRYSFLVLCLVLVITLLATGNHPGLAMM
                     GCICLYMVMSGILRISGHHYHSS"
     gene            complement(5065..6144)
                     /locus_tag="PLM68_05635"
     CDS             complement(5065..6144)
                     /locus_tag="PLM68_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT54880.1"
                     /translation="MKKNLLISLAVLVLSGTLSAQQYEFRTVVDNPSTSLKDQARTGT
                     CWCFATISFLESELMRMGREPIDLSEMFIVRNVYKARYFDNYLRTGKGNLGPGSVSHT
                     ATKAIARYGLIPDAAYPGIQYDAPGHNHSELQGYINAISQVPARMKKMSPESDELLDA
                     LLDIYLGKVPQTFLYNGKEYTPKSFARYLGLNMDDYIEITSFTHHPFYTRIPVEIPDN
                     WEHELYYNVPLDEYMAIADHALNNGFTIAWDGTLTKNFSQQDGVATEPSPDPSADEVN
                     VTQDIRQEWFETFSTVDDHLMHVTGIAKDQDGVTYYITKNSWGESGKYKGFMYMSENY
                     FRARCIAYMVHKDAIPKDIRKKLGL"
     gene            complement(6159..7358)
                     /locus_tag="PLM68_05640"
     CDS             complement(6159..7358)
                     /locus_tag="PLM68_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT54881.1"
                     /translation="MKRILICIIALAAITLQATAQEQEKKNEEGFLFTVIKENPITPV
                     KNQSSTGTCWSFSSLGFMESELLRMGKSEYDLSEMFVVSRTYTDKADKYIRLDGALNF
                     AQGGGFDDYLYVLRKYGMVPDEVMPGLNYGEDVHRHGELTAVLTGIVEAVKKNPNRKL
                     STAWKNAFQGALDAYLGEIPETFEYKGTTYTPISFAASLGFNPDDYVNITSFTHHPFY
                     TQFALEIPDNWLWGTSYNVPMEEMMSVIDHAIMEGYTVLWASDVSEQGFGRNGIAMYP
                     TTDPREMIGSDQARWLEMSATERSNAFRNLKAPVAEIKEVTQEMRQEAYDNKQTTDDH
                     GMQIYGIAKDQNGTKYYMVKNSWGDDAGNYKGLWYVTEKFVQYKTMDIQVHKNSIPRD
                     IKKKLNL"
     gene            complement(7372..7992)
                     /locus_tag="PLM68_05645"
     CDS             complement(7372..7992)
                     /locus_tag="PLM68_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOT54882.1"
                     /translation="MNSLLDEGRLLPLAEDFYTIQGEGFHAGKAAYFIRLGGCDVGCS
                     WCDSKFTWNPRVFPPVPVTEIIARAAKYPARAVVITGGEPLTYPLDILCNGLKKEGIE
                     IFLETSGSRPLSGSMDWICLSPKQNHPPLPEFYPLANELKVIITGPLDMEWAEFNRRK
                     VNPHCHLYLQPEWSRREAVVPHIISYIKANPSWKISLQTHKFINIP"
     gene            8071..>8889
                     /locus_tag="PLM68_05650"
     CDS             8071..>8889
                     /locus_tag="PLM68_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOT54883.1"
                     /translation="MNEQKYTIQAQELLQSAAQIAQSGSNQVIETGHILKAMLSDREG
                     IGVYLMQKAGVNTENLENELDIILNKYPRQQGSTDSYLSRESAEALQKARDVANRMGD
                     QFITVEHILVGVSSGKDSVAKLLRSAGLNEKLLDKAIEGVRQGRKISSRTEEQTFDAL
                     NKYAINLNELSVKGKVDPVIGRDDEIRRVLQILSRRTKNNPILVGEPGVGKTAIAEGI
                     AQRIVTGDVPDMLKSKQIYSLDMGALIAGAKYKGEFEERLKAVVKEVITAEGEII"
BASE COUNT         2362 a         2019 c         2208 g         2290 t
ORIGIN      
        1 cagcgatccc tcaagaaccc atatgaattt gtacaggtct ttgttttgca gaagcgattc
       61 actgttgtga aaatcatcag tgactatgag actcccctgc agtggagtta tgcacgcata
      121 ttccatatgt gcaaagttaa gaaacctatt cttttttcag caatgtgttc ttgatgattg
      181 attcaagctg gcttttgggc agcgcgccct gtgaaacgcc gggtttaccc tccatgggta
      241 tccataacag ggtaggaacg cttcgaactc cgaaaatcgc tgccagttct ggtgatttat
      301 ccacatccac tttatagatg tagatctggc cattgtactc ctggttcagc tcttccagcg
      361 tgggagccaa cattttgcag ggagcgcacc aggttgcgtg gaagtccact atgcagggtt
      421 tgtttccctc atacttccat tctttgggat tggcttcgta attataaacg cgtttgatga
      481 attcgtcttt tgtgagttcc ataatctttg tcttgttttc ttttgttata tccgctgtcc
      541 cttcattgcc tgcataaagc accagcattg ggagcaatgc cagaaaagtt actatagttt
      601 tcatgaggca aagatcaggt ttaatcaaaa aacggacaat agctattttc cactatatgt
      661 tggcaaatct tcccgatatg aaattaattg tgttcaaccc agataagata ttcaaccttg
      721 tagccgagag aacgagcctt gttcagcacc ctttccagat ctgcctgggc catttccggt
      781 gtacgtgaaa gaatccacag gaattttgcc tttttgcttc ctatgagggc aaaagtataa
      841 tctttgtcca ggtccagttc cttgacagtg ttttctattg tgaccttaaa ttcagggttc
      901 tgcaccttgc atcctgcagc tatcagcaaa gcactgcagg tcaaaaaaaa tattcatctt
      961 tttcataaat aattgttttt ctaaactaaa aagaatgctc agagtggtac ggttatcttt
     1021 ggttgttgta aggacaacaa catgcaaata tatggatatt tttacgtaaa ttcagaccat
     1081 ggaacaagag agagttagag ctataataga tcgttacagg agtcgggcac agtcagccag
     1141 ggaagcttcc cagatagaca aatcccgtga aatggaagag tttgcagatt ttgtggaaga
     1201 taacctggat gcctttcttg acgaagctta taccatggaa tctgaactgt gggaagaata
     1261 cgaaaattat tgaagcagtt cgtaaaggcg acccccgtgt ttcctgacaa ctcctgaatc
     1321 ttccagttgc ctgatggcac gggatagggc aggtcttgta acaccaaaaa tatctgcaag
     1381 ttcctggtgg gtatggggca tggtaaaagg gccatactta ttgtcccggg tcaattccag
     1441 aaaataattc ctcagtttgn nnnnnnnnnc gtgccaaagc tgagcagccg gagtctttcc
     1501 gatagaaacc tgcttctgtc ggaaatgatg cccaggaaat tttcaaggac cctgttattg
     1561 tcatgcatga tttctttcag cttgtttctc gggatggata caacagaaga tgaaatagcc
     1621 gcaaaaatac tgacaggcag gcagttgttg gtcgcaaata taaaggctgg agctactgta
     1681 cgggatgcgg cgatctcttc aagggtgact ttggtacctt ccggatcggt catttctgtg
     1741 cgaagcatgc ccttgtctac gatgagcaac gatttataca catctccctg aagggccacc
     1801 aactctccgg gtacataatt gcgaagccgg caggcgccat ccagcaggca gtctatggta
     1861 agagtgtcaa gaccttcaaa aagcggagat tgatacaagc cttccataag gtacaaagtt
     1921 aggtaattcc cggttttgta acaaatgtta caaattgtag tgcaggcagc atcctatttt
     1981 tgcagtgtaa ttcgtgaatc atatgaaaag gacagtagtc aggattgatg aaaaacgttg
     2041 caacggctgt ggattatgtg tcaccggatg tcatgaggga gcactgcaga ttgttggcgg
     2101 gaaagctgtc atggtaaggg aagattactg tgacggattg ggggcgtgca ttcctgaatg
     2161 cccccggggg gccatttccc tggaagaaag agaagctgat ccttttgacc agaaagcagt
     2221 acaggagcat atgctgcaaa ctgtacaaca acagcagcaa cctgtcaggc aacaacctgt
     2281 aatgtttccc gttaaggcac cacaacctgt ccagttcccc gtccagttgc atttgatcaa
     2341 tccccaaaac acccttttca ggggagctga cttattgctg gctgccgttt gtacggcttt
     2401 caccactccg gatttccata cacgttacct gaagaacaaa cttctggcca tagcctgccc
     2461 caaacttgac tccaactgtg atgtttacgt ggaaaaactg gccgtgatga tagaagaaac
     2521 aggaatagaa acgttgacgg tcctgatcat ggaagttccc tgttgcgggg gactgctgga
     2581 gattgtaaaa aaagcccgcg aaaaaagctc ccgttatatt cccgtgaaaa aaatcatatt
     2641 gacgatacag ggcaacctga agtcggaaga atggatcaaa taaaaagact atatttatca
     2701 actattaaca aaaaaagtat gagtatgttt tgttttcaat gccaggaaac agcccgtaac
     2761 cagggttgta cgatcaaagg cgtctgtgga aaggagccgc aaacagcggc cctgatggat
     2821 ttgctgattt atgtctgcag ggggattgcc attgtacaga gggcccttcg tcaaaacggg
     2881 aaagccgtgg atgctcccga ccattttctg atggatgccc tttttaccac cataaccaat
     2941 gcaaatttcg atgatgctgc cttatctgaa cgcataaaca agggattcgc cctcaaaaaa
     3001 gacctcctgg cacaagcccg tcaggaagga atcaatattc ccgatatgcc tgaggtcaga
     3061 ttaacagctg cacctgctga ctaccagaac gtagcagcaa cggtgggagt tttgagtgaa
     3121 cccgacgagg atatccgttc cttaaagcaa atgaccatat acggtttgaa aggcatgtgt
     3181 gcctatgccg agcatgccct caacctggga catgcaaatg tggaaattta cacttttctg
     3241 gaagaagcgc tttaccaggt atcccgcaat gacatcggcg ctgatgaact tgtcgccctg
     3301 gtgctgaaaa ccggtgaaac cggggtaaaa tcaatggctt tgctggataa ggctaataca
     3361 tccacctatg gtcatcccca aatgacccgg gtaaatatcg gagtgggcaa gaatcccggt
     3421 atcctgatat ccggtcacga cctcagggat atggaggatt tgctcgaaca gacttccgga
     3481 accggtgtgg acgtgtatac ccatggagaa atgctccctg cccattacta tccggctttc
     3541 aggaagtatc ctcactttgt agggaactat ggcaatgcct ggtggaaaca acgtgaggaa
     3601 ttcgagtctt tcaacggagt gatccttttc accaccaact gcatagtacc tcttgcaaaa
     3661 gaaggtactt acctggaccg catgtataca accggttcgt gcggataccc gggagcaaag
     3721 cacattcctg cccgtaagga aggcgggaaa aaagattttt ccgaactgat aaccatggca
     3781 aaaacctgca agccccctca cgagatagaa aaaggtgaaa tcattggcgg atttgcacac
     3841 aaccaggtac ttgcccttgc agaccaggta gtggaagctg taaagacagg agccatcaaa
     3901 cgctttgtgg tgatggcagg atgcgacgga cgtatgaaat cacgtcagta ctacacggat
     3961 tacgctatgg ctcttcccaa ggacaacgtt atactcacag caggatgtgc caaatacagg
     4021 tacaacaagc tgcctttggg agatatcaat ggaattcccc gggttctgga tgccggacaa
     4081 tgcaacgaca gctattcact cgtactcatt gcacttgcct tgaaagacgc ttttggcctg
     4141 aaggatgtga atgacctgcc cattttctac aacatcgcct ggtatgagca gaaagctgtc
     4201 attgttctgc tagggctgtt gagcctggga gtgaagaata tccatctggg tcccacactg
     4261 cccggattcc tatccccgaa cgttgcaaaa gtgctggtcg agaaattcgg gattggtccc
     4321 caacccgaac tgaattagga ctttacgaac tatgatagtg atgtccggag atcctcagga
     4381 ttccggacat taccatatac aggcatatac accccatcat ggccagcccg gggtggttcc
     4441 cggttgccag cagggttatg accagaacaa gacaaaggac caggaaagaa tacctgactt
     4501 tgttcctgcc aaagctgaaa ccctggaact tgagggagaa cattggcagc tcgctgatga
     4561 gaagatatga gatgaccacc gatatggtgg gtatgaacca ggttgtttgc atgaattctt
     4621 tccaggaagg attgccggtt gtataaaata cggccgatgc tatgagcaat gcgcttgccg
     4681 ggctgggaag gcccaggaaa ctttcggcct ggcggtcgtc aacattgaac ttagccaggc
     4741 gtatggccgt aaaaacggcc acgatcaggg gtaagaatgt aagccacaag cgaggcgctg
     4801 ccggcaggta tacggcataa agcataaagg ccggcaacag gccaaagctg atggcatcgg
     4861 ccagggagtc cagttctttg cctactgtgg agtaagcatt gagcaaccgg gcggcaaaac
     4921 cgtccaggaa atcaaaaaca gaagccgtta tcaacagcca gaaggctatg tcttgcctgt
     4981 ccctgaaaac aaaggcaatt cccaatgcac cgcacagcag gttcagcgat gtgattgcat
     5041 tgggaatatg caacaggatt cttttcaaag tccgagtttt ttgcgaatat ccttgggtat
     5101 ggcgtcttta tggaccatat aggcgatgca cctggcgcgg aaataattct cagacatata
     5161 catgaatcct ttatactttc cggactctcc ccaagaattc ttggtgatgt agtaagtaac
     5221 gccgtcctgg tctttggcaa tcccggtgac gtgcatcagg tggtcatcga ccgtactgaa
     5281 ggtttcgaac cactcctggc gaatatcctg ggtgacgttc acttcatctg ccgaaggatc
     5341 gggcgaaggc tctgtggcca ccccgtcctg ttgtgaaaaa tttttagtaa gggtgccgtc
     5401 ccatgcaatg gtgaatccat tgttcagggc atgatcggct atggccatgt attcatccag
     5461 cgggacattg taatacagtt catgttccca gttatccgga atctccacgg gtatccgggt
     5521 gtagaaaggg tggtgggtga aactggtgat ttcaatataa tcatccatgt tcagccccag
     5581 gtatcgggca aaagattttg gggtatactc tttaccattg taaaggaagg tttgcggaac
     5641 cttgccgagg taaatgtcca ggagcgcatc gagcaattcg tccgattcag ggctcatttt
     5701 tttcattctt gccggaacct gggaaatggc attgatatat ccctgtaatt cggaatggtt
     5761 gtgtccagga gcgtcatatt gaatgccggg ataagccgca tcgggtatca atccgtagcg
     5821 tgcaatggct ttggtcgccg tatggctgac agaacccggg cccaggttac ccttacccgt
     5881 tctcagataa ttgtcaaagt aacgtgcttt ataaacgttt cggacaatga acatttcgga
     5941 caagtctatg ggctcacggc ccatgcgcat caactcgctt tccagaaagg aaattgttgc
     6001 aaaacaccag caggtgcctg tacgggcctg gtctttcagt gatgtggacg ggttgtcaac
     6061 gaccgtgcgg aattcgtact gctgagcgct taaagtgcct gaaagcacca gtactgccag
     6121 tgagatcagt aggttcttct tcatgatgta taacgttgtt ataagttcag tttcttttta
     6181 atatccctgg gaatggaatt cttatgcacc tggatgtcca tggtcttgta ttgcacgaat
     6241 ttttccgtca cgtaccacag ccccttatag ttgccggcat catcacccca ggaatttttc
     6301 accatgtagt attttgttcc gttctggtct ttggcaatcc catagatctg catgccgtgg
     6361 tcatctgtcg tctgtttgtt gtcataggct tcctgtctca tttcctgcgt gacttctttg
     6421 atctctgcca cgggagcttt caggttcctg aaggcgttac tcctttctgt ggcggacatt
     6481 tccagccagc gggcctggtc cgagccaatc atttccctgg gatcggtcgt ggggtacatg
     6541 gctatcccgt tccttccgaa gccttgctcg gaaacgtccg aggcccagag caccgtatag
     6601 ccttccatga tggcatggtc tatcacagac atcatctctt ccatgggaac gttgtaggaa
     6661 gtgccccaaa gccagttgtc gggtatttcc agggcaaact gtgtatagaa aggatgatgg
     6721 gtgaacgaag tgatattgac ataatcgtcg ggattgaatc ccagcgatgc ggcaaatgat
     6781 ataggtgtgt atgtggttcc cttgtattcg aatgtttccg gaatttctcc aaggtaggca
     6841 tccagcgctc cctggaaggc gtttttccag gcggtggaga gtttgcggtt gggattcttt
     6901 ttcactgctt caacaattcc tgtaagaacg gctgtgagtt cgccatgccg gtgaacgtct
     6961 tcaccgtaat tgagccctgg cattacctca tcgggaacca tcccgtattt gcgcaacaca
     7021 tacaggtaat catcaaatcc gcccccctgg gcaaagttca gggctccgtc cagacgtatg
     7081 tatttgtcgg ccttgtctgt atatgttctt gatacaacaa acatttctga gagatcgtat
     7141 tcgctctttc ccattctaag gagttcagat tccatgaaac ccaatgaaga aaagctccag
     7201 caggtgccag tactgctctg gttctttaca ggtgtgatgg gattctcttt tataacggta
     7261 aacaggaatc cttcttcgtt tttcttttcc tgttcctgtg cggttgcctg gagggtgatt
     7321 gccgcaaggg cgattatgca gatcaagatt cgtttcataa aaaagtgtag tttaaggaat
     7381 gttaatgaat ttatgggttt gtagtgatat tttccaggat ggattggctt tgatgtatga
     7441 aataatgtgt ggcaccacag cttccctccg gctccattcc ggctgcaggt aaaggtggca
     7501 atgcgggttg actttccttc tgttgaactc ggcccactcc atatcgagcg gcccggtaat
     7561 gatcacttta agctcattgg ccaacggata aaattccgga aggggagggt ggttttgttt
     7621 gggtgaaaga catatccaat ccatgcttcc gctcaggggc cgggacccgg atgtctccag
     7681 gaatatttca ataccctcct ttttaagtcc attacaaagt atatccaggg ggtaggtaag
     7741 gggttctccc ccggtgatca ccacagcccg ggcaggatat ttagctgccc tggcaatgat
     7801 ctctgtaaca ggaacaggag gaaataccct gggattccag gtgaatttgg agtcgcacca
     7861 ggaacatccc acgtcacaac cgccaagcct gataaaatag gcagcttttc ctgcgtgaaa
     7921 accctctccc tgaatggtat aaaaatcttc ggccagcggt agcaggcgcc cttcatccaa
     7981 tagcgagttc attccacaaa tgtaaagaaa tattgtgaca aattgtcaga aaattcactt
     8041 tggaacaaga tttgagcctt atacggtact atgaatgaac agaaatatac tatccaggcg
     8101 caggagctgc tacagtcagc cgctcagata gcgcagtccg gttccaacca ggtgattgaa
     8161 acgggacata tattgaaggc gatgctttcc gacagggaag gtataggggt ataccttatg
     8221 caaaaagcag gcgtgaacac agaaaatctt gaaaatgagc ttgatataat actgaataaa
     8281 taccccaggc agcaaggttc gacagactct tacctgtcaa gagaatctgc tgaagccctg
     8341 caaaaggcca gggatgttgc caacaggatg ggggaccagt tcattaccgt tgaacacatc
     8401 ctggttggag tgagctcagg taaggacagc gtggcaaaat tactgcgctc tgccggactg
     8461 aatgaaaaac tccttgataa ggccatagag ggagtacggc aggggagaaa gatatcttcc
     8521 cggacagagg aacagacatt tgatgccctg aacaaatacg ctattaatct taacgaattg
     8581 tctgtcaaag ggaaagtgga tcccgtgatt gggcgtgatg acgagatacg cagggtgctt
     8641 cagatcctta gcagacgcac taaaaacaat cccatactgg taggcgagcc gggagtcggc
     8701 aaaaccgcta ttgccgaggg catcgcccag cgaattgtga ccggagacgt ccccgatatg
     8761 ttgaaaagca aacaaatcta ttccctggat atgggtgccc tgatagccgg ggcaaaatac
     8821 aagggtgagt ttgaagaacg cctcaaggct gtggtaaagg aagtgatcac ggcagagggc
     8881 gagatcatc
//
LOCUS       DAOSOF010000080         8826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7907_length_8826_cov_6.537111, whole genome shotgun sequence.
ACCESSION   DAOSOF010000080 DAOSOF010000000
VERSION     DAOSOF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7907_length_8826_cov_6.537111"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3222
                     /locus_tag="PLM68_05655"
     CDS             <1..3222
                     /locus_tag="PLM68_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOT54884.1"
                     /translation="ARARREDFARTFSGRSHLSRLNGVASATPASLRSISSGNRKFLT
                     ILVQFSDKSFTSASPQGDFFNLLNQDGYAVNGATGSAWNYFRENSSDTFDPQFDVVGP
                     VTLSHDMAYYGANVTDEDDNEDDIRPREMVVEAVDLADQLGVDFSQYDNDGDGYIDNI
                     FIYYAGYNEAEGGPDDSVWPHAWSIYNQRTVDGVKTGSYACSSELRGNSGSTMAGIGT
                     FCHEFGHVIGLPDFYDTDYEDNGEARGLGSFALMSHGNYNNEGRTPPYLNSMERDLLG
                     WFDNDPGVIDEEGDYSLGPVTGNVCYVSETANEGEIFLYEYRKQTGWDAYIPSGLLIY
                     HIDRSDNMVDGKTAAARWNDWDGINAYASHQCFDLVEAVYPESAVQNNAQVPFPGSTN
                     NTSFTGASSPAAVDYAGNRTGTDLTDIANTGNGAIFTVIKSNQLVINGVVADPEGNPV
                     AGATVTLSYQAPDDISRAAATGWNTPDRGNIKASPKRSMEQVSEVTTNAQGAYTFTTG
                     TAEGVYIITVSKQDYHTAEKEISGINPGTAQLNFTLYPIVQITGDVLRKHNAWEGRSA
                     GFGDPGGTIYGAVGFSAEELVLCQGKRIKTVSFQLSGTTAAEAGIFVTMGSDVVLNCK
                     VDNPDFGNMMTVDVSADELIIPEGQLVKIGCYVKDSDSGFPIAIDEGPMLPMGGYAAN
                     AIASLTEPWSLDYNILVSATLEEVIIPEATLTGSVTDDEGVAIAGVTVSLYYDNATST
                     APVNAPRLSKEPGQLRKSPLRAGTLITTAVTDQEGAYTFTTETRKTGSFILQTSKTGY
                     YQAMRQVPASQSGDITVDFTLIPVVDAGNGILKKHGLWSTNIGSGTPGGTIYGAVGFT
                     AQEIAPYEGFVVNTISCLVNGTSAGEVGVFAYLDQECVLTQVVSNPSFGTMMQVDVSA
                     HELVIPAGKPVYFGYYVKESDAGYPLTADDGPMVSMGGHARFTMGAFSTDWKEQYDLD
                     HNILVSATVGPRDNELFSQGYFMIKHPAKTYREGDLFRFELNESTLVEGVTRPSAVQW
                     YFDGTAYSATDVITLTAGSHSIMVVLTFAGYSQTIVKEILIGE"
     gene            3284..4951
                     /locus_tag="PLM68_05660"
     CDS             3284..4951
                     /locus_tag="PLM68_05660"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT54885.1"
                     /translation="MKTDIEISHEYQLLDIRDVAANAGIACENLILYGNHMAKVPAGQ
                     IDEDKVKESNLILVTAITPTRAGVGKTTVSIGLSMGLNHIGKKAMVALREPSLGPCFG
                     MKGGAAGGGYAQVVPMEDINLHFTGDFHAVTAANNMISALLENHLYNNQNNGTCLREI
                     VWKRVLDVNDRTLRAIISGVGGKANGITRATGFDITPASEIMAILCLSKDIDDLKHRI
                     GQIILGYGHDGSPFYVNDMGVAGAITILLKDAIHPNLVQTLAGTPAFVHGGPFANIAH
                     GCNSLVATKMAMSHADYVVTEAGFGADLGAEKFFNIKCRKGGLQPKLTVLVATLQGLK
                     MHGGQDLARLTEKNPESVIRGLANLEKHVANLRTFGQSIVVALNHYESDAEEEISLVR
                     DRCRELDVAFAVDDAYAQGARGAAALASAVVEVIESKPSRPLVFTYSDDDPIEQKIWK
                     VAHNIYGARDVFYYETARESLEKIRELGLEHYPVCIAKTQFSFSTDPKAYGVAHDFEM
                     NIHDLIINNGAGMIVAIAGDILRMPGLPKKPQAVFMDIKDGVITGLS"
     gene            complement(4959..5777)
                     /locus_tag="PLM68_05665"
     CDS             complement(4959..5777)
                     /locus_tag="PLM68_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOT54886.1"
                     /translation="MKKNAALSLLLLIVLNSCGTTAFTGRKQLMVFSDESISALSDES
                     YAEFLKTAVISGDKQHSDMVRSVGEKMVTALTSYLSGIGQQDYLGGLGWEFQLVKDPS
                     VNAFCLPNGKIVLFEGLLQFAGNPNHMAIVIGHEMGHAIARHGNERMSQQALLQTAGQ
                     IATQVFGQTKYGQNAQAAELFAQAFAFGTNVGVVLPFSRKHEYEADRIGLYIMALAGY
                     DIYQTPLFWEKMMGDKKSSSFDFLNTHPADIKRIAAISESLDEAAKYYRDPEFK"
     gene            5848..7089
                     /locus_tag="PLM68_05670"
     CDS             5848..7089
                     /locus_tag="PLM68_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOT54887.1"
                     /translation="MKQLFLFLVTAVAFISLPINAEGQNSVAAGTVKATGLKDSSPRP
                     RMRDYGVRTGVMQPGPLNAITDVKGVSVGHVTLVAGDSIRTGVTAIIPHPGNIFREKV
                     PAAFWAGNGFGKLAGSTQIEELGNIETPVILTNTLSVAAGIEGLVTYTLQQPGNGDIQ
                     SVNAVVGETNDGGLNDIRGRHVTPAHVLEALVKAAPGPVAEGNVGAGTGTICFGFKGG
                     IGTASRILPKSLGGYTVGALVQSNFGGVLQIAGVNAGERLGRYSYKSQIEEQSGGIRV
                     GHPAPDGSCMIIIITDAPLNERNLERMAKRGMMGLARTGGIAANGSGDYVIALSVAPG
                     NLIREYASGTKPYSPTYTPTYLHNNECSALFMAAIEAVEEAILNSLFAARTMTGKDGR
                     TVEALPVEKVVKLFPLVKDVE"
     gene            7152..7661
                     /locus_tag="PLM68_05675"
     CDS             7152..7661
                     /locus_tag="PLM68_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT54888.1"
                     /translation="MNTRITLFLLFTMLTAVGFAKDRNSQQTKQNPEEEQSMKEVHDF
                     LRTCGVYYIATVDIDQPRVRPFGTAVIFEQKLYIQTGRKKNVARQMMANPKVEICAYD
                     ASKGTWLRIEAKVVPDERLEAKQYILDQYPQLKSMYKAEDENTLVLYLKDATATFNSF
                     SSPARTVKF"
     gene            complement(7936..>8826)
                     /locus_tag="PLM68_05680"
     CDS             complement(7936..>8826)
                     /locus_tag="PLM68_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54889.1"
                     /translation="MFRVPEDYYQGINRACLAAYHHDTGLLAYSFMSNHFHVIAETGD
                     PYGYIGSIRNGYNKWFNNKYSRKGPLGYAGFHAVELDTLEHILEGTTYVLRNGVHHNV
                     VSYPSGYPFCSTRYYFMKDFGVHGQYTPIISWKEQREFLPWKVKLPPGYLIDKSGLLI
                     QENILQTKMVEHYYGSPRKFSYYLNKLSGEEWAMLNIESWMGNKIKDLLNNEKLIGKV
                     NRISDVDVCLFIDQWLKNNKNGAPYTTLQHEEKLSLALLLRQKMADPGQIARCLAISR
                     DVPENLKNAHDLGRSRKCPS"
BASE COUNT         2256 a         2124 c         2360 g         2086 t
ORIGIN      
        1 gccagggcaa gaagggagga ttttgccagg acattttccg gcaggagcca tttgtcccga
       61 ttgaatgggg tggcttcagc cacaccggct tccctgcggt ccatttcttc cgggaacagg
      121 aagttcctga ccatcctggt gcagttcagc gataagtcct ttacctcggc aagcccccag
      181 ggtgactttt tcaatttgct gaaccaggac ggctatgccg tgaacggggc gacaggttct
      241 gcctggaatt atttccggga aaattcttcc gatacttttg accctcagtt tgacgtggtc
      301 ggacccgtta ccttgtctca tgacatggct tattatgggg caaatgtcac agatgaagat
      361 gataatgaag atgatatccg gcccagggaa atggtggtcg aggcagttga cctggcagac
      421 caactggggg tggatttttc gcaatacgac aacgacggtg acggatacat agataatatt
      481 ttcatctatt atgcgggata caacgaagct gaaggtggtc cggatgattc cgtctggccc
      541 catgcctgga gcatatacaa tcaaagaacc gtagacgggg tcaaaaccgg gagttatgcc
      601 tgttcatcgg aattaagagg aaattccgga agcaccatgg ccggtattgg gaccttttgt
      661 catgaattcg ggcacgttat aggattgccc gatttttatg acactgacta tgaagataat
      721 ggagaagcaa ggggcctggg atcctttgcc ctgatgtccc atggcaatta taataatgaa
      781 ggcagaacgc cgccttacct gaactccatg gaacgggacc tgctgggctg gtttgacaat
      841 gatccggggg tgattgacga ggaaggggat tattcattag ggccggtgac gggaaacgtt
      901 tgttacgtga gtgaaaccgc caatgaagga gagatttttc tttatgaata caggaaacaa
      961 acaggatggg atgcttatat accttcaggg ctgctcattt atcacataga caggtctgat
     1021 aacatggtgg acgggaaaac ggcagccgcc cggtggaacg actgggatgg catcaatgct
     1081 tatgcctctc accagtgttt cgacctggtt gaagccgtat atcccgaatc tgccgtacaa
     1141 aacaacgccc aggttccatt cccgggcagt acgaacaata cttctttcac aggggcctcc
     1201 tccccggcag ccgtggatta tgccgggaac cgtaccggga cagacctgac tgacattgcc
     1261 aatacaggaa acggggccat atttactgtg attaaaagta atcaattggt aatcaatggc
     1321 gtggtcgccg atcctgaagg aaatcctgtg gcgggtgcca cagtgaccct ttcatatcag
     1381 gcaccggacg atatttccag ggcggctgca acgggttgga acacaccaga caggggcaat
     1441 atcaaagcct cgccaaaacg gtctatggag caggtgagcg aagtgacaac caatgcccag
     1501 ggggcgtata ctttcacaac cgggaccgct gagggcgtgt atatcatcac ggtcagcaaa
     1561 caggattatc atacggcaga aaaggaaatc agcggaataa acccgggaac cgctcagctg
     1621 aatttcaccc tgtatccgat agtacagatc acaggagatg tccttagaaa gcacaatgcc
     1681 tgggaaggcc gtagcgcagg tttcggagat ccggggggaa ccatttacgg ggctgtaggg
     1741 ttttcggcag aagaactggt actatgccag gggaaaagga taaagaccgt atctttccag
     1801 ctgagcggca ccacggcagc agaagcgggt atttttgtta ccatgggttc ggatgtggtg
     1861 cttaactgca aggtggacaa tcctgacttt gggaacatga tgacggttga tgtgagtgca
     1921 gatgagctga ttattccgga ggggcaactg gtgaagatag gatgttatgt caaagatagc
     1981 gatagtggtt ttcccattgc catagatgaa ggtcccatgc ttccgatggg cggatatgcc
     2041 gctaatgcca ttgcatccct gactgaaccc tggtcacttg attacaacat cctggtatcg
     2101 gccacgctgg aagaagttat catcccggaa gcaacgctta ccggaagcgt gacagatgat
     2161 gagggtgtgg ccattgccgg ggtaaccgtg tccctgtatt acgacaacgc aacttcgact
     2221 gctccggtca atgcgccgag actgtcaaag gagcccggac agctgagaaa atcgcctctc
     2281 agggccggta cattgatcac tacagcggtg acagaccagg agggagcata tacctttacc
     2341 acggagacca ggaagacagg atcctttatc ttgcagactt ctaaaacagg gtattatcag
     2401 gcaatgcggc aggttccggc cagccagtca ggagatatca cagttgattt cacgctaatt
     2461 cctgtggtgg atgccggcaa cgggatactg aaaaagcacg ggctttggag cacgaatatc
     2521 ggatcgggca cacccggagg aaccatttat ggagcggtgg gtttcacagc acaggaaata
     2581 gccccctatg agggttttgt ggtcaacacc atatcatgcc tggtaaacgg gacatcggcc
     2641 ggggaagtgg gtgtttttgc ctacttagac caggaatgtg ttttgaccca ggtagtcagc
     2701 aacccgtctt tcgggacaat gatgcaagtg gatgtaagtg cgcacgagct ggtcattccg
     2761 gccgggaaac cggtttattt cggttattat gtcaaagaga gtgacgccgg atatcctctg
     2821 acggccgatg acgggcccat ggtatccatg ggcggacatg cacgctttac catgggagca
     2881 ttttccaccg actggaaaga acagtacgac ctggaccaca acatcctggt ttcagcaaca
     2941 gtgggaccca gggataacga gctcttttca cagggatatt tcatgatcaa acaccccgct
     3001 aaaacatata gggaaggaga tctcttcagg tttgaactga acgagtccac cctagtggaa
     3061 ggggtgacaa ggccctctgc cgtacagtgg tatttcgatg gcacggctta cagtgctaca
     3121 gatgtcatta ccctgaccgc aggcagtcat agtatcatgg tggtccttac ctttgcaggc
     3181 tactcacaga cgattgtaaa ggaaatcctt ataggcgaat aaccgtcacc cgctgttcct
     3241 gcatcccccg gaattttgta tttttgggaa aaaccggtgt gatatgaaaa cagatattga
     3301 aatttcccat gaataccagt tgctggatat cagagatgtg gcggccaatg ccgggattgc
     3361 ctgtgagaat ctgatcctct acggaaatca tatggccaag gttcctgccg gccaaattga
     3421 tgaagacaag gtaaaagaga gcaatctgat cctggtgaca gccattacac caaccagggc
     3481 aggcgttgga aagacaaccg tttccattgg cttgtccatg gggctgaacc atatcggcaa
     3541 aaaggcaatg gttgcgctgc gcgaaccttc cctgggtcct tgtttcggga tgaagggcgg
     3601 ggcggccgga ggcggttatg cacaggttgt tcccatggaa gacatcaacc tgcactttac
     3661 cggggatttc cacgcggtaa cagcggccaa caatatgatc tcggctttgc ttgaaaatca
     3721 tttgtacaat aaccagaaca acggtacatg cctgagggag attgtctgga aaagagtgct
     3781 ggatgtgaat gaccgtaccc tgcgggcgat catctctggg gttgggggca aggccaatgg
     3841 gatcacccgg gctacgggct ttgacataac gcccgcttct gagatcatgg ccatcctctg
     3901 cctttccaaa gacattgacg atctgaagca caggataggg cagatcatcc ttggctatgg
     3961 ccacgacgga tccccattct atgtgaacga tatgggtgtc gcaggggcca taaccatctt
     4021 gctaaaagac gccatccacc ccaacctggt gcaaacgctc gcgggtaccc cggctttcgt
     4081 gcacggaggt cccttcgcca atattgccca tggctgtaat tcactggtag caaccaagat
     4141 ggccatgtca cacgccgatt atgtggtgac cgaggccggt tttggagccg atctgggtgc
     4201 cgagaaattt ttcaacatca aatgccggaa agggggcctg caacccaaac tgaccgtttt
     4261 ggtggccaca ttgcagggac tgaaaatgca cggaggacag gaccttgcca gattgactga
     4321 aaagaacccg gaaagcgtga tcaggggatt ggccaacctg gagaaacatg tcgccaacct
     4381 cagaaccttt ggtcaaagca ttgtggttgc ccttaaccat tatgaatccg atgcggaaga
     4441 agagatctcc ctggtgcggg accgttgtcg cgagttggat gtggcatttg cggtggacga
     4501 tgcctatgcg cagggagccc gtggagcggc ggcactggcc tcggctgtgg tggaggttat
     4561 tgaaagcaaa ccatcaaggc cgctggtatt cacatactcc gacgatgatc ccattgaaca
     4621 gaagatctgg aaagttgcac acaacattta cggtgcgcgc gacgtgtttt attatgaaac
     4681 ggccagggag agcctggaaa agatacggga gcttgggctg gagcactatc ctgtctgcat
     4741 tgccaagaca caattctctt tctctacaga tcccaaagct tacggggttg cacacgattt
     4801 cgaaatgaac atacatgacc tgatcatcaa taacggagcc ggtatgatcg tggccattgc
     4861 cggggatatt cttcgcatgc caggccttcc caaaaaaccc caggccgtgt tcatggatat
     4921 caaagacggc gtgataacgg gtttaagtta agaaggcctt atttgaattc cggatcccgg
     4981 tagtattttg ccgcctcgtc aaggctttcg gatatggctg caattctttt aatatctgcc
     5041 ggatgtgtgt tcaggaaatc aaaagaggag gacttcttgt cacccatcat tttttcccag
     5101 aacaaggggg tctgataaat gtcgtaacca gccagggcca taatatacag gcctatccgg
     5161 tctgcttcgt attcgtgttt gcgggagaag ggcaaaacca cacccacatt ggttccgaac
     5221 gcaaatgcct gggcaaacag ttccgctgcc tgcgcgtttt gtccatattt tgtctggccg
     5281 aacacctgtg tggcgatctg tcctgcagtc tgtaacaatg cctgctggct catgcgttcg
     5341 ttcccgtgcc ttgcgatggc atgccccatc tcatgcccga tgactatggc catgtggttg
     5401 ggattaccgg caaactggag gagcccttca aagagtacga tcttgccgtt tggcaggcag
     5461 aaagcgttaa cggaaggatc ctttaccagc tggaactccc agcccaatcc gccaagataa
     5521 tcctgttgcc cgatcccgga caggtatgat gtaagcgctg taaccatttt ttctcccact
     5581 gagcgaacca tgtcactgtg ttgcttatct cccgagatca ccgctgtctt gagaaattcg
     5641 gcataagact catccgacag ggcagagatg gattcgtctg aaaataccat caactgcttc
     5701 cggcctgtaa aggcagttgt accgcaggaa ttgagcacaa tcagcaggag taacgaaagt
     5761 gctgcattct ttttcatttg taaaatagtt tggtgtgtct caaatataat gattttttaa
     5821 ctttgtctgt ataccaaaaa tctggtcatg aagcaattat tcttattttt agttaccgct
     5881 gtcgccttta tatcccttcc tataaacgct gaagggcaaa attccgttgc ggccggcacg
     5941 gtaaaagcta ccggcttgaa ggattcatcc ccgcgtccaa ggatgcggga ttacggggtc
     6001 cggaccgggg tcatgcagcc cggaccgctg aatgccatta ccgatgtgaa aggagtgtct
     6061 gttggtcatg tcacgcttgt ggcgggagac agtatccgta ccggggtgac tgccatcatc
     6121 ccgcatccgg ggaatatatt ccgggagaaa gtgcctgccg ctttctgggc cggcaacggg
     6181 ttcggcaaac tggccggttc cacgcaaata gaggaattgg ggaacatcga gaccccggtc
     6241 atcctgacga acaccctgtc ggtggctgcc gggattgaag gattggtaac ctatacgctg
     6301 caacagccag ggaatgggga catacagtct gtcaatgccg tggtagggga aactaacgac
     6361 ggcggattga atgatatacg gggccgccat gtaacacccg cacacgtcct ggaggctttg
     6421 gtaaaagctg ccccgggacc cgtggccgag ggaaatgtgg gtgccggaac gggaaccatc
     6481 tgctttggct tcaagggagg catcggcaca gccagccgca tcctgcccaa aagcctgggt
     6541 ggttataccg ttggagcgct ggtccagtcc aatttcggag gtgtgctgca gattgccgga
     6601 gtgaacgcgg gggagcgcct cggccggtat tcctataaaa gccagattga ggaacagtca
     6661 gggggaatta gggttggtca tcctgccccg gacggttcct gcatgattat cattatcaca
     6721 gatgcgcccc tgaacgagcg gaatctggaa cgcatggcaa aacgcggcat gatgggattg
     6781 gccaggaccg gggggattgc cgccaacggc agcggtgatt atgtcatagc gctgtctgtt
     6841 gccccgggga acctgatccg ggaatatgcc tctggcacta agccctactc acccacctat
     6901 acacccacct acctgcataa caatgaatgc tcggccctgt tcatggctgc gatagaagcg
     6961 gtggaagaag ccatcctcaa ctcgctcttt gccgcaagaa ccatgacagg caaggatggc
     7021 cgcacggtgg aagccctccc ggtggaaaaa gtagtgaagt tatttcccct tgtcaaggat
     7081 gtggagtagg ggccgctgaa accatggaga aacaaaacat gaaattatta acattaagta
     7141 ataaacacat catgaacaca cgaatcacct tgttcctgct gtttaccatg ctgaccgcag
     7201 tgggttttgc caaagacagg aattcacaac aaacaaaaca gaatcctgag gaagaacaat
     7261 ccatgaaaga agtccatgat ttcttaagga cgtgcggggt atattacatt gctactgttg
     7321 acattgacca gccccgggta cgtccttttg gaactgccgt catattcgaa cagaaacttt
     7381 acatacagac gggccggaaa aagaatgtgg ccaggcagat gatggccaat cccaaagtgg
     7441 agatctgcgc ttatgacgca tccaagggaa catggctgcg catagaagcc aaggtggtac
     7501 cggatgaacg cctggaggca aagcaatata tcctggacca gtatccgcaa ttgaaaagca
     7561 tgtataaggc cgaggacgaa aacaccctgg tcctttacct gaaagacgcc acggccacct
     7621 tcaacagctt ttccagcccc gcccggaccg taaagttctg agcggtcttt gttgcttgtg
     7681 cgctcatact cccatgagga tagggagtac tttctatgca ccgcccttga agaccaacag
     7741 gcgatccttt gagcatcaat actcatttat ggcaaggtcg ctgcaccaca aagcacaggg
     7801 agcttctttg gtacgccgag gctccctgtg ctttgtggtg tcagcgaccg ctctcctcat
     7861 caaaaagatt tcactgtgca aacaggagga acctcaggga gagtttacca ctctgatccc
     7921 ggcatgagaa cgagattatg atgggcattt ccgggatcgt cccaggtcgt gagcattctt
     7981 gaggttttca ggaacatctc tggaaattgc caggcaacgg gctatctgac cagggtctgc
     8041 cattttctgc ctgagtaata gagccaggct gagcttttct tcatgttgga gagtagtgta
     8101 aggagctccg tttttattgt tcttcaacca ctgatcaata aacagacaca catcaacgtc
     8161 gctgattctg ttgacttttc ctataagttt ctcattattc aacaagtctt ttattttatt
     8221 gcccatccat gattcaatat tcagcatagc ccattcctct ccggatagtt tgttcaagta
     8281 atacgaaaat tttcttggag atccgtaata gtgctctacc attttagtct gaagaatatt
     8341 ttcctgtatt aacaatccgc ttttatctat tagatagcca ggtggcagtt ttactttcca
     8401 gggaagaaat tcccgttgct ctttccatga gattattggg gtatattggc catgaacccc
     8461 gaaatccttc atgaagtaat atcttgtgga acagaaagga tatccgcttg gatacgacac
     8521 gacattatgg tggactccat ttcgtaatac gtaggttgta ccctccagaa tatgttccag
     8581 tgtgtccagc tccactgcat ggaaaccggc ataacccaaa ggccctttac gcgagtattt
     8641 attgttgaac catttattat agccattccg tatggacccg atatatccat aaggatcccc
     8701 ggtttcagca ataacatgaa aatggtttga catgaatgag tatgccaaca ggccggtatc
     8761 atggtgatag gccgccaggc aagccctgtt tataccctgg taataatctt ccggaacccg
     8821 gaacat
//
LOCUS       DAOSOF010000081         8783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_7962_length_8783_cov_5.174038, whole genome shotgun sequence.
ACCESSION   DAOSOF010000081 DAOSOF010000000
VERSION     DAOSOF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_8783_cov_5.174038"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLM68_05685"
     CDS             <1..687
                     /locus_tag="PLM68_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54890.1"
                     /translation="VVRDAGIPTPDFCLVRSINELDKQKPDFPLFLKPVMEGSGKGIG
                     EHSLVWTEEEFQAVCCHLLKRYEQPVLVETYLPGREFTVGICGNGEDTRVIGVMEVVF
                     KKDILEIYSYENKQNYRETIEYINQTGEIAEKCARLALRVWEATGSRDSGRVDMKLNE
                     MGEPEFMEINPLAGLNFKDSDLPIIARMNGMNYTQLIGAIMQAAMKRIYGSEAQLVMM
                     QDEQYSYYPA"
     gene            659..1627
                     /locus_tag="PLM68_05690"
     CDS             659..1627
                     /locus_tag="PLM68_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54891.1"
                     /translation="MSNTAIILHNRLSESPTPDEQDVMDQAELVRNALETLGYKCLVR
                     DVGTDLYQDLLPLADIKPAFVFNLVESVLGCTGLLHIVPSILQAWKIPYTGVGEDGLY
                     LTTRKTLAKKIMQEHGIRTPAWFSTAGASLLDKKRKYIVKPIAEEGSAGLDEQHVFDA
                     SLPGIPGWLGNFDPRGHFIEEYIEGREFNLSITGKPGGYTLYPIPEMIFRDYPPGKAR
                     ILGYRSKWEEGSFEYKHTTRQFDTLENDPQLTARLRDTAVACGMVFNLSGYFRVDIRV
                     GQDGVPSVLEVNANPCIAPDSGFIAAGLHAGFSQAQIIRQIINCLN"
     gene            1639..2133
                     /locus_tag="PLM68_05695"
     CDS             1639..2133
                     /locus_tag="PLM68_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54892.1"
                     /translation="MAFTFRNALRAGDLEELTFIMESTGLFYDYEIQVALEVLGAFLE
                     RGKASGYCCLVAEHDNHCVGYAVYGPTPCTRSGWDIYWMAVSKDFQGKGLGSEMMIRI
                     ENMIARQGGTQIWIETSGRQDYVPTRGFYKKHDYVMMAELADFYAPGDSKVIYGKKLY
                     LYKD"
     gene            2156..2719
                     /locus_tag="PLM68_05700"
     CDS             2156..2719
                     /locus_tag="PLM68_05700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT54893.1"
                     /translation="MEIKARKSIAVLVGSLRKESLNRKLAGVLIELAPDSLDLEIVEI
                     GNLPLYNEDLDQDPPEEWTAFREKIIKADGLLLVTPEYNRSTSAALKNAIDVGSRPYM
                     KNVWNNKPAAVISLSPGAIGGFGANHHLRQSLVCVNASVMAQPEMYIGGAGNLFDKNG
                     KLIDSSMEELIHKFFRSYNRWLSHFIE"
     gene            complement(2699..3466)
                     /locus_tag="PLM68_05705"
     CDS             complement(2699..3466)
                     /locus_tag="PLM68_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT54894.1"
                     /translation="MKKIYELGIAFGGGGTRGYYHAGVLKRLLELGYKPQIVSGTSVG
                     SIVAAMYAAGLSVESMQAAFSALALKDFLEPRLPKQYLTDSKPLRKILENTLKAKTFE
                     ELECPLKIIATCLESSKEVIFEKGPLIDAIIASCSVPVVFPPVKIEGKHYVDGGVIRN
                     VPVSVIREECKRVFAVNLIPSSGKDMAYNGSMKHIAERCVTMMFNAAAMYDLNLADLV
                     IEDVEMAGYSTYDLKHRDEMFNLGYNANAFKLLDKMG"
     gene            complement(3478..5952)
                     /locus_tag="PLM68_05710"
     CDS             complement(3478..5952)
                     /locus_tag="PLM68_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54895.1"
                     /translation="MKYLSTLLVCLILGTGNLSAARNTSAIRGIVVDKETRSPLFYAT
                     LAVRDSSNSVITATTAGSDGTFTLEGIPYGTYQLIATFIGYKEQSKKIELTSEQTDVG
                     VIEMEEDVQTLETAVITARVPVIEQKIDKIVMNVAEAVSTEGSNALEVLRKAPGVTID
                     MDGNVKLNGQAVSIWIDGRPSYLSGLELEALLRSTDGTTIDKIELMAHPSARYDAEGS
                     GGIINIKMKKNLLKGFSGSANGYYGGMKHKSYEQEAGGGLNMNLRTDKTNTMLNYSGR
                     YDNIGVQLDTRTIIGNATSFEQISHSDFLISSQSHTVKLANDFFLNKKNTVGFIVTTM
                     FRDQSQDNFGDDNLTITKLNGNLLSSMKSIIRTNSDYDNITTNVNYTGVFNEDKAQEL
                     TFNADYAYYDIMSGNFQDNSIVSGIGINEMFRQNSQQYINLVSGRLDYQQSFWKTGML
                     EAGLKWAMTQTNNDLFREDSLSGGWVPNTSMSNEFIYTEHIAAAYASVGRMLGNKWTL
                     KLGLRGEYTYALGDWITTKKVTTKNYFNLFPTVFVGYNPSANWRLNLSYTSRIGRPSF
                     SQLNPFRNYIDANSYVEGNPDLDSQLSRQMALSVTFKNHYNLALIYAHTRNLIMQDPY
                     YDETGTKILLWENFGNQYMLGGSLSVSELPVTEWLYFTLSGVCVYMGNTSNPTNGQNE
                     QYINNGVLSSVQGQLTFLLPKDWKIELMGVYQGKVPYGYFTVKPIFMMNGGVKKNFMN
                     NKATLSINVNDIFGTFDSNLVYKSEDNVDYSIDQSINLQKIRLSLSIRFGQGKAARAR
                     KVGDLEEASRVDTSGGISTGTTGTLP"
     gene            complement(5949..6308)
                     /locus_tag="PLM68_05715"
     CDS             complement(5949..6308)
                     /locus_tag="PLM68_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT54896.1"
                     /translation="MEFKENQAIYLQIAESLCERILSGKWKAEDRLPSVRELGATLGV
                     NPNTVARTYDYLQQREIIYNKRGIGYFVAPGAKQVILTTLRERFLLEELPLFFRRIDL
                     LSIPLTDIQDNYNKQKK"
     gene            complement(6314..7120)
                     /locus_tag="PLM68_05720"
     CDS             complement(6314..7120)
                     /locus_tag="PLM68_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54897.1"
                     /translation="MSNLFNIKRFYAFVCKEFRELPGKYGLTILSMIGGYLVLLLLLM
                     MLGISVSPFTRMTVIMVYVMITIIFAPSKLYGNVNHARKGLCYAMLPVSALEKTISMF
                     VINFLCTSILIVAGLFAADMLLYLIVPSRMEGFLLNYETARLFGEELIELFFLQSIFI
                     LGNIVFKRQKVTRTFISLIGIGFLLGLVMLLVFRAIGLENIERLADSILAEFPKEINN
                     WNILSYSTFNSTYRHIPLIRNIIIIAYSVTGLITAACWVGVYRLIKTTKY"
     gene            complement(7117..7953)
                     /locus_tag="PLM68_05725"
     CDS             complement(7117..7953)
                     /locus_tag="PLM68_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54898.1"
                     /translation="MIQINNLSFSYSKNNVFRNLSLSLSKGNIYGLLGQNGVGKTTLL
                     KLLSGLLRSEEESCMVNGYHPYKRQPEFLKDVYYLPEEPACPHGTVARYAKNNGSFYP
                     SFDKDLFYEFLERFEVQPEKRFRDLSAGQQKKAYVSYALALNTKILLLDEPSNGMDIP
                     SKTVLRSIVAGQINEERFIIISTHQVRDLENLIDPIIILDRKDVLLHASIEEIAQKVC
                     FINAATKQDDALYSEEIPIGYSLVKANTTGIDTKVNLETLFNAVLMHKEWFKEHFNTT
                     IL"
     gene            8074..8781
                     /locus_tag="PLM68_05730"
     CDS             8074..8781
                     /locus_tag="PLM68_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT54899.1"
                     /translation="MSIVRFSGVDVIKDTNLILQGVDLTLEKGDFIYLVGRVGTGKTS
                     LIKTLIGEFPLKKGEAEVAGFNLVKLKRRQIPYLRRKIGVVFQDFQLLQDRSAYDNLE
                     FVLRSTGWKNKKEMQQRIEQALDSVGMLHKKHKMPHQLSGGEQQRVAIGRAILNNPEI
                     ILADEPTGNLDSDTQQEIMQLFMEIHEKQQPAMLVVTHNLNLLQRYPGRIFKCEAGKC
                     SQVADRREIDLSGFMES"
BASE COUNT         2378 a         2008 c         2039 g         2358 t
ORIGIN      
        1 gtcgtgagag atgcgggaat acctactccc gatttttgcc tggttcgcag tattaatgag
       61 ctggataagc aaaaacccga ttttcccctt tttcttaaac ccgtcatgga gggctcaggc
      121 aaggggatag gagaacattc actggtatgg acagaagaag aattccaggc tgtgtgctgc
      181 catcttctga aacgttatga acaacctgtt ctggttgaaa catacctccc gggcagggaa
      241 ttcacagtgg gaatatgcgg caacggggaa gacacccgtg tcattggtgt catggaagta
      301 gtcttcaaga aagacatatt ggagatctac tcttatgaaa acaagcagaa ttacagggaa
      361 acaattgaat acataaatca gacaggagag attgccgaga aatgtgcccg tcttgccctg
      421 cgggtgtggg aagctacagg ctcccgtgac agcggccggg tagatatgaa actgaacgag
      481 atgggcgaac cggaattcat ggagatcaac ccgctggccg gcctgaattt caaagattca
      541 gaccttccca tcatagccag gatgaacgga atgaattaca cacagttgat aggagccatt
      601 atgcaggctg ccatgaaacg aatttacgga agcgaagcac agctagttat gatgcaagat
      661 gagcaataca gctattatcc tgcataaccg gctatccgaa agcccaaccc ctgacgaaca
      721 ggatgttatg gaccaggcgg aactggtcag aaatgcactt gaaaccctgg gttacaagtg
      781 cctggttcgc gatgtgggaa cagacctgta ccaggatctt ctgccgcttg ctgacatcaa
      841 gcccgctttt gtgttcaacc tggtggaatc ggtgcttggg tgtaccggtt tgttgcatat
      901 agtgccctct atcttgcaag cctggaagat accttataca ggagtaggag aagatggcct
      961 gtacctgacc acgaggaaaa ccctggcaaa aaaaatcatg caggagcacg gtataaggac
     1021 accggcctgg ttctccactg ccggggcaag cctgctggat aagaaaagaa aatacattgt
     1081 caagccaatc gctgaagaag gttctgcagg gcttgacgaa caacatgtat ttgatgcatc
     1141 acttcccggg atcccgggct ggctcggtaa ttttgatccg cgcgggcact ttattgaaga
     1201 gtatatcgag ggacgcgagt ttaacctgag cattaccgga aagcccggag ggtacactct
     1261 ctaccccata cccgagatga tattcaggga ttatccacca ggaaaggcca ggatacttgg
     1321 ataccgctcc aaatgggagg aaggctcctt cgaatacaag cacacgacac ggcaattcga
     1381 taccctggaa aacgatcccc aactaactgc caggttaagg gatacggcag ttgcctgtgg
     1441 aatggttttt aatctttccg gctatttcag ggtagatatt cgggttggcc aggatggtgt
     1501 tccgtctgta ttggaagtga atgccaaccc ctgcatagca ccagatagcg gatttattgc
     1561 agcaggacta catgcaggat tctcccaggc acagatcatt cggcaaataa ttaattgcct
     1621 caattagcat tacatagtat ggcatttact tttagaaacg ccttacgcgc cggtgacctg
     1681 gaggagttga ccttcattat ggaatctacc ggattgtttt acgactatga aatacaggtg
     1741 gccctggagg ttcttggagc gttcctggaa cggggcaaag cttcaggata ttgctgcctg
     1801 gtcgcagagc atgacaacca ttgtgtggga tatgccgttt acggacccac tccgtgcacc
     1861 cgttccggat gggatattta ttggatggcc gttagcaagg attttcaggg aaaaggattg
     1921 ggatctgaaa tgatgatccg gattgaaaat atgattgcca ggcagggagg cacccaaata
     1981 tggatagaaa cttcgggaag acaggattac gtccctacca ggggttttta taagaaacat
     2041 gattatgtga tgatggccga attggcggat ttctatgcac ccggggacag caaggttatt
     2101 tacgggaaaa aattatattt gtataaagat tgactcacaa ctttataaca atattatgga
     2161 aatcaaagca agaaagagca tagccgtcct cgtaggcagc ctgaggaaag aatccctgaa
     2221 caggaaactg gctggcgtat tgattgaact ggccccagac tctcttgacc ttgagatagt
     2281 ggaaataggg aacctgcccc tgtacaacga agacctggac caggatccgc ctgaagaatg
     2341 gacggctttc agggaaaaaa ttatcaaagc agacggactg ctgctggtca cccctgaata
     2401 caaccggtct acatcggccg cgctgaaaaa tgccattgat gtgggatcaa gaccttatat
     2461 gaaaaatgtc tggaacaaca aacctgcggc agttatttcc ctttcaccgg gagcaatagg
     2521 gggatttgga gccaatcacc acctgaggca atctcttgtc tgcgtcaatg cctctgttat
     2581 ggcccagccc gaaatgtaca taggcggcgc agggaacctg tttgataaaa acggaaaact
     2641 tatcgatagt tccatggagg aacttatcca taaatttttc cgttcctaca accgctggtt
     2701 atcccatttt atcgagtagt ttaaatgcat tggcattgta ccccagattg aacatctcat
     2761 ccctgtgttt caggtcatag gtgctgtacc ctgccatttc tacatcttct atcaccaggt
     2821 ctgcaagatt cagatcgtac atggccgctg cgttgaacat catggtcacg caacgctctg
     2881 ctatgtgttt catagatccg ttgtaagcca tgtcttttcc ggaagaagga atcaggttta
     2941 cagcaaaaac ccgtttgcac tcctccctaa tgacagacac agggacattc cttatcactc
     3001 ctccgtccac ataatgttta ccttcaatct ttacaggggg gaaaacgacg ggaacgctgc
     3061 atgaggcaat aatggcatct atgagcgggc ctttttcaaa aattacttct ttagacgatt
     3121 ccaggcaggt ggcaatgatt ttcagcggac attccagttc ctcaaatgtc tttgccttca
     3181 gtgtgttttc caggattttc ctcaagggtt ttgaatcggt cagatattgc ttgggtagcc
     3241 tgggttccag aaaatccttc aatgcaagtg cactgaacgc agcctgcatg ctttctacgg
     3301 aaagaccggc ggcatacatg gccgccacaa tggatcccac gctggttcct gaaacaattt
     3361 ggggtttgta tcccaattcc aacaggcgtt tgagaacccc tgcatgataa tagcctctgg
     3421 tgccgccacc accaaaggct attccaagtt catatatctt tttcatctac aatcctatta
     3481 gggcaatgtt cccgtcgtac cggtagatat tccccctgaa gtatctacac gggaggcctc
     3541 ttccaggtcc cctactttcc tggctcgggc tgctttcccc tgtccaaacc ggatactcag
     3601 gctcaaacgg attttctgaa gatttatact ctgatctatc gagtagtcaa cgttatcttc
     3661 cgatttgtac accaggttgc tatcaaaggt accaaaaata tcgttcacat tgatgctaag
     3721 cgttgccttg ttgttcatga aattcttctt gactcctccg ttcatcataa aaatgggctt
     3781 gactgtgaaa tagccatagg ggaccttgcc ctggtatact cccataagct ctatcttcca
     3841 gtcttttggc aacaggaatg tcagttgccc ctgcacactg ctcagtaccc cattgttaat
     3901 atattgttcg ttctgtccat tcgtgggatt agaggtattg cccatataga cacaaacccc
     3961 gctaagggtg aagtataacc attcggtcac gggcaattca ctcacagaca atgagccgcc
     4021 caacatatac tggttaccga aattttccca tagcagtatt ttggtaccgg tctcatcgta
     4081 atatggatcc tgcatgatca ggttccttgt atgggcatag ataagagcca ggttataatg
     4141 gttcttgaat gtgacactca gtgccatctg cctggacaac tgggaatcca ggtcgggatt
     4201 tccttcaaca tacgaattgg catcgatata gttgcggaaa ggattcagct ggctgaaaga
     4261 aggcctgcct atacggctgg tataagagag attaaggcgc caatttgcag atgggttgta
     4321 tccgacaaaa acagtgggaa atagattgaa ataattcttg gttgtcacct tctttgtcgt
     4381 tatccagtca cccagggcat atgtgtattc accccgtaat cccagcttta gcgtccattt
     4441 gtttcccagc atcctgccca ccgatgcata agctgctgca atatgttctg tatatatgaa
     4501 ttcattggac atgctggtat tggggaccca gccgcctgac agcgaatctt cccggaacag
     4561 gtcattgttt gtctgggtca tggcccactt aaggccggct tcgagcatcc cggttttcca
     4621 gaacgattgc tggtaatcca gacgccctga aacaaggttg atgtattgct ggctgttctg
     4681 gcggaacatc tcattgatac ctatgccgga aacaatgctg ttgtcctgga aattgcccga
     4741 catgatatca taataggcgt agtcggcgtt gaaagttagt tcctgtgcct tatcctcatt
     4801 gaaaaccccg gtataattga cgttggttgt gatgttatca taatcggaat tcgtcctgat
     4861 gatgcttttc atgctgctta gcagattgcc gttgagtttg gttatggtca ggttgtcatc
     4921 cccgaaatta tcctgtgact ggtcacggaa catggtggtg actatgaaac caaccgtgtt
     4981 ctttttgttc agaaaaaaat cgttggccag cttcaccgtg tggctttgtg aacttatcag
     5041 aaaatcggaa tgggatatct gttcaaaact agtcgcgttc ccaatgatgg tgcgagtatc
     5101 cagctggact cctatgttat cgtatcggcc gctgtaattg agcatggtgt tggtcttgtc
     5161 tgtgcgcagg ttcatgttca gccctccacc ggcttcctgc tcatagctct tgtgcttcat
     5221 ccctccatag taaccattgg ccgatccact gaatcccttg agcaggtttt tcttcatctt
     5281 tatattgatg atccctccgc ttccttccgc atcatatcgg gccgaggggt gggccatgag
     5341 ctcaatttta tcaatggtgg tgccatctgt ggaacgaagg agtgcctcga gttccagccc
     5401 gctcaggtag gatggacgtc cgtctatcca gatactgacc gcctggccgt tgagtttcac
     5461 attcccgtcc atgtcaatgg tcactccagg ggccttgcgc aatacttcaa gggcattgct
     5521 cccttccgtg gaaaccgctt cagccacatt catgacaatc ttgtcgatct tttgctcaat
     5581 gaccggaact cttgctgtga taacggcggt ttcaagggtc tgcacatctt cttccatctc
     5641 tatgacgcca acatcggtct gttcggaagt taactcaatc ttttttgatt gttccttgta
     5701 tccgataaaa gtagcaataa gctggtatgt tccgtaaggt attccttcca gcgtaaaagt
     5761 accgtccgat ccggctgtcg tagccgtaat gactgaatta gagctgtccc tgacggccag
     5821 cgttgcatag aaaagcggac tgcgtgtttc cttgtcaaca acaatccccc tgatagctga
     5881 tgtgttgcgg gcagcagaaa gatttcctgt tcccaagata agacacacca gaagcgttga
     5941 aagatatttc attttttttg tttgttgtaa ttgtcctgaa tatcggttaa aggtatggaa
     6001 agtaaatcaa tccttcgaaa gaacaatgga agttcttcca gcaaaaaacg ttccctgagg
     6061 gttgtaagga tcacctgttt cgcccccggc gcaacaaaat agccaatgcc tcttttgttg
     6121 tatatgattt ccctttgctg caaatagtca taggttctgg cgaccgtatt gggattaact
     6181 cccagggttg ctccaagttc cctgaccgac ggcaacctgt cctctgcctt ccacttcccg
     6241 ctcaggattc tctcgcacaa agattcggca atttgcagat atatggcctg attttcttta
     6301 aattccataa gaatcaatat tttgttgttt taataaggcg ataaacacct acccagcacg
     6361 cggcagtgat caatccagta acagaatagg caataatgat tatgttcctg ataaggggaa
     6421 tatgcctgta cgtgctattg aaagtactat agctcagtat gttccagttg ttaatttcct
     6481 tgggaaactc agccagaatg gagtcggcaa gcctttcaat gttttcaagc cctatggcgc
     6541 ggaaaacaag aagcatgacc aggccaagaa ggaagccaat ccctataagg cttataaacg
     6601 ttcttgtcac tttctgtctt ttgaaaacta tattgccaag gatgaaaatc gattgtaaaa
     6661 agaacaactc aataagctct tctccaaaaa ggcgcgctgt ctcatagttc agcagaaatc
     6721 cctccatacg ggaaggaaca ataaggtaca ggagcatatc ggctgcaaaa aggccagcca
     6781 caattagtat tgaagtgcag agaaaattaa tgacaaacat gctgatggtt ttctccaggg
     6841 cagaaacagg cagcattgca taacacaatc cttttcttgc gtggtttacg tttccgtaca
     6901 gtttggacgg ggcaaaaatg atggttatca taacgtatac cattatcacg gtcatccttg
     6961 taaaaggtga aacagagatt ccgagcatca tcagcagcag aagcaaaacc aaataccccc
     7021 ctatcatact taaaatggtc aatccgtatt ttcccggcaa ttcccggaat tccttacaga
     7081 caaatgcgta aaagcgtttt atattaaaca ggttgctcat aatattgtcg tgttaaaatg
     7141 ttccttaaac cattctttat gcattaaaac ggcattaaac aaagtctcca gattgacttt
     7201 tgtgtcaatg ccggttgtgt tagcctttac cagggaataa ccaatcggga tttcttcact
     7261 atagagcgcg tcatcctgct tagtggcagc attgatgaaa catacttttt gggcaatttc
     7321 ctcaatggat gcatgcaaca ggacatcctt gcggtcgaga attatgatag gatctatcag
     7381 gttttcaaga tcccggacct ggtgcgttga aatgataata aacctttctt cattgatctg
     7441 tcctgcaacg atactgcgta ataccgtctt tgaaggaatg tccatgccat tgctgggttc
     7501 gtccaaaagc aggatcttgg tattgagcgc caatgcataa gaaacataag cttttttctg
     7561 ctgccctgca gacaggtctc tgaatctttt ttccggttgc acctcaaaac gctccagaaa
     7621 ttcataaaaa aggtctttgt caaaagacgg gtagaaagac ccgttgttct ttgcataccg
     7681 ggcaaccgtg ccatgcgggc aggcaggttc ttcgggtaga taataaacat ccttaaggaa
     7741 ctccggctgc cgcttgtatg gatgataacc gttcaccata caggattcct cttcgcttct
     7801 gagcaaaccc gacaataatt tcagcaaggt ggtcttgcct acgccatttt gtcccaacaa
     7861 gccgtaaata ttacctttag acaatgacag ggatagattc cggaagacgt tattctttga
     7921 ataactgaag gatagattgt tgatttgaat cattgtttag tgtcttagtt cattagtaca
     7981 ctgcaaatgt aaacaccttt ttctattttc caaaaattat gtcacttttt taacaaatgt
     8041 gcccttttgt tatatttgta taaaataaga aatatgtcta ttgtaaggtt ttcaggggta
     8101 gatgtgatta aggacaccaa cctgatcctg cagggagtgg atcttaccct tgaaaaagga
     8161 gactttattt acctggtagg acgggtcgga acaggaaaga catccctgat caaaacactg
     8221 ataggcgaat tcccactgaa aaagggagaa gctgaagtgg ccggtttcaa cctggtgaaa
     8281 ttgaaaagaa gacaaatacc ctacctaaga agaaaaatag gtgttgtatt tcaggacttc
     8341 caactgttac aggaccgctc tgcctatgac aacctggaat ttgtcctccg gtctaccgga
     8401 tggaaaaata aaaaggagat gcagcagcgt attgaacagg ctctggatag cgtgggaatg
     8461 ctgcataaaa aacacaagat gccgcaccag ctctccggcg gggaacagca gcgcgttgct
     8521 attggccgcg ccatactcaa caatccggag atcatactgg cagatgaacc taccgggaac
     8581 ctggacagtg acactcagca ggaaatcatg cagctgttca tggaaattca tgaaaaacag
     8641 caacctgcca tgttggtggt cacacacaat ctgaacctgc tgcagcgtta cccgggccgg
     8701 atattcaaat gcgaggcagg aaaatgcagc caggttgcgg accgccgtga aatagacttg
     8761 tcaggattta tggaatcatg aaa
//
LOCUS       DAOSOF010000082         8544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8220_length_8544_cov_4.112734, whole genome shotgun sequence.
ACCESSION   DAOSOF010000082 DAOSOF010000000
VERSION     DAOSOF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_8544_cov_4.112734"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLM68_05735"
     CDS             <1..496
                     /locus_tag="PLM68_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT54900.1"
                     /translation="EGFRWSLERDYTYMIEMDADFSHNPEDLPRLVDACRQSADLAIG
                     SRYCKGISVINWPLGRVLMSWFASAFVRTILNMPIYDCTAGFICYHRRVLEGIDFRWV
                     TMKGYGFQISMKYYAYKLGFKIIEIPIIFVDRTEGTSKMSSGIFGEAFWGVLKLRFKR
                     IRKK"
     gene            500..1849
                     /locus_tag="PLM68_05740"
     CDS             500..1849
                     /locus_tag="PLM68_05740"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT54901.1"
                     /translation="MARNILIRNASIVNEGSVFRGCVVLKDQHIAGIFRGHKAEEEAV
                     AFCGTRDIDIDASGKIMIPGIIDDQVHFREPGQPGKGTMMQESRSALLGGVTSVMDMP
                     NNVPPVITAELLEQKYILAREKMYVNYAFYMGATNTNTREVLKPVRQSCGLKIFMGSS
                     TGNMLTDNHSVLNDLFCNYKGVIATHCEDESMIRENLASMKARYGDCIPLNLHSSIRS
                     REACIASTRKALDLALKYHSRLHILHISTKEEVELIREARCVHSGISCEASLPHMWFT
                     EEDYGRYGTQVKCNPAIKTAEDRDAILKALEKRDIQAIGSDHAPHTWEEKQQNYTNAP
                     SGIPLIQHTCQMMMELVQSRQLSLTSFVDACCHQPSILFGIRNRGFIREGYYADLVLI
                     DPEKEQQITKSQLEYTCRWSPLEGYIFSTSVSHVFINGMLAAENGQIIQKPPVLPIL"
     gene            1851..2780
                     /locus_tag="PLM68_05745"
     CDS             1851..2780
                     /locus_tag="PLM68_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT54902.1"
                     /translation="MTTQRIRAYLYICTTTLLWGVSFFWTNKLLINHVPVFFLILTRM
                     SLAALILLIVGRITGKIQRIKNKKDALWFLALALMEPFFYFIGETYGVKLTASPTISS
                     MIIATIPVFGLVAGLSFYKEKVTAGNIAGILMTVPGMLIVVSGRGGLETGNVTDLPGT
                     NMPLGAMFLFMAVFSAVGHTVILKRMTRSYNAFTVTFYQHALGALYFILPVLLIDAQR
                     INVKHFITKADFWYPILMLSVFCSSIAFMMFTAAIKELGVTRTNTFSAVIPIVTASVG
                     FALGIETINWIQALGILVVVFGLVLSQLRSRRS"
     gene            complement(2756..4912)
                     /locus_tag="PLM68_05750"
     CDS             complement(2756..4912)
                     /locus_tag="PLM68_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOT54903.1"
                     /translation="MNKNHIELIASELHIKDWQVEHAVTLFQDGATIPFVSRYRKEAT
                     GSLDEVQLAAVKSLWNSYEELDKRKSAILRSIEDQEKLTPELRAVIENCWQMNKLEDL
                     YLPYRPKRKTRASIARSKGLEPLAQALLGLPEQNCLEMAQGYLGEEVESVEDALSGAR
                     DIIAETVSENATLRQKVRYIYQRSGVLASMVQKGKEEEGIKYRNYFDYSENISSMAPH
                     RLLALLRANNEGIVSLGLKPHPDTMPLAEMEKMFMGNRKGSSTMHGPSSSVWQMEQAL
                     SDAYRRLIHPSIENEVLNYYKDKADRESIKVFSENLRQLLLAPPLGQKRVMAIDPGFR
                     TGCKVVCLSAQGDLLHNDTIYPHPPQNERATAMRKVSQMTEAYKIEVIAVGNGTAGRE
                     TEQFLKKIAFPHKIQIFSVSEDGASVYSASQAGREEFPDYDVTVRGAVSIGRRLMDPL
                     AELVKIDPKSLGIGQYQHDVNQTLLKESLDQTVEFCVNHVGVNLNTASLRLLTYVSGL
                     GPGLARNIVEYRQENGPFSSRQTLKKVSRLGEKAFEQCAGFLRIPGATDILDNTGVHP
                     ESYPLVRRMAADLGVTVEQLIEKEELRRKIDLSVYITPVTGIPTLRDIMTELDKQGRD
                     PRAVIKVWEFDESISSIEDLREGMVLPGIVSNVTNFGAFVNIGIKQDGLVHVSQMGEK
                     YVHDPLQVVKLHQHVMVKVLKIDLERSRIQLRLERS"
     gene            complement(4905..6662)
                     /locus_tag="PLM68_05755"
     CDS             complement(4905..6662)
                     /locus_tag="PLM68_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT54904.1"
                     /translation="MPKKIWTKIKSLPEPTLLLLLSLVIGLLSGLAAVILKSSIAFIK
                     DLLTTHVSVQAESLAYFLLPGTGMLLSLLFVRYFVKDNISHGVTRVLESISVSKSRIK
                     PHNCYTSIISSALTIGFGGSVGAEAPIVYTGAAIGSNVGRSLGLNYRSVTLLVCCGTA
                     AAIAGIFKAPLAGVLFCFEILLFNLTIGSLIPLLTASITATVVSSLIMGDGVSFASST
                     APFQITNIPYYLILGIVCGFTSIFFTRTTLMLESRIKRIHNIYTRWAFSALILGVLIL
                     LFPPLYGEGYGSLSALLNNNVEGAVDSLIYGPLSRNPWFIPIFFTAVLLLKVFAMSFT
                     NAGGGVGGTFGPTLFMGGILGFVISRALNLAGVSGLPESNFALVGMAGLMAGVMKAPM
                     TAIFLIAEITGGYQLLVPLIITAVCSFVTARGFEPYSIYTKRIAREGKLLTHDSDQAV
                     LTLLKTSDLIENDFMTVNPHDSLEHLVHVVEQSSRNLFPVTDLEGCLQGVVSLDDIRP
                     IMFRRELYGNVFVYELMHRPVEYVFIDERMESVMEKFERTDAWNLPVLDEKFRYMGFV
                     SKSRILSAYREQLSQVSHE"
     gene            complement(6634..6999)
                     /locus_tag="PLM68_05760"
     CDS             complement(6634..6999)
                     /locus_tag="PLM68_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54905.1"
                     /translation="MKKTTEVKPAINKKRLVIGYANMSPQLMDIWKEKYPRGYIDYME
                     DIMKVDKADGSFFYAVTLEIPDAIYLVKVEVKIDDYDEIEKDIFGAGDDDQGDDDSVD
                     FPETDDANFADAEEDMDEN"
     gene            complement(7058..>8544)
                     /gene="argS"
                     /locus_tag="PLM68_05765"
     CDS             complement(7058..>8544)
                     /gene="argS"
                     /locus_tag="PLM68_05765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT54906.1"
                     /translation="DIADTPAYGTKPHSGKTVMVEFSSPNTNKPLHLGHIRNNLLGWS
                     VSRIMEANGHRVIKVNLVNDRGIHICKSMLAWQKSGLNTTPESSGQKGDHLVGDFYIL
                     FEKQYKEQVQEMVNSGVDREQALKEAPLMQEAQSMLVKWEQGDGEVVGLWKKMNGWVY
                     DGFEQTYSRMGISFDKTYYESDTYLLGKELVVKGLEEGVFYRNPDNSVWCDLTQEGLD
                     RKLLLRADGTSVYMTQDLGTAVSRYKEYGFDSHIYVVGNEQNYHFQVLKLILKKLGFS
                     WADDIIHLSYGMVELPEGKMKSREGTVVDADDLIDNMVETAREMSLESGKLEDMTPQD
                     REQLFNMLGMGALKYFILKVDPRKTMLFDPRESIDFNGNTGPFIQYTFARIRSILRKA
                     GEHNYRSRLFNRPMIEKELRLIKLMTTYPLVVEESGLNYAPSLIANFGFDLAREFNQY
                     YHEISVLHEPDEQLRSQRLFLLEQVARLLESSMELLGIILPERM"
BASE COUNT         2225 a         2049 c         1921 g         2349 t
ORIGIN      
        1 ggaagggttc cggtggagtc ttgaaaggga ttatacctat atgatagaaa tggatgccga
       61 tttttcacac aatcccgagg acctgcccag gctagttgat gcatgccgtc aaagtgcaga
      121 cctggctata ggttcacgtt attgcaaggg tatcagtgta atcaactggc cactggggcg
      181 ggtactgatg tcctggtttg cctcggcttt tgtaagaaca atccttaata tgcccatata
      241 cgactgcaca gccggattta tttgttacca ccgcagggta ttggagggta ttgatttcag
      301 atgggtcaca atgaaaggat atggattcca gatatctatg aaatattatg cctataagct
      361 gggttttaaa ataatagaga tacctatcat atttgtggac agaacagaag gcacttctaa
      421 aatgagcagt ggcatattcg gggaagcttt ctggggggtc ctcaaactcc gctttaaaag
      481 gattcgtaaa aaataaagca tggccaggaa cattctcatc cggaacgcaa gcatagtaaa
      541 tgagggctcg gtttttcgtg gatgcgtggt tcttaaagac cagcatattg cggggatctt
      601 tcgtggtcat aaggcagaag aggaagctgt cgccttttgt ggaacccgtg atattgatat
      661 tgacgccagc ggtaaaatca tgataccggg aatcatagat gatcaggtcc atttcaggga
      721 accaggtcag cccggcaaag gcaccatgat gcaggaaagc cgttccgctt tgctgggtgg
      781 cgtaacgtcg gtcatggaca tgcccaacaa tgtgcctcct gttatcactg cagaattgtt
      841 ggaacagaaa tatatcctgg ccagagaaaa aatgtatgtc aattacgcgt tttacatggg
      901 agcgaccaac accaacaccc gggaagtgct gaaacctgta agacaatcat gcggattgaa
      961 gatatttatg ggttcctcaa ccgggaatat gctcacagac aatcactcgg tactgaatga
     1021 tttattctgc aactacaagg gagtgatagc tacccattgc gaagatgagt ccatgatcag
     1081 ggaaaatctg gccagcatga aagcccgtta tggtgactgt attcccttga accttcacag
     1141 cagcatacgc agcagggaag catgtattgc atctacccga aaggcattgg acctggctct
     1201 taaataccac tccagacttc atattcttca tatttcaaca aaagaagagg tggaattgat
     1261 tcgtgaagca cgctgtgtcc actccgggat cagttgtgaa gcatccttgc ctcatatgtg
     1321 gttcacggaa gaagattatg gaaggtacgg aacacaagtt aaatgtaatc ccgccataaa
     1381 aacggcagaa gacagggatg cgattctgaa agctcttgag aaaagggata tccaggccat
     1441 tggatcagat cacgctcccc acacatggga agaaaagcag cagaattaca cgaatgcccc
     1501 atccggaata ccactcatcc agcatacatg ccagatgatg atggagctag ttcaaagccg
     1561 gcagctatcc cttacttctt ttgtagacgc ctgttgtcat cagccttcaa tcctttttgg
     1621 aatcaggaac cgaggtttta tcagggaagg ttattatgca gacctggtcc tgatagatcc
     1681 ggaaaaagaa cagcaaataa ccaaatcaca gcttgaatat acctgtcggt ggagcccgct
     1741 ggaaggatat attttcagca catctgtatc ccacgtcttt ataaatggga tgctggcagc
     1801 agaaaacgga cagatcatcc agaaaccccc tgtcctgccc atcctgtaac atgaccacac
     1861 aaagaatcag agcatatctg tatatatgca cgacaacatt actgtgggga gtatcttttt
     1921 tctggaccaa caaacttctg ataaaccacg ttccagtatt tttcctgata ttgaccagga
     1981 tgtcactggc tgcgctcata cttcttattg ttggaagaat aacgggaaaa atccagcgaa
     2041 taaaaaacaa gaaagatgcc ttatggtttc ttgctctggc tctgatggaa ccattttttt
     2101 atttcattgg agagacttat ggggtaaaac tgacagcaag tccaacaata agctcaatga
     2161 tcatcgccac cataccggtt ttcggtcttg ttgcaggttt gagcttctat aaagaaaagg
     2221 tgacggcggg caacatcgca ggaatcctta tgaccgttcc cggtatgttg atcgtggtgt
     2281 caggaagagg aggtcttgaa acgggtaatg tgaccgacct gcccggaacc aatatgccat
     2341 tgggggccat gtttcttttt atggctgtct tttctgctgt gggacatacg gtgatcctga
     2401 aaaggatgac ccgttcttac aacgctttta cagtcacttt ctatcaacat gccctggggg
     2461 ctctttattt tatcctgccc gtgctgctta ttgatgccca acgcattaac gtgaagcatt
     2521 tcattacaaa agcagatttc tggtatccta tcctcatgtt gtcggttttc tgttccagta
     2581 ttgcttttat gatgttcacg gcagccataa aggaattggg ggtaacacgg accaacacat
     2641 tttctgccgt aatccccata gtaacagctt cggtaggttt tgccctgggt attgaaacaa
     2701 tcaactggat ccaggcttta ggtattttgg tggtagtctt tggcctggtg ctgtctcagc
     2761 tccgttcaag gcgtagctga attctgctcc tttcaagatc tatctttagt actttcacca
     2821 ttacatgctg gtgtaatttg acaacctgga gcggatcgtg aacgtatttt tcccccattt
     2881 gcgaaacatg gaccagccca tcctgtttga tgcctatatt gacaaatgca ccaaaatttg
     2941 tcacgttgct cacaatcccc ggaaggacca ttccttcccg gaggtcttct attgatgata
     3001 ttgattcatc aaactcccat acctttataa cagccctggg atcccttccc tgtttgtcca
     3061 gttctgtcat gatgtcacgc aatgttggta ttcctgtgac aggtgttata tatacagaca
     3121 ggtcaatttt cctgcgcagt tcttcttttt ctatcagttg ttccacagtt actcccaggt
     3181 cagctgccat tcgccttacc agcggataac tttccggatg aacccctgta ttgtccagta
     3241 tgtctgtggc tccagggatt cttagaaaac cggcacattg ttcaaacgct ttttctccaa
     3301 gtcttgatac ttttttcagc gtctgccggc ttgaaaaggg accgttttcc tgcctgtatt
     3361 caacaatatt gcgggcaaga cccgggccga gacccgatac ataggtcagt aacctcaggc
     3421 tggcagtatt gaggttcacc ccaacatggt tcacacaaaa ctcaactgtc tgatccaggc
     3481 tctctttcaa aagagtctgg ttgacatcgt gctggtattg ccctattccc agtgatttcg
     3541 gatctatctt gaccaattct gccaatggat ccataagcct tctgccaatg gaaaccgctc
     3601 cgcggacggt cacatcatag tccgggaact cttcgcgtcc tgcctgagaa gcagaataca
     3661 cactggctcc atcttcgctg acagaaaaaa tctggatctt atgaggaaat gctatttttt
     3721 taaggaattg ttcagtttca cgacccgccg ttccgtttcc cacagctatt acctctatct
     3781 tgtacgcctc tgtcatctgc gacaccttgc gcatggctgt agcacgctcg ttctgaggcg
     3841 gatgtgggta tattgtatcg ttatggagca ggtctccctg ggccgagagg catacgactt
     3901 tacaccctgt tcgaaatccc ggatcaatag ccatcaccct tttttgtccc agtggcggtg
     3961 ccagtagcaa ttgcctcagg ttttccgaaa aaacctttat ggattccctg tcggctttgt
     4021 ctttgtagta attcagcacc tcattctcaa tggagggatg aataagccgg cgatacgcat
     4081 cggacaaagc ctgttccatc tgccatacac tagaagatgg tccgtgcatg gtggatgatc
     4141 ctttcctgtt gcccatgaac attttttcca tttctgcaag gggcatggtg tccggatgtg
     4201 gcttcaagcc gagggaaacg atgccttcgt tgttcgctct gagcagtgca agcagcctgt
     4261 ggggagccat ggaactgatg ttttcgctgt aatcaaaata attcctgtac tttatcccct
     4321 cctcttcttt tcctttttga accatggagg caagaacacc actcctttga tagatatagc
     4381 gtaccttttg ccgtaacgtg gcgttttcac tcacagtctc ggcaataata tccctggcac
     4441 cagacaaggc atcttcaaca ctttcaactt cttcccccaa gtacccctgt gccatttcca
     4501 ggcaattctg ctcagggaga cccagcaaag cctgagccag gggttccaga cctttacttc
     4561 gggcaattga tgccctggtt ttacgttttg gcctgtacgg tagatagagg tcctccagct
     4621 tgttcatctg ccagcaattc tctatgaccg ctcgtagttc aggagtcagt ttttcctgat
     4681 cttcaatgga tctgagtatg gctgactttc ttttatccag ttcttcgtaa ctgttccaga
     4741 gtgatttgac tgccgccagc tggacctcat ccaatgatcc cgttgcttct ttacggtagc
     4801 ggcttacaaa agggatggta gcgccgtcct gaaacagtgt cactgcatgt tccacttgcc
     4861 agtctttgat gtgaagctca gaagctatca gttctatgtg gtttttattc atgggaaacc
     4921 tgggataatt gctctcggta agctgacaat atacgcgatt ttgaaacaaa tcccatatac
     4981 cggaatttct catccagtac aggcaggttc catgcatcag tcctttcgaa tttttccata
     5041 acgctttcca tccgttcatc tatgaacacg tattctaccg gccggtgcat cagttcgtag
     5101 acaaatacgt tcccgtacaa ttccctgcgg aacataatgg gacggatatc gtccagtgaa
     5161 acaactcctt gcaggcagcc ttccaggtca gtgaccggga aaaggttcct gctgctttgt
     5221 tcaacaacgt ggaccaggtg ttccagcgaa tcgtgcggat tcacagtcat aaaatcattt
     5281 tcaatgagat cagaggtttt cagcagcgtg agcacggcct ggtcactgtc gtgtgtcagt
     5341 aattttcctt ccctggcaat gcgtttggta tagatggaat aaggttcaaa tccacgggct
     5401 gtgacaaacg aacaaactgc ggtaatgatc agcggcacaa gcagttgata tccaccggtt
     5461 atctcggcga tcaaaaaaat tgccgtcatg ggagctttca ttaccccggc catcagccct
     5521 gccataccca caagggcgaa atttgattcc ggcaagcctg acacaccagc caggttcagg
     5581 gcccgggaaa taacaaatcc caggattcct cccatgaaca gggttggtcc gaaggtgccc
     5641 cctacacctc ctcccgcatt ggtaaaggac attgcaaaca ctttgagcag caaaaccgct
     5701 gtgaagaata tgggaatgaa ccatggatta cggctcaatg gaccgtaaat gagactgtcc
     5761 acagctcctt ccacattgtt gttcaacaag gcggaaagag acccgtaacc ctctccatac
     5821 agcggaggaa aaagcaaaat cagcacaccc aggatcaagg ccgaaaaggc ccatcgggta
     5881 tagatgttgt gaatcctttt gatgcgactt tccagcatga gcgtggtgcg ggtgaaaaaa
     5941 atactggtga agccacatac gattccgagt ataagataat aggggatgtt ggttatttgg
     6001 aagggagcgg ttgaacttgc aaaactcacc ccatcaccca tgatcaggga agatactacg
     6061 gtagccgtta tggaggctgt gagcagggga atcaaagagc caatcgtaag gttgaaaagc
     6121 agtatctcaa aacagaaaag cacacctgcc aggggggcct tgaatatgcc ggctatggca
     6181 gcagctgtcc cgcaacagac aagaagggtg acgctccggt aattcaaacc aaggcttcga
     6241 cccacattgg aaccaatggc tgcaccggta taaacgatgg gtgcctctgc ccctactgat
     6301 cctccaaagc caatggtcag agcacttgat atgatggatg tgtagcaatt atggggtttt
     6361 atcctggatt tgctgaccga tatggattcc agaacgcgtg taacaccatg tgatatattg
     6421 tctttgacaa agtatctgac aaaaagtaaa gaaagcagca tgcctgttcc gggaagaagg
     6481 aaataggcaa gactttctgc ctgaacgctc acgtgagttg tcaatagatc tttgataaat
     6541 gctatggagg atttgagaat tacagcggcc agaccgctga gaagcccaat gacaagcgac
     6601 aacaataaaa gcagcgtagg ctcgggtaaa ctcttaattt tcgtccatat cttcttcggc
     6661 atcggcaaag tttgcgtcat ctgtttcggg gaaatcgaca ctgtcatcat ctccctggtc
     6721 atcgtctccg gcgccgaaga tgtctttttc gatttcgtcg taatcatcaa tcttgacttc
     6781 aaccttaaca aggtatatgg catccggaat ttcaagcgta acagcgtaaa aaaacgagcc
     6841 gtccgcttta tcaaccttca tgatatcctc catgtaatcg atataacccc tgggatactt
     6901 ttctttccag atatccatca gctgggggga catattggca taaccgatta ccagtctttt
     6961 cttattgata gcaggcttta cttccgttgt ctttttcata ggacaaataa aaaaattgaa
     7021 tttgctgcaa taatacaata atattttgaa atccaaatta catcctttcc ggcaatatta
     7081 ttccaagcaa ttccatggaa gattcgagca acctggctac ttgttccagg aggaagaggc
     7141 gctgcgaacg aagttgctca tctggctcat gaagcacact aatttcgtga tagtactggt
     7201 tgaattccct ggcaaggtca aaaccgaaat tggctatcag tgaaggcgca tagttcaagc
     7261 ccgattcttc aaccaccagg ggataagtag tcatgagttt aataagcctc agttcttttt
     7321 ctatcatggg cctgttgaac aggcgggagc ggtagttatg ttcacccgct tttctgagaa
     7381 tggaacgtat gcgtgcgaaa gtgtactgga tgaaaggccc ggtgttcccg ttaaagtcaa
     7441 tggattccct gggatcgaac agcattgttt tcctgggatc cactttcaga ataaaatatt
     7501 tcaacgctcc catacccagc atgttgaaca gctgttccct gtcctgtggt gtcatgtctt
     7561 caagttttcc cgattcaaga gacatttcac gtgctgtttc caccatatta tctatcagat
     7621 catctgcatc gaccacagtt ccctccctgg atttcatctt tccttcagga agttccacca
     7681 ttccatagga aaggtgtatg atgtcatctg cccaggaaaa tcccagcttt ttgagaatca
     7741 gcttgagaac ctgaaaatgg taattctgtt cattgccaac aacgtatata tgcgaatcga
     7801 aaccatactc cttgtaccgg cttacagcag ttcccaggtc ctgcgtcatg tacacagatg
     7861 ttccgtctgc tcggagcagc agtttccggt ctagtccctc ctgtgtaaga tcacaccata
     7921 ccgaattgtc cggattcctg taaaaaacac cttcttccag ccctttgact accaactcct
     7981 tacccaatag gtaagtatct gattcataat aggttttatc aaaggaaatg cccatcctgc
     8041 tgtaggtttg ttcaaatccg tcataaaccc aaccgttcat ttttttccaa agtccgacaa
     8101 cctcaccatc tccctgttcc catttcacaa gcatggactg ggcttcctgc atcagtggcg
     8161 cttccttgag ggcctgctca cggtccaccc cgctgttgac catttcctgt acctgttctt
     8221 tgtattgttt ctcaaaaagg atataaaagt ctcctaccag gtggtctccc ttttgccctg
     8281 acgactccgg tgtggtattc aatcctgatt tctgccatgc caacatggat ttacagatat
     8341 gtattcccct gtcgttgacc agattaactt tgattacccg gtgaccgttg gcttccatta
     8401 tcctggacac ggaccagccc agcaaattat tgcgaatatg tcccaaatga agcggcttgt
     8461 ttgtgtttgg agaagaaaat tccaccatta ccgtcttacc ggaatgaggc ttggtgccat
     8521 aggcgggtgt gtcagcaatg tcct
//
LOCUS       DAOSOF010000083         8417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8394_length_8417_cov_5.555848, whole genome shotgun sequence.
ACCESSION   DAOSOF010000083 DAOSOF010000000
VERSION     DAOSOF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8394_length_8417_cov_5.555848"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLM68_05770"
     CDS             complement(<1..646)
                     /locus_tag="PLM68_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOT54907.1"
                     /translation="MDGILIENIRQLVQVRETPGDNPSWCGGHMMNRLPCIENAWLFI
                     RDGVIDGFGPMERNPYREDIPLGTTIIDATGKCVFPSFCDCHTHIVYAGSREQEFTDK
                     IRGLSYKEIAARGGGILNSSRLLRETSEEELYRQSLVRARELIRFGTGAVEIKSGYGL
                     TVEDELKMLRVIRRIGQETPLTVRATFLGAHAVPEQYKGNQSGYVDLIIEEMIPR"
     gene            complement(627..2120)
                     /gene="hutH"
                     /locus_tag="PLM68_05775"
     CDS             complement(627..2120)
                     /gene="hutH"
                     /locus_tag="PLM68_05775"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOT54908.1"
                     /translation="MNLHTISPHPVDTGIPEKVIEGNIRLELSEESRRLIDQCRQYLD
                     KKVRNSDGLIYGVNTGFGGLHNVAVGNKDLERLQNNLVMSHACGTGPQVPEVIVRLML
                     FLKIRSLSYGYSGVQLKTVERLVDFFNMGVIPVVYEQGSLGASGDLAPLAHLALPLIG
                     LGEVDFRGKRISGSELNRQMGWEPIALQSKEGLALLNGTQFMCAFGLWCLVQAQRLAD
                     QADMICALSLDAYDGRPEPFTKEVHQIRPHAGQAASAARIEEYLEGSELARRHKEHVQ
                     DPYSFRCAPQVHGATRDCIEYVSRVMTTEMNSATDNPTIFPSLDKVVSAGNFHGQPLA
                     LALDFLAIAVAELGSISERRTNQLVLGKRGLPPFLVARPGLNSGFMIPQYTAASIVSR
                     NKQLCTPCCVDSIDSSQAQEDHVSMGANSALKCFSVVNNVRQVLAIELFNAAQALEFR
                     RPSQTSARLEQLVAEFRKHVPFIEEDTTMYPHLHKADNFLKDGWHFN"
     gene            2276..5434
                     /locus_tag="PLM68_05780"
     CDS             2276..5434
                     /locus_tag="PLM68_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54909.1"
                     /translation="MKKSLFFALAVLITTAAFAQSRVTGKVTSSEDGSPLPFVTILIQ
                     GERGLGTNTDIDGNFILERCPSDAILVLSYVGFTTIQVPVEGRNVINVTMNPDALALE
                     EVMVVAYGTARKGTYTGAASVVKSEAIKDVPTLSFENALNGKVAGMQITTNSGQAGSV
                     SSIRVRGIGSMNASNEPLYVVDGVPVSSGSTGQMSGYIYSTNNVMSTLNPQDIESITV
                     LKDAAASALYGSRAANGVILVTTKTGRLGRPVVNFKASVGITPSFATVNFDIASPEQQ
                     VELYYENFWNAGKYDGKTDEASSKSALSQLNKRFNKHGYYFTADDNTVNSLKILGMTD
                     GKENREGKYYNWDDVLFRTAIYQTYDLSVSGASDRTSYYSSLSYTKEQGRSTVNDFSR
                     IAGRVNVNQKVGKIIEFSTNVNISKNVQHGFNDTRSTGGNYFMQSRNLLWPLYWPTDY
                     KTGDPWTARYGSYAYNQVYYNNEWENESNVLRLSANETLTVKFMPELVLKSVLSFDNT
                     ETKDHIYYSANHFNASSVKGQVHEMSTNVQKIVSSTTLNYDKEFADKHIVNVLVGWEA
                     EDNQTNYQRSSGTNLPTSALHTVATAGVLDASAYYWGSTMLSFLSRAEYSYDGRYYLS
                     GSFRRDGSSRLGSNTRWGNFWSVAGSWRINNEAFMKDITMISNLRLRGSYGVNGTLPS
                     SNYGWRALTSYGNKYMEDPGGGVANIADANLSWETSYTWNAALEFGLFNNRLNGTIEY
                     FNRDSKDLLQSVPISTVTGFGSTLKNIGEINNHGLEIELSGDIIRTSDLRWSAGLTGS
                     WIRSTVTKLYGGKDIYWYDPTGDDARAKFVYREGESTLALYGLEWAGVEDETGKNVWF
                     LNNDSEADLTVDGRPATYNYSNASEVILGDAHPDFFGGFATDLSWKGLSIALNFVYKI
                     GGYTYNAVGRDVNDDGYYWERIMSQYCYDNRWTPENKTAKYPQRVAIDMEDVNQKSSR
                     HMNPADYLRLKNVTVSYTLPRTWTSKISIQNARIYFNGSNLWTLAAHKEYDPEVNEYG
                     SRGWEIPLEGPSPSALNLVFND"
     gene            5447..6925
                     /locus_tag="PLM68_05785"
     CDS             5447..6925
                     /locus_tag="PLM68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54910.1"
                     /translation="MKKINILLAAICVVLTMSCEGFLDVKPSNSAAAETSITTAADAK
                     VIMNGIMSKMTSSSYYGRNFIMYGDAKGGDFALRSQGRGLDALYTFNHSPSSNSYSGY
                     WSQIYHCLAQVNNLLVNIQKIEDAGNSTSALLVYKGQALTLRALMYFDLVRLYGKPYN
                     MDKTALGVPLALEPLDASSQPSRATVEQVYTQIVKDLTDGAPLLNKTKTNGYINYYAN
                     RAILARAYLYMENWGGALAAAEEIINSKVYTLYTNANWVSSWTAQYNTESIFELAMLQ
                     GESDLEEGSLSFYLRRLGHGSTSAMGWFMASDYWIQRMSQDPDDIRWGIMDYDESYKD
                     TGVYRFGSCYKYSGSTTFVGDGKGSSTAVNIKVIRLSEIYLIAAEAALNSNSKDKAVN
                     YLNAIRKRSPNLAPATATNITSDMILDERSKELFAEGHRFFDMMRLNKTITFNDDFIY
                     PMVVITHREKTIDRTFYKTILPISIDEMNANEGVRSQQNPGY"
     gene            complement(6922..>8417)
                     /locus_tag="PLM68_05790"
     CDS             complement(6922..>8417)
                     /locus_tag="PLM68_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT54911.1"
                     /translation="DSMVNHYTAARRLRSNDAYTPGGRAGMRPDYAVTVYTRILKQLY
                     PNVPVVLGGIEASLRRLTHYDYWTDTLKPSVLIDSGADYLVYGMGERPIVELAKKLRE
                     HAALEEIRIIPQIAYLAARPDEISCDPEKILYLPSHKECRNSKKQFAEHFRIFEQKAN
                     SSNPPVMAEPADAKEGITVVVNPPYPLMTTEELDAVYELPFTYQAHPRYKGKSIPALE
                     MIRFSVCIHRGCFGGCSFCTIAAHQGKRISSRSLESVTTEIARLATLAEFRGHLSDLG
                     GPTANMYRMQGRNPALCDACNRTSCLFPVVCKNLDTSHKSLLELYRTVRNIPGVKKAT
                     IGSGIRYDLFVNEKGFLNDEGKQYFRELVQYHVSGRLKVAPEHTQDNVLSMMHKPSFA
                     LYRVLKREFLSETKRTGSRLQLIPYFISSHPGCTQRDMNLLAGIFKSEKIRVEQVQDF
                     TPTPMTKSSVMYYTGLVPETGEQVFAERDPQKKALQKEGFFKNNRKS"
BASE COUNT         2116 a         2165 c         2025 g         2111 t
ORIGIN      
        1 ccctggggat catttcttct atgatcaggt ctacatatcc ggactggttc cccttatact
       61 gttccggaac cgcatgcgcc cccagaaagg ttgcccttac cgtcaggggc gtctcctgtc
      121 ctatccttct gatgacacgc agcattttca attcgtcctc aacggtaagg ccatagccgc
      181 tttttatttc aaccgcccct gtgccaaagc ggatcagttc cctggcccgt accaggctct
      241 gccggtaaag ctcttcttca gatgtttcac gcaacaggcg ggaggaattg agaatgccgc
      301 ctccgcgtgc cgctatttcc ttataagaca atccgcgtat tttatctgtg aattcctgtt
      361 cccggctccc ggcataaaca atatgcgtat ggcaatcaca aaaggaagga aaaacacatt
      421 ttccggttgc atcaatgatc gtggttccga gaggtatgtc ttcacgatag ggattccttt
      481 ccatggggcc gaatccgtca atcaccccgt cccttatgaa cagccaggca ttttcaatac
      541 acggcagccg gttcatcata tgacccccgc accatgaggg attgtcaccc ggggtttctc
      601 tgacctggac cagctgccgg atattttcaa ttaaaatgcc atccatcttt taaaaaatta
      661 tctgccttat gcaggtgcgg atacatggtg gtatcttctt caataaaggg aacgtgttta
      721 cggaattcgg ccaccagttg ttccagccgg gcagaggttt gtgagggtct tctgaattcc
      781 agggcctgcg cggcattgaa taattctatc gccagcacct gtcgcacatt gtttaccaca
      841 gagaaacact tgagtgccga attggctccc atgctcacat ggtcttcctg ggcctgtgaa
      901 gaatcgatgg aatccacaca acagggtgta cacaactgtt tgttccggct aacaatggag
      961 gctgcggtat actgcgggat catgaatccg ctgttgagtc ccggccttgc caccaggaat
     1021 gggggtaatc cccttttccc cagaaccagt tggttggtcc ttcgttcaga tatgcttccc
     1081 agttccgcca cggcaatagc cagaaaatcc agagccagag ccaggggttg tccgtgaaag
     1141 tttccggccg agacaacctt gtccagcgaa ggaaatatgg tgggattgtc tgtggccgag
     1201 ttcatctctg tggtcatgac cctggaaaca tattcaatac agtcccgggt ggctccgtgc
     1261 acttgagggg cgcagcggaa cgaatacgga tcctggacgt gctccttgtg ccgtcgtgcc
     1321 agttcgcttc cttcaaggta ttcctcaatc cgtgcggccg atgctgcctg cccggcatgg
     1381 ggcctgatct gatgcacttc tttagtgaaa ggctctggcc ttccatcgta tgcgtccaga
     1441 gacagggcac agatcatgtc tgcctggtct gcaagtcttt gtgcctgcac aagacaccac
     1501 aatccgaagg cacacatgaa ctgggttccg ttaagcaggg caagaccttc cttggattgc
     1561 agcgctattg gctcccagcc catctgcctg ttcagctcgc tgccggagat cctcttaccc
     1621 ctgaagtcca cctctcccaa ccctataagg ggcaacgcca ggtgggccag gggagccagg
     1681 tcccccgaag cccccagaga tccctgttca taaacaacag ggattacacc catgttgaaa
     1741 aaatccacca gacgttctac tgtcttgagc tgtaccccgg aatacccgta tgaaagagac
     1801 ctgatcttga gaaagagcat gaggcgcacg atcacttcag gcacctgagg acctgttccg
     1861 catgcatggg acattaccag gttgttctgt aaccgctcca ggtctttgtt cccgacggcg
     1921 acattgtgca atcccccgaa tcctgtatta acaccataga tcaatccgtc tgaattgcgc
     1981 acctttttat ccaggtattg gcggcattga tcgattaacc ggcgtgattc ttcagataac
     2041 tccagacgta tattcccttc tatgactttt tcagggatcc cggtatcaac gggatgagga
     2101 ctgatagtat gaaggttcat gcgtgctttt attaatattt cacaacaaaa ttaccttatt
     2161 ttaccaaaaa ttaaaaagga atcagtctat tgccggaaaa attttgtttc ttacctttgt
     2221 ttactttaca ttgttacttt ttataaacct ttattactaa cctaaccaaa ttttcatgaa
     2281 gaaatcttta ttttttgcgc tggcagtgtt gattacaacc gctgcttttg cgcaatcgag
     2341 ggttaccggt aaggtaacat cttcagaaga cggttctccc cttccctttg taactatttt
     2401 gatccaggga gaaagagggc tgggaacaaa caccgacatt gacgggaatt ttattcttga
     2461 aagatgtcct tcggatgcaa ttcttgtctt atcctatgtc ggttttacaa ctatccaggt
     2521 ccctgtagaa gggaggaacg tcattaatgt caccatgaat ccggatgccc tggcattgga
     2581 agaggtcatg gtggtggctt acggaactgc cagaaaaggg acctataccg gagccgcttc
     2641 tgtggtgaaa tccgaagcca taaaagacgt ccccacactt tcctttgaaa atgctctgaa
     2701 cgggaaagtg gccggtatgc agatcacgac caattccggt caggccggtt ccgtttcgag
     2761 catacgtgtt cgcggtatcg ggtccatgaa cgcttccaat gaaccgcttt acgttgttga
     2821 cggggttccc gtgagttcgg ggagcaccgg gcaaatgagt ggctacatct attccaccaa
     2881 taacgtgatg agcacactca atccccagga cattgaatca atcaccgttc taaaagacgc
     2941 cgccgcttct gcgctatacg gctcccgcgc tgccaacggt gttatcctgg tgacaaccaa
     3001 aaccggacgc ctgggaagac cagtggtcaa tttcaaggca tctgtaggga tcactccctc
     3061 ctttgccact gttaactttg acattgccag ccccgagcaa caggttgaac tgtattatga
     3121 aaacttctgg aatgccggca aatacgacgg caaaaccgat gaggcctcca gtaaatctgc
     3181 cctgtctcag ttgaacaagc gttttaacaa acacggatac tatttcacag ccgatgataa
     3241 caccgtgaac tccctgaaaa tactggggat gactgacgga aaggaaaaca gggaaggcaa
     3301 atactataac tgggacgatg ttctgttccg cacggctatt taccagacat acgacctgtc
     3361 tgtgagcggc gccagcgaca gaaccagcta ttactcatcc ctttcctata caaaggaaca
     3421 gggacgcagt acggtcaacg acttcagccg tatagccggc cgcgtgaacg tgaaccagaa
     3481 ggtgggcaag attattgaat tcagcaccaa cgtgaacatt tccaagaacg tgcagcacgg
     3541 cttcaacgat acccgcagca ctggaggcaa ctacttcatg caatcacgca accttttgtg
     3601 gcccctttac tggcccacgg actacaagac aggtgacccc tggacagccc gttacggaag
     3661 ttatgcctac aaccaggtat attacaacaa tgaatgggaa aacgaatcca atgtgctgcg
     3721 tttgtcagcc aatgaaacac tgaccgtgaa gttcatgccg gaactggtat tgaaatccgt
     3781 cctttcattt gacaacacag aaaccaagga tcacatctat tacagcgcaa accactttaa
     3841 cgcttccagc gtgaagggtc aggtacacga aatgtccacc aatgtacaga agatcgtatc
     3901 gtctaccaca ttgaattacg acaaggaatt tgcagacaag cacattgtga atgtactagt
     3961 gggatgggaa gcagaagaca accagactaa ttaccagcgt tcttccggga ccaacctgcc
     4021 taccagcgcc ctgcatacgg tagccaccgc cggggttctg gatgccagtg cctactattg
     4081 gggcagtacc atgttatcct tcctctcacg cgccgaatac agctatgacg gacgttatta
     4141 cctgtccggg tcattccgcc gtgacgggtc ctctcgcctg ggctccaaca cccgctgggg
     4201 gaacttctgg tcagttgccg gttcctggag gatcaacaat gaggctttca tgaaagacat
     4261 aaccatgata agcaatttgc gtctgagagg ttcctacgga gtgaacggaa cgcttccctc
     4321 aagcaactac ggatggcgtg ctctgacctc ctatggcaac aagtacatgg aagatccagg
     4381 tggtggtgtg gccaacatag ccgatgccaa cctgtcctgg gaaaccagct acacctggaa
     4441 cgctgccctg gagttcgggt tgtttaacaa ccgtctgaac ggaactattg agtattttaa
     4501 cagggactcc aaagacctgc tgcaaagcgt tcccatttct actgttaccg ggttcggatc
     4561 aaccctgaag aatattggtg agatcaacaa ccatggtctg gaaattgaat tgagcggcga
     4621 tattatccgc acttcagatt tgcgctggag tgcgggtctg accgggtcct ggatccgttc
     4681 tactgtaacc aaactttatg gcggcaaaga catttactgg tatgatccca ccggggatga
     4741 cgcccgcgcc aagttcgttt accgtgaagg cgagtccacc ctggccctgt acggccttga
     4801 atgggccgga gttgaagatg agaccgggaa gaatgtctgg ttcctgaaca acgattcgga
     4861 ggccgatctt acggttgacg gccgtccggc aacctacaac tattccaatg cctctgaagt
     4921 gatcctgggt gacgcccatc ctgatttctt tggaggattt gccaccgatc tgagctggaa
     4981 aggactcagc attgccttga attttgtata caagatcgga ggatacacct acaatgccgt
     5041 tggtcgtgac gttaatgacg atggttatta ctgggaacgt atcatgtcac agtattgcta
     5101 tgacaaccgc tggactccgg aaaacaagac cgcaaaatac ccgcagcgtg ttgccattga
     5161 catggaagat gtgaaccaga agagcagccg ccatatgaac ccggccgact accttagatt
     5221 gaagaatgta acggtttcct acaccctccc gcgcacctgg accagcaaga tcagtatcca
     5281 gaatgccagg atatacttca acggttcgaa cctgtggaca ctcgcagctc ataaggaata
     5341 cgaccccgaa gtgaacgaat atggatccag gggatgggaa attcccttgg aaggaccttc
     5401 accttcggcc ttgaatttag tttttaacga ttaactgaac aacactatga aaaagataaa
     5461 catattacta gcagctattt gcgttgttct gaccatgtct tgtgaaggat tcctggatgt
     5521 aaaacccagt aattccgccg cagccgaaac gtccattact acagcggccg acgccaaagt
     5581 gatcatgaac ggaattatga gtaagatgac atcctccagc tactacggac gtaactttat
     5641 catgtacggc gatgccaagg gtggtgattt tgcgcttcgt tcccagggca ggggtctgga
     5701 cgctttgtat accttcaacc actccccctc ttccaattcc tattccgggt actggagtca
     5761 gatctaccat tgtctggccc aggtaaacaa cctgcttgtc aatatccaga agatagagga
     5821 cgcaggcaat tccaccagtg ccttgctggt atataaagga caggcactta cacttcgcgc
     5881 cctgatgtat tttgacctgg tacgcctgta tgggaaaccc tacaacatgg ataaaacagc
     5941 cctgggtgtt ccccttgcac tggaacccct ggatgcctca tcgcaaccct caagggccac
     6001 cgtggaacaa gtctacacac aaatcgtgaa agaccttact gacggagccc ccctgttgaa
     6061 caaaacaaag accaacgggt acattaacta ctatgccaac agggctatcc tggcccgtgc
     6121 ttacctgtac atggaaaact ggggtggtgc cctggccgct gcagaagaga tcatcaattc
     6181 caaggtatat accctgtaca ccaatgccaa ttgggtcagc tcatggactg cccagtacaa
     6241 taccgaatcg atatttgaac tggccatgct gcaaggtgaa tcagacctgg aagaaggctc
     6301 gcttagcttt tacctcagac gccttggtca cggttctacc tcggccatgg gctggttcat
     6361 ggccagcgat tactggattc aacgtatgag ccaggatccg gatgatatac gctggggcat
     6421 aatggactac gatgaatctt ataaagatac gggtgtatac cgttttggtt cctgctacaa
     6481 atacagcgga agcactactt ttgtcggtga tggcaaaggc tcttccacgg ctgtgaatat
     6541 caaggtgatc cgtttgtccg agatctacct gatagctgca gaagctgccc tgaattcgaa
     6601 cagtaaggat aaagcggtca attatctgaa cgccattcgt aaacgctctc ccaacctggc
     6661 tccggcaacc gccacgaaca ttacttctga catgatactg gacgaaagga gcaaggaact
     6721 gttcgcagaa ggtcatcgct tttttgacat gatgcgtctg aacaagacca tcacctttaa
     6781 cgatgatttt atttacccca tggtagtgat cacgcaccgt gaaaaaacca ttgaccggac
     6841 attctacaaa accattctgc ccatttcaat agatgagatg aatgccaacg aaggcgttcg
     6901 ttcacaacag aatccgggct attaggattt acggttattt ttgaaaaagc cttccttctg
     6961 gagggctttt ttttgtgggt cacgttctgc aaatacctgt tctcctgttt cgggaacaag
     7021 tcctgtatag tacatgacag aagatttggt catgggagtg ggggtgaaat cctgaacctg
     7081 ctccacccgg atcttttcac ttttaaagat ccccgctaac aggttcatgt ccctttgcgt
     7141 gcatcccggg tgggaagaaa tgaaataagg gatcaactgt aaacgggatc ctgtcctttt
     7201 tgtttcactc aggaattcac gttttaaaac cctgtacaat gcaaaagaag gcttgtgcat
     7261 catgctgagc acattgtcct gcgtatgttc aggagccact ttcaaccttc ccgatacatg
     7321 atactgtacc aattcccgga aatattgttt tccctcgtca ttaaggaatc ctttttcatt
     7381 tacaaacaga tcgtaacgga tcccgcttcc tatggtggct tttttcacgc cggggatatt
     7441 tctgacggtc cggtacagtt ccagcaatga tttatgggat gtgtccaggt ttttacacac
     7501 aaccggaaaa aggcaggagg tcctgttgca ggcatcgcat agtgccgggt ttctgccctg
     7561 catgcggtac atattggctg tagggccgcc aaggtctgaa agatggcccc tgaattctgc
     7621 cagggtggca agtctggcta tttccgtagt taccgattca agggagcggg atgaaatccg
     7681 ctttccctgg tgggccgcaa tggtacagaa actgcatccg ccaaaacatc ccctgtgaat
     7741 acatacagaa aaccggatca tttccagggc agggatgctc tttcctttgt aacgggggtg
     7801 ggcctggtag gtaaaaggaa gctcgtatac cgcatccagt tcttccgtgg tcatgagcgg
     7861 ataaggaggg tttaccacaa ccgtgatgcc ttcctttgca tctgcaggct ctgccatcac
     7921 aggcggattg gaggagttgg ccttttgttc gaatatccgg aaatgttccg caaattgttt
     7981 tttgctattg cggcattcct tgtgggaagg caggtacagt attttttcag ggtcgcagga
     8041 tatctcgtcc ggccgggcag caaggtatgc tatctgtgga attatccgga tttcctcaag
     8101 ggcagcatgt tcccttaatt ttttagccaa ttccacgata ggcctctctc ccatgccata
     8161 gaccaggtaa tcggcccctg aatctatcaa aacggaaggt ttcagggtat ctgtccagta
     8221 atcgtaatgc gtcagccggc gcagggatgc ttctatgccc ccaagaacta ccggaacatt
     8281 cgggtagagt tgtttcagga tgcgggtata cacagttacg gcataatctg gtctcatccc
     8341 ggcccttccg ccgggcgtat atgcatcgtt gctgcgcagg cgcctggccg ccgtataatg
     8401 gtttaccatg gaatcca
//
LOCUS       DAOSOF010000084         8254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8630_length_8254_cov_6.007562, whole genome shotgun sequence.
ACCESSION   DAOSOF010000084 DAOSOF010000000
VERSION     DAOSOF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8630_length_8254_cov_6.007562"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1750)
                     /locus_tag="PLM68_05795"
     CDS             complement(11..1750)
                     /locus_tag="PLM68_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT54912.1"
                     /translation="MGKGRPFSASTQFKMIIHTNGNHRYASTKPYTIGEDGIKRYTYK
                     HWGTLDEQNRFIPGMAYMYASIEEREQLIFPKDWDLSQIETISGTKRRGRIVYEAEDV
                     DRQFGPTWLLNQVAVKTGLLDDLRSVFSGNMEMVNDILTLAYFPFIDNLSYNQLSQWQ
                     REVKAPSGRDLNSVNITRLAQSITEQNRMDIFRCRAARLGKDDLCAVDSTSMPTYGFN
                     LVDIRWGRNKEHLPLRQTVELVVYSLTTHMPIYYKELPGNMPDVRTIELIMIELRHAG
                     FKNLVLITDRGYESVKNLELYISRGQKVITSVKVSQGDVLKKIKEIDMSHGYPKGMTL
                     AKKENLYYAQYGMDYTVHGKGDALIKADKYRLNLYFNPAKRAEAMCDIQHAVDEQAEA
                     AQAMMETGEPVSDRDEIKKRFNMLFIKFEANNRIASFEVNREKQDALLLTAGFFASKT
                     IGVDLDPLAARDNYGMRDEQEEAFALQKGALGQDRLRTWSETSKHGRMFITFIGLILV
                     SYVRATWKGNEVLKKKFGSTEAVLAEMRTIRYIEHSGKFKFITPFVGAQIDICKAFGC
                     TRPLLLGQKSQNG"
     gene            1922..2668
                     /locus_tag="PLM68_05800"
     CDS             1922..2668
                     /locus_tag="PLM68_05800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT54913.1"
                     /translation="MINYISDYKENHHLRESFNLLAKQTFGIDFETWYQYGFYDHSYV
                     CHSFVENGRVISNVSATKMQLIIDGKDINAIQIGTVMTCPECRMSGLAGRLMHTVIEE
                     YEPHNYMIILFPNETVMDFYPRFSFRLTNDTLYSIKTKCSGEAFDKFRKLDIKESAGR
                     RIIEQAVKTKIPLSERMDVRANHAICLWHCLNSFQDDIYIADSGLCVVIYKMSGDRID
                     LYDVITPFKILIGDILPALSGGEEKCVGLG"
     gene            complement(2782..3492)
                     /locus_tag="PLM68_05805"
     CDS             complement(2782..3492)
                     /locus_tag="PLM68_05805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT54914.1"
                     /translation="MENVNSSTKSYPIPRIGEPAPQFKAITTQGDINFPSDYKGKWVI
                     LFSHPADFTPVCTSEFMTFASMEEEFKALNTELVGLSVDGLYSHIAWLRTIKEKIQYK
                     GMKNIEVKFPLIEDITMEVANLYGMVQPGESNTKAVRAVFFIDPKGIIRTIIYYPLSL
                     GRNFDEIKRVLIGLQTVDEFKVALPADWRPGDDVIVPTAGSCGQAKDRLEIPEEGVTC
                     YDWFFCTKKLEKEKIEKA"
     gene            3614..4543
                     /locus_tag="PLM68_05810"
     CDS             3614..4543
                     /locus_tag="PLM68_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HOT54915.1"
                     /translation="MTLQQLQYIVALDRHRHFVKAAGECGISQPTLSAMISRLEEELD
                     VRIFDRSKHPVEPTPMGEEILHQARLVLKQAMQVQELVQAGKESLDTRFSLGVIPTVA
                     PYIVPQFIGLFKKDFPLTRVSILEMRTDEIIRDLKEAKIDMGLLATPLLDPDLLEIPL
                     YYEKFLAYIAPSDPNYPKKELSATELPLQHLWVLQEGHCLRNQVFNFCTDNTPADKQV
                     YEAGSIDNLVRIIDTNGGYTLIPQLHLPMLDEIQSTHVRPVNNPPPVREISIAVYKDF
                     YRERLLNAVANTIKQIIPADMLDTRLKKFAIRL"
     gene            complement(4550..5701)
                     /locus_tag="PLM68_05815"
     CDS             complement(4550..5701)
                     /locus_tag="PLM68_05815"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOT54916.1"
                     /translation="MERTKLLDSCFSFEREVTLPGKPVVTLPFEFTRELGREEVSQLQ
                     KSIQSAPFCPAADSTIGENQQFKYVIFAPAGADRFDRAIILLHGLNERSWNKYLTWAE
                     DLVMTCGVPVVLFPIAFHMNRTPAAWVTPRGLVPWLNRRKQEIKNLCNASFFNLALSS
                     RLSVSPLRFYSSGLESAFNLIQLINDMKQGRHPLFTESVRTDFFAYSIGALLAQVVLM
                     ADPYENLPDSRLFTFCGGSVFERMNGNSRDIMDQEAYQKIREYYLGEFHNFQQGQDGR
                     QAGVSDKLTNAFRLMIRSADPEDGRRMFFAGAKDRILMISLRQDTVIPTVGLHEATGQ
                     ELAGYMTRELDFPYEYTHQSPFPENGKIHETELITSFRSVFDRAAGFLA"
     gene            5839..6678
                     /locus_tag="PLM68_05820"
     CDS             5839..6678
                     /locus_tag="PLM68_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT54917.1"
                     /translation="MRRKIPPFIYKRSNLTLVVAFTALFALLFINIYKPFESPQWYPI
                     TQFEYFLYSSLIILTGVLVVVISRIIMYYYTKKRTLTYWEYLIWVIAEIFFMSLFYTL
                     YSYTLDETRDFTEVYRSSIIKTSLILLLPYIISLLFLSWREKSMRLKELEVKGQEEGV
                     PARTEIINFRDEKGVLQLSIKKDSLLYLESADNYVSVWYLSKSGVSNYLVRNTLKEME
                     LRFSQTRIVRCHRSYLVNLDQVKVARRTSNGIVLDLGTPKIPDIPVSRTHAEQIARWF
                     TNM"
     gene            complement(6684..7124)
                     /gene="mscL"
                     /locus_tag="PLM68_05825"
     CDS             complement(6684..7124)
                     /gene="mscL"
                     /locus_tag="PLM68_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT54918.1"
                     /translation="MSKFLKEFKAFAVRGNVVDMAVGIIIGAAFGKIVSSLVSDLLMP
                     LVGALVGGVNFTDWKWTIVGERASILDPNVMLPAVTLNYGNFIQVLIDFIIIAFCVFL
                     LIKGLNKLTSAKKKEEAPGAPAPEPEPTREEVLLTEIRDLLKKK"
     gene            7224..>8254
                     /locus_tag="PLM68_05830"
     CDS             7224..>8254
                     /locus_tag="PLM68_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HOT54919.1"
                     /translation="MKCRLIITALLFTTLTSLAARDPEGRLLRFPATGNGLIAFSYAG
                     DLYTVPLEGGNASRITSHNGYEVFPKFSPDGRTIAFTAQYDGNTEVYTIPAEGGEPKR
                     LTYTATLDRDNIGDRMGPNNIVMGWTTDGKNVVFRSRWYSFSGMRAHLFTVPAVGGEL
                     SQIPTSEGGFCSYSPDGQYLALNRMFREFRTWKYYKGGQADDIWVNKLGTTQLENITA
                     GEGDNAQDIFPMWIGTDIYFISDRDSIMNLFRYDTGTKKTEKMTRFDTYDIKFPSAWG
                     EYIVFENGGYIYSFNTRNRELNKVEITLHEENLHARTRLMDVSAQAANYSLSPNGERV
                     LVTSRGDVFS"
BASE COUNT         2146 a         1995 c         1910 g         2203 t
ORIGIN      
        1 cgggaatagc tcaaccattt tgactttttt gtccaagtaa taggggtctg gtacagccga
       61 aggctttgca gatatctatt tgggcaccaa caaatggggt gataaacttg aactttccac
      121 tgtgttcgat atagcgtatg gtgcgcattt cagctaatac agcctctgtt gaaccgaact
      181 tcttcttgag aacctcgttt cctttccatg tggcacggac ataagatact aaaatgagtc
      241 ctataaaggt gatgaacata cggccgtgct tgctggtctc agaccaggtt ctgagcctgt
      301 cctgacccag tgcccccttc tgaagagcaa aggcttcctc ctgctcgtca cgcataccgt
      361 agttgtccct ggctgcaagg ggatcgagat cgacaccaat ggtcttgctg gcaaagaagc
      421 cggctgtgag caacaaggca tcctgttttt cacggttgac ctcaaaggat gctatacggt
      481 tgtttgcctc aaacttgata aagagcatat tgaagcgctt cttgatctca tccctgtccg
      541 aaacaggttc ccctgtttcc atcatggcct gtgcggcttc agcctgttca tccacggcgt
      601 gctggatatc acacatggcc tcagcgcgtt tggcaggatt gaagtagagg ttgagcctgt
      661 acttgtcggc cttaatcagg gcatcgccct tgccatgcac ggtataatcc atcccgtact
      721 gggcatagta aagattctcc tttttagcca gagtcatacc tttaggataa ccatgactca
      781 tgtcaatttc ctttatcttt ttaaggacat cgccctgtga gaccttgacg ctggtaatca
      841 ccttctgccc tcttgagata tagagttcaa ggtttttgac cgactcgtaa ccgcggtcgg
      901 tgatcagtac caggttcttg aaaccggcgt gccgcaattc tatcatgatc agttcaatgg
      961 tacgcacatc cggcatgttg ccgggcaatt ccttgtaata gatgggcata tgcgttgtca
     1021 gtgaatagac cactaactcg acggtctgac gcagcggaag gtgctccttg ttccttcccc
     1081 aacgtatatc caccaggttg aacccgtacg ttggcatgga tgtggaatca acagcgcaca
     1141 gatcatcctt gcccagtctg gcagcccgac agcggaaaat atccatcctg ttctgttcgg
     1201 tgatgctttg cgccagcctg gtgatgttca cgctgttcaa atccctcccg gatggagcct
     1261 tgacctcacg ctgccattgt gatagttgat tgtaggagag attgtcgata aatggaaagt
     1321 aagccagggt gagtatgtca ttgaccatct ccatgttgcc tgaaaaaacg cttcgaaggt
     1381 catccaggag acctgtcttg actgccacct ggttgagcag ccatgttggg ccaaactgtc
     1441 tgtcaacatc ttcggcttcg tatacaatgc gaccacgacg tttagtcccg gaaatggtct
     1501 ctatctggct caaatcccaa tccttgggga aaatgagctg ttcgcgctct tctatggatg
     1561 catacatgta agccatgccg ggaatgaagc ggttttgttc atcaagcgta ccccagtgtt
     1621 tataggtgta acgcttaatc ccatcctcgc ctatggtgta gggtttggtg gatgcatatc
     1681 ggtgattccc gttcgtatga atgatcatct taaactgcgt ggatgcgctg aaaggtctgc
     1741 cttttcccat aatgtctcta ttaatgggta tatatgtacc tattaatatt gatacataca
     1801 agcgtttttc agtttatctt caactattta cgatatttgg gtacaaaact ttgaaaaaat
     1861 cagatcattt tgtcgatttt tataaactaa aagctgctgc ttagaagctt gcgaaactta
     1921 aatgattaat tatatctctg attacaaaga gaatcaccat ctcagggaaa gttttaacct
     1981 gttggcgaag caaactttcg gaatagattt cgagacttgg tatcaatacg gtttttatga
     2041 ccattcttat gtttgtcatt cttttgtgga aaacggccgt gttatttcca atgtttctgc
     2101 cacaaagatg cagttgatca tagacgggaa agacattaat gccattcaga ttgggacggt
     2161 aatgacctgc cccgaatgca gaatgtcggg actggccggc cggttaatgc atacggtaat
     2221 tgaggaatac gaaccgcaca attacatgat tattttattt ccaaacgaaa cggttatgga
     2281 tttctatccc aggttttctt ttcggttgac caacgacaca ctttattcca taaagacgaa
     2341 gtgctccggt gaagcctttg ataaattcag gaaactggat atcaaagaaa gtgccgggcg
     2401 ccgaattatt gagcaggcgg tgaagacaaa aatccctctc tcggaaagga tggatgtccg
     2461 ggcaaaccat gccatttgtt tatggcattg tctgaatagt ttccaggacg atatctacat
     2521 cgcggattcc ggactatgtg ttgtgatata caaaatgagc ggtgatcgca tagatctgta
     2581 cgatgtgatt actccgttta aaattttgat aggggatatt ctcccggcac tttccggagg
     2641 agaagaaaag tgtgtgggat tggggtaaac ccggtttgct gcgcaacaga cattttagca
     2701 gaattcatca agctgtaaaa cagcactttg acaaatagca aagaaggcgt tgcgcggtaa
     2761 accgggttta cccggatgat tttaagcttt ctcgattttt tctttctcca gcttttttgt
     2821 gcaaaagaac caatcataac atgttactcc ctcttcgggg atctccaggc ggtcttttgc
     2881 ctgaccgcag ctgccggctg tgggaacaat tacatcatcc cccgggcgcc agtcagctgg
     2941 cagggcaact ttgaactcgt cgaccgtctg aagtcctatg agtacacgtt tgatctcatc
     3001 aaaattacgg ccaagggata aaggataata aatgatggtg cgaataatgc ctttgggatc
     3061 gatgaagaaa acggcacgaa cggctttggt gttgctctct cccggttgaa ccatgccata
     3121 aaggttggcc acctccatgg tgatgtcttc aatgagcggg aacttcactt caatgttctt
     3181 cattcccttg tactggattt tctccttgat ggtccgcagc caggcaatgt ggctgtacaa
     3241 gccgtccacg gataatccta ccagttctgt attcaatgct ttgaattctt cctccatgga
     3301 ggcaaaggtc atgaactcag acgtacatac aggtgtgaag tctgcagggt ggctgaacag
     3361 gattacccat tttcctttgt agtcagacgg gaagttaatg tctccctgcg ttgtaatggc
     3421 tttgaactgt ggtgcaggtt ccccgattct ggggatagga tatgattttg ttgaactgtt
     3481 aacgttttcc atggtttttt gagttattta ttgtttttat ttctgtttac tgaagcaaag
     3541 gtaaggcggt caataatatt aatcaaacag attttattta tataattata gataatttct
     3601 atatctttgc tttatgacgt tacaacaact ccagtacatt gtcgccctgg accgtcacag
     3661 gcattttgtc aaagctgccg gtgaatgtgg catttcccag cccaccctga gcgccatgat
     3721 ttcccgtctt gaagaagaac tggatgtcag gatatttgac cgttccaaac accccgtaga
     3781 gccaacaccc atgggagaag agatccttca ccaggcgcga ctggtattaa aacaggccat
     3841 gcaggtgcag gagctggtac aggccgggaa agaaagcctg gatacccgtt tcagcctggg
     3901 tgtcattcca acagtggctc cctatattgt tcctcaattc ataggtttgt tcaaaaagga
     3961 ttttcccctg acccgggtca gtatcctgga aatgcggacc gatgaaatca tccgtgatct
     4021 gaaagaggca aaaattgaca tgggcctttt ggccactcct ctccttgatc cggacctgtt
     4081 ggaaattccg ctttattatg aaaaattcct ggcctacatt gctccttccg atcccaatta
     4141 ccctaaaaag gaactctcgg ccactgaact gcccctgcaa catttgtggg ttctgcagga
     4201 aggtcattgc ctgcgcaacc aggtattcaa tttttgtacg gataacaccc cggcagacaa
     4261 gcaggtgtac gaagcaggaa gtatagataa cctggtcagg atcatagaca ccaatggcgg
     4321 ttacaccctc attccgcaat tgcatctgcc catgctggat gaaatacagt ccacccatgt
     4381 acgcccggta aacaacccgc ccccggtacg tgaaatttcc attgccgtgt acaaagattt
     4441 ttaccgtgag aggttgttga atgccgttgc caacaccatt aaacagatca ttcccgcgga
     4501 tatgctcgac acaaggctca aaaaattcgc tatcagactc tagccgggtt caggccagaa
     4561 accctgcagc ccggtcaaaa actgaccgga aagaagtaat aagctccgtt tcgtggattt
     4621 tgccgttttc cggaaaaggc gactgatggg tgtattcata aggaaaatcc aattcccggg
     4681 tcatatatcc ggccagctcc tgccctgtgg cctcgtgcaa acccaccgtg ggaatcacag
     4741 tgtcctgccg cagggatatc atcaggatcc tgtcttttgc cccggcaaag aacatccggc
     4801 gaccgtcttc cggatcggcc gaacgtatca tcaaccggaa agcattggta agtttgtcag
     4861 aaacaccggc ctggcggcca tcctgcccct gctgaaaatt gtgaaattcg cccaggtagt
     4921 attccctgat cttctggtaa gcttcctggt ccatgatatc gcgggaattc ccgttcatcc
     4981 tttcaaagac agatcccccg caaaatgtga aaagcctgga gtccggcagg ttttcataag
     5041 gatcggccat cagtaccacc tgggcaagga gtgccccaat ggaataagca aaaaaatctg
     5101 tccggaccga ttctgtgaat agcgggtgcc tgccctgttt catgtcgtta ataagctgga
     5161 tcaggttgaa ggcagattcc aggcccgagc tgtaaaacct gagtgggctc acagacaggc
     5221 gcgaactcag ggctaggttg aaaaaagatg cattgcacaa atttttgatt tcctgttttc
     5281 tccggttcag ccaaggcaca agtccacgcg gtgtaaccca ggcagcaggc gttctgttca
     5341 tatgaaatgc aataggaaac aacacgaccg ggaccccgca ggtcatgacc aggtcttctg
     5401 cccaggttag gtatttattc catgaccttt cattcaatcc gtgaagcagg ataattgccc
     5461 tgtcaaaacg gtcagcgccg gccggggcaa aaataacata tttgaattgc tgattctcac
     5521 ctatggtgga atcggcagcc gggcaaaacg gggcactctg tatgcttttc tgcagttgtg
     5581 agacttcttc ccgtcccagt tcccgggtaa attcaaaggg aagggtaacc acaggcttgc
     5641 ccggcagtgt cacctcccgc tcaaaagaga agcatgagtc caaaagcttt gttcgttcca
     5701 tggttcaaaa caaaaccata aagctccata aagcaaataa atgggatgga tgacgggaat
     5761 gtgggataaa cagattgccc ctggggcgaa gcgcaccttt tccggggtaa aaaattttaa
     5821 ctttgcaaca tgagcttcat gcgaagaaaa atcccgccgt ttatctataa aaggtcaaac
     5881 cttaccctcg tggtggcctt tactgccctc tttgctctgt tgttcatcaa catatacaaa
     5941 cccttcgaat cccctcagtg gtatcccata acacaatttg aatatttcct gtattccagt
     6001 ctcattatcc tgaccggtgt cctggtggtg gtgatcagcc ggattattat gtattattat
     6061 acaaaaaaga gaacgctcac ctattgggaa tatctcatat gggtcattgc cgagatcttt
     6121 ttcatgtcac tcttctacac cctttactcc tataccctgg acgaaacgcg agatttcacc
     6181 gaagtttacc gttcttctat aatcaagacc agcctgatcc tgttgcttcc ctacatcata
     6241 tccctgttgt tcctttcctg gcgggagaaa tccatgcgcc tgaaggagct cgaggtaaag
     6301 ggacaggaag aaggagtgcc tgccagaacg gagatcatca atttccggga tgaaaagggt
     6361 gtgttgcagc tttccataaa aaaagactcc ctgctgtacc ttgaatcagc cgataattac
     6421 gtgtctgtct ggtatttgtc aaaatcagga gtcagcaatt acctggttag gaatacactt
     6481 aaagaaatgg aattgcgttt ttcccaaacc aggatcgtgc gctgtcaccg ttcctacctg
     6541 gtcaacctgg accaggtaaa ggttgccaga cgaacttcca atggaatcgt tttggacctg
     6601 ggcacaccaa aaataccgga tatacctgtc tcccgcacgc atgcagagca gattgcccgt
     6661 tggttcacca acatgtagcg accttatttc tttttaagca ggtcgcgaat ctctgtcaaa
     6721 aggacttctt cgcgggtggg ttcgggctct ggagccggtg ctcccggtgc ttcttctttc
     6781 tttttcgccg aggtcagttt gtttagcccc ttgatgagca ggaacacgca gaaagcaatg
     6841 atgatgaaat caatgagcac ctgtatgaag ttcccgtaat tcagggttac agcaggcagc
     6901 atgacattag gatccaggat gctggcacgt tcccccacta ttgtccactt ccagtcggta
     6961 aaattaactc ctccgacaag ggccccgacc aggggcatga gcaaatccga gaccagggaa
     7021 gatacgattt tcccgaaagc cgcaccaata atgataccta ccgccatatc caccacattg
     7081 ccccggacgg caaatgcttt gaattcttta agaaattttg acataatggt gttattaaaa
     7141 ttcaaataaa tatacaaaga tttatgcctc tttaaacaac ttttataact ttacggacag
     7201 acacttttta attaatacat attatgaaat gtagacttat tataaccgca ttactgttca
     7261 ctaccctgac atcccttgct gctcgggacc cggaaggacg cctgctccga ttccctgcca
     7321 caggcaacgg gctcatagca ttctcttatg caggagacct ttatactgtg ccgcttgaag
     7381 ggggaaatgc ctcaagaatc acatcacaca atggatatga agtttttccc aaattttcac
     7441 ccgatggccg taccattgcc tttaccgcac aatacgacgg aaacacggaa gtctatacca
     7501 ttcctgcaga aggaggcgaa ccaaaacgtc tgacctacac ggcaaccctg gacagggaca
     7561 atataggaga ccgcatgggt cccaacaaca ttgtcatggg atggaccacg gatggaaaaa
     7621 acgtggtgtt tcgttcccgt tggtatagct tttccggcat gcgcgcccac ctgttcacgg
     7681 ttcctgccgt aggcggtgaa ctaagccaga tcccaacctc cgagggcgga ttctgttctt
     7741 attccccaga tggtcagtac ctggccctga accgcatgtt ccgggagttc aggacctgga
     7801 agtattacaa aggaggacag gcagatgaca tctgggtgaa caagctggga actacacagc
     7861 tggaaaacat cacggccggc gaaggtgaca acgcccagga tatctttccc atgtggattg
     7921 gcacagatat atattttatc tctgaccgcg actcgatcat gaaccttttc cggtacgaca
     7981 cgggcacaaa aaagactgaa aaaatgaccc gtttcgatac gtacgacata aaattcccgt
     8041 cagcctgggg ggaatacata gtctttgaaa acggtgggta catctattcg ttcaatacaa
     8101 gaaacaggga actcaataag gttgagatca ccctgcatga agaaaacctc catgccagaa
     8161 cacggctgat ggatgtatcg gcccaggctg ccaattactc cctgtcaccc aatggcgaac
     8221 gggtgctcgt cacttcccgg ggcgatgtgt tttc
//
LOCUS       DAOSOF010000085         8244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8644_length_8244_cov_4.999756, whole genome shotgun sequence.
ACCESSION   DAOSOF010000085 DAOSOF010000000
VERSION     DAOSOF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8644_length_8244_cov_4.999756"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="dnaN"
                     /locus_tag="PLM68_05835"
     CDS             <1..825
                     /gene="dnaN"
                     /locus_tag="PLM68_05835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT54920.1"
                     /translation="TWTTGKFQVPAFPAEDYPVAKEPNDNLSITFSPDVLLEGINRTL
                     YATGDEELRPVMNGIFFDLKTNGANLVASDSYKLVCYTRSDVKSSEDASFILPKKPAN
                     ILRNLLVKMEDDVTISFDKTNAFFNFGSYRMTCRLVEGNYPAYRSVIPQNNPNNIIAD
                     RIELMNAVRRISVCANQATNLIRLLFSANQIFVSAQDVEFRIAAQERISCQYDGPAIE
                     MGFKSVFLAEILANLPGTEVNIALSDPSRVGLITPIYTEPENEELCALLMPMMITQ"
     gene            1019..1780
                     /locus_tag="PLM68_05840"
     CDS             1019..1780
                     /locus_tag="PLM68_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT54921.1"
                     /translation="MKLNLKRPILFLDLETTGLNVATDRIIEICVLKLSPDGTREIKT
                     RRINPTIPISPEAQAIHGISNEDVANEPTFKEIAKSLAKWMEGCDIAGYNSTKFDVPM
                     LSEEFLRAGIDFDFRKRKLIDVQNIFHKLEQRTLKAAYQFYCDKNLDNAHSAEGDTIA
                     TMEILEAQLDRYPDDLKNEIGFLSEFSTRNRNLDYAGRIVLDDKEQPVFNFGKHKGRS
                     VKEVLHTEPSYYDWMMKGDFTLDTKKVLTEIKLSL"
     gene            complement(1892..3376)
                     /locus_tag="PLM68_05845"
     CDS             complement(1892..3376)
                     /locus_tag="PLM68_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT54922.1"
                     /translation="MKEVIVIGAGPAGMLAAGTAAEKGARVVLLEKMEKPGRKLAITG
                     KGRCNITNLKNWEDFSPHVHPVNRYFKPAFYNFTSEDTVTFFNNIGLPTVLERGTRVY
                     PASQRAFDVIDALKSWLERLGVRMICGCQVLEFLTEAAPTSGDQETTSCANFPVSNAT
                     ASSAAPTSGALEIKASASAPINTKKAGTPTRIKAVRVRNSRGEERLLTADHFILATGG
                     KTYPGTGSTGDGYALASALGHTIEPLFPTLTALMPVDYDRRLEGVELRNVRLDLYIEE
                     VLRRSEEGELLFSNLGIEGPIGFRVSRQAVVALNKGQRVSLHLNLKPALTREQLMARW
                     KRERENSLVGSGSVSVPGSGMSARNSVGAGNGSPGKNSVGPGNGSEADLLKRLRAYMP
                     ETLITPFIKYQTVSGLHPVDAMTKWVFPIASYGDWARAVVTAGGISMKEVDIRTMRSK
                     LVENLSFAGEVLDLDADSGGYNLQIAFATGRLAGGGTSCAKNQQ"
     gene            3611..5359
                     /locus_tag="PLM68_05850"
     CDS             3611..5359
                     /locus_tag="PLM68_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOT54923.1"
                     /translation="MKKNMFMPAASNLHALAGVIDPATKKRSPSEVRLSSGKRMSSGI
                     MLFSGKRMSLLSSCRELSSGILLFTCFLLLFTACNRQERLPRIAIAGIAIECSTFSPA
                     KTVEEDFSISKAEEVFSRYDFMAPDSVLRQKAHWLPAMYSRATPGGIVTRKTYESLVN
                     QTLEELRKNLPYDALYFDIHGAMSVEGLDDPEGDLILRIKEVIGNKPIISTCMDLHGN
                     VSQRLAENTDLITCYRMAPHEDAMESKRRAVANLLERLETGKGRPACKAWVKVPILLP
                     GEKTSTRVEPAKSLYAKVAPAAAREGVIDAAIWIGYAWADEPRNHAVVMVTGDDENAV
                     ETTAAELARAFWNVRHEFDFIAPTASLDECLEKALASDKKPFMISDMGDNPTAGGAGD
                     VTWTLARLLERPEFRSSGGMSTSQSASSSPDLIYASIPGPKMVEKALAAGVGAKVEET
                     VGAMVDDRYQPPVLLSGTVEFIRESDTNTEVVIKTGSMHVIVTQKRKPYHYESDFENL
                     GFKPRETDIIMIKVGYLPESVYNMRGDWMMALTRGGVDQNLLELPYQRIERPMYPLDP
                     DMADPPLDVKMWKLKF"
     gene            5379..5451
                     /locus_tag="PLM68_05855"
     tRNA            5379..5451
                     /locus_tag="PLM68_05855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5412..5414,aa:Thr,seq:cgt)
     gene            5467..6639
                     /locus_tag="PLM68_05860"
     CDS             5467..6639
                     /locus_tag="PLM68_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT54924.1"
                     /translation="MVKPRMIRGSLIGTCVLLCTLAFQSCPTNRSKSFRKHPLEEYGL
                     SEGDIDYIYKPRYANNFYIVREEGEKVLYLTDPVTYNTTRHVVNKVRNVVCLSSTHIA
                     YLDAINETSSIVGVSGAQYIYNKNIRKAVDLGYESAIDYEALLQLKPDIVFAYAIHGE
                     SADYIEKIKQLGLNVVQIPEYLENHPLGKAEFLVAFASFFDKELMAIEQFSKISHTYL
                     EYCKLAKETVTTQNPAESANGGFEPVKVLVNAPFKDVWYVPGTDSHLQILVKDAGGMI
                     LGSKPGVKTTAMSVEQAYLYAQQADFWINTNQYEDLASLARLDPRFAEAPAFRNGNVW
                     NNNLRSTPEGGSDYFESGAVNPHLILADLIAIFYPQALPKHMFNYYRKVGSSEVEP"
     gene            complement(6699..>8244)
                     /locus_tag="PLM68_05865"
     CDS             complement(6699..>8244)
                     /locus_tag="PLM68_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HOT54925.1"
                     /translation="GALPAGAQPQQAQEQLAGQGVPQVQEPVHEERSVLDVVADSLLA
                     GNKVAVMTGSLGEIMLSEGYPEVIIQCFDDIMDGFAALHAGKVSYLMTAYTTAYVVSR
                     SNPSYRILPYHLTNEGAGVAVAKDNSTGLLRQIDSVLAGFRQDGTLDKIIGNWIRHDG
                     SEYVVTEFDNKGTSSGAGALPPLRVGTSANREPMCFVRNNTISGLDWELMMRIGQALG
                     REVVFVDMKYAALIAALQSGKVDAVASNMTATAERKKQVDFSAVYYVNPQVLVEWNPV
                     GAEATAARSSGQSFWEKFTDSLYRNLLHEKRYMLILDGLWVTILIAVMSVIFGSIAGA
                     GVCALRMSRHTVLRAFAKSYITLFRAIPQVVILMVMFYVVFATARISGIFVSVLTFSL
                     IFASYSSELFRTSLEAIPLGQRQAGYAMGFNKYQTFRYILLPQAARIAFPVYKGEIIS
                     LIKMTAIVGYIAVQDLTKAGDIIRSRTFDAFFPLVMVALIYMLIIWGFTKLLSVRRWN
                     LSAPKS"
BASE COUNT         2193 a         2252 c         1904 g         1895 t
ORIGIN      
        1 acctggacga ccggtaaatt ccaggttccc gcatttccgg cagaagatta ccctgtagcc
       61 aaagaaccca atgacaacct gtccatcaca ttctccccgg acgtcttgct ggaaggtata
      121 aaccgcaccc tttatgcaac aggtgacgag gagttgcgcc ctgtgatgaa cggcattttc
      181 tttgacctga aaaccaacgg tgccaacctg gtggcctcgg attcctacaa actggtttgc
      241 tacactagat ccgatgtcaa atcctccgaa gatgcctctt tcatcctccc caaaaaaccg
      301 gccaacatac tcaggaacct tcttgtcaag atggaagacg atgtcaccat atccttcgat
      361 aaaaccaacg ccttcttcaa cttcggttcc taccgcatga catgccgcct ggtggaagga
      421 aactaccctg catacaggag tgtcattccc cagaacaatc ccaacaacat catcgcggac
      481 cggattgaac tgatgaacgc ggtacgtcgc ataagcgtat gcgccaacca ggcaaccaac
      541 ctcatcagac tgctcttttc tgccaaccag atattcgtct cggcccagga tgtcgaattc
      601 cgcattgccg cccaggaacg catctcctgc cagtacgacg gtccagccat tgagatggga
      661 ttcaaatctg tgtttctggc ggagatcctg gccaaccttc cgggtaccga ggttaacatt
      721 gccctgagcg acccctcacg cgtaggcctc atcaccccca tttacacaga accggaaaac
      781 gaggaactct gcgccctgct catgcctatg atgatcaccc agtagagtgc cgcgcacttt
      841 tgatacattg acctatataa cagatagtta acaaaagagc tcccccagag agctcttttg
      901 ttatatcact gtaagccaat ctcatacatc aaaagtgcgc ggcactattt tcccgtcctt
      961 ggcaaggaga aactccatta atttttgtac ttttgtgact cataaagccc aaatcaacat
     1021 gaaactcaac cttaaaagac ccatcctgtt cctggacctg gaaacgaccg gcctgaatgt
     1081 ggcaactgac aggatcattg aaatttgcgt tctaaaactg agtcccgacg gaacacgcga
     1141 gatcaagacg cgccggatca atccaaccat acccatatcg cctgaagcac aggcaataca
     1201 tggtatctcg aacgaagatg ttgccaatga acccacgttc aaggaaattg ccaaatcact
     1261 ggccaaatgg atggaaggct gcgacatagc gggctataat tctaccaaat tcgatgtgcc
     1321 catgttgtct gaagagttcc tgcgggcggg aatcgacttc gatttccgca aacgcaaact
     1381 aattgacgta cagaatattt tccataagct cgagcaaaga acgctgaaag cagcttacca
     1441 attctactgc gataagaatc tggataatgc tcacagtgcc gaaggcgata ccattgcaac
     1501 catggaaatc cttgaagccc agttggacag atatcccgat gatcttaaga acgaaatagg
     1561 cttcctgtct gaattctcta ccagaaaccg caacctggat tatgccggcc gcatagtgct
     1621 cgatgacaag gaacaaccgg ttttcaactt cggcaagcac aaaggccgta gcgtaaaaga
     1681 ggtactgcat acagaaccct cctactacga ctggatgatg aaaggagatt ttacccttga
     1741 taccaagaaa gtcctgactg aaatcaaatt gagtctatag gttgtcccga tcccgttcgc
     1801 actcacctct ggcgcatatg aggtggaact tcccgcgcca tttttaactc taaatattaa
     1861 taaaaccttg ttttactgtt aaatagaaat tttattgctg atttttggcg catgaggttc
     1921 cacctcctgc cagcctgccc gtagcaaacg ctatttgcag gttatacccg ccactatctg
     1981 catcaagatc cagcacctca cctgcaaacg acaggttttc aaccagtttt gaacgcatcg
     2041 tacgtatatc cacttctttc atagagatac caccagccgt cacaacagcc cgtgcccagt
     2101 ctccgtaaga agctatggga aagacccatt ttgtcatggc atccacagga tgcaagccgc
     2161 tcacagtctg atattttata aaaggggtta tcaatgtttc cggcatatat gccctaagcc
     2221 gctttagcag atcagcttca gatccattcc ccggaccaac agaattcttt cccgggctcc
     2281 cattcccggc cccaaccgaa ttccttgccg acatcccaga cccaggcaca gacacagaac
     2341 cagaaccgac aagagaattt tcccgttccc gtttccaccg ggccatcagc tgttcacgtg
     2401 tgagtgcagg cttcaggttt agatgcaggg acacgcgctg ccctttgttc agagccacca
     2461 ctgcctgcct gctcacgcga aacccaatag gaccttcaat ccccaggtta ctgaaaagca
     2521 gttctccctc ctcggatcgt cgcagcactt cctctatgta cagatccagc cggacattgc
     2581 gcaactccac cccctcaagc cgccggtcgt aatccactgg catcagcgcg gttagcgtag
     2641 gaaagagtgg ctcaatggtg tggccgagcg ccgaggccag tgcatagccg tcccctgtcg
     2701 aaccggtacc gggataagtc ttaccgccgg tggccagaat aaaatggtcc gcagtcagca
     2761 gcctttcttc accccgcgaa ttcctgactc tcaccgcttt gatccgtgtg ggtgtacctg
     2821 ctttttttgt attgatcggg gcacttgccg aggcttttat ttccagagct ccggaagtgg
     2881 gtgctgcact gcttgcggta gcattgctga caggaaagtt tgcacaggaa gttgtttcct
     2941 ggtctccgga agttggtgcc gcttccgtca ggaattccag cacctggcaa ccgcagatca
     3001 tgcggacacc caaccgctca agccaggatt tgagagcatc tatcacatca aatgcccttt
     3061 gagaagcggg gtacaccctt gttcctcgct ccaataccgt tggcaatcca atattattaa
     3121 agaaagtcac cgtatcctcg ctggtgaaat tataaaatgc cggcttgaaa taacggttca
     3181 ccgggtgtac gtgcgggctg aagtcttccc agttcttgag gttggtgata ttgcagcggc
     3241 cctttcccgt tatggccagt ttgcgacccg gtttctccat cttttctagt aaaaccaccc
     3301 gggcgccttt ttcggcagcc gttccggcag ccagcatacc tgcgggtccc gccccaatta
     3361 caatcacttc cttcataatg caaaagttac tatgtgccgt tcacactttt ccgccggacg
     3421 attcatgaac actacctcca cactgaaaaa aaagctatgt actgtttata cttcactgca
     3481 ggataattca tgatcacttc cttcatactg caaagataga actaagcgtt gatataagtg
     3541 aattgccggg actgataaaa atccttacat ttgtatgtaa taattcgcgt cgaacaaatc
     3601 attatctgta atgaaaaaaa atatgttcat gcctgcagcc tctaacttgc atgcgcttgc
     3661 cggtgttatt gatcctgcaa ctaagaaacg ttcgccttca gaggtacgtt tgtcttctgg
     3721 aaaacggatg tcttcaggaa tcatgttgtt ttcggggaaa cggatgtcct tgttgtcttc
     3781 ttgtagagag ctgtcttcgg ggatccttct atttacgtgc tttctgttgc tatttactgc
     3841 gtgcaacagg caggagcggc tgcccaggat agcgatagcc gggattgcca tagaatgcag
     3901 tacgttttcc cctgcaaaga cagttgaaga ggatttcagc atcagcaagg cagaagaggt
     3961 gttcagccgg tacgatttca tggcccctga ctctgtttta cgccagaaag cacactggct
     4021 accggccatg tattccaggg ccactcccgg cgggatcgta accaggaaaa catatgaatc
     4081 gcttgtaaac caaacacttg aggaactcag gaagaacctt ccctacgatg ccctgtactt
     4141 tgacatccat ggagccatga gcgtagaagg actggatgac ccggaaggag acctgattct
     4201 gaggatcaag gaagtaattg gcaacaagcc tatcatatcc acctgtatgg acctgcacgg
     4261 gaacgtatcc caaagactgg cagaaaacac agacctgatt acctgctacc gcatggctcc
     4321 gcacgaagac gccatggaat ccaaaaggcg ggcagtggcc aacctgctgg aacggctgga
     4381 aaccggcaaa ggaaggcctg cctgcaaagc ctgggtgaaa gttcctatcc tgttgcccgg
     4441 tgaaaagaca agcacacgcg tagaaccggc aaaatccctt tacgcaaagg tggctcctgc
     4501 agcagctcgt gaaggcgtca tagatgcagc catctggatt ggatacgcct gggccgatga
     4561 accccgcaac cacgctgtgg tcatggttac cggtgatgac gaaaacgcgg ttgaaacaac
     4621 ggccgcagaa cttgcccggg ccttctggaa tgtcagacat gagttcgatt tcattgctcc
     4681 taccgcctcg ctcgacgaat gcctggaaaa agcactggca agtgacaaga agccctttat
     4741 gatcagcgac atgggagaca atcccaccgc cggaggtgct ggtgacgtca cctggactct
     4801 tgcccggctt ttggaaagac ccgagttccg ctcttccgga ggcatgagca cgtcccaaag
     4861 tgcaagctcg tccccggatc ttatatatgc ctccatcccc ggaccaaaaa tggtggaaaa
     4921 agcattggcg gcaggtgttg gcgccaaagt ggaagaaact gtgggagcca tggtggatga
     4981 ccgttaccag ccgccggtgc tgctcagcgg aactgttgaa ttcataaggg aaagcgacac
     5041 aaatacagag gtggtgataa aaaccggcag catgcacgtg atcgtgacgc aaaagcgtaa
     5101 accctaccac tacgaaagcg atttcgaaaa cctcggcttc aagccacgcg agacagacat
     5161 cataatgatc aaggtcggtt acctgcctga aagtgtttac aatatgcggg gtgactggat
     5221 gatggcactt acgcggggcg gcgtggacca gaacctgctg gaactcccct accagcgcat
     5281 agaaaggccc atgtatccgc tcgacccgga catggccgat cctccgctgg atgtgaagat
     5341 gtggaaatta aaattttaat ttatctttgc agcccattgc cttcttagct cagctggtag
     5401 agcagctcat tcgtaatgag caggtcgcgg gttcgagtcc cgtggaaggc tcaaaaacaa
     5461 caaattgtgg ttaaaccaag aatgattagg gggagcctga ttggcacctg tgtgttgctt
     5521 tgcacacttg cttttcagtc atgccccaca aacagaagca agtcttttcg taaacatccg
     5581 ctggaagaat acggtctgtc agaaggagac attgactaca tctacaaacc acggtatgca
     5641 aacaattttt atattgtcag ggaagaagga gagaaagttc tctatctgac cgacccggtt
     5701 acatataaca cgacacgtca cgttgtcaac aaggtacgta atgtcgtatg cctttcctcc
     5761 acacacatcg cttacctgga tgcaatcaat gaaacaagct ccatcgtggg cgtttcgggt
     5821 gcgcaataca tatacaacaa gaatatccgc aaggctgtag acctgggata cgaatcggct
     5881 atagattacg aagccctgct gcaacttaaa cccgatattg tctttgcgta tgccattcac
     5941 ggtgaatctg cagactacat agaaaaaata aaacagctgg ggctaaatgt tgttcaaatc
     6001 ccagaatacc ttgaaaatca ccctcttgga aaagccgagt tcctggtggc gtttgcttct
     6061 ttctttgaca aggaactcat ggcaatcgaa caattcagta agataagcca tacttacctg
     6121 gaatattgca agctggcaaa agaaaccgtc acaacgcaaa atcctgcaga atctgccaac
     6181 ggcggttttg aaccggtcaa agtcctggta aacgcaccct tcaaagacgt gtggtacgta
     6241 ccgggaaccg acagccacct tcaaatcctg gtcaaagatg ccggaggcat gatcctgggc
     6301 tcgaaacccg gcgtcaaaac aacagccatg agcgttgaac aggcatacct gtacgcccaa
     6361 caggccgatt tctggatcaa caccaaccaa tacgaggacc ttgcttccct cgcccggttg
     6421 gatccccgct ttgccgaggc tcccgccttc cgcaacggga acgtctggaa caacaacctg
     6481 cgcagcacgc cggaaggcgg gagcgattat ttcgaatccg gagccgtcaa cccgcacctg
     6541 atcctggccg atctgatagc catcttttac ccccaggcgc tgccaaaaca catgttcaac
     6601 tactacagga aggtcggaag ctctgaggtg gaaccttagt gcgccaaatt cagtggtatt
     6661 tttctattta ggtgattttc tgctggttat gaagactatc agctttttgg cgcactaagg
     6721 ttccacctcc ggacgctcaa cagcttcgta aatccccaaa taataagcat atagataagc
     6781 gccaccatga caagggggaa gaaggcgtca aatgtgcggc tgcggatgat gtcgcctgct
     6841 ttggtcaggt cctggacggc aatataccct acgattgccg tcatctttat cagtgatatg
     6901 atctctccct tatatacggg aaatgcaata cgggctgcct gtggcaggag gatataccta
     6961 aaggtctgat acttgttgaa tcccatggca tagcctgcct gacgctgtcc caaaggtatc
     7021 gcttccaaag atgtacgaaa gagctccgat gaatacgaag caaagatcag ggaaaaggtc
     7081 agtacagaaa caaaaatccc gctgatccgg gccgtggcaa ataccacata aaacatcacc
     7141 atcaggataa ccacctgcgg gatcgcacga aagagggtga tatagctttt ggcaaaagcg
     7201 cgtaaaaccg tatggcgaga catcctcagg gcacaaactc ctgcccctgc aatggaacca
     7261 aagatcacag acatcacagc aatcagaatg gtgacccaca acccgtccag aatcagcatg
     7321 tacctctttt catgcaacaa attacggtac agcgaatcag tgaatttttc ccagaaactt
     7381 tgcccggaac tccttgctgc agtagcctcg gctcccacag gattccattc aacaagtacc
     7441 tgaggattaa cataatatac agctgaaaaa tcaacctgtt tcttgcgctc tgccgttgcc
     7501 gtcatattgg atgccaccgc atctaccttt cccgattgta aagcggcaat aagggccgca
     7561 tacttcatgt ccacgaatac cacctctctg cctaaagcct gcccaatgcg catcattaat
     7621 tcccagtcaa ggcccgagat ggtgttgttg cgcacaaagc acatgggttc ccggtttgca
     7681 gaggttccca cgcgcaaagg cggcaatgcg cctgccccgg atgatgtgcc cttgttgtca
     7741 aactctgtga ccacatattc agaaccatca tgccgtatcc agtttccgat gatcttatcc
     7801 agtgtgccgt cctgcctgaa tcccgcaagg acagagtcaa tctgccggag cagacctgtg
     7861 gaattgtcct ttgccacggc cacaccggct ccttcgttgg tcagatggta aggcagtatc
     7921 ctgtaggagg gattagaccg tgaaacgaca taagccgtgg tgtatgcagt catcagataa
     7981 ctgactttcc cggcatgcaa ggcagcaaat ccatccatga tatcatcaaa acactggatg
     8041 atcacctctg gatacccttc gctgagcatg atctcgccca gcgacccggt catcacggca
     8101 actttattgc ccgccagcaa gctgtctgcc accacatcca acacagaacg ctcctcatgt
     8161 accggctcct gaacctgcgg aacgccctgc cctgccagct gctcctgagc ctgttgtggc
     8221 tgggctcccg cgggcaaagc cccg
//
LOCUS       DAOSOF010000086         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8672_length_8222_cov_5.287254, whole genome shotgun sequence.
ACCESSION   DAOSOF010000086 DAOSOF010000000
VERSION     DAOSOF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8672_length_8222_cov_5.287254"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PLM68_05870"
     CDS             complement(<1..1726)
                     /locus_tag="PLM68_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT54926.1"
                     /translation="MITKEFLNPKSIVVVGGSNDLHKPGGKVIKNLKDTRYPGDIYIV
                     NPKADTIQELTSYHTVEEIPGAEVAILAIPAALCLHAVETLCSTKGTKGVIIFSAGFS
                     EESHEGAVLEKKIRDVADKHGATLIGPNCVGYMNEHYAGVFTSPIPRFVPDSIDLISG
                     SGATALFIIDAAVQLGIPFANVFSVGNSAQTGLEEALEYLDQSYVHGKSAPVKLIYAE
                     TIKNPLKLWKHAASLYRKGARIAAVKAGSSEAGSRAASSHTGALASPDTAVAALFEKA
                     GIIRCNGRNQLLTVACILLKGAPAGKNMCIITHAGGPAVMLTDVLSENGIAVPTLEEG
                     KRKKLLAKLFAGSSAANPIDFLATGTAGQLGEIIEFVENECPEFDGMAVIFGSPGLNS
                     VFDVYDLIHQKMSECTKPIYPIFPSTLNVLEEIREFQKKGRLYFPDEVIFGSALAKVL
                     NTPVPVPDNIALPAIDEQAVHRLVDKAGEGYLSPAQVSDLLDAAGIPRAQEAVAHNEE
                     ELGAAAARVGFPLVMKVIGPVHKSDVGGVRLNIRDEHALREAFREMITIKDTTAILLQ
                     SQLTGRELF"
     gene            1809..2660
                     /locus_tag="PLM68_05875"
     CDS             1809..2660
                     /locus_tag="PLM68_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOT54927.1"
                     /translation="MILIADSGSTKVAWRVLHKDGTIQAIQTIGINPFFVSEPDILRT
                     LEENLKPAVRGDVSQVFFYGAGVAGKDKIDILTRCFQTAFPGCSVEANSDLLAAARCL
                     FGSEKGIAAILGTGANTCFYDGEKIVDNVPACGYILGDEGGGAVLGKKLVSDYLKRML
                     PADLSEAFEKQYKLGLLDIIEKVYRQPLPNRFLASFALFLGDHQSHPYVQEFLGKAFD
                     EFFSRNIIHYDYKTYPVNIVGSVAYYFRDEVMASAARHGMKIGKIVRTPIEGLVAYHR
                     NLMTQQE"
     gene            2657..3481
                     /gene="murQ"
                     /locus_tag="PLM68_05880"
     CDS             2657..3481
                     /gene="murQ"
                     /locus_tag="PLM68_05880"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOT54928.1"
                     /translation="MIQKVTEQRSRYDHLEKMSVRELLENINREDQTVPLAVIPCIPQ
                     IEKLVEEIVTRMKEGGRLFYMGAGTSGRLGILDASEIPPTFGAPYDLVIGLIAGGDAA
                     IRRAVEKAEDSLEAGWEELRVHHVGTNDVVVGIAASGTTPYVVGALREARKMGLLTAC
                     ITCNPGTPVTLEAEIPIVAVVGPEFVTGSTRMKSGTAQKLILNMISTTVMIRLGHVIG
                     NKMVDMQLSNAKLIDRGTKMIMEDTGIEDYDKARSLLLQYGTVRSAVNAVNDKSHV"
     gene            3462..4091
                     /gene="recR"
                     /locus_tag="PLM68_05885"
     CDS             3462..4091
                     /gene="recR"
                     /locus_tag="PLM68_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT54929.1"
                     /translation="MIRAMFEQRYVSDLLQKAVEAFSRLPGIGPKSALRLALYLLRQE
                     EEESENLAQSIVELRRKVVYCSECRMISDAALCPVCSNPERDRGIICVVENVRDVMSI
                     EHTGRYKGLYHVLGGIISPIEGIGPTDLSIEPLMDRLARGVVREVILALSTTMEGETT
                     CFYLYKKMASLDLNVTTIARGVGFGDQLEYTDELTLGRSIENRLPFKPE"
     gene            4105..5613
                     /locus_tag="PLM68_05890"
     CDS             4105..5613
                     /locus_tag="PLM68_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT54930.1"
                     /translation="MEHTFPLSWLLTAFAVYTALLFFVSWLTSRKADSNTFFRGNKKS
                     PWPIVAYGMVGTSISGVTFISVPGNVMNENFYYMPLVLGFVIGYLVIAKVLLPLYYKM
                     NVTSIYTYLENRFGFTTYRTGAAVFMVSRVLGAAVRIFIVVLVLHTFLPAGSVPFWIV
                     AGVFMLLIFLYTYRGGVKTIIWTDVLQTTFMLLAVVLTVISIAKAMDWDFRQMAGAVT
                     SSDYSDWFNWDWSASNNAVKQFISGIFITIVMTGLDQEMMQKNLSCKNLQSAQKNIYT
                     TSITLVVVNILFLTLGAVMAIYVNQHLGGIQGIGLADAAGSFTKAQTDKLFPVIASQY
                     LGLGAGLFFIIGLISASYPSAGGALTSLTTSFCIDFLGFNKKKEMPQERKDKIRKVIH
                     ASFAMLFFLIILVLYVVNNQAIINLVYLLAAYTYGPLLGFFFFGILTKYRVRDRVTPY
                     IALAAPALCYLADLALKTWFDFGLGFTVLIVNGLLVFFGMWVFREKTLREGA"
     gene            5858..6814
                     /locus_tag="PLM68_05895"
     CDS             5858..6814
                     /locus_tag="PLM68_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOT54931.1"
                     /translation="MIEIFYKSKGQILNDTDVRILDKLGLDDVLWIDLHDPTGDEKRA
                     AEDFLDTTLQSRAQAEEIESSSRYSETETTIFANTNFLIPGPDDYTSIPVSFILSESI
                     LVTLRHTPLRTLAEVQRKLLNNLKLYPTGYHIMVSILENRIDVDADLVEILSKEITQL
                     SRRIDLGESMDETFLLDVNQLQENTMVIRTNGVDKQRILSGIFKSDKFPRDLYTKLEI
                     LNKDIDSLIHHANFSFERLEYLQNTALGLINLEQNKLMRVFTFVSLLLMPPTLIASIY
                     GMNVKLPIVSGSQLWDFIILLLLMLLSVVIAIFLFKRRKMIK"
     gene            complement(6807..>8222)
                     /locus_tag="PLM68_05900"
     CDS             complement(6807..>8222)
                     /locus_tag="PLM68_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOT54932.1"
                     /translation="ASLQANAGKVVNTPSFPDTLWVDRPALPLSPVFILETQYSGRST
                     EEKLTWLREQLSSQGCSHILLGGLDQVAWLFNIRSRDIPYNPVAVSYALVDSKNATLF
                     ITPGKVGDNVTRLLREQGVGIAPYEAVTSVLEGLPLESVFMADGDTLNYSLFSLLLSR
                     FGSTGVKDMPSPVVLAKAVKNPVETEGFRKAFLYDGLAMERFFFWLEKSLEQDVLLTE
                     KDVAKKLSWMRSLSPDSMGDSFQYIPAYGANAALPHYNYLAGPGSRLEKKGLFLIDSG
                     GHYLYGTTDITRTVPLGPLTPLEREDYTIALKGMIALSRAVFLKGTKGTHLDMLAKDP
                     LWRHLRNYGHGTGHGIGHFLCVHEGPQDIRQTWKDQDLLPGMVTSNEPGIYREGSHGV
                     RHENILLCKELEENGFGSWLGFETLTLCHIDTSAILVSLMERGEIEWLNRYNRRVYEK
                     MAPFLTYEESRWLQEKTAPLI"
BASE COUNT         1982 a         2035 c         2139 g         2066 t
ORIGIN      
        1 tgaaaagctc cctgccggtg agttgtgact ggagcaggat ggcggtggta tcctttatcg
       61 ttatcatttc ccggaacgct tccctgagtg cgtgttcatc acggatgttc agacggaccc
      121 cgcccacatc cgatttatgc accggaccaa tgactttcat caccagggga aatcccaccc
      181 gtgccgcggc tgcacccagt tcctcttcat tgtgcgcaac ggcctcctgt gccctgggaa
      241 tgcctgcggc atccagcagg tcgcttacct gggccgggga caggtatcct tctcccgctt
      301 tgtccaccag cctgtggact gcctgttcgt cgattgccgg cagggcaatg ttatcaggaa
      361 ccggcacagg ggtgttcagt actttggcga gcgccgagcc aaaaatgacc tcgtccggga
      421 aatagagcct tcctttcttc tggaattccc tgatctcttc cagcacattc aatgtggagg
      481 gaaatatggg gtagatgggt ttggtgcatt cggacatctt ctgatggatc aggtcataga
      541 catcgaatac actgttcagg cccggtgaac caaagatgac agccatgccg tcgaattcgg
      601 ggcactcgtt ctccacgaat tctatgatct ctcccaattg ccctgctgtt cctgttgcca
      661 ggaaatctat gggattggcc gctgaagatc cggcgaacag cttggccagc aacttcttcc
      721 ttttgccttc ttccagcgtg ggaacggcaa ttccgttctc tgacaggacg tcggtcagca
      781 tgaccgcagg gcctccggca tgtgtgatga tgcacatgtt tttccctgca ggagcgcctt
      841 tgagcaggat gcacgctacg gtcaggagct gattcctgcc attacagcgg atgatgccgg
      901 ctttttcaaa aagggcggca acggccgtgt cgggactggc cagggcacct gtatgtgatg
      961 aggccgcacg gctcccggct tccgaggatc ctgcttttac ggcagctatc ctggcgcctt
     1021 ttctgtacag ggaggcggca tgtttccaca atttgagagg attctttatg gtttctgcat
     1081 agatcagctt cacaggcgcg cttttgccat gcacgtaact ttggtccaga tattcaagcg
     1141 cttcttccag gccggtttgg gcagagttgc ctaccgagaa gacattggca aatggaatgc
     1201 ccagctgtac tgctgcatct atgatgaaaa gggcagtcgc cccggagcct gatatgaggt
     1261 caatgctatc cgggacgaag cggggtatgg gtgaggtgaa aacaccggca taatgctcgt
     1321 tcatataccc cacgcaattg ggacctataa gggttgcccc gtgtttgtcc gccacgtccc
     1381 ggatcttttt ctcaagtacc gccccttcat ggctctcttc cgagaaaccg gccgaaaaga
     1441 tgatgacccc ctttgtgcct tttgtggagc agagcgtttc tacagcatgg agacataagg
     1501 cagcagggat ggccaggatg gcgacttctg caccgggtat ttcttccacc gtgtgatatg
     1561 acgtcagctc ctgtatggta tcagcttttg gattgacaat gtatatatct ccggggtaac
     1621 gggtgtcttt cagatttttt atcactttac caccgggttt atgtaagtca ttggaccctc
     1681 ccacgaccac tatgcttttt ggattgagaa attcttttgt aatcattaat atagctggtt
     1741 tgtcttacaa agttagcaat ttgtttggca atttatctaa atttgtagcc tcataaaaca
     1801 gaatcataat gatattgata gcagacagcg ggtcgacaaa agttgcctgg agggtactac
     1861 acaaagacgg caccatacag gccatccaga ccattgggat caatcccttt ttcgtttcgg
     1921 aaccggacat cctgagaacc cttgaagaga acctcaaacc ggccgtacgg ggggatgtga
     1981 gccaggtgtt cttttacggt gccggagtgg caggtaaaga caagatcgat atcctgacac
     2041 gttgtttcca gactgcattc cccggatgtt cggtggaagc caattcggac ctgctggctg
     2101 cagcgcgctg tcttttcggt tcggaaaaag gcattgccgc catcctggga acgggagcca
     2161 atacatgttt ctacgacggg gagaagatcg tggacaacgt gcctgcctgc ggttacatcc
     2221 tgggagatga aggcggaggg gcggtgcttg ggaaaaagct cgtttcggat tacctgaaaa
     2281 ggatgttgcc ggcagacctg agtgaagctt ttgaaaaaca atataaactc ggcctgctgg
     2341 atatcataga gaaagtgtac aggcaaccct tgcctaacag gttcctggct tcgttcgcat
     2401 tattcctggg agatcaccag tcgcatccct atgttcagga gttcctgggg aaagcctttg
     2461 atgaattctt ttcacgcaac atcattcatt atgattacaa gacttatccg gtaaatattg
     2521 tggggtcggt tgcctattat ttcagggatg aagtcatggc atcggctgca cgccacggga
     2581 tgaagatcgg caagattgtc cgcactccta ttgaaggtct ggttgcatac cacaggaatc
     2641 ttatgaccca acaggaatga tacaaaaagt cacagaacaa cggtcgcggt acgaccatct
     2701 ggaaaagatg tccgtcaggg aattgctgga aaatatcaac agggaggacc agaccgtacc
     2761 cctggcggtg atcccctgca tcccgcagat agaaaaattg gtggaagaga tagtcacccg
     2821 catgaaggaa ggaggaagac tgttttatat gggtgcggga acaagcggca ggctcggcat
     2881 tctggatgcc tcggagatcc cgcccacttt cggggctccg tacgacctgg tcatcggcct
     2941 gatagccgga ggcgatgcag ccatccgcag ggcggtggaa aaggccgagg acagtctgga
     3001 ggcaggatgg gaggagctca gggtgcacca tgtgggaacc aacgatgtgg tggtaggaat
     3061 agctgcttcg gggacgactc cttacgtggt gggggccctt cgcgaggcca ggaaaatggg
     3121 cctgctgaca gcatgcataa catgcaatcc cggaactccc gtgacgctgg aagccgaaat
     3181 tcctatagtt gccgttgtgg ggccggagtt tgtcacaggt tccacccgga tgaagtcggg
     3241 cactgcccag aaactcatcc tgaacatgat atccacgacc gtcatgatcc gtttgggaca
     3301 tgtgataggg aacaaaatgg tggatatgca gctgtccaat gccaaactaa ttgacagggg
     3361 cacaaaaatg atcatggaag atacagggat cgaggattac gacaaagcgc gttccctgtt
     3421 gcttcaatac ggtacggtac ggtcagctgt caatgctgtg aatgataaga gccatgtttg
     3481 aacaacgtta cgtttcagac ctgttgcaga aggcggtcga agccttttcc cggcttccgg
     3541 ggattggtcc caaatcggct ttgaggctgg ctctgtacct gctcaggcag gaagaggaag
     3601 agtctgaaaa cctggcccaa agcatcgttg aattgcggag gaaggtagtg tattgctcag
     3661 agtgccggat gatatccgat gctgccttgt gtccggtttg ttccaacccg gagcgggacc
     3721 gaggtatcat ctgtgttgta gaaaacgtgc gtgatgttat gagcattgaa catacgggac
     3781 gctacaaggg attgtaccac gtgctgggag ggatcatatc cccgatcgag ggcatcgggc
     3841 cgacagacct ttccatagaa cccctgatgg acaggctcgc caggggcgtg gtaagggaag
     3901 tgatccttgc cctgagcact accatggaag gagagaccac gtgcttttac ctgtacaaga
     3961 aaatggcatc tcttgacttg aatgtcacca ccattgcccg aggggtgggc tttggcgacc
     4021 aactggaata tactgacgaa ctgaccttgg gcaggtctat tgagaacaga ctgcctttta
     4081 aacctgaata atctttttct gcatatggaa catacatttc cgctatcctg gctgttaacc
     4141 gcatttgcag tgtatacggc cctgcttttc tttgtttcct ggctaacctc acgaaaggcc
     4201 gacagcaaca ctttctttcg cgggaacaag aagtccccgt ggcccattgt agcttacggc
     4261 atggtaggta catccatcag cggagtgacc ttcatctctg ttccgggcaa tgtgatgaat
     4321 gaaaattttt actacatgcc cctggtactt ggctttgtaa taggatacct ggtgatagcc
     4381 aaggttttgt tgccactgta ttataaaatg aacgtgacat ccatctacac atacctggaa
     4441 aaccgtttcg gtttcacgac ctaccggaca ggggccgccg tgttcatggt ttcacgggtg
     4501 ctgggagcgg ctgtgcgtat ctttatcgtg gtactggtgc tgcacacttt cctgcctgcc
     4561 ggttctgttc ccttctggat tgtggcaggc gtgttcatgc tgctcatatt cctctacacc
     4621 tacaggggag gggtaaaaac catcatctgg accgatgtcc tgcagactac cttcatgtta
     4681 ctggctgttg tattgacggt catttccata gccaaagcca tggactggga ttttaggcag
     4741 atggcaggcg ctgtcacttc atccgattat tccgactggt tcaactggga ctggagtgca
     4801 tccaacaacg cagtgaaaca attcatcagc gggatattca taaccatagt catgacgggg
     4861 ctggaccagg agatgatgca gaaaaacctg agttgcaaga acctgcaatc ggcacagaaa
     4921 aatatctaca ccaccagtat cacccttgtg gtggtcaata tcctcttcct taccctgggg
     4981 gccgttatgg ccatttatgt gaaccagcac ctgggtggca tacagggcat agggcttgcc
     5041 gatgccgcag gcagcttcac caaagcacag acagataagt tgtttcctgt gatcgcatcc
     5101 caataccttg gtctgggtgc aggccttttc tttatcatcg gcctgatatc ggcatcgtac
     5161 cccagcgcag gcggggcact tacctccctg accacctctt tttgcattga ttttctcggc
     5221 tttaacaaaa agaaagagat gccacaggaa aggaaggaca agatccgcaa agtcatccac
     5281 gcttcttttg ccatgctatt ctttctcata atccttgtgt tgtatgtcgt gaacaaccag
     5341 gcgataatca acctggtata cctgttggcc gcctatactt acggaccatt gctgggcttt
     5401 ttctttttcg gcatattgac aaaataccgt gtccgggaca gggtaacccc gtatatcgca
     5461 ctggctgcac cggctctatg ttacctggca gacctggcct tgaaaacctg gtttgatttc
     5521 ggcttgggat ttaccgtgtt gatagtgaat ggattgcttg tgttttttgg aatgtgggtt
     5581 ttccgtgaaa aaacactccg cgaaggcgcc tgatgccagt tcaaaaaaaa aggttacatt
     5641 tgtactgatt attcaaacag aatatgccgg acggttagtt accatataag ccattgcttg
     5701 caaggactta aaacgattta ggtatgcgtt gtcgtgaaaa acatacaggg agttcaagtg
     5761 cctgctggca aatagcccat aaagtcagat caaagccgcg aagttgaagg tagacaaacg
     5821 tttacaacag tatgttttac ctgttaaact tcgcattatg attgaaatat tttacaaatc
     5881 caaagggcaa atcttaaacg atacagatgt aaggatcctg gacaaactgg gattggacga
     5941 tgttttatgg attgatttgc acgatcccac aggggatgaa aaaagggctg ccgaggattt
     6001 tttagataca acgctgcaga gcagggccca ggccgaggaa attgaatcct cttcccgtta
     6061 ctcggaaaca gaaaccacca tttttgccaa caccaacttc ctgattcccg gacccgacga
     6121 ttatacgtcc atccccgtgt ctttcatatt gtcagagagc atcctggtca ccctgcggca
     6181 cacgccgctc aggaccctgg ccgaagttca gcgtaagttg ctgaacaact tgaagctcta
     6241 tccaacgggt taccacatca tggtaagcat tctggagaac aggattgatg tggatgccga
     6301 cctggttgag atcctttcaa aggaaatcac gcaactctcc aggagaattg acctgggaga
     6361 atccatggac gagacattcc ttctggatgt aaaccagttg caggaaaaca ccatggtcat
     6421 caggacaaac ggtgttgaca agcaaaggat cctctccggt atttttaaaa gcgataagtt
     6481 cccaagggac ttgtacacaa aactggaaat cctgaacaaa gacatcgact cgctcataca
     6541 ccacgccaat ttcagttttg aacggcttga gtacctgcag aacacagccc tgggtcttat
     6601 caacctggaa cagaacaaac tgatgcgggt attcaccttt gtatcccttt tactcatgcc
     6661 tcccacgctc atagccagca tttacggcat gaacgtgaaa ttgcccatag tgtcgggaag
     6721 ccagttatgg gatttcataa tcttgctcct gttgatgctc ttgtcggtgg tgatagcgat
     6781 ctttttgttc aaaagaagaa agatgatcaa ataagagggg cggttttctc ctgcagccac
     6841 cgtgattctt cataagtcag aaaaggtgcc attttttcat agactctccg gttataccgg
     6901 ttgagccatt cgatttcccc tctttccatc agggacacta ggattgcaga tgtatctatg
     6961 tggcataggg taagggtttc aaagccaagc caggagccga atccgttttc ttccagctcc
     7021 ttgcataaca ggatgttttc atgccttacc ccatgggacc cttcccggta tatgcccggc
     7081 tcgttggaag tgaccatccc ggggagcaga tcctggtctt tccacgtctg ccttatatcc
     7141 tggggacctt cgtgaacaca aaggaaatgc cctatcccgt gcccggttcc gtgtccgtaa
     7201 ttgcggaggt gtctccacag tgggtccttg gcaagcatat ccaggtgtgt cccttttgtc
     7261 cctttcagaa atacggccct gctcagggct atcatacctt tcagggcaat ggtgtaatct
     7321 tccctttcca gaggggtaag cggcccaagc gggacggtcc gggtgatgtc tgtcgtccca
     7381 tacaggtaat gacctcctga atctatgaga aagagacctt ttttctccag ccggctgcca
     7441 ggaccggcca gataattgta gtggggcagt gcggcattgg ccccgtatgc cgggatatat
     7501 tgaaagctgt cacccatgga gtcaggggaa agggagcgca tccaggacaa tttcttagcg
     7561 acatcctttt ctgtcaaaag gacatcctgc tccagggact tttccagcca gaagaagaaa
     7621 cgttccatag ccaggccgtc atacaggaac gcttttctga atccttcggt ttcgaccgga
     7681 ttttttacgg ccttggccag taccacaggc gatggcatat cttttactcc tgtactgcca
     7741 aaacgtgata acagcaggct gaacagagaa taattcagcg tatccccgtc tgccatgaac
     7801 accgattcta atggaaggcc ttccaatact gaagtcacgg cttcataagg cgcaataccc
     7861 accccttgct ctctgagaag cctggtcacg ttgtctccca cctttcccgg ggtaatgaac
     7921 aaggtggcgt tcttactgtc aaccagggca taagagacag caacaggatt gtagggaatg
     7981 tcccggctcc tgatattgaa aagccaggca acctggtcca gccctccgag caggatgtgt
     8041 gaacagccct ggctggacaa ctgctcccgc aaccaggtca gcttttcttc tgtgctacgg
     8101 ccactgtatt gtgtttccag gataaagaca gggctcagcg gaagggcggg cctgtccacc
     8161 cacaacgtat cgggaaagga gggagtgttt acaactttgc cggcattggc ctgcagtgaa
     8221 gc
//
LOCUS       DAOSOF010000087         8136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8805_length_8136_cov_4.432372, whole genome shotgun sequence.
ACCESSION   DAOSOF010000087 DAOSOF010000000
VERSION     DAOSOF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8805_length_8136_cov_4.432372"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLM68_05905"
     CDS             <1..499
                     /locus_tag="PLM68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT54933.1"
                     /translation="RIDFKNTIIIMTSNIGSRELKEFGTGVGFATVSSGAAEAAENRR
                     RHIIEKSLGKMFTPEFLNRVDEQILFNALDKEDIYKIIEIELADLYERIQHAGFRVII
                     SQKAKDFVASQGFDPQYGARPLKRAIQRYLEDPLSEAIIRMENTTGSELHVDLNPEGT
                     NTVVI"
     gene            539..3013
                     /gene="gyrA"
                     /locus_tag="PLM68_05910"
     CDS             539..3013
                     /gene="gyrA"
                     /locus_tag="PLM68_05910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT54934.1"
                     /translation="MEDRIQKINIEDEMKSAYIDYSMSVIVSRALPDVRDGLKPVHRR
                     VLFGMSELGLASNKPTKKSARIVGEVLGKYHPHGDSSVYEAMVRMAQDWSMRYMLVEG
                     QGNFGSVDGDSAAAMRYTEARLRRVAEEVLADMDKDTVDFKNNFDDSLQEPSVLPAKV
                     PLLLLNGASGIAVGMATNMPPHNLGEIVDAVCAYIDSDNISVDELLKYVKGPDFPTGG
                     IIYGTSGIREAYETGRGRIVVRAKTDIEVAPSGRETIVVTEIPYMINKRELIEKIADL
                     VEKKKLDGIAFINDESDRNGMRIVIKLKIGVVANVVLNSLFKYTALQSSFSVNNIALV
                     DGRPRLLNLKQLIKYFVRHRHQVVVRRAHFERDQAAKRAHILEGLLKALDILDEVIAL
                     IRASQTVEEARSGLQREFGFSEEQASAIVEMKLRQLTGLERSKLQAEYSQLMELIRNL
                     DDLLASEQLQMKLIKDEMLDIKARFNDPRRTVIECAAGDFNPEDFYPDEDVVITISHM
                     GYIKRTNLNEYRLQGRGGIGSKGSNTREEDFIEHIYTANMHSTMLFFTQHGKCFWLKV
                     YEIPEGNKISKGRAIQNVINIEPGDSVCAYINVDNLTDENYLNNNYIVLCTERGTVKK
                     TRLKAYSNPRSKGVNAITISENDNLLEACLTNGSCELLLGAREGKCVRFDEKNARPIG
                     RTAAGVRGISIEEYDKVVGMICVNTSVEACPHVLVVSENGYGKRSLIEDYRKVNRGGK
                     GVKTLNITEKTGKLIALKAVTDDSDLMIITRSGITIRLAVADIRLAGRATQGVRLINL
                     REGDAIAAVCCVPKSDPLPKEAVEDL"
     gene            3065..4381
                     /locus_tag="PLM68_05915"
     CDS             3065..4381
                     /locus_tag="PLM68_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT54935.1"
                     /translation="MKKFLTAALMLLMAMQLGAQSKEADILLKNFNKAKEQTLDVKRN
                     TKPATWIKYAQTLTDVYSFPTNNLWIGLSSLESKVVLKDQRALSTEYMTIQGEQYEVV
                     KYQDKDLYYNPEGRLSFWIITNPLLKDVNMLTEAYEAYTKAAELDTKGGSKSEIQEGL
                     TLIANNFINDAMASYTLGNYADASVKFEKSFNAASHPALNVIDTTIVYYVALTALMDR
                     DYNRAVKFFEKCLEMDFESAGDTYANLAEAYKQLGDVEKSKELLSSGFTKYPNSQSVL
                     VALINVYLESNDDPNKVLTFIQRAQENEPNNPSLYYAEGNVLKGLGKFEEAIRLYQKS
                     IDTDPNFFFGYFQMGQAYYDKAVDIQTAASDELDDTKYMKMVEELDLMLEKAIPPFEK
                     SFELIQDEEIRQVVVEYLKNIYFRLRTKSPEYEASYEKYNKLLQGE"
     gene            complement(4501..5576)
                     /gene="buk"
                     /locus_tag="PLM68_05920"
                     /pseudo
     CDS             complement(4501..5576)
                     /gene="buk"
                     /locus_tag="PLM68_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
     gene            complement(5666..6568)
                     /locus_tag="PLM68_05925"
     CDS             complement(5666..6568)
                     /locus_tag="PLM68_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOT54936.1"
                     /translation="MKSFEEIFEQLRSQKKKRLVAAWAVDDHTITAVYKAIEKGIVDG
                     ILVGKESQIHAICHEEGIDPKAFTIVNVEQELPAIARAVEMVRNGQGDILMKGQCSTD
                     KYMRAILNKETGLLPPKAVLSHVSVLTHPSYPKFLIIGDMAVIPAPDLNQKVAIAKYL
                     VNTAHALGIAKPKLAFLAATEQMLPGMPACVDAAILSKMADRNQISGCIADGPLALDV
                     CLSPEAVAIKKLQSPVAGDADCILFPNIESANVMYKSLTQLSPGSKQAAIVAGASAPC
                     VLSSRADSIDTKLNSIALAAVTAK"
     gene            complement(6575..7792)
                     /locus_tag="PLM68_05930"
     CDS             complement(6575..7792)
                     /locus_tag="PLM68_05930"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT54937.1"
                     /translation="MIILVLNCGSSSIKYKVLDMKTEKDYTILAQGIVERIGLEMGIV
                     THKRLIGDKEKYKKDLPVPNHTVGIKIILDLLTDGKWGVLESLTQIQSVGHRVAQGGK
                     YYKESVLINDSVKNNIRKLAELAPLHNPAHLLGIDAVEKLLPHAYQVAVFDTAFHQTM
                     PEHAYMYAIPVELYEKYDIRRYGFHGTSHKFVARKGCELTGLDFNKAKVITCHLGNGS
                     SITAVDGGKSIDTSMGFTPLEGLIMGTRTGDIDPSIVTFLQEKEGWTAAQTNDFLNKK
                     CGLLGLFGESSDCRDIDEAVLKGNKRAILAQNAFAYRVLKYIGAYTAAMNGADLIVFT
                     GGIGENDSMIRHFIGTRLGFLGVSFDQEKNKTVRGQNAILSTNGTKTQVVVISTDEEL
                     VIATDTLNLTNKK"
     gene            7914..>8136
                     /locus_tag="PLM68_05935"
     CDS             7914..>8136
                     /locus_tag="PLM68_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54938.1"
                     /translation="MLPPALAVITGTLGGLFLGERELVRFDRGALVLLYTTLALLLVF
                     LVSLRIFRRRPGHVHKRFGSPYISQSQLRY"
BASE COUNT         2018 a         1958 c         2140 g         2020 t
ORIGIN      
        1 aaggatagat tttaagaaca ccattataat tatgacctcc aacattggca gccgtgaatt
       61 gaaggaattc ggcacagggg ttggttttgc aaccgtttca tccggggctg ccgaagcggc
      121 cgagaaccgc cgccgtcata tcatagaaaa gagcctgggc aagatgttca cccccgagtt
      181 cctgaaccgg gtggatgagc agatcctttt caacgcactg gacaaggaag atatttacaa
      241 gatcatagag attgaactcg ctgacctcta cgaacgcata caacatgcag gcttcagggt
      301 tatcatttct caaaaggcga aggattttgt ggcttcacag ggattcgacc cgcaatatgg
      361 tgcccgtccc ctgaagcgcg ccatacagcg atacctggag gatcctcttt cagaggccat
      421 catccgcatg gagaacacca ccggttccga actgcatgtt gacctgaatc cggaaggtac
      481 caataccgtt gtcatttaac ggaattattt gtatttttga aggattattt tgaaccgtat
      541 ggaggataga attcagaaga taaacatcga ggacgaaatg aagagtgcgt acatagatta
      601 ttctatgtcc gtaatcgttt cccgcgccct tcccgatgtt cgcgacggct tgaaacccgt
      661 tcaccgcagg gtgctttttg gcatgtccga actggggctt gccagcaaca aaccaaccaa
      721 gaaatcggcc cgcatagtag gggaggtgct gggtaagtac cacccgcacg gcgattccag
      781 tgtttacgag gctatggtgc gcatggcaca ggactggagc atgcgttaca tgctggtgga
      841 aggtcagggt aattttggtt cagtagacgg ggattccgcc gctgccatgc gttataccga
      901 ggcacgcctg cgccgcgttg ccgaggaggt tctggccgac atggacaagg acaccgtgga
      961 tttcaagaac aactttgacg attcattgca ggaaccctct gtcttgccgg ccaaggtccc
     1021 cctgttgctg ttgaacggcg ccagcgggat agccgtggga atggccacca acatgccgcc
     1081 ccacaacctt ggcgaaatag tggatgctgt ttgtgcctac atagattccg acaacatcag
     1141 tgtggacgag ttactcaaat acgttaaagg ccccgatttc ccgacaggtg gtatcattta
     1201 cggcacctcg ggcatccgcg aagcctatga aacaggccgc ggccgtattg tggtgcgtgc
     1261 caaaaccgac atagaggtgg cgccgagcgg ccgtgaaacc attgtggtga ccgagatccc
     1321 ctacatgatc aacaagcgcg agctgataga gaagatcgcc gacctggtgg aaaagaagaa
     1381 actggacggg attgccttca taaacgacga gagcgaccgt aacggcatgc gcattgtcat
     1441 caaactcaag attggggtgg tggccaatgt ggtgctcaac agccttttca aatatacagc
     1501 cctgcagagt tcattctctg taaacaacat agcgttggtg gacggtcgtc ccaggctcct
     1561 gaacctgaaa cagctgatca agtatttcgt gcgtcaccgc caccaggtgg tggtccgccg
     1621 tgcccatttt gaacgtgacc aggctgccaa acgcgcccac atcctggaag ggctgctcaa
     1681 ggccctggat atcctggacg aagtgatcgc cctgatacgc gcttcgcaga ctgtggaaga
     1741 ggcacgctca gggttgcagc gggaattcgg attcagcgaa gagcaggcct cggccattgt
     1801 ggaaatgaag cttcgccagc ttaccgggct ggaacgctcc aagctgcagg ccgagtatag
     1861 ccagctgatg gaacttatcc gcaacctgga tgatcttctg gcaagcgagc agctgcagat
     1921 gaagctcatc aaagacgaga tgctggacat caaggcccgc ttcaatgatc cccgcaggac
     1981 agtcattgag tgcgctgcag gggattttaa ccctgaagat ttctatcccg acgaggatgt
     2041 ggtcatcacc atttcacaca tgggatatat aaaacggacc aacctgaatg aataccggct
     2101 gcagggcagg ggagggattg gttcaaaggg aagcaatacc cgggaagagg atttcattga
     2161 acacatctat acagccaaca tgcacagcac catgctgttc tttacccagc acgggaagtg
     2221 tttctggctc aaggtatacg aaatcccgga aggcaacaag atatccaagg gacgcgccat
     2281 tcagaatgtt atcaacattg agccgggtga cagcgtttgt gcctacataa acgtggataa
     2341 cctgacagat gaaaactatc tgaacaacaa ttacattgta ttgtgcacag aacgtggaac
     2401 ggtcaagaag accaggttga aagcttattc caatccacgt tccaaagggg tgaatgccat
     2461 taccataagc gagaacgaca acctcctgga agcctgcctg accaatggct cctgcgaatt
     2521 gttgctggga gcgcgtgaag gaaagtgtgt ccgctttgat gaaaagaatg cgcgccccat
     2581 aggccgtacg gctgccggcg tacgcggaat atccatagaa gagtacgata aggtagttgg
     2641 catgatatgt gtgaatacat ctgtagaagc ctgtcctcat gtgttggtag tgagtgagaa
     2701 cggatacggc aaacgttcct tgatagaaga ttacagaaag gtcaaccggg gtggcaaggg
     2761 agtgaaaacc ctgaacatta cggaaaaaac cggcaagctc attgccctca aggcggtgac
     2821 tgacgacagt gaccttatga tcatcacccg ctcgggtatt accatccgtt tggcggtggc
     2881 cgatatccgc ctggcgggcc gggccacaca aggagtacgg ttgatcaacc tgcgtgaagg
     2941 agatgccata gctgccgtct gctgcgtacc caaaagcgat cccctgccta aggaagcagt
     3001 agaggatttg taatatttga ataatttgaa ataatttttt aacagcacaa acacataata
     3061 catcatgaaa aaatttctga cagctgccct gatgttgttg atggcaatgc aattgggcgc
     3121 acaatctaaa gaggcggaca tcctccttaa aaacttcaac aaggcaaaag agcaaaccct
     3181 ggatgtaaag cgcaacacca aacccgcaac ctggataaag tatgcacaaa cactgaccga
     3241 cgtgtattct tttccgacca acaacctgtg gataggtctc tcctcgctgg agtcaaaggt
     3301 cgtgctgaag gaccagcgtg ccctttcgac agagtacatg accatacagg gagaacagta
     3361 tgaagtggta aaataccagg acaaagacct ctattacaat cccgaaggca gactgtcctt
     3421 ctggatcatt accaatccgc tgctcaagga tgtgaacatg ctgaccgagg catacgaagc
     3481 ctataccaag gctgccgaac tggacacaaa gggtggcagc aaatctgaaa tccaggaagg
     3541 attgaccttg attgccaaca acttcatcaa cgatgccatg gcatcctaca cgctgggcaa
     3601 ttatgcagat gcttctgtga aattcgaaaa atcatttaac gcggcatcgc atccggcttt
     3661 gaatgtaatt gatacgacca ttgtgtatta tgtggctttg acggctttga tggatcggga
     3721 ttacaaccgt gccgtgaaat tcttcgaaaa gtgcctggaa atggactttg aatctgccgg
     3781 cgacacgtat gcaaacctgg cagaagcgta taaacaactg ggtgatgttg agaaaagcaa
     3841 agagcttctt tccagcgggt ttacaaaata tcccaacagc cagagcgttc ttgtcgcttt
     3901 gattaacgta tacctggaga gcaatgatga ccctaacaag gtgcttacct ttatccagcg
     3961 tgcacaggaa aacgagccca acaatcccag tctgtactat gcagaaggca acgtgctaaa
     4021 aggtttgggc aaatttgaag aagctatccg cctgtaccaa aaatccattg atacagatcc
     4081 aaatttcttc ttcggatatt tccagatggg ccaggcttac tatgacaagg ctgttgatat
     4141 ccaaacagct gcttctgacg agctggacga caccaagtat atgaaaatgg tggaagagct
     4201 ggacctgatg cttgaaaaag ccattcctcc ctttgaaaag agttttgaac tgatccagga
     4261 tgaggaaatc cgccaggtgg tggttgaata cctgaagaac atctacttcc gcctgcgtac
     4321 caagagtccc gagtacgaag catcctacga gaaatacaat aagctgctgc agggcgagtg
     4381 agtgccgcgc actcttgatt cattaggctg gctatcagca ctataacaaa agagctccct
     4441 gggggagctc ttttgttaat tatatggtat acagatcaat aaatcaagag tgcgaggcag
     4501 tcaccccgca tacacctggg caatctcttc tcctgttagc acgttgtagg cattattggc
     4561 cagggcaacc atttcgtctt ctccggcgta tgtgaagcag ggcgccagga atcccacctg
     4621 ttcagtaatc aggtctatga agcgtttgtt gtgggcaatg cctccggtaa agaggattgc
     4681 gtcaatttta ccgcacagga caacagccgt tgcgcctatc tgttttgata tggtataggc
     4741 catggcctgg tacagtcggg tagcttttgg atcaccggcc tgcaccttgt cacatagttc
     4801 cataaaggag ttggtgccca ggtgggccac catgcctcct tttccggtaa gcatcctttt
     4861 gacctcctga agggtgtatt ttcccgagaa gcaaagctct gccaattgca aggcaggaac
     4921 gctgccggca cgttcgggtg cgatgggacc gtctccgttc agggtgttgt tcacgtcaat
     4981 aacccggcct ttgcgatgag cgcctaccga tatgcctccg cccaggtgca ccactatcag
     5041 gttaagatct tcataggatt ttcccatgat gctggcatga cgcttggcga tggccttctg
     5101 gttcagggcg tggaagatcg aaaccctttc aatttgggga actccggtaa cacgagccac
     5161 atcctgcagc tcatccacca ccaccggatc ggatatgaag gcctggcatc ctggaatttc
     5221 cgaagctatg ctgtgggcca gcaaacctcc caggttggag gcatgctctc cgccaatacc
     5281 ctcggccagg tgattgagca tcagctcgtt cacgcgataa gtccctgatg gaatcggatg
     5341 gaccaggcca ccccggccga taacagcagc gaaatggtcg gtttggatat tattgttctt
     5401 caactcattg aagatgttcg ctttccggaa ctcatactgc tggcaaatcc tgggataagg
     5461 agccaggtac tgggggtatg gtatatggtt tttccaaaca cctctttcct gccctcaaaa
     5521 acggcaattt ttgtcgatgt agagccggga ttgataacca ggatttgttc tttcataact
     5581 ttatctttat tgtgaacatc tgcaatatgt cgtaaccagt gccgttttac attaaccgtg
     5641 gccggagcat tggagcagct tgcttttact tagcagttac tgcagccagg gcgatggagt
     5701 tgagcttggt atctatggaa tcggcgcggc ttgaaaggac gcagggagcc gaggcgccgg
     5761 ccacaatggc ggcttgtttt gaccctgggc tcagctgggt gaggcttttg tacatcacgt
     5821 tggcgctttc aatgttgggg aagaggatgc agtccgcatc acctgccaca gggctctgca
     5881 gtttcttgat ggcgacagcc tcggggctaa ggcaaacatc cagggccagg gggccgtcag
     5941 ctatgcagcc ggagatctgg ttgcggtcgg ccatttttga gagaattgca gcatccacgc
     6001 aggcgggcat gccgggaagc atctgttcgg tagccgccag gaaggccagt ttgggtttgg
     6061 caatgcccag ggcgtgggcg gtgttgacca ggtactttgc aatggctact ttctggttca
     6121 ggtccggtgc tgggataact gccatgtccc caatgatcag gaatttgggg tagctggggt
     6181 gtgtaagtac cgaaacgtgg ctcagtacgg cctttggagg cagcaggccg gtttccttgt
     6241 tcaggatggc acgcatgtat ttgtctgtgc tgcactggcc tttcatgagg atgtcgccct
     6301 gtccgttgcg taccatttcc acggcacggg ctatggccgg gagttcctgt tcaacattga
     6361 ctatggtgaa ggctttgggg tcaatgcctt cttcatgaca gatggcatgg atctgtgatt
     6421 ccttgcctac caggataccg tccactatgc ctttttctat ggctttataa acagcggtta
     6481 ttgtgtggtc gtccactgcc caggcggcaa ccagtctttt ctttttctgt gagcgcagct
     6541 gctcgaatat ttcttcaaag gatttcatgg gtgtttattt tttatttgtc agattcaggg
     6601 tatctgttgc gatgaccagt tcctcgtctg tgctgatgac aacaacctgg gtttttgtcc
     6661 cgttagtgga aagtatggcg ttttgcccac gaacagtttt gtttttttcc tggtcaaagg
     6721 aaacacccag gaagccgagt cgtgtgccta tgaaatggcg gatcatactg tcattctcgc
     6781 cgattccgcc agtgaataca atcagatccg ctccgttcat ggcagccgta tatgctccga
     6841 tgtattttag caccctgtag gcaaaggcat tctgtgccag gatcgcccgt ttgttaccct
     6901 ttagtaccgc ttcgtctata tctctgcaat ccgagctttc tccaaacagg ccgaggaggc
     6961 cgcatttctt gttcaggaaa tcatttgtct gggccgcagt ccagccctct ttttcctgca
     7021 ggaaggtgac gatggagggg tcgatatcgc cagttcgggt gcccatgatc aagccttcca
     7081 gaggggtgaa acccatggag gtgtctattg atttgccccc gtcaacggcg gttatggaag
     7141 aaccgttacc caggtgacag gtgatgactt ttgccttgtt gaagtccagt ccggtgagtt
     7201 cacaaccttt gcgggccaca aatttatgac ttgttccgtg gaacccgtag cggcggatat
     7261 cgtatttttc atatagctct acaggtatgg cgtacatgta ggcatgttcc ggcatggtct
     7321 ggtggaaggc ggtatcgaaa acagccacct ggtaggcatg gggcagcagt ttttcaacag
     7381 catctatacc cagcaggtgg gctgggttgt gcagcggggc cagctcggcc agcttcctga
     7441 tgttattctt gacactatcg ttaatcaata cactttcctt gtaatattta cctccctgtg
     7501 ccacgcggtg tcctacggac tgaatctggg taagtgattc aagtacaccc catttgccat
     7561 ctgtgagcag gtccaggatt atttttatac ctactgtatg gttgggaaca ggtagatctt
     7621 tcttgtattt ctctttgtct cctatgagcc tcttatgcgt tacaatcccc atttccagcc
     7681 ctatgcgttc cactatgccc tgtgccagaa tggtataatc cttttctgtc ttcatgtcaa
     7741 gaactttgta cttgatggaa gaacttccgc agtttaaaac aagaatgatc atgattggtt
     7801 ttggtaaggt tttttattat atttgaacta tctgtaatag attgtaatag aatttacaaa
     7861 attacggaaa ctatatgtga cttgcaaggg tgaggaggag gaaaaatagt gtgatgttgc
     7921 cgcccgcatt ggccgtcatt accggcacac tgggcggctt atttttgggg gaaagagagc
     7981 ttgtcaggtt tgacagaggg gcgctggtcc ttttgtatac aacacttgcg ctgctgttgg
     8041 ttttcctggt ctcattacgt attttcagaa gacggccggg gcatgtacac aaacggtttg
     8101 gttccccata tatttcccag agccagctac ggtacc
//
LOCUS       DAOSOF010000088         8093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8866_length_8093_cov_4.613710, whole genome shotgun sequence.
ACCESSION   DAOSOF010000088 DAOSOF010000000
VERSION     DAOSOF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_8093_cov_4.613710"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1692
                     /locus_tag="PLM68_05940"
     CDS             55..1692
                     /locus_tag="PLM68_05940"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOT54939.1"
                     /translation="MDKYVIGLDYGTDSIRAVVVNARTGETVATSVKYYPRWAEGKYC
                     LPSVNRYRQHPLDYIEVLEASVKEALSLAPAGTASRVAGIAFDTTGSTPVFTDETGTP
                     LAFLPGFEDNPNAMFVLWKDHTAIAEAAEINKLCSSWSIDYSAYVGGIYSSEWFWAKA
                     LHILREDRAVREKAYSIVEHCEWMPALLTGVKKSSDIVRSRCAQGHKAMWHPRWGGLP
                     GEDFLVALDPLLAGFRSRLFEDTHTADKPVGTLTPEWAGRLGLGTGVVVAGGAFDCHM
                     GAVGAGITPNTFVRVIGTSTCDIMVAPYEEIGSSLIRGICGQVDGSVIPGMIGLEAGQ
                     SGFGDVYAWFRQVLEFPLRQLLPEGEQVNELSEKIIPALTAEAEKIPVGESTLIATDW
                     MNGRRTPDANQLLKGAIAGITLGTSAPHIFRALVEATAYGSRAIVERFRNEGVRIDNV
                     IGIGGIALKSPFVMQTLSDVLNMPIDVCRTDQACALGAAMFAATAAGIYTKVEEAQQA
                     MTSGFAMTYHPRKEMAEAYDKAYQRYLELGRYCEDSI"
     gene            complement(1798..3174)
                     /locus_tag="PLM68_05945"
     CDS             complement(1798..3174)
                     /locus_tag="PLM68_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT54940.1"
                     /translation="MKIKVRVSVFFLIVVLFAPADCFGQFFGDRNINRDSLMRVRDSL
                     HSITVADHADMMARLGIKEVRPGRDPNSPDPARQPNYDELMANPYCFYPDALTAFSGQ
                     QVKNARMWNRVRRPELVKVFEDEVYGRIPDNVHAVAWHVTSEEKASLGGTPVVIRHLT
                     GIVDNAACPEISVEILAQVIWPDNGRKNMPVILELSWMMTDPLHPFGQGIPWQQQVVE
                     RGWAAAQIVPGSIQADGGYGLREGIIGLCNQGQYRKPTDWGTLRAWGWGVSVLIDYFQ
                     GDDLFDAAKVAVEGVSRYGKAALVAMAFDQRIAAGFICSSGKGGAAPWRRYCGESLEN
                     LTSDGEYHWMAGNFIKYGAGSLTADDLPVDQHELIALCAPRPCFISAGSFQTDKWVDI
                     AGMFMAALKASPVYELLGRKGLGTDVLPVAGFGLLDADLAYRQHHGGHEAGPNWPFFL
                     DFFARYID"
     gene            complement(3171..3674)
                     /locus_tag="PLM68_05950"
     CDS             complement(3171..3674)
                     /locus_tag="PLM68_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT54941.1"
                     /translation="MTTGAVSPMVDGYVLHESFDDAALNNNLANVPYMIGFTLNDMGN
                     MAPGIADFCLNREQAGDKAYAYQFARPLPTDGRENVLKGAFHSSDLWYVFKSFKNSWR
                     PWTEGDWDLSEVMLTAWTNFARFGDPNGQQGGQWAPYTSENPRFMIFRLDDNDAVNSE
                     MGEPLRP"
     gene            complement(3755..4618)
                     /locus_tag="PLM68_05955"
     CDS             complement(3755..4618)
                     /locus_tag="PLM68_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT54942.1"
                     /translation="MKKLLLLFFTFSLFGCAGSNPVLTIEGGKVQGVKADVRGVYVYR
                     GIPYAAPPIRELRWKAPQPVIPWEGIKIADTFGHPGFQATHYPGGYTTEWGYGEESAY
                     SEDCLYLNVWTKAPGQTDRKLPVALWIFGGGLREGWGSEPEFDGQEWAGKDVVLVSFN
                     YRLGVFGFLTHPGLSAESPDHVSGNYGILDQIEALKWVKENIAQFGGDPDNVTIFGQS
                     AGGRSVKTLSASPLARGLFSKAIIMSASGLATASPSSSMSAYMSAFAPLTLEESEQQT
                     KEVMDWRALPT"
     gene            complement(4615..5964)
                     /locus_tag="PLM68_05960"
     CDS             complement(4615..5964)
                     /locus_tag="PLM68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT54943.1"
                     /translation="MFRKITLSVTLLLLGGAAMAQNPIIRDQFTADPSALVVGDRIYV
                     FPSHDIPAPDDFPRKDWFCMGDYHVFSSENLTDWTDHGVILDQKDVPWGNPKGYSMWA
                     PDCAEKNGKYYFFFPDFMKSDGKPGRFSPFRAGVAVADRPEGPYTPRETPIEGVFGID
                     PCIFVDDDGQGYLVWPARGINIAKLNDDWSQLAEEPTPVGNLPSPGLIEGPYLFKANG
                     KYYMTFPWARKDTEVLAYSMADNIYGPYEFKGVFFEEWPNKCWTNHHSIVNFKDQWYI
                     FYHHNAYSPDFDKNRSACADSLFFEADGSIRMVKPTLRGIGITRAESDIQIDRYSAIS
                     EAGAAIAYLDTTYYFAGWKTVFTSPDAWVQYNTVQFDGDCRGAVLRVNAPKGGMLLLQ
                     SGNKTIAKVKVPVTEGWKEIPVRVRGIEKGIHHLTVSSLSPNVTHVDWLRFSKKAKP"
     gene            complement(5975..6901)
                     /locus_tag="PLM68_05965"
     CDS             complement(5975..6901)
                     /locus_tag="PLM68_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT54944.1"
                     /translation="MKNTILTFVFSLCTIVVTAQGFQMPEGPGEGTYSRSWKDVNYAS
                     DDQECHNLDIYLPKEQKNIYQAVIVIYGSAWFSNNAKTMASASIGTPLLQAGFAVVSI
                     NHRSSIQAIWPAQIQDVKAAIRYVRANAASYDIDPSSIGITGFSSGGHLATFAGVTND
                     VKTLASGDVTVDIEGSVGNYVSTSSRVQAVVDWFGPVEMARMSNCEAPNDESSPEAML
                     IGKKDPRREPDWIKLISPINFVDKDDPEILIIHGDADNVVPHCQSVYLKSAYDNAGGK
                     ATFISVPGGGHGPVCFDTEYFKKMTDFFNKLL"
     gene            complement(6920..8038)
                     /locus_tag="PLM68_05970"
     CDS             complement(6920..8038)
                     /locus_tag="PLM68_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT54945.1"
                     /translation="MKKIVTLISAVILMVSLGYAQESTTEVKEDFKPSTLNQPGKEYP
                     MVNSQGYARFLIKAPDAQAVSVSLGLGGRGGTVLQKTEDGFWTGTTAGPLDEGFHYYH
                     LTVDGGVFNDPGTLNFYGSTRWESGIEIPAHDQDFYALKNVPHGKVQQVLFYSPSTET
                     VRRAFVYTPPCYGKEPGKKYPVLYLQHGWGEDETAWSNQGHANLIMDNLIAAGKIDPF
                     IIVMTYGMTNEIKFGSGLRNFDIKPFQTVLVDELIPYIDAHFSTLADKDHRAMAGLSM
                     GGMETKMITLNRPETFSYYALFSGGVYAPEDIKDKNQVKLIFLSCGSKERPEGIIKST
                     SDLKNAGFNAVGYVSENTAHEFLSWRRSLYQLAPLLFK"
BASE COUNT         1963 a         2145 c         1994 g         1991 t
ORIGIN      
        1 aagagttgaa actgaacgag atctattacc tgttgaacaa agcgttgtgt tagcatggat
       61 aaatacgtta ttgggctcga ttacggaacc gattcgatcc gtgctgtggt tgtaaacgcc
      121 cgtaccggtg aaactgtcgc cacctcggta aaatattatc cccgttgggc ggaaggcaag
      181 tactgtttgc cctctgtcaa caggtaccgc caacaccccc tggattatat tgaagtgctt
      241 gaagcctcgg taaaggaagc cctttcgctg gctcctgcag gcactgcctc acgggtagcc
      301 ggcatagctt tcgatacaac agggagcaca cctgtcttca ctgatgaaac aggcacgccg
      361 ctggcctttc tgccaggctt tgaagataat cccaacgcca tgttcgtgct ctggaaagac
      421 cacacggcca tcgccgaggc tgcggagatc aacaagctgt gcagctcctg gagcattgat
      481 tacagtgctt acgtgggagg catctattca tctgaatggt tctgggccaa ggcattgcat
      541 atcctccggg aagacagggc agtaagggaa aaagcctact ccattgtaga acattgcgaa
      601 tggatgcctg cattgctgac cggggtgaaa aaatcttcag acattgtcag aagccgctgc
      661 gcacagggcc acaaggccat gtggcacccc cgctggggag gcttgcccgg agaggatttc
      721 cttgtcgcgc ttgaccccct gctggctggt ttcaggagca ggcttttcga agacacccat
      781 acggccgaca agccggtggg tacgctcacc ccggagtggg ctggacgcct gggccttggt
      841 acaggtgtag tggtcgcagg aggcgccttt gactgccata tgggagccgt gggagcagga
      901 ataacgccaa acacttttgt cagggtcatc gggacatcta cctgtgatat catggtagct
      961 ccctatgagg aaatagggtc ttctctcatt cgtggcattt gcggacaggt tgacggttca
     1021 gtgatccccg ggatgattgg tctcgaggcc ggtcaatcgg gtttcggaga tgtttatgcc
     1081 tggttcagac aagtgcttga gtttcccctc aggcaactgt tgccggaagg agaacaggtc
     1141 aatgagcttt ccgaaaagat cattccggcg ctaacggccg aagctgagaa aattcctgtg
     1201 ggagaaagca ctctcatagc taccgactgg atgaacggcc gtcgcacccc cgatgccaat
     1261 cagttgctca aaggggctat tgcgggaatt accctgggta cttcggctcc gcatatcttc
     1321 agggctttgg tcgaggctac cgcttatggt tccagggcca ttgtggaacg tttccggaac
     1381 gagggcgtcc ggattgataa tgtgatcggg atcggaggca ttgcactgaa gtcgcccttc
     1441 gtgatgcaaa ccctgagcga tgtgctgaat atgcccattg acgtttgccg taccgaccag
     1501 gcctgtgcgc tgggggccgc catgttcgct gccacggctg caggtatcta cacaaaggtg
     1561 gaggaagcac aacaggccat gacctcgggc tttgccatga cataccatcc ccgcaaagaa
     1621 atggcagaag catatgacaa ggcataccag agatatcttg aactgggacg ttactgcgaa
     1681 gattccatct aataaaataa ctgggtgaat ggatccgcga aggacctccc atttggtgaa
     1741 ccaaacgggt ccctcccttc gcgggacaag cctttgcttg acccattcac ccagttatca
     1801 gtcaatataa cgggcaaaga aatccaaaaa gaacggccaa ttgggaccgg cttcgtgacc
     1861 gccgtgatgc tgcctgtatg ccaggtcagc gtccagcaat ccaaagcccg ccaccggaag
     1921 aacgtctgta cccagtcctt tcctgcccaa aagctcataa acaggcgaag cttttaaagc
     1981 agccatgaac atgccggcaa tatctaccca cttgtcggtc tggaagctcc ccgccgaaat
     2041 gaaacaagga cgcggtgcac ataaggcgat cagttcatgc tgatccacgg gaaggtcatc
     2101 agcggtaagc gacccggccc catatttgat gaaattcccg gccatccaat ggtattcacc
     2161 gtcagaggta aggttttcga ggctctcgcc acagtaccgg cgccacgggg ctgccccgcc
     2221 ctttcccgaa gaacagatga aaccggctgc aatcctctgg tcaaaagcca tggcaacaag
     2281 agcagccttt ccatatcttg acaccccttc aaccgccact ttggcggcat caaaaaggtc
     2341 atcgccctgg aaataatcaa tcaatacgga tactccccat ccccacgccc ggagcgttcc
     2401 ccagtcagtt ggcttacggt attgtccctg gttgcataaa ccaatgatac cttcccttag
     2461 accgtatcct ccgtcagcct ggatggatcc gggaacaatt tgggcggcag cccagccgcg
     2521 ctcaaccact tgctgttgcc aggggattcc ctgcccgaag ggatggaggg gatctgtcat
     2581 catccaggat aactccagga tgaccggcat gtttttccgg ccgttatccg gccaaatgac
     2641 ctgggcaagg atctccacag atatttcggg gcaggcagca ttatccacga tcccggtcaa
     2701 atgacgtatc actaccgggg tgcctcccag gcttgctttc tcttcgctgg ttacatgcca
     2761 tgcaacggca tgaacattgt ccggaatccg tccgtaaacc tcatcttcaa ataccttgac
     2821 aagttcaggt ctcctgaccc tgttccacat cctggcattc ttcacctgtt gtccgctgaa
     2881 tgcagtcagt gcgtccgggt agaaacaata cggatttgcc atcagctcat cgtagtttgg
     2941 ctgcctggcg ggatctggag aattgggatc acgtccggga cgtacctctt ttatacccag
     3001 ccgggccatc atgtctgcat gatcggccac cgtgattgaa tgcaggctgt ctcttaccct
     3061 catcagactg tcccggttta tattcctgtc accgaagaat tgcccgaaac agtctgcggg
     3121 ggcgaacaat acaacaatta aaaaaaagac ggatacacgt acttttatct tcatggacgc
     3181 aagggttctc ccatttccga atttaccgca tcgttatcat ccagcctgaa gatcatgaac
     3241 cggggatttt cactggtgta tggggcccat tgacctcctt gctgcccgtt gggatccccg
     3301 aacctggcaa agttcgtcca ggcggtaagc atcacttcag aaaggtccca atcaccttcc
     3361 gtccagggtc tccagctgtt tttgaaagac ttgaacacat accagagatc ggaggagtgg
     3421 aatgcccctt tcagcacgtt ttccctgcca tcggtcggca gagggcgcgc aaactgataa
     3481 gcatacgcct tatcccctgc ctgctcgcgg ttaaggcaga aatcagctat tccgggagcc
     3541 atgttgccca tgtcgttcag ggtgaatccg atcatataag gcacattcgc cagattgttg
     3601 ttgagagcgg catcgtcaaa actctcatgc aagacatatc cgtctaccat gggtgagacc
     3661 gctcccgttg tcatccatgt acgctttccg gtaactgatt gatagatact tcccaggcta
     3721 aaaataaatt cggtggaagc ggcccgcatc ttgtctaggt cggtaaggcc cgccaatcca
     3781 ttacttcctt tgtctgctgc tctgactctt ccagcgttaa cggcgcaaat gcactcatat
     3841 aggctgacat ggaagaggat ggagaagcag tggccagacc actggcactc atgatgatgg
     3901 ctttgctgaa caaacccctg gccaggggag atgcactaag ggtttttaca ctgcgacctc
     3961 cggcactttg tccgaaaatg gtaacattat cgggatcgcc tccaaactgc gctatgtttt
     4021 ccttcaccca tttgagggct tctatctggt caagaatacc atagttgcct gaaacatgat
     4081 caggactctc tgctgaaagt ccggggtgtg tcaggaagcc gaaaacaccc agacggtaat
     4141 tgaaagaaac cagcacaacg tccttgcctg cccactcttg tccgtcaaac tcgggctcgg
     4201 acccccatcc ttccctcagg ccgcctccaa agatccacag ggcgacggga agcttcctgt
     4261 cagtttgtcc gggagctttg gtccatacat tcaggtaaag gcaatcctcg ctgtaggctg
     4321 actcttcacc atagccccac tccgtggtat aacctccggg ataatgtgtt gcctggaaac
     4381 ctgggtgccc gaaggtatcg gcgatcttta ttccttccca gggaatcacc ggctgcggag
     4441 ctttccaccg gagctccctg atgggagggg ctgcataagg aataccccgg taaacataaa
     4501 ctcccctgac atcggctttg actccctgaa ccttgccgcc ttcaatggta agtaccggat
     4561 tcgagccggc gcatccgaac agcgagaagg taaaaaacag tagcaacaat tttttcatgg
     4621 ctttgccttt tttgaaaacc tgagccagtc aacatgcgtc acattgggac tcaaagaact
     4681 gacagtcaaa tgatgaatgc ctttctcaat cccccggacg cgcaccggga tctctttcca
     4741 cccttctgtc acgggcacct tgacttttgc aatcgtctta ttcccgctct gtaaaagcag
     4801 cattcctccc ttgggagcat tgacccggag gacggccccg cggcagtcac catcaaattg
     4861 aacggtattg tattgtaccc atgcgtcagg actggtgaaa acagtcttcc atccggcaaa
     4921 ataataggtg gtatccaggt atgctatggc agcacctgct tcactgattg ctgaataacg
     4981 gtctatctgg atatcagatt ctgcccgggt gattcctatg cctcgcaggg tcggcttaac
     5041 catgcggatg cttccgtcag cttcaaagaa aagactgtcg gcacatgcgg agcggttctt
     5101 gtcgaaatca ggggaatagg cgttatggtg gtagaagatg taccactgat ccttgaaatt
     5161 gacgatggaa tggtggtttg tccagcattt gttcggccat tcttcaaaaa agacaccctt
     5221 gaactcatag gggccgtaga tattatctgc catggaatat gccaggactt ccgtatcctt
     5281 acgtgcccag ggaaaggtca tatagtattt tccgttcgcc ttgaacaggt acgggccttc
     5341 aataagacct ggagaaggca ggttcccgac aggtgtaggc tcttcggcca gctgggacca
     5401 gtcatcgttg agctttgcga tattgatccc tctggcgggc cataccaggt atccctgacc
     5461 gtcatcatcc acaaaaatgc acgggtcaat tccaaagacc ccttcgattg gcgtttcccg
     5521 gggagtataa ggaccttctg gccggtcagc cacagctacc cctgcacgga aagggctaaa
     5581 acgtccgggc ttgccgtcac tcttcatgaa gtcagggaag aaaaaataat atttcccgtt
     5641 cttctctgca cagtccggag cccacataga atagcctttc ggattccccc acgggacatc
     5701 tttctggtcc aggatcaccc cgtggtctgt ccagtcggtc aggttctctg atgagaaaac
     5761 gtgataatct cccatacaga accagtcctt gcggggaaaa tcatcaggtg cgggaatgtc
     5821 gtgagacggg aaaacataga ttctgtcccc taccacaagt gcagaagggt cagccgtgaa
     5881 ctgatccctt atgatagggt tctgggccat ggctgcccct ccaagcaaca atagagttac
     5941 tgataatgta atttttctaa acattagtaa ttcattaaag taatttattg aagaagtctg
     6001 tcatcttctt aaaatattct gtatcaaaac atacgggtcc atgtccgcca ccgggcacag
     6061 atatgaaagt agctttacct ccggcattgt cataagcgct tttcaggtaa acgctttggc
     6121 aatgaggcac cacattatct gcatccccat gaatgataag gatttccgga tcatctttgt
     6181 ccacaaagtt gatggggctg attagtttga tccaatcagg ttcccggcga ggatccttct
     6241 ttcctataag catggcttcc ggtgaagatt catcattggg agcctcgcag tttgacatac
     6301 gggccatctc cacgggacca aaccagtcaa ccactgcctg gacccggctg cttgttgata
     6361 cataattgcc cacagaacct tcaatatcta ctgtaacatc acctgatgca agtgtcttaa
     6421 catcgtttgt tacaccggca aaagtggcaa ggtgtccgcc ggatgagaat ccggtgatcc
     6481 caatagacga aggatctatg tcatagctgg cagcatttgc gcgaacgtag cggatggctg
     6541 ctttcacgtc ctgaatttgt gccggccaga tcgcctggat gctggaacgg tgattaatag
     6601 agacaaccgc aaaacccgcc tgcagaagag gagtacctat ggatgcagat gccattgtct
     6661 tggcattgtt ggaaaaccat gcgctgccgt aaatcacaat taccgcctga tagatgtttt
     6721 tttgttcttt aggcaggtat atgtccagat tgtggcattc ctggtcatct gaggcgtaat
     6781 ttacgtcctt ccaactcctg gaataagtac cttcacctgg tccttccggc atctggaatc
     6841 cctgtgccgt tacgacaatg gtacacaggg aaaatacgaa tgtcaatatt gtatttttca
     6901 tatcggaatt gggtaacgac tatttaaaaa gtaacggagc aagctggtac aaacttcttc
     6961 gccagctcag gaactcatgt gccgtgtttt ccgatacata cccgacggca ttgaatcctg
     7021 cattcttaag gtcagacgtt gattttatga ttccctcggg gcgttctttg ctcccgcaac
     7081 ttaaaaagat gagttttacc tggtttttgt ctttaatgtc ctccggtgca taaacacctc
     7141 cgctgaacaa ggcatagtaa gaaaaggttt cgggacggtt cagggtgatc attttggttt
     7201 ccatgccacc cattgaaaga ccggccatgg cacggtgatc cttatcggca agagtactga
     7261 aatgtgcgtc aatgtaaggg atcaactcat ccaccaatac cgtctggaac ggtttgatat
     7321 cgaaattcct gagcccgctg ccgaacttga tctcattggt catcccgtaa gtcatcacta
     7381 tgatgaaagg atctatcttg cctgctgcaa tcaggttgtc catgatcagg tttgcgtgtc
     7441 cctggtttga ccaggcggtc tcatcctcac cccagccatg ttgaaggtat aagaccggat
     7501 atttcttgcc gggttcttta ccgtagcatg gaggtgtata tacaaaagcg cgccgtacag
     7561 tttcagtgct aggtgaatag aaaagcacct gctgcacctt gccgtgggga acgttcttca
     7621 gggcataaaa atcctgatca tgggcgggta tctctatccc gctttcccaa cgtgtagaac
     7681 catagaaatt caacgttccc ggatcgttga acacaccccc gtccactgtc agatgatagt
     7741 agtggaatcc ttcatccagc ggaccggcag tagttccagt ccaaaatccg tcctctgtct
     7801 tttgcagtac cgtacctcca cgccccccca agcccaggct cacactaaca gcctgtgcgt
     7861 caggagcttt aataagaaac cgggcatatc cctgggaatt gaccattgga tactcttttc
     7921 cgggttggtt aagggttgaa ggtttaaagt cttcttttac ttctgtggtg ctctcctggg
     7981 catagcccaa acttaccatt aatataacgg cacttattag agtaacaatc tttttcataa
     8041 aaattaatct ttaaataaca gtggtgcaaa ttgatacagg cttcttctcc agc
//
LOCUS       DAOSOF010000089         8011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_8988_length_8011_cov_4.618904, whole genome shotgun sequence.
ACCESSION   DAOSOF010000089 DAOSOF010000000
VERSION     DAOSOF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8988_length_8011_cov_4.618904"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLM68_05975"
     CDS             complement(<1..340)
                     /locus_tag="PLM68_05975"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT54946.1"
                     /translation="MNKIFNILEKSLPFVFGFCVLLFFGLVYPHHMHYHEQFQLFLTT
                     PDFFLEIAAKPGGISDYLGMFLTQFFLFSWAGAAITALLIMSLQWLIHTIAHKIRPAR
                     AWFALSFIPPI"
     gene            complement(333..1808)
                     /locus_tag="PLM68_05980"
     CDS             complement(333..1808)
                     /locus_tag="PLM68_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54947.1"
                     /translation="MKKLLDIRILLALFILGACRGPGEPVKEQVPPPIYPDYTGVTIP
                     VNIAPLNFMVDGATRLRVNLSVGDMNLYLAGPGRIRIPLRRWHSLLASAASLGEDITV
                     TVLVKMQGQWHQYLPFPWHVTPDTPDPYLSYRLIEPGYEVWNTIQLAERHIGTFRERI
                     IADNQLTENSCMNCHTYGNQDQGLSFFHLRGPGGGTILNRNGELRKIDLFAPGMLSGV
                     VYGNFHPSGRYAVYSTNLVLPGFHTQKGERLEVYDSDSDLVIVDLEQNQVIPFPEGFP
                     PEFRTFPVFSATGDAVYYCNAPKMTVPDSIYHLRYDLLKISFDAETGTWGNKVDTVVR
                     ASAMGLSVCHPKTSPDGRYILYSMAHYGTFPIWHQETDLWLLDLDTGTTDRLEEVNSP
                     YSDTYHSWSSNSRWFVFASKRDDGLYGKPYYCYVDFQGNAHKPFVLPQRNPQKYHNTL
                     KSYNIPEISRGKLPFGASDIERLYFKVPAEKVSIKQSVDHE"
     gene            complement(1809..3389)
                     /locus_tag="PLM68_05985"
     CDS             complement(1809..3389)
                     /locus_tag="PLM68_05985"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT54948.1"
                     /translation="MRKGTSITTLIAGIIAAGCFLFFWLLYPYHNLLKEQMTLFLHSA
                     EHFLSYLTKPAWLSSAAGDLLTQFYIYPAGGALLVTVSLLAEWFLFRRVFRKSGMRSL
                     YSSLSALVPVIIEFLLHCGLHYPLSSTFSTLIALVTLIAFMRLRSNSLYFWLAFPAAA
                     MMYLLAGHGIILFIAGWMIIGILGRRRRWYSMALPALCSLALPLLLRATFLLSPVQAY
                     RYPVREPLTLSVDMTWKKILELDYESQQQNWPRVLVLSRDYALQNRIASYYTNLALSQ
                     TGLMGEWLFHFYQPGPAGLFLPATQYSTPLMITFSNEAFFYLGDLNMAQHAAMLGMIF
                     SPYHRSARLVKRLAEIHIAIGDGPAAEKYLGLLDKTLFYRKWARQARALTAGEDMPLR
                     KKIPSIDTVRLADDYLTSLEILLQSNPENKTALDYLLCYHLLNKDIPSFRDAYNTWGY
                     SAGERMPGVYAQALIVSLYQEGADNETLKEYNIPVPVISEFMDYTRAYEQAKGLSAPL
                     QERFGNTFWFYYHFAMLE"
     gene            complement(3411..3980)
                     /locus_tag="PLM68_05990"
     CDS             complement(3411..3980)
                     /locus_tag="PLM68_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOT54949.1"
                     /translation="MKRILSLSLVCMMILSCSENKNYVIEGTFENEKYDGEYVFLLPL
                     DGVMPRIIDSVQVKDRSFVFTGKADSAQMKIIRMRHLLRLDIQELLVVVEPGNIWVRL
                     DTVSAAGGTPQNEKLQAWKEVKMQSDETMNLLKRMSQTDVDQETAGRISEQWEKIQAD
                     FKKYSLQFIEENRGTAVGRFVSDMTGGSQ"
     gene            complement(4044..4880)
                     /locus_tag="PLM68_05995"
     CDS             complement(4044..4880)
                     /locus_tag="PLM68_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54950.1"
                     /translation="MKKSLILAIFAVFVLLSACQPVEIRQDLKGGITESELNISAVPQ
                     IRDGKNSNYIDLNSDGNACLSSWDFGVGLFVGTKGTVKVMMKGPNDIIFTGLNADGTK
                     ITKTLTVQVDALYDVEPEWALFCGATGEKTWVWDNVTGPGVWGNGGFKGCAAPCWWVV
                     SLGDINGQAPGEGAGASMTFTIAGARLIKNYNDGTSVQGSFTFDMSKIILDDGGNVWA
                     KGKLNTKNVTVLCGKSPNEGGAPVNSYDILKLTENNMVLSYPEPGVGSWGTAWFWMFK
                     KQ"
     gene            complement(4909..6681)
                     /locus_tag="PLM68_06000"
     CDS             complement(4909..6681)
                     /locus_tag="PLM68_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT54951.1"
                     /translation="MKKILFIALAALLFSSCEAFLDTESYTKKNTGNYPKTADDAIQM
                     VTGVYATLNRVLANCGNSYYFAAQLLSDDMFAGGGENDKHWTSLEHLMYVDLNQYNSF
                     WSDRYSGISRANMAIANLDKVEDEKLRNQLMGETLFLRALFYFELVQMFGDVPLITQV
                     PQSVSEVAEYPAQSTQEEIYAFIAADLKKASDIMPNDKWNATVSGEGHATRWAAQALL
                     ARVFLFYTGFYGKTELPMMDEEFNITGKVTKAEVIAGLEDCINNSGHTLANDFRRLWP
                     YSNSLSAKDYYYVEDLKNKGEYWLKDGTNPETVFAIKCSHLASWSTTTGYSNQYLLFF
                     AIRNAGTGDQYKNVFPFGQGWGAGPVNPKLWEDWATQEPTDKRRKGSILAAADRPNYV
                     YGADALMEETTLWQKKVIAITGAKEYEADGSIKTLYNSFTSSTEYYGDGESDDFQLGH
                     ETDVILIRFADVLLMHSELTETATGMNQVRARVGLPAVSYSFPALQKERRFELAFEGL
                     RWGDMRRWGDNYAISALTGQLGHTIWNRGVQTIMKDQGAGYSARYQATRGFLPIPSSE
                     VDLSDGVLKQNPGWGPEAVFGSWN"
     gene            complement(6694..>8011)
                     /locus_tag="PLM68_06005"
     CDS             complement(6694..>8011)
                     /locus_tag="PLM68_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT54952.1"
                     /translation="RADGSSNFARGKRWGYFPSASVGWTVSNEDFMAGAADIIDFLKI
                     RASWGQNGNCSIGNFQYLSTIAFDVSNGYYFAADKKTQTVGGYADILANPDVTWETSE
                     QLNIGLDARFARGRLGLAFDWYQKMTRDWLLTAPIPGVYGLNAPAVNGGDVKNSGVEL
                     SLTWDDFVGDFRYGISLNGSYNKNEVTRIANTEGIIHGAANVLSQGTTEMYRAQVGYP
                     LGYFFGYKTAGVFQNWDQVNNTAAKYDGAKPGDLIFVDTNKDGKITEDDRTMIGSGHP
                     DFLMGFNLYFAWKGLDFSITGAGAFGQEIAKSYRSFADSPLQNFTTDIFGRWTGEGTS
                     NKLPRLTTGSNLNWQNISDIYIEKGDYVKIQNVQIGYDFKQAFRKLPFGQVRLYVSAQ
                     NLYTFTKYSGLDPEIGYGYDQGWVQGIDLGYYPAPRTYMVGLNLKF"
BASE COUNT         1950 a         1957 c         2074 g         2030 t
ORIGIN      
        1 agattggagg aatgaaggag agggcaaacc atgcccgggc agggcggatt ttatgggcaa
       61 ttgtatgtat aagccattgc agggacatga taagcagggc tgttatagcc gctccggccc
      121 aggaaaacag gaagaattgc gtcaggaaca tacccaggta atccgatatg cctcctggtt
      181 ttgccgctat ctccagaaaa aagtcaggcg tggtcagaaa tagctggaat tgttcatggt
      241 agtgcatatg gtgcgggtat accaggccaa aaaacagtaa aacgcaaaat ccaaaaacaa
      301 agggcagcga cttctcaagg atattaaaaa ttttattcat gatctacgct ttgtttgata
      361 ctgacctttt ctgccggcac tttgaaatag aggcgctcta tgtctgatgc tccaaagggc
      421 agttttcccc gggatatttc ggggatatta tacgatttga gggtgttgtg atatttttgc
      481 ggattcctct gagggagcac aaaaggtttg tgtgcatttc cctgaaaatc cacataacag
      541 taatacggtt tgccgtatag tccgtcatcc cgtttactgg caaagacaaa ccagcggctg
      601 ttggaagacc agctgtggta ggtatcggag taaggagagt tgacttcttc cagtctgtca
      661 gttgttccgg tatccaggtc cagcagccag agatctgttt cctggtgcca gatgggaaag
      721 gtcccgtaat gggccattga atagagtata taccggccgt cgggggatgt tttgggatgg
      781 cagacagaca accccatggc cgaggccctt accactgtat cgactttgtt tccccaggtc
      841 cctgtttcag catcaaagga gatcttcagc aaatcatacc gcaggtggta aatactgtcg
      901 ggaactgtca tttttggcgc attgcaatag taaacggcgt cccctgtagc cgagaatact
      961 ggaaatgtcc ggaattcagg gggaaagcct tcaggaaacg gtatcacctg gttctgttca
     1021 agatccacta tcaccaggtc ggaatcgctg tcataaacct ccagccgttc tcccttctgc
     1081 gtatggaaac cgggcaagac caggttggtg gaatacacgg cataacgtcc cgatgggtga
     1141 aaattgccgt aaaccacacc cgacagcatt cccggtgcaa acaggtcaat tttcctgagt
     1201 tcaccgttcc tgttgaggat tgtaccacca ccggggccac gcaggtggaa aaacgacaga
     1261 ccttgatcct ggttgccata agtgtggcaa ttcatgcaac tgttctctgt aagctggttg
     1321 tctgctatga tcctttcacg aaaagtcccg atatgtcttt ctgccagttg tatggtattc
     1381 caaacttcgt atcccggctc gatcaggcgg tagctcaggt agggatcggg ggtgtccggg
     1441 gtaacatgcc acgggaaggg gaggtattgg tgccactgtc cttgcatttt aaccagtacg
     1501 gtaaccgtta tgtcttctcc cagggaagca gcactggcca gcaggctgtg ccacctgcgc
     1561 agaggtattc gaatccttcc gggtcccgcc aggtacaggt tcatatcacc aaccgacagg
     1621 ttgaccctca gccgggtggc tccgtcaacc atgaagttta ggggggctat gttcacaggg
     1681 atggtaactc ccgtgtaatc cggatagatg ggtggcggga cctgttcttt gacaggttcc
     1741 ccaggaccac ggcacgcgcc aaggatgaac agtgccaaca ggattcttat gtcaagcagc
     1801 ttcttcatct attcaagcat cgcaaaatga taataaaacc agaatgtgtt cccgaaacgt
     1861 tcctgcaggg gggcagacag gcctttggct tgttcatatg cccgggtgta atccatgaac
     1921 tcactgatca caggtacagg aatgttgtat tcttttagtg tttcattgtc ggctccttcc
     1981 tgatatagag atacaataag cgcctgggcg tacactccag gcattctttc accggcagag
     2041 taaccccacg tgttgtacgc atcgcgaaag gagggaatat ccttgttcaa gagatggtaa
     2101 catagcaggt aatccagggc cgtcttgttc tccggattgc tctgcaggag tatttccagg
     2161 gaggtcagat aatcatccgc cagccgcacg gtatctatgg atgggatttt tttgcgtagt
     2221 ggcatatctt ccccggcggt caatgcacgt gcctgccggg cccatttcct gtaaaacagg
     2281 gttttatcca gaagccccag gtatttttct gctgccggtc cgtcgccaat ggcgatatgt
     2341 atctcggcca gtcttttcac aagacgcgcg ctcctgtggt atggtgagaa gatcattccc
     2401 agcatggcgg catgttgtgc catgttcagg tctcccaggt aaaaaaacgc ctcgttgctg
     2461 aaggttatca tcaacggggt ggaatactgc gttgcaggca ggaaaagtcc tgcaggaccg
     2521 ggctggtaaa aatgaaacag ccattctccc atcagaccgg tctgagatag ggccaggttg
     2581 gtgtaatatg aagcaatcct gttctgcagg gcgtaatccc gggaaaggac caataccctg
     2641 ggccagttct gttgctggct ttcataatcc agttcaagga tctttttcca ggtcatgtcc
     2701 acactaaggg taaggggttc cctgacaggg tacctgtatg cctgaactgg ggataagaga
     2761 aacgttgcac ggagaagcaa gggaagtgcc agtgagcaga gagcaggcag agccatggag
     2821 taccagcgcc tgcgtctgcc aagtatcccg attatcatcc agccagctat aaacaggatg
     2881 atcccgtgtc cggccagcag gtacatcatg gcggctgcgg gaaaagccag ccaaaaataa
     2941 agggaattgg agcgcaaccg cataaaggcg atcagagtga ccagggcaat cagggtagag
     3001 aaagtgctgc tcaaaggata atgcagtccg caatggagca gaaactctat gattacaggt
     3061 actaatgctg acagagagga ataaagggaa cgcatgccgg atttccggaa aacccgcctg
     3121 aataaaaacc actctgccag taatgataca gtcaccagta aagcaccacc tgccgggtat
     3181 atgtaaaact gcgtgagcag gtctcctgca gctgatgaca gccaggccgg tttggtaagg
     3241 taagatagaa aatgttcagc agaatgaagg aagagggtca tttgttcctt cagcaggttg
     3301 tggtaaggat agagcagcca aaagaacagg aagcatcctg cagcaatgat gccggcgatc
     3361 agtgtggtta tgcttgttcc tttgcgcatg atgaatacca gcggatggtg ttactgcgag
     3421 ccaccggtca tgtcgctgac aaagcggccc acggcagttc cccggttctc ttcaatgaat
     3481 tgaaggctgt attttttaaa gtcggcctga attttttccc actgttccga gatacgcccg
     3541 gcggtttcct gatccacgtc tgtctggctc attctcttga gcaggttcat ggtctcatcg
     3601 gattgcattt tcacttcttt ccaggcttgc aatttctcat tttgcggagt acctcccgct
     3661 gcacttaccg tatccagcct gacccatatg ttcccgggtt caaccaccac caggagttcc
     3721 tggatatcca gccgtaacaa gtgtcgcata cgtatgatct tcatctgtgc cgagtcggct
     3781 ttaccggtaa agacgaatga ccggtcctta acctgcacgg agtctatgat acggggcatt
     3841 acaccgtcca aaggcaagag aaacacgtat tcaccatcgt atttttcatt ctcaaaggtg
     3901 ccctcgatca catagttctt gttttccgag cacgataaga tcatcatgca tacaagggat
     3961 aatgataaga ttcttttcat gcttgcaaat atagcaaaaa agggtggctc gtgcgagtca
     4021 cccttttttc atgctcagat agtttactgc tttttgaaca tccagaacca ggcagtgccc
     4081 catgagccga cacccggctc gggataggac aataccatgt tgttttctgt aagtttcagg
     4141 atgtcatagc tgttaacagg tgctccgcct tcgttggggg attttccaca aagcacggtg
     4201 acattctttg tgttcaattt tcccttggcc catacgttgc caccgtcatc caggatgatc
     4261 ttggacatgt caaatgtgaa agatccctga acagaagtac catcgttgta gttcttgatc
     4321 agacgcgcac ccgcaatggt aaatgtcatg ctggcaccgg caccttcacc gggagcctgt
     4381 ccgttgatgt caccaagaga gacaacccac cagcagggag cagcacaacc tttgaaaccg
     4441 ccgttacccc agacaccggg tccggtaaca ttgtcccata cccaggtctt ttccccggtt
     4501 gcaccgcaga aaagagccca ttcaggttcc acgtcataca aggcatcaac ctgcacggtc
     4561 agtgttttgg taatctttgt gccatctgcg ttcagtccgg tgaaaataat gtcgttgggt
     4621 cctttcatca ttaccttgac agtacccttg gttcccacga acaagccaac gccgaaatcc
     4681 caggaagaga ggcaggcgtt gccgtcgctg ttcaggtcaa tataattgga gttcttaccg
     4741 tccctgattt gcggaaccgc agagatattg agctcgcttt ccgttattcc tcccttgaga
     4801 tcctgtctga tctcgacggg ctgacaagcg gaaagaagta caaaaacggc aaaaatcgca
     4861 aggattaaac tttttttcat atcgtgagtt ttttaatcgt ttaaaatact agttccagct
     4921 tccaaaaacg gcttccggac cccagccagg attctgtttc aatacaccat cggaaagatc
     4981 cacttcggaa gaaggaatgg gcaggaatcc gcgggttgcc tggtaacgtg cggaatagcc
     5041 agctccctgg tctttcatta tagtttgtac gccacggttc cagatcgtat gaccaagttg
     5101 tccggtaagg gcgctgatgg catagttgtc accccagcgg cgcatatctc cccagcgaag
     5161 accttcaaag gcaagttcaa aacggcgctc cttttgcagg gccgggaatg aatagcttac
     5221 agcaggcaga cccactcggg cacgtacctg gttcatacca gtggctgttt cggtcagctc
     5281 ggaatgcatg agcaatacat cggcaaaacg gataaggatg acatcggtct cgtgtcccag
     5341 ctggaagtcg tctgattctc cgtcaccgta gtattcggta gaagaggtaa agctgttgta
     5401 gagcgttttg atgcttccgt ctgcttcgta ttccttggca ccagtgatgg caatcacttt
     5461 cttttgccac agtgtggttt cttccatcag ggcgtctgcg ccatagacat agttgggtct
     5521 gtcagcagca gccagaatgg agcccttgcg gcgcttgtcg gtaggttcct gggttgccca
     5581 gtcttcccaa agttttggat tgaccggccc tgcgccccaa ccttgtccga aaggaaacac
     5641 gttcttgtac tggtcaccgg ttccagcatt acggatggca aagaacagca ggtactggtt
     5701 tgaatatccg gttgtcgtac tccagcttgc caggtgtgaa cacttgatgg caaaaactgt
     5761 ttccgggttg gtcccgtcct tgagccagta ctcacccttg ttcttgagat cttctacata
     5821 gtagtagtct ttggcactca gggaattgct gtagggccac aacctgcgga aatcgttggc
     5881 cagcgtgtga ccgctgttat tgatacaatc ttccaggccg gcgataacct cggcttttgt
     5941 aaccttaccg gttatattga attcctcgtc catcatggga agttcagtct tgccgtagaa
     6001 tccggtgtag aacaggaaga cacgggccag cagggcctgt gcagcccagc gtgtggcatg
     6061 gccttccccg gaaacggtgg cattccattt gtcgttaggc atgatatcgg aagctttctt
     6121 gaggtcggct gcaatgaagg cgtagatctc ttcctgtgta gactgtgcag gatattctgc
     6181 aacttccgat acactctggg gaacctgggt gatcagggga acatccccga acatctgcac
     6241 cagttcaaaa tagaacaggg cacgcaggaa cagggtttct cccatcagct ggttacggag
     6301 tttttcgtct tccactttat ccaggtttgc aatggccatg ttggcgcggc tgatacctga
     6361 atacctgtca gaccagaaac tattgtattg gttcaggtca acatacatca ggtgctccag
     6421 actggtccag tgcttgtcgt tctctccgcc acctgcgaac atgtcgtcag acaggagctg
     6481 ggctgcaaaa tagtaggagt ttccacagtt tgcaagcacc ctgttgaggg tggcgtacac
     6541 cccggtgacc atctggatgg catcgtctgc cgtcttagga tagttaccgg tgtttttctt
     6601 ggtgtagctc tctgtgtcaa ggaaagcttc acaactcgaa aacagcaggg cagctagtgc
     6661 tatgaataat atctttttca tactgtaaat caattaaaat ttaagattta aacctaccat
     6721 atacgtacgt ggtgctgggt agtaaccaag gtcgattccc tgtacccagc cctgatcata
     6781 tccgtatccg atctcgggat caagtccgga atatttggtg aaggtataaa ggttctgtgc
     6841 agaaacatac aaacggacct gtccgaaagg caatttgcgg aaagcttgtt tgaaatcgta
     6901 tccgatctgt acattctgga tctttacgta gtctcctttt tcaatataaa tgtcagagat
     6961 attctgccag ttcaggttac tgcctgtagt caggcgtggc agcttgttgg aagtgccttc
     7021 accggtccag cgtccgaaaa tgtcggttgt gaagttttgc aggggagagt cagcaaatga
     7081 acggtatgat tttgcgatct cctgtccaaa ggcaccagca ccggttatac tgaagtccag
     7141 tcctttccag gcaaaataaa gattgaatcc catcaggaag tcaggatgtc cgctaccgat
     7201 catggtgcgg tcatcttcgg tgatcttgcc gtctttgttg gtgtcaacaa agatcaggtc
     7261 tccgggtttg gctccgtcgt atttggcagc cgtgttgttt acctgatccc agttctggaa
     7321 gacgccggca gttttgtatc cgaagaaata tcccagggga tagccgacct gggcacggta
     7381 catttccgtg gtaccctggc tcagcacgtt ggcagccccg tgtatgatgc cttctgtatt
     7441 ggcaatacgg gtaacttcgt tcttgttgta agaaccgttt agtgaaatgc catagcggaa
     7501 gtcacctaca aaatcgtccc atgtgaggga tagttccaca ccggagttct ttacgtctcc
     7561 accgttaaca gcaggggcgt taaggccgta aacgcctgga atcggggcgg taagcagcca
     7621 gtcgcgggtc atcttctgat accagtcaaa tgccagtcca agacggcccc tggcaaagcg
     7681 tgcatccaga ccgatgttca gctgttcgga agtttcccat gtaacatcgg ggttagccag
     7741 aatgtctgca taacctccca cggtctgggt cttcttgtcg gcagcaaaat aataaccgtt
     7801 gcttacgtca aaggctatgg tggacaggta ctggaagttg ccaatgctgc agttcccgtt
     7861 ttgtccccag ctggcgcgta tcttaaggaa gtcgatgata tcagcggcac cggccatgaa
     7921 atcttcgtta gaaacggtcc agccgacaga agcggaaggg aagtaacccc agcgtttgcc
     7981 gcgggcaaag tttgaagaac cgtcagcgcg g
//
LOCUS       DAOSOF010000090         7970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9052_length_7970_cov_3.533923, whole genome shotgun sequence.
ACCESSION   DAOSOF010000090 DAOSOF010000000
VERSION     DAOSOF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9052_length_7970_cov_3.533923"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1385)
                     /locus_tag="PLM68_06010"
     CDS             complement(93..1385)
                     /locus_tag="PLM68_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225159.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT54953.1"
                     /translation="MAKRIKMKLIYPRWEKLPGQTTFNLPPHGPVVFAAALPERVDVS
                     FTDENVEPVDFEEECDLVAISMMLSTQVKRGWAIAAEFRKRGKKIIFGGISTMLHAEE
                     TVEHGDVVFLGEAEGRMEELIGDWENGRLRKVYNYMGQLPPIESVGPARRDLYKRELY
                     NHKGVQMVDLFHASRGCRFNCYPCAVAYLGGRVFRPRPFDKVIEEMAGIDNNRLFIVD
                     NSLAQNKEWEMQLFKEIIPLKKKWVSHTIEDDPKILDLAAQAGAWYVYQAVFDTSDFI
                     RERIRRYHDYGIGVEGTILLGMDNQSEDDMRRLIDFLLEIDLDLAEFTILTPYPHTKA
                     YEDLMRKGRILDYDWDHYNAGQVVYQPAQVTPKKLQELYEYAWSSFYSQESQELKMTK
                     LFTRVMKREMEDGTWRPRDRKLMNQSFGKEVKRNIGNI"
     gene            complement(1401..2867)
                     /locus_tag="PLM68_06015"
     CDS             complement(1401..2867)
                     /locus_tag="PLM68_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT54954.1"
                     /translation="MSNKYNVVIIGSGLGGLVCGYILSKNGLRVAIVEKNSLPGGCLQ
                     TFVRKGVKFETGMHYIGSMDEGQPLHRYFKYLDLAGNIKLKRLEKTGYDIVSYMGDRY
                     AYANGHEAFTDTLSRHFPGEKNSIKMYIQRMEAIAERSPLYSFRNMEMPYWLDSSNMQ
                     IGVAEYIHSLTGDERLQNVLAGIMPLYGGVAEKTPLYYHALITDFYNKSAFRFVGGSD
                     MVVQSLCDSIRGFGGEIMTGMPVTKVEFNNSGATGVTLLNNEFIEADHVISDIHPAIL
                     MDMIDTPLIRKAYKLRIRSIRQTLSNFTVYIKFKKNTVPYLNYNFYHYPKNSVWDGHF
                     FYMHMCDEPDQKTVRSAVLFESMAWDEVARWKDLPPGNRGAEYEDFKSRKAQQMLLNL
                     EKQFPGTISHIEDFWTSTPLTYSDYTGTLEGSMYGILRDKNEHTKTFISHRTKVPNLY
                     LAGQNINAHGIMGVIIGSVLTCGEIIDINNLTKQIKNA"
     gene            complement(2860..>4847)
                     /locus_tag="PLM68_06020"
     CDS             complement(2860..>4847)
                     /locus_tag="PLM68_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT54955.1"
                     /translation="EGTTLDEALANYEAVRPQIDSYRHSGIGAFLPSRSMRQERIARW
                     NDFWSTRKDPFLVQFNALARKNGFREGSFARFEEILERSYPTGELKDFGPILNNLAVN
                     YISEKPERSLVYTMLHVDALDAPAIEAELNTPESPAFAFDTGSVTRLMISSLSSDFDY
                     VLWICGFIVFLFLTLSFGRLELSVIAFLPLAISWIWILGIMSLLGLEFNIVNIILATF
                     IFGLGDDYTIFITEGMMHEYAYGKKMLSTYKNTVALSALIMLAGIGSLIVAKHPAMRS
                     LAQVTIVGMISVVITSYTVPAYLFRKLVTKRGRYRKTPLTLWNTWWTFFSFTFFLFGV
                     AFLSLSGAVLLLLGGHSERNKLRFHRILQGMCRYVIFHIPGSRMELINCEEDRFSSPS
                     VIVANHQSHLDLMAIIMLHPKIIVMTNNREWNSPFYRTILRFADYLPIEYLDTNHETI
                     HERISQGYSLMIFPEGTRSADCSILRFHKGAFQLAEELKLDITPVLLHGFGHVLPKEN
                     LLLRRGKMTLKVLPRIKAGDLSFGATYQERAKVVRQLFIREYNALCSMVEDAAYFAPA
                     VLHNYLYKGRDVYASVRRSMQKNSNFEAQIKALPASGPCYLEDRNLGEFALTASLVRR
                     DLKIKAYIADPKKRELAAHCISVPDNLTYTQTPETHE"
     assembly_gap    4848..4857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4858..6336)
                     /locus_tag="PLM68_06025"
     CDS             complement(<4858..6336)
                     /locus_tag="PLM68_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT54956.1"
                     /translation="MVQTFLKIYDHFRKNTRSLYVLLALGVILAAGGLFLLDFDEDVS
                     GFLATGKEHERIGYAYRNIGGSDKIIISIAMADQEKETDSGLLMDAADHLAGLLEKDP
                     ARQHISAVTYMSDPALTLEVSDFVTGNMPYFLEERDYARIDSLLQPDVLEQQIVRVRQ
                     TLLSPAGMMLRNTLLSDPLLFSGPLLQRLEGFKPSEHFTVISDYIFSPDGTELILTID
                     SQYPAGDTGNNKKLIGAIGDAIEETLQVFDHEVTISPFGASYIAVTNADRLRKDTWIS
                     VAIALVVIGVLLAFYFRNARQILLIGATILAGSLFAFSMAGVLTSSLSLIAIGAGSVI
                     VGIAVNYPLHFLSHIREGYTPRQTLADIVPPLTTGNITTVGAFLSLLFLSSPAMRGFG
                     LFASLLLMGTIIFVLIFLPHMVRATGPVSNTLKFGMLADSSPERNRWVMLALLIVTLG
                     LWAGNKGVVFNADMQSINYMTPVQRESMEKLLDRTQGSQHVLY"
     gene            complement(6318..7061)
                     /locus_tag="PLM68_06030"
     CDS             complement(6318..7061)
                     /locus_tag="PLM68_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT54957.1"
                     /translation="MAERDEVCVIIPTYNNASSILRVIEQTMEYCSHVIVVCDGSTDN
                     TLQLLDTYAGKIEIISYATNRGKGYALSRAFALAGAKGYRHAITLDADGQHYPDEIPR
                     FVKVMEEHPGALIVGSRSLEQENMPRENTFANRFSNFWFTLQTFERLPDTQTGYRLYP
                     LYRMQKMRFFTNRYETELEILVRLAWKGIPVIPLPIKVFYAPAGERVTHFRKGRDFLR
                     ISLLNTVLTMTALAYGYPSILLHKWFKRS"
     gene            complement(7034..7411)
                     /locus_tag="PLM68_06035"
     CDS             complement(7034..7411)
                     /locus_tag="PLM68_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54958.1"
                     /translation="MFINNLYKIIDKVYDGEEARVTIELDSDSVIYKAHFPGNPITPG
                     ACIVEMGREIASLILEKGLLLEKADNIKFLMGIHPVETPRVTFMLQMTEQGQGLWKVR
                     TEVKNGDNVMTKMTLWLKETKYV"
     gene            complement(7398..>7970)
                     /locus_tag="PLM68_06040"
     CDS             complement(7398..>7970)
                     /locus_tag="PLM68_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT54959.1"
                     /translation="VIPQTVENRHHMMVALKSIHTLECEFQQEREVTILAEKGISRGK
                     LAYEKNKALLWQYQVPDYSGFLLRGKEMTILDPSGKPLRDAGSAGLFRHIGTIILMGI
                     EGSILEDRELFVPEYIPSEDYIHIRLIPSQREMKRLFTDLELFFRKEDYVIQTVVINE
                     TSGDKTTVRMGNIRINRPLDDTTFDAYVYQ"
BASE COUNT         1937 a         1957 c         1960 g         2106 t
ORIGIN      
        1 gcaggatcct caatccacat tttgaaggaa cgtcccattt gcccttgaaa tcaaatattt
       61 catcaaaatg tttatcttga acttccatga tatcagatat ttccaatatt gcgtttgact
      121 tctttcccga acgactgatt catcagtttc ctgtcacggg gcctccaggt gccgtcttcc
      181 atctcgcgtt tcatgacccg tgtaaagagc tttgtcattt ttagttcctg tgactcttga
      241 ctgtagaaag aactccaggc gtactcgtac aactcctgca attttttagg ggtaacctgc
      301 gcaggttggt agaccacctg cccggcattg taatgatccc agtcgtagtc gaggatcctt
      361 cccttgcgca tcaggtcttc ataggccttt gtatgagggt agggcgttag aatggtgaac
      421 tcggcaaggt ccagatcaat ctccagcaaa aagtcaataa gacgacgcat gtcatcttcc
      481 gactggttgt ccattcctaa aagaatcgtt ccttccactc ctatgccgta atcgtggtat
      541 cgcctgatgc gttccctgat gaagtcggaa gtgtcgaaaa cggcctgata aacgtaccat
      601 gcacccgcct gtgcggcaag gtccagtatc tttggatcat cttcaatagt atggcttacc
      661 catttcttct tgagcggaat gatctcctta aacaactgca tttcccattc tttattctgt
      721 gccagggaat tgtcgactat gaacagtcgg ttgttatcaa taccggccat ttcttcaata
      781 accttatcaa agggacgggg cctgaaaaca cggcccccca ggtaagcaac ggcacaggga
      841 taacaattga agcggcatcc gcgcgaggcg tggaacaggt caaccatttg cacccccttg
      901 tggttgtaca gttctctttt gtacaggtct cttcttgccg gtcctactga ttctatggga
      961 ggcagctgtc ccatgtaatt ataaaccttc ctgaggcggc cattttccca gtctccgatc
     1021 agctcttcca tacgcccctc tgcttctccc aaaaacacga catcgccgtg ctcaacggtc
     1081 tcttctgcat gaagcatggt ggaaatgccc ccgaaaatga tctttttccc tcttttcctg
     1141 aactcggcag ctattgccca tccgcgctta acctgcgtgg acaacatcat tgatatggcc
     1201 accagatcgc attcttcttc aaaatctaca ggttctacat tttcgtccgt aaaagaaaca
     1261 tctactcttt ccggcagtgc cgcggcaaat accaccgggc cgtgtggggg aaggttgaag
     1321 gtggtctgtc caggaagctt ctcccagcgc gggtagatga gtttcatttt aattctcttt
     1381 gccatactga tggtgtgttg ttaagcgttc ttgatttgtt tggttagatt gtttatgtca
     1441 atgatttctc cgcaagtaag aacagatccg atgataacac ccatgatgcc gtgggcattg
     1501 atgttctgtc cggccaggta tagattggga acttttgtgc ggtgcgaaat aaatgttttt
     1561 gtgtgttcat tcttatccct gaggattccg tacatggagc cttccagggt tccggtatag
     1621 tcgctgtatg tcaaaggtgt ggaagtccag aaatcttcaa tatgactgat agtgccggga
     1681 aattgcttct ccaggttcaa cagcatttgc tgggcctttc ttgacttgaa gtcctcgtac
     1741 tcggcccccc ggttgcccgg cggcaggtct ttccaccgcg ccacctcatc ccaggccata
     1801 ctttcgaaca gaaccgcgct gcggacagtt ttctggtccg gttcatcgca catgtgcatg
     1861 tagaaaaaat gaccgtccca gacggaattt ttcgggtaat ggtaaaaatt gtaattaagg
     1921 tagggtaccg tattcttttt gaatttgata tatacggtaa aattggacaa ggtctgcctg
     1981 atgctgcgta tcctcagttt gtatgccttt cggatgagcg gcgtatctat catatccatc
     2041 aagatcgccg gatggatatc ggatatgaca tggtccgctt ctatgaattc attgttcagc
     2101 agggtgaccc ccgttgctcc cgagttgttg aattcgacct tcgtgacagg cattcctgtc
     2161 atgatctcgc ctccgaaacc acgaatggaa tcgcataatg attgcacaac catgtcgctt
     2221 ccacccacaa aacggaaagc gctcttgttg tagaaatcgg ttatcagggc atggtaatac
     2281 aagggtgttt tttctgcaac accgccgtat aaaggcatga tgcccgccag aacattttga
     2341 agtctctcat ccccggtaag agaatggatg tattcagcca cgccaatctg catgttgcta
     2401 ctgtccagcc agtaaggcat ttccatgttg cggaaggagt acaatgggga acgttcagcg
     2461 atggcctcca tccgttgtat gtacatcttt atggaattct tctccccggg aaagtgcctt
     2521 gacaatgtat cggtaaaggc ctcgtgtcca ttcgcataag cataacggtc acccatatag
     2581 gacactatgt cgtagccggt tttttccagc cttttcagct tgatattgcc ggcaaggtca
     2641 aggtacttga aataacggtg tagcggttgt ccttcgtcca tgctgcctat gtaatgcatc
     2701 cccgtctcga atttgactcc tttgcgtaca aaagtctgca gacatccccc gggcaggctg
     2761 tttttttcca ctatagctac cctaaggccg ttctttgaaa ggatgtaacc gcaaacaaga
     2821 cctccaaggc cacttcctat gatcaccaca ttgtatttgt tactcatggg tttcaggtgt
     2881 ttgtgtatac gttaaattat ccggaaccga tatgcaatgc gcagccagtt ctcttttttt
     2941 cgggtccgca atatatgctt ttatcttcag atccctgcgt accagcgaag ccgtcagcgc
     3001 aaactcgccc agattccggt cctccaggta acatgggccg cttgcaggta aagccttgat
     3061 ctgagcttca aaattactgt ttttttgcat tgaacgacgc accgaggcat aaacatccct
     3121 tcctttgtaa aggtagttgt gcagaacagc aggagcaaaa tatgcggcat cctcgaccat
     3181 cgagcaaagg gcattatatt ccctgataaa gagctgtctg acaactttgg cgcgctcctg
     3241 gtaggttgct ccgaatgaaa ggtccccggc cttgatccgg ggaaggacct tcagtgtcat
     3301 ctttcccctg cgtaataaca ggttttcctt gggaagcaca tgcccgaagc cgtgaagcag
     3361 aaccggtgtt atatcaagct tcagttcttc agccagttgg aacgctccct tatgaaaccg
     3421 gagtatggag caatctgccg agcgggtacc ttctgggaag atcatcaaag aataaccctg
     3481 gctgatccgt tcgtgaattg tttcatggtt ggtgtccagg tattctatgg gaagataatc
     3541 ggcaaagcgg aggattgtcc tgtagaaagg agaattccat tcacggttgt tggtcattac
     3601 aatgatcttt ggatggagca tgatgatggc catcaggtcc aagtgggact ggtgattggc
     3661 aactatgacc gatggactgg aaaagcggtc ttcttcgcaa ttgataagtt ccatgcgtga
     3721 tcccggtata tggaagatca catatcggca catgccctgc agtatcctgt gaaagcggag
     3781 tttgtttctt tcgctgtgtc ctcccagcag cagcagaacc gcacccgata gagacaggaa
     3841 ggccactccg aacagaaaga acgtgaaaga aaaaaaggtc caccaggtgt tccacagggt
     3901 cagcggtgtc ttgcggtaac gtccccgctt ggttacaagt ttccggaaaa ggtatgccgg
     3961 caccgtgtag gaagtgatta ccaccgatat catacccaca atggttacct gggccagaga
     4021 acgcatggcc ggatgtttgg ccacgatcag ggaacctatt ccggcaagca tgatcaatgc
     4081 agacaaggca accgtattct tgtaggtgct gagcattttt ttgccatagg catactcgtg
     4141 catcatgcct tccgtgataa aaatcgtgta gtcatctccc agaccgaata tgaatgtggc
     4201 aaggataatg ttgactatat tgaattcaag gcccagcagg ctcatgatgc ccagtatcca
     4261 gatccagctg atggcaagcg gcaggaaggc tatcacgctc agctcaaggc gaccgaatga
     4321 aagggtcagg aacaggaaaa caataaaccc gcagatccac agcacgtaat caaaatcaga
     4381 ggaaagggaa gaaatcatga ggcgtgtgac agaacctgtg tcaaaggcaa aagcaggtga
     4441 ttccggtgtg ttgagctctg cttctatggc gggggcatcc agggcatcta catggagcat
     4501 agtgtagacc aggcttcttt ccggtttttc cgatatgtaa ttgacagcca ggttattcaa
     4561 aatagggccg aaatctttta gttcaccggt ggggtaggaa cgttcaagga tttcttcaaa
     4621 gcgggcgaac gatccttctc ggaatccgtt tttcctggcc agggcattga attgcaccag
     4681 gaacgggtct ttgcgtgtac tccagaaatc attccagcgt gcaatcctct cctgacgcat
     4741 ggaacgcgag ggcaggaaag cacctatgcc cgagtgccgg tagctgtcaa tttgtggccg
     4801 tacagcttca taattagcca gggcttcatc cagggtcgtt ccttctgnnn nnnnnnnata
     4861 cagaacgtgc tgtgaacctt gggtgcggtc caggagtttt tccatgcttt ccctttgcac
     4921 gggagtcata tagttaatgg actgcatgtc ggcattgaaa acaacaccct tgttgcccgc
     4981 ccagagcccc agggtcacga taagtaacgc aagcatgacc catcggttcc gttccgggga
     5041 ggaatctgcc agcatgccga atttcaatgt gttggataca ggaccggttg cccgtaccat
     5101 atgaggcaga aagataagga caaagataat ggtgcccatg aggagcaaag aggcaaacag
     5161 tccgaagcct ctcatggccg gtgagctaag gaacaacagg ctcagaaagg cacctacggt
     5221 ggttatgttg ccggtagtga gcgggggaac gatatcggca agggtctgcc tgggcgtata
     5281 tccctccctg atatgagaaa gaaaatggag cggatagttc accgctattc ccacgatgac
     5341 agaacctgcc ccgatggcaa taagggatag ggaggaggtg agcacgccgg ccatggaaaa
     5401 ggcaaaaagt gagcctgcca ggatggtagc gccaatgagc aatatctgtc ttgcattcct
     5461 gaaatagaag gccagaagta ccccgatgac cacaagtgct atggccaccg atatccaggt
     5521 gtctttcctt aagcggtctg catttgtcac agctatgtag gaagcaccga aaggagagat
     5581 cgtgacttca tggtcaaata cctgtaaggt ttcttcgata gcatcaccaa tggccccgat
     5641 cagttttttg ttatttccgg tatctcctgc agggtattgt gaatcaatgg taagaatgag
     5701 ctcggttcca tcaggggaga atatataatc cgagatgacc gtaaagtgct ccgagggctt
     5761 gaagccttcc agcctttgaa gcagtggccc tgaaaacagc aacggatctg acagaagggt
     5821 gttcctgagc atcattccgg ccggtgaaag cagggtctgc ctgacacgga ctatctgctg
     5881 ttccaggaca tctggctgca gaagggaatc aatgcgtgca tagtcccgtt cttccaggaa
     5941 gtaaggcatg ttcccggtta caaagtcgct gacctccagg gtaagcgcag ggtctgacat
     6001 ataggtcaca gcactgatat gttgacgggc agggtccttt tccaacaagc ctgccaggtg
     6061 gtcagccgca tccatgagaa ggccgctgtc tgtttccttc tcctggtctg ccatggcaat
     6121 gctgatgatg atcttgtcgg atccgcctat gttcctgtag gcatagccaa tccgttcatg
     6181 ttctttgcct gtggccaaaa atcccgagac gtcctcatca aaatccagca ggaaaaggcc
     6241 tcctgctgcc aggatgacgc ctaaagccag cagcacgtat agcgacctgg tgttcttgcg
     6301 gaaatgatcg tatattttca ggaacgtttg aaccatttat gcaggagtat tgatggataa
     6361 ccatatgcca gcgccgtcat cgtgagcact gtattgagga gcgatatgcg cagaaaatca
     6421 cggcctttcc tgaaatgcgt tacacgctct ccggcagggg cataaaagac cttaatgggc
     6481 agggggatca caggaatccc tttccaggca agccttacca ggatctcgag ttctgtctcg
     6541 taacggtttg tgaagaaacg cattttctgc atcctgtaca aggggtagag gcggtatccc
     6601 gtctgggtgt caggcaatcg ttcaaaggtc tgcaatgtga accagaaatt agaaaaccgg
     6661 tttgcaaagg tattttcccg gggcatgttt tcctgttcca gggaacggct tcccacaatg
     6721 agggcaccgg gatgctcctc catgactttg acaaatcgtg gtatctcgtc cggataatgt
     6781 tgtccgtccg catccagggt gatggcatga cgataaccct ttgctcctgc caatgcaaag
     6841 gcacgggaga gggcatatcc ttttcccctg ttggtagcgt atgatatgat ctctattttg
     6901 ccggcatagg tatccagcaa ctgcagggta ttatccgtgg aaccgtcgca gaccacgata
     6961 acatgactgc aatactccat ggtttgttct atgacccgga gtatggaaga cgcattgttg
     7021 tacgtcggaa tgatcacaca tacttcgtct ctttcagcca taaggtcatt ttagtcatga
     7081 cattgtcccc gtttttcact tctgtccgga ctttccacag tccctgtccc tgttccgtca
     7141 tctgcaacat gaaagtcaca cggggtgttt ccacgggatg gatgcccatc aggaacttga
     7201 tattatcggc tttttccagc aacaggccct tttccagtat aagcgatgcg atttcccgtc
     7261 ccatttcaac aatacaggct cccggggtga tgggattgcc cgggaaatgg gctttatata
     7321 tgacactatc gctatccagc tcaatggtca cccgtgcttc ttccccgtcg tagaccttat
     7381 caatgatctt gtataagtta ttgataaaca taagcatcaa aggttgtatc atcaagtggc
     7441 cggtttatcc tgatatttcc catcctgacc gttgtcttgt ctcccgaagt ctcgttgatg
     7501 acaacagtct ggatcacata atcctccttg cggaaaaaaa gttcaaggtc agtgaacaac
     7561 cgtttcatct cccgttgcga gggaatgagc ctgatgtgta tgtaatcttc tgacgggatg
     7621 tattcaggga caaaaagttc ccggtcttcc agtatgcttc cctctatccc catgagaatg
     7681 atagtgccga tatgcctgaa taaacccgcg gacccagcat cgcgcagggg ttttcccgaa
     7741 gggtccagta tggtcatttc cttcccgcgc aaaaggaaac cggaataatc cgggacctgg
     7801 tactgccaaa gcagcgcctt gtttttctca taggcaagct taccccgtga aatgcccttt
     7861 tcagccagta tggtgacttc ccgttcctgt tggaattcac attccagggt atggatgctt
     7921 ttgagtgcca ccatcatgtg gtgtctgttt tccactgtct gcggtatgac
//
LOCUS       DAOSOF010000091         7772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9362_length_7772_cov_4.764935, whole genome shotgun sequence.
ACCESSION   DAOSOF010000091 DAOSOF010000000
VERSION     DAOSOF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9362_length_7772_cov_4.764935"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1468
                     /locus_tag="PLM68_06045"
     CDS             5..1468
                     /locus_tag="PLM68_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT54960.1"
                     /translation="MDLQPQKDTWRIEKEIYGHFAEHLGTCIYGGLWVGPDSPIPNTQ
                     GYRNDVLEALKKLQIPVLRWPGGCFADEYHWRDGIGPRPLRPKMINTHWGGTVEDNSF
                     GTHEFLNLCELLDCEPYVSVNLGSGTVEEIADWVEYMTSPADSPLANLRRENGRDKPW
                     KVKYLGVGNESWGCGGNMRPEFYADLYRRYATYCRNFGDNRLFKVAVGPSDFDVNWTE
                     VMMERIGWRMNGLSVHFYTVYDWNAKGSATKFTPDDYYWTLGKCMDIDNVIKSHSAIM
                     DRTDPEKQIALMVDEWGAWWDEEPGTVRGHLYQQNTMRDAFVAALSLMVFHRHADRVK
                     MANIAQLANVLQAMVLTRDDKMVLTPTYHVFEMFKVHQGAFHVPMDVKTSDIVTEENK
                     VIQTVYATASVNEEGVMHISLANVSLDRSYEVTIHLNGAECRDISGRILTSARITDYN
                     DFDHPDTVRPVSFTGVKHGENSLILTIPAKSIIALEF"
     gene            1538..2827
                     /locus_tag="PLM68_06050"
     CDS             1538..2827
                     /locus_tag="PLM68_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54961.1"
                     /translation="MTTKKKSYALPIIMMIVLFGMISFVTNLAAPMGQVLKEQFNVSN
                     FQGLLGNAANFIAYAVMGIPGGMMLQRIGYKKTALIAIAIGFLGVLIQFLSGHSPESA
                     AFSVYLLGAFVAGFSMCLLNTVVNPMLNTLGGGGNKGNQLIQVGGSFNSLMATLTPAL
                     VGILIGEAAKANIKDVFPVLYIAMGVFALVFVVLLAVKIPEPHVAESKESLGKLMAGA
                     LKYRHFILGAIAIFVYVGVEVGTPGIMIYWLSDTPEVGKTIAGFVAGTYWFLMLIGRL
                     IGASVGNKVSSKAMLASTSLVGLGLILLALFSSTSTMVTLPVLQRSAIGGLSFGMADV
                     PINAMYIVLVGLCTSIMWGSIFNLAVEGLGKYTAAASGLFMVLVCGGGILPAFQGFVA
                     DKAGFIPSYWVVAIGLAYMLFYALAGSKIVNKEIQKQ"
     gene            2831..3817
                     /locus_tag="PLM68_06055"
     CDS             2831..3817
                     /locus_tag="PLM68_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT54962.1"
                     /translation="MVKPYVIGIDIGGQSTKLGVVDARGNIIIQDTFDTRDITTLKGY
                     MDELEKSLTRMIAVPEIGGKEKIKGIGIGAPNGNYYKGTIEAAPNLKWAKDKQGNPKV
                     IHLKNNVEEAMGLPAVVTNDANAAAQGEMIYGAAKGMKNFIEITLGTGVGSGIVIDGK
                     VVYGHDGFAGELGHTTSIRRTGRMCNCGKIGCLESYASAIGVARTARELLEMDPKRPS
                     TLRNMPADDITSRDVAEAAAKGDALSIEVFNYTGRILGEAFADFVAFSAPEAIVIFGG
                     LAQAGDLIFKPTIDAMEDNLLSIWKGKVKVIPSQLPGSEAAILGSAALAWEL"
     gene            3829..4983
                     /gene="galK"
                     /locus_tag="PLM68_06060"
     CDS             3829..4983
                     /gene="galK"
                     /locus_tag="PLM68_06060"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT54963.1"
                     /translation="MEIDIVQQQFREKFGDAPSRVYASPGRVNLIGEHTDYNGSFVFP
                     GAIDKGMVAVIRANGTKKVRAWSVDLQDHAGFTMDETDLPAASWARYIFGVCREIIKR
                     GGSPGGFDTAFAGDVPLGAGMSSSAALESTYAYALNDMFSLGIDRMELARIGQSTEHN
                     YVGVKCGIMDQFISLFGKKGNLIRLDTRNMEYKYFPFHPQGYRLVLLDTVVKHELASS
                     AYNKRRESCERVARLVRDSGYPHVEFLRDVTMDMLLEVKDRTTKEDFMRAKYVVEETE
                     RVLVVSDALERDDYETVGRMMYLTHYGMRDLYEVSCEELDFLNDVAKACGVTGSRVMG
                     GGFGGCTINLVKDHLYEGFLARAKEQFARKFGHQPKVYDVVIDDGARRIS"
     gene            complement(4980..5492)
                     /locus_tag="PLM68_06065"
     CDS             complement(4980..5492)
                     /locus_tag="PLM68_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOT54964.1"
                     /translation="MENRKDKVLKQLEDWNIAYRLYTHPPLPTAAEAMAYWKDIEAQH
                     CKNLFFRNHKGNRHYLVVFDWKRDLPIHRIEKMLRQGKLTFASPQRMMRYLGVEPGSV
                     SPFGLLNDMENHVVLFIDNKLKEAEEISFHPNDNRYTAVISKTDFFRFLELVGNAHVF
                     LDIDSPEILS"
     gene            5519..5992
                     /locus_tag="PLM68_06070"
     CDS             5519..5992
                     /locus_tag="PLM68_06070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT54965.1"
                     /translation="MNIRKATIKDADVLAANQVHMALESENIILDHQTVLVGTCAYLE
                     DENRGTAYIAEIKGEVAASLLVTREWSDWRNAWIWWIQSVYTVPKFRRMGIYKALYNH
                     LQELVKKSEDVIGIRLYVARNNPRAIETYRRLGMTDEHYTTFEWLRDPDTFKPAE"
     gene            5989..7101
                     /locus_tag="PLM68_06075"
     CDS             5989..7101
                     /locus_tag="PLM68_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT54966.1"
                     /translation="MKYFGFFISLLLVIYGLGNFYVLYRGYQWSPLHGPCLKWAFIIW
                     GIVMMLSLPLMNMFSLPCPRLQAFWMTISYSWLVFVLYALMLVLIADLTGGLFRLFHF
                     RDMISGTGRSILWFSVLALLLGLLLAGNFRYRHFTRKEMHVGLPGSVKMLAISDLHLG
                     LLTDNSRLEQFVGAINKEKPDVILVVGDLIDRSLEQVREQDMGSLLQKLEAPMGIYAV
                     PGNHEFYAKEDEALQWIAEQGIVVLRDSVVRIPGVAYILGREDHSVAGRKTLLQVWEN
                     SAFGSLERDLPLIVLDHQPLGIAEAVEYGVDFQLSGHTHAGQLWPVSLLVRKVNDLFY
                     GEYTRGKTRFYVTSGLGIWGPPFRIGVPRSEYVVIR"
     gene            complement(7202..>7772)
                     /locus_tag="PLM68_06080"
     CDS             complement(7202..>7772)
                     /locus_tag="PLM68_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT54967.1"
                     /translation="WSGNVTGFYSRISNWIAWLPTFKGFWSPVNVKQVKSYGIECRGS
                     LEIIPGHDWKIGGNGNFSWTRSLNLGDPVSWADEAIGKQLVYIPEFSAAFTGRVSWKE
                     WTFTYDWTYYSERYTTSSNMTRTQLGVLGPYFMNDIALEKSIRLNHAVLHVKAIVRNL
                     FNEAYETVLARPMPGINYTLSVGIVPLFR"
BASE COUNT         1913 a         1819 c         2119 g         1921 t
ORIGIN      
        1 cgtaattgac ctgcaaccgc agaaagacac atggcgcata gaaaaagaaa tctatggaca
       61 ttttgctgag cacctgggaa catgtattta cgggggcctc tgggtcggcc ccgattcccc
      121 tattccaaac acacagggat accgtaacga tgtgctggaa gccctgaaaa aactgcagat
      181 tccggtcctg cgctggcccg gaggttgttt tgccgatgaa taccactggc gagacgggat
      241 aggtccgcgg ccgctgagac ccaaaatgat caatacgcac tggggtggca cggtagagga
      301 taacagcttt ggcacccatg aattccttaa cctctgtgag ttattggatt gtgagccgta
      361 tgtgagcgtg aacctgggca gcggaacagt agaagaaata gccgattggg tggaatacat
      421 gacttccccg gccgacagtc ccctggccaa tctgcgacgg gaaaacggac gggacaaacc
      481 ctggaaggtt aaatatttag gcgtgggaaa tgaaagctgg ggttgcggag gcaatatgcg
      541 tcccgagttt tatgccgatc tatatcgcag gtatgccacc tattgccgga atttcgggga
      601 caaccgttta ttcaaagttg ccgtgggacc cagtgatttt gacgttaact ggacagaggt
      661 gatgatggaa agaataggat ggcggatgaa cgggctttcg gttcattttt acacggttta
      721 tgattggaat gccaaaggct ccgccacaaa gttcactccc gacgattatt actggacgct
      781 ggggaaatgc atggatatag acaatgtgat aaagtctcac agtgccatca tggaccggac
      841 cgaccccgaa aaacagattg cgcttatggt agacgaatgg ggagcctggt gggatgaaga
      901 acccggaact gtacgggggc atctgtacca gcagaatact atgcgcgatg cttttgtggc
      961 agctctttcg ctgatggttt tccaccgcca tgcagacagg gtgaaaatgg ccaacatagc
     1021 ccagctggcc aatgtactcc aggcgatggt tctgaccaga gacgacaaaa tggtccttac
     1081 tcccacctat catgtgtttg aaatgttcaa agtgcatcag ggcgcttttc atgtgccgat
     1141 ggatgtgaag acttcggata ttgtcactga agaaaacaaa gtgattcaga cggtatatgc
     1201 cactgcttct gtcaatgaag aaggcgtgat gcacatctct ctggccaacg taagcctgga
     1261 caggtcgtat gaggtcacga tccatttgaa cggagcggaa tgcagggaca tatcaggaag
     1321 aatcctgaca tctgccagga tcacggatta taacgacttt gatcaccctg atacagtcag
     1381 gcctgtttcc tttaccgggg tgaaacatgg tgaaaattca ctcatactga caatacctgc
     1441 caaatccatt attgcgcttg agttttgaaa agaaatcctt acttttgaca cgtttcgtta
     1501 aaaaaacgca caattaatta ctcataatcg ttcttatatg acaacaaaga aaaaaagcta
     1561 tgccctgcca atcatcatga tgattgtact gttcgggatg atctcatttg tgaccaatct
     1621 ggcagcaccc atgggtcagg tattgaaaga acaattcaat gtttctaatt tccaggggtt
     1681 gctgggtaat gcagccaatt tcattgccta tgccgtgatg ggaatacccg gcggtatgat
     1741 gttacagcgc ataggataca aaaaaacggc actcattgcc attgccattg gctttctggg
     1801 tgttctcatt cagtttctct ctggtcattc acccgaaagc gcagcattca gtgtatatct
     1861 tttaggcgca tttgttgccg gtttttcaat gtgcctgctc aatacggttg tgaatcccat
     1921 gctcaacaca ctgggcggag gcggaaataa aggcaaccag ctcatccagg tcggaggctc
     1981 tttcaattcc cttatggcaa cgctgactcc tgccctggtg ggtattctga ttggtgaggc
     2041 agccaaagcc aacatcaagg atgtatttcc cgtattatac attgccatgg gtgtatttgc
     2101 cctggtattt gttgtgcttt tggcagtaaa gattccggaa ccccatgtgg ccgaaagcaa
     2161 ggaatccttg ggtaaactga tggcaggagc cttgaaatac aggcatttca ttttgggtgc
     2221 catagccatt tttgtctacg tgggtgtaga agtaggaaca ccgggcatca tgatctactg
     2281 gctctccgac accccggaag tcgggaaaac cattgccggt ttcgtggcag gaacttactg
     2341 gttcctgatg ctgataggcc gtctgattgg ggcatcggta ggcaataagg tttccagcaa
     2401 agccatgctt gcttccactt cactggtggg attggggctg attcttctgg ccttattctc
     2461 ttctacttcc accatggtga cattaccggt gctgcagcgt tcagccattg gtggcctttc
     2521 cttcgggatg gccgatgtgc ccatcaatgc catgtatatt gtgctggtgg gtctgtgtac
     2581 ttccatcatg tggggcagca tcttcaacct ggctgtcgaa gggttgggca aatatacggc
     2641 tgccgcttcg ggccttttca tggtattggt ttgcggcgga ggcatcctgc ctgctttcca
     2701 gggatttgtt gctgacaagg caggctttat tcccagttac tgggtggtcg caattggcct
     2761 ggcctatatg ctgttctatg cccttgcagg atcaaagatt gttaataagg aaatccaaaa
     2821 acaataaatc atggtaaaac cttacgtaat cggcattgac attggaggtc agagcacaaa
     2881 actgggcgtt gtggatgccc gcggaaatat cattatccag gatactttcg atacccggga
     2941 tatcaccacc ctgaaaggct acatggatga actggaaaag agccttaccc gcatgatagc
     3001 cgtaccggag ataggcggta aggaaaagat caagggcatc ggcattggag cccctaatgg
     3061 caactattat aaggggacca ttgaagccgc ccccaacctg aaatgggcaa aagacaaaca
     3121 aggcaatccc aaagtgatcc acttgaagaa taatgtggaa gaagccatgg gtttgcctgc
     3181 agttgtcacc aacgatgcca atgcggcagc ccagggagaa atgatctatg gggcagccaa
     3241 aggcatgaag aatttcatcg agatcaccct gggtacagga gtaggcagcg gcattgtgat
     3301 agacggcaag gtggtatacg gacacgatgg ctttgcaggc gaactggggc ataccacatc
     3361 catccgccgt acaggccgta tgtgcaattg cggcaagatt ggttgcctgg aatcatatgc
     3421 ttcggccata ggtgttgcca ggacagcccg ggaactgctg gaaatggatc ccaagcgtcc
     3481 cagcaccctc aggaatatgc ccgcagatga tattacttcc agggatgttg ccgaggctgc
     3541 cgccaaagga gacgctttgt ccattgaagt attcaattat acgggaagga tcctgggaga
     3601 agcctttgcc gatttcgtgg ctttcagtgc accggaagcc atagtaattt tcggaggcct
     3661 tgcacaggcc ggtgacctga tattcaagcc caccatcgat gccatggaag ataacctgct
     3721 gtctatctgg aaagggaagg tgaaggtaat tccctcgcag ctgcccggat ccgaggcagc
     3781 catcctgggc tcggcagccc ttgcctggga attataaatg gtggcagtat ggagatagat
     3841 attgtacaac aacaattcag agaaaaattc ggtgacgctc cctcaagggt gtatgcttcc
     3901 cccggcaggg tgaacctgat tggagaacac accgattaca acgggagctt tgtctttccc
     3961 ggagccatag acaagggaat ggttgccgtg atccgcgcca acgggacaaa aaaggtacgt
     4021 gcctggtcgg tggatctgca ggatcatgcc gggttcacca tggatgaaac tgatcttccg
     4081 gccgcatcct gggccaggta tatctttggg gtatgccgtg agatcatcaa aaggggcggg
     4141 tcacccggag gatttgatac cgcttttgcc ggagacgttc ccctgggagc cggcatgtcc
     4201 tcgtccgcag ccctggaaag tacctatgcc tatgcattga atgatatgtt ttccctgggt
     4261 atagacagga tggaactggc ccgcatcggt cagtctaccg aacacaatta tgtaggggtg
     4321 aagtgcggca tcatggacca gttcatttca ctttttggga aaaaagggaa tctcatccgt
     4381 ctggacacgc ggaacatgga atacaagtat ttcccgttcc atccccaggg ttaccgcctg
     4441 gtgttgctgg ataccgtggt gaagcacgaa ctggcatcct cggcttacaa caagcgccgc
     4501 gaatcctgtg aacgtgtagc gcggctggtc cgtgattcgg ggtatcccca cgtggaattc
     4561 ctcagggacg tcactatgga catgctcctg gaggtgaaag accggacaac aaaagaagat
     4621 ttcatgcggg ccaaatatgt ggtggaagag acagaacggg tgcttgttgt gagtgatgcc
     4681 ctggaaaggg acgattatga aacggtgggc aggatgatgt acctgaccca ttacggcatg
     4741 agggatctgt acgaagtcag ctgtgaggaa ctcgattttc tcaacgatgt ggccaaggca
     4801 tgcggagtga ccggctcacg ggtgatggga ggaggattcg gagggtgcac catcaacctt
     4861 gtgaaggatc atttgtacga aggattcctt gcccgggcaa aagagcaatt tgcccgtaaa
     4921 ttcggtcatc agcccaaagt gtatgatgtg gtgatcgatg acggggcacg ccggatttcc
     4981 taagacagaa tttccgggct gtcaatgtcc aggaagacat gggcattgcc gaccagctcc
     5041 aggaagcgga agaagtctgt ttttgatatg actgccgtat accggttgtc attgggatga
     5101 aaactgattt cttccgcttc tttcagttta ttgtcgataa aaaggacaac atgattttcc
     5161 atgtcattca gcaggccgaa aggcgaaacc gaaccaggtt caacacccag gtagcgcatc
     5221 atccgctggg gtgatgcgaa agtgagtttg ccctgccgga gcattttctc tatacggtgg
     5281 atgggcaaat cccttttcca gtcgaaaacc accaggtaat gacggtttcc cttatgattc
     5341 ctgaagaaga gatttttaca gtgttgggct tcaatgtctt tccagtaagc catggcttct
     5401 gccgccgtgg gcaggggcgg atgagtgtat aaccggtacg caatattcca gtcttccagc
     5461 tgtttcagaa ctttgtcctt acggttttcc atgatgcaaa aatacttata tttgcccaat
     5521 gaacataaga aaagccacca taaaagatgc cgatgtactg gcggcgaacc aggtacacat
     5581 ggcacttgag tcggaaaaca tcatactgga tcaccagacg gtcctggtgg gaacctgtgc
     5641 ttacctggaa gatgaaaacc gggggactgc ctacatcgcg gaaatcaagg gagaggtagc
     5701 agcctcgttg ctcgttacca gggaatggag tgactggcgc aatgcctgga tatggtggat
     5761 acaaagcgta tacactgttc ccaaattccg caggatgggt atctacaagg cactctacaa
     5821 ccatttgcag gaactggtca aaaaatctga agatgtgatc ggtattcgcc tgtatgtggc
     5881 caggaacaat ccccgggcaa tagaaacata ccgaaggctg ggcatgaccg acgaacatta
     5941 caccaccttt gagtggctca gggacccgga cacgttcaaa cccgcagaat gaaatatttc
     6001 ggattcttta tttccctgct cctggtcatc tatggcctgg gtaactttta tgtgctttac
     6061 cggggttatc aatggtcacc cttacacgga ccctgtctga aatgggcatt cattatctgg
     6121 ggcattgtca tgatgctctc tttacccctg atgaacatgt tttcccttcc ctgcccccgg
     6181 ctgcaggcct tttggatgac catttcctac tcatggcttg tctttgtcct ctatgccctt
     6241 atgctggtat tgattgccga tttgaccggg ggcctgttcc ggttattcca tttccgtgat
     6301 atgatctcgg gaacggggcg ttccattctg tggttttccg ttctggccct tttgctgggt
     6361 ttgttgctgg ccgggaattt caggtacagg cattttaccc gcaaagaaat gcatgttggt
     6421 ttgcccggct ccgtcaaaat gttggccatc agcgatctgc atctgggttt gttaacggat
     6481 aactcgcggt tggagcaatt cgtcggggcc atcaacaagg aaaaaccgga tgtaatcctg
     6541 gtagtggggg acttgattga ccgctccttg gagcaggtga gggagcagga catgggttcc
     6601 ctcctgcaga agctggaagc gcccatggga atctatgccg tgcctggtaa ccatgaattt
     6661 tatgccaagg aggatgaggc gctccaatgg attgccgaac aggggatcgt ggtcttgagg
     6721 gattcggttg tccggatccc cggcgtggct tacatcctgg gcagggaaga ccactcggtg
     6781 gcaggaagga aaacactcct ccaggtgtgg gaaaactcgg cttttggctc cctggaaagg
     6841 gatttacccc tgattgttct ggaccaccag cctttgggaa ttgccgaggc tgttgaatac
     6901 ggggtggact tccagctctc cggacatact cacgctggtc agctgtggcc tgtttccctg
     6961 ctggtccgga aggtgaacga tcttttttat ggagaataca cccgcggaaa aacccggttt
     7021 tatgtcacat cggggctggg tatctggggc ccgccattcc gcatcggggt gcctcgcagt
     7081 gaatatgtgg taatccggta acaagtggta atccggtaac aagtggtgat ccggtaacaa
     7141 gtggtgatcc ggtaacaggt ggtgatccgg taacaggtgg tgaccggcat aaagaagatg
     7201 gttaccggaa aagggggacg atcccgacag agagtgtgta attgatcccg ggcatgggtc
     7261 ttgccaggac ggtttcatag gcttcgttga agaggtttct aacgattgcc ttcacatgca
     7321 ggactgcatg gtttagccgg atactttttt ccagagcaat gtcattcatg aaataggggc
     7381 ccaaaactcc cagctgtgtg cgcgtcatgt tgctcgaggt ggtataacgc tcgctgtaat
     7441 aagtccagtc gtaggtaaag gtccactcct tccagctgac cctccctgta aaggcggcag
     7501 aaaactccgg aatatagacc agttgttttc cgatggcttc gtctgcccag ctaacgggat
     7561 cccccaggtt caaagacctg gtccaggaga aattgccgtt tcctccaatt ttccagtcat
     7621 ggcccggtat tatttccagg gaacccctgc attcgatccc gtaacttttc acctgtttaa
     7681 cattcacggg agaccagaat cccttgaatg tcggcaacca ggcaatccag ttgctgatac
     7741 gggagtaaaa accggtgacg ttcccgctcc aa
//
LOCUS       DAOSOF010000092         7648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9577_length_7648_cov_3.790860, whole genome shotgun sequence.
ACCESSION   DAOSOF010000092 DAOSOF010000000
VERSION     DAOSOF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9577_length_7648_cov_3.790860"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="dnaJ"
                     /locus_tag="PLM68_06085"
     CDS             complement(<1..972)
                     /gene="dnaJ"
                     /locus_tag="PLM68_06085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT54968.1"
                     /translation="MAKRDFYEVLGVEKSASAEEIKKAYRKKALQYHPDKNPGNKEAE
                     EKFKEAAEAYDVLSDQAKKSRYDQFGHAGVDGMGAGGPGGFAGGFTMDDIFSRFGDIF
                     GGAFGGSPFGSPFGSQAGTRRGVRRGSDIRIRVKLNLEEIAHGADKVVKINKLKSCSH
                     CGGKGAVSESDMRKCPTCNGSGVFTRVTHTFLGQMQNSSPCPQCNGEGKIVTNPCNEC
                     KGEGVVKQAEEISFSIPKGVEEGMQLTISGKGNAPKRGGVNGNLLVVIEEEPHPQLQR
                     DGHDLIYTLFLSVVQAALGDEVEIPTVDGKAKIKIEQGIQSGRILRLR"
     gene            complement(981..1577)
                     /locus_tag="PLM68_06090"
     CDS             complement(981..1577)
                     /locus_tag="PLM68_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT54969.1"
                     /translation="MQADKKKHQAQEGPDVPHVEERDNVSGANGQAPAQEEPGTGIAQ
                     AEETPDVNKLKEQLDQTNDRLLRLHAEFDNYRKRTAKERLDLVLTASEDVIKGMLPVL
                     DDCQRALRTLEKLENADPVSVEGVNLIYSKLLTYLQGRGLKIIEAKGKPLDTDFHCAV
                     AKTPVKEKKLKGKIVDVVMEGYTLNGKVIRFANVVVGE"
     gene            complement(1579..3501)
                     /gene="dnaK"
                     /locus_tag="PLM68_06095"
                     /pseudo
     CDS             complement(1579..3501)
                     /gene="dnaK"
                     /locus_tag="PLM68_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            3619..4317
                     /locus_tag="PLM68_06100"
     CDS             3619..4317
                     /locus_tag="PLM68_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOT54970.1"
                     /translation="MSYHVQTTLSERILYEDNHLIVINKMVGEIVQGDITGDTPLSEA
                     IKAFLKVRDAKPGNVFLGVPHRLDRPVSGICLFAKTSKALERMNRMFNQGTVSKTYLA
                     LVANKPAQDEALLVHYLTRNEKQNKSYALDEPEQGAKEARLRYKYLKSIRNYHLLEIE
                     LLTGRHHQIRCQLAAMGSPIKGDLKYGASRPNQDGGISLHAFRLAFVHPVTREPVVFV
                     SPWSVFGKKVTDSQ"
     gene            complement(4301..5773)
                     /locus_tag="PLM68_06105"
     CDS             complement(4301..5773)
                     /locus_tag="PLM68_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT54971.1"
                     /translation="MKVFRTKDIGTVDLLYREITGVTEQDLIERAAHAFVQAFIREEK
                     KPGRVLVVAGPGNNGADARSVARLLTELGWQTDLWDFSEKDQVENQLADWTGYLQNLP
                     AGTVVIDGLFGSGLTRPVTDPYARIIEGINALDTTVYAVDIPSGLAGDGIPFTTGAVV
                     KASATFTFQFPKLSFFFAENYPYVGQWEVLDIGMGGMAYPEVDTPFRMTEESDIHNLF
                     PRKRHPFSFKNNYGHALLIAGSLGKMGAAILSARSCLRVGTGLLTAHLPGRGEIPMQT
                     SLPEAMVSLDSHRDVAGDLPHGLLVPKTPYTAIGAGPGLGTSGETAGMLWDLLKWFGN
                     KTPCPSLVLDADALNIISEHPGMMEYLPRGTVLTPHPGEFDRLARACGMARAESSYLR
                     AIQAVEMAVEHKIIVVLKGRYTLIATPTAPHWFNPAGNPAMATAGSGDVLTGIILGLL
                     AQGFEPRTAAILGVWLHARAGDKAAATDRFLLAGDIIENL"
     gene            complement(5784..6341)
                     /gene="nadD"
                     /locus_tag="PLM68_06110"
     CDS             complement(5784..6341)
                     /gene="nadD"
                     /locus_tag="PLM68_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOT54972.1"
                     /translation="MNTCGLYFGSFNPFHIGHLAIVNYLVSFTTLDSVRLVVSPLNPL
                     KFDREPCFQSPRERLLHIRKAMETRKLPVEVSDVEFNMPRPLYTINTLHHLQVQEPGV
                     NFILVIGADNLEHIESWKSWETILEEFILYIYPRQDVDSIALREKYISRACGIVLLRA
                     PLIAISSTFIREGLEQGKNMNGFLV"
     gene            complement(6343..6909)
                     /gene="gmk"
                     /locus_tag="PLM68_06115"
     CDS             complement(6343..6909)
                     /gene="gmk"
                     /locus_tag="PLM68_06115"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT54973.1"
                     /translation="MSDKKALIFSAPSGAGKSTVVNHLMEIFPRLDFSITATTRPKRG
                     TEENGKEYYFISREEFLRLIEEDAFVEWQEVYKGSYYGTLKLELERIWNGNKVAVFDV
                     DVKGGINLKKIFGNDALSVFVAPPSLEVLRERLVKRGTDSPRAIEERLAKATLEMEDA
                     RNFDVVLVNDRLEDTLDKATEIVAAFIY"
     gene            complement(6899..>7648)
                     /locus_tag="PLM68_06120"
     CDS             complement(6899..>7648)
                     /locus_tag="PLM68_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT54974.1"
                     /translation="EETNWHTVKELDYRVALLPWGATEAHNHHLPYGSDTIMATHVAR
                     CSAAIAWERGVRTLVLPQIAYGVNSGQMDIPFCMHMNPSTQQRILEDIVAVLVRHKVN
                     KLVIVNGHGGNMFQNAIRELELRFPGMLICMLNWWRVRPVKEYFDNPGDHADEMETSA
                     MMAIRPELVLPLEYAGSGKEHRFTIKGFRERWAWISRRWIYTTDDTGVGDPSLSTPDK
                     GKRFMDNCIAEVAAFLEEFSKIGKMEDLYER"
BASE COUNT         1621 a         2135 c         1829 g         2063 t
ORIGIN      
        1 cctcaaacgc aagatacggc cactctggat accctgttct attttgatct ttgccttccc
       61 gtctacggtg gggatttcca cttcgtctcc cagcgcagcc tgcacaactg acaaaaagag
      121 cgtgtagatc agatcgtgcc cgtcacgctg caattgcgga tggggctctt cttctatgac
      181 aaccagaagg tttccgttga cacctccacg tttgggagca tttcccttgc ccgagatggt
      241 aagctgcatc ccctcctcca cccctttggg tatggaaaag gatatttcct cggcttgctt
      301 tacgacacct tctcccttgc attcattaca gggattggtg actatctttc cctcgccgtt
      361 gcactgaggg catggactgc tgttctgcat ttgtcccagg aaagtatgcg tgacccgggt
      421 aaatacccct gacccattac aggtgggaca ttttctcatg tcagactcgg atacagcgcc
      481 ttttccgcca caatggctgc aggatttgag cttgttgatc ttgacaacct tgtcggcccc
      541 gtgggcaatc tcttcaaggt tcagcttcac ccgtatgcgg atatccgaac cacgccgcac
      601 cccgcgtctt gtgccggcct gactgccaaa gggactgcca aagggacttc ctccgaatgc
      661 cccgccgaag atgtctccaa accgggagaa gatatcgtcc atggtgaacc cgcctgcaaa
      721 acctcccggc cctccggcac ccattccatc cacacctgca tggccgaact ggtcgtatcg
      781 cgacttcttg gcctgatcgc tcagcacatc gtacgcttcg gctgcttcct tgaatttttc
      841 ttctgcctcc ttgtttcccg ggttcttgtc cggatggtac tggagtgctt ttttccggta
      901 agctttcttg atttcttcgg cagaagcgct cttctctacg cccaatactt cgtaaaagtc
      961 tctttttgcc atattgtatc ttattcgccc actaccacgt tagcgaacct gattactttt
     1021 ccgttcagag tatatccctc cataacaaca tccactatct ttcctttcaa ttttttctct
     1081 tttacgggag tttttgccac agcacaatga aaatccgtat ccagcggttt tcctttagcc
     1141 tctatgatct tcagtcccct tccctgtaaa taggtcagca atttgctgta tatcaaattc
     1201 acaccttcca ccgatacagg atctgcgttc tccagttttt ccagtgttct gagtgcgcgc
     1261 tggcaatcat ccagcaccgg gagcatgccc tttataacgt cttcggaagc tgtcagaacc
     1321 aggtccaggc gttcctttgc cgtacgtttg cggtaattgt cgaattccgc atgcaacctg
     1381 agcagtctgt cgtttgtctg gtccagttgc tcctttaact tgttcacgtc gggtgtctct
     1441 tccgcctgtg ctatcccggt acccggttct tcctgagcgg gtgcctgacc atttgctccg
     1501 gatacattat ctctttcctc aacatgagga acatcaggcc cctcctgggc ctgatgtttc
     1561 tttttgtctg cttgcatatt acttgacctc ttcaaaatct acatccttga cttctccttc
     1621 ttcctgtgtg gcgccagtcc cattgcctgg tgcgcctcct tcaaaggggc tctgctgttg
     1681 gccttcaccg gaagcctagg aagctttgta catttcttcc gaggcagcat gccatgcctt
     1741 attgaggttt tccatagcac tgtctatggc tgcaatatcc tgtgatttat gtgcctcttt
     1801 caattgattc agggcttcct gtatgggatt cttcttgtct tccggcaatt tgtccccata
     1861 ctccttgagc tgtttctctg tctggaagat cagactgtcg gcttcgttga tcttatcgac
     1921 acgttccctt tccttcttgt cggcttcttc attggccttt gcctcatccc gcattttttt
     1981 gatctctgct tcggtaaggc ccgaggaagc ctcaatgcgg attttctgtt ccttgcccgt
     2041 ccccttgtcc tttgccgaga cgttcaatat gccgttggca tctatgtcga aggacacttc
     2101 tatctggggt atgccacggg gagccggagc tatgccatcc aggtggaatc gtccgatggt
     2161 cttgttatcc cttgccatgg gcctttctcc ctgcagcacg tggatctcta cggaaggctg
     2221 gttgtcgctg gcagtggaga aaacctgtga tttttttgtg ggtatggttg tgttggcttc
     2281 tatgagcctt gtcataactc ctcccagggt ttcaatgcca aggctcagcg gggtgacgtc
     2341 cagcaaaagc acgtccttca catctcccga caaaacaccg ccctggatgg cagcgccaat
     2401 ggcaaccact tcgtccggat tgacactctt atcgggaacc tgcccgaaca gtttttgcac
     2461 cagctgctgt atgaccggta tacgggttga accacccacc agtatcactt catcgatatc
     2521 ggaaggtttc atgcctgcat ctgaaagtgc tttcttgcag ggttccacgg tgcgctgcac
     2581 caggctgtct atgagttgtt caaattttgc ccttgtcaat gtcttgacaa ggtgtttggg
     2641 catgccgtcc acaggcatga tatacggcag gttgatctct gtggagttct ggctggagag
     2701 ttcgatcttg gccttttcag ctgcttcttt caggcgctgc agggccatgg gatccttcct
     2761 taggtccagt ccgctgtttt ccgaagcaaa ttccccggca agccagtcaa tcactgcctg
     2821 gtcaaagtca tcgcctccca ggtgggtgtc cccgttggtg gatttgacct cgaacactcc
     2881 gtctcccagt tccaggatgg atatgtcgaa tgtgccacct cccaggtcat acacggcgat
     2941 cttcatgtcc ttgtgtttct tgtccagtcc ataggccaac gaagcagccg taggctcgtt
     3001 tatgatacga cgaaccttga gtccggcgat ttctccggcc tctttggttg cctgcctttg
     3061 tgaatcactg aaataggcag ggacggttat cacggcttcg gtcacttcct gtcccaggaa
     3121 atcttctgcc gtcttcttca tcttctgcaa gaccatagct gatatttcct gcggtgtata
     3181 taaacgttcg tcaatcttca cccggggtgt gttgttgtct cccttttcca cggcatacga
     3241 aaccctgttg cgctcaaggg caaccttgtc gaaagtttct cccatgaaac gcttgatcga
     3301 ataaatggtt tttagggggt ttgtaattgc ctgacgtttt gcgggatcac ctatcttgcg
     3361 ttcgccattg tctgcaaatg ccacaaccga aggggttgtg cgttttcctt ccgaattgat
     3421 gatcactacc ggttcgttgc cctccatgac ggcaacgcag gagttggtgg tccccaggtc
     3481 tataccaata attttaccca taatttattt gttctttaat gattatttac tgatttctgc
     3541 cctatcatgc tcaatagtta tgccaaaagg catttgctga caggttggca gttcatggga
     3601 ttcattatct ttgtcactat gtcatatcat gttcagacga cgctgtcaga gcgcatactg
     3661 tacgaggata accaccttat tgtgatcaac aagatggtgg gcgaaatcgt tcagggagac
     3721 ataacgggag acaccccgct gagtgaagcc atcaaagcct tccttaaggt acgggatgca
     3781 aagcccggga atgtctttct tggcgtaccc caccggctgg accgtccggt gagtggtatc
     3841 tgtctgtttg ctaagacgtc caaagcccta gagcgcatga acaggatgtt taaccagggt
     3901 accgtcagca aaacatacct ggctcttgtg gcaaacaaac ccgcacagga cgaggcactc
     3961 ctggtccatt acctgaccag gaacgagaaa cagaacaaat catatgccct ggatgaaccg
     4021 gagcaagggg ccaaagaagc caggttacgc tacaaatacc tgaaaagcat ccggaattac
     4081 catttgctgg aaatagaact tctgacagga aggcaccacc agatccgctg tcagctggcg
     4141 gccatgggtt cccccataaa aggcgacctc aaatacggcg cttcacgtcc caatcaggac
     4201 ggtggcattt ccttgcatgc ctttcgcctg gcatttgttc atccggtaac aagggagccc
     4261 gttgtattcg tgtccccgtg gtctgttttc gggaaaaaag ttacagattc tcaatgatat
     4321 cgccggcaag gagaaatcta tcggtggcag cagccttgtc tcctgccctg gcatgaagcc
     4381 atacacccag tatggctgcc gtccggggtt caaatccctg tgctagcaat cccaggatga
     4441 tccctgtcag gacatcaccg cttccggctg ttgccatggc cggatttccg gcgggattga
     4501 accagtgagg ggcggtcggt gtcgcaatca gggtgtacct tccctttagc acaacaatga
     4561 tcttgtgttc caccgccatt tcaacggcct gtatggccct caggtaagag ctctcggcac
     4621 gggccatgcc acaggccctg gccagacggt cgaattctcc cggatggggc gtcaggaccg
     4681 ttcccctggg caggtattcc atcatgccgg gatgttcgga tatgatgttc agggcatctg
     4741 catccagcac cagggagggg caaggtgtct tattgccgaa ccatttcagc aaatcccata
     4801 acataccggc cgtttccccg gatgttccaa ggcccggtcc ggctccgatg gctgtatagg
     4861 gtgtctttgg caccaggagt ccatgcggca ggtctccggc cacgtccctg tggctgtcca
     4921 gggaaaccat ggcctcgggc aatgaggtct gcatggggat ctctccccgt ccgggaagat
     4981 gtgccgtcag aaggccggtg cccacacgca ggcaagaacg ggcagaaaga atggcggctc
     5041 ccattttacc caggcttccg gcaatgagca gggcgtgccc gtagttgttc ttgaaagaaa
     5101 aagggtgcct tttccgggga aaaaggttat ggatatcaga ctcttctgtc atcctgaacg
     5161 gtgtatcaac ctctggataa gccatccctc ccattcctat atcaaggacc tcccactggc
     5221 ccacataagg gtaattctcg gcaaagaaga aagacaattt agggaactga aaggtgaaag
     5281 tggccgaggc tttcacaacc gctccggtgg taaagggaat gccgtcaccg gcaagtccgg
     5341 aaggaatatc cacagcatac acagtggtat ccagcgcatt gataccctca atgatccggg
     5401 cataaggatc tgtcacaggc cttgtcaggc ccgatccgaa taatccgtct atcaccaccg
     5461 tgcccgcagg caggttttgc aagtaaccgg tccagtctgc cagctgattt tccacttggt
     5521 ctttttctga aaaatcccac agatctgtct gccagcccag ttcggtcaac aagcgtgcaa
     5581 cggaccgggc atcagccccg ttgttgcccg ggccggcaac caccagtacc cttcccggtt
     5641 tcttttcctc tcggatgaaa gcctgcacaa aagcgtgtgc cgcacgctct atcaggtctt
     5701 gttcggtcac acccgttatt tccctgtaca aaaggtctac ggtgccgatg tcctttgtgc
     5761 ggaaaacttt cataacctgt atatcagact aaaaaaccgt tcatgttctt tccctgttcc
     5821 agaccttccc taatgaaagt ggaggagatg gctatcagcg gagcccgcag cagtacaatg
     5881 ccacaggcac gggaaatgta cttttccctc aaagctattg agtccacgtc ctgtcgtggg
     5941 taaatataaa ggatgaattc ttccaggatg gtttcccagg atttccatga ttcaatatgc
     6001 tccaggttgt cagcacctat caccaggatg aaattcaccc cgggttcctg cacctgcaga
     6061 tgatgcaacg tgttgatagt ataaagcggc cggggcatgt tgaattccac atcggacact
     6121 tctaccggca gcttcctggt ttccatggcc ttgcgtatgt gaagcaatcg ctcccgcggt
     6181 gattgaaagc agggctccct gtcgaactta agcggattga gcggcgatac aaccagccgg
     6241 acactgtcca gcgtggtaaa ggacaccaga tagttcacaa tggccaggtg ccctatgtgg
     6301 aagggattga aagaaccaaa atataagccg caggtattca tgtcaataaa taaaagctgc
     6361 cactatttcc gtggctttgt ccaaggtgtc ttccagacgg tcattgacca ggaccacgtc
     6421 aaaattgcgt gcatcctcca tctctaaggt agccttggcc agccgttctt ctatggcccg
     6481 gggcgaatct gttccccttt ttacaagccg ttcgcgcaat acttccagcg aaggcggggc
     6541 aacgaacacc gatagggcgt cgttcccgaa tatttttttc aggttgatac cccctttaac
     6601 atctacgtca aaaacagcca ctttgttccc gttccatatc ctttcaagct ccagtttcaa
     6661 agtgccgtaa taactgccct tatacacttc ctgccactca acgaaagcat cctcttcaat
     6721 aagcctgaga aactcttccc tgcttatgaa gtagtattct ttcccgtttt cttctgtgcc
     6781 gcgttttggc ctggtggtgg ctgtaatgga aaaatccaga cgtggaaaga tttccatcag
     6841 gtggttcacc acagtggatt ttcctgcgcc cgatggtgct gaaaatatca atgctttctt
     6901 atcgctcata caaatcctcc attttcccga tctttgaaaa ttcttccagg aaagcggcca
     6961 cttcggctat gcagttatcc atgaaacgct tgcccttgtc cggagttgac agggaaggat
     7021 cccccacccc cgtatcgtcc gttgtataga tccagcgcct tgaaatccag gcccatcttt
     7081 ccctgaatcc cttgatggtg aaacggtgtt ccttgccgct tcctgcatat tccaggggaa
     7141 gcaccagctc aggccggatt gccatcatgg ccgaggtttc catttcatcg gcatggtctc
     7201 ccgggttgtc aaaatactct ttcaccgggc ggacacgcca ccagttcagc atgcatatga
     7261 gcatgccggg aaaacggagt tccagttccc tgatggcatt ttggaacatg tttccgccat
     7321 gcccgttgac tatgaccagc ttgttcacct tgtggcgcac caggacggca actatgtctt
     7381 ccagaatgcg ttgttgcgtg gaaggattca tgtgcatgca aaaagggata tccatctgtc
     7441 ccgagttcac cccataggcg atctggggga gcaccagggt cctcacaccc cgctcccagg
     7501 caatggcggc gctgcagcgt gcaacatgag tggccatgat ggtatcgctc ccgtaaggca
     7561 ggtgatgatt atgtgcctcg gtagcccccc agggcaacaa cgcaacccgg taatccagtt
     7621 ctttcacagt atgccagttt gtttcttc
//
LOCUS       DAOSOF010000093         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9608_length_7626_cov_4.558315, whole genome shotgun sequence.
ACCESSION   DAOSOF010000093 DAOSOF010000000
VERSION     DAOSOF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_7626_cov_4.558315"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="tuf"
                     /locus_tag="PLM68_06125"
     CDS             complement(<1..947)
                     /gene="tuf"
                     /locus_tag="PLM68_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT54975.1"
                     /translation="MAKETYKRDKPHVNIGTIGHVDHGKTTLTAAITQVLAKKGLSEI
                     RSFDSIDNAPEEKERGITINTSHVEYQTASRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTNEHVLLARQVNVPQIVVFLNKCDMVDDPEMLDLVELEVRDLLSK
                     YNFDGDNAPVIRGSALGALNGEAQWEEKVMELMDAVDSYIPIPLRENEKPFIMPVEDI
                     FSITGRGTVATGRIETGVIKTGDQVEIVGLGEKPKTSVVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKKEVKRGQVICRPGTITPHKKFSAGIYVLKK"
     gene            complement(984..1055)
                     /locus_tag="PLM68_06130"
     tRNA            complement(984..1055)
                     /locus_tag="PLM68_06130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Thr,seq:ggt)
     gene            complement(1071..1146)
                     /locus_tag="PLM68_06135"
     tRNA            complement(1071..1146)
                     /locus_tag="PLM68_06135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1111..1113),aa:Gly,seq:tcc)
     gene            complement(1153..1235)
                     /locus_tag="PLM68_06140"
     tRNA            complement(1153..1235)
                     /locus_tag="PLM68_06140"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1199..1201),aa:Tyr,seq:gta)
     gene            complement(1266..1339)
                     /locus_tag="PLM68_06145"
     tRNA            complement(1266..1339)
                     /locus_tag="PLM68_06145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Thr,seq:tgt)
     gene            complement(1448..3328)
                     /gene="dxs"
                     /locus_tag="PLM68_06150"
     CDS             complement(1448..3328)
                     /gene="dxs"
                     /locus_tag="PLM68_06150"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT54976.1"
                     /translation="MDNLIEQIDSPEDLKKLKPEQLPQLCAEIRKYITECCSHNPGHL
                     GASLGTVELTVALHYVYDAPKDKIIWDVGHQAYTHKILTGRRKEFLRNRCYNGLSGFP
                     KMDESPYDAFGTGHSSTSISAALGYATAAAMRKSDEKTVAVIGDGSMTAGLAYEGLNN
                     AGGCNADMLIVLNDNKMSIEPNVGGLHNSLLKLTTSGKYNRLKMKTWKALGSGTIRDA
                     IQETVKAGKRLFVKNSTLFQPLGIRYFGPVDGHDVILLVNTFKRLKNLKGPRLLHALT
                     IKGKGYAPAENDQPTWHAPGLFDAETGHRLTDDDPDVPTKTRFQDVFGQTLLELARTN
                     PDITGITPAMPSGCSMNLMMEEMPHRCFDVGIAEQHAVTFSAGLAAAGFLPYCNIYSS
                     FMQRAYDSVIHDVAIQNLKVVFCLDRGGLVGEDGVTHHGAYDLAYLNCVPGLIVAAPM
                     DEVELRNMMYSAQDKRYTATSIRYPRGTGAGIKDWRRPFQYLEPGRARLLSQGKDMAL
                     LSLGAAGIMGAAAVAKAAEKGISVQHYDMRFLKPIDGEVLEYVSRNFSKVITLEDGCL
                     IGGLHSTVSTYLARHKEAISKVTGLGIPDRFIQHGKTKQLYHECGYDTESILQAIIAL
                     AG"
     gene            complement(3336..4601)
                     /locus_tag="PLM68_06155"
     CDS             complement(3336..4601)
                     /locus_tag="PLM68_06155"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT54977.1"
                     /translation="MNIDIVLGLQWGDEGKGKIVDYLAQRYPVIARFQGGPNAGHSLH
                     FEGSSFVLHSVPAGIFRSNTMNIIGNGVVIDPVIFGEECRSLEALGIPVRDRLMISKR
                     AHLILPTHRIIDAASEHSKGASRIGSTLKGIGPAYMDKTGRNGIRVGDIIAPDFRDRF
                     LALKSKHIAFLRQFDYAYDADQGEDNWLNAIETMKQFPLIDAEYYLSNCLKENKRVLA
                     EGAQGTLLDIDFGSYPFVTSSSTTCAGACIGLGFPPTRVGRVYGIFKAYCTRVGSGPF
                     PTELNDRTGEIIREQGKEFGATTGRPRRTGWLDLVALKYAVILNGVTDLIMMKADVLD
                     AFDTIKIATSYTVQGQEINRVPFDTHTPITPVYREFRGWGKKLSALRKEEELPYELMT
                     YVRFIEDATGVPVKIISLGPDREQTIIRD"
     gene            4688..6130
                     /locus_tag="PLM68_06160"
     CDS             4688..6130
                     /locus_tag="PLM68_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54978.1"
                     /translation="MTQVKKLLNDSFVARWAALLLIALMMFFAYMFVDVMSPLQTLVE
                     GQRGWTPGIFGTYAASEYFLNVCGFLILAGIILDKSGIRFTGALSASLMVIGASIKYI
                     GISDWFQETELCAWLNTWWVSLPGSAKIASLGFMIFGCGCEMAGITVSKAIAKWFKGK
                     EMALAMGLEMAIARLGVFTVLSLSPFIANKFNGSVVAPIAFCTVLLLIGLINFLVFNV
                     MDAKLDRQMGDARGNEAPAEEFKVSDIKNIFGSKMFWIIALLCVLYYSAIFPFQRYAT
                     NFLECTLSIPTESAANLFRWFPILAMVLTPFLGSFIDHVGKGASMLMYGALIMIICHL
                     TFAFVLPLYPKEWFALLIIVVLGVSFSLVPASLWPSVPKIIDERYLGSAYSLIFWIQN
                     FGLCFVPKIIGNVLDSTNPGVAQLREQANAQLAQGIQAVVPAYDYTVPMVIFASFGVL
                     AFILGLWLKAEDRAKGYGLELPNITNKVKK"
     gene            6130..7539
                     /locus_tag="PLM68_06165"
     CDS             6130..7539
                     /locus_tag="PLM68_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT54979.1"
                     /translation="MNKVVKLLRDSRWVRWAVLLCLSLVMFASYFFDDQFTTIGDIFK
                     NPAILDLSWTSGDYGMYGGAYSLLCVWGGLIICGMLLDKWGVRVTGTLFVLLMVGGAA
                     IVVYGISASFNDSGLYHVMARAFDKPSLAMAYAGCAVFGLGSEIAGVAVTRSIAKWFR
                     GKEMALAMGLQLALARLGTATALIVVPRIVNLNDTLIPFSETSKPALAGFILLLCGMV
                     VWGVFLAFDKKLDTQTAAEALKNSQPVSKEDKFKFSDIFKILGNKHYILISLLCVFFY
                     ACIISFRRFATSIIIPRFEIEDNIASLMVALIPFTTMIFTPIFGSLVDFRGKATRWMI
                     LGSCLVLVAHLIIGFAPGLPVFGYLGIAILGIGYSLVPAAMWPSVPKIIPEKNLGTAY
                     SLIYWIQNMGMLLVPIFVGQIIEKNINDQIRAAVQAEYLFIGLALVAIAVSLILSRSA
                     DKNPQLQLDDPNKVRKTVN"
BASE COUNT         1772 a         1873 c         1845 g         2136 t
ORIGIN      
        1 tctttcttaa gtacgtaaat acctgcggag aacttcttat gaggggtaat tgttcccggc
       61 ctgcagatta cctggccgcg ttttacctct ttcttgtcta tcccgcggag caacagccca
      121 acgttatcac cggcctcacc cctgtcaagt atcttgcgga acatttcaac acctgttaca
      181 acgctggttt tgggtttttc accaagaccc acaatctcta cctggtcacc ggttttgatc
      241 acaccggttt caatacggcc ggtagcaacg gtaccacgtc ctgtgattga gaaaatgtct
      301 tctacgggca taatgaaagg tttctcgttt tcacgaagtg ggatcggaat atacgagtca
      361 acagcatcca taagttccat gactttttct tcccactggg cttctccgtt aagggcaccc
      421 aaagcagaac cgcggataac gggagcattg tctccgtcaa agttgtattt tgacagtagg
      481 tcgcgcactt ccagttcgac aagatccaac atttcgggat catccaccat gtcacacttg
      541 ttcaggaaca caacgatttg gggaacgttc acctggcggg caagcagaac gtgctcgttg
      601 gtttggggca tgggaccgtc tgtggcagct acaaccagga tagcaccgtc catctgggca
      661 gcaccggtaa ccatgttctt cacgtagtcg gcgtgtcccg ggcagtcaac gtgtgcgtag
      721 tgacgcgaag ctgtctggta ttcaacgtga gatgtattga tggtgatacc gcgttctttt
      781 tcttcaggag cgttatcaat agaatcgaat gaacgtattt cagacaggcc cttttttgcc
      841 agtacctggg taatggcggc tgtcagcgtg gtttttccat ggtcaacgtg accaatggtt
      901 ccaatgttta cgtggggttt atcccttttg taggtttctt ttgccataac ttaataattt
      961 aataactcaa taatgtaaat ttgagccggt gatgagattt gaactcatga cctcttcctt
     1021 accaaggaag cgctctaccc ctgagctaca ctggcctctt ttttgcctgt tggagcggaa
     1081 gacgaggttc gaactcgcga ccctcagctt ggaaggctga tgctctgcca actgagctac
     1141 ttccgcatgg tgtgggaggg gcaggattcg aacctacgta gacgtgcgtc agcagattta
     1201 cagtctgccc cagttggcca ctttggtacc ctccctcaat aattcatttt gtcaaacaac
     1261 aaccgagccg atagagggat tcgaacccac gacctgctga ttacaaatca gctgctctga
     1321 ccaactgagc tacatcggca aaaacatccc ggcattttgg agcggcaaag ataaaccatt
     1381 tctacaaact accaaaaaaa aacgataaaa aactcccgct attacaatga ttcctaagaa
     1441 ctacaactta tccggctaag gcaataattg cctgaaggat gctctctgta tcgtacccgc
     1501 attcatggta cagttgcttt gttttaccat gctggataaa acggtccggg atcccgagcc
     1561 ctgtgacttt gcttatggcc tctttatgtc ttgccagata agtggatacc gtactgtgta
     1621 gtcctccaat cagacatccg tcctccaagg taatgacttt actgaaatta cgggacacat
     1681 attccaggac ttcaccatca atgggtttga ggaaacgcat atcgtaatgt tgaaccgaaa
     1741 tccctttttc agcagctttt gccacagcgg cggctcccat gataccggca gcccccaggc
     1801 tcagcagcgc catgtctttt ccctgggaca gcagccgtgc cctgcctggt tccaggtatt
     1861 ggaaaggtct tctccaatcc ttaattccgg cgcctgtccc tctggggtag cggatactgg
     1921 tagctgtata ccttttgtcc tgtgccgagt acatcatatt cctcagctcc acctcgtcca
     1981 tgggagcggc cacaatcagc ccgggaacac aatttaaata cgccagatcg tatgccccgt
     2041 gatgggtcac tccgtcttcg ccaaccagtc ctccccggtc aagacagaaa accaccttca
     2101 ggttctgtat ggccacatcg tggatcaccg aatcgtatgc cctttgcatg aagctggaat
     2161 agatgttgca ataggggagg aagcctgcag cggcaaggcc ggcagaaaag gtgacagcat
     2221 gttgctcggc aatgccaacg tcaaaacacc tgtgtggcat ttcttccatc ataaggttca
     2281 tagaacagcc gcttggcatg gccggcgtga tacctgtaat gtcgggattg gtgcgggcta
     2341 actccagtaa ggtttggcca aagacatcct gaaacctcgt ctttgtagga acatcaggat
     2401 catcatcggt gagtctgtgt cctgtttccg catcaaaaag tcccggagca tgccaggtgg
     2461 gctggtcatt ctctgccggg gcatatcctt ttccctttat tgtaagagca tgcagcagcc
     2521 gtggcccctt caggtttttt agccttttga aagtattaac cagaaggatg acatcgtgcc
     2581 cgtcaaccgg gccaaaatac cggatgccaa gtggctgaaa caatgtactg ttcttgacaa
     2641 agagacgttt tcctgctttt accgtctctt gaattgcatc gcggatggtt cccgaaccca
     2701 acgctttcca cgtcttcatt tttagcctgt tatacttgcc cgacgtagtt agtttcaaaa
     2761 gagaattgtg cagcccgccc acatttggtt caatagacat cttgttatca ttgagcacaa
     2821 taagcatatc tgcgttacat cctccggcat tgttcaaacc ttcgtacgcc agtccggcag
     2881 tcatggaacc atccccaata acggccaccg ttttttcatc acttttacgc atggcagcgg
     2941 ctgtggcata tcccagagct gccgatatgg atgtggaaga gtgcccggtt ccgaaggcgt
     3001 cgtaaggact ttcatccatt ttggggaagc cgctcaaacc gttgtaacag cgattcctca
     3061 ggaactcttt gcgacgtcct gtaaggatct tgtgcgtata agcctggtgg cccacatccc
     3121 aaatgatctt gtctttagga gcatcgtata cataatggag ggccacagtc agttctaccg
     3181 ttcccaggct ggctcccagg tgaccaggat tatgtgagca gcattcggta atgtatttcc
     3241 ggatttctgc gcacagttga ggcaattgtt ccggttttaa ctttttcagg tcttccgggc
     3301 tgtcaatttg ttctatcaga ttatccatga tatgatcaat ccctgataat tgtctgttcc
     3361 ctgtcgggtc ccagggaaat gatttttacg ggaacgcctg tggcatcttc gatgaaccgc
     3421 acataagtca tcagttcata aggcaattct tcttctttac ggagggcaga cagtttttta
     3481 ccccagcccc tgaattctct gtataccggc gtgataggtg tgtgtgtgtc aaatgggaca
     3541 cggttgattt cctgtccctg gaccgtatag gaggtggcaa tttttatggt gtcaaatgcg
     3601 tccagaacat cggctttcat catgatcaga tctgtcaccc cattgaggat cacagcgtat
     3661 ttcaaggcca ccagatccag ccatcccgtt cgccggggcc ttccggttgt agcaccaaat
     3721 tctttaccct gctcccggat tatttcgcct gtccggtcat tgagttcagt tggaaaaggt
     3781 ccgcttccca cacgggtaca gtaagctttg aatattccat acaccctgcc gaccctggtg
     3841 ggaggaaatc ccaggcctat acaggctccg gcacaggtag tagaagatga ggtaacaaag
     3901 ggatacgatc caaaatcaat atccagaagg gtcccctgag ctccttctgc caggacccgt
     3961 ttgttttcct tcaggcaatt agataagtaa tactctgcat ctatcagggg aaactgtttc
     4021 atggtttcaa tggcgttgag ccaattatct tctccctggt ctgcgtcata cgcatagtcg
     4081 aattgcctga gaaaggcaat atgctttgat ttcagtgcca ggaagcggtc ccggaaatca
     4141 ggtgcaataa tatcccccac gcgtatcccg ttccggcccg tcttatccat ataggctggg
     4201 ccgatgcctt tcagggtaga accaatccgg gaggcaccct tggaatgctc actggctgca
     4261 tctataatac ggtgagtggg aaggatcagg tgtgcccttt tcgagatcat cagacgatcc
     4321 ctgaccggaa tccccagtgc ttccagggaa cggcattctt ccccaaatat cacgggatca
     4381 atcacaacac cgttacctat tatattcatg gtattgctgc gaaagatccc ggcaggcaca
     4441 gaatgaagaa caaaggagga gccttcaaaa tgaagggagt gcccggcatt gggtcctccc
     4501 tggaaacggg cgattaccgg atatcgttgt gccagataat ctacaatctt gcctttgcct
     4561 tcatcacccc actggagacc taaaactata tctatattca taataatttt ttcaccacag
     4621 ccttcaaagg tacgtaaata tttgtacctt agcaaatctt taagaaatac aattaacctt
     4681 ctttcttatg actcaagtta aaaaattact gaacgacagt tttgtggcac gctgggcggc
     4741 attgttgctc attgccttaa tgatgttttt tgcctatatg tttgtggatg tgatgtctcc
     4801 gctgcagaca ctggtggaag gccaacgggg gtggacccct ggcatttttg gaacgtatgc
     4861 agcttctgaa tattttctga atgtgtgcgg ttttcttatt cttgccggaa ttattctgga
     4921 taaaagcggt atccgtttta cgggggcatt gtccgcttcc cttatggtga taggagcatc
     4981 tatcaaatat ataggaatca gtgattggtt tcaggaaact gagttgtgtg cctggctaaa
     5041 tacctggtgg gtttctcttc cgggcagtgc caaaatagct tctttaggat ttatgatttt
     5101 tggttgcggt tgtgaaatgg cggggatcac tgtttccaaa gccattgcca aatggttcaa
     5161 aggcaaagaa atggcgctgg ctatgggtct ggaaatggct atagcccgtt tgggcgtgtt
     5221 tactgtgctt tctctgtctc catttattgc caacaagttt aacggatcgg tggtggctcc
     5281 cattgctttc tgtacagtgc tgttgctcat cggccttatc aatttcctgg tgtttaacgt
     5341 gatggatgcc aagctggatc gtcagatggg tgatgcccga gggaatgaag ccccggcgga
     5401 agaatttaaa gtcagtgaca ttaaaaatat ttttggtagc aagatgttct ggattattgc
     5461 cttgttgtgc gtgctatatt actcggccat tttcccgttt cagaggtacg ccactaactt
     5521 cctggaatgc acactctcca taccaacgga aagcgcagcc aatctgtttc gctggttccc
     5581 catattggca atggtgctga ccccgttcct gggatcgttt attgatcacg tgggtaaagg
     5641 cgcttcaatg cttatgtacg gggcgttgat tatgattatc tgccacctga cctttgcgtt
     5701 tgttttgcct ctgtatccca aggaatggtt tgcactgctt attattgtag tacttggagt
     5761 ttctttctca ctggttcccg cctctttgtg gcccagtgtt ccaaagatta tcgacgaacg
     5821 ttatttgggc agtgcctatt cactcatctt ctggattcaa aactttgggc tttgctttgt
     5881 acccaagatt attggcaatg tgttggacag caccaatccg ggagtggccc agttgcgtga
     5941 acaggccaat gcccagctgg cacaaggcat acaggctgtg gtgcctgcct atgattacac
     6001 tgtgccaatg gtcatttttg cttcgttcgg agtgcttgcc tttatcctgg gcttatggct
     6061 caaggccgag gaccgcgcga agggttacgg tctggaatta cctaatatca ccaataaagt
     6121 caagaaataa tgaataaagt tgtcaagttg ctcagggatt ccaggtgggt gcgctgggcc
     6181 gttcttttat gcctttcgct ggtcatgttt gcctcctact tctttgatga ccaattcacc
     6241 accatcggcg acatattcaa aaaccctgct attcttgatc tgtcctggac ttcgggtgat
     6301 tatggcatgt acgggggagc ctactcgctc ctctgtgtgt ggggaggttt gataatatgc
     6361 ggtatgttgc ttgataaatg gggggtgcgt gtaacaggga cgttgtttgt gctactgatg
     6421 gtagggggag cagccattgt ggtgtatggc atttcggctt ctttcaacga cagcggattg
     6481 taccatgtga tggcacgggc ctttgacaaa ccctcactgg ccatggctta tgcgggatgt
     6541 gccgttttcg gactgggaag tgaaattgca ggagttgctg ttacacgttc catagcgaaa
     6601 tggttccgtg gaaaggaaat ggccctggcc atggggttac agctcgccct ggcccggctg
     6661 ggaacggcta cggccctgat tgttgttccc cgcattgtta acctgaacga tacccttata
     6721 cctttttcag aaacgagcaa gccggcactg gcaggattca tactcctgct ttgcggcatg
     6781 gtggtgtggg gagttttcct ggccttcgat aagaaactgg acacccagac agcagcagaa
     6841 gccctgaaaa attcacaacc ggtaagtaag gaagataagt tcaaattttc ggatatattc
     6901 aagatacttg gcaacaagca ttacattctc atttccctgt tgtgcgtgtt tttctatgcc
     6961 tgtatcattt ctttccgtcg atttgccact tccatcatca taccgcgttt tgaaattgaa
     7021 gacaatatag cctccctgat ggtggcgctg atacctttca ccaccatgat tttcaccccg
     7081 attttcggat ccctggtaga tttccgtggc aaagccacca ggtggatgat actgggttcc
     7141 tgtctggtac tggtggcaca tttgatcatt ggatttgctc ccggactccc tgttttcgga
     7201 tacttaggca tagccatact gggcatcggg tattcattgg taccggctgc catgtggccc
     7261 tccgtgccca agataatccc ggaaaagaac ctgggaacgg cctactcgct catctactgg
     7321 atccagaata tgggcatgtt gctggttccc atctttgtag ggcagatcat agaaaagaac
     7381 ataaacgacc agatccgtgc tgcggtacag gcagaatacc ttttcatagg cctggccctg
     7441 gtagccattg ccgtatcgct aatattaagc cgttctgcag ataaaaatcc gcagctgcag
     7501 ctggatgatc ccaataaggt ccgcaaaacg gtcaactaga taacgcctcg ggctcatttt
     7561 gagccgcagc gccactaccg gtcagacata agggtgatca gaaaggcaga tcgtctacgt
     7621 ccggct
//
LOCUS       DAOSOF010000094         7581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9688_length_7581_cov_5.372575, whole genome shotgun sequence.
ACCESSION   DAOSOF010000094 DAOSOF010000000
VERSION     DAOSOF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9688_length_7581_cov_5.372575"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLM68_06170"
     CDS             complement(<1..1229)
                     /locus_tag="PLM68_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT54980.1"
                     /translation="MMKRLLMIVMILALAPATSQAQSILRRLERTARSAAERTVERKV
                     EEKVEEGVNKAIDKAFEEKETEESASSQASGATSRQASDTASPQDPAPASPQNKRAEI
                     AWSKSDFVPGDEIFFEDNLTGEKMGEFPSQWDILQGNAEVAQIDGANAILLSDDANLA
                     PLMENPKAFLPEIFTLEFDFFLNEMTEENSDSGFSRYHVLFYDNKSGEYEDTDNNTME
                     LDWYPPYGEIECHYSTASYEDRSASAQLELNFGEWNHLAISFNKRALKIYINGIRIIN
                     LPNVAAPMRFSIGQGWSQPNTAFIRNVRLAKGAVPLYDRMMQDEKFISYGITFDVSKS
                     VIKPESAGELTRIVQLLKENPSLRFSVKGHTDATGSASANQTLSEARAEAVVARLVEM
                     GIDRSRLSAKGLGQNSP"
     gene            1448..3628
                     /locus_tag="PLM68_06175"
     CDS             1448..3628
                     /locus_tag="PLM68_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT54981.1"
                     /translation="MKKILIITGICMLAACSADTEKQIQDLTAQMTLEEKVDMLHGQT
                     MFTSAGVERLGIAPVKYADGPFGIREELEPHSWRPLGLTTDSATFFPTGSALAATWSE
                     EMAYLYGKGMAEEARTRGKDMILGPAINIQRIPTGGRTYEYFSEDPLLSSRLAVGYVR
                     GVQDNGAAACIKHYAMNNQENNRGMVDVIVSDRAMREIYLPPFRAAVEEAGALGVMSA
                     YNKVNGTWCAENEILLDKILRQEWGFRGMVISDWYGTHSTVQAALAGLDVEMPGNMFF
                     GQALLDSVRNGAIPEEIIDRKVGNILRVRLAIDPVEETSHVNPVSTPEHGQMVYKIAS
                     RSIVLLKNEGSLLPLDLEKTPTIAVIGDNAIQKHATGGIGAGVKTRYEITPLEALEQK
                     IGDRATLVYARGYKGFSRQDRERGGILGGVVLSGAGGMLTGSAAPGTGLDQEMLAQAL
                     EAAAGADMVLFFAGNNREVETEGADRMNIILPSLQDQLIREIATVNPNIVTVVVTGAP
                     VDLSTVEPCSKAMLVSWFNGSEAGNALADVLLGTVVPSGKLPFTFPVRLEDSPAYALK
                     TYPNFDKAPYSEDIYVGYRWFDAWKIEPLFPFGHGLSYTTFTYDSPRTGKETYRTSDV
                     MTVSFRLSNTGKYPAREVVQLYVHRPGSNVEFPENELKAFKDVQLEPGKSAQVTLKVP
                     ASSLMYWNTSKGNWTLEPGTVELRIGSSSRDIRLKKSITLRGSN"
     gene            3631..4710
                     /gene="carA"
                     /locus_tag="PLM68_06180"
     CDS             3631..4710
                     /gene="carA"
                     /locus_tag="PLM68_06180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOT54982.1"
                     /translation="MMNKARIILEDGSVFEGRSFGCKKSVSGEVVFSTGMTGYPESLT
                     DPSYLGQILVMTYPLVGNYGVPERTVDKQGLCLYMESEKIHVQGLVVFDHSRLFSHWN
                     ARESLEQWLEEEQIPGVYDVDTRALAKLLREKGSMKGKIVVGEDDPLSIPFPDNTANN
                     TAESISCREVITYQGNPGIKKVVLIDCGVKHNIIRILLSRGVTVIRVPWDHDFISMDY
                     DGIFISNGPGDPTRYTATIAHLKKAMEGNKPICGVCMGNQVLGLAAGAQVIRMKYGHR
                     GHNQPVQDMDTGRCYITSQNHGYALDPDTLGKDWGTMFINLNDGTNEGIRHRTKPFFS
                     LQFHPEAASGPTDMVPVFDQFTEIL"
     gene            4715..>7581
                     /gene="carB"
                     /locus_tag="PLM68_06185"
     CDS             4715..>7581
                     /gene="carB"
                     /locus_tag="PLM68_06185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOT54983.1"
                     /translation="MKFEKILVLGSGALKIGEAGEFDYSGSQALKAIREEGIRTVLIN
                     PNIATVQTSEGVADTVYFLPVTPFFVEQVIRKEKPGGILLAFGGQTALNCGVELYRNG
                     VFEKYGVQVLGTPIQAIMETEDRELFVRKLNEIDIKTIQSVAVGAVEEAAHAARNLGY
                     PVILRAAYALGGLGSGFCDNEEELLALAEKSFAFSPQVLVEKSLKGWKEIEYEVVRDK
                     YDNCITVCNMENFDPLGIHTGESIVVAPTQTLNNQESPLLREMAIKIIRHIGIVGECN
                     IQYALDVASEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLALGYGLHELKNSVTR
                     VTPAFFEPALDYVVCKIPRWDLGKFRGIAREIGSSMKSVGEVMAIGRTFEETIQKGLR
                     MIGQGMHGFVENRELVIEDIDKALHEPTDMRIFVISNAMQAGYTVEQIHGLTRIDRWF
                     LQKLMDIVQTSKLLEKTNPPKDPDGGDWGEQERELLYIAKRQGFSDFQIARALWKKRM
                     TQDSPLQIRRLRKALGITPVVKQIDTLAAEYPARTNYLYLTYGGREHDIDYEQDGRSI
                     VVLGSGAYRIGSSVEFDWCGVQALLTIRREGWRSVMINYNPETVSTDYDICDRLYFDE
                     LTNERVMDIVELETPKGVILSTGGQIPNNLAVVLDKQQVPILGTPARYIDNAEDRHKF
                     SAMLDRIGVDQPRWKELSSMRDVTAFVREVGFPVLVRPSYVLSGAAMNVCSNFEEMER
                     FLKLAANVSKQHPVVVSQFIEHAKEVEMDAVARDGDIIAYAISEHVEFAGVHSGDATI
                     QFPPQKLYVETVRRIKRISRQIARELHISGPFNIQYLAKGNEIKVIECNLRASRSFPF
                     VSKVSKINFIEMATRIMLGLPVERPRKNEFDLDYVGIKASQFSFSRLQKADPVLGVDM
                     ASTGEVGCIAEDTLEAVLISMLAVGYRVPARNILLSTGTPR"
BASE COUNT         1879 a         1868 c         2113 g         1721 t
ORIGIN      
        1 atggggctgt tttggccgag gcctttggcc gaaaggcgac tacggtcaat gcccatttcc
       61 accaggcggg caacaacggc ctcggcccgg gcctcggaaa gtgtctgatt agcagaagcc
      121 gatccggtgg cgtcggtgtg gcctttcacg ctgaagcgaa gggacgggtt ttccttcaac
      181 agttgtacaa tgcgagtcag ttctccggcc gattcgggct tgatgacgct tttggacaca
      241 tcaaaggtga tcccgtaact gatgaatttt tcgtcctgca tcatgcggtc gtacagggga
      301 acggctcctt tggccagacg tacattgcgg atgaacgcgg tattaggctg cgaccagccc
      361 tgtcctatgg aaaagcgcat tggagcggcg acattgggga gatttattat gcggattccg
      421 ttgatgtaga tcttgagcgc tcttttattg aatgaaatgg caagatggtt ccattccccg
      481 aaatttaatt caagttgggc cgaagcactc ctgtcttcat acgaggcagt actgtaatgg
      541 cattcaattt ctccataagg aggataccag tccagttcca ttgtgttgtt atccgtgtct
      601 tcatattcac cggacttgtt gtcataaaac aacacatggt atctgctgaa tcctgaatca
      661 gaattttctt ctgtcatctc attcaggaag aaatcaaatt ccaaagtaaa aatttccggc
      721 agaaaagcct tcggattttc catcaaaggt gccagattgg catcgtcaga tagcagaatg
      781 gcgttggccc cgtcaatttg agccacttct gcattgccct gcagaatatc ccactgggaa
      841 ggaaactctc ccattttttc gcctgtcagg ttgtcttcaa aaaagatttc gtcacccggc
      901 acaaagtcag atttggacca tgcaatctcg gcccgcttgt tttggggact tgccggggcc
      961 gggtcctggg gggaggctgt gtcggaggct tgccgggatg ttgccccgga ggcctgactg
     1021 gaagcggact cttcagtctc tttctcttca aaagccttgt caatggcttt gttaacacct
     1081 tcttccactt tttcttccac tttgcgctcc acggtccgtt cagcagcact gcgggctgtt
     1141 ctttcaagcc tgcggaggat ggattgcgcc tggcttgttg caggtgccag ggccaggatc
     1201 attacgatca tcagtaagcg tttcatcatc agtatgggtt tctatattag taattgagaa
     1261 cttgttatat tgcctttatc gttgaagggt tcctggcgta caccgcccat gccccttgct
     1321 tgcccttgcc tggtggtgga aacagttttt tgcataaaca cctggctttg ggtaaccatt
     1381 tccacagggt caaaagatga atatacgaag ataaacattt gtttttttga atgattatat
     1441 ttgtatcatg aaaaaaattc taataataac gggcatttgc atgctggctg cctgcagtgc
     1501 cgatactgaa aaacaaatac aggatctgac agcgcaaatg actcttgaag aaaaagtgga
     1561 catgctgcat ggacagacca tgttcacttc tgccggtgtg gaacgcctgg gcatagctcc
     1621 cgtcaaatat gcagacggac cattcggcat tcgtgaggaa ttggaacctc attcctggag
     1681 gccgttgggc ctgaccacag actcggcaac attctttcca actggttctg ccctggccgc
     1741 tacctggagc gaagagatgg cataccttta cggaaaaggg atggcagaag aggccaggac
     1801 ccggggcaaa gacatgattc tggggccggc catcaatatc cagcgcatac ccacgggcgg
     1861 aaggacatat gagtacttca gcgaagaccc gttactaagt tcacgcctgg ccgttggtta
     1921 tgtgaggggt gtgcaggaca acggggcagc tgcctgtatc aaacattatg ccatgaacaa
     1981 ccaggaaaac aaccggggca tggtggatgt gattgtcagc gacagggcca tgcgcgagat
     2041 atacctgcct cctttccgcg ctgccgttga agaggcggga gcactggggg tcatgtccgc
     2101 ttacaacaag gtgaacggga cctggtgtgc cgagaatgag atcctgctgg acaaaatatt
     2161 gcggcaggaa tggggtttcc ggggtatggt catctctgac tggtacggca cacacagtac
     2221 cgtacaggca gccctggcgg gactggatgt ggaaatgccc ggaaacatgt tttttggaca
     2281 agccctgctg gattcagtac gaaacggtgc aattcccgaa gagatcattg accggaaagt
     2341 gggcaacatc cttcgggtgc gccttgccat tgacccggtt gaggaaacat cccatgtaaa
     2401 tcccgtcagc acacccgaac acgggcaaat ggtctataag atcgcttccc ggtccatagt
     2461 cctgttaaaa aatgaaggtt ccctgctacc gctggacctg gaaaagacac caaccatagc
     2521 agtcataggg gacaatgcca ttcaaaaaca tgccaccggc gggatcggtg ccggggtgaa
     2581 gacccgctac gagattaccc cgctggaagc cctggagcag aaaatcggtg atcgcgcgac
     2641 attggtgtat gcccgtgggt acaagggatt ttccaggcaa gaccgggaac gcggaggcat
     2701 attgggcggt gtggtgctct ccggtgcagg aggcatgctc acgggcagtg ctgccccggg
     2761 aacaggcctg gaccaggaaa tgcttgccca ggccctggaa gcagcagccg gtgccgatat
     2821 ggtcctgttt ttcgcaggta acaaccggga ggtggagacc gagggagccg acaggatgaa
     2881 catcatcctg ccttccctgc aggaccagct gatccgggaa attgccacag tcaatcctaa
     2941 catagtgacc gtagtggtaa ccggggcccc ggtggacctg agcaccgttg agccgtgttc
     3001 gaaggccatg cttgtatcct ggttcaacgg ttccgaagca ggcaacgctc tggccgatgt
     3061 gttgctgggt actgtagtgc cttccgggaa gctgccgttc acattccccg tacggctgga
     3121 agattccccg gcctacgcat tgaagactta tccgaatttt gacaaggccc cttactcgga
     3181 agatatttat gtgggttacc gctggttcga tgcctggaag atcgaacccc tgtttccatt
     3241 tgggcacggc ctgtcctata caacttttac ttatgattct ccccggaccg gaaaggaaac
     3301 ataccgcacc tccgatgtga tgacggtctc cttccggcta tccaataccg ggaaataccc
     3361 tgcccgcgag gtggtgcaac tgtatgtgca ccgtcccgga tccaacgtgg aattcccgga
     3421 aaacgaactg aaagctttta aggacgtaca gctggaaccc ggaaaatccg cgcaggtcac
     3481 cctcaaggtg cccgcaagca gtttgatgta ctggaatacg tctaaaggaa actggacact
     3541 ggaaccgggt acggtggaac tgcgtatcgg ttcctcttcg cgtgatataa gactcaaaaa
     3601 aagcataact ttgcgcggtt ctaactaagc atgatgaata aagctcgtat tatcctggaa
     3661 gacggatctg tttttgaagg ccgctccttc ggctgtaaaa aatccgtttc gggagaagtg
     3721 gtgttcagta caggcatgac gggatacccc gaatctttga ccgatccttc ctacctggga
     3781 caaatccttg tcatgactta ccccctggtg ggcaattacg gtgtgccgga aaggacggtg
     3841 gacaagcagg gactttgcct ctatatggaa agtgaaaaga tccacgtgca gggactggtt
     3901 gttttcgacc acagtcgttt gttcagccac tggaatgcca gggaaagcct tgaacaatgg
     3961 cttgaagagg agcaaattcc cggtgtgtat gatgtggata cgcgtgcgct tgcaaaatta
     4021 ctgcgggaaa agggctctat gaagggaaaa attgttgtgg gggaagacga tccgctgtcc
     4081 attccctttc cggacaacac ggcgaataat acggcagaga gcatatcatg cagggaagtg
     4141 atcacctacc agggaaatcc gggaatcaaa aaagtggtgt tgatagattg tggcgtgaag
     4201 cacaacatca tacgcatatt gctcagccgc ggtgtcactg tgataagggt cccctgggac
     4261 catgatttca tatctatgga ttatgacggg atctttatca gcaacgggcc aggcgatccc
     4321 accaggtaca cagcgaccat tgctcatctg aagaaggcta tggagggaaa caagcccatc
     4381 tgtggcgttt gcatggggaa ccaggtactg ggactggctg ccggggcaca agtgatcaga
     4441 atgaaatacg gtcacagggg tcataaccag ccggtacagg acatggatac cggaaggtgt
     4501 tacatcacct cccagaatca cggatatgcc ctggatccag acaccctggg caaggattgg
     4561 ggcacgatgt tcattaacct gaacgacggc acaaacgaag gaatccgtca caggaccaaa
     4621 ccgttctttt ccctgcaatt ccatcccgaa gcagccagcg gaccaaccga tatggtcccc
     4681 gtttttgacc aattcactga aatcctgtaa accgatgaaa tttgaaaaaa tattggttct
     4741 cgggtccggc gccctcaaaa tcggcgaagc cggagaattt gactattctg gctcccaggc
     4801 cctgaaagcc atccgcgaag aaggaatccg tacggtcctg atcaatccca atattgccac
     4861 ggtgcagaca tccgaaggtg tggccgacac ggtatatttt ttacccgtca cccctttttt
     4921 tgtagaacag gtcatcagaa aagaaaagcc cggtggcata ctgctggctt tcggaggtca
     4981 gactgccctg aattgcgggg tggaacttta tcgtaacggt gtctttgaaa aatacggggt
     5041 gcaggtgctg ggaacaccca tacaggccat catggagaca gaggacaggg agctttttgt
     5101 caggaaactg aatgaaatag acatcaaaac cattcagagc gtggcagtcg gcgcggtgga
     5161 agaagcggca cacgccgccc gtaacctggg gtatcctgtc atcctgaggg ctgcgtatgc
     5221 cttgggcgga ctcggctcgg gtttttgtga caatgaagaa gaactgctgg cgctggcaga
     5281 gaagtctttt gccttttccc ctcaggtgct ggttgaaaaa agcctcaaag ggtggaaaga
     5341 gattgagtat gaagtggtac gggacaagta cgacaattgt atcaccgtat gcaatatgga
     5401 gaactttgat ccgcttggca tccatacggg agaaagcatt gtggtggcac ccacccagac
     5461 actcaataac caggaatcac cgctgctgag ggaaatggcc atcaaaatca tacggcatat
     5521 cgggattgtg ggggaatgca acatccagta tgccctggat gtggccagtg aagattaccg
     5581 cgttatcgag gtcaatgccc gtttaagccg ttcatcggca ctggcatcaa aggcaaccgg
     5641 atatccgctg gcttttgtgg ctgccaagct ggccctggga tacgggctgc acgagctgaa
     5701 aaattccgtt accagggtca ctccggcttt ttttgaaccg gcactggatt atgtggtctg
     5761 caagatccca cgctgggacc ttggcaagtt ccggggtatt gcccgggaga ttggcagcag
     5821 catgaaatct gtcggggaag tgatggcgat cggacgcact tttgaagaaa ccatacaaaa
     5881 gggattgcgc atgataggcc agggaatgca cggtttcgtt gagaaccggg aactggttat
     5941 agaggatatc gacaaagcgc tgcacgagcc taccgacatg cggatctttg tgattagcaa
     6001 tgccatgcag gccggctaca cggtggaaca gatccacgga ctgacccgga tcgacaggtg
     6061 gttccttcaa aaactcatgg acattgtcca gaccagcaaa ttgctggaaa aaacaaatcc
     6121 cccgaaagat cccgatggcg gcgactgggg cgaacaggag cgagagttgt tgtatatagc
     6181 caaaagacag ggcttttctg acttccagat agcaagggcc ttgtggaaaa agaggatgac
     6241 ccaggatagt cccctgcaga tccgccggtt aaggaaagcg ctgggtatca ctcctgttgt
     6301 caagcagata gataccctgg cagccgaata tcctgccagg accaattacc tgtacctgac
     6361 ctacgggggc agggaacacg acattgatta tgagcaggac ggacgctcca tagtagtcct
     6421 gggctctggg gcttaccgca ttggaagctc agtggaattc gactggtgcg gggtacaggc
     6481 cctgctgacg atccgcaggg aaggctggcg ttccgtaatg atcaattata atcccgaaac
     6541 ggtttccacc gattacgaca tatgcgacag gctctatttt gacgagctca ccaatgaacg
     6601 ggtcatggat attgtggaac ttgaaacacc caaaggggtg atactttcca ccgggggaca
     6661 gatccccaac aacctggccg tggtgcttga caagcagcag gtgcccatcc tgggaacgcc
     6721 tgcccgctat atcgacaatg ccgaggacag gcataaattc tcggccatgc tggaccgcat
     6781 tggggtagat cagccccgct ggaaagaact ctcttccatg agggatgtca ccgcttttgt
     6841 ccgggaggtg ggattcccgg tacttgtcag accgagttat gtgctgagcg gtgcggccat
     6901 gaacgtttgc tccaatttcg aggagatgga gcgcttttta aagctggcgg ccaacgtgag
     6961 caagcagcac ccggttgtgg taagccaatt cattgaacat gccaaggaag ttgaaatgga
     7021 tgccgttgcc cgtgatggtg atatcattgc ctacgccata agcgaacacg tggaatttgc
     7081 cggtgtgcac agcggcgatg ccaccatcca gttcccgcct cagaagttgt atgtggaaac
     7141 ggtcaggcgc atcaaaagga taagccggca gatagcccgt gaactgcaca tatcgggtcc
     7201 tttcaacatc cagtacctgg ccaagggcaa tgagatcaag gtgattgaat gcaacctgag
     7261 ggcatcgcgc agcttcccct ttgtcagcaa agtgagcaag atcaatttca ttgagatggc
     7321 aacccggata atgctgggac ttccggtgga gcgcccccgc aagaacgagt tcgacctgga
     7381 ttatgtaggg ataaaggctt cccagttttc attcagccga ctgcaaaagg ccgatcctgt
     7441 actgggcgtg gacatggctt ccaccggcga ggtgggatgc attgcagaag acaccctgga
     7501 ggctgtcctg ataagcatgc tggccgtggg ttacagggtc cctgcccgga acatcctgct
     7561 ttccacggga acaccccgcc a
//
LOCUS       DAOSOF010000095         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9711_length_7565_cov_4.229827, whole genome shotgun sequence.
ACCESSION   DAOSOF010000095 DAOSOF010000000
VERSION     DAOSOF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9711_length_7565_cov_4.229827"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLM68_06190"
     CDS             complement(<1..834)
                     /locus_tag="PLM68_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT54984.1"
                     /translation="MSKKLKLEISDNMYQKALKIIPGAIAGIRRPYNFVPGEYPIYFD
                     HAKGGRVTDVDGNEYIDMLCAYGPIIIGHREEEIDNAVIEQIQNKGFCFSLTQPIQNK
                     LAEKLIELIPSAEMASFVKTGSDATSVAIRVARASTERVKVIRCGYHGWHDWCVEVKG
                     GIPRKLWEDVYEFHYNELDELEDLMKQHGDDTAAIIITPVGHPLAEKVQMPKPGFLEG
                     VRALADKYGTVLVFDEIRSGFRYDLGGAQKVFGVTPDLSAFGKAMANGYPIGAVVGKE
                     KH"
     gene            complement(859..1506)
                     /locus_tag="PLM68_06195"
     CDS             complement(859..1506)
                     /locus_tag="PLM68_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOT54985.1"
                     /translation="MSKQISLQEAVSFIKSGMTIMVGGFLGLGSPQRILDALLETDVK
                     DLTIITNDTGFPDKGPGKLIARHRVSKVITSHIGTNPATGEQYHSGELQVIFVPQGTL
                     IEQIRCAGAGLGGILTPTGLGTIVAEGKDIINVDGKDYLLEKPLKADIALIGASMADE
                     EGNLYFRGTSRNFNVMMAMAADLVIADPKEIVKTGDILPENVHTQSIFVNYIVGK"
     gene            1603..3096
                     /locus_tag="PLM68_06200"
     CDS             1603..3096
                     /locus_tag="PLM68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOT54986.1"
                     /translation="MIITVENFLLIGSLLLFFSLLAGKTGYRFGVPTLLLFLVVGMIF
                     GSEGLGLQFSNPKTAQFIGVVALSIILFSGGMDTKYQEIKPIARQGIILATFGVLLTA
                     ILTSLFIYGLTLLIPGFNLSFPVCLLLGSIMSSTDSASVFAILRSKGLKLKHNLRPLL
                     ELESGSNDPMAYMLTITMIQVIMTTEGLRIWQVVLLFFYQMITGGLAGYLLGKLCAYL
                     MRKFNIGNDSLYPILILAFCFFIFAFADLIKGNGFLAVYIGGLVIGNSRFLQKRNVMN
                     FFDGLSWLSQIIMFLALGLLVNPSELWPIAGVGLLIGIFMIVLARPIAVLLSLFPFRM
                     MTTNSRIFTSWVGLRGAVPIIFATYPLVSGIAEASILFNIVFFITILSLLVQGTSIPW
                     IAKKLHVSIPQKIARRSEFEMEFSEDIKSAMSEVVVTQESLRFGSRMTDIPLPDKTLV
                     VMVKRGDSFFIPRGGTEIHIGDKLLIISDDEKALRETYGKLGMDSDI"
     gene            complement(3147..4394)
                     /locus_tag="PLM68_06205"
     CDS             complement(3147..4394)
                     /locus_tag="PLM68_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOT54987.1"
                     /translation="MTTFHIPIPGVRIKDYSYELPDNKIAKYPLPVRHDSKLLLYRDG
                     RVSDHVFVDLPSLLPQESLLVFNNSKVVPARLFFRKETGAVIEVFCLEPASPREYILA
                     FSQHTSCTWYCAVGNAKKWKTGNLPLYNPQNDPKLSALGLSASLIGQEADRYLVRFSW
                     TANMPFSSLLEATGRVPLPPYLKRESEALDLERYQTIYARKRGSVAAPTAGLHFSEKV
                     LSDLQKRGMGIAELTLHVGSGTFRPVKTPYIRDHIMHSEPFSVTREFLEQLTAHRGPV
                     VSVGTTSCRCLESLYYLGLQAAGGKTPSFVGQWEPYVTSSGMPYRESLGYLRAYMDRV
                     QKDILDASTQIIIAPSYRFLTTDILITNYHQPNSTLLLLVAALTGEDWRVIYDHALAG
                     NYRFLSYGDSSVLFNPEHRISNK"
     gene            4833..5300
                     /locus_tag="PLM68_06210"
     CDS             4833..5300
                     /locus_tag="PLM68_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT54988.1"
                     /translation="MFTNVFSIIVTIVNYICITFVKTKFTIMRTRLQQFLTAENISPS
                     RFADQIGIQRSGLSHILAGRNKPGFDFIEKMLLTYPSLNAEWLITGKGKMYRETREKE
                     PELLPEEPPIQETKNLFADTYNILPEAGHKLIKVLLFYDDNTFTEHNPADASL"
     gene            5353..6375
                     /locus_tag="PLM68_06215"
     CDS             5353..6375
                     /locus_tag="PLM68_06215"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOT54989.1"
                     /translation="MYRIIRPLLFLFPPETAHNLAFKALAIVKYIPLGTRILRSLFSY
                     NSPSLQRQVMGIDFPNPVGLAAGLDKKAEVYNEMGALGFGFVEIGSVTVAPQEGNPKP
                     RSFRLVKDRALINRMGINNPGVKQIVRNIQQNKPRVIIGGNITKNTSTPNDQAYKDLE
                     KAFAQMYDYVDYFVVNVSCPNVKDLRSLQTVDNLTGITRHLTEIRRYSDVYRPILLKV
                     SPDLSTDVLDQVIELILLSGLDGVVACNTTVSREGLMTGKEKLDAIGEGGLSGAPLKE
                     KVLSVIRYIHEKTEGNLPIIGVGGIMTPQDAKDMLDAGASLVQVYTGFIYEGPCFARK
                     ILKYIS"
     gene            6401..7498
                     /locus_tag="PLM68_06220"
     CDS             6401..7498
                     /locus_tag="PLM68_06220"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT54990.1"
                     /translation="MIKKGLLILFTLTVMVVLLVSCNNKKDNAIRVIDELFTDMWPSG
                     EPGGVVLVLHKNKVVFTKGYGLATIDPETPATPSVNFCIASVSKQFAAVAILKLAQEG
                     KLSLDDPVSHYFPQFKAPFFSDIKLSQLLSHTSGIPDVRPRTDSLYVYHSTDVESYSY
                     LDTLSFLNFPPGSSYEYMNPTYQLLYTVIEKASGVPFEEYMRDEIFLPAGMSNTQYFE
                     SGRDIPSMAHGYRFNSEKGNWEEYDYGEASFFASKADGALYTSAEEFVLWEYALRNNT
                     ILGQEMTLEAHTSRIATDIPDTGYGYGWFISSINGAPKIFHTGDNGGFKIYAGRCPQS
                     ETLLLIFSTRDFEREETVEKVEGILVEAGWL"
BASE COUNT         2048 a         1694 c         1665 g         2158 t
ORIGIN      
        1 gtgcttttcc ttcccaacta cggctccaat gggatatccg tttgccatgg ccttgccaaa
       61 ggcggaaaga tcgggggtga caccgaacac tttctgggca cctcccagat catagcggaa
      121 accgctacgg atttcgtcaa aaacaaggac ggttccgtat ttgtcggcca gtgcccgtac
      181 accctccaga aaacccggtt ttggcatctg aaccttctcg gccaaaggat gccctaccgg
      241 tgtgatgatg atggcggctg tgtcgtctcc atgctgcttc atcagatcct ccagctcgtc
      301 cagctcgttg taatggaatt catagacatc ttcccatagt ttcctgggta tacctccctt
      361 gacttccaca caccagtcgt gccagccatg ataaccacag cggatcacct ttactctttc
      421 cgtgcttgcc cgtgcaaccc tgatggcaac cgatgttgcg tccgatccgg ttttcacaaa
      481 agatgccatc tcggccgaag gtatcagttc aatgagcttt tctgccaatt tgttttgtat
      541 aggttgtgtg agggaaaaac agaaaccttt gttctgtatt tgctcaataa ctgcattgtc
      601 tatctcttct tcacggtgtc ctataataat gggaccatag gcacaaagca tgtctatgta
      661 ttcgtttccg tcaacgtcgg taacccttcc tccttttgca tgatcgaaat atatgggata
      721 ttcaccaggc acgaagttat aaggacgacg gattccggct atagcaccgg gaatgatctt
      781 tagggctttc tgatacatat tatcggaaat ctccagcttc agtttttttg acatgatgat
      841 ctgatttata tgttatgatt atttacctac gatatagttc acaaatatgg attgtgtatg
      901 cacattttca ggcaggatat ctcctgtttt gacaatttcc ttggggtctg caattacaag
      961 atcggcagcc atggccatca ttacattgaa attccttgaa gtgcctctga aataaaggtt
     1021 tccctcttca tcggccatgc tggcgcctat gagggctatg tcggccttaa ggggtttttc
     1081 cagcaggtaa tctttcccat ccacgttgat tatgtctttt ccttctgcca ctatggtacc
     1141 aagtcctgta ggggtaagaa tccctcccag ccctgcacct gcgcagcgta tctgttcaat
     1201 aagcgttccc tgtggcacaa aaatgacctg cagttcaccg gaatggtatt gttccccggt
     1261 agcaggattt gttcctatat ggctggttat gacctttgat accctgtgtc gggctatcag
     1321 ttttcccgga cccttgtctg gaaatcctgt gtcgttggta atgatagtca ggtctttaac
     1381 gtctgtttcc aggagagcgt ccagtatgcg ctgaggactg cccaaaccaa gaaaaccgcc
     1441 aaccatgatg gtcatgccgg attttatgaa agatacagct tcctgcaagg aaatttgttt
     1501 gctcataaca gattgaaaaa ttaaaattga atcggcaaat ataacgataa attacgaatt
     1561 tcttgacaaa aatgtcttat ttttgtaagc aaaacctcca taatgataat aactgtcgag
     1621 aactttcttc tgataggatc cttattacta ttctttagtt tactggcggg aaaaaccggc
     1681 tacagattcg gggtccccac ccttttattg ttcctggtag tgggaatgat atttggaagt
     1741 gaaggcctgg gactccagtt ttccaatccg aaaactgcac agttcatagg cgttgtggca
     1801 ttgagtatca ttctgttttc gggaggtatg gataccaaat accaggagat caaacccata
     1861 gcaagacaag gaatcattct tgccactttt ggagtgttgc tcacagccat cctgaccagt
     1921 ttgttcattt acggacttac gttactcatc ccgggcttca acctgtcatt tccagtatgt
     1981 ctgcttctgg gaagcatcat gtcctctacc gattccgcct cggtatttgc catattgagg
     2041 tcaaaaggac tgaaactcaa acacaacctc aggcctcttc ttgaactgga aagcgggagc
     2101 aacgatccca tggcctatat gcttaccatc actatgatcc aggtgatcat gaccacggaa
     2161 ggtctcagga tctggcaagt tgtattgttg tttttctacc agatgatcac aggaggtctt
     2221 gcaggctacc tgctggggaa gctatgtgct tacctgatga gaaaattcaa catcggaaac
     2281 gactccctct accccattct aatactggcc ttctgtttct ttatctttgc ctttgcagac
     2341 ctgataaagg gaaacggctt ccttgccgtt tacataggag gacttgtgat tggcaattcg
     2401 cgtttcctgc agaagagaaa cgtcatgaac ttctttgacg gtctatcctg gctctctcag
     2461 atcatcatgt tcctggccct gggattgctg gtcaatcctt cagaactttg gcccatagca
     2521 ggtgtgggtt tgctaattgg tatcttcatg atcgtgctgg cccgccccat tgccgttttg
     2581 ttatcgcttt tcccgtttcg catgatgact accaactcaa ggatttttac aagctgggta
     2641 ggactcaggg gcgcagtgcc cataattttc gccacttatc cactggtaag cgggattgcc
     2701 gaggcaagca tccttttcaa catagtcttt ttcataacca tactttccct tctggtccag
     2761 ggtacttcca taccctggat tgctaaaaaa cttcatgtat ccatcccgca gaaaatcgcc
     2821 cgcagatccg agttcgaaat ggagttttcc gaggacataa aatctgccat gagcgaggtt
     2881 gtcgtcaccc aggaaagcct gcgtttcggg agcaggatga cggacattcc gctacccgac
     2941 aaaaccctgg ttgtaatggt taaaagaggt gattcttttt tcattcccag gggtggaaca
     3001 gagattcata tcggggataa actgctcatt atttcagatg atgagaaggc attaagggaa
     3061 acatacggaa aactaggtat ggattcagat atctgatata tataacaaat atgttatata
     3121 gtataacacg tttgttatat gtcgccttat ttatttgata ttctgtgttc tggattaaat
     3181 agcacactcg agtctccata actaagaaaa cggtagtttc cggccagtgc atggtcatag
     3241 atcaccctcc aatcctcccc tgtcagggct gccaccagca agagcaaagt agagttcggc
     3301 tggtggtaat ttgtgataag aatatcggtt gtcaggaacc tgtaggaagg agcaattatt
     3361 atctgcgttg atgcatccaa aatatctttt tgcaccctgt ccatataggc acgcaaatac
     3421 cccagtgatt ccctgtaagg catcccggag gaagttacat acggctccca ctgacccaca
     3481 aaagagggtg tcttgccccc tgctgcctgc agccccaggt aatagaggct ctccaggcaa
     3541 cggcatgaag tggttccaac agaaaccaca ggtcccctgt gcgcggtcaa ctgctcaagg
     3601 aactccctgg ttacactgaa gggttcacta tgcatgatgt gatccctgat gtaaggtgtt
     3661 tttaccggtc tgaaagtacc tgagcccaca tgtaaagtca attcagctat tcccatacct
     3721 cttttttgga ggtcggagag tactttctcc gagaaatgaa gtcctgcagt aggcgcagca
     3781 accgaacccc tcttccgtgc ataaatagtc tggtaacgct ccaggtccaa cgcctccgat
     3841 tctcttttca aatatggggg gaggggaacc cttcccgtag cctcaagcaa tgaagagaaa
     3901 ggcatgtttg cagtccagct gaaacggacc agatacctgt cggcttcctg accgataagg
     3961 cttgcagaca gtccgagtgc ggatagtttg ggatcgtttt gcggattata gagaggaagg
     4021 tttccggttt tccatttttt tgcattgcca acagcacaat accacgtgca ggaagtgtgc
     4081 tggctaaagg caaggatata ctccctggga gaggcgggct caaggcagaa tacttcaatg
     4141 acagctcctg tttctttccg gaaaaacaag cgcgcaggaa ctactttgga attattgaat
     4201 accaggaggc tttcttgtgg taaaagagaa ggaaggtcaa caaaaacatg gtcagaaacc
     4261 ctcccgtcac ggtagaggag taattttgaa tcatgacgaa ccggaagcgg atattttgca
     4321 atcttgttat ccggcaattc ataagaatag tcctttatgc gtacaccggg gataggaata
     4381 tggaatgttg tcataagtaa catcaaaggt atggtaaaat gagttgattt tatatctaaa
     4441 gttttacttt aacacatgtt ttacatttgt aatttactta tgtaatttcg tataatacaa
     4501 atgtctcttt tttgggtttt tgtttctctt ttttcctaag atacgaaaaa tacattggtt
     4561 tacatattta aacacttaca aatatacgta tttaatattt gataaccagt attttataag
     4621 ttatatctat atctttatgt gaacaacata tcgtgtcaac tgtgtttttt gacatgtttt
     4681 taattgaaat cagattaaat gattgtttat gtcactgttt accagttata tatattgatt
     4741 atataaagtt ctggtagatg atatgaccta tgactgctaa tctttataaa ttaattgatt
     4801 gttagtggtt tgaaatatgt ttatgaaaat aagtgtttac aaatgtattt tctataatag
     4861 ttacaattgt aaactatatt tgtattacat ttgtaaaaac taagtttaca attatgagaa
     4921 cgcgtttgca acaattttta acggcagaaa acatctctcc ttcgcgtttt gcagatcaga
     4981 tcgggatcca gcgttccggt ttgtcccaca tattggctgg aagaaataaa cccggatttg
     5041 actttattga gaaaatgctg cttacctacc cgtctctcaa cgctgaatgg ctaatcacag
     5101 gaaaaggaaa gatgtataga gaaaccaggg aaaaagaacc tgaactctta ccagaagaac
     5161 caccaattca ggaaacaaaa aacttatttg cagacactta caacatatta cctgaagcag
     5221 gacataagct gattaaagtg ttgttgttct atgatgacaa tacatttaca gagcataatc
     5281 ctgccgatgc atctctataa agattgcccg gtgcaattta tcctgtatat ttgtttcata
     5341 aactaaaaac aaatgtatcg tatcatcagg cccctattat tcttatttcc tcccgagact
     5401 gctcataatc tggcattcaa ggccttggca atagtaaaat acattcccct gggaaccagg
     5461 attctccggt cattgttctc gtataactcc ccatccctgc aaagacaggt aatgggaatt
     5521 gattttccga atccggtggg actggctgcc ggtctggata aaaaagcaga ggtatacaat
     5581 gaaatgggtg ctcttggttt tggttttgtc gaaattggct ctgtcacggt cgctccccag
     5641 gaaggaaatc ccaaacctag aagtttccgc ctggtaaaag acagggcttt aataaacagg
     5701 atgggaataa acaacccggg agtcaagcaa atagtgcgca atatccagca aaacaagccc
     5761 cgtgtcataa ttggcgggaa tattacaaaa aacacttcaa cccccaatga tcaggcatac
     5821 aaggaccttg aaaaagcctt tgcccagatg tacgactatg tggattattt tgtcgtcaat
     5881 gtaagttgtc ccaatgtcaa agacctgcgc agcctgcaga cggttgataa cctaacggga
     5941 ataaccaggc acctgacaga gatccgaagg tacagcgacg tataccgccc cattctgctt
     6001 aaggtgtccc cagacctgtc aaccgatgtg ctggaccagg taattgaatt gatcctgcta
     6061 tcgggactgg acggcgtggt ggcctgtaac acaacagttt ccagggaagg actcatgacc
     6121 ggaaaagaaa aactggacgc tatcggggaa ggaggcctga gcggggctcc actcaaggaa
     6181 aaagttctgt ctgtgatcag gtatattcat gaaaaaaccg aagggaacct gcccatcatc
     6241 ggggtgggag gaatcatgac tccacaggat gccaaagata tgctggatgc gggagcttcc
     6301 cttgtccagg tctatactgg tttcatatat gaaggcccct gttttgcgcg taaaatactg
     6361 aaatatatta gttagcagta attacttaaa ctcttgcttt atgataaaaa aagggctttt
     6421 aatactgttc acacttacgg taatggtagt gctgcttgtt tcgtgcaaca acaaaaagga
     6481 taacgctatc cgtgtaattg atgaattgtt cacagacatg tggccatccg gtgaaccggg
     6541 gggtgttgtg cttgttttgc ataagaacaa ggtggttttc accaaaggat atgggctggc
     6601 taccatcgat ccggaaacac ccgctacacc ttcagtcaat ttttgcatag catctgtatc
     6661 caagcaattt gcggctgttg ccatactgaa gctggcccag gaaggtaaat tatccctgga
     6721 tgatcctgtc tctcactatt ttcctcagtt caaggctccc tttttcagcg acataaaact
     6781 gtcacagctt ctttcccaca cttcagggat tccagatgtc agacctcgta ccgacagcct
     6841 ttacgtttac cattccacgg atgtagaatc atattcctac ctggataccc tgagtttttt
     6901 gaattttcct cccggatctt cctacgaata catgaatccc acgtatcagc tgttgtatac
     6961 ggtaattgaa aaagcctcgg gggttccctt cgaggaatac atgagggatg aaatattcct
     7021 gcctgcaggc atgtcaaaca cgcaatattt tgagtccggc agggatatcc cttcaatggc
     7081 ccacgggtat cgtttcaata gcgaaaaagg gaattgggaa gaatacgact acggcgaagc
     7141 aagttttttt gcatccaagg ctgacggggc tctttatacg tcggcagaag agtttgtcct
     7201 gtgggaatac gccctgagaa acaacacgat acttggacag gaaatgaccc ttgaagccca
     7261 cacttcccgc attgccaccg atatccctga taccggttac ggttacggct ggtttatttc
     7321 cagtataaac ggagcaccta agatcttcca taccggggac aacggaggtt ttaagatcta
     7381 tgccggcagg tgtccgcagt cagaaaccct gctgctgata ttctcgacca gggattttga
     7441 acgggaagaa acagtggaaa aagtggaagg tatcctggtt gaagcgggtt ggctataaaa
     7501 gccgcaggaa attgcccccc attatctttt ctatgtctgt aaaggaataa ccccttaaat
     7561 gcaat
//
LOCUS       DAOSOF010000096         7511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_9816_length_7511_cov_4.180258, whole genome shotgun sequence.
ACCESSION   DAOSOF010000096 DAOSOF010000000
VERSION     DAOSOF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9816_length_7511_cov_4.180258"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..435)
                     /locus_tag="PLM68_06225"
     CDS             complement(79..435)
                     /locus_tag="PLM68_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT54991.1"
                     /translation="MLKFILLSLAQTLLLASGQVCLKVSMECMDKFSFTWVYFRQLFT
                     NWYFALTGVFFIAATVLWMHILKHYPFSIAYPITSFAYVFGMIAALVIFREAIPPTRW
                     IGVALIVAGVFFLLKQ"
     gene            complement(429..1322)
                     /locus_tag="PLM68_06230"
     CDS             complement(429..1322)
                     /locus_tag="PLM68_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOT54992.1"
                     /translation="MGVYIQAAAQISVQNPLCDDWLDNPIQYQVPYQRSLEPDYKKFL
                     DPVASRRMGRILKRAIATSVTVAGSSGITNPDAIIVGTGLGCIETTEKFLLALVQEGE
                     EFLQPTNFINSTHNTIASHIAVHMKCNGYNSTYVHLGVSFESALLDAFMQFQLGRIHT
                     ALVGGHDEMIPNYFKILDKVGFWEGKMASECAVSFMLGDTPCRNPMARIREVVMLHNA
                     TAQREEEVINQVCERMETARNDWNTLPTVKPVFGESFTSQAFEMYAAAVKMQRGLIAD
                     NILLTNHYRGTETSLIFLSKC"
     gene            complement(1328..2524)
                     /locus_tag="PLM68_06235"
     CDS             complement(1328..2524)
                     /locus_tag="PLM68_06235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOT54993.1"
                     /translation="MKVYVTGFGIISALGLGAGSTLDALFNRQSGVGPLRWLETMHSH
                     LPCAEVPFSTQELRKMAGVAQDKVITRTALMGILALKEALEMALWNEHKPLNPVLISG
                     TTVGGMENTERFFLDFLSPDTSHDQYILAHDCGVCTQMIADHFGHFDMLSTLSTACSS
                     AANALVLAADMIRTGRTSMAVAGGSECLSKFHLNGFNTLKILDKDPCRPFDDTRAGLN
                     LGEGAAYLVLESEESAKARGVKPICLLSGYGNACDAYHQTASSPEGDGAVLSMKKALQ
                     EAGLSTQQIDYVNAHGTGTPNNDESEGKALMRVFGADTVPPVSSTKSFTGHTTSAAGS
                     VESVISILALLNNFLPVNLNYSNPMGMLSFRPVTDEKPSRPLSHVMTNSFGFGGNDTT
                     CIFSKI"
     gene            complement(2536..2793)
                     /locus_tag="PLM68_06240"
     CDS             complement(2536..2793)
                     /locus_tag="PLM68_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOT54994.1"
                     /translation="METLIFDLKKQIIEALNLEDVTPDDIDTNAPLFVEGLGLDSIDA
                     LELIVLMEKQYGIKLEDPGKSKEIFRSVATMADYISKNRQK"
     gene            complement(2795..3412)
                     /locus_tag="PLM68_06245"
     CDS             complement(2795..3412)
                     /locus_tag="PLM68_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT54995.1"
                     /translation="MKKITAYCRLTDDLVILNGKPCEISFPSEPGPGRLTDIYRHLEV
                     GYPKFFKMDNLSKAGFLASEMVLRSLQYDMETPDESTSIVFCNRSSSLDTDKRFQETI
                     SRDNYFPSPAVFVYTLPNIVTGEVAIRNKIMGETSFFILESFDAEKMIIFTDWAFSSA
                     GICRVLCGWTEYLDGHCNVLVMMVEKESDKGVDFNKQNINNIYRT"
     gene            complement(3409..4533)
                     /locus_tag="PLM68_06250"
     CDS             complement(3409..4533)
                     /locus_tag="PLM68_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT54996.1"
                     /translation="MIKPLAYNIVSPLGFTATDNYAAVRRGESGVVPCQDPVGIPIAV
                     SRLDRSKLLKAFEKKCKPSQKHTAPEQAMLLSASLAVQEAGIDASSARTGFVLSSTKG
                     NIELLGIPENDFGRERIHLWHNAALLSGFFNNPNIPLTVTNACISGAAAQIAAFNMLE
                     KGLYDHVVVTGVDMASDFIVAGFQSFKSLDPERCRPFDARRKGLNIGEAAATLVFGKA
                     DGKGISLIQGNVRNDANHISGPSRTGEGLYRALCHTLQDVPPRDIGFISAHGTATPFN
                     DQMEAIAFARAGLDHTPVFSLKAHFGHTMGAAGILESIISIMALEEGVVLPSMGYKEH
                     GVEGELNVSTVTAHTDKRFMVKTLSGFGGCNAALLYQKHS"
     gene            complement(4530..4958)
                     /locus_tag="PLM68_06255"
     CDS             complement(4530..4958)
                     /locus_tag="PLM68_06255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT54997.1"
                     /translation="MKTTHKLTESKQILIRFSEVDSMSIVWHGSYVLYFEDAREAFGR
                     KYGLTYQTYFDNGYFAPLVDMQFEFKNPLKYGDTARIEIEYIPVEAAKVIFRYNIYRE
                     KDNVLVATGSTTQVFLDRQYRLVLYSPDFYTEWKKKNNVL"
     gene            complement(4942..5388)
                     /locus_tag="PLM68_06260"
     CDS             complement(4942..5388)
                     /locus_tag="PLM68_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridylate synthase"
                     /protein_id="HOT54998.1"
                     /translation="MIQSANILELLPHRPPFVMVDRLTYCEGYHATTELLITVDNIFC
                     RNGFFSEAGLMENMAQTCAARMGFKSVAGNTRVKIGIIGAVKDFNVFFRPVPGDLLET
                     EIVIENEVFNAILLRAEIRCNGRTAATGHMKLFLTDIDSQPHEDNA"
     gene            complement(5385..6290)
                     /locus_tag="PLM68_06265"
     CDS             complement(5385..6290)
                     /locus_tag="PLM68_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HOT54999.1"
                     /translation="MISEKKPAWKGTTGGGNFGQRALLLLLRYVDIRAGYAILALVIP
                     FYMLFNRSGYRPIRDYFRRRGYSGNIIFHIYRNHYLFGQMMLDKFYLFARKKNPFTSR
                     VTDWDQFTSLLKSDGGFLIAGAHTGNFEICGYFLQQRLKKLNAVVFGGESAFLQAKRA
                     QALSGNMAELIPVMPDMSHLFVIKAALEKGEMVSMPCDRLFGSSKSFTFTFLGAPAHF
                     PMGAFLLAAQLEVPVCTLYVMREPARKYHIICKQLDVDRSQYKTSRDIARALCQVYVE
                     QLEDILKAYPHQWFNFYDFWNNTTL"
     gene            complement(6287..6535)
                     /locus_tag="PLM68_06270"
     CDS             complement(6287..6535)
                     /locus_tag="PLM68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOT55000.1"
                     /translation="MNRKEIIDIINKFLVEDIEVEPDALKEEAFLKEDLGIDSLDFVD
                     IVVVVEQNFGFKIKPEEMSKVKTLSQFYDYIERKLNAS"
     gene            complement(6554..>7511)
                     /locus_tag="PLM68_06275"
     CDS             complement(6554..>7511)
                     /locus_tag="PLM68_06275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOT55001.1"
                     /translation="CIDTDYLDRNEAGILFGNDSSSKAVIESHEICVAKKDTTLLGSG
                     AIFQSMNSTVTMNLSTIFRLRGINMTISAACASGSHSIGLGALLIRMGLQEMIVCGGA
                     QETNVLSMASFDGLNAFSTRTDQPQKASRPFDKGRDGLVPSGGAATVILEEYEHAVKR
                     GARIYAEVAGYGFSSNGMHISQPSEEGSAKAMALALADASLKPSDIDYINAHATSTVL
                     GDSYEALAIDRIFGKEKTPISSTKGMTGHECWMAGASEVVYTLLMMRDSFIAPNLNLE
                     EPDENSKKLNIIAKTLDRKLDICLSNSFGFGGTNSALILKKI"
BASE COUNT         1864 a         1837 c         1827 g         1983 t
ORIGIN      
        1 actggctgcg gtatgacacc gggcgacacg tagccggtaa aggtcagtag cagtgaaaag
       61 acgaggcgta tcatggcttt attgttttag caggaaaaac acacccgcca ctatcagggc
      121 aacgcctatc cagcgtgtag ggggtatggc ttccctgaat atgaccaatg cagcaatcat
      181 gccgaacaca tacgcgaaac tggtaatggg gtaggcaatt gaaaaagggt aatgtttcag
      241 aatatgcatc cacaggacgg tggcggctat aaagaacacc ccggtcaggg caaagtacca
      301 gttagtgaac agttgcctga aatacaccca ggtgaaggaa aacttatcca tacactccat
      361 ggatactttc aagcagactt gtccggatgc cagcaataag gtttgcgcaa gagataaaag
      421 tatgaatttc agcattttga taaaaatatc agggaggttt cagttcccct gtagtggttc
      481 gtcagcagaa tgttgtcagc aatcaacccg cgttgcatct ttaccgcggc ggcatacatt
      541 tcaaaagcct ggctggtaaa ggattcaccg aataccggtt tcacggtagg gagtgtgttc
      601 caatcgttcc ttgctgtttc catgcgctca caaacctggt ttatcacctc ttcttcacgt
      661 tgtgcagtag cattgtgtaa catgacgacc tccctgatgc gtgccatggg attcctgcag
      721 ggagtatcac ccagcatgaa gcttacggca cattcagaag ccattttgcc ttcccagaaa
      781 cctactttgt ccagaatttt gaaatagttg gggatcatct catcatgtcc ccctactaat
      841 gccgtatgaa tacgtcccag ctggaactgc atgaatgcat ccagcagcgc gctttcaaag
      901 gagacaccca gatgcacata agtgctgttg tagccgttgc atttcatatg aacagctata
      961 tgggacgcta tggtattgtg tgtggaattg atgaaattgg tcggttgcag gaattcctct
     1021 ccctcctgta ccagcgctag caggaatttt tctgttgttt caatgcatcc cagtcctgtt
     1081 cctacaatga tagcatccgg gtttgttatg cccgaagaac ccgccaccgt cacggaggtt
     1141 gcaatagccc ttttcaagat cctgcccatc cttcgcgaag caacaggatc caggaatttc
     1201 ttgtaatcgg gttccagcga cctttggtag ggaacctggt attgaatggg attgtccagc
     1261 cagtcgtcac aaaggggatt ctgtaccgaa atctgtgcag cagcctgtat atatacaccc
     1321 atagtggtca gattttagaa aaaatgcagg ttgtgtcatt gccgccgaac ccgaaggaat
     1381 ttgtcatcac gtgagacaag ggccgggaag gcttctcatc tgtgactggc ctgaaagaga
     1441 gcattcccat gggattgctg taattcaggt tgacaggcag aaagttgtta agcagggcaa
     1501 gtatggatat gaccgattca acgcttccgg cagcactggt cgtatgtccg gtaaaggatt
     1561 ttgtagagga gaccggtgga acagtatctg ctccgaatac acgcataagg gctttccctt
     1621 cactttcgtc gttgttgggg gtaccagtgc cgtgtgcatt tacgtaatct atctgttgcg
     1681 tggataatcc ggcttcctga agggcttttt tcatggaaag aaccgctccg tcaccttcgg
     1741 gggaagaagc agtttggtgg taagcatcac aggcattacc gtaaccagat aacaggcaaa
     1801 taggcttcac gccccgtgct tttgccgatt cttccgattc caggaccagg taagcagccc
     1861 cttcacccag gttgagccct gcccgcgtat cgtcaaaagg cctgcaggga tccttgtcca
     1921 gtatcttgag ggtattgaac ccgttcaggt gaaatttgct caaacattca ctccctcccg
     1981 cgacagccat ggaagtcctt ccggtgcgga tcatatcagc agcaagtacc agggcgttgg
     2041 ctgccgaaga gcaagctgta gaaagggtag agagcatgtc aaaatgtccg aaatggtctg
     2101 cgatcatctg ggtgcataca ccacagtcgt gggccaggat atactggtca tggctggtat
     2161 cgggggaaag aaagtccagg aaaaaacgct ctgtattctc cattcctcca acggtcgttc
     2221 ccgagatcag tacaggattc aaaggcttat gttcattcca gagggccatt tccagggctt
     2281 ccttcaaggc gaggatgccc atgagggccg ttctggtgat caccttatct tgagccacac
     2341 cggccatttt tcgcaattcc tgtgtgctga aaggtacctc ggcgcagggc aggtgggaat
     2401 gcatggtttc cagccagcgc aacggaccaa cgccgctctg ccggttgaac agtgcatcga
     2461 gtgtagaccc ggcacccaaa cccagtgcag aaataatccc gaatcctgta acgtatacct
     2521 tcattgccag ggtttttatt tttgacggtt tttactgatg tagtctgcca tggtggcaac
     2581 cgaacggaat atttccttgc tctttccggg atcttccagt ttgatgccgt attgtttttc
     2641 catcaacacg atcaactcca atgcatctat agagtccagg cccaggcctt caacaaaaag
     2701 gggagcattg gtgtcaatgt catccggggt aacgtcctcg agattcaaag cttcaatgat
     2761 ctgcttttta aggtcaaaaa tcaatgtttc cattttaggt tctgtaaata ttgttaatgt
     2821 tttgtttgtt aaaatccact cccttatcgc tttctttttc caccatcatt accagcacgt
     2881 tgcaatgccc gtccaggtat tcggtccagc cgcacaaaac acggcagata ccggcagaag
     2941 aaaaagccca atcggtaaat atgatcattt tttcggcatc aaatgattcg agtatgaaaa
     3001 aagatgtttc gcccatgatt ttgttccgga tggccacttc tcccgttacg atgttgggaa
     3061 gagtgtacac aaaaacagcc gggctcggaa agtaattatc ccggcttatg gtttcctgga
     3121 aacgcttatc ggtatccagc gaagaagaac ggttgcaaaa gacaatggaa gtgctttcat
     3181 ccggggtttc catatcgtac tgaagtgatc ggagcaccat ttcagaagcc aggaagcctg
     3241 ccttggagag gttgtccatc ttgaagaatt ttggataacc gacttccagg tgacggtaaa
     3301 tatctgtcag acggcccggg ccaggttctg agggaaacga tatctcgcaa ggttttccgt
     3361 tcagaattac caaatcatct gtcaagcggc aatatgctgt tatctttttc atgaatgttt
     3421 ctgataaagt aaggcggcat tgcatcctcc aaaacctgac aatgtcttca ccatgaatct
     3481 tttatccgta tgggccgtaa ccgtgctaac gttcagttct ccttcaaccc cgtgttcttt
     3541 atagcccatg gaaggcagga ccactccttc ttccagggcc attatggaaa tgatgctctc
     3601 cagtatccct gcggctccca tggtgtgacc gaaatgggcc ttgaggctga agacaggcgt
     3661 atgatcaaga cctgcccttg caaaggctat ggcttccatc tggtcattga agggagtggc
     3721 tgttccgtgg gcgctgatga atccgatatc ccggggaggc acatcctgca gggtgtggca
     3781 cagtgccctg tataatccct cgccggtgcg ggagggtcct gatatatgat tggcgtcgtt
     3841 gcggacattc ccctggatga gggatatgcc tttaccgtct gcttttccaa acacaagggt
     3901 agcggcagct tctcctatat tcagtccctt acgtcgtgca tcaaaggggc ggcaacgctc
     3961 cgggtccagt gatttaaagg actggaatcc agccacaata aagtcagaag ccatgtctac
     4021 ccccgttacc accacatgat cataaagacc tttttccagc atgttgaacg ccgcgatctg
     4081 tgcggcagct cccgatatgc aagcattggt gacggttaac ggaatgttgg gattattgaa
     4141 aaagccgctc agcagcgcgg cattatgcca caaatggatg cgttcccttc caaagtcgtt
     4201 ctccggaatg ccaagcagct caatattgcc ttttgtagag gaaagcacaa atcccgtgcg
     4261 tgcagaagac gcgtcaatac cggcttcttg cactgccaga gaggcagaaa gcaacatggc
     4321 ctgttcaggc gctgtatgtt tttgagacgg tttgcacttt ttttcaaatg ctttcaggag
     4381 ttttgacctg tcaaggcgtg atacggctat ggggatacct acagggtcct gacagggaac
     4441 gacaccgctt tcgcccctgc gcacggcagc gtagttgtct gttgcggtaa agccgagggg
     4501 agaaacgata ttgtatgcca ggggtttaat catagaacat tatttttctt tttccattcg
     4561 gtatagaaat cagggctata caacaccagc ctgtactggc ggtccagaaa gacctgggtc
     4621 gtggaacccg tggcaaccag cacattgtct ttttccctgt aaatattgta ccggaagatt
     4681 acctttgccg cttccaccgg tatgtactca atctcgatac gtgctgtgtc gccgtatttc
     4741 aggggattct tgaattcaaa ttgcatatcc accaggggag caaaatatcc attgtcaaag
     4801 tacgtctggt aggtcaatcc gtactttctt ccaaaagctt ccctggcatc ctcaaaatac
     4861 aggacatacg atccgtgcca gacgatgctc atggaatcca cttcgctgaa acggatgagg
     4921 atctgtttgc tctctgttag tttatgcgtt gtcttcatgg ggctgactgt caatatcggt
     4981 taaaaacaat ttcatatggc cagtggctgc cgtcctgccg ttgcaacgga tctccgccct
     5041 gagcaatatg gcattgaaga cttcattctc tatcacaatt tcagtttcca gaagatctcc
     5101 cgggacaggt ctgaaaaaaa cattgaaatc cttgacagcc cctatgatgc caatttttac
     5161 cctcgtgttg ccggccacac ttttgaaccc catgcgggca gcacaggtct gggccatgtt
     5221 ttccataagg ccagcttctg aaaagaagcc gttccggcag aaaatgttat caacggttat
     5281 gagcaactcg gtagtggcat gatacccttc acagtatgtg agccggtcca ccattacaaa
     5341 agggggacgg tgaggcagca attccagtat attggcagat tggatcatag ggtggtgttg
     5401 ttccagaaat cgtaaaaatt gaaccactgg tggggatatg ctttcagaat atcttccagc
     5461 tgttccacgt atacctggca taaggcgcgt gcaatatccc tggaagtctt gtattgtgaa
     5521 cggtctacgt ccagctgttt gcatattatg tgatatttcc tggccggttc acgcattaca
     5581 taaagcgtac acacgggtac ttccagctgt gctgccagca ggaatgcccc catgggaaaa
     5641 tgggcggggg caccgagaaa tgtaaaagta aacgatttgc tgctcccaaa aagcctgtca
     5701 cacggcatgc ttaccatttc acctttttcc agcgctgcct tgatgacaaa caagtgcgac
     5761 atatcgggca tcacagggat caactcggcc atgttccctg aaagggcctg ggctcttttt
     5821 gcttgtaaga aggccgattc gcctccaaaa accaccgcat tcagtttttt cagccgttgt
     5881 tgcaaaaaat acccgcagat ctcaaaattt cccgtatgtg ccccggcaat caaaaaaccg
     5941 ccatcgcttt tgagcagtga tgtaaactgg tcccaatccg tcaccctgga tgtgaaagga
     6001 ttctttttcc gggcaaagag atagaatttg tccagcatca tctggccaaa gaggtaatga
     6061 tttctgtata tgtgaaagat gatattccct gaataacccc tgcgtctgaa ataatcccta
     6121 atgggacggt aaccggaacg gttgaacagc atgtaaaagg gaatgacaag ggccagtatc
     6181 gcatatcctg ccctgatatc cacatagcgg agtagaagga gcaaggctct ttgtccaaaa
     6241 tttcccccgc ctgtggtccc cttccacgcc ggcttttttt ctgatatcaa cttgcgttaa
     6301 gttttcgttc aatatagtcg taaaattggg ataaagtctt cactttcgac atttcttccg
     6361 gttttatctt gaaaccgaaa ttctgttcta ccaccacgac gatatcaaca aaatccaggc
     6421 tgtcaattcc caggtcttcc ttgagaaatg cttcttcttt aagggcgtcc ggttccactt
     6481 cgatatcctc taccaggaat ttgttaataa tatcaatgat ttccttcctg ttcatgatct
     6541 tcttatgttc gtgctaaatt tttttcagta tcagtgcgct gttcgtgcct ccgaaaccaa
     6601 atgaattgga gaggcatata tccagtttcc tatctaaagt tttggcaatt atatttaatt
     6661 tttttgaatt ttcatcaggt tcctccaggt tcaggttggg tgcaataaag gaatcccgca
     6721 tcataagaag ggtatagacg acttcgctcg caccggccat ccagcactca tgacctgtca
     6781 ttcccttggt agaggagatg ggtgtttttt cttttccgaa tatcctgtca atggccaggg
     6841 cctcataact gtctcccaag accgtagatg tggcatgggc atttatataa tcaatatctg
     6901 agggtttcag ggaggcatcg gccagggcga gggccatggc ttttgccgag ccttcttcgc
     6961 tgggctgcga tatatgcatt ccgttggaag agaagccgta acccgccacc tcggcataaa
     7021 tgcgcgctcc gcgcttaacg gcatgttcat attcttccag gatgacggtg gcggcgcctc
     7081 cgctgggcac caggccgtca cggcccttgt cgaagggacg agatgctttt tgcggctggt
     7141 ctgtcctggt ggaaaaggcg ttcaatccgt caaagctggc catgctaagg acattggttt
     7201 cctgcgcccc tccgcatact atcatttcct gaagccccat gcggataagc agggcaccca
     7261 gcccgatgga gtgggaaccg ctggcacagg ctgcgctgat ggtcatgttg atgccccgaa
     7321 gcctgaaaat ggtggacagg ttcatagtca ccgtcgagtt catggactgg aaaatcgccc
     7381 ctgaacccag cagggtagta tctttcttgg caacgcatat ctcgtgggat tcaatgacag
     7441 ctttgctgga agaatcgttc ccgaacaaaa tgccggcttc gttcctgtcc aggtaatccg
     7501 tatcaataca c
//
LOCUS       DAOSOF010000097         7240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10335_length_7240_cov_5.234377, whole genome shotgun sequence.
ACCESSION   DAOSOF010000097 DAOSOF010000000
VERSION     DAOSOF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10335_length_7240_cov_5.234377"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="rpsC"
                     /locus_tag="PLM68_06280"
     CDS             <1..680
                     /gene="rpsC"
                     /locus_tag="PLM68_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT55002.1"
                     /translation="SRIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLKEELKKISN
                     KEVQINIFEVKRPELDATIVANNIARQIEGRVSYRRAIKMAMASTIRMGAEGIKVLIG
                     GRLGGAEMARREMFKEGRTPLHTFRADIDYALAEAHTKVGVLGIKVWICNGEVYGKRD
                     LSPNVGTAATAAGTGKMQGESRPGRGGRDRRGGDRREGRGGDRREGDRRDGDRRDGGR
                     RDRSPSR"
     gene            765..1901
                     /locus_tag="PLM68_06285"
     CDS             765..1901
                     /locus_tag="PLM68_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT55003.1"
                     /translation="MKIAYLSVFYPYRGGIAQFNANLFRELDKKHMVTAYNFTRQYPS
                     LLFPGKTQYVPPEDMAAMVPARAVLDSVNPFTYQRTARIIAEDRPDLLLMRYWMPFFA
                     PSLGWVARTLKNRGCTVVSIVDNLIPHERTFMDKPLSRWFLAQNHGCIAMSTSVREDI
                     LSLDPRIPCHLKNHPLYDHFGKKENPAEAKTKLGLDPDRKTLLFFGFIRDYKGLDLLL
                     EAFRDLGESYQLVIAGESYGSFDKYEEIIRSLPDTSVVKVFNRYISDAEVPSFFSAAD
                     VCVLPYRTATQSGISAMAYHFEVPVIVTPAGGLAEAVEGPGTGLVASGTDANSLNRAI
                     RKFYTMDRDVFVTNIRKEKNALTWERFAHDLSDFASRLKNPKTK"
     gene            1914..4019
                     /locus_tag="PLM68_06290"
     CDS             1914..4019
                     /locus_tag="PLM68_06290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOT55004.1"
                     /translation="MKKTLLLLLLMGLLTACTSDPMKNNPLLQEYETPFQTPPFDKIK
                     TEHYMPAMREAMAQHKAEIDAIVNNQEAPSFENTVAAYDRAGELLSRVNAAFYTKNSA
                     DTDEELQALAREITPEMTRHRNEIQLNPVLFEKIRTLYNQRAGLNLDAQQMRTLEKYY
                     DDFVRSGAALSEEDKEILKDINTQLSNLSLQFSQNLLAETNDFKMVVDNVEDLAGLPE
                     SNVNAAASLATSLGMEGKYVFTPNKPSWIPFLQYVHNRDLREKLYKGYYMRGDNNNQN
                     DNKEIVAQIVNLRVRRSKLLGFDTYADFVLVNNMAKTPDNVLSFLQRVWDPALKVAKE
                     ELAAMQKIADAEGAGFKLESWDWWYYAEKLRKEKYDLDEQQIKPYLKLENVRDGLFYV
                     ANKLYGLTFEKRTDIPVYNPSVETYEVKDRDSSHLAILYLDYPTRAGKNAGAWCSGLR
                     SYKKLPDGSEQFPIVTVTTNFPEPVGDQPVLLSWDEAETLFHEFGHALEGFFGRGDYD
                     RICGALPRDMVELPSQINEHWAIEPEVLQVYARHYQTGEPMPEDLIQKLVNSSTFNQG
                     FMTVEFIAAALLDMEWHAATEEQVYDVNDFEKNALKKYGLIDEIIPRYRSTYFSHIFS
                     GGYAVGYYVYLWAEILDADAFDAFKEAGDIFDQETAGKFRKHVLTEGGWGEPMDQYLL
                     FRGKQPTEIPLLRNRGLIK"
     gene            4025..5227
                     /locus_tag="PLM68_06295"
     CDS             4025..5227
                     /locus_tag="PLM68_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT55005.1"
                     /translation="MKTRFIILLLGVWAVSACTHTGADTAIPVTQPRSVGFDPERLAL
                     VDQVIWDAIESEDIPGAVLAVVRHDRIAYLKAYGNRQLIPDTLPMTVETVFDMASVSK
                     CVGTTLSIMQLAETGRLRLMDRVDQYLPGFQPWTDPETGNNVPITIEDLLTHTSGLPP
                     YGPTQELIERYGSPNPQGLMEYICQCPRDFRPKTGYQYSCLNFITLQNVLEKIIGETL
                     ALYAQEHVFDRLGLQHTTYMPQGETLALCAPTELQEDGLPLVGQVHDPLARLLNGGNS
                     GNAGVFSNAEDLAVIAVALLNGGEVNGRRILGPLTVKAMERVPEKVAVFGRTLGWGRE
                     GSWNGELFNPRTVYGHTGYTGTSMVIDPETDTAVILLSNRVHPYDDGSAVSMRTRVAN
                     IVAGAIVE"
     gene            5229..5921
                     /locus_tag="PLM68_06300"
     CDS             5229..5921
                     /locus_tag="PLM68_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55006.1"
                     /translation="MKVFSAFLVFLLGTFTAVRAQGVFLDIDDSRPYTELYLFPAYMT
                     GTIQFEDGTAYTGQLNILLASSTLCFLDEEGQPDLFTDKNQVSAILIGRNYFIPLLGH
                     YIQILDTDGNVMFGVEQYLELEAEKRYGAFGREEKSPNVTIQKRNLRSEGFNVETGGR
                     SLAVESTPYTVHQHAYLIRNKKAYALTRKSFMRFFPSKKALIEQYEKDNTVDYSNPDQ
                     ALMLFKFLTAGH"
     gene            complement(6203..>7240)
                     /locus_tag="PLM68_06305"
     CDS             complement(6203..>7240)
                     /locus_tag="PLM68_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOT55007.1"
                     /translation="AKVFPARMSNIKINDAGAIVDYEWARDEGVDVMYGTRRIYGALL
                     NATVATAPVAKSYTIANSNDKDAIMAFFDQEGFSYTVDGNVVKVTYPKGCMYINTNDY
                     NQIMDLKYVNLRALAVSQTGGRAENDTYFYPSSSSNAVAGTGSFWSRPDSDSDILTYY
                     EMCFIKAEVNMRMGNAAAAYTAYIEGINASFNRMQTKLQDWAASGTTNPDQHPMSQTE
                     INAYLASAAVAKNASQLTMADIMLQKTIAMGINMETWNDMRRFNYGAGNIGNFGQVYL
                     NYVRPAEFKATNKIPGTSPTQENYWFRRYSQCSHETNYNKDNTAASNPLALTFENGAI
                     WSDPVWWDKAE"
BASE COUNT         1873 a         1768 c         1899 g         1700 t
ORIGIN      
        1 tgtcccgcat agtgatagaa cggaccctta agctgattac tgttaccatc cacacagccc
       61 gtcccggaat catcatcggc aaaggcggac aggaagtgga caagctgaaa gaggaactaa
      121 agaaaatttc caataaagaa gtccagatca acatttttga agtgaagcgc ccggaactgg
      181 atgccaccat tgttgccaac aacattgcaa gacagataga aggccgcgtt tcctaccgcc
      241 gtgccataaa aatggcgatg gcttccacca tacggatggg ggctgaagga atcaaggtat
      301 tgataggtgg tcgtttgggt ggagccgaaa tggcccgccg tgaaatgttc aaagagggac
      361 gtaccccttt gcacacattc cgcgccgata tcgattacgc attggccgag gcccatacca
      421 aggtgggcgt tctgggcatc aaggtgtgga tatgcaacgg tgaagtatat ggcaaacgtg
      481 atctttcccc aaatgtggga accgcagcta cagctgcagg aaccggaaaa atgcagggtg
      541 aaagccgtcc gggaaggggt ggaagggatc gccgtggagg agatcgccgt gaaggaagag
      601 gcggagaccg cagggaagga gaccgtaggg acggagaccg cagggatggt ggaagaaggg
      661 atcgttcacc cagccgctaa acagccgaaa agacaaaatc aagccctccg ttcaaggagg
      721 gcttttttgt tgtttatact tgaaatttac tacttttgta atgtatgaaa atcgcttatt
      781 tatctgtttt ttacccttac cggggcggga tcgcgcaatt caatgccaac ctgttcaggg
      841 aactggataa gaaacacatg gtaacagcgt ataatttcac caggcaatat ccttccctgc
      901 tctttcccgg aaagacacag tatgttcctc cggaagacat ggcagcgatg gttcccgcca
      961 gggctgtcct ggattcagtg aatccattca cctatcaaag aacggcccgg atcatagcag
     1021 aggaccggcc ggacctgctg ctgatgcggt actggatgcc tttttttgcc ccttccctgg
     1081 gatgggtggc ccgtacgtta aaaaacaggg gctgcactgt cgtttccata gtagacaatc
     1141 tgataccgca tgaaagaacg ttcatggaca agcctctatc ccgctggttc cttgcacaga
     1201 accatggatg tatagccatg agcacttccg tgagggaaga tatactgtcc cttgatccgc
     1261 ggattccctg ccacctgaag aatcatccgt tgtacgacca tttcggtaaa aaggaaaatc
     1321 ctgcagaggc caaaacaaag ctgggacttg accctgatcg caagacactg ttattctttg
     1381 gattcatcag ggattacaaa gggctggacc tgttattgga ggccttcagg gatctgggag
     1441 aatcctacca gctggtgatt gccggtgaga gttacggaag ttttgacaag tatgaggaaa
     1501 taatccgttc ccttccggac acttctgtcg tgaaggtttt caaccggtat atatctgatg
     1561 ccgaggttcc ctctttcttt tccgctgcgg atgtctgcgt tttgccctac cggactgcca
     1621 cccaaagcgg catctcggcc atggcctatc attttgaggt tccggtgatt gtgacacctg
     1681 cgggtggact ggcagaagct gttgaaggcc cgggaaccgg actggtggcc tccggaacgg
     1741 atgccaattc actgaacagg gctatccgga aattctatac catggaccgg gatgtgtttg
     1801 tcaccaatat ccggaaagaa aaaaatgcac ttacctggga acggtttgct cacgacctga
     1861 gcgactttgc cagccgcctg aaaaatccaa aaaccaaata accaaaactt accatgaaaa
     1921 aaacactgtt gttattatta ttaatgggct tacttacagc ctgtacatct gaccccatga
     1981 aaaacaatcc tttattacag gagtacgaaa ctcctttcca gacccctccc tttgacaaga
     2041 tcaaaacgga gcattacatg cctgccatgc gtgaggcgat ggcccaacac aaggcagaaa
     2101 tcgatgccat cgtcaacaac caggaagcgc cctcctttga aaataccgtt gcggcctacg
     2161 acagggcagg agaacttctg agcagggtga atgctgcatt ttacacaaaa aacagtgccg
     2221 acaccgatga ggaactgcag gccctggccc gtgaaatcac cccggaaatg acccgccatc
     2281 gcaatgaaat acagttgaat cctgttctat ttgaaaagat caggaccctg tacaatcaac
     2341 gtgccggact gaacctggat gcgcagcaga tgcggacact ggaaaaatat tatgatgact
     2401 ttgtccggag tggagctgct ttatctgaag aggacaagga aatactcaag gatatcaaca
     2461 cgcagctgtc caacctgagc cttcagttca gccagaacct cctggcagag accaatgact
     2521 ttaaaatggt cgtggataac gtggaagacc ttgccggact gccggaaagc aatgtgaatg
     2581 ctgctgcaag cctggccaca tcgcttggaa tggaaggaaa atacgttttc acacccaaca
     2641 agccatcctg gatacccttc ctgcaatacg tccacaaccg tgacctgaga gaaaaactgt
     2701 acaaagggta ttacatgcgg ggcgacaaca acaatcagaa cgacaacaaa gaaattgtcg
     2761 cacagatcgt caacctgagg gtgcgccgct ccaaactcct gggattcgac acctatgccg
     2821 atttcgtcct ggtcaacaat atggccaaaa cacctgataa cgttctgtcc ttcctgcaaa
     2881 gggtatggga tcctgccctg aaagtggcta aggaggaact ggctgccatg cagaaaattg
     2941 ccgatgcaga aggtgccgga ttcaagctgg agagctggga ttggtggtat tatgcagaaa
     3001 aactgcgcaa ggaaaaatac gacctggacg aacagcagat caaaccctat ctgaagctgg
     3061 aaaacgtaag ggacgggctg ttttatgttg ccaacaagtt gtatggccta acctttgaaa
     3121 agcggaccga cattcctgtg tacaatcctt ctgtggaaac gtacgaggtg aaggacaggg
     3181 acagcagcca cctggccatc ctatacctgg attacccaac ccgtgcaggc aagaatgccg
     3241 gggcctggtg ttccggcctg cgcagctaca aaaaactgcc cgatggcagc gaacaattcc
     3301 cgatagttac ggtaacaacc aatttccccg agccggtggg tgatcagccg gtgttgctga
     3361 gctgggacga agccgagacc ttgttccacg agttcgggca tgccctggaa ggattctttg
     3421 gccgtggcga ttacgaccgg atatgcggag ccctgccgcg cgacatggtg gaactgcctt
     3481 cccagatcaa tgagcattgg gccatagagc ccgaagtgct ccaggtctat gcccgtcatt
     3541 accagacagg tgaacccatg ccggaagatc tgatacaaaa gctggtgaac agcagtactt
     3601 tcaaccaggg tttcatgacc gttgaattta tcgccgccgc gctgcttgac atggaatggc
     3661 atgctgcaac ggaagaacag gtatacgacg tgaatgattt cgagaagaat gcgctgaaga
     3721 aatacggact cattgatgaa atcatcccca ggtaccgctc tacctatttt tcgcacattt
     3781 tcagcggagg atatgctgtg gggtattatg tttatctgtg ggccgagata ctggatgccg
     3841 atgccttcga tgcctttaag gaggcagggg atatctttga ccaggaaacc gccggcaaat
     3901 tccgcaaaca cgtccttacc gaaggcggat ggggcgagcc catggatcag tacctgcttt
     3961 tcagggggaa acaaccaacc gagatcccgt tgctgcgcaa caggggttta ataaagtaac
     4021 accaatgaaa accaggttta taattttgct cctgggggtg tgggcagtta gtgcctgcac
     4081 ccacacggga gcggatacgg ccataccggt gacacaacct cgttctgtgg gatttgaccc
     4141 ggaacgtctt gctcttgtcg accaggtgat ttgggatgcc atagagtcgg aagacattcc
     4201 cggagctgtt cttgccgtgg taaggcatga cagaatcgct tatctgaaag cttatggtaa
     4261 caggcagctg atacccgata cacttcccat gaccgtggaa acggtgtttg atatggcgtc
     4321 tgtcagcaaa tgcgtgggta cgaccttaag catcatgcag ttggcggaaa ccggccggtt
     4381 gcgcctcatg gacagggtgg atcagtacct gcccggtttt cagccctgga cagatcctga
     4441 aacaggtaac aatgtcccta ttaccattga agacctgctt acccatacct cgggtcttcc
     4501 cccgtacggt cctacgcagg aattgattga gcgttacggt tctccaaatc cgcagggact
     4561 tatggaatac atctgtcaat gtccgaggga tttcaggcct aagaccgggt accagtacag
     4621 ttgcttgaat ttcataacat tgcaaaatgt gctggaaaag atcatcggag agacgctggc
     4681 cctgtatgca caggaacatg ttttcgaccg cctcggctta caacatacca cctacatgcc
     4741 ccagggagag acgctggctt tatgcgcccc cacggagttg caggaagacg ggttgcccct
     4801 ggtgggccag gttcatgatc cgctggcccg gttgctcaac ggggggaatt ccgggaatgc
     4861 aggggtattc tccaatgctg aggacctggc ggtgattgcg gtggccctgc tcaacggcgg
     4921 ggaagtgaac ggcagacgca tcctgggccc cctgacggtg aaagccatgg aacgtgttcc
     4981 tgaaaaggtg gctgttttcg ggcgtacact gggctgggga agggaaggct cctggaacgg
     5041 ggaactcttc aacccccgaa ctgtatatgg acacaccggg tacacgggga caagcatggt
     5101 gattgatccg gaaacggata cggctgtcat tttgctgtcc aaccgggttc atccctacga
     5161 tgacggttca gccgtaagca tgcgcacccg cgtggccaat attgtggccg gggcaatcgt
     5221 ggaatgagat gaaagtgttt tctgccttct tggtattcct gctggggacc tttactgctg
     5281 tccgggccca gggagttttc ctggacatag acgatagcag gccatatacc gaactttacc
     5341 tgtttccggc ctatatgacc gggaccatcc agtttgaaga cggtactgcc tatacaggac
     5401 agttgaacat actgctggct tcttccaccc tttgtttcct ggatgaggaa ggccagccag
     5461 acctgttcac agataaaaac caggtatcgg caatcctcat aggcaggaat tatttcattc
     5521 ctctgctggg ccattacata cagatacttg atacagacgg gaacgtgatg tttggggtgg
     5581 agcagtacct tgaactggaa gctgaaaagc gttacggggc attcggccgg gaagagaaat
     5641 ccccgaatgt gactattcag aaacggaacc tgaggagcga gggtttcaat gtggagacag
     5701 gaggaaggtc gctggctgtt gaatccaccc cctatacggt acatcagcat gcttacctga
     5761 tcaggaacaa gaaagcgtat gccttgaccc gtaagagttt catgcgtttc tttccttcaa
     5821 aaaaagcatt gattgaacaa tatgaaaaag acaacaccgt ggattattcc aatcctgacc
     5881 aggcactcat gttgttcaaa ttcctgacgg ccggacactg atactttgct gctatcgctt
     5941 actccacaag gaaacgggag cttttttgtt acgccgaggc tcccggttcc ttgtggtttc
     6001 agcgatagag acggatgacc agcacacttt atctttatgc cttttattca acgtgttatg
     6061 aaatgtacgc ctccttcatc tatgcattaa cggcaaagtc gtagtcacaa caaggctggg
     6121 aagcctcggc ataaaggaaa agcaaaaaaa gagaccggct aacaatccaa ccggtctctt
     6181 ttttagcagg gatacgctgc gtttattcag ctttgtccca ccatacggga tcgctccata
     6241 ttgccccgtt ttcaaaggtc agcgccaggg gattggatgc cgcggtattg tccttgttgt
     6301 aattggtttc gtgggagcat tggctgtaac ggcggaacca gtaattctcc tgtgtggggg
     6361 atgtcccggg gatcttattg gtggctttga attcagcagg tctgacgtaa ttcagataaa
     6421 cctgtccgaa attgccgatg ttgcctgcac cataattgaa ccgtcgcata tcattccatg
     6481 tttccatatt gatacccatg gctatggttt tttggagcat gatatcggcc atggtaagct
     6541 gggaagcatt ctttgcaaca gccgcactgg ctaaataagc attaatttcc gtctggctca
     6601 tggggtgctg gtccggattg gtggtgcctg aggcagccca gtcctggagt ttggtctgca
     6661 tacggttgaa actggcattt ataccttcaa tgtaagcagt ataggcagcg gccgcatttc
     6721 ccatgcgcat gtttacttct gccttaataa agcacatctc ataataggtc agaatgtccg
     6781 aatctgaatc gggacgactc cagaaagatc ctgtgcctgc aacagcatta gaggaagagc
     6841 tagggtagaa ataggtatca ttttctgcgc gtcctcctgt ctgtgataca gccagagccc
     6901 tcaaatttac gtatttaagg tccatgatct ggttgtagtc attggtgttg atgtacatac
     6961 aacctttagg ataagtgact ttgacaacat tcccgtctac cgtgtaagaa aatccttcct
     7021 ggtcaaaaaa cgccatgatg gcatctttgt cattggaatt ggctatggtg tagctttttg
     7081 ccacaggggc agtagccacg gttgcattca gcagtgctcc gtaaatcctt cgcgttccgt
     7141 acatgacatc aactccctca tcgcgggccc attcataatc gacaatggct cctgcgtcat
     7201 tgatttttat gttgctcata cgggcaggga acaccttggc
//
LOCUS       DAOSOF010000098         7125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10551_length_7125_cov_4.974823, whole genome shotgun sequence.
ACCESSION   DAOSOF010000098 DAOSOF010000000
VERSION     DAOSOF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10551_length_7125_cov_4.974823"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..697
                     /locus_tag="PLM68_06310"
     CDS             248..697
                     /locus_tag="PLM68_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55008.1"
                     /translation="MEYPRTIVAEGEDLLQYLPHRPPVLLVDTFFGRERYTSYTGYTP
                     HTGSLFCPGDYFLEEGLLEHAAQSIALAAGLERVQNQGQVEPGFVGSVNSFITYRKVP
                     SGSTIYTEIRELHLYPGFSVLQAEIFCKGKLVARGELKVVKITGPGT"
     gene            694..1242
                     /locus_tag="PLM68_06315"
     CDS             694..1242
                     /locus_tag="PLM68_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55009.1"
                     /translation="MKNLLIVCALILTACGAAGRIPWEEVRPLLSTEDSVQLYEFQML
                     HMNGTEEGLLLVSMNTVNRPRLVLTSFFGMSLFDLEGTAEGYQVHYVMDVLNRSRMLD
                     LLWDDFSLLFEPYSRKGFPPVRNDDGAIVMLMTGNGILHTVIRAGDHVNGYPSTILID
                     HPALRLSVRIKVLQDAEESLCH"
     gene            1217..1588
                     /locus_tag="PLM68_06320"
     CDS             1217..1588
                     /locus_tag="PLM68_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55010.1"
                     /translation="MLKNLFAIESVTAENTWAFLAKASVDPKHPVFQGHFPGVPVLPG
                     VCSLYLIRTCTELFLGHDLRYNTVDSCKFTAMIDPLRNEKITVQGDLKQAEGIIRIKA
                     LMLYGNRTVLKLQATMKEITL"
     gene            1585..2184
                     /locus_tag="PLM68_06325"
     CDS             1585..2184
                     /locus_tag="PLM68_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT55011.1"
                     /translation="MNIKKLILLATLAFPVTAWSQATEEQFYKELARISREIETLSGS
                     FTQTKEFRSLDVKVVSKGKFHYARNREIRFDYTVPYEVGLVVDDLTVKITSGDKTTTY
                     VLKDQKNAMAEMASIMNYCMKGLLYELKAGYDLAYAGDGPYHRVIVKNLKEDPANVFD
                     RIDLKFTRENYALEEMVISEKSGYITTYVFGNLAVNVAR"
     gene            2232..2639
                     /locus_tag="PLM68_06330"
     CDS             2232..2639
                     /locus_tag="PLM68_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55012.1"
                     /translation="MKVKDVPQEDPFVQPDRDVSLCGRLFAVDDDGKYQEVPSKGWSP
                     RNDAVRFAWNNWGEKAGKVREKVLAGKYSPLAFHMARVRMTSGMLAAYTGFSRRQINK
                     FCKPKNFASLDSGALSRLAEALYMSVEELISID"
     gene            2642..3652
                     /locus_tag="PLM68_06335"
     CDS             2642..3652
                     /locus_tag="PLM68_06335"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOT55013.1"
                     /translation="MEINFEHGHGAHCESAVVRNLLNFYGVRLSEPMVLGLGAGIFFC
                     HLPFFKVNGYAMTSFRYISGRIFSDVTKTLGIEYRKHSFKDPDEAMAKLDEVLLSGTP
                     VGLRVGVYNLTYFPKEYRVHFNGHNLCVIGKEGDDYIISDPIGQNKNKLSSADLKKVR
                     FAQGALPPKGSMYYITSMEHYKPLDTGMIEKSIIKTCYNMTHIPLPMFGAKGIAYLAR
                     RMRKWEKLFGKREAALNLAQIIRMLEEIGTGGAGFRFMYAAFLQEASVLLNRPALNQF
                     SMDLTAVGDLWRDFSYNAARFFKQREPEGLTYNDLAVKLEVISARELDIFKRLENSIK
                     RG"
     gene            3645..4403
                     /locus_tag="PLM68_06340"
     CDS             3645..4403
                     /locus_tag="PLM68_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55014.1"
                     /translation="MDNQFAIETRDLKKTYKGKPHPTLDGFSLRIPYGSLFGLLAPNG
                     AGKTTAINIICGLRHYDSGDIFIDGHDVSDGMQHVKPLLGLVPQDIALFPSLTAYENL
                     KIFGGLYGLGKKLLEERIDQLLELFDLSASRNSYINRFSGGMKRRVNVIAGMLHVPKI
                     LILDEPTVGVDAQSRRLILEKLTDINKAGTTILFISHYLEEAEQLCNRVAFMDNGAIV
                     CEGNPAELIAGKSECNCLESLYLLLTGKKMRDSQ"
     gene            4400..5671
                     /locus_tag="PLM68_06345"
     CDS             4400..5671
                     /locus_tag="PLM68_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55015.1"
                     /translation="MRKFRFLLYKDYLLLKRDVAGLLLMFLMPAILVVLMALLQDSSY
                     RSLTESSVPLLLVNRDQGELGEAIDKQIEASHIFQIDRKVNEAIPTLEELEQEVASGH
                     YTLGIYIPENTTESIRKNVARYVLAAFSGTMEKPSPDMVNFVIFIDPTTKVSFYTALM
                     STLKERTQKIEFEYVLNEISRQVNEFSPIPVSTEGFSTEQVHIDIRDARLEGRDLNPN
                     PVQHNVPSWTLFAIFFIVISLSGSIIRERQDGSFSRMMTMPCSYTEYLLSKAVVFTMV
                     SLIQFVLMLLIGVYLMPLFGMESLNLGTSPAALILLALSAAVAAIGYGIAIGNIANTI
                     HQSAIFGAISVVIMAAIGGAWIPTAVMTESLRNLSRISPIYWGLSGFMDVFMLNAGVM
                     AVLKECGVMLLFGLVCFAVAVTFNYRRRMDV"
     gene            complement(5643..5879)
                     /locus_tag="PLM68_06350"
     CDS             complement(5643..5879)
                     /locus_tag="PLM68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HOT55016.1"
                     /translation="MTQSLLWEYVWEGGLEQPSVQDLIITPLGGIILGELTHRAALAM
                     GKDGFNWYKIILTCILNPAFAINNGFRRPSYAGN"
     gene            complement(5896..6036)
                     /locus_tag="PLM68_06355"
     CDS             complement(5896..6036)
                     /locus_tag="PLM68_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HOT55017.1"
                     /translation="MKEQYRPTFTTFPVKDNDLFIVNYVGHPYQGSFYYNSARSQGAA
                     IW"
     gene            6214..>7125
                     /locus_tag="PLM68_06360"
     CDS             6214..>7125
                     /locus_tag="PLM68_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT55018.1"
                     /translation="MFDVSVQYIQSVHCLHPIGRLETTQSKHFEDTIKKCLERETFLV
                     INFSACPYLSSSGIRVLLNTSRELASAGGQLVLTGLSSEMLQVLQIAGLDSVFEICAD
                     DVQAVTCIEQCKRTEITLENIFPGKAPCETERVRDALDSCLYEWKHPQLAGFDTLYIA
                     AGEGALGETLQRKDAVTGFFVTLFHCTGFIPDDPAYAPDFRIVSDPAQGTLFVDHALS
                     TRGPYRVRVTVSDPEPVSIRHLLQYKGFSPPQAASSLCTMLCLDRNPQRPSLSVVFRQ
                     ENGTWEGGTFLLQKENVLQRGEYSEQTL"
BASE COUNT         1909 a         1575 c         1758 g         1883 t
ORIGIN      
        1 ttatatatat agtgtggatt ttaccctgta ttttgtggat caaacttggt tctgaacgtt
       61 ttggatctag ttgtctgtaa atatagagta aaaatgcgca ttttaaaaaa ataaaatatt
      121 tggaaagtat aaaataatta ttacaggcag ggtggattac atataacgta atgataataa
      181 gggaatagaa gaagcaaagt gtcaggaaaa ggaattattc atacattcgt gtactaaata
      241 cacgaacatg gagtatcccc gtacaatagt cgctgaagga gaagacctgt tgcaatacct
      301 tccccacagg ccgcccgtac tgctggtgga tacttttttc gggcgtgagc gctatacatc
      361 ctatacaggg tatactcccc atacaggatc tcttttttgt cccggcgatt attttcttga
      421 agaaggacta ttggaacatg cggcccaatc catagccctg gccgccggac tggaaagggt
      481 acaaaatcag ggacaggttg aacccggttt tgttggttct gtcaattctt tcatcacgta
      541 tagaaaggtt ccttccggaa gcaccatata tacggaaata cgcgaacttc atctgtatcc
      601 cggtttttct gtcctgcagg cagaaatatt ctgcaagggc aagcttgttg ccaggggtga
      661 gctgaaagtg gtaaaaatta caggacccgg tacatgaaaa acctgctgat cgtttgcgca
      721 ctcatcctta cagcctgcgg ggctgccggc aggattccat gggaagaagt caggcctctg
      781 ctcagcacag aggacagtgt gcagctgtat gaatttcaaa tgctccatat gaacgggaca
      841 gaggaaggct tgctcctggt aagcatgaac actgtcaaca ggccgcgact ggtactgacc
      901 tcattcttcg gcatgtcgct gtttgacctt gaaggtacgg ccgaaggcta tcaggtacat
      961 tatgtcatgg atgttttgaa cagaagcagg atgctggatc ttctatggga cgacttttcc
     1021 ctgctgtttg aaccttattc ccggaaaggt ttcccacctg tacgcaatga tgatggcgcc
     1081 atagtgatgc tcatgacagg aaatggtatc ctccatacgg tgatcagggc aggggatcat
     1141 gtcaatggtt acccgtcaac cattttgatc gatcaccctg ccttacgttt atctgttaga
     1201 ataaaagtgc tgcaagatgc tgaagaatct ctttgccatt gaatctgtaa cggcagaaaa
     1261 tacatgggcc ttcctggcca aagcatctgt tgaccccaaa catcctgtct tccagggtca
     1321 ttttccgggg gttccggtct tgcccggggt atgttcactg tacctgatcc gtacctgtac
     1381 cgaacttttt ctgggacacg atttacgcta caacacagtg gattcctgta aatttacggc
     1441 catgatcgat cctttgcgca atgaaaagat cacagtgcag ggagatctga aacaggccga
     1501 aggcatcatt cggataaagg cactgatgct gtacgggaac aggacggtat taaaattgca
     1561 agccactatg aaagaaatta cgttatgaat attaaaaaac ttatactgct tgccactctt
     1621 gcattccccg ttactgcctg gtcacaggca acggaagaac aattttataa agagctggca
     1681 cgcatttcac gggaaattga aaccctgagc gggtctttca cacagaccaa ggagttccgg
     1741 tcactggatg tgaaagtggt ttcaaaaggc aagttccatt atgccaggaa cagggaaatc
     1801 cgctttgatt atacggttcc ttacgaagtg ggcctggtgg tggatgacct taccgtgaag
     1861 ataacatccg gcgataagac aactacctat gtgctgaagg atcaaaagaa tgccatggcg
     1921 gaaatggcca gtatcatgaa ttactgcatg aaaggtcttt tgtacgagct taaggccggg
     1981 tacgatcttg cgtatgccgg ggatggtcct tatcacaggg ttattgtaaa aaacctgaag
     2041 gaagatcccg ccaatgtatt tgaccgcata gacctgaaat ttacccggga aaactacgcc
     2101 cttgaagaaa tggtcatcag cgaaaaatcg ggatatataa caacctatgt tttcggcaat
     2161 ctggccgtaa atgttgcgcg gtaagtgttc cgtactaaaa aatattatat ttgtgccaaa
     2221 ttacctgagt aatgaaagtc aaagatgttc cccaggaaga tccctttgtt caacctgacc
     2281 gggacgtaag cttatgtggt cgtctttttg cagtggatga cgatggaaaa taccaggagg
     2341 ttcccagcaa gggctggagt ccaagaaatg atgctgtcag gtttgcgtgg aacaactggg
     2401 gagaaaaagc aggaaaggtc cgggagaagg ttcttgccgg caaatatagc cctttggcct
     2461 ttcatatggc acgggttagg atgacatccg gaatgctggc cgcctatact ggtttttcac
     2521 gtagacaaat taataaattc tgtaaaccaa aaaactttgc ctcacttgat tccggagccc
     2581 tttcaaggtt ggcggaggcg ttgtacatga gtgtagaaga actcatctct atcgattgaa
     2641 tatggaaatc aattttgaac acggccacgg tgcacattgt gagagtgctg tagtaaggaa
     2701 tttgctgaat ttttacgggg tcaggcttag tgagcctatg gtactgggat tgggggcagg
     2761 gatctttttc tgtcatttgc ctttcttcaa ggtaaacgga tatgccatga cttcattcag
     2821 gtatatttcg ggaaggatat tctccgacgt caccaaaacg cttggcatag agtacaggaa
     2881 acactccttc aaagacccgg atgaggcaat ggcaaaactg gatgaggtgc ttttaagcgg
     2941 gaccccggtg ggattacgcg ttggagtgta taaccttact tattttccaa aagagtacag
     3001 ggtccatttt aatggccata atctatgtgt gataggcaag gaaggagatg attacataat
     3061 atcggatccc attgggcaga acaaaaataa actgtcttca gcggacctga agaaagtgcg
     3121 gtttgcccag ggggcgttac cacctaaagg aagcatgtat tacattacca gcatggaaca
     3181 ctacaaacca ctggataccg gcatgattga aaagtcaatt attaaaacat gctacaatat
     3241 gacacacatc ccgttgccta tgttcggagc caaaggcatc gcttatcttg cccggaggat
     3301 gcgtaagtgg gaaaaacttt ttggcaaacg ggaagcggca ctcaacctgg cacagatcat
     3361 ccgcatgctg gaggaaatcg ggacgggagg agcaggattt cgtttcatgt atgctgcatt
     3421 cctgcaggaa gcttcggtat tattgaacag gcccgcgctg aaccagttct ccatggatct
     3481 gacagcggtg ggtgacctgt ggagggactt ttcgtacaac gccgcacgat tcttcaaaca
     3541 acgcgaaccg gaaggtctta cctataatga tctggctgta aaactggaag tgatttcggc
     3601 gcgcgaactt gatattttca aacgactgga aaattcaatc aagcgtggat aatcagtttg
     3661 ccatagagac cagagacctt aagaagactt ataaaggcaa accgcatccc acacttgacg
     3721 gtttttccct gcgcataccc tatgggtcgc tttttggact gctggcccca aacggggcag
     3781 gcaaaaccac agctatcaat atcatatgcg gtttgcgaca ctatgacagc ggagatatat
     3841 ttattgacgg gcacgatgtg tcggacggca tgcagcatgt taaacctctt ctgggcctgg
     3901 ttccccagga tattgcactt ttcccctccc ttacagccta tgaaaacctg aaaattttcg
     3961 gaggcctgta cggattgggg aaaaaattac tggaagagcg aatagatcaa ttgctggaac
     4021 tttttgactt gtctgcaagc cgcaattctt acatcaaccg cttttccgga ggcatgaaac
     4081 gcagggtcaa tgtgatagca gggatgctgc atgtacctaa aattttgata cttgatgagc
     4141 ccacggttgg tgtagatgcc cagtcgcgca ggcttatcct tgaaaaattg accgatatca
     4201 acaaagccgg aaccaccata ctcttcatat cccattacct tgaagaagcg gaacaactct
     4261 gtaaccgggt ggctttcatg gataacggtg ctatcgtgtg cgagggaaac ccggcagaac
     4321 tgatagccgg caagagtgag tgcaactgcc tggaatcctt atacctgttg ttaacaggaa
     4381 aaaagatgag ggacagtcaa tgagaaaatt caggttttta ctctataaag attatctgtt
     4441 gctcaaaagg gatgtggccg ggttgctgct catgttcctg atgcccgcca ttcttgtcgt
     4501 actcatggcg ctgcttcagg acagctccta cagatcgtta acagagtctt ctgtcccgct
     4561 gctgctggtg aacagagatc agggggaatt gggagaagcc atagacaaac agatcgaggc
     4621 ctcgcacata ttccagattg acaggaaggt aaacgaggcc attcccaccc tggaagaact
     4681 tgaacaggaa gtggcctcgg gccattatac actgggtatt tatattcctg aaaatacaac
     4741 cgaaagcatt cgcaagaacg tggcaaggta tgtgctggca gcattcagcg gaaccatgga
     4801 aaagccctcc ccggacatgg tgaattttgt catttttatt gaccccacta caaaagtttc
     4861 cttttatacc gccctaatga gtacattgaa ggagcggaca caaaagattg aatttgaata
     4921 cgtcctgaac gaaatttccc ggcaggtaaa cgagttcagc cccattcccg tttccacgga
     4981 aggattctcc acagaacagg ttcatattga tatccgtgac gcacgcctgg agggaaggga
     5041 tctgaatccc aatcccgttc aacacaatgt gccttcctgg accttgtttg cgatcttttt
     5101 tattgtgata tcgctttcgg gaagcataat ccgggaacgg caggacggaa gtttctccag
     5161 gatgatgaca atgccctgtt cctatacgga gtatctgttg agcaaagccg tggtttttac
     5221 catggtttcg ttgatccagt ttgttctgat gctgcttatc ggggtatacc tgatgcctct
     5281 ttttggcatg gaatccctga acctgggcac ttcccccgcg gctttgatct tgctggccct
     5341 gtcggctgcg gtagcggcta tcggttatgg gatagccata ggaaacattg ccaataccat
     5401 ccaccagtct gccatttttg gtgcaatatc cgtagtgatc atggctgcca ttggaggcgc
     5461 ctggatcccg acggctgtca tgaccgaatc cctgcgtaac ttaagcagga tctctcccat
     5521 atactggggc ctttcaggtt ttatggatgt gtttatgctc aatgccggag taatggctgt
     5581 tcttaaggaa tgcggcgtga tgctcctttt tgggctggtt tgttttgcgg tggccgttac
     5641 attcaattac cggcgtagga tggacgtctg aatccattgt tgattgcaaa tgccgggttg
     5701 agaatgcacg tgagtatgat tttgtaccag ttgaatccgt cttttcccat ggccagggca
     5761 gctctgtgtg taagttcacc caatattatt ccccccaacg gagtgatgat cagatcctga
     5821 acacttggtt gttccaggcc accttcccag acatattccc acagaagcga ttgcgtcaag
     5881 gcaaaaaaag gatgactacc agatggcagc tccctgtgat cttgcagaat tgtaataaaa
     5941 gcttccctgg tagggatgtc caacgtaatt gacaataaac aggtcattgt cttttaccgg
     6001 aaatgtggtg aaagtcggcc tgtactgctc tttcattttg gtcggtttga acacttcttt
     6061 tctgtcccac tggcttatcg attccggata tactgcgctt gtattggcaa tacgaaaaac
     6121 agaatcacaa tgaacaaaaa aagtttatta aaaagtctca gcaaggcgga cggccttttt
     6181 caaagataat gagtttttta tgtaactttc gaaatgtttg atgtttctgt tcaatacatt
     6241 caatctgtac actgcctgca tcccatagga cgcctggaaa cgacacagag taaacacttt
     6301 gaagatacaa tcaaaaaatg ccttgaaagg gagacattcc ttgtgattaa tttctctgca
     6361 tgtccttatc tttcaagcag cgggataagg gtactgctga atacttcgcg ggagcttgca
     6421 tctgctggcg ggcagcttgt ccttaccggc ctttcctctg agatgttaca ggttttgcag
     6481 attgcagggc tggattccgt gtttgaaatt tgtgcagatg acgtccaggc tgttacctgc
     6541 atagagcaat gtaaaagaac tgagattacc ctggaaaaca tattccctgg taaggccccc
     6601 tgtgaaacag agagggtaag ggatgccctg gactcctgtc tgtatgaatg gaaacatcca
     6661 cagcttgccg gttttgatac cttgtacata gccgcgggag aaggggcctt gggagaaacg
     6721 ctccagagga aagatgcggt gacaggcttt tttgtcaccc tgtttcactg caccggtttc
     6781 atacccgatg acccggcata tgcccccgat tttcgtatag tcagtgaccc ggcacagggt
     6841 acgctctttg tggaccatgc cttgtccaca aggggccctt accgtgtacg tgtcacggtc
     6901 agcgatccgg aacctgtaag catcaggcac ctgcttcaat acaagggttt ttcaccgcca
     6961 caggcagcgt cttccctgtg taccatgctt tgtctggaca ggaatcctca aaggccttca
     7021 ctttctgttg ttttcaggca ggaaaacggg acatgggaag gaggaacctt tcttttacaa
     7081 aaggaaaacg tactgcagcg gggtgaatac tctgagcaaa ccctg
//
LOCUS       DAOSOF010000099         7120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10563_length_7120_cov_4.703751, whole genome shotgun sequence.
ACCESSION   DAOSOF010000099 DAOSOF010000000
VERSION     DAOSOF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10563_length_7120_cov_4.703751"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLM68_06365"
     CDS             complement(<1..805)
                     /locus_tag="PLM68_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOT55019.1"
                     /translation="MSKIDLSMYGISHVDEIVYNPSYETLFQAEMDPANEGFEQGVLT
                     KTGAVNVRTGIFTGRSPKDKYIVKDATTENTIWWDGVINKPISTDVWNSLKSLAKDQL
                     SGKKKLYVVDAFCGTNPDTRLKVRFIMEVAWQAHFVTNMFIRPSGYELEHFGEPDFVL
                     VNASKTTNPHWKEQGLHSDVFIVFNLTERMAVIGGSWYGGEMKKGIFSIMNYYLPLRG
                     IASMHCSANVGEEGDVAVFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHG"
     gene            909..2831
                     /locus_tag="PLM68_06370"
     CDS             909..2831
                     /locus_tag="PLM68_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOT55020.1"
                     /translation="MKQQEIFLQTLLRYWGYNSFRDTQEDIISSIYQGTDTLALMPTG
                     GGKSICFQVPALLMDGICIVVTPLIALMKDQVEKLKSLGIKALSVHSGMSSYEIDIAL
                     DNAVYGSYKFLYLSPERLGTELFRVRVHKMNPAMLVVDEAHCISQWGYDFRPSYLRIA
                     SFRELFPDVPFIALTATATPQVVEDIKEKLLFRKGSNVFQSSFERPNLAYVVRKTEDK
                     LGQLLRIAKGVEGTGLVYVRERKKAEEVAEFLIFNEIKADAYHAGMDARLRSSKQEAW
                     KKGDTRIMVATNAFGMGIDKPDVRFVCHFDPTDSPEAYFQEAGRAGRDGKKAYAVLLF
                     NPSDAKRMKQIFSVSFPDPDLIREAYQNLYSFLGLAYGEGAEQQFDFRLEDFAKRCKK
                     HPSTLWYALQCLEQEGYISLTEEVDNPSRIMFRVSRDELYRIQIANPRLDTFIKLLLR
                     TYTGPFNGFVSIDEQYLANVSRNAPAVITEYLMQLSRLSVISFIPKKKSPQVFLHSGR
                     LEPGNVYLNPATFLQRKDAYARRMEAMLQYAQSDAPCRSKQLLAYFGEGMKRDCDICD
                     VCLERQGKDNPNETFERIAGKITRTLRSGPVRIQQLDLLFSESAGKVVDVLRAMAENG
                     EVLIKDDTIQLNGNNT"
     gene            2859..4931
                     /gene="ligA"
                     /locus_tag="PLM68_06375"
     CDS             2859..4931
                     /gene="ligA"
                     /locus_tag="PLM68_06375"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT55021.1"
                     /translation="MNRSQAAARIRELKDAINEHNRKYYVLNTPDISDFEFDLLLQEL
                     TVLEKKYPDLAGDDSPTRHVGSDIAPENNAFLQFPHRYPMLSLSNTYNKEELLDFHNR
                     ISKVSDQPVEYVCELKLDGTAICLTYKDGRLFRALTRGDGTIGDDVTRNVLRIKSIPE
                     RLTPAGDFPYPPEFEIRGEIVMPFISFGELNSAKEKNGEPLFANPRNAAAGTLKLLSA
                     DEVERRGLDCYLYHLIADVPGIKTHWQALETASQWGFPVSQYRRLCRNVEEIMEFLEY
                     WDKARADLPIATDGVVVKVNSFSLQKTLGMTAKSPRWATAYKFKAEQAATRLLSVDFQ
                     VGRTGAITPVANLEPVLLSGTTVKRASLHNADQIALHDIRLGDMVYVEKGGEIIPKVI
                     GVELSSRPGNAQPFHYITHCPECGSLLERDPGEAKHFCPNRWECPPQIKGRIEHFMSR
                     KAMNILGGEALVEQLYDKQIVKDPADLYYLEPPMLEALENWGDKSTQNLLSSIEASKK
                     VPFHRFLFALGIPFVGETTAKYLASHFGSLQALKDAPIEDLTEAPEIGIKIASSIRDF
                     FENPVIGIMLDKLKDAGLPLRQDVTEKELMSESLQGLQFVISGTFSRSREDMKNLVQL
                     HGGKVLSGVSAHVDYLLSGDKTGPSKLQKASAMGIRIIGEKDFLALIGELPSEGTLFE
                     NENKTTSF"
     gene            4934..6148
                     /locus_tag="PLM68_06380"
     CDS             4934..6148
                     /locus_tag="PLM68_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT55022.1"
                     /translation="MTARILDTLHFIGTNDRNKPLFESNWPLPFGISYNSYLVTDKQT
                     ALLDTIEYGSDRSYFEDLELILNGRDLNYLVILHMEPDHAGMIGEVLQRYPLATIVTN
                     ARAIKLLDIYFPNIDKSRIREVKEGDTLELGHHHLQFVMTPMVHWPESMMAYCTTENI
                     LFSQDAFGTFGTLDGGIFDDEIDPEYYKDEMLRYYTNIIGRFSAQVEKTLQKAASLPI
                     RIICPVHGPVWRTNPRIVFDWYSQWAASKSQPGVVLVYATMYGHTQRTADYIARRLAE
                     NGVRNIRVFDVSKTHPSFILKEIWKYNGLVLGSCSYNTGMHPNMHHLCHEIEIMQPGR
                     KKAFLFGGYSWSGGGLKNLQLFVQKMTEQHKWELPVPGTELAGYPALNDLKNLDKDLA
                     TYAASLNNITTV"
     gene            6150..>7120
                     /locus_tag="PLM68_06385"
     CDS             6150..>7120
                     /locus_tag="PLM68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT55023.1"
                     /translation="MQNPDQIDLYFEPLRQSSLERCASEEEFKLVLKAYDFAKDAHKN
                     QKRISGDPIILHNIDVARIVVDEIGLGYKSIITALLHHILTDTEYTWEDISGMFGRKV
                     AELVVGLGKMERVFAAQDKTQEETFKQMLLTVNNDIRVLLIKLADRLHNLRNLQELPV
                     EKRARNLGESMYVFAPLAHQMGLYTIKSEIENIWMKHAMPSEYNEIEEKVKAEMGRQG
                     TEITQTYLGPIRDLLEHEGLEFTLSTRIKTPYSIWSKMKKRNVPFEEIYDLFALRIVF
                     VPMVTDIEQEHEMCYEIEQWLNQRWEPYTERRRDWLKTPKETGYEAL"
BASE COUNT         1969 a         1840 c         1666 g         1645 t
ORIGIN      
        1 cgccgtggtc gtcccagcca tgttcatcat ccccaatcag gtagcgttta ggatcggcag
       61 acaaagtggt tttgccggtt ccggaaaggc caaagaaaac agccacgtca ccctcttctc
      121 ccacgttggc agaacagtgc attgaggcaa tacccctcaa gggaaggtaa tagttcatga
      181 tggagaagat ccctttcttc atttcaccgc cgtaccagct gccgccaatc actgccatgc
      241 gttcggtcag gttgaataca atgaacacgt ccgaatgaag tccctgttct ttccaatggg
      301 gattggtggt tttggaagcg ttgaccagga caaagtcggg ttctccaaaa tgctccagtt
      361 cataaccgga aggacgtata aacatgtttg tcacaaagtg tgcctgccag gcgacttcca
      421 ttataaaacg cactttcagg cgggtatcgg ggttggtgcc gcaaaatgca tccaccacgt
      481 ataatttctt tttgccgcta agctgatcct tggcaagact cttgagggaa ttccatacat
      541 ccgtcgatat tggcttgttg ataacaccat cccaccagat agtgttttct gtcgttgcgt
      601 cttttacaat gtacttgtcc ttgggagagc gaccggtgaa gattcctgtc cttacattaa
      661 ctgcgcccgt ttttgtcagt acgccttgct cgaatccttc gtttgcgggg tccatctctg
      721 cctggaacaa agtttcatag gaagggttgt aaacaatttc atcaacatgg gaaatcccat
      781 acatggataa gtctatttta ctcattgtca attaattaat taaatgaaaa tctgaaagaa
      841 acgtcttatt tttctaactt gcgaagatac tcattttatt tatctttgca acagaaggca
      901 tgacgggcat gaagcaacaa gaaatattcc tgcagaccct gctccggtat tggggttaca
      961 attcgttccg cgatacccag gaagacatca tctcttccat ttaccaggga acggatacac
     1021 tcgctcttat gcccaccgga ggcggtaaat ccatttgctt ccaggtcccc gccctgctca
     1081 tggacggcat atgtatagtg gtcactccgt tgattgccct tatgaaggat caggttgaga
     1141 agctcaagtc actgggtatc aaggcgcttt ctgtccattc cggcatgagt tcctatgaaa
     1201 ttgacatagc actcgacaac gccgtttatg gctcgtacaa attcctgtac ctttcgcctg
     1261 aacggctggg tacagaatta ttcagggtgc gcgtgcataa aatgaatccc gccatgctgg
     1321 tggtagacga agcacactgc atctcccagt ggggatatga ctttcgaccc tcctacctgc
     1381 gcattgcctc attccgggaa ttatttcccg atgttccttt catagcactt accgccacgg
     1441 caacccccca ggtagtggaa gacatcaagg aaaagcttct ctttcgcaag ggaagcaatg
     1501 ttttccaaag ttctttcgag cgccccaacc tggcttatgt ggtcaggaaa accgaagaca
     1561 aactgggaca gctcctccgt atcgccaaag gggtggaagg aaccgggctg gtttatgtaa
     1621 gggagagaaa aaaagccgag gaggtggccg agttcctgat cttcaatgaa ataaaagccg
     1681 atgcctacca cgcagggatg gatgcccgcc tgagatcctc gaaacaggaa gcatggaaaa
     1741 agggtgatac acgcatcatg gtggccacga acgccttcgg catgggcatt gacaaacccg
     1801 acgtccgttt tgtctgccac ttcgacccta ccgattctcc cgaggcgtac tttcaggaag
     1861 ctggcagggc cggaagggat gggaaaaaag cctatgccgt gctgcttttc aatccatcag
     1921 atgccaaacg gatgaagcag atcttttctg tttcgtttcc tgaccccgac ctcatccggg
     1981 aagcctacca gaatctctat tcctttctcg gtctggccta tggagaagga gccgagcagc
     2041 aatttgattt taggctggaa gattttgcaa aacgatgcaa aaaacacccc tccaccctgt
     2101 ggtacgcact gcaatgcctg gaacaggaag gatacatatc tctgacagaa gaagtggaca
     2161 atccttcccg catcatgttc cgggtaagtc gcgatgaact gtaccggata cagatagcca
     2221 acccccggct ggacaccttc ataaaactgc ttttacgcac ctatacggga cctttcaatg
     2281 gttttgtatc aatagatgaa caataccttg ccaatgtcag ccgtaacgct ccggctgtga
     2341 ttacggaata ccttatgcaa ctttccaggc tgtcagtcat atccttcatc cccaaaaaga
     2401 aatctcccca ggtcttcctg cattccggca gactggaacc gggaaacgtt taccttaatc
     2461 ctgccacttt cctgcagcga aaggacgcct atgcaaggcg catggaagcg atgctccaat
     2521 atgcacaatc agatgctcca tgccgcagca aacaactcct tgcctatttc ggggaaggaa
     2581 tgaaacgaga ttgtgatatc tgtgacgttt gcctggaacg ccagggaaaa gacaatccga
     2641 acgaaacatt tgaacgtatt gccggcaaga tcacccgtac actacgttcg ggtccggtcc
     2701 gtatccagca gctggacctg ctcttttcag agtctgccgg taaagtggtt gatgtgttgc
     2761 gtgccatggc cgaaaacgga gaagtactca tcaaggacga caccatacaa ctgaatggca
     2821 acaacacata atatcaaaca acttcgtatc tttaccgtat gaatcgcagt caggcagccg
     2881 ccaggatcag ggagttgaaa gacgccatca atgagcataa cagaaagtac tacgtactga
     2941 acacacctga tatatcagat tttgaatttg atttactgct gcaggaacta accgttctgg
     3001 aaaaaaagta tcccgacctt gccggtgacg actcccccac ccgtcacgtt ggcagcgaca
     3061 ttgccccgga aaacaatgca ttcctgcagt ttccacacag atatccaatg ctgtcactaa
     3121 gcaataccta caacaaggag gaattgctcg acttccacaa ccgcatatca aaagtgtcgg
     3181 atcaaccggt ggaatatgtc tgcgaactta aattggacgg cacagccatc tgcctaacct
     3241 acaaggacgg acgcctcttc cgggcactca cacgcggcga cgggaccata ggagatgatg
     3301 tgacccgaaa cgtactccgc atcaaatcca tacctgaaag attgacacca gccggcgact
     3361 ttccctatcc ccccgaattc gaaatacggg gtgaaatagt catgccattc atcagtttcg
     3421 gcgaattgaa ctcggcaaaa gaaaaaaacg gtgaaccact ttttgcaaat cctcgcaatg
     3481 ccgcggcagg cacactgaaa ctgctttcgg ctgatgaagt ggaacgacgg ggtctggatt
     3541 gctacctgta ccatctgatt gcagatgttc cgggtatcaa aacccattgg caggctttgg
     3601 aaacggcttc ccaatgggga ttcccggtat cacagtaccg caggctctgc cgcaatgtgg
     3661 aagagatcat ggaatttctg gaatactggg acaaagcccg cgcagacctt cccattgcca
     3721 ccgatggtgt agtggtcaag gtcaacagtt tttcattgca gaaaacactg ggaatgacgg
     3781 ccaaaagtcc acgctgggcc accgcctata aattcaaggc ggaacaggcc gccacacgcc
     3841 ttttgtcggt cgatttccag gtgggaagaa caggagccat cacaccagta gccaacctgg
     3901 aaccggtcct gctttcagga acaaccgtta aacgggcctc tttacacaac gcagaccaga
     3961 tagcccttca tgatatccgt ctgggagata tggtttatgt ggaaaaagga ggcgagatca
     4021 tacctaaggt cataggagtg gagctttctt ccagacctgg aaatgcccag cctttccatt
     4081 acatcacgca ttgccctgaa tgcgggtctc tcctggaaag agatccggga gaagccaaac
     4141 atttctgtcc caaccgatgg gagtgcccgc cacagataaa aggcaggata gaacatttca
     4201 tgagccgcaa agccatgaac atcctgggtg gagaagccct ggttgaacaa ctctatgaca
     4261 agcagattgt aaaggacccg gcagacttgt actaccttga accccctatg ctggaggcac
     4321 tggaaaactg gggggataaa tccacccaaa acctgctcag cagcattgaa gcttctaaaa
     4381 aagtaccctt tcaccgcttt ctttttgccc tgggaatacc ctttgttgga gaaaccacgg
     4441 ccaaatacct ggcttcccac ttcggctcac tccaggccct gaaggatgca cccattgaag
     4501 atcttaccga agcacccgaa attggcataa aaatagcctc ttccatccgg gactttttcg
     4561 agaatcccgt gattggcatc atgctggata aactcaaaga tgcaggcctg ccgttaaggc
     4621 aggatgtgac agaaaaagaa cttatgtcag aatcactgca agggctgcag tttgtcatat
     4681 caggtacctt ttcccgttca cgcgaagaca tgaagaatct tgtccaattg catggaggca
     4741 aggttctttc gggcgtttcg gcccatgtgg attacctgct ctcgggagat aagacgggac
     4801 cttcaaaact acagaaagcc tctgccatgg gaataagaat aatcggtgaa aaggattttc
     4861 ttgcccttat tggcgaattg ccttctgaag gcacactttt tgaaaatgaa aataaaacca
     4921 catcgtttta atcatgaccg ctagaatcct cgacaccctg catttcatag gtacaaacga
     4981 caggaataaa ccgttgtttg aaagcaactg gcccctgccc ttcggtattt cttacaactc
     5041 ctaccttgtc acagacaaac aaacggcttt gctcgatacc atcgaatatg gaagcgaccg
     5101 ctcctacttt gaggatcttg agcttatcct taacggacgc gacctgaatt acctggtcat
     5161 cctgcatatg gaaccggacc atgccgggat gattggagaa gtgctgcagc gctatccctt
     5221 agcgacaatt gtcaccaatg cccgtgctat caagttactg gatatatact ttcccaacat
     5281 tgacaaaagc aggatcaggg aggttaaaga aggtgacacc ctggagctgg gacatcatca
     5341 cctgcagttt gtaatgactc ccatggttca ctggcccgaa tccatgatgg cttattgcac
     5401 aacagagaac attctttttt cacaggatgc cttcggaaca ttcggaaccc tggacggagg
     5461 catatttgac gacgagattg acccggaata ctacaaggat gagatgctca ggtactatac
     5521 caacatcata ggacgctttt cagcccaggt ggaaaaaacc cttcaaaagg ccgcttccct
     5581 gcccataagg atcatctgcc cggttcacgg tcctgtctgg cgtaccaatc cccgtattgt
     5641 gttcgactgg tacagccagt gggctgccag taaaagccag ccgggagttg tcctggtcta
     5701 cgccaccatg tacggccata cgcagaggac agcagattac atagcccgca ggctggctga
     5761 aaacggcgtc cgcaacatcc gcgtatttga tgtatccaaa acccaccctt ccttcatact
     5821 caaagaaata tggaaataca acggccttgt tctcgggtcc tgttcctata atacgggcat
     5881 gcaccccaat atgcaccacc tgtgtcacga aattgagatc atgcagcctg gaaggaaaaa
     5941 agcattcctt tttggagggt actcctggag cggagggggc ctgaaaaacc ttcagctgtt
     6001 tgttcaaaag atgacagaac aacacaaatg ggagctcccg gttcccggaa cggaacttgc
     6061 cggctatccg gcactgaatg acttgaaaaa cctggacaag gaccttgcca cctatgcagc
     6121 atccctgaat aacataacaa ccgtataatt tgcaaaaccc tgaccagata gacctgtact
     6181 ttgaaccgct caggcaatca agcctcgaac gttgcgccag cgaagaagaa ttcaaactgg
     6241 tactcaaggc ttacgacttt gccaaagatg cgcacaagaa ccagaagcgc atctcgggtg
     6301 atcccataat cctccacaac attgacgttg ccaggattgt ggtggatgag ataggtctgg
     6361 gttacaaatc catcatcacg gccctcctgc accacatact gaccgatacg gaatacactt
     6421 gggaagacat atccggcatg tttggcagga aagtggcaga actggtggta ggtttgggaa
     6481 aaatggaacg cgtctttgcc gcacaggaca agacccagga agaaaccttc aaacagatgc
     6541 tgcttacggt gaataacgat attcgtgtcc tgctcatcaa actcgccgac aggctccaca
     6601 acctgagaaa cctgcaggaa ttgcctgttg aaaaaagagc ccgcaacctg ggagagtcca
     6661 tgtatgtttt cgctccgctg gcacaccaga tggggcttta tactatcaaa tccgagattg
     6721 aaaacatatg gatgaaacac gccatgccct cggaatacaa cgagatcgag gaaaaggtaa
     6781 aagccgagat ggggaggcag ggaacagaga tcacccaaac ctacctggga cccatccggg
     6841 accttctgga acatgaaggc ctggaattca ctctttccac ccgcatcaag acaccatatt
     6901 ccatctggtc caagatgaaa aaacggaacg ttccttttga ggaaatctat gacctctttg
     6961 ccctgcggat cgtttttgta cccatggtga ccgacattga acaggaacac gaaatgtgtt
     7021 atgaaatcga gcaatggctc aaccagcgct gggaacctta cacggaaaga agaagggact
     7081 ggcttaaaac acccaaggaa acaggatatg aggcccttca
//
LOCUS       DAOSOF010000100         7037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10740_length_7037_cov_4.336293, whole genome shotgun sequence.
ACCESSION   DAOSOF010000100 DAOSOF010000000
VERSION     DAOSOF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_7037_cov_4.336293"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="PLM68_06390"
     CDS             <1..1567
                     /locus_tag="PLM68_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT55024.1"
                     /translation="VGGSQPVNGTNGAPAELSERTLREVFFPPFQACVDAGVETFMMA
                     HNEIEGIPCHTNPWLMQDVIRKEMGFDGFIVSDWMDMEHVYDLHRTAVSVEDAYAQSV
                     LAGMDMHMHGPGFVPAMLKLVEDGVVPVSRIDQACRRILELKFRLGLFENPFFDTTAV
                     QQTLRQEEHVQTSLEAARKSIVLLENNGILPLADGGKYKNVFVTGPNADSQGVMGDWT
                     FPQPDENVATFLEGLRMVAPKTKFTFLDQGESIRYMDPEKVRHAAEMARRADLSIVFV
                     GEYVNRKDWGNKTSGEDVERGNLNLPGLQEELIRNVLSSGKPCIVVLNNGHPLAVEWI
                     AQQADALVEAWEPGMKGGIALAEILYGLVNPTGKLPVTFPRSAGNILSVYNHMPSHYF
                     HPIVMEDPMPLYEFGYGLSYTTYRYDNLTPGTFLDGTLEVKVDVTNTGSVAGEEIVLL
                     YVRDDYASMTRPVKELKAFTRVALEPGETKTVSLKVGQEQLKFWTRDRGWITESGTFT
                     VMAGNLFAQVTLS"
     gene            complement(1554..3137)
                     /locus_tag="PLM68_06395"
     CDS             complement(1554..3137)
                     /locus_tag="PLM68_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT55025.1"
                     /translation="MKKDHGKRIILLMAVSLLFRSIVAALTELGNDEVYYWTYALFPD
                     WSHFDHPPMVGWIIQLFTCNLKWDSSFALRLPSLVLGTFNTWLIYRLGYLVKGPKTGW
                     YAALLYTGSLYAAIMAGTFILPDTPLGTFYLAALYCFFKAFGLGQNGDQKRNSGFFLL
                     AGVFTGLAMLSKYTGIFLWAGAILYLLRYRGKFFRESRLWAGLGISLLLFMPVLLWNL
                     SQDMSSLAFHSARLSISGEGFKPFYLLREISGSILYNNPVNVLLVIWSVFYYLRLKRL
                     KTTSSLPVRPEIFQMIIFQSVPLIAVFLFFSLFRETLPHWSAPGYYPLMLLAASVADH
                     KDLRWPAGLSPAILAIVVLLSVFQIKTGFIPIREMVPPREITPQDPYKELGRYDFTLD
                     MYGWTQLGDHFAQVKEKQEALFLESGGRRGMPPGSAILATRWFPGAHLDYYVARPAGT
                     VVKTTGPLEKTHKYEQITRKRGGIAPGEQLYYIESSRFPENMTGTVPLDTFFVQRSGK
                     PVIRYVVHQITSMADFRTE"
     gene            3199..4053
                     /gene="rfbD"
                     /locus_tag="PLM68_06400"
     CDS             3199..4053
                     /gene="rfbD"
                     /locus_tag="PLM68_06400"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT55026.1"
                     /translation="MKVVLTGANGGVGRIIQQLAPGQSRMELAAYSRQELNILDKQAL
                     TDCLSSLKPEYLINTAAFTHVDRAEKEPEAAFLLNAGCLEGMAEACRKTGTILIHLST
                     DYVFDGTKRTPYTEDDIPAPVNTYGASKRAGELIVLSYSRGIVIRTSWVYSRYSHNFL
                     AAIPLLLRTRSEPLQVDTLQVNSPTYAPDLVNALFTLAREGISHGLFHFTNTGGGCTR
                     YDFACHLREMINRKDPGAALAQVLPMHAEVQGGAPRPVYSVMSPAKIAALTGITIPHW
                     KEGIENII"
     gene            4061..5785
                     /locus_tag="PLM68_06405"
     CDS             4061..5785
                     /locus_tag="PLM68_06405"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT55027.1"
                     /translation="METSIMEKAREWLDPLYDEQTRKEVSRLMEEDPVLLEDSFYRNL
                     DFGTGGLRGIMGVGTNRMNKYTVGMVTQGLANYIKKTYPEPHKAAISYDCRNQSREFA
                     EITARVLAANDFTVYLFDALRPTPELSFAVRKLECHMGVMITASHNPKEYNGYKVYGN
                     DGAQVVPPDDELIIEQVRKVTSPAMVSFTGEGGKIISLGEAMDNAYLDSILSLVNLSP
                     DTINKYTNMKMVYTPLHGAGVRIVPMALEKMGFTHVIRVPEQEVPDGNFPTVVSPNPE
                     EPEALKMAINKAMETGAQLVMATDPDTDRIGIAVRNQGGDMELLNGNQTASLLTYYLL
                     EKWKETNRLKGKEYIVKTIVTSDLLQVIADHYGVSCYNVLTGFKYIAEIIRKNEGEKI
                     FICGGEESYGFNVGEFVRDKDAVVTCVLVAEVACWAAARGKTLHDLLKEMYVRFGFFK
                     ERMVSRTLKGKDGMEQIKAIMKSFRENPPEQLLGSPVVLMHDYKKSETVDLVSDLRYQ
                     ITLPKSDVIQFICADNTVVTVRPSGTEPKIKYYYGVQTQLVSAAAFRETEVLLDKKLT
                     DLHACFQN"
     gene            complement(5776..6309)
                     /locus_tag="PLM68_06410"
     CDS             complement(5776..6309)
                     /locus_tag="PLM68_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOT55028.1"
                     /translation="MNHKASLLFTWILIMATGCTLQEPVVSRKNVLKEIDIERITGTW
                     HEVAQWGRPERSQIAAVTLDVSMEDNGIIRLNIKKNKYTPYGPLTEIKGRAKWKADNP
                     GIFKSTLFVNLSRKCYILYLDPDYTASLFCNNSATKLWIFSKDKYLTENQWAEILQRM
                     ESFGLDPAKLVWHHNQF"
     gene            complement(6315..>7037)
                     /locus_tag="PLM68_06415"
     CDS             complement(6315..>7037)
                     /locus_tag="PLM68_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55029.1"
                     /translation="EGSLDLAIDHMEINLVKNQVIPLSNVHHIRFTRISGKYISILFN
                     SNFYCIFKSDSEVSCNGFLFAGYANPLPMQLTGSQIHALGSIVSDIRLEYKEDDSLKE
                     EMLRLHLKRFIITYTRIARKKYAIEPAHESALYLFRQFIILVDSNFKEKKKVGDYAQM
                     LNRSPKTIANLFSLYKLPSPLQIIQERIETEAKRLLLYSNMNAKEIAHILGFKEISSF
                     SRFFKRVSGKTITEFRNQYKER"
BASE COUNT         1886 a         1620 c         1818 g         1713 t
ORIGIN      
        1 tgttggtgga tcgcaaccgg tcaacggcac caacggtgca cccgcagaac tatccgaaag
       61 aacattgcgg gaagtcttct ttcccccgtt ccaggcctgt gttgacgccg gggtagagac
      121 tttcatgatg gcccacaacg aaatcgaagg aatcccttgc cataccaacc cctggctcat
      181 gcaggacgtc atccggaagg aaatgggttt tgacggtttc attgtcagtg actggatgga
      241 tatggaacat gtttatgacc tccaccggac agccgtatcg gttgaggatg cctatgccca
      301 atcggtgttg gcaggcatgg acatgcatat gcacgggccc ggatttgtgc cggccatgct
      361 taaactggtg gaagacggtg tggttcctgt gtcaaggata gaccaggcct gtcgcaggat
      421 cctggagttg aaattccgcc tcggcctgtt tgaaaatcct tttttcgata ccaccgcggt
      481 tcaacaaacg ctccggcagg aggaacatgt gcagacgtct ttggaggctg cccgcaaaag
      541 catagtattg ctggaaaaca acggaatcct gccacttgcc gatggcggaa agtataagaa
      601 tgtatttgtt accggaccca atgccgattc acagggcgtc atgggagact ggacgttccc
      661 gcaacccgac gaaaatgtgg cgactttcct ggagggactc cggatggttg ctcccaaaac
      721 gaaattcacg ttccttgacc agggcgagtc catccgttat atggatcccg aaaaagtgcg
      781 tcatgcagca gaaatggccc gcagggcaga tctgagcatt gtctttgtgg gcgagtacgt
      841 gaaccgcaag gactggggca acaagacttc gggggaagat gtggaacggg gcaacctgaa
      901 cctccccgga ttgcaggagg aattgatacg caatgtgctg agcagcggga aaccgtgtat
      961 cgtggtactg aacaacggac accccctggc cgtggaatgg atcgcccaac aggccgatgc
     1021 gctggtagag gcctgggaac cgggtatgaa gggaggcatt gctctggccg agatccttta
     1081 cggccttgtg aatcctacgg gcaaactgcc agtgaccttc ccccggtctg ccggcaacat
     1141 tctttcggtt tacaatcaca tgccttcaca ttacttccat cccatagtaa tggaagatcc
     1201 catgcctctc tatgaatttg gctatggtct gtcatacacc acgtaccgct atgacaacct
     1261 cacaccggga acattccttg acgggacact ggaagtgaag gtggatgtta caaacacagg
     1321 ttctgtggcc ggggaagaga ttgttttgct ttacgtgcgt gatgattatg cctccatgac
     1381 aaggcccgtg aaagaactga aagcgtttac gcgggttgcc cttgaaccgg gagaaacaaa
     1441 aacagtgagt ttgaaagtcg gccaggaaca gttgaagttc tggacccgag acagggggtg
     1501 gatcacagaa tccggtacat ttaccgttat ggcagggaac ctgtttgccc aggtcactct
     1561 gtcctgaagt cagccatgct ggtgatctgg tggactacat accggatcac cggtttaccg
     1621 gaacgttgca caaaaaatgt atccagggga acagtccctg tcatgttttc agggaatcgg
     1681 cttgattcaa tgtagtacag ttgttcaccg ggggctatcc cgcctctttt cctggtgatc
     1741 tgctcgtatt tatgggtctt ttccaatgga ccggttgttt ttacaaccgt ccctgctggg
     1801 cgcgccacat agtaatccag gtgggcaccc gggaaccagc gggtggccag gatggcactg
     1861 cccggtggca ttccccgtct gcccccgctt tccagaaaaa gtgcctcctg tttctctttc
     1921 acctgcgcaa aatgatctcc cagctgtgtc cagccgtaca tgtcaagggt aaagtcgtac
     1981 cgtcccagct ccttgtaggg atcttgcgga gtaatttcac ggggtggaac catttcacga
     2041 atgggaatga aaccggtctt gatctggaag actgacaaca ggaccactat agccagaatt
     2101 gccggggata atcctgcggg ccaacgcagg tctttgtgat ctgccacaga ggcagccagg
     2161 agcatcaggg gatagtatcc aggggcgctc cagtgcggca gggtctccct gaacagtgaa
     2221 aaaaacagga atacggctat cagcgggaca gactggaata tgatcatttg gaagatctcc
     2281 ggtcttactg gcagtgaaga ggtggtttta aggcgcttca gccgcaggta ataaaacaca
     2341 gaccaaatga caagcaacac gttcacagga ttgttgtaca ggattgatcc ggagatttcc
     2401 cttaagagat agaaaggttt gaaaccttca cccgaaatgg acaaccgtgc tgaatgaaag
     2461 gcaagactgg acatatcctg ggataaattc cacagcagca caggcatgaa caagagcagg
     2521 gaaatgccca aaccggccca taacctggac tctctgaaaa attttccccg gtatcgtaac
     2581 aagtacagga ttgctccggc ccagagaaaa atgcccgtgt atttactgag catggccagt
     2641 cccgtaaata cgccggccaa aaggaaaaat ccggagtttc tcttttggtc accgttttga
     2701 ccaagaccga aagccttgaa aaaacaatat aaggcagcca ggtaaaaagt gccgaggggg
     2761 gtatcgggca ggataaatgt ccctgccatg attgcagcgt atagcgaacc ggtgtaaagc
     2821 agcgccgcat accagccggt tttcgggcct ttgacaaggt atcccaggcg gtatatgagc
     2881 caggtgttaa atgtgcccag aacaagtgac ggcaggcgca gggcaaagga gctgtcccac
     2941 ttcaggttgc atgtaaatag ctggatgatc catcctacca tgggcggatg gtcaaaatga
     3001 ctccagtccg ggaacaaggc ataggtccag taatacacct catcgttacc cagctctgtc
     3061 agggctgcga ctatgcttct gaaaagcagt gagacagcca tcagcaggat tatccttttc
     3121 ccgtgatcct tcttcatctc aacaaagata ctaaagagca gggtattgtg tgcccgcatt
     3181 tttaatacct ttgtgaatat gaaagtagtt cttaccggag ccaatggcgg ggtgggcagg
     3241 atcatacaac agctggcccc cggacaaagc cggatggaat tggcagccta ttcaagacag
     3301 gaattgaata tacttgataa acaggctctt acggattgtc tatcgtctct gaagcctgaa
     3361 tatttaatta atacggcagc ctttacccat gttgaccggg ccgagaaaga acccgaagca
     3421 gcttttttgc tgaatgcagg ttgtctggaa ggaatggcag aggcctgccg gaaaacggga
     3481 acaatactca ttcacttgtc aaccgattac gtttttgacg gaaccaaaag aacaccttat
     3541 acagaagatg acatcccggc tcccgttaat acatacggag cctccaaaag ggcaggagag
     3601 ctcattgtcc tttcttattc ccgtggaata gtcatacgta ccagctgggt ctattcccgt
     3661 tattcccata attttcttgc tgccatcccc ctcttgctgc gaacccggtc agagcccttg
     3721 caggtagata cgctgcaggt gaattcgccc acgtatgctc ccgatttggt caatgcactg
     3781 tttaccctgg caagggaagg tataagccat ggattgttcc actttacaaa cacaggaggt
     3841 ggttgtaccc gttatgactt tgcctgtcat ctcagggaaa tgatcaacag gaaagacccc
     3901 ggggcagcat tggcacaggt cctgcccatg catgcagaag tacagggtgg tgcaccaaga
     3961 cctgtatata gtgttatgag tcccgctaaa attgctgcat tgacaggaat aaccattcct
     4021 cactggaagg aaggaattga aaatattata taaatcagat atggaaacaa gcattatgga
     4081 aaaagcccgg gaatggctcg accctttgta cgacgaacaa accagaaagg aagttagcag
     4141 actaatggaa gaagatccgg tattactgga agattcattt taccggaatc tggatttcgg
     4201 gaccggcgga ttgaggggca ttatgggcgt tggcaccaat cggatgaaca aatacaccgt
     4261 cgggatggtt acacagggac ttgccaatta catcaaaaaa acatatcccg aaccccataa
     4321 ggctgctatc tcttatgact gcaggaacca gtcacgggaa tttgctgaaa tcacagcccg
     4381 ggtgcttgct gccaatgatt ttacggttta tctttttgat gcactcaggc caaccccgga
     4441 gttaagcttt gccgtcagga aactggaatg ccacatgggg gtcatgataa ctgccagcca
     4501 taatccaaag gaatacaacg ggtataaagt gtacgggaac gacggggcgc aggtagtacc
     4561 tcctgatgat gagctgatca ttgaacaggt gagaaaagtc acctctcctg ccatggtcag
     4621 tttcacggga gaggggggaa agatcatctc actgggagaa gcgatggaca acgcctacct
     4681 tgattcgatt ctgagccttg tgaacctgag tccggatacg ataaataagt acacaaacat
     4741 gaaaatggtc tatacccccc tgcatggagc tggtgtccgt attgttccca tggccttgga
     4801 gaaaatgggt tttacccacg tgatccgtgt tccggaacag gaggtccctg acgggaattt
     4861 cccgactgtg gtatccccga acccggaaga gcccgaagcg ttgaaaatgg ctataaacaa
     4921 agctatggaa acaggagctc agctggtgat ggcgacagat cccgacactg atcgtatagg
     4981 gattgccgtt cgtaaccagg gtggggacat ggaattgttg aacgggaatc agacagcatc
     5041 cctgcttaca tactatctgc tcgaaaaatg gaaagagaca aaccgtctca agggcaaaga
     5101 atatatagtc aaaaccatag tcaccagcga cttgttgcag gtaatcgccg accattatgg
     5161 ggtctcatgc tataacgtgc tgacagggtt caagtatatt gcagaaataa tccgtaaaaa
     5221 cgaaggagaa aagatcttca tttgcggtgg agaggaaagt tacggattca atgtcggtga
     5281 gtttgtacgc gacaaagacg ctgtggtgac ctgtgtcctg gttgcagaag tcgcctgctg
     5341 ggcagctgca cgcggaaaaa ccctgcatga tctgcttaag gaaatgtacg ttcgctttgg
     5401 tttctttaag gaacggatgg tctcaaggac gctgaaaggc aaggatggca tggaacagat
     5461 taaagccatc atgaaaagct tcagggagaa tccgcctgag cagttgctgg gttcacctgt
     5521 tgttcttatg cacgattaca aaaaaagcga aacagtcgat ctggtcagtg acctgaggta
     5581 tcagatcaca cttccgaaat cagatgtgat ccagttcatc tgtgctgata atacggtcgt
     5641 tactgtcagg ccatcgggaa cagaacctaa gatcaaatac tattacgggg tgcagacaca
     5701 gctggtttca gcagcagctt tccgggaaac agaagtcctt ttggataaaa agctgacaga
     5761 cctgcatgcg tgttttcaaa attgattatg gtgccagaca agctttgcag gatccagtcc
     5821 aaagctttcc atcctttgaa ggatttcggc ccattgattc tctgtcaggt acttatcctt
     5881 tgaaaaaatc cagagctttg ttgctgaatt gttgcagaac aaggaggctg tataatccgg
     5941 atccaggtac agaatataac actttctgga caaattgacg aacagggtgg atttgaaaat
     6001 acccggattg tcagctttcc atttggctct tcccttgatt tcagtcaacg gcccgtatgg
     6061 agtgtatttg ttcttcttga tattcagacg gattataccg ttatcttcca ttgagacatc
     6121 cagggttacg gctgcaatct gtgagcgttc tggccttccc cattgagcca cttcatgcca
     6181 ggttccagta atacgctcaa tgtctatctc ttttaggacg ttttttcggg agacgaccgg
     6241 ttcttgcaag gtacatccgg tggccatgat caggatccag gtaaacagaa gcgacgcttt
     6301 atgattcatt tttttcatcg ttccttgtat tgatttcgga attcggttat ggttttccca
     6361 gagacacgtt taaaaaagcg gctgaaagaa gaaatctcct taaaaccaag gatatgtgca
     6421 atttctttgg cattcatgtt actatacagc aacaaccgtt ttgcttcggt ttctatgcgt
     6481 tcctggatga tttgcaaggg tgaaggcagc ttgtagagcg aaaagaggtt ggctatcgtt
     6541 tttggtgaac ggttaagcat ctgcgcataa tctccgactt ttttcttttc cttgaaattt
     6601 gaatcgacca ggatgataaa ctggcggaac aggtataagg cagactcgtg tgcaggttcg
     6661 atggcatact tttttcttgc tatcctggta taggtaatga tgaaccgttt aagatgaagg
     6721 cgaagcatct cttctttcag ggaatcatcc tctttgtatt caagccgtat atcagatacg
     6781 atactcccaa gagcatgtat ttggctgccc gtaagttgca tgggcagtgg attagcatat
     6841 cctgcaaaaa ggaatccatt acaggatact tcactatcag acttgaaaat gcaataaaaa
     6901 ttgctgttga aaaggatgga tatatatttg cccgatatac gggtaaagcg gatatggtgc
     6961 acattggata aaggaataac ctggtttttg accaggttaa tttccatgtg atctatagcc
     7021 agatccagcg atccctc
//
LOCUS       DAOSOF010000101         7013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10792_length_7013_cov_5.092555, whole genome shotgun sequence.
ACCESSION   DAOSOF010000101 DAOSOF010000000
VERSION     DAOSOF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10792_length_7013_cov_5.092555"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLM68_06420"
     CDS             <1..1016
                     /locus_tag="PLM68_06420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT55030.1"
                     /translation="MKVSIIIPVYNAAPTMERCLYSVLRQSYPDLEIILVDDHGTDNS
                     MVLATRLLEIPAGENAQPLATPGDHAAEAPVRSVIILHHNENRGLSAARNTGLEAATG
                     DYVFFLDSDDEIPIRAIENLIHTADLYSHPHLVCGEVDVKPDSKTVLKISNKLPAYIE
                     GNKASSRAILRREIPIMACNKLLRRDFLLSNKLYFKEGLLHEDNLWSFQLSGFVHNIA
                     VTRQVTYIYHINKKGLTMERVTPQRLNSIKTIITMQIESLSKERPLLTLQEAFIFFTA
                     AWFLDLLYEQSHQDYYSMRQWTRHALRPFSSVMHYHSLTNHMLYRAIYLPPAVLRAGK
                     KLR"
     gene            1064..3238
                     /locus_tag="PLM68_06425"
     CDS             1064..3238
                     /locus_tag="PLM68_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOT55031.1"
                     /translation="MFKNFFRTCFFFLVLLSQALPIRADEGMWLPMLIHQKIPQMKEL
                     GSRLAAEDIYSMNRACLKDAIVHFGGGCTGEVISAEGLLITNHHCGYGQIQSHSTVEH
                     DYLKDGFWARTRQEELPNKTLTVTFLRKMEDVTQQVLEGVTAQMSEVERQGLIDANIK
                     TISEQTLQGLDRKFYSVRITPAYYGNQYFLYLNETFRDVRLVGAPPSSIGKFGGDTDN
                     WMWPRHTGDFSLFRIYAGPDNQPADYSPDNVPYRPRHHLTISAKGVQPGDFTMVYGYP
                     GRTQQYVLSPAVDYLVNHSNPQKIRLRTLRLDVMNREQAKDPAVRIQYSSKNAGVSNS
                     WKKWQGETRGIINAGTIAKKQDFERRFQQWAVDSGRQEYINLIPAMKELYCQLEPYMF
                     ASEMYTEAVMAVELLRFASNLESTLVRNGTARAAEVARDFFKDYFLPIDREIFELLFT
                     EFYRLMEPIAHLLPGDAPEGQPLAAAGDFDQLTTATDFAQLAASLYNTSALTDRQKTM
                     ELLAKDSAQALSLIREDPAFRLVSPFVEKNRTAIQPQITEINRELNLLYRTWIQAIME
                     FDSDRIFYPDANSTLRVTYGTVQGYSPADAVMYLPVSTIDGIMQKDDPNIFDYDIPQS
                     LRDIYTVRDFGHWSSNGTVTVCFIATNHTSGGNSGSPVLNADGHLLGLNFDRVWEGTM
                     SDIEFDPSVCRNISVDIRYVLFVMEKIGKAEYLFKELDICKE"
     gene            complement(3655..3924)
                     /locus_tag="PLM68_06430"
     CDS             complement(3655..3924)
                     /locus_tag="PLM68_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOT55032.1"
                     /translation="MNIFVSSLSFKAKKEDLIELFGPFGEVVSARIILNKETRRSRGY
                     GFVEMANDEEALAAIKALNGTEHMGRTINVAVANERPAATPPQEI"
     gene            complement(3977..5422)
                     /locus_tag="PLM68_06435"
     CDS             complement(3977..5422)
                     /locus_tag="PLM68_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT55033.1"
                     /translation="MGGFFGIISKSPCANTLFYGTDYHSHMGTKRAGLATMNEQGIIK
                     RSIHRIENSYFRNKFEDELGEFSGNKGIGVISDNDPQPIVLRSHLGTFAIVTVGKINN
                     LEELGNELLSRGINFSELSSGQINPTEVVGHLITLEQSFADGIRHVQDKVRGSCSLLL
                     MTQDGIIAARDRYGRTPVVVGVRCGQEGTAIAFASESCSFPNLDLQLERFLGPGEAVM
                     AQAGSLEETDLNSSLSLKQLLPPREKLQICTFLWVYYGFPTSAYEGINVEEVRFRNGE
                     LMGKEEEYQVDSVCSIPDSGTGMAMGYAVGKGVPYRQAVAKYTPTWPRSFMPADKERR
                     ALVARMKLLPNADVIRGTRLVCCDDSIVRGTQLKNNVGVFLRNGVKEIHVRISCPPLL
                     FRCPYLHFTSARSDTELITRRIILAQKSDNLNPYIDPETPEHVAMVEGIRREMNFTSL
                     RFNSINNMITSIGLPKEKLCTYCFDGSGCGK"
     gene            complement(5448..>7013)
                     /locus_tag="PLM68_06440"
     CDS             complement(5448..>7013)
                     /locus_tag="PLM68_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT55034.1"
                     /translation="RYGLIVYYAGLVVLCCCAFWNGLFQYATIGPAPGRVKAGERNIL
                     CLRVKEFPVPVVTASGRHYERMECRLVIYSPDGRRQIQGNENMLVYVAVDNMRKGYRQ
                     DTMAAKPTGLLPGDVFMTRSRTFSFAGDSPAGASASAASSFDYPRYMARRGFYCRTYV
                     YDYRRIQSELTLYERLRRLRVPLTAHWEGEAGAILSGICLGDKSGLGKDVRERFNVAG
                     ASHILAVSGLHVGALYGSLVFVLGLPGRIREERRYKRRRRITVSAVPEYGQIAAARSQ
                     ILKTYSLSGGAWAHVVALLLIWSYAAVVGFSASVMRAALMLTIYGAGRVLGTRTHGLN
                     VLSLAALILVIAKPMNLYDVGFQLSFIAVLSLMLFFPLFRNLLSVRNAIWKYLWELFC
                     CSLSVQLGTTWLVAGTFGVIPLYGLLCNFFVVPFSAVILYGFLLYLVAMGVHAVAGLW
                     GLPVLTLENVLKTVNLLLHYLAGILDKAVSLFSGLPYTPIRYQPDFIEQFLMLWCTGY
                     LYFFLREREELKG"
BASE COUNT         1862 a         1952 c         1573 g         1626 t
ORIGIN      
        1 ccatgaaagt atcaatcatc attcccgtat acaatgcggc acccaccatg gaacgatgcc
       61 tctattcggt cctcaggcaa agctatccgg acctggagat catactggtg gacgaccacg
      121 ggacggataa cagcatggta ctggccacac gcctgctgga aatccctgcc ggcgaaaacg
      181 cccagccact tgctactccc ggagatcacg ctgcagaggc accggtccgg tctgtgataa
      241 tattgcacca caatgaaaac cggggtcttt ccgcagcccg gaacacaggg cttgaggccg
      301 ccaccggtga ctatgtgttc tttctggaca gtgacgacga aatccccata cgtgccatag
      361 aaaacctaat ccatacggct gacctctaca gccatccgca cctggtatgc ggagaggttg
      421 atgttaaacc cgacagcaaa accgtactga aaatcagtaa caaactaccc gcctacatag
      481 aaggcaacaa agcctcatcc cgggcaatcc tccgcaggga gatccccatt atggcctgca
      541 ataagctgtt aaggcgcgat ttcctgctct ccaacaaatt gtatttcaag gaaggactcc
      601 tacacgagga caacctgtgg agtttccagc tctcgggatt tgttcacaat attgccgtca
      661 caagacaggt aacctatata taccacatta ataaaaaggg gcttaccatg gaacgtgtga
      721 ccccccagcg cctgaacagt atcaagacca tcatcaccat gcagatagaa agcctgtcaa
      781 aggaacgccc gctgctgaca ttgcaggaag cgttcatctt tttcacggcg gcctggttcc
      841 tggacctgct ctacgaacag tcccaccagg attactacag catgagacaa tggacccgcc
      901 atgcattgcg tcctttcagt tccgtcatgc attaccactc cctgaccaac cacatgcttt
      961 acagggccat ttatttgccc cctgcagtgc tgcgtgctgg aaaaaaactt cggtaaaatg
     1021 catcccaacc acgataatat cacccaataa acagtgaatc aatatgttca agaatttttt
     1081 ccgcacttgc tttttctttc tggtgctgct ctcccaggca cttcccattc gtgccgatga
     1141 aggcatgtgg cttcccatgc tcattcatca aaaaatcccg caaatgaagg aactcggttc
     1201 ccgtcttgcg gcagaggaca tttacagcat gaaccgagcc tgtcttaaag atgcgatcgt
     1261 gcatttcgga ggcggatgca ccggggaggt catctcggca gagggattgc tcataacaaa
     1321 ccaccattgc ggttacggac agatacagtc ccacagcacg gtggaacatg attacctgaa
     1381 agacggtttc tgggcccgta ccaggcagga ggaactgccc aacaaaaccc tcacagtcac
     1441 attcctgcgt aaaatggaag atgtgacgca acaagtgctc gaaggggtga ccgcccaaat
     1501 gagcgaagtg gaacgccagg gtctgataga tgccaacatc aaaaccatca gcgaacaaac
     1561 actccagggc ctggaccgca aattctactc ggtccggatt actcccgcct attacggaaa
     1621 ccaatatttc ctgtacctta acgagacatt ccgcgacgtg cgtctggtgg gtgccccgcc
     1681 ctcttccata ggcaaattcg gcggcgacac agacaattgg atgtggccgc gccatacggg
     1741 cgatttttca ctgttccgca tttatgcagg tcccgacaac cagccggcgg attacagtcc
     1801 cgacaacgtg ccttaccgtc cgcgccacca cctgaccatt tcggctaaag gcgtgcaacc
     1861 gggcgatttc accatggttt acggctatcc cggacgcaca cagcaatacg tgctctctcc
     1921 cgctgttgac tacctggtca accactccaa cccgcaaaag atccgcctgc gcacccttcg
     1981 tctggatgtc atgaacagag aacaggccaa agatcccgcg gtccgcatcc agtattccag
     2041 taaaaatgca ggggtatcca attcctggaa aaagtggcag ggcgaaaccc ggggtatcat
     2101 caatgccggc accatagcca aaaaacagga ctttgaacgt cgtttccagc agtgggcagt
     2161 ggactcgggc cgtcaggaat acatcaacct gatccccgcc atgaaagaac tttattgcca
     2221 gctggaacct tatatgtttg cttccgagat gtataccgag gcggtcatgg ccgtggaact
     2281 gctgcgtttt gccagcaacc tggaaagcac ccttgtgcgc aacggaaccg cccgcgcagc
     2341 cgaagtcgcc cgtgatttct tcaaggatta cttcctgcca atagaccggg aaatctttga
     2401 gctgctattc accgaattct accgcctcat ggaacccatc gcccacctgc ttcccggcga
     2461 tgcacctgaa ggccagcctc ttgctgctgc cggggacttc gaccagctga ccactgccac
     2521 agacttcgca cagctggctg ccagcctgta caacacctcg gctttgaccg accgtcaaaa
     2581 aaccatggag ctgctggcca aagactctgc acaagccctg agcctgatcc gcgaggatcc
     2641 tgccttccgc ctggtcagcc cctttgtgga aaagaaccgt actgccatcc agccgcagat
     2701 cactgagatc aaccgtgagc tgaaccttct gtatcgtacc tggattcagg ccatcatgga
     2761 atttgacagc gaccggatct tctaccccga tgccaattcc acccttcgtg tcacgtatgg
     2821 aacggtgcag ggatacagtc ccgcagacgc cgtcatgtat ctgcctgtca gcaccataga
     2881 tgggatcatg caaaaagacg acccaaacat cttcgactac gacattcctc aatccctgcg
     2941 ggacatatat actgtacggg attttggaca ctggtcctca aacggaaccg ttactgtatg
     3001 tttcatagct acaaaccaca ccagtggcgg aaattccggt tcgcctgtcc tcaatgcgga
     3061 cgggcacctg ctgggtctga attttgaccg tgtatgggag ggtaccatga gcgacattga
     3121 atttgacccg tctgtttgcc gcaatatatc tgtagatatc cgttatgtgc tttttgtgat
     3181 ggaaaagatc ggaaaagcgg aatatttgtt caaagagttg gatatttgca aagaatagtt
     3241 aattttgttc cgtaattaac accttagtac tatggatcaa attattgaaa tcatcaccaa
     3301 cctctttgct caaattgcag agtggttcca ggctttcatc caatggatca tgagcatttt
     3361 taccgtataa ttacattatc gattcgaatg ttattttcct taaaaagcca tccttcggga
     3421 tggctttttt tatcagagta tgcagctatt gtattttttt ttgtatcttt acattaaatc
     3481 acactaacac tacaatagaa tttttattat ggactttggt gaagtaatca tgtggctgtc
     3541 gctggtcata tcaatcctga cagcaatcct gggaatactc cagttgtttg ttcccgctac
     3601 ttaaaaaaaa agctgacttt aaggtcagct ttttttacaa cttcagagat atagctatat
     3661 ttcctggggc ggggtagctg caggacgttc attggcaact gccacattta tggttctgcc
     3721 catatgttca gtaccattca acgcttttat agctgcaaga gcttcttcat cattggccat
     3781 ctcaacaaag ccataaccac gggaacgacg ggtctccttg ttaaggataa tgcgggctga
     3841 tacaacttct ccaaaaggac cgaaaagttc aatcaggtct tctttttttg ccttgaaact
     3901 aaggctggaa acaaaaatgt tcatggaata agggatgatt tgttaggtta aatgtaattc
     3961 aaatataaca ttattttcat ttcccgcaac ctgacccgtc aaaacagtac gtacataatt
     4021 tttccttggg caagccaata gaagtaatca tgttgtttat actgttgaat ctcaacgatg
     4081 taaagttcat ttcccggcgg attccttcta ccattgccac atgctccggt gtttccggat
     4141 caatgtaagg attcagattg tcagacttct gagccaaaat gatacgacgt gttatcagtt
     4201 ccgtatcgga acgggcagac gtgaaatgca ggtacggaca cctaaagagg agtggcggac
     4261 acgagatccg tacatggatc tccttcaccc cgttgcgaag aaagacaccc acattgtttt
     4321 tcaattgtgt acccctgaca atggagtcgt cacagcaaac aagccgcgta ccgcgaatga
     4381 catcggcatt gggcaataat ttcatcctgg ccaccagggc ccgtcgctcc ttatcggccg
     4441 gcataaaact ccggggccat gtgggggtat atttcgcgac agcctgcctg taaggcactc
     4501 ccttccccac tgcgtaaccc atggccatcc ccgtaccgct atccggaatg ctgcaaaccg
     4561 agtccacctg gtattcttcc tcctttccca tcagctcgcc gttacggaaa cgcacttcct
     4621 ccacattgat cccttcatag gccgaggtgg gaaatccgta atacacccag agaaaagtgc
     4681 agatctgcaa cttttcgcgg ggcggaagca gctgcttcag tgaaagagaa gagttcaagt
     4741 cagtttcttc cagagaaccg gcctgcgcca tgacggcttc acctggtccc aggaacctct
     4801 ccagctgcag gtccaggttc ggaaaactgc agctttcact ggcaaaggca atggcagtgc
     4861 cctcctggcc acagcggact ccaacgacaa caggagtgcg cccgtaacgg tcccttgcgg
     4921 caatgatccc atcctgtgtc atgagcagca agctgcaact tccgcgcacc ttatcctgca
     4981 catgacggat cccgtcggca aaagactgtt ccagggttat caggtgtccc accacctcgg
     5041 tgggattgat ctgaccagaa ctgagttcag aaaaattaat acccctggaa agcaactcgt
     5101 tgcccaattc ttccaggttg tttatttttc cgacggtaac aatggcaaat gttccaagat
     5161 gagagcgcaa tactatgggc tggggatcgt tatcgcttat gacaccaatt cctttgttgc
     5221 ccgaaaattc tcctaattcg tcttcaaact tgttcctgaa ataactgttc tcaatcctgt
     5281 ggatgctccg cttgatgatg ccctgctcgt tcatggttgc aagtcccgcc ctctttgtac
     5341 ccatatggga atggtaatca gtaccataaa aaagagtgtt agcgcaaggc gatttagata
     5401 tgattccaaa aaaacctccc atagctgcaa agataaacaa atctggttca ccccttcaat
     5461 tcttcccttt cacgcaggaa aaaatacagg taacccgtac accatagcat caggaactgt
     5521 tcaatgaaat cgggctggta acgtatgggg gtatacggaa gaccggagaa aagcgatacg
     5581 gccttatcca gtatcccggc caggtagtgc aacagaaggt tcaccgtttt cagaacgttt
     5641 tccaaagtca gaacgggcaa tccccacaat ccggccacgg catgcacacc catggccacc
     5701 aggtacaata aaaagccgta caggatcact gcactgaaag gaacgacaaa aaaattacaa
     5761 agcaagccgt atagcggaat cacgccaaag gtacctgcca ccagccaggt cgtgcccagc
     5821 tgcactgaaa gcgaacaaca gaacaattcc cacaaatatt tccatatcgc attacgcaca
     5881 ctcaacaggt tacggaacaa aggaaaaaac aacatcaggc tcagaacggc gataaaggaa
     5941 agctgaaagc cgacgtcata aaggttcatt ggctttgcta tcaccaggat caaggcagcc
     6001 aatgaaagaa cgttcagtcc gtgtgtcctg gtcccgagca ccctgcctgc accatagatg
     6061 gtcaacatca gggcagcgcg catcacgctg gcactaaatc ccaccacggc agcatacgac
     6121 cagatcagaa gcaaggctac cacgtgcgcc caggcgcccc cggaaagcga atacgttttc
     6181 aggatttgcg atcgtgcggc agctatctgc ccgtattccg gaaccgcaga caccgttatc
     6241 cttcgccttc ttttgtaccg tcgttcctcc ctgatcctgc cgggcaagcc tagcacaaag
     6301 accaaactcc catagagtgc tcccacgtgc aaaccactga cggccagaat atggctggca
     6361 cccgccacat tgaaccgttc gcgtacatcc ttgcccagtc ctgacttatc cccaaggcat
     6421 atccccgaca agatagcacc cgcctcacct tcccaatgag cggtaagcgg gacacgcaac
     6481 ctcctgagcc tttcatataa ggtcagttct gattggatcc tgcgatagtc atatacatac
     6541 gtcctgcaat aaaatcctct gcgcgccata taccgtggat aatcaaaaga cgaggcagca
     6601 gaagcagatg cgccggccgg tgaatcacct gcaaaagaaa acgtccggct tcttgtcatg
     6661 aatacgtctc cgggcaacaa tcctgtgggc tttgcggcca tggtgtcctg ccggtaacct
     6721 ttgcgcatgt tgtcaacagc aacatagacc agcatgtttt cattaccctg gatttgcctt
     6781 cttccatcgg gcgaatatat gacaaggcgg cactccatgc gttcgtaatg ccttcccgag
     6841 gcagtaacta caggaacagg aaattccttt accctgaggc ataaaatgtt cctttcaccc
     6901 gcttttaccc gtcctggagc aggtcctatg gttgcatact ggaacaagcc attccagaaa
     6961 gcacagcagc acaataccac cagacctgca tagtacacga taaggccgta ccg
//
LOCUS       DAOSOF010000102         6997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10820_length_6997_cov_5.202103, whole genome shotgun sequence.
ACCESSION   DAOSOF010000102 DAOSOF010000000
VERSION     DAOSOF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10820_length_6997_cov_5.202103"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLM68_06445"
     CDS             <1..860
                     /locus_tag="PLM68_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT55035.1"
                     /translation="DIQDIGSRSFTYISTMGLAMEACAELGIEFVVLDRPNPIGGRKV
                     EGCYVEDGCFTFVSQFRIPYVYGLTCGELALLLNGERMLGSGARPGETAQCNLRVIPM
                     EGWNRDMFYDQTGLQWIASSPHIPQAITAFFYPMSGIAGDLSVVSIGVGYTLPFQLFA
                     PADSTIVPEELARNLNNLQLPGLHFRPIYFRPFYSRGQGTTLGGVQAHLTNPSAAPLT
                     DVNFYVIQEIHKLNPGIVFNLERITKVVGSPFITAKFAERYQFSDIRDYWYKDAEAFR
                     QLSQRYYLY"
     gene            872..1999
                     /locus_tag="PLM68_06450"
     CDS             872..1999
                     /locus_tag="PLM68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT55036.1"
                     /translation="MKRFCYLLFFLLVLSCSREKITPEPEPEEEKPEEFVPAARIEGR
                     VALSYVTYYSQGIPDPTLLTHICYAFAELYVVNGQYREFRLQGDESRFAAVVDLKKNH
                     PGLKILLAFTHTIVNKDNAQEGGFSDMSSTEEGRVAFAQDCLEFIQIWGIDGIDIGWE
                     FPGISWSGHKSNKAADTQNHVLLMKQLRQTLGPDLLITFPGYVMNAMEVSGGTRYIDL
                     KGVEPYVDFVNVMTYDMDEAPHHHSALEDSRAYWDCARTLQAYRAAGFPTEKMVLGIP
                     FYGRISFSKSPSAITYRQIKNLDPSLYSIDNWDPTASVPYVSRISTGAFYCGYDNAAS
                     IAQKGSWALSQGMKGLMCWMYNGDDDNGTLRKAMWNAVMKP"
     gene            2043..3458
                     /locus_tag="PLM68_06455"
     CDS             2043..3458
                     /locus_tag="PLM68_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT55037.1"
                     /translation="MKQLETLFKSYTGFPARSITELPSSGSNRRYFRMEGKQGTLIGT
                     IGSCKEENKAFIAIAEHFHKQGLPVPEVFAHSRDFSCYLQKDLGDRILYNAVEEGRSR
                     GKYNTAEKDLLFKAMNALPALQFKGAEGLDFDVCYPQPEFDERMISFDLNYFKYCFLK
                     ATGLDFSEIKLENDFRKMSAVLLRDPSETFLYRDFQARNVMLVNEEPYFIDFQGGRKG
                     PVYYDVASFIWQAKANYSEELKEELLSAYLKALKQYIPVDEKHFHRQLRHFILFRTLQ
                     VLGAYGFRGYFEKKPHFLQSVPYAIDNLRKLLKEPFTEYPYLSSLLLELTKMRQYADM
                     DKERKLQVTVCSFAYKKGIPNDVSGNGGGYVFDCRGLENPGKFEHFRHFTGEDQEVIS
                     FMEEDGGVKGFLEHAYVLMDTHVQRYIERKFIHLMCCFGCTGGQHRSVYCARHMADYL
                     AKKYNIRVHLYHRELDLEIDY"
     gene            3466..4206
                     /locus_tag="PLM68_06460"
     CDS             3466..4206
                     /locus_tag="PLM68_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT55038.1"
                     /translation="MQAMILAAGLGTRLKPITDKLPKALVPVRLQDGSHKPLLELLLD
                     KMSEQGVNRFVINVHHLAGQIRTFIKDYLERKGLPIQIAVSDESQLLLDTGGAIREAR
                     SLLDHAEPFLVHNVDILSNLHLDDLYSRHKESDLATLLVSQRDTPRQLLFDKQWHLVG
                     WTNTQKGEFRWVREAYPMKDCMMRAFAGIHIMSPLVMPLMDPWPRIFSVIDFYLETAR
                     TEVVRGIEIPGLEITDIGKPGTLENLIL"
     gene            4203..5384
                     /locus_tag="PLM68_06465"
     CDS             4203..5384
                     /locus_tag="PLM68_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOT55039.1"
                     /translation="MKYDFDTIIDRRGTDTVKIGRLKELFGKEDLIPLWVADMDFETP
                     PFIRKALEDRFRHPIFGYTIAFEDYWQSIISWLKYLHGWEIRREWLTYIPGIVKGIGL
                     AIVHFTGKGDHVIIQPPVYHPFRMVTERNGRVVQNNPLIWDGTRYQMDLDHMEDVLKR
                     RRCPLLVLSNPHNPGGCMWSKETLADLAHICYKYNVTVISDEIHADMPMFGNRHTPFT
                     SVSQEAAQVGIVFGAPSKTFNMAGLVSSFAVIENPQLRKAFYGFLHACELDDPTLIAT
                     IATHAAYSQGKEWHRQMLDYVENNINYVVTQLPRLIPLIKAPRPEASFLVWLDCRDLK
                     KAGIEDIPGFFTGAGLALNDGSMFGPGGEGFMRLNVGCPKIILEKALEQLYDHYRSII
                     C"
     gene            complement(5374..6108)
                     /locus_tag="PLM68_06470"
     CDS             complement(5374..6108)
                     /locus_tag="PLM68_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT55040.1"
                     /translation="MKRAVIMGATSGIGREVALLLLSEGWHVGIAGRREERLKQIQAL
                     QPQSIAYEVIDVTREDAPVLLQHLIDKLGGMDLYLHCSGNGRYNPDLDPSLEKEVVRT
                     NVNGFINMTNHAFRYFKEQKGGHLAAVTSVAGRRGIALNALYSSSKRFQMTYLTALAQ
                     LSHNGKYGITITDIQPGFVKTDFLHHSYPLMMKTPYVARKIVQGLRKGKRRVVIDWRY
                     RVIVCVWHMIPGWIWERINLQRFVSR"
     gene            complement(6112..>6997)
                     /locus_tag="PLM68_06475"
     CDS             complement(6112..>6997)
                     /locus_tag="PLM68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT55041.1"
                     /translation="VVVLSTLADSRKRHLFKAVAHHKHIVTEKPLGSTIQEEYDVLEK
                     ISGNSILVTVNLPLRTAWYHKTIREFIDSGEIGDLAIVRICHMTPGLAPGEGHWAEGP
                     LFHDCGLHYVDIAHWYAQSDFKTWNAQAIRMWEYHEPWWLQAHGTFENGIVFDITQGH
                     VYGQLAKDLIHNGYVDLIGTKGIARMTHDFVTATVELHGITKTQTIRKPFGDKNLDIL
                     LDLFAESVIRGTLDPSLPKVKDAVIASEYAWKFLEDAARHDMPVRGDSKTLEEIRERR
                     NNKRIGYGLLRKKNKFNE"
BASE COUNT         1775 a         1693 c         1728 g         1801 t
ORIGIN      
        1 acgatatcca ggacatcggc tcgcgttcct tcacctacat ttccaccatg ggacttgcca
       61 tggaggcctg tgcggaactg ggcattgaat ttgtggtcct ggaccggccc aatcccattg
      121 ggggcaggaa ggtcgagggg tgttacgtgg aagacggctg ctttaccttt gtcagccagt
      181 tcagaatacc ctatgtatat ggtcttacct gcggtgagct ggcgttgctg ctcaatggag
      241 agcgcatgct gggatccgga gcccggcccg gggaaactgc ccaatgcaac ctgcgcgtaa
      301 ttcccatgga aggatggaac cgcgacatgt tctatgatca gacaggatta cagtggatag
      361 cctcttcccc gcatattccc caggccatca ccgccttctt ctatcccatg agcggcattg
      421 caggggattt atccgtggtc tccatagggg tcggctatac gcttccattt cagttgtttg
      481 caccggcaga cagcaccatt gtacctgaag aactggcccg caatctgaac aaccttcagc
      541 tgcccggcct tcatttcaga cccatttatt tccggccttt ttacagcagg ggacaaggaa
      601 ctactttggg cggggttcag gcacatctga caaatccgtc ggcagccccg ctgaccgatg
      661 tgaattttta cgtgatccag gaaatccaca aactcaatcc gggtattgtc tttaacctgg
      721 aaaggatcac caaagttgtg ggaagtcctt ttataacagc aaaatttgca gaacgttacc
      781 agttcagtga tatcagggat tactggtaca aagacgccga agcattccga cagctgtcac
      841 aacgctatta tctttattga ttataaaacc tatgaaacgg ttttgttacc tgttgttctt
      901 cctactggtc ttgtcgtgca gtcgtgaaaa gattaccccg gaacccgaac cggaagaaga
      961 aaaaccagaa gagtttgttc cggccgcacg aatagaaggg cgggtggctt tatcctatgt
     1021 aacatactac agccagggga tcccggatcc cacgctgttg acacatattt gttacgcctt
     1081 tgccgagttg tatgtggtga acggccagta ccgggaattc cgcctgcagg gcgatgaatc
     1141 ccgttttgct gcagtggttg atctgaaaaa aaaccacccc ggccttaaaa tcctgcttgc
     1201 cttcacccat accattgtca ataaggacaa cgcccaggag ggcggcttct ctgacatgag
     1261 cagtacagag gaaggcaggg tggcttttgc acaggattgc ctggaattta tccagatctg
     1321 ggggattgac ggaattgata taggatggga atttccggga atttcctgga gcggtcataa
     1381 aagcaataag gctgccgaca cgcaaaatca tgttcttttg atgaaacagc tgcgccagac
     1441 actggggcct gacctgctca tcactttccc gggttatgta atgaacgcta tggaagtcag
     1501 cgggggaacc cgctatattg acctgaaagg ggtagaaccc tatgtggact ttgtcaatgt
     1561 gatgacgtat gatatggacg aggcccctca tcaccattcg gcactggagg attcacgggc
     1621 ttactgggat tgtgcacgga ccctgcaggc ttacagggca gccggttttc caacggaaaa
     1681 gatggtgctc ggcattcctt tttatggaag gatttccttt tccaaaagcc cgtctgccat
     1741 cacataccgg caaataaaga atctggatcc atccttgtat tcgattgata attgggatcc
     1801 cacagcttct gtgccttacg tcagccggat cagtacagga gcgttttact gcgggtatga
     1861 caatgctgcc agcattgcac aaaaaggatc ctgggcactg tcacaaggca tgaaaggact
     1921 tatgtgctgg atgtataacg gggacgatga taacggcact ttaaggaagg ccatgtggaa
     1981 tgccgtaatg aaaccataga gattccctat ctttatagaa attttagcta tttttgtgcc
     2041 atatgaaaca actcgaaact cttttcaagt catataccgg ttttcccgca cgttccatta
     2101 ccgagttgcc ctcatccggg agcaacaggc ggtatttcag aatggaagga aaacagggca
     2161 ctctcatcgg cacgataggt tcatgcaaag aggaaaacaa ggcttttata gccatagcag
     2221 aacatttcca taaacagggg cttcctgtcc ccgaggtttt cgcccatagc cgcgattttt
     2281 cctgctacct gcagaaggac ctgggagaca ggatcctgta caatgccgtg gaagaagggc
     2341 gctccagggg aaaatacaac actgctgaaa aagatttgct tttcaaagcc atgaatgctt
     2401 tgcctgccct acagtttaaa ggagcagaag gacttgattt tgacgtttgt tatccgcaac
     2461 cggaatttga tgaaaggatg atctctttcg atctgaatta tttcaaatac tgctttttaa
     2521 aggcaacagg cttggatttc agtgaaatta agctggaaaa cgacttcagg aaaatgtctg
     2581 ctgtcctgtt gcgggatccg tcggagacat tcctgtacag ggatttccag gccaggaatg
     2641 tcatgctggt caacgaagaa ccctatttta tcgatttcca gggaggtagg aaaggcccgg
     2701 tttattacga cgtggcttct ttcatatggc aggccaaagc caattattcg gaagaactca
     2761 aggaagaact tctctcggcg tacctcaagg ctttaaaaca atatattcct gtggatgaaa
     2821 aacacttcca caggcaattg cgtcatttta tcctgttccg caccttacag gtactcggcg
     2881 cgtatggatt ccggggttat tttgagaaaa aaccccattt cctgcaaagt gtgccctatg
     2941 ccatagacaa cttgcgcaaa ctgcttaaag aaccctttac cgagtatccc tatttgtctt
     3001 ccctgttact ggaactgacg aaaatgcgcc agtatgcgga tatggataaa gagcgcaaac
     3061 tccaggtcac tgtctgcagt tttgcctata aaaaaggcat tcccaacgat gtaagcggta
     3121 acggcggagg ttatgtgttc gattgccgcg gcctggaaaa tccgggaaaa tttgagcatt
     3181 tccggcactt taccggtgaa gaccaggagg tcatcagctt catggaagaa gacggtgggg
     3241 tgaaaggttt cctggaacac gcctatgttt tgatggatac tcatgtacaa cgttatattg
     3301 aaagaaaatt catccacctg atgtgttgct tcggatgtac cgggggacag caccgttctg
     3361 tctattgtgc acgccatatg gccgattacc tggctaaaaa atataacatc agggtacatt
     3421 tgtatcatcg tgaactggat ttggaaatag attactagac agaccatgca ggcaatgatt
     3481 ctcgctgcgg gtctcggtac ccggttgaaa cccattacag ataagctccc caaagccctt
     3541 gtcccggtaa ggttgcagga cgggagccat aagccattgc ttgaattatt gctggacaag
     3601 atgagtgaac aaggcgtaaa ccgctttgtg atcaatgtgc atcatcttgc cggtcagata
     3661 agaactttca ttaaagatta cctggaaaga aaagggctac ccattcagat tgcagtttcc
     3721 gatgagagcc agctgctgct tgacaccggg ggagccatcc gtgaagcccg ttcgctgctg
     3781 gatcatgctg aacctttcct ggtacacaat gtggatattc tgtcaaacct gcacctcgac
     3841 gacctttata gccgtcataa ggagtctgac ttggcaacct tgctggtcag tcaaagggat
     3901 acaccgcgtc agctgctctt tgacaagcaa tggcacctgg tggggtggac aaatacccag
     3961 aaaggagaat tccgctgggt tcgcgaggct taccctatga aagactgtat gatgcgtgct
     4021 ttcgccggaa ttcatatcat gtcccccctg gtaatgcccc tgatggaccc gtggccccgg
     4081 attttttcag tgatcgactt ttaccttgaa accgccagga ctgaggtggt acgcgggata
     4141 gaaatccccg gactggagat tactgacatc ggaaaacccg gcacactgga aaatttgata
     4201 ctatgaaata cgattttgac accattattg acagaagagg aacagatacc gtaaagatcg
     4261 ggcgtttgaa agagcttttt ggaaaagaag acctgatccc cttatgggtg gccgatatgg
     4321 actttgaaac gccgcctttt ataagaaaag ccttggaaga cagattcaga cacccgattt
     4381 tcgggtatac gatagctttt gaagattact ggcaatctat catatcttgg ttgaaatacc
     4441 tgcatggctg ggaaatacgc cgcgagtggc taacctacat tccgggaata gtcaagggta
     4501 taggactggc aatcgtacat tttacaggga aaggcgatca tgtgatcata caacccccgg
     4561 tttaccatcc tttccgtatg gtaaccgaac gaaacggaag ggtagtacaa aacaatccat
     4621 tgatctggga cggtacccgt taccagatgg acctggatca catggaagac gttctgaaac
     4681 gccgcaggtg ccccctgttg gtgctctcta acccccacaa tccgggaggc tgcatgtgga
     4741 gcaaggaaac ccttgccgat ttggctcata tctgttataa atacaatgtt accgtgatct
     4801 ctgacgagat ccatgccgac atgcctatgt tcgggaaccg ccacacaccc ttcacgtctg
     4861 tttcccagga ggcggcgcag gtaggcattg tgttcggggc tccatcaaaa acctttaata
     4921 tggcgggact ggtcagttct tttgctgtca tagagaatcc acagctcagg aaagctttct
     4981 atggattttt acatgcctgc gaactggacg atcccacctt gatagccact attgccacgc
     5041 atgctgctta tagccaggga aaggaatggc acagacagat gcttgattac gtggaaaata
     5101 acataaatta tgtggtgacc cagctacccc gcctgattcc cctgatcaag gcaccccgtc
     5161 ccgaggcctc tttccttgta tggctggatt gcagggatct gaaaaaagca ggtatagaag
     5221 acattcccgg cttttttacc ggtgcaggac tggctctgaa tgacggttcc atgtttggcc
     5281 cggggggaga gggttttatg cggctgaacg taggctgccc taagataatt cttgagaaag
     5341 cgctggaaca gttatacgac cattacagga gcattatctg ctgacaaaac gctgcaggtt
     5401 tatcctttcc catatccaac cgggtatcat gtgccagacg catactatga cacggtaacg
     5461 ccagtcaatg acaaccctgc gtttcccttt acgtaacccc tgtacgatct tgcgtgccac
     5521 ataaggggtt ttcatcatta gcgggtaaga atgatgcaga aagtctgtct tgacaaatcc
     5581 aggctggatg tctgtgattg taattccata tttcccatta tgggacaatt gggccagtgc
     5641 agtaaggtaa gtcatctgga agcgttttga agacgaatac aaggcgttca gagctatgcc
     5701 ccgcctgcct gcaacagaag tcacggcagc caggtgtccg cctttctgct ctttaaaata
     5761 gcggaaagca tgatttgtca tattgataaa tccgttgaca ttggtcctga caacctcttt
     5821 ttccagcgaa ggatcaagat ccgggttgta cctgccgtta cctgaacaat gcaggtacag
     5881 gtccatgcct cccagcttgt caataagatg ctgaagcaat acaggggcat cttcccgggt
     5941 cacatctatc acttcatatg ctatggactg aggttgaaga gcctgaattt gctttagcct
     6001 ctcttcccgt cttccggcaa tgccaacatg ccagccttca ctgagaagga gaagggctac
     6061 ttcccgtcca atgcccgatg tggctcccat gatgacagcg cgcttcatac ctcattcatt
     6121 gaatttgttc ttttttctca acagcccgta gccgatacgt ttgttattcc tcctttccct
     6181 gatttcttca agtgtcttgc tgtccccccg gacgggcata tcgtgccggg ctgcgtcctc
     6241 caggaatttc catgcatatt ccgaggcaat gacggcatct ttcaccttag gaagggaagg
     6301 gtcaagggtt ccccggatga cagattcggc aaaaaggtcc agtaagatat ccaggttctt
     6361 gtctccgaaa ggtttccgga ttgtctgtgt tttggtgatt ccgtgcagtt ccaccgtggc
     6421 tgttacaaaa tcatgggtca ttcttgcaat gcccttggta ccgatcaaat caacataccc
     6481 gttgtggata aggtcctttg caagctgtcc gtatacatgc ccctgggtta tgtcaaagac
     6541 aatgccgttt tcaaaggttc cgtgagcctg cagccaccat ggctcatgat attcccacat
     6601 gcggatggcc tgtgcgttcc aggtcttgaa atcactttgg gcataccagt gtgcaatatc
     6661 cacataatgc aagccgcaat cgtgaaagag cggtccttct gcccagtgtc cttctccggg
     6721 tgctaatccc ggcgtcatat gacatatcct gacaatggca aggtctccga tttccccgct
     6781 atcgatgaat tcccggatgg tcttgtgata ccaggctgtc ctgagcggca ggttgaccgt
     6841 taccaggata ctgttcccgc tgattttttc cagcacgtca tattcttcct ggatggttga
     6901 accaagcggt ttttcggtaa caatgtgctt atggtgcgcc acagccttga aaagatgcct
     6961 tttgcgcgaa tcggcaaggg tggacaaaac cacaacg
//
LOCUS       DAOSOF010000103         6969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10888_length_6969_cov_5.375615, whole genome shotgun sequence.
ACCESSION   DAOSOF010000103 DAOSOF010000000
VERSION     DAOSOF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10888_length_6969_cov_5.375615"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLM68_06480"
     CDS             complement(<1..845)
                     /locus_tag="PLM68_06480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT55042.1"
                     /translation="MKKHFLLTLLLFPALLGAQDFAPYFQQVAKEWNITGSALAVIKN
                     DSIVFVQGFGEKRNGSGLPVDGNTVFQIGSVSKSFTATLMAMLVDDGLVNWNDPVKWY
                     LPDFALYDPWVTDNLEVRDLFLHRSGLPGQAGTYIPCLGYGRDDVYRMLRFIPPANSV
                     RTTYGYNNVMFIVAQKIIEAVTGKSWEDNLQERIFTPLGMHNTSTNEDGFLATANGNT
                     PHETTVRNGKIHTAPMEGEEQALHWLTVIGAAGGINSTVTDMMQWCRLHLSNGVVDGD
                     TLLSAA"
     gene            879..2252
                     /gene="radA"
                     /locus_tag="PLM68_06485"
     CDS             879..2252
                     /gene="radA"
                     /locus_tag="PLM68_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT55043.1"
                     /translation="MSKVKKAWFCQNCGAESSKWLGRCPSCGEWNTFAEEIISAPGRQ
                     ETLTPAAKAVPIGEISTAQQERISLNNTELERILGGGLVPGSVVLLGGEPGIGKSTLS
                     LQIPLHAPHLLTLYVSGEESARQIRMRAQRLLGGKDTEKMPDNCLVLCETMLENILEQ
                     AYAVKPSLLIVDSIQTAYSQHLESSPGSVTQVRECAALLLKYAKQTHVPVILVGHITK
                     EGSIAGPKVLEHIVDVVLQFEGDSTHAYRMLRGIKNRYGSTAELALFEMTGHGLRQVD
                     NPSEMFIPLHDEDLSGIAVAATIDGTRPFLIETQALVSTAAYGTPQRSANGFDTRRMN
                     MLLAVLEKRAGFRLAAKDVFLNMAGGLRVADPAVDLSVVAAILSSAFDLAIPARWCFA
                     AEVGLSGEIRPVPHLERRIAEANRLGFEKIFISSYNSRIHIPKGTQMEIEKVTGVGPL
                     ARMLFKI"
     gene            complement(2242..2880)
                     /locus_tag="PLM68_06490"
     CDS             complement(2242..2880)
                     /locus_tag="PLM68_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55044.1"
                     /translation="MKKLFFLIAVAACLLPLSAGKLAAQYHTYDYITYDKHEVFLQYG
                     APTFQELSTQIRKVTIVARDGNKYVPDRFVYTGIAALGYNYYTSPYMSLGGYVGISAA
                     MMEMAKTGTQTAVFKSSVLSITGLVTAHWIYYRTGMWELSAHAAAGITRWIDEQEMIV
                     SGSSDISTDTRKWRFAYHLSPLRVRWGGSFGVFADIGLGYKGIANAGVSVRF"
     gene            complement(2880..3884)
                     /locus_tag="PLM68_06495"
     CDS             complement(2880..3884)
                     /locus_tag="PLM68_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT55045.1"
                     /translation="MKWKKTLKVLLFAALALVLLYFSFRGIYWDDFIAGLRSADYFWI
                     LMSMLVGAFSFYVRAARWRIILLGLGKPVRRLDTFNGVGMAYLTNFALPRAGEVARCG
                     VVAQRAGLGFDKVLGTVVLERTFDVLCLVLITIAVAFLRMDVFGRFMVTQIWDPLMNR
                     LGGSSALAVILVAGPVLAVVLVLVLLRKPLGRIKVFRKTGDFFRGIWNGLVQSLKMPR
                     KGYFFFLTLLMWFLYICTSYCTILATPLRNQLNFTDALFLMVVGSFGWVVPVQGGIGA
                     FHFIVTLALVAVYGQTQTGGMVFATISHESQAVTMLFTGLFAVIYMSISKRNKPKEVQ
                     "
     gene            3954..4946
                     /locus_tag="PLM68_06500"
     CDS             3954..4946
                     /locus_tag="PLM68_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOT55046.1"
                     /translation="MKSILGIGNALTDILALLPDDSLLEHFHIPHGSMQHVDALTADR
                     IWQHLKPIGVQYVAGGSAANTITGTAVLGMPSGFIGKVGDDELGSLFYADQQRNGIKS
                     CLLKGIHSSGRAMVFVTGPDADRTFAVYLGAALELVPEDLRPEMFAGYDYFHIEGYLV
                     QNQHLVRRAVKLAREAGMVISIDMASYNVVESNNPFLHDIVENYVDIVFANETEALSF
                     TGKEPREAVREIAKICKVGVVKMGKLGSLVRSGDKEYEIDIWPGISIDGTGAGDLYAA
                     GFLFAHSQGLSLRKCGDIGSMVAAKVVEVIGPKIDIPRWKAVKQEIRQRIQDPE"
     gene            4948..6021
                     /locus_tag="PLM68_06505"
     CDS             4948..6021
                     /locus_tag="PLM68_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOT55047.1"
                     /translation="MSHPIIIAGPCSAETREQVWSAARQLAAFRLPPAAGSGGISFFR
                     SGIWKPRTRPGNFEGIGETGLPWLSDVQKELGLPVCTEVASARHVELVLKAGLQAVWL
                     GTRTTANPFLVEEIAKALKGAPLHVFVKNPINPDLGLWIGAVQRVLRYGPQKVYAIHR
                     GFSDYHIGQLRNAPLWRIPIDFKVQLPDIPLLCDPSHLSGDTSFIPDLAQRALDLNFD
                     GLMIEVHPSPAGALSDAVQQFTPAGFEKMMHSLSWRRSAPSEDLHSEEKIIIDAIRSR
                     IDVLDEDLVDLLAQRMSLVDQIGRVKKRGGIGIIQIERWNEVSSRTRKQAADKDLDDS
                     FIQEVYRAIHQQSIKRQEKIIRE"
     gene            6018..>6969
                     /locus_tag="PLM68_06510"
     CDS             6018..>6969
                     /locus_tag="PLM68_06510"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOT55048.1"
                     /translation="MSQLILSEDFSQLRALVPPGTKTLVLYDSQVTEWVRQAVDPSWE
                     LMALEGGETLKQWDRVEQTVSRMMEILADRSWFLIGMGGGTVCDFTGFLGSVYMRGMP
                     FGLVPTTLLAQVDAALGGKNGIHFGPYKNIIGCTQLPRWVFCNPQVLKTLPPEEFRCG
                     LAECIKHGAIASDSYFRFIEKEVAPCRDFRELPAGVLERLIRESQEIKMHVVEEDLYE
                     KGVRKALNFGHTFGHALAACYPQLSHGQAVAKGMVLAAACSADRGLLQPQQAQRLLQL
                     LVSCGLDPVLPCRERDIIPHMLHDKKKRGRDLDLVLLAGIG"
BASE COUNT         1638 a         1875 c         1968 g         1488 t
ORIGIN      
        1 gcagcagaca acaaggtgtc cccatccaca acgccattgg atagatgaag ccggcaccat
       61 tgcatcatat cggtcacggt ggagttgatg ccgccggctg ctcctatgac cgtaagccag
      121 tgcagcgcct gttcttcgcc ctccatgggg gctgtatgta tcttcccgtt ccggacggtg
      181 gtttcgtggg gggtgttccc gtttgcagta gccaggaatc catcttcatt ggtgctggta
      241 ttgtgcatac ccaggggggt gaagatcctt tcctgcaggt tgtcttccca gcttttgccc
      301 gtcacggcct cgatgatctt ctgggcaaca atgaacatca cattgttata gccatatgtt
      361 gtcctgacac tgtttgccgg gggaatgaac cttaacatgc ggtagacatc gtccctgccg
      421 tatcccaggc aggggatgta agtgccagcc tggccgggaa gcccggaacg gtgcaggaac
      481 aggtctctca cttccaggtt gtctgtcacc caggggtcgt acagggcaaa atcgggcagg
      541 taccatttga cggggtcatt ccagttgacc agcccgtcgt ccaccagcat ggccatgagt
      601 gtggccgtga atgatttgct gaccgaacct atctggaaca cggtgttgcc atctacgggc
      661 aacccgctgc cgttgcgttt ttctccaaat ccctggacaa aaacgatgga gtcatttttg
      721 atgaccgcca gggcagagcc ggtgatattc cattctttag ccacctgttg gaaatacggg
      781 gcgaaatcct gagcccccag gagtgcgggg aacaggagca gtgtgagtaa gaagtgtttt
      841 ttcatctgat aaagatataa atattttatc tttgtgtaat gagtaaagtc aagaaagcct
      901 ggttttgcca gaactgcgga gccgagtcgt caaaatggct cggacgctgc ccttcctgcg
      961 gggaatggaa tacttttgca gaagagatca tcagtgctcc gggcaggcag gaaaccctca
     1021 cccctgccgc caaagctgtg cccataggag agatttccac ggcacagcag gagcggatat
     1081 cccttaataa tacggagctg gagcgcatcc tgggcggggg gcttgttccc gggtctgttg
     1141 tcctgctggg aggggagccc gggattggga aatcaaccct cagcctgcag attccactgc
     1201 atgcacccca tctgctcacg ttatatgtgt caggggagga atcggcccgc cagatcagga
     1261 tgcgcgctca aaggctcctg ggcgggaagg acacggaaaa aatgccggac aattgcctgg
     1321 tgttgtgcga gaccatgctg gaaaacatcc tggaacaggc gtacgctgtc aagccttccc
     1381 tgttgatcgt ggattccata cagaccgctt attcgcaaca cctggaatcc tcgcccggaa
     1441 gcgtcaccca agtacgtgaa tgtgcggccc tgttgctcaa atatgcaaaa cagacgcatg
     1501 tcccggtcat cctggtaggg catatcacca aggaaggaag cattgccggt cccaaggtgc
     1561 tggagcatat cgtggatgtg gtgctgcaat ttgaagggga cagcacacac gcataccgca
     1621 tgctgcgagg catcaagaac cggtacggct ccacggcgga actggccctg tttgaaatga
     1681 ccggccatgg cctgaggcag gtggacaatc cgtccgagat gttcataccc ctgcacgatg
     1741 aagacctgag cggcattgcc gtggcagcta cgatagacgg gacacgaccc tttctgatag
     1801 aaacacaggc gctggtcagc acggctgctt acggaacgcc acagcgttcg gccaacggtt
     1861 ttgatacccg caggatgaac atgctcctgg cggtactgga gaaaagggcg ggattccgcc
     1921 tggcggccaa ggatgtgttc ctgaacatgg ccggcgggct gcgggtggcc gatccggcgg
     1981 tagacctgtc tgtggtcgca gccatccttt cctcggcctt tgacctggct ataccggccc
     2041 ggtggtgttt tgccgcagag gttgggctga gtggggaaat ccggcccgtc cctcacctgg
     2101 aacgcaggat tgccgaggca aaccgcctgg gatttgaaaa aatattcatt tcatcgtaca
     2161 attcccgcat acatatcccg aaagggacac agatggagat agagaaggtg accggggtgg
     2221 gaccgctggc ccggatgctc tttaaaatct gactgagacc cctgcgttgg caatgccctt
     2281 gtagcccagc ccgatatcgg caaatacccc gaaagagcca ccccatctca cccgtagcgg
     2341 gctcagatgg taggcgaacc gccacttgcg ggtatcggta gaaatgtctg aagaacccga
     2401 tacaatcatt tcctgttcat ctatccagcg ggtaataccg gcggccgcgt gtgccgaaag
     2461 ctcccacatg ccggtccggt aatagatcca gtgagccgtt accagcccgg tgatgctcaa
     2521 cacactgctt ttgaaaaccg ctgtttgggt gccggtcttc gccatttcca tcatggcggc
     2581 actgatgccc acgtaccctc ccaggctcat gtaaggggaa gtatagtaat tgtagcctaa
     2641 agcggcaatc cccgtataga caaaacggtc tggaacgtac ttgttcccgt ccctggccac
     2701 tatcgttacc ttgcggatct gtgtactcaa ttcctggaac gtgggagccc cgtattgcag
     2761 gaaaacttcg tgcttgtcat acgttatgta atcgtacgta tggtattgtg ctgccagctt
     2821 tcccgcggac aacggcagca ggcaggcagc gacagcgatc agaaaaaaca gttttttcat
     2881 tattgtacct ctttgggttt atttcttttg gatatgctca tatagatcac ggcgaacaat
     2941 ccggtaaaca gcattgtaac agcctgtgat tcatggctaa tggtggcaaa gaccatcccg
     3001 cctgtctggg tctggccgta tacggccacc agcgccaggg tcactatgaa atgaaaggct
     3061 cctatcccgc cctgaaccgg gacaacccat ccaaaactcc ccacgaccat caggaacagc
     3121 gcgtctgtga aattcagctg gttcctcagg ggagtagcca ggatggtgca gtaactggta
     3181 catatgtaaa ggaaccacat aaggagggtg agaaagaaaa aatatccctt gcggggcatt
     3241 ttcaggcttt gcaccagtcc attccagatg ccccggaaaa aatctcccgt ctttctgaaa
     3301 accttgatcc ggcccagtgg tttgcggagc aggaccagga ccagcacaac cgccaggacg
     3361 gggcctgcaa ccaggatgac ggccagtgca gaagagcctc caagccggtt catgagaggg
     3421 tcccagatct gggtgaccat gaaacggccg aaaacatcca tgcgcaggaa ggcaacggct
     3481 atggttatca gcaccaggca tagtacatca aaagtccttt ccaggactac ggttcccagg
     3541 actttgtcaa agcccagacc ggcacgttgt gcgactaccc cgcatcgggc aacctctccg
     3601 gccctgggca gggcgaaatt tgtcagatac gccataccca cgccgttgaa ggtatccagc
     3661 cttcttacag gttttcccaa accaagcagg atgatcctcc agcgggcagc ccggacataa
     3721 aaggaaaagg ctcccaccag catggacatc aggatccaga aataatccgc agagcgcagg
     3781 cctgctatga aatcatccca atagatgccc ctgaaggaaa agtaaagcaa caccagggca
     3841 agagccgcaa acagcagtac tttcagggtt tttttccact tcatgacccg gcaaaggtac
     3901 aaaaaaagga cattcggaaa attagattaa atttgctgta aatagtggtt tttatgaaat
     3961 ctatccttgg aatcgggaat gccctgacag atatccttgc tttacttccc gacgactccc
     4021 tgctggagca tttccacatt cctcacggca gcatgcaaca cgtagacgct ctcaccgctg
     4081 acaggatctg gcagcacctc aaacccatag gggtacagta tgtggcgggc gggtctgcag
     4141 caaacaccat tacgggaacc gccgttctgg gcatgccctc gggcttcata gggaaggtgg
     4201 gagacgacga gctgggctcg cttttctatg cagaccaaca gcgcaacggc atcaaatcct
     4261 gcctgctcaa agggatccac tcctcgggac gggccatggt ttttgtaact ggacccgatg
     4321 cggaccgcac ctttgccgta tacctggggg ctgccctgga gctggttccg gaagacctca
     4381 ggccagaaat gtttgcagga tacgattatt tccacataga gggatacctg gtccagaatc
     4441 agcacctggt gcgcagggca gtcaagctgg cacgcgaggc gggcatggtc atttccattg
     4501 acatggccag ctacaatgtg gtggaaagca acaacccctt cctgcacgac attgtggaga
     4561 attacgtgga cattgtcttt gccaatgaga cggaagcctt gtcctttacc ggcaaggaac
     4621 cccgggaagc cgtgcgtgag atcgcgaaga tctgcaaggt gggggtggtc aagatgggaa
     4681 agctgggttc acttgtcaga agcggggaca aggaatatga aattgacata tggcccggga
     4741 tttccataga cggaaccggt gcaggggact tgtatgccgc aggtttcctt ttcgcccatt
     4801 cccaagggct ttccctgcgc aaatgtggtg acatagggtc catggtcgcc gccaaagtgg
     4861 tagaggtcat tggtcccaaa atagatattc cccgctggaa agcggtaaaa caggaaattc
     4921 gtcaacgtat acaggaccct gaataacatg agccatccta tcatcatagc aggcccctgc
     4981 agtgccgaaa cacgggaaca agtatggtca gcagccagac agctggcagc attcaggctt
     5041 ccccccgcgg ccggaagcgg gggcatatcc tttttccgtt ccggaatatg gaaacccaga
     5101 acgcgtcccg gcaattttga aggaatcggg gaaacgggac ttccctggct gagcgatgtc
     5161 cagaaggaac ttggattacc ggtctgtacc gaagtggcct ctgcccgtca tgttgagctg
     5221 gtgctcaagg caggacttca agctgtctgg ctgggtaccc gcaccacagc caatcctttc
     5281 ctggttgagg aaatagccaa ggccttgaaa ggcgcacccc ttcatgtctt tgtaaagaat
     5341 cccatcaatc ccgacctggg gttgtggata ggggccgttc agcgtgtcct caggtatggc
     5401 ccgcaaaagg tgtatgccat tcaccgcggt ttcagcgatt accatatcgg acaactgcgc
     5461 aacgctcccc tgtggcgcat tcccattgac ttcaaggtgc agctgcccga catccccctg
     5521 ttgtgcgatc cctcgcacct gtccggagac acctccttta tccccgacct ggcacaaagg
     5581 gctctggacc tgaattttga cggattgatg atagaggtgc atccctcccc cgcaggggcg
     5641 cttagtgatg ccgtacagca attcacgccg gccgggttcg aaaagatgat gcattccctt
     5701 tcctggagac ggtctgcgcc ttctgaagac ttgcactcgg aagaaaaaat aatcattgat
     5761 gccatccgtt cccggataga cgtgctggac gaggacctgg tggacctgct ggcacagcgg
     5821 atgtcgcttg tggaccagat tggccgtgtg aagaagcggg gcgggatagg gattatacag
     5881 atagagcggt ggaacgaagt gtcttccagg acccggaagc aggccgcaga taaagatctg
     5941 gacgacagct tcatccagga agtgtaccgg gccattcacc agcaatccat caaacgccag
     6001 gaaaagataa tccgcgaatg agccagctga ttttatcgga agatttttca caattgcgcg
     6061 cgcttgttcc cccgggaaca aaaaccctgg ttctttatga cagccaggtg acagaatggg
     6121 tgcggcaggc cgtggacccg tcctgggagc tgatggccct ggaagggggc gagacgctca
     6181 agcaatggga ccgggtggag cagacggtgt cccggatgat ggagatcctg gcagaccgtt
     6241 cctggttcct gataggcatg ggcggaggta cggtgtgcga tttcacaggt ttccttggtt
     6301 ctgtatacat gcgcggaatg ccttttggct tggttcccac cacgttgctt gctcaggtgg
     6361 atgccgccct gggaggcaag aacgggatac atttcggccc gtacaaaaac atcatcgggt
     6421 gcacgcagct gccccggtgg gttttctgca atccacaagt tctgaaaaca cttcccccgg
     6481 aggaattccg ctgcgggctg gcagaatgta taaagcacgg ggccatcgcc tcggactcct
     6541 atttcagatt tattgagaag gaagtggcac cgtgcagaga tttcagggaa ctgccggccg
     6601 gggtgctgga gcggctaatc cgtgaaagcc aggaaatcaa gatgcacgtg gtggaagagg
     6661 acctttacga aaaaggagta aggaaggccc tcaatttcgg acacacgttc ggacatgcac
     6721 tggccgcctg ttatccacag ctctctcacg gtcaggctgt tgcaaaaggc atggtcctgg
     6781 cagccgcgtg ttcggcggac aggggccttc tgcagccgca acaggcacag cgcctgctgc
     6841 agctgctggt ttcctgcggc ctggatcctg tgctcccctg ccgcgaaagg gacatcatcc
     6901 cacacatgct gcacgacaag aaaaaacgcg gcagggacct ggaccttgtt ttgcttgcgg
     6961 ggattggcc
//
LOCUS       DAOSOF010000104         6965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_10892_length_6965_cov_5.009117, whole genome shotgun sequence.
ACCESSION   DAOSOF010000104 DAOSOF010000000
VERSION     DAOSOF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10892_length_6965_cov_5.009117"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /gene="galE"
                     /locus_tag="PLM68_06515"
     CDS             complement(<1..831)
                     /gene="galE"
                     /locus_tag="PLM68_06515"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT55049.1"
                     /translation="MKNILITGGAGYIGSHTAVELIHAGYGVVIADNLSNADTTCLEG
                     VKKITGRDIPFYKADCCRKDQLLPIFREHRIDGVIHFAAFKAVGESVEKPLMYYHNNL
                     LSLVTLLEVMVENGGGHVVFSSSATVYGQPPVLPVTEDTPRMPATSPYGNTKQICEDI
                     LADAVKAYASVKVVILRYFNPIGAHPSALIGELPRGIPNNLVPFITQTAAGVRKELHI
                     FGSNYNTPDGTALRDYIDVVDLAAAHVSALKRMEAYDKPGLCEVFNVGTGKPLSDMEL
                     V"
     gene            complement(918..>2285)
                     /locus_tag="PLM68_06520"
     CDS             complement(918..>2285)
                     /locus_tag="PLM68_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOT55050.1"
                     /translation="KVILVREETNPEDVEGMRVADGLLTARGGMTSHAALVARGWGKC
                     AIVGCEAVHIDLAKRVLTMNGKKYPEGSVFSLNGTRGFVYDKAIDTMDASENPRFQEF
                     MKMVDSFRTMGVRTNADTPEDARMALNFGAEGIGLFRIEHMFYGSNSEAPLSKLRKMI
                     LSENVGERKLALHELAPYVRAAAKETLRVMDGLPLTFRLLDPPLHEFVPHTLDKQKEL
                     AKELGISVEDVLKRGEMLHEENPMMGHRGVRLGITYPEISEMQFRAIFTATAELIKEG
                     LRPKPEIMVPVTVSVKELDDQKIIADRVHKEVQDQFGLTFEYLYGTMIEIPRATIVAD
                     EMAKTAQFFSFGTNDLTQMTFGFSRDDIGSFMGDYIQKKILPFDPFLHIDEPSVGRLI
                     EEGITKGRSTTPNLKIGICGEHGGDPQSIQFCYKMGMTYVSCSPFRVPIARLAAAQAA
                     IWAAM"
     assembly_gap    2286..2385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2386..3790)
                     /locus_tag="PLM68_06525"
     CDS             complement(<2386..3790)
                     /locus_tag="PLM68_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT55051.1"
                     /translation="MSEIKRVYSFGGKTADGDGSMRNLLGGKGANLAEMCLLGIPVPA
                     GFTITTECCTEYYALGCNYPKGLAGEVAQALMKAEDVMGARFGDPENPLLVSCRSGAR
                     SSMPGMMETVLNIGLCSATIPGLIKKTNNPRFVYDAYRRLIMMFSDVVMEKAEGIEPA
                     EGQGIRVQLDALLHEMKVEKGYKSDTDLTEEDLITLCDMFKKRVVKVLGKPFPDDAME
                     QLWGGIGAVFKSWNGKRAISYRRIEGIPDEWGTAVTVQSMVFGNMGDNSATGVAFSRN
                     PATGEAKFYGEWLINAQGEDVVAGIRTPNPLNESTKNDQNRHLASLETTMPKVYGELN
                     QIQKNLEAHFRDMQDIEFTIQDGRLWMLQCRIGKRTGVAALNMAMDMLLEGLIDRKTA
                     VLRVAPNQLDELLHPILNPNSEKAAQVVGKGLPAGPGGAVGQIVFTAEDAVTLSKKGK
                     KVILVREETNPEDVEGMR"
     gene            complement(3856..5187)
                     /locus_tag="PLM68_06530"
     CDS             complement(3856..5187)
                     /locus_tag="PLM68_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT55052.1"
                     /translation="MSNNPKIFVLDTNVLLHDHKALGNFQENDVVIPIIVLEELDKFK
                     KGNDQINFNAREFARSLDKILGDKMLNGWISMGPGRGRLKVELGHPFPDMMQDSFYED
                     TPDHRILVVAQYMKENYPERMVILITKDVNLRIKSKALGIEAQDYLTDKVRDESIVSI
                     SKGVIKLNRVTPQTIAKLYQTPEGLPGKDIRLKGAHHNQYYILRNKDGSVMARYQAST
                     KCLIPVPGHRAYGISPRNSEQNFALDALLDPAISLVSLTGIAGTGKTLLALAAALEQE
                     SDYDQIMLSRPVIPLQNQEMGYLPGDIKEKMAPYMLPLLDNLSVIKGSLKANSREFVR
                     IEEMVKNEKLLISPLAYIRGRSLSKVFFIVDESQNLTPHEVKTIITRAGEGSKLVFTG
                     DIYQIDQPYLDMHSNGLTYLTDKMLGQELFAHVNLVRGERSRLADLAGNIL"
     gene            5243..6520
                     /locus_tag="PLM68_06535"
     CDS             5243..6520
                     /locus_tag="PLM68_06535"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT55053.1"
                     /translation="MEFSEALSFLIGNFPQYQKIGIEGYKPYPQRMLSLAAWLGNPQD
                     RYPCIHIAGTNGKGSVSHMLAAIMQAAGLSTGLYTSPHLTDFRERIKVNGKMISQAEV
                     IDFLERFSRERMGELGEELPSFFDMTTAMAFHHFALQKTDIAIVETGLGGRLDSTNII
                     TPPSVLLSVITNIGLDHCDLLGDTIAKIASEKAGIIKPGVPVVVGEYDPEALPVIAAR
                     AVRLKAPLFRAFEGSYPSYDIEGDTLYQQKNLKTALCAVDVLKETNPGLSSRLTPQAI
                     ATGLEHFESVTGLRGRWERLSLFPAVIADTSHNAHGMKYLKEQLAKERYQRLYIVLGV
                     VRGKDLESITSLLPQDAYYYFTQAHIPRALPFTELAAWCRRAGLKGEEVPTVEEAYQK
                     ACAAASAQDLILVGGSTFTVADLLENFFCKGKN"
     gene            6565..6639
                     /locus_tag="PLM68_06540"
     tRNA            6565..6639
                     /locus_tag="PLM68_06540"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6600..6602,aa:Val,seq:tac)
BASE COUNT         1620 a         1719 c         1699 g         1827 t
ORIGIN      
        1 gacaagttcc atgtcagaga gcggtttacc agtccccaca ttgaacacct cgcacaatcc
       61 cggtttgtca taggcctcca tgcgtttcag ggccgagaca tgggcggcag caagatccac
      121 cacatcaata taatccctga gagccgtccc gtccggggta ttgtaattgc ttccgaatat
      181 gtgcaattcc ttgcgcacgc cagcagcggt ctgcgttatg aaaggtacca ggttgttggg
      241 aatgcccctg ggaagttcac cgatcagggc ggaaggatgt gccccgatgg gattaaaata
      301 cctgaggatg accactttca cggatgcata agcttttact gcatcggcca ggatatcctc
      361 acatatttgt ttcgtgttac cgtaaggact tgtggccggc attctgggag tgtcttcagt
      421 aaccggcagc accgggggct gtccgtaaac ggtggcggat gatgagaata ccacatgacc
      481 tccgccattt tccaccatca cttccagtaa ggtgaccagg gacaacaggt tgttatggta
      541 atacatgagc ggtttttcca cactttctcc caccgccttg aaagctgcaa agtgaatgac
      601 accgtcaatc ctgtgttctc ggaatattgg caggagctgg tcttttctgc agcaatcggc
      661 cttatagaaa ggaatatctc tgccggtgat ttttttcaca ccctccagac aggtggtatc
      721 tgcattggaa aggttatctg caatgaccac tccgtatcct gcgtggatga gttccacggc
      781 agtatggctt cctatatagc cggcgccccc ggtgatgagt atgtttttca taatgacaaa
      841 gatacgaaaa agaagaaaaa ggctcctatg ggagcctttt tcttcttttt ggaaacaatc
      901 acctgattgt ttcagactta catggctgcc cagatggcgg cctgtgctgc agccaggcgg
      961 gcgataggca ccctgaaggg ggaacaggaa acataagtca tacccatctt gtaacagaac
     1021 tggatggact gggggtcccc tccgtgttct ccgcatatcc cgatcttgag attcggagtg
     1081 gtggaacggc ctttggtgat tccctcttct atgagcctgc ctacagaagg ctcgtcaata
     1141 tgcaggaacg ggtcaaaggg gaggatcttc ttctggatgt aatcacccat gaatgacccg
     1201 atatcgtccc ttgagaaacc gaaggtcatc tgtgtcaggt cgtttgtccc gaacgagaag
     1261 aactgagccg tcttggccat ctcatctgcc actattgttg cccggggtat ctctatcatg
     1321 gtgccataga ggtactcgaa ggttagtccg aattgatcct ggacttcttt gtgcaccctg
     1381 tcggcgataa ttttctggtc atccagctct ttcacactga ctgtaacggg aaccatgatc
     1441 tccggtttgg ggcggagccc ttccttgatc aactcggcag tggctgtgaa aatagcacgg
     1501 aactgcattt cagaaatctc gggatatgtt attccaagac gcacaccacg gtgtcccatc
     1561 ataggatttt cctcgtgcag catttctccc cgtttgagta catcttccac gcttatgccg
     1621 agttctttgg ccagttcttt ctgtttgtca agcgtatggg gaacaaattc gtgcaacgga
     1681 ggatcaagca ggcggaaggt aagcggcagt ccgtccatga cccggagcgt ttccttggca
     1741 gcagctctga catagggagc cagttcatgc agggccagtt ttctctcacc cacattttcc
     1801 gaaaggatca ttttgcggag cttgctcaag ggggcttctg aattcgagcc gtagaacatg
     1861 tgctcgatac ggaaaaggcc tattccctcg gccccgaaat tcaaggccat acgggcatct
     1921 tccggtgtgt ctgcgttggt gcggactccc atggtacgga aggaatccac cattttcatg
     1981 aattcctgga accggggatt ttcggaggca tccatagtat cgatcgcctt gtcatataca
     2041 aagccacgtg ttccattaag actgaaaaca gagccttcgg ggtatttttt cccgttcatg
     2101 gtcagaacgc gttttgcaag atcaatgtgc acggcttcac agcctactat ggcgcatttt
     2161 ccccatccgc gggctaccag ggcagcgtgg gatgtcatgc ctccgcgagc ggtcagcaga
     2221 ccgtcggcaa cacgcattcc ttccacgtct tcgggatttg tctcttccct gaccaatatg
     2281 actttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncacgc attccttcca
     2401 cgtcttcggg atttgtctct tccctgacca atatgacttt cttgcctttc ttgctcaatg
     2461 taacggcgtc ttcagccgtg aaaacaatct ggcccacggc gccgccggga ccggccggca
     2521 gacccttgcc caccacttgt gcggcctttt cagaattggg attcaatatg gggtgaagca
     2581 gttcgtccag ttgattgggc gcaacgcgca gcacagctgt cttgcggtcg atcagacctt
     2641 ccagcagcat atccatggcc atattcaggg cggcaacccc ggtgcgtttg cctatgcggc
     2701 attgcagcat ccacaggcgt ccgtcctgga tggtgaactc gatgtcctgc atgtcacgga
     2761 aatgagcttc caggttcttc tggatctggt tcagttcgcc atatactttg ggcatcgtgg
     2821 tttcgagtga tgcgagatgc ctgttctggt catttttggt ggactcgttc agggggttgg
     2881 gtgtgcggat accggcaacc acatcttctc cctgtgcgtt gataagccat tcaccgtaga
     2941 acttggcttc tccggtagcc gggttacgtg aaaaggcaac accagttgcc gaattgtcac
     3001 ccatatttcc aaataccatg gattgcaccg tcacagcagt accccattca tcgggaatgc
     3061 cttctatacg gcgataagag atagcgcgtt tcccgttcca tgatttgaac acggcaccta
     3121 tgccacccca gagctgttcc atggcatcat cggggaatgg cttgccgagt acttttacca
     3181 cccgtttttt aaacatgtcg cagagtgtga tcaggtcttc ctctgtaagg tcggtatcgc
     3241 ttttatatcc tttttccact ttcatctcat gaagcaacgc atccagttgc actcgtatgc
     3301 cttgtccttc tgcgggttca atcccttctg ccttttccat gaccacatct gagaacatca
     3361 taatcaggcg gcggtatgca tcgtacacga acctgggatt gtttgtcttt ttaatcagcc
     3421 cgggaatggt agcagagcac aggccaatgt tcaacacggt ttccatcatg ccgggcatgg
     3481 aactcctggc gcctgaacgg caagaaacga ggagtggatt ttcagggtca ccaaaccggg
     3541 ctcccataac atcttcagct ttcatcagag cctgtgcgac ttctcctgcc aagcccttgg
     3601 ggtagttgca gcccagggca taatattcgg tacaacattc tgttgttata gtgaaacctg
     3661 caggaacagg tattcccaag agacacattt ctgccaggtt ggcacctttg ccacccagca
     3721 gatttcgcat ggatccgtca ccatcggcag tttttccgcc aaagctgtaa actcgtttga
     3781 tttctgacat aaaaattata taaggttaat aaattgaata ttatgggatt tggggctgca
     3841 aaaataataa aaaagttaca atatgttccc cgccagatcg gccaatcggc tccgttcccc
     3901 cctgacaagg tttacatgcg caaagagttc ctgtcccaac attttgtctg tcaaataagt
     3961 aagaccgttt gaatgcatgt caagataggg ttggtcaatt tggtagatgt cgccggtgaa
     4021 aaccagcttg ctcccttctc cagccctggt aattatggtt ttaacttcat gtggtgtaag
     4081 gttctgtgat tcatcaacta tgaagaaaac tttcgacaaa cttcttcccc ggatgtatgc
     4141 cagcggtgat atcagcaatt tttcattctt taccatttct tctatgcgga caaattcacg
     4201 gctgttggct ttcaggctgc ctttgataac acttagatta tccagcaggg gcaacatgta
     4261 gggagccatt ttttccttga tgtccccggg cagatagccc atttcctggt tttgcagggg
     4321 aataacaggc cgggatagca tgatctggtc atagtcgctt tcctgttcaa gggcagccgc
     4381 caaggcaagc aaggtcttcc cggtaccggc aatcccggtg agggaaacaa ggcttatggc
     4441 gggatcaagc agggcatcca gggcaaaatt ctgttccgag ttacgggggg agatgccata
     4501 ggccctgtgg cccggaacgg gaatcaggca ttttgtggat gcctggtatc gtgccatgac
     4561 cgatccgtcc ttgttcctga gtatgtaata ctggttgtgg tgtgctcctt taagacgtat
     4621 gtcctttcca ggaaggcctt ccggtgtctg gtacaatttg gcaatggtct ggggtgtaac
     4681 cctgttcaat ttgataacac ctttggagat gctgactatg ctttcgtcac ggaccttgtc
     4741 tgtcaggtaa tcctgtgctt ctattcccag cgccttagac ttgatacgca ggttgacatc
     4801 ttttgtgatc aggatcacca tgcgctcggg atagttttct ttcatgtatt gtgccacaac
     4861 aaggatacgg tggtcggggg tatcttcata aaaagaatcc tgcatcatgt caggaaaagg
     4921 atgacccagt tccaccttca ggcgtcccct gccgggaccc atactgatcc acccattcaa
     4981 catcttatcc cccaggatct tatccagaga acgcgcaaat tcacgggcat tgaagttgat
     5041 ctggtcattg ccttttttga acttatccag ttcttcgagt acgataatgg gtatgaccac
     5101 atcattttcc tggaaattac cgagtgcctt gtggtcgtga aggagcacat tggtatcgag
     5161 tacgaatatt tttggattgt ttgacattag ttgatattta accaacaaag ataggaaaaa
     5221 ttatgtctaa attcgcagcg tcatggaatt cagcgaagcc ttatcttttc tgatcgggaa
     5281 ctttccccag taccaaaaaa taggtataga aggatacaaa ccgtatccgc aacgcatgct
     5341 ctccctggct gcctggcttg gaaatcccca ggacaggtat ccctgtatcc acattgcggg
     5401 aactaacgga aaaggctctg tttcccatat gcttgcagct atcatgcagg cagcaggact
     5461 tagtacgggg ctgtacacat caccccatct gacagatttc agggaacgga taaaagtaaa
     5521 cgggaaaatg atttcccagg ctgaggtaat tgattttctg gaacgttttt cacgtgaaag
     5581 gatgggggaa ctcggggaag aactgccctc tttttttgat atgaccacag ccatggcttt
     5641 tcatcacttt gccttgcaaa agacagatat tgccattgtc gagactggcc ttgggggacg
     5701 tctggattcc accaacatca tcactcctcc atcagttctg ctttctgtca ttaccaatat
     5761 tgggctggat cattgcgact tgctgggaga taccatagca aagatagcga gtgaaaaagc
     5821 agggatcatc aagccgggag ttccggtagt ggtgggcgag tatgaccccg aggcattgcc
     5881 tgttattgcc gcccgtgctg tccggttgaa agctcccctt ttccgggctt ttgaaggatc
     5941 ttacccttcc tatgacattg aaggagacac cttgtatcag cagaaaaatc tgaaaaccgc
     6001 actgtgtgca gtggatgtgc taaaagaaac caatcctggc ttgtcatccc ggctgacacc
     6061 ccaggcgatt gctacaggac tggaacattt tgaatcagtt acaggcctgc gcggaaggtg
     6121 ggaacgtctt tccctcttcc cggccgttat tgccgacacg agccataatg cacacgggat
     6181 gaaatacctg aaagaacaac tcgcaaaaga aagataccaa cggttgtata ttgtccttgg
     6241 tgtggtaagg ggaaaagacc tggaatccat cacatcgctt ttaccacagg atgcctatta
     6301 ttattttaca caagcacaca tccccagagc gctgcctttt accgagctgg ctgcttggtg
     6361 caggagagca ggcttgaagg gagaagaagt gccgacggtc gaagaggcct atcaaaaggc
     6421 atgtgcagca gcctccgccc aggatttgat cctggtggga ggcagtactt ttaccgtggc
     6481 ggatctgctg gaaaactttt tttgcaaggg aaaaaattaa gcatatcttt gcagtccatt
     6541 tagcatacct ttgctaatgc acatgggagc ttagctcagc tggtttagag cacctgcctt
     6601 acaagcaggg ggtcatgagt tcgaatctct tagctcccac aacaaaacgg ccggccaaag
     6661 atcaaatttg gccggctgct tttttgccta ggctctcaca cttatactgt cttctgctgt
     6721 cgttgctacc ataaagaacc gggagctttt attacgccga ggctcccggt tctttatggt
     6781 agcaacgaca gctagcagca agttgttgaa gcaccggcga gaaagaaaaa cttaacgttc
     6841 ttgaaatgca aggatcacag agtacgaggt atcctacctg agtatgcagt cttcccaacc
     6901 catggcatca accatgcgca tccagtgcag ggcttcccta cgggtgcgga agtattggga
     6961 tacat
//
LOCUS       DAOSOF010000105         6808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_11240_length_6808_cov_5.040871, whole genome shotgun sequence.
ACCESSION   DAOSOF010000105 DAOSOF010000000
VERSION     DAOSOF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11240_length_6808_cov_5.040871"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..928
                     /locus_tag="PLM68_06545"
     CDS             32..928
                     /locus_tag="PLM68_06545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT55054.1"
                     /translation="MQLLCPVSWPDYRLVDCGNQEKLEQFGPYLIIRPEPKALWASSL
                     PAGEWMSRAHLRFVQGAGFGKAGKEDSGTWEKLKQVPDRWVIGYKKLFRLRMGLTAFK
                     HVGVFPEQAPNWDYIYQQVQKIREKVPQPAPKVLNLFAYTGAASLAARAAGAEVVHLD
                     AVRQVITWGRENMELSGMDGIRWILEDAFRFVKREVRRGNVYQGLILDPPAYGHGPEG
                     ERWKLDDCLLSLLQECSRILAGDNAFVVLNLYSNGYSALLADTLARSCFPASGNYTFG
                     ELYLKDTFGKRLPLSVFSRIEL"
     gene            950..1837
                     /gene="lgt"
                     /locus_tag="PLM68_06550"
     CDS             950..1837
                     /gene="lgt"
                     /locus_tag="PLM68_06550"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT55055.1"
                     /translation="MNLLAVYWDVDPVIFSIWKIDVRYYSLFFIIAFAIGYYIFKGFF
                     KREGVPLKLLDPLLYTLILGTLVGARLGHCFFYQPDYYLANPIEILYVWEGGLASHGG
                     AIALLLAMWWFARHYGRKYQFNFLWIMDRLGIATALAGFFIRMGNLMNSEIYGNETTL
                     PWGFIFLRNGEVVPKHPTQLYEALSYLLLFVLLLYLYRKKLPRLKEGTLFGLFLLLLF
                     AARFFIEYIKEPQVAFEETMALNMGQLLSIPFIFAGAILLLYSIMRGRPAMRVEPAGK
                     QKVKKQEKKPPLSSTRGTY"
     gene            1854..2345
                     /gene="smpB"
                     /locus_tag="PLM68_06555"
     CDS             1854..2345
                     /gene="smpB"
                     /locus_tag="PLM68_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT55056.1"
                     /translation="MPKAKSGVVIKNKRASFEYEFIDTYTAGIVLTGTEIKSIRAGKA
                     SLVDSYCYFEGKELFVKNMNIADYWWGSYNRHEPRRDRKLLLTRHELNKLYRATREKG
                     LTIVVTKLFIADNGYAKLNIALARGKREYDKRESIREKDQKRDSDRALLNRSGIRDKK
                     GEG"
     gene            complement(2506..5346)
                     /gene="gcvP"
                     /locus_tag="PLM68_06560"
     CDS             complement(2506..5346)
                     /gene="gcvP"
                     /locus_tag="PLM68_06560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOT55057.1"
                     /translation="MALSFTERHNGPRPEQQKEMLSVIGVDSLDELIDKTVPAQIRMK
                     DPLPLEEGMSEYEFLMHLKAMASKNLPFRSLIGMGYYGTASPPVILRNIFENPGWYTS
                     YTPYQAEISQGRLEALFNFQTMVCSLTGFNLANSSLLDEATAAAEAMRMMLELRSRTL
                     VKEGRNVILVDRNIFPQTLAVMETRALGLGIEIVTEDLTGELLPVFEKHGPRLFGVMV
                     QYPGADGQLCDHSALCRLAHERELLVTAVCDLLSLAVLQEPAAWSADIAAGTAQRFGL
                     PMAFGGPTTGYLACPDKYRRNIPGRIVGLSVDRLGNPAYRLALQTREQHIKRERATSN
                     ICTATALMATMAGMYAVYHGSKGIRRIAQNGRHYAHTAAAILSKYGFSVKHKHFFDTI
                     ELTGVDTARIKANAEALKINFFYPDPQSVRISFDELTNERELEKILEIFNIPLEEKTA
                     LPEIPSFMERTAPALEEPVFNTYHAETEMMRYIKTLETRDISLAHSMIPLGSCTMKLN
                     AAAEMMPLSQMEWTAVHPFAPSWQAEGYLQLIREVEQQLSIITGLDACSLQPNSGAAG
                     EYAGLMVIRAFQRARGEGHRNIALIPSSAHGTNPASAAMAGLEIVIVGCDDQGNIDVE
                     ALRQKALEHRDNLSCMMITYPSTHGVYEAGICEISRIVHENGGLVYIDGANMNAQVGI
                     TNPGMLGADVCHLNLHKTFAMPHGGGGPGVGPICCTRELARYLPGHPLVNSGGGSPVA
                     ASPYGSPLLIPITYGYIKMLGREGLRRASEVAILNANYLSRILAPHYATLYTGRNGYV
                     AHECIIDLRHFSKTYGVDATDVAKRLMDFGFHAPTLSFPVHETLMIEPTESESKEELD
                     RFAQAMLTILDECKCLASGDDSCGTAEDNLLKNAPHTAVEVCSDNWTHPYARQKAAYP
                     LPWIVMHKFWPLVTRVDNGYGDRNLVCCSVIR"
     gene            complement(5368..>6808)
                     /gene="rodA"
                     /locus_tag="PLM68_06565"
     CDS             complement(5368..>6808)
                     /gene="rodA"
                     /locus_tag="PLM68_06565"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT55058.1"
                     /translation="TSPAMMRRNDKLIRKLDWWTVATYMVLVLIGWISVFAAVYDPEH
                     AHIFDTSQRYGLQMIWILGGWFLAVVVLLVPARVYALISNLIYIIGLLLLLAVLVFGV
                     ERGGSNSWLVFGPVHFQPAEMTKVFVAIGLAGVMSRPHFHIRSWRGLLQVTAMIVIPA
                     ALILLEKETGLALVLSAFIIVLYREGLPGWIPVTGIVAIILFLLSIVWDPLFVIWLCL
                     GVCFIISLLLSSRKMPVLIIFGSFAAVWFLVPLVMRWFSADLFSYMDACQWLLALAAV
                     LVVVLFIIAMRKKIAYLKILLLCFVSSIILIFSVGYIFNNILKPHQQARIETLLGIDK
                     DIYGTGYNVHQSKVAIGSGGFAGKGFLHGTQTKYNFVPEQSTDFIFCTIGEEWGFLGA
                     SLVIFLYTFLLVRIIRIAERQRQPFCRIYGYGVASCIFFHVFINIGMTIGITPVIGIT
                     LPFISYGGSSLWTFTLLLFILLKMDASND"
BASE COUNT         1621 a         1742 c         1874 g         1571 t
ORIGIN      
        1 tgcaaagata caatttttat atctttaccc tatgcaacta ttatgtcctg tgtcatggcc
       61 cgattaccgg ctggtggatt gcgggaacca ggagaagctg gagcagttcg gcccttatct
      121 gatcatacgg cccgagccga aagccttatg ggcaagttca ctcccggccg gggaatggat
      181 gagccgggcc caccttcgtt ttgtgcaggg tgccggtttc gggaaagccg gcaaggagga
      241 cagcggaacc tgggaaaaac tgaaacaggt gcctgaccgg tgggtcatag ggtacaagaa
      301 gcttttcagg cttcgcatgg gattgaccgc attcaagcac gtcggggttt ttccggagca
      361 ggcacccaat tgggattata tataccaaca ggtgcagaag atccgggaaa aggttccgca
      421 acctgctccc aaagtgctca acctgtttgc ttatacagga gccgcttcgc tggccgcgcg
      481 tgcagccggg gccgaggtgg tgcacctgga tgcggtcagg caggtgatca cctgggggcg
      541 ggaaaacatg gaactgagcg gtatggacgg tatccggtgg atactggaag atgccttccg
      601 gtttgtgaaa agggaagtac gcaggggaaa cgtctaccag ggcctgatac tggacccgcc
      661 cgcctatggg catgggccgg aaggggaaag atggaaactg gatgactgcc tgttgtccct
      721 gttgcaggaa tgttcccgta ttctggccgg ggataacgca tttgtggtgc tgaatttgta
      781 ttccaacggc tattcggcac ttttagccga cacccttgcc agatcttgtt ttcccgccag
      841 cgggaactat accttcgggg aattgtacct gaaagacact tttggcaagc gcctgcccct
      901 gagtgtgttt tcaaggattg aattataaac agatttaaaa aatacagcta tgaacctgtt
      961 ggccgtatac tgggatgtgg accccgtcat attttccatc tggaagatag atgtccgcta
     1021 ttacagtcta ttcttcatta ttgcctttgc cattgggtat tacattttca agggattctt
     1081 caaaagggag ggcgttccac tcaaattgct ggatcccctg ctctataccc tcatcttagg
     1141 gacgctggta ggggcgcgcc tggggcattg ctttttttac cagccggact attacctggc
     1201 caaccccata gaaatcctct atgtttggga aggaggcctt gcttcgcacg gcggtgccat
     1261 tgccctgctc ctggccatgt ggtggtttgc ccgtcattat ggcagaaaat atcagttcaa
     1321 ttttctctgg atcatggacc gtcttgggat agccaccgcc ctggcaggct ttttcatccg
     1381 tatgggaaac cttatgaatt ccgagatata cgggaacgaa accacccttc cctggggttt
     1441 tatctttcta cgtaatggag aagtcgtgcc caagcacccc actcagttgt atgaggccct
     1501 ttcctacctc ctcctgtttg tgctgctgct gtacctgtac cgcaaaaaac tgcccaggct
     1561 gaaagaaggg actcttttcg ggttgttcct gctgctgctc tttgccgccc gcttctttat
     1621 tgaatacatc aaggaacccc aggtggcatt cgaagagact atggccctga atatgggaca
     1681 acttctgagt attcctttta tctttgcagg cgccatcctg ctgctataca gcatcatgcg
     1741 gggcaggccc gccatgcggg tcgagccggc agggaaacag aaggtgaaaa aacaggagaa
     1801 aaaaccaccg ctcagcagca cacgcggcac ctattgagcc atcgtacagc attatgccaa
     1861 aagcaaaatc cggggttgta atcaaaaaca agcgtgcctc tttcgaatat gaattcatag
     1921 acacgtatac ggccgggatt gtccttaccg gaacagagat caaatccatc cgggcaggaa
     1981 aagcctcgct cgtggattcc tattgttatt ttgaggggaa agagctgttt gtcaagaata
     2041 tgaacattgc cgattactgg tggggctcct acaacaggca tgaaccccgg cgggaccgga
     2101 aactgctact gacccggcac gaattgaaca aactgtaccg ggcaacgcgg gaaaaaggcc
     2161 ttaccatagt ggtcaccaaa ctattcatag ccgataacgg ctatgccaag ctcaatatcg
     2221 cactggcccg ggggaagagg gagtacgaca agcgtgaatc catccgggag aaggatcaga
     2281 aaagggactc ggaccgggct ttactcaacc gctccgggat aagggacaaa aaaggcgagg
     2341 gctaatacgc ccagtatgat gaccagcgaa atgaccagag gccataatat gcccatggat
     2401 tcaagtgcgg aaaaaagtcc gttttcaagg taatcaaggt accactgtcc gggtgttgaa
     2461 aggagagaaa tcattttttt ctgtattttt agtaaagata caattttagc ggataacgct
     2521 gcaacataca aggttcctgt caccatagcc attgtccacc cgcgtcacca ggggccagaa
     2581 tttgtgcatc acaatccagg gcagcgggta ggcagccttt tgacgcgcat agggatgagt
     2641 ccagttgtcc gagcagactt caacggccgt atggggggcg tttttgagca ggttgtcttc
     2701 tgctgtccca caggaatcat ctccggaggc caggcatttg cattcgtcca ggatggtcag
     2761 catggcctgg gcaaaacggt ccagttcttc ctttgactca ctttctgtgg gttctatcat
     2821 aagcgtttca tgcaccggga aagaaagggt gggcgcgtgg aagccgaaat ccatcaggcg
     2881 cttggccaca tcggtggcgt ctactccgta tgttttagaa aaatgacgca ggtctatgat
     2941 gcattcatgc gccacatagc cgtttctgcc tgtatagagg gttgcgtagt gcggggccag
     3001 gatacgggac aggtaattgg cgttgaggat ggccacttcc gaggcccggc gcaggccttc
     3061 tctgcccagc attttgatat acccgtaggt gatggggatg agcagggggc tgccataggg
     3121 cgaggcggcc acagggctgc cacccccgga attgaccagc ggatgaccgg gcaggtaccg
     3181 ggcgagttcc ctggtgcagc aaatggggcc tacgcccggg cctcctccgc catggggcat
     3241 ggcaaaggtt ttgtgcaggt tcaggtgaca tacatcggcg cctagcatcc cgggattggt
     3301 gatacccacc tgggcattca tattggctcc gtctatgtac accagtcctc cgttttcatg
     3361 tactatgcgg ctgatctcgc agatgccggc ttcatacaca ccgtgggtgg acggataggt
     3421 tatcatcata cacgagaggt tatcccggtg ttccagcgct ttctggcgca acgcttccac
     3481 atctatgttc ccttgatcgt cacatcccac gatcacgatt tccagaccgg ccatggcggc
     3541 gcttgccggg ttggtcccgt gagcagagga gggaatgagc gcaatgttcc tgtgtccttc
     3601 tcccctggcc cgctgaaagg cccggatgac catcagcccg gcatattccc cggcagcgcc
     3661 ggagttgggc tgcaatgagc aggcatccag cccggtgatg atggacaatt gttgttccac
     3721 ttcacggatc agctgcaggt acccttctgc ctgccaggag ggcgcaaaag gatgcacggc
     3781 cgtccattcc atctggctca ggggcatcat ttcggctgca gcattcagtt tcatggtgca
     3841 ggatcccagg gggatcatgg aatgggccag cgagatatcc cgtgtttcca gtgtcttgat
     3901 ataacgcatc atttccgttt ctgcatggta ggtgttgaac accggctctt caagtgcggg
     3961 agcggtcctt tccatgaagg acgggatttc cggcagggcg gtcttttctt ccaggggtat
     4021 attgaatatt tccaggatct tttccagttc ccgttcattg gtcagttcgt caaaggaaat
     4081 cctgacgctt tgcggatcgg gatagaagaa attgatctta agggcttccg cattggcctt
     4141 gatgcgagcc gtgtcgaccc cggtcagctc aatggtgtcg aaaaagtgtt tatgttttac
     4201 ggaaaacccg tattttgaaa ggatggcggc tgctgtatgg gcataatgcc ttccgttctg
     4261 tgctatccgg cgtatgcctt tgctgccgtg gtaaactgca tacattccgg ccatggtggc
     4321 catcagggcg gttgccgtac agatgtttga cgtggcgcgt tcccgcttga tgtgctgctc
     4381 gcgtgtttgc agggccagcc ggtatgccgg gtttcccagc ctgtccacag acaggcctac
     4441 aatgcgtccc ggaatgttgc gccggtattt gtccggacag gccaggtatc cggtagtcgg
     4501 acctccaaag gccatgggca atccaaagcg ctgtgcagtg ccggcggcaa tatcggcact
     4561 ccaggcggcc ggttcctgca gcacggccag ggacaaaagg tcacaaacag ccgttaccag
     4621 cagttccctt tcatgagcca ggcggcacag ggcactgtgg tcacacaact gtccgtctgc
     4681 gccggggtat tgtaccatca ccccgaacaa acggggtccg tgtttttcaa agacgggcag
     4741 cagttcccct gtcaggtctt ccgtgacaat ctcaatgccc aggcccagcg cacgggtttc
     4801 catcacggca agagtctggg gaaagatatt cctgtccacc agtatcacat tgcggccttc
     4861 ctttaccagt gtgcgggaac ggagttccag catcatgcgc atggcttctg cagcagccgt
     4921 ggcctcgtcc agcagcgaac tgttggccag gttgaacccg gtaaggctgc acaccatggt
     4981 ctggaaattg aacaaggctt ccaggcgccc ctgggaaatc tcggcctggt aaggggtata
     5041 ggatgtgtac cagcccggat tctcgaatat gttcctcaga atcaccggag gggaagcggt
     5101 tccgtaatag cccattccta tgagggagcg gaaaggcagg tttttggatg ccatggcttt
     5161 caggtgcatc agaaactcat attcgctcat tccttcttcc aggggaagcg ggtccttcat
     5221 ccttatctgt gccgggacgg tcttgtctat gagttcatcc agggagtcaa ccccaatgac
     5281 gctgagcatc tctttctgct gttcaggccg tggcccgttg tgtctttctg taaatgatag
     5341 tgccatatgt gatatattat tatctgatta atcgtttgaa gcgtccattt tcaacaggat
     5401 gaacaaaagt aatgtaaaag tccataagga ggaacccccg taactgataa aaggcaaggt
     5461 aatccctatg acgggcgtga ttccgatggt catcccgata ttgatgaata cgtggaaaaa
     5521 aatgcaggaa gcgaccccgt acccgtagat cctgcagaaa ggctgccgct gtctctcagc
     5581 tatccggatt atgcgtacaa gcaggaacgt gtagaggaaa atgaccaggg aagcccccaa
     5641 aaacccccat tcttccccta tggtgcagaa aatgaaatcg gtactctgtt ccggaacaaa
     5701 attgtatttg gtttgtgtcc cgtgaaggaa tccttttccg gcaaacccgc ccgacccgat
     5761 ggccactttg gactggtgca cattgtaacc cgttccataa atatccttgt caatccccag
     5821 gagggtttca atgcgtgcct gctggtgggg cttgagtatg ttgttgaata tgtaccccac
     5881 ggaaaagatc aggatgatac tgctcacaaa gcagagcagc aggattttca ggtaagcgat
     5941 ctttttacgc atggcaatga tgaaaagtac caccaccaaa acggccgcca atgccaggag
     6001 ccactgacag gcatccatat aggaaaagag gtcggctgag aaccagcgca ttaccagcgg
     6061 taccaggaac cataccgcgg caaaggagcc aaagattatg agcacgggca ttttcctgga
     6121 tgacaacagg agcgatatga taaaacagac gcccaggcaa agccagatga caaagagggg
     6181 gtcccagaca atggacagca agaataagat gatggccacg atgcctgtca cgggaatcca
     6241 cccgggcaat ccttcccggt ataatacaat aatgaaagca gacaggacaa gggccagccc
     6301 ggtttccttt tccagcagga tgagtgcggc ggggatgaca atcatggcag tgacctgcag
     6361 caagccgcgc catgaacgga tatggaaatg cgggcggctc ataacacccg caagacctat
     6421 ggcaacaaag acttttgtca tttccgcggg ctggaaatgt accggaccaa agacaagcca
     6481 ggaattggac ccgccccgtt ccacgccaaa gacaagcacc gcaagcagga gcaacaggcc
     6541 gatgatatag atcaggtttg agatcagggc atagacgcgg gcaggaacga gtaacaccac
     6601 aacagccagg aaccagcccc caagaatcca gatcatctgc aagccataac gttgtgatgt
     6661 gtcaaatata tgcgcgtgtt ccgggtcgta caccgcggca aatacactga tccatcctat
     6721 cagcacaaga accatgtagg tggcgacggt ccaccagtcc agttttctga tcaatttatc
     6781 gtttcttctc atcattgcag gtgacgtg
//
LOCUS       DAOSOF010000106         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_11777_length_6584_cov_5.893705, whole genome shotgun sequence.
ACCESSION   DAOSOF010000106 DAOSOF010000000
VERSION     DAOSOF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11777_length_6584_cov_5.893705"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLM68_06570"
     CDS             complement(<1..278)
                     /locus_tag="PLM68_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55059.1"
                     /translation="MEYLSGLTYDKTGCGYPMVMVLRVAGCSSANWYEKHEQEQGETR
                     GRKPSVCDQALLAAIREVIAESLFHGEGYIKVWKKLAKKKHIHVSNRRV"
     gene            complement(278..559)
                     /locus_tag="PLM68_06575"
     CDS             complement(278..559)
                     /locus_tag="PLM68_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55060.1"
                     /translation="MEKQKRKSANFKTNVVMELLRGETIEALSRKHGLTMAELSEWRD
                     AFIANGMDGFKKKPEEAKLARAERRIGQLEMELDLVKKKNEFIAKQRKS"
     gene            628..885
                     /locus_tag="PLM68_06580"
     CDS             628..885
                     /locus_tag="PLM68_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55061.1"
                     /translation="MEKTGVVLYNTDASEIGLNVFTDPRYDQFPKMKLACEKIAGEPS
                     GMTSFPYYETGSDKIINRKVFWETLTQEGNEWKFIWSGASE"
     gene            complement(860..1552)
                     /locus_tag="PLM68_06585"
     CDS             complement(860..1552)
                     /locus_tag="PLM68_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55062.1"
                     /translation="MNFDVHIFGEGFHRFVQQEMDWFTVPLGIIFHVLAFLVLLMVFI
                     YGNRARKVFTVYFALNWLFLFGFWGVYGFIYWAQIGPVYLATFIMTPILLGFIAAAWI
                     KECFNPSIDLDFRKVPVGRYMILLIVLWGFWYPTYIYGEGFTFSWRDLLLSNFGLMPC
                     PTTMVALGIFTLKYPAGNRRLFKLLTAFALVIGPPTVAAGWYPDIPLIMIGLYGLILI
                     ISYDFILMPRSR"
     gene            complement(1620..2219)
                     /gene="thpR"
                     /locus_tag="PLM68_06590"
     CDS             complement(1620..2219)
                     /gene="thpR"
                     /locus_tag="PLM68_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT55063.1"
                     /translation="MTSDLTGIRTFLALPLEPILPARERMQQLQKQLPSYRIKWVNSG
                     QFHLTLFFFGEIDAQIIPLLADRLQSEVTKIPCFTYSLGEPGIFSDRSGPRVLWLGIE
                     APSALYELKKAIDHAVAPLGFRSDDTAFHPHITLGRFLPGQKMTPSLYDILTETKSHQ
                     TSPLYSAKHLILFKSELFPAGPRYTPLARLDMDSQPPNS"
     gene            2304..3434
                     /locus_tag="PLM68_06595"
     CDS             2304..3434
                     /locus_tag="PLM68_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOT55064.1"
                     /translation="MNQDLIQSLFGSLALITWKQVVMLGIGSLLIYLAIAKKYEPNLL
                     LPMGFGTLLVNLPLSSAIGPDGALTVFFQAGIKTEVFPLLIFIAIGAMIDFSTLFRNP
                     LLLLFGAAAQFGIFFTIGVASLIGFDLKEAASIGIIGAADGPTSIYVASKFAEHLLGP
                     ISVAAYSYMALVPIIQPPVIKALTSKRERLIRMQGKQDPVSRTTLILFPIIITLIAGI
                     IAPSSIALIGFLMFGNLIRECGVLQKLSLAAQNELSSLVTILLGITISSTMTGEAFVN
                     LDTLFIIGLGLVAFIMDTAGGVLFAKLLNVFLPERLKINPMVGAAGISAFPMSARVIH
                     KMGQDEDKGNYLLMPAVSANVAGQIGSVIAGGLILTLVPYFL"
     gene            3453..3584
                     /locus_tag="PLM68_06600"
     CDS             3453..3584
                     /locus_tag="PLM68_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55065.1"
                     /translation="MNTFVSSLKIMGLGMLGIFVFMLLFGVVIYALHKIFPGTTAEK"
     gene            complement(3807..4769)
                     /locus_tag="PLM68_06605"
     CDS             complement(3807..4769)
                     /locus_tag="PLM68_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT55066.1"
                     /translation="MKNILVIGSVGQIGSELTLELRKKYGNGNVVAGYRSTLPKGELL
                     ESGPSERVDATDIQQIADVVKKYRIDTIFNLAAILSAVGESKPLTAWNIGIGGLMNCL
                     EVAREYGCAVSTPSSIGAFGGGTPLDNTPQDTIQRPQTMYGVSKVSGELLSDYYHKRF
                     GVDTRSVRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKKFTCPLKAGTFLDMMYMPDA
                     IRAAIDLMEADPTKLKHRNSFNITAMSFDPEIIYAEIKKHITDFTMTYQVDPVKQAIA
                     DSWPNKMDDSCARAEWGWKPEWDLPAMTRDMLRVIGEKHAKGLI"
     gene            4905..6095
                     /gene="kbl"
                     /locus_tag="PLM68_06610"
     CDS             4905..6095
                     /gene="kbl"
                     /locus_tag="PLM68_06610"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOT55067.1"
                     /translation="MYDRLQAHLQKELADIREAGLFKNERIIITPQGAEIKISTGATV
                     LNFCANNYLGLSNNPRLIAAAKKALDTHGYGLSSVRFICGTQDLHKELEAKIAEFFGT
                     EDTILYAACFDANGGVFEPLLTEEDAIISDALNHASIIDGVRLCKAVRYRYANADMAD
                     LEEQLKQAQAQRFRLIVTDGVFSMDGNVAPMDKIYQLAQKYDALIMVDECHSAGVVGE
                     TGRGVTEQFNLRGKIEIITGTLGKSFGGAIGGFTTGKKEIIGLLRQRSRPYLFSNSIP
                     PVVAAAGIEMFNMMSQTNELQDRLHENTKYFVQKMVDAGFDIKPTKSAICAVMLYDAK
                     LSQDMATRLLNEGIYVTGFYYPVVPKGQARIRVQISAAHKRPHLDKCIAAFTKVGKEL
                     GVIK"
BASE COUNT         1629 a         1672 c         1558 g         1725 t
ORIGIN      
        1 acacggcggt tggatacgtg gatatgtttc ttttttgcca gtttcttcca gactttgatg
       61 taaccctcgc cgtggaacaa gctctccgca atgacctctc tgatcgcagc aagaagggcc
      121 tgatcacaga ccgaaggttt ccgaccgcgt gtttcgccct gctcttgctc atgcttctca
      181 taccagttgg cgcttgaaca cccggcaaca cggagcacca tcaccatggg gtagccacag
      241 ccggtcttat cataggttag tccactcagg tattccacta gctctttctt tgtttggcaa
      301 tgaactcgtt cttttttttt accaggtcca actccatctc cagctgccct atacgtcgct
      361 cagctcttgc cagtttggcc tcttccggct tctttttgaa gccgtccatg ccgttggcta
      421 taaaagcatc tctccattcg gagagctcgg ccattgtcag accgtgtttg cggcttaatg
      481 cttcaatcgt ttcaccgcgt agcaactcca ttacaacatt tgtcttgaag ttggctgatt
      541 ttcgtttctg tttttccatt tgagtacaaa tttaacgttt tataccgcct tattttgtac
      601 tcggttcttt tagacactaa tatagtcatg gaaaaaacag gggtggtgct ttataacacc
      661 gatgcctctg aaatcgggct caatgttttt accgacccga ggtatgacca attcccaaag
      721 atgaaactcg cctgcgaaaa gatcgccggc gagccctccg gaatgacgtc attcccttac
      781 tacgagaccg gctctgacaa aatcataaac cgcaaggtct tttgggaaac ccttacccag
      841 gagggaaatg aatggaagtt tatctggagc ggggcatcag aataaagtca taagaaatta
      901 tcaggattaa tccataaaga ccaatcatga taaggggaat atcagggtac cagccggccg
      961 caacggttgg aggcccaatt accagggcaa aagcagttaa gagcttgaac aaccttctgt
     1021 tccccgcggg atattttaaa gtgaaaatcc ccagtgcaac cattgtagtg ggacatggca
     1081 tcagtccgaa attggataat aagagatccc gccagctgaa ggtaaagcct tcgccataaa
     1141 tatacgtggg ataccagaag ccccataata caatcagcag gatcatgtat cttcctacgg
     1201 gaactttcct aaaatccaga tcgatggacg gattaaaaca ttcttttatc catgctgccg
     1261 ctataaagcc cagcagaatt ggggtcataa taaaagttgc cagataaacc gggcctatct
     1321 gtgcccagta aataaacccg tatacacccc aaaagccgaa caaaaaaagc cagttgaggg
     1381 caaagtaaac cgtaaaaacc tttctggcgc ggttcccgta aatgaaaacc atcagcaaaa
     1441 ccaggaaggc cagtacgtga aaaataatac caagcggcac ggtaaaccag tccatttcct
     1501 gttggacaaa gcggtgaaat ccttcaccaa aaatatgtac gtcaaaattc attttttatt
     1561 tggccatagc ggttttttta aattcagcgg gcagattttc aagtagttcc ataattaatt
     1621 caggaattgg gtggttggga atccatgtcc aatcgggcca gcggtgtata acggggccct
     1681 gcagggaaga gttcgctttt gaacagaatc aggtgttttg cagaatataa cggagacgtc
     1741 tggtgtgatt tcgtttccgt aaggatgtcg tacagtgacg gtgtcatttt ttgtccgggc
     1801 aggaaacgtc ccaaggtgat atgcggatgg aaagcagtat cgtcggatct gaatccaagc
     1861 ggcgcaacgg catggtcaat agcctttttc aattcataaa gcgccgatgg cgcctcgatg
     1921 cccagccaaa gaacacgggg cccgctgcgg tcactgaaaa ttcccggctc tcctaatgaa
     1981 taggtgaaac agggaatttt agtgacttcg ctttgcagcc tgtctgccaa taagggtata
     2041 atctgagcat ctatttcacc aaaaaagaac aaagtcagat ggaactgtcc ggaattaacc
     2101 catttaatac ggtacgatgg caactgcttt tgcaattgtt gcattctttc ccgtgcagga
     2161 agtatgggct ccaggggcaa ggccaaaaaa gtgcgaatac cggtcaagtc agatgtcatt
     2221 ttcacattga ccaaatttac aaaaaaacga aagctgcaac ctcttttctt ttcttatatt
     2281 tgcacatcaa ctgaaacaac acaatgaatc aagatcttat acagtcactt ttcggcagcc
     2341 tggccctgat tacatggaag caggtggtta tgctgggtat cggctcccta ttgatctacc
     2401 tggccattgc caaaaagtat gaacccaacc tcctcttgcc catgggtttc ggcacccttc
     2461 tggtgaacct tccgctttca tctgcgatag ggcctgacgg tgccttgact gttttttttc
     2521 aggccgggat aaagaccgag gtattccctc tgctgatctt cattgccatt ggcgccatga
     2581 tcgatttcag cacgctgttc cgcaatccgc tcttgttgtt gtttggtgct gcggcgcaat
     2641 tcggcatatt tttcaccatc ggagtggctt ccctcatcgg atttgacctg aaggaagcag
     2701 cctccattgg aatcatcgga gcggcagacg gtcccacctc aatctatgtg gcctcgaaat
     2761 ttgccgagca cctgctcggg cctatatctg tggcggcgta ctcctatatg gccctggtcc
     2821 ccattattca gccgcctgtt atcaaggcac tgacttccaa gagagaacgg ttgatcagaa
     2881 tgcaggggaa acaggatcct gtctccagga ccaccctcat tctcttcccc ataataataa
     2941 ccctgatcgc gggaataatt gctccttcct ccattgcctt gatcggcttt ctgatgtttg
     3001 gcaacctcat ccgtgagtgc ggggtactgc agaagctgtc actggccgct cagaatgaac
     3061 tgtccagcct ggtaaccatt ctgctgggaa taaccatatc ctcaaccatg acgggtgagg
     3121 cctttgttaa cctggatacg ttgttcatta tcgggcttgg gctggttgcc tttatcatgg
     3181 atacggccgg aggggttctc tttgccaagc ttttgaacgt gtttttacct gaaagactaa
     3241 aaatcaatcc catggtagga gccgccggaa tatcggcttt ccccatgtct gcaagggtta
     3301 tccataaaat gggccaggat gaggacaagg gcaattacct gctcatgccg gctgtcagcg
     3361 ccaatgtggc cggacagata ggatcagtta tcgctggcgg tttaatcctc acccttgttc
     3421 cttatttttt gtaaattttg aatgaaatcg tcatgaatac ttttgtaagt tcactcaaaa
     3481 ttatgggact gggaatgctg ggcatatttg ttttcatgct gctctttggg gtcgtgatct
     3541 atgccctgca taagatcttt cccggcacca ctgcagagaa gtgaccactc ctcctacccc
     3601 tgttattccg catacaggtc actgtcacca caaagaacgg agaattttat tggtatgccg
     3661 aggttctccg ttctttgtgg tgcagcgacc tctcctgcaa ttagctgttc acctatgtag
     3721 ttactgcaat accgggtaaa ggaagcctcg gcttggagaa aaagcttcct ttacccggta
     3781 ttgcacgtga agagtgccgt ggatgtttag atcagtccct ttgcatgttt ctctccaatg
     3841 accctgagca tatcgcgggt catcgcagga aggtcccatt ccggtttcca tccccattct
     3901 gcccgggcgc agctgtcgtc catcttgttg ggccatgaat cggcgatggc ttgcttcaca
     3961 gggtccacct ggtaggtcat ggtgaaatcg gtgatgtgct ttttgatctc ggcataaatg
     4021 atctcgggat caaaactcat ggctgtgata ttgaaagagt tccggtgttt gagctttgtg
     4081 ggatcggcct ccataaggtc aatggcagcg cggatggcat cgggcatgta catcatgtcc
     4141 aggaaagttc cggctttgag tggacaggtg aattttttgt ttttgatggc ctcgtaatag
     4201 atctctacgg cataatctgt tgttccgcca ccaggcaagg taacgttgga tatgataccg
     4261 ggataacgaa cagaacgcgt atccacgcca aaacgtttgt gataatagtc actcagcagt
     4321 tctccagata ctttggaaac cccgtacatg gtctgcggcc gctggatggt gtcctggggc
     4381 gtgttgtcca agggggtgcc tccgccaaaa gcgccaatgg aactgggtgt tgaaacggca
     4441 caaccgtatt cacgggcaac ttccaggcag ttcatgagcc cgccaatgcc aatattccag
     4501 gcggtcaggg gtttgctctc accaacggca gagaggatgg cagccaggtt gaagatggtg
     4561 tctatcctgt acttcttcac aacatcggct atctgctgta tatcggttgc atctaccctt
     4621 tcggaaggac ccgattcaag caactcgcct ttgggcaagg tcgatctgta gccggcaaca
     4681 acattgccgt tgccatattt tttccgaagt tccagggtca gttccgaccc gatctggccc
     4741 accgagccaa ttaccagaat atttttcatt cgtttgttga attagtttct ttattatttt
     4801 tgttaactga aagtcccgca aatatacgaa tctgtaatac aaaagtaata tctttgcatc
     4861 accttttaag gttaaaaaag tgtcaactta aaacaattaa tattatgtac gatcgtcttc
     4921 aggcccatct ccaaaaggaa ttagccgaca tccgggaagc cggacttttc aagaatgaaa
     4981 gaatcatcat cactccccag ggagccgaga tcaagatcag caccggtgcc acggtactga
     5041 atttctgcgc caacaattac ctgggtcttt ccaacaatcc aaggctcatt gccgctgcca
     5101 aaaaagccct cgacacacac ggctacgggc tatcttctgt tcgcttcatc tgcggcaccc
     5161 aggacctgca caaagaactg gaagccaaga ttgcagaatt cttcggcaca gaagacacca
     5221 tcctctatgc cgcctgcttt gatgccaacg gaggcgtttt tgagcccctg ctaacagaag
     5281 aggatgccat catctcggac gcactcaacc atgcctccat cattgacggc gtaagactct
     5341 gcaaagctgt tcgttaccgc tacgccaacg cagacatggc cgacctggaa gagcagctca
     5401 aacaggcaca ggcccagcgt ttcaggctga ttgtcaccga tggcgttttc tccatggacg
     5461 ggaacgtggc tccgatggac aaaatttacc agctggccca gaaatacgat gccctgatca
     5521 tggtggacga gtgccactcg gcaggcgttg tcggggaaac aggccgaggc gtgaccgagc
     5581 agttcaacct ccggggcaag atcgagatca taaccggtac actgggcaaa tcttttggcg
     5641 gggccattgg cggattcacc accggcaaga aggaaatcat tggcttgctg cgccagcgct
     5701 cgcgtcctta cctcttctca aactccattc ctcccgttgt ggccgcagcc ggcattgaaa
     5761 tgttcaacat gatgtcccag accaacgagc ttcaggaccg cctgcatgaa aacaccaaat
     5821 attttgtaca gaaaatggtg gacgccggtt ttgacatcaa gcccaccaag agcgccatat
     5881 gtgccgtgat gctgtatgat gccaaattaa gccaggacat ggctacccgt ctgctgaacg
     5941 aaggcatcta cgttaccggt ttctactacc cggtagttcc caagggacag gcccgtatcc
     6001 gcgtacagat ctctgccgcc cacaagcgcc cgcacctgga caaatgcatt gccgccttca
     6061 ccaaagtagg caaagaactg ggcgtaataa aataaattcc gcactctgaa caacagagct
     6121 gcttttagtg tcgctacaaa aaaacgggga gctttccggg tacgccgggg ctctccgttt
     6181 ttttttctgt ctgctgcggt tcatgctttg ctgcctgttg cggttcatgc tttgctgcct
     6241 gttgcggtcg cagacaccac ttggaacgga aagcttgttt ggtacgccga ggcttcttct
     6301 tttctgtcac agcctggagc ggtcgctggc agcacctgga acggagcgct tgtttggtac
     6361 gccgaggcgc tccgttccag gtgctgtcag cgaccgcccg ccagacaaag gaggtggaac
     6421 ctcccgcgcc atttttgacg caaataaact gtaatcatct aatatacttt tgtttatata
     6481 atttttgtcc cgaatttggc gcatgaggtt ccacctcctt tgctcctgtc cctggatttc
     6541 gtttactgaa caagacttat catagaatta agcatactga acag
//
LOCUS       DAOSOF010000107         6573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_11803_length_6573_cov_4.735041, whole genome shotgun sequence.
ACCESSION   DAOSOF010000107 DAOSOF010000000
VERSION     DAOSOF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11803_length_6573_cov_4.735041"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PLM68_06615"
     CDS             complement(<1..1121)
                     /locus_tag="PLM68_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT55068.1"
                     /translation="MKKIILAGLLLAHLSLAAQEVRNLDSIVIVATQAAKDSPVAHSR
                     VEREDLLRTAPTQNLPFALAFTPGAVVVGENGTGSGYAYLRIRGSEGSRTQVNLNGIA
                     LNDAESQEVFWVNLPSLGGFLENVQVQRGVGTSVNGSGAFGATINMRTLYQSPGPYAQ
                     LQASYGSFNTWNFTVGAGTGTGSLFLPRGLAVDIRYAQNGTDGYIRNGYGNLHSLFTR
                     VSWIRPHYSLKLYYIYGEQHTGITWEGIPREKLAIDRRYNPAGEYTDSQGTVHYYPNE
                     TDNYTQHHIQLNHSRRFAHNISWSNTLHITKGDGYYENYKQKSSGDHIVRRSMDNIYT
                     AFSSIGSWTFTGAGKSAGSGEVLLGLNYAFYDGQHFGLIT"
     regulatory      complement(1109..1203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1202..1963)
                     /locus_tag="PLM68_06620"
     CDS             complement(1202..1963)
                     /locus_tag="PLM68_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOT55069.1"
                     /translation="MIRRLLIHSFLLLTTIGAAASPVPRYGYKIEDVMPHNISAYTQG
                     FFFHEGHFYESTGQYGSSMIAKVDMVTGKVLQSNALERKYFGEGACIHNGFIYQITWT
                     ERTCFVYDAKTFQLLGTRRYNREGWGLTTNGKELIMSDGSSTLYFLDPETLLDKSSVK
                     VTLDGREVNYLNELEYIDGEVWANIYTSEEIVRIDPATGKVTGVIDLKGILPVSLRRR
                     DTDVLNGIAYDPGTKSIWITGKNWPRVYKIAIEKH"
     gene            complement(1960..3582)
                     /locus_tag="PLM68_06625"
     CDS             complement(1960..3582)
                     /locus_tag="PLM68_06625"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT55070.1"
                     /translation="MPVKYVFVTGGVTSSLGKGTIAASLAKLLQARGLRVTIQKFDPY
                     INVDPGTLDPYEHGECYVTEDGAETDLDLGHYERFLGVYTTKSSNVTTGKIYQTIIDK
                     EREGAYLGKTVQVIPHITDEIKRRMLLLGKTGKYDIIITEIGGTVGDIESLPFVEAVR
                     QLQWELPEENCAVIHLTLVPYLAAAKELKTKPTQHSVRQLQQEGVNPDVIVCRTERPL
                     PYALRKKVALFCNVRPHAVIESLDQKSIYEVPLNLKRQNLDGILLDLLKIDNKVEPNL
                     DAWKQFIDRVYHPQKKIDIALVGKYVELQDAYKSILESFIHAGAANNTRVKVHSIQSE
                     HINQCNVEEKLAGMKGILVAPGFGPRGTDGKIFACKYAREHKIPFLGICLGMQMAVLE
                     FARNVAGIPGAESVEVAPKTEYPVIDLMEDQKRLTSKGGTMRLGAYECKLERGSLAYK
                     IYGKPLIQERHRHRYELNDCYLETLEKAGMKATGKNPETGLIEIMEIPNHPFYIGVQF
                     HPEFKSTPEKPAPLFRSFIAAAIRYYESLNTK"
     gene            3637..3971
                     /gene="rnpB"
                     /locus_tag="PLM68_06630"
     ncRNA           3637..3971
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM68_06630"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3973..4878)
                     /locus_tag="PLM68_06635"
     CDS             complement(3973..4878)
                     /locus_tag="PLM68_06635"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOT55071.1"
                     /translation="MKTIRTISLLIALLLAGGCGPMINILEVDVRQPASRPMNIMGRE
                     MAVFTPLYDSITLSDSVLMNRYAQSLADGLALQARLPKGAVPLFIHHTGSESLGTLDQ
                     PDYAHRLALETETDLLFMVDSVTIGGFLVNDNAYLLAPIQAVVRVYDAELVRFTRYIP
                     IRDTAAWDIGDQADKETITIPRSAFIDLQRAAVYMGEQTARSFMDQWETQDRVVFVFD
                     KLKWNRAYDHALAFEWDKAMEIWLELLKSNNTKEVACAAFNVALACEMQGNFALAEKW
                     LDLSQKTLDMAETRYYRDMLQERKK"
     gene            complement(4896..5642)
                     /gene="fabG"
                     /locus_tag="PLM68_06640"
     CDS             complement(4896..5642)
                     /gene="fabG"
                     /locus_tag="PLM68_06640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT55072.1"
                     /translation="MQLLKDKKVLITGAARGIGKAVALGMAGQGADVAFTDLIIDDKA
                     KETIKEIEALGVRAVAYASDASSFEQAHQTVSQVVQELGRIDILVNNAGITRDTLILR
                     MSEQQWDTVIQVNLKSAFNYIHAVTPLMVKQKGGSIINMSSVVGLSGNAGQANYSASK
                     AGLVALTKSVAKELGPRGIRANAIAPGFIISDMTDKLSEEVRKSWTDTIPLRRGGTPQ
                     EVANVCIFLASDLSSFVSGQVIPVCGGMYT"
     gene            complement(5659..>6573)
                     /locus_tag="PLM68_06645"
     CDS             complement(5659..>6573)
                     /locus_tag="PLM68_06645"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT55073.1"
                     /translation="AYLKLSEGCDRNCAYCAIPLIRGPHCSRPMSELLAEAGHLAGQG
                     VKELVLVAQDTSFYGLDLTGKRELATLMEGLCLIGGIERIRLLYTYPQGFPLDVLRLM
                     AQEPKICQYIDVPLQHVSDKVLTAMRRNTTKAETLQLIEDFRTFVPGLCLRTTLMVGH
                     PGEDEQAFLELLDFVKGARFERMGAFTYSREEGTYGALHLKDRVPEKIKRERYDRLME
                     LQQEISFTYNKSRIGSICSVLADYLVIPDEGPAKPVVLARSQFEAPEVDGYVILENAP
                     DIKPGSFVKVRIKNADAYDLYAQLIEKQ"
BASE COUNT         1424 a         1689 c         1694 g         1766 t
ORIGIN      
        1 gtgatcagtc cgaagtgttg gccgtcatag aacgcataat tcagccccag caacacctcc
       61 ccgctccctg cactcttgcc cgccccggta aaagtccagc tgccgataga gctgaaggcc
      121 gtgtatatat tgtccatgga gcggcgcaca atgtggtcgc ctgaactttt ttgcttgtag
      181 ttttcataat acccgtctcc ttttgtaatg tgcagcgtgt tgctccagga aatattgtga
      241 gcaaaacgtc gggagtggtt cagctggatg tggtgctggg tgtagttgtc cgtttcgttg
      301 gggtaataat gtactgtccc ttgggaatct gtgtattccc cggccggatt gtaccgcctg
      361 tcaattgcca gcttctcacg cggaatccct tcccaggtaa tccccgtatg ctgctctccg
      421 taaatgtaat agagttttag cgaataatgg ggccggatcc aggaaacccg cgtaaagagc
      481 gagtgcaggt tgccgtaacc attgcgtatg tagccgtctg tcccgttctg cgcataacgg
      541 atatccacgg ccagtccccg gggcaggaat aaactgccgg ttcctgttcc tgcccccacc
      601 gtaaaattcc aggtgttgaa ggaaccgtag gaagcctgta gctgtgcata gggaccggga
      661 ctctggtaaa gtgtgcgcat gtttatggta gcgccgaagg ccccgctgcc gttgaccgaa
      721 gtgccaaccc cgcgctgtac ctggacgttt tccagaaaac cgcccaggga cggaaggttt
      781 acccaaaaca cttcctggga ctcggcatcg ttcaaagcga tcccgttcaa attcacctgg
      841 gtacgtgaac cttcggaacc acgtatgcgc aggtatgcgt atccgctgcc ggtcccgttc
      901 tctcccacaa ccacggcacc tggagtgaag gccagggcaa aaggcaggtt ttgtgtgggt
      961 gctgtccgta gcaggtcttc gcgttctaca cggctgtgag ccaccggaga atcttttgct
     1021 gcctgggtgg caacaatgac tatggaatcc agattcctta cttcttgtgc tgcaagtgac
     1081 agatgggcca gcagcaggcc cgctaaaatg atttttttca tgtctttcct ccgccggtat
     1141 tatccggatc aggtcctaag ggtgtgttct cagcctgcgt agcaagcacc cctgaacatt
     1201 gtcaatgctt ttctatggct atcttatata cacggggcca gttctttccc gttatccaaa
     1261 tgctttttgt gccgggatcg tatgcaatcc cgttgagtac gtccgtatcg cgcctgcgca
     1321 gtgatacagg caggataccc ttcaggtcaa tcaccccggt tacttttcca gtcgccggat
     1381 caatacgtac gatctcttcg ctggtatata tgttcgccca cacttcgcca tcaatgtatt
     1441 ccagctcgtt caggtaattg acttcccggc cgtcgagcgt gactttcacc gagcttttgt
     1501 ccaaaagggt ttccgggtcc aggaaataaa gcgtggagct cccgtcgctc atgatgagtt
     1561 cttttccgtt tgttgtcagt ccccacccct ccctgttgta tcgccttgtg ccgagcaatt
     1621 gaaaggtttt tgcatcgtat acaaaacaag tccgttcagt ccatgtgatc tggtagatga
     1681 acccgttgtg gatgcatgcc ccttctccaa aatacttgcg ttccagggcg tttgattgaa
     1741 ggaccttccc ggtgaccata tccaccttgg caatcatgga acttccgtac tgaccggtgc
     1801 tttcgtagaa atgaccttca tggaaaaaga aaccctgggt ataggcactg atattgtgcg
     1861 gcatgacgtc ctcaatctta tagccatacc ggggaaccgg agatgccgct gcaccgatgg
     1921 ttgtcagcag taaaaagcta tgtatgagga gtcgtctgat catttcgtgt ttagtgattc
     1981 gtaataccga atggcggctg ctatgaagga gcggaacagg ggcgccggct tttcaggtgt
     2041 gcttttgaat tcaggatgga attggacacc aatgtagaac ggatggttcg gaatttccat
     2101 gatctcgata agccctgtct cgggattttt acctgttgcc ttcataccgg ccttttccag
     2161 tgtttcaagg tagcaatcgt ttaattcgta acggtgacga tgcctttcct gtataagcgg
     2221 tttcccgtat atcttataag ccagggatcc gcgttccagt ttgcactcgt aagctcccag
     2281 tctcatggtg cctcctttac tggtgagccg tttctggtct tccatcaggt caattaccgg
     2341 gtattcggtt ttcggggcaa cctcaacgct ttctgcccct gggataccgg caacgtttcg
     2401 ggcaaattcc aggactgcca tctgcatgcc caggcatatg cccaggaagg gaattttgtg
     2461 ctctcttgca tatttacatg caaatatttt gccatcagtc ccgcggggcc cgaatccggg
     2521 ggccaccagg atacctttca tgcctgccaa tttctcttct acattgcact gattgatgtg
     2581 ttcgctttgt atggaatgca cctttacacg ggtgttgttg gcagctcccg catggatgaa
     2641 cgattccagg atggacttgt aggcatcctg aagttccaca tattttccta ccagggcaat
     2701 gtcaattttc ttttgcgggt ggtaaacgcg gtcaatgaat tgtttccagg catccaggtt
     2761 aggttccact ttattatcga tcttgaggag gtccagcaga attccgtcca ggttctgacg
     2821 tttgagattc aagggaactt cgtagatgct cttctggtca agggattcaa taactgcatg
     2881 gggacggaca ttgcaaaaca gggccacttt ctttcgcagg gcatacggaa gtggtctctc
     2941 cgtgcggcat acaattacat cgggattgac cccttcctgt tgcaactgcc ttaccgagtg
     3001 ctgggtgggt tttgttttca attcctttgc agcagcaagg tacgggacca gcgtcaggtg
     3061 gatcacggcg cagttctctt ccgggagttc ccattgtaac tgacgcacgg cttctacaaa
     3121 gggaagggat tctatatccc ccacggtacc accaatctcg gtgatgatga tgtcgtattt
     3181 tccggtcttg cccaacagaa gcatccggcg cttgatctca tcggtgatgt gcggaatgac
     3241 ctgaacggtc tttcccaggt atgcgccttc gcgctccttg tctatgatgg tctggtagat
     3301 ctttccggtg gtcacgttac tggacttggt ggtgtagaca cccagaaagc gttcatagtg
     3361 gcccaggtcc aggtcggtct cggcaccgtc ttccgtgacg tagcattccc cgtgttcgta
     3421 aggatccagg gtaccggggt ccacattgat ataggggtcg aatttttgta tggtgactct
     3481 taatcccctg gcttgcagca attttgcaag agaggcggca atcgttcctt ttcccaaaga
     3541 agaagtaaca cctccggtta cgaagacata ttttacgggc ataagacagc ttttttcaaa
     3601 ctgctaatgt aaggagtttt ttttaacttt gccattgcgg agggttgtcc catcgctctt
     3661 ttccgaaagg agaagggagg aaagtccggg caggaaaggg cgaaaccctg tggaaatcac
     3721 agctggccgt aaggtcaggg agcgcttgac agaaaataac cgctgctgtc cttcgggaca
     3781 tgcggtaagg gtgaaaaggc ggggtaagag cccaccgtaa cggccggcga cgaccgttga
     3841 cgcaggcacc ggtttcctgc aaggccaagt ataccggcaa tattagggct gcccgtccta
     3901 tgccgggggg taggctgctt agataaatga tgggagttgg acagaacccg gcttacagac
     3961 cctccgtttt ttttactttt tccgttcctg cagcatgtcc ctgtaataac gtgtctcggc
     4021 catgtccagt gtcttttggg ataaatcgag ccatttttcc gccaatgcaa agttgccctg
     4081 catttcacag gcaagggcaa cgttgaaagc agcacaggcc acttctttcg tgttattgga
     4141 tttgagcagc tccagccaga tctccattgc cttgtcccat tcgaatgcca gtgcgtgatc
     4201 gtatgccctg ttccatttca gcttgtcaaa aacaaaaacc accctgtcct gtgtctccca
     4261 ttgatccatg aatgaacggg cagtttgttc tcccatgtag acggctgctc tctgcaggtc
     4321 aataaaagca cttctgggaa tggtaatggt ttctttatcg gcttgatctc cgatatccca
     4381 cgcggcagtg tccctaatgg ggatatatcg cgtgaatcgg accagttctg cgtcataaac
     4441 gcggactact gcctgtatgg gcgccagtag gtaagcattg tcattgacca ggaagcctcc
     4501 tatggtcacg ctgtccacca tgaaaagcag gtctgtttcc gtttccaggg ccaggcggtg
     4561 tgcataatcg ggctgatcca gcgttccaag ggattctgat ccggtatgat gtatgaacag
     4621 ggggaccgct cccttgggca atcgtgcctg cagggcaagt ccgtctgcca gtgactgggc
     4681 gtaacggttc atcaacaccg agtcggaaag ggtaatggaa tcgtacaggg gcgtgaagac
     4741 cgccatttcc cgtcccatga tattcatggg ccggctggca ggctgcctca catccacttc
     4801 caggatgtta atcatgggac cgcatcctcc cgccagcagc agtgctatca gcaaacttat
     4861 cgtacgtatg gttttcatat gatcatattt tattttcatg tatacatccc gccgcatacg
     4921 gggatgacct gtccgctcac aaacgaagac aaatccgagg caaggaatat gcagacgttg
     4981 gccacctcct gaggagtgcc accgcggcgc agcggtatgg tatcagtcca ggatttgcgg
     5041 acctcttccg ataatttgtc ggtcatgtcg ctgattatga atccgggagc tatggcattg
     5101 gcacgaatgc ccctgggtcc gagttctttg gcaaccgatt tggtaagggc caccaaaccg
     5161 gctttggaag ccgaatagtt cgcctgtccc gcattaccgg acagccccac gactgaactc
     5221 atattgatta tgctgccgcc tttttgtttt accataagcg gagtgacagc atggatatag
     5281 ttgaatgcag atttgaggtt cacctgaatt accgtgtccc attgctgttc actcatgcgc
     5341 aggatcaggg tgtcacgggt aatgcccgca ttgttaacca ggatgtctat gcgtcccagt
     5401 tcctgcacta cctggctgac tgtctggtgt gcctgttcga aggaggacgc gtcagaggca
     5461 taagctacgg ctcttactcc taaagcttca atctctttaa tggtttcttt agccttgtca
     5521 tctatgataa gatcggtaaa ggctacgtct gcaccctgtc ccgccatgcc cagtgccacc
     5581 gctttgccta tgccgcgggc ggcacctgtt atcaacactt tcttgtcctt tagtaattgc
     5641 attatctgtg gatttaaatt attgtttttc aattaattgt gcatacaggt catatgcatc
     5701 ggcattcttt atccggactt tgacaaagga gcccggtttg atgtcggggg cattttccag
     5761 aatcacataa ccgtcaactt caggagcttc aaactgtgat ctggccaaaa cgacaggttt
     5821 tgccggtcct tcgtctggta tgacaagata atcagccaga acgctgcata tggaccctat
     5881 ccttgacttg ttatatgtaa aagatatctc ttgctgcagt tccataagcc tgtcgtaacg
     5941 ctcacgtttg atcttttcag gtacccggtc tttgagatgc aacgctccgt aggtgccttc
     6001 ttcgcgtgaa taggtaaaag cacccattct ttcaaaccgg gcccctttca caaagtcaag
     6061 caattcaagg aatgcctgtt cgtcttcccc ggggtgtccc accatcaggg tagtgcgcaa
     6121 acatagaccc ggtacgaatg tacgaaaatc ttctataagt tggagtgttt cggctttcgt
     6181 ggtattcctg cgcatagctg tcagcacctt atctgaaacg tgttgcagcg ggacatcaat
     6241 gtattgacag attttgggtt cctgcgccat aagccggagc acatccaggg gaaaaccctg
     6301 gggataggtg tatagcaggc gaatgcgctc aatgccgcct ataaggcata ggccttccat
     6361 gagcgtggcg agctcccttt tccctgtcag gtcgagtccg taaaagctgg tatcctgtgc
     6421 caccaggacc agttccttta ctccctgtcc ggccaggtgg ccggcctcgg caaggagttc
     6481 gctcatgggc cgggagcagt gcggaccgcg gataagggga atggcgcaat aagcacagtt
     6541 gcgatcgcaa ccttcgctaa gcttcaggta ggc
//
LOCUS       DAOSOF010000108         6515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_11925_length_6515_cov_5.438700, whole genome shotgun sequence.
ACCESSION   DAOSOF010000108 DAOSOF010000000
VERSION     DAOSOF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11925_length_6515_cov_5.438700"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLM68_06650"
     CDS             complement(<1..328)
                     /locus_tag="PLM68_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55074.1"
                     /translation="MTSFFLKNRSEVLVVQSEGSLSGAHLETLTWLFGGALPTGPDDP
                     FLKGWFKGPRREMVTPWSTVATEICLSMNIPGIIRIEEFFPVGEHDTVDAMLQEKIDQ
                     GVIPGVV"
     gene            complement(339..1100)
                     /gene="rhaD"
                     /locus_tag="PLM68_06655"
     CDS             complement(339..1100)
                     /gene="rhaD"
                     /locus_tag="PLM68_06655"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF002963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HOT55075.1"
                     /translation="MILKYNGPLQRQLNDIASVAEYMWQKGWAERNAGNITVNVTEFI
                     GDENKNLPSINKIPLYHTIPQLSGQYYYLTGAGSRMRDIARNPMDQGIITRVAQDGKS
                     IDVLADKNLPPTMELPAHMLMHQVFAQEAIQNGIPVSAGRRVVFHAHPTELIALSHVK
                     ALQSTRAITEALYEMHAEVKICVPNGLAWVPYEMPGSMELAKASIKDLKNFQIMLWER
                     HGVLGTGNNILEVFDAVDTLNTAARIYLYVNSLRS"
     gene            complement(1105..1644)
                     /locus_tag="PLM68_06660"
     CDS             complement(1105..1644)
                     /locus_tag="PLM68_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT55076.1"
                     /translation="MPSKNTLIVNLFGGPAAGKTTCAWEIASKLKKQRLVTEYVSEYA
                     KELVWDEKLDILDGSEPHQHHIFEVQTHRVERLVGKVDVVVTDATLLNSLVFGVNVSD
                     AFRSEIIDNFNRFNNFNLFIQRTSYFEQEGRIHNLAQSLELDEKIIDILAGNKIPFET
                     YSHRTLDLVIMKICLLVKS"
     gene            complement(1675..3819)
                     /locus_tag="PLM68_06665"
     CDS             complement(1675..3819)
                     /locus_tag="PLM68_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOT55077.1"
                     /translation="MIRRFTWFVLAVCFPAILSASDPYQGICVPFGGVKVVPRITERQ
                     EGKVLTLEHWFFNTASGPAYGSMGLVPSAGPLSLQEGTTPQTPGSLHIVTRNPDMDLM
                     VTATPRGAMHRYAFFTPSGEPQKEGRILLDLSGTGDNPGSGESPGTGDNPGSGESPVA
                     GDNPGSGILWAGLRVEDPNTVTGSIINRADKETRHTYFAIRFSRPVKRFFATDSTMAP
                     ENYPLMGGPGLDVVFVFDLVKGGQQFVGDGVLEIQIAFSSIDAMGALNNLKAEQDGKG
                     FETLLWEAGQLWEKELGVITIDEPRGNEVLESRRSLFYESLSRTMIHPALSHDADGRF
                     RGNDNNIHTSEYHVNYAALYQGSMVRDVLMTLIKPERSRQFVASGLAHYDRSVPKLLP
                     GEGVLDKAMSQLTDACIKGILPASLTPRLLEAMTNTMQTPFQGLLSTARETGFVPAGA
                     EPGSGSIRLTKDFSYANWCLMVMASGAGQTETVNRVRPFVTAYQWLIEPETGFARERN
                     RDLSWTAPADPSASSEPANPSACSEPADSLACRDALLFVPHNMADIVERTGGRKGFER
                     RLDKAESFQELPYLYTWTASPWKGQEKIRKMMDAYSLEDETPAWFVFSAMGLYPLCPG
                     TDQYVLGVPYFKRMTVRLEGGKVLTIEAPRLTEKNIYVKEVKWNGKVLDTSYITHGLL
                     LEGGTLEFSMTDHPVRGRSFRNEKLPYSYLKK"
     gene            complement(3816..4931)
                     /locus_tag="PLM68_06670"
     CDS             complement(3816..4931)
                     /locus_tag="PLM68_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT55078.1"
                     /translation="MSEVFFTDFKSRSGMSLTKKLELLVKAAGIERLDCKDKFTAIKI
                     HFGERGNLAFIRHNYASRMVELLRKMGARTFLTDTNTLYSGWRSNAVDHLNIAYLNGF
                     NPISVPGAPVIIADGLRGTDQVVIPIPRGEYCREALIGSAIAKADVLVTMTHFKGHEM
                     TGFGGALKNLGMGSASVGGKLQLHSDSKPVIERANCTGCRQCVMNCRQGAVSLDKEKI
                     AVINYELCVGCGQCIAVCQYDTAQAVKSSTAQGLTAKISEYAWAVLQGKEHFHVSFIM
                     DVSPLCDCWGMNDIPIIPDIGMLASFDPLALDQACFDLAKKSPAMPGSVADPDRTDAR
                     VGEDKFLYANPRTDGVFGLAHAEKMGIGSRSYTLIEI"
     gene            5006..5233
                     /locus_tag="PLM68_06675"
     CDS             5006..5233
                     /locus_tag="PLM68_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOT55079.1"
                     /translation="MKTAARCNTHFQADVIKGNLENEGFHPVVFDSSMNSILPLEAMS
                     SENKIVVNVPDKEYQAVKEYLDTRKNGSDQD"
     gene            complement(5549..6307)
                     /locus_tag="PLM68_06680"
     CDS             complement(5549..6307)
                     /locus_tag="PLM68_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55080.1"
                     /translation="MKRTILLLAICLVSNSCQINSSEEKSELIERQHVFNECLSAAHH
                     HSGLIRTFREQASPDLFNPNILVNHDGIVKEIDRISLSYLDANGIEYNESEFRALSHK
                     ILFDNIKTRSDQSVQISNEFLNDLMGVLDFDNIDQIENDVSQVLFSEKYVSTSDELFN
                     ICAVTAAVFVDSALYWDENINEWIDSQTDDVSTKCLDWDYLWSLIEPIIYADAEAAVA
                     AAYIGAWLGPLDAVVIAAAAAIASIFACMTSVAP"
BASE COUNT         1669 a         1602 c         1496 g         1748 t
ORIGIN      
        1 ctaccactcc gggaattacc ccctggtcga ttttttcctg tagcatggca tcgacagtgt
       61 cgtgttcccc taccgggaaa aactcctcta ttcggattat tcccgggatg ttcatggaaa
      121 gacagatctc ggtggctacc gtgctccagg gcgtgaccat ttcacggcgc ggacctttga
      181 accagccctt gagaaaaggg tcgtcaggac cggtcgggag ggctcccccg aacaaccagg
      241 tcagtgtttc cagatgggca cctgataatg atccttctga ctgtaccacc agtacctcag
      301 aacgattttt cagaaaaaaa gaggtcatag gtgctgactt aactgcgaag cgaattaacg
      361 tacaggtaga tccttgcagc cgtattgagc gtatccacgg catcgaacac ttccaggata
      421 ttgttgcctg ttcccagtac cccgtggcgt tcccacaaca ttatttggaa attcttgagg
      481 tctttaatac tggcttttgc cagttccatg gatccgggca tttcataggg aacccatgcc
      541 aatccgttgg gcacgcagat tttcacttct gcatgcattt catacaatgc ctcggttatg
      601 gcacgcgtgc tttgtaatgc cttgacatga gaaagggcta tcagctcagt gggatgcgca
      661 tggaaaacca cccttctgcc agctgatacg ggaatcccgt tctgtatggc ttcctgtgcg
      721 aaaacctggt gcatgagcat gtgagcaggc agctccatgg taggtggcaa attcttgtct
      781 gccagtacat caatgctttt accatcctgg gctacccggg taatgatccc ctggtccatg
      841 ggattcctgg caatatcacg catcctggag cctgcaccgg tcaggtaata atactgtcct
      901 gacagttgcg gaatcgtatg gtatagaggt atcttgttga tggaaggcag gtttttgttt
      961 tcatctccta tgaattcagt gacgttaacg gtgatgtttc cggcatttct ttctgcccag
     1021 cctttttgcc acatgtattc ggcaacactg gctatgtcat tgagttgccg ttgcaggggt
     1081 ccgttgtact tcagaatcat tttttcaact tttaactagt aaacaaattt tcattataac
     1141 caggtccaga gtcctgtgac tgtaggtttc aaaagggatc ttgttacctg ccaggatatc
     1201 aatgatcttt tcatccagtt caagggactg tgccaggttg tggatacggc cttcctgttc
     1261 aaaatatgat gttctttgaa taaacaggtt gaaattattg aaccggttga aattgtctat
     1321 gatctcgctg cggaaagcat ccgatacgtt tactccaaaa acaaggctgt tcagcagggt
     1381 ggcatctgtt accacaacgt caactttgcc aaccagcctt tccacacggt gcgtctgtac
     1441 ttcaaatatg tggtgctggt ggggctcgct gccatccaga atgtctaatt tttcatccca
     1501 taccagctct ttcgcgtatt ccgaaacgta ttccgttaca agccgttgct tttttaattt
     1561 acttgcaatc tcccaggcac aggtggtctt gcccgcggca ggacccccaa aaagattgac
     1621 aatcaaagtg tttttggatg gcattttatt gtatttcagg gcgcaacaaa tatactactt
     1681 ttttagatag gaatacggca atttttcatt ccggaaggac cttccccgga ccggatggtc
     1741 tgtcatgctg aattccagtg ttcccccctc aagtagcaat ccgtgggtaa tgtaagatgt
     1801 gtccaggacc ttgccgttcc atttgacttc ttttacatag atgttctttt ctgtaagacg
     1861 gggtgcttct atggtcagca ccttgccgcc ttccaggcgg acggtcattc ttttaaaata
     1921 aggcactccc aaaacatact ggtctgttcc gggacacaaa ggatacaggc ccatggccga
     1981 aaaaacgaac catgccggag tctcatcctc caggctgtaa gcgtccatca ttttccggat
     2041 cttttcctgt cctttccagg gagatgctgt ccatgtgtat aagtaaggaa gttcttggaa
     2101 agactctgcc ttgtccaacc gccgctcaaa gcctttcctg ccgcctgtcc tttcaacgat
     2161 atcggccata ttatgcggaa caaacagtaa ggcatcccgg caggccaggg aatctgccgg
     2221 ctcactgcag gcagagggat ttgccggctc actgcttgca gagggatctg ccggcgccgt
     2281 ccagctgagg tccctgttcc gctcccgggc aaaacccgtt tcgggttcta taagccattg
     2341 ataagctgtt acaaaaggac gcacccggtt tacggtttct gtttgtcctg caccggaagc
     2401 cattaccatc agacaccagt ttgcatagga aaaatctttg gtcagccgga tagatccaga
     2461 cccgggttca gctcctgcgg gcacgaatcc cgtttcccgg gccgtggata gcagcccctg
     2521 gaagggagtt tgcattgtat tggtcatggc ctccagcaag cggggtgtca gggaggccgg
     2581 caggataccc tttatacagg catctgtcag ctgcgacatg gctttatcca gtacgccttc
     2641 tcccggcaat aatttgggca cactcctgtc ataatgggcc aaacctgaag cgacgaattg
     2701 acggcttctt tcgggtttaa tcaatgtcat caggacatcc cggaccatgc tgccctgata
     2761 gagggcggca taattcacat gatactcaga ggtatgtatg ttgttatcgt tgccacggaa
     2821 cctgccatct gcatcatgtg ataaggcagg atggatcatg gtccgggaca gggactcgta
     2881 aaacaggctc ctgcgcgatt ccagcacttc gttcccccgg ggctcgtcaa tggtgatgac
     2941 tcccaattct ttttcccaaa gctgaccggc ttcccagaga agcgtttcaa aacccttccc
     3001 gtcctgctct gccttcaggt tattcaaggc tcccatggca tctatggagg aaaatgcgat
     3061 ctggatttcc aggaccccgt ctcccacaaa ttgctgtcca ccctttacca ggtcaaatac
     3121 gaatacaaca tccagaccgg gcccgcccat caggggataa ttttccggtg ccatggtact
     3181 gtcggttgca aaaaaacgct tcacgggccg tgagaaacga atggcaaagt atgtgtgccg
     3241 ggtttccttg tcggcgcggt ttatgatact gccggtaacg gtattcggat cttctaccct
     3301 cagccctgcc cacaggatcc ctgagccggg attatccccg gcgactggac tttctcctga
     3361 gccgggatta tccccggtgc ctggactttc tcctgagccg ggattatccc cggtgccgga
     3421 aagatccagt aggatcctgc cttctttttg aggttcaccg gatggggtaa aaaaggcata
     3481 gcggtgcatt gcacctcttg gcgtggctgt aaccataagg tccatatccg ggttcctggt
     3541 tactatgtgc aaagatcccg gagtctgggg tgtggttcct tcctgcaggg ataaaggccc
     3601 ggcagaagga accaatccca tgctgccgta tgcagggcct gaagccgtat taaaaaacca
     3661 gtgctcaaga gtcagcactt ttccttcctg acgttctgta atgcgcggca caacctttac
     3721 accaccaaaa ggcacgcaaa tcccctggta aggatcgctt gctgaaagaa tggccggaaa
     3781 acatacggcc aggacaaacc aggtaaaccg tcttatcata tttctatcag ggtataactg
     3841 cgtgatccga tccccatctt ttcggcatgg gcaagcccga ataccccgtc tgtacgcgga
     3901 tttgcgtaaa ggaacttatc ttctcccacc ctggcatccg ttctgtccgg atcggccaca
     3961 cttccgggca tagccgggga ctttttggcc aggtcgaaac aagcctggtc aagggccagc
     4021 ggatcaaacg aggccagcat gcctatatcc ggaattatgg ggatatcgtt catgccccag
     4081 caatcgcaca ggggcgacac atccatgatg aaactcacat gaaaatgctc tttcccctgc
     4141 aggaccgccc aggcatattc ggagatcttg gccgtcaggc cctgtgccgt agaagatttg
     4201 actgcctggg ccgtatcgta ctggcacacg gctatgcact gtccgcaacc gacacagagt
     4261 tcgtagttga tgactgcaat cttttcttta tcaagggaaa cagccccttg acggcaattc
     4321 atcacgcact ggcggcatcc cgtgcaattg gcccgttcta tgaccggttt ggaatccgag
     4381 tgaagttgca gttttcctcc cacactggcc gagcccatcc cgagattttt cagggcgcct
     4441 ccgaatcccg tcatttcatg gcctttgaaa tgcgtcatgg tcaccagcac atcggctttg
     4501 gcaatggccg agcctatgag ggcttccctg caatactccc ctcttggaat gggaatcacc
     4561 acctggtctg ttcctctgag cccatcggca ataatgaccg gagccccggg aacgcttatg
     4621 ggattgaatc cgttcaggta agctatgttc aaatggtcca cggcattgga acgccaaccg
     4681 ctgtacaggg tattggtatc ggtcagaaaa gtccgggcac ccatcttccg cagcaattcc
     4741 accatcctgg acgcataatt gtgacgtata aaagccaaat tcccgcgttc tccaaaatgg
     4801 atcttgatgg ccgtaaattt atccttgcaa tcaagccgct caattccggc ggccttgaca
     4861 agcagttcca gctttttggt caaactcatg ccggaacgtg atttgaaatc agtaaaaaaa
     4921 acttcactca tgactgtttg ttttccgcgg tctaagatag caagatttta cgtatcttta
     4981 ggaagtaaaa tcaacgtgtc tgatcatgaa aacagcagcc cggtgcaata cacatttcca
     5041 ggccgatgtc ataaaaggga acctggaaaa cgaaggattt catcccgtcg tttttgacag
     5101 cagcatgaat tccatactgc ccttggaagc tatgtcctcg gaaaacaaga tcgttgtcaa
     5161 cgttcccgac aaggaatacc aggcggtaaa ggagtacctg gacacccgga aaaacggatc
     5221 tgatcaggat tgaattattt tccctgccct gtccaccagc tccagtacgt ccaggcttct
     5281 ttggtgcgtg ttgacggcag attctttggc gccggatgca ataacgtcca ggaatattac
     5341 tcctgttttt tcttctcttt tctttcttta tgacgatcaa ataagtaaaa cactaagacc
     5401 ataagaagtg aaactatgac tgaagaaatc acatctcgcc aagcaggtct attggagaaa
     5461 agtatatccg atgtgagaaa aaaagaataa aaggcaaaga aaacgagatt gtattttatt
     5521 tttttcataa ccagtctgaa atatattttc atggagccac tgatgtcata caagcaaata
     5581 ttgaagcgat agcagcagct gctgcaataa caaccgcgtc aagtggtcct aaccatgcac
     5641 caatataagc agcagcgaca gcggcctctg catcagcata tattatgggt tcgattaaag
     5701 accaaagata atcccaatcg aggcattttg ttgagacatc atcagtttgc gaatctatcc
     5761 attcattgat gttttcatcc caatatagag ctgaatcaac aaaaacagca gcggtcacag
     5821 cgcatatgtt aaaaagttca tctgatgtgc taacgtattt ttctgaaaaa aggacttgag
     5881 atacatcgtt ttctatctga tcaatattat cgaaatcaag gacgcccatt aaatcattaa
     5941 gaaactcatt tgagatttga acagattgat cactccgggt tttaatatta tcgaataata
     6001 ttttatgtga aagagcccgg aattcacttt cattatattc aataccatta gcgtctaaat
     6061 aagataaact gattcgatca atctctttaa caatgccatc atgatttacc agaatatttg
     6121 ggttgaaaag atcaggtgat gcttgctcac gaaaagtacg tattaaacca ctatgatgat
     6181 gtgcagcaga caaacattcg ttaaaaacgt gttgtctttc aattaattct gacttttctt
     6241 ctgaagaatt tatttggcaa gaattagaga ctaggcagat agctaataac aaaatagttc
     6301 gtttcatttt aattgttatt ttttagaagt taataatgat gtagcataat cttaaaaatt
     6361 ttaattgtta ataaatgtcg tataggtcaa atagatggca cgagtatcat tggatgcaaa
     6421 aattggattc aaaaagggat gtcggaatat aataagacag aacgttaaca ggtgaatatt
     6481 taccgactga gtaaatatat taaaaaattg aattt
//
LOCUS       DAOSOF010000109         6464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12061_length_6464_cov_4.164456, whole genome shotgun sequence.
ACCESSION   DAOSOF010000109 DAOSOF010000000
VERSION     DAOSOF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12061_length_6464_cov_4.164456"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLM68_06685"
     CDS             complement(<1..529)
                     /locus_tag="PLM68_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT55081.1"
                     /translation="MRSVITLMVVVVLAGPLSLSCSKWNNDAPKDREDIVLTKVQKEI
                     LHNSNGFGIDLFREMCLELEGENVFISPLSASLAISSVTNGAAGKTLEDMKATLGFAT
                     YSPDQMNGFFKHLVPALKEVDNTVKLSIANAVWIEKTFPVKSPFIQSLQQYYNASVNL
                     LDFSDPKSPSVINQWC"
     gene            complement(645..803)
                     /locus_tag="PLM68_06690"
     CDS             complement(645..803)
                     /locus_tag="PLM68_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOT55082.1"
                     /translation="MKKYECDLCGYVYDPAEGDPDNGIAPGTPFEDLPEDWVCPLCGA
                     GKEDFTPL"
     gene            complement(815..2893)
                     /locus_tag="PLM68_06695"
                     /pseudo
     CDS             complement(815..2893)
                     /locus_tag="PLM68_06695"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
     gene            complement(2968..4524)
                     /locus_tag="PLM68_06700"
     CDS             complement(2968..4524)
                     /locus_tag="PLM68_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55083.1"
                     /translation="MKKVILITLGFLFGHMQEVAGQDLERIMWENPWQGGIVSAGMAF
                     SDRDYSDISLYTGYRTGEFRNVYDPASVNSYGISTRSYMKLGNTYLNGSFAYGYDHRK
                     NQRWLGILDPYRTPFMLADSVAGNYVLESYHMSAGMAIPVSGKWTLGWQLSYDAKNGA
                     KYRDLRNKDTYMDFIIAPSVIYAHGPARIGLHLYFRRITEQTDYTQVETSTNKVLFLF
                     SGMWFNTQQVFTSSAPGTRLLRDHLYGGGITLHYRPGKFSIYDHFSTGLREQSQREDV
                     VRARKYGDVREWTWQNTLMLYYGPRHRIKADVSWAGLRGFEPIQRQELNQLSRLWEWV
                     QYGTIPSFVQERTCVDINYSYRIARDENYDSWRITAGVSRNLFRQQWIKYPLAVQRSL
                     NLTQVYAQLAKSWSAKRWMFDLSPGISYGAGQLEETSPEELQETLTGNDMGFENPENF
                     RLTEPSGMENEFFTAKRIAAGITIRSTFIANRAKGLNLFAEAGYRYTRQLTATNKVFH
                     EAGLSIGLIF"
     gene            complement(4521..5705)
                     /locus_tag="PLM68_06705"
     CDS             complement(4521..5705)
                     /locus_tag="PLM68_06705"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HOT55084.1"
                     /translation="MKRPGKILPVLFAAILLPVLLQGCLEVEPAYSALSVTVQPPDEQ
                     TGIDLTQVKIRAQGVDEPVFYEMYPDASGKARLEVSPGRYTMLLSALYPEGFSVSASY
                     PEFLALPMETTDFTFTLDLALSGGIIFRQIYYAGSKTFEGANYTKDQFLELYNNTDQV
                     YYLDSLCVGALAPANSTVSNNPWMGSDTVGLFQMTWMIPGHGTDYPLAPGQSVVLALN
                     AIDHTARATSGLDLSHAHFGFYDEELPNHEKHPDVPALKRIFAGQGTAYSMSVHSPAP
                     VIFRPVMGVARWLADVESWQNYQPGSTSGMKYMHIHRSWILDGVECVQNPANALKRQP
                     TSVDAGFIWLQQGPNLGNAVVRKVQEVLPGGRIVYSDTNNSSADFLSDVPAEPILKPL
                     WP"
     gene            complement(5710..6432)
                     /locus_tag="PLM68_06710"
     CDS             complement(5710..6432)
                     /locus_tag="PLM68_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55085.1"
                     /translation="MVSSRPAQLAFPEIKSLHTTISVIGAYLNVLSQDESMGAITVPT
                     QVAGRPYPYAGFYAGGNKTSSGTRSERLNTNIRFITHLPSVGMVVTLTTQLVLMDRTS
                     YVCEWEDQVQTYYYDDKGSRYSGKWAYSDDKFIKKVNPLYIMDLSGNLIPFTQDMEND
                     VRFRELIGTTNKESFYLGSRYPVYGILNIRLTKEIGRWAAVSFYANNFLNLDQLVREK
                     ISGTAYSRNLPFYFGAEVRLTL"
BASE COUNT         1566 a         1624 c         1579 g         1695 t
ORIGIN      
        1 cacaccattg gttgatgaca gaaggggact tcgggtctga aaaatcaagc aggttcacgg
       61 aggcgttgta atactgctgc agggattgga tgaaaggact ctttacggga aaagtctttt
      121 caatccatac ggcattggca atactcagct taacggtgtt atcaacctcc ttaagggctg
      181 gtacaagatg tttgaagaat ccattcatct ggtccgggga gtatgtggca aaaccaaggg
      241 ttgccttcat gtcttcgagt gtcttgccgg cagccccgtt ggttacggac gatatggcca
      301 gagatgcgct caatggggat atgaagacat tttccccttc cagttccagg cacatctccc
      361 tgaaaagatc tatgccaaaa ccattgctgt tatgcagaat ttccttttgt acttttgtaa
      421 gtacgatatc ttccctgtct ttgggtgcgt cattgttcca tttggaacag gataatgaga
      481 gcggtcccgc aaggacgact acgaccatta gagtgataac agatctcata tgtttaggat
      541 tttccagtac atagttaaaa tgcaaaacat atacgaatac tgcaccgtct gatcaatagc
      601 catcagccgt cctgcggacg acatttatca aagaatatta tggcttacaa gggtgtgaaa
      661 tcttccttgc ctgcaccgca aagggggcaa acccaatctt cgggaagatc ttcaaaggga
      721 gttcccggag ctataccgtt gtcggggtct ccttcagcag ggtcgtatac atacccgcac
      781 aaatcgcatt cgtacttttt cataaataat agtattaaag gtaaaaagat atgtccattt
      841 cttttagcaa ataatgtgcc ttcgcgtact tctggatcac ccacagtgca tagcggatat
      901 ccagggtaac acatttgttc agtgtgggat gaaagaatga atcggctccc agggattcct
      961 tattcccgtc aaaagcaagg cctaccagcc gtcccctgct gtccaggaca ggtgatcctg
     1021 aatttccacc ggttatatca aggtcacaca ggaagttcac atgcatgtta ccggttttac
     1081 ggtctttcca tacgccccag tcttctgact ccagtacctg acggaagggt tcattgatcc
     1141 ggaattcgta atcgtacgga ttccatttat cccacagccc ctgtatggtg ctgtaccagt
     1201 tgtatgttat cccgtcacgg ggataaagag gtcctaccgt tccgaaggtg agcctcatgg
     1261 tcatattggc atcgggatac tgcggaatgt cctgttcttt tctgaaagta tacaaggcac
     1321 gggtgtactg cgtgtctgcg tgccttgcat ttattgttcc gagtaatcga ttttcttctt
     1381 cattgaatcg cggggtgtca taactgtggt tgagcagaca ggccggatcg gcatttatct
     1441 cgtctatcag atgcggggtg aggaaatagt cacgaaagct ttccctgctc cggaacaccg
     1501 agttgtcaaa aatgttttcg gcaagcctcg aggcatcccc gttgaagtgg tcataacgtg
     1561 atgacaggta ttcaccccag taccggcgcg gaacgttttc acaaaaatcc cttaaggcgc
     1621 tgacaaacca ttccttttca agtccgggat ggtacccgtt gtaataatcc atcatgcgtc
     1681 ggtaatagct gctgaattcg tccctggtac aatccaccgt gtctgtgccg ttacgcctga
     1741 cttctgccct gaggcccgcc acccttcttg ccatcagaag gaaccgggag gatatgatgg
     1801 tctgtttgta ccattcacgt gctgccgtca gctcttctgt aactgaataa gcatattcaa
     1861 ggtcttcaaa caatgtgccc cataattcac ttgtattggc agaggcactg atccactgcc
     1921 gcactttggc ctcttcttcc ctcctgttgt caacaaacct gtgacggata agactgagaa
     1981 cttctccctc ccgcaattcg gatacattgg ccagactaaa ataagtatcg gcatattgca
     2041 gacggacagc ggggtccgat tccatccatc tgttcatgat ttcaagatgg gtttttgagg
     2101 tacggaccac cacgggatat gtcaccgttt gtttttcata cagtccgaaa gaagaattat
     2161 aacggttcgt ctggaaagga taacccagcg ccatgacaaa atccccttcc ctgatgccct
     2221 tggcgctgat ggtcagttta gcgtcaggta ccaggggaac gttcgcagga tcatagtcgg
     2281 cgggagaacc gtcgggagcc gtgtatatcc tgtacagggc aaagtcacct ttgtgctggg
     2341 gccattccca gttatccgtt tcccctcagt aggcagcaat ggatacgggg ggagcaccca
     2401 ccaggcgtac atcgctgtac ttctggtagc aatacatgta ataacgggag ccgctccaca
     2461 tggaagcgca catacaatcg taacctgtca tctgccggta ttctttttca aggagcgcat
     2521 acattctcct gccggtcaag gtgtccctta gccggagaac cctgtccgtc acatcaaaca
     2581 ctttgcgaag aaagtagacg tttttgcccg ggacaggttt ttcttcagca gcattgcggg
     2641 cccagaatcc ttcttccagg taatttgtct cctgggtgct gaatgcatga atgtccgaat
     2701 aggcgcaatg gtggttggtt atgagcagcc cgttatcaga aattatcgat cctgtgcagc
     2761 caaagtccat agacacgact gcacttgaaa gcgtgatcct gtcattgtca aagatcatct
     2821 ccggatgaat cttcaggcct tttttctgca tctgtctcac caggttttgc tccatcaggt
     2881 ggacaagcca catgccttca tctgccctga gaggtgtgaa cataaggaaa agtggaacaa
     2941 catataggat aaaccttttc acggatatta aaaaatcagt ccaatactca atccggcctc
     3001 atgaaagacc ttgttggtag cggttagttg gcgtgtatag cggtatcccg cttctgcaaa
     3061 cagattcagc cctttagcgc gattggcaat aaaagtcgac ctgattgtta ttccggcagc
     3121 tatccgtttt gccgtaaaaa attcattttc cattccggaa ggttctgtca accggaaatt
     3181 ttcgggattt tcaaatccca tgtcatttcc ggtcagcgtt tcctgcaatt cttccggcga
     3241 ggtttcttcc agctgtcctg ctccatagga tattccgggg gacaaatcga acatccatct
     3301 tttcgctgac cagcttttgg ccagctgggc atagacctgt gtaaggttca gggaccgttg
     3361 tacggcaagc gggtatttaa tccactgttg acggaacagg ttccgggata cccccgctgt
     3421 tatcctccac gaatcatagt tctcatctct tgcaatcctg taagaataat ttatgtccac
     3481 gcatgtcctt tcctggacaa aagatgggat cgtgccatat tgcacccact cccaaaggcg
     3541 tgacaactgg ttcagttcct gtcgctggat gggctcaaaa cccctcaacc ctgcccatga
     3601 tacatcggct tttatacgat gccgtggccc gtaatacaac atcagggtat tttgccaggt
     3661 ccattccctg acatccccat acttgcgtgc acgtacaaca tcttcacgtt gtgactgctc
     3721 cctgagcccg gttgaaaaat gatcgtatat cgaaaatttt ccgggccggt aatgcaatgt
     3781 gatgcctccc ccatagagat gatccctgag caggcgcgtt cctggagccg aggatgtgaa
     3841 tacctgttgg gtgttgaacc acatgccgga gaataaaaac agcactttat tggtactggt
     3901 ttccacctgt gtataatctg tctgttctgt tatgcgtctg aaatacagat gtaacccaat
     3961 gcgagccggt ccatgtgcat agatcacaga cggtgcgata ataaagtcca tataggtatc
     4021 tttattcctc aggtcgcggt attttgcgcc attctttgca tcgtatgaaa gttgccatcc
     4081 cagggtccat ttaccagaga cgggaatggc catgcctgcg ctcatatgat atgattccag
     4141 tacataattc cctgctacgc tgtctgccag catgaaaggc gtcctgtagg gatcaaggat
     4201 ccccagccag cgctggttct ttctgtggtc atatccatag gcaaatgaac cgttcaggta
     4261 tgtgttcccg agcttcatgt aagaacgcgt gctgatcccg tacgagttga ctgaagcagg
     4321 atcgtacaca tttctgaatt caccggtccg gtatcctgta tacagggaga tgtcagaata
     4381 atccctgtca gaaaatgcca tacctgccga gacgatcccg ccctgccagg gattttccca
     4441 cataatcctt tccaggtcct ggcctgccac ctcttgcatg tgcccaaaaa ggaaacccag
     4501 tgttatgagt atgactttct tcatggccag agaggtttca ggatgggttc cgcaggtaca
     4561 tccgacagga aatccgcaga tgagttattg gtatcactat atacgatcct tcctccggga
     4621 aggacttcct ggaccttgcg cacaacagca ttccccaggt tgggaccctg ttggagccat
     4681 atgaagccag catccacgga ggttggctgg cgtttgagcg cattggccgg attctgcaca
     4741 cattccaccc cgtccagtat ccaggaacgg tgtatgtgca tatacttcat accactggtg
     4801 cttccgggct ggtaattctg ccatgattcc acatctgcca gccacctggc cactcccatc
     4861 acaggacgga aaattacagg ggcaggggaa tgcacgctca tgctgtaggc tgttccctga
     4921 ccggcaaaga tccttttgag ggcaggcaca tcggggtgct tttcgtgatt gggcagttct
     4981 tcgtcataaa agccgaaatg ggcatgggac aggtccagtc cgctcgtggc acgggccgtg
     5041 tggtcaattg cattcaaggc aagcaccacc gactgtcccg gggccagcgg gtaatcggtt
     5101 ccatggccgg ggatcatcca ggtcatctga aacaagccca ccgtatcgct tcccatccag
     5161 ggattattgg aaaccgtcga gttggctggt gccagagcgc cgacgcagag cgagtccagg
     5221 taatatacct gatcggtatt gttataaagt tccaggaact gatccttggt ataattggcg
     5281 ccctcaaagg tcttgctccc tgcataatag atctgcctga agatgattcc gccggaaagg
     5341 gcgagatcca gtgtaaaggt aaaatcagtc gtttccatgg gcagggccag gaattcggga
     5401 tacgatgcgc tgacagaaaa accttcggga tacaaggctg acaacagcat ggtgtacctt
     5461 ccagggctga cttcgagccg ggcttttccg gaagcatcgg gatacatttc gtaaaaaacc
     5521 ggttcgtcca caccctgcgc cctgattttc acctgtgtca ggtcaatgcc cgtctgttca
     5581 tcgggtggtt gcacagtaac agacagcgcg gaataagctg gttccacttc caggcatccc
     5641 tgaagaagta ccggcagcaa tattgctgca aacaggacag gcagtatttt tccgggtctt
     5701 ttcatgtggt cacaaggtta atctgacttc cgccccaaag tagaagggca ggtttcgcga
     5761 ataggctgtt ccggagatct tttctctgac aagctgatcc aggttcagga aattgtttgc
     5821 atagaaagac actgcggccc atctcccgat ttccttggtc agcctgatat tgagaatccc
     5881 atacacggga tagcgggatc ccaggtagaa ggattccttg ttggtggttc ctatcagctc
     5941 gcggaaacgg acatcgttct ccatatcctg ggtaaaagga atgaggttgc ctgaaaggtc
     6001 cattatatat aaaggattca cctttttgat gaacttgtca tcgctgtaag cccatttgcc
     6061 agaatagcgg gatcccttgt catcgtagta gtaggtctgt acctggtctt cccattcaca
     6121 aacatagctt gtcctgtcca tcagaacaag ctgtgtcgtc agggtcacca ccatccccac
     6181 agaaggaagg tgggtgatga atcggatgtt ggtattgagg cgttcgctcc tggtacccga
     6241 actagtcttg ttccccccgg cataaaaacc ggcatagggg tatggtcttc cggccacttg
     6301 agtgggaacc gtaatggcac ccatggattc atcctgggat aacacattca ggtaggcccc
     6361 gatgacagat atggtcgtat gcaggctttt gatctcggga aaggccagtt gcgcgggccg
     6421 cgacgagacc attcaaatgg aaaatccttt cgaacattga aaac
//
LOCUS       DAOSOF010000110         6318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12443_length_6318_cov_4.063548, whole genome shotgun sequence.
ACCESSION   DAOSOF010000110 DAOSOF010000000
VERSION     DAOSOF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12443_length_6318_cov_4.063548"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PLM68_06715"
     CDS             <1..1534
                     /locus_tag="PLM68_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55086.1"
                     /translation="AIAPCDGQYQPGRMRTPLTDVNYLTIQGSHDADVSSYQGMRQFN
                     RVHFSEGFDGFVAGIYIWAANHGQFNTKWGKTDYSSPHINFYNLGQLLSAEDQMTIAK
                     TYISAFLEVTLRDQPCPADREGQSNQVNQVDLTNLKDQKGQYNSAELKDLKNMFRDHR
                     YARHWLPETVYLSQFREPGVNYIGTFAEDLDLTTGTLPGSEITATDLSIWREQALPLA
                     WGDADTRSITLGWNTTESGATACDTTACDSAACDSTSSDAATCDTTACDSAACDSTSS
                     DAATSDTTACDSTACDSNAYDTTKCNTCQRDTLAPAWHISWPDGALKSQKQVAAQKQV
                     AGLTASAYPVLTFSLAAADEKANPPGKEEEEKKKEKEKEKEKEKEKEKAADTAAADTT
                     EVEKAAKEAAKEAAKEAEEEAEEELIDFTIRLTDRNGTILEFPLSDFSPVQPVLKRKL
                     TKLAFMQKQAKSETILQYYYFPLERYATANPDFDFAHIISLSFIFNITPQGVVVINNI
                     GM"
     gene            1694..3052
                     /locus_tag="PLM68_06720"
     CDS             1694..3052
                     /locus_tag="PLM68_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT55087.1"
                     /translation="MKRLSTILPVLLVAGCLLSCHNSQQVITFPEPPRAKGVTDVLEL
                     RCEPLDTVRVAFIGLGMRGSGAVHRYTFLEGVKVVALCDVVPENIERANKTLEERGFP
                     KAEAYTGPEDWKKICERPDVDLVYVCTHWDLHAPIGVYAMEQGKHVALEVPAALTIEE
                     CWQLVNTAEKTRRHCIQLENCNYDFFEMATHQMARQGLFGEIVHAEGAYIHDLRFLNF
                     DDTTGYWNMWRLEHNALENGNLYPTHGLGPIAHILDIHRGDRMEYLVSVSSAQFGMSA
                     YAKEKFGEDSDYAQREYLNGDMNTSVIKTAKGRTIMLQHDVTNPRPYSRIHMISGTKG
                     FAQKYPVPGIALEPDAHRFLAPDKLDSLLRLYEHPIVREVGQKAREVGGHGGMDFIMD
                     YRLVYCLRNGLPLDMDVYDAAEWSAIIELSRTSVANGGRPVAIPDFTRGAWDRLAGVT
                     YY"
     gene            3095..4723
                     /locus_tag="PLM68_06725"
     CDS             3095..4723
                     /locus_tag="PLM68_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HOT55088.1"
                     /translation="MRFLASPVRTAYTLVLFALAGIAVSCTPSIPEAEMPKIVENEGR
                     YTLLVDGEPFFMFGGQSANSSTWPKMLPGVWSTIESMHANTLEIPIYWEQFEPEQGKY
                     DYSMIQMVLDQARERDVRLVLLWFATWKNGSNHYMPQWMKNDPQKYPNLMNQEGRYVD
                     SPSPHATAAMEADAKAFTEFMRYLKQADPQHTVIMVQVQNEPGSWYSVRDYSPAAQQL
                     FEQPVPEALLQAEVLQQLNIPADAKGTWEEVFGKDADEYFQAWHVASYIGYVASAGKA
                     VNPLPMYVNVALRDPLSNPPATQYESGGATDNVIPIWKAAAPSIDLVAPDIYLRGDKT
                     VLKVIELYTRPDNALMVPETGDHAKYLYKVIEKGIGFAPFGIDGRRSAAADSDRPDPL
                     SQEYKLLAPMAGKLAQWNFEGRIQSVVEPDEGEQVQRINLGDWEAVITFGRGRRLDLL
                     QDQQPQNRTGKAMIIKLGENEFLAIGTTCRFTFNPIGKNEGKNWQYLKVMEGYYENGE
                     FQMTRILNGDQTDWGGPYVGETPTLLHFSLGVRE"
     gene            4929..>6318
                     /locus_tag="PLM68_06730"
     CDS             4929..>6318
                     /locus_tag="PLM68_06730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT55089.1"
                     /translation="MKKNIALLILLPAMMAWQCSSPAKDKLPQEFNHTRDLAQLGIDS
                     VKIASVDSLLQSFVDSNKVNCVTAFVAQKGNVIYHNAFGWKDVENQVPATTADYYMLF
                     SQTKAITTAAFMTLVEKGLVSVDDPVSKYFPGIPDQVVTAVNPDGTYETRPVATPMTF
                     AHLMSHSSGLGAGLVADMRRIEAEKAAAEKAEAASAMQKEPVEKTAAAPKDGETGTAA
                     PATETQPGPCGQHSYYVGENSFPTLEEAMLDLARYPLGFDPGTKWNYHVSTNMLAYMI
                     ELISGKSLREYVKETILDPLGMVNTDWYYTPDKQENFVKPYNLADGKLEPATMMNMFV
                     QGTFCANQTYCEGAIGLNGPIGDYARFCQMMLNKGTFNGRRILKAETIEAMTTINRLP
                     EVNSGGEGFRFGLGFELYNELKKPAPEVSNSAYAWGGMLGTEYIIDPENDLIVLYYLN
                     MWQRELTYPLFLS"
BASE COUNT         1655 a         1651 c         1681 g         1331 t
ORIGIN      
        1 tgcaatcgcc ccttgtgacg gacaatacca gcccggacgc atgcgcaccc ccctcaccga
       61 cgtgaattac ctgaccatcc agggctcgca cgatgccgat gtgtcgtcct accagggcat
      121 gcgccagttc aaccgggtac atttttcgga aggttttgac ggattcgtgg ccgggattta
      181 catatgggct gcaaatcacg gtcagttcaa cacaaaatgg ggcaagaccg attactcctc
      241 cccacacatc aatttttata acctgggtca gcttctgagt gcggaagacc agatgaccat
      301 tgccaaaacc tacatcagtg cattcctgga ggttacactc agggaccagc cgtgtccggc
      361 agatcgggag gggcagagca accaggttaa tcaagtggat ttgacgaacc tgaaagatca
      421 gaaaggccag tacaacagtg cggagctgaa agatctgaaa aacatgttca gggatcacag
      481 atacgcacgg cactggctgc ccgaaaccgt ttacctaagc caattccgtg agcccggagt
      541 caattacata ggcacctttg ccgaggacct ggacctgaca acaggcaccc tgcccgggag
      601 cgagatcacc gccacagatc tgagcatctg gagggagcag gccctgcccc tggcatgggg
      661 cgatgccgac acccgctcca tcaccctggg ctggaacacc accgaatccg gcgcaactgc
      721 atgtgacacc actgcctgcg actctgctgc atgtgattcc acttcttccg acgctgctac
      781 atgtgacacc actgcctgcg actctgctgc atgtgattcc acttcttccg acgctgctac
      841 aagtgatacc actgcctgcg actctactgc atgtgactcc aatgcgtatg acactacaaa
      901 atgcaatacc tgccaacgtg atacactggc ccctgcatgg cacatcagct ggcccgacgg
      961 cgccctaaaa tcacagaagc aggttgccgc acagaagcag gttgccggac tcaccgcttc
     1021 ggcatacccc gttctgacat tctctctggc tgccgctgac gaaaaagcca atccccccgg
     1081 taaggaagaa gaggagaaaa aaaaggaaaa ggaaaaggaa aaagaaaagg aaaaagaaaa
     1141 ggaaaaagcc gcagacacag cagccgcaga tacaactgag gtagaaaaag cagcaaaaga
     1201 agcagcaaaa gaagcagcaa aagaagcaga agaagaagca gaagaagagc tcattgactt
     1261 caccatacgg ctgacagacc gcaacggcac catcctggaa ttcccgctaa gtgacttttc
     1321 cccggtccag ccggtactaa agcgtaaact gaccaaactg gccttcatgc agaagcaggc
     1381 caaatccgaa accatcctcc agtactatta cttcccgctg gaacgctatg cgaccgccaa
     1441 tccggatttt gactttgcgc acatcatctc actcagcttt atcttcaata ttacgcctca
     1501 gggggtggtg gtgatcaata atattggaat gtagtgcaac gcacacattg ttcttttggt
     1561 tctttatcag ctacttacga gattcatacc cctgggggta tgaatctcat atcttattga
     1621 ttatcaatca aacgaacaat gtttgcgggg cactcatatt ttttgccgat atttgaaggt
     1681 aaatacctgg attatgaaaa gactatctac tatcttaccg gtactccttg tggcggggtg
     1741 tctactatcg tgccataaca gccagcaggt gataactttt cccgaaccac cgcgtgcaaa
     1801 gggggtgacc gacgtgctgg aactgcggtg cgaaccgctg gacacggttc gcgtggcttt
     1861 tattggactg ggaatgaggg gatccggggc tgttcacagg tatacttttc tggaaggggt
     1921 aaaggtggtg gcactatgcg atgtggtacc tgaaaatata gagcgtgcca acaagaccct
     1981 ggaagagcgg ggattcccaa aagccgaggc ttataccggt ccggaggact ggaagaaaat
     2041 atgcgagcgg cccgatgtgg acctggtgta cgtgtgcacc cactgggatt tgcatgcacc
     2101 catcggcgta tatgccatgg aacagggaaa gcatgtggca ctggaggtgc ccgctgcgct
     2161 gactattgaa gaatgctggc aactggtcaa tacggccgag aagacgcgcc gtcactgcat
     2221 ccagctggag aactgcaatt acgacttttt cgagatggcc acgcaccaga tggcccggca
     2281 gggcttattc ggggaaatcg tgcatgccga aggagcgtac attcatgatc tacggttttt
     2341 gaatttcgat gatactaccg ggtactggaa catgtggcgg ctggagcaca acgcgctgga
     2401 gaacggaaac ctgtacccga cgcacggcct cggccccatt gcccacattc tggatatcca
     2461 ccggggggac aggatggaat acctggtgtc ggtttcctcg gcccagttcg gaatgagcgc
     2521 gtatgcgaag gaaaagttcg gggaagattc ggattatgcc caaagggagt acctgaacgg
     2581 cgacatgaac acctcggtca ttaaaacggc caaaggccgc accatcatgc tgcagcacga
     2641 tgtgaccaac ccgaggccct acagccgcat ccatatgatc agcggaacta agggttttgc
     2701 gcaaaaatac ccggtacccg ggattgctct ggaaccagat gcccatcgat ttctggcacc
     2761 ggacaaactg gattcgctgt tgcggctcta cgaacatccc attgtcaggg aagtgggaca
     2821 gaaggcccgg gaagtaggag ggcacggggg catggatttc atcatggatt accgcctggt
     2881 gtattgcctg cgcaacggcc tcccgctgga catggacgtt tacgatgccg ccgaatggag
     2941 cgccatcatt gaactttccc gcacctcggt ggccaatggc ggccgacctg tggccattcc
     3001 cgattttacc cgcggtgcct gggaccggtt ggcaggggtg acgtattatt aaagatttac
     3061 gattaattac cattaaccct ttaacatttt ttccatgaga tttttagcct ctcccgtacg
     3121 cactgcgtac accctggttt tgtttgcgct tgcaggtata gccgtttcct gcactccttc
     3181 gatccctgaa gcagaaatgc caaaaatagt tgaaaatgaa ggccgttata cccttttggt
     3241 tgacggggaa cctttcttca tgtttggcgg tcagtctgcc aattcttcca catggcccaa
     3301 aatgcttccc ggtgtgtgga gtaccattga gtccatgcat gccaacacgc tggaaattcc
     3361 catttactgg gaacagtttg aacccgagca aggcaaatat gattattcca tgatacaaat
     3421 ggtgctggat caggccaggg aaagggatgt ccgcctggta ttgctgtggt ttgccacctg
     3481 gaaaaacggg agcaaccatt acatgcccca atggatgaag aatgacccgc aaaagtatcc
     3541 caatctcatg aaccaggagg gccgctatgt ggactctccc tctccgcatg cgaccgctgc
     3601 catggaagcc gatgcaaagg cattcaccga attcatgagg tatcttaagc aggccgaccc
     3661 gcagcacaca gttatcatgg tgcaggtaca gaatgagccc ggatcgtggt acagtgtgcg
     3721 cgattattcg cccgccgcgc agcagctctt tgaacaacca gtacccgagg ctttgcttca
     3781 ggccgaggtg ttgcaacagc tgaacattcc tgccgatgcc aaaggaacgt gggaggaagt
     3841 cttcggcaag gatgccgatg agtattttca agcctggcat gtggccagct acattggata
     3901 tgtggcttcc gcagggaaag ccgtcaatcc gctgcccatg tatgtgaatg tggctttgcg
     3961 tgacccactc tccaatcccc cggcaacaca gtatgaaagc ggcggggcta cagataacgt
     4021 catccccata tggaaagcag ccgccccgtc catagacctg gtcgcgccgg atatctatct
     4081 gcgcggagac aagactgtct tgaaagtgat tgagttgtat acccgtcccg ataacgcgct
     4141 catggtgccg gaaacgggtg atcatgctaa gtacctgtac aaggtcatag aaaaaggcat
     4201 tggctttgcc ccgtttggaa tagacggacg ccggagcgca gcagcagaca gcgaccgtcc
     4261 cgatccactg tcccaggaat acaaattact tgctcccatg gccgggaagc tggcacaatg
     4321 gaactttgaa ggccgcattc agtctgtagt ggaacccgac gaaggcgaac aggtacaacg
     4381 cattaacctg ggagactggg aagccgtgat cacgttcggc agaggacgca ggctggacct
     4441 tcttcaggat cagcaacccc agaaccgcac cggtaaggcc atgatcatca aactgggtga
     4501 aaacgaattt ctggctattg gcactacctg ccgcttcacg ttcaatccca ttggaaagaa
     4561 cgaagggaaa aactggcaat acctgaaagt gatggagggt tactatgaaa acggtgagtt
     4621 ccagatgacc cgtatcctta acggagacca gaccgactgg ggcggaccct acgttggaga
     4681 aacccccaca ctcctgcact tctcgctagg tgtaagggag tagtcgctgc ccggcagcac
     4741 ccaagaagga ggtggaacct ccacccaggc aaacccggtt tgccgcgcaa gatgaatttt
     4801 gcattttgta gacaattgat attcagatgt ataaaatttc ttgaaaaagt gttgcgcggt
     4861 aatccgggtt tgccctaact tagaggccca aaacctgaat aattacctta ataattgcct
     4921 gagtatttat gaaaaagaac atcgcactgt taatcctgtt gccggccatg atggcctggc
     4981 aatgcagcag ccctgcaaag gacaaactgc cgcaagagtt taaccataca cgggacctgg
     5041 cccagttggg aattgattcg gtaaaaatag caagcgtaga ttcgcttttg cagtcgtttg
     5101 ttgacagtaa caaagtaaat tgtgtgacgg cctttgtggc tcagaaagga aacgtgatct
     5161 accacaacgc tttcggatgg aaggacgttg agaaccaggt ccctgccaca acagcagatt
     5221 attacatgtt gttctcgcag acaaaagcca ttacaaccgc tgcgttcatg accctggtag
     5281 agaaggggct ggtgtctgtt gacgatccgg tttcgaagta ttttccggga atcccggacc
     5341 aggtggtgac ggcagtcaat ccagatggga cctacgaaac ccgtccggtt gcaacaccca
     5401 tgacctttgc acacctgatg tcgcatagct cggggcttgg agcagggctg gtggctgata
     5461 tgcgccggat cgaagccgag aaggcagcag ccgaaaaggc cgaggcagcc tcggcaatgc
     5521 agaaggaacc agtagagaaa accgcagcag caccgaaaga tggagaaacc ggcacagcag
     5581 caccggctac cgaaacgcaa cccggtccct gcgggcagca cagttactat gtgggggaaa
     5641 acagcttccc gactttggaa gaggctatgc tggacctggc aagatacccg ctgggattcg
     5701 atccgggaac caagtggaat taccacgtga gcacaaacat gctggcctat atgatagagc
     5761 tgatttccgg gaaatccctg cgtgaatatg taaaggaaac catcctggat cccctgggca
     5821 tggtgaatac cgattggtat tacacgcccg acaagcagga aaactttgtc aagccctaca
     5881 acttggcaga cggcaaactg gagcctgcca ccatgatgaa catgtttgta cagggaacat
     5941 tttgcgcaaa ccagacatac tgtgagggag ccatcgggct gaacggtccc atcggggatt
     6001 acgcccggtt ttgccagatg atgctcaaca agggcacttt caacggccgt cggatcctga
     6061 aagccgagac cattgaggcc atgaccacga tcaaccgcct tcccgaggtg aactccggcg
     6121 gggagggatt ccgcttcggc ctcggctttg agctctataa tgaacttaag aagcccgctc
     6181 cggaggtgtc caacagtgca tatgcctggg gaggcatgtt gggtacggaa tacatcatag
     6241 atcctgagaa tgacctgatt gtgctctatt acctgaacat gtggcaacgc gaacttacgt
     6301 atccgttatt cttaagta
//
LOCUS       DAOSOF010000111         6309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12459_length_6309_cov_4.882475, whole genome shotgun sequence.
ACCESSION   DAOSOF010000111 DAOSOF010000000
VERSION     DAOSOF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_6309_cov_4.882475"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PLM68_06735"
     CDS             complement(<1..1029)
                     /locus_tag="PLM68_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT55090.1"
                     /translation="MTRRSGTIRLLLLAITFPVTVVAHAQESYNLRFVTLDSIVRTLE
                     LRTPERFYYAPEQTDTLLISLQADGKNVLEALGKELGKSGFSLYRMDQHGWAIIKGTP
                     VVEIHEAFYADSLENGKHQKEELSQLADNGRQAADSENRLYTLGDPRFSGLEGNVRLT
                     GYIHDHMSGEPVAGVSIMTEDKQYFAMSDSYGFYSLSLPRGYNTLLIQGFGYKDSRRY
                     IRIFSDSSLELNIAEEVFSLQGIVISAEKMENVRNVRLGIERVRADRIRHIPMAFGEA
                     DVIRAVLTLPGVKSVGEAAGGFNVRGGATDQNLILYNQGTIYNSSHLFGLFSLFNNDV
                     ISSLELYKR"
     gene            complement(1026..1751)
                     /locus_tag="PLM68_06740"
     CDS             complement(1026..1751)
                     /locus_tag="PLM68_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55091.1"
                     /translation="MTRSCSLPASLFILIVLSLPIEILKAQDYTPVSRYYVEQSGGYS
                     PLYNGKIPPMYNMLYEGTYFLESDAYYPGTVQYKGKLYVDLELNLNVHRDELYVRIPE
                     YNSGSVLLKAHVESFIMGDMQFINIVKEKYPAAPAEGYYRVLYSGDDISVLKRTTKRM
                     NSATIANDRKVTQVFEESISYYFLKNGYFHPVKNKSSVVRVLSGVRKELNRHIREDKL
                     RFGKDEREASLVSCAKLYQKEAK"
     gene            1824..3278
                     /locus_tag="PLM68_06745"
     CDS             1824..3278
                     /locus_tag="PLM68_06745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOT55092.1"
                     /translation="MKRIDCFIPAADYEQAEATLSHLRSLPLINRIFLLSAGQDTDLS
                     RTGTEVIRVKNLNSSDTVAAIARAATADYSLIYTKYTPLTPGYFALERFVQLGDDTRA
                     GLLYADHYQIINGQRRKMPLIECQEGALRDDFNFGSVLFYNTSVLKKAVAQTTDGYPY
                     AGMYNLRLAVSRMASLVHINEVLYTEEETDTRKSGEKLFDYVDPKNREVQVDMEKACT
                     EHLKAIGAYLPPCFTPVDLGRNDFEFEATVMIPVFNREKTIEDAVRSALAQKTSFKYN
                     VMVVNHHSSDKTVSIVEKLTDDPRLILITPERKDLFVGGLWNTALHHPKCGKFLVQLD
                     SDDVYSGQDSLSRIVEAFYQQQCAMIIGTYRMTDFGLNTLPPGIIDHREWTPENGRNN
                     ALRINGLGAPRAFYTPVLRELLLPNTNYGEDYGLGLRFSRHYQIGRIYDVLYCCRRWE
                     GNSDAALEPEKVNANNLYKDRLRTWEIQARKQLK"
     gene            3245..4552
                     /locus_tag="PLM68_06750"
     CDS             3245..4552
                     /locus_tag="PLM68_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT55093.1"
                     /translation="MGNTGKKTIEVGILLSPSISFELEGNYGAYSGRFTAIAENGQVR
                     LQDKVQDRFAFEPQDDNASFLLRDVVIGIEFHWERKEDQRFRGSLVLIPENNMVRAVN
                     VLTVEDYLISVIASEMSATSTLEYLKAHAVISRSWLLSQIEKRQGIAQQQTGACPSEV
                     RTLEEWIKWWDREDHTLFDVCADDHCQRYQGITRPSQSIDNVTQAVNQTAGEVLTFEG
                     EICDARFSKCCGGFMERFSTSWEPSEHPYLQGKYDGESFPAEVPFPDLSDPVQAEIWI
                     RSAPPAFCNTTDDEVLSQVLNTYDQETKGFYRWEVFYTFEELSDLIKRRIGVDFGLVQ
                     ELIPVERGSSGRICRLKIVGTKQTLIIGKDLLIRKALSESHLYSSAFVPVKRSTGFML
                     YGAGWGHGVGLCQIGGAVMASHGYTYKQILEHYYPGSRAQTIY"
     gene            4552..5853
                     /locus_tag="PLM68_06755"
     CDS             4552..5853
                     /locus_tag="PLM68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT55094.1"
                     /translation="MKEPSIGKKNAWAWIPTLYFAQGLPYVAVMTIAVIMYKRLGLSN
                     AEIALYTSWLYLPWVIKPLWSPFVDLVKTKRMWIILMQSFVAVGFAGVAFFIPASFFV
                     KATLAFFWLIAFSSATHDIAADGFYMLALEQGEQSFFVGIRNTFYRFATIFGQGILVM
                     LAGLLEEGKIFPATAGNIPLAWSLSFYLLAAIFLALALYHNFILPRPAIDKSRDNITP
                     LGLLKDFFLTFGSFFKKKDLGLIFFFILTYRLGESQLVKIASPFMLDQGEAGGLALPT
                     ATVGMVYGTIGVISLLVGGIIGGIVVSRGGLKKWIIPMALAINLPDLLYVYMASALPQ
                     NIWVIIASVAVEQLGYGFGFTAFMLYLIYVANGEHKTSHYAIGTGFMALGMMLPGMAA
                     GAIQEVLGYERFFWFVCICTLPGIFASWLVSRKLPADYGKK"
     gene            5867..>6309
                     /locus_tag="PLM68_06760"
     CDS             5867..>6309
                     /locus_tag="PLM68_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT55095.1"
                     /translation="MKNFIKGSLILVILLISLSCQSQERVVKTGIDVLKESNFSYLEG
                     KRVGLITNPTGVDRDLRSTIDILYEAPNVDLVALYGPEHGVRGDVHAGDQVSTQNDPA
                     TGLPVYSLYGSTRKPTPEMLQNIDVLVYDIQDIGSRSFTYISTMGL"
BASE COUNT         1584 a         1553 c         1573 g         1599 t
ORIGIN      
        1 tctcttgtat aattccaggc tgctgatgac atcgttgttg aaaagtgaga aaagcccgaa
       61 caggtgcgat gaattataaa tggtaccctg gttgtaaagg atcaggttct ggtctgttgc
      121 accacccctg acattgaatc cgcctgcagc ttcacccacc gatttgacgc ccggcagggt
      181 cagtacagcc ctgatcacgt cggcctcgcc gaaagccatc ggaatatggc ggatcctgtc
      241 ggccctgacc cgctctatcc ccaggcggac attccgcaca ttttccattt tttctgccga
      301 gataacaata ccctgcaacg aaaaaacctc ttctgcgatg ttcagctcca gggaactgtc
      361 ggaaaaaatc cggatgtaac gccgggagtc cttgtatccg aaaccttgta tcaggagtgt
      421 gttgtaaccc ctgggcagcg aaagggaata gaaaccgtag gaatcggaca tggcgaaata
      481 ttgcttgtcc tcagtcataa tgctgacccc ggcaacgggt tctccgctca tgtggtcgtg
      541 tatgtatccc gtcaggcgga catttccttc aaggccagaa aaccggggat ctcccagcgt
      601 ataaaggcgg ttttccgaat ctgccgcctg tcttccgtta tcagcaagct gcgacaattc
      661 ttccttctgg tgttttccat tctccaggga atcagcataa aaagcctcgt gtatttcaac
      721 cacaggggta ccctttatga tggcccaacc atgctgatcc atacggtaga gcgagaaacc
      781 gctttttccc agttccttgc caagcgcttc caggacattt ttgccgtcag cctgcaggga
      841 gatcaacaag gtatctgtct gttcaggtgc gtaatagaaa cgttcaggcg tacggagctc
      901 cagtgtccgt acaatggagt caagggtgac aaaacgcagg ttgtatgatt cctgtgcatg
      961 ggccacaacg gtgaccggga aggtaatggc taaaaggagt aggcggatgg tacccgagcg
     1021 tcttgtcatt ttgcctcctt ttggtatagc ttcgcacatg agaccaaaga ggcttcccgc
     1081 tcatcctttc cgaatctgag cttatcttcc cttatatggc ggttcaattc cttacgtacc
     1141 ccggacagaa ccctgacaac agaagattta ttcttcaccg gatgaaaata accgttttta
     1201 agaaagtagt acgaaatgct ttcttcaaat acttgagtaa ccttcctgtc attagctatg
     1261 gtagcactgt tcattctttt ggtggttctt ttgagcacac tgatatcgtc tccggaatat
     1321 agcacacgat agtacccttc ggcgggggca gcaggatatt tttctttcac aatattgatg
     1381 aactgcatat cgcccataat aaacgattct acatgagcct tcaacaagac agatccggaa
     1441 ttgtattcag gtatcctgac atacaattca tccctgtgta cgttcaggtt tagctcaagg
     1501 tcaacataca atttcccttt gtattggacg gttccggggt aatatgcatc tgattccagg
     1561 aagtaagtgc cttcatacag catgttgtac atgggaggta tttttccgtt gtacagcggt
     1621 gaatagccac cgctctgctc aacgtaataa cgtgaaacag gtgtgtagtc ctgggccttt
     1681 agaatctcaa tgggaagcga aagaactatc aggatgaaga gggaggccgg caaggaacag
     1741 gatcgggtca tgattacttt aattacagtc ggcaaaaata gcaaaaagat tgtacctttg
     1801 tcacactaaa actaacatta tacatgaaaa gaatagattg ctttatcccg gctgccgatt
     1861 atgagcaggc agaggccacc ttgtctcacc tgagatcact gccgctgatc aaccgcattt
     1921 ttcttctttc tgccggacag gatactgacc tttcacgtac cggaactgaa gtaatacggg
     1981 tgaaaaacct gaactcttct gacacggttg ctgccatagc ccgggcagcc acggcagatt
     2041 attctctgat atataccaag tacaccccac tgaccccggg ttattttgca ctggaacgtt
     2101 ttgtccaact gggagacgac accagggcag gattgctgta tgcagatcat taccagataa
     2161 ttaatggtca gcgaaggaag atgcctttga tcgaatgcca ggaaggggcg ttgcgggatg
     2221 atttcaactt tggttcggtg ttgttttaca atacctctgt cctgaaaaag gcagttgcac
     2281 agacaacaga tgggtaccct tatgccggca tgtacaatct caggctggca gtcagcagga
     2341 tggcttcttt ggtccacata aacgaagtcc tgtacacaga ggaggaaaca gatacaagaa
     2401 aatccgggga aaaactgttc gattatgttg acccaaagaa ccgtgaagtg caggtagaca
     2461 tggaaaaggc atgtaccgaa cacctcaaag caataggggc ttaccttccc ccgtgcttta
     2521 ccccggttga cctgggcagg aatgattttg aattcgaggc tacggtcatg atccctgttt
     2581 tcaacaggga gaaaaccata gaggacgccg ttcgttcggc cctggcgcag aaaacaagct
     2641 tcaaatacaa tgtgatggtc gtcaaccacc acagctcgga caagacggtg tccatagtag
     2701 aaaaattaac ggacgatccg cgcctgatcc tcatcacacc ggaaagaaag gatttgtttg
     2761 taggtggctt gtggaacaca gccctgcatc atcccaaatg cgggaagttc ctggtacagc
     2821 tggacagcga tgatgtatat tccggacagg attcactcag ccggatcgta gaggcatttt
     2881 accagcagca atgcgccatg attataggca catacagaat gaccgatttc ggactgaaca
     2941 cgcttccccc gggcataata gaccatcgtg aatggacacc tgaaaacgga aggaacaatg
     3001 cattacggat taacggactg ggcgccccgc gtgcttttta tacacccgtt ctgcgcgaac
     3061 tcctcctgcc caataccaac tacggagaag attacggact tggcctgcgt ttttccaggc
     3121 attaccagat aggtcgcata tacgatgtcc tttattgctg ccggcgctgg gaaggcaatt
     3181 cggatgcggc gcttgaaccg gaaaaagtga acgccaacaa cctatataaa gacagattgc
     3241 gcacatggga aatacaggca agaaaacaat tgaagtagga attctcctgt caccctcaat
     3301 ctctttcgag ctggaaggca attatggggc atattctggc agatttaccg ctatagctga
     3361 aaacgggcag gtccgtttgc aggacaaggt acaggacagg tttgcatttg aaccacagga
     3421 tgataacgcc tcttttcttc tgagagatgt ggtcatcggt atagagtttc actgggaacg
     3481 aaaggaagac cagcgttttc ggggttccct ggtgctgatc ccggaaaaca atatggtccg
     3541 ggccgtgaac gtgctgactg ttgaagacta cctgatcagt gtcatcgcct cggaaatgag
     3601 cgcgacaagc acccttgagt acctcaaggc ccatgccgtc atatcgcgca gctggctact
     3661 ctcacaaatt gaaaagagac aggggatagc acaacagcag acaggagcct gtccaagcga
     3721 agtccgtacc cttgaggaat ggataaaatg gtgggacagg gaggaccata cactttttga
     3781 tgtatgtgcc gatgaccact gccagcgtta ccagggtatc acccgtcctt cacaatccat
     3841 tgacaatgtt acacaggccg ttaaccagac ggccggcgag gtccttacct ttgaaggaga
     3901 aatatgcgat gcgcgctttt caaagtgctg cggtggcttc atggaacgtt tttccacttc
     3961 ctgggaaccg tccgagcacc cttacctgca gggaaaatac gatggagaat cgtttcccgc
     4021 cgaggtgcct tttccggacc tttccgatcc cgtacaggcc gagatctgga tccgcagcgc
     4081 accgcccgcc ttctgtaaca caacggatga cgaggtgctt tcccaggtgt tgaatactta
     4141 cgaccaggaa acaaaaggct tttaccgctg ggaagttttc tacactttcg aagagctttc
     4201 cgatctgatt aaaaggcgca ttggcgttga ctttggcctg gtgcaagaac ttattcccgt
     4261 ggaaagggga tcctcgggac gcatctgccg cctgaagatt gtgggaacaa aacagacgct
     4321 aatcatcggc aaggacctgc tcatccgcaa ggctctctca gaatcccacc tgtattcctc
     4381 ggcattcgta cctgtcaagc gttccacggg atttatgctg tacggggcag gctgggggca
     4441 tggagtggga ttgtgccaga ttggcggtgc cgttatggct tcacacggat atacctataa
     4501 acagatcctg gaacattact acccgggttc ccgggcacaa acaatctatt gatgaaagaa
     4561 ccttccattg gtaaaaagaa tgcctgggcg tggatcccca cgttgtactt tgcccagggg
     4621 ctgccctatg tggcggttat gaccattgcc gtgatcatgt acaaacgcct tggattatcc
     4681 aatgcagaga tcgcgttgta tacaagctgg ctgtaccttc cctgggtcat aaaacccctg
     4741 tggagccctt ttgtggattt ggtcaagaca aagcgaatgt ggatcattct gatgcagagc
     4801 tttgtggcag tcggtttcgc cggtgttgcc tttttcatac cggcctcctt ttttgtgaag
     4861 gccacgctgg cttttttctg gctcatagcc ttcagttcgg caacgcacga tatcgctgct
     4921 gacggttttt acatgctggc tctggaacaa ggggaacaat ccttttttgt agggatacgt
     4981 aatacttttt accgctttgc taccattttc gggcagggta tcctggtcat gttggccggc
     5041 ttgctggaag aaggcaagat attccctgca acagccggaa acatcccctt ggcctggagc
     5101 ctttcctttt acctcctggc tgctatcttc ctggcacttg ccctgtatca taattttatc
     5161 ctgccacgtc ctgcaatcga caagtccagg gacaacatca cccccctggg actgctaaag
     5221 gatttctttc ttactttcgg ttccttcttt aaaaagaaag acctgggact catctttttc
     5281 tttatcctga cctacaggct gggtgaatca cagctggtca aaattgcctc tccattcatg
     5341 ctcgaccaag gggaagcggg agggttggcc cttccaacgg ccacagtcgg gatggtatat
     5401 ggtaccattg gggtaatttc cctgcttgtg ggaggaatca taggggggat tgttgtttca
     5461 cgcggcggcc ttaagaaatg gatcataccc atggccctgg ccattaacct gccagacctg
     5521 ctctatgttt atatggcctc ggctctgcca cagaatatct gggtgattat tgcttccgtt
     5581 gctgtggaac agttggggta tggtttcggg tttacggctt tcatgctata cctgatctat
     5641 gttgccaacg gagaacataa aacatcgcat tatgccatag gaacaggctt tatggccctg
     5701 ggcatgatgt taccgggaat ggctgcgggc gccatacagg aagtactggg ttacgagcgg
     5761 tttttctggt ttgtatgtat atgcacgctc cccggcattt ttgcctcttg gctggttagc
     5821 aggaaacttc cggcagatta cggtaaaaaa taaaaaaact acggttatga aaaattttat
     5881 caaggggtcc ctaattctgg taatactcct gatttctctt tcctgtcaat cacaggaaag
     5941 agtggtcaag acaggcattg atgtgcttaa ggaatccaac ttttcctatc tggaaggcaa
     6001 acgggtggga ctgataacga atcctaccgg tgtggaccgg gacctgcggt ctaccataga
     6061 tattttgtac gaagcaccca acgttgacct ggtggccctg tacggacccg agcacggggt
     6121 acgcggggat gtgcatgccg gggatcaagt ctccacgcag aacgatccgg ccacagggct
     6181 tcccgtatat tcgctttatg gaagcacccg gaaacccacc cccgaaatgc ttcagaacat
     6241 agatgtgctg gtatacgata tccaggacat cggctcgcgt tccttcacct acatttccac
     6301 catgggact
//
LOCUS       DAOSOF010000112         6237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12646_length_6237_cov_5.666775, whole genome shotgun sequence.
ACCESSION   DAOSOF010000112 DAOSOF010000000
VERSION     DAOSOF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12646_length_6237_cov_5.666775"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..556
                     /locus_tag="PLM68_06765"
     CDS             95..556
                     /locus_tag="PLM68_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOT55096.1"
                     /translation="MSKYQIDATDHKILAFLVKNARMPFLEIARECGVSGAAIHQRVK
                     KMESMGIITGSRLLVKPQTLGLNVCAFVGIQLSQANQYPTVIEALKGISEVVECHFVT
                     GKYALWLKLYCRDHDHLMEVLIDIIRNIPSVIQTETLISLDQAIERQVWVK"
     gene            556..2097
                     /gene="purH"
                     /locus_tag="PLM68_06770"
     CDS             556..2097
                     /gene="purH"
                     /locus_tag="PLM68_06770"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT55097.1"
                     /translation="MTNNLRNIPIRKALISVYNKEGLAPVIHELVKAGVQLISTGGTL
                     EHIRSLGADATAVEDITGYPSILGGRVKTLHPKIFGGILGRRDVPGDITDMEHWEIGP
                     VDLVLVDLYPFSETVASGAPRADVIEKIDIGGISLIRAAAKNYHDVVVVADRSLYPEL
                     LDLLQSQQCHTTLDNRAYFAAQAFKITSHYDTAIFNYFNRDVKLTTFSHSLLETPLPL
                     RYGENPHQGAVFFGYQDSLPRKLHGKDISYNNLLDIEAAMRLIHDFSEPAVAILKHNN
                     ACGCATCPDPRQAWERALKADPASAFGGIIVMNRPLSDVLAQEMDKLFFEVIIAPSYG
                     PGALDVLTSRKNRIILAYGEKVRLPEKEFRSLLDGVVVQDRDTLVEDPASFRQVTRRA
                     PSPAQVADLVFANKLVKHTKSNAIVLAANGMLLASGMGQPSRVDALKHAIDKARENNL
                     SLENAVMASDAFFPFPDCVELAHKAGITAVIQPGGSIKDQLSIDYCNTNDMAMVFTGI
                     RHFKH"
     gene            2108..3127
                     /locus_tag="PLM68_06775"
     CDS             2108..3127
                     /locus_tag="PLM68_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT55098.1"
                     /translation="MAFSFLTQKLAIDLGTANTIIIHNDKIVVDEPSIVAIDQNTGKP
                     LAIGKTARLMQGKTHENIKTIRPLRDGVIADFNAAEQMVRGMIKMINPQNKFFMPSLR
                     MVVCIPSGSTEVEIRAVRDSSEHAGGRDVYMIYEPMAAALGIGIDVTAPEGNMVVDVG
                     GGTTEIAVIALGGIVCNQSIRVAGDVFTAEIQQYMRHQHNIKIGERTAEDIKICVGSA
                     LSDLDNPPEPMLVRGPNLMTALPVEVAVTSQEIAHCLDKSLVKIETAVLSVLEQTPPE
                     LYSDIVQRGIFLTGGGALLRGIDKRLQDKINIPFHISEGPLHSVARGTGIALKNIGKF
                     PFLLR"
     gene            3144..3980
                     /gene="mreC"
                     /locus_tag="PLM68_06780"
     CDS             3144..3980
                     /gene="mreC"
                     /locus_tag="PLM68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT55099.1"
                     /translation="MSGLPLLLRKLLVLLLFLALQTVAVVLLVNNSYFQQNAVMRVVR
                     SWQMHWWERTSSWTAFTNLRKINEDLFLENTRLRAEVARLSAFEENLLAETAGRMASA
                     DSFSFIPARVIRNSVNRQRNFLVIDKGSADGIAPDMGVISARGLVGVISHVSEHYAMI
                     ISLLNTDQRFTGVLEKTGAFGTLRWDGVNYKKITLTEIPRHIEVNPGDTVVSSHFSMI
                     FPPSMPIGRVLSSSLRQGTYLELEVELFSDFKTLRYVNVVSYSGAEELQRLTETEMEE
                     SL"
     gene            3977..4489
                     /gene="mreD"
                     /locus_tag="PLM68_06785"
     CDS             3977..4489
                     /gene="mreD"
                     /locus_tag="PLM68_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOT55100.1"
                     /translation="MNDNLKYILRFLFFLALQLLIFQNLNLGTYLAPMPYILFLMLFP
                     CTYPAALLMLWAFAFGLALDLLGTGIMGLHTSSLVLVGALRNILMKTVTVKGDLDNLM
                     VPGFSQFGFARYSIFISLCVMSHHLVFFALESLSLFLFWHTFARFLCSLLLNVFLILL
                     FKKTFFERGR"
     gene            4495..>6237
                     /locus_tag="PLM68_06790"
     CDS             4495..>6237
                     /locus_tag="PLM68_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT55101.1"
                     /translation="MANRERQKTIIIAFIVLCMVILAGRLVFVQLINKEYKITASNNV
                     LRYEIVYPARGLIYDRNGNILVESQFAYDIMITPRELEPFDTTGFCSIFNLTRERMDQ
                     MLSDIRGRRSAIGYQSVIFLRQVTKEQYTRFLEKSYKFPGFSVQTRSVRSYPSPICGN
                     LPGYIIEADPSFLEKNPEYRAGDYIGRTGLEEYYEKWLRGEKGYSIYLRDVHNRIKSA
                     YEDGAYDKTAVPGKNIITTIDLALQSFGEELMKNKVGSVVALDPATGEVLTLITSTGL
                     SSQYLTNFGSYYPQLLADPLKPLFNRAIMSSYPPGSVFKLVNGLIGLQEGVVKPETRY
                     SCSMGYHAGSLTVGCHSHPSPTNLLQSVMMSCNAYYCNVLRSILDNPAYGSIAEAMDK
                     WQAYVKSFGFGTRLGIDMPGELAGSLPGSALYDRIYGKNRWRSLTVISLSIGQGEIGA
                     TPLQLANLAAIMANRGHYYTPHLVREIPEDPSFRPNVERHQVMVDPENFRIIVEGMYQ
                     AVNAPAGSGATARQAAIPGVEMCGKTGTAQNPHGEDHSVFICFAPKDNPRIAVAVYIE
                     KGGFGASQAAPVASL"
BASE COUNT         1511 a         1663 c         1602 g         1461 t
ORIGIN      
        1 atggcatgca aaggggaaat ccccctgaaa tggttatttt actaccagaa gtctaataat
       61 ttttcataac tttacgtcca aacgtaaaat tatcatgtcc aaatatcaaa tcgatgccac
      121 agaccacaag atcttagctt ttctggtcaa gaacgcgcgt atgccttttc ttgaaatcgc
      181 ccgtgaatgc ggggtgtcag gagctgccat acatcagcgt gtgaaaaaaa tggaatccat
      241 gggaatcatc acaggttcac ggctgcttgt caaaccgcag accctgggtt tgaatgtatg
      301 tgcttttgtg ggcattcagc tgtcacaggc caatcagtac cccacggtaa ttgaagctct
      361 taaaggcatt tctgaagttg ttgaatgtca ttttgttact ggaaaatacg ccttatggct
      421 caagttatat tgccgcgacc atgatcattt gatggaggtc ctgattgaca ttatccggaa
      481 tatcccctcc gtcatacaga cagaaacttt gatttctttg gaccaggcga tagagcgtca
      541 ggtttgggtt aaataatgac aaacaatctg cggaacatac caattcgcaa agccctcatc
      601 tctgtataca acaaagaggg attggccccg gtgattcatg aactggtaaa ggccggcgtt
      661 caactgatat ctaccggagg caccctggaa cacatcagaa gcctgggtgc agacgccacg
      721 gctgttgaag acataacagg ttatccctcc atcctgggcg gacgcgtcaa aacgctgcac
      781 cccaaaatat tcggaggcat cctgggccga agggatgtgc ccggggacat caccgatatg
      841 gaacattggg aaatcggccc ggtggacctt gtgctggttg atctgtatcc tttcagtgaa
      901 acggtcgcca gtggcgctcc cagggccgac gtcatagaaa agatcgacat agggggaata
      961 tcgctcatcc gtgcagcagc aaagaattac cacgatgtgg tggtggtggc ggaccggtct
     1021 ctctaccccg agctcctgga cctgctgcaa tcgcagcaat gccatacaac gttggacaat
     1081 cgtgcgtatt ttgctgcaca agcattcaag atcacctccc actatgacac ggccatcttc
     1141 aattatttta accgggacgt gaaactgaca accttcagcc atagcctgct ggagaccccc
     1201 ctcccgttac gttacgggga aaacccgcac cagggcgctg tgtttttcgg gtatcaggac
     1261 agccttcccc gcaaactgca cgggaaagac atttcctaca acaacctgct ggacattgaa
     1321 gcagccatgc ggctgataca cgatttcagc gagcctgcag ttgccattct caagcataac
     1381 aatgcctgcg ggtgcgctac ttgcccggat ccccggcaag cctgggaacg cgccttgaag
     1441 gcagacccgg caagcgcctt tggagggatc atagtgatga accgtcccct ttcagatgtg
     1501 ctggcacagg aaatggacaa gcttttcttt gaagtgatca ttgccccttc ttacgggccc
     1561 ggagcgcttg acgtgcttac cagcaggaaa aaccgcatca tcctggcgta cggcgaaaag
     1621 gtccgattgc ctgaaaaaga gttccgctcc cttcttgacg gagtggttgt gcaggacagg
     1681 gacactctgg tggaagaccc agcctctttc agacaagtta cccgcagggc tccttccccg
     1741 gcacaggtgg ccgacctggt atttgccaac aaactggtaa agcataccaa atctaatgcc
     1801 atagtgctgg ctgccaacgg gatgttgctc gccagcggca tggggcaacc ctcccgggta
     1861 gacgccctga aacacgccat agacaaagcc cgggaaaaca acctgtccct tgaaaatgcg
     1921 gtcatggctt ccgacgcgtt tttccccttc cccgattgtg tagaactggc gcataaggca
     1981 ggcatcacgg ccgttatcca gccgggtggc tccattaagg accagttaag tatagattat
     2041 tgtaacacca acgacatggc tatggtcttt accggcatac gccatttcaa acattagcaa
     2101 caagcatatg gctttttcct ttttaactca aaaactcgcc attgacctgg gtactgccaa
     2161 taccatcatc atccataacg acaagattgt tgtggacgaa ccctccatag tggccatcga
     2221 tcagaatacg ggcaaaccgt tggctattgg aaaaacggca cgtctgatgc agggcaagac
     2281 acacgaaaac atcaagacca tcaggcccct gcgcgacggg gtgattgctg atttcaatgc
     2341 ggcggaacaa atggtacgcg gtatgatcaa gatgatcaac ccgcagaaca aattcttcat
     2401 gcccagcctg cggatggtcg tctgcattcc ctcggggagc acagaagtgg agatccgtgc
     2461 tgtccgcgac tcgtcggaac atgcgggggg ccgtgatgta tacatgatct acgaacccat
     2521 ggcagcagcg ctcggtatcg gcattgatgt gacggctcct gaaggcaaca tggtggtgga
     2581 tgtgggcgga ggaaccacag aaatcgccgt tatcgccctg ggaggcattg tctgcaacca
     2641 gagcatccgc gtggcgggtg acgtgttcac tgcggaaatc cagcaataca tgcgccacca
     2701 gcacaacatc aagattgggg aacgtaccgc cgaggatatc aagatatgtg tcggctcggc
     2761 cctctcggac ctggataacc cacccgaacc catgttggtg cggggtccca acctgatgac
     2821 ggctctcccc gtggaagtag cggtaacttc acaggaaatt gcccattgcc tggacaaatc
     2881 cctggtgaag atagaaacag ccgtgctatc cgtactggaa cagacgcctc ccgaattgta
     2941 ctcggacatc gtgcaacggg gcattttcct taccgggggc ggagcactgc tgcggggcat
     3001 tgacaaacgc ctgcaggaca aaataaacat cccgttccac atttcggaag gcccgctgca
     3061 ttcagtggcc aggggaacgg gtatagcctt gaagaatatt gggaaattcc catttttatt
     3121 aagataagat aacttgatcg tgcatgtccg gtctgcccct tttgctgagg aaactccttg
     3181 ttctgctgct gtttcttgca ttgcagactg tggctgtcgt gctcctggtc aataacagtt
     3241 atttccagca aaatgcggta atgcgggttg tccgttcctg gcagatgcac tggtgggaaa
     3301 ggacttcttc gtggacggct ttcacaaacc tgagaaaaat caacgaggac ctgttcctgg
     3361 aaaacaccag gctcagggcc gaggtggccc ggctttcagc atttgaggag aacctgctgg
     3421 cagaaacagc cgggaggatg gcatccgccg attccttctc tttcatacct gcccgggtca
     3481 tccgcaattc cgtgaaccgg cagcgcaact tcctggtcat agacaaggga tccgcagacg
     3541 gaattgcacc cgatatgggg gtgatcagcg cccggggact tgtaggggtg atctcccatg
     3601 tttcagaaca ttatgccatg atcatttcgt tgctcaatac agaccaacgg tttacaggag
     3661 tacttgaaaa gaccggcgct tttggaacat tgcgctggga tggagtgaat tataagaaga
     3721 tcaccctgac ggaaattcca cggcacattg aggttaatcc cggtgacacg gtggtaagca
     3781 gccatttttc catgatcttt cccccctcca tgcccattgg ccgggttctg tcatcaagcc
     3841 ttcgccaggg aacttacctg gaactggaag tggagctgtt ctcggatttc aagacgctgc
     3901 gttatgtgaa cgttgtgtcc tacagtggcg cggaagaatt gcagcgactt actgaaacgg
     3961 aaatggagga gtcgctatga atgacaacct gaaatacatc ctgcgcttcc tgttctttct
     4021 ggccctgcag ctgttgatat tccagaacct gaacctgggc acgtaccttg ctcccatgcc
     4081 ctatatcctg ttcctgatgc tttttccctg cacttatccc gccgcattgc taatgctctg
     4141 ggcttttgcc tttggcctgg cccttgactt gctgggaacg ggcatcatgg gcctgcacac
     4201 ctcgtcttta gtgttggtgg gggcgctgcg gaatatcctc atgaaaacag tgaccgtaaa
     4261 gggtgacctt gacaacctca tggtcccggg attcagccag ttcggttttg cccggtacag
     4321 catcttcata tccctctgcg tgatgtcaca ccatttggtg ttctttgccc tggaaagcct
     4381 gagccttttc cttttctggc atacgtttgc ccgtttttta tgcagcctgc ttctcaatgt
     4441 tttcctgatc ctgttgttta aaaaaacctt ttttgaacgg gggaggtgag cggtatggcg
     4501 aacagggaaa gacagaaaac catcatcatt gccttcatag tcctgtgcat ggtcatcctg
     4561 gcaggccgtc ttgtgttcgt tcagctcatc aacaaggaat ataaaatcac ggcgtccaac
     4621 aacgtgctcc ggtacgaaat cgtgtatccc gccaggggac ttatctacga caggaacggc
     4681 aatatcctgg ttgagagtca gttcgcttat gacatcatga ttacgccacg ggaactggag
     4741 cctttcgata ccacaggatt ctgctccatt ttcaacctga cgcgtgaacg catggaccag
     4801 atgctgtcag atatccgggg ccggcggagc gccataggat accagtccgt gatcttcctg
     4861 cggcaggtaa ccaaggaaca atacacccgt ttcctggaaa aatcgtacaa gtttcccggt
     4921 ttttccgtcc agacacgcag tgtgcgcagt tacccgtccc caatctgcgg caaccttccg
     4981 ggttacatca tagaagccga tccctctttc ctggaaaaga acccggaata ccgcgccgga
     5041 gactacatcg gacggacggg ccttgaagaa tattacgaaa aatggctccg cggggaaaaa
     5101 ggatattcca tttacctcag ggatgtccac aaccgcatca aatctgccta cgaggatggg
     5161 gcttatgaca aaaccgcggt ccccggaaag aatataatca ccaccattga ccttgcccta
     5221 caaagttttg gagaagaatt gatgaaaaac aaggtgggtt ctgtggtggc gcttgatcct
     5281 gctaccgggg aggtgctcac actgatcaca tctaccgggc tttcctcaca atatttgacc
     5341 aatttcggga gttactaccc gcagctcctt gcagacccgc tcaagcccct gttcaaccgg
     5401 gccatcatgt cttcttatcc tccaggatcc gtattcaagc ttgtaaacgg cctgattggg
     5461 ttgcaggaag gggtggtcaa accggagacg cgttattcct gcagtatggg gtatcacgcc
     5521 gggtccctaa cagtggggtg ccacagccat ccttccccca ccaacctgct gcaatccgtg
     5581 atgatgtcct gcaatgccta ttattgcaac gtcctgagaa gcatccttga taatcctgct
     5641 tacggaagta ttgcagaagc catggacaaa tggcaggcat acgttaaaag ttttggtttt
     5701 ggcacccgcc tgggtatcga tatgcccggt gaattggccg gttcgcttcc gggttcagcc
     5761 ctttacgacc ggatttatgg caaaaaccgg tggcggtctc tcaccgtaat ttcgctttcc
     5821 ataggtcagg gagagattgg cgcaacaccg ctccagctgg ccaacctggc cgcaatcatg
     5881 gccaacaggg gacactacta taccccgcac ctggtaaggg aaattcctga agatccctca
     5941 ttcaggccca acgtggagcg gcaccaggtg atggtagatc ccgaaaattt ccggattatc
     6001 gtagagggaa tgtaccaggc tgtaaacgcc cctgcaggtt cgggcgccac ggcccgccag
     6061 gctgccatcc cgggggtgga aatgtgcgga aagaccggaa cggcacagaa tccccatggg
     6121 gaagaccatt ccgttttcat ctgctttgcg cctaaggata atcccaggat tgccgttgcc
     6181 gtatacattg aaaaaggcgg attcggagcc tcccaggcag ctcccgtggc ttcactc
//
LOCUS       DAOSOF010000113         6139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12902_length_6139_cov_4.446252, whole genome shotgun sequence.
ACCESSION   DAOSOF010000113 DAOSOF010000000
VERSION     DAOSOF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12902_length_6139_cov_4.446252"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLM68_06795"
     CDS             <1..406
                     /locus_tag="PLM68_06795"
                     /inference="COORDINATES: protein motif:HMM:NF039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HOT55102.1"
                     /translation="DTTHTWISGLWNDTEGTVRGLTKETAYTIAEARNLPGGLSDIWV
                     CGYIVGCYSSGGTYFYSGNESATNLALADTNSEMDKANTYPVELPAGAIRDALNLVDN
                     PQHLSKKIWIRGNTSASYFSLPGLKSAKDYSW"
     gene            complement(539..1558)
                     /locus_tag="PLM68_06800"
     CDS             complement(539..1558)
                     /locus_tag="PLM68_06800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT55103.1"
                     /translation="MKKLIYLTMAMLATIPALLSSCAREQTESTLEAHKKMLAAHVRI
                     IHQDTLQKTESGVYYTIVRKGSGAPSTDSSIVFVRETVLDLKYNIIASTEENVARQLG
                     NFSHANAYIPLLWYMGNNSIMMGLEEMLQDMKEGEMRRIWLPYWLSAYQEGGSSENTT
                     AMVYDLELVKVVSDIDKYQIDTLESFRNRHYPGVDSLERGFYKVTLVPGTGDSVKVAT
                     TAKAWYIGKFLNGHVFDTNVADTAQKYRIYDSDNEYSVLQVSMPSEEEEEEDTSEEEG
                     SVVKGFSKCMQEMRYGEVAVCFFHSDYGYKLEGKQSSASGTYLGGGIPSYMPLFFWIY
                     VPLDD"
     gene            complement(1555..2106)
                     /locus_tag="PLM68_06805"
     CDS             complement(1555..2106)
                     /locus_tag="PLM68_06805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT55104.1"
                     /translation="MRKYVFLLAGLLLAITSCTKEDRKLMLIRQEEEIDKFVRNLVKD
                     TVYYQKGVVRAVLEPGKHAGPADTLATGDTIYFYYAGHVFSRGKGALFHTNSDSVAKA
                     YNRTLPEDQSAIRSGVTGEGKFLKGLEYGFTGMSAGEHAYLIFNADFGYGNETVGQVP
                     RMSPLLFEIWVERIVKKQISLQE"
     gene            complement(2120..3130)
                     /locus_tag="PLM68_06810"
     CDS             complement(2120..3130)
                     /locus_tag="PLM68_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT55105.1"
                     /translation="MKVLFPKNLVAKWDQALAQSSHIVVATHINPDGDAVGAMIGLGS
                     FLRQQGYRVTCICPSPYPGFLAFLDKEKQVLVYSHQKEEVIRAVDKADMIVAVDVSGL
                     SRMEEMGEFLLRIRAIKVLMDHHIDPVVEDFDLVFSTPQVSSTCELIFRVLNSWKPDA
                     VFTRDQATAFLTGIITDTNQFANSVFTETFHVASLLHGWGADSETIHSAVFQHFTLNR
                     MRLMGHALSNIKMLPQFGAAYIVLTRDVLESCDYRNGDTEGFVNIPLAIKGISISALF
                     LQREDHIKVSLRSKGAIAVNGIAKQFFQGGGHYNASAGKMEDDPGKIEELLKEALGTI
                     IA"
     gene            3227..3829
                     /locus_tag="PLM68_06815"
     CDS             3227..3829
                     /locus_tag="PLM68_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55106.1"
                     /translation="MKKLLTIVILPLIIIGLGYAIVRSLTAPIKFDKERDFRSSLAIE
                     RLKDIRTLQTTYKTEFGKYASTFDTLENFYKNGTITIVRQIGSFDDSVAVAQGRVFRD
                     SMELPVKDTLLKYPGFNIDSLRWVPLVGEEFEMRAVVKKVSGVDVPLFEACTPFDVLL
                     KGMNRQLIVNLNEDLKISNRYPGIKVGSIDQPNNNAGNWE"
     gene            3834..4652
                     /locus_tag="PLM68_06820"
     CDS             3834..4652
                     /locus_tag="PLM68_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOT55107.1"
                     /translation="MSAISKIPQAHHRLSIHIGVNGLSFIIQDTNTNECLLWQHLSYS
                     FPRNDYNEWAKEVGLVFRQEEYLQKRFNQCYVMIDTHKHTLIPTSVFQPEQAIDVLRQ
                     LFDVAYLDEVNYSELVTQEATCIYALPAPVNAMVFKYQHKARFFASVMPLLYFIMEQK
                     EYSRALMHYSHSHVDLVLMQGERLLLCNSYAIDRFSTGLYYLLFALKQWQLNPGSLNL
                     YISGPFKPTHTKQLCTYFPSVTVATSPSFLFPSEALNLLYGPHLFPVCASSVEV"
     gene            4628..5224
                     /locus_tag="PLM68_06825"
     CDS             4628..5224
                     /locus_tag="PLM68_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOT55108.1"
                     /translation="MRIISGSLRGHNILPPKSFKARPTTDFAKEGLFNVLDNSMDLTA
                     IAALDLFAGSGSISLEFASRGCPSVTCVEMNPDYARFIKDTVSALHLSSQVQVLRNNV
                     FDFLNICTRKYNLVFADPPYDLPGLKNLPEKIFNAGILEDDGMVVLEHPDAFSFAGSA
                     HFMKEKKYGNVHFSFFAVSKTLPTFALPNNQGPVVQPG"
     gene            5201..5275
                     /locus_tag="PLM68_06830"
     tRNA            5201..5275
                     /locus_tag="PLM68_06830"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5236..5238,aa:Asp,seq:gtc)
     gene            complement(5436..5639)
                     /locus_tag="PLM68_06835"
     CDS             complement(5436..5639)
                     /locus_tag="PLM68_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55109.1"
                     /translation="MKERKLSREARAILLTGLARGYFTYDELSRLPDMIGLERYQPRV
                     DDMSKEEIMEEIEKIKDRMRQCQ"
     gene            complement(5636..5965)
                     /locus_tag="PLM68_06840"
     CDS             complement(5636..5965)
                     /locus_tag="PLM68_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55110.1"
                     /translation="MEKDIREKLLEAAEKGAINKMEFAELTGQKIIEIEQCLAQECVN
                     CTRKGAPIHKDMNTQMAINTDLKKDIIKAIHADDLSVLTNGTPLVLYVGEWENCKKCN
                     ILKNQKP"
BASE COUNT         1582 a         1519 c         1293 g         1745 t
ORIGIN      
        1 agacaccacg catacctgga taagcgggct ctggaacgat actgagggaa cggtgcgcgg
       61 ccttaccaaa gaaacggcct ataccattgc cgaggcccgt aacctgcccg gcggtctgag
      121 cgatatctgg gtatgcgggt atattgtggg ttgttattct tccggcggga cgtactttta
      181 ctcaggtaac gaaagcgcga caaacctggc ccttgccgac accaactctg aaatggacaa
      241 ggcaaatacg tatcccgtag aactgccggc gggagccata cgtgatgccc tgaacctggt
      301 ggataatccg caacacctgt caaagaaaat ctggataaga ggaaacacca gtgccagtta
      361 tttttcattg ccgggactga aaagcgcaaa ggattattcc tggtgataaa acaacaagag
      421 gtggtccgag aaccggaccc acctctaccg ttgaaaccta taaacctaaa accacataat
      481 agatgcaggt gcatgcaaaa acgttgcatg cacttgtacc aattttttta ttatgcggtt
      541 aatcgtctaa tggtacgtat atccagaaaa acaaaggcat ataggaaggg atgccaccgc
      601 ccaggtaagt gcccgaagcg gaggattgct tgccttccag cttatagccg taatctgaat
      661 ggaaaaaaca tacggccacc tctccgtacc tcatttcctg catgcatttg ctgaacccct
      721 tgaccactga tccctcctcc tcgctggtgt cttcctcctc ctcttcctcg gatggcatag
      781 atacctgaag gactgaatat tcattatcgg agtcgtaaat cctgtatttt tgggctgtat
      841 cggccacatt ggtgtcaaat acatgaccat taagaaactt acctatatac caggctttcg
      901 ctgtagttgc aactttcact gaatccccgg taccagggac cagggtcact ttgtaaaatc
      961 ctctttccag ggaatcgact ccgggatagt gcctgttccg gaaagactcc agggtatcta
     1021 tctggtactt gtcaatatcg ctgaccactt tgaccaattc caaatcatat accatggctg
     1081 tggtattttc tgaacttccc ccttcctgat aagccgataa ccaataagga agccaaatcc
     1141 ggcgcatttc accttccttc atgtcctgga gcatttcttc cagtcccatc atgatggagt
     1201 tgttccccat ataccataat agggggatgt aggcattggc atggctgaaa ttccctaatt
     1261 gcctggccac attttcttct gtgctggcaa tgatgttgta cttcagatca agaacggttt
     1321 cccttacaaa tacaatggat gagtctgttg acggggcacc tgaacctttg cggactatgg
     1381 tgtaatatac acccgattcg gttttttgca atgtatcctg gtgtatgatc ctgacatggg
     1441 ctgcaagcat ttttttatga gcctcgagcg tagattcagt ctgttccctc gcacaggaag
     1501 acaacaatgc cggaatggtt gccagcatgg ccattgtcag gtaaatcaat tttttcattc
     1561 ttgcaaagat atctgttttt tgacaatccg ctccacccat atctcaaaca ataagggaga
     1621 catccggggc acctgtccta ccgtttcatt cccgtatcca aaatcggcat tgaaaatcaa
     1681 ataagcatgt tcgccggcag acatgcctgt aaaaccatat tccagtccct tcaggaattt
     1741 cccctctccc gtaaccccgg aacggatggc agactggtct tcaggaagtg tcctgttata
     1801 agctttggcc acactgtccg aattcgtatg gaataaggca cctttacccc tggaaaaaac
     1861 atgtccggca taatagaaat aaatggtatc cccggttgcc agtgtatcag ccgggccagc
     1921 atgttttccc ggttcaagaa cagcccttac cacgcccttc tgataatata ccgtgtcttt
     1981 gacaaggttc cggacaaact tgtcaatctc ctcctcctgc ctgatgagca tcagcttgcg
     2041 gtcttccttt gtacaggaag tgatagcaag gagtaatccg gctagtaaaa atacatactt
     2101 tctcatgtat gacctgcaat catgcaatta ttgtgcccag ggcttctttc agcaactctt
     2161 caatcttgcc gggatcgtct tccattttac ccgccgaggc attgtaatgt cctcctccct
     2221 ggaagaactg ttttgcaata ccgtttacgg caatggctcc ttttgacctg agtgacactt
     2281 ttatgtgatc ttccctctgg aggaataagg ccgagatgga gatccccttg atggcaagcg
     2341 gaatattgac aaaaccttcc gtatcgccgt tgcggtaatc acaggattcc aggacatccc
     2401 ttgtcagaac tatatacgct gctccgaatt gcggcaacat cttaatattg ctcagtgcgt
     2461 gccccatgag ccgcatccgg ttcaatgtga aatgctggaa cactgcactg tgtatggttt
     2521 cagagtccgc accccaaccg tgcaggagtg aggcaacatg gaatgtttcc gtaaagacgc
     2581 tgttggcaaa ttgattcgta tcagtaataa taccggtcaa aaaagcagta gcctggtccc
     2641 tggtaaatac agcatctggt ttccaggaat tgagcactct gaatatcaac tcacaagtgg
     2701 agctgacctg tggtgttgaa aagaccagat caaaatcctc cacaacggga tctatatgat
     2761 gatccatcaa gactttgatt gcccgtatac gcaataagaa ctctcccatc tcttccatgc
     2821 gtgataatcc gctgacatcc accgccacta tcatatctgc cttgtccacc gcccttatga
     2881 cctcttcttt ctggtgtgaa taaaccagaa cttgtttttc cttgtctaaa aatgccagga
     2941 aaccgggata aggactcggg cagatacatg tcacccggta accttgttgt cttagaaaag
     3001 atccgagtcc aatcatggcc cctacggcat ccccgtccgg attgatatgg gtagccacca
     3061 caatgtgtga cgattgggct aaagcttgat cccatttggc taccagattt tttggaaaca
     3121 ggactttcat aaaaagatat tgttgcaaat ataattgatt tattgcagag gttatttttt
     3181 ttcctaattt tgcaggacgt ctgttagatt atttaataaa tcaataatga aaaaattact
     3241 caccatcgtt attctgccct tgattatcat tggattgggc tatgctattg tcaggagcct
     3301 cactgcgccc ataaaattcg ataaagaacg tgatttccgc agttcgttgg ccattgaacg
     3361 tttaaaggac atccgcacac tgcagaccac ctacaaaaca gaatttggaa agtatgcatc
     3421 tacttttgat accctggaaa atttctacaa aaatggtact ataacgattg taaggcagat
     3481 aggttcattt gacgactcag ttgctgttgc acagggtcgc gtatttcgtg acagcatgga
     3541 attgcctgtt aaggacacat tgctcaaata tccgggtttc aacatagatt ccctgcgctg
     3601 ggtcccgctg gtcggagaag agtttgaaat gagggccgtg gtaaagaagg tatcaggtgt
     3661 tgatgttccg ctctttgagg catgtacacc ttttgatgtt cttttgaagg gaatgaaccg
     3721 tcagctgata gtcaatctca atgaggatct gaaaattagc aaccgttatc ccggtatcaa
     3781 ggtggggagt atagaccaac caaacaacaa cgcgggtaac tgggaatagc aacatgtctg
     3841 cgatcagcaa aataccgcag gctcatcaca gattgtccat tcatatcggg gtgaatggac
     3901 tttcttttat tatccaggac actaatacaa acgagtgcct tttatggcag catctttcat
     3961 attcgttccc acgcaacgat tacaacgaat gggcaaaaga agtggggctc gtcttcaggc
     4021 aggaagaata cctgcagaaa cgcttcaacc agtgttatgt catgatagac acacacaagc
     4081 atacactcat ccccacctct gtttttcagc ccgaacaggc aattgatgta ctccgccagc
     4141 tattcgatgt ggcgtacctg gatgaagtca actattctga actggtcaca caggaagcca
     4201 cctgcattta tgccctcccg gcccctgtca atgccatggt ctttaagtac cagcacaagg
     4261 ccaggttttt tgcctcggtc atgcccctgc tctatttcat tatggagcaa aaagaatact
     4321 caagagcttt aatgcactat tcccactccc atgtggacct ggttttgatg cagggggaac
     4381 ggctgttgct ttgcaattcc tatgctattg accgtttcag cacaggtctc tattatcttt
     4441 tgtttgccct gaaacaatgg caactaaacc ccggaagcct taacctgtac atcagcggac
     4501 ctttcaaacc cacgcacaca aagcagctgt gcacttattt cccctccgtg acggtagcca
     4561 cctccccttc tttcctcttt ccgtcagaag ccctgaacct gctgtacgga cctcatcttt
     4621 tcccggtatg cgcatcatca gtggaagtct gagagggcat aacatactgc cgccaaaatc
     4681 cttcaaagca agacccacca ccgattttgc gaaggaagga cttttcaacg tgttggataa
     4741 ttccatggat ctgaccgcca ttgccgcgct ggatctgttt gccggaagcg gcagcatatc
     4801 actggaattt gcttcaaggg gatgtccttc cgttacctgt gttgaaatga atccggacta
     4861 tgcccgcttt attaaagata cggtgtcagc attgcacctg tcatctcagg tacaggtatt
     4921 gcgaaacaat gtgtttgatt tccttaatat ctgtacccgt aaatacaacc ttgtttttgc
     4981 cgatcccccc tatgatctgc caggcctgaa aaacctgccg gaaaagatat tcaatgcggg
     5041 tatcctggaa gatgacggaa tggttgtatt agagcatcct gacgcatttt ccttcgccgg
     5101 gtcagcgcat tttatgaaag aaaaaaaata cgggaacgtg catttcagtt tttttgcagt
     5161 ttcaaaaaca ttgcctacat ttgccctccc aaacaatcag ggtccggtag ttcagcctgg
     5221 ttagaatacg tgcctgtcac gcacggggtc gcgggttcga gtcccgtccg gaccgctcca
     5281 cacatctcca gcaaattgtt aatcagtttg ttggattttt tttaaaaata aattcaccga
     5341 ctttttcacc gacttaataa aaaaaggcgc ccatttgagc gcccattttc aatgacattt
     5401 ttatgtgtga tgttgataca aagatacaaa aaaatttatt gacattgtct catccgatcc
     5461 tttatttttt cgatctcctc catgatctcc tctttggaca tatcatcaac ccgtggctgg
     5521 tatctctcca ggccgatcat gtcgggtagc cgggaaagct catcgtatgt gaaatatccc
     5581 ctggctaaac cagttaataa aatggccctg gcctctctac ttaattttct ctctttcatg
     5641 gtttttggtt ttttagtatg ttacattttt tgcagttttc ccactcacca acatacaaca
     5701 ccagcggcgt tccgtttgtc aaaacagaca agtcatcggc atgtatggcc tttatgatgt
     5761 ctttttttag gtcggtgtta attgccattt gagtattcat gtccttgtga atcggcgccc
     5821 ctttccgtgt gcagttcacg cactcctggg caaggcattg ctcaatttca atgattttct
     5881 gccccgtcag ttcggcaaat tccatcttat tgatagctcc tttttcggct gcttccaaca
     5941 atttttctct tatatctttt tccatcttat tgattattta tgtgtttctg tacttttcat
     6001 tttggcgatt atcaggtcca ccgcctcatc gggtgctttt tccaatagtt cggctaatga
     6061 tctcatctct gcctcgatca tagcctcaac ggacaccgac tgcattttag ggatgctata
     6121 ctgcaaaagt ttttcaaca
//
LOCUS       DAOSOF010000114         6121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_12965_length_6121_cov_4.994230, whole genome shotgun sequence.
ACCESSION   DAOSOF010000114 DAOSOF010000000
VERSION     DAOSOF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12965_length_6121_cov_4.994230"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PLM68_06845"
     CDS             <1..1078
                     /locus_tag="PLM68_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT55111.1"
                     /translation="RKAPTLAYLYPDKAYFDLLNFDNSTSSYGTADQHFQVITTRIFD
                     TENEELKMAKTQKIELGLSGKIGQVTYSVVAYKEAGENGYTFSKGINTFKSVPLTIYP
                     ALKQADYPADGSLPALKVPTTYQYLISYSQPSNNGVHHQKGIEFDINFGRIDAIRTSF
                     ILNGAWYESEDYRNGYSFWNKNKSTSYDQYPHMGVFDGDVNGTTHYRNFSTNLIITHN
                     IPELGFVVSLTANVVWGEKAWQTYGNDTIPVAYISRQDGKLYDFDPAWFDDPAKSAEF
                     AGIDRRNDVSDMRMLIEPTLPPLICFNFNVTKQFGDFLDVSFYAQNMFRSTPLYESQI
                     HPGSYKRRNANVFFFGLQLTAKIK"
     gene            1093..2376
                     /locus_tag="PLM68_06850"
     CDS             1093..2376
                     /locus_tag="PLM68_06850"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HOT55112.1"
                     /translation="MKKIFLLSAVVCLFSCLATSCIKMMQEANEAEKVHMITVSYKVN
                     AVTGFVPYSGEAEPDPSFQTKGLEVNFINYQEGSSFKSVVGDNNIVTCSLTPGVYAVN
                     VSGSVTNPKGTYRLNGSSLNVPFTTDITPQEATSNPQYTINIRPAKVGSVMFSEIYYC
                     GVAPYYFRDQTYTIYNNGDEVFYLDGLCFAQLHPNIATTNLPSWPEEDGRENFVYGLV
                     IWQFPGTGKQYPLQPGESVVIAQEAINHKVNNPASYDNSMSEWECWSGNALRGNPDCP
                     DMPYIFWWSPNSMQWLTSVFGAAFCLFRIDDAVVNKAYWEQEGHWQRVIGPSSTRYAK
                     IPADAVLDGVELLTSMSALNMKRIPGFVDAGGTSVETTYIGKSVSRKVIGQRANGSPI
                     YQDTNNSTNDFQVNDSPAIRRDGQKIPAWSRANDL"
     gene            2396..3988
                     /locus_tag="PLM68_06855"
     CDS             2396..3988
                     /locus_tag="PLM68_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55113.1"
                     /translation="MRKNMIKWIGCLALLTSSYFAAKAQTPVTPASVDRLKMESLWLR
                     HTNNVAGAILDNPWHYALTDFEYQIEKGEFKRVQSGNNNNAFRFNTEGGGIYEALGGA
                     YAWGEFHYDRSVLRGAKYNASLSDPLRGMPYIIADPNPSKWINQNYSMQVKLASPLIW
                     NQLVLGVGATYENGIAAKQVDPRPKVLLSELSVNPALVWTKGRHAVGADFVYLSHRED
                     GAASNSNYRVPQMGWEMLFPGYFMEGEISSFGSITGLRNFNANALGGGLQYGYEGPKF
                     KVVAAGKYRQTTENVTNSYTTPKRIGTTNKKEWTATAGAQFLFNDRNSLFADYKYYNR
                     SIDGIEYIQIYNPAYESQEWIVYGKYIKSNYSTVSHNFGLDYMISKDGRSDFSWKFGV
                     NAQAEKLSDVYYLPESTQEVNNFLVKAYVKKNFVTGKRSSLMLMLEGFLNKNTNAEHN
                     YGGYYENNVAYTQFALMDFYYLSSSYNGFTLDASYTISNIDRKQRTGVYVGASFNYVN
                     PTSYKDYFDKRSTLTVKVGLAF"
     gene            complement(4189..5862)
                     /locus_tag="PLM68_06860"
     CDS             complement(4189..5862)
                     /locus_tag="PLM68_06860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT55114.1"
                     /translation="MKQSILLFTALLALTANRAHACTNLLITKGASADGSCMVTYAAD
                     SHTRYGCLVFYPAALHPAGTMMDVREWGTNRYLGKIPQARETYSVVGNMNEHQVVVGE
                     STWGGLSSHHDPQGIMDYGSLMYIALQRARTARQAIEIFTTLANEYGYASSGESISFA
                     DPHEVWFMDVIGKAPRMVNGENVNKGIVWVAVRLPDGTISAHANQARIRRFPLDDPQN
                     CLYAPDVIEHARETGLYSGPDSLFSFAEAYGPADGGTIRGCDARVWAFFNKHGAEDMD
                     PYLPYALGHDPDNPLPLYVKAKEKLTVKQVADMMRDHYEGTPMDMTCDIGAGGHDLPY
                     RWRPMGFEVDGKQYLNERAIATQQTGFWFVGQCRSQWPHEIGGIFWFGVDDAGTSPLT
                     PVYTSSLEVSPHYALGQGASMLQYSPLSMFWLCNRIAQFAYLRYNQIGAEVRQAVDDH
                     ENARLAEIPLTDKQALELWERDPLEARRFLTDYSLRTASNLFRRWQELDVYLLVKYID
                     GNIKRQNPDGSFATNGHSDSIPPAPIYGGYNQRWKEAVVKDTGERLLAP"
     gene            complement(5877..>6121)
                     /locus_tag="PLM68_06865"
     CDS             complement(5877..>6121)
                     /locus_tag="PLM68_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55115.1"
                     /translation="WYQGIELFTALRRLGKPVWMLQYNSESHNLGERVNAKDLSIRLE
                     QFFNHFLKGAPMPVWMKTGVPATHKGIDWGLELTTP"
BASE COUNT         1556 a         1591 c         1576 g         1398 t
ORIGIN      
        1 ccgcaaagca cccaccctgg cataccttta tccggacaaa gcatactttg acctgctgaa
       61 ctttgacaac tccacatctt cctacgggac agcagaccag cacttccagg tcattaccac
      121 acgtatcttc gatacagaga acgaagaact gaaaatggcc aaaacacaaa agatcgaact
      181 gggcctgagc gggaagatag gacaggtgac ctattccgta gtcgcataca aggaagccgg
      241 tgaaaacggg tataccttca gtaaaggtat caatactttc aagagcgttc ccctgaccat
      301 atatccggcc ctgaaacaag ccgattatcc ggctgacggg tcgttgccgg cacttaaggt
      361 ccccacaacc taccaatacc tgataagcta ttcccagccc tcaaataacg gcgtgcacca
      421 tcagaagggg attgaattcg acatcaactt cggccgtatc gacgctatcc gcacctcgtt
      481 catcctgaac ggcgcctggt atgaatcgga agattaccgc aatggatatt ccttctggaa
      541 caagaacaaa tccacctctt atgaccaata cccgcacatg ggggtatttg atggtgatgt
      601 gaacggtacc acgcattaca ggaacttctc caccaacctg atcattaccc acaacatccc
      661 ggaactgggg ttcgtcgttt ctttgaccgc caatgtggta tggggtgaaa aagcctggca
      721 gacatatggg aacgatacca ttcccgtggc ttacatatct cgtcaggatg gcaaactata
      781 tgatttcgat cctgcatggt ttgacgatcc tgcaaagtcc gctgaatttg ccgggataga
      841 tcgcaggaat gatgtgagcg acatgcgcat gctgatagag cctacactgc caccgctcat
      901 ctgcttcaac tttaacgtaa ccaaacagtt tggagacttt ttggatgttt cgttctatgc
      961 acagaatatg tttcgcagca caccgttgta tgaaagccag attcatcccg gttcatacaa
     1021 gagaaggaat gcaaacgtct tcttcttcgg attgcagtta acggcaaaaa ttaaataaag
     1081 ggagactacg atatgaaaaa gatattttta ttaagcgcgg tcgtatgcct tttctcttgt
     1141 ctggccactt cctgtatcaa aatgatgcag gaagccaatg aagccgagaa agtccatatg
     1201 ataacggttt cgtataaagt aaatgccgtg acgggttttg tcccttacag cggagaagcg
     1261 gaacccgatc cgtctttcca gaccaaaggc ctggaagtga atttcatcaa ttaccaggaa
     1321 gggtcgtcct tcaagagtgt ggtgggggac aacaatattg tcacctgctc cctcactccc
     1381 ggtgtatacg cagtaaatgt ttcgggaagt gtcaccaatc ccaagggtac gtaccggctg
     1441 aacggttctt cactgaatgt tccattcacc acagacatta ccccacagga agcaacttcc
     1501 aatccccaat ataccattaa catccgtccg gcaaaagtgg gatcggtcat gttcagcgaa
     1561 atatactatt gcggggtggc tccgtattat ttccgggacc aaacctacac catctacaat
     1621 aatggagatg aagtcttcta ccttgacggt ctctgctttg cacagcttca cccgaacatt
     1681 gccaccacca acctgccctc gtggcccgaa gaagacggtc gtgaaaattt cgtatacgga
     1741 cttgttatct ggcaattccc cggcaccggg aagcaatacc ccctccagcc gggagaatcg
     1801 gtggtcatag cccaggaggc tatcaaccac aaagtcaata atccggcttc gtatgacaat
     1861 tccatgagcg aatgggaatg ctggtcaggg aatgcccttc gtgggaatcc cgactgtccc
     1921 gatatgccct atatcttctg gtggagtccc aacagcatgc agtggctgac ctcggtattc
     1981 ggtgcagctt tctgcctctt caggatagac gacgcagtag tgaataaagc ttactgggaa
     2041 caggaaggac actggcaaag ggtgataggc ccctcttcaa caaggtatgc caagatccct
     2101 gcagacgcag ttctggacgg ggttgaactg cttacctcta tgagtgcgct caatatgaaa
     2161 cgcatacccg gctttgtaga tgccggaggc acttctgtgg aaaccaccta tatcggcaaa
     2221 tcggtttcac gtaaggttat tggccaacgc gccaacggtt cgcctatcta tcaggacacc
     2281 aacaattcta ccaacgactt ccaggtgaac gactcgccgg ccatccgtcg cgacggccag
     2341 aagatccccg cctggagccg tgccaatgat ctataacctt taaatgatta ctgctatgag
     2401 aaagaatatg atcaaatgga taggatgcct ggcactcctg acaagtagtt attttgcagc
     2461 caaggctcag accccggtca ctccggcttc tgtggatcgt cttaaaatgg aaagcctttg
     2521 gctcagacat accaacaatg tggccggggc gatcctggat aatccctggc actatgctct
     2581 caccgatttt gaataccaga tagaaaaagg ggaattcaaa cgggtacaaa gcgggaacaa
     2641 caacaatgcg ttccggttca acacagaagg tggcggaatt tatgaagcac tgggcggtgc
     2701 ctatgcctgg ggcgaattcc attacgaccg gtctgtgttg cgtggcgcca aatacaatgc
     2761 ttcgctgtcc gatccgttgc gcggcatgcc ctatatcatt gctgatccaa atcccagcaa
     2821 atggatcaac cagaattatt ccatgcaggt gaagctggcc tcgcccctta tatggaacca
     2881 gctggtcctg ggagtcggag ctacctatga aaacggcatt gccgccaagc aggttgaccc
     2941 acgtcccaaa gtgctgttat cggaactgag tgtgaatccc gccctggtct ggactaaagg
     3001 caggcacgct gtaggagctg actttgttta tctctcgcac cgtgaagacg gggctgcttc
     3061 aaactccaac taccgtgtac cccagatggg ttgggaaatg ctgttccccg gctatttcat
     3121 ggaaggggag atcagcagct tcggaagtat taccggtttg cgcaatttca acgccaacgc
     3181 actgggcggc gggttgcaat acgggtatga aggcccgaag ttcaaagtgg tagccgctgg
     3241 aaaataccgt cagacaacag aaaatgtcac caattcctat acgacgccca aacgcatcgg
     3301 gaccaccaac aagaaagagt ggacagccac cgcaggggca caattcctct ttaacgacag
     3361 gaattcgtta tttgcagatt ataagtatta taaccgcagc attgacggga ttgaatacat
     3421 ccagatttac aatccggcct atgaatccca ggaatggatc gtatacggga agtacatcaa
     3481 gtcgaactat tcaacagtga gccacaattt cggcctggat tacatgatct cgaaagacgg
     3541 acgcagtgat ttctcctgga agttcggagt caatgcacag gcagaaaagt tatcagacgt
     3601 ttactacctg ccggaaagca cgcaggaagt gaataatttc ctggttaagg cgtacgtgaa
     3661 aaagaacttt gttaccggca aacgcagcag cctgatgctt atgctggaag ggttcctgaa
     3721 caagaacacc aatgcggaac acaattatgg cggatactat gaaaacaacg tggcatacac
     3781 gcagttcgcc cttatggatt tctactacct gagcagttcc tacaacggtt tcacgctgga
     3841 tgccagctac acgatatcca acattgaccg taaacaaaga acgggcgtgt acgttggtgc
     3901 atcattcaac tatgtgaatc cgacgtccta caaggattat ttcgacaaac ggagtaccct
     3961 gacagtgaag gtaggtctgg cgttctaaac ggaatgccca tatttaagag actgaccatg
     4021 attaatgggt cagtctcttt ttttgtattc tggcagccgc agaagtgggt gccccgcaga
     4081 ccagattctt ttgactcata atcagctatt tatgcaactc atacccccgg gggtatgaaa
     4141 cgcataatgc tttgaaagcc agctaaaaga acaatgtgtg caacgcacct atggggccag
     4201 cagccgttcc ccggtgtctt tgacaacggc ttctttccag cgctggttgt agccgccgta
     4261 aatgggagcg ggagggatag agtcggagtg gccgttggtg gcaaagctgc cgtcggggtt
     4321 ctggcgcttg atgttgccat ctatgtattt aaccaacagg taaacatcca gttcttgcca
     4381 gcgcctgaac aggttcgatg ccgtgcgcag cgagtaatct gtcagaaagc ggcgggcttc
     4441 caaggggtcg cgttcccaca actccaacgc ctgcttatcg gtcagaggga tttcggcaag
     4501 gcgggcattc tcgtgatcat ccacggcctg ccggacttct gccccgatct ggttgtagcg
     4561 caggtaggca aactgggcaa tgcggttgca cagccagaac atggacaggg gcgagtattg
     4621 cagcatggag gctccctgcc ccagcgcata atgcggagac acctccaacg aggaagtata
     4681 aacgggagtc aggggcgaag ttccggcatc gtccacacca aaccagaaga tgcctcctat
     4741 ttcatggggc cactgggagc ggcattgtcc aacgaaccag aaaccggttt gctgggtggc
     4801 tatagccctt tcgttgaggt attgtttccc gtccacttca aagcccatcg ggcgccagcg
     4861 atagggaagg tcgtgacctc cagcccctat gtcgcaggtc atgtccatgg gggtgccttc
     4921 gtaatggtcc cgcatcatgt cggccacctg tttgacagta agtttctctt tggctttcac
     4981 atacaacgga aggggattgt cagggtcgtg tcccagcgcg taaggaaggt aaggatccat
     5041 atcctcggcc ccgtgtttgt tgaaaaaggc ccacacccgt gcgtcgcagc cacggatggt
     5101 gccaccgtcc gccggaccat atgcctcggc aaagctgaaa agactgtcgg ggccgctgta
     5161 aaggccggtt tcccgtgcat gttcgatcac gtctggagca tacaggcaat tctgagggtc
     5221 atccagggga aagcgacgga tcctggcctg gttggcatgt gccgatatgg tcccgtcggg
     5281 caggcggacg gccacccata caatcccttt gttgacgttc tctccgttca ccatccgggg
     5341 cgctttgcca atcacgtcca tgaaccagac ctcgtgcgga tcggcaaagg atatggactc
     5401 tccggaagag gcgtagccgt actcgttggc cagggtggta aagatctcaa tggcctgccg
     5461 agcggtccgg gcacgctgca gggcaatgta catgagcgag ccgtaatcca tgatcccctg
     5521 ggggtcatgg tgcgaggaca atccgcccca ggtactttca cccacaacca cctggtgttc
     5581 attcatgttt cccaccaccg aatacgtttc cctggcctgt gggatcttgc cgaggtaacg
     5641 gttggtgccc cattcgcgga catccatcat ggtgccggca ggatgcaggg ccgccggata
     5701 aaacaccagg cagccatacc gggtatgcga atcggccgcg taggtgacca tgcaggaacc
     5761 gtcggcggag gctcctttgg tgatgagtaa attggtgcag gcatgggcgc ggtttgctgt
     5821 gagcgccagt agtgcagtaa aaagcaagat gctctgtttc atgatgcaag atttctctac
     5881 ggggtagtca gttccagtcc ccagtctatg cctttgtgag tggcgggaac gcctgtcttc
     5941 atccaaacgg gcatgggagc tcctttcagg aaatgattga agaactgttc caggcgtatg
     6001 ctaaggtcct ttgcattcac acgttcgcca aggttgtggg attcgctgtt gtactggagc
     6061 atccacacgg gtttgcccaa cctgcgcaag gccgtgaaga gttcaatgcc ctggtaccac
     6121 g
//
LOCUS       DAOSOF010000115         6083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_13084_length_6083_cov_4.560717, whole genome shotgun sequence.
ACCESSION   DAOSOF010000115 DAOSOF010000000
VERSION     DAOSOF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13084_length_6083_cov_4.560717"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLM68_06870"
     CDS             <1..803
                     /locus_tag="PLM68_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55116.1"
                     /translation="EGVFAFMGFTVIIPDLIGSGISAHVPQPFLFMDNTGRVCYDMQL
                     AAMEFFSAYGAAPLSPEIDIFGYSGGATGAVALLRHIDGLPDSPVRVKRLIAGAGVYD
                     MRETYKVLKERGFAEYPLVPLMIRSLDYWYDLQLDYSLVFTGDLLANREEWLDGTRNA
                     SQMRELISPQLNTYMHPDFFSPESNSEIKKIENALALHSMKEGWNTRTRIYLGHATDD
                     MSVPFSETQAFFNSMNSKSKTSMTKGAGGHYHYGIEFFISGCLFLTAN"
     gene            806..1945
                     /locus_tag="PLM68_06875"
     CDS             806..1945
                     /locus_tag="PLM68_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT55117.1"
                     /translation="MSRYFKEVLKEGDFSITDVFTEVKKDFPALDLSRIEPLIRTLFK
                     MRVRITAISYNTVDPLENPVLSSGIIMRPINRKPRGVLHFLPSANIDKFGSGSDALIL
                     FEGVLSFLGYIVIIPDLLGNGITKDTVEYPFLMAENTGQVAYDMHRAAIEYYQEVLGH
                     PLPKHISIGGYSMGGSGALALVRHIERENPSDIIVDRAIIGGGLYDLNVAFEEFAKTG
                     FAQYPAAPGVFKAYDKWYNLNLDYSRVFTGPLLENMDSWLDRTHYRDQLTEWIGQDLH
                     KYMHPDFFTPERNSEIKKLWDKFRENSLADGWTPKTHILIAHASDDLSVPTRVATYTV
                     EQFRKRGARIRSRYGKGGHYEFGKWFFLRMGIHLILKRMAFWTRF"
     gene            2030..3367
                     /locus_tag="PLM68_06880"
     CDS             2030..3367
                     /locus_tag="PLM68_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT55118.1"
                     /translation="MKKSACILVLIFSFAQGQLLRAQDTLYVNLETALEIALSESPVI
                     RIADREVERAFYARKETQSGLWPKISASGMYNRTLKKQVMVMDFGGQEMEISVGTDNN
                     WMGGVSLSLPLVAPALWKTVQLSALDIDLAVEKARSGRLAMISAVKQAYYSLLLAKDS
                     YVVLKKNYDNVALNTKTVTDKYEQGMASEFDKLRAEVALKNQHPNLLASETAIELASM
                     QLKALMGLDIEYPVVFEGSLIQYEGEVTPGGLSAASEFSLENNTDLMQLAIQKQQLEL
                     AMKITKASFLPTLSLSSNYQYSSMNNDFRFSNYNWYPYSTASLALNIPLFSGMKRQQQ
                     VRQSKLTLQTLEDSRINSERTLRLNVNNCLNQMGNAVEEFSSNKENVAMAEKAYSISR
                     KQFEVGMGTWLDLSASELALTQARLAYHQSVYSYLFYRAELEKITGTSNYQNQ"
     gene            3371..4387
                     /locus_tag="PLM68_06885"
     CDS             3371..4387
                     /locus_tag="PLM68_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT55119.1"
                     /translation="MNKYRLIPLITVALLLAGCGIKGQNQQEAAPEKIVVKAAAVTLE
                     EVPQINEFTATVQANIINNIAPQMAARIKKIYVEVGDFVTKGQKLVQMDDNNLEQVRT
                     QLENMEISFRRVDELYKVGGVSQADWDAQKTRLDVMRTQYDNLVENTQLVSPIDGVIT
                     ARHYDSGDLFGMSMPLLVVEQISPVKLLVHVSESFFTKIRKGMEVDIRLDVYPGEVFK
                     GRVSIVYPTITAATRTFPVEIVIPNVNRKVRPGMFARVAIDFGAEPCLLIPDQSVIKQ
                     QGSGERHVYVYKDGKAEYRLVEPGRRLDSFYECLSGLEKGELVITTSLNRLTNGAEVE
                     LANE"
     gene            4397..>6083
                     /locus_tag="PLM68_06890"
     CDS             4397..>6083
                     /locus_tag="PLM68_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT55120.1"
                     /translation="MSIYQAAVRKPVTTALIYVGLAVIGIFSFSRLSVDFLPSISDNT
                     ILVVTTYSGASAADIENNVTKLLESTLSSVSDLKHITTRTKENSSLVMLEFEYGIGID
                     EAMNDVRDRLSLIADYLPSDANTPVLFKFGTDDIPIMILSVQARESMNGLYKILDDRV
                     ASPLSRIKGVGTVAIGGAFKREIQVYCDPYKLEAYNLTVEGIAATIAYENRNTPGGNL
                     DIGSETLTIRMEGEFDDPRQLKDIIVGYFQGRNIFLSDVARVEDTIKERSQESFIDGI
                     QGGTIIIQKQAGANTVQICKKVLEKLPELQKNLPSDISIGVVTDFSDNINRVIDSLVN
                     TIMITLILVVFIVFFFLGRWRATLIVAIVIPVSLISAFIYLLITGNTLNIISLSSLSI
                     AIGMVVDDAIVALENITTHIERGTKPKSAAIYATNEVSISIIASTLTLLAVFIPLTMV
                     TGMAGEIFKQLGWVVALVSSVSTIAALTLIPMMASQMLRLNSKKSKAFQKIYGPVQKF
                     LDALDVAYSRALNWTVSHRITVILVSAAIFLTSLLLFIKVPTEFFPQQDNARLS"
BASE COUNT         1644 a         1461 c         1469 g         1509 t
ORIGIN      
        1 ccgaaggagt gttcgcattc atgggcttta cggttattat tccagatctg atagggagcg
       61 gcatcagcgc acacgttccg caaccattcc tgtttatgga caataccggc cgggtatgct
      121 acgacatgca gcttgctgcc atggaatttt tcagcgctta tggtgccgca cctctttccc
      181 cggaaataga tattttcggt tattcagggg gagccacggg agccgtagcc ctgctccggc
      241 acattgacgg gctgcctgat tcccctgtca gggtgaaacg attgattgca ggagccggcg
      301 tctacgacat gagggaaaca tataaggttc tcaaagagcg tggttttgca gagtatcccc
      361 tggttcccct gatgataaga tccctggact actggtatga cctgcaattg gattactccc
      421 tggtttttac aggcgatttg cttgccaacc gcgaagaatg gctggacggg acaaggaatg
      481 ccagccagat gcgtgaactc ataagcccgc agctgaacac ctatatgcat cccgacttct
      541 tctccccgga aagcaattcc gaaattaaaa aaattgaaaa tgccctggct ttacattcca
      601 tgaaggaagg ctggaatacc cgcacccgaa tttacctggg ccatgcaacg gatgatatgt
      661 ctgtcccatt ttcagaaacg caggcgtttt tcaacagtat gaattcaaaa tcaaaaacat
      721 ccatgacaaa aggagccgga ggtcattatc actacggtat tgagtttttt atatccggct
      781 gccttttttt aacagccaat tgaacatgtc gcgttatttt aaagaagttc tcaaagaagg
      841 agacttttcc atcaccgatg tatttacaga agtaaaaaag gatttcccgg ccctggacct
      901 ttcacgtata gaacccctga tcagaacttt gttcaaaatg agggtgcgca taactgccat
      961 ttcatacaac accgtagatc ccctggaaaa tccggtgctg tcttccggta tcatcatgcg
     1021 tcccataaac aggaaacccc gcggtgttct tcattttcta ccctcggcca atatcgacaa
     1081 attcggatcg ggcagtgacg cgctcatact gtttgaagga gtcttgtcat tcttgggcta
     1141 tattgtcatc attcccgacc tgctgggtaa cggcatcact aaagatacgg tggaataccc
     1201 tttccttatg gcggaaaaca caggtcaggt ggcatacgac atgcaccggg cggccataga
     1261 atactaccag gaagtactgg gtcatcccct gccaaaacat atatctatcg ggggatattc
     1321 catgggcgga tcgggagcac tggcgctggt ccggcacatt gaacgggaga atccttcaga
     1381 tatcattgtt gacagggcca tcataggagg cggtttatac gatctgaatg tggcttttga
     1441 agaattcgca aaaaccggct ttgcgcaata tccggcagcc ccgggcgtct tcaaggcata
     1501 cgacaagtgg tacaatctga acctggacta ttcccgggtc tttaccggcc ccctgctgga
     1561 aaacatggac agctggctgg acaggacaca ctaccgggat cagttgaccg aatggatagg
     1621 tcaggacctt cacaaatata tgcaccccga tttctttact cccgaaagaa acagcgagat
     1681 caaaaaactc tgggataagt tcagggaaaa ctcgctggct gacggatgga ctccaaaaac
     1741 acatattctg atagcccatg cctctgacga tttgtctgtc cctacacgcg tggcaaccta
     1801 taccgtggaa caattcagaa aacgcggggc ccgtatccgc agtcgttatg ggaagggcgg
     1861 ccattacgag ttcggaaaat ggttctttct gcgcatgggg attcatttaa ttcttaaacg
     1921 gatggccttc tggacccgtt tttgaacatt ttcgtgcttt tttgctggtt tatttgtttt
     1981 tggcataaaa actgcactac tttgcctgct cgtattttat tgtaagttta tgaagaaatc
     2041 tgcctgtatt cttgttttga tattttcttt tgcgcaaggg caattactca gggcacagga
     2101 cacactgtat gtgaacctgg aaactgctct ggaaattgca ctttcagaga gtccggttat
     2161 ccggattgca gaccgtgagg tggaacgcgc tttttatgca cgtaaagaaa cccaatcggg
     2221 actctggcca aaaatctcgg ccagcggcat gtataaccgc accctcaaga agcaagtcat
     2281 ggtaatggat ttcggaggac aggaaatgga aatctctgta ggaacggata acaactggat
     2341 gggaggcgta tctttatcac tcccgctggt tgctcctgcc ttatggaaaa cagtacagct
     2401 ttcggctctg gatattgatc tggcagtaga gaaagcacgt tccggcaggt tagccatgat
     2461 cagcgccgtt aagcaagcat actactctct gctgctggcg aaggattcct atgtggtgct
     2521 aaagaaaaat tatgacaatg tggcactcaa caccaaaacc gttaccgaca aatatgaaca
     2581 ggggatggcc agtgagtttg acaaactccg tgccgaggtt gcccttaaga atcagcatcc
     2641 gaacctgctg gcctcggaaa cagccattga actggcctcc atgcaactca aagccctgat
     2701 gggccttgat attgagtatc ctgtggtctt tgaaggttcc ctgatccaat atgagggaga
     2761 ggtaactccc gggggtttgt cggcagccag tgagttttct ctggagaaca acactgacct
     2821 gatgcagctt gccatccaga agcaacaact ggaactggcc atgaagatca caaaagcatc
     2881 atttcttcct accctgagcc tttcttccaa ttatcaatat tcatccatga acaatgactt
     2941 ccgtttctcc aactataact ggtacccata ctctaccgcc tcccttgccc tgaacatccc
     3001 tttgttcagc ggaatgaaac ggcaacaaca agtccggcaa agcaaactga cactccagac
     3061 cctggaagac agccggatta attcagaaag gacactacgc ctgaatgtaa acaactgcct
     3121 taaccagatg ggcaatgccg tggaagaatt ctcttctaac aaagaaaacg tggccatggc
     3181 agaaaaggca tactccataa gccgcaaaca atttgaagtc ggaatgggga cgtggctgga
     3241 tctgagtgcc tcggaactgg ccctcacgca agcgcgtctg gcttatcacc aatctgtcta
     3301 cagctacctt ttctatagag cagaactgga aaaaataaca ggaacaagta attatcaaaa
     3361 tcaataacat atgaataaat acaggttaat tcccctgatc acggtcgcat tacttcttgc
     3421 aggatgtggt ataaaaggtc agaatcaaca ggaggctgct ccggaaaaga tcgttgtcaa
     3481 ggctgccgct gtgactctgg aggaagtacc ccagatcaat gaattcacag ctacggtaca
     3541 ggccaatatc atcaacaata ttgcgccaca gatggctgcc cgcatcaaga agatctatgt
     3601 ggaagtgggt gattttgtta ctaaaggaca gaaactggtc cagatggacg acaataacct
     3661 ggaacaggta cggacccaac tggaaaacat ggagatctct ttccgccgtg tggacgaatt
     3721 atacaaggtt ggaggcgtat cccaggctga ttgggatgct cagaaaacac ggctggatgt
     3781 catgcgtacc cagtatgaca acctggtgga aaatacacaa ctggtaagtc ccatagacgg
     3841 tgtgatcacc gcccgccact atgacagcgg ggatcttttt gggatgagca tgcccttgct
     3901 ggtggtagag cagatctccc cggtaaagtt gctggttcat gtttcagaat cattctttac
     3961 aaaaatcagg aaagggatgg aggtggacat tcgtcttgac gtttatccgg gtgaggtatt
     4021 caaaggaagg gtttccatcg tttacccgac cattaccgct gctaccagga cttttcctgt
     4081 agagatagtc atccccaacg tcaacagaaa agtcagaccc gggatgtttg cccgtgtggc
     4141 catagatttt ggggctgaac cctgcctgct tattcccgac cagagtgtca tcaaacagca
     4201 gggctcggga gagcgacacg tttatgttta taaggacggg aaagctgaat acaggctggt
     4261 ggaaccgggc agaagactgg actcgtttta tgaatgtctt tcaggcctgg aaaaagggga
     4321 gctggtcatc acgaccagtt tgaacaggct tacgaacggg gcagaggttg aacttgctaa
     4381 tgaataaagg aacattatga gtatttatca agcggctgtc agaaagccgg tcaccacagc
     4441 ccttatctac gtaggtcttg ctgtcatagg aatcttctcg ttttcaaggc tatctgtgga
     4501 ttttctaccc agcataagtg acaacaccat tctggtggtg accacgtatt ccggagccag
     4561 tgctgcagat attgagaaca atgtgaccaa gctgttggaa agcacgttga gcagcgtcag
     4621 tgaccttaaa catataacaa ccagaaccaa ggaaaactct tcgcttgtaa tgctggaatt
     4681 cgagtacggg ataggcattg acgaggcaat gaacgatgta agggacaggt tgagcctgat
     4741 cgcagactat ttgccctcag acgccaatac ccccgtactt ttcaagtttg gtaccgatga
     4801 catacccata atgattctgt cggtccaggc ccgggaaagc atgaacggcc tgtataaaat
     4861 tctggatgac cgggtggcct ctcccctgag caggataaaa ggggtaggaa ctgttgcaat
     4921 tggtggtgca tttaaaagag aaatccaggt atactgtgac ccctataaac tggaagcgta
     4981 taacctgaca gtggaaggca tagctgcaac catagcctat gaaaacagga acactccggg
     5041 cgggaacctt gatattggct cggaaaccct gaccatcagg atggaaggcg aatttgatga
     5101 cccacgccag ttgaaagaca tcattgttgg ctactttcag ggccggaata tctttctgag
     5161 cgatgtagcc cgggtggaag acactatcaa ggaaagatcc caggaatcct tcattgacgg
     5221 gatacaggga gggactatca ttattcagaa acaggcagga gccaacacgg ttcagatctg
     5281 taaaaaagtg ctggaaaagc ttccggaatt gcaaaagaac ctcccctccg acatcagcat
     5341 aggagttgtt acggactttt ctgacaacat caaccgggta atagatagcc tggtaaatac
     5401 catcatgatc accctgatcc tggtcgtatt cattgtgttc tttttccttg gacggtggcg
     5461 tgctaccctt atcgtggcaa tagtcattcc cgtgtccctg ataagtgcat tcatatacct
     5521 gctcataacc gggaataccc tcaatatcat ttctctttcc tccctgagca ttgccattgg
     5581 gatggtggtg gacgatgcca tcgttgccct ggaaaatatt acaacccaca tagaacgggg
     5641 aacaaagcca aagtcggctg caatttatgc caccaacgag gtatccattt ccatcatcgc
     5701 ctctaccctg acattgctcg ccgtgttcat tcccctgacc atggtcactg gcatggccgg
     5761 agaaattttc aagcaattgg gctgggttgt ggccctggtg agttctgtat caaccatcgc
     5821 tgccctgacc ctgatcccca tgatggcttc ccagatgctc cgtctaaatt ccaaaaaatc
     5881 aaaagcattt cagaagatat acgggcctgt gcagaaattt ctggatgcac tggatgttgc
     5941 ctactccaga gccttgaact ggacagtcag ccaccgtatt accgttattt tggtctcggc
     6001 ggcgatattt ctgacttccc tgctcctgtt tattaaggtt cccaccgagt ttttccctca
     6061 gcaagacaac gccaggttgt cca
//
LOCUS       DAOSOF010000116         6074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_13119_length_6074_cov_4.692308, whole genome shotgun sequence.
ACCESSION   DAOSOF010000116 DAOSOF010000000
VERSION     DAOSOF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13119_length_6074_cov_4.692308"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLM68_06895"
     CDS             <1..861
                     /locus_tag="PLM68_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55121.1"
                     /translation="TMEKNLKTIIIILSVVAALLIGALAWVWIDRGGMIKELNLEKED
                     LTQQTIQLRSQYDSLSTTNDTLNLRLMEEREKIDLLIERINKTEATNRTRIREYEKEL
                     GTLRSIMRSYIHQIDSLNTLNLSLRQDASQAREEASRATQQYEELRTTTDEYARQVEI
                     GAQVKGRNFILTGITETGKDSDRSSRITKLKLCGSLIENSIAQKGPRNIYLRIKGPDG
                     ILLTNPDQGVFNAAGEQLVYSAVREVDYQGVELEFCIFFGNVTFSRGIYTAEAFTTDG
                     QIGVADILLR"
     gene            858..2009
                     /gene="hemW"
                     /locus_tag="PLM68_06900"
     CDS             858..2009
                     /gene="hemW"
                     /locus_tag="PLM68_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT55122.1"
                     /translation="MIYVHIPFCRSFCSYCNFYSSTKLREIPPVLEFIEQEWIRERDT
                     WLDLWRSGAYRLRHTVYLGGGTPSVCTAEELEPLLQLLCNDLTMAGMAPVEITLEANP
                     GDLTPAYCTALLELGVNRLSLGVQSFEDTHLRAMNRRHTAGEGINAVKMARSAGFRNI
                     SLDLIFGFPGLTISKWEETLWQAVALAPEHISAYQLSLEPSSPWGKQGQLPPQEECAA
                     QYGLLQEFLAGRGYIQYEVSSFCLPGKESLHNSGYWIRTPYLGLGPAAHSFNGRDRYA
                     NVAHLGRYVQGMEKGSPKRTYDCLTPQDEHNEWVMLQLRTVEGFSTRGLQEREGAEAV
                     ADFLKQIAPLLERGLLELCCQDQGNARVRIPPGRLFVSDDIIRDCLLPV"
     gene            complement(1981..2550)
                     /locus_tag="PLM68_06905"
     CDS             complement(1981..2550)
                     /locus_tag="PLM68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOT55123.1"
                     /translation="MIIVSDKILVSENILTEYFTCNPRACQGICCVEGESGAPLEQDE
                     CTLLETEWTRFVPFMQKTGVRAIEKQGPWTLDIEGDAVTPLIEGKECAYAFFRDGICR
                     CAIEKAYSEGVSSFRKPVSCWLYPIRVRKLADGVIGLNYHKWYLCGAARELGAKKKTR
                     VFEFLKEPLTHCFGPDVYQAIRQAADNPG"
     gene            complement(2547..3023)
                     /locus_tag="PLM68_06910"
     CDS             complement(2547..3023)
                     /locus_tag="PLM68_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HOT55124.1"
                     /translation="MKKIVFFTLILAGLASCQNATDVPQLELTLSKEIKKNTELSTFI
                     MQAKDNANELARRCVEMHEKAQQYLTVNFDSLDTAQQEQLVKLDYEYVEMWYKFNVKF
                     TEQTVQLMEYLKDESIPKEELIEMGKSIAQVNTFVQELKDTYGQDLKLDPYPAPVQ"
     gene            complement(3105..3956)
                     /locus_tag="PLM68_06915"
     CDS             complement(3105..3956)
                     /locus_tag="PLM68_06915"
                     /inference="COORDINATES: protein motif:HMM:NF028271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT1926 family outer membrane beta-barrel
                     protein"
                     /protein_id="HOT55125.1"
                     /translation="MIKKLSIFLIAVLTVTSLGAQPAERSGSAVYAPRAGQVQVSAVL
                     GSGLYFEYLDNLTPLLPSYMPGDELGIDPTSYGVGYYLQMGNMNYNPIVNLAGIQGKV
                     FLTNRFALNAMFAMQIDVTPQRDYIEGINLESSGLLDLGGIQWVEGRLTNKYLVNVGA
                     DYYFNNCNGRLFPYIGVLGGFQHARVQAYLPYTGIEVPTETGTDPIELYALDNRAGEA
                     YAISGAITAGVEYNLLPGMNIALEVRPFNYYYTAIRMYADGQEFNAYEMQFKFFAFPQ
                     LKIGFRF"
     gene            complement(3981..>6074)
                     /locus_tag="PLM68_06920"
     CDS             complement(3981..>6074)
                     /locus_tag="PLM68_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55126.1"
                     /translation="FSDGGDDPNIHVNNGTYRLAKWGWATTPSMFDIMKRDMRVIDDT
                     RKEFEDQESGIIEANIAAKKKAAAEATTAYTTNWNNWQKYYKEGQPGAGARYTAWLDA
                     WNAWNTANQNFLAHQTTYENMYYEADSLIRLFMNYLDGWLNLGNLEVPSTLTLSQVLG
                     GIEFNAGTLADWLLQGNADDVFETINVIIGLINAPTEMDAIITQLKGIMDGTHPDGFP
                     PFWQHATAWPYEKTPYPFNDIMRKVYANSDKELTDLTKEDWYAWLFLYWLFESRTVEI
                     GLENSNGGSLITINAAEYTDAAQEVLEDFFGELTPKVHKKIVKPDSDVAYTGPYYVDY
                     MKGTGTKALENAIKAYFDAVEGLGNMETAMYDPFDRVWKAYAKHKDANGGVEFEMTRT
                     LALGTYLLWKPFLTRIAPTPGTYNPPTYNDTPDLTETQPAPIDFNQFFFVLEAYKDYT
                     SPSPSLDFDITYLDDDCNLIVSYAALKAAGDDCGKQWSEWVGDMLLWEWDGPTYANGH
                     YFYAIYQARDYQAYQDTYNRVKNGEYEALIGKIQALYDAQLTAFNSAKAYAASLQAEL
                     DAIEDGMEEAQDMIEAYECYIDEYEWLIAQAMMAHDFGNWLYTDADGGLLDLWEDAKH
                     QLMELQQDVAAIEQIMALLEQGLPAPDLWGDLIQDRTVLYQAKIDAILLDIENLQQKL
                     AILEGIRAKLLEDYL"
BASE COUNT         1454 a         1511 c         1530 g         1579 t
ORIGIN      
        1 actatggaaa aaaatctcaa aaccattatc attatcctgt ccgtagtagc cgcactgctg
       61 ataggagccc tggcctgggt gtggatagac cggggtggca tgataaaaga actgaatctg
      121 gaaaaagagg acttgactca gcagaccatt cagttgcgct ctcaatacga ttcacttagc
      181 actaccaacg acaccctgaa cctgcgattg atggaagaac gggaaaagat cgatctgctt
      241 attgagcgta tcaataagac agaagccacc aaccgcaccc gtatacgtga atacgaaaaa
      301 gaactgggaa ccctgcgcag tatcatgcga agttatatcc accagattga ctcgctcaat
      361 accctgaacc tgtcgttgcg gcaggatgcc tcacaggcaa gggaagaagc ttcccgcgcc
      421 acgcagcaat acgaggaact ccgcaccacg accgatgaat atgcccggca ggtagaaata
      481 ggggcccagg tcaagggtcg taatttcata ctcaccggca ttacagaaac cgggaaagat
      541 tccgaccgtt ccagccgaat caccaagctg aaactgtgcg gaagcctgat tgagaattcc
      601 attgcacaaa aagggcccag gaacatatac ctgcgcatca agggaccgga cgggatactg
      661 ctcaccaacc cggatcaggg tgtgttcaat gctgccggag aacaattggt ttactcggcc
      721 gttagggagg ttgactacca gggcgtggag ctggaattct gcattttctt tggcaatgtg
      781 acattcagcc gtggcattta tacggcagaa gccttcacca cggacggaca gattggcgtg
      841 gccgatatcc ttctgcgttg atatacgttc acattccgtt ttgcaggagc ttttgctcct
      901 attgcaattt ctattccagc acaaaactcc gggaaattcc cccggtgttg gaattcattg
      961 aacaggaatg gatacgtgaa cgcgatacgt ggcttgattt atggcgcagc ggagcatacc
     1021 gcctgcgcca tactgtttat ttagggggcg gaaccccctc cgtgtgtacc gcagaagaac
     1081 ttgagccgct cctgcaattg ttgtgcaatg acctgacaat ggccggtatg gcgcctgtgg
     1141 aaatcactct ggaagccaat cccggagacc tcacgcctgc atactgcact gcattgctag
     1201 agcttggggt gaacaggctg agcctgggag tgcagtcctt tgaagacacc cacctccggg
     1261 ccatgaatcg ccgtcatacg gccggggagg gcatcaacgc cgtaaaaatg gcccggtcag
     1321 ccggttttag gaatatcagc ctggatctga tctttggatt tccgggcctg accatatcca
     1381 aatgggagga aaccctgtgg caggcggttg ccctggctcc tgagcacatc tcggcttacc
     1441 agctgagcct ggagccctct tccccctggg gaaaacaagg ccagctgcct ccccaggaag
     1501 aatgtgccgc acagtacggt ttgctgcagg aattcctggc cggccgggga tatatccaat
     1561 acgaagtatc cagtttctgc ctcccgggca aggaatcact gcataacagc ggatactgga
     1621 tccgtactcc ttaccttggc ctgggacctg ctgcccattc attcaacggt cgcgaccgct
     1681 atgccaatgt ggcgcacctg ggccgttatg tgcagggaat ggagaagggc tcacctaaaa
     1741 ggacttacga ctgtcttaca ccacaggatg agcacaatga atgggtcatg ctccagttgc
     1801 gcacggtgga gggattttcc acaagagggc tgcaggagcg ggaaggagca gaagccgtgg
     1861 cggatttcct gaaacaaata gccccgctgc tggagcgggg ccttctggaa ttgtgctgtc
     1921 aggatcaggg caacgcccgg gttcgcattc ccccgggccg gctgtttgtt tctgacgata
     1981 ttatccggga ttgtctgctg cctgtctgat ggcctggtaa acatccggtc caaagcagtg
     2041 ggtcagcggt tctttcagga attcaaaaac acgtgtcttt ttcttggcgc ccagttcacg
     2101 ggccgcaccg cataaatacc acttgtggta attgagtcct atgacaccat cggcaagttt
     2161 ccggacccgt atgggataga gccagcacga aacgggcttt ctgaaagagg aaacgccctc
     2221 gctgtatgcc ttttcaatgg cgcaccggca gataccgtcc ctgaaaaaag catacgcaca
     2281 ttctttccct tcaatcaggg gggtaaccgc atctccttcg atatccaggg tccagggacc
     2341 ctgtttttct atggcacgaa ctcctgtctt ttgcataaag ggcacaaatc gtgtccattc
     2401 cgtttccagc aaggtgcatt catcctgctc caggggcgcc ccgctttctc cttccacgca
     2461 gcaaatgccc tggcaggccc ggggattaca ggtgaaatat tcggtcagga tgttttcact
     2521 gaccagtatc ttatcagaga caatgatcac tgtaccggtg ccggataagg gtccagtttc
     2581 aggtcctggc cgtaagtgtc ctttaactcc tggacaaagg tattgacctg ggcgatggat
     2641 ttgcccattt ctatcagttc ttctttggga atggactcgt ccttaaggta ttccatcaac
     2701 tggactgtct gttcggtaaa cttgacattg aatttatacc acatttccac gtattcgtaa
     2761 tccagtttga caagctgctc ttgctgcgcg gtatccaggc tgtcaaaatt aactgtaagg
     2821 tattgttgtg ccttttcgtg catctccaca catctgcggg caagttcatt cgcattatcc
     2881 tttgcctgca taatgaatgt ggaaagttcg gtattctttt ttatctcttt ggacaaggtc
     2941 agctcaagct gaggaacatc ggtggcattt tggcaggaag caagtccggc caggatgagt
     3001 gtgaaaaata caattttctt catggtaatt agtgatttga ggtacaaagg taagaaaaaa
     3061 ccgcccccat tgggaggcgg ttttacaaat tcgttcaaca attactaaaa acggaatcca
     3121 attttaagtt gcgggaaagc aaagaatttg aattgcattt cataagcatt gaattcctgg
     3181 ccgtctgcat acatacggat tgctgtgtag taataattga aggggcgcac ctcaagagct
     3241 atgttcatgc ccggcaggag attgtattcc acgccggccg tgatcgctcc ggatattgca
     3301 tatgcttcac cagcacggtt gtccaacgcg tagagctcaa tgggatctgt cccggtttct
     3361 gttggcactt ctatccctgt atagggcagg tatgcctgaa ctcttgcatg ctggaaacct
     3421 cccagtacac caatgtaggg gaacagcctt ccgttgcagt tgttgaaata gtagtctgcg
     3481 ccaacattta caaggtattt gttggtcagc ctgccttcaa cccactgaat acctccgagg
     3541 tccaaaagac cactgctctc caggttgatg ccttcaatat aatcacgctg gggggtaacg
     3601 tcaatttgca tggcaaacat ggcgttgagt gcaaagcggt tggtcaggaa cacttttccc
     3661 tgtatccctg caagattgac aattggattg taattcatat tgcccatttg cagataataa
     3721 cccacaccat agcttgtcgg atctatgccc agttcatcac ccggcatata acttggcaac
     3781 aggggggtta gattatccag atactcgaaa tataatccgc ttcccaatac cgccgagact
     3841 tgcacttgtc ccgcacgtgg agcatataca gcacttccgg agcgttctgc cggttgcgcc
     3901 ccaagagacg taaccgttag aacagctatc agaaatatgc taagtttttt tatcattata
     3961 cgcaaactta tccgtatggt ttatagatag tcttccagta atttggcgcg gatgccttcc
     4021 aggatagcga gcttctgctg caggttttct atatctaaca ggatagcatc tatcttggcc
     4081 tgatacagaa cagtcctgtc ctggataaga tctccccaaa ggtcaggagc gggcaaaccc
     4141 tgttccagaa gggccatgat ttgttcaatg gctgcaacat cctgctgcaa ttccatcagc
     4201 tggtgcttgg catcttccca caagtcgagc agaccgccat cggcatcagt atacagccag
     4261 ttcccgaagt catgagccat catggcctgg gcgatcagcc attcgtattc atcaatatag
     4321 cactcatagg cttctatcat gtcttgggct tcttccatac cgtcttcaat ggcatccagt
     4381 tcagcctgca gggaggcagc gtaagccttt gctgagttga atgctgtcaa ttgtgcatca
     4441 tacaatgcct ggatctttcc gatcaaagct tcgtattctc cattctttac ccggttgtaa
     4501 gtatcctggt atgcctggta atcacgggcc tgatagatag catagaagta atggccatta
     4561 gcatatgttg gtccatccca ttcccagaga agcatgtccc ctacccattc tgaccattgc
     4621 tttccgcaat catcaccggc tgccttcaag gcggcatagg aaacgatcag gttgcagtca
     4681 tcgtccaggt aagttatgtc aaaatcaagg gaggggctgg gtgatgtata gtctttatag
     4741 gcttccagca caaagaagaa ttgattaaaa tcaatggggg caggttgtgt ttcggttaga
     4801 tccggtgtgt cgttgtatgt cggcgggtta taggtgccgg gggtgggagc gattctggta
     4861 aggaatggct tccatagcaa gtatgtgccc aatgccaggg ttctggtcat ttcaaattcg
     4921 acaccgccgt tggcgtcttt atgttttgca taagctttcc ataccctgtc aaaaggatca
     4981 tacatggcag tttccatgtt tcccaggcct tcaacggcat cgaagtaagc cttgatggcg
     5041 ttttccaggg ccttggtgcc cgttcctttc atatagtcga cataataggg tccagtataa
     5101 gcaacatcgg aatctggttt tactatcttt ttatgaacct tgggtgtgag ttcgccaaag
     5161 aaatcttcca aaacctcttg ggcagcatcc gtatattcgg cggcattgat ggtgatcagg
     5221 gaacctccgt tggaattttc cagtcctatc tcaaccgtcc tggattcaaa aagccaatac
     5281 aggaacagcc aggcgtacca gtcttccttc gtcaaatctg taagttcctt atccgagttg
     5341 gcataaactt tacgcatgat atcattgaag gggtacgggg ttttctcgta gggccatgcg
     5401 gtggcatgtt gccagaaagg agggaatcca tcagggtgtg tcccgtccat gatgcccttg
     5461 agctgggtga tgatggcatc catctcggtg ggggcattga tcaggccaat gatgacgttt
     5521 atggtttcaa acacatcatc tgcattgccc tgcagcagcc agtcggccag ggtgccggca
     5581 ttgaattcaa taccgcccag aacctgtgat agtgtcaggg ttgaaggaac ttcaaggttg
     5641 cccagattca gccatccgtc cagataattc atgaacagtc tgataaggct gtcggcttca
     5701 taatacatgt tttcataagt ggtctggtgg gcaaggaaat tttgattcgc tgtgttccag
     5761 gcgttccagg cgtctagcca tgccgtgtaa cgggcacctg ctccgggttg tccttccttg
     5821 tagtatttct gccaattgtt ccagtttgtg gtgtatgcgg ttgttgcctc ggcagcggct
     5881 ttctttttgg cggctatgtt tgcttcgatg ataccggatt cctgatcttc aaattctttg
     5941 cgggtatcat caatcacgcg catatccctc ttcatgatgt cgaacatgct gggggtagta
     6001 gcccatcccc attttgccag tctataggta ccattgttga catggatgtt ggggtcgtct
     6061 ccgccatcgg agaa
//
LOCUS       DAOSOF010000117         6011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_13329_length_6011_cov_4.153123, whole genome shotgun sequence.
ACCESSION   DAOSOF010000117 DAOSOF010000000
VERSION     DAOSOF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13329_length_6011_cov_4.153123"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLM68_06925"
     CDS             complement(<1..328)
                     /locus_tag="PLM68_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOT55127.1"
                     /translation="MEENKQNLQPVEEDTIDLVEILQKLWAKRKFILIVTGVAMALGL
                     VVALLTPNQYTASCTLVPQTGERRTGGSLSGLAAMAGISLGDMTSGEVLSPNVYPNII
                     KNVEFQK"
     gene            complement(336..2735)
                     /locus_tag="PLM68_06930"
     CDS             complement(336..2735)
                     /locus_tag="PLM68_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOT55128.1"
                     /translation="MRIFRTLLSTAVFLLVAVVAYGQLSDQQVISEVKRLQATGASQQ
                     QILTELAAKGVTREQAERIYADYQAQGATQAGTVVNTESRTRELSEPVEPEVIVVQET
                     PSRGPISVFGKNLFASKNLTFQPNMNMPTPENYELGAGDEVIIDIWGNSELTVRQEIS
                     PDGYINVTNIGPIYLQGMDIKEASAKIKTAFGRIYSDLRSANPRTFIQISVGNVRTIK
                     VNIMGEVERPGTYSLSSFASVFHALYAAGGINEIGSLRDIKVYRNGKLQVQVDIYEYL
                     LRGDSSKDIILKENDIVKVEPYNNRVQITGQVKRPMIYEMMGDETLAQLIGYAGGFAS
                     DAYKRNVQLVRHGVTERRMFTVEMTDYESFVMQDGDSVSVGGILERYENRVEIAGAVF
                     RPGTYAIDQRVQTLLDLVTVAEGPREDAFLGRAILNRQNPDLSRTVESVDLGKLLSGE
                     FADIPLKKNDRFYVMSEDSLRQEYTVRLEGEVMNPGDYPYADNMSIEDLIITGGGLRE
                     SASHIKIDVARRTRNALAREESMDMSEIYSFSIENGLVISGDKDFVLKPFDIVTVRRS
                     PGYKEQERVTMQGEVVFPGNYSKQHNNERISSFIKRAGGLTPSAYSKGATLLRQMDER
                     EQNMTDAALRLSIQQSRDSIVVDSVDRIKEGYYVGIDLEKIMESPGRNGDIVLREGDV
                     LDIPILNNVVKISGGVMYPNAVAYRDNMTLGDYIDNAGGYVRGARRSKVYVVYMNNTV
                     SKGRGSKIEPGCEIIVPVKPERQGASWGDIVGVTTSVLSVISLTVATLLNVSRKNPTS
                     N"
     gene            complement(2763..3878)
                     /locus_tag="PLM68_06935"
     CDS             complement(2763..3878)
                     /locus_tag="PLM68_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT55129.1"
                     /translation="MRSAPVVAILGPRQCGKSTLVKNMFPESANTVYLDLERPSDLSK
                     LEDAEWYLSMQKGKLICIDEIQRKPELFPLIRFLADQWSVNGVFLVLGSASRDMLKQS
                     SESLAGRIHYKKLTPFLLEEIENKYTLYDYFAKGGFPRSILAEDEKLSFQWRQDFIST
                     FLERDLLQWKGFTPATMRRLWQMLAHNNGKTADYSTFAKSLNVSSVTVKNYIDLLEST
                     FMVEVVAPYISNMGKRLVKAPKVYISDSGITAALLGLYNFDALSAHPSFGTIWEQIVM
                     SNLKGVFPEAEFYFYRTANGAEIDFVMKIYDSVFAIECKSGLSPTLSKGNYNAIEDIS
                     PTHTFVVLPIEKGWPMKQGIDAVSLTEVKEKLPTFIR"
     gene            complement(3980..4321)
                     /locus_tag="PLM68_06940"
     CDS             complement(3980..4321)
                     /locus_tag="PLM68_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55130.1"
                     /translation="MNKTTETEERTLKEIQQAIERVDRERLDPNLGVKERGLLELSAL
                     ALRDAERLAIARLQKQVYKDMEQVTARLNQLSKRIRSRVTRMNKVPKALDAIETVIKE
                     VVKILVAVGKW"
     gene            complement(4318..5577)
                     /locus_tag="PLM68_06945"
     CDS             complement(4318..5577)
                     /locus_tag="PLM68_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT55131.1"
                     /translation="MKSVVLQQKEERDALLRNVYQKRVLIESTEQYLESGLIKLITGP
                     RRAGKSVFALQLLGSKNFAYLNFDDELLLSSFDENAILQALQEVYPQFQYLLLDEIQN
                     LPRWETWVGKLYRRGVNLVITGSNARLLSGEMATMLTGRYLQIEILPFGLKEVLDFLQ
                     VALLAQTPVQKANVMLQVEDYLVFGGFPETLSLRSITRNYLSALFDSILLKDITKRYK
                     VRNTNELYNLASYLLANYGNPFSLTRLVDDLNLGSKVTAQKYCLYLEETYVFFYLPRF
                     NNKLKQMLKAPLKPYIVDNGFIAARSFEQSPNRGRLLENTVIVELIRRGYKPGQTLFY
                     YRSRNNKEVDFVCRKGAQVDQLIQVSYDVNNPRVLKREISALLECSGELHCNKLLLLT
                     WDKEITIEQERQTIQVIPVWKWMTEEK"
     gene            complement(5640..>6011)
                     /locus_tag="PLM68_06950"
     CDS             complement(5640..>6011)
                     /locus_tag="PLM68_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55132.1"
                     /translation="DTLVAVCVDALVQLLRKQEVQELIDNEETGLKNNYLAYLQRVEA
                     LGQLPPVDHDRRYMTLLEDLEKAQKIRDKCVSGLQSLKRAHHKLLTQLDKRERWDYWY
                     EELTELNTLAVQLSTLIKELK"
BASE COUNT         1463 a         1514 c         1257 g         1777 t
ORIGIN      
        1 ctttctggaa ttccacgttt ttgatgatat tgggatagac attgggggaa agcacctcgc
       61 ccgaggtcat gtctcccagg ctgatcccgg ccatggcggc caggccgctc aaactgcccc
      121 cggtcctgcg ttctccagtc tggggcacca gcgtacagct ggcggtgtat tggttggggg
      181 tgagcaatgc cactaccaat cccagggcca tggccacacc cgttacaatg agtatgaatt
      241 tccgtttggc ccagagtttc tgcaagatct ccaccaggtc aatggtatct tcttctacag
      301 gttgtaggtt ttgtttgttt tcttccatgc ctgttttaat tactggttgg gtttttacgg
      361 gaaacattca acaaggtagc cacggtcagt gaaatgaccg acaacacgga agtggtcaca
      421 cccacaatat ccccccagga agcaccctgg cgttccggtt taaccggtac gataatctcg
      481 cagcctggtt caattttgga accgcgtcct ttggaaacgg tattgttcat atagaccacg
      541 tacactttgc tccggcgagc acctctaaca taaccccctg cattgtcaat atagtctccc
      601 aaagtcatat tatcccgata cgccacggcg ttgggataca tgaccccgcc cgagattttc
      661 accacgttgt tcagtatggg tatatccaac acatctcctt cccttaagac gatatctccg
      721 ttcctgccgg gactttccat gattttttcc aggtctattc ccacataata cccttctttt
      781 atccggtcca cactatccac cacaatggaa tccctggatt gctggatact cagccgcaag
      841 gccgcatcgg tcatgttctg ttctctttca tccatttgac gcaacagggt ggctcctttg
      901 gaataggcac tcggagtcaa tcctccggct ctttttatga atgaagaaat gcgttcgttg
      961 ttgtgttgct tggaataatt gcccggaaaa accacttctc cctgcatggt gaccctttcc
     1021 tgttctttat aaccgggaga acgtcttacc gtcacaatat caaatggctt caagacaaag
     1081 tccttgtccc cggatatcac caatccgttt tctatggaga aagagtatat ttcactcata
     1141 tccatagatt cttcccttgc cagggcattg cgggtacgac gagccacgtc aattttgatg
     1201 tgtgaggccg attccctcaa accgcccccg gtgatgatca ggtcctctat gctcatatta
     1261 tcggcatacg gataatctcc cgggttcatg acttcacctt caagccttac ggtatattcc
     1321 tgccgcaggg agtcttcgga catgacataa aaccgatcgt ttttcttcaa gggtatgtca
     1381 gcaaactctc cggaaagcaa cttgcccagg tccaccgatt ctacggttct ggacagatcg
     1441 gggttctggc ggttcaggat ggctcggccc aggaaggcat cttcccgggg tccttcggct
     1501 acggtaacca ggtccaggag tgtttgcacc cgctggtcta tggcgtacgt acccggccgg
     1561 aaaacagctc cggcaatctc cacccggttc tcgtagcgtt ccaggatgcc ccctacactt
     1621 accgaatccc cgtcctgcat tacaaacgat tcataatcgg tcatttctac ggtaaacatc
     1681 cgtcgttcag tcaccccgtg acggaccagt tgcacattcc ttttgtaggc atcactggca
     1741 aaacctccgg cataaccgat caactgtgcc agcgtttcgt cccccatcat ttcatagatc
     1801 atggggcgtt ttacctgtcc ggtgatctgt acccgattgt tatacggttc cactttcaca
     1861 atgtcgtttt ctttcaggat aatatccttg ctgctgtcgc cccgcaacag gtattcgtaa
     1921 atgtcaacct gtacctgcag ttttccgttg cggtacactt ttatatcccg caaacttccg
     1981 atttcgttga ttcctcctgc cgcgtacagg gcgtgaaaga ccgaggcaaa ggaagacaag
     2041 ctgtatgttc cgggtcgctc cacctcgccc atgatattga ctttgatggt gcgcacattt
     2101 cccaccgaga tctgaatgaa cgtccgtggg ttggccgacc gcagatcgga atagatccgg
     2161 ccgaaggccg ttttgatctt ggcgctagct tccttgatgt ccattccctg caggtagata
     2221 gggcctatgt tggttacgtt gatgtacccg tccgggctga tctcctggcg tacggtcagt
     2281 tcgctgtttc cccatatatc tatgatgact tcatcccccg ctcccaattc gtaattttca
     2341 ggggtgggca tgttcatgtt gggctggaag gtcaggttct tggaagcgaa caggtttttc
     2401 ccgaaaaccg atattggccc gcgcgaaggt gtttcctgca ctacgatgac ttccggttcc
     2461 acaggttccg aaagttcccg ggtgcggctt tccgtattca caaccgttcc tgcttgggta
     2521 gcgccctgtg cctggtaatc ggcatagatc cgctcggcct gttcccgtgt aacgcctttg
     2581 gccgccagtt cggtaagtat ctgttgctgc gaggccccgg tggcctgcaa acgctttact
     2641 tcgctaatga cctgctggtc actcagctgt ccgtatgcca ccactgccac caacaaaaac
     2701 acggcagtac taagaagggt tctaaaaatt ctcatatgaa aaacgtttgt ataaatcttt
     2761 aattatctta taaatgtagg caatttttct tttacctcag taagagaaac tgcatctatt
     2821 ccctgtttca tgggccatcc tttttcaatg ggcaaaacaa caaatgtatg tgttggcgaa
     2881 atatcttcta ttgcattata attgcccttt gaaagcgttg gtgaaagacc cgatttacac
     2941 tctatagcaa atacagagtc atatattttc attacaaaat caatttcagc tccgtttgcc
     3001 gttctgtaaa aataaaattc cgcttccgga aatacccctt tcaaatttga cattacaatt
     3061 tgttcccaga tagtaccaaa tgacggatgg gcagagaggg catcaaaatt atatagacca
     3121 agcagcgcag cggtaattcc cgagtcagaa atatacactt ttggtgcttt cactaaacgt
     3181 ttacccatat ttgaaatata cggagccaca acctccacca taaatgtgct ttccaataaa
     3241 tctatataat tttttactgt aacagatgat acatttaatg atttcgcaaa agttgaatag
     3301 tctgctgttt tcccattatt gtgcgccaac atttgccaca accgtcgcat ggtcgcagga
     3361 gtaaatcctt tccattgcaa caagtccctt tctaagaaag tagaaataaa atcctgtcgc
     3421 cattgaaaag ataatttttc gtcttccgcc aaaatgctgc ggggaaaacc tcccttagca
     3481 aagtaatcat atagagtgta cttgttttct atttcttcca gcaaaaaagg ggtcaatttt
     3541 ttgtagtgta tcctgcctgc aagcgactct gaagactgtt taagcatatc gcgcgaagcc
     3601 gagcccaaaa ccaaaaagac tccattcaca ctccattgat ctgccaaaaa tcgtatgaga
     3661 ggaaacaact ccggtttgcg ttgaatctca tcaatacata tcaacttgcc tttttgcata
     3721 gataaatacc attcagcatc ttccagtttt gagagatcgg aaggacgttc aagatctaga
     3781 taaactgtgt ttgcactctc tggaaacatg tttttcacca atgtagattt tccacattga
     3841 cgaggcccta aaatagctac aacaggagcg cttctcatag attgtatgat acttttctct
     3901 atggatcttt gaatgtacat tcaatacgtt acattatttt accatgcaaa tataaaacta
     3961 tattttaaat ttacctgcat taccacttac ccacggccac caggatcttc accacttctt
     4021 tgatcaccgt ttcaatggca tccagggcct tggggacttt gttcatgcgg gtgacccggc
     4081 tgcggatgcg tttggacaat tggtttaacc gggccgttac ctgctccatg tctttgtaca
     4141 cttgtttctg caaccgggca atggccaacc gctcggcatc ccgcaacgcc agggcggaca
     4201 gctccagcaa cccccgttct ttcaccccca ggttgggatc cagccgctcc cggtccaccc
     4261 gttcaatcgc ttgctggatt tcttttaatg tacgttcttc tgtttctgtt gtcttattca
     4321 tttctcttca gtcatccatt tccataccgg tattacttgt atggtttgac gttcttgttc
     4381 aatggttatt tctttatccc atgtaagcag caatagctta ttgcaatgca attcaccgga
     4441 acattccagt aatgcggaaa tttcccgttt cagtaccctc ggattattca catcatagga
     4501 tacctgaata agttggtcca cctgagctcc tttccggcaa acaaagtcca cctctttatt
     4561 gtttcttgat cggtagtaaa acagagtttg ccctggtttg tatcccctcc tgataagctc
     4621 aactatgacc gtgttttcca acaaacggcc tctatttggt gactgttcaa aggaacgggc
     4681 tgcaatgaat ccattatcaa ctatatatgg tttcagcgga gcttttagca tctgttttag
     4741 tttgttatta aaacggggca aataaaagaa cacgtatgtc tcttccaaat agaggcaata
     4801 cttctgtgct gtcaccttac tgccaaggtt caaatcgtca accagccggg tcaatgaaaa
     4861 cggattccca taattggcta ataaataact ggcaaggttg tatagttcgt tggtgtttct
     4921 taccttatag cgtttggtta tgtctttaag aagaattgaa tcaaataagg cagaaaggta
     4981 atttcttgtt atacttctca gggataaggt ttcaggaaaa cctccaaaca ccaggtaatc
     5041 ttcaacttgc agcatcacat tggctttttg aaccggtgtt tgggcaagca gtgcgacctg
     5101 aagaaagtcc agcacttctt tcaatccgaa tggcaggatt tcaatttgca aatagcgtcc
     5161 tgtcagcatg gtggccattt cgcccgaaag taatctggcg tttgatccgg taattacaag
     5221 gttaactccc ctccggtata gctttcctac ccatgtttcc caacgtggta gattttgtat
     5281 ttcatccaga agaaggtact gaaattgagg ataaacttcc tgtaaagcct gtaaaattgc
     5341 gttttcgtca aaagaagaaa gcaataactc gtcgtcaaaa ttcagatagg caaaattctt
     5401 tgaacctaac aattgcaatg cgaatacaga ttttcctgcg cggcgcggcc cggttatcag
     5461 ctttataaga ccggattcaa gatattgttc ggtagattct atcaaaacac gtttttgata
     5521 gacatttctt aacaacgcat cccgctcttc tttttgctgt aaaacaaccg atttcattca
     5581 ttaaaataat tgtactgaac aaatttaatg ttttttgttc agtataaaaa aaatactgtt
     5641 tatttcagtt ctttaataag ggtgctcagt tgcacggcca gggtattgag ttctgtaagt
     5701 tcttcgtacc agtagtccca acgttcccgt ttgtccagtt gggtaaggag tttgtggtgg
     5761 gcccgtttga ggctttgcag cccgctcacg catttgtccc tgattttttg ggctttctcc
     5821 aggtcctcca gcagtgtcat gtaacgccgg tcgtgatcca ccgggggcag ttgccccagg
     5881 gcttccaccc gttgcaggta ggccaggtaa ttgtttttca gtcctgtttc ttcgttgtct
     5941 atcagttcct gcacttcctg tttgcgcagc agctgtacca gcgcatccac acaaacagcc
     6001 accagtgtat c
//
LOCUS       DAOSOF010000118         5923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_13582_length_5923_cov_4.759543, whole genome shotgun sequence.
ACCESSION   DAOSOF010000118 DAOSOF010000000
VERSION     DAOSOF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13582_length_5923_cov_4.759543"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLM68_06955"
     CDS             <1..408
                     /locus_tag="PLM68_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55133.1"
                     /translation="AEIKKHGKVASVAVFANPEESRKLVRQDWPRFRNVDIFFPMIYH
                     KFYNWDDYQVLTATREGVQELKEAGNDGILCSGLFVGHVPQDRIMEFMLYTRTGGSAG
                     ICFFSIDNIDRTYGYWARLKSDIAQFKEDGESR"
     gene            441..1196
                     /locus_tag="PLM68_06960"
     CDS             441..1196
                     /locus_tag="PLM68_06960"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOT55134.1"
                     /translation="MKAEDVQKELRAHASAERKKAVEWYFKTGKGQYGEGDIFIGVST
                     PDARRISLKYRSLPFEELQKLIASPIHEERSCALTILVQQYEKGGERQASSATAAGNG
                     PAGGERQAVLDFYLRNRQYVNNWDLVDCSASKILGRAIHDGLQEAAILDRLVVSDILW
                     ERRIAIISTLYFIQKGQLEHTFRLSERLLGDDQDLMHKAVGWMLREAWKKDATATEAF
                     LIRHYPQVPRMALRYAIERMEEGKRKQFLKGVF"
     gene            1321..2073
                     /locus_tag="PLM68_06965"
     CDS             1321..2073
                     /locus_tag="PLM68_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT55135.1"
                     /translation="MKQPDLGKKITELRLAKGLTQGELAQKCNLSLRTIQRIESAEVT
                     PRSYTVKLIFQCLDYQIYDSFGKFTYRLDRLAYRIRIGLGQCYKYVLDLFNLKTHTMR
                     KITILSLPCIAIIFLFLFLGTESKAQSHEKIIARIATQNNKFTKWFNTGQIDSLGSVY
                     LENACMIPSNYREIHGREYIKGYYSFLYATGFRFTENTSSALVISDSILVDRGVWKAG
                     QMTGTYLTQWRLCEDGEWYIENEMTNTDLVEE"
     gene            complement(2133..2720)
                     /locus_tag="PLM68_06970"
     CDS             complement(2133..2720)
                     /locus_tag="PLM68_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55136.1"
                     /translation="MKKITVIMLALLLTAFACNNNPKGNEPKVSFGIYETVMLRDLPV
                     SAVAGQPGLCNLIPGTDSLSPIVAYVPIDSTFTIDTPVEQEVKFLRTTYPVDPEKQYY
                     GIVAVREQPTMTNIDLKTTKPDKDAVKITFTKEGSKKWAEMTRINIGKTVAISIDDQV
                     YTLPKIMAEIGVGRAMIIVENEETAVRLSAALNGE"
     gene            2928..4235
                     /locus_tag="PLM68_06975"
     CDS             2928..4235
                     /locus_tag="PLM68_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT55137.1"
                     /translation="MPDDMTVQEKIYGLSKLWQEVNYNFVYLEKVDRQAWDQAYLTAI
                     QTVMNTANDYEYYRELQKFCALLKDGHTNVYFPERIDTLIYNSDFGDYRLFLENVDGE
                     AIIARVNQSKKNVLPVGSRILEVNRIPVWDYIDRHVAPYIGSSTEYVLEDLAVMRLLE
                     GPVGTTYTIKIMKPDGSTATFDLKHERSNEKEVYPPLEPEKLMDFKMYDQIAYVALNS
                     FRSNSLDSLFTGILPQLSRAKGLIIDLRLNSGGKTNVGVNILEYITSDSLFYGSLSMS
                     REHIPTLKAWGSSVQPQDTTGNAWRKKCLLYYQDKMYYTFDYSPWVCGPKSQRFVKPT
                     VVLTGHLTASAAEDFLVFADNQEHIIRMGEKTYGSTGQPFVFDLPGGGFAIVCTKKDV
                     FKDGREFVGYGIVPHITVKRTLQDYYDQRDAVLEKALEYLNEK"
     gene            4255..4617
                     /locus_tag="PLM68_06980"
     CDS             4255..4617
                     /locus_tag="PLM68_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT55138.1"
                     /translation="MSQLHFKYKFWIEDQDGMSILGDGKYRLLKVIEETGSLKHAVAK
                     LNLSYRKTWDKLRNIERKLGYPILETQQGGPSGGTTTLTENARKLMEAFEKLHRECDP
                     YFKETIQKISGTDSATTE"
     gene            4731..>5923
                     /locus_tag="PLM68_06985"
     CDS             4731..>5923
                     /locus_tag="PLM68_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT55139.1"
                     /translation="MNNAVYNFPLPENEPILNYLKGSPERVLLENELKRQSQTVLEIP
                     LIIGGKEIRTGNFGEIRMPHDHNKVIARYHKAGKAEVELAIKAALEARKSWSDLAWTI
                     RASILLKAAELLSTRYRSVISAANMLGQSKNIYQSEIDAVCETIDFLKYNISFTGRIY
                     EQQPKSTFNQLNKMEYRALEGFVLAVSPFNFTSIASNLNMAPVMMGNTTIWKPASTAI
                     LSNYYLMKVFMEAGLPAGVINFLPGQGSVIGNTCLASKDLAGIHFTGSNSTFNGLWTQ
                     VSSNLSLYKSYPRLVGETGGKNFVFVHPSANVNDVCINTIRGAFEYQGQKCSAASRMY
                     VPRSLWPELKKCLGELAEEIKMGDVMNPENFMNAVIDEKAFDNIKSYIDYAKRSPDAS
                     IIAGG"
BASE COUNT         1740 a         1307 c         1405 g         1471 t
ORIGIN      
        1 gccgagatta aaaaacacgg taaagtggct tcggtggccg tttttgccaa ccccgaagaa
       61 tcccgcaagc tggtgcgtca ggactggccc cgctttagga acgtagacat tttctttccc
      121 atgatctacc ataaattcta taattgggac gattaccagg tcctaacggc aacccgggaa
      181 ggggtgcagg aactgaagga ggccggcaac gacggaatcc tttgttccgg cctgttcgtg
      241 ggacacgtcc cgcaagacag gatcatggaa ttcatgctct acacccgcac cggaggctcg
      301 gccggtattt gctttttttc aattgataac attgaccgca cctacggcta ctgggcacgc
      361 ctgaaatccg atatcgccca atttaaagaa gacggggaat ccaggtgatt tccagagtgg
      421 taattcaaca taaggttcat atgaaagcag aagacgttca aaaggaactc agggctcatg
      481 cctcggcaga acgcaaaaaa gcggtggaat ggtatttcaa gaccggaaaa gggcagtacg
      541 gcgagggaga cattttcata ggcgtgtcca cccccgatgc ccgcaggatc tccctcaaat
      601 accgaagcct tccctttgaa gaactgcaga aactcattgc ctcgcccatc cacgaagaac
      661 gcagctgcgc actgaccata ctggtgcaac agtatgaaaa gggtggggag cgtcaggcat
      721 cctctgccac agcagccggg aatggacctg caggtgggga gcgtcaggcc gttctggact
      781 tttacctgcg caacaggcag tacgtgaaca actgggacct ggtggactgc tcggcatcca
      841 agatcctggg acgggccatt cacgatggcc tccaggaagc tgcgatcctt gaccggctgg
      901 tggtttcgga cattctatgg gaacgccgca tagccatcat ttccaccctg tatttcattc
      961 agaaaggaca actggaacat accttccggc tttctgaacg gcttttgggt gatgaccagg
     1021 acctgatgca caaagccgta ggctggatgc tacgcgaagc ctggaaaaaa gatgccacag
     1081 ccacagaggc tttcctcata aggcactacc cgcaagttcc ccgcatggca ctccgctacg
     1141 ccatagaaag gatggaagag ggcaagagaa agcaattcct gaaaggcgtt ttttgagagt
     1201 ccgtaaagtg cagggggatt tacagttata aagtgacgca taaatgacgt attaaatacc
     1261 ggataaatat ttgaatagtt tttaagttac ctataatctt gtcacgaatt cgaatgaatc
     1321 atgaaacaac ctgacttggg taaaaagatc actgaattac gtttggctaa gggtttgacc
     1381 cagggcgaac ttgcacaaaa atgcaatttg agcctcagaa cgatccaacg tatcgaatcg
     1441 gctgaggtca cccccaggag ctataccgtg aagctgatct ttcagtgtct ggattaccag
     1501 atctacgatt catttggcaa attcacctat aggctggaca ggctggctta caggatacga
     1561 atagggctcg ggcaatgtta caaatatgta ctggatttat ttaacttaaa aacacacacc
     1621 atgagaaaga tcacaattct atccttaccc tgtattgcaa taatttttct ttttctattc
     1681 ttagggactg agtcaaaagc tcagagccat gaaaagatca tcgccaggat agctacacag
     1741 aataacaaat tcacaaaatg gttcaatacc ggccagattg actctcttgg atcggtatac
     1801 ctggaaaacg catgtatgat cccctccaat taccgggaga tccatggccg ggaatacata
     1861 aaaggttact acagcttttt gtatgccacc ggatttcgtt tcactgaaaa tacttccagt
     1921 gcactggtca tatcagattc catattggta gacaggggag tctggaaagc cggccagatg
     1981 acaggaacgt atcttaccca gtggcgtctc tgtgaggacg gtgaatggta catagaaaat
     2041 gagatgacaa atacggatct tgttgaagaa tgagaacctg aactccggca aatttcttgc
     2101 cgaatcgccg gtcgcataat caaaaatatc tatcactcac cattcagagc ggccgatagc
     2161 cttacagccg tttcttcatt ttccacgatg atcatggctc tgcctacacc gatctcggcc
     2221 atgatttttg gcagggtata gacctggtca tcaatggaaa tggcgactgt tttcccaatg
     2281 ttgatccgtg tcatttctgc ccatttcttt gagccttcct tagtgaatgt gatcttcact
     2341 gcgtctttgt cgggtttagt ggttttcaaa tcaatgttgg tcatggttgg ctgttccctg
     2401 accgccacta tgccataata ttgtttctca ggatcaaccg ggtaggtggt gcgcaaaaat
     2461 ttcacctctt gttccacagg agtgtcaatg gtaaaggtcg agtcaatggg cacataggca
     2521 acaatggggg aaagagaatc ggtacccggg atcagattgc agagccccgg ctgcccggca
     2581 accgcggaga ccggaagatc cctaagcatt acagtttcat agattccgaa ggatactttt
     2641 ggctcattcc ctttgggatt gttgttgcag gcaaatgctg tcagcaacaa ggcaagcatg
     2701 ataacagtga tttttttcat aataatagca gaattattaa accaggtttg taatacaaac
     2761 gtggtaaaaa taatggtatg ttgtaacgtt attttcagcg ttgcgtcaat agactgttaa
     2821 aaaaaaagta gatgattatg agaactttaa aaattttgat gtgtttttca attgtatgct
     2881 gctccctaaa tttccttacg gctcagcaga tcacaaccaa tcacaaaatg ccggatgata
     2941 tgacggtaca ggaaaaaatt tacgggctgt caaaattatg gcaggaagtg aactacaact
     3001 ttgtttacct ggaaaaggtc gacaggcaag cctgggatca ggcttaccta actgccattc
     3061 agacagtaat gaatacggcc aatgactatg agtattacag ggagctgcaa aaattctgtg
     3121 ccctgctaaa ggacggacat acgaatgtat atttcccgga aagaattgat acacttatat
     3181 ataattccga ttttggtgat tacaggctgt tcctggaaaa cgttgacggg gaagcgatca
     3241 ttgcaagagt gaatcaaagt aagaaaaatg tattgccggt cggctcccgg atcctggaag
     3301 ttaaccggat cccggtatgg gactatattg atcggcatgt ggctccttat atcgggtcat
     3361 caacagaata tgttttggaa gacttggccg ttatgaggtt gctggaaggg ccggttggaa
     3421 ctacctatac tattaagatc atgaaacccg acggaagtac cgctaccttt gatctgaagc
     3481 atgaaagatc caatgaaaaa gaggtatatc cacccctgga gccagaaaaa ctgatggatt
     3541 tcaaaatgta tgatcaaatc gcctatgtgg ctcttaactc gttcagaagc aacagcctgg
     3601 attccctctt taccggaata ttgccccaac tgagccgtgc caaaggattg atcatagatc
     3661 ttcgtctgaa tagcggcgga aagaccaatg taggcgttaa cattttggaa tatatcacca
     3721 gtgactcact gttttacggt tctttatcca tgagcaggga acacataccc acattaaagg
     3781 cctggggaag ctctgtacag ccacaggaca caacgggaaa tgcctggaga aaaaaatgcc
     3841 tgctttatta ccaggataaa atgtattata ctttcgatta ttctccctgg gtttgcgggc
     3901 ccaaatccca acgatttgtc aagcccacag ttgtcctcac cggacacctg acagcctcgg
     3961 ccgcagaaga tttcctggtt tttgcagaca accaggagca tataataagg atgggggaga
     4021 aaacttacgg aagtaccgga cagccgtttg tatttgatct gcctggcggg ggatttgcca
     4081 tagtatgcac aaaaaaggat gttttcaaag acggcaggga atttgtaggt tacggcatag
     4141 taccgcatat tacagtaaaa agaacactgc aggattatta cgatcagagg gatgcagtcc
     4201 tggagaaggc gctggaatat ttaaacgaaa agtagaagat acaatcagat ttttatgagc
     4261 caattgcatt ttaaatacaa gttctggatc gaggatcagg atgggatgag tattctgggt
     4321 gatggcaaat accggttact aaaggttatt gaagaaacag gatcgttaaa acatgctgtt
     4381 gccaaactaa accttagtta cagaaagacc tgggacaagc ttcgcaatat tgagagaaaa
     4441 ttgggttatc ccattctgga aacgcagcag ggagggcctt caggaggaac taccacgtta
     4501 acagagaatg caagaaagct tatggaagcc ttcgaaaagt tgcatcggga gtgtgaccct
     4561 tacttcaagg aaactatcca aaagatttca ggcacggaca gcgcaacaac tgagtaaacc
     4621 aaggattcca ttaacaattg ccatgcatat gttcacagat ggttgggaag ttacaagcga
     4681 attgcatatt tttgcaactc gcatcatgta aattctaaaa taactatacc atgaacaacg
     4741 ctgtctacaa tttccctttg cctgagaatg aacccatact gaactatctg aaaggaagtc
     4801 ctgaacgcgt actgttggaa aatgagctga aaagacaatc ccagactgtc ctggaaatac
     4861 cgctgattat tggcggaaaa gaaataagaa cgggcaattt tggagagatc cggatgccgc
     4921 acgatcacaa taaagtgatt gccaggtatc ataaagccgg aaaggcagaa gtggaattgg
     4981 ccataaaggc agcactggaa gcccggaaat cctggtcaga tctggcctgg accatcaggg
     5041 cttccatatt gcttaaggcc gcagaactcc tttccacacg ctaccgttct gtgatcagtg
     5101 ccgccaatat gctcggacaa agcaagaata tctatcagtc agagatagat gctgtttgtg
     5161 aaaccattga cttcctgaaa tacaatatca gttttacggg acggatttat gaacaacagc
     5221 ccaagtccac ttttaatcaa ttgaataaaa tggagtacag ggccctggaa ggtttcgtgc
     5281 tggctgttag cccgtttaac tttacttcta ttgcctcaaa cctgaacatg gccccggtca
     5341 tgatgggcaa tacaaccatc tggaagcccg cttccacggc catactgtcc aactactatt
     5401 tgatgaaagt ctttatggaa gccggattgc ccgccggtgt gattaacttt ttacccggac
     5461 agggatccgt tattggaaac acgtgcctgg cttcaaaaga ccttgcaggg attcatttca
     5521 ccggatccaa tagtactttc aacggtttat ggacgcaggt aagttccaac ctctccctct
     5581 acaaatccta tccccgcctg gttggggaaa ccggaggaaa aaactttgtt tttgtgcacc
     5641 cctcggcaaa tgtaaacgat gtctgtataa ataccatccg gggtgctttt gaataccagg
     5701 gacagaaatg cagtgcggct tccaggatgt acgtaccccg ttctttatgg cctgagctga
     5761 agaaatgtct tggcgaactt gccgaagaaa ttaagatggg agacgttatg aatccggaaa
     5821 atttcatgaa cgccgtgata gacgaaaagg cctttgacaa tataaaatct tacatagatt
     5881 acgccaaaag atcacccgat gcctcaatca tagccggcgg cca
//
LOCUS       DAOSOF010000119         5711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14270_length_5711_cov_4.102723, whole genome shotgun sequence.
ACCESSION   DAOSOF010000119 DAOSOF010000000
VERSION     DAOSOF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14270_length_5711_cov_4.102723"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLM68_06990"
     CDS             <1..521
                     /locus_tag="PLM68_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOT55140.1"
                     /translation="FDRTEAQKRIHFASAMTLLGFKDGDNATTGKGYLDIVDFIVRGC
                     TDVRSNLEELFRRVAFNIGVGNSDDHFRNHGFLLTPKGWTLSPAYDMNPTLDETQSLL
                     LTQTTNEANPQLLLDACSEYMLDRKTASGIMAEVGYALSDWEKTARILNIPGQEIEMF
                     RWRFEKMLTLFK"
     gene            528..3032
                     /locus_tag="PLM68_06995"
     CDS             528..3032
                     /locus_tag="PLM68_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT55141.1"
                     /translation="MKKIYTLFLAMVVCGAVVNIPVAYAQINPVIPRDPAIEKKIDKI
                     ISKMTLVEKVGQMTQLEINVLGMDMRAEMMKLMMMPQEQLAALISEHGLESEFDAAVM
                     TNPEKRMEMGFEYYKLYQKITVTKGFQFDPAKMDSVFVKYKVGSILNTPFTTAQTSEE
                     WNRLIKIVQDKSLEAIGIPCLYGLDQIHGSTYVADGTLFPQGVNMAATFNRELARCTG
                     EVTAYETRAAGIPWSFNPVLDMGRQPAWPRQWEGYGEDPYLASEIGREVVMGMQGTDP
                     NHIGRENISACLKHYMGYGVPFNGLDRTPAIINEQDMREKQFAPFLEALRNGALSIMT
                     NSSTINGVNGVANPVLLTQWVKQDLSWDGMIVTDWADITSLYERDRIASSYKEAVKMA
                     INAGIDMAMVPSSWQFCIDLKELVEEGQVPMSRIDDAVRRILRLKFRLGLFDTPYTVR
                     KDYSRFGDPAFAALSLEAAGESMVLLKNEEATLPLPKGKKILVAGPNAHTMRGLNGGW
                     SYTWQGSGIERFTEPLNTILEAMKNEFGPDNILYEPGVLYNESGYWTMENKPEIEKAV
                     AAVAGADYIVVCIGENSYCETTGNINDLHISKNQADLVKALAATGKPVIMILNQGRPR
                     VVAELVPLAKSIVNVMLPGNYGGDALASLLSGKVNFSGRLSYTYPSCCNGYTTYDYKV
                     CEVRSTIEGAYDYFANTNIQWPFGYGLSYTTFEYSNLKADKTTFTAVDELVFTVDVKN
                     TGKVEGKESVLLYSSDLYASLMPDNRRLRAFDKISLRPGEQKTVTLKIRASDLAFVGI
                     DGRWVLEEGDFKIGAGNQSLMIRCTQTHVWNTPNRP"
     gene            3077..4051
                     /locus_tag="PLM68_07000"
     CDS             3077..4051
                     /locus_tag="PLM68_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT55142.1"
                     /translation="MMRLVFLDAATMGGDIDLGPLRQFGELTVYDNTRPEEIIARIEK
                     ADIVLLNKVKLGKAQIQAARNLKLICVAATGLDPVDLDYAAEKGIPVKNVAGYSTDSV
                     AQTAFLHILCCVQQGPYFNEYAHSEAYPASGLPTHFGGRYFHELSGKRLGIIGLGAIG
                     RKVAAIASAFGMEVVYFSTSGQERAGGLYRRLDLDELLRTSDLVTIHAPLNDKTRGLI
                     GKAGLDKMKASAYLFNLGRGAIVDECALVDALNNNRLAGAGMDVFSTEPLPADHCYHR
                     VKDKSKLFLTPHIAWASIEARTRLVAMMADNISQYIASRSGDRSRAGK"
     gene            complement(3999..4292)
                     /locus_tag="PLM68_07005"
     CDS             complement(3999..4292)
                     /locus_tag="PLM68_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT55143.1"
                     /translation="MGISKQLIAVQRFDKEGEPFEFYVNPIIENYSQETQWGWEGCLS
                     VPGERDTVLRSTQVVITYRDQDTWEMARDTVSGFTAVIFQHEIDHLNGKLYID"
     gene            complement(4211..4570)
                     /locus_tag="PLM68_07010"
     CDS             complement(4211..4570)
                     /locus_tag="PLM68_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55144.1"
                     /translation="MKKCLISLLLLLAVACSNFSSIEKYRIGKPGESEMIMPLFTNQI
                     PEELQFLRLKAGPLSRRDMKSNLYSTLKSRMMATVLDTANTGVGLLPRRWVSASNLLP
                     SNVLIRKENLLSFMSIP"
     gene            complement(4595..5419)
                     /locus_tag="PLM68_07015"
     CDS             complement(4595..5419)
                     /locus_tag="PLM68_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOT55145.1"
                     /translation="MLILFQWFNPAAWLLKQSMQQVHEFQADDAVLKAGVNAKQYQLL
                     LIKKAVGTRRYSMVNSFNHSKLKKRITMMWKKKSSKWAFAKCIYALPLAFIAVTAFAT
                     PEISGTLSGISSVKDIKISLPQDIKPQALPVTAQVPPPPPAPAAAIPAKQDSVFPLAL
                     LDTKPLFANGKDENEFTKWVLNNMKYPEEAKKKGIQGRVIASFELSETGQVVNAKILR
                     GVDPELDNEVLRVLMSSPVWERPGQTKGKTVRVRYNFPVNFQLPKKEESKAPEGTK"
     gene            complement(5476..>5711)
                     /locus_tag="PLM68_07020"
     CDS             complement(5476..>5711)
                     /locus_tag="PLM68_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55146.1"
                     /translation="CFLFFVISYIKLTAILWKNKGNGKGEVFGNLLDECKKLCRVRGR
                     VRLLVHTQETAPFSWMRYVVVSEQDMIEDGKDI"
BASE COUNT         1462 a         1409 c         1489 g         1351 t
ORIGIN      
        1 gttttgaccg gaccgaagcg caaaagcgca ttcactttgc ctcggccatg accttgcttg
       61 gctttaaaga tggagacaat gccactacgg gaaaaggtta cctggatatt gtggatttta
      121 ttgtgcgggg atgcacagat gtccgttcta atttagaaga actcttcagg cgggtggcat
      181 ttaatattgg cgtgggaaat tcagacgatc attttcggaa ccatggattt ttacttaccc
      241 caaaagggtg gacgctctcc cctgcgtatg atatgaatcc gacactagat gaaacccaaa
      301 gcctgctcct gacccagacc acgaatgaag caaatcccca actgttgctg gatgcctgtt
      361 ctgagtatat gctggatcga aagacggcat caggtattat ggccgaggtt ggttatgcgc
      421 tttcggattg ggagaaaacg gccaggatcc tgaacatacc tggacaggaa attgaaatgt
      481 tccgttggcg ctttgaaaaa atgcttactt tatttaaata aataatcatg aagaaaattt
      541 acaccctttt ccttgctatg gttgtatgcg gcgctgtcgt gaatataccc gtcgcatatg
      601 cccagatcaa cccggtgata ccgcgcgacc cggccattga aaaaaagatt gacaagatta
      661 tctccaaaat gaccctggtg gaaaaagtgg gtcagatgac ccagcttgag atcaacgtac
      721 tgggcatgga catgagggca gaaatgatga aactgatgat gatgccacag gagcagctgg
      781 ccgcactaat cagtgagcac gggctggaaa gcgagtttga cgccgctgtg atgaccaacc
      841 cggaaaaacg gatggagatg ggatttgaat attataaact ctatcagaaa ataacggtta
      901 caaaaggatt ccagtttgat ccggcaaaaa tggactcggt gtttgtcaaa tacaaagtgg
      961 gctccatcct gaacactccc tttacaacgg cgcagacttc ggaagaatgg aaccggctca
     1021 taaagatcgt tcaggataaa tccctggaag cgatagggat cccgtgcctg tacggcctgg
     1081 accagatcca tgggtcaacc tatgtagccg atggcaccct gtttccacag ggagtgaaca
     1141 tggcagctac ctttaacagg gaactggccc gctgtaccgg tgaggtcacc gcctatgaaa
     1201 cacgggcggc agggataccc tggtcattca acccggtcct ggatatgggc cgccaaccgg
     1261 catggccccg gcagtgggaa ggctacgggg aagatcctta cctggcctcg gaaatcgggc
     1321 gggaagtggt gatgggcatg cagggaacgg atcccaacca tattggcagg gaaaacatct
     1381 cggcctgcct gaaacactat atgggctacg gagtgccttt caacggcctg gaccgcaccc
     1441 cggcaatcat caacgaacag gatatgcgcg aaaagcagtt tgcccctttt ctggaagccc
     1501 tgcgcaacgg tgcgctgtcc atcatgacca attcttccac catcaacggc gtgaacggtg
     1561 tggccaatcc cgttctgctt acccaatggg ttaagcaaga cctgagctgg gacgggatga
     1621 tcgttacaga ctgggccgat attaccagtt tgtatgaacg cgacaggatt gcctcgtcgt
     1681 acaaggaagc cgtcaaaatg gccatcaacg cgggcattga catggccatg gtgccttcca
     1741 gctggcagtt ttgcatagac ctgaaagaac tggtggaaga aggccaggtg cccatgagcc
     1801 gcatagacga tgccgtacga cgcatcctgc gtctgaaatt ccgcctggga ttgtttgata
     1861 ctccttatac cgtgcggaaa gattattccc ggtttggtga tcccgccttt gctgcactat
     1921 cgttggaagc agccggggag tccatggtac tgctgaagaa tgaagaagcg accctcccgt
     1981 tgcccaaagg taaaaagatc ctggtcgccg gacccaatgc ccataccatg cgcggcctga
     2041 atggcggctg gagctatacc tggcagggca gcggcattga acgctttaca gaaccattaa
     2101 acaccatcct ggaagccatg aaaaacgaat tcggccctga taacattctg tatgagcccg
     2161 gtgtgcttta caacgagagc gggtactgga ccatggaaaa caaaccggaa attgaaaaag
     2221 cagtagccgc tgtagcaggg gcagattaca ttgtggtttg cattggagaa aattcttatt
     2281 gcgaaaccac aggcaatatt aacgacttgc atatctcaaa gaaccaggca gacctggtaa
     2341 aggctttggc tgccaccgga aaacccgtca tcatgatcct gaaccagggc cgtcccaggg
     2401 ttgttgccga gctggtgcca ctggcaaaaa gcatagtgaa tgtaatgctt ccgggcaatt
     2461 acggcgggga tgccctggcc agtctgcttt ccggaaaagt gaacttcagc ggcaggctct
     2521 cctataccta tcccagttgc tgcaacgggt atacaactta cgattataaa gtatgtgaag
     2581 tgcgttccac catagaaggc gcatacgatt actttgccaa cacgaacatt cagtggcctt
     2641 tcgggtacgg actcagttac acgacctttg aatattccaa cctgaaagca gacaagacaa
     2701 ccttcacggc agtagatgag ctggtcttta ccgtggatgt gaaaaatacc ggcaaggtgg
     2761 aaggaaaaga aagtgtacta ctttattcct cagatttgta tgcctctctg atgccggata
     2821 accggcgcct cagggcattt gataagatat ccctcagacc cggagaacaa aagaccgtta
     2881 ccctgaagat cagggcaagc gatcttgcct ttgtgggcat tgatggcaga tgggtgctgg
     2941 aagaaggcga tttcaagatt ggagctggta accagagcct gatgatccgc tgtacgcaga
     3001 cccatgtgtg gaacacaccc aaccgcccgt aaatcatcag aaacagataa tcccggggat
     3061 tccctaactt tgccgtatga tgagactcgt ttttttagat gcagccacca tgggtggtga
     3121 tattgacctg ggccctctca gacaattcgg ggagctgacg gtatacgaca atacgcggcc
     3181 ggaagaaatc attgcccgta ttgaaaaggc agatattgta ctactgaata aggtaaaact
     3241 gggcaaagcg cagatacagg ctgccaggaa cctgaagctg atctgtgttg cagcaacggg
     3301 cctggacccc gtagacctgg actacgcggc ggagaaaggc atcccggtaa aaaacgttgc
     3361 gggctactcc acagacagtg tggcacagac cgccttcctg catatcctat gttgtgtcca
     3421 gcagggcccc tatttcaacg aatacgccca ctcggaagct tacccggcca gcggcctgcc
     3481 tacccacttc ggggggcggt atttccatga gctaagcgga aaacggctgg gtatcatcgg
     3541 gttgggggcc ataggaagga aagtggcggc cattgcctcg gctttcggaa tggaagtggt
     3601 ttatttttcc accagcggcc aggaacgcgc cggcgggctg tacaggcgcc tggacctgga
     3661 tgaactgctg cggacaagcg acctggtgac catccacgct ccgctcaatg acaagacccg
     3721 cggtttgata gggaaagccg ggctggataa gatgaaggcc tcggcctatc ttttcaacct
     3781 ggggcgggga gccatcgtgg atgaatgtgc actggtcgat gcgctgaaca acaaccggct
     3841 ggccggtgca ggaatggatg ttttcagtac cgagccgctt ccggccgatc attgctacca
     3901 tcgggtaaag gacaagtcaa aactgttcct tacgccgcac atagcctggg cctcgataga
     3961 agcgcgcacg cgcctggttg ccatgatggc agataacatc agtcaatata tagcttcccg
     4021 ttcaggtgat cgatctcgtg ctggaaaata accgctgtaa agcctgagac cgtatcccgg
     4081 gccatctccc aggtatcctg gtcccggtaa gttatgacca cctgggtaga acgcagcacg
     4141 gtatctcttt ctccgggaac cgaaaggcaa ccttcccatc cccattgggt ctcctggctg
     4201 tagttttcga ttatgggatt gacataaaac tcaaaaggtt ctccttcctt atcaaaacgt
     4261 tggacggcaa taagttgctt gctgataccc acctgcgggg cagcaatccc accccggtgt
     4321 tggccgtatc caggaccgtg gccatcatcc tggacttaag ggtggaatac aggtttgatt
     4381 tcatgtctct ccggctaagc ggacctgctt tcagacgcag gaactgcagc tcttccggga
     4441 tctggttggt gaatagaggc atgatcattt cagactcacc gggctttcca atgcggtatt
     4501 tctctataga agaaaaattg ctgcaagcga cagccagcag caataacagt gatatgaggc
     4561 attttttcat gctcaaagat aggaatcttt gagcttactt tgtcccttcc ggagccttag
     4621 attcttcttt cttgggtagt tgaaagttta cgggaaaatt atacctgacc cttactgttt
     4681 tccccttagt ctgtccggga cgttcccaaa cgggagaaga catcagaacg cgtaagactt
     4741 cgttgtccag ttcaggatct actcccctga gaattttagc attgaccacc tgtcctgttt
     4801 cagacagctc aaaactggca ataacccttc cctgaatccc tttcttcttg gcttcttccg
     4861 ggtatttcat gttatttaaa acccatttgg taaactcatt ctcgtctttg ccattggcaa
     4921 acaatggctt ggtgtctaac agggccaaag gaaacacaga atcctgctta gcgggaatgg
     4981 cggcggcggg tgcagggggc gggggcggta cctgtgcagt tacgggcagg gcctggggtt
     5041 tgatatcctg cggcaaagaa atttttatat ctttgacaga ggaaatcccg gacaacgtgc
     5101 cggagatttc aggcgttgca aaggccgtta cggctatgaa cgcgagaggc agagcgtata
     5161 tgcactttgc aaacgcccat ttgcttgatt tctttttcca catcatagtg attctttttt
     5221 taagtttgct gtgattaaag ctgttgacca tggagtagcg tcttgtgcca acagcttttt
     5281 ttattaataa taattggtac tgttttgcat ttacaccggc tttcaggaca gcgtcatcgg
     5341 cctggaattc atggacctgc tgcatggatt gcttcagaag ccaggcagcg ggattgaacc
     5401 attggaaaag aatgagcaag tctgtcagta tcaggtccca ggaatgtccc tgacgaacat
     5461 gggataattc gtggatcaga tgtctttccc gtcctcaatc atatcctgtt cagataccac
     5521 tacgtaacgc atccagctga acggggccgt ttcctgcgtg tgaaccagta gcctgaccct
     5581 ccctctgacc ctgcaaagtt ttttgcattc atccaacaga ttcccaaaca cctctccctt
     5641 tccgttgcct ttgtttttcc acaatatggc cgtcagtttt atatacgata tgacaaagaa
     5701 caggaaacaa a
//
LOCUS       DAOSOF010000120         5703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14297_length_5703_cov_5.418201, whole genome shotgun sequence.
ACCESSION   DAOSOF010000120 DAOSOF010000000
VERSION     DAOSOF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14297_length_5703_cov_5.418201"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLM68_07025"
     CDS             <1..782
                     /locus_tag="PLM68_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOT55147.1"
                     /translation="KIIEMNLRNSVFADCTADHFIALRYEDIIMAKIAIVTCNKIANT
                     LDMDYYNKLRRQAVRRHVPFLYETNVGAALPVICLIRQMRLSGDTPERVEACLSGTLN
                     YVFSRYDGQTPFHRIVEEASSLGYAEPDPMADLSGKDVMRKTLILARECGMDIEESQV
                     NTASFLPTQWPCEEHFRSLYLESQQKEGTLRYVSTIDNRGVKAGLKVVPREHPFSGLR
                     GTDVSVILYSRLYPNGLRVEGAGAGGRQTASGLINDILQCV"
     gene            779..1786
                     /locus_tag="PLM68_07030"
     CDS             779..1786
                     /locus_tag="PLM68_07030"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT55148.1"
                     /translation="MNRNVMVAGATGLVGRTMIKILEERNFPADHLVAVASERPEGRT
                     VSFRGKKIGVTTLPAALKEEPGIAFFSAGKDVSGTWAPLFVQAGWKVVDNSSCWRMSG
                     NVKLVVPEINGSLLSPDDRLVANPNCSTIQMVLALAPLHNRYRLQRIVACTYQAVTGS
                     GQKGVERILAERKAWVAGNPLPLSGSVYPCPIDLNVIPDIGALTPDGYTEEEIKMREE
                     TRKILGDAKIGVSATCVRVPVIASHCVAVNARFAEPFSLEDVCSLIKSFPGLVLNDFS
                     AGHGFAVPLEAEGTDTVYVGRIRRDPTEENTLELWTVADNLRKGAALNAIQIGELLLQ
                     F"
     gene            complement(1788..2351)
                     /locus_tag="PLM68_07035"
     CDS             complement(1788..2351)
                     /locus_tag="PLM68_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55149.1"
                     /translation="MLYEGTPEDPARLQLNAAENYARYHLLSLMEQLRRSSDPKPLRY
                     LVLGCTHYPYQLETLTLMLEHLRQFRQGDQYPYRDLIAPDVEIIDPALETARELYYTL
                     LKDNLLTYRLGQSNAQFYLSVSRKDPDNPQRLDSLGRFTYEYKYGRMAGLFAQDIDIV
                     PFSADNIDSLTIERFRSLRHTWPLLPF"
     gene            complement(2525..3151)
                     /locus_tag="PLM68_07040"
     CDS             complement(2525..3151)
                     /locus_tag="PLM68_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOT55150.1"
                     /translation="MMNIRKDLLPVLVLFLASCQGRPTHIPIIETALNDSNSVYYTDF
                     SAYPAVRNDLPIGIFDSGTGGLTVMEAIMASRLLDGENYIYFGDQANMPYGNYPSEGK
                     TDFLRELIMQDAFFLLGRKVKILVVACNTATAYGLEDIRAYLDESGSGIKVIGVINAG
                     VNATLDRIKPGEEVAVGVLATVGTVASGGYENTFRSLALSGVTVTISW"
     gene            complement(3148..3957)
                     /locus_tag="PLM68_07045"
     CDS             complement(3148..3957)
                     /locus_tag="PLM68_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOT55151.1"
                     /translation="MIHFDNHTHSHHSADSRMAPGRAAEKALQRSLGGICFSDHYDFD
                     APEGVRLFTFDVQRQQEEIEGLKEKFPSIVLLKGVEIGIQPISLGAIRDFMGKHAFDT
                     VIASMHFVRGTDPFHRTYYQGYDFKQAYGIYLEDILSCISVFPDFDILGHFDYVARYS
                     PYLEHEVSMKVFGDVLDPILKILAQNGKTLEINTKTYQKFQYGTPTLDKNVIRRFREL
                     GGEAVSLGSDAHSDEKIGEQFGIYTDILRQCGFRYGVYYRNRRPEYYKIDS"
     gene            complement(3986..5266)
                     /gene="eno"
                     /locus_tag="PLM68_07050"
     CDS             complement(3986..5266)
                     /gene="eno"
                     /locus_tag="PLM68_07050"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT55152.1"
                     /translation="MKIKKVEAIEILDSRGNPTVEVRMSLEDGTRATAMVPSGASTGE
                     REAVELRDGDKKRYNGKGVLKAVSNVNYNIAKAIEGKCFTSQRELDYLMIDLDGTPNK
                     GKLGANAILAVSMAYARARSISLGIPLYQYLGGSNAHLLPVPCMNVINGGAHADNTVD
                     FQEFMIAPHNAPSFRESIRMGAEVFHALKSVLKSKGLSTGVGDEGGFAPDLKTNEQAV
                     EMILEGIVKAGYKPGEDVSICLDPAASEMWEDGKYKFFKSTKQLVSSDEMVKIWESWV
                     RQYPIVSIEDGLAENDWQGWKNLTDTIGNKIELVGDDLFCTNKAILAEGIKKGLANSV
                     LIKLNQIGSVTETLETIELANRNSYNCFVSHRSGETSDSFIADLAVAVNAGHIKTGSG
                     CRSERIEKFNQLMRIEYELGKVSHFAGLKAFKNA"
     gene            complement(5378..5450)
                     /locus_tag="PLM68_07055"
     tRNA            complement(5378..5450)
                     /locus_tag="PLM68_07055"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5415..5417),aa:Phe,seq:gaa)
BASE COUNT         1424 a         1434 c         1348 g         1497 t
ORIGIN      
        1 acaaaatcat agaaatgaat ctgagaaaca gcgtgtttgc agactgtaca gctgatcatt
       61 tcattgccct gcgctacgaa gatattatta tggccaaaat agccatagtg acctgtaata
      121 agattgccaa caccctggat atggattatt ataacaaact tcgccgccag gcggtcagaa
      181 gacatgttcc tttcctctat gaaaccaacg tgggcgcagc gcttccggtg atttgcctga
      241 tccggcagat gcgcctcagc ggggacaccc ctgaacgggt ggaggcatgt ctttctggaa
      301 ccctgaacta tgttttcagc cgttatgacg gccagactcc attccaccgg attgtggaag
      361 aagcctcaag tcttggttat gccgagccgg atccaatggc agacctttcc ggaaaggatg
      421 taatgcgcaa aacattgatc ctggcacgcg aatgtggaat ggacatagaa gaaagccagg
      481 taaacaccgc ctcgtttctt cccacgcagt ggccctgtga agaacatttc aggtccctgt
      541 acctggaaag ccagcaaaag gaaggaaccc tgcgatacgt ttccacaata gacaaccggg
      601 gcgttaaggc cggcctgaaa gtggtgcccc gtgaacatcc cttttcagga ctccgcggga
      661 cagacgtgtc ggttatcctt tacagccggt tatatcccaa cggattgcgc gtggaaggag
      721 cgggagccgg cggccggcag acagcttccg gattgatcaa cgatattctg caatgcgtat
      781 gaacagaaat gtaatggttg caggagcaac tggactggtg ggcaggacta tgatcaaaat
      841 actggaagaa aggaactttc cggcagatca cctggttgcc gtggcatcgg aacgccctga
      901 aggacgcacg gtttctttcc ggggaaagaa aatcggggtg accaccctgc ctgctgcact
      961 gaaagaggaa ccgggcatag ccttcttttc cgcagggaaa gacgtttccg gaacctgggc
     1021 tccgcttttt gtacaagccg gctggaaggt ggtagacaat tcttcctgct ggaggatgtc
     1081 cgggaacgtg aaactggtag tccccgaaat caatggtagt ttgctcagcc ccgatgaccg
     1141 gctggttgcc aatcccaatt gctctaccat tcagatggtc ctggcgctgg cacccctgca
     1201 caacaggtac cggctgcaaa ggattgtggc ctgcacatac caggcggtga ccggctcggg
     1261 acagaaaggc gtggaaagga tactggcaga aagaaaggcc tgggtagcag gaaatccgct
     1321 gcccctgtca gggagcgttt acccctgccc catagacctg aatgtgatac cggatatcgg
     1381 cgccctgacg cccgatggat acacagaaga agaaataaaa atgcgcgaag aaacccgaaa
     1441 aatattgggt gatgcgaaaa tcggggtttc tgccacttgc gtgcgcgtac cggtcatagc
     1501 gagtcactgt gtggcggtca atgcccggtt cgctgaacct ttttctttgg aggatgtctg
     1561 ttcgcttata aaaagtttcc ccggccttgt gttgaatgat ttttcagccg gtcatggttt
     1621 tgccgtgccc ctggaagccg aaggtacaga tactgtctat gtaggcagga tccggagaga
     1681 tcctacggaa gaaaacacat tggaattatg gaccgtggcc gacaaccttc gaaagggggc
     1741 tgcactgaac gccatacaga tcggtgaact gctgttgcag ttctgaatca gaacggaagc
     1801 agcggccagg tatgcctgag cgaacggaaa cgttctatgg tcagactatc tatattgtct
     1861 gcagaaaaag gcacaatgtc gatatcttgt gcaaacaggc ctgccatacg gccgtattta
     1921 tattcatagg taaaacgccc cagtgaatcg agtctttgtg ggttatcagg atcctttctt
     1981 gaaaccgata aataaaattg cgcattcgat tgtccaagcc tgtaagtgag cagattgtcc
     2041 ttcaacagcg tgtaatacag ctcgcgtgcc gtttccagcg ccggatctat gatttccaca
     2101 tccggtgcta tcaggtcccg gtacggatac tggtcaccct gcctgaattg gcgcaaatgc
     2161 tccagcatca gtgttaaagt ttccagctgg taaggataat gggtacatcc caggaccagg
     2221 taacgcaatg gctttgggtc actgcttcgg cgcagttgtt ccataaggct caacaggtgg
     2281 taccgtgcgt aattctccgc tgcgttcagt tgcaggcggg ccggatcttc aggtgtgcct
     2341 tcatataaca ttttattgga tgaaaaatca aagccatagg ctggcagcag atcaagttct
     2401 atcttgtatt gtgggtgatc cggcgacggt ccccggtatc catcccttgg accggatgca
     2461 tctgtgcaga taaaatccct ttccccgtca acggcttctg caaaacccag agatccatgg
     2521 ctaactacca ggatattgtc accgtaaccc cgctcagggc aagggaccgg aaagtgtttt
     2581 catacccacc cgaagccacg gttcccaccg ttgccaaaac gcctacagcc acctcctcac
     2641 cgggctttat cctgtccagt gtggcattga ccccggcatt gatcacgcca atgaccttga
     2701 tccctgaacc gctctcatca aggtacgctc tgatatcctc caggccatat gcagtagctg
     2761 tattgcaggc cactaccagt attttaactt tccgtccaag caggaaaaag gcatcctgca
     2821 tgatgagttc cctcagaaag tctgtctttc cctctgatgg ataattgcca taaggcatat
     2881 tggcctggtc cccgaaataa atataattct ctccatccag caggcggctg gccataatgg
     2941 cctccatcac ggtcagaccc cctgttcccg aatcaaaaat acctatcggc agatcgttac
     3001 ggacggcagg ataagcagaa aagtctgtgt agtatacaga atttgaatca ttaagagctg
     3061 tttcaattat gggtatgtgt gtcggcctgc cctggcatga agccaggaaa agtaccagta
     3121 ccggcaaaag atcttttctg atattcatca tgaatcaatt ttataatatt ccggtctcct
     3181 gttcctgtaa tacaccccgt aacggaatcc gcactgccgg agtatatcgg tataaattcc
     3241 gaattgttcc ccgatctttt catcactatg ggcgtccgat cccaggctta cggcttcacc
     3301 gcccagctcc ctgaatcgcc tgatgacatt tttatccagg gtcggggttc catattggaa
     3361 tttctggtac gtttttgtgt tgatttctaa tgtttttcca ttttgggcca ggatcttcaa
     3421 tatgggatcc agcacatcgc caaagacctt catggacact tcgtgttcca ggtatggaga
     3481 ataccgggcc acgtaatcaa agtggcccag tatgtcaaaa tcgggaaaaa cactgataca
     3541 agacaaaatg tcttccaggt atataccgta cgcctgtttg aaatcgtatc cctggtaata
     3601 agtacggtgg aaaggatccg ttccccggac aaagtgcatg gaggctatga ccgtgtcaaa
     3661 ggcatgtttg cccatgaaat cccttatggc gccgagactg atgggttgta tcccgatttc
     3721 cactcctttg agcagaacaa ttgaaggaaa tttttctttc aacccttcga tttcctcctg
     3781 ctgccgctgc acatcaaatg tgaacaacct cactccttca ggagcgtcaa aatcgtaatg
     3841 atccgagaaa catatccctc ccaatgacct ttgtagggcc ttttcagccg ccctgcccgg
     3901 agccatcctg ctgtcggcag aatgatggga gtgcgtatga ttgtcaaaat gaatcatccc
     3961 tccaggcttt tggaacacta acttttcagg cgtttttaaa agcttttaat cctgcaaaat
     4021 gggatacttt acccaattcg tattcaatgc gcataagctg gttgaatttt tctatgcgct
     4081 cactgcggca tccgcttcct gtttttatat ggcctgcatt tacggcaacc gccaggtcgg
     4141 caataaagct gtccgaggtc tccccgctgc ggtgtgatac aaagcaatta taagaattcc
     4201 tgttggccag ttcaattgtt tccagtgttt ctgttacaga acctatctgg ttcagtttga
     4261 tcaggacgga attggccaga ccttttttga tcccttctgc caggatggct ttgttggtac
     4321 agaagaggtc atctcctacc aattctattt tgttcccgat ggtgtcggtc aggttcttcc
     4381 atccctgcca atcgttttct gcaagtccgt cttctatgga aacaatcggg tattgcctta
     4441 cccagctttc ccatatcttc accatttcat cgctcgatac cagctgtttt gtactcttga
     4501 aaaatttgta tttcccgtct tcccacattt cactggcggc aggatcaaga caaatgctta
     4561 catcttctcc cggtttgtac cctgctttta cgatcccttc caggatcatt tccacggcct
     4621 gctcgtttgt cttcagatcc ggggcaaatc cgccttcgtc tccaacaccg gtactcaggc
     4681 cttttgattt aagaacgctt ttcagggcat gaaaaacctc ggcacccatc cgaatggatt
     4741 cacggaatga aggagcgttg tggggggcga tcatgaattc ctggaaatca actgtattgt
     4801 cggcgtgggc accgccattg atcacattca tgcagggaac gggcaaaaga tgggcattgc
     4861 tgcctcccag gtattggtaa aggggaatac ccagacttat ggacctggcc cttgcatacg
     4921 ccatggaaac agccagaatg gcattggcgc caagttttcc cttgttgggg gttccgtcca
     4981 ggtctatcat aaggtaatcc aactccctct ggcttgtaaa gcacttaccc tcaatagctt
     5041 ttgcgatgtt ataatttaca ttggaaacag ctttaagcac tccttttccg ttatagcgct
     5101 ttttgtctcc gtcgcgcagt tcaacagctt ccctttcacc ggtagatgcc ccgctcggca
     5161 ccatggcagt tgccctggta ccatcttcca gtgacatcct gacctctact gttggattgc
     5221 ctcttgaatc caatatctca atagcttcaa cttttttgat tttcattata tgatcctcca
     5281 gaattaaatg attatatcct gcaaatttac agtataaaaa tcaatccgga aaagaaccgg
     5341 tataaaaaaa ccgcgatgtt atcacggtta ctggatgtgg tgccaccggg aatcgaacca
     5401 gggacacaag gattttcagt cctttgctct accaactgag ctatggcacc attcttgaat
     5461 gggggaacaa agttaaatat taatttacag ccggcaaaat tttttatttt ttaaactatt
     5521 gatgccttag tccatcaaat ggatacattt gtcaaatttt accatttcat actttttgaa
     5581 tcaacatgaa aaagaggaat atcatcattt tgagcgctgt ggccgtcgtg gccattgtgc
     5641 tcatcatcat ctttgccagc ggaaacaagg atggttatgt accccaggta gtcaaggctc
     5701 aaa
//
LOCUS       DAOSOF010000121         5648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14474_length_5648_cov_5.224924, whole genome shotgun sequence.
ACCESSION   DAOSOF010000121 DAOSOF010000000
VERSION     DAOSOF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14474_length_5648_cov_5.224924"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2626)
                     /locus_tag="PLM68_07060"
     CDS             complement(<1..2626)
                     /locus_tag="PLM68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT55153.1"
                     /translation="MKKLLILLFLGLSCSAFAQTVKVSGRVTGSDGTSLPGVYVMEEN
                     TTNATSTDLDGNFTLDVPVGSNLLFSYIGYRTQIIPVTGAAVLSVILEEDSALLEEVV
                     VVGYGTQKKSVVTAAIAKVSSDDLKNVAPVRIDNALKGLTSGVTVTSSSGQPGASARV
                     RVRGVGTINNSEPLYIVDGMPIGGGIDYLNPSDIQSIEVLKDAASGAVYGARAANGVI
                     LVTTKSGQKGATRVTYDFSYGFQNPWKEYDVLNATEYALLMNEGYLAQGKAPVYSDPY
                     ALGEGTDWQKEVFNYNAPQINHQLTISGASDKVNYYISAGYYSQDGIVGGNFNRSNYE
                     RLTIRTNTTYNLMDQSAKRNFLHKFTMGINAAYSRNTSFGITTNSEFGGPLGSALGMA
                     PTLKVYADDPEALLAEHPAAVKDPRNGKPFSIVEGSIYNEMVNPLASLSLPGTKGNSD
                     KLVSNFWGELSIWDNLKIRSSLGIDLSFWGDDGWSPAYFLSTKNFRDYSEVWSSMNRS
                     LVWQVENVLSYEKQFGSHFIQALVGQSAQGNMGQNLAGGNRYLQEEDPYKANMGFATG
                     TQEYQTASGYVYTPHRLASLFARVSYNFAERYMLQATVRRDGSSNFGPNNLYAIFPSA
                     SVGWNFSNESFLANRPQWFSSGKLRASWGKNGNESIGQFKYTTTMASGNNYPFGNPGV
                     VNTGAKPNGFANADIRWEESTQTDIGLDLAFFNSALAFSMDWYKKKTTGMLMDIPVPA
                     YSGDSAPTGNVGIMDNSGWEFDIRYRFSLGDAHFNIGANATYLDNLLVELGNEKGWQN
                     YDSHKIGTLTRAQNGYPFPFFYGWKTDGLFQNWAEVEAGIQPNAQPGDVRFVDIDNNG
                     VINDDDRTMIGKGMPDWSF"
     gene            complement(2794..4446)
                     /locus_tag="PLM68_07065"
     CDS             complement(2794..4446)
                     /locus_tag="PLM68_07065"
                     /inference="COORDINATES: protein motif:HMM:NF039744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HOT55154.1"
                     /translation="MKQNGLTAFFLIVSLLMLRGENRQQLTRLLEDLDKQLELRTTYE
                     QNRLQRINALKSVIQQQDLSPEQSYGFYQQLIEEYKAYNFDSTMHYIEASAHLASLLN
                     SKPYQDQTRIRRGLIFATSGNFLESSDILYNDIDSLTLDPGLLPEYYVALQRLSGELA
                     EYSTDPATKRIYTGRRAYYRSKIMEQTDPQSDEYLYYLFQDAYETGNLSRADSLSAVL
                     VSRHKESSHSYAIYAYHRSTVFQNRGEVGSQMEWLARSAMADIQSAVKDHASLTRLAT
                     VLFETGGNIDRAFRYIDISLADALFYNAKLRPWQVAGVLPQIEKAYQEKQVTQARRAR
                     AFLISISALVLVLVLASAYLVKQTRRTIRAHSSLREINKRIVTQNKELNRVNVQLQTL
                     NHQIAESNRVKEGYIGLFLAILSDNLDKMKEFRAYVKRSIRYGKTQELSEELNNTEWA
                     EEEVANFYRTFDTAFLELYPSFVQDFNALLTPEEQIVLKKGELLNTELRIFALIRMGI
                     KDSGKIASLLRYSVNTIYNYRAKIKNAALGSRDDFEDRVSSL"
     gene            complement(4459..>5648)
                     /locus_tag="PLM68_07070"
     CDS             complement(4459..>5648)
                     /locus_tag="PLM68_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT55155.1"
                     /translation="YNYGDGSTFPQYKHLMYVEANYTTKRSGLFRFFYDSKYLIPGVR
                     VTLDLSYVPEAMADFLGFNGYRSVYNPVWGKTGETGYLSRAFYKMKKDFFRATADFQG
                     TIAGNLSWNAGLGVFNYRADEVNIDFINRNKAEEKKLPTTDVQPTLYNYYAQWGLIKA
                     NELHGGTHPFVRAGLTFDSRNRPNNPNRGIWADAFLTYFGAFGEQKEYNHLTLNASFR
                     HYVPVWGDRIIFAYRLGYQGSLWGNTPFYMKNYMATLFTKRANYEMLGGANSLRGIVR
                     NRVTGDGYAYANVEFRVKLVSFKIARQAFYIGINPLFDIGYITDQVEWNRSEAELRAL
                     TAASGVRYEDFFSDKDGWHMSAGAGLKIAMNENFILSVDFAKAFRKQDGNTGLYIMLG
                     YMF"
BASE COUNT         1308 a         1417 c         1477 g         1446 t
ORIGIN      
        1 cgaatgacca gtcgggcatt cctttgccaa tcattgtcct gtcatcgtcg ttgattaccc
       61 cgttgttgtc aatgtccaca aaacgtacgt ctccgggctg ggcattgggc tggataccag
      121 cttcaacttc tgcccagttc tggaacaggc cgtcggtttt ccaaccgtag aagaagggga
      181 agggatagcc gttctgagcg cgggtaagtg tccctatctt gtgcgagtcg taattctgcc
      241 atcctttttc gttccccagt tccaccagca ggttgtccag gtaggtggcg tttgccccga
      301 tgttgaaatg agcatcacca aggctgaagc ggtacctgat atcgaattcc cagccgctgt
      361 tatccatgat acccacgtta cctgtgggag cactgtcgcc cgaataggca ggaaccggga
      421 tatccatcag catgccggtg gttttcttct tgtaccagtc catggaaaac gcgagtgcgc
      481 tgttgaagaa tgccaggtcc agccctatgt ccgtctgggt ggattcttcc cagcggatat
      541 cagcgttggc aaaaccgttt ggtttggctc ccgtattgac tacacccgga ttgccgaacg
      601 ggtagttgtt acccgaggcc atggtagtgg tgtatttgaa ttgccctatg gattcattcc
      661 cgtttttccc ccaggaagcc ctcagtttcc cgctggagaa ccattggggc ctgtttgcca
      721 ggaagctttc attggagaaa ttccatccca cggaggcgga agggaaaatg gcatacaaat
      781 tattgggacc gaaattggat gaaccgtcac ggcgtaccgt ggcctgcagc atgtaccttt
      841 ctgcaaagtt gtaactgaca cgggcaaaga gggatgccag gcgatgcggg gtatagacgt
      901 atccgctggc tgtctggtat tcctgtgttc ccgtggcaaa tcccatattg gccttatagg
      961 ggtcctcttc ctgcaggtag cggttccccc cggcaaggtt ctgtcccatg ttgccctggg
     1021 cggactgtcc gacaagagcc tggataaaat gggacccgaa ttgtttttca taggatagca
     1081 cattttccac ctgccatacc agggagcggt tcatggaaga ccatacttcc gagtagtccc
     1141 ggaaattttt cgtggatagg aaataggcag gactccaccc gtcatctccc cagaaggaca
     1201 aatctatacc cagggagctt ctgattttca ggttatccca gatggagagc tcaccccaga
     1261 aattggaaac caatttgtcc gagttgccct ttgttccggg caggctcagt gaagccaggg
     1321 ggttgaccat ctcgttgtag atgctcccct ccacaatgct gaagggtttt ccgttcctgg
     1381 gatccttaac cgcagcgggg tgttcggcca gcagagcctc ggggtcatcg gcataaacct
     1441 tcagggtggg agccatgccc aatgccgagc ctaaaggtcc tccgaattcc gagttggtgg
     1501 taatgccaaa ggatgtgttc ctggaatagg cggcgttgat gcccatggtg aatttgtgca
     1561 ggaagttcct tttggcagac tggtccatca ggttgtatgt agtgttggtc cttatggtaa
     1621 gacgttcata attggaacgg ttgaagttac cgcccacaat cccgtcctgt gaataatacc
     1681 cagccgatat gtaatagttc accttgtcag aggctccact gatggtcagc tggtggttga
     1741 tctggggagc attgtagttg aagacttctt tctgccagtc ggtgccttct cccaaagcat
     1801 aagggtcgct gtaaacaggg gctttgccct gcgccaggta cccctcattc atgaggaggg
     1861 catattctgt ggcattgagc acatcgtact ctttccaggg attctggaaa ccataagaga
     1921 aatcatacgt gacgcgtgtg gctccctttt gtcccgattt ggtggtcacc aggataacgc
     1981 cgtttgcggc acgagccccg taaacggctc ccgatgccgc gtctttaagc acctctatgg
     2041 actggatatc ggagggattc aggtaatcga tgcctcctcc aatgggcatt ccgtcaacaa
     2101 tgtacagagg ttcggagttg ttgatggttc ccacaccacg cactcgcaca cgcgccgagg
     2161 ctcccggttg tcccgaggaa gaggttactg tcacaccgga agtcaaccct ttcagggcat
     2221 tgtctatcct taccggtgcc acatttttca aatcatcgga agataccttg gcaatggcag
     2281 cggtaacgac actctttttc tgggtgccgt agcccactac gaccacttct tccaggagcg
     2341 cagagtcttc ttccaggata acgctgagga ctgccgcgcc cgttacggga atgatctgag
     2401 ttctatagcc tatgtatgaa aagagcaggt tactgccaac aggcacatcc agggtaaaat
     2461 tcccgtcaag gtccgtagat gtggcattgg tggtattctc ttccatgaca tatactccgg
     2521 gcagagacgt tccgtcactc ccggtaaccc tgccgctcac tttcacggtt tgtgcgaagg
     2581 ctgaacagct aagtcctaaa aacaaaagga ttaaaagttt tttcatgttt tgttttagtt
     2641 agtttggttt agttggagat tttgataaaa atatcaatta ttcaaccccg tttttttcaa
     2701 agtggttact tagtggacag tagaatgtaa taaataaaac aaaccgctgt atatgagcgg
     2761 tttgcctttt ataacctgga gaaaaaagtg gatttaaagg gatgagaccc tgtcttcaaa
     2821 atcgtctcgt gaacccagtg ccgcgttttt gatctttgcc ctgtaattgt aaatggtgtt
     2881 gacagaatac ctcagcagcg aggcaatttt tcccgaatcc ttgatgccca tcctgatcag
     2941 cgcaaagatc ctcaattccg tattcagaag ttctcccttt ttcaggacta tctgttcttc
     3001 aggagtcagc agggcattga aatcctgcac aaaagacggg taaagttcca ggaatgccgt
     3061 gtcaaatgtc ctgtaaaaat tagccacctc ttcttccgcc cattcggtat tgttcaattc
     3121 ttcgctcagt tcctgagtct tgccgtaacg tatggagcgc ttgacatatg cccggaattc
     3181 tttcatctta tccaggttat cggaaagaat ggccaggaac agcccaatgt aaccctcctt
     3241 gacacgattc gattccgcaa tctggtggtt cagtgtctgc aattgtacgt taacgcggtt
     3301 cagctccttg ttctgggtga cgatcctttt gttgatctcc ctcagggagc tgtgggcccg
     3361 gatggtccgc cgggtttgtt ttaccaggta agccgaggca agaaccagca ccagcaccag
     3421 ggcactgatg gatatcagga aagccctggc cctgcgggcc tgggttactt gcttttcctg
     3481 gtaggccttt tcaatctggg gaagcacgcc agccacctgc cacggccgga gcttggcatt
     3541 gtagaacagg gcatcggcca gggaaatgtc tatgtaccgg aaagccctgt caatattgcc
     3601 cccggtctca aagagaacgg ttgccagccg ggtcagggat gcatggtcct tgactgcaga
     3661 ctggatatcg gccatggccg aacgggccag ccattccatc tgactgccta cctctccccg
     3721 gttctggaaa acggtggaac ggtggtatgc gtatatggcg tatgaatggc tggattcctt
     3781 atgcctggac accaacacgg cagaaagact gtccgccctg gacagattcc ctgtctcata
     3841 ggcatcctgg aacaggtaat acaggtattc atcggattga ggatccgtct gttccatgat
     3901 ctttgagcga taataagccc gcctgccggt ataaatccgc ttggtggcag gatctgtgga
     3961 atactctgcc agttctcccg acagccgctg caaggcaaca taatactcgg gcaaaaggcc
     4021 gggatccaga gtaagggagt ctatatcgtt gtatagtata tcactggatt ccaggaaatt
     4081 tcccgatgtg gcaaagataa gaccccggcg gatccgtgtc tggtcctggt acggtttgct
     4141 gttgagcagg gaggccagat gagcagaagc ctcgatataa tgcatggtgg aatcaaagtt
     4201 ataggctttg tattcctcaa tcagttgctg ataaaaacca tagctttgct ccggggacag
     4261 gtcctgctgc tgtatgacgc tcttgagtgc attgatgcgt tgcaggcggt tctgttcata
     4321 agtggtcctg agttcaagct gtttgtccag gtcctcaagc aaacgggtca attgctgcct
     4381 gttctccccc cgcagcataa gcagggaaac tatcagaaaa aaggcagtca acccgttttg
     4441 tttcatgata cgatcccgtt agaacatata acccagcatg atgtaaaggc cggtgttccc
     4501 atcctgtttc ctgaaagcct tggcaaaatc cacggacaag atgaaattct cgttcatggc
     4561 tatcttcaac ccggcaccgg cactcatatg ccatccatcc ttatcagaga agaaatcttc
     4621 ataccggaca ccggaggcgg ctgtcagggc acgtaattcc gcctcggaac ggttccattc
     4681 cacctgatct gtgatgtacc cgatatcaaa cagggggttg atgccaatat aaaaggcctg
     4741 gcgtgcgatc ttgaatgaaa ccagcttgac ccgaaactcc acgttggcat aggcataacc
     4801 gtcgccggtc acacgattgc gcacgatccc gcgcaggctg ttggccccgc ccagcatctc
     4861 ataattggcc cgtttggtga atagggtggc catgtaattc ttcatgtaaa acggggtgtt
     4921 tccccagagg gacccctggt aaccgagacg gtaggcaaag atgatcctgt ctccccatac
     4981 tggcacatag tgacggaaag aggcattcag ggtcaggtgg ttgtattcct tctgctcccc
     5041 gaaggcgcca aaataagtga gaaatgcatc ggcccagata ccccggttgg ggttattggg
     5101 gcgattgcgg gaatcaaagg tcaggccggc acggacaaac ggatgggtcc cgccgtgcaa
     5161 ttcattggct ttgatcaggc cccactgggc atagtaatta tacagggtgg gctggacatc
     5221 tgttgtaggc agcttttttt cctcggcctt gttccggttg atgaaatcaa tgttaacctc
     5281 gtcggccctg taattgaaaa cacccagccc ggcattccac gaaagattgc cggctatggt
     5341 tccctggaaa tcggcggtgg cacggaaaaa gtcttttttc attttataaa aggcgcgaga
     5401 cagataaccg gtctctccgg tctttcccca cacgggattg taaacggagc gatatccgtt
     5461 gaaacccaga aaatcggcca tggcttcggg aacataactt aggtcaagag taacgcgcac
     5521 cccgggtatc aggtatttgg aatcgtaaaa gaaacggaac aatccggatc gcttggtggt
     5581 gtagttggct tctacataca tcaggtgttt gtactggggg aatgtggacc cgtctccgta
     5641 attgtaaa
//
LOCUS       DAOSOF010000122         5585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14668_length_5585_cov_5.438336, whole genome shotgun sequence.
ACCESSION   DAOSOF010000122 DAOSOF010000000
VERSION     DAOSOF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14668_length_5585_cov_5.438336"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(436..1020)
                     /locus_tag="PLM68_07075"
     CDS             complement(436..1020)
                     /locus_tag="PLM68_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55156.1"
                     /translation="MKRISDMVNKLFDSYSPLDVPVVHRATLYGCALIGGTAVQFLAR
                     QYGVKERRSRSINDLDFITPASNPLTRHFNDWLVRSHFREIKAGASEYMWNYENPHAG
                     VEVDVLISHDPQLPSQIIKVAQILVWHPVNLFVSKVQRMTTFNTRADTDKADLNTLYD
                     ILEQRNELPLLETALSHLDLSDAEIAQVNEWIKE"
     gene            complement(992..1276)
                     /locus_tag="PLM68_07080"
     CDS             complement(992..1276)
                     /locus_tag="PLM68_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55157.1"
                     /translation="MDKTILFFIVWGIAYIVSRIRKKPGKPIPPPEPKFITSVNHIGC
                     AVQAVDQHGCIPHTSTHVSAICGSTGYVYDINGGIAYTFPVDEKDIRHGE"
     gene            1351..2004
                     /locus_tag="PLM68_07085"
     CDS             1351..2004
                     /locus_tag="PLM68_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55158.1"
                     /translation="MKTIIFVWDRKNDLIPKEDTIGKALEASAPATLLWRKWLADPRH
                     VTQAEEGDRFFIMKRDRARQGIFMSGFLDKAPRKNQHEWQFTLCPDAIIDWTREPVLD
                     SRAFPGFRFGWSADLEEAVLPREDARILELLWARHLFNAKSHVCEPNTILNEAIKIAK
                     GESCELCGEALYSKYDVFYKPEVMKPRWHELKPGDDIIANYFLLCDTCSGIQASLGK"
     gene            complement(2008..>2334)
                     /locus_tag="PLM68_07090"
     CDS             complement(2008..>2334)
                     /locus_tag="PLM68_07090"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55159.1"
                     /translation="DKLLAETYGLILYQEQLMTIAALVGNYTQFGTLPFMRTFFYGPR
                     DSALAACREEFMASARDNGYDEEKVQALWERMERFGPCTFNKSHAVCSALTSYYCAYV
                     KAHAGD"
     assembly_gap    2335..2434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2435..2848)
                     /locus_tag="PLM68_07095"
     CDS             complement(<2435..2848)
                     /locus_tag="PLM68_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55160.1"
                     /translation="METKYFNQTLRKLVYAGAARRYKDLTPAIVSRIETEVYTLCEQG
                     RAKTFVLWANLADEIWDSGMIMGLGYGAAPGSMVNYCLNITHVDPLPYNLLFERFFDP
                     TPGKTLEVILDVENDYVETVTRLIAGMRQEEGSSIK"
     gene            complement(2854..4362)
                     /locus_tag="PLM68_07100"
     CDS             complement(2854..4362)
                     /locus_tag="PLM68_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT55161.1"
                     /translation="MNAKIYRGAEEIGGNCIALQTAQTKILVDFGLPLDFDDRPKEEQ
                     KKIWAEAEAWAQDAQAVFITHSHPDHYGLMPALPKGTPVFMSEGTRILLASNPFNGID
                     TDDYTIITFAYRQPFTFRDITVTAYAVDHSAYGACAFCFQAEGKSVLYTGDIRLHGRK
                     GVLYKLLPKHVDCLFLEGTNVRVDEGKNVVKEEGTNLGTDEIRNIGIDKNANLGKEEG
                     TIVQVDSNLGQNNATYFSNNKKCARETALTEKFAALFTEEKALAKDKSFGKETEFSKD
                     AAFTQGTASAKAAESLHLVWCSGQNIDRIVTLYKACLRSGRQLVVDPYTAVVLDEVAK
                     LSPKIPNVLGFNSVKVYYPQRLTTCLNKKCGSRYTTYLQPRVNKITYKDINANPGAYV
                     MVVRESTLDYIQRLSVPSMRFTISTWRGYWDDVNSSKTARFREWIEKHCEITEDIHTS
                     GHADAYGLKRIVEHVQPKGIVPIHTENAKLFSQITGNTTPVFVLYNNATFEI"
     gene            complement(4756..>5585)
                     /locus_tag="PLM68_07105"
     CDS             complement(4756..>5585)
                     /locus_tag="PLM68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT55162.1"
                     /translation="RYIWLMSTIYDAGRISYEEIEKKWLRSSLNDQRKPFPLRTFHDH
                     VNAIQELFDVNIVCDRSYGYKYYIENADVLDLDSVCLWMLNSFSVSNAIAGAKDIRDR
                     ILIQDIPSSRRWLAPILSAIREKQVIEIDYLSFQKGEVPARKLCPFYVKLYDNRWYMF
                     AREPENPKMKVYALDRIKDLRTLDEEFSFEPTPADRESIDKCFGHCIYEDIPPQVIRI
                     KATKSASYYLDTLPLHKSQRIHEVGEDYVIYEYEFAPTTEFQATLRKWGNQLEICKE"
BASE COUNT         1267 a         1297 c         1426 g         1495 t
ORIGIN      
        1 atcatcatac ttatttttga aacttcaaat tgccactttt taaaggggac tcatttttga
       61 aacttcaaat tgccactttt taaaggggac tcaatcttta aatataccta atcaatcaga
      121 gacgtgaaat attttttatg attccggtcg gtttgggttt gaattcggtc ggtttggatt
      181 tgaattcggg aaaatatgag aatttttgtc ctgccggatt tttcactttc aggacaattt
      241 tcgaggacat tttaaagctt gtttagtggt gaaattttgg cttttcaaac agcatggccg
      301 taccgtctag caggcatccc agtatcagta gtattatcat caacataaca ggcaagtgtt
      361 taatgggttg gtaattgatt caaaaataag ggagcactac gtcaaaatac gacgtagtgc
      421 tgcaggtaaa gggaatcatt cctttatcca ctcattcacc tgcgctattt cagcatcgga
      481 taggtcaaga tgggacaagg ctgtttccag caggggaagt tcgttgcgtt gttccaggat
      541 gtcgtagagt gtattcaggt ctgccttgtc ggtgtctgcc ctggtgttga aggtggtcat
      601 ccgttgcact ttggatacga acaggttgac cgggtgccag accaggatct gggccacctt
      661 gatgatttga cttggcaatt gtggatcatg ggatatcagc acatcaactt ccacgccggc
      721 atgggggttt tcataattcc acatgtattc cgaagcgcct gccttgattt ccctgaagtg
      781 gctccttacc agccaatcat tgaaatgcct ggtaagggga tttgatgctg gagtgatgaa
      841 atccaggtca ttgatagagc gggaccttcg ctctttgacc ccgtattgcc tggctaggaa
      901 ttgcacggcc gtacccccaa tcagggcaca accgtaaagt gtggccctat ggacaaccgg
      961 aacatcaagc ggtgagtaag agtcgaataa tttattcacc atgtctgata tccttttcat
     1021 ccacgggaaa cgtgtatgcg atgcccccgt tgatgtcgta gacataaccg gtgctgccgc
     1081 atatggcaga cacatgggtg gatgtgtggg gtatgcatcc atgttggtca acagcctgta
     1141 cggcacagcc aatatggttg acgctggtta tgaatttggg ttcgggagga gggattggct
     1201 tgcctggttt cttcctgata cgggaaacaa tgtatgcaat gccccatact atgaagaaca
     1261 aaattgtctt atccatggcg ttatgattta atttttattt atgcggactt accgcattga
     1321 ttttgagtat atttgtactt aaaaaaagtt atgaaaacaa tcatattcgt ctgggaccgg
     1381 aaaaacgatc tcatccccaa agaggacacc ataggcaagg cattggaggc gagcgcaccc
     1441 gctaccctac tgtggcgcaa gtggctggca gacccaaggc atgtcacgca ggcagaggaa
     1501 ggtgacaggt tcttcatcat gaaaagggac cgggccaggc agggcatttt catgagcggg
     1561 ttccttgata aagcaccccg caaaaaccaa cacgaatggc aattcacgct ttgccccgat
     1621 gccatcatcg actggaccag ggagcctgtc ctggacagcc gggcattccc ggggttcagg
     1681 ttcgggtggt cggcagacct ggaagaagcc gtccttcccc gggaggatgc caggatcctg
     1741 gagttgcttt gggcacggca tctcttcaat gccaaaagcc atgtctgtga accgaatacc
     1801 atactgaatg aagccataaa aattgctaaa ggggaaagtt gcgaactctg tggtgaagcg
     1861 ctttatagta aatacgatgt gttttacaag cctgaagtca tgaagccccg gtggcacgag
     1921 ctaaagccgg gggacgacat catcgcgaac tacttcctgc tgtgcgacac ttgctccggc
     1981 atccaggcct cgctgggaaa gtgaccctca atcgcccgcg tgtgccttca cataggcgca
     2041 atagtaggaa gtgagggcgc tgcagacggc gtgcgatttg ttgaaggtgc agggcccgaa
     2101 ccgttccatc cgctcccaca gtgcctgcac tttttcctca tcgtaaccgt tgtccctggc
     2161 cgaggccatg aattcctccc tgcaggcggc cagcgcgctg tccctgggac cgtagaagaa
     2221 ggtgcgcatg aagggaagag tcccaaactg agtataattc cccaccaaag cggcaatggt
     2281 catcagctgt tcctggtaga ggataaggcc gtaagtctcg gcaaggagct tgtcnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttgat ggaactgccc tcttcctggc
     2461 gcatgccggc aataagccgg gttacggttt ctacgtagtc attttcaaca tccaggatca
     2521 cttccagcgt tttgccgggt gtgggatcaa agaagcgctc aaacagcagg ttgtacggga
     2581 gggggtccac gtgggtgatg ttcaggcagt agttcaccat ggagccggga gcggccccgt
     2641 agcccaaccc cattatcatc ccgctatccc agatctcgtc tgccaggttg gcccaaagga
     2701 caaaggtctt ggccctgccc tgctcacaaa gagtgtacac ctcggtttct atcctgctca
     2761 cgatggcggg ggtaaggtcc ttgtagcgcc tggcagcgcc tgcatagacg agcttgcgta
     2821 gtgtttggtt gaagtatttt gtttccatgg gcgttaaatt tcaaaagtgg cgttgttata
     2881 taatacgaag acgggggtcg tatttccggt tatttgggaa aaaagttttg cattttctgt
     2941 gtggattggc acaattccct taggctgaac gtgttccaca atgcgtttta gaccgtatgc
     3001 gtcggcgtgg ccgctggtgt ggatatcctc ggtgatttcg caatgctttt cgatccattc
     3061 ccggaatctc gcggttttgg agctgtttac gtcatcccag tagccccgcc aagtggaaat
     3121 ggtaaagcgc atggatggca cagacaggcg ttgtatgtag tccagggtgg attcgcgcac
     3181 gaccatcacg taggctccgg gattggcgtt gatatccttg taggtgattt tgttgacccg
     3241 aggctggaga tacgtggtgt acctggaacc gcatttcttg ttcaggcagg tggtgagcct
     3301 ttgaggatag tatactttca cgctgttgaa tcccagcacg ttcggtattt tcgggctgag
     3361 ttttgccacc tcgtccagga caacagccgt gtaaggatcc accaccagct gccggccact
     3421 acgcaggcag gccttgtaga gcgtaactat ccggtcaatg ttctgaccag aacaccacac
     3481 caggtgcaag ctctccgcag cttttgcgga tgctgtgcct tgtgtgaatg cagcgtcttt
     3541 tgagaattct gtttcttttc cgaatgattt gtcttttgct aatgcttttt cttctgtgaa
     3601 taatgcggca aatttctccg tcagtgcggt ttcccttgcg cattttttat tgttgctgaa
     3661 gtaggttgca ttgttttggc ccaggttgct gtccacctgc acgatggttc cttcttcttt
     3721 gcccagattg gcgtttttgt ctatgcctat atttcttatt tcgtcggtgc ccaggttcgt
     3781 cccttcttcc ttaaccacgt tcttcccttc atccacgcgc acgttggtgc cttccaggaa
     3841 aagacaatcc acatgttttg gcagcaactt gtaaagcacg cccttccgcc cgtgcagcct
     3901 gatgtccccg gtgtacagga cagacttacc ctctgcctgg aagcagaagg cgcatgcccc
     3961 gtaggccgaa tggtccacag cgtaggcagt gacagtgatg tcgcggaagg tgaacggctg
     4021 ccggtaggcg aaggtgatga tggtgtaatc gtctgtgtcg atgccgttga acgggttgga
     4081 tgccaaaagg atcctggtcc cttcactcat gaacaccggt gtgcccttgg gcagggcagg
     4141 catcaggccg taatggtcgg ggtggctgtg ggtgatgaag accgcctggg cgtcctgggc
     4201 ccaggcctcg gcctcggccc agatcttctt ttgttcctct ttggggcggt catcgaaatc
     4261 caaaggaagg ccaaaatcca ccagtatttt ggtctgcgcg gtctgtaagg caatgcagtt
     4321 tccgccaatt tcctctgcgc ccctgtaaat ctttgcattc ataacttcta gttttaatgc
     4381 aaagatgaac ggaaagtgtg cgacaaggcc gcagaggcga aaagaggtgg aacctccagc
     4441 gccaaatcca agatgatatt ttattaatta cctgtaaaac agctgcatat aatatatcta
     4501 cgtcaaaaat ggcgcatgag gttccacctg tttcatttct attgtgcaaa ttttaggcgt
     4561 ttcattgtgc agatttgagg tgtttcacgg acggtatgtg gtcaaatggc agagattcag
     4621 ctaataatct tgattgttat aatgagtatc ttttactcac gtttcaggaa catgggcatt
     4681 atcttgtttt atttacccat gtttatgcaa catggccatt acgtacccac gttcaataat
     4741 tgttaattat ttcatttact ccttgcaaat ttcaagctga ttgccccatt tacgaagggt
     4801 ggcctggaat tccgtggttg gagcaaattc atattcgtaa atcacatagt cctcccctac
     4861 ttcgtggata cgctgggatt tgtgcaaagg cagggtatcc aggtagtagg aagcagattt
     4921 ggtggctttg atgcggatta cctggggtgg gatatcttcg taaatacagt gcccaaagca
     4981 tttgtctata gattcccggt cggccggggt aggctcgaat gaaaactcct cgtcaagcgt
     5041 tctaagatct ttgatcctgt ccagcgcata taccttcatt tttgggtttt ccggttcccg
     5101 ggcaaacatg taccagcggt tgtcatagag cttaacatag aacggacaga gtttcctggc
     5161 aggaacctca cccttctgga aagagaggta gtcaatttca ataacctgtt tctcgcgaat
     5221 ggcagaaaga atgggcgcca gccaacgtcg ggaagatggg atgtcctgta tcaggatccg
     5281 gtcccgtatg tccttggccc cggcaatcgc attgctgaca gagaagctgt tcagcatcca
     5341 caggcagaca gagtccaggt ccaggacatc cgcgttctct atgtaatatt tatacccgta
     5401 actcctgtcg cacacgatat tcacgtcaaa taactcctgg atggcattga catggtcgtg
     5461 gaatgtcctc aacggaaagg gcttcctctg gtcgttcaga ctgcttcgca gccacttctt
     5521 ttcaatttcc tcataagata tccggccggc atcataaatg gtcgacatca gccagatata
     5581 tcttc
//
LOCUS       DAOSOF010000123         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14726_length_5568_cov_4.158353, whole genome shotgun sequence.
ACCESSION   DAOSOF010000123 DAOSOF010000000
VERSION     DAOSOF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14726_length_5568_cov_4.158353"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLM68_07110"
     CDS             <1..297
                     /locus_tag="PLM68_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT55163.1"
                     /translation="VLNYYTVNSLMMNCLPNDNTTAGGYGCGLEYANNLGYGHNGVHQ
                     GYLTFMVYDPLTDFTIVVYTNAWNLNDGMASVSYQLTNMLLNICYRSKELVTRH"
     gene            300..2783
                     /locus_tag="PLM68_07115"
     CDS             300..2783
                     /locus_tag="PLM68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT55164.1"
                     /translation="MSQKGHIDGIRLRVLGHRGKILILVVLFLAGSACFTGLSAQGKP
                     SLQGQQQQSKPSQQEQQQQSKPSQQEQQQQSKPSQQWLDQKYSMFIHFGLYSVYGGVY
                     DGKPVTSGYSEQIQSFAGISSDGYSVTATRFNPVNWDPDAVVALAKRAGMRSIVFTAK
                     HHDGFCLYHSLHTGFNIVDATPYGRDLLKELAEACERGGLGFGVYFSLIDWHFPQAYP
                     ISGHNADPLTPEHYRFNLAQVEELMTRYGTISEIWFDMGSLTPEQSRGLYALVNRLQP
                     GCMVSGRLGNDAGDFCVMADNDCPGYQLAVPWQTAASAFRETWGYRSWQERGALQPKV
                     EEKIGSLVQVIGRGGNYLLNIGPRGDGSLVEFEQALLEGVGDWVKVNAEAIYGTRANP
                     FGQIFSWGEVTARKDTLYLFVKRDYAGRQIELPLVEGVVSNVNVLASGQEVSFWPAGH
                     EEEKRSAGNEEKIQPAWDKEKRSAGKGNRGLSINMPEQFSSCHEVLRVTFRNGFKVIP
                     FRVVRDNILTARNAMPRYGYSSLNYYAGYKSLIAYHWAFTSEKREINPEILFTENEKG
                     KTLCIEIDGTTQEITLDSPLCRTETSGINTVITETNSAIPAANKVTQETSSVTWGNLY
                     RKPGRGVFGFLEEETAGALAVAGAAASAETGAEAGSVAEAGTGAAAGLIDPGQGWEQV
                     AGFAYGKKHLIPLGQRKSVVFMQEILSDREQTVAVEIGSGNAVYMLLNGEYLTAHFSP
                     ERVSYQTEMVLLPLQKGNNQLVIKYYNGFESELHFSITPLEEWKIYSQKLSPVTLSTE
                     GIHSLSIRESNPVSITAPLRMHNVQIKCL"
     gene            2786..4339
                     /locus_tag="PLM68_07120"
     CDS             2786..4339
                     /locus_tag="PLM68_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT55165.1"
                     /translation="MRQHLTSLILILLSLPLAAQEHFTNPLFPGDYPDPSILVDGEDF
                     YMVHSSFEYYPGLTIWHSRDLANWTPVTSALTKYVGSVWAPDLVKSDGKYYIYFPASN
                     TNYVIVAPSIEGPWSDPVELKISHIDPGHVEDGMGNRYLYFSSGGYVPLAQDGLSVTG
                     PLQHVYDGWPIPPEWVIECFCMESPKLFKRGDYYYLTVAQGGTAGPATGHMVISARSR
                     SPLGPWENSPYNPIIRAETNQEKWCSVGHATIFEASPENWWMVFHGYENGHYNRGRQT
                     LMAPVEWTADGWYRIPESVDLEQPIKRPLPSAGLVEYCLNDTFEESRLKTQWKFFGQY
                     DPARFSLPGSESHQCQQNEPCTDIQSCTETHTGAGLIIKGKGHSVAESSPLLCIPAHN
                     SYSARVEMEIEGDAIGGLMLFYSQKIFSGILADKSDILANLRGWQFPTQVDVIRRRVF
                     LRLDNLDDVVNMYYSTDGEQWVKIENSADVSAYHHNVLSEFLSLRIGLVSMGDGLVRF
                     RNFKYDKLE"
     gene            4368..>5568
                     /locus_tag="PLM68_07125"
     CDS             4368..>5568
                     /locus_tag="PLM68_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55166.1"
                     /translation="MNKKILTGVVWALIIGFAITVLVSGVAETISWGWLPAFLIALAM
                     LLLLFSAASGAVGLFKLISRIDRWLLIALGFGLAVAFLFFNNGVPAALMVFAAIAIPC
                     ALIGIGVQLLRRERAEVKRAQRILSVVSLVVGICALAGGLVWILYPGKPVDMPQNAAL
                     SAENLPPVLEMENPGETGHLEVCTLSYGSGKDKHREVFGADADLITEPVDGSTFLDSW
                     KGLSGKLRTWYFGFDKKELPLNAQVWYPQGEGPFPLVLIVHGNHQAQDFSDPGYEYLG
                     RLLASRGYILASVDENFLNGSLTNIFKGLQGENNARGWVLLKHLQVWKKWNLEEGNPF
                     YGKVDMERIALIGHSRGGEAVGHAALFNRLPYYPDNANEVFDFNFNIRSIIAIAPCDG
                     QYQPGRMR"
BASE COUNT         1332 a         1362 c         1577 g         1297 t
ORIGIN      
        1 gtcctgaatt attataccgt gaacagcctg atgatgaatt gcctgcccaa cgataacact
       61 acagccggag gttacggatg cggcctggaa tatgccaata acctgggtta tggtcacaac
      121 ggtgtacatc agggatacct tacttttatg gtttatgacc ccctgacgga tttcacaata
      181 gtggtatata ctaatgcatg gaacttgaat gacggcatgg ccagtgtctc ttaccagttg
      241 acgaatatgc tactgaatat ttgctaccgg agcaaagaac tggtaaccag gcattaataa
      301 tgagccaaaa aggacacatt gacgggatca gactccgggt ccttggccat cggggcaaaa
      361 tcctgatcct ggttgtcttg ttcctggctg gcagcgcctg tttcacaggg ctttcagctc
      421 agggaaagcc ttcactgcag gggcagcagc agcagagcaa gccttctcag caggaacagc
      481 agcagcagag caagccttct cagcaggaac agcagcagca gagcaagcct tcacagcaat
      541 ggcttgacca gaagtattcc atgttcattc attttgggct gtattcggta tacgggggag
      601 tgtatgacgg caaacctgtt acaagcggtt acagtgaaca gatccagtct tttgccggca
      661 tttccagcga cgggtactct gtaaccgcaa cccgtttcaa tcccgtgaac tgggatcccg
      721 atgctgtcgt ggcgttggcc aaaagggcag ggatgaggtc cattgtcttt acggccaagc
      781 atcacgacgg cttttgcctg taccattccc tccataccgg cttcaacata gtggatgcga
      841 cgccttacgg cagggatctt ttgaaggaac ttgccgaggc atgcgaaagg ggtggtctcg
      901 gctttggggt gtatttctcg ctcatcgact ggcactttcc ccaggcttat cccatatcgg
      961 gccacaacgc cgatcccctc accccggagc actaccggtt caacctggcc caggtggagg
     1021 agctgatgac gcggtatgga accatttcag agatatggtt tgacatgggg tcgctgaccc
     1081 cggagcagag ccggggattg tacgcgttgg tgaacaggct gcagcccggt tgtatggtca
     1141 gcggaaggct cgggaacgat gcgggtgatt tttgcgtgat ggccgacaat gactgtcccg
     1201 gttaccaact ggctgtgccg tggcagacag cggcctcggc tttcagggag acatggggtt
     1261 accggtcatg gcaggagcgg ggagcgctgc aacccaaagt ggaggagaag atcgggagcc
     1321 tggtgcaggt gatcgggcgg ggaggaaatt acctgcttaa tatcgggccc cggggcgatg
     1381 gaagccttgt ggagtttgag caggcgttgc tggaaggggt gggcgactgg gtgaaagtta
     1441 acgccgaggc tatttatggc acccgggcca atcctttcgg gcagattttt tcctggggcg
     1501 aggttacggc aaggaaggac acgttgtatc tttttgtgaa gcgtgattac gccgggcggc
     1561 agattgaact gcctttagtg gagggtgttg tatcaaacgt gaatgtgctt gcatctgggc
     1621 aggaagtaag tttttggcct gcagggcatg aagaggagaa gcggtcggcg gggaatgaag
     1681 agaagataca gcctgcctgg gataaagaga agcggtcggc gggcaaaggg aaccggggtt
     1741 taagcatcaa tatgcccgaa cagttttctt cctgccacga ggtactaaga gttacattca
     1801 gaaacggttt taaggttata ccttttcgag tagtgagaga taacatattg acggcccgca
     1861 acgccatgcc ccgctacggg tactccagcc tgaactacta tgcgggttac aagagcctga
     1921 tcgcttatca ctgggctttc acctcggaaa aaagagagat caacccggaa atccttttta
     1981 cggaaaatga aaaaggaaaa actctctgca tagagattga cggtaccacg caggagatta
     2041 cattagattc tccgctttgt cggacagaaa cgtcagggat taacacggta attactgaaa
     2101 ctaactcggc aatcccagcg gccaacaaag taacccagga aacaagcagt gtgacctggg
     2161 gcaacctcta ccggaaaccc ggccggggcg tcttcggatt tcttgaagag gaaacggccg
     2221 gggcacttgc cgttgcggga gcagcggcct cagctgaaac cggagcagaa gctggttcag
     2281 tagccgaagc cggcacagga gctgctgccg ggctcattga tcccgggcag ggttgggagc
     2341 aggtcgcagg ttttgcgtat ggcaaaaagc atttgatccc actagggcag cggaaaagcg
     2401 tggtcttcat gcaggagatt ttatctgacc gggagcagac agtggctgtg gaaatcggga
     2461 gcgggaatgc cgtatacatg cttttgaacg gggaatacct tacggcacat ttttcgcctg
     2521 aaagggtaag ctaccagacg gaaatggtgc tcctgccatt gcagaaagga aataatcaac
     2581 tggtgatcaa atattacaac gggtttgaaa gtgaactcca cttcagcata accccgcttg
     2641 aggaatggaa gatctactcc caaaagcttt ctcccgttac cctctcaact gaaggaattc
     2701 actccttatc catacgggaa tcaaatcccg tttccataac ggctcccctg aggatgcata
     2761 atgttcaaat caaatgtttg taaaaatgag acaacatctt acctccctga tccttatcct
     2821 gctgtctctg ccacttgcgg cgcaggagca ttttaccaac ccgctgttcc cgggtgatta
     2881 tcccgatcca tccatcctgg tagacgggga ggatttttat atggtgcatt cttcatttga
     2941 atattatccg gggctgacca tctggcattc acgggacctg gccaattgga ccccggtcac
     3001 cagtgccctt acaaaatacg tgggctctgt ctgggctccg gacctggtca aatctgacgg
     3061 aaagtactat atctattttc cggccagcaa caccaattat gtaatagtgg ccccttccat
     3121 tgaaggtccc tggagtgatc cggtggaatt aaagatcagt catatagatc cggggcacgt
     3181 ggaagacggg atggggaacc ggtacctgta tttcagcagc ggaggctatg tgcctttggc
     3241 acaggatggt ttgtcggtaa ccggcccgtt gcagcatgtg tatgacggct ggcccattcc
     3301 ccccgaatgg gtcattgaat gtttctgcat ggaatcgccc aagctgttta aaagaggtga
     3361 ttattattac cttacggttg cccagggtgg aaccgcagga ccggcaacag ggcacatggt
     3421 catctcggcc cgttcaagat cgcccctggg accatgggaa aactcacctt acaatcccat
     3481 catccgtgca gaaacaaacc aggagaaatg gtgctcggta ggtcatgcca caatttttga
     3541 ggcatccccc gaaaactggt ggatggtttt tcacgggtac gagaacgggc actacaaccg
     3601 gggtcgccag acgttgatgg ctccggtgga atggaccgcc gacggatggt accgtatccc
     3661 ggaaagtgtt gatttggaac aacccataaa acggccgctg ccctcagccg gcctggtgga
     3721 atactgcctg aacgacactt ttgaagagag ccggctgaaa acccagtgga aatttttcgg
     3781 ccagtacgat cccgcccgct ttagtctccc gggctctgaa agccaccagt gccaacaaaa
     3841 tgagccctgc accgatatac agtcctgtac cgaaacacac acaggcgccg gtcttatcat
     3901 caagggcaaa ggtcattcag tggcagaaag ctcgccgctg ctctgcatcc ccgctcacaa
     3961 ttcctactcg gcccgggtgg aaatggagat tgaaggcgat gccattggcg gactgatgct
     4021 gttttacagc caaaagatct tttcaggcat cctggcagat aagagtgata tcctggccaa
     4081 tttgcgcgga tggcaattcc ctacacaggt ggatgtgata cgccgccggg tgtttttacg
     4141 ccttgataac ctggatgatg tcgtgaacat gtattatagc acagacggcg agcaatgggt
     4201 caagattgaa aactcggccg atgtttctgc ttaccatcac aatgtgctga gcgaattttt
     4261 aagcttgagg attggactgg tatccatggg ggacggattg gtccgattca ggaattttaa
     4321 atacgataaa cttgaatagc actgtataat taatacatta ttacaaaatg aataaaaaaa
     4381 tcctgacagg tgtcgtatgg gcacttatta ttggctttgc cattaccgtg ctggtctccg
     4441 gcgtggccga gacaatttcc tggggatggc ttcccgcctt tcttatcgca ctggccatgc
     4501 tcttgctgtt gttttctgcg gcctcgggtg cggttggcct tttcaaattg atctcccgca
     4561 ttgatcgctg gcttttgatc gccctgggat tcggcctggc tgttgcgttc ctgtttttca
     4621 ataacggcgt gcccgctgcc ctgatggtat ttgcagccat tgccataccc tgtgccctga
     4681 tagggatagg ggtgcagctg ttgagaaggg aacgggccga ggtgaaaaga gcgcagcgga
     4741 tcctgtccgt tgtgagcctg gtggttggca tctgcgccct ggccggtggc cttgtgtgga
     4801 tcctttatcc ggggaagccc gttgatatgc cgcagaacgc ggccctctca gcagaaaacc
     4861 tgcctccggt cctggaaatg gaaaatcccg gcgaaacggg gcacctggag gtatgcactc
     4921 tgtcctacgg gagcggaaaa gacaagcacc gggaggtttt cggagctgac gccgacctga
     4981 tcacagagcc ggtggacggc tccacttttc tggattcatg gaagggcttg agcggaaaat
     5041 tgcgcacctg gtattttgga tttgacaaaa aagaattgcc acttaacgcg caggtctggt
     5101 atccgcaagg tgaaggacca ttcccgctgg tgctcattgt acacggtaac caccaggcgc
     5161 aggacttttc cgatccgggg tacgaatacc tcggcaggtt gctggcttcc aggggatata
     5221 tcctggcctc ggtggatgaa aattttctga acggttccct tactaatatt ttcaagggac
     5281 tccaaggaga gaacaatgcg cgtggctggg tgctgctcaa acacttgcag gtatggaaga
     5341 agtggaacct ggaggagggc aatcctttct acgggaaagt ggacatggag cgcatagccc
     5401 tcattggcca ttcgcggggt ggggaagccg tgggccatgc ggcgctgttc aacaggctgc
     5461 cttactatcc cgacaatgcc aacgaggtgt ttgatttcaa tttcaacatc cgttcgatca
     5521 ttgcaatcgc cccttgtgac ggacaatacc agcccggacg catgcgca
//
LOCUS       DAOSOF010000124         5534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14818_length_5534_cov_5.482387, whole genome shotgun sequence.
ACCESSION   DAOSOF010000124 DAOSOF010000000
VERSION     DAOSOF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14818_length_5534_cov_5.482387"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PLM68_07130"
     CDS             <1..1478
                     /locus_tag="PLM68_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT55167.1"
                     /translation="HLFKIQYQRNWSWDVGVPGGDAFPGPAKATYTDIGRHLLSASYR
                     ISNITEKWTALELKYFTQYIAREVMMEPNTVTQANLPNGNVQRTTPEVFYPNGYHLTN
                     GVQLQGTWDLSDKNTLIAGIDVWGRKLRTGREKYIRIDILNPAGNIIKTNHVVRGETP
                     IPESSFSSAGLFFQNETRFLENRLTMILGGRFDGIRIKNQEGYDVDYIIMNGERNDNP
                     PTQRITFEKGTEYNISWSANAGFLYKLSGSTDLSLNLARAFRAPSLEERFKYIDLGNF
                     VRLGDPALKPEKSYSADLGVRVWKSKFNLQAGVFINRLNNMIVEVPGEFIFRLTADSK
                     ADTIPALVNANVRKALLYGVDFKFAFNFSRNFVFFGSGAYVRGMALETDANLPLIPPL
                     NGRAGLRYTWQAAGSAEFSVLGAARQDKIAEGEKETAGYYRLDFTLNTKAFDLGFMKI
                     QGFAGIDNITDNRYTNHLSTNRGDISVEPGRNIFVRLRLSF"
     gene            1602..2186
                     /locus_tag="PLM68_07135"
     CDS             1602..2186
                     /locus_tag="PLM68_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT55168.1"
                     /translation="MAVKIRLSRHGKKNFAYFHIVVADSRSPRDGRLIETLGTYNPNT
                     DPATIQLDADKALYWLNNGAQPTLTARRLLSYKGVLLKKHLLEGVAKGALTQEQADAK
                     WEAWMQEKEAKVVAKKGKLEQESRAQMKKRLEAESSVNKERAAALLAKREEAAARLAA
                     AAAAAAAKAEETIAEIEGTAVPEAEEEKGQAATE"
     gene            2128..2715
                     /locus_tag="PLM68_07140"
     CDS             2128..2715
                     /locus_tag="PLM68_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55169.1"
                     /translation="MKEQQFLKQKKKKARQQQNNDMGIRERFLPLAMVRRTFGKGGEL
                     ILKFRPGVSQDILEQLGENEPVFIQVDGIPVPFYIASFDFRGNDQALVCFENYLSEDL
                     AAEWVGKTVQYKATGEEATEGGVLLVGYTFLATNARGNKFKGTVSGFFDYPGNPCLEL
                     EFEDGTRTLLPFHEDFIQKTDTKRKVVHLLLPEGL"
     gene            complement(2734..4290)
                     /locus_tag="PLM68_07145"
     CDS             complement(2734..4290)
                     /locus_tag="PLM68_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55170.1"
                     /translation="MKRIYAMLLMFFLGIFASSCTGLFGWKNYEGSEQFRDDVAVFAD
                     FAVQKQFITLYYLKATSDGFTHNILKGTVSNEEYDVFFGAVAQMAPYIKEYKSAFKNL
                     EKSGVLTSVNTKGILSSGGKFLSWVTGSGERSRERVLKVASNIPASDRTELYNKLRPE
                     WKAKTSSESDFWKKLEKGDFDYQAAQMFNDFQQDNEEFGTTSVERGLTIQKIFVAEGA
                     KGVEAGAELMVDALQTTTSGGLDAGDIGEMTLDIYNSNAEADPSQMAEEITAAVNSLG
                     PDDPTAKEMGSGYASKMASIMKEVVDVARTDTENVGGTNRSFVFVEDDDPQSQADIVI
                     AQNTQITSSSMASVYVTLGHIVDQGQKLIYTILNKGKWLISAVDPDGNKSTREVDVPA
                     GDVVIVTLTTTPPAKEGEEEEQPADYTDWNKIIAQYEFLKSYPPFSGACTFVGYSDNM
                     FYMAVTIRAKNSDAEKYRDKIGDFGFDDHSSGGNIMYWGPKYGNYYAHISFAASGTSG
                     YTDIMFKNLL"
     gene            complement(4348..4977)
                     /locus_tag="PLM68_07150"
     CDS             complement(4348..4977)
                     /locus_tag="PLM68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOT55171.1"
                     /translation="MKSTDNIVICLGRQMGSGGYIAGKMIASALGLFFYDKELLYYAA
                     KESGLCEEIFENADEKVTRGLSRALNAQDFLGGAFFDPMTAPLSRDRIFSIQSGVIRK
                     IASEQSCLIIGRCADYILRDHPRMRSVFIHAPLPLRIANIAERRGISPEEAEACIHKE
                     EKKRAAYYNYFTNKEWGKADSYHFSLDAGLLGLDNCARLAAHLIKESLD"
     gene            complement(4961..>5534)
                     /locus_tag="PLM68_07155"
     CDS             complement(4961..>5534)
                     /locus_tag="PLM68_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT55172.1"
                     /translation="RSFYQFGGDYAVGAYGIANRMVSFFVMIMFGFNQGMQPLAGFNY
                     GARQYKRVINIFKLTLLCSFCVSVLAFIIAQLFPEAAVSLFTRDETLARLTIQGLRID
                     LVAFPLVSLSMVTSSFFQSIRKPAKAIFLSLTRQVIFLIPSLLIFPRFFGVTGVWGSM
                     PVSDTAAFIVAAILISKQIKEFRKEYEING"
BASE COUNT         1464 a         1337 c         1359 g         1374 t
ORIGIN      
        1 accatttgtt taaaatccag taccagcgaa actggtcatg ggatgtaggc gttccgggag
       61 gcgatgcctt tccggggcct gcaaaagcaa cctatacaga tataggtcgt catttgttgt
      121 ctgccagtta cagaatatcc aacatcaccg aaaaatggac ggccctggaa ttgaagtatt
      181 tcacccaata cattgcccgg gaagtaatga tggagccaaa tacggtaacc caggccaacc
      241 tgcctaacgg gaacgtgcag cgcaccacac cggaagtatt ctatccgaat ggttatcacc
      301 tgaccaacgg ggtacagctg cagggtacct gggacctgtc cgataagaac acactgattg
      361 caggaattga tgtttggggc cgcaagctgc gtacaggcag ggaaaaatac atccggatag
      421 atatattgaa tccggcgggc aatattatca agaccaacca tgtggttcgc ggtgagacac
      481 ccatccccga atcaagcttc agcagtgcag gattgttttt ccagaatgaa acacgatttc
      541 tggagaaccg gctgaccatg atcctgggtg gccgctttga cggaatccgg atcaaaaacc
      601 aggaaggata tgatgtggat tacatcatca tgaacggaga gcggaatgac aatcccccca
      661 cccagcgtat cacatttgaa aaagggacgg aatataatat atcctggagt gccaacgcgg
      721 gctttcttta taaattgtca gggagtacag acctgtcgct aaatcttgcc cgggcattca
      781 gggcgccttc cctggaagag aggttcaagt atattgacct gggaaatttt gtccgcctgg
      841 gagatccggc cctgaagccg gaaaaatcct attcggcaga cctgggggtc agggtttgga
      901 aatcaaaatt caaccttcag gcaggcgttt tcatcaaccg gctgaacaat atgatcgtgg
      961 aggtgccggg agagttcatc ttcaggctta cggccgattc aaaggcagat accattcctg
     1021 cccttgtcaa tgccaatgta agaaaggccc tcctttacgg agtggatttt aaatttgcct
     1081 tcaatttcag ccggaatttc gtgtttttcg gctcgggggc atatgtccga gggatggccc
     1141 tggaaacaga tgccaacctg ccactgatcc ctccgttgaa cgggcgtgcc ggcctcaggt
     1201 atacctggca ggccgcagga tcggccgagt tttccgtgct cggcgcagcc cgccaggata
     1261 agattgccga aggagaaaaa gaaacggcag gatactacag gctggacttt accttgaata
     1321 caaaggcctt tgacctgggt ttcatgaaaa tacaaggttt tgcaggtatt gacaatataa
     1381 ccgataaccg ttacaccaac catctttcta ccaatcgtgg ggatataagc gtggaaccgg
     1441 gaaggaatat ttttgtacgc ctcaggcttt ctttctgatc cgggcttacc cgggaggtgt
     1501 tctaaaaatt ttgccatcac cacaaaaaca catatctttg cggacctttt ctcgggaaga
     1561 taaggaagtt aacacaattc attaacttta agatcagatt tatggcagta aaaattcgtc
     1621 tttcacgcca tggcaaaaag aactttgcct attttcacat tgtagttgcc gattcacgct
     1681 caccccgcga tggccgtttg attgaaaccc tgggaaccta caatcccaac accgatcccg
     1741 caaccataca gctggatgcg gataaagccc tttattggct caataacggt gcacagccca
     1801 cattgacagc ccgcaggctt ctttcctaca aaggagtgct gttaaaaaag catttacttg
     1861 aaggtgtcgc caaaggggct ttgacacagg agcaggcaga tgcaaaatgg gaagcctgga
     1921 tgcaggagaa agaagccaag gtcgttgcta agaaaggcaa actggaacaa gagtcacgcg
     1981 cgcaaatgaa aaaacgcctg gaagcagaat ccagcgttaa taaagagcgt gccgccgcat
     2041 tgcttgcaaa acgcgaagaa gccgccgccc ggttggccgc cgctgccgct gcagccgctg
     2101 caaaagcaga agagaccata gccgaaattg aaggaacagc agttcctgaa gcagaagaag
     2161 aaaaaggcca ggcagcaaca gaataatgac atgggaatca gggaaaggtt tctgccactg
     2221 gcaatggtga gaagaacctt cggaaaggga ggggagctga tcttaaagtt ccgccccggc
     2281 gtttcccagg acattctgga acaattaggt gaaaacgaac cggtattcat ccaggtggac
     2341 ggaataccgg ttccttttta tattgcatca tttgattttc gtggaaacga ccaggcattg
     2401 gtctgctttg agaattacct gtcagaagac ctggctgcag aatgggtggg aaagacggtg
     2461 cagtacaaag caacagggga agaagccaca gagggaggag ttcttctggt gggatatacg
     2521 ttcctggcta ctaatgcccg cgggaacaag ttcaaaggaa ccgtttccgg attctttgac
     2581 tatcccggga atccctgcct ggagcttgag ttcgaagacg gaacgcgaac cttattgcct
     2641 tttcatgaag acttcattca gaagaccgat acaaaaagaa aggttgttca cctgttattg
     2701 cctgaaggac tttgaccaac aggcccccct ctgctatagc aggttcttga acattatatc
     2761 tgtatagccc gaagttccgc tcgcagcaaa agaaatatga gcataatagt tgccatattt
     2821 cggtccccag tacattatat ttccgccgct gctgtgatca tcaaatccga aatcaccaat
     2881 tttgtccctg tatttttctg cgtcggaatt ttttgccctg atggtcacgg ccatataaaa
     2941 catattatca ctgtatccga caaaagtaca agccccggaa aaaggcgggt agcttttcag
     3001 aaactcatat tgtgctatga ttttattcca gtctgtataa tcggcgggtt gttcctcctc
     3061 ttccccttct ttcgcaggcg gtgtggtggt tagtgtaacg ataacaacat ctccggctgg
     3121 cacgtccact tcacgggtag acttgttccc gtccggatct acggccgaga tcagccattt
     3181 tcccttgttc agtatggtgt agatgagttt ctgtccctgg tcaactatat ggcccagtgt
     3241 aacataaaca gaggccatgg aagaggaagt gatttgtgtg ttctgggcta tcacaatatc
     3301 ggcctggctc tgcggatcat cgtcttccac aaatacaaag gaacggttgg tcccaccgac
     3361 attttccgta tccgtcctgg ctacatccac gacttctttc attatggagg ccatctttga
     3421 tgcgtagccc gagcccattt cttttgccgt tggatcgtcg ggccccaggg agttgacggc
     3481 agcggtgatc tcttctgcca tctgggaggg atcggcctct gcgttggaat tgtaaatatc
     3541 gagggtcatt tccccgatat caccggcgtc cagtcctccg gaagtggtgg tctggagggc
     3601 atcaaccatc agctcggccc ctgcttcaac accttttgct ccttcggcta caaagatctt
     3661 ctggatggta aggccccgct caaccgaagt ggtcccgaat tcttcgttat cctgttggaa
     3721 atcgttgaac atctgggctg cctggtaatc gaagtcacct ttttccagtt ttttccagaa
     3781 atcactttca ctggaggttt ttgctttcca ttcaggtctg agtttattgt aaagttctgt
     3841 ccggtccgat gcgggtatgt ttgaagccac tttcaataca cgttccctgc ttctctcccc
     3901 gctgccggta acccaggata ggaatttgcc tcctgaggaa aggatgcctt tggtgttcac
     3961 agaggttagg acacctgatt tttccaggtt cttaaaggcg cttttgtact cctttatata
     4021 aggagccatt tgggcaactg ctccaaagaa cacgtcgtat tcttcattgc tcaccgtccc
     4081 tttgaggata ttgtgggtga aaccgtcaga cgttgccttc aggtaataaa gtgtgatgaa
     4141 ttgtttttgt acggcaaaat cggcaaaaac cgcaacatca tcccggaatt gttctgatcc
     4201 ttcataattt ttccagccaa aaagaccggt acaactgctg gcgaaaattc ccaaaaagaa
     4261 catcaggagc atggcgtaaa ttcttttcat aatcagattg ttataatata cctctaaggt
     4321 agcagaaaat tattattctg caatacctca atccaggctt tctttgatca gatgggctgc
     4381 caaccgggca caattgtcaa ggcccagtaa accggcatca agggagaagt ggtatgaatc
     4441 ggccttgccc cattccttgt tggtgaaata attgtaatac gcggcccttt tcttttcttc
     4501 cttgtgaatg catgcttctg cctcttcggg agaaatgccc ctgcgttcag ctatgttggc
     4561 tatgcgcagg ggaaggggag cgtgtatgaa gacactgcgc atccggggat gatcccgcag
     4621 gatgtaatcg gcacaacggc caatgatcag gcaggattgt tcactcgcga tcttgcgtat
     4681 caccccggac tggatgctga atatcctgtc tcttgacaaa ggggccgtca tgggatcaaa
     4741 aaaggcacct cccaggaagt cctgtgcatt cagcgcacgc gataaacctc ttgtgacctt
     4801 ttcatcggca ttctcaaaga tctcttcaca aaggccactc tctttggctg cgtaatacag
     4861 aagttccttg tcgtaaaaga aaaggcccag ggccgaggca atcatttttc cggcaatata
     4921 accgccgctt cccatctggc ggcccaggca aatcacaatg ttatccgttg atttcatatt
     4981 ctttcctgaa ttcttttatt tgttttgata tgagtattgc agccactatg aatgcggctg
     5041 tgtcggatac gggcatactg ccccacaccc cggtgacacc aaaaaatcgt ggaaaaatga
     5101 gcagcgaagg gatcaggaat atcacctgac gggtaagaga aaggaaaatg gcctttgcgg
     5161 gtttgcgtat ggactggaaa aaactggaag tgaccatgga cagcgacacc agcggaaacg
     5221 ccaccagatc aatgcgcaat ccctgtatgg taaggcgggc cagtgtctcg tccctggtaa
     5281 acaggctaac ggcagcctcg gggaacaatt gtgcaatgat aaaggccagc acgcttacgc
     5341 aaaaagaaca gagcagcgta agtttaaaga tgttgatcac ccgtttgtat tgccgtgctc
     5401 cataattgaa tcctgccagc ggctgcattc cctggttaaa accaaacatg atcatcacaa
     5461 agaaggaaac catcctgttg gcaattccgt aggctccaac ggcataatcc cctccaaact
     5521 gatagaatga acgg
//
LOCUS       DAOSOF010000125         5514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_14879_length_5514_cov_4.619527, whole genome shotgun sequence.
ACCESSION   DAOSOF010000125 DAOSOF010000000
VERSION     DAOSOF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14879_length_5514_cov_4.619527"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="PLM68_07160"
     CDS             <1..1566
                     /locus_tag="PLM68_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT55173.1"
                     /translation="VKGADGYVVRYGIEPGKLYNSYMVYDKNFLTIHSLNTSPEYYYE
                     IEAFDSGLNYYKEISEVVNGTGAEIELTKIKPGERPAWGWGGADVPRLMIHQGQNDYV
                     FEDIEPGYNYTLRHSYGPVLWQEELTQKELIGDSTKPTVTVNLTELGVGTRTTGMLEL
                     RVYPGKEKGKIVVTTNYNRRMSFAPSPVVNEDNTVTFNLNAPNAKNVQINAQFAERQN
                     MDKNADGIWTITLGPVAADIYPYSFIVDGVTIMDPQNPEWFPNEKFKNSLVEVRGKEP
                     LNHEVTNVLHGSVDYTLYYSKTLGMYAPVMVYTPPCYDQSKKNYPVFYLISGTTDTEE
                     TFFKVGRVNFILDNLIAQGKAKEMIIVMPYGNPGFYLTEPKFMDYFSGDFTGDLMPFI
                     ESNYRTINDRDHRAIAGFSRGGGQSLRCGLGNFDKFSHIISYSSYIGTEEFEKNYRFL
                     YEDPARTNKMMKLYWLGVDKADFLYGGATEFMELMDKYGIQTVRLETEGLRGHTWMAV
                     KRFLNETLPLLFN"
     gene            1592..2758
                     /locus_tag="PLM68_07165"
     CDS             1592..2758
                     /locus_tag="PLM68_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT55174.1"
                     /translation="MKKIFLIIALSAFVLPGNVTYAQNPRRALTGRQLAAMFPPGVKS
                     PECNPDGTVTFRFQAENASVVELNCQMFDKNKAMTKDEKGLWSITVTPPAPDIYPYCF
                     VVDGIQVTDPNNVLIFPNENFKNSLVDVRGPAPDFQDMQDVPHGKISYRYYHSVILGF
                     DRPMCVYTPAGYDPKGKEKYPVLYLIHGMTDTYETWFRVGRINMILDNLIARGLAKKM
                     IVIMPYANPYPEMMRRGVARRQDVMGTDIFTKEILEEVIPFIEKNYRVRPDAGSRAIA
                     GFSLGGRQSLACGLGNPDTFHYVCAFAPAVFGDEVSTNFDNGIYAKPSVISSRLKLMW
                     LCCGTSDFLYQASLVLHQSLQERGIEHKTMFPGGGHTWMNCRDYITEVAQHLFK"
     gene            2765..4324
                     /locus_tag="PLM68_07170"
     CDS             2765..4324
                     /locus_tag="PLM68_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT55175.1"
                     /translation="MHKIVIVSLLLSLFLCGKLNAQEARIKIDTDRRIGQIDNNIYGN
                     FVEHLGRCVYGGIFDPASSKSDQDGLRTDVLDAIRGLSVSLTRYPGGNFVSNYHWLDG
                     VGPERTPRMELAWSHLETNEFGTNEFVAFARKVGTEPYFAVNLGTGSIVEAQSWVEYC
                     NVKEGPYYAELRRKHGYPEPHNIKYWGLGNEMDGDWQMGQLSAEDYVKKAREAAKLMR
                     STSPDIKLIASGSSNYSGGADPYHWNATILAGLKGYIDYIALHMYVGNYRDNYYDFLA
                     TPLQMDERTQIVKGMILREKSYRPVYIAWDEYNVWYRARSGDDVVGTRALEERYNLED
                     ALVITGFLNCFVRNADIVKMANMAQLVNVIAPIFTEGDDLFLQTIYYPLQMFSNNMKG
                     ESLDVFVDSPVYNTSPRFSLGDNEQSTYLKDVPYLDVTAALDGDQLVVCVLNRHKDNA
                     ISAEVILQQGAFTGPVEIYEINGPNIKSENDFGKTNVQTVRKNDLKKPGTSFTYPFPA
                     HSVTMFKGKIK"
     gene            4346..5509
                     /locus_tag="PLM68_07175"
     CDS             4346..5509
                     /locus_tag="PLM68_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT55176.1"
                     /translation="MKKTSLHSLMTLAAAVFMSVNSFAQVVEDFKPSSANQPGKEYPM
                     VNSEGRVRVQISAPEAKFVQLDIGAVKYDLVKDENGVWTGESAPQDEGFHYYQLNIDG
                     ASVPDPASLYYYGASRWGSGIEIPAHDQDFYALKNVPHGQVREVYYFSATNNTMRHCF
                     IYTPPGYETELSKRYPVLYLQHGGGENEYGWPSQGRTALIMDNLIADGKARPFIIVMD
                     NGSWTMDRPGQQRPAQGETWPPRNWAEGFMNTLVNDIIPMVDKSFRTLPDGMHRAMAG
                     LSMGGMQTKAITLAKPNVFSYIGIFSGGSITVEDVQNTPGFRENNKLVFVSFGSRELE
                     NRRPGFGGDPREETEKLKASGMNAHFYVSPDTAHEWQSWRRSLYQFAPLLFKD"
BASE COUNT         1522 a         1294 c         1357 g         1341 t
ORIGIN      
        1 gtcaaagggg ctgacgggta tgtggtgcgt tacggcatag agcctggaaa gctatacaac
       61 agttatatgg tttacgacaa gaatttcctt actatccaca gtctgaatac atcccctgaa
      121 tattattacg aaatagaggc atttgactcg ggccttaatt actataagga aatatctgag
      181 gtagtaaacg gtaccggtgc tgaaatcgaa cttaccaaaa tcaagcccgg tgaacgccct
      241 gcctggggat ggggaggagc agacgtcccg cgtctgatga ttcatcaggg gcagaatgat
      301 tatgtgtttg aagacataga gccgggttat aattatacac tcagacattc ttacggtcct
      361 gttctctggc aggaagaatt gactcaaaag gaactcatcg gggacagcac caaacctacg
      421 gtaaccgtca atttgacaga acttggcgta ggaacccgaa caacaggcat gcttgaactc
      481 agggtttatc cgggaaagga aaaaggaaag atcgtggtca ctaccaatta caaccggaga
      541 atgtcctttg ccccgtcccc tgttgtgaat gaagataaca ccgtaacctt caatctgaat
      601 gcacctaatg caaagaacgt tcagatcaat gcccagtttg ccgagagaca gaacatggac
      661 aaaaatgcgg acgggatatg gactatcaca ctgggccctg tggcagctga tatctatcct
      721 tacagtttca tagttgacgg ggttaccatc atggatcctc agaatcctga atggttcccc
      781 aatgaaaaat tcaagaacag cctggtagaa gtaaggggga aagagcctct gaaccatgaa
      841 gttactaacg tgcttcacgg aagcgttgac tatacccttt attattcaaa aacgcttggg
      901 atgtatgcac ctgtaatggt ttatacacct ccgtgctacg atcagtcaaa gaagaattat
      961 cctgtattct atcttatcag cggtacaacg gatacagagg aaacattctt caaggtcgga
     1021 cgggtcaact tcatcctgga taacctgatt gcacagggaa aagcaaagga gatgatcata
     1081 gtcatgccct acggcaatcc cggcttttac cttaccgagc cgaaattcat ggactacttt
     1141 tccggggatt tcacgggtga cctcatgcct ttcatagaga gcaattatcg tacaatcaac
     1201 gatcgtgacc atcgtgccat cgccggtttt tcccggggtg gtggacagtc acttcgctgt
     1261 ggcctcggca attttgacaa attttcccat atcatttcct acagctctta cattggcacc
     1321 gaagagtttg aaaaaaacta caggttccta tatgaggatc ccgccaggac caacaaaatg
     1381 atgaaattgt actggctggg agtcgataag gcagatttcc tgtacggagg tgcaaccgaa
     1441 ttcatggaac ttatggacaa atacggaatt caaacggtcc ggctcgagac ggaaggcctg
     1501 cgcggacaca cgtggatggc tgtgaaacgt tttctcaatg aaacccttcc cctgctgttt
     1561 aattaaagga caataccaaa tgaacaatca tatgaaaaag atatttctga tcattgcttt
     1621 atcggcgttt gtattgcccg gtaatgtaac gtatgcccag aacccccgcc gcgcccttac
     1681 cggcaggcaa ctggccgcca tgtttccccc gggggtgaag tctcccgaat gcaatccgga
     1741 tggaacagtt acgttccgtt tccaggcaga aaatgcttct gttgtcgaac tcaattgcca
     1801 gatgtttgac aaaaacaaag ccatgacaaa ggatgaaaaa ggattatgga gcatcactgt
     1861 aactccaccg gcccccgaca tatatcctta ttgctttgtg gtagacggga tccaggtcac
     1921 agaccccaac aatgtgctta ttttcccaaa cgaaaatttc aagaatagtc ttgtggacgt
     1981 ccgcggaccg gccccggact tccaggacat gcaggatgta ccgcacggaa agatatccta
     2041 cagatactat cattccgtga ttctgggttt tgacaggccc atgtgtgtat acacccctgc
     2101 cgggtatgac cccaaaggca aagagaagta tcctgttctt tacctgatac atggcatgac
     2161 cgatacctat gagacctggt tcagggtggg caggatcaat atgatcctgg acaatctgat
     2221 cgcccgggga ctggcaaaaa aaatgatagt tatcatgccc tatgcaaatc cgtatcccga
     2281 gatgatgcgc cgtggagttg cccggagaca ggatgtgatg ggcaccgata tattcaccaa
     2341 agaaatactg gaggaggtta tcccgtttat agaaaagaac taccgggtca ggccggatgc
     2401 cggcagcagg gccattgccg gtttctcgct gggtgggaga cagtctcttg cctgcggtct
     2461 gggcaacccg gatacattcc attatgtatg cgcctttgcg cctgccgttt ttggtgacga
     2521 agtatcaacg aactttgata atggcattta cgcgaaacct tcagtcatca gctccaggct
     2581 caaactcatg tggttgtgtt gcgggacctc cgatttcctg taccaggcat cgctggttct
     2641 gcaccagtcg ctccaggagc gtggcattga gcacaagacc atgttccccg gaggtggcca
     2701 tacctggatg aattgccggg attacataac cgaagtagca caacatcttt ttaaataatt
     2761 gaatatgcat aaaattgtaa ttgtttcatt gttgttatca ttattcctat gtgggaagct
     2821 gaatgcccag gaggccagga taaagattga cacggaccgc aggataggtc agattgacaa
     2881 caacatatac ggcaactttg tggagcatct tggaaggtgc gtgtacggtg gcattttcga
     2941 tcctgcctct tccaagtcag atcaggacgg tttgcgtacc gacgtacttg atgcgatcag
     3001 gggcctgagc gtttccttaa cccgttaccc cggaggaaac tttgtatcga attatcattg
     3061 gcttgatggt gttgggcccg agaggacccc acgcatggaa cttgcctggt cgcacctgga
     3121 aaccaatgaa ttcggcacga acgaattcgt ggcctttgca agaaaagtag ggaccgagcc
     3181 ctattttgcc gtcaacctgg gaacgggatc aatagtagag gcacagtcat gggtagagta
     3241 ctgtaatgtc aaagaaggcc cttattatgc cgagcttcgc aggaaacacg gttatccgga
     3301 acctcacaac atcaagtatt ggggtcttgg caatgagatg gacggtgact ggcaaatggg
     3361 tcagcttagc gccgaagatt acgtgaaaaa ggccagggag gccgctaagc ttatgaggag
     3421 cacttctccc gatatcaagc tgattgcctc gggttcttcc aactattcgg gcggagccga
     3481 tccctaccat tggaatgcga cgattcttgc cggccttaaa ggctatatag attacatcgc
     3541 acttcacatg tatgtgggaa attaccggga caactactac gatttcctgg ccaccccgct
     3601 ccagatggat gagcgtaccc agatagtcaa gggaatgata ttgagggaaa agtcgtatcg
     3661 tcccgtgtat attgcctggg atgaatacaa tgtctggtac agggcacgca gtggcgatga
     3721 cgttgtagga acacgggctc tcgaggaaag atacaacctt gaggatgcct tggtgatcac
     3781 gggattcctg aattgtttcg tcaggaacgc cgacattgtc aaaatggcca atatggctca
     3841 gctggtgaat gtgatagctc ccatatttac ggaaggtgac gatctcttcc ttcagaccat
     3901 atattatccc cttcagatgt tctccaacaa catgaaaggc gaatctctgg atgtattcgt
     3961 ggatagtccc gtgtacaaca cttcgccaag gttttcattg ggagacaacg aacagagcac
     4021 ttacctcaag gatgttccct accttgacgt gacggctgcc ctggacggtg accagttggt
     4081 ggtatgtgtt ctcaaccgcc ataaggataa cgccatttcc gctgaagtca tattacagca
     4141 aggcgcattc acaggtccgg tcgaaattta tgagatcaac ggtcctaata tcaaatcaga
     4201 gaatgatttc ggcaaaacaa atgtgcaaac cgtaaggaaa aatgacctga agaagcctgg
     4261 tacttccttc acgtacccgt tccctgcaca ttctgttacc atgttcaaag gcaagattaa
     4321 atgacaactc attaaaatac ttattatgaa aaaaacatct cttcattcat tgatgaccct
     4381 ggcagcagca gttttcatgt cggtcaactc cttcgcccag gtagtcgagg atttcaagcc
     4441 ttccagtgca aaccagcctg gaaaagagta tcccatggta aactcggaag gacgggtgcg
     4501 cgtacaaatt tccgcaccgg aagccaagtt tgtgcaactt gacatcggtg ccgtcaagta
     4561 cgatcttgtt aaggacgaaa acggggtgtg gacaggcgag tcagctccgc aagacgaagg
     4621 attccactat taccagttga atattgacgg cgcatctgtg cccgatcctg caagccttta
     4681 ttactatggg gcaagccggt ggggtagcgg gattgagatc ccggctcacg accaggactt
     4741 ctatgcactg aaaaatgtcc ctcatggaca ggtacgcgag gtttattact tttccgctac
     4801 aaacaatacc atgcgacatt gtttcatata cactcccccc ggatatgaaa cagaactttc
     4861 aaaacgttac cctgtactct atctgcagca cggtggaggt gaaaatgagt atggctggcc
     4921 ttcacaaggc aggaccgctc ttatcatgga caaccttatc gccgatggca aggcccgtcc
     4981 attcatcata gtcatggaca acggcagctg gaccatggac agaccaggcc agcaacgtcc
     5041 tgcccaggga gaaacatggc ctcccagaaa ttgggcggaa ggtttcatga ataccctggt
     5101 caatgatatt attcccatgg tggataagag ttttcgcaca ttgccggacg gcatgcatcg
     5161 tgctatggca ggtctttcca tgggagggat gcagacaaaa gccatcaccc tggcaaagcc
     5221 taacgtgttc tcatacatag gtattttcag cggtggttcc ataaccgttg aagatgtaca
     5281 aaatacaccc ggttttcgcg aaaacaacaa actagtattc gtaagtttcg gaagccgtga
     5341 attggaaaac cgcaggcccg gctttggtgg ggatccaagg gaagaaaccg aaaaactgaa
     5401 agcatccgga atgaatgctc atttttatgt ttcaccggac acggcacacg agtggcagag
     5461 ctggagaaga agcctgtatc aatttgcacc actgttattt aaagattaat tttt
//
LOCUS       DAOSOF010000126         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_15319_length_5385_cov_4.910131, whole genome shotgun sequence.
ACCESSION   DAOSOF010000126 DAOSOF010000000
VERSION     DAOSOF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_5385_cov_4.910131"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLM68_07180"
     CDS             complement(<1..1038)
                     /locus_tag="PLM68_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOT55177.1"
                     /translation="MKRVNLFYLLTIFLLFASCERCIIPGGGYEHNLLIYIAADNNLS
                     EVARENMNDIITHASVPAGHALVVFTDIKNQGAFLIGVSTRNKVIKLDTLKRYGAVNS
                     MLPEVMGQAIEDAREEYPAKTYGMLFWSHGTGWLPRGLYGEVPQYPVKPTSVASPFEY
                     DIYDPAYPRTKTFGQDGASEMEIPDMVTVLQKYHHEYICFDACLMADMQTYYQLREVC
                     DYIMGSPAEIIDTGYPYNKLTSVLFPYEGLSSLTALCDAYFEKYNSQTGYNRSGTISL
                     VKTEHIQSLAQAFRTLIRNGGIDPEDVDRTGLQTYDRLTEHVFWDIDQMALMLGNTSD
                     YAHFRTELDKTV"
     gene            1103..1297
                     /locus_tag="PLM68_07185"
     CDS             1103..1297
                     /locus_tag="PLM68_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOT55178.1"
                     /translation="MKSSHVLALLGGALIGATLALLFAPAKGSETREKIKETVDKGVD
                     EIKKECHKRFPKNQEEGLEA"
     gene            1309..1659
                     /locus_tag="PLM68_07190"
     CDS             1309..1659
                     /locus_tag="PLM68_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55179.1"
                     /translation="MNIKDSVKEYLGLKSDAFKLGLVENLSLVINQFLSTMILLLVLL
                     VSLVFLATGANKWLGMVLDNDIAAPLMTGGFFLVLFLILFLLRKKLFLNKFVRLFARM
                     FFEEKDPNTGRNDL"
     gene            1656..1919
                     /locus_tag="PLM68_07195"
     CDS             1656..1919
                     /locus_tag="PLM68_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55180.1"
                     /translation="MRSRKAIYSNIRTIEQLRAERNSLEWHIKGVEDRIRDSIPSGFA
                     NGKFFPSEGSGFLSWLPLLIPVTITAFTLARHLNRCVQKIRNH"
     gene            1936..3930
                     /locus_tag="PLM68_07200"
     CDS             1936..3930
                     /locus_tag="PLM68_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT55181.1"
                     /translation="MKIAFACLAGIAGLSCTQGPKETKKQEPEFRYLAEEFADIKIIR
                     YKVPGWDDLSLQQKTFIYYMGEAAKCGRDIFWDQNFKHNLEVRKCLETILETYPGDRE
                     IHDFQEFLVYAKRVFFSNGIHHHYAEEKILPGCTAVYMRHLLEESDIDADIDFLVDIM
                     CDPELYAMRKSSGREGDLLLTSSTNFYAGVNRARAEAFYAAMEDPGNLRPVEYGLNSR
                     LSEKDGVVFEEPYKADGLYGPAIQQICHWLEKAAGVAESPEQASYIKTLIDYYQTGDL
                     ALWDAFNVAWVQDTDTFVDFINGFIEVYGDPLGRKGSWEGNVNFMDTEASRRTTIISQ
                     NAQWFEDNSPVDPRFRKEQVTGVSAKVINVAMLGGDSYPSTPIGINLPNSDWIRKEYG
                     SKSVTIANITNAYDKAAQESPKNMLDEFSWDEQEVALVKQFGTITGDLHTDLHECLGH
                     GSGQLLPGVSSNALGEYSSTLEEARADLFALYYLADPKLAELEILTDPEAYKAEYINY
                     IRNGLFTQLVRIEPGRNITQAHMQCRQLIASWALQNGNAIEKKVRDGKTYFVINDFGE
                     LRQLFATLLAQMQKIKSEGDYMAGKALVEQYAIQVDQELHREVLERYAALELKPYGGF
                     LNPDIVPVYNRKGEIRDLKLEYAEDFLQQQLSYGKAYNFL"
     gene            complement(3851..5167)
                     /locus_tag="PLM68_07205"
     CDS             complement(3851..5167)
                     /locus_tag="PLM68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT55182.1"
                     /translation="MKYAMNFFPLREVKRFDLARMPRPVSWYLRPIAWLLSFPAVWRR
                     HTKINKVNMEGIKTPYLLLCNHNAFYDFMVATVATFPHQAFYVVAVDGFIGREWLMRR
                     IGCIGKRKFTNDIALIRHLKTVVDRGKIAAIYPEARYSLCGTKAVLPESLGKLVKRLD
                     VPVVTLITYGHHINAPFWNTRTRGVRTKATMTRIITREEVSTMSVEQINRKINQAFEY
                     DDFAWQRENKIPVLYKDRARGLHKVLYQCPACRTEYRMDSGGAAIWCNHCSKRWTMSE
                     YGELKALEGETHFAHIPDWYEWERKQVRREVEEGRYCFQGNATVRMLPNSKGFIDFGK
                     AVLTHNMDGFTLEGNHDGTSYTLNIPAQSVYSCHIEYNYGKYKRDCVDLNTLNDSYWV
                     FPEGDDFSVTKISLATEELFIIKNYKLSRRIIAVAGNPQHIPVSGL"
BASE COUNT         1468 a         1246 c         1285 g         1386 t
ORIGIN      
        1 caccgtcttg tcgagctctg tcctgaaatg ggcataatca gacgtattgc ccagcatcaa
       61 ggccatctgg tcaatatccc agaaaacatg ctctgtgagc cggtcatatg tttgcagacc
      121 ggttctgtcc acatcctccg gatcaatgcc tccgttcctg atcagggtcc tgaatgcttg
      181 tgccagtgac tggatgtgct cggtcttaac cagggatatg gtaccggagc ggttatatcc
      241 tgtctgggaa ttgtattttt caaagtatgc atcgcataat gcggtcagac tgcttaaacc
      301 ttcgtaggga aacaacacgg aagtcagctt gttgtaaggg tatcccgtat ctatgatttc
      361 tgcaggagat cccatgatat aatcacaaac ttcccggagc tggtaatatg tctgcatgtc
      421 tgccatcaga catgcatcaa aacagatgta ctcgtgatga tacttctgta aaaccgtaac
      481 catatcgggg atctccatct ctgaagcacc atcctgcccg aaagtctttg tccgggggta
      541 agccggatcg tagatatcgt attcaaaagg cgaagctacc gatgtgggtt ttacggggta
      601 ttgcgggact tctccgtaca atccgcgggg cagccagcct gtgccgtgtg accaaaacag
      661 cattccgtag gtttttgccg ggtattcctc cctggcatct tctatggcct gtcccattac
      721 ctcggggagc atcgagttga cggctccgta ccttttcaag gtatctagtt tgatcacttt
      781 attacgcgta ctgacaccaa tcagaaaggc accctgattc tttatatctg tgaagaccac
      841 cagggcatgg ccggcaggga cagatgcatg ggtaatgata tcattcatat tttcccgggc
      901 aacttctgag aggttgttat cagctgcaat gtatatcagt aggttatgct cgtacccgcc
      961 tcccgggatt atgcaccttt cacatgaagc gaacaacagg aagatggtta acaggtaaaa
     1021 aagattaacc cttttcatat cgtaaagata atcctttttt attacctttg taagaagaca
     1081 taacatcaaa tattcctata ttatgaaaag ctcacatgta ctcgcattat tgggcggagc
     1141 cctgatcggg gctacgctgg cattgttgtt tgctcctgca aaaggaagcg aaacaaggga
     1201 aaagataaaa gaaacggtag acaagggtgt tgatgagatt aaaaaagaat gccataaacg
     1261 tttcccgaaa aaccaggaag aaggcctgga agcctaaaaa aaaacgcgat gaatattaaa
     1321 gactcggtta aggaatacct tggcctgaaa tcagatgcgt ttaagttggg tttggtggaa
     1381 aacctgagtc tggtcataaa ccagttcctg agtactatga ttttgttgct tgtacttctg
     1441 gtttcactgg tttttcttgc cacaggagca aacaagtggc tgggtatggt tctcgacaat
     1501 gacatagcgg ctcctctcat gacaggtggt tttttcctgg tcttgttcct gatcctgttt
     1561 ctgctgagaa agaagttgtt cctgaacaag tttgtcaggt tgttcgcaag aatgttcttt
     1621 gaagagaagg atcctaatac cgggaggaat gacctatgag aagcagaaaa gccatttact
     1681 ccaacataag gaccattgaa cagctcaggg cagagcgcaa ttcgctggag tggcacataa
     1741 agggagtgga agaccgtata cgtgatagca taccctccgg ttttgctaat ggtaagtttt
     1801 tcccttctga gggcagcggt tttctttcct ggcttcccct gcttattcct gtgaccataa
     1861 ccgcttttac actggcacgt catctgaacc ggtgcgttca aaaaatccgt aaccattagc
     1921 aaaacaatga aaataatgaa aattgctttt gcctgcctgg caggcatagc gggactatcc
     1981 tgtacccagg gtccgaaaga gactaaaaaa caggagcctg aattcagata cctggcagaa
     2041 gaatttgcag atatcaagat aatcaggtac aaggtgcccg gttgggacga cctgagtctg
     2101 cagcagaaga cattcatcta ctatatggga gaagccgcaa aatgtggcag ggatatattc
     2161 tgggaccaaa atttcaaaca caacctggaa gtgagaaaat gcctggaaac cattcttgaa
     2221 acatatcccg gagacaggga aatccatgat ttccaggaat tccttgttta tgccaagcgg
     2281 gtttttttca gcaatggcat tcaccatcat tatgcagaag aaaaaatcct tcccggctgc
     2341 acggccgttt atatgaggca tttgctggag gagtccgata tcgatgcaga cattgacttc
     2401 ctggtggaca tcatgtgtga tccggaactg tatgcaatgc gtaaaagcag cggaagggaa
     2461 ggtgatttgc tattgacatc atcgaccaat ttttacgcag gtgtcaaccg ggcccgggcc
     2521 gaagcatttt atgcagccat ggaagatccc ggcaacctgc gtcccgttga atacggtctg
     2581 aacagccgtc tatcagaaaa agacggagtg gtctttgagg aaccctacaa agccgacggg
     2641 ctgtacggac ctgccatcca gcagatctgc cactggctgg agaaagctgc cggagtggcc
     2701 gaaagccccg aacaagcctc ttacataaaa acgctgattg attattatca aacaggagac
     2761 ctggcccttt gggatgcctt caatgtggca tgggtgcagg ataccgatac ttttgtagat
     2821 ttcatcaacg gatttattga agtgtatgga gatcccctgg gaagaaaagg ttcctgggaa
     2881 ggaaacgtga attttatgga cacagaggct tcccgcagaa cgaccatcat cagtcaaaat
     2941 gcgcaatggt ttgaagacaa ttcccctgtc gatccccggt tcagaaaaga acaggtgacg
     3001 ggagtctcgg ccaaagtgat caacgtggcc atgctgggag gcgattccta cccttccaca
     3061 cccattggca tcaacctgcc caactcggac tggataagaa aggaatacgg atctaaatcc
     3121 gttaccattg ctaatatcac gaatgcctat gacaaagcgg cgcaggaatc accaaaaaac
     3181 atgcttgacg agttctcctg ggatgagcag gaagtggctc ttgtaaagca attcggaaca
     3241 ataacaggag atttgcacac agacctgcat gaatgcctgg ggcacgggtc gggccagctg
     3301 ttgccgggtg tgtcttccaa tgcactggga gaatacagtt ctacactcga agaagcgcgg
     3361 gcagatcttt ttgccttgta ttaccttgct gatccaaaac ttgcagaact ggaaattctg
     3421 accgatcctg aagcttataa agctgaatat atcaattaca tccgcaatgg cctgttcacc
     3481 cagctggtac gtatagaacc aggaaggaac atcacacaag cgcacatgca atgccgccaa
     3541 ctgatcgcat catgggcact tcaaaacggc aatgccattg aaaaaaaggt ccgggatgga
     3601 aaaacttact ttgtgatcaa tgatttcgga gaacttcgcc agctgtttgc cacgttgctt
     3661 gcccaaatgc aaaagatcaa atcagaaggg gattacatgg cgggcaaagc cctggttgaa
     3721 caatacgcca ttcaggtaga ccaggagttg cacagggaag tgctggaacg ttacgccgcc
     3781 ctggaactga agccttacgg gggtttcctc aatcccgata ttgttccggt atataacagg
     3841 aaaggtgaga tcagagacct gaaactggaa tatgctgagg atttcctgca acagcaatta
     3901 tcctacggga aagcttataa ttttttataa tgaataattc ctcggtggcc agggatatct
     3961 tggtcaccga gaaatcatca ccttccggaa acacccaata agagtcgttc agggtattga
     4021 ggtcgacaca atctcttttg tatttgccat agttgtattc tatatggcag gaatagaccg
     4081 attgggcagg tatgttcagg gtataactgg taccgtcatg gtttccctcc agggtaaaac
     4141 catccatatt gtgggtcagt acagccttcc caaaatcaat aaaacctttt gaatttggaa
     4201 gcatcctgac cgtcgcgttc ccttgaaaac agtaacgtcc ttcctcaact tccctgcgta
     4261 cctgtttcct ttcccattca taccaatcgg gtatatgggc aaaatgagtc tccccttcca
     4321 gggccttcaa ctccccgtat tcagacatgg tccagcgctt gctgcaatga ttgcaccaga
     4381 tcgcggctcc tcctgaatcc atcctgtact ctgtcctgca agcgggacat tgatacagca
     4441 ctttgtgcaa tcctcgggcc ctgtccttat aaagtaccgg tatcttgttt tcacgttgcc
     4501 aggcaaaatc atcgtattcg aaagcttgat ttatcttccg gttaatttgt tctacagaca
     4561 tcgttgaaac ctcctcccgg gtgatgatgc gtgtcatggt tgccttggtc cttaccccgc
     4621 gtgtcctggt gttccagaat ggagcattga tgtggtgacc gtaggtaata agggttacaa
     4681 caggtacatc aagacgtttc accagtttgc ccagtgactc cggcaaaacc gccttggtgc
     4741 cgcacaggga ataacgtgcc tcgggatata tggcggctat ctttcccctg tccacaactg
     4801 tctttaaatg gcgtatcagg gctatatcgt tggtgaattt gcgtttgcct atgcatccta
     4861 tgcgcctcat aagccattcc ctgccaatga atccgtctac agccaccaca tagaatgcct
     4921 ggtggggaaa ggtggcaacg gtagccacca tgaaatcata aaaggcattg tggttgcata
     4981 acaataaata gggagtcttt atgccttcca tgttaacttt attgatcttt gtgtggcggc
     5041 gccagacagc aggaaaactc agcaaccatg caatgggccg taaataccag cttaccggcc
     5101 tgggcatcct ggcaaggtca aaacgtttga cctcgcgcaa ggggaaaaaa ttcatggcat
     5161 atttcatgac cgcaaatata tacgatattt ttaacttgaa attttcggga aggaagtcgc
     5221 cttagtcacg aaaaatagaa aggggagctt ccaaaaagct cccctttctt ttgatgcagc
     5281 gaccttataa ggggggacct tgctgcaaat tacaggtcca cctgttcatt ggttttattt
     5341 taaaccggta cgggtgtcaa accacatttg ctttccccag aaatc
//
LOCUS       DAOSOF010000127         5335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_15508_length_5335_cov_4.451705, whole genome shotgun sequence.
ACCESSION   DAOSOF010000127 DAOSOF010000000
VERSION     DAOSOF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15508_length_5335_cov_4.451705"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLM68_07210"
     CDS             complement(<1..765)
                     /locus_tag="PLM68_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HOT55183.1"
                     /translation="MQLLDWIVIGLFFLVLVGIVIWVLSKKSKDSKDYFLGGRDVTWL
                     AIGASIFASNIGSEHLIGLAGAGASSGMAMAHWEIQGWIILLLGWVFVPFYSRSMVLT
                     MPEFLEKRYNKESRTILSFISLVSYVLTKVAVTVYAGGLVFQQVFGIEELWGIDFFWI
                     SAIGLVLFTALYTVLGGMKSILYTSVLQTPIILIGSLVILVLGFRAVGGWEEVLAVCG
                     ATPVNEYGDTMVNLIRNNSDPDFPWLGALLGSSIIGF"
     gene            complement(776..1921)
                     /locus_tag="PLM68_07215"
     CDS             complement(776..1921)
                     /locus_tag="PLM68_07215"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOT55184.1"
                     /translation="MKRILVTGCVTLVCMFIFCACSQTPSSSVSGLYPNDFKAEKDGQ
                     QTGLYILKNSLGMEVCITNFGGRVVSIMVPDREDKLRDVVLGFSNATDYMEIPSDFGA
                     SIGRYANRIGQGLFTLDSTDYQLPKNNFGHCLHGGPKGWQYKIYEVEKVSDNSITLVR
                     NSPDGDESFPGNVTAKVTFTVTTDNALEIAYQATTDKPTVINMTNHSYFNLSGDAANS
                     ILDHVLYVNAQYFTPVGRTLIPTGEIVPVAGTPMDFTIPKVIGKDIDSTGYEQIRFGN
                     GYDHNWILDTKGDMGAVAATLFSPESGIKLDVYTDEPGIQVYSGNFLDGKVTGKNGIV
                     YQKRAAICLETQHYPDSPNKPEWPTTVLRPGETYTSHCTYKFSTITD"
     gene            complement(1950..3881)
                     /locus_tag="PLM68_07220"
     CDS             complement(1950..3881)
                     /locus_tag="PLM68_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT55185.1"
                     /translation="MIKLWIIPFLSLVLHVYPIDPVPFTSVKVTDAFWGQRLKASREV
                     TIPLAFSKCEETGRYDNFVKAAHPGLENEVTGFSFDDTDVYKTIEGASYLLQTYPDEK
                     LEIYIDSVLALVAAAQEPDGYLYTARTMNPQQPHEWAGSKRWEKVEELSHEFYNLGHM
                     VEGAIAHFQATGKRTFLDIAIRYADCVCSEIGEGPGQVVAVPGHQIAEMALAKLYLVT
                     GDEKYLKQAKFFLDKRGYTSRKDLYSQAHIPVIQQGEAVGHSVRATYMYSGMADVAAL
                     TGDKSYIAAIDRIWENIVTKKLYITGGIGATHQGEAFGENYELPNMTAYCETCAAIGN
                     VYVNHRLFLLHGKAGYYDVLERTLYNGLISGVSLDGGSFFYPNPLESAGQHQRQPWFG
                     CACCPSNIARFIPSLPGYIYAVKDNSLYVNLFISNTLTTKILDNDIVLRQQTDYPWDG
                     DITITIDKAPGNNVIIKVRIPGWVQNQPVPGDLYSYADGKTPAYSIKVNGKTADGTLC
                     DGYFCMERKWKKDDSIEVAFQMEARTVTAKEEVEANRGRLAVERGPVVYCAEWPDNDF
                     DLQSLLLPPAPEFKAVPVPDKLYGIVQLETPARVQAAGDDYIPVAKEVLLTLIPYYAW
                     AHRGAGSMMVWLPRDKVSQ"
     gene            4031..>5335
                     /gene="araA"
                     /locus_tag="PLM68_07225"
     CDS             4031..>5335
                     /gene="araA"
                     /locus_tag="PLM68_07225"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOT55186.1"
                     /translation="MAFENFEVWFVTGAQLLYGGDAVIAVDAHSTQMVQGLNDSGNLP
                     VKVVYKGTVNSEKEVTATFKEANNCEKCIGVITWMHTFSPAKMWIHGLQALDKPLLHF
                     HTQFNKEIPWDTMDMDFMNLNQSAHGDREFGHMVTRMRKNRKVVAGHWQDEKAQAQIA
                     VWMRVATAWADAHDMRIIRFGDQMNNVAVTDGDKVEAEMRLGYHVDYCPIAELVAVQS
                     QVTDAEVDALVAEYEMQYKVAENCQAGGKDRAQVLRAAREEIALRRFLQEKGAKAFTT
                     NFDDLAGLDQLPGLACQRLMAEGYGFGAEGDWKTAALTRTVWFMSQDMPKGCSFMEDY
                     TIHFDGKKSAILQAHMLEVSPLLADKKPRLEVHPLGIGGKNDPARLVFTSKEGEGIAA
                     TVIDMGNRFRLIVNKVDCIRSKSLPKLPVASTLWIPRPDFEVG"
BASE COUNT         1353 a         1304 c         1304 g         1374 t
ORIGIN      
        1 aaacccgatg atggatgaac ccagcaaggc acccagccag gggaaatcgg gatcgctatt
       61 gttccgtata agattaacca tggtatctcc gtattcattt acgggggttg ccccacagac
      121 agccagcacc tcttcccagc ctcctacagc tctgaatccc agcacaagga tgaccaggga
      181 ccctataagg atgatgggag tttgcaggac cgaagtgtac agtatggatt tcatgccgcc
      241 cagcaccgtg tacagggcag tgaacaagac caggccaata gcagagatcc agaaaaaatc
      301 tatgccccag agttcctcta tgccgaatac ctgttggaaa acaagtcctc ccgcataaac
      361 agtaacagcc actttagtaa gtacataact aaccagggag ataaaagaca gtatggtacg
      421 tgattcctta ttataacgtt tttccaggaa ctcgggcatg gtaaggacca tgcttcgtga
      481 atagaaagga acaaaaaccc agcctaacag gaggattatc catccctgga tctcccagtg
      541 tgccatagcc ataccgctgg aagctccggc tcctgccaga ccaatcaggt gttcagaccc
      601 tatattggaa gcaaaaatag aagcccctat ggccagccag gtcacatcgc ggccacccag
      661 aaaataatct ttagaatctt tcgatttctt gcttaacacc catattacga tccctaccaa
      721 taccaggaaa aaaagtccga taacaatcca atccagtaat tgcataaaaa aacgattaat
      781 cggttattgt actgaattta taagtgcagt gactggtata ggtctctccc ggacgcaata
      841 ccgtggtagg ccattcaggc ttattgggag aatcgggata atgctgtgtt tcaagacata
      901 tagcagcacg tttttgataa acgatcccgt tttttcccgt aacctttcca tccaggaagt
      961 tgcccgaata gacctgtatc cccggctcgt ctgtatagac atccagtttt ataccgctct
     1021 ccggggagaa aagagtggcg gccacggcgc ccatgtcacc tttggtgtct agtatccaat
     1081 tatgatcata cccgttaccg aacctgatct gctcgtaccc tgtactgtca atatctttcc
     1141 ctatgacctt tggtatggtg aaatccattg gggtacctgc taccggcact atctcaccgg
     1201 tgggtatgag tgttcttcca acgggtgtaa agtattgagc attcacatac agaacatgat
     1261 caagaatact gttcgccgcg tctccgctca ggttgaaata cgaatggttt gtcatgttga
     1321 tgaccgtagg tttgtctgtg gtcgcctggt atgcgatctc cagtgcattg tccgttgtga
     1381 cggtgaatgt aactttggcc gtaacgtttc cggggaaaga ttcgtccccg tcaggcgagt
     1441 tccgcacaag tgtaatggaa ttgtctgaga ccttttccac ttcatatatc ttgtattgcc
     1501 atcctttcgg gccgccgtgc aggcaatgtc cgaaattgtt tttggggagt tgataatctg
     1561 tgctatccag agtaaaaagt ccctggccta tcctgtttgc atacctgccg atggaggccc
     1621 cgaaatccga gggaatttcc atataatcgg tcgcattgct gaagcccagg accacgtcac
     1681 gcagcttgtc ttccctgtcg ggaaccatta tggatacaac acgtcctccg aaattcgtga
     1741 tacacacttc catgcccagg ctgtttttaa gtatatacag tccggtctgc tgcccgtctt
     1801 tttctgcttt gaagtcgttg ggatacaatc ccgaaaccga tgatgacggg gtctgtgaac
     1861 atgcgcagaa aataaacatg cagaccaggg taacgcatcc ggtaactagt attctcttca
     1921 taggtaggtt attatatatt gttatatgat cattgtgaca ctttatccct tggcagccat
     1981 accatcatag atcctgcccc cctgtgagcc cacgcatagt agggaatgag ggtcagcaaa
     2041 acctctttgg caaccggaat atagtcgtca ccagcggcct gtaccctggc gggtgtttcc
     2101 agctgtacta taccatacag tttatccggg acgggtactg ccttgaactc cggtgcaggc
     2161 ggcaggagca agctttgcag gtcaaagtca ttatcgggcc attcggcaca gtaaaccacc
     2221 ggaccccttt caacagccag acggcctctg tttgcttcca cttcttcttt ggctgtaacg
     2281 gtccgggctt ccatctggaa agccacttct atggaatcgt cctttttcca tttcctttcc
     2341 atacaaaaat atccgtcaca aagtgttccg tctgctgtct tcccgttcac tttgatgcta
     2401 taagcgggtg ttttgccgtc ggcatatgaa tacagatctc cgggaaccgg ctggttctgc
     2461 acccagcccg gaatacgtac cttgatgatc acgttgtttc ccggtgcttt gtctatggtg
     2521 atagtgatat cgccatccca gggataatcc gtctgttggc gcagaactat atcattatcc
     2581 aaaattttgg tggttagtgt gttggatatg aagagattta cataaaggct gttatccttc
     2641 acggcataaa tatatccggg gagagatggt atgaaccgcg caatattgga gggacagcag
     2701 gcacagccaa accagggttg gcgttggtgc tgacctgcgg attccagtgg attgggatag
     2761 aagaaactgc caccgtccag ggaaaccccc gaaataagcc cgttgtatag cgtacgttcc
     2821 aaaacgtcat aataccccgc cttgccgtgc agcaggaaaa ggcggtgatt cacgtacacg
     2881 ttccctatag ctgcacaggt ttcacaatag gcggtcatat tgggcaactc ataattctca
     2941 ccgaaggctt caccctggtg ggttgcccca atccctccgg tgatgtataa tttctttgtt
     3001 acaatgttct cccagatacg gtctatggca gcgatatagg atttgtcccc ggtaagggcc
     3061 gctacatctg ccatgccgga gtacatgtaa gtggcacgta cggagtgacc gaccgcttcg
     3121 ccctgctgaa tgacgggtat atgtgcctgg ctgtataggt ctttgcggga agtatatccg
     3181 cgtttgtcaa gaaagaattt ggcttgtttc aggtatttct catctcccgt aaccaggtag
     3241 agttttgcca gggccatttc ggctatctgg tggcctggta ctgcaactac ctgtccggga
     3301 ccctccccta tctcactgca gacacaatct gcatagcgta tggcaatatc caggaatgtc
     3361 ctttttccgg tagcctggaa atgagcaatg gcaccttcca ccatgtgtcc caggttgtaa
     3421 aactcatggc tcaattcctc cactttttcc catcttttac ttccggccca ttcgtggggc
     3481 tgttggggat tcatggtgcg ggccgtatac aggtacccgt cgggctcctg ggcagcagcc
     3541 acaagtgcca gaacgctgtc aatataaatt tccagctttt catcaggata agtctgcagc
     3601 aggtaactgg ctccttcaat ggttttgtat acatctgtat catcaaagga aaagcctgta
     3661 acctcatttt ccaagccggg atgggcagcc ttgacgaagt tatcatagcg gccggtttct
     3721 tcgcatttac tgaaagcaag aggaatagtg acctcgcggc tggctttcag gcgttgaccc
     3781 cagaaagcat cggttacttt gacagatgta aaaggcaccg gatcaatggg atagacatgt
     3841 agcacaagcg aaagaaaagg aataatccat agttttatca taattattgc tattttagga
     3901 attcaaatat gatgaaaata tcttttttct ccccgggtat ggtctaattt tatatgaaaa
     3961 tgaattcttt tacacattaa caacctgccc atttttcttt actttgtata aatcaaataa
     4021 tagttacgtt atggcatttg aaaattttga agtatggttt gtaacaggag cacagcttct
     4081 ttacggagga gatgcggtaa ttgccgtgga tgctcattcc acccaaatgg tacagggatt
     4141 gaacgattcg ggtaatctgc ccgtaaaagt cgtttataaa ggaacggtga attcagagaa
     4201 agaggtaacc gccactttca aggaagccaa taattgtgaa aaatgcatcg gggtgataac
     4261 ctggatgcac actttctccc ctgccaaaat gtggatccat ggattgcagg cgcttgacaa
     4321 gccgttgctg catttccata cgcagttcaa taaagagatt ccctgggaca ccatggatat
     4381 ggatttcatg aacctcaacc aaagtgcgca cggagaccgg gaattcggac atatggtgac
     4441 ccggatgcgc aaaaaccgga aagtagtcgc cggtcactgg caggatgaaa aagcacaggc
     4501 tcagattgcc gtctggatgc gggttgccac cgcctgggcc gatgctcatg atatgaggat
     4561 catccgcttt ggtgaccaga tgaacaatgt ggccgtgaca gacggagaca aggtggaagc
     4621 tgagatgcgc ctgggatatc atgtagatta ttgcccgatt gccgaactgg tggccgtgca
     4681 gtcacaggtt accgatgcag aagtggatgc cctggtggct gaatatgaaa tgcaatacaa
     4741 ggtggccgaa aattgccagg ctgggggaaa agaccgtgcc caggttctga gggccgcccg
     4801 cgaagagatt gctctgcgca ggttcctgca ggaaaaggga gccaaggctt ttaccacaaa
     4861 ttttgacgat ctggccgggc tggaccagct acccggactt gcctgccagc gtctcatggc
     4921 tgaaggctat ggttttggcg ctgaaggtga ctggaagacg gccgccctga caaggacggt
     4981 gtggttcatg agccaggaca tgcctaaggg gtgttccttc atggaagact ataccattca
     5041 ttttgacggt aaaaagagtg ctatcctcca ggctcacatg ctggaagtat ctcctttgct
     5101 ggcagacaaa aagccccggc tcgaagttca tccactgggc atcggaggca agaatgaccc
     5161 ggcccgcctg gtgttcacct caaaggaagg cgaaggcatt gctgctaccg tcattgacat
     5221 gggtaaccgt ttccgcctga tagtgaacaa ggtggattgc atcagatcca aatcgctacc
     5281 caaactcccc gttgcttcta ccctgtggat cccccgtccc gattttgaag tggga
//
LOCUS       DAOSOF010000128         5231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_15918_length_5231_cov_5.978555, whole genome shotgun sequence.
ACCESSION   DAOSOF010000128 DAOSOF010000000
VERSION     DAOSOF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15918_length_5231_cov_5.978555"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1333)
                     /locus_tag="PLM68_07230"
     CDS             complement(377..1333)
                     /locus_tag="PLM68_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT55187.1"
                     /translation="MIRFATIGSNVIVDSFLRGAAHDPRFFLQAVYSRTQERASEFAA
                     MHGAGKTFTSLEALAADNQVDAVYIASPNCCHAPQAILMMEHGKHVLCEKPLAPSLAE
                     GKAMAEAARRNGVVLMEAMKTTLMPNFARVREALPHIGTVRRFFAQFCQYSSRYDNFK
                     KGEVSNAFRAGMGTGALRDLGVYGLAPLVHLFGMPQQLQASATLLSTGVDGQGSVLMT
                     YPGMEAVVVFSKISDSFLPSEIQGENGRILIGKLSTMKDPVLVMRDGTTTDLSVPTIA
                     DDMFYEVKEFLDVIASGAKESAVNTHQRSLDVLELVDRAGKL"
     gene            complement(1343..1417)
                     /locus_tag="PLM68_07235"
     tRNA            complement(1343..1417)
                     /locus_tag="PLM68_07235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1380..1382),aa:Val,seq:gac)
     gene            complement(1467..2969)
                     /locus_tag="PLM68_07240"
     CDS             complement(1467..2969)
                     /locus_tag="PLM68_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55188.1"
                     /translation="MSFLPKYSLACMALMAIVACHYAGGEVVPEDSPPAIWPDYKEVT
                     VPVNIAPLNFTLEKTTLLSDEEPAFSRLEAWVEGADGSFIKGSGKRHVRFSMGGWARL
                     LEKNAGDSLLVTLRGWDGKQWHSYRPFTIYVNARPIDHGMVFRYIAPGYEVYSKVDIY
                     QRELSSFRTSAIYENTLIPQTCANCHSFKQGDPEYFSLHVRGEKGATVLQKEGTFRYL
                     DSRTDSTSSAFSYPSWHPDGRYIAYSLNKTYQSFHVTAEDRVEVYDLVSDIVIYDTQE
                     NCILASDLLTTGAFETFPKFSSDGRSLYFCLAREQNLPTEYRDLRYDLCRVDFDPLTG
                     ETGQEIHTVIDAVSIGKSISQPVPSPDSLYILFSLLDFGTFPVNHRASELALLDLEDN
                     TWRLLDAWNSPDVESYHSWSSNSRWVVFSSRRQDGLLVLPYIGYIDEQGNAGKPFLLP
                     QKDPEHFYGTLTRSFNCPELVTAPVKLELRKLERRANRMEREAVQFRRID"
     gene            complement(2947..4971)
                     /locus_tag="PLM68_07245"
     CDS             complement(2947..4971)
                     /locus_tag="PLM68_07245"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT55189.1"
                     /translation="MRKVCSLVFGLGVFLFFWLVLPYHLRFHEQFQFFRFGMDYFMDS
                     VLHPEGFSFYLGGFFTQFFRYPWAGALIIALLLMLMQRLVAAIAYRAQGIASGRWESG
                     SISGSNTGNAPGRVPGNPLWYPLTYLPSAFVWLLLCNENYLISAVVALLLALWAVNLY
                     QRAGDGKSRIAVFYVLQLVLFFLAGGTVLIFTACFLYLEAFCTGEAVPGRTGRMETAC
                     RGTGRWKTYALQVLPAVALVFLYPMAARIFWQYPYEKLLGGLHFYRYYYFSYPLQYVA
                     WALAAAVPLLCRLIPAPKSCSMKRRIPAACPDSVKQQIAAPKPGRGSRIISAVLCVLI
                     TAGTVFGLFRFAGLDKERLFEYDILVYEEQWDQVLQRAQKDTPGSSIEMVAVNLALWH
                     TGRLETELFHYPQQGPEGLMLPFRRDFVTPLMMSQVYLHLGMVNSAQRNAYDAMEAIP
                     DFQKSARSYKLLAQTNIINGHYRVARGYLEQLSKTLFYRKWAQEAMQYLGNEEHFGNE
                     ERLGNEEHLGNEDRLGDEERIAAHPVYGRIRRIRYRDEMLFSPQGLNAMLDILYRNNR
                     ENKMAYAYMLAYTLLAKDLERFADYITLEYPSGSDKTLPVPYQEALALYWVQNNSSLE
                     GIPWNISGDVRKRLDDFTAMYQQFAGGRPERELKKDFGKTYWYYVFFTKI"
BASE COUNT         1283 a         1390 c         1372 g         1186 t
ORIGIN      
        1 aacgttaaca ggtgaatatt taccgactga gtaaatatat taaaaaattg aatttaatcc
       61 aaataaccgg atgagacact atacatgtca tccacaattg tgggcactaa aagctctgtt
      121 gtggtcccat cccgcactac cagcattcct gctcaaacaa gcgcccggtg ctattcaagg
      181 cggtgcgcca aatgctcttg gcgtttttgg cgtttataga ggtgctgctt cgcagcatca
      241 atcatttttt gcgagtatac taaaagcagg gataactgcc aaaagtcccc aattgctttc
      301 tgaattcatg gattgaccaa aagaggttcc acttgtgtcc ggttccacct gtttgcgagt
      361 tccaccctcc ccggtgttac agtttcccgg ccctgtccac cagctccagt acgtccaggc
      421 ttctttggtg cgtgttgacg gcagattctt tggcgccgga tgcaataacg tccaggaatt
      481 ccttcacttc ataaaacatg tcgtccgcaa tggtcggcac cgaaaggtct gttgtggttc
      541 cgtcccgcat gaccagcacg gggtctttca tggtggaaag cttgccaatg aggatccttc
      601 cgttttcccc ctggatctcg gatggcagga aagaatccga gatcttggag aacaccacca
      661 ccgcctccat cccgggatag gtcatcaaca ccgagccttg tccgtccacc ccggtggaga
      721 ggagtgttgc cgaggcctgc agctgttgcg gcatgccaaa gagatgcacc agcggcgcca
      781 gcccgtaaac acccaggtcc cgcagcgctc ctgtccccat tccggcccga aatgcattgc
      841 ttacctcgcc tttcttgaag ttgtcgtaac gcgaggaata ttggcagaac tgtgcaaaaa
      901 accttcttac cgtaccgatg tgcggcaagg cttcccgcac gcgggcaaaa ttgggcatca
      961 gggtggtctt catggcctcc atgagcacta ccccgttgcg gcgggccgcc tctgccatgg
     1021 ccttaccttc tgccagcgac ggtgccagcg gtttttcaca aagcacgtgt ttcccgtgct
     1081 ccatcatcag gatagcctgc ggggcatggc aacaattggg agaggcaatg tagacggcat
     1141 ccacctgatt gtcggcagcc agtgcttcca gcgatgtaaa ggttttcccg gctccgtgca
     1201 tggcggcaaa ttcgctggca cgctcctgtg tccgggaata aacagcttgt aagaaaaaac
     1261 ggggatcatg agcggctccc ctcagaaagg aatccacgat cacattggaa ccaatggtgg
     1321 caaaacggat cataggaaaa agtgagttgt actggattcg aaccagtgac ccctaccctg
     1381 tcaaggtagt gctctaaacc agctgagcta acaactcaat gtgtcaaggc gcgcaaatgc
     1441 tgggcaaaaa tacaaaaaag aaccaattaa tcaatacgcc ggaattgcac cgcttcgcgc
     1501 tccatccggt tggcccggcg ttccagtttg cgcagttcca gcttcaccgg agcggtgacc
     1561 aactcgggac aattgaaaga acgggtaagg gtcccgtaaa agtgttccgg gtccttctgg
     1621 ggcaagagga acggtttgcc cgcattgccc tgttcatcga tatatccgat gtaaggcagc
     1681 accagcaatc cgtcctgccg ccggctggaa aaaaccaccc acctggaatt gctgctccag
     1741 ctgtgatagc tttccacatc ggggctgttc caggcgtcaa gcaaacgcca tgtgttgtct
     1801 tccaggtcca atagggccag ttccgaggcc ctgtgattca ccgggaacgt gccgaaatcc
     1861 agcagggaaa aaaggatgta caggctgtcg ggtgagggaa cgggctggga aatgcttttt
     1921 ccaatggaaa cagcgtcgat gaccgtatgg atctcttgcc cggtttcgcc ggtgagcgga
     1981 tcaaaatcca cccggcacag atcgtacctc aggtcccggt attctgtggg caggttctgt
     2041 tccctggcca ggcagaaata cagggaacgc ccgtcggaag aaaattttgg aaacgtctca
     2101 aaagccccgg tggtcagcag gtctgatgcc agtatgcaat tttcctgcgt atcgtagatc
     2161 acaatgtccg agaccaggtc atacacttcc acccggtctt ctgctgtcac gtggaaggat
     2221 tggtaggttt tgttgagcga ataggcaatg tagcgtccgt cgggatgcca ggaaggatag
     2281 gaaaaggccg aggaggtgga gtcggtccgg ctgtccaggt atcggaatgt tccttctttc
     2341 tgcagcactg tggctccctt ctctcccctt acgtgaaggg aaaaatattc gggatcaccc
     2401 tgtttgaagg aatggcaatt cgcgcaggtt tgagggatca gggtgttttc ataaatggcg
     2461 ctggtgcgga acgaggacag ttcgcgctgg tagatgtcca ctttactgta cacttcgtac
     2521 ccgggggcaa tataacgaaa aaccattccg tgatctatgg gccgggcgtt tacgtaaatg
     2581 gtgaaggggc ggtaactgtg ccattgcttt ccatcccagc ccctgagtgt aaccagaaga
     2641 ctgtctcccg cattcttttc cagcaaacgg gcccaaccgc ccatggaaaa acggacgtgc
     2701 cgctttccgg accctttgat aaaacttcca tcggccccct ccacccatgc ttccaggcgg
     2761 ctgaatgcag gctcctcatc gctaagcaac gtggtctttt ccagggtaaa attcagggga
     2821 gcgatattga ccggtaccgt tacttccttg taatcgggcc atatggccgg aggggaatcc
     2881 tccggaacca cttctccgcc tgcataatgg cacgcaacga tggccatcag ggccatgcat
     2941 gccaggctat attttggtaa aaaagacata ataccaatac gtttttccaa aatccttttt
     3001 cagttccctt tcaggccttc cgcctgcaaa ctgttgatac atggctgtaa aatcatccag
     3061 tcttttgcgt acgtctccgg atatgttcca ggggatgcct tccagcgagc tgttgttctg
     3121 tacccagtac aaagccaggg cttcctggta tggaacggga agggttttat cacttccgga
     3181 gggatattcc agggtaatgt aatcggcaaa gcgttccagg tccttggcca gtaaggtata
     3241 ggccagcatg taggcgtaag ccatcttgtt ctcccggttg ttccggtaca ggatatccag
     3301 catggcattg agtccctggg ggctgaagag catttcgtcc cggtaacgga tcctgcggat
     3361 gcggccatag acaggatgtg ctgctatacg ttcttcatct ccaagtcggt cctcatttcc
     3421 aaggtgttcc tcatttccaa ggcgctcctc atttccaaag tgttcctcat ttccaaggta
     3481 ttgcatggcc tcttgcgccc atttccggta aaagagggtc tttgaaagct gctccaggta
     3541 accgcgggct acccggtaat gaccgttgat tatgtttgtt tgggcaagga gtttataaga
     3601 ccgggcactc ttttgaaaat cggggatcgc ctccatggcg tcgtaggcgt tccgttgtgc
     3661 agaattgacc atgcccaggt gaaggtacac ctgggacatc atcaaagggg tcacgaaatc
     3721 ccggcgaaaa ggaagcataa ggccttcggg accctgttgc gggtagtgga acagctccgt
     3781 ttccaggcgg ccggtatgcc ataaggccag gttgacggcc accatttcaa tggaggaacc
     3841 gggggtatct ttttgcgctc gctgcaggac ctggtcccat tgctcttcat acacaagaat
     3901 gtcgtattca aagagccttt ccttatccaa cccggcaaaa cggaacaggc caaacacggt
     3961 cccggcagtg atcagcacac acaacaccgc agaaatgatc cggcttccac ggccaggttt
     4021 tggcgcggcg atctgctgtt tcacgctatc agggcacgcg gcggggatcc ggcgtttcat
     4081 gctacaagat tttggagcgg ggatcagccg gcacaacagg gggacggcgg ctgccagtgc
     4141 ccaggccacg tactgcaggg ggtatgaaaa atagtagtat ctatagaaat ggagccctcc
     4201 cagtagtttt tcatagggat actgccagaa aatacgcgcg gccataggat acaggaaaac
     4261 aagcgccact gccggaagca cctgtagggc atacgttttc cagcggcctg tgcctcggca
     4321 agcggtctcc atgcgaccgg tccttccggg aactgcttcc ccggtgcaaa acgcctccag
     4381 gtagaggaaa caggccgtaa agatgagtac cgttccgccg gccagaaaga acaataccaa
     4441 ttgcaggaca taaaatacgg cgatccttga ttttccgtcg ccagcgcgct ggtacagatt
     4501 cactgcccac agggccagca acagggccac caccgccgag atcaggtaat tttcattgca
     4561 caaaagcagc cagacaaaag cagagggcaa atatgtgagt ggataccaga gagggttgcc
     4621 gggtactctg ccaggtgcat tgccggtatt actgccggat atgctgccag attcccatct
     4681 gccggatgca ataccctgcg ccctgtatgc aatcgctgcc accaaccgtt gcataagcat
     4741 cagcaacaag gctataatga gagctccggc ccacgggtac cggaagaatt gggtaaaaaa
     4801 accgcccagg taaaaggaaa agccttccgg atgcaggacc gagtccatga aataatccat
     4861 cccaaaccgg aaaaactgga actgttcgtg aaaccgcaaa tggtacggaa gcactaacca
     4921 gaaaaaaaga aaaacaccca gcccgaatac cagtgaacag acttttcgca taagacaaag
     4981 ttaagaaaaa gcttcgcttg cggtgcgcca aacgtgtttg gcgcgtataa aatgctgctt
     5041 cgcagcacca atttgttttt ttatgcccct gtgggtcaga attgcacata atccgccggt
     5101 ttgtccacag aaacagcctt atatgtggat gaaaggcaga aaatcccggg ttcctccaca
     5161 aaaacagtcc tttatgtgga taagatgcca taaaacgaag gttcctccac aaaaatggcc
     5221 ttatatgtgg a
//
LOCUS       DAOSOF010000129         5210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16012_length_5210_cov_4.416683, whole genome shotgun sequence.
ACCESSION   DAOSOF010000129 DAOSOF010000000
VERSION     DAOSOF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16012_length_5210_cov_4.416683"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLM68_07250"
     CDS             complement(<1..581)
                     /locus_tag="PLM68_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55190.1"
                     /translation="MNRYTSLLLLFLSMIPLSLRAQTEVMAWGNITGIRVDGHLMAFE
                     SFLTVGDKNMDKTSFSGKEKQPHPLYHREGLKQIVTTDLRGVNFKQEVTDTGPGTVQV
                     RITSTCDTATPSHNAWLAIKLDNCSYHISGRNLVLKNQEVTLTLSFTRRPTLVSKGDL
                     IFILLLSELKKGRSATLEFNLCARGLIDKDPVT"
     gene            complement(586..2301)
                     /locus_tag="PLM68_07255"
     CDS             complement(586..2301)
                     /locus_tag="PLM68_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55191.1"
                     /translation="MKKIILSLLCLLFFCHAGAQQYKNFKVSVYTRAYEVEKMKDSHW
                     LDSTWRIIAAQVQPDKIYLETHRDLLIVPDATLRKAIRFFREKGLEVGGGITYTIDES
                     NSFETFCYTNPEHRKKVQEIAEHTARYFDDFILDDFFFTSCKCPLCIEAKGSMSWTEY
                     RLRLMTEAGKSLVLDPARKVNPNVRVIIKYPNWYDHFQGLGFDLEHGPSLFDGVWTGT
                     ETRDPSFAQHLQNYLSYNVFRYLDNLRPGYNLGGWVDSGGSHMGMYRYAEQLWLTLFA
                     KAPEITLFAYNQLIRQPELGKTATETFLLTDQFLGELGKPQGIASYKPFHATGEDFLQ
                     NYLGMIGLPMDMLPYFPTDKKVVLLTEQAKSDPAIADKIRQQLMRGGDVVVTTGLLKA
                     IPDQIASVAEVRCSDLKALIDDFGWNGKASKDMLIPQVHYLTNDAWEIVSAGKPLTQG
                     VIGYPILLRCPFAKGNMYILTIPDNVADLYAFPDNALNMIRFFMSRDLDVRIEGPSMV
                     GLFVYDNGTFIVESFRDEPVSVHVVLSGEQEKVTELTENKAYSVESDKFSITLEPHTF
                     KVFKK"
     gene            complement(2329..3795)
                     /locus_tag="PLM68_07260"
     CDS             complement(2329..3795)
                     /locus_tag="PLM68_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55192.1"
                     /translation="MRKILIIPALAAILWSGCTADISKAELRTAPPQIFPDYCEVTVP
                     CNIAPMHFQMSDNAIQMLVTAEGADGCVIQVKTKKVVTFGQSAWKDLVASSAGSGIKV
                     TVHAKYREEGWVRYEPFTIHVSPVPINDYLVYRLIAPGYEVYSKMGIYQRDLTSFRET
                     PLLENTLIPGNCMNCHSFSGNHPEKMSIHVRGELGGTILKTGDKIEMLNTKTDETGGN
                     CVYPYWHPSGEYVVYSVNQTIQAFHSIRERRIEVIDLSSDIVVYHPQSKQILTPDLLR
                     TDAFETFPSFSPDGRTLYFSSSQFQNIPEAYEKIRYDLCSIAFDPQTGAFGDHVDTLI
                     HAAAMGKSISFARPSPDGKYIMFTLSDYGNFSIWHKEADLWLYDLKDGSLREMKEVNS
                     DDVESYHSWSSEGTWFVFSSRRLDGLYTRPFFASIDKEGNITKPFLLPQCKPLEFYNM
                     NLYSYNVPEFITEKVDWDLKKVEKALNTGQRDKVEVRR"
     gene            complement(3792..>5210)
                     /locus_tag="PLM68_07265"
     CDS             complement(3792..>5210)
                     /locus_tag="PLM68_07265"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOT55193.1"
                     /translation="LLCNENFLTGGIVSILVALGAWLAYFYLKNTTTRRLYVMLAIPV
                     IYWLAGGSAFIFTFGCLAVEWLRDESSRGRIMTTGLSILIMGLCLFTAKVILPQYPFA
                     RLIYGADFFRFPKTYPSGVWVMWLFCLVVPLSHLMLPAVFKKTRNLALAGIVTLIAVA
                     GPGAYMISINTDTAKEDIMAYDHYVRMQQWDKAIARADRKAPSSPLSVACLNLSLCKS
                     GMMSDYMFRYYQNGPEGLIPTFARDFTLPMVTGEVYYHLGFINTAQRFAFEAMESLPN
                     YWKSGRALKRLAETNLINGQYAVARKYLEILQHTLFYKAWANHVIPYLEDEALIGGHP
                     EWGNLRKHRTKTDFFDSEQEKDMMLGILLQQDLTHYMAYEYLLAYCLLTKDLERFVQY
                     FPLGSELRYERLPYSYQEALLYHWSLSHEDPFRTVPYPISEDVMRNMATYQNLYLNSS
                     NPEEVLKKNHSGTYWYYLHFRK"
BASE COUNT         1305 a         1214 c         1296 g         1395 t
ORIGIN      
        1 attgttaccg ggtccttgtc tatgagaccc ctggcacaaa ggttgaattc cagggtggct
       61 gatcttcctt tttttagctc tgaaagcaag agtatgaaga taagatcccc tttgcttacc
      121 aacgttggtc tacgggtaaa ggaaagagtc agggttactt cctgattttt caggaccagg
      181 tttcttcctg aaatgtggta actgcaatta tccagtttaa tagccaacca tgcattatgg
      241 gaaggagtgg ccgtgtcaca ggtggacgtt atcctgactt gaactgtgcc cggtcccgta
      301 tcggttactt cctgtttaaa attcaccccg cgtagatcgg tggtaacaat ctgcttaaga
      361 ccttccctgt ggtataacgg atgtggctgt ttttcttttc cggaaaaaga agtcttatcc
      421 atgttcttat ctccgacagt caggaatgat tcgaatgcca tcaggtgccc gtcaacgcgg
      481 atgccggtaa tgtttcccca agccatcact tctgtctggg ccctgagcga aagggggatc
      541 atacttaaga ataatagaag cagggaagta tatctgttca tgtgcttatt ttttaaaaac
      601 cttaaaagta tggggttcca gggtgattga aaatttgtcg gattcaacgg aatacgcttt
      661 attctctgtc aattccgtta ctttttcctg ttccccggaa agaaccacat ggacagatac
      721 cggttcgtcc cggaaagatt ccacaatgaa agttccgtta tcgtagacga ataaacccac
      781 catggaaggg ccttcaatgc gtacatccag gtccctgctc atgaaaaacc ggatcatgtt
      841 cagggcgttg tccggaaatg cataaaggtc ggcaacattg tccgggatgg tgagtatgta
      901 catgttccct ttggcaaaag ggcacctgag caggatggga taacctatga caccttgtgt
      961 caggggcttg ccggcagaaa caatctccca tgcatcattg gtcaggtaat gtacctgtgg
     1021 aatgagcatg tctttggaag ccttcccatt ccaaccaaaa tcgtctataa gtgctttcag
     1081 gtctgagcac cttacttctg caacagaggc gatctggtcc gggatggctt tcaaaaggcc
     1141 agtggtaacg actacatcgc ccccacgcat cagttgctgc cggatcttgt cggctatggc
     1201 gggatcagat tttgcctgtt cggtcagcag gaccaccttc ttgtcggtag ggaaataggg
     1261 gagcatgtcc ataggcaggc ctatcatacc caggtaattc tgcaggaaat cttctccggt
     1321 tgcatggaaa ggtttatagg aagcaatacc ctgcggtttt cccagttctc ccaaaaactg
     1381 atctgtcagc aggaaggttt ctgtagctgt cttacccagt tctggctgcc ggatcagctg
     1441 gttataggca aaaagggtga tttcaggcgc tttggcaaac agagtcagcc atagctgttc
     1501 tgcgtaacgg tacattccca tatgggaacc gccactatcc acccagccgc ccaagttgta
     1561 tcccggtcgc aggttgtcaa ggtacctgaa aacgttataa cttaaatagt tttgcaggtg
     1621 ctgcgcaaag gaagggtccc tggtctctgt tcctgtccac accccgtcaa ataaagaagg
     1681 gccgtgttcc aggtcaaatc caagaccctg gaaatgatcg taccagttgg gatactttat
     1741 gataacgcgt acgttgggat tcaccttgcg ggccgggtcc agaaccaggc tttttccggc
     1801 ttctgtcatg agccggaggc ggtattctgt ccagctcatg ctgcctttgg cctctatgca
     1861 aagaggacat ttgcagctgg taaaaaagaa atcgtccagg ataaaatcat caaagtaacg
     1921 ggctgtgtgt tcagcaattt cctgtacttt tttacggtgt tccgggttgg tataacaaaa
     1981 agtctcgaaa ctgttggatt catctatggt ataggttata ccgccgccga cttccagtcc
     2041 tttttccctg aaaaagcgaa tggctttacg cagggtggca tccggaacaa tcagcaggtc
     2101 ccggtgggtc tccaggtaga tcttgtcggg ctgtacctgg gcagcaatga tcctccaggt
     2161 ggaatccagc cagtggctgt ctttcatttt ctcgacttcg taggcacgtg tatagaccga
     2221 aaccttaaaa ttcttgtatt gttgcgcccc ggcatgacaa aaaaacaaca ggcacaacaa
     2281 tgacagtatg attttcttca tagcaaaagt gtattgattt gttatttatt acctgcggac
     2341 ctccaccttg tccctttggc ctgtattcag ggctttttca acttttttca gatcccagtc
     2401 caccttttcg gtaatgaatt cggggacatt gtatgagtac aggttcatat tgtagaattc
     2461 caggggtttg cactgtggca gcaggaaagg tttggtaata tttccctctt tgtcaatgct
     2521 tgcaaagaaa ggtcttgtat agagcccgtc aagacgccgg ctgctgaaga caaaccaggt
     2581 gccttccgag gaccagctgt ggtaactttc cacatcgtcg ctgttcacct ctttcatttc
     2641 ccgtaacgat ccgtctttca ggtcatagag ccataaatca gcttctttgt gccaaatgga
     2701 gaagttgcca taatcagaca gcgtaaacat gatgtatttg ccatccggag atggcctggc
     2761 gaaggaaatg cttttgccca tagcagcggc atgtatcagg gtatcgacat gatcaccaaa
     2821 cgctccggtt tggggatcga aggcgatgga acacaggtca tagcggatct tctcgtaagc
     2881 ctcgggaatg ttctgaaatt gtgaggagct gaaatacaag gttctgccgt caggagaaaa
     2941 ggatgggaaa gtttcaaagg catctgtccg gagcagatca ggagtcagga tttgtttgct
     3001 ttgcgggtgg taaactacaa tatctgagga caaatcgatg acttctatac gacgttccct
     3061 gatggaatga aatgcctgga tggtctggtt gaccgaataa acgacatact ctccagaagg
     3121 atgccagtat ggatatacgc aattacctcc ggtttcatct gtcttggtat tcaacatttc
     3181 tatcttgtca cctgttttga gaattgttcc tcccagttcg ccacggacat ggatgctcat
     3241 cttctcagga tgatttccgg aaaaagaatg acaattcatg caattgcccg gaataagcgt
     3301 gttttccagc aggggtgtct cccggaaaga agtaaggtcg cgctggtaga tgcccatctt
     3361 gctgtacacc tcatacccgg gcgcgatcag gcggtatacc aggtaatcgt ttatgggtac
     3421 tggactgaca tgaatggtaa atggctcgta gcggacccag ccctcttccc tgtattttgc
     3481 atggactgtg accttgatac ctgacccggc actggaggcg accagatcct tccaggctga
     3541 ttgcccaaaa gtcactactt ttttggtctt tacctgtata acacagccgt ctgcaccttc
     3601 tgccgtcacc agcatttgga tggcattgtc gctcatctgg aaatgcatgg gggcaatatt
     3661 gcagggaacg gtcacctcgc aataatcagg gaaaatttga gggggggctg tcctgagctc
     3721 cgccttggaa atatctgcgg tacatccgct ccacaggata gcggctaatg ctggtatgat
     3781 cagtatcttt ctcattttct aaagtgtaag taataccaat aggtgcccga atgattcttt
     3841 ttaagtactt cttccggatt ggatgagttc aaatacaggt tctggtaggt tgccatattg
     3901 cgcatgacat cttcacttat aggataaggc acggtacgga atggatcctc atggctcaag
     3961 gaccagtggt agagcaaggc ttcctggtaa ctgtatggca ggcgttcata acgcagttcg
     4021 gagcccagtg ggaaatactg tacgaaacgt tccagatctt ttgtcaggag gcaataagcg
     4081 agcaggtatt cgtatgccat gtaatgcgta aggtcttgct gaagcaggat tcccagcatc
     4141 atgtcttttt cctgttccga atcaaagaaa tcggtcttgg tacgatgttt tctaagattc
     4201 ccccattccg gatgcccgcc tatgagcgct tcatcttcca ggtatggtat cacatgattg
     4261 gcccaggcct tgtagaacaa cgtatgctgg aggatttcca ggtatttgcg ggctaccgcg
     4321 tactgaccgt tgatcaggtt ggtttcagcc agccttttga gggcgcgccc gcttttccag
     4381 taattgggca atgattccat ggcttcaaag gcaaaccttt gggctgtatt gatgaatccc
     4441 aagtggtaat agacctcgcc tgtaaccata ggtaaggtga aatccctggc aaaggtgggt
     4501 atcagccctt ccggaccgtt ctggtaatac cggaacatgt aatcggacat catgccgctc
     4561 ttgcacaggg aaaggtttag acaggcaact gacagcggtg aagaaggggc tttccggtct
     4621 gccctggcta tcgccttgtc ccattgctgc atcctcacat aatggtcgta ggccataata
     4681 tcttctttag ccgtgtcggt gttgatggat atcatataag cacccgggcc agccacggca
     4741 atcaatgtga cgatccctgc caaagccagg tttcttgttt ttttgaaaac ggccggcaac
     4801 atgagatgcg acaggggaac gaccaggcag aaaagccaca tgacccagac gccggaagga
     4861 taggtttttg gaaagcggaa aaagtctgct ccataaatca ggcgggcaaa cggatattgg
     4921 ggcaggatta ctttggccgt aaacaggcat aatcccatga taaggatgga gagccctgtt
     4981 gtcataatac gacctctgga ggattcatcc cttaaccatt ccacagccag gcatccaaat
     5041 gtgaaaataa aggccgaacc ccctgctaac caatatatta cgggaatggc taacatgacg
     5101 tacagcctcc gtgtggtggt atttttgaga taaaaatatg ccagccatgc acccagcgcc
     5161 acaagaatgg aaacgatacc ccctgtaaga aaattctcat tgcacagcag
//
LOCUS       DAOSOF010000130         5180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16157_length_5180_cov_4.513951, whole genome shotgun sequence.
ACCESSION   DAOSOF010000130 DAOSOF010000000
VERSION     DAOSOF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16157_length_5180_cov_4.513951"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLM68_07270"
     CDS             complement(<1..1513)
                     /locus_tag="PLM68_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOT55194.1"
                     /translation="MTELEHKRLIAKNTAALYVRMLVRLAITLYTSRIVLDILGVDDF
                     ALYGIVGGIVTLLTFPQTALNASTQRFMSIELGRGDTAALKRVFDGSMTLYLLVALVI
                     VILAETAGLWFLNTRLVIPPDRLNAVNWVFQFSILVTCVNIIQTPYNASIIAHEKMSF
                     YAWISIADVVLKLGVVFVLMLSPVDKLVSYAALLFAVSLVIFFITRGYALRQFNVCRF
                     KPSWDKQLLKKMAAFSGWTLCDSGAQLAVTQGRVFLLNIFTPLVTNAAMSIATQVSTA
                     IYNFVYYFQSSFNPQITKASAQGNQGYLQDLVNQTSKFSYYLIFALTLPILLNMEFIL
                     GIWLKGVPQYTAVFCSITLLYYLVESLSGPLWITTWAAGNIRRYQVTISLLRITTLPA
                     TWLLLKYGMEPWTVLAVWVLVNGFCYIYRLMYVRRNSGIPLNPYVREVIIPTFLITLL
                     AVPLPLLAHYRLAGWTDLVITTLVSLLITGMLIWLIGLKPTEKKHIKEFVKKRL"
     gene            complement(1531..2850)
                     /locus_tag="PLM68_07275"
     CDS             complement(1531..2850)
                     /locus_tag="PLM68_07275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOT55195.1"
                     /translation="MNIVIVGTGYVGLVSGTCFAEMGAHVTCVDIDRQKIEHLKKEIL
                     PIYEPGLESMVQRNVQAGRLHFTTDITKCLDQAEIVFSAVGTPPNEDGSADIHYVLDV
                     ARTVGKHMTGYLVFTTKSTVPVGTAAKVKQAIQQELDKRNANIPFDIASNPEFLKEGD
                     AIRDFMSPDRVVVGVESEKARKIMENLYRPFLLKNFRVLFMDIPSAEMTKYAANAMLA
                     TRISFMNQVANLCERVGANVNSVRTGIGSDSRIGNKFLYPGAGYGGSCFPKDVKAMIR
                     MGRETGYPLELIESVDQVNEEQKKMLFHKLQKHFKGELKGKTIAVWGLSFKPQTDDIR
                     QAPSLVLINSLLQAGCHVQAYDPVAIEQARHLLGNSIHFADDMYQAANEADALVLVTE
                     WKEFRMPAWDVLHKIMHTPLVLDGRNIYNPQEVREKGFEYIGIGLGA"
     gene            complement(2847..3923)
                     /gene="rfbB"
                     /locus_tag="PLM68_07280"
     CDS             complement(2847..3923)
                     /gene="rfbB"
                     /locus_tag="PLM68_07280"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT55196.1"
                     /translation="MKDNKNNILKGSGNFTVLITGGCGFIGSHVVRRFVKQYPQYLVI
                     NVDKLTYAANPEYLRDIDREPNYRFIQADVCDHAKMEEIFRQYSIDAVIHLAAESHVD
                     RSITDPFAFARSNVMGTLTLLQTAKTHWKKEDYHRHLFYQVSTDEVYGQLNPGEEPFR
                     ETTPYDPRSPYSASKASADHFVRAFHHTYGLPVRISNCSNNYGPCQYPEKLIPVIIHN
                     IVNRKPLPVYGKGENIRDWLYVEDHARAIDLVFHEGRDGETYNIGGNNEWQNIELVRL
                     LIEITDRLLDRPAGDSLPLISFVTDRPGHDLRYAIDAAKIKTELGWQQEMSFRQGLEK
                     TVAWYLDQENLRDASAPSPKTKIV"
     gene            complement(3951..4943)
                     /gene="rfbA"
                     /locus_tag="PLM68_07285"
     CDS             complement(3951..4943)
                     /gene="rfbA"
                     /locus_tag="PLM68_07285"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT55197.1"
                     /translation="MKGIILAGGSGTRLYPITKGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIISTPQDLPSFERLLGTGEAFGVRFQYAPQPRPEGLAQALIIGRDFTGNDNV
                     CLVLGDNIFYGQGFRAMLQRAVASANNGFAHVFGYPIKDPHRYGVVELGPAGIPVSIQ
                     EKPQKPRSNLAVTGLYFYPNNVLDVAAAVKPSARGELEITTVNEHYLNLRHLHVTTLG
                     RGFAWLDTGTHSSLAEASNFVETIQARQGLKIACLEEIALRNGWRTPEQIHTDALPMQ
                     HNEYGQYILELAAAAQQTGLLAQQDDEAGASGVTLAQDIAVPAQTGSSKTSGEN"
BASE COUNT         1089 a         1274 c         1513 g         1304 t
ORIGIN      
        1 gcaaacgttt ctttacaaat tctttgatat gctttttttc cgtgggtttt agcccgatga
       61 gccagataag cattccggta atcagcaggg aaaccaatgt tgtaattacc aggtctgtcc
      121 atcctgccag gcgataatgg gccagcaggg gcaggggtac ggcaaggagc gttatcagga
      181 atgtgggtat gataacttcg cggacgtagg ggttcaaagg gatccctgaa ttccgcctaa
      241 cgtacatcag tctgtaaata taacaaaacc cgttgacaag cacccagact gccagtaccg
      301 tccaaggttc catgccgtac ttgagcagga gccaggtggc cggcagggtg gttatgcgga
      361 gcagggaaat ggtcacctgg tagcggcgta tattcccggc tgcccaggtg gttatccaca
      421 acgggccgct cagggattcc accaggtagt acaacagggt tatgctgcag aagacggccg
      481 tgtattgggg cactcccttt agccatatcc ccaggatgaa ttccatattg agcaagatgg
      541 gcagggtgag tgcaaagatc aagtaatagg agaatttgga ggtctggttg accaggtcct
      601 gcaaatagcc ctggttgccc tgggccgagg ctttggtgat ctgggggttg aatgaggact
      661 ggaagtagta gacgaaattg tatatggctg tgctgacctg ggtggctatg ctcatagcgg
      721 cattggtcac cagcggtgtg aatatgttga gcaaaaatac acgcccttgt gtgacagcca
      781 gctgggctcc ggaatcgcat agcgtccagc ccgaaaaggc ggccattttt ttcaatagtt
      841 gtttgtccca ggaaggtttg aagcggcaga cgttgaattg gcgcagggcg tagccccggg
      901 tgatgaaaaa aatgacgagt gaaactgcaa acaataaggc ggcgtaagac accagcttgt
      961 cgaccggtga aagcatcagc acgaagacaa cacccagttt caacaccaca tcggcaatgc
     1021 taatccaggc ataaaaggac attttttcat gggcaatgat actggcattg taaggggttt
     1081 ggatgatgtt cacacaggtg accaggatgg agaactggaa gacccagtta accgcgttca
     1141 ggcggtcggg cgggatgacc aggcgggtgt taaggaacca caggccggct gtctcggcaa
     1201 ggataacaat gacaagtgcc accagcaggt aaagcgtcat gctcccgtca aatacgcgct
     1261 tcagggcagc agtgtccccg cgtccgagct caatgctcat gaagcgttgg gtggaagcgt
     1321 tcagggcggt ttgcgggaat gtgagcaggg tgacgatgcc tccgactatg ccgtaaaggg
     1381 caaaatcgtc cacacccagg atgtccagga caatgcgcga ggtgtaaagc gttatggcca
     1441 ggcgcaccag catgcgcacg taaagggcag cggtgttctt ggctatgagt cttttatgct
     1501 caagttctgt catatgctgt atgcgacttc ttaggcaccc agtccgatac ctatgtattc
     1561 aaagcctttc tccctgactt cctgcggatt gtaaatattg cgtccgtcca gcaccagggg
     1621 agtgtgcatg atcttgtgca gtacgtccca tgcgggcatg cggaattcct tccattcggt
     1681 caccagcacc agggcatcgg cctcgttggc tgcctggtac atgtcgtcag caaaatggat
     1741 ggaattaccc agcaggtgcc tggcctgttc tatggccacc gggtcgtaag cctgcacgtg
     1801 gcagcctgct tgcagcaaac tgtttatgag taccagggag ggtgcctgac ggatgtcgtc
     1861 tgtctgcggt ttgaaggaaa gaccccacac tgcaatggtc ttgcccttga gttccccttt
     1921 gaagtgtttt tgcaacttat ggaataacat ttttttctgt tcttcattaa cctggtcgac
     1981 tgattctatg agttccagtg gatagcccgt ttcgcgtccc atccggatca tggctttgac
     2041 gtctttggga aagcagctgc ctccgtagcc tgccccgggg tacaggaatt tattgcctat
     2101 acggctgtcc gaaccgatac ccgttcgtac gctgttcacg ttggcgccca cgcgttcaca
     2161 caggttggcc acctggttca tgaacgagat gcgggtggcc agcatggcat tggcggcgta
     2221 tttggtcatc tcggccgaag gaatgtccat gaagagtacc ctgaaattct tgagcaggaa
     2281 aggccggtag aggttttcca tgatctttct tgccttttca ctttccacgc ccaccaccac
     2341 gcgatccggg ctcatgaaat cgcggatggc atcgccttct ttcaggaatt cggggttgga
     2401 ggcaatgtca aaggggatgt tggcgtttcg tttgtccagt tcctgctgga tggcctgttt
     2461 cactttggcg gccgttccca cagggactgt gcttttggtg gtgaagacca ggtagccggt
     2521 catatgcttt ccaacagtcc gcgctacatc cagcacgtaa tggatatcgg cgcttccgtc
     2581 ttcgttaggg ggcgtaccca cggcgctgaa aacgatttcc gcctggtcca ggcactttgt
     2641 gatatctgtt gtgaaatgca gccttccggc ctggacattc cgttgtacca tggattccag
     2701 tcccggctca tagatgggta ggatttcttt cttcagatgt tcaatttttt gccggtcaat
     2761 gtctacacag gttacatggg ctcccatttc tgcgaaacag gtaccggaaa caagtcctac
     2821 gtacccggtt cctacaataa caatattcat acgatttttg ttttgggtga aggggcagaa
     2881 gcatcccgca gattttcctg gtccagatac catgccacgg tcttttccaa tccctggcga
     2941 aaggacattt cttgttgcca gcccagttcg gttttgatct tggcggcatc tatggcgtag
     3001 cgcaggtcgt gaccgggacg gtcagtcaca aaggagatga ggggcaggga atcgcccgca
     3061 ggacggtcca gcaggcggtc ggtgatttct atgagcaggc gtaccagctc tatgttctgc
     3121 cattcgttgt tgccgcctat gttgtaggtt tctccgtcgc gtccttcatg aaataccagg
     3181 tctatggccc gggcatggtc ttccacgtaa agccagtccc ggatgttttc tcctttgccg
     3241 tagacgggca ggggcttgcg gttcactatg ttatggatga ttacggggat aagtttttcg
     3301 gggtactggc agggtccgta attgttggaa caattggaaa tcctgacagg cagcccatag
     3361 gtgtggtgaa atgcccttac aaagtggtct gccgaggctt ttgatgccga gtagggggag
     3421 cgggggtcgt agggggtggt ttcacgaaac ggttcctctc cggggttcag ttgaccgtag
     3481 acttcgtctg tggatacctg gtaaaacagg tgcctgtggt agtcttcctt tttccagtgt
     3541 gttttggccg tttgcagcag ggtgagcgtg cccatgacgt tggaacgggc aaaggcaaac
     3601 ggatcggtga tgctgcggtc cacgtggctt tcggcggcca ggtggatgac tgcgtctata
     3661 ctgtattgcc ggaagatctc ctccattttg gcatggtcgc agacatcggc ctgaatgaaa
     3721 cggtagttgg gctccctgtc gatatccctg aggtattccg ggttggctgc ataggtgagc
     3781 ttgtccacat tgatgacaag gtactgcggg tattgtttga caaaacgcct gaccacgtgc
     3841 gagccaatga atccgcagcc accggtgatg agaaccgtga agttcccgga acctttcaaa
     3901 atattgtttt tattatcttt catatgtctt gccttattgt tgcagggctc tcagttttct
     3961 ccggacgtct tgctgctccc ggtttgtgca ggcacggcta tatcttgcgc taaagttact
     4021 ccgcttgctc cggcttcatc atcttgctgt gccagcaagc ctgtctgctg tgctgccgcg
     4081 gcgagttcca ggatgtattg gccgtattcg ttgtgttgca tgggaagtgc atcggtgtgg
     4141 atctgttccg gggtgcgcca gccgttgcga agggcgattt cctccaggca ggcgatcttt
     4201 aggccttgtc gtgcctggat ggtttccaca aagttagagg cctcggcaag agaactgtgg
     4261 gtcccggtat ccagccaggc gaagccccgg cccagggtgg tgacgtgcag atgtcgcagg
     4321 ttaaggtaat gttcgtttac cgtggtgatt tccagttctc cgcgtgccga tggttttaca
     4381 gctgcagcca catccagcac gttgttgggg taaaagtaca gaccggtgac ggccaggttt
     4441 gaccttggct tttgtggttt ttcctggatg gaaaccggaa ttcctgccgg gccgagttct
     4501 accaccccgt agcgatgcgg gtctttaatg gggtatccaa aaacatgcgc aaatccgtta
     4561 tttgccgagg cgacggccct ttggagcatg gcccggaagc cctgcccgta gaagatgttg
     4621 tcgcccagga ccaggcacac gttgtcgttg cctgtgaaat ccctgccaat gatcagtgcc
     4681 tgagccagtc cttcgggccg gggttgcggc gcatattgga agcgtacccc aaaggcttca
     4741 cccgtgccca gcaggcgctc gaaggagggc aggtcctgcg gtgtggatat gatcaggata
     4801 tcccggatcc cggccagcat caggacagac aacggatagt agatcatggg tttatcgtaa
     4861 acgggaagca attgcttgga aactcctttt gtgatgggat acaggcgtgt gcctgaaccc
     4921 ccggccagaa ttattccttt catatccggt ttttgttgaa taggctcaaa gttaataatt
     4981 atctttgagg gatgaaacgt ttgttgcagc tgataatcct tcttttactg caggccgggg
     5041 ctttgcccgc ggcctcccaa caacggccgg ctcaggtgca gcaggccccc aagccctcgg
     5101 gagcacagca ggttctgcag gggccgcagg ctcaggagcc gctcgtagca cagcaggttc
     5161 tgcaggggct gcaggctcag
//
LOCUS       DAOSOF010000131         5133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16365_length_5133_cov_4.772351, whole genome shotgun sequence.
ACCESSION   DAOSOF010000131 DAOSOF010000000
VERSION     DAOSOF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16365_length_5133_cov_4.772351"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..97)
                     /locus_tag="PLM68_07290"
                     /pseudo
     CDS             complement(1..97)
                     /locus_tag="PLM68_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
     gene            379..780
                     /locus_tag="PLM68_07295"
     CDS             379..780
                     /locus_tag="PLM68_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55198.1"
                     /translation="MSDFDITTRTSSPRIDRLGPGEIFVFGSNLSGAHGGGAALLAVN
                     KFGAVWGQGVGLQGQSYAIPTMHGGPDAIKPYVDEFIDFARRHPELTFLVTEIGCGIA
                     GFTPSQIAPLFAGAKDIHNIHLPARFWAELR"
     gene            897..1700
                     /gene="zupT"
                     /locus_tag="PLM68_07300"
     CDS             897..1700
                     /gene="zupT"
                     /locus_tag="PLM68_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT55199.1"
                     /translation="MDSRILLPFLLSLFAGLATLIGFGITFIAKRTNRKLLSYALGLS
                     AGVMIYVAFVEIFAEARSILIADMGPRHGLLMTVIFFFGGMALIALIDRFVPSFGNPH
                     EPHSVEEMAKKGHKKPKLMKMGVMTAVAITIHNFPEGIATFMAAIDNSTLGITIATAV
                     AIHNIPEGIAVSIPIFYATGSRKKAFCFTLVSGLAEPLGALLTYLILMPFLSPVVMGC
                     VFAVVAGIMVYISVDELLPSAHEYGEHHIAIYGLISGMAIMAISLILFA"
     gene            1813..2793
                     /locus_tag="PLM68_07305"
     CDS             1813..2793
                     /locus_tag="PLM68_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT55200.1"
                     /translation="MKSARHYFFLTFTIMLNQDQYTATDKMGDLICDNYPMLFVMSRF
                     GISHGFGEKTIEEVCRQNNVHTNTFLAVVNMLISGKRDENAEPSLPSLLDYLHNSHSH
                     FLDIRLPAIRKKLLQTIGTPHDGVAKAVIRYYDEYVEQVDTHMAYEEQTVFPYVRSLL
                     KGELSGDYCIRVFCRQHDNIESKLSELKNILIKYYPTGNPHELNNVLFDIFACARDLA
                     SHNFIEDELFVPMISQLEQDMRPDRKTLPKECRKQTAKEASTGKELSKREKEIIAGVA
                     KGLTNKQIAEKLFISVHTVMTHRKNIAGKLQIHSPSGLTIYAVLNNLVEL"
     gene            2918..4912
                     /locus_tag="PLM68_07310"
     CDS             2918..4912
                     /locus_tag="PLM68_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOT55201.1"
                     /translation="MKHTRQPLKAFTLSLATFTFAFGLALLTCSCKRDIPPFSIGADM
                     SFIPQYESRGMAFSDTTGQEQDVLAIMAANGFDNIRLRIFVDPQAPGGYSAEGFCNLE
                     QTVAMAQRIVAAGMTFSLDFHYSDYWADPDKQYKPSAWNGLTGEALENALYEYTHHVL
                     TTLKEAGAAPQIIQPGNEINHGMVWPEGYIDDNAREENWAALMGLYKAAVKAAREVLP
                     ESRLMVHLALGGQNILCREFLDIMNRYGAEFDLIGLSYYEQWHETYDDLQANLYDLAA
                     RYDKPLYVCEYGANPDNIRRINDIVRSVPGGLGAGTMAWEPARVLFPMGKADPAILDI
                     YRDMAATYKKRAPREMPLPRRELTFDRPIVGADISWVPEQEDKGTVFSDNGVEKDVME
                     ILKDNGFNWVRLRLFVDPAAENGYSPQGFCGLEKTLEMARRAKNAGLKLLLNFHYSDT
                     WADPGKQYTPSSWAQHAGSGLEGQVYRYTYEVIRRFMEEGVCPDMVQTGNEINHGMLW
                     PQGKIEDSYMHFGVLLRCATAAVRAANPSIPVMVHIACGGQNDESVYFLDKILSRDVK
                     FDIIGQSYYPQWHGTLEDLQHNLNDLAARYNKPVIVVEYQEHRLEVNRIVRDIPGEKG
                     LGTFIWEATSPRWGNLFDEKGATNENMKLYPQFLESYDFL"
     gene            complement(4897..>5133)
                     /locus_tag="PLM68_07315"
     CDS             complement(4897..>5133)
                     /locus_tag="PLM68_07315"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HOT55202.1"
                     /translation="TPVQTVKKLTYRQRKRLEELEAEIQALEEEKFRLESLLSDPERP
                     LSQITEASVRMGEILARLELDWEELLGLEDQRKS"
BASE COUNT         1256 a         1504 c         1233 g         1140 t
ORIGIN      
        1 ggtcattttc cttatcgtag aagaggcctt tggcatactg tgtttcattg gagaagcggc
       61 caatggcaac tttacgcttg atcgttttgt tgctttgcgc cgacaccgga aaagccacca
      121 gcacagccaa taacgccggc aataaaaatg tgaggattct tttcatgttg ttttgaattt
      181 ttaggtggta aaaataacat aaaaaactca atcacctgcc atttctgttt tatgtatatt
      241 tgctaaccgg cagtattgct aaacccgtaa ctactgaacc attgcttaac tcctgaacca
      301 tgaagtaact gttcaaaaat caagcaacag ctaaactata aagtaactcc tgaaccatca
      361 actaacacct ataacatcat gagcgacttt gacataacca cccgcacttc ttccccccgc
      421 atagaccggc tcgggccagg cgagatcttt gtctttggca gtaacctttc cggagcccac
      481 ggcggaggag ccgccttgct ggcagtgaac aaattcggag ccgtatgggg ccagggcgtc
      541 ggccttcagg gccagtcata cgccatcccc accatgcacg gcggccccga tgccatcaaa
      601 ccctacgtgg acgagttcat cgatttcgcc agacgccacc ccgaactcac attcctggtc
      661 accgaaatcg gctgcggcat cgccggcttt accccctccc aaattgctcc cctttttgcc
      721 ggggccaaag acatccacaa catccacctc cccgcccgct tctgggccga gctgcggtga
      781 tgcgctgagg tttctacctg ttgtgccctg ccttgcagaa taccttaatc tgggtatttt
      841 cagacccgtc tcctatgcct atctttgcag ctgttaagac tttccgcact ttactcatgg
      901 attcaagaat tctgctaccc tttcttctct cgctctttgc aggcctggcc acactcatag
      961 gctttggcat caccttcata gccaaacgca ccaaccgcaa actcttatcc tatgccctgg
     1021 gcctttccgc cggggtcatg atctatgtgg cttttgtcga gatttttgcc gaggcacgct
     1081 ccattcttat cgccgatatg ggtccacggc atggcctcct gatgaccgtc atcttctttt
     1141 ttggaggcat ggccctcatc gccctgattg accggtttgt gccatccttc ggaaatcccc
     1201 acgagcccca ctccgtcgag gagatggcca aaaagggcca taaaaaaccc aaactcatga
     1261 aaatgggggt catgacagcc gtagccatta ccatacataa cttccccgaa ggaattgcca
     1321 ctttcatggc agccattgac aattccaccc tggggatcac cattgccaca gccgttgcca
     1381 tccacaacat cccggaaggg attgccgttt ccatccccat tttttatgcg accgggagcc
     1441 gcaaaaaagc cttctgcttt acccttgtct ctggacttgc cgagcccctg ggcgccttgc
     1501 tcacctacct catcctcatg cccttcctct cccctgttgt catgggatgc gtctttgcag
     1561 tggtagcggg catcatggtg tatatttcgg tggacgagct cctgccctct gcacacgaat
     1621 acggcgaaca ccacatagcc atctacggcc tcatctcggg catggccatc atggccatca
     1681 gcctcatact gttcgcatag gagcagaagg agtgccgcgc actcttgatg tattgatttg
     1741 tgtagtaagt agttaacaaa agagctctcc gggagagctt ttttgttatg tggttgatag
     1801 ctaacaagat gaatgaagag tgcgcggcac tatttttttc ttacctttac aatcatgctc
     1861 aaccaggacc aatatacggc aacggacaag atgggtgacc tgatctgcga caattatccc
     1921 atgctttttg tcatgagccg ctttggcata tcccacggct ttggtgaaaa gaccatagaa
     1981 gaagtatgcc ggcagaacaa cgtgcacacc aatacctttc tggccgtagt caatatgctt
     2041 attagcggca aacgggacga aaacgcggaa ccctccctgc catccctgct tgactacctg
     2101 cacaattcac acagccattt cctggacatc cgcctgcccg cgatcaggaa aaagctcctc
     2161 cagaccatag gtacgcccca tgacggcgtg gcaaaagccg ttatccgtta ctatgatgaa
     2221 tacgtagaac aggtagacac tcatatggcc tatgaagaac aaaccgtttt cccctacgtg
     2281 cgctcgctgc tcaaaggcga actaagcggc gattactgca tccgcgtctt ttgcaggcag
     2341 cacgacaaca ttgaatccaa attgtccgag cttaaaaaca tcctgatcaa atactatccc
     2401 acagggaacc cccacgaact gaacaatgtt ctttttgaca tatttgcctg tgcccgggac
     2461 ctggcttcac acaattttat tgaagacgag ctgttcgttc ccatgatctc gcagctggaa
     2521 caggacatgc ggccagaccg gaaaactctc ccaaaagaat gccggaagca gacagccaaa
     2581 gaagcgtcca ccggcaaaga actgagcaag cgggaaaaag agatcattgc cggggtggcc
     2641 aaagggctca ccaacaaaca aatcgcggaa aaactcttta tctcggtaca taccgtcatg
     2701 acccaccgaa agaacatcgc cggcaagctc cagatccact caccctccgg cctgaccatc
     2761 tacgccgtcc tcaacaacct ggtggaattg tgattttttt acctaagccg aggcgttaac
     2821 ctacagcatg aacctgtaac cagaaccttt aacttgaacc tgtaaccgga acctgtaact
     2881 tgaacctgct tcctgagtaa acaataactt aaatatcatg aaacacaccc ggcaacccct
     2941 taaggcattc accctgagcc ttgcaacgtt cactttcgca tttggactcg cgttattaac
     3001 ctgcagctgc aagagggaca ttccgccctt cagtatcggg gccgatatgt ccttcatccc
     3061 gcaatacgaa tcgcggggga tggcgttttc agacactacg ggccaggagc aggatgtgct
     3121 ggccatcatg gcggccaacg gctttgacaa catccgcctg cggatcttcg tcgatcccca
     3181 ggctccggga ggttactcgg cagaaggctt ctgcaatctg gaacaaacgg tggccatggc
     3241 ccaacgcatc gttgccgccg gcatgacctt ctcgctggat ttccactata gtgattattg
     3301 ggcagatccg gacaagcagt acaaaccctc ggcctggaac ggcctgacgg gcgaagcgct
     3361 ggaaaacgca ttgtatgaat acacgcatca tgtgctgacc acgctgaagg aagcaggcgc
     3421 tgccccgcag atcatccagc cgggcaacga aatcaaccac ggcatggtct ggccggaagg
     3481 atatattgac gataacgcca gggaagaaaa ctgggctgcc ctgatgggtc tgtacaaagc
     3541 cgcagtaaaa gccgcccggg aagtcctgcc ggaatcccgc ctgatggtcc acctggccct
     3601 tggaggacaa aacatcctct gccgggaatt cctggacatc atgaaccggt acggggccga
     3661 gtttgatctt atcgggctat cctactacga acaatggcat gaaacctacg acgacctgca
     3721 agccaacctg tacgatctgg ctgcccgtta cgacaaaccc ctgtatgtct gtgaatacgg
     3781 tgcaaacccg gataacatca ggcgtataaa cgatatcgtc cgatccgtgc cgggtggcct
     3841 gggagccggc accatggcct gggaacctgc ccgggtactc ttcccaatgg gaaaggctga
     3901 tcccgccata ttggacatat accgcgacat ggctgccacc tacaaaaaaa gagctcccag
     3961 ggagatgccc ctgccccggc gcgagctgac ctttgaccgg cccatcgtag gtgccgacat
     4021 atcgtgggtt cccgaacagg aagacaaggg taccgtattc tcggacaacg gagtggaaaa
     4081 ggacgtcatg gaaatcctga aggacaacgg tttcaactgg gtccgcctgc ggcttttcgt
     4141 agacccggcg gctgaaaacg ggtattcccc ccagggcttc tgcgggttgg aaaagacgct
     4201 ggaaatggcc agacgcgcca aaaacgcagg cctgaaactg ctgctcaact tccattacag
     4261 cgacacctgg gccgatccag gcaagcaata caccccatca tcctgggctc aacacgccgg
     4321 ctcgggactg gaaggacagg tctaccgcta cacctatgag gtcatcaggc ggttcatgga
     4381 agaaggagtc tgccccgaca tggtccagac cgggaacgag atcaaccacg gaatgctatg
     4441 gccgcagggc aaaattgaag attcctacat gcacttcggc gtattgctgc gctgtgccac
     4501 ggccgccgtg cgtgccgcca atccttccat tcccgttatg gtgcatattg cctgcggggg
     4561 acaaaatgac gaatcggtct atttccttga taagatcctg agccgcgacg tgaagttcga
     4621 tatcatcggg cagtcctact atccccaatg gcacggcacc ctggaagacc tgcagcacaa
     4681 cctgaacgac ctggcagcca ggtacaacaa gcccgtgatc gtggtggaat accaggagca
     4741 ccgcctggaa gtgaaccgga tagtacgcga cataccgggt gaaaaggggc tcggaacctt
     4801 catctgggaa gccacttcgc cacgctgggg caacctgttc gacgaaaagg gcgccaccaa
     4861 tgaaaacatg aagctctacc cgcaattcct ggaaagttac gatttcctct gatcttccag
     4921 gccgaggagt tcttcccaat ccagctccag ccgggcaagg atctcgccca tccttaccga
     4981 ggcttccgtg atctgtgaca ggggcctttc cgggtcggag agcaggcttt ccaaacggaa
     5041 tttttcttct tcgagcgcct ggatctccgc ctccagttct tccaggcgtt tccgctgcct
     5101 gtacgtcagc ttcttaacag tctggacggg ggt
//
LOCUS       DAOSOF010000132         5132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16369_length_5132_cov_6.346661, whole genome shotgun sequence.
ACCESSION   DAOSOF010000132 DAOSOF010000000
VERSION     DAOSOF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16369_length_5132_cov_6.346661"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..547)
                     /locus_tag="PLM68_07320"
     CDS             complement(143..547)
                     /locus_tag="PLM68_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT55203.1"
                     /translation="MNQNEIIAPCGMNCQLCYGYVRKKNRCNGCLRMNNANEPDVWRK
                     CSIVHCEDRAKTASGFCYECTRYPCRRLKDLDKRYTTKYGMSMLDNLLFIKEKGLEEF
                     VRAEQEKWKCRNCGAGLSVHRDFCLQCGNKYR"
     gene            720..1070
                     /locus_tag="PLM68_07325"
     CDS             720..1070
                     /locus_tag="PLM68_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55204.1"
                     /translation="MRKIFCVFLFSLLLGINSLNAQQIRAFEFEVGGLLLFPNGTHVQ
                     YSKKGPGWGIYLEPRINIGNTGFDIAAQFSYASIRRSNPKNDLYGHSMHSHTNMYPLI
                     DFVITPGEIFMPIK"
     gene            complement(1337..1660)
                     /locus_tag="PLM68_07330"
     CDS             complement(1337..1660)
                     /locus_tag="PLM68_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOT55205.1"
                     /translation="MNWIIIVIAGFFEAAFAFCLGKARMTTGTEMYLWYVGFVVALCI
                     SMVLLVKATQTLPIGTAYAVWTGIGAVGTVLAGIFIFKEPVTFSRIFFLSTLIISIIG
                     LKVVS"
     assembly_gap    2196..2295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2403..2492)
                     /locus_tag="PLM68_07335"
     tRNA            complement(2403..2492)
                     /locus_tag="PLM68_07335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2456..2458),aa:Ser,seq:cga)
     gene            complement(2698..3324)
                     /locus_tag="PLM68_07340"
     CDS             complement(2698..3324)
                     /locus_tag="PLM68_07340"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOT55206.1"
                     /translation="MGKRKLSRGKILAIIITSILVLDQISKIWIKTHFALHESVNVLG
                     KWFQLYFVENDGMAFGMVFGGDSGKLILSLFRVVLSIVIMWYICKLLKKPDTPMGVLV
                     GLSMVFVGAIGNIIDCAFYGLIFNESGVTEVATLFPPEGGYGTFLHGKVVDMLYFPLI
                     DTVLPENFPIWGGRRFVFFQFIFNLADSAITCGALYLLIFKYKFFSKK"
     gene            complement(3334..3756)
                     /locus_tag="PLM68_07345"
     CDS             complement(3334..3756)
                     /locus_tag="PLM68_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOT55207.1"
                     /translation="MTTKKSKTDEKVTEEVREKVRYSDQELQEFKTLINEKLAQARAD
                     YEELKAIVTHSLSNDTEDTSPTFKVLEEGASALSKEEAGQLALRQHKFIQSLEMALIR
                     IENKTYGICRETGKLIPKERLRLVPHATLSVEAKNNRE"
     gene            complement(3794..>5132)
                     /locus_tag="PLM68_07350"
     CDS             complement(3794..>5132)
                     /locus_tag="PLM68_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOT55208.1"
                     /translation="PFKMLDMYGADPLRWYMLTNAQPWDNLRFDETGVDEIRRKFFGT
                     LYNTYSFFALYANVDGFTADLAREDNPGWVPVKERSEMDRWIISLLNTLTANVLDAYE
                     NYDITTAGRLIQDFTGDHLSNWYVRLNRKRFWRGELDDDKRAAYQTLYTCLRTIALLG
                     APIAPVYMERLYMDLGAGDSVHLELMPKVQDELIDKALEERMDLAQRCTSMVLGLRRK
                     VNIKVRQPLSKIMIPVLDKSMGKKLAKVKSIILNEVNVKELEFIHDTAGLILKGIKPN
                     FKVLGKKCGPRMKEIASALNAFSQEEIALVEKAMETGSYTLSLDGGEVLLEREDVTIT
                     SQDMPGWLIAIEGPLTIALDVTITDALLREGTARELINRIQNLRKDTGLEVTDKIRLV
                     IEERKDVADALQDFRQFIASQVLATDISMAATPTAAHAVEWKEGTLSIHIEKS"
BASE COUNT         1223 a         1236 c         1147 g         1426 t
ORIGIN      
        1 tgccgagcca ttccctgaac tcatcccggc tttcaaacaa catttcctca ttcatatctg
       61 aaagactgat ttttactata ctttaactgt tatacctcaa aaaccagcac tgtgcctgca
      121 caggagctgc aaatcttttg cctcatctgt atttattgcc gcattgcagg caaaaatccc
      181 tgtgaacact taatcctgca ccacaattcc tgcatttcca cttttcctgc tctgccctta
      241 caaattcttc aagccctttt tccttgataa agagcagatt atccagcatg ctcatcccat
      301 attttgtggt atagcgtttg tctagatctt tgagccgcct gcagggatac cgggtgcact
      361 catagcaaaa tcccgaggca gtcttggccc ggtcttcaca atggactatg gaacatttcc
      421 tccatacatc cggttcattc gcattattca ttctaaggca tccgttgcag cgatttttct
      481 tacgcacgta accgtagcat agctggcaat tcatcccgca gggagcaatt atttcgttct
      541 gattcattct tgttgacatt ttttattcaa tttctattgg aaccgacgtt cttttggata
      601 cctcctcaag gctcatcccc tggttgcaga aaataatgct acaaaatatg atttttaatt
      661 gaatatcttg tgttttatat tttcattttt ggctaatttg catctcaatt catgatatta
      721 tgagaaaaat attttgcgtc tttttatttt cgttgctttt agggattaat tctctaaatg
      781 cccagcaaat ccgggcattt gaatttgaag ttggagggct cttgcttttc cctaatggaa
      841 cacatgttca atacagcaaa aaaggcccgg gatggggaat ttatcttgaa ccacggatca
      901 atatcggcaa tacaggattt gacattgccg cacagttttc atatgcttca attcgtagaa
      961 gtaatccaaa aaatgattta tatggacaca gtatgcactc ccataccaat atgtacccac
     1021 tcattgattt tgtgattact cctggagaaa tcttcatgcc cataaaatag tgcctttcgt
     1081 gggattaggc gtaggaacgg tgggaggttt tcttgtgtct gccgatcaat ggtatgaagt
     1141 atttggcgat acaggagaac ctgagggtta cacagatgaa agattctcct atgttttatg
     1201 tcctcgtatt ggcgtagagg tttgcaatag aattcgtttt acagtagatt acaagatgac
     1261 aaatcagatt gcaaaccata tctgtgcaaa cctgagtttg tcactgggaa ttgtgtttgg
     1321 cggaggtgtt cctcccctac gaaaccactt tcaatccaat aattgatatg atcaatgttg
     1381 ataagaaaaa aattcttgaa aatgtaaccg gctctttaaa gataaaaata cctgccaata
     1441 cggttccaac agctccaatc ccggtccaca ctgcataagc ggttccaatc ggtaacgttt
     1501 gagtagcctt aaccagaagc accatactta tgcatagtgc gacaacaaat cccacatacc
     1561 ataagtacat ttcagtacct gttgtcattc ttgctttccc aaggcagaat gcaaaggctg
     1621 cctcaaaaaa tcctgcaatt actataatta tccagttcat actacaattt ctactacgtg
     1681 tttcaatttg agttgcaaag atcgaaaaaa ccacagacag gtggaacctc cttgctgttt
     1741 tttgccgagg cttcccgatc ttgtggtggc agcgacattg tcattagtcc gtattgctgc
     1801 accaacgacc gcttggtggt ctacaaggcg gtgcgccaaa ggcttttggc gcttaaaata
     1861 tgctgcttcg cagcaccaaa caaaaggagg tggaacctcc cgcgccattt tagatatgga
     1921 aaatttatat ataacagtaa tacagtgaat taagaaataa ctattccaaa tttggtgcag
     1981 gaggttccac ttccttgctt ctgcccgttg gaggtagctg gcagcaaggc tgccctcggg
     2041 attaccttcg cgctaaagcg ctcggtgttc ccttggggga ggcttcagtt ccaaaaacgc
     2101 aaagcagcaa ggctgccctc gggattacct tcgcgcctgc agcgctcggt gttcccttgg
     2161 gggaggcttc agttccaaaa acgcaaagca gcaagnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnngcaca gctttttatg gcagcaggcc gcgaaaaaca agctggcttg
     2341 tttttcggac ctgctgtcat aaaaagacac ggacattgtc cgtgtctttg cgtttttgga
     2401 actggcggag agagagggat tcgaaccccc ggaacccaga tgggttcaac ggttttcgag
     2461 accgccccgt tcgaccactc cggcatctct ccattcattt tcgggtgcta aaccgaaggc
     2521 ttgtgtcagg cagtttgctg cgctacgctt atgggctttt gcaatgcccg ccgttcagta
     2581 gctctgcctt tcagcatctt gccgcgtccg ccgttcagta gctctgcctt tcagcatctt
     2641 gcagcgcccg ccgttcggca gtcctcccgt aagggttggc aaaggtaggg aatcttttta
     2701 ttttttagag aaaaatttgt acttgaaaat caagagatag agtgcgccgc aggtgatggc
     2761 agaatcagcc aagttgaata tgaactggaa aaacacaaag cggcggcctc cccatatggg
     2821 gaaattttcg ggcaggacag tgtctatgag cgggaagtaa agcatgtcca cgactttgcc
     2881 gtggaggaag gtgccataac cgccttcggg aggaaagagg gtggctactt cggtcacgcc
     2941 cgattcattg aagatcagtc catagaaagc acagtcaatg atatttccaa tagcccctac
     3001 aaaaaccatg gacagcccga ccaggacgcc catgggagta tccggctttt tgagcaattt
     3061 gcatatgtac cacatgatga caatgctcag caccacccgg aacaggctaa ggatcagttt
     3121 cccgctgtca cctccgaaga ccatgccaaa ggccatgcca tcgttttcca caaaatataa
     3181 ctggaaccat ttacccaata cgttgacgct ttcgtgcagc gcgaaatggg tttttatcca
     3241 gatcttggat atctgatcca aaaccaggat tgaagtgatg attatcgcca gtatctttcc
     3301 cctggataac tttcttttcc ccataagacc gtactactca cggttattct tcgcctcaac
     3361 ggatagggtg gcatgtggaa ccaggcgaag gcgctccttg gggatgagtt tgccggtttc
     3421 acgacatatc ccgtaggtct tgttttcgat cctgatcaag gccatttcca gggattggat
     3481 gaatttatgt tgacgcaaag ccaattgtcc cgcctcttct ttggaaagtg ccgaggctcc
     3541 ttcttccaac actttgaatg tgggggaagt atcttccgtg tcgttgctca gactatgggt
     3601 gactattgct ttaagttcct cataatctgc ccgtgcctgg gccagttttt cgttgatcag
     3661 ggtcttgaat tcctggagct cctggtcgga atagcgaacc ttttccctta cctcttctgt
     3721 aaccttttca tcggttttgc tttttttagt agtcatggtt ctttttttta ggaaaaacaa
     3781 agttaatatt aaattatgat ttctcaatat gaatggacag ggtaccttct ttccattcaa
     3841 cagcgtgagc tgcagtgggt gtggcagcca tggatatatc cgtggcaagc acctggctgg
     3901 caatgaattg cctgaaatcc tgcagcgcat ctgccacatc tttccgctct tcgatcacca
     3961 ggcgtatctt gtccgtcact tccagtcccg tgtcctttct caggttctga atcctgttga
     4021 tcagttcccg ggccgtaccc tcacgcagca acgcgtcggt gatggtcaca tccagggcga
     4081 tggtcagtgg tccctcaatg gcaattagcc atccgggcat atcctgcgag gtaatggtta
     4141 cgtcttcccg ctccagcagc acctctcccc catccaggga taaggtgtag cttcccgttt
     4201 ccatcgcttt ttccaccagg gcaatttctt cctggctgaa agcgttgagt gccgaggcga
     4261 tctctttcat ccggggaccg cacttcttgc ccagcacctt gaagttcggc ttgattccct
     4321 tgaggattaa tcctgccgtg tcgtgaatga actcgagttc cttgacattg acttcgttca
     4381 agatgatgga tttgaccttt gcgagctttt ttcccatgga tttgtccaac acaggaatca
     4441 tgatcttgct caggggctgc cgtaccttga tgttgacttt gcggcgcagg cccagcacca
     4501 tggaagtgca gcgctgtgcc aggtccatgc gttcctcaag ggctttgtca atgagttcgt
     4561 cctgtacctt gggcattaat tccaggtgca cggaatcacc ggcacccagg tccatgtaca
     4621 aacgttccat atagacgggg gcaatgggtg ctcccagcag ggctatggtc cgcaggcagg
     4681 tatacagggt ctggtaggca gcgcgtttgt catcatccag ttcaccgcgc cagaaacgct
     4741 tacggttcag acgtacgtac cagttgctca ggtggtcccc agtaaagtcc tggataagac
     4801 ggcctgccgt tgtgatatcg taattctcgt aggcatcgag aacattagct gtgagtgtgt
     4861 tgagcaaaga aatgatccag cggtccatct cggatcgctc ctttacaggc acccatccgg
     4921 gattgtcttc ccgggcaaga tctgccgtaa agccgtccac gttggcatac agcgcaaaga
     4981 acgaataggt gttgtacaac gttccgaaga atttgcgacg gatttcatcc acgcccgttt
     5041 cgtcaaaacg caggttgtcc cagggttgtg cgttggtcag catgtaccag cgcaaaggat
     5101 cggctccgta catgtccagc attttgaagg ga
//
LOCUS       DAOSOF010000133         5128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16388_length_5128_cov_5.261384, whole genome shotgun sequence.
ACCESSION   DAOSOF010000133 DAOSOF010000000
VERSION     DAOSOF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16388_length_5128_cov_5.261384"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PLM68_07355"
     CDS             complement(<1..1769)
                     /locus_tag="PLM68_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT55209.1"
                     /translation="MKETTKKKINKWLWIIVLIPVAGFFLMLFLVGVFTNIPSFRVLE
                     NPQTNLATELISEDGVVIATFHIENRSYVQYEDLSESLKNAVLATEDIRFYRHSGIDF
                     RALARVAVKSLLLGQSSSGGGSTLTQQVAKTLYPRDTSTYDFPGGAAFKMLVIKMKEW
                     ITAVKLERNYTKEELLTMYLNSIFFGSNAYGIRTAAATFFDKHPSQLTVEEAATLVGM
                     VNKPTRYNPVLNYESSLGRRNHVISQMRKYDFISRQEADSIMALPIVLNYTTQDHNAG
                     LAPYFRDMLRRVMSAGKPDPADYAYEEHYQEDLDLWENDPLYGWLNKNTKPDGTRYDL
                     DRDGLKIYTTINARMQLYANLAVEEHLATYLQPTFNREIRYKQHKPFSDDVSAEIRDL
                     VMKQAIRWSDRYRNMKTDGATDAEIDLAFRTKTPMRVFSWTGKDGTYATDTLLSPRDS
                     ILHYKSILRASIMAMEPETGHVKAYVGGPNYRYFKYDNARQGRRQVGSTIKPFLYSLA
                     MQEGWTPCDQVVNVPQTFILSDTTWTPESVDKPEWIGRKVTLKWGLTKSSNNISAYLM
                     KQFGPQAMVAMCHKLGIKSFLDPV"
     gene            1863..4157
                     /gene="topA"
                     /locus_tag="PLM68_07360"
     CDS             1863..4157
                     /gene="topA"
                     /locus_tag="PLM68_07360"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT55210.1"
                     /translation="MEKNLVIVESPAKAKTIAKALGDGYLVTSSFGHIRDLSKKKIGI
                     DYENGFTPDYVIPPDKKDLVKKLTKAAAEASVVWLASDEDREGEAIAWHLKETLQIPG
                     EKIRRIAFHEITKNAILEAVENPRDIDMNLVMAQQARRVLDRLVGYEVSPVLWRKVKP
                     RLSAGRVQSVALRLVVEREREILQFKATRSFRVEGLFTCPARNSEPFRAELDTRFNTE
                     EQAREFLLSCIGADHTVTGIDRKKGQKSPAPPFTTSTLQQEASRKLGFSVSQTMSVAQ
                     RLYESGHITYMRTDSTQLSKMALALAKEIITGTFGAGYSKTRQYATRSKGAQEAHEAI
                     RPTFMDKVTVEGTATDKKLYELIRKRALASQMADAQIDKTQITISCGNTGKQFMATGE
                     VVVFEGFLKMYREGSDEDPEKNNGKPTLALPFLETGDNLNASQIRAIERFTQSPYRYT
                     EASLVKKLEELGIGRPSTYAPTISTIIERQYVVKGDRPAKKRNYLELRLEGNQVTREE
                     CSENYGEERKKLFPEDIGILVNDFLMEHFHDIVDYNFTAKVEEDFDRIASGKLVWNKM
                     LDNFYKPFRKTLDKALETSHPGKSERLLGNDPVTGKPVTVRLGRYGAMAQLGASDDPD
                     KRYAGLQKGQLLESITLEEALRLFTLPRDLGVYQDFPVVVSTGRFGPYVKWQGKFISL
                     AKTDDPYTVTLQRSIQLIEQSKTEDSKKHILEFPEEGIRILKGRYGPYITQNKKNYKI
                     PRGTDPESLTLEDCMKIIQNKNNE"
     gene            4150..>5128
                     /locus_tag="PLM68_07365"
     CDS             4150..>5128
                     /locus_tag="PLM68_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55211.1"
                     /translation="MSEKHFPLEHLHADALCSEMPSCMAILVFTGKGGAVLDALQCLY
                     PQSHSRIYYMEDTMQTQLPAILSAVEKLHLPCIVMHPGDIPGMGAFLPPDARVSLVSQ
                     GTENPDHVTGLLEKNTPGYFTWIGFQRYLTDPAVMEKLSGHFMETLRLGEFRKKITSA
                     EPLLREATHHFTDMRCVRHADMPDMKEGNPNGLYAEEICTLGRYMGLSHHFQLAVVYG
                     YPSDCRKGSLTAKLNAQFIWHLTEGLAVSVEEHPAEESVSFQRKAVQMGEDGQELVFL
                     QSRKSGRWWMEVTNCKQDGHPFYISCSMDDYVMACKGEIPLKWLFYYQKS"
BASE COUNT         1299 a         1299 c         1366 g         1164 t
ORIGIN      
        1 accgggtcaa ggaaactctt gatgcccagc ttatggcaca tggccaccat ggcctgggga
       61 ccgaactgtt tcatcaggta agccgagatg ttgttgctgc ttttggtcaa cccccacttg
      121 agggtcacct tcctcccgat ccattccggc ttgtccacgc tttcgggggt ccaggtggta
      181 tcggacaata tgaaggtctg gggaacgttg accacctggt cgcagggcgt ccagccttcc
      241 tgcattgcca gggaatacag gaaaggcttg atggtcgaac ccacctgccg tcttccctgg
      301 cgggcgttgt cgtacttgaa ataccggtaa ttgggtcctc ccacatacgc tttcacatgg
      361 cccgtttcag gttccatggc catgatggag gcacgcagga tggacttgta atgcaggatg
      421 gaatcacgcg gggatagcag cgtatctgtc gcatacgtcc catcctttcc tgtccatgaa
      481 aacactctca tgggggtctt tgtcctgaat gccaggtcaa tttccgcatc ggtggccccg
      541 tcggttttca tattacggta ccggtcgctc caccgtattg cctgtttcat tacaaggtcg
      601 cgtatctctg ccgaaacgtc gtccgagaag ggcttgtgtt gcttgtacct gatctccctg
      661 ttgaatgtgg gctggaggta tgtggccagg tgttcctcca ctgccaggtt ggcatagagt
      721 tgcatccttg cattgatggt ggtatagatc ttgagaccgt cacggtccag gtcgtacctg
      781 gtcccgtccg gttttgtgtt tttattcagc cacccgtata acgggtcatt ttcccataag
      841 tccaggtctt cctggtagtg ttcctcatag gcataatccg ccggatccgg cttgcccgcg
      901 ctcatcacgc gcctgagcat gtccctgaaa tagggggcca ggccggcatt gtggtcctgt
      961 gtggtataat tcagcacgat aggcagtgcc atgatggagt cggcctcctg tcgggaaatg
     1021 aaatcgtatt tgcgcatctg ggatatgacg tgattgcgcc tgcccagaga agattcataa
     1081 ttcaggacag gattgtaccg ggtcggcttg ttgaccattc ccaccagggt tgcggcttct
     1141 tcaacggtaa gctgggaagg gtgcttgtca aaaaaggtgg cggcagccgt ccggatacca
     1201 taggcattgc tcccgaagaa aatcgagttc aggtacatgg taagcaattc ttccttggtg
     1261 tagttgcgtt ccagcttgac agccgtgatc cattctttca ttttgatcac cagcatcttg
     1321 aaggcggcac cgcccggaaa gtcatacgtc gaggtgtccc gaggatacag tgtcttggct
     1381 acctgctggg taagggtact gcctccccct gaactgcttt gccccaataa taaagacttc
     1441 acggccaccc gcgccagggc gcggaagtca atccccgaat ggcggtaaaa cctgatgtct
     1501 tccgttgcca gaacagcgtt cttcagggat tcggacaagt cttcgtattg tacataggag
     1561 cggttctcta tgtggaatgt ggctatgaca accccatctt cggatatcag ctcggtagcc
     1621 aggttggttt ggggattttc caggacacgg aacgagggaa tgttggtgaa cacccccacc
     1681 aggaacagca tgaggaagaa tcctgcgacc ggaatcaaaa ctatgatcca caaccacttg
     1741 ttaatttttt tctttgtcgt ttccttcata acagaacagt gtagctctgc aaaattacag
     1801 ataaatattt ggaatatacc ttattttatg gcttactttg caccgtctga ttttgaatga
     1861 gtatggagaa gaacctggtt attgttgaat cgcctgccaa ggcaaaaacc attgccaagg
     1921 cgctgggaga tggatatctg gttacatcga gttttggtca cataagggat ctgtcaaaga
     1981 aaaagatcgg gattgattat gaaaacgggt ttacccccga ttacgtcatc cctcccgaca
     2041 agaaagacct tgtgaaaaaa ctcaccaaag ctgctgccga agcatcggtc gtgtggcttg
     2101 catcggatga ggaccgtgaa ggcgaggcca ttgcatggca tctgaaggaa actttgcaga
     2161 taccgggaga aaagatacgg cgcatagcct ttcatgaaat cacaaaaaac gccatcctgg
     2221 aggccgttga gaatccaagg gatatcgata tgaacctggt gatggcgcag caggcgcggc
     2281 gtgtcctgga ccgccttgtg ggttacgagg tttccccggt cttgtggaga aaagtcaagc
     2341 ccaggctgtc cgcaggaagg gtccagtctg ttgctctccg ccttgtggtg gagcgtgagc
     2401 gggagatcct gcagttcaag gcaacccgtt cgttccgggt ggaaggactc ttcacctgcc
     2461 cggcaagaaa ttcggaacct ttcagggcag agctggacac ccgtttcaac acggaagagc
     2521 aggcccgcga gttccttctt tcctgcatcg gcgcggatca tactgttaca ggcattgaca
     2581 ggaaaaaggg ccagaaaagc cctgcccctc cctttaccac ctcaaccctg cagcaggaag
     2641 cttcccgcaa actgggattt tcggtgagcc agaccatgag cgtggcacaa cgtctttatg
     2701 aatcggggca tatcacatac atgcgtaccg attccacaca attgtccaaa atggcacttg
     2761 ccttggcaaa agagatcatc accggaactt tcggagccgg gtacagcaag acaaggcaat
     2821 atgccacccg ttccaaaggg gcacaggaag cccatgaagc catccgcccc actttcatgg
     2881 ataaggttac ggtggaagga actgccacgg ataagaaatt gtacgaattg ataaggaaaa
     2941 gggctcttgc ctcgcaaatg gcagatgcgc aaatagacaa gacccagatc accatcagtt
     3001 gcggaaacac gggcaagcaa ttcatggcta ccggagaggt ggtggttttt gaaggcttcc
     3061 tgaaaatgta ccgggaaggt tccgacgagg atcccgagaa aaacaacgga aaacccacgc
     3121 tggccctgcc tttcctggaa acaggagaca acctgaacgc aagtcagatc agggccattg
     3181 agcgtttcac gcaatccccc taccgataca ccgaggccag cctggtcaag aaactggagg
     3241 agctggggat aggacgaccc tcgacatacg cccccacgat ctccaccatc atagagagac
     3301 aatatgtggt caagggtgac cgtccggcta aaaaaagaaa ctacctggag ctccggctgg
     3361 aaggaaacca ggtaaccagg gaggaatgct ccgagaacta tggagaagaa cgcaagaaac
     3421 tctttcctga agacatcggt atcctggtca atgactttct gatggagcat tttcacgaca
     3481 tagtggatta taattttacc gccaaggtag aagaggattt tgaccggatc gcatcgggaa
     3541 aacttgtctg gaacaaaatg ctggataatt tctacaaacc gttccgcaag accctggaca
     3601 aggcactgga aacctcacac ccgggaaaaa gcgaaagatt gctggggaac gatccggtca
     3661 cgggaaaacc cgtgacagtc aggttgggaa ggtatggtgc gatggcccag ctgggagctt
     3721 ccgatgaccc cgataaacgt tatgcgggac tgcagaaggg ccagctcctg gaatccataa
     3781 cactggaaga ggccctgcgt ctgttcaccc taccccggga ccttggtgtc taccaggatt
     3841 ttcctgtggt cgtatccacg gggcggttcg ggccttacgt caagtggcag ggcaaattca
     3901 tatcactcgc aaaaacggac gatccctata ccgttacgct gcagagaagc atccagctga
     3961 ttgaacaatc gaagaccgaa gattctaaaa aacacatcct ggaattcccg gaagaaggga
     4021 tccggatcct gaagggtcgt tacgggcctt acatcaccca gaataaaaag aattacaaga
     4081 tacccagggg aacagacccc gaaagcctga ccctggagga ttgcatgaaa atcattcaaa
     4141 acaagaacaa tgagtgaaaa acacttcccg ctggaacatc ttcatgcaga tgccctctgc
     4201 agtgagatgc cctcctgtat ggcgatcctt gtctttacag gtaaaggagg ggcagtcctg
     4261 gatgcattgc aatgccttta cccgcaaagc cattcgcgta tttattacat ggaagacacc
     4321 atgcagaccc aactgcccgc catattatcg gctgtagaga aattgcacct tccctgcata
     4381 gtcatgcacc cgggagatat tcccggaatg ggagcgttcc tgccccccga cgcgcgtgta
     4441 agcctggttt cacaaggtac cgaaaacccg gatcacgtca cgggcctgct tgaaaaaaac
     4501 acgccgggtt attttacctg gatcggcttc cagcggtatc tgaccgatcc tgcggtcatg
     4561 gaaaagcttt ccggccactt catggaaacg ctcaggctgg gggaattcag gaaaaaaatt
     4621 acgtccgcag agcccttgct aagggaggcc acccaccatt ttaccgacat gcgatgcgtg
     4681 cgtcatgccg acatgcccga catgaaggaa ggcaatccga acgggctgta tgcagaagag
     4741 atctgcaccc tgggaaggta catgggactt tcacatcatt ttcaactggc agtggtttat
     4801 ggatatccgt ccgattgcag aaaggggtcc ctgaccgcaa aactcaatgc gcagttcata
     4861 tggcatctga cagaaggcct tgctgtatcg gttgaggaac atcccgcaga ggaaagcgtt
     4921 tctttccaga ggaaagccgt gcaaatggga gaagacggcc aggaacttgt gtttttgcaa
     4981 tcccgcaaga gcggaaggtg gtggatggaa gttacaaatt gtaagcagga cggccatccc
     5041 ttttatatct cttgttccat ggacgattac gttatggcat gcaaagggga aatccccctg
     5101 aaatggttat tttactacca gaagtcta
//
LOCUS       DAOSOF010000134         5074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_16629_length_5074_cov_5.496912, whole genome shotgun sequence.
ACCESSION   DAOSOF010000134 DAOSOF010000000
VERSION     DAOSOF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16629_length_5074_cov_5.496912"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..4694
                     /locus_tag="PLM68_07370"
     CDS             435..4694
                     /locus_tag="PLM68_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT55212.1"
                     /translation="MHFILTALTCVAIVTDSLSYKESGAAIEAYRQSIEQGGKQTVLL
                     LDRWHHPDSLRARLYDLYLDQNLEGAILIGDVPIAMVRDAQHLTSAFKMDQDRDWKES
                     SVASDRFYDDFDLRFDYIRADEEKPNYHYYTLRADSPHTISCDIYSSRIVVPEIPGAD
                     RHALLTAYLEKVVRMKAQKEVIDQVLLFTGNGYNSDSMLARLDEAWSLRQQFAGFGTI
                     PGYRFDFINYDFDNVVRPRIKAAMSDPGLDVAILHHHGSEDTQYLGASRINGIQSAFD
                     YLKRFVRSRLGRSKDTTATKEEYKAEYGITDSWFQGAFDPEIIRQDSAYAASMNLSVE
                     DMAGYTPQAKFVMFDACYNGSFYYHDYIGGRYLFQEGNTIVARGNTVNSLQDIWPDEM
                     IGLLQGGVCVGNWAKMNMTLELHLLGDATYAFANTSGTSCLDKEIRQQAENPVFWRQQ
                     LSIATGDFKALALRMLYRNNAISSAELLAIQQSDPSPMVRLEAFTLNKKIADANLKPA
                     VLAALHDDHELLQRMGALTVNLMGDEDLLEPVMEIYFDPTTTVRVNFHLGEVFEQVPY
                     ATFEAAAMAYRANNPLWPTDESFQALLGSLQYAQKSRDEDLAVIAKPDATDKELRWAI
                     PSQRNKQNVLMVDALLTFLRDTSRKPEQRVTTAETLGWYMFSFRKADIVAACREIYAT
                     EQDPAVKNELAKTIGRLTGKAIEVTPMPVRRSFAIVVDNATYQACKAAIEAYRQAVEN
                     DGLTGYVLAGDWQSPEQVKAQLAKYYRHKALEGAVFVGQVPIPMVRRAQHMTSAFKMD
                     QTISWRESSVPSDRFYDDFDLQFDFLKQDSLQPLFFYYNLSGRGPQEITCDIYSGRIK
                     PSLPGEEGYAQIRHYLQKVVAEKAQANKADCLVSYTGEGSFSNSLSAWKDEQITLKEQ
                     FPQAFRTAETAKLYMFYMYPENIKEVLTGELQRKEVDLFLFHEHGVPERQYLTGNPPA
                     THEEAYFADGQRSMRSLMAQQVRFRKFAEGSSEMTDYMRRIEKEYGFDSTWTATYFDP
                     AIREQDSLYDAAQGIMLDDVTNIGPQARMVIFDACYNADFREEDCIATRYILTEGSGT
                     IACFGNSVNVLQDKSSSDLMGLLAAGCRIGEWARNVNILESHIIGDPTFRFAGEDSPD
                     IDFYASDPVYWLDKLQTVPEYDLKGLALYKLHELSYPGMPELLYSTFCESPSYMLRLQ
                     CMHLLAHYDTPLYAQLLKKGSTDPYEFIRRKSIYYMGKVGLDEFIPYVVKTYMDDVQS
                     VRVLHNVSFVAGHFDTGLLRQEFAEAVDKAGYLHDKQAYLDQVNRMIESGESMKQACW
                     KQIVNYLDPGAEKSWYPNSLRNNPYPQLVDALIKALCDTQTNAGFRIQLAEILGWYVR
                     APRRADIVQACHELLADSTTTDPALRDELQKTIYRLSAYMK"
     gene            4696..>5074
                     /locus_tag="PLM68_07375"
     CDS             4696..>5074
                     /locus_tag="PLM68_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT55213.1"
                     /translation="MKKIVLVICFLALGFTAGAVPDKRPASSFAIIIDQASYNACKAE
                     VDAYKAVLDAEGLPAVIVTGDWQTPDQVKAGILKLYKRKPRLEGIVLVGEIPVARVLG
                     GQHFTTAFKMNQNRFPWNECSVPS"
BASE COUNT         1299 a         1265 c         1314 g         1196 t
ORIGIN      
        1 cagcactcaa ttcagctgga gagttaattg tacaaaataa tgaaaaactc acgtaattgt
       61 gaattattta agtcgcattt atgttcaaag ttattgacag aaattgagtg agacgttgca
      121 atatttagta cctttgcaag atattaacca tcggcttcag atgcgcaccc tgcattactc
      181 tatcagaatt agcgacctta tcaaatgcgc aggtatatta aatttatacc gttatataaa
      241 ccagcagggt ttattccaat gcacctggta tcgcttctgc aatttacgcc tctaaaggag
      301 cctgacgggt ttcaaagtcg cttttaggtt cccgcgtgct gatccgggaa accatacatg
      361 ctaagttttt acaatccttt tatctgcctg gcagactaaa gcaaaaaatt tataactatc
      421 aattaattcc ttttatgcac tttatactaa ctgctcttac ctgtgtggct attgtcaccg
      481 attctcttag ttacaaagaa tcgggtgcag ccatagaagc gtaccggcaa tccattgaac
      541 agggaggcaa acagacggtc ctgcttctgg accgctggca ccatcccgat tccctgcgtg
      601 cccggctgta tgatctgtac ctggaccaaa acctggaagg ggccatattg attggcgacg
      661 tgcccatagc catggtgcgc gatgcgcagc acctgacctc ggccttcaag atggaccagg
      721 acagggattg gaaagaatca tcggtggctt cggatcgttt ctatgatgat tttgatttgc
      781 ggttcgacta catcagggcg gatgaggaaa agccgaatta tcattattat accctcaggg
      841 ccgattcccc ccacactatt tcctgcgata tatattcttc acgcattgtg gtcccggaga
      901 tacccggagc cgaccggcat gccctgctga cggcctacct ggaaaaagta gtgcgtatga
      961 aggcacaaaa agaggtcatt gaccaggtgc tcctgtttac cgggaacggg tacaattcgg
     1021 attccatgct ggcccgtttg gacgaagcct ggagtctgcg gcagcaattt gcggggtttg
     1081 gaacgattcc cggttaccgt tttgatttta tcaattacga tttcgataat gtggtcaggc
     1141 cccgcataaa ggcagccatg tctgaccctg gtctggatgt ggccattctg caccaccacg
     1201 gatcggaaga cacccaatac ctgggggctt ccaggattaa cggcattcaa tctgcgtttg
     1261 actacctgaa acgttttgta cgtagcaggt tgggccgttc caaagacaca accgccacca
     1321 aagaggaata caaggctgaa tacggcatta cagacagctg gttccagggt gcgtttgacc
     1381 cggaaatcat ccggcaggat tcagcctatg cggccagcat gaatctgagc gtggaagaca
     1441 tggcgggcta tacaccacag gcaaaattcg taatgtttga cgcctgttac aacggttctt
     1501 tctattacca cgactatatt ggcggacgat acctgttcca ggaaggcaat acgatagtcg
     1561 cacggggcaa taccgttaac agccttcagg atatatggcc cgacgaaatg atagggctgc
     1621 tgcagggagg tgtctgtgtg ggtaactggg ctaaaatgaa tatgaccctg gaactgcatt
     1681 tgcttggcga tgccacctat gcgttcgcca ataccagcgg cacatcctgc ctggataagg
     1741 aaatccggca gcaggcagag aacccggttt tctggcgcca gcagttgtcc attgccacag
     1801 gggactttaa agccctggct ttaagaatgc tgtatcgaaa taatgccatt tcttctgccg
     1861 agttgctggc catacagcag tccgatccca gtccgatggt gcggctggaa gcctttacat
     1921 taaacaagaa aattgccgat gccaacctga aaccagccgt actggccgcc cttcatgatg
     1981 accatgaatt gttgcagcgt atgggagcgc tcacagtcaa cctgatgggc gatgaagacc
     2041 tgctggagcc ggtcatggag atatattttg accctacgac tactgtacgg gtgaacttcc
     2101 acttgggcga agtgtttgaa caggtgcctt atgcaacttt tgaagcagct gcgatggctt
     2161 accgggcaaa taatccgctc tggccaaccg atgaaagctt ccaggcattg ctgggcagcc
     2221 tgcagtatgc acagaaatcg cgcgatgaag acctggccgt catagccaaa cccgatgcca
     2281 ccgataaaga actacgatgg gcaattccct cgcaacgcaa caagcagaac gtgctaatgg
     2341 tagatgcgct gctcaccttc ctgcgcgaca cctcacgcaa accggagcaa cgtgttacaa
     2401 ccgccgaaac gctgggatgg tatatgttct ctttccgtaa agccgatatt gtggccgcct
     2461 gccgggaaat ctatgccaca gaacaggatc cggcggtaaa gaacgaactg gccaaaacca
     2521 ttggccggct gaccggaaaa gcaatagaag tgacgccaat gcccgtccgt agaagctttg
     2581 ccattgtggt agacaatgcc acgtaccagg cctgcaaggc agcgatcgag gcttatcgcc
     2641 aggccgttga aaatgacgga ctcaccgggt atgtactggc aggagactgg cagtcaccgg
     2701 agcaggtgaa ggcgcaactg gcgaaatatt accggcacaa agcgctggaa ggggctgtct
     2761 ttgtggggca ggttcccatt cccatggtgc gaagggcaca acacatgacc tcggctttca
     2821 agatggacca gaccatttca tggcgcgaat catcggtgcc ctctgaccgc ttctatgacg
     2881 attttgactt gcagtttgat ttcctgaagc aggacagcct gcagccgctg ttcttttatt
     2941 acaacctgag cggccgtggc ccgcaggaaa taacctgtga tatttattca gggcgcatca
     3001 agccgtcgct gcccggtgag gaaggatacg cccagatacg tcattacctg caaaaggtag
     3061 tagctgagaa ggcacaggcc aataaggcgg attgtctggt ttcatacacg ggagaaggat
     3121 ctttttcaaa ttcactgtct gcctggaaag atgaacagat caccctgaag gaacagttcc
     3181 cgcaggcttt tcgcacggca gaaaccgcca aactgtacat gttctacatg tatccagaaa
     3241 atattaaaga agtgctgacc ggcgaactgc agcgcaaaga ggtggacctg ttccttttcc
     3301 atgaacacgg ggttcccgag cgccagtacc tcacaggcaa tccgcctgcc acacatgaag
     3361 aagcttactt tgccgatggg cagaggtcaa tgcgctcact gatggcccaa caggtgcgct
     3421 tccgcaagtt tgcggaaggt tcctctgaaa tgaccgacta catgcgcagg atcgaaaagg
     3481 agtacggatt tgattccacc tggacagcca cgtatttcga tccggccata agagagcagg
     3541 attcactgta tgatgctgcg cagggaatta tgctggacga tgttaccaac atcgggccgc
     3601 aggcccggat ggtgatattc gatgcttgtt acaacgccga ttttcgtgaa gaagactgca
     3661 ttgccacccg ctatatcctg acagaaggaa gcggcacgat tgcctgcttc ggcaattcgg
     3721 tgaacgtgct gcaggacaaa tcttcctctg atcttatggg actgctggca gcgggttgcc
     3781 ggataggtga gtgggcaagg aatgtgaaca tccttgaatc ccacatcatt ggagacccga
     3841 ccttccgctt tgccggagaa gacagtccgg atattgattt ttacgcatcg gatccagtct
     3901 attggctgga caaactgcag acggttcccg agtatgacct gaagggactt gcgctctaca
     3961 aactgcatga attgtcttat cccggcatgc ctgaattgtt gtacagtaca ttctgtgaaa
     4021 gtccttccta tatgctgcgc ctgcagtgca tgcacctgct ggcccattac gatacgccac
     4081 tgtatgccca actgctgaaa aagggcagta cggaccctta tgaattcatc cgtcgcaaat
     4141 ccatttacta catgggaaaa gtggggctgg acgaattcat tccctatgta gtcaagactt
     4201 atatggatga cgtacagtct gtccgggtgc ttcataacgt gtcgttcgtg gcgggtcatt
     4261 ttgacaccgg actgttgcga caggaatttg cagaagccgt agacaaggcc ggttacctgc
     4321 acgacaagca agcttatctg gaccaggtga acaggatgat agagagtgga gaaagcatga
     4381 agcaagcctg ctggaaacag attgtaaatt acctggatcc cggggcggaa aagtcttggt
     4441 atcccaattc tttacgtaac aatccctacc cccagcttgt ggatgctctg ataaaggctt
     4501 tatgtgatac gcagaccaat gccgggttcc gtattcagct tgccgaaatc ttaggctggt
     4561 acgtgagggc cccccgccgg gcagacatag tgcaggcatg ccatgaattg ctggcagaca
     4621 gcaccacaac cgatccggca ctgcgggacg aactgcagaa aaccatttac cgtttatccg
     4681 cttatatgaa gtagtatgaa aaaaattgta ttggttattt gtttccttgc ccttggtttt
     4741 actgccggag ctgtaccgga caaacgtccg gctagctcgt tcgccatcat aatagaccag
     4801 gcttcatata acgcatgcaa ggcagaagtt gatgcctaca aggccgttct tgacgcagaa
     4861 gggttacccg ccgtcattgt gacaggggac tggcagacgc cggaccaggt aaaagccggt
     4921 atccttaagc tgtacaaacg caagccacgg ttggaaggca tagtgctggt aggggaaatc
     4981 cccgtggcac gcgtgttggg aggccagcac ttcacgacgg ctttcaagat gaaccagaac
     5041 cgctttccat ggaacgaatg ttccgttcct tcgg
//
LOCUS       DAOSOF010000135         4979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17025_length_4979_cov_3.789805, whole genome shotgun sequence.
ACCESSION   DAOSOF010000135 DAOSOF010000000
VERSION     DAOSOF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17025_length_4979_cov_3.789805"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1364
                     /locus_tag="PLM68_07380"
     CDS             57..1364
                     /locus_tag="PLM68_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT55214.1"
                     /translation="MDIKYTSTEEIKQYQATLLPCTLAYLQEYSPFYKRMFQRDRIDI
                     SEIKTLEDLRYIPFTNKSDLQQYNEDFICVPREKLIDYVTTSGTLSDPVTYAFTNKDL
                     DRLGYNEKVSFECAGAKPGDMVQLMTTMDKRFVAGLAYYLGMRSLGAGVIRVGNGIPE
                     LQWDTINRMKPGIIICVPSFILKLIEYAEENNIDYRKSSIRKAVCIGENLRNQDFSLN
                     ILGRHIREKWDIGLVSTYASTELGATFCECEYGKGGHHLPELVICEVVDDKDRVVLPG
                     ESGELVITNIGLEGMPLLRFRTGDLVRFHYEPCQCGRKTMRISPLLGRKNQMIKFKGT
                     TLYPPSIFDILEDIPFISCYQVVLSSNKLGTDDVNVRISVKEDRCGTPEDVIKAVKDK
                     FRAKVRVAPEVEIVPDRELKSEVYNEKVRKAVKLVDKRNNVPE"
     gene            1351..2493
                     /locus_tag="PLM68_07385"
     CDS             1351..2493
                     /locus_tag="PLM68_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT55215.1"
                     /translation="MSPNNRFDDIRPYTDDEIPAAMQRLASEPILDKVLSWLFPGQPG
                     QDWRNKLLSVKNNEELQERIMHPVAEVIVKRTMKGFTSSGEDKIPGDKGVLYISNHRD
                     IVLDATLFLMILYRKGLPTAEVSFGSNLMQHQAIVDLGKSNKMYKVFRKSGNMRDLVK
                     NSRLLSDYIRYRINGGTSIWIAQHEGRSKDGKDLTDPGLLNMLSMSMKKDRIGALASL
                     NIVPVAVSYEIEPCDVLKVCEIQQTRKQGFYNKTPKEDVVSIVTGITQNKGSVHITVC
                     PAVTINDIEPYRDLPQADFFHQVGKLIDQRIHQGYKLYGINYAACDRLRGTTEFMGQY
                     KEEDMTLLESREKLLPDTDDQTSAALRDLLWRLYANPVLNRMNITL"
     gene            2499..3101
                     /locus_tag="PLM68_07390"
     CDS             2499..3101
                     /locus_tag="PLM68_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOT55216.1"
                     /translation="MAKRIQKIVLLMMLAAVTTGVSVFGQGKQKTAAERTSIGNIIHF
                     FSQPVNYSREPVHPSEIVIFADSLPGVRSEDPAERILDYVWESQREPGRTRFEKLEDG
                     TFLARLIWAPGTDGEEERKDFRNPNKKLRDRPIKDLVYCDGIVYCDGKWIAPYLYHPN
                     FGITANKGVIFFNKDGDLIIRGTKWGFSSTECFKAVDIVP"
     gene            complement(3102..4019)
                     /locus_tag="PLM68_07395"
     CDS             complement(3102..4019)
                     /locus_tag="PLM68_07395"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT55217.1"
                     /translation="MKKNTILLFMIGVLLLPAAVSHAQDQDSSLVPDADSMAFVNGEW
                     ESRLLHFGGITLRQCAFRDSSLFHSNQFVSILVIRNSFRFDILADTALVLTSEFVEYA
                     EALAGINGSFFAWDAPWKSVNYLRVDGNELAPNRIPQDGIRTFRQSGCIAVSDKGRLS
                     IHALPQHVMDTYDQDLWEKELTAEDVLSSGPVLRAAGKDVPVMDNAFNNARHPRTAVG
                     IRRGGRVLMVVVDGRATEAAGMTMQELQRVLSWLRAWDAINLDGGGSSTLCVRKNVRK
                     PVSVVNHPSDNKLFDNQGERRVANVLIVK"
     gene            4057..4737
                     /locus_tag="PLM68_07400"
     CDS             4057..4737
                     /locus_tag="PLM68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOT55218.1"
                     /translation="MERVTGFLSLLSHNNNKEWFDAHKSQYKEALEVFQDFTAELING
                     IATFDKAVSGLSVKDCTFRIYRDLRFSPDKTPYKTYMGAHVCPGGKKSGFAGYYFHIG
                     AAVNDWSGHHFMSSGLYQAHPSVVKSVRDDILYDATPYLNAIALAEGFEIDFGGCLKR
                     VPAGYPSDSPFEHFFRLKDFLLARYMDDAFLFDKNLLKNVLSGFEKTYNFVELLNRSV
                     TYANTSRN"
     gene            4715..>4979
                     /locus_tag="PLM68_07405"
     CDS             4715..>4979
                     /locus_tag="PLM68_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT55219.1"
                     /translation="MPTHPGIKAGIIGFGRMGEMYLLQMLKSTQWQILAICDTDPDAL
                     AVAAHIAPPGTKLTAHEDDIFNDPDITVVVLSTLADSRKRHLFK"
BASE COUNT         1394 a         1185 c         1199 g         1201 t
ORIGIN      
        1 gatacaggag ttcctgctgt ggctggatac agaaattaat cattaagtat aactcaatgg
       61 atatcaaata tacttctaca gaagagatca aacaatatca ggcaaccctg ttgccctgta
      121 cactagcata ccttcaggaa tattcgccct tttacaagag aatgttccaa cgggacagga
      181 ttgatatcag tgagatcaaa acactggaag acctccggta cataccattc accaacaaga
      241 gtgacctcca gcaatacaac gaggatttta tctgtgttcc cagggaaaag ctgatagatt
      301 acgtaactac atcggggaca ctgagtgacc cggtcacgta tgccttcacg aacaaggatc
      361 ttgacaggct cggctataat gaaaaggttt catttgaatg cgcaggggca aaaccgggag
      421 acatggtgca gctgatgacc accatggata agcgctttgt ggcaggcctg gcttattacc
      481 tgggcatgcg aagcctggga gcaggtgtca tacgcgtggg aaacggcatt cccgaactgc
      541 aatgggatac catcaacagg atgaagcccg gtatcataat ctgtgttcct tctttcattc
      601 ttaaactgat cgaatacgcc gaggaaaaca acatcgatta caggaaatcc agcatccgga
      661 aagctgtctg cattggtgag aacctgcgta accaggattt ttcgctcaat atccttggca
      721 ggcatatccg ggaaaagtgg gacatcgggc tcgtatccac ctatgcctcg acagaactgg
      781 gcgccacatt ctgcgagtgt gaatacggga aaggaggaca ccaccttccg gaactggtca
      841 tctgtgaagt ggtagatgac aaggaccgcg ttgtgttgcc gggtgaaagc ggcgaactgg
      901 tgatcacaaa catcggcctg gaaggaatgc ctttgctacg gttcaggacg ggagatcttg
      961 tccggttcca ctatgaacct tgccagtgcg gaaggaaaac aatgagaatc agtcctttgt
     1021 taggtcgaaa gaatcaaatg ataaaattca agggtacaac cctctatcct ccttccatat
     1081 ttgatatatt ggaagacatc cccttcataa gctgttacca ggttgtttta agcagcaata
     1141 aactgggaac ggacgatgtg aatgtcagga tatccgtgaa ggaagacaga tgcggcaccc
     1201 cggaagatgt cattaaagcg gtcaaggaca aattccgggc aaaggtacgt gttgcccccg
     1261 aggtggaaat agttccggac agggaactca agtccgaggt ctataacgaa aaagtgcgca
     1321 aagccgtaaa attggtagat aaaaggaata atgtccccga ataatagatt tgacgacatt
     1381 cgtccctata ccgatgacga gatcccggca gccatgcaac gtcttgcttc agaacccata
     1441 ctggataagg ttctttcgtg gctgttcccg ggacagcccg gccaggattg gcggaataag
     1501 ctgctctcgg tcaagaacaa cgaggagctc caggaacgta tcatgcatcc tgtggcagag
     1561 gttatcgtca aaaggacgat gaagggattt accagttcgg gagaagataa gattcccggc
     1621 gataaaggtg ttctttacat atcaaaccac agggacatag ttctggacgc gaccctgttt
     1681 cttatgatcc tgtaccggaa aggactcccc acagcagaag tatctttcgg tagcaacctg
     1741 atgcagcatc aggcgatcgt ggacctgggg aaatctaaca agatgtacaa ggttttccgt
     1801 aagtcgggga atatgcgtga tctggttaaa aattccaggc ttttatccga ctatatccgt
     1861 tacaggatca acgggggcac ctcaatctgg attgcacagc acgagggacg gagtaaagac
     1921 ggtaaggacc ttaccgatcc cggtctgctt aacatgcttt ccatgagtat gaaaaaagac
     1981 cgtatcggcg cacttgcctc actaaatatt gttccggttg ctgtttctta tgagattgaa
     2041 ccttgtgatg tcttgaaggt ctgcgaaata caacagacca gaaaacaggg tttctataat
     2101 aaaacaccca aagaagacgt tgtcagtatc gtgacaggga tcacgcaaaa caaagggagt
     2161 gtacatatca cagtctgccc tgcagttacc atcaacgaca ttgaacctta cagagacctt
     2221 ccacaggccg acttttttca ccaggtggga aaactgatcg atcagcggat tcaccaggga
     2281 tacaaactat atggtataaa ttatgcagct tgtgacaggc ttagaggaac aaccgaattt
     2341 atggggcaat ataaggaaga agacatgaca cttcttgaaa gccgtgaaaa gctgctccct
     2401 gacactgatg atcaaaccag cgctgctcta agagaccttc tatggcgact gtatgccaat
     2461 cctgttctaa accgcatgaa tatcacctta tgagtgtcat ggcaaaaaga atacagaaaa
     2521 ttgtcctgct gatgatgctg gccgctgtca cgacgggtgt ttctgttttt ggccagggga
     2581 agcagaaaac ggcagcagaa cgcacctcga tcggaaacat cattcatttc ttttctcaac
     2641 cggtgaatta ctcccgtgag ccggttcacc cctcagagat agtgattttc gcagactctc
     2701 tgccgggcgt cagatctgaa gatccggcag aaaggatcct ggactatgta tgggagtcac
     2761 agcgtgaacc aggcagaacc cgttttgaga aactagaaga cggaacgttc ctggcccgcc
     2821 tgatatgggc tccgggtaca gacggagaag aagagcgcaa agatttcagg aatcctaata
     2881 aaaaactcag ggacaggcca ataaaagacc ttgtatactg tgacggcata gtgtactgcg
     2941 atggtaaatg gatagcacct tacctgtacc accccaactt tgggatcact gccaacaagg
     3001 gagttatttt tttcaacaaa gacggcgatt tgatcatccg tggaaccaaa tggggttttt
     3061 cctccactga atgcttcaag gcagttgaca tagtgccttg atcatttgac tatcaaaaca
     3121 ttggccacac gcctttctcc ctggttgtca aagagtttat tgtccgatgg gtggttgacc
     3181 accgatacag gtttccgtac attcttgcgt acacataatg tagaagaacc gcccccgtcc
     3241 aggttgatgg catcccaggc cctgagccag ctcaggacac gttgcagttc ttgcatggtc
     3301 atgccggcag cttctgttgc ccttccgtcc actaccacca taagaacccg gcctcccctt
     3361 cttattccca cggcagtcct gggatgacgt gcattgttga aagcattgtc catgaccggc
     3421 acatccttcc ctgctgcacg taaaacaggc cccgaggata gtacatcttc tgcagtcagt
     3481 tctttttccc acaggtcctg gtcatacgta tccataacat gttgcggaag cgcatgaatg
     3541 gacagtctgc ccttgtcaga tactgcaatg cagccggatt gccggaatgt ccgtattcca
     3601 tcttgcggga tcctgttggg cgccagttca ttgccatcaa cgcgaaggta gttgactgac
     3661 ttccatggag catcccaggc aaaaaaggag ccgttgatcc cggccagggc ctctgcatat
     3721 tccacaaatt cactggtaag cacgagcgca gtatcggcca ggatatcaaa acggaaagag
     3781 ttcctgatta ccagaataga tacaaattga ttggaatgga aaagggaact gtccctgaaa
     3841 gcacattgac gaagggtgat accgccaaag tgtaacagcc tgctttccca ttcgccattc
     3901 acaaaagcca tggagtccgc atccggaacc agggaagagt cctggtcctg tgcgtgtgat
     3961 acagcggcag gcagcagcaa tactcctatc atgaatagta atatggtatt ctttttcatg
     4021 cttcaaatct ttatatttgc taaaattaca aaaattatgg aacgggtaac cggttttctt
     4081 tccttactga gccataacaa caacaaagaa tggtttgatg ctcacaaatc acaatacaaa
     4141 gaagctctgg aagttttcca ggactttacc gcagagctga tcaatggcat tgccacattt
     4201 gacaaggctg tgagcggcct tagcgttaag gattgcacat tcaggatata ccgggacctg
     4261 aggttttccc ccgataaaac cccctacaag acctatatgg gagcccatgt atgcccgggt
     4321 ggtaaaaaat ccggattcgc cggttactat tttcatatag gggctgccgt aaacgactgg
     4381 tccggacacc atttcatgag cagcggcctg tatcaggccc atccttctgt ggtgaaaagt
     4441 gttcgggatg acatactcta cgatgccacc ccctacctga atgccatagc cctggcagaa
     4501 ggattcgaaa ttgactttgg cggatgcctg aagcgtgtcc cggcaggata tccctccgat
     4561 tcaccttttg aacatttctt caggcttaag gatttccttt tggcacgata tatggacgat
     4621 gcattccttt ttgacaagaa tctgttaaaa aacgtcttgt ccggatttga aaagacctat
     4681 aactttgttg aattgttgaa ccgatccgtt acttatgcca acacatcccg gaattaaagc
     4741 aggtatcata ggcttcggcc ggatggggga aatgtaccta ctccagatgc tcaaaagcac
     4801 ccagtggcag atcctggcca tttgcgatac ggaccccgat gcgcttgccg tagctgcaca
     4861 tattgccccg ccaggtacaa aacttactgc ccatgaagat gacattttca atgatcctga
     4921 tataaccgtt gtggttttgt ccacccttgc cgattcgcgc aaaaggcatc ttttcaagg
//
LOCUS       DAOSOF010000136         4959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17101_length_4959_cov_5.935767, whole genome shotgun sequence.
ACCESSION   DAOSOF010000136 DAOSOF010000000
VERSION     DAOSOF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17101_length_4959_cov_5.935767"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLM68_07410"
     CDS             complement(<1..288)
                     /locus_tag="PLM68_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOT55220.1"
                     /translation="MHLFYSPHIQIPEHTLPPDESLHCTRVLRLKKSDTVHLTDGKGT
                     LCTGKVLDPDPAGCRIQITHITENHGARPYHLHLAVAPTKNMERFEWFVEKA"
     gene            complement(294..689)
                     /locus_tag="PLM68_07415"
     CDS             complement(294..689)
                     /locus_tag="PLM68_07415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT55221.1"
                     /translation="MKPEKVQRTPIQIRFNDIDMLQHVNNAMYVHYCDLARGDYFRHQ
                     TGYYPYFVNDVKLLIVVHTEFDFLVPSLMTDSIFVETRLEKVGERSLHFLHEIVDDKG
                     NLRVRSRCVMSTFDKSTGKSFPLSDVWKF"
     gene            complement(686..1267)
                     /locus_tag="PLM68_07420"
     CDS             complement(686..1267)
                     /locus_tag="PLM68_07420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT55222.1"
                     /translation="MHNDQHIIDLTDTAVQVLRSGGTILYPTDTVWGLGCDACNPQAV
                     KKVSGIKGRTADKHYIILVADLNMLSRYVEQIPHMAESLLEVADKPLTIIYPGGINLA
                     PGVTAPDGSIAIRIPVHDFCHALIRKLKRPLLSTSANLSGIKTPSRYADIDRELLKKV
                     DWTAPAFLEAGSTGQPSSIIRLGINNQIKIIRP"
     gene            complement(1297..1983)
                     /locus_tag="PLM68_07425"
     CDS             complement(1297..1983)
                     /locus_tag="PLM68_07425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT55223.1"
                     /translation="MNQYNRGGFFASIPFVIRNIIGINALMLILTMINREFMLENFAL
                     FFPSSQFFKPWQVITHIFMHGNFGHLLFNMYALWMFGSILEQLWGSKKFMLYYLVTGL
                     GAAALHMGVQWIEASSLVREINENGMEAMEALTRYRMLMSTPTVGASGAVYGILLAYG
                     MLFPNNELRIIFLPIALKAKYFVIIFAVIELILGITGGGNIAHFAHLGGMIFGFILVR
                     YWKKKNRLYY"
     gene            complement(1988..3763)
                     /gene="mutL"
                     /locus_tag="PLM68_07430"
     CDS             complement(1988..3763)
                     /gene="mutL"
                     /locus_tag="PLM68_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT55224.1"
                     /translation="MIHVLPPHIASQIAAGEVIQRPASVIKELMENAVDSGADRIDVV
                     LTDGGATLMQVTDNGCGMGPEDALLAFERHATSKIRGVEDLQKVQTMGFRGEALASIA
                     SIAHVRLRTCRKEDEMGTEVTIAGSAVTSQEEVVCPQGSDFCVRNLFYNVPARRRFLK
                     SEPVEFRHILEEFHRVVLCHPEITFTLTHNGSPVYHLPVSDNLRARITGTLGKEWNAQ
                     LLDISVDTSIVQIRGYVGRPRDSYKKGGSRYFFVNGRFFKSPFLHKAVMNAYGKLLPQ
                     DKLPSYFIYLETEPAFIDVNIHPTKTEIKFEDESVVFQMLQAVIRETLGKFALGPSID
                     FEIGAMQHHIPIPPAPGEHVAPPNLKYDPDFNPFRETGYETVRPPRQQYDALFKAFGE
                     TGQETDPAMFAQEQAGSYEAPVIPLRKKYLATPVASGLLVIHKQRAMERIFYEQILPR
                     FKDRPPVPERLYFPVALNLQPSEAMLLEKSRDLLLRMGFETDENLAITSVPPEHPTDE
                     GSIMDSVREILSVQGEEPLETPYGERLAALLSRRMAAAEQNNHDPANHFLAERLFACR
                     EPHSTPDGRACTTLITFEQIDKIFI"
     gene            complement(3760..4092)
                     /locus_tag="PLM68_07435"
     CDS             complement(3760..4092)
                     /locus_tag="PLM68_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55225.1"
                     /translation="MAFRISFFRTPQHRVFNYKPVYWDPKKEEFEERVKQAREKYSVK
                     KTSEGDAPYVPGSLIRGSFRKRPELKRGYPARQKMIRLVVILLLAALLVAIFYLSNAF
                     GYLFSVFQ"
     gene            complement(4104..>4959)
                     /locus_tag="PLM68_07440"
     CDS             complement(4104..>4959)
                     /locus_tag="PLM68_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012967389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT55226.1"
                     /translation="ILAPVKLLGKSKEEATAQARELLQIVGLLAKEKAYPSELSGGQK
                     QRVAIARCLAMKPDIILFDEPTSALDPTMVSEVLGVIRRLAGTGMTMVIVTHEMQFAR
                     DVSTRVLYMDQGIIYEEGPPSQVFDNPQKEATRTFINRIRNLEWLTEDYEHFDIYALT
                     TEVEQFCEKHFLGPARKNDIMHLVEETMQLCLGTDNQPETLSRRELIRQTGGILLFVS
                     YAEKTNQINVRFSADARLETILNSARDEDGLGMMIINGLTAGQVNERTAGDRIILDLL
                     LATRPANN"
BASE COUNT         1061 a         1242 c         1421 g         1235 t
ORIGIN      
        1 ggccttttcc acgaaccact cgaatcgctc catgttcttg gtgggggcaa cggccaggtg
       61 caggtgatag gggcgcgcgc cgtggttctc tgtgatatgt gtgatctgta tgcggcagcc
      121 ggcggggtcg gggtccagca cctttcctgt gcacagggtg ccttttccgt ctgtcaggtg
      181 aacagtgtct gattttttca gccgcaacac ccgggtgcaa tggaggcttt catcgggtgg
      241 gagcgtgtgc tccgggattt ggatatgcgg tgaatagaaa agatgcatga tcgtcagaac
      301 ttccagacat ctgacaaggg aaaggatttt ccggtggatt tgtcgaacgt ggacatcacg
      361 cagcgggagc gtacccgcag gttgcccttg tcatccacga tttcatgcag gaagtggagg
      421 ctccgttccc ccactttttc caggcgggtt tccacaaaaa tggaatcggt catcaggctg
      481 gggaccagga aatcaaactc ggtatggaca acaatgagca atttgacgtc attgacaaaa
      541 tagggataat acccggtctg atgcctgaaa taatctcccc gcgccaggtc gcaatagtgc
      601 acgtacatag cattgttcac atgctgcagc atgtctatgt cattgaaacg gatctggatg
      661 ggggttcttt gtactttctc tggtttcatg gtctgatgat ctttatctgg ttgttgattc
      721 caaggcgtat gatggaggag ggttgtcccg tggagcctgc ttccaggaac gcgggagccg
      781 tccagtctac ctttttcagg agttcccggt caatatcggc ataccgggag ggggttttta
      841 taccgcttag gttagccgag gtggagagca gaggccgttt cagcttccgg atcagggcgt
      901 ggcagaaatc atggacgggg atgcgaatgg caatgctgcc gtccggggct gtcaccccgg
      961 gggccaggtt gatgccgccc ggatatatga tggtcagggg cttgtctgcc acttccagca
     1021 gggattcggc catgtggggg atctgctcca cgtagcgtga aagcatgttc aggtctgcca
     1081 ccaggatgat ataatgctta tcggccgtgc gccctttgat cccggaaacc tttttcaccg
     1141 cctgcggatt gcacgcatcg cagcctaaac cccataccgt atcggtaggg tacaggatgg
     1201 ttccgcccga gcggaggacc tggaccgccg tgtctgtaag atctatgata tgctggtcat
     1261 tatgcatggt tttcgcggtc tttgctcatg agtgaatcaa taatagaggc ggttcttttt
     1321 cttccagtaa cgcaccagta tgaatccgaa gatcatcccg cccaggtggg caaagtgggc
     1381 gatgttgcct cctccggtaa tgcccagaat cagttcaatc acagcgaata tgatcacaaa
     1441 atacttggcc ttcagcgcaa taggtaagaa tatgatgcgc agttcattgt tggggaaaag
     1501 cattccatac gcaagcagga tgccgtatac ggcccctgac gcgcctaccg tgggggtgct
     1561 catgagcatg cggtaccggg tcagtgcttc catggcttcc atgccgttct cgttgatttc
     1621 ccttaccagt gaagaggcct ctatccactg cacacccatg tgaagggcag cagcacccag
     1681 tcctgtgacc aggtaataga gcatgaattt tttagagccc cagagctgtt ccaggatgga
     1741 tccgaacatc cacagtgcat acatgttgaa gagcaggtgt ccgaagttcc cgtgcatgaa
     1801 gatatgtgtt atgacctgcc agggcttgaa aaattgtgag gaggggaaga aaagggcaaa
     1861 attttccagc atgaattccc ggttgatcat cgttaaaatg agcattaaag cattgatccc
     1921 gatgatgttc cggataacga acggtatgct ggcgaaaaat ccgccccggt tgtattgatt
     1981 catagtatca gatgaaaatt ttatctattt gttcaaaagt gatgagggtg gtgcaagccc
     2041 ttccgtcggg cgtgctatgg ggctcccggc aggcgaacag gcgttctgcc aggaagtggt
     2101 tcgcaggatc gtgattgttc tgctccgcag cagccatcct gcgggagagc agagctgcca
     2161 ggcgttctcc gtaaggggtt tccaggggtt cttctccctg gacggaaagt atctcacgga
     2221 ccgagtccat tatagagccc tcatcggtgg gatgttccgg gggaacggat gttatggcca
     2281 ggttttcgtc tgtttcaaaa cccatgcgaa gcagcaggtc ccggcttttt tccagtagca
     2341 tggcctcgga aggctgcaag tttagtgcca cagggaaata cagccgttcg ggcactggtg
     2401 ggcggtcttt gaatcggggt aggatctgct cgtaaaaaat ccgttccatg gccctttgtt
     2461 tatggatgac cagcaacccc gaggccaccg gggtggccag gtatttcttg cgcaggggga
     2521 tgaccggtgc ttcataggac ccggcctgtt cctgtgcaaa catagccgga tcggtttcct
     2581 ggccggtttc cccgaaagct ttgaaaagcg catcgtattg ctggcgtggc gggcgcaccg
     2641 tctcataacc ggtttcacgg aaaggattga aatccggatc gtatttcagg ttgggcgggg
     2701 caacatgttc gcccggggcg ggtggaatgg gaatgtggtg ctgcatggcc cctatctcga
     2761 aatctatgct cggtcccagg gcaaacttgc ccagggtttc acggatgaca gcctggagca
     2821 tctggaagac tacgctttca tcttcaaact tgatttcggt cttggtggga tgtatgttga
     2881 cgtcaatgaa agcgggttcg gtttccaggt agatgaaata agaggggagc ttgtcctggg
     2941 gcagcagttt tccgtaggcg ttcatgactg ccttgtgcag gaacgggctt ttgaagaacc
     3001 tcccgttcac gaaaaaataa cgggaccctc cttttttgta ggaatcgcgc gggcggccca
     3061 catagcccct gatctgcact atggaggtgt ccacactgat atccagcaat tgggcgttcc
     3121 attctttacc cagggtaccg gtaatgcggg cgcgcaggtt gtccgacaca ggcaggtgat
     3181 agaccgggct tccgttgtgg gtcaaagtaa aagtaatttc agggtggcaa agcacaacgc
     3241 ggtgaaattc ctccaggatg tgcctgaatt ccacaggttc ggacttcagg aagcgccggc
     3301 gcgccggaac gttgtaaaaa aggtttctta cacaaaagtc cgatccctgg ggacaaacaa
     3361 cttcttcctg cgatgtgacg gcagacccgg caatggtcac ttcggtcccc atctcgtcct
     3421 ctttcctgca ggtgcgcaac cgcacgtggg ctatggaagc gatggaggcc aaagcctcgc
     3481 cccggaatcc catggtttgc actttttgca ggtcttccac cccccggatc ttggatgtgg
     3541 catggcgttc gaaagccagc agggcgtctt ccggtcccat gccgcaaccg ttgtcggtta
     3601 cctgcatgag cgtggccccg ccatcggtca gcaccacatc aatccggtct gcccccgaat
     3661 ccacggcgtt ctccatcaat tccttgatga cagaggccgg ccgctggatg acttcacccg
     3721 cagcgatctg actggctata tggggaggaa gaacatggat cattggaaaa cgctgaataa
     3781 ataaccaaag gcattggaca ggtagaagat cgctacaagg agcgcggcca gcaacaggat
     3841 aacaactaac ctgatcattt tctgccgtgc gggatacccg cgtttcaatt caggccgttt
     3901 ccggaaagaa ccacgtatga gcgaaccggg aacatacggg gcgtcgcctt cgcttgtttt
     3961 cttgacggag tacttttcac gggcctgttt cacccgttct tcgaattctt cttttttggg
     4021 gtcccagtaa acgggtttgt agttgaaaac ccggtgctgg ggagtgcgga aaaatgatat
     4081 tctgaatgcc atagccttac tttttaattg tttgccggcc ttgttgccag gagcaggtcc
     4141 agtatgatcc ggtctcctgc ggtccgttcg ttgacctgtc ctgccgtaag gccgttgatg
     4201 atcatcatgc ccaggccgtc ttcgtcccgg gcactgttaa gaattgtttc cagccgggca
     4261 tcggcagaaa aacgcacgtt tatctgattg gttttctctg cataggaaac aaacagcaat
     4321 atgccacctg tctgacggat gagttccctg cggcttaggg tttccggctg gttgtccgtt
     4381 cccaggcaca attgcatggt ttcttccacc aggtgcatga tatcgttttt tctggcgggg
     4441 ccaagaaaat gtttttcaca gaactgttcc acttctgttg tgagggcgta aatgtcgaag
     4501 tgttcataat cttccgtaag ccattccagg ttgcggatgc ggtttatgaa agtccgcgtg
     4561 gcctcttttt gcgggttgtc gaaaacctgt gaaggaggcc cttcttcata aatgattccc
     4621 tggtccatat acagaacccg ggtggacaca tcgcgggcaa attgcatttc gtgcgttacg
     4681 attaccatgg tcatgcctgt tccggccagg cggcggatca cacccagcac ttcgctgacc
     4741 atggttgggt ccagggccga ggtgggttcg tcaaacagga tgatatccgg tttcatggcc
     4801 aggcagcgcg caatggccac cctttgtttc tgacccccgg aaagctcaga gggatatgct
     4861 ttctctttgg caagcagtcc cacaatctgg agcagttcac gggcctgggc ggtggcttct
     4921 tccttgcttt tgcccagcag tttgaccggc gccaggatc
//
LOCUS       DAOSOF010000137         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17200_length_4938_cov_4.839648, whole genome shotgun sequence.
ACCESSION   DAOSOF010000137 DAOSOF010000000
VERSION     DAOSOF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17200_length_4938_cov_4.839648"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLM68_07445"
     CDS             complement(<1..379)
                     /locus_tag="PLM68_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55227.1"
                     /translation="MKGIYFWTLAVIITLAAVFLQRNTGPSHPGKEVMEVNGSFFKAS
                     FPRSLIRPRDNASNTKLTIELSSTDNAQDRIFGAILYYRQYPGSGNYSAIVPVFATAK
                     DKLLVNCMIPVQPTAGKISYYLQL"
     gene            complement(376..1608)
                     /locus_tag="PLM68_07450"
     CDS             complement(376..1608)
                     /locus_tag="PLM68_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT55228.1"
                     /translation="MKSASLVIGTVVFTATVFFLCNFRKPRHTPDLRSLREVVEVLSS
                     PDFEGREAGSKHDSAIASYIANRMASYGFEPYFPEGPLHRFRYRNVESWNVVMVYRGK
                     NTSGALLVGAHYDHLGTGGKASGSLRPDTMAFHPGADDNASGVSAAMEAARLLKESTT
                     RKRLKKDIIFAAFGAEEKGTIGSAMLADTLEKMGSLPSLMINLDMVGRLRDSVLQLGG
                     TGTFQSADSLIDAQLDPTLPLVLKKSESGYGPSDHHVFYRSQVPVLFFSTPPHQDYHT
                     PFDTPDKINYPGMAAIVTCVTDVARAVVVDGFEPLYRETIDTGSLPEQVRFKVSLGVI
                     PDFTYEGEGFCAGTVIKGRPSHKAGMEDGDVIIQVNDIPVKDIESYMEILGRLETGQL
                     VRITVRRNGKNIELEVQL"
     gene            complement(1613..2662)
                     /locus_tag="PLM68_07455"
     CDS             complement(1613..2662)
                     /locus_tag="PLM68_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT55229.1"
                     /translation="MKKSIYTLVSLLGIFLLGSCNINTYNITATFPDDSFNGQVAMLT
                     ASSDGSVLDSVVIAENKAFFTGQVKEPALCMIKSDRTRGQLILEKGDIAIEMKLPGEV
                     SVGTGTPLNDALGGLITRYNELMDEIKNYQGEDEEGYFDSVWTPKFTQEIGDIFKANN
                     NNDIGTMALMYLSNYGEKDSIDSFFAQAGKVVTSRGPIQKIIKQREALKLTAEGTMFS
                     DFTIEDSAGQSVAFSEYIGKGKYVLVDFWASWCGPCKAELPNIREIYDKYNGEDFTVL
                     GVAVWEKPEDTKKGIEELGIIWPCIINAQSIPTDIYGINGIPHIILFGPDGTIVARGL
                     RGEAMKAKVAEVLGR"
     gene            complement(2707..3645)
                     /locus_tag="PLM68_07460"
     CDS             complement(2707..3645)
                     /locus_tag="PLM68_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOT55230.1"
                     /translation="MKILSVNLSEAKGTVKKAMESVTLDKSGVCGDAHAGPWHRQVSL
                     LGEEAYRMMELDPEAFPYGSFAENITTEGAPGPDIPDLKSCRPGDRFNCGDVLLEVTQ
                     IGKECHGPGCAIYEKSGRCVMPKEGIFTRVLSGGILRPGDNLTYDPKKYHIAVITLST
                     RAYNGFYTDRSGPVLIELIADFCSQNDWPCEISGVLLPDDAAKLHNELRKFIRAGYDM
                     VFTSGGTGIGPSDITIGVVRRLLQREIPGIMEYIRISCGKDNPHALLSASLAGVSENT
                     LVFALPGSVNAVKEYFSVISRSLKHMVYMRMELDVH"
     gene            complement(3626..4108)
                     /gene="moaC"
                     /locus_tag="PLM68_07465"
     CDS             complement(3626..4108)
                     /gene="moaC"
                     /locus_tag="PLM68_07465"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOT55231.1"
                     /translation="MKQELSHTRKDGRVHMVDTSSKENSLREAKACGSIALSSDTIQL
                     IKEDQVKKGSVLTTAALAGISAAKQCSNLIPLCHNIPLAKADVTFTMHPDRIEAFSEV
                     RCVGPTGVEMEALTAVSIALLTIYDMCKAVDKSMEIKEIKLLKKTKTSLLYCDENPVC
                     "
     gene            complement(4123..>4938)
                     /locus_tag="PLM68_07470"
     CDS             complement(4123..>4938)
                     /locus_tag="PLM68_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT55232.1"
                     /translation="PMTDSFGRQINYLRISVTDRCNLRCIYCMPAEGVPPVAHEQLLS
                     YEEILAFTRYAVSRGISKVRLTGGEPLVRKGITGLVRSLAGMEGIKDLAMTTNGILLT
                     AMAVELKEAGLHRLNISLDTLDPEKYRQLTRGGDVNKVLQGIRTAKKAGFGGAGSPLK
                     LNCVLLPETGEDELARLRDFAREEGLKLRLIQYMDLKNGSFTRVDGGDGGDCPRCNRL
                     RLTSTGMLKPCLFSNTGYDIRKLGAAKALDTALLEKPGAGTFNSCETFNYLGG"
BASE COUNT         1139 a         1286 c         1186 g         1327 t
ORIGIN      
        1 ggagctgcag gtaataactt attttcccgg cagttggctg taccggtatc atgcagttaa
       61 ccagcagttt gtccttagca gtcgcaaaaa caggaactat ggccgagtaa tttccgctgc
      121 ccgggtactg cctataatag agtatggccc caaagatcct gtcctgtgca ttatcggttg
      181 aacttagttc tatggttagt ttcgtattgg atgcattgtc acgcgggcgt atcaggcttc
      241 tcggaaagga agctttgaaa aaagaaccgt tgacttccat cacttcctta ccgggatggc
      301 tcggccccgt atttctttgt aaaaaaacag cggccagtgt gattatcacc gctaaagtcc
      361 agaaatatat tcctttcata attgtacctc cagttctatg ttttttccgt tcctgcggac
      421 agtaatacgg acaagttgtc ccgtttcaag ccggccgagg atttccatat aggattcgat
      481 gtctttaacg ggaatgtcat tgacttgtat gatcacatct ccatcctcca tgccggcctt
      541 gtgggaaggt cttcccttga taaccgtacc ggcacagaat ccttctcctt catacgtgaa
      601 gtcggggatt acccccaggc tgactttaaa cctgacctgt tccgggagtg agccggtatc
      661 tatggtctcc ctgtacagag gttcaaagcc gtccacaaca acagcccgtg ccacgtcagt
      721 aacacaggtg acaatggcag ccattcccgg gtaattgatc ttatcgggtg tatcaaatgg
      781 cgtatggtaa tcctgatgcg gcggggtgct gaagaaaagc accggtacct gtgaacggta
      841 aaaaacgtgg tgatcggaag gaccatagcc gctttccgat tttttcagga ccaggggcag
      901 cgttggatcc agctgtgcgt caatgagtga atctgccgac tggaaagttc ccgtcccgcc
      961 caactgcaac acgctgtccc gaagccttcc caccatatcc agattgatca tcagcgaggg
     1021 gagggacccc attttttcaa gcgtgtcagc cagcattgcc gagccgatgg tacctttttc
     1081 ctcggcaccg aaggcagcaa atatgatgtc tttttttaat ctcttccttg tggtgctttc
     1141 cttgagcagc ctggctgcct ccatggctgc agacactccg gatgcattgt cgtcagcacc
     1201 cggatggaaa gccatggtat ccggccttaa gcttcccgag gcctttccgc cagttcccag
     1261 atgatcgtaa tgtgcgccca ccagcagggc tcctgatgtg tttttgccgc gatacaccat
     1321 taccacgttc caggattcca cattgcggta acggaaacgg tgcaggggcc cttccggaaa
     1381 gtacggctca aatccatagg aggccatacg atttgcaata tatgacgcta tggctgaatc
     1441 atgcttgctt cctgcttcac gtccttcaaa gtccggagaa gagagtactt ccacgacttc
     1501 tctaagagag cgtaaatcag gcgtgtgacg cggtttccta aaattacaaa gaaagaacac
     1561 ggtggctgta aagaccaccg tgccaatcac cagggaagca gatttcatgt tgttatctgc
     1621 ccaatacttc agctacttta gctttcatgg cttctccacg caggccacgg gcaactatgg
     1681 tgccgtccgg cccgaagagg atgatgtgag gtataccatt aatgccatag atgtcggtag
     1741 gaatgctttg ggcattgatg atgcagggcc agataatgcc cagttcttca atgccttttt
     1801 tggtatcttc cggtttttcc catacagcaa ctcccagaac ggtgaaatct tccccgttat
     1861 acttatcgta aatctccctg atgttaggga gctcagcttt gcagggtccg caccagctgg
     1921 cccagaaatc taccaggacg tacttccctt tcccaatata ttccgagaaa gccacgcttt
     1981 gtccagccga atcttcaatg gtaaaatctg agaacattgt tccttcggca gtcagtttta
     2041 gggcttcacg ctgtttgatg atcttctgga tgggtcccct ggaggtcaca acttttcctg
     2101 cctgtgcaaa aaaggagtca atagagtctt tttcgccata gttggagagg tacatcaggg
     2161 ccattgtgcc aatgtcattg ttgttattgg ctttgaaaat atcaccaatt tcctgggtga
     2221 atttcggagt ccagacgcta tcgaagtatc cttcttcatc ttctccctga taatttttga
     2281 tctcgtccat aagctcgtta tacctggtga tcagacctcc aagggcatca ttaagaggag
     2341 ttccggtacc cactgaaacc tcaccgggaa gtttcatttc aattgcgata tcgccttttt
     2401 ccaggatcag ctgaccacgg gtacgatcac tttttatcat gcacagtgcc ggttctttaa
     2461 cctggccggt aaagaaggct ttgttttctg ctatgaccac actgtccaga acgctgccgt
     2521 cgctggaagc tgtgagcatg gctacctgcc cgttgaagga atcatcagga aaggttgctg
     2581 taatattata tgtattgata ttgcaggaac ccagcaggaa aatccctaaa agggaaacca
     2641 aggtgtaaat ggattttttc ataaacaaac agtattaaat tatttcgcaa agataatgga
     2701 aaaacattaa tgaacatcta gttccatgcg catgtaaacc atatgtttca aactgcgtga
     2761 tatgacggaa aaatactctt tcacggcatt cacgctgccg ggcagtgcaa agacaagtgt
     2821 attttccgaa acacccgcca gcgaagcact cagcagggca tgcggattgt ctttgccaca
     2881 ggaaatcctt atgtattcca tgatgccagg gatctccctc tgaaggagcc gtctgacaac
     2941 ccctatggtt atatccgagg gccctatccc cgtacccccg ctagtaaaga ccatatcgta
     3001 ccctgcacgg atgaatttcc tgagctcgtt atgcagtttt gccgcgtcat cgggcaacaa
     3061 tacaccggaa atttcacaag gccagtcgtt ttgactgcag aaatccgcta tgagttcaat
     3121 cagtaccgga ccgctacggt ctgtgtaaaa gccgttataa gccctggtgc tgagcgtgat
     3181 gaccgcaata tggtattttt tgggatcgta tgtaaggtta tcacccggtc tgagaatgcc
     3241 ccccgaaagc acacgagtga aaattccctc ctttggcatg acgcaccggc cgcttttttc
     3301 atagatcgca caaccgggcc catggcattc ttttcctatc tgtgtgacct ccagcaaaac
     3361 atctccgcaa ttgaacctgt ccccggggcg gcaggatttc agatccggga tatcgggccc
     3421 gggggctccc tctgtggtta tgttctcggc aaacgaccca tagggaaatg cttcaggatc
     3481 cagttccatc atccggtagg cctcttctcc aagcaggctt acctgcctgt gccatggacc
     3541 ggcatgggca tccccgcaca cacccgattt gtcaagcgta acggactcca tagctttttt
     3601 tactgttcct tttgcttcgg aaagattaac agacaggatt ttcatcacaa tacagtaagc
     3661 ttgtttttgt tttcttaagt aattttatct ccttgatctc catgctcttg tcgactgcct
     3721 tgcacatgtc gtatatggta aggagcgcta tgcttacggc cgttaaagct tccatttcaa
     3781 ccccggtggg cccgacgcag cggacttcgg aaaaagcttc aatgcgatcc ggatgcatcg
     3841 taaaagtcac atctgccttt gcaaggggga tgttgtggca caggggaatc aggttgctgc
     3901 attgtttagc tgccgaaatg cccgccaggg cagcagtggt gagcacagat ccctttttta
     3961 cctggtcttc ctttatcaac tgtatcgtgt cggaagacag ggcaatggag ccgcatgcct
     4021 ttgcctccct caggctgttt tccttggacg aagtatctac catatgcaca cggccgtcct
     4081 tccgggtatg gcttagttcc tgtttcatag attcagattt tttcatcctc ccagataatt
     4141 gaatgtttca cagctgttga aagttccggc cccgggtttt tcaagcaggg ccgtatccag
     4201 ggcttttgct gcacccagtt tgcggatatc gtaacccgta ttgctgaaca ggcagggttt
     4261 cagcattcct gttgaagtga gccgcaggcg gttgcaacgg gggcagtccc cgccatcccc
     4321 gccatctacc cttgtaaaag acccgttttt caggtccatg tattgtatca gtctcagctt
     4381 cagtccttct tcccgggcaa aatcacgcaa cctggccagt tcatcttccc cggtttcagg
     4441 cagcaggacg cagttcagtt tgagtgggga acctgcccct ccaaaaccgg cttttttggc
     4501 tgtcctgata ccctgcagga ccttgttcac gtccccgcca cgggtaagct gcctgtattt
     4561 ttcaggatcc agggtgtcaa ggcttatgtt gagccggtgc aggccggcct ctttcagttc
     4621 caccgccatg gctgtaagca ggataccatt ggtggtcatg gccaggtctt tgatgccttc
     4681 catgccagcc agggaacgta ccagtccggt tatgcctttg cgcaccagcg gttccccgcc
     4741 ggtcaggcgg accttgctga ttccccggct gacagcatac cgggtaaaag ccaggatttc
     4801 ctcataggag agtaactgtt catgcgccac gggaggtacg ccttctgcag gcatgcagta
     4861 gatgcatctg agattgcaac ggtctgtaac cgatatgcgc aggtaattga tttgccttcc
     4921 aaaggagtct gtcatggg
//
LOCUS       DAOSOF010000138         4930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17235_length_4930_cov_4.299897, whole genome shotgun sequence.
ACCESSION   DAOSOF010000138 DAOSOF010000000
VERSION     DAOSOF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17235_length_4930_cov_4.299897"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLM68_07475"
     CDS             <1..264
                     /locus_tag="PLM68_07475"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOT55233.1"
                     /translation="LIMVNNGELVASVPLRSTIIYLRSILSNSTEKANFAYSYDNRVF
                     QRLGNELKMRFSLKVFTGNKFGLFNYATQESGGYVDFDWFTMN"
     gene            273..2735
                     /locus_tag="PLM68_07480"
     CDS             273..2735
                     /locus_tag="PLM68_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOT55234.1"
                     /translation="MKKTCPIICVAFLFLLNNTPANAQQSSGLKLWYDKPAGQWIEAL
                     PIGNGRLGAMVYGDPCLETWQLNENTVWAGQPNHNDNPEAREALPEVRKLIFQEKYQE
                     AQALTDQKFVSKSSHGMPYQTVGNLKIFFPGHDKYSDYYRELDLENATVSSRYKVDDV
                     TYQTRVFSSFPHQVIVGRITSDKPGALHFSVTAAPVPGAHVTTNGDDELVLTGTTSDF
                     EGVTGAVTYCARIRILTSGGSVSACDTVLHVTEADTATVYVSITSNFINYNELGGNAE
                     EKSASYLDQALGTSYERILNEHCADYQQYFNRVHLDLGVTSAENRPTDLRLERFNTDN
                     DPHLVTLYFQYGRYLLISSSRPGGQPANLQGLWNDRLFPPWDSKYTVNINTEMNYWPS
                     EITSLPEMHEPLIRMTSELAQTGKQTARDMYGARGWVLHHNTDIWRINGPVDGSFWGM
                     WPLGGAWLSQHLFDKYLYSGDTEYLEQAYPVIREASRFFLDFLTEEPENHWLVVCPSV
                     SPENAPWTHPEYSITAGATMDNQLVFDLFVKAMEAARILKKDKGLADSILMALDRLPP
                     MQIGKWGQLQEWLYDWDSPDDKHRHVSHLYGLYPSNQISPFRTPELFSAAKTSLAARG
                     DASTGWSMGWKVNLWARLLDGDHAYKLIMDQLSPAVQADGRPRGGTYPNLFDAHPPFQ
                     IDGNFGCTAGIAEMLLQSHDGAVHLLPALPGVWKTGSITGLRARGGFVVDITWKNGIL
                     YSALIRSSQGGICRLRSYVPLTAKGLEKAHGTNPNPFFFVKQVKAPLTHIIQSDSEPL
                     HKRVYEYDILTKKGDVIKINCQ"
     gene            2739..>4930
                     /locus_tag="PLM68_07485"
     CDS             2739..>4930
                     /locus_tag="PLM68_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT55235.1"
                     /translation="MKKLFKVNNILLVAGGLLTLLSACNTSAPCHEKIREVPFTNIHF
                     NDIFWAPRIEINRIVSIPSAFNQCEINGRFDNFAIAGGLTEGEHRGDFSFDDTDPYKV
                     IEGASYSLAARYDPRLDRYLDSVIQIIAAAQEPDGYLTTCVTNKVERLSGWWGSSRWE
                     KINSHELYNSGHMYESAVAHYLATGKRTFLDVAIKNADLICEVFGPGEGQKHVPSGHP
                     IVEMALAKLYKVTGEKKYLDLARYFIDETGRGTDGHKLNAYSQDHMPILQQEEIVGHA
                     VRAGYLYSGVADVASLTNDQEYFKALCRIWDNMAGKKLYITGGIGSRSQGEGFGPGYE
                     LHNHNAYCETCASIANVYWNYRMFLATGDAKYVDVLERALYNGVISGVSLGGDNFFYD
                     NPLESMGQHLRQTWFGCACCPGNITRFMASVPGYLYAVQGKDIYVNLYVQGTAQVETS
                     AGEIILEQVTKHPWEGNIKLIVNPAVKTEFTLKLRIPGWAQDAPVPTDLYSFVNGKGE
                     YAVTINGKKVTGKPQNGYVNIERTWSKGDLVEIEFPMEVRQIRAHEAVADDRGKLAIQ
                     RGPVVFCIEGQDQHDHTVFNKYIPENTLFTTHYDDQLLNGVTVLTGTAMQVGPDGMEE
                     KVPFTAIPYATWNNRGADEMAVWIPQAAEYARPVPEPTIASRAFAATAGRRSDQDGFS
                     TQTGAIEELSVPEFGFNDQWDPINSADRSKPYFYWWLRYGSEESVGYV"
BASE COUNT         1345 a         1129 c         1252 g         1204 t
ORIGIN      
        1 ctgatcatgg tcaataacgg ggaactggtc gcctctgttc cgttacggtc aacaatcata
       61 tacctgagga gcatactgtc aaactccacc gaaaaggcaa actttgcata cagttatgac
      121 aacagggtgt tccagcggtt agggaatgaa ctgaaaatga gatttagtct gaaagtcttt
      181 acagggaata aattcggatt attcaattat gcaacccagg aatcgggggg gtatgttgat
      241 tttgactggt tcacaatgaa ttgaaatcgg gaatgaaaaa aacatgtccg ataatatgtg
      301 ttgcttttct ttttttgctc aataatactc cggccaatgc gcagcaaagt tcaggtttga
      361 aactgtggta tgacaaaccg gcaggacagt ggattgaagc tttacccata gggaacggcc
      421 gcctgggagc gatggtgtat ggggacccct gcctggaaac atggcagtta aacgagaata
      481 ctgtctgggc cggccaaccc aatcataacg acaatccgga agccagggag gccttgcctg
      541 aggtcaggaa gttgatcttc caggaaaaat accaggaggc acaggctctg acagatcaga
      601 aatttgtgag caaatcatcg cacggcatgc cttatcaaac agtgggcaat ctgaaaatct
      661 ttttcccggg acacgacaag tactcagatt attaccgcga acttgacctg gagaatgcca
      721 ccgtctcttc acgttacaag gtggatgacg ttacctatca aacacgtgtg ttttcttctt
      781 ttccacatca ggtgatcgtg ggccggatca catcggacaa acccggagcc ctgcattttt
      841 ctgtgacagc cgctcctgtt ccgggtgcgc atgtgaccac aaacggggat gacgaacttg
      901 tcctgaccgg aacgaccagt gattttgaag gagtaacagg agccgttacc tattgtgcac
      961 gtattagaat cctgacgagt ggaggatcag tgagcgcctg tgataccgtg ttgcatgtta
     1021 cggaagccga cacagccacc gtttatgttt ccataacctc gaatttcata aattacaatg
     1081 aacttggagg gaacgccgaa gaaaagtcgg cctcatacct ggaccaggct ttgggtacga
     1141 gttatgaacg tatcctgaat gaacattgtg cggattatca gcagtatttt aaccgggtgc
     1201 accttgatct tggagtaact tcagcggaaa ataggccaac ggatctacgg ctggaaaggt
     1261 tcaacacaga taatgatccg catcttgtca ccttgtattt ccagtatggg cgttatttgc
     1321 tgatctcttc ctccaggccc ggaggccagc cggccaattt gcagggttta tggaacgacc
     1381 ggttgtttcc tccttgggac agcaagtata cggtaaacat taataccgaa atgaattact
     1441 ggccttccga gatcaccagc cttcccgaga tgcacgagcc gctgatacgc atgacaagcg
     1501 aactggcaca gacaggaaag cagacagccc gggacatgta tggtgcacgg ggatgggtac
     1561 ttcaccacaa taccgatatc tggagaatca acggtccggt tgacggctct ttctggggca
     1621 tgtggccttt ggggggagcc tggctttcgc agcatttatt cgacaaatac ctgtacagcg
     1681 gggatacgga gtacctggaa caggcatatc cggttatccg ggaggcttcc cgcttttttc
     1741 ttgatttttt gactgaagaa ccagaaaacc actggctggt agtttgtcct tctgtctccc
     1801 cggaaaatgc gccctggaca catccggaat attccataac ggcaggagca accatggaca
     1861 accaactggt atttgatctg tttgtaaaag ccatggaagc tgcgcgtatc ctgaaaaagg
     1921 acaaaggtct ggcagacagc attctaatgg cccttgaccg tctgccaccc atgcagatcg
     1981 gtaaatgggg acaattgcag gaatggctct atgactggga cagtcctgat gacaagcata
     2041 gacatgtttc acatttgtac gggctatatc cttccaacca gatatcaccc ttcagaacgc
     2101 cggagttgtt ttcggccgca aaaacctctt tagcagccag gggagatgca tccacgggct
     2161 ggtccatggg gtggaaagta aatttatggg ccaggctgct ggacggggat catgcgtata
     2221 aactgattat ggatcagctg tctcctgctg ttcaagccga tggaagacca cgtggaggaa
     2281 cttatcctaa cctttttgat gcccatccac catttcaaat tgacggcaat tttggctgta
     2341 cagccggtat tgccgaaatg cttctccaga gccacgacgg agcggttcat ttgttacccg
     2401 cacttccagg tgtctggaaa accggctcta tcacgggatt gagggcacgc gggggattcg
     2461 tggtagatat aacatggaaa aacgggatcc tgtactcggc acttatcagg tcttctcagg
     2521 gaggtatttg ccgcttacga tcctatgtcc cgttgacggc aaaaggcttg gaaaaggcac
     2581 acggcacaaa tcccaatccg ttcttttttg tgaagcaggt aaaagcccca ttgacacaca
     2641 taatccaaag tgactctgaa ccattgcaca aaagggtata tgagtatgat atccttacaa
     2701 aaaaaggaga tgtcattaaa attaattgtc aataaaccat gaagaagtta ttcaaggtga
     2761 ataatatcct gttggtagca ggaggactgc tgaccctgtt gtccgcatgc aatacttcag
     2821 ctccatgcca tgaaaagatc cgggaggttc cttttacaaa tattcatttt aatgatattt
     2881 tctgggctcc cagaatcgag ataaacagga ttgtttccat accttctgca ttcaaccaat
     2941 gtgaaataaa cggacgtttt gacaactttg ctattgccgg tggattgaca gaaggagagc
     3001 atcgcggtga tttttccttt gacgataccg atccatataa agtgattgaa ggggcatcgt
     3061 actccctggc cgccagatat gatcccaggc tggaccgtta cctggacagc gtcatacaga
     3121 taatagccgc tgcccaggaa cctgatgggt acctaaccac ctgtgtaacc aacaaagtgg
     3181 aaaggttgtc ggggtggtgg gggtcctccc gctgggagaa gatcaacagc catgaattgt
     3241 ataacagcgg acacatgtac gagtctgctg tggctcacta tcttgcaaca ggaaaaagaa
     3301 ctttcctgga tgtagccatt aaaaatgccg acttgatttg tgaagttttc ggtccggggg
     3361 aaggacaaaa acatgttccc tcgggacatc ccatagtaga aatggcactg gccaaactgt
     3421 ataaggtaac gggagagaaa aaataccttg acctggccag atatttcata gacgaaacag
     3481 ggcggggaac cgacggacac aaacttaacg cttacagcca ggaccacatg cccatcctgc
     3541 agcaggaaga gatcgtcggt cacgcagttc gtgcaggata cctgtattcc ggggtagccg
     3601 acgtggcatc gcttacaaac gaccaggaat actttaaggc tttgtgtcgt atctgggata
     3661 atatggcagg taaaaagctt tatatcacag gaggaattgg ctctcgctca cagggagaag
     3721 gattcggtcc cggttatgag ttacacaacc ataatgccta ttgtgaaaca tgcgcttcca
     3781 ttgccaacgt gtactggaat taccgtatgt tcctggctac aggggatgcc aaatacgtgg
     3841 atgtactgga acgtgctttg tacaacggcg taatttccgg ggtctcacta ggaggagaca
     3901 attttttcta tgacaatccg ctggaatcca tggggcaaca cttgcgtcag acctggttcg
     3961 ggtgtgcctg ctgcccgggg aatattaccc gctttatggc ttctgttccc ggttatttgt
     4021 atgccgtgca gggaaaagac atttatgtga acctctacgt tcaggggact gcacaggtag
     4081 agacatcggc aggagagatt attcttgaac aggtgaccaa acatccctgg gaaggcaata
     4141 tcaaacttat cgtaaatcct gctgtaaaaa cagaattcac actgaaactc cgtataccgg
     4201 ggtgggctca ggatgctccc gttcctaccg atctctactc ctttgtaaac ggaaaaggag
     4261 agtacgcggt taccataaat ggaaagaagg tgaccgggaa accccaaaat ggttatgtaa
     4321 acattgaaag gacatggtct aagggggatc tggttgaaat tgagtttccc atggaagtac
     4381 gtcagatcag ggcacatgaa gcagtggctg atgacagggg aaaacttgca atccagcgcg
     4441 gtcccgttgt attttgcata gaaggacaag accagcatga ccacacggtg ttcaacaaat
     4501 acatacctga aaacactctt tttacgacac attacgacga tcaactgctc aatggcgtta
     4561 cagtgcttac aggtactgca atgcaggtag gaccagacgg tatggaagaa aaagtgcctt
     4621 ttaccgccat tccttatgct acctggaata accgcggcgc agatgaaatg gccgtctgga
     4681 tcccccaggc tgccgaatat gcccgtcctg taccggaacc taccattgca tcccgtgctt
     4741 ttgctgcaac cgcaggacgc agaagcgacc aggatggttt ttccactcag acaggagcta
     4801 ttgaagaact atctgtccca gagttcgggt ttaacgacca gtgggatccc atcaactcgg
     4861 cagatcgttc caagccgtat ttttactggt ggttgcgcta tggaagtgaa gaatctgtag
     4921 gttatgtgtt
//
LOCUS       DAOSOF010000139         4895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17395_length_4895_cov_5.737810, whole genome shotgun sequence.
ACCESSION   DAOSOF010000139 DAOSOF010000000
VERSION     DAOSOF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17395_length_4895_cov_5.737810"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1127
                     /locus_tag="PLM68_07490"
     CDS             120..1127
                     /locus_tag="PLM68_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT55236.1"
                     /translation="MNIALVYGGNSSEREISILSGKHMAGHLLAGDRAVFEIDIYGDR
                     WKLVAVNGQDIPPADVDKNGFTVPWQSERISFDVVVLMIHGTPGEDGLFIAYLEMMGI
                     PHTACPARTALLAFDKLACKAYLRGNGINMAREFRVTEQTLAGPWQQQAARALGPMPW
                     FVKPVAGGSSFGVSRVSEPSELLPAVEYAMKEDHVVMIEEFIQGIEVTNGIMKTAERE
                     FLLPVTEIIPGNGCEFFDYKAKYEGASMEITPARITPEMTSLVQQTTSGIYDNLMCRG
                     LIRVDYILRGTDLYFLEVNTLPGMTQKSLVPQEILASGMTLREFMDHLIRSAIDEHKR
                     I"
     gene            1108..1971
                     /gene="prmC"
                     /locus_tag="PLM68_07495"
     CDS             1108..1971
                     /gene="prmC"
                     /locus_tag="PLM68_07495"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT55237.1"
                     /translation="MNIKEFKDYAREHLAPSLEKPELRFLVSALLAHFAGIPNYWYHT
                     HEDHLLSPAVAGELLQAVDQAVQGRPLQYILGFVHFGNCRIAVDERVLIPRPETEEMW
                     DRARHIHGVVLDACTGSGCLAIALKKDRPGTSVHAFDLSPAALEVATGNALLNQTEVH
                     FFQADLSCIDGVEKAAAEAGLEKHTVGLLISNPPYVTESQKKGMSRRVLSYEPHSALF
                     VPDSDPLCHYRQLALLGNRFLCHGGTLMAEINEDFGREVCLLMEQAGYAAVELHRDLH
                     EKNRIISARWV"
     gene            2164..3459
                     /locus_tag="PLM68_07500"
     CDS             2164..3459
                     /locus_tag="PLM68_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT55238.1"
                     /translation="MKTTKKFLLPDQEIPTAWYNVLADMPPLQPMLNPATKEPVKPSD
                     LEPLFAKGLIEQEFSTERWIPIPEEVRTLYKIYRPTPLVRASGLEKALDTPAHIYFKN
                     ESVSPVGSHKLNTALPQAYYNKIEGVKNLATETGAGQWGSALSIACSHFGLNTEVFMV
                     RLSYDQKPYRKVVMQTYGAKVIPSPSNLTKAGQKFLAEKPHTNGSLGMAISEACERAV
                     TTPDTRYTLGSVLNMVMLHQTIIGLEAERQFEMAGDYPDTIIGCFGGGSNFGGISLPF
                     LRHQLAGKAGFDFIAAESSGCPKLTKGTFRYDHGDTAGMTPLIPMYTLGCEYEPAAIH
                     AGGLRYHGAGAIMSQLLKDKIIRAVAIDQDECFKVGILFSRCEGIIPAPESTHAIAAT
                     VREALKAKEEGREKVILFNLSGHGLLDLSAYESFLNGKL"
     gene            complement(3443..4234)
                     /locus_tag="PLM68_07505"
     CDS             complement(3443..4234)
                     /locus_tag="PLM68_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT55239.1"
                     /translation="MKKTLIFWLLAALYFPNPAHAQEKAPIIGLSATYETSQNCVPNT
                     YIISVRKAGGIPVILPVTDDPAIIARMVESIDGLILTGGEDVDPLRWYGEEPIPGMGG
                     MVPVRDFFDIHLARAAVERGIPLLGICRGEQVLNVAFGGTLYQDIPSQVRSVNLVKHR
                     QNAPREYGTHTITVNRGTTLYGILKEVLEDGDTYRVNSFHHQSVKDVAPGFVVSATAP
                     DGIVEGIEMKGNPNVIGVQFHPEGPVSEGDDALLPLFLQLVKASR"
     gene            complement(4231..>4895)
                     /locus_tag="PLM68_07510"
     CDS             complement(4231..>4895)
                     /locus_tag="PLM68_07510"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HOT55240.1"
                     /translation="DTLLSAAQMDYLHMGQLITSQTDTKTNIYAPCWFVEQNNRYRVW
                     FHTGTTWGFTAICAFVPELDLGLMWLSNCEPPSDPRYAIMRHVIDYYMDLPFQDYSRQ
                     ALEKFLAEALERENKEKKEQASRVAEPPAPYPHYVGTYTHQAMGEAVITLENGNLYIT
                     LGPEDFPNRKHLMTHVTGETFRFRSGGSAFTMKFAMEDHLLTFDLDLHQNENMGLWRA
                     RK"
BASE COUNT         1173 a         1303 c         1332 g         1087 t
ORIGIN      
        1 ggattctctg tggaagacca gtttgtggcg cctcccggat ttgacatatc cctggaatgc
       61 gacggctccg accaggatat ggaccagcaa cgccgcacgg aaggtttttt caattgatta
      121 tgaatattgc ccttgtatac ggaggcaact cctccgaacg cgaaatttcc attttaagcg
      181 gaaagcacat ggccggacac ttattggcgg gtgaccgtgc tgtttttgaa atagatatct
      241 acggggaccg gtggaaactg gttgccgtca acggacagga tattcctccg gccgatgtgg
      301 acaaaaacgg attcacggta ccctggcaaa gtgaacgcat atcttttgat gtggtggttc
      361 ttatgatcca cgggactccg ggagaagacg gcctgttcat tgcctatctt gaaatgatgg
      421 ggatacccca tacagcttgc ccggcacgta ctgcccttct ggcttttgac aagctggcct
      481 gcaaagcgta tctgcgcgga aacggcataa acatggcccg tgaattccgt gtcacggaac
      541 aaacgctggc aggaccgtgg caacagcagg ctgcccgggc actggggccc atgccctggt
      601 ttgtaaaacc tgtggcagga ggttccagtt ttggtgtctc cagggtgtct gaaccttctg
      661 aactcctgcc tgccgtggag tatgccatga aagaagacca tgtggtcatg atagaggaat
      721 tcatccaggg gattgaagtg accaacggga tcatgaagac ggcagaaaga gaattcctgc
      781 ttcccgtgac ggagatcatc cccgggaacg ggtgcgaatt tttcgattac aaagccaagt
      841 acgaaggggc atccatggag atcacacccg cccggatcac ccccgagatg acaagccttg
      901 tgcagcagac cacatccggg atatacgaca acctgatgtg caggggcctt atccgggtgg
      961 attatatcct gcgcgggaca gatctttatt ttctggaggt caacaccctt ccgggcatga
     1021 cgcaaaaaag cctcgtaccc caggagatcc tggcgtccgg catgaccttg cgggagttca
     1081 tggaccacct gatccgttct gccatagatg aacataaaag aatttaaaga ttatgcacgg
     1141 gaacacctgg ccccttcgct ggaaaagccc gaactgcgtt tcctggtaag cgccctgctg
     1201 gcgcattttg ccggcattcc caactactgg taccatacac atgaagacca cctgctctca
     1261 ccggcagttg ccggggaact gctgcaggcg gtggaccagg ccgtgcaggg caggcccctg
     1321 caatacatcc tgggttttgt acatttcgga aattgccgca ttgccgtgga tgagcgggta
     1381 ctcattccca ggccagagac ggaagagatg tgggaccgtg cccggcatat ccacggcgta
     1441 gtgctggacg cctgtaccgg aagcggatgc ctggcaatag ccttgaaaaa agaccgtccc
     1501 ggaacaagcg ttcatgcctt tgacctgagt cccgccgcct tggaggtggc cacagggaat
     1561 gccctcctga accagacgga agtgcatttt ttccaggcag acctgtcctg cattgacggg
     1621 gtggaaaagg ccgcagcgga agcaggcctg gaaaaacaca cggtcggcct gctcatatcc
     1681 aatcccccgt atgttacgga aagccagaaa aagggaatgt cccggagggt actttcctat
     1741 gaaccccaca gtgcgctttt tgtgcctgac agcgacccgc tgtgccatta caggcaactg
     1801 gccctgctgg gaaaccgttt cctctgccat ggcggcaccc tgatggcaga gatcaatgaa
     1861 gactttggac gtgaagtctg cctactcatg gagcaggccg gatatgcagc agttgagttg
     1921 cacagggacc tgcatgaaaa aaacaggatt atttcagccc ggtgggtatg atgaaatgtt
     1981 ttttttatta tatttgtgac aacatgagaa aaattgctat gatcaacaga tggtggtggc
     2041 tcggagaata tcttttccgc gtcgcatctg tggcatagta accataacct ttattattaa
     2101 ccaaaagtgc cctcttgcaa cgcaaggggg ctttttttat tattttttta aaagaaaaaa
     2161 gctatgaaaa caaccaagaa gttccttctt cccgaccagg aaattcccac ggcatggtat
     2221 aacgtgctgg ccgatatgcc gccattgcaa cccatgttga acccagctac aaaagaaccc
     2281 gtcaaacctt cggaccttga gcccctgttt gccaaaggac tcattgaaca ggaattttcc
     2341 acagaacgat ggatccccat tccggaagag gtacgcacgt tatataagat ataccgccca
     2401 acccccctgg tccgcgccag cggcctggaa aaagcgctgg acaccccggc acatatctat
     2461 ttcaagaatg aaagcgtttc ccccgtggga tcccacaaac ttaacacagc ccttccccag
     2521 gcctattata ataaaataga gggagtcaag aatcttgcca cagaaacagg agccggtcaa
     2581 tgggggtctg ccctgagcat tgcctgcagc cattttggcc taaacacgga agtattcatg
     2641 gtcaggctca gctacgacca gaaaccatac cggaaggtgg tcatgcagac gtacggcgca
     2701 aaagtaatcc cttctcccag caaccttacc aaggcaggac agaagttttt ggcagagaaa
     2761 ccccatacca acggatcgct gggcatggcc atatcggaag cctgcgaaag ggccgtgacc
     2821 acacccgaca cccgttatac attgggcagc gtcctgaaca tggtcatgtt gcaccagacc
     2881 atcataggac tggaagcaga gcgccagttc gagatggccg gggattatcc cgacaccatc
     2941 atcgggtgtt tcggcggagg ctccaatttc gggggtataa gcctgccttt tctacgtcac
     3001 cagcttgccg gaaaagccgg gtttgatttc atagcggccg aaagctcggg ttgtcccaaa
     3061 ctgaccaaag gcaccttccg ttacgatcac ggcgatactg cagggatgac accgctcatt
     3121 cccatgtaca ccctgggctg cgaatacgaa cccgcggcca tccatgcggg gggattgcgt
     3181 tatcacggcg caggggccat catgagccag ttgttgaagg acaagatcat ccgtgcggtg
     3241 gccattgacc aggacgaatg ctttaaggtg gggatccttt tttcccgttg cgaagggatc
     3301 attcccgctc ctgaatcgac ccacgccatt gcggcaaccg ttcgggaagc cctcaaggca
     3361 aaagaagaag gccgtgaaaa ggtcatcttg ttcaaccttt ccggtcacgg cctcctggac
     3421 ctgagcgcct acgaatcttt tctcaacggg aagctttgac cagctgaagg aacaggggaa
     3481 gcagcgcgtc gtcaccctct gagacgggac cttcgggatg gaactgcacc ccaatgacat
     3541 tggggttgcc tttcatctcg atgccttcca cgatcccgtc gggggctgtg gcagaaacca
     3601 caaagcccgg cgcaacgtct ttgaccgact ggtggtggaa ggaattgacc cggtatgtgt
     3661 ccccgtcttc cagcacctct ttgaggatgc cgtagagcgt ggtaccccgg ttcaccgtta
     3721 tggtatgggt gccgtattcc cggggagcgt tctgccggtg ttttaccagg ttgaccgacc
     3781 tgacctggga agggatgtcc tggtataatg tgcctccgaa agccacgttc aggacctgtt
     3841 cccctcggca gatgcccagc aagggtatcc cgcgctccac agccgcacgg gccaggtgaa
     3901 tgtcaaagaa atcccgtacg ggaaccatcc ctcccatacc agggatgggt tcttccccgt
     3961 accagcggag ggggtccaca tcttccccgc cggtaagtat gagcccgtca atggattcca
     4021 ccatacgcgc tatgatggcc ggatcgtctg tcaccggaag tatcacaggg atacccccgg
     4081 ctttccggac tgaaatgata tacgtgttgg gcacgcagtt ctgggaagtc tcgtacgtgg
     4141 cagaaagccc tatgatgggt gccttttcct gtgcatgtgc aggattcgga aaatacaagg
     4201 cggccagtag ccagaagatc aatgtttttt tcatttgcgg gccctccaga gtcccatatt
     4261 ttcattttga tgtaaatcca ggtcaaaagt caggaggtga tcctccatcg cgaatttcat
     4321 ggtaaaggca gatcctccgg aacggaaacg gaatgtttca ccggtgacat gggtcatcag
     4381 gtgtttccgg tttggaaaat cttccggtcc cagggtgatg tacaaattac cgttctccag
     4441 ggtgatgaca gcttctccca tggcctggtg ggtgtatgtc cccacataat gggggtaggg
     4501 agccggaggt tcggcaacac gggaagcctg ttcctttttt tctttatttt ccctttccag
     4561 ggcctcggcc aggaattttt cgagcgcctg gcggctgtaa tcctggaaag gcaggtccat
     4621 gtaatagtca atcacatggc gcataatggc atagcgagga tcggaagggg gttcgcaatt
     4681 gctgagccac atgagcccca ggtccaactc cgggacaaag gcacaaatgg ctgtgaagcc
     4741 ccaggtggtc ccggtatgga accagacacg gtaccggttg ttttgttcca caaaccagca
     4801 gggggcataa atattcgttt ttgtatccgt ctgtgacgtg atgagctggc ccatgtgcag
     4861 gtaatccatc tgggcagcag acaacaaggt gtccc
//
LOCUS       DAOSOF010000140         4828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_17650_length_4828_cov_4.751938, whole genome shotgun sequence.
ACCESSION   DAOSOF010000140 DAOSOF010000000
VERSION     DAOSOF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17650_length_4828_cov_4.751938"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="PLM68_07515"
     CDS             complement(<1..1290)
                     /locus_tag="PLM68_07515"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOT55241.1"
                     /translation="MKKSQYKPVIILAGAVLLLSILLVLMFYIWEIPVFQWLKSDRPE
                     IFLESNYGLRNQKGRVIVDPTYDSIAVFSEIAVLQRGSEYGVSNKRGKMIIPMQTRPI
                     TNEYHGQRLPFLLALRSDGSFTLIDHKGKEREIPVYDSLELREDDIFICRDGLWGVLD
                     GDGFEQVPLIYDQIVKRAGNYFIVRKGNYEGLHSKGSKEPLVPVMYDKVDECTSLTFR
                     VLLDDHYGLLDHDAVSLIETVYDTIYFHHSPEWIVTGDSGRYSLRNTTNLEYPAFFYD
                     IIGPVREGMTVVRNENGYGVLSSDGKVVVQPGYDLIRDYSNGLAIVMQNQQYGVIDRY
                     GNFTLPFSLNLVEIYDFHNELALAARFNMMEARIQKQFGFIDKKGNTIIPFIYQDGHR
                     MFSNGLAGVKFNGLWGFINKKGESMIPFIYDSVSVFSH"
     gene            complement(1271..2065)
                     /gene="tatC"
                     /locus_tag="PLM68_07520"
     CDS             complement(1271..2065)
                     /gene="tatC"
                     /locus_tag="PLM68_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT55242.1"
                     /translation="MTFWEHLEEFRKVIFRSAIVLILLMVVVFVNKNFVFDAVIFAPR
                     SSDFILYRWMTALSGLVGISSISPKPFTLQLQNIELSAQFFTHISVSFSIAFVLATPF
                     ILYQLWIFIQPALYLKEKKTVKGAFGWCSLLFFLGVLTGYFFVFPLTINFLGSYQVSE
                     WVDNQITLQSYIHMFTWLVIIMGIVFELPILLRVLSKLGVVSKELLKKHRKHAVVILV
                     ILAAIITPSGDAFTLLVVALPLYLLYEVSILVSFSKRATEDEKVAV"
     gene            complement(2098..2307)
                     /locus_tag="PLM68_07525"
     CDS             complement(2098..2307)
                     /locus_tag="PLM68_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOT55243.1"
                     /translation="MCKLLLFGTLGATEIIIIALIVLLLFGGRKIPELMKGIGKGVRS
                     FRDGVKGIEDDLKEDEKESDQDIKK"
     gene            complement(2300..2737)
                     /locus_tag="PLM68_07530"
     CDS             complement(2300..2737)
                     /locus_tag="PLM68_07530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT55244.1"
                     /translation="MMEHTTQITVRYYETDQMGVVHHSNYIRYFETAREEMMAHFGVP
                     YTFSESQGIIMPVRVVHCDYIHPARYGQVLTVITTVKEMPRSRITYHYQVFNDTSVLL
                     ATGYVTLAFVDASRSVPVRAPRFLTEVWEKYLNLNKTKQQENV"
     gene            complement(2740..>4828)
                     /locus_tag="PLM68_07535"
     CDS             complement(2740..>4828)
                     /locus_tag="PLM68_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HOT55245.1"
                     /translation="WEDKRTFYLVDILSRQARAWELVWQQWLNPANLLESFVIGEYSS
                     QNNQIRLGEPRIDTLNSLVEIPLVEGNDFPLQITSRQVTLSEDATYDLPPVLTFESYR
                     TVVPFSVTSQSGNRRDWIMRLDPWFETEARILSFVVDSYNSRENLVKLESNTATIDPD
                     TRTVTLVLKSGYDFPLILNAFSMELSPKATLQETFPEGILFPAINDIISLTIVAESGD
                     VTEWKLQLADERTENLEALVMDYHITGYQGTSQTEHNISLLEEGVVDSLNHTVTLVIS
                     DWSSKMPLTVDGVLNVSKNAVLGGEITTRQHRVVFNSTGQKYRFSVTSESGTNTTSWT
                     LQLEDRSPVRRSDAAVTDFITGNPSSGFEFDQKFLEPDDGLITLLVKSKSSEELLLTI
                     KPKISISQGARLLGIISGAPLNLSFTEPFSFQVMAEDETVRSWKIQLIFAPQVPNSGF
                     EEWGTIRDIFNILPSNGKGWTTGNNNQISGSFRVQGHNSPYAVQMITQLKTVNLWVMK
                     VTSLAAGALFLGEFNFSMDVEKVLNPSIMTDFGISFAPGSNPVGFEIDYLYEPGGQRV
                     FTEPYKVKIGLFDMPAFKDPVKVSGPDKAVITAELHHNTTGTWKYDLNKRQDMIAENE
                     IATTGTQGWVHERLVFNPVPGRENLPMTHLVVRMSSSWEGAEYKGADGSKLTVDNFRL
                     IYYLPGPTAIILE"
BASE COUNT         1289 a         1175 c         1076 g         1288 t
ORIGIN      
        1 atgcgaaaag acagacaccg aatcatatat gaatggaatc atggattcac cctttttgtt
       61 gatgaatccc cacaatccgt tgaatttcac acctgccagt ccatttgaga acatacggtg
      121 gccatcctgg tatatgaatg gaataatggt attgcctttt ttatctatga agccgaactg
      181 tttttgtatg cgtgcttcca tcatgttaaa acgggctgcc agggccagct cgttatgaaa
      241 atcgtagatt tccaccaggt tcaaggaaaa aggcaatgtg aaatttccat acctgtcaat
      301 caccccatat tgttggttct gcatgactat ggcaagcccg ttgctataat cccggattaa
      361 atcgtaacca ggctgaacca ctacttttcc atctgacgat agtactccat atccattttc
      421 attgcgtaca acggtcattc cttccctgac aggaccaata atgtcgtaaa aaaaggcagg
      481 atactccagg ttcgtcgtat tacgaaggga ataacggcca ctgtcccccg ttacgatcca
      541 ctccggactg tgatggaaat aaatggtatc gtataccgtt tcaatcaggg aaaccgcatc
      601 atggtccagg agtccgtaat gatcgtccag caacactctg aaagtaaggg aggtgcattc
      661 atccactttg tcatacatga caggtacaag gggttcttta cttcctttgc tgtgaagccc
      721 ctcataattt cctttcctaa ctatgaaata attcccggca cgtttcacta tctggtcgta
      781 aataagtggt acctgctcaa agccatcccc gtcaagcacg ccccatagac cgtcccggca
      841 aatgaaaata tcgtcctctc ttaactcaag actatcgtaa acaggtattt cacgttcttt
      901 ccctttatgg tcaattaggg tgaaggatcc gtcagagcgc agcgccagca gaaaaggcaa
      961 ccgttgtcca tggtattcat tggtaattgg acgtgtctgc atgggtatga tcattttacc
     1021 acgcttgttg gacacaccgt actcactgcc cctttgcagg actgcaattt cagaaaatac
     1081 agctatggag tcgtacgtag gatcaacgat caccctgcct ttctggttgc gcaagccgta
     1141 attggattcc aggaaaatct ccggcctatc acttttaagc cactggaata caggaatttc
     1201 ccagatataa aacatcagga ccagcagaat tgataaaagg aggactgcac ctgccaggat
     1261 aatgaccggt ttatactgcg actttttcat cttctgtggc cctcttactg aaacttacca
     1321 atatactgac ctcatataac aggtataaag gcaatgctac caccaacaag gtaaaggcgt
     1381 ctccgctggg tgttatgata gctgcaagaa tgacaaggat cactacggca tgtttcctgt
     1441 gttttttgag caattccttg gagacaacac ctaatttgga taagacgcgc aacaggattg
     1501 gcagttcgaa gacgatgccc atgatgatga ccagccaagt aaacatatgg atataggact
     1561 ggagtgtaat ctggttatcc acccattcgc taacctggta acttcccagg aaatttatgg
     1621 tcagaggaaa gacaaaaaag taaccggtca ggacaccaag gaagaataac agcgagcacc
     1681 acccaaaagc cccctttacg gttttttttt cctttaggta caaagcgggc tgtatgaata
     1741 tccataactg gtacaatatg aacggtgtgg ccaagacaaa ggctatgctg aaagaaacgc
     1801 taatatgggt gaaaaactgg gctgaaagtt ctatgttttg cagttgcagg gtaaaaggtt
     1861 tcggtgaaat ggaagaaatt cccacaaggc cagacagagc tgtcatccac ctgtagagaa
     1921 taaaatcaga gcttctggga gcaaatatga ctgcatcaaa aacaaagttt ttattcacaa
     1981 aaacaaccac catgagtaga atcaggacaa ttgccgagcg aaatatcacc ttgcggaact
     2041 cttcaagatg ctcccaaaag gtcatctcat ttttatgccc cgaaatatct gccaccgcta
     2101 ttttttaata tcctgatcgc tttctttttc atcttccttt aagtcatctt ctatcccctt
     2161 cacaccatcc ctgaaacttc tgacgccttt tccaatgcct ttcataagtt cgggaatctt
     2221 gcgccctcca aagagaagca gtacaattag ggcaataatg atgatctcgg tggcacccaa
     2281 cgtgccaaat agcaataatt tacacatttt cctgttgttt agttttgttc aaatttaggt
     2341 atttttccca tacttccgta agaaaacggg gtgccctcac gggaacactg cgggaagcat
     2401 ccacaaaggc aagggtaaca tatcccgtgg ctaaaagtac actggtatcg ttaaacacct
     2461 ggtaatggta cgtgatcctg gaacggggca tttctttcac tgtcgttatc acagtcagca
     2521 cctggccata acgtgccgga tgtatgtagt cgcaatgaac cacgcgtacc ggcataatga
     2581 ttccctgcga ttccgaaaaa gtgtaaggaa caccaaaatg ggccatcatt tcttcgcggg
     2641 ctgtttcaaa atagcggatg tagttggagt ggtgtaccac acccatctga tccgtttcgt
     2701 aatacctgac ggtaatttgt gtagtatgtt ccatcatgat tattccagta ttatggccgt
     2761 tggcccggga agatagtaaa ttaatctgaa attgtccacc gtgagcttgc tgccatcggc
     2821 acctttgtat tcagctcctt cccaggaaga cgacatgcgt acgaccaagt gagtcattgg
     2881 caggttttcc ctgcccggaa ccggattgaa aaccagccgc tcatgcaccc atccctgggt
     2941 ccccgttgtg gcaatctcat tttcggcaat catgtcctgt cttttgttca gatcatactt
     3001 ccaggttccc gtcgtattat gatgcaattc tgccgtaatg acggccttat cgggtccgct
     3061 tactttcacc gggtccttga atgcaggcat gtcaaacaaa cctatcttca ctttataggg
     3121 ctctgtaaaa acgcgttgcc caccaggttc atacaggtag tcaatttcaa atcccacggg
     3181 attggaaccg ggtgcaaacg aaattccaaa atccgtcatg atggaaggat tcaacacctt
     3241 ttcaacgtcc atgctgaaat tgaattctcc caggaaaagc gccccagcgg ccaggctggt
     3301 caccttcatc acccacaggt taacggtttt caactgggtg atcatctgca cggcataggg
     3361 actattgtgc ccctggaccc tgaaagaacc cgaaatttgg ttattgttcc cggtggtcca
     3421 tcctttgccg ttggaaggca ggatattaaa aatatcacgg atagtacccc attcttcaaa
     3481 accgctgttg ggcacctggg gggcaaagat gagctgtatc ttccaggagc ggacagtttc
     3541 gtcttctgcc ataacctgaa aagagaatgg ctctgtgaaa gacaaattca ggggtgctcc
     3601 ggatattatg cccaaaagcc gtgctccctg gctgatggaa atttttggtt tgattgtcag
     3661 cagtaactct tcggacgatt ttgatttgac cagcaacgtg atcaggccat cgtccggttc
     3721 caggaatttc tggtcaaatt caaagcccga ggaagggttt ccggtaatga agtccgtcac
     3781 cgcagcatct gaacgcctta ccggggaccg gtcttccagt tgtaaagtcc agctggttgt
     3841 attggttccg ctttcggaag taacagaaaa ccggtacttc tggccggttg agttgaaaac
     3901 caccctgtgc tgccttgtgg tgatctcccc gccgagcaca gcgttctttg atacgttcag
     3961 caccccgtcc acggtcaggg gcatctttga ggaccagtcg ctgataacca gcgtcaccgt
     4021 atgattaagc gaatctacaa ccccctcttc caggagcgag atgttgtgtt ctgtctggct
     4081 ggtgccctgg tagcctgtaa tatggtaatc catcaccagg gcttccaggt tttcagtacg
     4141 ttcatctgcc agttgcagct tccattctgt cacatcgccg ctttcagcta ctatggtcaa
     4201 agagataata tcattgatgg ccgggaatag aattccctcc gggaatgttt cctgcagggt
     4261 agctttgggc gataattcca ttgaaaaagc gttcagaatc aatggaaaat catatcctga
     4321 ctttagcacc agtgttacgg tacgggtgtc cggatctatg gttgcagtgt tgctttccag
     4381 cttaaccagg ttttccctgg aattgtaact atccaccaca aacgagagta tccgggcctc
     4441 tgtttcaaac cagggatcta gcctcatgat ccagtcacgg cggttccccg attgggatgt
     4501 tacggaaaac ggtaccacgg tgcggtaaga ttcaaaagtc aataccggtg gcaaatcata
     4561 ggtggcatct tcggataatg ttacctggcg ggatgtaatc tgcagcggaa aatcattgcc
     4621 ttcaaccaag ggaatctcca ccagggaatt gagcgtgtct atccggggtt cacccagccg
     4681 gatctggtta ttctgtgagg aatattcccc aatcacgaat gattccaaca gattggccgg
     4741 attgagccac tgctgccaca ccagttccca ggcccgtgcc tgtcgtgaaa gtatatcaac
     4801 caggtaaaag gttcttttat cttcccag
//
LOCUS       DAOSOF010000141         4696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_18269_length_4696_cov_4.023702, whole genome shotgun sequence.
ACCESSION   DAOSOF010000141 DAOSOF010000000
VERSION     DAOSOF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18269_length_4696_cov_4.023702"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLM68_07540"
     CDS             <1..820
                     /locus_tag="PLM68_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT55246.1"
                     /translation="VIMPKLLEALGVTCYKIHSEPTGDFAHNPEPLPHHLKDLSSEVV
                     ARKATLGISVDPDVDRLALVCQDGSMFGEEYTLVACADYVLGKYAAQYPDKKLHTVSN
                     LSSTRALKDITQRYNGSCSAAAVGEVNVVEEMKRTGSVIGGEGNGGVILPSLHYGRDA
                     LIGTALVLSLLAEKGQSMTGLRSSYPDYHMSKNRVDLKEDMSLEDLSDRIIKAYPTAT
                     VNNRDGLRLDFENEKKWVSLRKSNTEPILRIYSEAPDKTGAESLASEIMSLLSL"
     gene            889..1140
                     /locus_tag="PLM68_07545"
     CDS             889..1140
                     /locus_tag="PLM68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOT55247.1"
                     /translation="MKQGIHPETYRLVAFKDMSNDYVFVTRSCVNAKETITLEGVEYP
                     LYKLEISNTSHPFYTGKMKLVDTAGRVDKFRSRYGDRKK"
     gene            1121..1897
                     /gene="mnmD"
                     /locus_tag="PLM68_07550"
     CDS             1121..1897
                     /gene="mnmD"
                     /locus_tag="PLM68_07550"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOT55248.1"
                     /translation="MAIVKNNTAQHFTKSCPRKDGFFDDISLQPVPTEDGSNTLMGPW
                     GETYHSISGAIQESQHVYIISGLLYYLNSQVSEGRLPHAIHILEVGFGTGLNALLTYR
                     AMNSCIFPKATPVYYETVEMFPLPENITNELLYPDSPREKEFFRNIHRGKWGEPFQAA
                     EGFTMLKQKGNILDYQPQEGIHVVYYDAFSPGVQPELWSRNVFAGITPSLEKAAVLTT
                     YCCRGMVKNALRELGFLIERLPGTGRKRHILRAVYCMQSC"
     gene            complement(1876..2526)
                     /locus_tag="PLM68_07555"
     CDS             complement(1876..2526)
                     /locus_tag="PLM68_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOT55249.1"
                     /translation="MKHLFVLSLFISCFFITHAQEHTEQPIRPDAVFMGNSITQNWRN
                     FHPDFFTRNNFVGKGIGGEVTLQMLARFRRDVISLNPKVLVILAGTNDIAQNQGYVTH
                     RQIMDNIASMVDLAHFNNIRVILCSVLPAYSYRWRPDEFNQTVRPAEAIQRLNGMIRK
                     YALEHDCAYVDFWTPMSDDKGGMKDGLSSDGVHPHPDAYFFMEELITPVIQQLCMQ"
     gene            complement(2607..3185)
                     /locus_tag="PLM68_07560"
     CDS             complement(2607..3185)
                     /locus_tag="PLM68_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT55250.1"
                     /translation="MMKSLKGTKTEKNLLAAFAGESQARNRYTYFASAAKKEGFEQIA
                     GVFEETANQEKEHAKRFFKFLEGGMVEITAMYPAGEIGTTAQNLLAAAEGENEEHSLL
                     YPEFAKVAAEEGFPKIAAAFLAIATVEAEHEARYRKLLDRVEKGKYFERDEEIVWQCR
                     NCGYITKGKKAPEICPACQHAKAYFEEKKENY"
     gene            complement(3219..3290)
                     /locus_tag="PLM68_07565"
     tRNA            complement(3219..3290)
                     /locus_tag="PLM68_07565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3255..3257),aa:Arg,seq:cct)
     gene            complement(3308..3490)
                     /gene="rpmF"
                     /locus_tag="PLM68_07570"
     CDS             complement(3308..3490)
                     /gene="rpmF"
                     /locus_tag="PLM68_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT55251.1"
                     /translation="MAHPKHKTSKQRRDKRRTHDKLKSPTLATCSNCGAAVMYHRVCP
                     ECGYYRGRQIIQKTVV"
     gene            complement(3530..4003)
                     /locus_tag="PLM68_07575"
     CDS             complement(3530..4003)
                     /locus_tag="PLM68_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55252.1"
                     /translation="MEQMENTRLRVAVKTLSKGTYDYAFRLNRDFFSSFENADILDAA
                     LDTEIKICKTDEQESIITVKITGTLVVLCDRCLDKLTVPVSFESTLEEEEILECLNDF
                     TGQMDFTQYVYDNICLAIPIKRVHESEKDCDQEMLRIWKQASVQEKNRITNLLPD"
     gene            4096..>4696
                     /gene="guaA"
                     /locus_tag="PLM68_07580"
     CDS             4096..>4696
                     /gene="guaA"
                     /locus_tag="PLM68_07580"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT55253.1"
                     /translation="MNEKILIIDFGSQLTQLIGRRLRELKVYCEIFPYHHFPELDPSV
                     KGMILSGSPYSVKDKDAPVIDLSGIKGKIPLLGICFGAQYLATRYGGDVHSSLSREYG
                     RAMLDILEPGSPLFRNVPSPTAVWMSHQDSIDALPVGAVNLASTDNVQYASFRFEGED
                     TYGVQFHPEVYHSVHGRNILENFALGICQCKGDWTPSGFI"
BASE COUNT         1259 a         1077 c         1041 g         1319 t
ORIGIN      
        1 ggtgatcatg ccaaaactcc tggaagcact gggggttacc tgctacaaga tccattctga
       61 gcctactggt gattttgcac acaatccaga gcccctgcca catcatctga aggacctgtc
      121 atcagaagtg gttgccagaa aagccacact gggcatatcg gtagatccgg atgtggacag
      181 gctggccctt gtatgccagg acggaagcat gtttggcgaa gaatacaccc tggtggcctg
      241 tgcagattat gtgctgggaa aatatgctgc acaatatccg gataaaaaat tacatacggt
      301 atccaatctt tcctctaccc gtgctctgaa agacattacc caaagataca acggttcctg
      361 ttctgcagca gctgtaggag aagtgaatgt ggtggaggaa atgaaacgca ccggcagtgt
      421 gataggcgga gaaggcaacg gaggagtcat cctgccttcg cttcactatg gccgggacgc
      481 acttatcggg accgccctgg tcctctctct gttggcagaa aaaggacaat ccatgaccgg
      541 gctcagaagc tcctacccgg actaccatat gagtaaaaac cgcgttgatc tgaaagaaga
      601 tatgagcctg gaagatctgt cagaccgcat aataaaagca tatcctacag caacagtcaa
      661 caatagggac ggattgcgtc tggattttga aaatgaaaag aagtgggtta gtcttcgtaa
      721 atcaaacacc gaacctatcc tgcgcatata cagtgaggct cccgataaaa ccggcgcaga
      781 atcacttgcc tctgaaatca tgtctttgct ctcattataa tttggttgag ccaaaaaatt
      841 acgtaccttt gcagcccgtt ttacaggaaa ttataaaaaa tacataagat gaaacaagga
      901 attcacccgg aaacctacag gcttgttgct ttcaaagata tgtccaatga ctatgtgttc
      961 gtaacacgct cctgtgtaaa tgccaaagaa accatcacac tggaaggcgt cgaataccca
     1021 ttgtacaagc ttgagatctc caacacctcc catcctttct atacaggaaa gatgaagctt
     1081 gtagacactg caggccgtgt agacaaattc agaagccgtt atggcgatcg taaaaaataa
     1141 cacagcacag catttcacaa aaagctgtcc ccgcaaggac ggcttttttg atgatatatc
     1201 cttacagccg gtgcctactg aggatggttc aaacaccctc atgggcccgt ggggtgagac
     1261 gtatcactcc atttcaggag ccatacagga atcccaacac gtttatatta tcagtggact
     1321 tctttactat ctcaattcac aagtgtccga aggaaggttg ccccatgcga ttcacatact
     1381 ggaagtgggt tttgggacag ggctcaatgc cctcttgaca taccgggcaa tgaattcgtg
     1441 tatatttccc aaagccactc ctgtttatta tgaaacagta gaaatgttcc ctttgccgga
     1501 aaatattacc aatgaactct tatatccgga ttcgccccgg gaaaaggaat ttttcagaaa
     1561 tatccaccgg ggcaagtggg gtgaaccttt tcaggcagca gaaggtttca ccatgctcaa
     1621 acagaaggga aatatacttg actatcaacc gcaggaaggt attcacgttg tctattatga
     1681 tgcattttca cctggggtgc aacctgaatt atggagccgg aatgtatttg ccggaataac
     1741 tccatctctt gaaaaagcag cagtccttac aacctactgt tgcaggggaa tggtaaaaaa
     1801 tgctttacgt gaactgggtt tcttaattga gaggcttccc ggcacaggaa gaaagcgtca
     1861 tatcctgagg gctgtttatt gcatgcaaag ctgttgaatg acaggagtaa tcaattcctc
     1921 cataaaaaaa taggcatcgg gatgaggatg aaccccatcg gacgagagtc cgtctttcat
     1981 gccacctttg tcatcggaca tgggagtcca gaaatccacg tatgcacaat catgctcaag
     2041 ggcatacttg cgtatcatac cgttgagtct ctgtatagcc tccgcgggac ggactgtctg
     2101 gttgaattcg tcagggcgcc atctgtatga ataagccgga agaacagaac agagaataac
     2161 ccgtatattg ttaaaatgtg ccaaatcaac catagacgcg atattatcca tgatctggcg
     2221 gtgtgtcaca tatccctggt tctgtgctat atcattggta cctgccagaa taaccaacac
     2281 tttgggattc aaacttatga catctctcct gaaacgggcc agcatttgta aggtgacttc
     2341 tcctcctatg ccttttccaa caaaattgtt ccttgtaaag aaatccgggt gaaaattcct
     2401 ccagttctgg gttatggaat ttcccatgaa aacagcatca ggcctgatcg gttgttcggt
     2461 atgttcctgt gcatgtgtga taaaaaaaca ggagatgaat aatgatagta caaacaggtg
     2521 tttcatagat ttattttaca gggatattct accggtacaa cgacggccgc atgtccctat
     2581 cagtgaattc gctgcctggt aggttattag taattctctt ttttctcttc aaagtaagct
     2641 ttggcatgct ggcaggcagg acaaatttct ggagctttct tccctttagt gatgtagcca
     2701 caattacggc actgccatac tatctcctca tccctttcaa aatacttgcc tttctctacc
     2761 ctatccagca acttgcggta acgggcctcg tgctctgctt ccactgtagc tatagcaaga
     2821 aaagcagcgg cgattttggg gaaaccttct tccgcggcta cttttgcaaa ttcgggatac
     2881 aacagggaat gttcctcatt ttctccttca gcggcagcca aaagattctg ggctgttgtg
     2941 ccaatctctc cggcaggata cattgctgtg atttctacca tacccccttc aaggaatttg
     3001 aagaaacgtt tggcgtgttc cttttcctgg ttggcagttt cttcaaatac accggcaatt
     3061 tgttcgaaac cttccttttt ggctgctgag gcaaaataag tataacgatt gcgtgcctgg
     3121 gattctcccg cgaaagctgc caggagattt ttttctgtct tagttccttt taatgatttc
     3181 ataatgctag gattaaaaat gggactttat gattattgta gccccaccag gactcgaacc
     3241 tggatttgaa gtttaggaaa cttccgttct atcccttgaa ctatagggcc aatcttttca
     3301 ggtatgatca gacaacagtc ttctgaatga tctgacgtcc gcggtaatac ccgcattcgg
     3361 gacataccct gtgatacatg acggcggctc cgcagttgga acaggttgcc agggtgggac
     3421 ttttcaactt atcgtgtgtt ctgcgcttgt ctcttctctg ttttgaggtt ttgtgttttg
     3481 gatgtgccat atctcgttta cttaaaaaat tattatttca tcaaatccgt caatctggta
     3541 aaagattcgt tatcctgttt ttttcctgaa cagatgcctg tttccaaatg cggagcattt
     3601 cctggtcaca gtctttttct gactcatgta cacgcttgat tggaatggca agacaaatgt
     3661 tgtcgtatac atattgtgtg aaatccatct gaccggtaaa atcattcagg cattcaagga
     3721 tctcctcttc ctctaatgtg ctttcgaaag aaaccggaac agtaagtttg tcaaggcatc
     3781 tgtcacacag aacgaccaga gttcctgtaa tcttaacagt tataatggat tcctgttcgt
     3841 ctgtcttgca tatttttatt tccgtatcca gagcggcgtc cagtatatct gcgttttcaa
     3901 acgacgagaa aaaatccctg ttcaaccgga aagcatagtc atatgtccct tttgaaagtg
     3961 tctttaccgc aaccctcagt cttgtgtttt ccatctgttc catatggccg gaaatgagcc
     4021 gacaaagata aacaaatatt ttagataatc gttgttttgt tttacttttg ctactctata
     4081 actacgggac ttcctatgaa tgaaaaaatc ctgataattg actttggttc acaacttacg
     4141 caattgatcg gtagacggtt acgggaactg aaggtatact gtgaaatatt cccttatcat
     4201 cattttcccg aacttgatcc ttctgtcaaa ggtatgatcc tgtcagggag tccgtattct
     4261 gtgaaagaca aggatgctcc tgttattgat ctttcaggga ttaaaggaaa gatcccgctc
     4321 ctgggcatct gctttggggc acagtacctg gccacccggt atggaggaga cgttcattct
     4381 tcgctgtcac gtgaatacgg aagggccatg ctggatatcc tggaacccgg gtcccctttg
     4441 ttccgtaacg ttccctcccc tactgccgta tggatgtcac accaggacag cattgatgcc
     4501 cttcctgtag gagccgtgaa cctggcctct acagacaatg tgcaatatgc gtctttccgt
     4561 tttgaagggg aagacactta tggtgtacag tttcatcccg aagtgtatca ctcggtccat
     4621 ggccggaata tcctggagaa tttcgcatta ggtatctgcc aatgcaaagg ggactggacc
     4681 ccttccggct ttatag
//
LOCUS       DAOSOF010000142         4517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_19290_length_4517_cov_5.213133, whole genome shotgun sequence.
ACCESSION   DAOSOF010000142 DAOSOF010000000
VERSION     DAOSOF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19290_length_4517_cov_5.213133"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLM68_07585"
     CDS             complement(<1..616)
                     /locus_tag="PLM68_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55254.1"
                     /translation="MFFKLHIVRAALGLFTAILLCSSCAALTESQLQTVQKLAVSGDS
                     VAAAPSAMFRQLAAVRTERGLFYAASLTGAQARFAELNALATAAREDEKLAARTDVCV
                     DVLNRYLRALRSISNPARWKQIGTEMRGLGKNLDSVILRFNQLDWVDLELTQGVAKLS
                     GKYAGYLSENYMKNRQTVAVREFVTEGDTLVAVCVDALVQLLRKQ"
     gene            complement(740..1990)
                     /locus_tag="PLM68_07590"
     CDS             complement(740..1990)
                     /locus_tag="PLM68_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT55255.1"
                     /translation="MKARHLSTYISNRASGKSGRILVFTGARQTGKTTLARHLFPDYE
                     YITIEDPVTVGAFLKLTAAQWQSMYPQAILDEVQKEPVLIESIKAVYDQWQEPRYVLL
                     GSSQLLLLSKVRESLAGRCVIVEMYPLTLPELKTNSIKEKVAYSLFQNFITDPERTPN
                     ILPSFLLDKEMAEKQQAWNHYLQFGGYPALTDTILSDEERYIWLQQYVRTYLERDVRD
                     LASFRDLEPFVKLQRALAAQTGSVINASSLANHTGVTVKTAQRYIHYLEVSYQTITLP
                     AWSRNETKRLMKAPKVHYLDNGVLQAVLQKRGGITGTEFESLVVAELYKQTNVLQMPA
                     KFHHLRIQGGREIDLLLELGKGYIAFEIKMTDKVRKNDARHFAGLDEILDKPLIHCFI
                     LSNDLETQYFTSKVTAVHCALFLG"
     gene            complement(2085..4037)
                     /locus_tag="PLM68_07595"
     CDS             complement(2085..4037)
                     /locus_tag="PLM68_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOT55256.1"
                     /translation="MEQQEKKKGFRKLEERLLNQLGFYRSNYLKYWVIAAIDVVIATF
                     CSVFVFLIYAPIEGPFSRRNVLIVIVLAVIINMISFYLFKTYRNIIRYTTVKGLLPLG
                     YSVAVKSVILTVVLYFAGLLSPGIELRFLLIDLLFTFVALVSIRVLMIIAYDMVNEKF
                     NINSQRVLIYGIDEKAASILIRLHKSKHYKPIGFLVHEHGKRAYRLNEMKVYHFQTRE
                     DVVKLVHKLGAKAILFPSYRGIHAEKNRLIHYCNLEKINIIMVPPIDELGSKDTIQLN
                     IRPIAIEDILGREEITINMDVVSERFRDKTIFVSGAAGSIGSEFCRQVAGLNVVKQLV
                     LLDMAETPMHELRLELEEHFPNLNFVHFIGDVRHKATIHDLLATYKPQIVFHAAAYKH
                     VPLMEENPCEAVRVNVEGTRIMADAAVAHGVEKFIFLSSDKAVNPTNVMGATKRLAEM
                     YVQSLGLAISHGKVEGTTQFVTTRFGNVLGSNGSVIPRFRQQIKDGGPLTLTHKDITR
                     FFMSISEACRLIMKAATISTNNEIMVFDMGEPVLIKDMAERMIHLAGFTPYDEIDIRE
                     VGLRPGEKLYEEVLSTQENSAPTSHPKIRVAAVRANNYQELRDALQQISRRAWERDVP
                     ATVQLLKKWVPEFKSHNSQFEKYDVD"
     gene            complement(4048..>4517)
                     /locus_tag="PLM68_07600"
     CDS             complement(4048..>4517)
                     /locus_tag="PLM68_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55257.1"
                     /translation="NRSAGKEAENHRVALAATENNHPAGKTADRHLPADKGAGRSWIL
                     PLPKALERLAALAMYSDMVDAQRYENQLYRILEHYARDPQIARQVRFLVIVSHMGYFK
                     KYHKLMEQITDHVMQGDLSMEQDKTQQCREQDLIQAVRFYVLAIYYHYRSPRT"
BASE COUNT         1105 a         1098 c         1070 g         1244 t
ORIGIN      
        1 cctgtttgcg cagcagctgt accagcgcat ccacacaaac agccaccagt gtatcgcctt
       61 cggtcacaaa ttcacggacg gccacggtct gccgattttt catgtagttc tcactcaggt
      121 agccggcgta ttttccggac aatttggcca caccctgggt caattccaaa tctacccagt
      181 ccagttggtt aaagcgcagg atcaccgaat ccaggttttt tcccagtccg cgcatctcgg
      241 tccctatctg tttccatcgg gcggggttgc tgatgctgcg cagggcgcgc aaatatctgt
      301 tcagcacatc tacgcacaca tcggtgcggg cggccagctt ttcgtcttcc cgggccgcgg
      361 ttgccagggc gttcaactcg gcaaaacgcg cctgggcccc ggtcagcgag gctgcgtaaa
      421 aaaggccgcg ttcggtacgg acggcggcca attggcgaaa catagcacta ggtgccgccg
      481 ccacactatc cccgctcacc gccagcttct gcaccgtctg cagctgcgat tccgtcagtg
      541 ccgcacaact gctacataat aatattgcag taaacaatcc taaagcagct cttactatgt
      601 gtaatttaaa aaacatctaa actatttgta attataatgg attgttgcac aattgataag
      661 gggaagctcg tcccgcggag ggagggaccc gtttgcaaag caaatgggag gccttccccg
      721 ttcaattcgt gcacaatcct tatcccaaaa acaaagcaca atgaactgct gtaactttag
      781 aagtaaaata ttgggtttcc agatcgttgg ataaaataaa gcaatgaatc aggggcttat
      841 ccaaaatttc atccaacccg gcaaagtgcc gggcatcatt ttttctaacc ttatcagtca
      901 tttttatttc aaaggcaata tatccttttc ccaattccaa tagcaggtct atctccctgc
      961 caccctggat cctcaaatga tgaaatttgg caggcatctg caacacattc gtttgtttat
     1021 acaattcggc aacaaccaga ctttcaaatt ccgtcccggt gatccctcca cgtttttgta
     1081 gaacagcctg caacacgcca ttgtccagat aatgcacttt gggcgctttc attaggcgct
     1141 ttgtctcatt tctgctccag gcgggtagcg taatggtctg gtaactgacc tcaaggtagt
     1201 gaatataacg ctgagccgtc ttaacagtta cgccggtgtg gtttgccagg gaagaggcat
     1261 tgatcacgct ccctgtctgc gcagcaagtg cacgctgtaa tttgacaaac ggttccaggt
     1321 ccctgaatga ggcaagatcc ctaacgtctc tttcaagata agttcgtaca tattgttgta
     1381 accatatata acgttcttcg tccgaaagaa tcgtatctgt taatgcggga taaccgccaa
     1441 attgcaagta atgattccat gcttgttgtt tttctgccat ttccttgtca agaagaaaag
     1501 agggaagtat attaggtgta cgttccggat ctgtaatgaa attctgaaac agggaatatg
     1561 ctactttttc ctttatacta ttggttttca gttcaggaag tgtaagcgga tacatttcta
     1621 caatcacaca acgtcccgca aggctttccc tgactttgga taaaagcaac aactggcttg
     1681 atcccagtaa gacataacgg ggttcctgcc attgatcata tactgcctta atactttcaa
     1741 ttaaaaccgg ttctttttgt acttcatcca aaatggcctg tggatacatg ctctgccatt
     1801 gggcagctgt cagtttcaaa aacgcaccaa ccgtgacagg atcttcaatt gttatgtatt
     1861 cgtaatccgg aaacagatgc cttgccaatg tggttttacc cgtttgacgc gcaccggtaa
     1921 acacaagaat acggcctgat ttaccggagg ctctatttga aatgtaagtt gacaggtgtc
     1981 gggccttcat gcttttgact ttttattcca aatataactg ttttttctgg attttcattc
     2041 caaatatcac tgtattttct ggactatcac acttagggac tgcatcagtc aacatcatac
     2101 ttttcaaatt gagagttatg gcttttgaat tccggcaccc attttttgag cagctgcacc
     2161 gtggctggca catcccgctc ccacgcccgg cggctgattt gctgcaaggc atctcttaat
     2221 tcctggtaat tgttagcacg tacggcagcc acacggatct tgggatgcga cgtaggagca
     2281 gaattttcct gggtgctgag cacctcttcg tatagttttt ctccgggccg aaggcccact
     2341 tccctgatat caatttcatc atacggcgta aacccggcca ggtggatcat gcgctcggcc
     2401 atgtctttaa taagtaccgg ttcgcccatg tcaaagacca tgatttcgtt gttggtgctg
     2461 atggtagccg ctttcatgat caggcggcag gcttcggaga tgctcatgaa aaaccgggtg
     2521 atgtctttgt gtgtgagggt gagcggtccg ccgtccttga tttgctggcg gaaccgagga
     2581 atgacagaac cgttggaacc caggacgttc ccaaagcggg tggtcacaaa ttgggtggtt
     2641 ccctccacct ttccgtgtga aatggccagg ccgaggctct ggacatacat ctcggcaagt
     2701 cgtttggtgg ctcccatcac gttggtggga ttgaccgctt tgtccgagga aaggaaaatg
     2761 aacttttcca ccccgtgagc cacggcggca tcggccatga tgcgcgtgcc ttctacgttc
     2821 acgcgaacgg cttcgcaggg attttcttcc atgagcggta cgtgtttgta ggcggccgca
     2881 tggaaaacga tctggggttt gtaggtggcc agcagatcat gtatggtggc tttgtggcgg
     2941 acatcgccaa tgaagtgaac aaagttcaga ttgggaaagt gttcttccag ttccaggcgc
     3001 agttcgtgca tgggcgtctc ggccatatcc agcaggacca gttgtttgac cacattcagg
     3061 ccggctacct ggcggcagaa ctcgctgcca atagagccgg cagccccgga aacaaagatt
     3121 gttttgtcgc ggaaacgttc acttaccaca tccatattga tggtgatctc ttcgcggccc
     3181 agaatgtctt caatggcaat tgggcgtatg ttgagctgga tggtatcctt gctacccagt
     3241 tcatctatgg gcggaaccat tatgatgttg atcttttcca ggttgcaata atgtatgagc
     3301 cggtttttct ctgcatggat tccacgatag ctggggaaca agatggcttt tgcacccagt
     3361 ttgtgaacca attttacaac gtcttcccgt gtttggaaat ggtagacctt catttcgtta
     3421 agccggtatg ctctcttgcc gtgttcatgt accaggaagc caatgggctt gtaatgcttg
     3481 ctcttgtgca ggcggatgag aatagaggct gccttttcat caatcccgta gatcaggacc
     3541 cgctgggaat tgatattgaa tttttcgttc accatgtcat aggcaatgat catcagaaca
     3601 cgtatggata ccagggctac aaaagtgaat agcagatcta taaggaggaa tctgagttct
     3661 attcccggtg aaagtaagcc tgcaaaataa agtacaacag taaggataac agattttacg
     3721 gctaccgagt aacccagtgg gagcaaccct tttacggttg tgtaccggat gatgttgcgg
     3781 tatgttttga acaggtaaaa cgatatcatg tttatgataa ccgccaaaac aatgacaata
     3841 agcacgttac gtcttgaaaa cggcccttct atgggagcat aaattaaaaa gacaaaaacc
     3901 gaacagaacg tagctattac cacatcaata gctgcaatta cccaatactt caggtaattg
     3961 gaccgataga atcccagttg attcaataac cgttcctcta acttccggaa acctttcttt
     4021 ttttcttgct gttccataaa acaatgttta agttcttggg gagcggtaat gataataaat
     4081 cgccagtaca taaaaccgga cggcctgtat aaggtcctgc tcccggcact gttgtgtttt
     4141 gtcttgttcc atagagagat cgccctgcat gacatggtct gtgatctgtt ccatcagttt
     4201 gtgatacttt ttaaaatatc ccatgtggct cacaatgacc aaaaaacgta cctgcctggc
     4261 tatttgcgga tcacgcgcat aatgctccag aatcctgtaa agctgatttt cataacgttg
     4321 cgcatctacc atatctgaat acatcgccaa ggctgcaagt cgctccagtg cctttggaag
     4381 gggaaggatc cagctgcgcc ctgcgccctt gtctgcaggt aggtgcctat ctgcagtttt
     4441 gccggcggga tggttgttct ccgtagctgc gagggcaacg cggtggttct ctgcttcctt
     4501 gccggcggac cggttgt
//
LOCUS       DAOSOF010000143         4477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_19502_length_4477_cov_3.770918, whole genome shotgun sequence.
ACCESSION   DAOSOF010000143 DAOSOF010000000
VERSION     DAOSOF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19502_length_4477_cov_3.770918"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..3486
                     /locus_tag="PLM68_07605"
     CDS             340..3486
                     /locus_tag="PLM68_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT55258.1"
                     /translation="MIAGPVRAQNVNVTGKVSDNNGAPLMGVYVLVQGTSRGVSTDLD
                     GMYSINAPADGTLLFSFMGFDNVAEQINRRSIIDVTMTVSSVALEELVVTALGIKKDR
                     KALGFSVTEVGAEELLKNKQTNVINSLAGKVAGVNVTQSGGSAGSGSTIIIRGGNSAS
                     EGRDNQPLFVVDGIIYDNSTVIGGNSGTDGMTKTASTFGNRVMDINPEDIESMSVLKG
                     AAAAALYGSRAADGVIVITTKKGATDGTVKVNVGSKYSYSWANSLPELQGVYGRGYYN
                     QAGVFSDYTYNSWGDKISGKVYDNVGNFFRGGNIWDNSVSVSGGSKNGSFYLSASRYD
                     QQGIVPMTGYDKTTLRFNGEQKYGKLTVGANVAYSISNTRKTLTTGGLYGGGGNGAMT
                     AVYGWSRSDDMTYYLNEDGTKYRMFPQYQDLASDLENPYWIINKNKLSDQTSRWTGSV
                     NASLDLTDWFNVVYRVGLDTYTYDEYTYIAPGGAVSERYQNGRLSKSDRTYQYLSSNL
                     MLNFHKTFGDFDFNLLLGQTFEDTKNIRQNHWGYDFVTAGTISFSNIANENKFFTEST
                     TLKRLIGVYGEFRAAYKNIAYLTVTGRNDWSSTLPVENRSYFYPSVSGSLVFTELLPK
                     NDILSFGKIRASWAQVGKDASAYATNTYMWSPAAVSPGFIGIGNNWTRGNPFLKPEIQ
                     TSWEVGAELRFFGGRLGIDYTYYNSTTTNQIAAPRLGQSTGYIFMSINSGSVSNKGME
                     LMITATPVETKNFSWDLTLNLSGNRGTLGEFMKGVDLFYVTDVQIGGVKAAAVPNGGY
                     FLGITGDYWKRETLKDGDGNVVKDDNGKEVENPDGRYIIDETTGLYKTTGVATNVLGN
                     REPKFIGGFGNTLTYKNLSFSILLDIRKGGLIYNGTEYLLLNNGLSPQTLERESVTVE
                     GVSSVTGEPVSYTYKAGQTYIVNKAEKSGEYMIQSYWSNYATNAYNLLTDTNWLRLRA
                     ISLSYDFKGLFSKQSFIKGLTATVTGTNLFLWSNYKGMDPEVSVSGSGTGGSGSSGID
                     YCGVPATAGLSFGLNISF"
     gene            3514..>4477
                     /locus_tag="PLM68_07610"
     CDS             3514..>4477
                     /locus_tag="PLM68_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOT55259.1"
                     /translation="MKKILYIALSLLLILGISGCNEWLDVNVDPDNPNNSSATVDSRL
                     PWIQHYYMYAWGTASMRGVTIGGLMTQTSTTSANGKLSAWDPLQSSTTTSYQNWFIGA
                     ACNIDDMIAAAEKEGAYHYIAAAHAIHAMGYMLMTDLYGEMPYSEPLGSNPTPVYDDG
                     QTIFNGCMERLDLAIEFFNKTQEPGATPLAAGDSWNNGDVQKWLKLCYGLKARWLNKL
                     SKKSALYNPQAVLDALAKAPQSNNDNTIMKHSNAGDQDTNFTVGDPYQTSVIWDCSAW
                     GATQRYTKWYVDLHTNLKNSGVTDPRLAKVFPARMSNIKINDAGA"
BASE COUNT         1239 a         1111 c         1065 g         1062 t
ORIGIN      
        1 agattggggg aaaaaggtaa aagttacaac ggataaaaat ccgtgaagta gaaatcattc
       61 aggttaaagg tttaaatttc atcaaaaaaa agaacaaggc gggagacggg gcacggggaa
      121 aagaacgaag aggaaagatt cattcatctt tgcgaatata ggaaataatc ttacaacaag
      181 gaacaatagt gatttttttt agttacattt gttgactgtt acctgtaata agaaaacaaa
      241 ccgaaacctt attatcataa cctaaaataa ttacttatgg atgtttacaa aaaaagcggt
      301 cgcctgactc tattttgctt ggtgaccttg gttgctgcaa tgattgcagg accagtgcgg
      361 gctcaaaacg taaatgttac cgggaaggtg tccgacaata acggagcacc cctgatgggc
      421 gtttatgtgt tggtccaggg tacatcacgg ggcgtctcca cagaccttga cggtatgtat
      481 tccatcaatg cgcctgcaga cggaaccctt ctattctcat tcatgggatt tgacaatgtg
      541 gctgaacaaa tcaacaggcg ttccatcata gatgtaacca tgactgtatc ttccgtcgca
      601 ttggaagagc ttgtggttac cgccctcggg atcaagaaag acagaaaggc cctcggattc
      661 tctgttaccg aagtgggcgc cgaggaattg ttgaaaaaca aacagaccaa cgttattaat
      721 tcccttgccg gtaaagtggc cggtgtcaac gttacccaat ccggtggctc ggccggttcc
      781 ggttctacca ttatcatacg agggggcaac tcggccagtg agggtcgtga caaccagccc
      841 ctctttgtgg ttgacggtat catatatgat aactccaccg tcattggcgg taattcaggt
      901 acggacggta tgaccaaaac tgcctccact ttcggtaacc gtgtcatgga catcaaccct
      961 gaggacatcg agtccatgtc tgtgctgaaa ggcgcagcag ctgccgcctt gtacggttca
     1021 cgcgcagcag acggggtaat tgtcatcact acgaagaaag gagcaacaga cggaactgtc
     1081 aaggtaaatg tgggctcaaa atacagctac tcatgggcca attcactgcc tgaacttcaa
     1141 ggggtttacg gaaggggcta ttacaaccag gccggtgttt tcagcgatta tacgtacaat
     1201 tcctggggcg ataagatttc cggaaaggta tacgacaacg tgggtaattt cttccgtgga
     1261 ggaaacatct gggacaacag tgtcagtgtt tctggtggaa gcaagaacgg cagtttctac
     1321 ctgtcagcct cacgttatga tcaacagggt attgttccca tgaccgggta tgacaaaacc
     1381 accctgcgtt tcaatggtga acagaaatat ggaaaactga ccgtgggcgc caatgtggcc
     1441 tattccattt ccaacaccag gaagaccctg accactggcg gtctttacgg aggcggtggc
     1501 aatggtgcca tgacggccgt atacggatgg tcacgcagtg acgacatgac ctattacctg
     1561 aacgaagatg gaaccaaata ccgtatgttc ccccaatatc aggacctggc atctgacctt
     1621 gaaaatcctt actggataat caataagaat aaattgtccg atcaaaccag ccgctggacg
     1681 ggatcagtga acgccagcct ggacctgacc gattggttca atgttgtata ccgtgttggt
     1741 cttgacacgt atacatatga tgaatacacc tacatagctc ccgggggggc tgtctctgaa
     1801 agataccaga acgggcgtct ctccaaaagc gaccgtacct accaatacct gtcctcgaac
     1861 ctgatgctga atttccacaa aacattcggt gactttgatt tcaacctgct tttgggacaa
     1921 acttttgaag acacgaaaaa tatcaggcaa aaccactggg gttacgattt cgtgacagca
     1981 ggtaccatca gcttttccaa tatcgccaat gaaaacaaat tcttcacgga atcaaccacc
     2041 ctcaaacgtc tgatcggggt gtatggcgag tttcgcgctg cctataagaa tattgcttac
     2101 ctgacggtta ccggacgtaa tgactggtct tccacacttc ctgtcgaaaa ccgttcctat
     2161 ttttatccgt cagttagcgg tagcctggtt ttcaccgagc tgctacccaa gaatgacatc
     2221 ctgtcctttg gaaagatccg tgcctcatgg gcacaggtgg gtaaagacgc gtccgcttat
     2281 gcgaccaaca cctatatgtg gtcaccggct gctgtaagcc ctggatttat aggtatcggc
     2341 aacaactgga cacgcggtaa tcccttcctc aaaccggaaa tccagacatc ctgggaagtg
     2401 ggtgccgaac tccgcttctt cggaggacgc ctgggtatag attacactta ttacaatagt
     2461 accacaacca accagatcgc agctccccgc ctggggcagt ccaccggtta tattttcatg
     2521 tccatcaact cgggttctgt atccaataag ggcatggagc tgatgatcac cgcaaccccc
     2581 gttgaaacca agaacttcag ctgggacctg accctgaacc tttcaggaaa ccggggcacc
     2641 ctgggtgagt tcatgaaggg agtggacctg ttctatgtca ccgacgttca gattggcggc
     2701 gtaaaagcag cagccgttcc caacggagga tatttcctgg gtatcaccgg ggactactgg
     2761 aaacgtgaaa cgctaaaaga tggagatggc aacgtggtaa aggatgataa cgggaaagag
     2821 gttgaaaatc ctgatggccg ttatattatt gacgaaacca ccggtcttta caaaaccaca
     2881 ggtgtggcca ccaacgtgct cggcaaccgt gaaccaaaat tcattggagg atttgggaac
     2941 accttgacct ataagaacct gagcttctcc attctcctgg atatccgcaa aggcggtctg
     3001 atctacaacg gaactgaata cctgctgctc aacaacggtt tgtcaccaca gactttggag
     3061 cgcgaatccg tcaccgttga aggtgtatcc agcgtcacag gcgaacctgt ctcctataca
     3121 tacaaggccg gacagaccta catcgtgaac aaggccgaaa agagcggtga gtatatgatc
     3181 cagagctact ggagcaatta tgccaccaat gcctacaacc tgctcacaga taccaactgg
     3241 ctgcgtttgc gtgccatatc tctctcttat gatttcaaag gcttgtttag caaacaaagt
     3301 ttcatcaaag gtcttaccgc cacagtaacg ggaacaaacc tcttcctgtg gagcaattat
     3361 aaaggaatgg atcctgaagt cagcgtttcc ggctcgggaa caggcggatc cggatcgtcc
     3421 ggtattgact attgcggtgt tcctgctaca gcaggcttgt catttggtct aaatatttct
     3481 ttctaagaac taacggattt aaaaactcag attatgaaaa aaatacttta tatagcttta
     3541 agtctgttgc ttatcctggg aatctccggt tgtaatgaat ggctggatgt gaacgtggac
     3601 cctgataatc ccaacaactc cagcgctacg gtggatagcc gtcttccctg gatccagcac
     3661 tattacatgt atgcatgggg tactgcctcc atgcgcggtg taaccatagg aggtctgatg
     3721 acccagacat ccactacttc tgccaacgga aaactctctg catgggatcc cctgcaatct
     3781 tccactacaa catcctatca gaactggttt atcggcgcag cctgcaatat tgacgacatg
     3841 atagctgccg ctgaaaaaga aggagcctat cattacatag ctgccgccca tgcgattcat
     3901 gccatgggtt acatgctcat gaccgatctc tacggcgaaa tgccttattc cgaacccctg
     3961 ggcagtaatc ccactcctgt ttatgatgat ggccagacca ttttcaacgg ctgcatggag
     4021 cgccttgacc tggccattga gtttttcaac aagacccagg aacccggcgc tacaccactc
     4081 gctgccggtg acagctggaa caacggtgat gtgcagaaat ggctgaaatt atgctacggg
     4141 ctcaaagccc gatggctgaa caagctttcc aagaaatctg ccctgtacaa tccccaggca
     4201 gtactggatg ccctggccaa agccccgcaa tccaataacg acaacaccat catgaaacac
     4261 agcaatgcag gtgaccagga taccaacttc actgtgggtg acccctacca gaccagtgtt
     4321 atatgggatt gttcagcctg gggcgccaca caacgttata ccaaatggta tgttgacctt
     4381 cacaccaacc tcaaaaattc cggcgtaact gatccccggc tggccaaggt gttccctgcc
     4441 cgtatgagca acataaaaat caatgacgca ggagcca
//
LOCUS       DAOSOF010000144         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_19581_length_4462_cov_4.572952, whole genome shotgun sequence.
ACCESSION   DAOSOF010000144 DAOSOF010000000
VERSION     DAOSOF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19581_length_4462_cov_4.572952"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLM68_07615"
     CDS             complement(<1..989)
                     /locus_tag="PLM68_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55260.1"
                     /translation="MTKRHASLYIARKLKGDATSVGKVSRMGTRIATISVAISVFTMI
                     LSIAIVRGFRSEIEKRAVGFTGEILMEAPGAGFVTEPIPVSNKLSYLPDLKEHPEVSH
                     IQEFAMSYGIMRTEDAIQAVSFKGVGLSYRWDFFARHLREGRLPAFSDTASSSEVLIS
                     KRLSSMLGIDVDQVITMYFIGNSVRMRRFVVSGIYDAQLEDIDNHLVVGDIRHAQRLN
                     GWREDQVSGMELFLTSKERLDAKQEEIEEFILEKTSDTDDGVVLKNIHRIYSHLYDWL
                     DLMDINVVVLLTLMVLVAGFNMMSGLLILLFEKTATIGLLKAVGMRDRHIRNS"
     gene            1028..1100
                     /locus_tag="PLM68_07620"
     tRNA            1028..1100
                     /locus_tag="PLM68_07620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1061..1063,aa:Gly,seq:ccc)
     gene            complement(1166..2059)
                     /locus_tag="PLM68_07625"
     CDS             complement(1166..2059)
                     /locus_tag="PLM68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOT55261.1"
                     /translation="MVYDKEILYIISLLDDPDQYVQDALFRQLQNIGRPALEQLEGVV
                     RESGEEKYPGTGRMLAALRQELKFNALKEWIDKPGDDLLTGLCLVQDILTGDAGIQDL
                     QEILMDCVNEISVEMRDDQTVMEKVKLLNHVFFHRLCFKTIDPMFSSPGNTFIHHAFQ
                     KREGSPIAIGIIYLLLAYHSGVQIRGRVFKGGFLPAVIDISGNVLFYVNVYKNGLLFP
                     HTELESFLEDLNLQIPPESFREARPEDLAQIYAETLYYSYSMREDNTESEKLNKLEKI
                     LSFFGRESSLLIEIDDDEEDD"
     gene            complement(2063..2956)
                     /locus_tag="PLM68_07630"
     CDS             complement(2063..2956)
                     /locus_tag="PLM68_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOT55262.1"
                     /translation="MKYIEPSELVINSDGSVFHLHMHPDQLADNVLLVGDPDRVKLLR
                     GMLTQIESDTRSREFVSVTGTYKNKRITLLSTGIGTDNIDIVMNELDALANVDFKTRT
                     IKKEHRTLKILRLGTSGAIQPDIPTGSLVYSAYSAGIDGLLNWYQGRDKVCDLEMEKA
                     FMEHMNWSPTLATPYFVQNSRVIGNSYSNAFSGITLSAPGFYGPQGRVIRLALSDPGY
                     LSKLASFRYNGLRIMNFEMEGSAIAGLSRLLGHEGATVCMIIAQRSNKIMNVDYKEMM
                     YEKAGEALDRLAMEEAAIERL"
     gene            3024..3407
                     /locus_tag="PLM68_07635"
     CDS             3024..3407
                     /locus_tag="PLM68_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HOT55263.1"
                     /translation="MIQRIQTLYLAIALGLMVSILFGIYATYTPFVILTSASCILLLA
                     AILLFKARKMQIRLCIYNSLIMLGLQVWMGAIYIKNMSDIAFQVTTVFPIVSIILLIL
                     AIRAISIDEALVQSYNRLRSKKEKK"
     gene            3470..>4462
                     /locus_tag="PLM68_07640"
     CDS             3470..>4462
                     /locus_tag="PLM68_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT55264.1"
                     /translation="MEKNISRRDFFQKSALATAGAIVATTPLMANIPLDNQKKKKSAP
                     TDKLNIAGIGIGGRGGGVIREMNSENIVALCDVDWKYAGPQLEAYPNAKKYKDFRKMF
                     DEMGSQIDAVVVGTADHTHAVIAARALSMGKHVYCEKPLTHTVYETRLLTRLAAKQKV
                     ATQMGNQGASGNGVRQICEAIWSGMIGEVTRVEAFTDRPIWPQGLETPTEVHTPHPEL
                     DWDLFIGPAPYRPYNQIYHPWNWRGWWDFGTGALGDMACHILQPAVKALNLKSPVAVQ
                     GSSTALMTDCAPNAQIAKFIFPARDNMPKVGMPRVEVTWYDGGLLPEYPKGWPAG"
BASE COUNT         1161 a         1139 c         1000 g         1162 t
ORIGIN      
        1 aaactgttcc tgatatggcg gtccctcata cccactgcct ttagcaatcc aatggtcgcg
       61 gttttttcaa aaagcaggat cagtaatcct gacatcatgt tgaaacccgc caccaggacc
      121 atcagtgtca acagtaccac cacattgatg tccatcagat ccagccagtc gtacaaatgt
      181 gaatagatcc tgtgaatatt cttcaatacc acaccatcgt cggtatcgga agttttctcc
      241 aggataaact cttcaatctc ttcctgtttt gcatccaggc gttctttgct tgtcagaaaa
      301 agctccatac cgctgacctg gtcttcccgc catccattca ggcgctgcgc atgacgtata
      361 tcaccaacaa ccagatgatt atcaatatct tcaagttgcg catcgtatat ccctgacaca
      421 acaaagcgcc tcatcctcac actattgccg ataaaataca tggtgataac ctggtcgaca
      481 tctatcccaa gcatagatga aagacgcttg gaaatcagta cttcactgga cgaggcagta
      541 tccgaaaagg ccggaagacg cccttccctg aggtgcctgg caaagaaatc ccaacggtaa
      601 gaaagtccaa cacccttgaa ggatacggcc tgtatggcat cctctgtcct catgatccca
      661 tatgacattg caaattcttg gatatgtgac acttcaggat gttctttcaa gtccggcaaa
      721 tacgataatt tatttgacac aggtatgggt tcggtcacga atcctgctcc gggagcctcc
      781 attaggatct cgccggtaaa accaaccgcc cttttctcga tttctgagcg aaaaccccgg
      841 acaatggcta tggacagaat catggtaaag acacttatag ccacactgat ggtggctatt
      901 cttgtcccca tgcggctgac ctttcccacc gaggtggcat cgcctttgag ttttctggct
      961 atgtagaggg aggcatgtct cttggtcata aaaaattatt agctatattt gaggattgtt
     1021 tggtaacgcg ggaatagctc agccggtaga gcatcagctt cccaagctga gggtcgcgag
     1081 ttcgagcctc gtttcccgct caaaaccaaa cctgaaacct aacctacacg gagggctgtt
     1141 cttaacagga cagccttttt ttatttcaat catcctcttc atcatcatcg atttcaatca
     1201 gcagggaact ctcccgcccg aagaatgaca gtatcttttc aagtttattg agcttttcgc
     1261 tctccgtatt gtcttcccgc atggaataac tgtaatacag tgtttcggca taaatctgtg
     1321 caagatcttc aggtcttgct tcccggaatg attcgggggg tatctgcagg ttcaggtcct
     1381 ccaggaaaga ttccagctcg gtatgcggaa aaagcaatcc gtttttatag acattcacat
     1441 agaaaagcac attccccgat atatctataa cagccggcag gaaaccgccc ttgaaaacgc
     1501 gtccccttat ctgtacccct gaatggtatg ccagtagcaa atagataatc ccgatggcta
     1561 tggggctgcc ttcccttttt tgaaacgcat ggtgaatgaa tgtattcccg gggctggaaa
     1621 acatgggatc gatcgtttta aaacacaacc tgtgaaagaa gacatggttc agcaacttta
     1681 ccttttccat gactgtctgg tcgtcgcgca tctccacact gatctcgtta acgcaatcca
     1741 tcaggatttc ctgaagatcc tgaataccgg catctccggt cagtatatcc tgtaccaggc
     1801 aaaggccggt tagcaaatca tcgccgggtt tatcaatcca ttctttaagc gcattgaatt
     1861 tcaattcttg cctgagggca gccagcattc ttcctgtgcc gggatatttt tcttctccgg
     1921 attccctgac caccccttcc agctgttcaa gggcaggacg tccgatgttc tgcaactgcc
     1981 tgaacagggc atcctgaacg tactggtcgg gatcgtccag cagtgatatg atgtacaata
     2041 tttccttatc gtataccatc tctcatagtc tctctatggc tgcttcttcc atggctagcc
     2101 tgtctaatgc ttccccagcc ttttcataca tcatttcctt gtaatcgacg ttcatgattt
     2161 tattgcttct ctgggcaatg atcatgcaaa cggtagcccc ctcgtggccc agtaagcggg
     2221 acaatcctgc aatggcagat ccttccattt caaaattcat gatccttaat ccattgtatc
     2281 ggaaagaagc cagtttggac agatatccgg gatcggacag ggcaagcctg atgacacgtc
     2341 cctgtggtcc gtagaatccg ggggccgata gtgtgatgcc gctgaaggcg ttgctgtaac
     2401 tatttcctat cacccgggag ttctgcacaa aataaggagt tgccagtgtg ggtgaccaat
     2461 tcatatgttc catgaatgcc ttttccatct caaggtcaca gaccttatcc cttccctggt
     2521 accaattgag caatccgtcg atccctgccg aatacgctga atacaccagg gagccggttg
     2581 ggatatccgg ttggatggca cctgaagtgc ccagacgcag tattttcagg gtacggtgtt
     2641 ccttctttat ggtcctggtc ttgaaatcaa cattggccag ggcgtccagt tcattcatca
     2701 caatatcaat attgtctgtg cctatcccgg tcgaaagcaa cgtgatcctc ttgttcttat
     2761 aggttccggt gacagaaacg aattccctgc tccgtgtatc tgactcgatt tgtgtaagca
     2821 tcccgcgcaa caatttcaca cggtctggat cgccgacaag cagcacattg tctgcaagtt
     2881 gatcaggatg catatgcagg tggaacacgc tgccatcgct gttaatcacc aattctgacg
     2941 gttcaatgta tttcatgtta caaatttaga ggaattcagc ggattttcct ctacatttgt
     3001 ttcaacaaac acaatcctaa aaaatgatcc aaagaattca gactctatac ctggccatag
     3061 cgctgggact catggtatcc atcctgttcg ggatatatgc aacctacacg ccttttgtca
     3121 tactcacgag tgcatcctgc atactcttgc tggcggctat cctgctattc aaagcgcgca
     3181 agatgcagat acgcctatgc atatacaatt ccctcatcat gctgggcctt caggtctgga
     3241 tgggggcgat ctatatcaaa aacatgtcgg atattgcatt ccaggtcacc actgtgttcc
     3301 ctatcgtaag catcattctt ctcattcttg ccatcagggc catcagcatt gatgaggcgc
     3361 tggtacaatc atacaacagg ctgcggtcaa aaaaggagaa aaagtgattt ttttattatc
     3421 ttcgtaattt gatgtcaacc atttcattta actaataatt caattattta tggaaaaaaa
     3481 catttcaaga agggatttct tccaaaaaag tgccctggcc actgccggtg ccatagttgc
     3541 cacgactcct cttatggcaa atataccact ggataaccaa aaaaagaaga agagcgcacc
     3601 aactgacaaa ctcaatattg ccgggatcgg gattggtgga cgaggaggtg gggtgattcg
     3661 cgaaatgaac tcggaaaata ttgttgcatt gtgcgatgtt gactggaaat atgccggacc
     3721 acagctggag gcctatccaa atgcaaagaa gtacaaggat tttagaaaaa tgttcgatga
     3781 aatggggagc cagatcgatg ccgtagtggt aggtacagca gaccataccc atgcagtgat
     3841 cgctgcccgg gcattgtcca tgggaaagca cgtttattgt gaaaagccac tgacacatac
     3901 tgtttacgag acaagactgc tcaccaggct tgcagccaaa caaaaagtgg ccacccagat
     3961 gggcaatcag ggagcctcag gcaacggtgt tcgccagatc tgcgaagcca tctggtcagg
     4021 aatgatcggg gaagtgacaa gggtagaagc cttcacggac aggccaatct ggccccaagg
     4081 cctggaaacg cccaccgagg tccatacacc gcatcccgaa ctggactggg atcttttcat
     4141 aggaccggca ccctaccgtc cctacaacca gatttaccat ccttggaact ggcgtggctg
     4201 gtgggatttc gggactggag cccttggcga tatggcctgc catatcttac agccggcagt
     4261 aaaagcactg aacctgaaat caccggtagc tgtccaggga agttcaacag ccctgatgac
     4321 cgattgcgcc cccaacgccc agattgcaaa attcattttt ccggcacgcg ataacatgcc
     4381 caaggtgggt atgccccggg tggaagtgac ctggtacgat ggcggactct tgccggaata
     4441 tccgaaagga tggcctgcag ga
//
LOCUS       DAOSOF010000145         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_19780_length_4425_cov_7.025858, whole genome shotgun sequence.
ACCESSION   DAOSOF010000145 DAOSOF010000000
VERSION     DAOSOF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19780_length_4425_cov_7.025858"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(836..1816)
                     /locus_tag="PLM68_07645"
     CDS             complement(836..1816)
                     /locus_tag="PLM68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT55265.1"
                     /translation="MDEKLLIELITKYKNLGIDKQIDYDKFYLYSIITHSTAIEGSTV
                     TEIENQLLFDQGISASGRSMNEQLMNLDLKAAYEKAVVYAKEHRGISVEILKKLAGIL
                     MRNTGSEYNTSLGSFSSAKGDLRLLNVSAGFEGKSYLNYSKIPAALDDFCQWLNDARK
                     TYTDTLSLYKMSFDAHYKLVTIHPWADGNGRMARLLMNMLQFEAGLIPSIIKKENKVK
                     YIESLIQTRESGNLEIFSNFMFEEHISNLKERIANYELSSAEDAPVNVPVKISSRQAS
                     IIDLIRIDSSVTTDDLARKIHVSSKTVKRDIAYLKQQGILLREGSDKKGS"
     gene            2141..>2790
                     /locus_tag="PLM68_07650"
     CDS             2141..>2790
                     /locus_tag="PLM68_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55266.1"
                     /translation="MKQSILLLTVLITAASATAQEPVQVQKKPLDHSVYDAWKSVGAF
                     SMSEDGKYASYLVQEQEGDRYAEVLDLVTLKKQRIERATQPKLTPDGRFLVASINPFF
                     KETKEAKLKKVKKEEMPKDTLGIYNCETGELKKIPFLKSVKIGKLGKTHIAFQCDMPA
                     DTTGGKKPEKIEKDQGQDLMVYHLATGSTDTLPAVSDYNFSTGGDTLFFVRRPHSK"
     assembly_gap    2791..2890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2891..>4425
                     /locus_tag="PLM68_07655"
     CDS             <2891..>4425
                     /locus_tag="PLM68_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT55267.1"
                     /translation="LSWRQLSTDEHHIVQVPQEYDADWGYSVSDYPYQLESSWSSNPR
                     SDLYLINITDGSASALLKDAYISGVSASEEGKYLVWFNNQDQHWYSCDVQSRQIVCMT
                     KGLDVSFANELHDTPEMASSYGNEGWLEGDQAIFINDRYDIWQMDPSGEMAPVNLTDG
                     LGRRENLTFRIVRLDETLLPAGTPGVKKEPFKPKETIYFSVFDNKTKESGYYYKEMGK
                     RRPVMTRWVQEPVTFAYLTRSKDGKVMIYSKQNFVNSPDVWFTKDKFKTQVRITDINP
                     QQKEYNWGTAGLVHWKSATGLDLEGILYKPENFDPAKKYPMIVYFYERTSQTLHTYRA
                     PAPSRSTINIPFFVSNEYIVFVPDIAYQTGHPGKSALDCIVPGVKKLIEENSWIDADN
                     IAIQGQSWGGYQVAYMVTQPQVFKWKAAGAGAPVSNMTSAYGGIRWGSGMVRQFQYEH
                     SQSRIGQNLWDGFDLYIENSPLFFADKVETPLLIMHNDKDEAVPWYQGIELFTALRRL
                     GKPV"
BASE COUNT         1261 a         1002 c          976 g         1086 t
ORIGIN      
        1 acaccgaaaa accgcaaaaa cagggggagg tggtaagtga tggaaaacgc gaacatgcct
       61 ctaatgcaca ggtaaacaat acgttatgcg tttttgtaac atggtcgtta cttaccacct
      121 ccctgcaaaa ccgatgaaat ccagggaggg tggtaagtaa ccaaaaaggc ggagactctc
      181 gtaacatgct atttatttgc gcattacgga ttcttataat ataacagcca cttactacct
      241 ccccccaaaa acaccgaaaa accgcaaaaa cagggggagg tggtaagtga tggaaaacgc
      301 gaacacgtcc ttaatgcgca catatactga ctgttgtgtg tttttgtaac atggttgcta
      361 cttaccacct cccacaagca gccgggaaca atgataagtg ctgcggagca gcgccttaat
      421 atgcgccaaa ggcctttggc gcaccgcctt gaaagaccat taggcggtca attcacgaat
      481 agttacttct cactcctttt atcacagtga ataagtttaa ccgttttata aaaaaggatc
      541 aatgtcaaag ctgaataaac aacaaaaatt aaacaaccaa gccagattgg tggatcaatc
      601 cgacgaaaga tcagaatgag tcccagtaca atagctacag aagtgggaat actgatagaa
      661 gtatctaatt taattttact tttacaattt gggcattccc attttgtaaa ggaccactca
      721 gttataacat gatagaattt ctgtttatga ctacattcgg gacatagaaa tgtttttgtt
      781 ttcatggcac ctgatgaaaa aattaatcaa tggggtcaat gggaacagag ataatctaac
      841 ttcctttttt gtcggatcct tcacgtaata gaatgccttg ttgtttgaga taagcaatat
      901 cacgttttac agttttggaa ctgacatgaa ttttacgagc aaggtcatca gtcgtgacag
      961 atgaatctat ccgaatgaga tctatgatgc ttgcctgtct tgatgaaatc tttacaggga
     1021 catttacagg ggcatcttca gcagatgata attcataatt ggcaattctt tcctttaggt
     1081 tggagatatg ttcctcaaac ataaagttgc tgaagatctc aagattgccg gattcccttg
     1141 tctggatgag ggattcaata tatttgactt tgttttcttt cttaataatt gaagggatta
     1201 accctgcctc gaactgaagc atattcatca gcaggcgggc catacggccg tttccgtcag
     1261 cccacggatg aatggtgaca agtttataat gagcatcgaa actcatttta tagagagaga
     1321 gcgtgtcggt atatgttttg cgggcatcgt tcagccattg gcaaaaatca tccagggcag
     1381 ccggtatttt tgaataattc aaatacgact ttccttcaaa tcctgccgat acattcaaaa
     1441 gtctgagatc tcctttcgca gaagaaaaag agccgagaga tgtgttatat tcactccctg
     1501 tattacgcat aagaatgccc gccagtttct tcagaatttc taccgagata cccctatgct
     1561 ctttagcata cacgaccgct ttctcataag ctgcctttaa atcaagattc atcaactgtt
     1621 cattcatact ccttccggaa gcactgattc cctggtcaaa gagcaattga ttctcgatct
     1681 ctgttaccgt ggagccttca atggcagtag aatgggtaat aatggagtac aggtagaatt
     1741 tgtcataatc tatctgtttg tcaataccca gattcttgta tttggtaatg agctctataa
     1801 gtaacttttc atccatatca caaatatata aataaaaaat tatgtgcagc acattctctg
     1861 gtgaatgtca gacctttatt agattaactg aaataactgt aatcgttaca gttatataaa
     1921 tttaggctgt cttgccgatg gtcttgtttc cacagaaagc cggatctgtt ttaaatacca
     1981 tatggaatgt caagggtcaa atgcggtcag acgctaacgc gctggttgaa gcctcggctt
     2041 acagaatata ctttcctttc cttgtgcggc tgaaagcact tgtgacaaaa ggttctgcct
     2101 cttttattat ctttgcaact tgtatccaaa aataactcct atgaaacaat ccatcctttt
     2161 attgacagtt cttatcacag ctgcttctgc tacggctcag gagcctgtac aggtacagaa
     2221 aaagccgctg gatcacagtg tttacgacgc ctggaaaagt gttggggcct tttccatgag
     2281 cgaggacggt aaatatgcct cataccttgt ccaggaacag gaaggtgacc gctatgcaga
     2341 agtgctggac ctggtgacac tgaagaagca gcgcattgaa agggccactc agccaaagct
     2401 gactcccgac ggccggttcc tggttgcctc catcaatccg tttttcaagg aaacaaaaga
     2461 agcaaaactc aagaaggtca aaaaagaaga gatgcccaag gatacactcg gcatatataa
     2521 ctgtgagacc ggagagctga aaaagatccc ctttctgaag tcggtgaaaa tcggcaaact
     2581 gggaaaaacc catatcgcct tccagtgcga catgcctgcg gatactaccg gcgggaaaaa
     2641 gcccgaaaag atagaaaagg accagggaca agacctgatg gtgtatcacc tggccacagg
     2701 atccacagac acgctcccgg ctgtttctga ttacaatttc agtacgggcg gggacaccct
     2761 gttttttgtc agaaggcccc actcaaagga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn ctttcctggc ggcagctctc cacagacgag caccacattg tgcaggttcc
     2941 gcaggaatat gatgccgact ggggctattc ggtcagcgat tacccctacc agctagaatc
     3001 ctcctggagc tcaaatcccc gctctgatct ctacctgatc aacataacgg acggctcggc
     3061 ctcggccctg ctcaaagacg cctatatttc cggggtaagc gcctcggaag aaggaaaata
     3121 cctggtctgg ttcaacaacc aggaccaaca ctggtattcc tgtgatgtgc aaagccggca
     3181 gatcgtttgc atgaccaagg gtctggatgt ttcttttgcc aacgagctgc atgatacacc
     3241 ggaaatggcc tcttcctacg ggaacgaagg ctggctggaa ggagaccagg caattttcat
     3301 caacgaccga tatgatatct ggcagatgga tccttcgggt gaaatggccc ccgtgaacct
     3361 tacagacggg ctaggccgca gggagaacct taccttccgt atcgtaaggc tggatgagac
     3421 gttgttgcct gccggtaccc cgggagtgaa aaaagaaccc ttcaagccga aggaaaccat
     3481 ttacttttct gtatttgaca acaagaccaa ggaaagcgga tactattaca aagagatggg
     3541 caaaaggcgc ccggtgatga cacgctgggt ccaggagcct gtgacctttg cctacctgac
     3601 ccgttcaaaa gacggcaagg taatgatcta cagcaaacag aactttgtaa actcacctga
     3661 tgtgtggttt acaaaagaca aattcaaaac ccaggtaagg atcaccgaca tcaacccgca
     3721 acaaaaagaa tacaactggg gcaccgcagg gctggttcac tggaaatctg ccacaggcct
     3781 ggacctggag ggtatcctgt acaaaccgga aaactttgat cccgcaaaga aataccccat
     3841 gatcgtgtat ttttatgaaa ggacatcaca gacactccat acgtacaggg ctccggcacc
     3901 cagccgctca accataaaca tacccttttt tgttagcaat gaatacatcg tatttgttcc
     3961 cgatattgct taccagacgg ggcatccggg caaaagcgca ctggactgta tcgtccccgg
     4021 tgtgaaaaaa ctgattgagg agaattcatg gattgacgca gacaacattg ccatccaggg
     4081 acaaagctgg ggaggctacc aggtggctta catggtcaca cagccccagg ttttcaaatg
     4141 gaaagcagcc ggcgcaggag ctcccgtatc caacatgacc agtgcctacg gcggtatacg
     4201 ctggggttcg ggcatggtcc gccagttcca atatgagcac tcccagagcc gcatcgggca
     4261 aaatctgtgg gacggattcg atctttacat agaaaattcc cctttgtttt ttgcagataa
     4321 ggtggaaacc cccttgctca tcatgcataa cgacaaagac gaggccgttc cgtggtacca
     4381 gggcattgaa ctcttcacgg ccttgcgcag gttgggcaaa cccgt
//
LOCUS       DAOSOF010000146         4346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20245_length_4346_cov_4.476113, whole genome shotgun sequence.
ACCESSION   DAOSOF010000146 DAOSOF010000000
VERSION     DAOSOF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20245_length_4346_cov_4.476113"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLM68_07660"
     CDS             <1..181
                     /locus_tag="PLM68_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain protein"
                     /protein_id="HOT55268.1"
                     /translation="GGSIPVLAELEGILGASPLLMGFGLERDTIHSPNESYLLSQFHK
                     GVESIILFYKYYTGA"
     gene            187..1629
                     /locus_tag="PLM68_07665"
     CDS             187..1629
                     /locus_tag="PLM68_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOT55269.1"
                     /translation="MKLLFILTCLAAGLQEGLPVRFSSNFDSGSIGEVSLIDSVWVRP
                     SRTDSVLHLSYEIISRLDPANPANPELQPSGRWFYFMMEGVKGKHIYLDFKNTDPMRA
                     VYSYDGKHFQRFEHYQASLRKVSSFFSRDTVYVSYFIPYGWDKLQERLRQWTASPFVV
                     MDTLGTSMMGLPVQMLAITDPQVPEERKNRIWIHARVHPSESPANWQLEGLLDRLTDN
                     SPVARQYLRQSVFYVVPFANPDGVYGGYSRSNARGINQEINWDHNDSVTSVEVQALRA
                     RMDSLTRERPFDLVLNMHAQSDDKISYYVPTSASTYPEYHEKAMRLAYLTTDNNPYFF
                     MEDMFYSSPAPRYVEGWIWNRTKGKTLAMTIEMPYSFYNENPDRPWVTVTSLRENGEL
                     MLRAISDFFGWNIPGRYILKGTLRGKKTIFRHENLPAGEYQVFIWKDAWVPCDTVIKK
                     RTGMFRCVIRTDGEPYTGPLRVSEKATLRN"
     gene            complement(1613..3151)
                     /gene="rny"
                     /locus_tag="PLM68_07670"
     CDS             complement(1613..3151)
                     /gene="rny"
                     /locus_tag="PLM68_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT55270.1"
                     /translation="MNYTYISAAIFLVVGFALGYAFFRMVILKKKAKTLLKEAETEGE
                     SIKREKILQAKEKFLQLKTEHEQLIIERNNKIAQAENKLKQREIAMNQQNAEIQRKSK
                     EVSSLKDSLTAQLELAEKKVEEYEKLRLQQIEKIESMASMSADQAKALLMETMKEEAK
                     SQAMSYISEVMDEARLTAGKESKRIIVNTIQRVATETAIENAVTVFNIENDEIKGRII
                     GREGRNIRALEAATGVEIVVDDTPEAILLSAFDPVRREVARLALHQLVTDGRIHPARI
                     EEVVAKIQKQVEEEIVETGKRTCIDLGIHGLHAELVRLVGKMKYRSSYGQNLLQHSRE
                     VANFCSIIAAELGLNAKLAKRAGLLHDIGKVSDDDPELPHAILGMRLAEKYKERPEVC
                     NAIGAHHEETEMASMIAPLVLVGDAISGARPGARREVIESYIKRLKGMEDLALSYPGV
                     TKTYAIQAGRELRVIVGSEKVSDQDAEKLSQDIAHKIQDEMVYPGQVKITVIRETRAV
                     NYAK"
     gene            complement(3402..3692)
                     /locus_tag="PLM68_07675"
     CDS             complement(3402..3692)
                     /locus_tag="PLM68_07675"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT55271.1"
                     /translation="MSEDKLSIKISIADRYYPLRIAREDEEMVRAAAQRINQKIEQFN
                     VKYSGQDTQDALAMAALQFVLSLLKYEKDDETSQIVTEIASLDNFLREYLER"
     gene            complement(3702..3995)
                     /locus_tag="PLM68_07680"
     CDS             complement(3702..3995)
                     /locus_tag="PLM68_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55272.1"
                     /translation="MKPQHAAIINPLKQKIETLISLHEKALTENRELNSKLQTLSEEQ
                     NIYKKEKEELEQKVQKLQMAGAFLKTSEDTREAKQRIGKLIREIDKCLTMLNR"
     gene            complement(4050..>4346)
                     /locus_tag="PLM68_07685"
     CDS             complement(4050..>4346)
                     /locus_tag="PLM68_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT55273.1"
                     /translation="MVKKGGLLLYDPNGISRHPVRKDIKVCSIAAADLSNRMEMSKAF
                     NMVVLGAYLKMKPILQKDNVIKGLKKSLPERYHDLIPMNEKAIELGMKEVKEVI"
BASE COUNT          939 a         1075 c         1013 g         1319 t
ORIGIN      
        1 gggaggaagc attccggtac ttgccgagct ggaagggatc ctgggagctt caccgctgct
       61 tatgggcttc ggcctggaac gcgataccat ccattctccc aacgagagtt acctgttaag
      121 ccaattccat aagggagttg agtccatcat cctcttttac aagtattaca cgggggcata
      181 aaagttatga agttgctttt tattctgact tgtcttgcag ccgggctgca ggaaggtctt
      241 cctgtcaggt tttcgtcgaa ttttgattcc ggaagcatag gggaggtgtc ccttatagat
      301 tctgtctggg tcaggccttc aaggaccgat tctgttctcc atctgtccta tgagattatc
      361 tcacgtttgg atccggccaa tccggccaac ccggaactgc agcccagcgg tcgctggttc
      421 tacttcatga tggagggcgt gaagggaaaa cacatctacc tggatttcaa gaacacagat
      481 cccatgcggg ccgtttattc ctatgacggg aaacatttcc agcgctttga acattaccag
      541 gcttcccttc gcaaagtatc ctctttcttt tcccgtgata cagtatatgt gtcctatttc
      601 atcccctatg gatgggacaa attgcaggaa cgactccgac aatggactgc ttctccgttt
      661 gttgtgatgg ataccctggg aaccagcatg atgggattgc ctgtccaaat gcttgccata
      721 accgatcccc aggttcctga agaaaggaaa aacaggatat ggatccatgc tcgtgtacat
      781 cccagtgaat ctcccgcaaa ctggcagctg gaagggcttc tggaccgttt gacggataat
      841 tctcctgtgg caaggcaata cctccgccag tcggttttct atgtggttcc ctttgccaat
      901 cccgacgggg tatacggggg atattcccgt tccaatgccc gcggcatcaa ccaggagatc
      961 aactgggacc acaacgactc cgttacttct gtagaagttc aggccttacg tgcacggatg
     1021 gattccctga cccgtgagcg tccgtttgac ctggtcttga acatgcacgc ccagtcagat
     1081 gacaagatca gttattacgt gcccacttca gcatccacct acccggaata ccatgaaaaa
     1141 gccatgcgcc tggcatacct gacaacagac aacaaccctt attttttcat ggaagacatg
     1201 ttttattcat ctcctgcacc ccgctatgta gaggggtgga tatggaacag gacaaagggg
     1261 aaaacgctgg ccatgaccat tgaaatgcct tattcgtttt acaatgaaaa ccccgacagg
     1321 ccatgggtaa ccgtaacctc actcagggag aacggggaat taatgcttcg ggccatttcc
     1381 gattttttcg gctggaatat tccgggacgc tatatactca aaggtacctt gaggggtaaa
     1441 aagaccattt tccgtcatga gaacctgccc gcaggagaat accaggtctt tatctggaag
     1501 gatgcatggg ttccctgtga tactgtcata aaaaaacgca ccggaatgtt ccggtgcgtt
     1561 atccgtaccg atggagaacc ctacacggga ccccttcggg taagcgaaaa ggctactttg
     1621 cgtaattgac cgccctggtt tcccggatga ccgtgatttt cacctgtccc ggatagacca
     1681 tctcgtcctg gatcttgtgt gcgatatcct gtgacagctt ttcagcatcc tggtcgctta
     1741 ctttttcaga tcccactata acccgaagct cacgccctgc ctgtatggca taggtcttgg
     1801 tcacacccgg atatgacaag gcgaggtctt ccattccttt aagccttttt atgtaggatt
     1861 caatcacttc cctccgtgct cccggacgtg cgcccgatat ggcatccccg accaggacca
     1921 gcggtgcgat catggaggcc atttctgtct cttcgtgatg ggcgcctatg gcattgcaca
     1981 cctcgggtct ttctttgtat ttttcggcca gacgcatgcc caggatggca tggggcagct
     2041 caggatcgtc atcggatact ttgcctatgt cgtggagcaa tccggccctt ttggccagtt
     2101 ttgcgttcaa gccgagttca gctgcgatga tggagcagaa attggccact tcacgcgagt
     2161 gttgcaacag gttctgcccg taagaggaac ggtatttcat ctttcccacc agccggacaa
     2221 gctcggcgtg gagtccgtgg atacccaggt ctatgcaggt gcgtttaccg gtttccacaa
     2281 tttcttcttc cacctgtttc tggattttag cgaccacttc ttctatgcgt gcagggtgga
     2341 tgcgtccgtc ggtcacaagc tggtgaagtg ccagacgggc cacttcgcgg cggaccgggt
     2401 cgaatgcaga aaggagtatg gcctcggggg tgtcgtccac gacgatttcc acaccggtgg
     2461 ctgcttccag ggccctgatg ttccttcctt cacggcctat gatgcgtcct tttatctcgt
     2521 cgttttcaat attgaaaacg gtgacggcgt tttctatggc tgtttccgtt gccacccgct
     2581 ggatggtgtt cacaataatg cgtttggatt ccttgcctgc tgtgagccgg gcttcatcca
     2641 tgacttcgct gatgtatgac atggcctgac ttttggcctc ttctttcatg gtttccataa
     2701 gaagggcctt tgcctggtca gcggacatgc tggccatgct ttcgattttt tcaatctgtt
     2761 gcaggcgcag tttttcatat tcttccacct tcttctcggc cagttccagc tgggcggtca
     2821 ggctatcttt cagggaggag acttctttgc ttttacgttg aatttcggca ttttgctgat
     2881 tcattgcgat ttcccgctgt ttcagcttgt tttctgcctg ggctatcttg ttgtttcttt
     2941 caattattaa ttgttcatgt tctgttttta gctgaaggaa tttttctttt gcctggagta
     3001 tcttttctct ttttatgctt tctccttcag tttctgcttc ctttagaagt gttttggcct
     3061 ttttcttcag gatcaccatc ctgaaaaaag catagcccag tgcaaatccc actaccagaa
     3121 agattgcggc acttatatat gtgtagttca tggctgttgt tgatatatat tagtttgtgt
     3181 gttgtcataa tagcatataa aaaacccgtt aacataccac tgtttagcaa aaaccctgat
     3241 tagcaaggtt aggaggatct gattttgttg cgaacttccc acgtcccgaa accggaattc
     3301 cccgaagggt aacagagggg cctgttttta acagtcaaga cactccatta agtttaaaat
     3361 gttgagtttc gcaaagagct ggtatgtaac gggtcaacta attatctttc caggtattct
     3421 cttaaaaaat tatccagtga ggcaatctct gtgactatct ggctagtctc gtcatccttt
     3481 tcatatttga gcaggctcag cacaaactgg agggctgcca ttgccagggc atcctgggta
     3541 tcctggccgg aatactttac gttgaactgc tcgatcttct ggtttattcg ctgcgctgca
     3601 gcccgtacca tctcttcgtc ttctctggct atgcgtaagg gataataacg atctgcaata
     3661 cttattttta tggataattt atcttcactc attgtgccgg gttatcggtt aagcatggtt
     3721 aaacatttat cgatttccct gatcaatttc ccgatcctct gctttgcttc tctcgtatct
     3781 tcacttgttt tcagaaaggc tccagccatc tggagttttt gcactttctg ttccagttct
     3841 tctttttctt ttttatatat attctgttct tcgcttaagg tttgtaattt actgttcaat
     3901 tcacggtttt ccgtcaacgc cttttcatgc agtgaaataa gtgtttctat cttctgcttt
     3961 agcgggttga taatggcagc gtgttgaggt ttcatatgca ttgtccgtta agcaaagata
     4021 atgcattttt agcgaataaa aaaagaggtc taaatgacct cttttacttc tttcattccc
     4081 agctctatgg ccttctcgtt cataggtatc agatcatgat acctttccgg tagggacttc
     4141 ttcagaccct tgatcacgtt gtctttttgc aggatgggtt tcattttcag ataggccccc
     4201 aggacaacca tgttgaaagc cttggacatc tccatacggt ttgacagatc ggctgccgct
     4261 atggagcaga ccttaatgtc tttgcgaacc ggatggcgtg atatgccgtt ggggtcataa
     4321 agtaacaggc cccctttctt caccat
//
LOCUS       DAOSOF010000147         4301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20522_length_4301_cov_5.363401, whole genome shotgun sequence.
ACCESSION   DAOSOF010000147 DAOSOF010000000
VERSION     DAOSOF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20522_length_4301_cov_5.363401"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLM68_07690"
     CDS             complement(<1..425)
                     /locus_tag="PLM68_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55274.1"
                     /translation="MKTKQFFALLALIVLTNACGNVKEAHIPPALTSAVTAVSASLDS
                     LSNSLNAAATGLAQVGADTAMVRSELQRLYNECSFGTEFVFTTPEGILQLIEPAAYYP
                     SQGSDISRQAHIIKSFETKLPALSDVFSVVEGFQAVVQPS"
     gene            complement(541..957)
                     /locus_tag="PLM68_07695"
     CDS             complement(541..957)
                     /locus_tag="PLM68_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOT55275.1"
                     /translation="MNGCATSSSKNEDIKATIIALEEQALALWNNGNPDGFLELSTED
                     VVYIDPAFESKLEGKKALEDYYNTIRGKIKIDLYKMIDPVVQVSPGVAVLTYNYEAHR
                     DGMVFTMNCTEVYRLDLNNKWKIIHTHWSFVQPGKM"
     gene            1292..1939
                     /locus_tag="PLM68_07700"
     CDS             1292..1939
                     /locus_tag="PLM68_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT55276.1"
                     /translation="MEKKHEVHNLIILDESGSMNSIKNHVISGLNEIVQTIKGIEKKF
                     PEQEHYITFLTFNGFGIKQLHFADRAREVKKINSQTYNPEADTPLYDAMGYAITKLKL
                     HLMNKTDYHVLVTVFTDGEENASEEYQAQAIKKLVEELKQQHWTFTYIGTDHDVLHAG
                     TSISITNTITFNKNEDGVKDLFLKEKTARYNYSQRIRDKVETGEDYYKELEEEPD"
     gene            2156..4171
                     /locus_tag="PLM68_07705"
     CDS             2156..4171
                     /locus_tag="PLM68_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOT55277.1"
                     /translation="MKETKAAGPLSKLSMAGLLITLGIVYGDLGTSPLYTLRAMLNGA
                     EVIDRDFILGALSCIFWTLTLQTTVKYILITLKADNKGEGGIMALFALLKKRKNWFYI
                     VAIVGGCALLADGIITPAITVTSAIEGLRILKPGIPVVAVVFVILSVLFFIQQFGTAF
                     LGKSFGPLMLFWFTMIGVLGLVQLVRVPEVLLAVNPYYAIKLLSAYPNGFLLLGAVFL
                     CTTGAEALYSDLGHCGVRNIRISWIFVKTALLLNYFGQGAWIITHARDITPELNPFYA
                     IMPEWFLIPGILVATAAAIIASQALISGSFTIISEAISLNFFPKVRISYPSSLKEQMF
                     IPLVNAILYLGCCFVVFYFRESSGMEAAYGLAINLAMLATTVLLMFYLKDKFPLYLRI
                     LCGVVFFAVELSFLSANLFKFSDGGWITIMFTGFFALVMYVWNRGRRIKNSFITYVKI
                     KDYLPVIKSLSGDETVPMYASNLVYITHANRVTDIENKILYSILRKQPKRADTYWLLH
                     VDIVDEPHVREYEVTELIPGVLIRIDLRMGYKVPVKASLYFHNIVFDLMAEHKIDLIS
                     SYPSLRRHNVLSDFRYVVIDRVPNKDYEFSHNQKLIMNLYFRISKIGMSDVRYLGLDV
                     TNVNVEMVPLLARSEIIYNEKQPPMRTKESKRIHTADTSFTRVRHDN"
BASE COUNT         1151 a         1011 c         1001 g         1138 t
ORIGIN      
        1 gatggctgga ccactgcctg aaaaccttca actaccgaaa acacatcgct cagggcgggc
       61 agtttggttt caaaagactt aataatatgg gcctgccggc tgatgtcgga accctgggag
      121 ggataatagg cagccggctc tatcagctgc aggatgcctt ccggagttgt gaaaacgaat
      181 tcagtgccaa aggaacattc attgtagaga cgctgcaact cggaacggac catcgcggta
      241 tcggcaccca cctgggcaag gccggtggct gcggcattta gtgagttgct aagactatcc
      301 aacgaagctg aaacagcagt tacagcagat gtcagggcag gggggatatg ggcttcttta
      361 acgttaccac aggcattagt tagtacaatt aatgccagaa gagcaaaaaa ttgtttggtt
      421 ttcatgatgt tatccgatgt gagacatcat attttgacgt agcagtattg tattttcgca
      481 ggctcaaaaa tgattcaggc tatgatactg taaaagaggt gtgagagtcc ctctcatacg
      541 ttacattttt ccgggctgta caaaagacca gtgtgtatgt atgattttcc acttattgtt
      601 tagatctagc ctgtatacct ctgtgcagtt cattgtgaat accatgccat ccctatgagc
      661 ctcataattg taagtaagga cagcaacccc aggcgagact tgtactacag gatcgatcat
      721 tttataaaga tcgattttaa tctttcctct tatggtgtta taataatctt ccagcgcttt
      781 tttaccctct agtttgctct caaaagcagg gtcgatataa acaacatctt cggtagacaa
      841 ttcaagaaat ccgtcgggat ttccattatt ccaaagtgct aaagcctgtt cttcaagggc
      901 aatgattgtg gctttaatgt cctcattttt tgaggatgac gtggcacatc cgttcattat
      961 tagtaaaatt agaatgaata aaatccgatg tttcatcttt atattttcag gcatgtttaa
     1021 tggggcaaaa ttaatctttt ttggataagc cggggcttcc ccttgatttt gctgccttct
     1081 ccggttgctg acaccgcaag gtaagggaag cctcggcata aggaaaaaag cttcccgtcc
     1141 ttatggtggg tagcgaccat ttgtcgaatt aaggaaaagg ttgaagcccc ggcttgtaaa
     1201 aagcaggttc ttcttcgtac gtcacatttt ggcgtagtgg acctgtattt ttgcgggaaa
     1261 ccatacagca acaacagtta acatatacat tatggaaaag aaacacgagg tacacaacct
     1321 gatcattctg gatgaaagcg gctccatgaa cagtattaaa aaccatgtta tcagtggttt
     1381 gaatgaaata gtgcaaacca tcaaaggaat agagaaaaaa ttcccggagc aggagcacta
     1441 cataacattt ctgaccttca acggatttgg cataaaacaa ctacattttg cagacagggc
     1501 aagagaggtg aaaaaaatca acagtcagac gtacaatccc gaagcggata ccccgttata
     1561 cgatgccatg ggatacgcca tcaccaaact gaaactgcac ctgatgaaca aaacagatta
     1621 tcatgtactg gtaaccgttt tcactgatgg ggaagaaaat gcatcggaag aataccaggc
     1681 acaggccatc aaaaagctgg tcgaggaatt gaagcagcaa cactggacct tcacctacat
     1741 cgggactgat catgatgtac tccatgcggg cacgtctatc tccataacca acacgatcac
     1801 tttcaataaa aatgaagatg gcgtcaaaga ccttttccta aaagagaaaa cagcccggta
     1861 caactacagt caaaggatcc gtgacaaagt ggaaaccggg gaggactact acaaggagct
     1921 tgaagaggag cccgattaac aggataagta ctatgtcacg tgcctgaagc tgtaatcgca
     1981 acctacgggt cgctgtcaca acaaagaacg gaaagccttg gcatacaata aaaagctcca
     2041 tgttccttat ggcgttgcag ccaccgtttt ttaggacacc gaggtcccac ctctctgctt
     2101 tttatataca tttgtgcggt ttattaaagc gcgatcaacc gccgcataaa aacatatgaa
     2161 agaaacaaag gcagccggtc ccctgtcgaa gctatcgatg gccggtctgc ttatcacact
     2221 ggggattgta tacggagact tgggaacctc gccgctctac acgctcaggg ccatgctcaa
     2281 tggggccgaa gtcatagaca gggattttat tctgggagcc ctgtcctgca tattctggac
     2341 actcacgctg caaaccactg ttaagtatat tttaataacc ttaaaggccg ataacaaagg
     2401 ggaggggggc ataatggcct tgttcgccct gctcaagaag cgaaaaaact ggttttatat
     2461 tgtggccata gtgggaggct gtgccctgct ggcagacggg atcatcactc cggccattac
     2521 cgtcacctcg gccattgaag gcctcaggat cctgaagcca gggattcccg tggtggcggt
     2581 ggtctttgtg atcctgagcg ttctgttttt cattcagcaa tttggcacgg cgttcctcgg
     2641 caaatccttt ggacccctca tgctcttctg gttcacgatg attggtgtcc tgggcctggt
     2701 ccagctggtc cgggtccctg aagtccttct ggccgtcaat ccctactacg ccataaaact
     2761 gttgtctgcg taccccaacg gattcctgct ccttggggcc gtttttcttt gtactaccgg
     2821 ggccgaggct ttgtattcgg accttggtca ttgcggggtg cgtaatatca ggatttcctg
     2881 gatctttgta aagacagccc tgctgctgaa ttattttgga cagggagcct ggatcattac
     2941 gcatgcccgg gatattactc cggaattaaa cccattttac gccataatgc ccgaatggtt
     3001 tctgatcccg ggaattcttg tggcaacggc agcggccatc atagccagcc aggcgctaat
     3061 aagcggatcg ttcaccatca taagcgaggc catatccctc aatttcttcc ccaaggtacg
     3121 cataagctat ccatcgtcgc taaaagaaca gatgttcatt ccgcttgtca atgcgatcct
     3181 ttacctgggc tgttgttttg tggttttcta tttcagggaa tcttccggaa tggaagcggc
     3241 ttacggactg gccattaacc tggctatgct ggcaaccaca gtgctcctga tgttctacct
     3301 taaagacaaa tttcccttat atctaaggat tctgtgcggt gtggtgtttt ttgcggttga
     3361 actctcgttc ctgtctgcca atctcttcaa gttcagcgat gggggctgga ttaccatcat
     3421 gtttaccggg ttttttgccc tggtaatgta tgtttggaac cgcgggcgcc ggataaagaa
     3481 tagctttata acctatgtga agataaagga ttacctgcct gtgatcaaat ccttgtccgg
     3541 tgacgagacc gtacccatgt acgccagcaa cctggtttat atcacgcatg ccaaccgggt
     3601 aacagatatt gaaaacaaga tcctatattc cattctccga aagcagccta agcgggcaga
     3661 cacctactgg ctccttcatg tggacattgt ggacgaacca cacgtcaggg aatacgaggt
     3721 tactgaactg attcccggag tattgatacg tattgacctt cgcatgggat acaaggtgcc
     3781 ggtcaaagcc agcctgtatt ttcacaacat tgtctttgat cttatggctg agcacaagat
     3841 tgacctgata agctcctatc cttccctcag gcggcataat gtactaagtg atttccgcta
     3901 cgtggtcata gaccgtgtgc ctaacaagga ctatgaattt tcccacaatc agaaactgat
     3961 catgaatctg tacttccgca taagcaagat tggcatgtca gatgtgcgtt acctgggcct
     4021 ggatgtcact aacgtgaatg tcgagatggt tcccctgctg gcccgctcag aaatcatcta
     4081 caatgaaaaa cagccaccta tgcgcaccaa ggagtccaaa agaatccaca ccgccgacac
     4141 ctcgttcacc cgcgtccggc acgataatta gttttcttac aatcccttct tgcgacagga
     4201 tggataaaag ccaaggcttt gttcattgat attccagagg caacctcctt gcccgggtcg
     4261 cctgctcata agcgtatccg attttcagga gcttgtgttc t
//
LOCUS       DAOSOF010000148         4295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20556_length_4295_cov_5.925472, whole genome shotgun sequence.
ACCESSION   DAOSOF010000148 DAOSOF010000000
VERSION     DAOSOF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20556_length_4295_cov_5.925472"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="ispF"
                     /locus_tag="PLM68_07710"
     CDS             <1..409
                     /gene="ispF"
                     /locus_tag="PLM68_07710"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT55278.1"
                     /translation="QIDHTKGCIAHSDGDCLLHALCDALLGALAMGDIGSHFPDTSEE
                     YKGIDSRILLERTYAMVRKARYYLGNADCTVCLQAPRIAKYIPRMKEVLAGILDVAPE
                     CISIKATTTEHLGFVGREEGIAVYATVLLWPEV"
     gene            466..539
                     /locus_tag="PLM68_07715"
     tRNA            466..539
                     /locus_tag="PLM68_07715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Met,seq:cat)
     gene            complement(1150..2610)
                     /locus_tag="PLM68_07720"
     CDS             complement(1150..2610)
                     /locus_tag="PLM68_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55279.1"
                     /translation="MVEIQYKIHDEFAVEFKQRFLVRRKVQKNVFVLNTWFFIPNSLD
                     INPQTYGKDQFYRDVKSNVRMITPVYILRDLAEKDAVPFRHLEKAFRDVASAPLRRNA
                     SEYIYQIKMVSAIIKSALRDHAKMILRGQSPDNTAWLCSQYADSAKAILACYRNLKSI
                     ITVPTVPDELQDHFRFGDEALSQVVVLYALRILKFLKGKSKYSEEEQRIHDLVVGEYA
                     YKREAGYNVLDARDPENNRDLVFRYGLLKKYIESDLFVTLNKKRDGVAIEQIYYSIAA
                     GVAMIFATVVAFIFQRRFGSVSIPLFVALVVSYMLKDRIKELMRYYFAYKLKFKYFDH
                     KAVVRIKDEEIGWIKEGMDFISPGKIPQEVMNLRNKNNLMGSEFAILDEKIILYRKLV
                     NIDSHKLAENNIYHISGINDIVRFHVNRYTQKMDNPEIPLLKIDEETGDLVTLNCAKT
                     YFLHIVMQVQSEGKSSFHAYKVIVSRNGIQGITFLE"
     gene            complement(2621..4204)
                     /locus_tag="PLM68_07725"
     CDS             complement(2621..4204)
                     /locus_tag="PLM68_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HOT55280.1"
                     /translation="MTFDPLDMNNYRIEKLPKMQKSGFERWMSILGGPLSVVMFVLIY
                     WFSDISFLNEISTEGLSDTALQRLQALGTDGFSRINYAMLAIFVAAIILWITEAIPNY
                     LTSLIVILSIVLTGVTTEKTAFAQLGHPVMWLNILSFILASMLVKTQVAKRFALWFVL
                     RFGRNSFGVMISFIVINLVLSAFISATTAKAAILLPIFMVVAAIYGATGGENRNNFGR
                     NLVLQNLFQINLGANGFMTGSGATLLAASLIAGAMGETSFGYTKWLVPALPMSILLIL
                     IAWFVGTRVVFRLKPEEKKPRIEGGMERLRQELHSLGPMKTEEYKAVAIFVCVLGLWA
                     TETLHGISPTAVAFIGAVVALIPKFGVVKWNDVDIPWHLLLFSAGAYTLGAGLDASGL
                     PGILVSKAFAAIGISQQTPFWVLYLLLTGTMLFSALLFQSKTMQTLIFVPIAISVAQQ
                     FGYPVMSLAFPVAMLVGHVYVLPFNSKPAALLYTTNHYSWSDTFKFGIIMMVISWFMI
                     ILWGETVLRWLGYTNGVFF"
BASE COUNT         1082 a         1075 c         1083 g         1055 t
ORIGIN      
        1 acagattgac cacacaaaag gatgcatagc ccattcggac ggtgactgtc tcctgcatgc
       61 cctgtgtgat gccctgctgg gggctttggc catgggggat attggctccc acttccccga
      121 tacatctgaa gaatacaagg gaattgacag caggatactg ttggagcgga cttatgcgat
      181 ggtcaggaaa gcgcgttact accttggaaa tgcagactgc accgtttgcc tgcaagcccc
      241 ccggatcgca aaatacattc cccggatgaa agaagtcctg gccgggattc tggatgtagc
      301 tccggaatgc atttccatca aagctaccac cacagaacac cttggttttg tggggcgtga
      361 agaagggatc gctgtatatg cgacagtcct gttgtggccg gaagtctgaa atcaaattcc
      421 cgtttgtgtg tttaaaaata atgattactt ttgcagctcc gtaacggcga ggtagctcag
      481 tcggttagag cgcatgattc ataatcatga ggtcacgagt tcaattctcg tcctcgctac
      541 acaaaaaagc acttacagtc ttgtgagtgc tttttttttg ccccgaaacg aggtggaacc
      601 tcctgttcca gtcaggtcgc tgccatcaca aagaaccagg tactcttttt ggcaatgccg
      661 agactctcca tgcttttgct gccagttgcc gtcactgcca ctacaaggaa cagagagttt
      721 ttttggggta cgccgaggct ctccgttcca ggtgagtgcg tcgcacacat tgttcttttg
      781 cctgtcatac aatgtgttat acatttcgta cccccatggg tatgaattgt gtaagtagtt
      841 gataatgagt caaacgaatc aagtgtgcga ggcacccatt agcttgacag ggaggtggta
      901 agtagccgaa acctcggcta taccattaac tggcacaata acagcgcctt aattagcgcc
      961 aaaagccttt ggcgcaccgc cttgaatgat ccgcattgaa agaccaccgg acaactgtta
     1021 gggcagcaat gcgcataatt gcaaggtcgt ttgagtgcta agaacggagt gcctcggctt
     1081 aaaagaaaaa gcactccgtt cttagcactc aagcgtccgc ctgcagtaaa gggttgcagc
     1141 tctggcaagt tactccagga aggtaatccc ctggatccca ttacgtgata cgatcacttt
     1201 gtaggcatgg aaggaagatt tcccttccga ttgcacctgc atgactatgt gcagaaaata
     1261 ggttttggcg cagtttaagg ttaccaggtc ccccgtttct tcatcgattt taagcagggg
     1321 aatttccggg ttatccattt tctgggtata gcggttcacg tgaaagcgga caatgtcgtt
     1381 aatgccgctg atgtgataga tattgttttc tgccagtttg tggctgtcaa tattgaccag
     1441 cttcctgtac aggatgatct tttcgtccag gatggcaaat tcagagccca tgaggttgtt
     1501 tttgttgcgc aggttcatta cttcctgtgg gatcttgccc ggggaaatga agtccatccc
     1561 ttctttaatc cagccgatct cctcgtcttt tatccgcaca acggccttgt gatcaaaata
     1621 tttgaattta agtttgtagg caaagtaata ccgcatcagt tccttgatcc tgtctttcag
     1681 catgtaactg accaccaggg caacgaacaa gggaatggat acgctcccga aacggcgctg
     1741 aaaaataaaa gccaccaccg tggcaaagat catggccacc ccggcggcaa tgctgtagta
     1801 gatctgttca atggccaccc cgtcacgctt tttattcagg gtcacaaaaa ggtcgctttc
     1861 tatgtacttc ttgagcaggc cgtaccggaa caccaggtcc cggttgtttt ccgggtccct
     1921 ggcatccagc acgttgtaac ccgcctcccg tttataggca tattccccca ccacaagatc
     1981 gtgaatgcgt tgctcttcct ccgaatactt gctttttccc ttaaggaatt tcaatatccg
     2041 aagagcgtaa agcaccacca cctggctgag cgcctcgtct ccaaaacgaa aatggtcctg
     2101 caattcatcg ggtacggtgg ggacggttat gatgctctta agattgcggt agcatgccag
     2161 gatggccttt gccgaatctg cgtactggga gcagagccac gcggtattgt ccggcgactg
     2221 acccctgagg atcatcttgg catggtctct cagggcactt ttaatgatgg ccgagaccat
     2281 tttaatctgg taaatgtact cagaggcgtt tcttcgcagg ggtgcggagg caacatcacg
     2341 gaatgccttt tccaggtgac ggaaaggaac ggcatctttt tcggccaggt cccgcaatat
     2401 gtatacaggg gtgatcattc tgacgtttga tttcacgtcg cggtagaact gatctttccc
     2461 atatgtctgc ggattgatat ccaggctgtt gggtatgaaa aaccatgtat ttaacacaaa
     2521 aacgttcttt tgcaccttac ggcgcacaag aaaccgttgt ttaaactcaa cggcgaattc
     2581 atcgtggatt ttatactgga tttcaaccat aagctaactc ttaaaaaaac actccgttcg
     2641 tatatcccag ccagcgcaac actgtttctc cccacaagat aatcataaac cagctgatga
     2701 ccatcatgat gatcccgaac ttgaatgtgt cgctccagct gtaatggttc gttgtataca
     2761 aaagggctgc aggtttggaa ttgaacggca ggacataaac gtgtccaacc agcatggcca
     2821 cgggaaacgc cagcgacatg actggatacc cgaactgctg agcgacactg atggcaatgg
     2881 gtacaaaaat gagcgtctgc atggtttttg actggaacag cagtgcgctg aagagcatgg
     2941 tgccggtaag cagcaggtaa agcacccaga atggggtctg ctggctgatc ccaatcgccg
     3001 caaaagcttt tgaaaccaga attccgggaa ggcccgaggc gtccaggccg gcaccaaggg
     3061 tgtaagctcc ggcggagaaa agcaacaggt gccatggaat atccacatcg ttccatttga
     3121 ccacgccgaa tttcgggatg agtgccacga ccgccccgat aaaggcgacg gcagtgggac
     3181 tgatgccgtg cagcgtctcg gtggcccaca atcccagcac acagacgaaa atggcaacgg
     3241 ctttgtattc ctcggtcttc atggggccca ggctgtgcag ttcctgcctg aggcgctcca
     3301 tgcccccttc tatgcgtggc ttcttttcct cgggtttcaa tcgaaaaacc acccgggttc
     3361 ccacaaacca ggcgataagg ataagcaata tgctcatggg caaagccggt acaagccact
     3421 tggtatatcc gaaggaagtt tcacccatgg caccggctat gagtgaagcg gccagtaagg
     3481 tggccccgga tccggtcatg aatccgttag ctcccaggtt tatctggaac agattctgca
     3541 ataccaggtt acgtccgaaa ttattcctgt tctcacctcc ggtagctccg tatatggctg
     3601 cgaccaccat gaatatggga agcaggatgg cggctttggc cgtagtggcc gagataaacg
     3661 cggataacac caggttgatg actatgaaac tgatcatcac cccaaaggag tttcttccga
     3721 aacgcagcac gaaccaaagg gcaaaacgct tggctacctg tgtcttgacc agcatgctgg
     3781 ccaggatgaa ggacaggatg ttcagccaca tgacagggtg ccccagctgg gcaaaggcgg
     3841 tcttttccgt ggtgaccccc gtaagcacaa tggagaggat cactatcagg gaggtcaggt
     3901 agtttggaat ggcctcggtg atccataaaa taatggcggc cacaaaaatg gccagcatcg
     3961 cataattgat acgggagaac ccgtccgtcc ccagcgcctg caaacgctgc agggccgtgt
     4021 cggacaggcc ttccgtagaa atttcgttca ggaaagagat gtcggagaac cagtagataa
     4081 gtacaaacat cactacggaa agaggacccc ctaaaatgga catccatcgt tcaaagcccg
     4141 acttctgcat tttagggagc ttctcgatcc tgtagttatt catgtccagc ggatcgaatg
     4201 tcatgtcatt tccagttttt aaatggattc ttcatcaaca tggaattgta ataccgggta
     4261 tcgcccgtga cttccgcacc cagccaggaa ggttt
//
LOCUS       DAOSOF010000149         4283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20629_length_4283_cov_3.698912, whole genome shotgun sequence.
ACCESSION   DAOSOF010000149 DAOSOF010000000
VERSION     DAOSOF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20629_length_4283_cov_3.698912"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1238
                     /locus_tag="PLM68_07730"
     CDS             306..1238
                     /locus_tag="PLM68_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOT55281.1"
                     /translation="MELWKPTDDENRKDTSEHTVRTKVERHRLVADKAVVVRKFHPGS
                     FGYRPGRSAHDAVDQCAKSCRERWLKAPVCNQAGELVTRSKGTPQGGVISPLLANLYL
                     HEAFDWWMAETQPRIAFERYADDIVIHTRSREQSDFILDKIKVRLAEYSLEVHPEKTK
                     TVYCYRTARFYKGGKDMPVSFDFLGFTFKPRLCAKPNGEKFWGFRPAISSKSRKKIIG
                     KLRELAIQNWVFWDIQKIAAALAPMIRGWIHYYGRYRISELQWVFRSLNSRLAKWARK
                     LYKLRTYAKSYGWLKRMIKWYPNTFVHWTYGFTG"
     gene            1353..2228
                     /locus_tag="PLM68_07735"
     CDS             1353..2228
                     /locus_tag="PLM68_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55282.1"
                     /translation="MGGLLTYCVAGHVFGIQTPDKTVTRGILSSYASFTVENSTDSGH
                     LFYFQGNRQVDIPDVPCDDVVQRDTVTYSLYHTVQGITASMQQNGREHRLFASPGYQE
                     IISDVTLLSGNEAPFINSFLRLAYGVRAVHRQTIKVHASVIELNGKALVFLGKSGAGK
                     STHSRLWLQHIPGCTLLNDDEPILRIFEDGAVRVYGAPWSGSTPCYRNDWAEVAAIVH
                     LHQSPDNKLSRMTPLEGLSSLFKSTALLRSDRENKDKVLDVVTGVVERIPVYRLACRP
                     DHEAVLLSRSLLPKE"
     gene            2234..2698
                     /locus_tag="PLM68_07740"
     CDS             2234..2698
                     /locus_tag="PLM68_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HOT55283.1"
                     /translation="MIIPNDILFPHIREEIRAGHSVRIQTRGNSMLPLIRPGTDEIEL
                     SPLDKRSLQKGNIVLARSEDGRYLVHRIEKVKGEMVFIRGDGNASARETCTVNNFFAQ
                     LTGIFRKGKKIEKNSFQWKFAQYGWPSSPLLRRICLGVFRRLHCLHQRIKSN"
     gene            2700..2969
                     /locus_tag="PLM68_07745"
     CDS             2700..2969
                     /locus_tag="PLM68_07745"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOT55284.1"
                     /translation="MQIKKNIRIKQIGDEKILVSNDGKNLDYTRIISLNQSAAFLIEE
                     SMEKEFTVQEWAHRLTDRYDIDYALALSDAQKVVDKLKQAGVLCE"
     gene            complement(3122..3355)
                     /locus_tag="PLM68_07750"
     CDS             complement(3122..3355)
                     /locus_tag="PLM68_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55285.1"
                     /translation="MKKLKQLSTAFLSWLVISLLFVTGLNSCLKPEDPADGKEDPSKP
                     GEFDPTLRATDFPEEMAFPADSVSLGETSLPAE"
     gene            3375..>4283
                     /locus_tag="PLM68_07755"
     CDS             3375..>4283
                     /locus_tag="PLM68_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOT55286.1"
                     /translation="MEENFTRLGQRFTRLGQRLARYGLDGFVLALITAILVAYLWPGP
                     GVYRGMVDLQDINRVGIAMIFFFYGLRLNPAQLRTGLANWRLHVVTQAVTFILFPALV
                     LLVKTLAGGTWDAPLWSGTFFLAALPTTISSSVVLVSMGRGNVPAAIFNASVSSLLGI
                     FLTPLWMGILLVGYKGGFSPTQAILQLSLQVLLPVVAGMMLHKRYGILTVRHPRTFRL
                     FDQGVILLIVYVSFAESFFNKMFGGYSPWHILLLCAAMLAPFMPISGNGPIPNISSGS
                     RTALMTAEVSMIMPGVKVSPVARTMLF"
BASE COUNT         1099 a          981 c         1175 g         1028 t
ORIGIN      
        1 ttgaaaacga ctaagaaccg tattgaaaga ggtatgagtg ctgaggtcga gcgaacagac
       61 tggccgggct acccgacaga gaacgacaag gaaccggaat aaagacgatc caccggcagg
      121 gaccaacagg ggaatctgct aaaccacccg acggtgcttt tatccaacga taaaggtatt
      181 gagaggatcg ggaaaatcgg agtaacctag tctattccgg gaagagtgca ggaataacct
      241 ggaaaccaag ctgcctgcac agacattcgg cataaaggct gcaggacttg tgtataggct
      301 tgattatgga actgtggaag ccaaccgatg atgaaaatcg aaaagacaca agcgaacaca
      361 ccgttaggac aaaagtagag aggcatcggt tggtggcgga taaagccgta gtagtgagga
      421 agttccatcc cggttcattt ggttatcgac caggcagaag tgcccacgat gcggttgacc
      481 aatgcgcaaa gagttgtcgg gagagatggt taaaagctcc tgtctgcaat caggcgggag
      541 aactcgtgac aagaagcaag ggaacgccac agggaggggt tatcagcccg ctgctggcga
      601 acttgtatct gcacgaagca tttgattggt ggatggcgga aacgcagcca cgcattgctt
      661 ttgagcgata tgcagatgat attgtgattc acacgcgaag cagagaacag tcggacttca
      721 tactggataa gataaaagtc agactagcgg agtactcatt ggaagtccac ccggaaaaga
      781 cgaagacggt ttactgttat cgtactgcca ggttttacaa aggaggaaaa gatatgccgg
      841 tatcatttga tttccttggc tttaccttca agcccagact ttgtgcaaaa ccaaacggtg
      901 aaaaattctg gggtttcaga ccggccataa gtagtaagag caggaagaag atcatcggaa
      961 agttgcggga attggcgatt caaaattggg tattctggga tatccaaaag atagccgccg
     1021 cactggcacc gatgatccgg ggatggatcc attactatgg gagataccgt atatcagagt
     1081 tgcaatgggt gttcaggtca ctgaacagcc ggctcgccaa atgggcgagg aagctataca
     1141 aactcaggac gtatgcgaaa tcctatggtt ggttgaagag gatgattaaa tggtacccta
     1201 acacatttgt tcattggacg tatggcttta ccggttgaat ctatctggaa gagccgtatg
     1261 atgggagact gtcaagtacg gttccgtgag aggctggggg tgaaactccc ccggcctact
     1321 cgacttaagt aaacaaatcc tatctttgca ctatgggtgg tttacttact tattgtgtgg
     1381 ctggtcatgt gtttgggatt cagactcctg acaagacggt gactaggggt attctctcca
     1441 gttatgcttc gtttactgtt gaaaacagca cggacagcgg gcatttgttt tattttcagg
     1501 gcaaccgcca ggtggatatt cccgatgttc cctgcgatga cgtagtccaa agggacacag
     1561 tgacttatag tttgtatcat acagtacagg gtataacggc ttccatgcaa caaaacggcc
     1621 gcgagcaccg gctctttgcc tcgcccggct atcaggaaat aatatccgat gtcaccttac
     1681 tttccggtaa tgaagctccg ttcataaaca gtttcctgag gctggcctat ggcgtcaggg
     1741 ctgtccatcg tcaaaccatc aaagtgcacg catctgtcat tgaattgaac ggaaaggcac
     1801 tggtgttcct gggcaaaagc ggagcgggca aaagcacaca cagccgcttg tggctgcagc
     1861 acattccggg ctgtacactg ctgaatgatg acgaacccat actgcggatt tttgaagacg
     1921 gggctgtgag ggtttacggg gcaccctgga gcggttctac cccctgttac agaaacgatt
     1981 gggcagaggt tgccgccatt gtgcatttac accagagccc ggacaataaa ctgagcagaa
     2041 tgacacccct ggaaggattg tcgtcgttat tcaaatctac ggcccttctc cgttcagacc
     2101 gcgaaaacaa agacaaggtg ctggacgtgg tgaccggtgt tgttgagcgc atcccggttt
     2161 accggctcgc ttgccggccc gatcatgaag cggtgttgct gtccaggtca ttattgccta
     2221 aggaatagaa ctgatgatca tacccaacga cattttattc ccgcatatca gggaagagat
     2281 cagggccggg cacagtgtca ggatccagac aagaggcaac agcatgctgc cactgatccg
     2341 tcccggcaca gacgaaattg aactgagccc gctggacaaa aggtccctgc agaaaggaaa
     2401 tatcgtactg gcacgatcgg aagacggaag gtatcttgtt cacagaattg aaaaggtgaa
     2461 gggtgaaatg gtctttatcc gtggtgacgg aaatgcctcg gcaagggaaa cctgcaccgt
     2521 gaacaacttt tttgcccagt tgacggggat cttcaggaag gggaaaaaaa tcgaaaaaaa
     2581 cagtttccaa tggaagttcg cacaatacgg ctggccgtct tcccctttgt tgcggcggat
     2641 ttgtctgggc gtattccgaa ggctccattg tttacaccaa agaataaaaa gcaattgata
     2701 tgcaaatcaa gaagaacatc agaataaaac aaatagggga tgaaaagatc ctggtgtcta
     2761 acgacggcaa aaacctggat tatacgagga taatttccct aaaccaatcc gctgcgtttt
     2821 tgattgaaga aagtatggag aaagaattta ccgttcagga gtgggcgcac cggttgacag
     2881 acaggtacga tattgattat gccctggctt tgtctgatgc acagaaagtc gtggacaaat
     2941 taaagcaggc aggtgtactc tgcgaataag ccattgtgta ctgactgatg cttcagttac
     3001 cgcccgggga tgaccgggtc cgcggggaac cgggttctta tggaagcacc gggttttcag
     3061 gagttcctgc ttcttcccgg agcaccgagt ctgcagggaa gatggtttct cccatcagga
     3121 cctattctgc aggtaaactg gtttctccca gggaaaccga gtctgcaggg aaggccattt
     3181 cttccgggaa atccgttgcc ctgagggtcg gatcaaactc gcccggttta cttggatcct
     3241 ccttcccgtc agcgggatct tcgggcttga gacaactgtt caggcccgtc acgaatagca
     3301 gagagattac cagccatgac aaaaatgccg ttgatagttg tttgagtttt ttcatggtac
     3361 aaatgtaaga aaaaatggaa gaaaatttca cacgtctggg gcagcggttc acgcgactgg
     3421 ggcaacgatt ggcaaggtac ggactggacg ggtttgtcct ggcactgatt actgccattc
     3481 tggtggctta cctttggccc gggcccgggg tgtaccgagg aatggttgat ttgcaggaca
     3541 ttaaccgtgt tgggatagcc atgatcttct ttttctacgg gctaaggctc aatccggcgc
     3601 aactaaggac aggactggca aactggcgcc tgcatgttgt cacacaggcg gttactttca
     3661 tactgtttcc tgcactggtt ttactggtaa aaacattggc cgggggaacc tgggatgcgc
     3721 ccttgtggtc ggggaccttt ttcctggccg ccttgcccac caccatctct tcttccgtgg
     3781 tccttgtttc catgggccgg gggaatgtgc cggcagccat attcaatgcg tctgtatcca
     3841 gcctgctggg gatattcctg acccctttat ggatgggcat ccttcttgtt ggttacaaag
     3901 gcggcttttc tccgacacag gccatcctgc agctctccct gcaggtattg ctgcctgttg
     3961 tcgcggggat gatgctgcac aaaagatacg gtatactcac tgtcaggcat ccacgcactt
     4021 tcagactttt tgaccagggt gtcatcctgc tcattgttta tgtttctttt gccgagtcgt
     4081 ttttcaacaa gatgttcggt ggatattccc cctggcacat cctgctgctt tgtgctgcca
     4141 tgctggcccc tttcatgccc atttccggga acgggcctat cccgaatatc agcagcgggt
     4201 ccaggaccgc attgatgacc gctgaggtga gcatgataat gccaggggtg aaggtatctc
     4261 cggttgcccg gactatgttg ttc
//
LOCUS       DAOSOF010000150         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20692_length_4273_cov_3.860360, whole genome shotgun sequence.
ACCESSION   DAOSOF010000150 DAOSOF010000000
VERSION     DAOSOF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20692_length_4273_cov_3.860360"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLM68_07760"
     CDS             complement(<1..203)
                     /locus_tag="PLM68_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOT55287.1"
                     /translation="MSNYKSKLHNIKAFAFDVDGVLTDGGIISLPDGDLFRTHYSRDG
                     YAIRVAIEYGYPVAIITGGVSPSV"
     gene            260..>1191
                     /locus_tag="PLM68_07765"
     CDS             260..>1191
                     /locus_tag="PLM68_07765"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT55288.1"
                     /translation="MRTFGYLRTVTAILTVLFFGFSAASCDQTGTTTTFAGKVTDSET
                     GQALPGVAVTVMPFSLGVETDQEGTYAIVLPRTTEDVYLVFSKPGYESWETLSMPLSP
                     KKKNLYTIDAQLRSRIAKAVLSSQSVDFGAVDTSVSLTLSNPGGDTLRWGFLEMYFPH
                     WLKVEPSSGELEIGASQAIDFTCDRSGLPIGEQKSEVQLVGGEVPLTVQVRLLVEGAA
                     LVAKGTTFDFGEEDTLATTVLANQGNIPLEWNLKDKLPAWLEWDPAQGTIGAGKEVTV
                     NIIADQSRSEFGTHTFSTDLLSNGGDTTYFFSIA"
     assembly_gap    1192..1201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1202..3103
                     /locus_tag="PLM68_07770"
     CDS             <1202..3103
                     /locus_tag="PLM68_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55289.1"
                     /translation="RTQDLIKVSPQSLDFGTENTALTLTVERLSGIHTVPFTVVCEDE
                     NLQLSLTQGEITKEMPSAGIVVTLDRENIPIGASESQIKVLAPDQEFSIKVNYATAAV
                     PAEVNTEGYELDGSFRLRLKGKLVSNGGGSVTRHGHCWGTNPEPDPDNAYFSDFGPIE
                     EGRTFISYPDALPETGTTWYFRSYAVNEMGTSYGQTMTLTYVPPLLGNPLVTFNETSE
                     ALSLRVNEVSGLPFSDHGFVWNTTGDTPSMDKDNLISLGQKTSGSNFFYNLSKPKRGV
                     TYYIRSYAINDFGLYQSGTSSLYVNIEEPELETGGVGEVSYYEALLKGNLVVKGSEDI
                     LSYGHCWSTSEEPTIENSHSDLGTPQYTGEFTSRATALLINTLYYYRAYVKTSKGVYY
                     GRVEEFTTGRDKQVVVQDGLIMYITTDQTPKAYDWTGRAYDLVVSNGVTYNTNNKPSG
                     TNTTNAAISFNGSSGYLLSSRVNPISGIDRGTINFWLRFRQTMNKSLVYPIFGSVSEG
                     GFYVEIRHNGTDWVLTLCMGPGMETHVITLPAFAGIDISSMLGTSWHMMAIASTGTSM
                     KVYLDGINLYTQSMQMTFGVQKDFVVGANALNSTTINTFLPADMACLRFYNRVLSDPE
                     ISEIFNAGM"
     gene            complement(3105..>4273)
                     /locus_tag="PLM68_07775"
     CDS             complement(3105..>4273)
                     /locus_tag="PLM68_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55290.1"
                     /translation="SLGKRFSVTVISYKNITAERLADPEKVYIPFIASGGCEETFQSY
                     GFSLPLPLFFLYDNQSNSLPATLELSTYVTQGRLSFPIIFSEDEITEELLSRMLRMQQ
                     TFRKLKSTRIALFGDASPWLLSSKVDTNAISRMLKVCFQNIEIPHICKQYMHLTALSR
                     ENKELYNNILKKSTCLKNCTKDDLSDAIRMYQAMDELMVYYSCNALTVKCFDLIKSCQ
                     VTACLAISLFNQAGIPAACEGDIPSLLTMIMAGNLSQSPVFMANPVGYNREQNTLDIA
                     HCTVPMSMTSSFCLNSHYETGVGIAVEGTLEKDVPYTAVKWIGNTLERFFVSEGVAVE
                     SKHSDNQCRTQMTLRLDRPVGDLILNAMANHILVVKGRYADILTTWNGFLSYLR"
BASE COUNT         1150 a          995 c         1029 g         1089 t
ORIGIN      
        1 acggaaggcg acacaccccc cgtgatgatg gccaccgggt atccatattc tatggcaaca
       61 cgaatggcat aaccatctcg tgaataatgt gtcctgaaaa gatccccgtc aggcagggaa
      121 attatacctc catctgttag aacgccatct acatcaaatg caaaagcttt gatattgtga
      181 agtttgcttt tataattgct catagctctg ttttaacatc ggcaaagtta attatatttg
      241 cacatgtaac agtaatggca tgcgtacttt tggttatctg agaaccgtta cagcaattct
      301 gacggtcttg ttttttggat tttctgccgc gtcttgtgac cagacaggca ctactacgac
      361 atttgcagga aaagtgaccg attccgagac cgggcaggct ttgccagggg tagccgtaac
      421 ggtgatgcct ttcagcctgg gtgttgaaac agaccaggaa gggacgtacg ccattgtgtt
      481 gccgcgcacc accgaagacg tataccttgt cttcagcaag cccggttacg aaagctggga
      541 aaccctaagc atgccgttgt cccctaaaaa gaaaaacctg tatacaatcg atgcgcaact
      601 ccgttcccgc attgccaaag cggttctttc atcgcagagc gtggattttg gagcggttga
      661 cacctctgta tcccttaccc tgtcaaatcc tggcggagat accctcaggt ggggtttcct
      721 ggaaatgtat tttccccact ggctcaaagt tgaaccatcg agcggcgagc tggaaatagg
      781 agcgtcacag gctattgatt ttacctgtga ccggagcggc ctacccattg gtgaacagaa
      841 atcagaggta caattggtag gaggtgaagt acctctgact gtacaggtgc gattgcttgt
      901 agagggcgcg gcattggtgg caaaaggaac aacttttgat tttggggagg aagataccct
      961 tgccaccaca gtcctggcaa accaggggaa cataccgctt gaatggaacc tgaaagacaa
     1021 actgcctgcc tggctggaat gggatcctgc acaaggcacc ataggcgcag ggaaagaagt
     1081 tacggtaaat atcattgccg accagtcccg ctcagaattt ggcacccata ctttcagtac
     1141 tgacctgctc tccaacggag gagacaccac atacttcttt tccatagcca gnnnnnnnnn
     1201 naggacacag gaccttataa aggtttcgcc tcaatcactg gactttggaa ctgaaaacac
     1261 tgcgcttacc ctgactgtcg aacgcctgtc tggcatacat acggttcctt tcacggtggt
     1321 gtgcgaggat gaaaacctgc agctttccct cactcaggga gaaataacaa aagaaatgcc
     1381 ctctgcaggg atcgtggtta cgctggaccg tgaaaatatc cctattggtg catcggaaag
     1441 ccagatcaag gttcttgctc ccgaccagga attctccatc aaggtgaatt acgcgacggc
     1501 agctgttccg gcagaagtca atacagaagg gtatgagctg gatggctctt tcaggctccg
     1561 gcttaaagga aaactggtca gcaacggagg aggctcggtg acccggcacg gacactgctg
     1621 gggaacgaat cccgagccag atcctgataa tgcctatttc tccgatttcg ggcctatcga
     1681 ggaaggccgt acctttatat cctaccccga tgcgttacct gaaaccggga caacctggta
     1741 cttccgttcc tatgccgtaa atgaaatggg cacttcatat ggccagacca tgaccctgac
     1801 atatgtaccc cctttgctcg gaaatccttt agttaccttt aacgaaacat ccgaagccct
     1861 ttctctcagg gtgaatgagg tatcaggcct gcccttttca gaccatggtt ttgtatggaa
     1921 tacgacagga gatactcctt ccatggataa ggacaacctt atcagtctgg gtcaaaaaac
     1981 atcgggatcc aatttctttt acaacctgag caagccgaaa aggggagtga catattatat
     2041 acgcagttat gcgattaatg actttgggct ataccagtcc ggaacaagct cattgtatgt
     2101 gaatattgaa gagccggagc ttgaaacggg cggagtaggt gaagtatctt attatgaggc
     2161 ccttttaaaa gggaatcttg tggttaaggg ctccgaggat attctctcat acggacattg
     2221 ctggagtacc tctgaagaac caacaattga gaatagtcat tctgacctgg gaacacctca
     2281 gtacaccggt gaatttacgt cacgggctac cgcattgctt atcaatacat tatattatta
     2341 ccgtgcctat gttaaaactt caaaaggtgt ttattacgga agggtagaag agtttacgac
     2401 cggaagagat aagcaggtgg tagtgcagga cggtctcata atgtacataa ccacggacca
     2461 gacacccaaa gcctatgact ggacggggag ggcttacgac ctggtggtat caaacggtgt
     2521 gacatacaac acaaataaca aaccatccgg aaccaataca accaatgcag ccatttcatt
     2581 caacggcagc agcggttacc tgttgagcag ccgtgtgaat ccgatctctg gaattgacag
     2641 gggaaccatt aatttctggc tccggttccg gcagactatg aacaaatcgc tggtttatcc
     2701 tatctttggg tcggtatctg aaggaggatt ttatgtggaa atccgtcaca atgggaccga
     2761 ttgggtcctg acactttgta tggggccagg catggaaacc catgttatca cgctgcctgc
     2821 ctttgccgga attgatattt cctctatgct gggaaccagc tggcatatga tggcaatcgc
     2881 ttccaccggc acctcaatga aagtatacct ggatggaata aatctctaca cccagtccat
     2941 gcagatgaca tttggcgtac agaaggattt tgtggtagga gctaatgcac tcaacagcac
     3001 aacgatcaat acattcctgc cggccgatat ggcctgcctc aggttttaca accgggtgct
     3061 ttcagatccc gagatttcag agatattcaa tgccggaatg tgatttaccg gaggtaagaa
     3121 agaaaaccat tccaggtagt cagaatatct gcgtatctgc ctttgactac cagtatgtgg
     3181 ttggccattg cattgagaat taaatcacct accggcctgt ccagtcgaag ggtcatctgt
     3241 gtgcggcact gattgtcaga atgttttgat tctactgcaa cgccttcaga tacaaaaaaa
     3301 cgttccaggg tattgccaat ccacttgact gctgtatacg gcacatcctt ttccagggtc
     3361 ccttccacgg caataccgac ccccgtttca taatgagaat tcaaacagaa agacgaggtc
     3421 atggacatag gaactgtaca atgtgcaatg tccagggtat tctgttccct gttgtaacca
     3481 acgggattgg ccataaaaac gggactctgg gataaattac ctgccatgat catggttagc
     3541 agtgagggaa tgtctccttc acaggcagcc ggaatccctg cctggttaaa aagggaaatg
     3601 gccagacagg ctgtgacttg gcatgatttt atcagatcga aacattttac ggttaacgca
     3661 ttgcacgaat agtaaaccat cagttcatcc atggcctggt acatccgtat ggcatcactc
     3721 agatcatctt ttgtgcagtt cttaagacag gtgctttttt tcagaatatt gttgtacaat
     3781 tccttgtttt cacggctcaa cgctgtaagg tgcatgtact gtttacagat atggggtatt
     3841 tcaatatttt gaaagcatac tttcagcata cggctgatgg catttgtatc cactttggaa
     3901 gataaaagcc agggcgatgc gtcgccgaac agcgcgatgc gtgtactctt taactttctg
     3961 aaagtttgct gcatacgcag catcctgctg agcagttctt ctgtaatttc atcttcagaa
     4021 aaaataattg gaaaactcaa cctgccctgt gttacatagg ttgaaagttc taaggtggca
     4081 ggcagggaat tgctctgatt gtcatataga aaaaacaacg gcaggggaag cgaaaagccg
     4141 tagctttgaa aggtctcttc gcagccgccc gatgctatga agggaatata cactttttcc
     4201 ggatcggcca agcgttctgc cgttatgttt ttgtaagata tcacagtgac actgaagcgc
     4261 tttcccagcg act
//
LOCUS       DAOSOF010000151         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_20770_length_4263_cov_4.779943, whole genome shotgun sequence.
ACCESSION   DAOSOF010000151 DAOSOF010000000
VERSION     DAOSOF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20770_length_4263_cov_4.779943"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PLM68_07780"
     CDS             complement(<1..1789)
                     /locus_tag="PLM68_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT55291.1"
                     /translation="MRCLSPKDNNFYICLLQKTTMATYLQVDNLTKSYGDRVLFNKIS
                     FHINQGDKVALVAPNGSGKTSLLDVLAGKESPEGEGTIKFMSGIQVAYLEQDPSYDPE
                     LTVLEQVRSADKKVMQTLTAYKQALESEDKKRLEKAINDMDRLDGWNYDQKIERILTH
                     LQFGAVDRKMGTLSGGEIKKVALAGMLIQEAPFMILDEPTNHLDIEVIEYLEDYMSAT
                     QATLFMVTHDRYFLDRVCNTIYELENGELFTYRGNYSLFLEKREERLANRAAETDKAR
                     NLYRRELQWIRSTPCARTGKSKSRLESFGKLKERIGPAGNTRSMEINAGIARLGTKII
                     HARNLSFTYGEEPLLKDFTYNFSRNEKIGIVGGNGVGKTTFLRLLAGELEPLSGTLQH
                     GTTVRFGFYRQEGISFNPGDTVFDVVHEIAEVVALADGSKVTAASFLSRFLFPPSMHQ
                     VKVERLSGGEKRRLYLLTILMRNPNFLILDEPTNDLDILTLNVLEEYLENFKGCLLIV
                     SHDRYFMDKLADHLFAFEGQGIVQDYPGKCSDYYRSRAAAAPTPAPASRPAAHPGAGA
                     AASRSGGSIPVQTPAPASRPAAHPGAGAAA"
     gene            1793..2386
                     /locus_tag="PLM68_07785"
     CDS             1793..2386
                     /locus_tag="PLM68_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOT55292.1"
                     /translation="MKAISYVRYAALRGVLGIVLGVFLLVNPEAGVRTLVRLLAAILL
                     VGGLFALYFAFKKAPQNDTARTSRGPEPDKGLQRLLYMTSLVFIVFALLLLLFPAFFA
                     GLTMFLTGIIILLSSLTQITGIIQFRKANPVGLPWYIYINPVIILILSLVIMLNPFDS
                     AATLVIFAGIICLVYAVTEIVQAIVQYRLIRKSKTPV"
     gene            2386..3546
                     /locus_tag="PLM68_07790"
     CDS             2386..3546
                     /locus_tag="PLM68_07790"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT55293.1"
                     /translation="MKIVVAMDSFKGSLTSFDAGHAVDRGIEKAWQHMDRTLPEVKVL
                     PLADGGEGTVTALIQGMGGVYRNIKVRGPHKAQVEAIYGIVTVTDGTPTAVIEMASAA
                     GLWLVPEKNRNPLNTTTYGVGQLILHALSKGCRNFLVGIGGSATNDGGIGMLTALGFE
                     FRDSRGRDAGVCGKDLEQIVSIRTAGKHPALDQCTFRVACDVINPLCGPFGAAAVFGP
                     QKGASRDDIRRLDKGLTNLADVAAKMATRYPEKWDNRDVPGAGAAGGLGYAFVQFLNG
                     KLEPGAAMIMDAIGLEDSICDADLVITGEGCMDRQTAMGKAPMEVARLAKKHGKKVMA
                     FCGSIDPSFFDIKEDLFDGFLAVTPPGASLELASEWLQQAVFEHFTSNGAIL"
     gene            3546..>4263
                     /locus_tag="PLM68_07795"
     CDS             3546..>4263
                     /locus_tag="PLM68_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT55294.1"
                     /translation="MMKRLWMPLLLLLLMAADLFLGSVSLAPADVWSALWGNDALDVV
                     YKIVWNFRFPKMVTALLAGMALSVCGVQMQTLFRNPLADPYVMGISSGGGLGVAFFVM
                     GSSLFSIGQRSVPWLFDLGTAAFSWLGALAVMLLVMAASRRLKSNMSLLILGIMIGSA
                     ASALIGLIQYFSDAPALKSYMLWTMGSFGNVTGDRLLIMTALCLSGLLLSVYNIKDLN
                     VLLMGEQYAQNLGISLSRVRN"
BASE COUNT          868 a         1125 c         1203 g         1067 t
ORIGIN      
        1 tagctgcggc accggcacca gggtgcgcag ccgggcggct ggcaggggca ggagtctgga
       61 ccgggatcga acctccggag cggctagctg cggcaccggc accagggtgc gcagccgggc
      121 ggctggcagg ggcaggagtg ggagcggcag cggcgcggga acggtagtag tcactgcact
      181 ttccgggata gtcctgcacg attccctgcc cctcgaatgc aaaaagatgg tcggccagct
      241 tgtccatgaa atacctgtcg tgtgaaacga taagaaggca acctttgaaa ttttccaagt
      301 actcttccag cacattgagc gtgaggatgt ccaggtcgtt ggtgggttcg tccagtatga
      361 gaaaattggg attgcgcatc aggatggtca ataggtagag gcggcgcttt tcaccgccgc
      421 tcaggcgttc caccttcacc tggtgcatgg agggaggaaa caggaaacgg ctcaggaaag
      481 aggctgcggt gactttgctg ccgtcggcca gggccaccac ctcggcaatc tcatgcacca
      541 catcaaatac cgtgtccccc gggttgaagg aaattccttc ctggcggtaa aaaccgaacc
      601 gcacggtcgt tccgtgctgc agggttcccg acagcggctc cagttccccg gccaggagcc
      661 ttaaaaaggt ggtcttcccc accccgtttc ctccaacgat cccgattttc tcgtttcgtg
      721 aaaaattata ggtgaagtct ttcagcaaag gctcctcgcc gtaggtaaag gacaaattcc
      781 tggcatgtat gatcttcgtt cccaggcggg ctatcccggc attgatctcc atggagcggg
      841 tgttgcctgc cggtccgatt ctttctttca gcttgccgaa gctttcaagc cttgatttcg
      901 atttgccggt gcgggcacaa ggtgtgctcc gtatccactg caattcccgg cggtacaggt
      961 tccgtgcctt gtcggtctct gctgcccggt tagccaggcg ctcctcgcgc ttttccagga
     1021 acaggctgta attgcccctg tatgtgaaca gttccccgtt ttccagttcg tagatggtat
     1081 tgcataccct gtccaggaaa taacggtcgt gtgttaccat gaagagggtg gcctgcgtgg
     1141 cagacatgta atcctccagg tattctatga cttcaatgtc caggtggttg gtgggctcgt
     1201 ccagtatcat gaacggggct tcctggatga gcataccggc cagggccact ttcttgatct
     1261 ctccgcccga cagggtcccc attttcctgt ccactgcacc aaactgaagg tgtgtcagga
     1321 tgcgttctat cttctggtcg taattccagc cgtccagccg gtccatgtcg ttgatggcct
     1381 tttccaggcg tttcttgtct tccgattcca aagcctgttt gtaggcagtc aacgtctgca
     1441 tgaccttctt atcggcagag cgcacctgct ccaggaccgt taactcagga tcgtaagagg
     1501 ggtcctgttc caggtaggca acctgtatcc cgctcatgaa ttttatggtt ccttctcctt
     1561 cgggggactc tttgccggcc agcacatcca gcagggaagt cttgccgctg ccgttggggg
     1621 cgacaagggc caccttgtca ccctggttga tgtgaaaaga tatcttgttg aataagaccc
     1681 tgtctccgta tgatttggta aggttgtcaa cctgaaggta tgtggccatg gtggttttct
     1741 gtaacaggca aatgtaaaaa ttattatctt taggcgataa acatctcata gtatgaaagc
     1801 aatatcctat gttcgctatg ctgccctcag gggtgttctg gggattgttc tgggagtttt
     1861 cctgctggta aatcccgagg ccggtgtacg caccctggtc aggcttctgg ctgccattct
     1921 cctggtcggt ggtctttttg ccttgtattt tgcctttaag aaagcccctc agaatgacac
     1981 tgcccggaca agtagagggc ctgagcccga caaggggttg cagcgcctct tgtacatgac
     2041 ttcgctggtt ttcattgttt ttgccttgct gttgctgctc ttccccgctt tttttgccgg
     2101 attgaccatg ttccttaccg ggatcatcat cctgttgtct tccctgacac agatcaccgg
     2161 catcattcag ttccggaagg ccaatcccgt gggattgccc tggtacatct acatcaaccc
     2221 ggtgatcatt ctgatcctga gcctggtcat catgctcaat ccctttgact cggctgcaac
     2281 acttgtcatt tttgccggga tcatctgcct ggtttatgca gtcactgaaa tcgtacaggc
     2341 aattgtccag tatcgcctga tcagaaaaag caaaacaccg gtatagtgaa aatcgtagtc
     2401 gccatggact ccttcaaggg gagcctgaca tcttttgatg caggacatgc cgttgaccgg
     2461 ggcattgaaa aagcctggca gcacatggac cgtactctcc ccgaagtgaa ggtgctcccc
     2521 ctggccgatg gcggagaagg aactgtcact gcccttatcc agggaatggg aggtgtttac
     2581 aggaacatca aggtccgggg gccccataaa gcccaggtgg aagccatcta cggcattgtt
     2641 accgtgacag acggaacacc cacagccgtc attgaaatgg cctctgcagc gggtttatgg
     2701 ctggttcccg aaaaaaacag gaatccattg aatactacaa catacggcgt ggggcaactg
     2761 atattgcatg ccctctccaa gggatgccgc aacttcctgg taggcattgg cggaagcgca
     2821 accaatgacg ggggtatagg catgctgaca gccctgggtt ttgaattccg ggacagcagg
     2881 ggccgtgatg ccggggtgtg cggaaaagat ctggaacaga tcgtttccat ccgcacagca
     2941 gggaaacacc ctgccctgga tcaatgtacc ttccgggttg cctgtgatgt catcaatccc
     3001 ctgtgtggtc cctttggggc cgcagccgtt tttggcccgc agaaaggagc ttcccgtgac
     3061 gatatccgca ggcttgacaa aggccttacc aacctggccg atgtggctgc aaagatggcc
     3121 acacggtatc ccgagaaatg ggacaaccgg gatgtgcccg gagccggggc cgccggtgga
     3181 cttggatatg cctttgttca gtttttgaac gggaagctgg agccgggtgc cgcgatgatc
     3241 atggatgcca taggccttga agattccata tgcgatgcag acctggtcat taccggggaa
     3301 ggatgcatgg accgccagac ggcaatgggg aaagcgccca tggaagtggc ccgcctggca
     3361 aagaaacacg ggaaaaaagt gatggctttt tgcggaagca tagacccctc attcttcgat
     3421 ataaaagagg acctttttga tggtttcctt gccgtgactc cccccggggc ctccctggaa
     3481 ctggcatcgg aatggctgca acaagcggtt tttgaacatt ttacatcaaa cggagctata
     3541 ttgtaatgat gaaaaggcta tggatgcccc ttctcttgtt gctccttatg gcggcggacc
     3601 ttttcctggg ttccgtaagc ctggcccctg cagatgtctg gtcggccctc tggggcaatg
     3661 acgctttgga tgtcgtatac aagatcgtct ggaatttcag gtttcccaag atggtgacag
     3721 ccttgctggc aggcatggcg ttgtccgtat gcggggtgca gatgcagact ttgttcagga
     3781 acccgctggc tgacccttat gtgatgggta tcagctcagg cgggggtctt ggcgttgcct
     3841 tcttcgtgat gggctcttcc cttttcagta tcgggcagcg ttccgttccc tggctgttcg
     3901 acctgggcac ggcagccttt tcctggctgg gagccctggc ggtcatgctg ctggtgatgg
     3961 cggcttccag aaggctgaaa agcaatatga gtctgctcat cctgggtatc atgatcggct
     4021 ctgccgcttc ggccctgata ggcttgatac aatatttttc agatgcccct gccttgaaat
     4081 cgtacatgct ctggaccatg ggcagttttg ggaatgttac aggagaccgg ctgctcatca
     4141 tgaccgcgct ttgcctgtcg ggtttgctgc tctctgtata taacatcaag gacctgaacg
     4201 tgttgctcat gggagaacag tatgcccaaa acctgggcat ctccctgtca agggtcagga
     4261 aca
//
LOCUS       DAOSOF010000152         4214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_21107_length_4214_cov_5.319548, whole genome shotgun sequence.
ACCESSION   DAOSOF010000152 DAOSOF010000000
VERSION     DAOSOF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21107_length_4214_cov_5.319548"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLM68_07800"
     CDS             <1..645
                     /locus_tag="PLM68_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT55295.1"
                     /translation="QGAINGAYLFTPSTYTTKWETPLSLASSDLSNLGIPDDMAPDFT
                     EDDRNQISAHFSTPISAGINVQKEFNKWLSVGVSLTYTLLRGEYQVHTADKSYIVNQN
                     THYLGIPVSIYLRMVNTPHLTVYAVGGGAMDRAIADEYFCQLNDVTTHQYVPVQGFQW
                     SLYGGLGIEYKWSELMGIYIEPAVSHYFDNNQPKSIRTVQPTQFRMELGIRFRI"
     gene            complement(635..1390)
                     /locus_tag="PLM68_07805"
     CDS             complement(635..1390)
                     /locus_tag="PLM68_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT55296.1"
                     /translation="MISVQNINKFFGPQQVLSDISVDFEEGKTNLIIGASGSGKTVLL
                     KAIAGLHTIDSGRIFYDKTDFTGLGEKQRKAIHQSMGMLFQGAALFDFATARENVRFP
                     LDFFTDWSLARKNDRVDFCLNRVNLGDIGDKYPSELSGGMQKRVGIARAIAMNPRYLF
                     CDEPNSGLDPQTAIVIDRLISELTHEFKMTTIINTHDMNSVMAIGEKVVFLYQGKKEW
                     EGSKEGVLQANNKALNDFVFASEMARLLKEPRS"
     gene            complement(1410..2153)
                     /locus_tag="PLM68_07810"
     CDS             complement(1410..2153)
                     /locus_tag="PLM68_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55297.1"
                     /translation="MKKTFELIGEYILLMKRVFTRPEKWKIFWKQCVMETEKLGMTSI
                     SLIGVISIAIGAVLVIQTAYNIENPFIPKMYVGYMVRESLILEFSCTMVALILAGKVG
                     SNISSDIGTMRLTEQIDAMEMMGINSANYLILPKVVSVCLFNPLLMLFSFMIGILGGW
                     LIVAATGMITLSQYATGLRFAFNGYYIVYAITKMVAFSFIIVTVSALYGYSPKENSME
                     VGRASTRSIVAICLLILIVDLLLTQLMLN"
     gene            complement(2153..3382)
                     /locus_tag="PLM68_07815"
     CDS             complement(2153..3382)
                     /locus_tag="PLM68_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55298.1"
                     /translation="MGRKQEKVRFWIPAGILVLLFFLARQMPLYEPAPEDPFTGTYWY
                     NPYGNVTPGGQWLRSNFHAHSNSWKFITNGKDNTVGDIVTTYDSLGYDIIGVSDYQHI
                     TDTRTVVPATGTEATKNRLFIPVYEHGFNVCKTHQGTIGARMVYWNDYVLPQTRSQKQ
                     HILEQLGERSELVVLHHPAWMGGYSKKDVRRLSGYDLFEVLNDFWISEELWDVALSSG
                     KPAMLIAGDDAHNVFKPTDLARDLTMIHAGAQDPLAPEDVYRALKTGAAYGIEVTRSV
                     ARGSVVQKRACLDALPVLQSCRIQGDSLVVVLSGKARQFHFIGQDGALLKSVPAGADM
                     AADAAGSAHTPDNAAGVADSAFYLLQEQDRYVRVKIALPDSSHIYLNPVMRTMEKNKK
                     PPMPPVRKRRLFTIFDT"
     gene            3420..>4214
                     /locus_tag="PLM68_07820"
     CDS             3420..>4214
                     /locus_tag="PLM68_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOT55299.1"
                     /translation="MNKTLQFISGRRGQWKRLAAAFGNGAQKVSPLWMHCASLGEFEQ
                     GRTVLEAFRQRHPDKKILLTFFSPSGYEIRKNTPLADHVFYLPLDTPRNARRFLDLTN
                     PCAAVFIKYEFWYFYLTTLKKRGIPTYLVSARFRPEQLFFKKYGAPFRRMLACFTHIF
                     VQDERSLRLLSALRNPPREITVAGDTRFDSVGCNIPHDKTPPLFLQLVREPVLIAGST
                     WPEDETLLERLAGDIPELQIVLAPHQVCVAHLESIRRLFGRWNPVFF"
BASE COUNT          964 a         1170 c         1081 g          999 t
ORIGIN      
        1 cagggagcca tcaacggtgc ttacctgttc actccttcca cctataccac aaaatgggaa
       61 acaccgctga gccttgccag cagcgacctg tccaacctgg gcatccccga tgatatggcg
      121 cccgacttca cggaagatga caggaaccag atctcggccc atttctccac ccccatctcg
      181 gccggtatca atgtgcaaaa agagttcaac aaatggttgt ccgtgggagt gtccctcacc
      241 tataccctgc tgcgcgggga ataccaggta catacggccg acaaatcata tattgtcaac
      301 cagaacacgc attacctggg aattccggta agcatctacc tgcgtatggt caacacaccc
      361 cacctgactg tctatgccgt cgggggagga gccatggaca gggccattgc cgatgaatac
      421 ttttgccagc tgaacgatgt gaccacacac cagtacgttc cggtgcaggg tttccagtgg
      481 tctttatacg ggggactggg catagaatac aaatggtctg agctgatggg catatacata
      541 gaacctgccg tatcgcatta ctttgacaat aaccagccca agagcatccg gaccgtgcag
      601 cccacccagt tcaggatgga actgggcatc cgcttcagga tctaggttcc ttcaggagcc
      661 gggccatttc cgaggcaaac acaaaatcgt tcagggcttt gttgttggcc tgcaggacac
      721 cttccttgct accttcccat tcttttttcc cctggtagag gaataccact ttttcaccta
      781 tggccatcac cgagttcatg tcatgggtgt tgatgatggt ggtcattttg aattcgtggg
      841 tcagttcact gatcaggcgg tctatcacga tggccgtctg cgggtccagg cccgaattgg
      901 gttcatcaca gaacaggtac cggggattca ttgctatggc acgtgctatc cccaccctct
      961 tttgcatccc gccactcagt tctgacgggt acttgtcccc tatgtcgccc aggttcacac
     1021 ggttcaggca gaaatcaacc ctgtcgtttt tcctggccag ggaccagtcc gtgaaaaagt
     1081 ccagcggaaa acggacattt tcccgtgccg tggcaaagtc aaacagggcg gctccctgga
     1141 agagcatgcc catgctctgg tggatggctt ttcgctgttt ttctcccagc ccggtaaaat
     1201 cagtcttatc gtagaaaatc ctgcccgaat ctatcgtatg caggccggca atggctttga
     1261 gcagcacggt cttgcccgac ccgctcgccc caatgatcag gtttgtcttt ccttcctcaa
     1321 aatccaccga tatgtctgac agcacctgct gcgggccaaa gaatttgttt atgttctgta
     1381 cagatatcat cacttccgga tctaaggggt cagttcagca ttaattgggt caggagcagg
     1441 tcaactatca ggatcagcag gcagatggcc acaatggagc gggtgctggc acggcccact
     1501 tccatggaat tctccttggg agaatacccg taaagggcag aaacggtcac aatgataaag
     1561 ctgaatgcaa ccattttggt aatggcatac acaatatagt acccgttgaa ggcaaaacgc
     1621 aacccggtgg catactggct cagggtgatc attccggtgg cggccactat cagccagccc
     1681 cccaggatgc ctatcatgaa actgaacagc atgagcaggg gattgaacag gcacaccgac
     1741 accactttgg gcaatatcag gtaattggcc gagttgatgc ccatcatttc catggcatct
     1801 atctgctctg tcaaccgcat ggtgccaatg tcggaagaaa tgttggaccc caccttgccg
     1861 gccaggatca gggccaccat ggtacaggag aactccagga tcaggctttc ccgaaccatg
     1921 tatcccacgt acattttggg aatgaacgga ttctcaatgt tgtaggcagt ctggatgaca
     1981 agcacggctc caatggcaat ggaaataacc cctatcaatg aaatggaagt cattcccagt
     2041 ttttcggttt ccatcacgca ctgtttccag aaaatcttcc atttttccgg cctggtgaac
     2101 acccgcttca tgagcaggat gtattcccct atcagttcaa aggttttttt gatcatgtgt
     2161 caaagatagt aaataggcgg cgtttccgga caggaggcat gggtggtttt ttattctttt
     2221 ccatagtgcg catcacagga ttcaggtaga tgtgcgaact atcgggcagg gcgatcttca
     2281 cccgcacgta acggtcctgc tcctgcaaca ggtaaaacgc ggaatccgca acccctgcgg
     2341 cattgtccgg ggtgtgcgcc gagccggctg catctgcggc catgtcggct ccggccggga
     2401 cgcttttgag caaagcgccg tcctggccga taaagtggaa ttgccgtgcc ttcccgctca
     2461 ggaccacaac caggctgtct ccctgtatgc ggcaggattg cagaacgggg agggcgtcca
     2521 ggcaggcgcg cttttgaacc acggaacccc ttgccacgct tcgggtcacc tcaatgccgt
     2581 aggccgcacc tgttttgagt gcccggtaaa catcttccgg agctaaagga tcctgcgccc
     2641 cggcatggat catggtcagg tccctggcaa ggtcggtggg tttgaaaaca ttatgcgcat
     2701 cgtcacctgc aatgagcatg gccggtttgc ccgatgacag cgccacgtcc cacagctctt
     2761 ccgatatcca gaaatcattg agcacctcaa aaagatcgta cccggataga cggcgcacat
     2821 cctttttgct gtagccgccc atccatgcgg gatggtgcag gaccacaagc tccgaacgtt
     2881 cgcccagttg ttcaaggatg tgttgcttct gtgagcgtgt ctggggcagc acatagtcat
     2941 tccagtatac catgcgggcc ccgatggttc cctggtgcgt tttgcataca ttgaaaccat
     3001 gctcatacac aggaatgaac agcctgtttt tcgtggcttc cgttccggta gccggcacta
     3061 ccgtcctggt gtctgtaatg tgttgataat ctgacacccc aatgatgtca taccccagcg
     3121 agtcgtaggt ggtcactatg tccccgacag tattgtcctt cccgtttgtg ataaatttcc
     3181 acgagttgga gtgggcgtga aaattgctcc tgagccactg tccgccgggt gtaacgttcc
     3241 catacggatt ataccagtac gttccggtga aaggatcttc gggagccggt tcatagagcg
     3301 gcatctggcg cgcaaggaaa aaaagcagta caagaattcc tgccggtatc cagaagcgca
     3361 ctttttcctg tttcctgccc atgctgtaaa ggtaggtctt tttagcctat ctttatgcca
     3421 tgaacaagac actgcagttt atcagcggaa ggaggggaca gtggaaaaga cttgcggccg
     3481 ccttcgggaa tggagcacag aaagtgtcgc ccctttggat gcattgcgca tcactgggtg
     3541 aattcgaaca gggaaggacg gtgctggaag cgttcaggca gaggcacccg gataaaaaga
     3601 tcctgcttac ttttttctcc ccatccggct acgagatccg gaaaaacaca cctcttgccg
     3661 atcatgtgtt ttacctaccc ctggacacac cccgcaatgc ccgcaggttc ctggacctga
     3721 caaatccctg cgctgcggta tttataaaat atgaattctg gtatttttac ctgacaaccc
     3781 tgaaaaaaag gggaatcccc acgtacctgg tttcggcccg tttcaggccc gaacagctct
     3841 tcttcaagaa atacggggcc ccgttccgtc gcatgctggc ctgttttaca catattttcg
     3901 tacaggacga acgctccctc cggctcctgt ctgccctaag gaatccccca cgagaaataa
     3961 cggtggcggg cgatacgcgt tttgacagcg tgggctgcaa catcccccat gacaagacac
     4021 cgcccctgtt tcttcagctg gtaagggaac ccgtactgat agccggcagt acctggcctg
     4081 aagatgaaac cctgctggaa cgcctggcag gagatattcc ggagttgcag atcgtcctgg
     4141 ccccgcacca ggtctgtgtt gcacacctgg agtccatccg gcgcctgttc ggccggtgga
     4201 acccggtgtt tttt
//
LOCUS       DAOSOF010000153         4159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_21475_length_4159_cov_5.792154, whole genome shotgun sequence.
ACCESSION   DAOSOF010000153 DAOSOF010000000
VERSION     DAOSOF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21475_length_4159_cov_5.792154"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..446)
                     /locus_tag="PLM68_07825"
     CDS             complement(90..446)
                     /locus_tag="PLM68_07825"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3244 domain-containing protein"
                     /protein_id="HOT55300.1"
                     /translation="MKTFLICFAFFLLAGTAFAQDSTSTAEIVIKIDPLKPKSLIWEK
                     VLAEYDNQYVYVHILNFTGTAIIEIKNAAGVSVAVNYASFAGAATVKIPIENLSSGEY
                     IVYITAAYRYYGAFLL"
     gene            complement(576..1802)
                     /locus_tag="PLM68_07830"
     CDS             complement(576..1802)
                     /locus_tag="PLM68_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55301.1"
                     /translation="MKEAEDVMKVHAIEDPQLQVLLYGYLGDINLRNRNYPEAEVCFE
                     KGLEASKKAGNHRNYVLAYINLFYAKIYVGKTDEAENIVLQINAMDSIPDELIPSVKK
                     VNAFFYQTGPTFKEAIIHSVNDSLKGMYDTDKPGVLFNISQFYMKLNRLDSAVIYGEA
                     CLNAIVDSINLDNYLYYKHLANLYALTGEYKKSSENYKKALESFNTYHTVVNEKRILE
                     LEKKYNIAAAQQALLKEKLKKRAYVIFSVILFAIVIVTIIGLFYWKHTAVQSKQLEIV
                     KQQKIDQLNFFLEAFNNTAGIFPGLLEEIYQLAHKSRQSCPEMYNDLIACLDKVKRDY
                     KSSFSATTQQDFLKEIFNSEGLQIQVSDREKIIYALSESGFTNELIAGLLNVSSDNIR
                     SSISKIKQKVAANCRI"
     gene            2261..3901
                     /locus_tag="PLM68_07835"
     CDS             2261..3901
                     /locus_tag="PLM68_07835"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT55302.1"
                     /translation="MKNILFVLSFLFASLFWVDGSACTVIVAGKKATADGSVLNSHTD
                     CGPDNRIRIIPGQSFNKGTMAPVFWGILDNTRPIDDLGEILGYIPQVERTYTYFHSAY
                     SQINEHQLCIGESTTNQRPELQLTKGEGEQIMTIEQAQVFALQRCKTAREALELMTHL
                     METYGFLPSCGKDPELVTLADPDEVWILEFFAVGKGWKKDSGKPGCIWAAQRLPDDHV
                     AVISNWSVIKEIDLSKPDQYRASSNYMSVAIENGWYDPNGTMPFIWSNAYAGITPREA
                     HTHRQWLFNALYNPGLEELPPRWTSNPFQNLNQYGQTVEPIGMYPFSFKPNKLFTIKD
                     IMDFQRSTFTGTIYDKENDAVWYIPGADGKMVKSPLATPFPNAELQKLLKTNRRRLVS
                     RVDGEYGFCAQLRSDLPDGIGGIYWVFVDNAYTSPYIPFFTGMTKTPAVYMEYHPDHY
                     SDTSVRWAVDVVDNLLNLCYQEAKKDLVRLREPMEEKFFEKNREIEQQYLALYQKNPK
                     AAAELLNRYAQECADTIMQTYRTLRNTLFEKYTNNKLR"
     gene            3905..>4159
                     /locus_tag="PLM68_07840"
     CDS             3905..>4159
                     /locus_tag="PLM68_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOT55303.1"
                     /translation="MSLDRRNFLKAAGGLAALTILPRRVLGGNGFIAPSNQLTKGIIG
                     TGGMGREHIKYEGARLVAVCDVDTKHLEAAVKMAPDRVRTY"
BASE COUNT         1168 a          969 c          892 g         1130 t
ORIGIN      
        1 tatggcttat ttttgctgat tttcaataat ttaaatactt tttaagggcc gactatttat
       61 atgtttaatt tcacttgtga aagatgttct cataacaaga atgccccata atacctatag
      121 gctgcggtga tataaacaat atattcacca gatgataagt tttctatagg aatcttaaca
      181 gttgcagcac cggcgaagga tgcataattc accgctacgg atactccggc agcatttttg
      241 atttcaatga tagctgtacc tgtgaaattc agaatgtgta cataaacata ctgattgtca
      301 tattcagcga gtactttctc ccagataagt gatttaggct tcaatggatc aatcttaata
      361 acaatttctg cagtacttgt actatcttgg gcaaaagcag taccagctaa tagaaaaaag
      421 gcaaagcata taagaaacgt tttcataatc aactttttag taattttata aattaaatca
      481 caaaagtcaa taacggagaa aaaagtttcg gtaaaaacag ggttaatgta aattaattca
      541 gcaagttata tttgaaaagt ttcgcctccc ggcattcaaa tcctgcaatt tgccgcaact
      601 ttttgtttta ttttggagat gctggagcgg atattatctg aagaaacgtt caacaaaccg
      661 gctatcaatt cattggtgaa accactttct gacaatgcat agatgatctt ttcccggtca
      721 ctgacctgta tctgcaggcc ttctgagttg aagatttctt ttaaaaagtc ttgttgtgta
      781 gtggcagaaa aggagctctt atagtctctc ttgactttat caaggcatgc aataagatcg
      841 ttatacattt ccggacagga ttgccggctc ttatgtgcta actggtaaat ctcttcaagt
      901 aaccccggaa aaatacctgc tgtattattg aatgcttcta agaaaaaatt aagttgatct
      961 atcttttgtt gcttaacaat ttcaagctgt ttgctttgaa cagccgtgtg tttccagtag
     1021 aatagcccaa ttattgttac gatgacaatg gcaaaaagaa taacagaaaa aattacataa
     1081 gcccttttct tcaatttttc tttaagtaat gcctgttgag ccgctgcaat attgtatttt
     1141 ttctcaagtt ccaggatccg tttttcatta actaccgtat gataggtgtt aaacgactcc
     1201 agagcttttt tgtaattttc ggaggacttt ttatattctc cggttaatgc atacagattg
     1261 gccaaatgct tataatacag ataattgtcc agatttatgg aatctacaat tgcgttcagg
     1321 caggcttccc catatataac tgcgctatcc aggcggttca atttcatata aaattgagaa
     1381 atattaaata atactcccgg tttatcggtg tcatacattc ctttgagaga atcattgaca
     1441 gagtgtatga tggcttcttt aaaagttgga cctgtctggt aaaagaatgc gtttaccttt
     1501 tttacagatg gtattaattc atcggggata gagtccatag cattaatttg cagcactata
     1561 ttttcagcct cgtctgtttt gcctacatat attttagcgt aaaacaggtt aatataagcc
     1621 aaaacatagt ttctgtgatt cccggccttt ttactagcct ccagcccctt ttcaaaacaa
     1681 acctcagctt caggataatt tctgttccta aggttgatat cccccagata cccatatagc
     1741 aacacctgaa gctgcggatc ttctatggca tggactttca tgacatcttc tgcctctttc
     1801 aaatggaggt atggcagtgt gtccatggaa ttgagcccat aacggacaat ccccaggtag
     1861 atcagggagc gggcaaggtt ttcgggatct ttgccgttcc tgtaccattc cgctgctaat
     1921 gatattatgg aatcgtccgt gaaatccaca taagatttat ccctggctat ggtcagcaac
     1981 aaattgtaat aagactgcac ttcgccggaa tgctcagata catccatttg ttccaaactg
     2041 tccaggacaa cccggggcct gtggctgagc aggctgtcca gattagccag acgagcttgc
     2101 attacgtgct gcctgattgt cgtattacag gaaaccagca gcaacaaacc aagaactatc
     2161 aaccgggaat ttttcatgat tgttttgcta agttaataaa atcagcgaaa ttgtctactt
     2221 ttgttaactt accgacgctc ggtgcattac ctgatttatt atgaaaaata tcctattcgt
     2281 tctttctttt ctttttgctt ccttattttg ggttgatgga tctgcctgca cggtcatagt
     2341 ggcgggcaaa aaagccactg cagacggaag tgttctgaat tcacatacag attgcggtcc
     2401 cgataaccgc attcgcatta tcccgggaca aagctttaac aagggaacca tggcccctgt
     2461 tttttggggt atactggaca atacgcgccc catagatgat cttggggaga tcctgggtta
     2521 cattccccag gtggagcgaa cctacaccta ttttcactcg gcctattccc agatcaatga
     2581 acaccagttg tgcattggcg aaagcaccac caaccagcgg ccggaactgc aactgacaaa
     2641 aggagaaggc gaacagatca tgaccataga acaggcccag gtctttgccc tgcaacgctg
     2701 caaaacggcc cgggaagccc tggaactgat gacacacctg atggaaacct atggttttct
     2761 tccttcctgc ggaaaagatc ccgaactggt caccctggcc gatcccgatg aggtctggat
     2821 actggaattc tttgccgtgg gcaaaggctg gaaaaaggac agcggaaaac cgggttgcat
     2881 ctgggcggca cagcgcctgc cggacgacca cgtggccgtc atttccaatt ggagcgtgat
     2941 caaagagatt gacctttcca aacccgacca gtaccgtgcc tcttccaact atatgagtgt
     3001 ggccatagag aacggctggt acgatcccaa cggcaccatg ccctttatct ggagcaatgc
     3061 ctatgccggg ataaccccgc gcgaagccca cacccaccgc caatggctct tcaatgccct
     3121 gtacaatccc ggccttgagg aactgccccc gcgctggaca agcaatccgt tccagaacct
     3181 gaaccagtac ggtcagacgg ttgaacccat tggcatgtat cccttctctt tcaaacccaa
     3241 caagcttttc accataaaag acatcatgga tttccagcgg agcacattca ccgggaccat
     3301 ttacgacaag gaaaacgatg ccgtatggta tattcccggc gccgatggca aaatggtcaa
     3361 aagcccactg gccacccctt tccccaatgc cgagttgcag aaactgctga aaaccaacag
     3421 gcgccgcctg gtgtcgcgcg ttgacggaga atacgggttc tgcgcacagc tgcgcagtga
     3481 cctgcccgac ggaattggag gcatttactg ggtatttgtg gacaatgcct atacctcgcc
     3541 ctacattcct tttttcaccg gcatgaccaa aacaccggct gtttacatgg aataccatcc
     3601 cgaccactat tccgatacct ctgtgcgatg ggccgtggat gtggtggata acctgctgaa
     3661 cctctgctac caggaagcaa aaaaagacct ggtccggctc cgggagccca tggaagaaaa
     3721 gttctttgaa aagaaccggg aaattgaaca acaatacctg gccttgtacc agaagaatcc
     3781 caaggcagcc gccgaattgc tgaatcggta cgcgcaggaa tgtgccgaca ccatcatgca
     3841 aacatacaga accttgcgga atacgctttt tgaaaaatat acaaacaata aactacgata
     3901 acatatgagc ctggacagaa ggaattttct taaggcagcc ggtggtctgg ctgccctgac
     3961 catcctcccg cggcgtgtcc tgggaggcaa cggtttcatt gcccccagca accagctgac
     4021 caaagggatc attggtaccg gcggtatggg cagggaacat atcaaatacg aaggagcaag
     4081 gctggtagcc gtctgcgatg tggacaccaa acacctggaa gcggctgtga aaatggcccc
     4141 ggaccgggtc aggacctat
//
LOCUS       DAOSOF010000154         4074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_22084_length_4074_cov_4.773576, whole genome shotgun sequence.
ACCESSION   DAOSOF010000154 DAOSOF010000000
VERSION     DAOSOF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22084_length_4074_cov_4.773576"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLM68_07845"
     CDS             complement(<1..617)
                     /locus_tag="PLM68_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT55304.1"
                     /translation="MVADSGYGSEENYEYLDQNTIEGYVKYNMFHMELKKKHIENPFL
                     PGHLYCYMEKDYFICPMGQHMTYIGDNSRKSALGYVSSTRLYQAQKCEGCPLRGQCFK
                     GKSSRVIGVNVKGRQYRDKAKELLTGERGLYHRSRRPIEPEAVFGQIKYDSGFKCFHY
                     RGNRLTKAEFATIAISHNIKKMASLMGLKAKQGVWQSPPQGVSTPG"
     gene            complement(646..966)
                     /locus_tag="PLM68_07850"
     CDS             complement(646..966)
                     /locus_tag="PLM68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT55305.1"
                     /translation="MKRRTQAVVPAVTIPGLLVKTVFYAYLQNVFSGRKMETLLKRDV
                     NFMWLSGMQAPDFNTINLSRKNRLADVIDDLFTQVVQLLVDGQLVSLEAQRLRHVHPP
                     SGYL"
     gene            1723..2805
                     /locus_tag="PLM68_07855"
     CDS             1723..2805
                     /locus_tag="PLM68_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT55306.1"
                     /translation="MNTLVWIGFCQALFASILMFTKNERTVSDKILSAWLVLLAVEFL
                     TAGLDYHFYETPLLSGSHLLSNAAFYLYVRSLTRSGFHLKYRHLAHLLPFLIFKILAY
                     ILNAALMMGDYLVYEEDYMFRFSYAITSLVSWLIYSPLSLTMVYHYRANLLNQTSRIE
                     TNENIRWLMTVTVFYVIYCFLTYIISFVLIIKGDYYSSIPEIINYSLLLFLLYMLSFY
                     GLRQKHIPEAFQISNDKKNNKKPLLDDQTRKEIGKQVLAYFQEKKAYLNPDLNMDILS
                     ADLQLPKHQLTEVLNTELGKNFFRLVNSYRIEAVKKMLAGSRNHYSIEAIGFECGFSN
                     KSSFYKIFKETVGETPSEYKKRISEG"
     gene            2878..>4074
                     /locus_tag="PLM68_07860"
     CDS             2878..>4074
                     /locus_tag="PLM68_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55307.1"
                     /translation="MKRFFFFVILISTALQARAQWNGNTDLAIHGYGRGSVYGGSKAY
                     DLASVFAEFSLQASARKGPAVLVSDIRFSTGMFYNEKDTRFLIRELYAGYKSRKFDVF
                     LGNQIVNWGRTDGFNPTNNINPNDYFFLSSDPADQQLSNFMLKMQYRPLPGIDLELVA
                     IPFYKMSDYRFDLFDMGELGNYLLFGDDMLPERILANGTYAVRANFDFPEFGASVSWF
                     QGYDPYHGFNVVSVNWTSAQPVVRIASTPYRKTAIGADLAIPVGNLMIVRAEAAYNHT
                     KNPDKKMFIPNPDISYVVGIETNVLGCIVIGQYIGKYTFDFFPLSVPVSPNANDPASL
                     VQYAEKYIDYENRLFNRKVFNQQERTNHAVSLTLTRSFAYDVLSFECTGYYNITSKEW
                     FVRPKLS"
BASE COUNT         1066 a          957 c          896 g         1155 t
ORIGIN      
        1 ccgggggtgg acacgccttg cggaggagac tgccacacgc cctgcttcgc tttgagcccc
       61 atcaatgatg ccattttctt tatgttgtgc gatatagcga tagtggcgaa ctcagctttt
      121 gttagtctgt ttccacgata atggaagcat ttgaagccgc tgtcgtactt gatttgtccg
      181 aagaccgctt ccggttctat agggcgcctg cttctgtgat acagccccct ctcgccggtg
      241 agcaactctt tcgctttgtc cctatattgt cggcctttga cattcacgcc aatgacacga
      301 gagctcttcc ccttgaagca ctgtccccgg agaggacacc cttcacattt ttgtgcctga
      361 tagagtcttg tcgaacttac gtatccaagt gctgattttc tggaattatc tccaatataa
      421 gtcatatgct gtcccatcgg acagatgaag taatctttct ccatgtaaca gtacagatgc
      481 cctgggagga acggattctc tatatgcttc tttttcaact ccatgtggaa catattgtat
      541 ttgacatagc cttcgattgt gttctggtca aggtattcat agttttcctc actgccatac
      601 ccggagtcgg ccaccacctc ctggctctgc ctgttgtaac gggcttcaaa ggtatccaga
      661 tggtggatga acgtgccgca atcgctgggc ctccaaggat acaagctggc cgtcgaccag
      721 caactggacc acctgcgtga agaggtcgtc aatcacatct gccagcctgt ttttacggga
      781 gaggttgatg gtattgaaat ccggagcctg catcccgctc agccacatga agttcacatc
      841 tcgctttaga agtgtttcca tctttcggcc ggagaaaacg ttctgcagat aggcatagaa
      901 cactgtcttg accagcagcc cggggatggt aactgctggt accaccgcct gcgtacgtcg
      961 cttcaatctt gctcagatca aggtggtcaa cgattgtgtt tagcacacga acggggtcgt
     1021 tctcagcgat gaagtctccc aaactcggag gaaaaagcag attgtcgttc tgagtgtaag
     1081 atttatagat tacctttgcc attgctttgt gtgacagttc gtaagttatt tttactcaat
     1141 acaaatatac taaaaacatc tcacaaaaac aatagctatt tatctgttat acagaataat
     1201 agctattttt ttactgtaac ttcgttgata caaaaaaaag aaggtgacta tgtgtcaact
     1261 tgacttttta gtcaccctca ccaatttgaa agatctgcct tgaaagacca ccgtgcggtt
     1321 attgaccagt aatgctgctc aacggcaagg tcgctgcatt gcaaagaact gagcgcctcg
     1381 gcgtaccaaa aagcgctctg ttctttgcag tgcaacggct gcctaaaaca agcagataga
     1441 gcctcggcgt accaaaaaag cgctctgttc tttgcagtgc aactgctgcc taaaacaagc
     1501 agatagagcc tcggcgtacc aaaaagcgct ctgttctttg cagtgcaact gctgcctaaa
     1561 acaagcagat agagcctcgg cgtaaaacaa aagctcccgg ttcttcatgg ggcagcaact
     1621 gcccattgca tgcaggagga gcctcggctt acaataaaaa gctcccggct cttgtgttgg
     1681 tggtgactgt cttctgtttt gttatctttg tgtatgcaat acatgaatac actggtctgg
     1741 ataggatttt gccaggcatt gtttgcttcc atactcatgt tcacaaaaaa tgaacggacc
     1801 gtatcggata aaatactgtc cgcatggctt gtattgctgg ccgttgaatt cctgaccgcc
     1861 ggcctggact accactttta tgaaacaccc ttgttatccg gctcgcattt attatccaat
     1921 gcagcttttt acctatatgt caggtccctt acaagatcgg gtttccactt aaaatacaga
     1981 cacctggcgc acttgttgcc gttcttgatc ttcaagatcc tggcctacat cctcaacgct
     2041 gccctgatga tgggggatta tctggtttat gaggaagact atatgttccg tttttcctac
     2101 gctataacaa gcctggtatc atggctaata tacagtccct taagcttgac catggtctat
     2161 cattatcggg ccaatctgct caatcagact tcccgtattg aaaccaacga gaacatcaga
     2221 tggctgatga ctgttactgt tttctacgtg atttattgtt ttctcactta tatcatatca
     2281 tttgtcctga tcatcaaggg agattactat tccagtatcc cggaaataat caattattcc
     2341 cttctgttgt tccttcttta catgctgagt ttttatggtc tcaggcaaaa acacataccg
     2401 gaagcatttc aaatatcaaa tgacaaaaag aacaataaaa aaccccttct ggatgatcag
     2461 accaggaagg aaatcggtaa gcaggtgctg gcatatttcc aggaaaagaa agcttacctg
     2521 aatccggacc tgaacatgga catcctctca gcagatctgc agctccccaa gcaccagctc
     2581 accgaagtgc tgaatacaga actgggtaag aactttttcc ggctggtcaa ttcataccgt
     2641 attgaagcag ttaagaaaat gctggcaggc agcaggaatc attattccat agaggcaatc
     2701 ggtttcgagt gcggattttc caataaatct tcattttata aaatattcaa ggaaactgtg
     2761 ggcgaaacgc cctcggaata caaaaaaaga atttcagaag gatgattgtt gtccatcttt
     2821 taaaaaaatg attgattttc gattcctttt gtcttcattt gcacaaattt tcgatacatg
     2881 aaacgatttt tctttttcgt catactgatt agtacggcat tgcaagcccg ggcccagtgg
     2941 aatgggaata cagacctggc tatccatggt tatgggagag ggtctgttta cggaggcagc
     3001 aaggcttatg atttggcttc cgtgtttgcc gaattttcgc tgcaggcctc ggccaggaaa
     3061 ggccccgccg ttctggtatc tgatatcagg ttcagcacag gcatgttcta taacgaaaaa
     3121 gatacccggt tccttatcag ggaactatat gcagggtata aaagccggaa gtttgatgtt
     3181 ttcctgggaa accagatagt caactggggg cgtactgacg gattcaaccc aacaaacaac
     3241 ataaatccca acgattactt ttttctctca tcggatccgg ccgatcagca actttccaat
     3301 ttcatgttga aaatgcagta caggcctctt ccggggattg atcttgaact tgtcgccata
     3361 ccgttttaca aaatgtcaga ttaccggttt gatctgtttg acatgggaga actggggaat
     3421 tacctgctat tcggggatga tatgctccct gaaaggatcc tggctaacgg gacatatgct
     3481 gtacgtgcca attttgattt tccggaattc ggcgcttccg tttcctggtt ccagggatat
     3541 gacccgtacc atggattcaa tgtggtttca gtgaactgga catcggcaca gcctgtggta
     3601 cgtattgcct ccacacctta caggaaaacc gcgataggcg cagatctggc tatacctgta
     3661 ggaaatctga tgatcgtccg ggccgaggca gcttacaacc atacgaaaaa tccggacaaa
     3721 aaaatgttca ttcccaatcc ggacataagc tatgttgttg gaatagaaac caatgtgttg
     3781 ggttgtattg tcataggcca atacattgga aaatatacgt ttgatttttt cccgttatcc
     3841 gttccggtct ctcccaatgc taatgacccg gcgtcacttg tccaatacgc cgagaagtat
     3901 atagattacg agaacaggtt attcaaccgc aaagtgttca atcagcagga aaggaccaac
     3961 catgccgttt ccctgacctt aaccaggtct tttgcctacg atgtcctgtc ttttgaatgc
     4021 acaggctatt acaatatcac atcaaaagaa tggtttgtaa ggcccaagtt gagc
//
LOCUS       DAOSOF010000155         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_22202_length_4058_cov_4.346740, whole genome shotgun sequence.
ACCESSION   DAOSOF010000155 DAOSOF010000000
VERSION     DAOSOF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22202_length_4058_cov_4.346740"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..554)
                     /locus_tag="PLM68_07865"
     tRNA            complement(468..554)
                     /locus_tag="PLM68_07865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Leu,seq:cag)
     gene            complement(589..1362)
                     /gene="deoC"
                     /locus_tag="PLM68_07870"
     CDS             complement(589..1362)
                     /gene="deoC"
                     /locus_tag="PLM68_07870"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT55308.1"
                     /translation="METLKQCFSFIDLTTLNATDTGSRGRMFAENVNRFSREYPEMPH
                     VAAICVYPALIRAVRETLQVPGVSIAAVGGGFPASQTFTRIKEYECALCVENGADEVD
                     IVISLNYFFSGHYDLVAMEIRNIRQAIGAGTHLKVILETGALKTEENIRKASFLAMEN
                     GADFIKTSTGKMEPAATPFAARIMCQCIADYYEQTHRRVGFKPAGGIVTSDQALEYYG
                     IVEQILGPEWLNPSLFRIGASRLANNLLKDILSQDLAFF"
     gene            complement(1364..1816)
                     /gene="dtd"
                     /locus_tag="PLM68_07875"
     CDS             complement(1364..1816)
                     /gene="dtd"
                     /locus_tag="PLM68_07875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT55309.1"
                     /translation="MRTVIQRVKEASVFIDGKCKASIVEGLLILAGFEASDNKEDLEW
                     MVRKIINLRIFDDPQGVMNLSVMQTGGQLLVVSQFTLHASTRKGNRPSYIKAAGPEIA
                     VPLYESFCSMLEDFSGVAIQTGVFGAEMEVKLNNHGPVTIIIDSKTKE"
     gene            complement(1823..2614)
                     /locus_tag="PLM68_07880"
     CDS             complement(1823..2614)
                     /locus_tag="PLM68_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT55310.1"
                     /translation="MEKLSFTSPVFGPIHSRRLGSSLGINLMPASRKVCNFNCIYCEC
                     GWTPDPLQQAETENSLMPGREIVTRDLEEKLHTLAGQGKAPDAITFSGNGEPTLHPDF
                     SGIVEDTVALRNKWSPGTKVCVLSNSANLDKKGVMEGLMMADLRIMKVDSAIEKTLRL
                     INNPGAGYSLERILSRLKRFGGDFTLQTLFFTGTIGGKEVDNTTEEELAAWYDMVNEL
                     KPRQVMVYTLDRPTPAPGLKKCPAEKMEVIAKVLKEKGIEVIVAG"
     gene            complement(2616..3233)
                     /locus_tag="PLM68_07885"
     CDS             complement(2616..3233)
                     /locus_tag="PLM68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOT55311.1"
                     /translation="MRNALTILCILLLLPACNQGTKRIPANDLEQVLREMFLTDVILE
                     RSETLKILADSTLVYPPILDKYGYTTEQFMATMNYYSTRPTRFKSMLSRLRKSLDEER
                     TLYNEQLTLKEEQRALVERFNNWLRDSVADKHDLVLKQSLKQILPYDPSREQEWSVDR
                     DSLYKAQPLRILAVVDTTSRRDSIPLYLERPKPFKPRGMVTIKLD"
     gene            complement(3230..>4058)
                     /locus_tag="PLM68_07890"
     CDS             complement(3230..>4058)
                     /locus_tag="PLM68_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HOT55312.1"
                     /translation="NEKAISAYTAALRKEYPNATVLAECWFNFPHQVAYWERGPWPSG
                     PTVMDFPLMGEISRAFIEDTNPGWGQGMVRLYNMIANDFVYKNPNELLVFGENHDTNR
                     MYETLGRNPEKLKMAYTFLATTRGIPQIYYGTEVLLAARDSKKLGHGEERAPMLARVL
                     TAEGRDPLEENVFSYISRLLTWRKTSQTVQYGRLLHFLPDYGENMYVYFRILDNECVM
                     VLINNGNEPVAVDWQKYAEGLGKASKGTEVMSGREITVGQSIQVPAQTAIFFTFAGI"
BASE COUNT          969 a         1010 c          975 g         1104 t
ORIGIN      
        1 ctggcggatg tggggaattt ggaggagctg gacaatatca aggagctggc agatgtgggc
       61 tatttggagg aactggtgct gctggtcaat gagctggatt tggatgtgga gggggtgaag
      121 agggagtaat gtcccgggtg tcccaggtgt cccaggctgt ggatgggaca aagcagagaa
      181 gttcagatgg aattatgaat taaatatgcc taacagaaag ttgaccagta ggatgggaca
      241 gaacaataat aagtagataa tccttagaga atggctctac ctatattcta aataattgat
      301 tatctttgtt tcgcaaagca gacatatgag gtctgttttt gtgtgcaatc aagcatcctg
      361 ggactagata tcttgggatg cttttttatt aagtcgggga tatggtcggg gatttttaaa
      421 atacaaaaac cacaagtagt tgatactaac taacctgtgg tttattttgg tgcccggaac
      481 aggactcgaa cctgcacaag attgctctcg ctagtccctg aaactagtgc gtctaccaat
      541 tccgccatcc gggcgtttct tctcagggct tgcaaagata gacaaaaact aaaaaaacgc
      601 aaggtcttgc gataagatat ctttcaataa attgttggca agacggcttg cccctatccg
      661 gaacagagaa gggttcaacc attcaggtcc caggatctgt tcaacaatac cgtaatattc
      721 aagtgcctgg tctgatgtta cgataccacc ggccggcttg aatcctaccc tcctgtgtgt
      781 ctgctcgtaa tagtcggcta tgcactggca catgatgcgg gcagcaaaag gggtagcggc
      841 aggttccatc ttgccagtgg aagtcttgat gaaatcggcc ccgttctcca tggccagaaa
      901 tgatgctttg cggatatttt cttctgtttt gagggctcca gtttcaagga tgaccttcag
      961 atgtgttccc gcgcctatag cctggcgaat attccttatc tccatggcaa caaggtcata
     1021 atgtccggaa aagaaataat tcagcgatat gacaatatcc acttcgtccg ctccattttc
     1081 tacacaaaga gcgcattcat attccttgat gcgcgtaaaa gtctgcgatg ccggaaaacc
     1141 gcctcccaca gcagctatgg aaacacccgg cacttgcaag gtttccctga cagcccggat
     1201 caatgcagga taaacacaaa tggcagccac atggggcatt tcgggatatt ccctggaaaa
     1261 acgattcaca ttctctgcaa acatccggcc ccttgacccg gtatcagtgg cgttcagggt
     1321 ggtcaggtca ataaagctga aacattgttt tagcgtttcc atgttattct tttgttttac
     1381 tgtctatgat tatggtaacg gggccgtggt tgttcagttt cacttccatt tctgcaccaa
     1441 agacccccgt ctgtatggct accccggaaa aatcctccag catgctgcaa aaagattcat
     1501 acaggggcac tgcaatttcc ggtcctgccg ccttgatgta ggacgggcgg ttccctttcc
     1561 tggtagatgc atgcagggta aactggctta ccaccagcaa ctggcctccg gtctgcatca
     1621 cagaaaggtt catcacgccc tgcgggtcgt caaagatccg caaattgatg atcttgcgta
     1681 ccatccattc cagatcttcc ttgttgtctg atgcttcaaa accggcaagt atcagcaagc
     1741 cctcgactat ggaagccttg catttcccgt caataaagac cgaggcttcc tttacccgtt
     1801 ggatgactgt cctcatgatt ctttatcctg cgacgatgac ttctattccc ttctctttca
     1861 acactttggc aataacttcc attttttctg ccgggcactt cttcaaaccg ggagccggtg
     1921 tcggcctgtc cagcgtataa accatcacct gccggggttt cagctcattt accatatcat
     1981 accatgctgc cagctcctct tccgttgtat tgtctacctc ctttccacct atggttcctg
     2041 tgaaaaacaa cgtctgcagg gtaaaatccc ctccgaaccg cttcaaccgg gacaaaatcc
     2101 gttccaggga atagcctgct ccgggattgt ttatgagccg gagcgttttt tcaatggccg
     2161 agtcaacctt catgattctc agatctgcca tcatcaaccc ttccattaca ccttttttgt
     2221 ccaggtttgc cgagttggac aacacacaaa cctttgtgcc cggactccat ttgtttctca
     2281 gggccacggt atcctcaacg atccctgaga aatcgggatg caaggtgggc tctccgttcc
     2341 ccgagaatgt gatggcatcg ggagctttcc cctggccggc cagcgtatgc agcttttctt
     2401 ccaggtcacg ggtaacaatt tcgcgcccgg gcatcaggga attctctgtt tctgcctgct
     2461 gcaggggatc cggagtccat ccgcattcac aataaatgca attgaaattg cacaccttcc
     2521 gggatgctgg catcaggttg atccccaggc tggagccaag gcgcctgctg tgtatcggac
     2581 caaaaacagg actggtgaat gacaattttt ccatatcaat ccaactttat ggttaccatg
     2641 cctctcggct tgaagggctt gggacgctcc aggtaaagcg ggatggaatc gcgacgggaa
     2701 gtggtgtcaa caactgcaag gatacgcagc ggttgtgctt tgtacaaaga atccctgtcc
     2761 acgctccatt cttgttcccg ggagggatcg taaggcagga tctgcttaag ggactgtttc
     2821 aacaccagat cgtgtttgtc tgctacagaa tccctgagcc agttgttgaa acgctcaaca
     2881 agagcccttt gttcttcttt aagggtcaac tgctcattat agagggtcct ttcttcatca
     2941 agggacttgc gcaacctgct gagcatggat ttgaaccgtg ttggccttgt tgaataataa
     3001 ttcatggtgg ccatgaactg ctcagttgta taaccgtact tatccaggat gggcggatag
     3061 accaaggtgg aatcggccag aattttcaac gtctcgctgc gttccaggat cacgtctgtc
     3121 agaaacatct cgcggagtac ctgttccaaa tcattggcag gtatccgttt cgtcccctgg
     3181 ttacaggcag gtaataataa caatatgcat agtattgtca gtgcattcct catatacctg
     3241 caaaggtaaa gaaaattgcg gtctgagccg gtacctgtat tgattgcccg accgtaattt
     3301 cccttccact cattacttct gtgcctttgg aagccttgcc caggccttca gcatacttct
     3361 gccagtcaac cgcgaccggt tcgtttccgt tgtttatcag gaccatcaca cactcattgt
     3421 ccagtatcct gaaataaaca tacatgttct ccccgtaatc gggcaggaag tgaagcaacc
     3481 ggccgtattg cacggtctgc gaggtttttc tccaggtcag cagccttgat atgtaactga
     3541 atacattttc ttccagggga tcgcggcctt ctgctgtcag tacccgggcg agcatggggg
     3601 ccctttcttc cccgtggccc agctttttgc tgtcacgggc ggccagaaga acctctgtcc
     3661 cataatatat ctggggaatt ccacgggtgg tggcaagaaa agtatatgcc attttcaatt
     3721 tttccgggtt gcggcccaga gtttcataca tgcggtttgt gtcatgattt tctccaaaaa
     3781 ccagcaattc gttgggattt ttgtatacaa aatcgtttgc gatcatattg taaaggcgga
     3841 ccatgccctg tccccagccg ggattggtgt cttctatgaa agcacgtgaa atttctccca
     3901 taagggggaa atccatcacc gtaggaccgc tgggccatgg tcccctttcc cagtaagcaa
     3961 cctggtgggg aaagttgaac cagcattctg ccagaacggt agcgttggga tattcctttc
     4021 tcagggcagc agtataggcg gaaatggctt tctcgttt
//
LOCUS       DAOSOF010000156         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_22774_length_3985_cov_5.212977, whole genome shotgun sequence.
ACCESSION   DAOSOF010000156 DAOSOF010000000
VERSION     DAOSOF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22774_length_3985_cov_5.212977"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1575
                     /locus_tag="PLM68_07895"
     CDS             94..1575
                     /locus_tag="PLM68_07895"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT55313.1"
                     /translation="MKKRNIIILSSVAVAAIVLVIILATGNKDGYTPLVVKAQKGLFE
                     IVVATTGELEAENNIKIEGPSGMTARNVRIGEVKIQDLVAEGTVVRQGDYVATLDRTS
                     ADNSLKDLEDEYEALVAKYDRTLLDTSLTLKALRDDIENQKFNIEEAEIAVEQSIYEP
                     PATQRQYRNNLEKALRSYEQALNNYDLKVEQAQASVNDVSIELNRKKRQLEELRSVLK
                     DFTIYAPSDGMVIYYRDWSGDKRRIGSSISGWDLVVATLPDLSNMLSKTYVNEIDIAK
                     VKPGQEVRIGVDAFPEKSYTGYVKEVSNVGEQLKNADAKVFEVVIKVDGQDEILRPAM
                     TTSNQIVTSSMDSAIFIPSEAIFERDSITYVYTTDGIRQIVVKGESNENFTVIEQGLA
                     EGDELYLSLPMNPSRFKVQGEEYIAVIKQRIIEKAEAEKRRAEEQESVQQKRMMRVNG
                     QGQQNFTLPEGVTLPQGVRPPQGGTPPSGGQRRVVMQPSGTQK"
     gene            1588..3042
                     /locus_tag="PLM68_07900"
     CDS             1588..3042
                     /locus_tag="PLM68_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55314.1"
                     /translation="MNIFFIHRYMHDIHIAVESIVHNKLKSFLTALGIVFGVGAVISM
                     MAIGNGAQQEILDQIEMVGVNNIVILPATVEEVDEESTGTSASQTKKFSPGLQLSDAL
                     AIKQVLPSVKHVSPEVSVFTYITYNGIRKPAKVLGITSEYFQLYNMGLEAGEYFSIEQ
                     ELNSMAVCLIGHNVKIRFFQQNDPIGQFIKFGNNWVKVIGVLEQSTSGASTPKEEAES
                     SGTASTARSGSLGTIGSESMGMQGYDDQVFIPIRTMLLRQENRALAITNPASASGGSV
                     VMAGGGGRRHIVISSNTSTSAGNTNYHQLDRITVQVNDTDELTSSQEVITRILKRRHQ
                     DVPDFEVTVPELLLKQQQRTKDIFNIVLGAIAGISLLVGGIGIMNIMFASVMERTKEI
                     GTRLAIGAKKADVIAQFLSEAILISVAGGVIGVFVGVLLSFLIRSFFDITTIISFSSI
                     VISFGVSAAVGVIFGYSPAKRAAERDPIESLRYE"
     gene            3035..>3985
                     /locus_tag="PLM68_07905"
     CDS             3035..>3985
                     /locus_tag="PLM68_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT55315.1"
                     /translation="MNKIMKINTWFLTCIICMLFAAPGIWAQTNQETADPDSQEYILY
                     SYNLTLEDVLHLAATQSNEALRARNSFQVGYWQYRSYRANFLPSLSFTSQLPEFSRKI
                     VDVPSIDPATGEVRHDYSSEFYNVFSGGLSLQQNLPTGGSVTISSSLQRVDRFSGSFN
                     NHQSTEYLSTALNVRLRQSIFGVNEMKWDRKIEPLKYEEARRTYIENMENVNQRAIQY
                     FFNMAIAQQRLAIAEFNHANNDTLYNIAAGRYELGTIGENDLLQSELAYQNALSTLNE
                     DRLNLEDAENRLRSYLGFNERVKLNIVIPENVPDVIMDVEQ"
BASE COUNT         1148 a          921 c          998 g          918 t
ORIGIN      
        1 cggcaaaatt tttattttat taaactattt tcgtccgagt ccatcaaatg gatacatttg
       61 tcaaatttta gaaatcagac tatataaatc agtatgaaaa agaggaatat catcatcttg
      121 agctctgtgg ccgtagccgc tattgtcctg gtcataattc tggcgaccgg taataaggac
      181 ggttacacac ccctggtagt caaggcacaa aaaggtcttt tcgagattgt tgtagcaaca
      241 accggagagc tggaagcaga gaataacatt aaaattgaag gtccgtccgg gatgaccgct
      301 cgtaacgtcc gtataggcga agtgaagatc caggacctgg ttgcggaggg aactgttgtc
      361 aggcagggcg attatgtggc tacactggac aggacatcag ccgataattc acttaaggac
      421 ctggaagatg aatacgaagc gctggtagca aaatatgata ggacactgct tgatacgtcc
      481 ctgaccctga aagccttgcg cgatgatata gaaaatcaga aatttaatat tgaagaggcc
      541 gaaatagccg tggaacaatc catttacgaa ccaccagcaa cacaaaggca atatcgcaat
      601 aacctggaaa aagcgttgcg gtcctatgag caggccctca acaattatga tctgaaagtt
      661 gaacaggccc aggccagcgt aaacgacgta tccatagaac tgaacaggaa aaagaggcaa
      721 ttggaagaac ttcgttcagt gctcaaggat tttaccattt atgccccaag cgacgggatg
      781 gttatttatt acagggactg gagcggagat aagcggcgta ttggttcatc aataagcgga
      841 tgggacctgg tggttgccac tctccccgat ctgagcaata tgttatccaa aacatacgtc
      901 aacgagatcg atatcgccaa ggtaaaaccc ggacaagaag ttcgcatcgg ggtagatgct
      961 tttcccgaaa aaagctacac gggatatgtt aaagaagtgt cgaatgtggg ggaacaacta
     1021 aagaatgcag acgccaaggt ttttgaagta gtaatcaaag tagatggcca ggatgagatc
     1081 ctgcgccctg ccatgaccac cagcaaccag attgtcacat cttccatgga tagcgccatt
     1141 ttcattccgt cggaagcaat atttgaaagg gacagcatca cttatgtgta tacaacggac
     1201 gggatacgcc agatagttgt caagggagaa tccaacgaga atttcacagt gatagaacag
     1261 gggctggccg aaggggatga actgtacctt tccctgccta tgaatccttc ccgctttaaa
     1321 gtccagggag aagagtacat cgctgtcatc aagcaaagga tcattgaaaa ggccgaggcg
     1381 gagaagagga gagcagagga acaggaatca gtgcagcaaa aacgcatgat gcgcgtcaac
     1441 ggtcagggac agcagaattt taccctgccc gaaggagtta ccctgccgca gggggtaaga
     1501 ccaccacaag gaggaacccc tccttccggc ggtcaacgca gggtggtaat gcagccttca
     1561 ggtactcaaa agtaagcatc cgttcttatg aatatttttt tcatccacag atacatgcac
     1621 gatatccaca tagccgtgga atccatcgtg cacaataagc tgaagagttt ccttacggct
     1681 ctggggatcg tatttggtgt gggggctgtg atcagcatga tggccatagg gaacggagcc
     1741 cagcaggaaa tactggatca gatagaaatg gttggggtaa acaatatagt tatccttcct
     1801 gccaccgttg aagaagttga cgaggagtca acaggcactt cggccagtca gacaaaaaaa
     1861 ttctctcccg gattgcagct ttccgatgca ttggccataa agcaggtgct cccttctgta
     1921 aaacacgtat cccccgaagt aagtgtcttc acctatatca cctataacgg gatcaggaag
     1981 ccggcaaaag tgcttggcat aacaagcgag tattttcaat tgtacaacat ggggttggaa
     2041 gccggagagt atttttccat tgaacaggag ctcaattcca tggcggtgtg cctgatagga
     2101 cataatgtaa aaatccgttt ctttcaacag aatgacccca taggccagtt cattaaattc
     2161 ggaaataact gggtaaaggt gataggtgtt ttggaacaga gcacttcggg tgcttccacg
     2221 cctaaggaag aggctgaatc atccgggaca gcatctactg ccaggtcagg atccctgggt
     2281 actatcggct ctgaaagcat gggcatgcaa ggatacgacg accaggtatt catcccaata
     2341 aggaccatgt tgctccgcca ggaaaacagg gcgctggcaa taaccaatcc cgcatcagca
     2401 tcaggaggtt cagtggttat ggcaggaggc ggaggtcgca ggcatatagt gatttccagc
     2461 aacacgtcga cttcagccgg caatacaaat tatcaccagc tggacaggat cactgtacag
     2521 gtaaacgata ctgacgaact gacctcgtct caggaagtca ttaccaggat cctgaaaaga
     2581 cgccaccagg atgttcctga ctttgaggtt actgtgcccg agctgctgct caagcagcaa
     2641 caacgcacca aggatatttt caacattgtg ctgggagcca tcgccgggat ctcgctcctg
     2701 gtgggaggca tcggcatcat gaacatcatg ttcgcttcgg tcatggaacg gacaaaggaa
     2761 atcggcacaa ggctggccat tggcgcaaaa aaagccgatg tgatagccca gttcctctcg
     2821 gaagccatcc tgataagcgt ggccggaggt gtgattggcg tgtttgtggg agtcctgttg
     2881 tcctttctga tccggtcttt ctttgatatc accaccatca tttcattttc atccattgtc
     2941 atttcattcg gcgtatcggc tgctgtaggt gtgatattcg gttactcccc tgccaaacgc
     3001 gcagctgaac gcgatcccat agaatctctc agatatgaat aaaattatga agatcaatac
     3061 ctggtttctg acatgcatca tctgcatgct tttcgctgcc ccgggcatat gggcacaaac
     3121 caatcaggaa acggcagatc cggattcgca ggagtacatc ctgtattcgt ataacctgac
     3181 actggaggat gtactgcacc ttgccgccac acagtccaat gaagccctga gggcgaggaa
     3241 cagcttccag gtaggatact ggcaataccg gtcgtacagg gcaaattttt taccctccct
     3301 gtcgtttact tctcaattgc cggaattttc ccgcaagata gtggacgttc ccagcataga
     3361 tcctgcaacg ggagaagtgc ggcatgatta ttcctcggaa ttctacaatg ttttcagcgg
     3421 cggtctgtca ctccagcaaa acctgcctac gggaggttcg gtgaccatca gttcatcgct
     3481 gcagcgggtt gaccgtttca gcggatcttt caacaaccac cagagcacgg aatatctttc
     3541 cactgcacta aatgtcaggc tcaggcaatc cattttcggc gttaatgaaa tgaagtggga
     3601 ccgcaagata gagcccctta aatatgaaga agccaggcgc acctacatag aaaacatgga
     3661 aaatgtgaac cagagggcta tccagtattt tttcaatatg gccatcgcac agcaacggct
     3721 tgccattgcc gaattcaacc atgccaacaa cgacacgctg tacaatattg cagcaggaag
     3781 gtatgaactg ggcaccattg gagaaaacga cctgctccag tccgagctgg cctatcagaa
     3841 tgcactctcg accctgaatg aagacaggct gaaccttgaa gatgcagaaa accgtttgcg
     3901 ttcctatctt ggattcaatg agcgtgtgaa actgaatatt gtcataccgg aaaacgttcc
     3961 ggatgttatc atggatgtgg aacag
//
LOCUS       DAOSOF010000157         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_22826_length_3978_cov_4.059648, whole genome shotgun sequence.
ACCESSION   DAOSOF010000157 DAOSOF010000000
VERSION     DAOSOF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22826_length_3978_cov_4.059648"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLM68_07910"
     CDS             complement(<1..854)
                     /locus_tag="PLM68_07910"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOT55316.1"
                     /translation="MKGLFRMLATAVVAILLIVSCTKDPYITPGPGAGNSISLGSAAF
                     SAEYVFSTNYDWTARSSGPWLTVSPSSGMPGDNIILKLSAGENANYDNRTATVTITAG
                     EITRTFTVDQAQNEGFELTKTDYSVGKDGGNITIPISANVTYTCTVSESARSWLAVTQ
                     TKALSNYNIVLTVSKNETYGSRTGTVTIAYGNQESVITITQSENEGFEVTTTDYSVGK
                     DGGNITIPVRANVTYTCTVSESAKSWLSVIQTKALSNYNVVLSASKNETYDSRTGTVT
                     IAYGNQES"
     gene            complement(951..1658)
                     /locus_tag="PLM68_07915"
     CDS             complement(951..1658)
                     /locus_tag="PLM68_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOT55317.1"
                     /translation="MGLLHLFTGLFFIVLGFLVRAFPDLIAGYNTMPPERKKHIDMDG
                     LSRFIRNGLIIMGLAMMVGYLLFNWAGWTFMANMVLIIVVFVGAAVLMLASARFDHYP
                     GKKSKSQYIIFGIIVIIITSVLFMGFMTSRTQFSDDTIRFTGMFATKMKISDIEKVEL
                     TDTIPAIRFKNNGFSLRTVHKGIFTLKEFGRCRLYINSSKGQYLIITEKTGFRTILRY
                     KDNNESQAIYERIEEML"
     gene            complement(1668..2537)
                     /locus_tag="PLM68_07920"
     CDS             complement(1668..2537)
                     /locus_tag="PLM68_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOT55318.1"
                     /translation="MASPLQAQEKTTVSHLTVFDITTNQQTVIKTFPYLIEAPNWTPD
                     GRWLVYNSGGKLYKISPDGQDGPYEISTGTITRCNNDHVISADGKQISLSSSTSRDWR
                     SRIYTVPFEGGEPRLVTPSGPSYLHGWSPDLGTLAYCAERNGEYDIYIIPVSGGEEIR
                     LTSAPGLDDGPEYSPCGVYIWFNSVRSGLMQLWRMRADGSEQEQMTFHTQLNSWFPHI
                     STDGEQVVYIAYHVGDLQPHEHLPNKNVELRLMASSGGPSRLLTKLFGGQGTINVNSW
                     APDSQRFAFVFFP"
     gene            complement(2590..3513)
                     /locus_tag="PLM68_07925"
     CDS             complement(2590..3513)
                     /locus_tag="PLM68_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT55319.1"
                     /translation="MKGETIAIGVAVLWTLSALFFEYAGKRIGSINLNLIRLVCAFLM
                     LGFTMLLINGSFIPVGADKATWFWMSLSGLVGFVLGDYFLFASYTIIMARFSQLIMTL
                     APPFAAIFGFILLGEKMSWISVAGMLVTITGISISILKKQNGTQNRRFQMQLPVKGVI
                     FALMGALGQGVGIVLSKQGMLAYQEVYTAGNALYIPLAATQIRTIVGMISFALIILLK
                     GDLKRFLLVFKDKKAIRSVVSGSVFGPYLGVTFSLMAVQHANTAVASTIMATVPILIL
                     LPEYLFLKRKVTWQQVAGAVLSVGGVTLFFI"
     gene            complement(3544..>3978)
                     /locus_tag="PLM68_07930"
     CDS             complement(3544..>3978)
                     /locus_tag="PLM68_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55320.1"
                     /translation="SDQDALSARTGALEELSVREFGFNDQWDPINSADRSKPYFYWWL
                     RYGSEESVGYVFEKPEKVGSVEVYWLDFDHYDGNYRVPESWKLYYEKDNRWFPVEALT
                     EYTVAKDRYNKLEFNPLITRALKLSVKLQEGASGGIIEWKVK"
BASE COUNT         1050 a          952 c          927 g         1049 t
ORIGIN      
        1 attgattcct gattcccata ggcaatggtt actgtacctg ttcttgaatc atatgtttca
       61 ttttttgaag ccgaaagaac aacattgtaa tttgataatg ctttagtctg gataaccgaa
      121 agccatgatt tggcactttc tgaaactgtg catgtatagg taacattggc tcttaccgga
      181 atagtaatat tgcctccgtc tttgcctacc gaatagtcgg ttgtggtaac ctcaaaaccc
      241 tcattctcag actgcgttat ggtaataact gattcctgat tcccataggc aatggttact
      301 gtacctgttc ttgaaccata tgtttcattt tttgaaacgg taagaacaat attataattt
      361 gataatgctt ttgtctgggt aaccgccagc catgatcttg cactctccga tactgtgcat
      421 gtataagtaa cattggcact tatgggaata gtaatattgc ctccgtcttt tcctaccgaa
      481 tagtcagttt tggtaagctc aaaaccctca ttctgagcct gatcaacagt aaatgtacgg
      541 gttatctcac ctgccgttat ggttacagtt gcagtcctgt tatcataatt ggcattctct
      601 ccggctgaca acttcaggat gatgttatct ccaggcatac cggaggaggg ggatactgtc
      661 agccagggtc cggaagacct ggcagtccag tcataattgg tactgaatac gtattctgct
      721 gaaaaagcag ccgaccctaa cgagatggaa ttcccggcac ctgggccggg ggtgatatac
      781 gggtcttttg tgcaggatac aatgagtaaa atagcaacaa ctgctgttgc cagcattcgg
      841 aacaaacctt tcatagtttt tggattttta gattcttatt acaaaagcca aatataacac
      901 ctttccccta tataaacatt acttttttca gggttttctt aaaaggtttt ctacagcatc
      961 tcctctatcc tctcgtaaat tgcctggctt tcattgttat ccttgtatct gagaatggtt
     1021 ctgaaacctg ttttttcggt aataatcagg tactgccctt tgcttgaatt gatatacagg
     1081 cggcacctgc cgaattcctt taacgtaaag atccccttgt gcactgttct caggctgaaa
     1141 ccgttatttt tgaaacggat ggcgggaatg gtatctgtca gttctacctt ttcaatatca
     1201 gaaatcttca ttttcgtggc aaacatgcca gtaaaacgaa tggtatcatc gctgaactgt
     1261 gtcctggaag tcatgaaacc catgaacaac acgcttgtta taattatgac aatgatcccg
     1321 aaaatgatat actgggattt gcttttctta ccgggatagt ggtcgaacct ggcagaagcc
     1381 agcatgagaa cagcggcgcc tacaaaaacc actatgatga gcaccatatt ggccataaag
     1441 gtccagcccg cccaattgaa caaaaggtag ccgaccatca tggcaaggcc catgatgata
     1501 agaccattac gaatgaacct cgacaatccg tccatgtcaa tatgcttctt cctttccggc
     1561 ggcatggtat tgtatccggc aatcagatcg gggaaagccc ggaccagaaa gcccagcact
     1621 ataaaaaaca atccggtaaa taagtgcaac aatcccatgt tataaagtta gggaaaaaag
     1681 acgaatgcaa aacgctggct gtccggtgcc caggagttta cattgatggt accctgaccg
     1741 ccaaagagtt tggtcagcag gcgtgatggg ccgccggatg aggccatcag gcgaagctcc
     1801 acattcttgt ttggcagatg ctcatggggc tgcagatcgc ccacatgata agcgatatat
     1861 accacttgct ccccgtccgt ggaaatatgc gggaaccagg agttgagctg cgtgtggaag
     1921 gtcatttgtt cctgttcaga gccgtctgcg cgcatgcgcc agagctgcat cagtccggag
     1981 cggaccgagt tgaaccagat gtacactccg cacggggaat attcaggacc gtcatccagg
     2041 ccgggagcgc tggtaaggcg gatctcctcc cctcctgaca ccggtatgat gtagatatcg
     2101 tattcgccgt tcctctcggc acagtaggcc aacgtaccca gatcggggct ccatccatgc
     2161 aggtaactgg ggccggaggg ggttaccagc ctcggctcgc ctccttcaaa aggcacggtg
     2221 taaatgcggg aacgccagtc acgggaagtg ctgctgctca gagaaatctg ctttccatcg
     2281 gcagatatca catggtcgtt gttgcaacgg gtgattgtcc cggtggaaat ttcataagga
     2341 ccatcctgcc cgtcgggaga tattttgtac agtttgccgc ccgagttgta taccagccag
     2401 cggccgtcgg gagtccagtt gggcgcttct atcaggtacg ggaaggtttt gatcaccgtc
     2461 tgctgattgg tggttatatc gaagacggtc aggtggctta ccgttgtttt ttcctgtgcc
     2521 tgtaaaggtg atgccataaa taataaacca agaagaatga ttgaatattt catgatcggt
     2581 taagcgttat cagataaaaa acaaggtcac tcccccaacg ctcaatacgg caccggccac
     2641 ctgctgccag gtaactttgc gttttaggaa aaggtattcc ggcaacagga tcaatatggg
     2701 aacggttgcc atgatggtgg atgccacggc tgtgttggca tgctgtacgg ccataaggga
     2761 aaaagtcact cccaggtagg gtccgaagac cgagccgctg accacagacc tgatagcttt
     2821 cttgtccttg aatacaagca ggaaacgttt caggtctcct ttcagcagga tgatcagggc
     2881 aaaggatatc atccctacga tggtcctgat ctgtgtcgca gccaggggaa tgtaaagggc
     2941 attccccgct gtgtatacct cctggtaagc gagcatgcct tgcttgctca acactatgcc
     3001 tactccctgc cccagggcac ccatcagggc aaaaatcacc ccttttaccg gcagttgcat
     3061 ctggaaccgg cggttctggg ttccattctg ctttttaagt attgatatgg atatcccggt
     3121 aatagtcact aacatgcctg ccactgaaat ccaggacatt ttctctccca gcagtatgaa
     3181 tccgaagatg gcggcaaaag gaggagcaag ggtcataatt agctgcgaga aacgggccat
     3241 gataatggta taggaggcga acaggaaata atctcccagc acgaatccca cgagtcccga
     3301 caaagacatc cagaaccagg tggctttgtc ggcccccacc ggtatgaaac tgccgtttat
     3361 cagcaacatg gtgaatccca gcattaagaa agcacagaca agacggatca ggttcaggtt
     3421 gatggatccg atcctctttc ctgcatattc aaaaaaaagg gcactgagtg tccacaggac
     3481 agcaaccccg atagcaatag tttctccttt catgcgagca caaaagtaga aaaagagttg
     3541 caattatttg actttccatt caatgatccc tccggatgct ccttcctgta attttacgga
     3601 aagctttaag gcacgggtaa tcaagggatt gaactcaagt ttattataac ggtctttagc
     3661 aacagtatat tcggtcaggg cttctaccgg aaaccacctg ttgtcttttt catagtataa
     3721 tttccagctt tcaggtacac ggtaattgcc gtcatagtgg tcaaaatcca gccaataaac
     3781 ttccacacta ccaaccttct caggtttttc gaacacataa cctacagatt cttcacttcc
     3841 atagcgcaac caccagtaga aatacggctt ggaacggtct gccgagttga tgggatccca
     3901 ctggtcgtta aatccgaact cccggactga tagttcttca agagctcctg tccgtgcgga
     3961 aagagcatcc tggtcgct
//
LOCUS       DAOSOF010000158         3942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_23077_length_3942_cov_4.395421, whole genome shotgun sequence.
ACCESSION   DAOSOF010000158 DAOSOF010000000
VERSION     DAOSOF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23077_length_3942_cov_4.395421"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..691
                     /gene="nth"
                     /locus_tag="PLM68_07935"
     CDS             50..691
                     /gene="nth"
                     /locus_tag="PLM68_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT55321.1"
                     /translation="MKKKQEVQHIISWFLTHMEDPGSELQYKDPYSLLVAVILSAQCT
                     DQRVNMTTPALLERYPTVKDMAAARAEDIFPYIRSITYPNNKAQHLAGMARSIMERFD
                     GKIPSDPGELKKLPGVGRKTANVVASVAFDKEVIAVDTHVYRISRRLGLSDGTTSLAV
                     ENDLNHHFPLELRARAHHWLILHGRYVCTAKKPKCSSCGLSPWCDTYKKQNPQ"
     gene            688..1620
                     /locus_tag="PLM68_07940"
     CDS             688..1620
                     /locus_tag="PLM68_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOT55322.1"
                     /translation="MKTIPGTGREWTEAINDFLMYLRLERSLSENTRKAYLQDIKRFL
                     EYMQRSTPPQELFPQPSGITTKHIETFLTHVTDSGSSPVTQARILSGLNAFFRFLTME
                     KRIATNPCSSIDTPKTGRRLPVVLSVREIEMLLDSFDLSIPEGHRNKAIIEVLYGCGL
                     RVSELTGLKFTQMFPQQGFIRVIGKGDKQRLVPIGSHALRALELYYPWRNSLKIRPMH
                     EDFVFLNRYGRPLTRNMVFLIIRQQAEKAGLKKEISPHTFRHSFATHLIENGADLRVV
                     QEMLGHSSILTTEIYTHLDTAYWQHTVLSHHPRP"
     gene            1675..>3942
                     /locus_tag="PLM68_07945"
     CDS             1675..>3942
                     /locus_tag="PLM68_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT55323.1"
                     /translation="MTYRLRTVILTVILLTSGTFYGQAPQHSGDDLFARAVELFRQEQ
                     YWSAYELFNRPETDDMPGKESLVASYRLLCEIAMEDRAIADRVSWWEQKHPSAPLKNL
                     VYFKLGNLYASGNLFKEAITCYDKVKVRRLPEKERASYTFRKGYATMQRGDTEQAVAL
                     FLEAENYPVGESYKWASRYYRGYIHYAEGRFEEAVPLLEPLMEVPQYASLSTAYLLQS
                     LLYLKQYGQVIKKGVPIYQQSDKALRTTLAKTLSEAYFALDRTVEARSFFDDYLKDIS
                     NLTLTDRYFSGILYFKLGDYVQAAEQLSIVGERQDSLGQNALYHLGESYIRLRNKVDA
                     LVSFKRASVMPYDPDIREDAFYNYAKLSFDLNGDIQVLSSYRKEYPDSPKLDEIQTYI
                     AANFFMNQDYASAVEALQALKNPTAENSRDLKKAAFLRGIQLYQTGSFRDAEKYFSLA
                     QEPYWIAECLYRDNKFNNAINIWRQFIKESGSFSNPEKYRTSHYNIGYAYFKMRDYPN
                     AADWFNRYLRLGVSNRSLVADTYLRLGDCAFAQYKHQEAAGEYQRALDYNTAAPDYAL
                     FQIAMAQGVLNLEAEKIITLDRLMQEYPTSPFFSSALFEQGRTYVQTNQYEKAEERFN
                     TILSFDSHRAIHAKALVELGLIRINMQNPDAALDYYKEVLRRYPDSPDAANALAGIEN
                     VYMSRNDSEGFFNYLESLGIDSGKSPDEKEIMAFTSAEQLFLNNSPAAAVTALNKFIG
                     KYPKSVKIPAAYFYLG"
BASE COUNT         1098 a          977 c          921 g          946 t
ORIGIN      
        1 gccaggttgc ggaccgccgt gaaatagact tgtcaggatt tatggaatca tgaaaaaaaa
       61 gcaggaagta caacatatca tttcatggtt cctgacccat atggaggatc ccggatcgga
      121 actgcaatac aaggatcctt acagcctttt ggtagctgtg atcctctcgg cgcaatgcac
      181 ggaccaacgt gtgaacatga ccactcctgc cctgctggag cgctatccta ccgtaaaaga
      241 tatggcagcc gcaagagcgg aagatatctt tccttatatc cgctccatta cctatcccaa
      301 caacaaagca cagcacctgg ccggaatggc gcgcagtatc atggagcgct ttgacggcaa
      361 gattccttcc gatcccgggg agcttaagaa actgccgggt gtcggcagaa aaaccgccaa
      421 cgtagtagct tctgttgcct ttgataagga agtgattgcc gtggatacgc atgtataccg
      481 gatttcacgc aggttgggat tgtcagacgg aactacctca ttggctgtgg aaaacgatct
      541 gaaccatcat tttccactgg aactcagggc aagggcccac cattggctga tcttgcacgg
      601 caggtatgtt tgcactgcaa aaaagcccaa atgttcttca tgtggtcttt ctccctggtg
      661 tgatacctac aaaaaacaaa atccacaatg aaaacgatac ccggaacagg gcgggaatgg
      721 accgaggcaa tcaatgattt tttgatgtat ctgcgactgg agcgatcctt gtctgaaaac
      781 acacgaaagg cgtaccttca ggatataaag cgttttctgg aatatatgca gagaagtacc
      841 ccacctcagg aactatttcc tcaaccttca ggtatcacca caaaacatat tgaaaccttc
      901 cttactcacg tgaccgattc aggatcatct cccgtaaccc aggccaggat cctttcgggt
      961 ttgaatgctt ttttccgctt cctgaccatg gaaaaacgca tagccaccaa tccatgctca
     1021 tcaattgaca cgccaaaaac aggcagacgc cttccggttg tactgtctgt ccgggaaatt
     1081 gaaatgttgc ttgactcttt tgacctgagc attccggaag gacaccggaa caaagccata
     1141 atcgaggttc tctacgggtg cggattacgg gtaagtgaac tgacggggct gaaattcacg
     1201 cagatgtttc cacaacaggg tttcatacgt gtcatcggca agggtgacaa acaacgtttg
     1261 gtgcccatag gttctcatgc actccgtgcc ctggaattgt actacccctg gaggaattca
     1321 ctgaaaatcc gtccaatgca tgaagatttt gtgtttctca accgctatgg cagaccgctt
     1381 acccgtaaca tggtgtttct tataatacgt caacaggcgg agaaagccgg actgaaaaag
     1441 gagatatccc cccacacctt ccgtcattct tttgccaccc atcttattga aaacggggca
     1501 gatcttcgtg tggttcagga aatgctcgga cacagcagta tacttaccac agaaatctat
     1561 acgcacctgg atacggctta ttggcagcat actgtcctaa gccaccatcc aaggccataa
     1621 ttatggtatg attcttgaaa aacagtatat ttgctcaata atcacgtatt acacatgaca
     1681 tacaggttgc gtactgttat tctcactgtt atactcctga cctcaggcac cttctacggc
     1741 caggcaccgc aacactccgg tgatgacctg tttgccaggg cagttgaact cttcaggcag
     1801 gagcagtact ggagtgccta tgagctgttt aacagaccgg aaacggacga tatgccgggg
     1861 aaggaatctc ttgtagcctc ttacaggctt ttgtgtgaaa tcgcaatgga agaccgtgcc
     1921 attgcagacc gtgtttcctg gtgggagcaa aaacatccct cggctccttt aaaaaacctg
     1981 gtatatttca aattagggaa cctgtatgct tccggtaacc tgttcaaaga agcgatcacc
     2041 tgttacgata aagtcaaggt ccgccggctt ccggaaaaag aacgcgcttc atacactttc
     2101 aggaaaggat atgccaccat gcagcgcggt gatacggaac aagccgtagc gctgttcctg
     2161 gaagcagaaa attacccggt gggcgaatcc tacaaatggg cctcaaggta ttaccgggga
     2221 tacatccatt atgctgaagg acgttttgag gaagctgtgc ctttacttga accgcttatg
     2281 gaagtgccgc agtatgcttc cctctccacg gcctacctgc ttcagtcatt attatacctt
     2341 aagcaatacg ggcaggttat caaaaagggt gttcccatat accagcaatc cgataaggca
     2401 ttgcgtacca ccctggccaa gaccttgtca gaagcctatt ttgccctgga caggacagtt
     2461 gaagcacgca gtttctttga cgattacctg aaagatatca gtaacctgac tttgacagac
     2521 aggtattttt ccggaatcct gtatttcaaa ctgggtgatt acgtgcaggc cgctgaacag
     2581 ctctccatag tgggagaaag gcaggacagt ctgggtcaga acgccctgta ccatctggga
     2641 gaatcgtata tccggctgcg gaacaaggta gatgccctgg tttccttcaa aagggccagt
     2701 gtcatgcctt acgaccctga cataagggaa gatgcttttt ataactacgc aaaactctct
     2761 tttgatctga acggagacat acaggtattg tcctcataca ggaaagaata tccggattcc
     2821 ccaaagctgg atgaaatcca gacatatatt gcagctaatt tctttatgaa ccaggattat
     2881 gcctcggctg tggaagccct gcaggcgttg aaaaatccca cggctgaaaa cagcagggac
     2941 ctgaaaaagg cagctttcct gagaggcata cagctatacc agaccggttc tttccgggat
     3001 gctgaaaagt atttttcgct ggcacaggaa ccctactgga tagcagaatg cttataccgt
     3061 gacaataagt tcaacaatgc aatcaacatt tggaggcaat tcatcaaaga gagcggatcg
     3121 ttttccaatc cggaaaaata ccggacaagc cattacaata ttggttatgc ctacttcaaa
     3181 atgcgtgatt accctaacgc tgcagactgg ttcaacaggt atctcagact gggtgtgagc
     3241 aacagaagcc ttgtggcaga tacttacctc aggcttggtg attgtgcttt tgcgcagtac
     3301 aagcaccagg aagcagccgg agaatatcag cgcgcactgg actacaacac ggctgctccc
     3361 gattatgcgt tgtttcagat agccatggcc cagggtgtcc tgaacctgga ggcagagaaa
     3421 atcatcacac tggaccggct gatgcaggaa taccccactt ccccgttttt ttcatcggcc
     3481 ctgttcgaac agggaaggac ttatgtccag acaaaccaat acgaaaaggc agaagaaagg
     3541 ttcaatacca tactttcatt tgactcacac cgggctatcc acgcaaaagc actggtagag
     3601 ctgggactta tcagaatcaa catgcagaac ccggacgcag ccctggatta ttacaaggaa
     3661 gtgctgcgcc gttaccccga ttcccccgat gcagccaacg cactggccgg cattgaaaat
     3721 gtatacatgt cccggaacga ctcggaggga ttcttcaact acttggaaag cctggggatt
     3781 gattcaggaa aaagccctga tgaaaaggaa ataatggcct ttacctcggc cgagcaatta
     3841 ttcctgaata attcaccagc ggcagccgtt accgccctga acaaatttat cggcaaatat
     3901 ccaaaaagtg taaagattcc ggcagcttat ttctatctgg gc
//
LOCUS       DAOSOF010000159         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_23140_length_3933_cov_4.776431, whole genome shotgun sequence.
ACCESSION   DAOSOF010000159 DAOSOF010000000
VERSION     DAOSOF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23140_length_3933_cov_4.776431"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLM68_07950"
     CDS             complement(<1..486)
                     /locus_tag="PLM68_07950"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55324.1"
                     /translation="MLSCLAILNNCSWGTHDAPANLRPYNLDNLNYDVQPAAGSYGFL
                     FYSSADWSAIITCETGEEEIPEMPTVNPSSGRGSSSLQSLNFTIPDNNTEFSRTYTLT
                     ILSADKTLVVVIRQRSALGMLSITPTEVTIPALGGNAAINVESNANWSALVTDDWFTV
                     SP"
     gene            614..1189
                     /locus_tag="PLM68_07955"
     CDS             614..1189
                     /locus_tag="PLM68_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOT55325.1"
                     /translation="MRTTIFYASKYGTTRLIAQEIGELLSTHDQVTLLDVADCKQWPV
                     TDDGFNKAERYVLGVPVYAGKPLRNMQRFCDRYASELEKKPLFLFVCGMERLPEKQQQ
                     EMEAAFPENLRSVAHHVAFLGGALQWKKMNFVERLILAKITGKKGDQDLVSHRNIKKF
                     AEVMVGAQPKSSVTPENSGTDESSGTPGTGS"
     gene            1291..1632
                     /locus_tag="PLM68_07960"
     CDS             1291..1632
                     /locus_tag="PLM68_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOT55326.1"
                     /translation="MKKSLFTLLLLMVLPIVMNANPAHKVTLVYDAETAKLSVTIVHP
                     VMDTEKHFISEITVLVGDKEIEKKELTKQSTKESETYEITLPGLSKGNVITVEAKCNQ
                     FGRKSGKLTIK"
     gene            1826..>3933
                     /locus_tag="PLM68_07965"
     CDS             1826..>3933
                     /locus_tag="PLM68_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT55327.1"
                     /translation="MNLKLPEELNQILSFAREEAMRLGSYTVTGDHLFLAIIRHQENQ
                     AYGILTMLDLLLWQMKNLIESKEMGRTMIPPDQSDKLSLSEHAEKCLKAMYLEASKLG
                     QATPNSLHLLLALLAIDNGIGVYWLRTKGVTDQTLRAYLKGELPLVVKGSQRKRALER
                     KESIKNIDEQMDMALQAAKKQTGSKTGQQKDTSATPMLDMYALDLTEEARAGQLDPVV
                     RRDKEIDRLAQILGRRKKNNPVLIGEPGVGKSAIVEGLANRIARRKGSPTLQGKKILS
                     LDIGSVVAGTKYRGQFEERMKTLLAEIKKNPDIILFIDEIHTLIGAGATGGSGGSLDA
                     ANMLKPALARGEIQCIGASTMDEYREYIEKDGALERRFQKVIVEPTKYDDTLEILRSI
                     RPKYEEHHMVSYTPQALEACVRLSERYITDRCLPDKAIDVMDEAGSHVHIAHLQLPKE
                     LVVMEEEMDSLMMQKREAVDKKDYKTATALREREKKVMEALRQARDTWYSEQKANKKE
                     VNADTVAEVVSMITSVPVSRVSESETQRLLNMSTILKQKLVGQDDAVEAMVRAIHRNR
                     AGLKDPNKPIGTFIFLGPTGVGKTHLAKLLATYMFDSPDSLVRIDMSEYMEKYAVSRL
                     IGAPPGYVGYDEGGQLTERIRRKPYTVVLLDEIEKAHPDIFNLLLQVFDEGRLTDSNG
                     RHVDFRNTIIIMTSNIGSRE"
BASE COUNT         1105 a          947 c         1068 g          813 t
ORIGIN      
        1 gggagatacc gtaaaccaat catccgtgac cagggcactc cagtttgcat tggattccac
       61 attgatcgca gcattccctc ccaatgcggg gattgtgacc tcggtcgggg ttatgctcaa
      121 cataccaagg gctgatcttt gccgtatgac cacgacaagc gttttgtctg cagaaagaat
      181 ggtcagggta taagttctgg aaaattctgt attattgtca ggaattgtga aattcaaaga
      241 ttgcagggat gaggagcctc ggcccgatga aggattgaca gtgggcattt ctggaatttc
      301 ctcctctccg gtttcgcatg tgatgatggc gctccagtct gcagatgaat aaaaaagaaa
      361 accatacgat ccggctgccg gctggacgtc gtaattcagg ttatctaggt tgtatggcct
      421 gaggttggca ggcgcatcgt gcgttcccca tgaacagttg ttcaggatgg ctaagcagga
      481 aagcaaaagg attatattta gaatactcgc tttacgcata gttgaaaaac aattattttt
      541 atatgttaaa tatagtatgt ttttttcaat tgtgttgatt acctttgaaa tacaaacgca
      601 aatctgttaa aaaatgagaa ctacgatttt ttacgcttct aaatatggca ctaccaggtt
      661 gatagcacaa gagatcggag aattactctc tacgcacgat caggtgacac tcctggatgt
      721 tgctgactgc aagcagtggc ctgttacgga tgatgggttc aataaagcgg agcggtatgt
      781 gcttggggtg ccagtttatg cgggcaagcc actcaggaat atgcagcggt tttgtgacag
      841 gtatgcctct gaactggaga agaaaccgct tttcctgttt gtctgcggca tggaaagatt
      901 gcctgagaag caacagcagg agatggaagc tgcctttcct gaaaacctgc gcagtgtggc
      961 ccatcatgtg gcattcctgg gtggcgcgtt gcagtggaag aagatgaatt ttgtggaacg
     1021 cctgatcctc gcaaagatca caggcaagaa aggtgatcag gatcttgttt cgcacaggaa
     1081 tatcaagaaa tttgccgagg tgatggtcgg tgcccagccc aagagcagtg ttacccccga
     1141 aaacagtggc actgatgaaa gcagtggcac ccctgggacc ggcagttgac cggcacgaag
     1201 tgacacggct gattgcagca ggaggccggt aagcattgga acacaggaca accattaaac
     1261 attatgataa acagattaac aaatacacct atgaaaaaat cacttttcac cttattgctg
     1321 ctgatggttt tgccaatagt catgaatgca aatcccgccc ataaggtgac cttggtttac
     1381 gatgcagaaa cggccaagtt atcagtaacc attgtgcacc ctgtcatgga tacggaaaaa
     1441 cacttcatct cagagataac cgtattggtt ggggataagg agattgagaa aaaggagctg
     1501 accaaacaat ccacaaagga atcggaaact tatgagataa cgcttcccgg gctgtctaaa
     1561 ggcaatgtga tcacggtcga agccaaatgc aaccagttcg gccggaaatc aggtaagcta
     1621 acgataaaat aaagacgaca gacagaagca ttaaaggcgc cataagcgcc aaaagcgcca
     1681 aaaacgccag aagcgccata agcgccaaaa acgccataag caccagaagc gcaagaagca
     1741 ttataattgc aggccgcata gcgctgaacc ttgcgataat ataacaggaa actcttattt
     1801 caaagggttt tattaccttt gtggaatgaa tcttaaacta cccgaagaac tgaaccagat
     1861 actctcattc gcacgggaag aggccatgcg tctggggagt tacacggtta cgggagacca
     1921 cctttttctg gccattatcc gccaccagga aaaccaggcc tatggcattc ttaccatgct
     1981 tgatttgctg ctctggcaaa tgaaaaacct catagaaagc aaggagatgg gtcgtaccat
     2041 gattcctccc gatcagtccg acaagctgtc gctttccgag catgcagaga agtgcctgaa
     2101 ggccatgtac ctggaggcaa gcaagctggg gcaggcgacc ccgaattccc tgcacctgct
     2161 gcttgcgttg cttgccatag acaacggaat tggcgtttac tggttgcgca ccaagggagt
     2221 gacggatcag acgctgcgtg cctacctgaa gggtgaattg ccccttgtgg tgaaaggttc
     2281 ccagcgcaag cgtgcactgg agcggaaaga gtccatcaag aacatagatg aacagatgga
     2341 catggctttg caggcagcta aaaaacaaac cggcagcaag accgggcagc aaaaggatac
     2401 ctcggcaaca cccatgctgg acatgtacgc gctggacctg accgaggaag cacgtgccgg
     2461 gcagctggac ccggtggtgc ggcgtgataa ggagattgac cgcctggcac agatcctggg
     2521 tcggcgcaag aagaacaacc cggtgctcat tggcgagccc ggagtgggta aatcggccat
     2581 tgtggaaggc ctggccaacc gcattgcccg gcgcaaaggc tcaccaacgc tgcaaggcaa
     2641 gaaaatcctc tcgctggaca tcggctcggt ggtggccggc accaaatacc gcgggcaatt
     2701 cgaggaacgc atgaaaacgc tccttgccga gataaagaag aatcccgata tcatcctttt
     2761 cattgacgag atccataccc tgataggggc aggcgccacg ggggggagcg gtggctcgct
     2821 ggatgccgcc aacatgctca aaccggccct ggcacgggga gagatccagt gcatcggggc
     2881 cagcaccatg gatgaatacc gggagtacat tgaaaaagac ggtgccctgg agcggcggtt
     2941 ccagaaagtc atcgtggaac ccaccaaata cgacgataca ctggaaatcc tgcgcagcat
     3001 acgtcccaaa tacgaagaac accatatggt gagctatacg ccccaggcac tggaggcctg
     3061 cgtgcgcctt tccgagcgtt acataaccga ccgctgcctg cccgacaagg ccattgatgt
     3121 gatggacgaa gccggatcgc atgtgcacat agcccacctg caattgccca aagaactggt
     3181 agtcatggaa gaggagatgg attcactgat gatgcaaaag cgggaagccg ttgacaagaa
     3241 ggattacaag acggcgacgg ccctgcggga gcgggaaaag aaggtaatgg aagcactgcg
     3301 ccaggcaagg gatacatggt attcggaaca aaaggccaac aaaaaagagg tgaatgccga
     3361 cacagtggcc gaagtggttt ccatgatcac ctcggtaccc gtaagccgtg tctcggaatc
     3421 ggaaacacag cgtttgctca acatgtctac catactcaaa cagaagctcg ttgggcagga
     3481 cgatgccgtg gaagccatgg tacgggccat ccaccgcaac cgcgccggat tgaaggatcc
     3541 caacaagccc atcggcacgt tcattttcct gggacccacc ggggtgggca agacgcacct
     3601 ggcaaaactg ctggccacct atatgtttga ttctccagac agcctggtgc gcattgacat
     3661 gagcgaatac atggaaaaat atgccgtcag caggctcatt ggcgcgcctc cgggatacgt
     3721 gggatacgac gaggggggac agctgacaga gcgcataaga cgcaaaccgt ataccgtggt
     3781 ccttctggac gagatagaga aggcacatcc cgatattttc aacctgctgt tgcaggtctt
     3841 tgatgaggga cgcctgaccg acagcaacgg acgtcatgtg gatttccgca acaccatcat
     3901 catcatgacc tccaacattg gcagccgtga att
//
LOCUS       DAOSOF010000160         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_23241_length_3919_cov_3.739389, whole genome shotgun sequence.
ACCESSION   DAOSOF010000160 DAOSOF010000000
VERSION     DAOSOF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23241_length_3919_cov_3.739389"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PLM68_07970"
     CDS             <1..1004
                     /locus_tag="PLM68_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT55328.1"
                     /translation="MMSRYHQAMSAVFESAVSNTVPEIYRNEIFTSPRVAFIFCTTKG
                     DVDSFRELSDQTLNPRLYPWHVSQQMMNELNLHGKPYVVMNSAMSGMQAQIVANRLLC
                     KGSPYDFAVIIGVELISMFTVFLLSQAGLVSNKPVRPFDVEGNGTNPGESASCAIFVS
                     EVRFQKDKKHLNMPCFIKFKEGAVMTDPCNGGSFDTKGQALLRVIQKVMIRENARNLS
                     FINAHGLGLTVIDNITSIAIEDAGLRRVPVNCFEGSFGHSSGSTSLMNSILGAKSLEE
                     GIILPTLGYRTHGVDGRIQIVTTVKTSNKNSFLNVSTGFGGVNAATLYTKEKNHHAH"
     gene            994..1629
                     /locus_tag="PLM68_07975"
     CDS             994..1629
                     /locus_tag="PLM68_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55329.1"
                     /translation="MLIKSFCRITNQSIVLDRARRPELEKLMMGNHVVEDLDRIFLFL
                     KVDFPSYKNMDDYSKLGFITAELLMRHNGYDPSVSKPDMGLFIYSRSGCLGADYNLVR
                     VMEGLHADDQAYKRSIPNAVCSDIALRHNIQGEMGIFLSDGFIYEQFFRTCFGTLETE
                     PSLNTLLAGYLECYGGYASSLLCLLTRSPEHPPMDYDLREERFCIDLLRIY"
     gene            1759..3036
                     /locus_tag="PLM68_07980"
     CDS             1759..3036
                     /locus_tag="PLM68_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55330.1"
                     /translation="MIEDFPEHLRPYTDEQIPAAMQRIAAHRYFPDVAAMIFPGITPA
                     EAISKVLEVKTVYQFQMEWMYAFNKRVIESTMESFTYDFSPGIRPDVGYLYISNHRDI
                     ILDTSLLQMIFVDQGLPTSMITYGDNLIVNQLADDLARSNKMFKVVRQGNKRELFKNS
                     VLLSEFLRSCIRQGDSCWIAQRNGRTKDGLDKTAPALVKMMGMSGMRGAAATSDGVAA
                     GGGLNELVENYAALNIVPVAISYEYESCDFLKTKELLAAQNAPDGKTYIKDEDEDLIS
                     MITGISQWKGKVHIHVCDPLTREELEACVESAGLGPGEKVVSGGAVRHGGQDFNAFAA
                     NLCTLIDSKIWHNYKCFPSNYIAYDLLHGSSTHKHHYSDQNKAAFLKRLEALPQKVFG
                     SNADEALQEALKAAQHIFLKIYANPVRRSAAHS"
     gene            complement(3139..3690)
                     /locus_tag="PLM68_07985"
     CDS             complement(3139..3690)
                     /locus_tag="PLM68_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOT55331.1"
                     /translation="MRCRPGNFCYSHAEVETMCESIRLCRQIGVRGVVIGALTSKGDP
                     DLPALSRMIHAATGVSATTPGPGPATASTADIAIATNPEPRPLTLVFHRAFDECRDPF
                     GALEQIINLSFSTILTSGQAPNAEAGIPMLCQLVDKAAGRITIMAGKGVTADNVAKII
                     RQTGVPAVHLSERPGIPEILVRI"
     gene            3655..>3919
                     /locus_tag="PLM68_07990"
     CDS             3655..>3919
                     /locus_tag="PLM68_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55332.1"
                     /translation="MRITKIAGTAPYKNVRLLQKFFQRDAGLLRSGLQASWKGDGLLP
                     GGWEGDGLLPGGWKGDGLLPGGWKGDGLLPGGWKGDGVQQVEPG"
BASE COUNT         1001 a          910 c         1079 g          929 t
ORIGIN      
        1 acatgatgtc aaggtaccac caggccatgt ctgccgtttt tgaatctgcc gtatccaata
       61 ccgtacccga gatctacagg aacgaaattt ttacgagtcc ccgtgtagcg tttatttttt
      121 gtaccaccaa gggagatgtc gactcgttcc gcgaactgag tgatcagacc ctaaatccca
      181 ggctctatcc ctggcacgtg tcccagcaaa tgatgaacga attgaatttg cacgggaaac
      241 cctatgtggt tatgaattcc gccatgtccg gcatgcaggc ccagatagta gccaaccggc
      301 tgttatgcaa aggcagtccc tatgattttg ccgtgatcat aggggttgag ctgatcagta
      361 tgttcaccgt ttttctttta agccaggccg gccttgtaag caacaagccg gtacggccgt
      421 ttgatgttga aggcaatggt actaatccgg gcgaatctgc ctcctgtgcc atatttgtaa
      481 gtgaagtgcg gtttcagaaa gacaaaaagc atctgaacat gccgtgcttt attaaattca
      541 aagagggtgc cgtcatgaca gatccatgca acggcgggag ctttgacact aagggtcaag
      601 ctttgctaag ggtcattcag aaagtgatga tccgggaaaa tgccaggaac ctttctttta
      661 tcaatgcgca cgggttgggt ctgacagtta tcgacaacat cacatccatt gccattgagg
      721 atgccggctt acgcagggtg cctgtcaatt gttttgaagg cagctttgga catagttccg
      781 ggtctacgag cctgatgaac agcatactag gagctaaatc gctggaagag gggatcatat
      841 tgcccaccct gggttacaga acccacgggg ttgatggccg gatccagatc gttactactg
      901 tcaaaacctc gaacaagaat agttttctca atgtatccac cggttttgga ggagttaatg
      961 ctgcaaccct ttatacaaaa gaaaagaatc atcatgctca ttaaatcgtt ttgcaggata
     1021 accaaccaat ccattgttct ggaccgggca aggcgcccag aactggaaaa actcatgatg
     1081 ggcaaccatg tggttgaaga ccttgaccgc atttttttat ttctgaaagt ggattttccc
     1141 agctataaaa acatggatga ttatagcaag cttggattta taaccgcgga attgcttatg
     1201 agacacaacg gttacgaccc ttctgtgagc aaacccgata tgggactatt catatactcc
     1261 cgctctggtt gcctgggggc cgattacaac cttgtacgcg tgatggaagg actacacgca
     1321 gacgaccagg catataaacg ctccataccc aatgcagtat gcagtgatat tgcattaagg
     1381 cacaatatac agggcgagat gggaatattt ctttcagacg gattcatata cgagcagttt
     1441 ttcagaacat gttttggcac cctggagacc gagccctcgc tgaatacatt attggcagga
     1501 tatttggagt gttacggggg gtacgcatca tcccttctgt gcctgctcac gcgttcaccc
     1561 gaacatccac ccatggatta tgacctgagg gaagagcggt tttgcataga tctgctgcgg
     1621 atatattgat ggatgcaggc tgcgtacgcg gtggctggtg caggaagggt gattgtgaag
     1681 gagggcgggt tgccccggtg gtttgccgcg gtggagaaag gtggttgctg cgtgcagttg
     1741 ttttaaattt tttaagacgt gatagaagat ttcccggaac atttaagacc ttataccgat
     1801 gaacagatac cggcagccat gcagcgtatt gctgcgcacc ggtactttcc ggatgtggca
     1861 gcaatgatct ttcccggcat cacaccggca gaagccatca gtaaagtgct ggaagtgaag
     1921 accgtttatc agttccagat ggagtggatg tatgcattca acaagcgtgt gatcgagtcc
     1981 actatggaaa gtttcacata cgatttcagc cccggtatca gaccggatgt cggctatctt
     2041 tacatatcta accacaggga tatcatcctg gacacttctt tactgcagat gatatttgtt
     2101 gaccagggac tgcccaccag catgataacc tacggggaca accttattgt gaaccaactg
     2161 gcagatgatc ttgctcgctc caacaagatg ttcaaggtgg tcaggcaggg caacaaacgg
     2221 gagttgttca agaactcggt cctgttgagc gaatttctgc gcagctgcat acgtcagggc
     2281 gactcctgct ggattgccca acgcaacggc cggacaaaag acgggctgga caagacagcc
     2341 ccggcactcg tcaaaatgat gggaatgagc gggatgcgag gtgctgctgc aacgagtgat
     2401 ggggttgctg ccggaggcgg gctgaatgaa ctggttgaaa actatgccgc cctgaacata
     2461 gtgcccgtag ctatttccta cgaatacgaa tcatgcgatt tccttaagac aaaggaactg
     2521 ctggctgctc aaaatgcacc tgatggcaaa acctacataa aggacgaaga cgaagacctg
     2581 atcagtatga taaccgggat ctcgcagtgg aagggcaaag tacacatcca tgtatgtgat
     2641 ccgctaacac gcgaagaatt ggaagcatgt gttgaaagtg ccgggcttgg tcccggtgaa
     2701 aaggtagtaa gtggcggtgc cgtgcggcat ggcggacagg acttcaatgc attcgccgct
     2761 aacctctgca ccctgattga cagcaagata tggcacaatt acaaatgctt cccatccaat
     2821 tatattgcct acgatttact gcacggcagt agcactcata aacaccacta ttcagaccag
     2881 aacaaagctg ccttcctcaa gcgcctggaa gccctgccgc aaaaggtttt tggtagtaat
     2941 gccgatgaag cgttacagga agctttaaag gcggcacaac acattttcct gaaaatttat
     3001 gccaatccgg tccggaggag tgccgcgcac tcttgatgta ttgatctgta tgacagatga
     3061 ttgacaaaat agctctccag gagagctctt ttgtcatatt attgataatc agtaagatga
     3121 atgaagagtg ctcggcagtc agatgcgcac aagaatttca gggatacccg gccgctccga
     3181 caggtgaacc gccggaactc cggtctgtcg gattatttta gcaacattgt cagcagtaac
     3241 gcccttgccg gccataattg tgatgcgtcc agctgcctta tccaccagtt ggcagagcat
     3301 ggggatcccg gcctcggcat tcggcgcctg acccgaggtc aaaatggtgc tgaagctcag
     3361 gtttatgatc tgttccagcg ctccgaatgg atccctgcat tcgtcaaatg cccggtgaaa
     3421 caccaacgtc aaaggccgag gctcggggtt agtggcaata gcaatgtcag ctgtagaagc
     3481 agtagcgggg ccaggcccgg gggtagtggc actaacaccg gtggcagcat ggatcatgcg
     3541 gctaagggca gggaggtcgg ggtcaccttt ggaggtgagc gctcctatga ccacgccccg
     3601 cacaccgatc tggcggcata ggcgaattga ttcgcacatg gtttctacct cggcgtgaga
     3661 ataacaaaaa ttgccgggac ggcaccgtat aagaacgtgc gtctgctcca gaaattcttt
     3721 cagagagatg ccgggttgct gcgcagcggg ttgcaggcta gttggaaagg tgacgggttg
     3781 ctgccgggcg gatgggaagg tgacgggttg ctgccgggcg gttggaaagg tgacgggttg
     3841 ctgccgggcg ggtggaaagg tgacgggttg ctgccgggcg ggtggaaagg tgacggggtg
     3901 cagcaggttg agccaggtg
//
LOCUS       DAOSOF010000161         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_24919_length_3708_cov_4.522584, whole genome shotgun sequence.
ACCESSION   DAOSOF010000161 DAOSOF010000000
VERSION     DAOSOF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_3708_cov_4.522584"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLM68_07995"
     CDS             complement(<1..383)
                     /locus_tag="PLM68_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOT55333.1"
                     /translation="MIHKLSDIHPKAILGNNVEVGPFTTIAQHVEIGQGTVIGPNVTI
                     MDYVRIGANCKVFPGAVIGAIPQDLKFAGEVSTVEIGDNVTIREYATINRGTAASKIG
                     VTRVDNNCLVMSYVHIAHDCHVKNNV"
     gene            complement(373..1287)
                     /locus_tag="PLM68_08000"
     CDS             complement(373..1287)
                     /locus_tag="PLM68_08000"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOT55334.1"
                     /translation="MKQQTICGEISFSGVGLHSGTTVEMSLCPAPEGYGIRFQRTDIP
                     GDPVVEALAVYVAKTERRTVLMRDQVGISTPEHLLAALYGMGVDNVLVKLNGPEVPIL
                     DGSAYPFAAGIKATGVHEQKAGREYIDLIHPVHFSDKNSGTTIDVVPSSSFSARVILD
                     FDSGVLGKQEFVFNQETDFLSAVACSRTFVFLHEVLPLFEKGLIRGGDLDNALVIEDK
                     ELSEQDLFQLRKMFGKQGLIMHKGYLNEAELRFPDECARHKMVDLLGDLFLAGKRFNA
                     SVTAYKPGHASNTAVALMISDQMNGYDT"
     gene            complement(1284..2273)
                     /gene="lpxD"
                     /locus_tag="PLM68_08005"
     CDS             complement(1284..2273)
                     /gene="lpxD"
                     /locus_tag="PLM68_08005"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOT55335.1"
                     /translation="MELTAGIIAKHLKGEVAGDPDVVVTAMARIEQGKKGTACFLANP
                     KYEKYLYQTKASIVLINRDFTPTGDVSATLVKVDNAYAAAAELLGLFSSEKKKSGWEF
                     PHFISRRTSIGRKVYIGAHSYIGKRVRIGNGAQIYPQVYLGENVRIGAGTILYPGVKV
                     YDDCIIGERCIIHANAVIGSDGFGFSPMADGTYRKIPQTGNVIIEDDVEIGANTVIDR
                     ATMGSTIIRRGVKLDNLIQIAHNVEVGENTVMAAQSGIAGSTRLGANCMIAGQAGIVG
                     HLTIADGTQVGAQAGIIGSVKEKGSALLGMPAIDHSQFMRAYALFRKSGKKDK"
     gene            2449..3693
                     /gene="nusA"
                     /locus_tag="PLM68_08010"
     CDS             2449..3693
                     /gene="nusA"
                     /locus_tag="PLM68_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT55336.1"
                     /translation="MEGLNLISTFAEFKELKNIDRPTMMSVLEDVFRSQLVKMYGNAD
                     NFDIIINIDKGDFEIWRSREVVETVEDPNTQISLEEVSRIDNSYEVGESYTDEVKLSE
                     FGRRGILTLRQNLAGRIMDIEKANMYAKYKERVGDIIVGEVYQVWKKEVLVLDEEGNE
                     LLLPKSEQIPSDYFRKGDTVRAVVSNVEMRNNSPVITLSRTSPKFLERLFEQDVPEIY
                     DGLITIKKIVRIPGERAKVAVESYDDRIDPVGACVGVKGARIHGIVRELRNENIDIIN
                     WTSNPQLLIQRALSPAKISSIKINEEAKRVGVYLKPNEVSLAIGKGGSNIKLAGMLIG
                     YEIDVFREMDEDEEDVMLDEFNDEIDQWIIDSLKQIGCDTAKSVLAIPIPELVKRADL
                     EEETVTEVIRILRAEFENDTKE"
BASE COUNT          992 a          897 c          847 g          972 t
ORIGIN      
        1 acattatttt ttacatgaca atcatgagct atatggacat aactcattac caggcaatta
       61 ttatctaccc tggtcacgcc tatcttgctt gcggccgttc cacggttaat ggtggcatat
      121 tcgcggattg tcacattgtc tccgatttcc acggtcgaca cctctcctgc aaatttcagg
      181 tcctggggta tcgccccgat aaccgctccc ggaaaaacct tgcaattggc ccctatgcgc
      241 acgtagtcca ttatggtcac attgggtccg ataacagtcc cctgaccaat ttccacatgt
      301 tgtgcaatgg tagtgaaagg ccccacttcc acattattcc ccaggatggc tttggggtga
      361 atatcactaa gtttatgtat cataaccatt catttgatca ctgatcatga gtgcaacagc
      421 agtattggat gcatgtcccg gtttgtaggc agtaacagag gcattgaaac gttttcctgc
      481 caggaaaagg tctcccagca ggtcaaccat tttatgcctg gcacattcat cgggaaaacg
      541 caattccgcc tcattaaggt agcccttatg cattatgagg ccctgtttgc cgaacatttt
      601 ccgaagctgg aacaggtctt gttcactcaa ctccttatct tctattacca gcgcattgtc
      661 caggtcgcct cccctgatca atcctttttc aaacagaggc agcacttcgt gcaggaatac
      721 aaaagtccgg ctgcaagcta ccgcagacag gaagtctgtt tcctggttaa acacaaattc
      781 ctgtttgcca agcactcccg aatcgaaatc aaggatcacc cgggcagaaa aagaagatga
      841 cggaactaca tctatagtgg tcccggaatt cttatcagag aaatgtaccg gatggatcag
      901 gtcaatatat tctcttccgg ctttttgttc atggactccg gtagccttta tgcctgctgc
      961 aaaagggtaa gcgctcccgt ccaggatggg gacctcgggt ccattcagct ttaccagtac
     1021 gttgtcaact cccatgccat ataaggcagc caggaggtgt tcaggggtgc tgatcccgac
     1081 ctgatcccgc attaagacag tccttctttc agttttggca acatatacgg ctagggcttc
     1141 cacaaccgga tctccgggaa tatccgttct ttgaaaacgg atgccgtaac cctccggggc
     1201 cgggcacagt gacatctcaa ccgttgttcc tgagtgaaga cctactcctg aaaatgagat
     1261 ttccccgcaa atcgtctgtt gcttcatttg tcttttttcc cggatttcct gaaaagggca
     1321 tatgccctca taaactggct gtgatcaatg gcgggcattc ccagcagggc actgcctttc
     1381 tctttgacag aaccgatgat accggcctgc gctccaacct gcgtgccgtc agcgatagtc
     1441 aggtgcccaa ctatccctgc ctgccctgct atcatgcaat ttgcccctaa gcgggtagaa
     1501 ccggctatgc cactctgggc tgccatcacg gtgttctcac cgacctcaac attgtgggct
     1561 atctgtatca ggttatccag ctttacccca cgcctgatta tggtagatcc catggtggca
     1621 cggtcaatga ccgtattggc tccaatttcg acatcatcct ctatgatcac gttgcctgtc
     1681 tggggaattt tcctgtaagt gccgtcagcc atgggcgaaa acccaaagcc gtcagatcct
     1741 atgaccgcat tcgcatgaat gatgcagcgt tcccctatga tacagtcgtc gtataccttg
     1801 acccccgggt agaggatggt tcctgcccct atcctgacat tttcccctaa ataaacctgg
     1861 ggatagatct gggcgccgtt ccctatacgc acacgcttcc cgatataact gtgcgctccg
     1921 atataaacct tccttcctat ggaagttctc cgggaaataa agtggggaaa ttcccagccc
     1981 gatttctttt tttcagaaga gaacagcccc agcaattctg cagctgcagc ataggcattg
     2041 tcaactttga caagtgtggc ggatacatcc ccggttggag taaaatcccg gttgatgagc
     2101 actatggatg ccttggtctg ataaaggtat ttttcgtatt tcggattggc taaaaaacag
     2161 gctgtgcctt tttttccttg ctctatgcgc gccatggccg ttacgaccac atccggatcc
     2221 cctgcaacct cgccttttaa atgcttggca ataataccag ctgtcagttc cataatatct
     2281 tatatgttaa taaacttcaa caaatttcgt tccattaatc aaataatgca aataagaaaa
     2341 taaatttgcc ggtatccggt aaaaatgctt acatttgcag ccgattagga acacgtgcaa
     2401 gaggaccttg gtcctctttt ttattaaaat aaaaaaagtt tacccaaaat ggaaggttta
     2461 aatctgatca gtacatttgc cgaattcaag gaattgaaaa acatagaccg ccctaccatg
     2521 atgagcgttc tggaagatgt tttccgttct cagttagtca aaatgtatgg aaatgcagat
     2581 aatttcgaca tcattatcaa tatcgacaag ggagattttg agatatggag aagcagggag
     2641 gtggttgaga ctgttgaaga tcccaacacc cagatctctc ttgaagaagt atccagaatt
     2701 gataattcct atgaagtagg cgaatcctac acagacgaag tgaaactctc tgaattcggc
     2761 cgcaggggaa ttctcacgct caggcaaaat cttgccggac gcatcatgga tattgaaaag
     2821 gccaatatgt atgccaaata caaggagcgt gtgggagata tcattgtcgg ggaagtctac
     2881 caggtctgga aaaaggaagt gctcgtattg gatgaagagg gcaatgaact gctgttgcca
     2941 aaatcggagc agatccccag tgattatttc cggaagggag atactgtaag ggctgtcgtt
     3001 tccaatgtgg aaatgaggaa caattcacct gtcatcaccc tttccagaac atccccgaaa
     3061 ttcctggaac gtctttttga acaggatgtt cctgaaatat acgacggact gatcactatc
     3121 aagaaaattg ttcgcattcc cggtgaacgc gccaaggtgg ccgttgaatc ttacgatgac
     3181 cgtattgatc ccgtaggcgc ctgtgttggt gtaaagggcg ccaggatcca cgggattgtc
     3241 cgtgaattga ggaacgaaaa cattgatatc atcaattgga caagcaatcc ccagctgctt
     3301 attcaaaggg ctttgagtcc ggccaagatc tcgtcaatca agatcaatga agaagcaaaa
     3361 cgggtcgggg tatacctcaa acctaacgaa gtgtccctgg ccattggcaa gggaggatcc
     3421 aacatcaagc tggccggaat gctcataggt tatgaaattg atgtgttccg tgaaatggac
     3481 gaagatgaag aagatgtcat gctggatgaa ttcaacgatg aaatagacca gtggatcata
     3541 gactcactca agcagattgg ttgtgacaca gcaaaaagtg ttctggctat tcctatcccg
     3601 gagctggtca aacgtgcaga tctggaagag gaaacagtta cggaagttat ccgtatcctg
     3661 agggcggaat ttgaaaacga tacaaaagaa taaggagatc atatgtcg
//
LOCUS       DAOSOF010000162         3677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_25214_length_3677_cov_5.110712, whole genome shotgun sequence.
ACCESSION   DAOSOF010000162 DAOSOF010000000
VERSION     DAOSOF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25214_length_3677_cov_5.110712"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1471)
                     /locus_tag="PLM68_08015"
     CDS             complement(5..1471)
                     /locus_tag="PLM68_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOT55337.1"
                     /translation="MKTKLYRLTVIAVFGVLLMGCTKDFKKINTNPNAAADVPIANVF
                     AYCIRYTCSTHFDPWADMNEPSTYGGHIAKKQYIDEARYVFRPAVVQNNWTYLYRILN
                     NLRDIRTRAEADNLTNMVNVSKIWEAVVMQLGTDRWRDLPYSEAIQLESGVVSPKYDT
                     QEEIYPALMALLKEAADGLNAGGTDDISAGDIFYNGDTDAWLRLANSIRLRMALRIAN
                     VNPTMAKQVVEEITGNPGKYPLIKENSQNAFFYWPGSSPYFEPWADDFRTRDDHCISD
                     ILVNVMLDLDDPRLPVYAQPNGYGEYAGCEIGGPDAITTGHAMKYSLIGTRFREDMGG
                     FSPIYRAAETYFHLAEAAVRGWNVGVSAKNAYDAGITTSLEENGVESEAAGYLAGKAA
                     FDGTLNQIYLQEWICLFKQGMETWSLYRKTGIPTTHHIARSMEECGYTGHNTPPLRYP
                     YPLIESSLNGDNSAGPMADVVDDFWGKQMWFDTRTGLK"
     gene            complement(1490..>3677)
                     /locus_tag="PLM68_08020"
     CDS             complement(1490..>3677)
                     /locus_tag="PLM68_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT55338.1"
                     /translation="ATPWVSHPDNIKSFFNTGYSQSHNISVITRTKGATTRASLGFRD
                     QKGTLPNTDQTRVSAQVNSNVEINKYISFDLAMNYTMTQSENLPQGAYNAGNPLQSLL
                     QWFGRQVNMESLKNLYDKGNDPYTGKPYSWCPDYHQNPYYTMYYNTNSFERNRFFGKT
                     SLWFKPTSWLKFEGRLGYDYYDTYTKQVVLYHTDYPDGGFWSYNQKNSEINADFLAYF
                     DKTFGDNLLSVNAVLGANYRDLDYKTSSLSAEALIVPGLYTISNVAGSPGTNMGGSHI
                     RSNSVYASLSLGFKGMLYIDASARNDWSSTIADPFFYPSVSGSWIITETFPVLKGTVL
                     EFLKLRGGWAKIGAATGAYQTDRYYSSVGYNIHGVGQFYNPTTYPPAGLRPESVETSE
                     IGLEARFFDNRLGFDVAMYNKNTTDQILNVEVSRATGYNAMIINAGEINNKGIEVQLT
                     ANPVRTSDFNWNITLNWSMDRSKILYLYENEESKQLIDVYTIGSSWSVYTQARKGEPW
                     GAIYGTGSVTDEDGNIVVGANGRAKRQSKVLGNVNPDWIAGLQMDFTWKNLSLGVMFD
                     FRKGGDFFSVSQMFGAYTGIYDYTAANGVRENGVVFGKDILTDRKFVKEDGSVNDIVV
                     APTSAFSDFYSNRSYSVIDGSYLKLKDFYITYTIPASRISKNNLVKAFNVSLVGNNVA
                     ILWLHKSNITRVDPESSMNSANSGVGMESNAYLPTRSIGLKVGITF"
BASE COUNT          899 a          879 c          891 g         1008 t
ORIGIN      
        1 ggttttattt taaaccggta cgggtgtcaa accacatttg ctttccccag aaatcatcaa
       61 caacatcggc catgggtccg gctgaattgt ctccgttgag cgaggactca atgaggggat
      121 agggataacg cagaggaggt gtgttatgtc ctgtatagcc gcattcctcc atggatctgg
      181 caatatggtg ggtggtggga ataccggtct tcctgtacag ggaccaggtt tccatcccct
      241 gcttgaagag acagatccat tcctgcaggt agatctggtt gagcgttccg tcaaaggcag
      301 cttttccggc caggtacccg gcagcctctg attcaacacc gttctcttcc agggaagtgg
      361 ttattccggc gtcataggca ttttttgccg agacaccgac attccagccg cggacagcgg
      421 cttcggccag atggaaatag gtttccgctg cgcggtatat cggcgaaaag ccgcccatat
      481 cttcacggaa acgtgtacca atcagagagt atttcatcgc atgcccggta gttatggcat
      541 cgggtcctcc gatctcacac cctgcatatt caccataacc gtttggctgg gcataaacag
      601 gaagccgggg atcatccaga tcaagcatta catttaccag gatatccgag atgcaatggt
      661 cgtcacgagt ccggaaatca tctgcccagg gctcaaaata aggggaagat cccggccagt
      721 agaagaacgc attctggctg ttttccttaa taagaggata ttttccagga ttacctgtaa
      781 tctcttccac tacctgcttt gccattgtgg gattgacatt ggctatgcga agcgccatac
      841 gcaggcggat ggaattggcc agacggagcc aggcatctgt atctccgttg tagaaaatat
      901 ctccggcact gatatcatcg gtgccaccgg cgttcagtcc gtctgcagct tctttaagca
      961 atgccatcag ggcaggatag atctcttcct gggtgtcgta cttgggagag actacgccac
     1021 tctccagctg gattgcctct gaatagggca ggtcacgcca cctgtcggtc ccaagctgca
     1081 ttacgacggc ttcccatatc ttggatacat tgaccatatt ggtgagattg tccgcctcgg
     1141 ctctggtcct gatatcccta aggttattca ggatcctgta aagatacgtc cagttatttt
     1201 gtactacagc gggacggaat acataacggg cttcgtcaat gtattgtttt ttggcaatat
     1261 gaccgccata ggtgctcggc tcattcatgt ctgcccaggg gtcaaaatgc gtgctgcagg
     1321 tataccggat acaataagca aacacattgg ctatgggcac atccgcagcg gcgttggggt
     1381 ttgtgttgat tttcttgaaa tctttagtac accccatcaa cagaacaccg aaaacggcga
     1441 taacagttaa tctatacaat tttgttttca tttcttcaat tttttggggt taaaaagtga
     1501 tacctacttt aagaccgata ctgcgggtag gcagataagc attggattcc atacccacac
     1561 cgctgtttgc ggaattcatg ctcgattcag gatcaaccct ggtaatgttg gatttatgca
     1621 gccacaggat ggctacatta ttaccaacca aagatacatt gaacgcttta acgaggttgt
     1681 tcttggagat tcttgaagca ggaatggtgt aggtgatata aaagtccttc aatttcaggt
     1741 aagaaccgtc aatgacagag taagaacggt tgctgtaaaa atctgaaaat gcagacgtcg
     1801 gggcaactac tatgtcgttg accgaaccgt cttccttgac aaattttctg tcggtgagga
     1861 tgtctttacc aaagaccaca ccgttttcac gcaccccgtt ggctgctgtg taatcataaa
     1921 tccctgtata agccccgaac atctgtgaaa ctgagaaaaa atctcctcct ttacggaagt
     1981 caaacattac tcccaatgaa agatttttcc aggtgaaatc catttgcaga ccggcaatcc
     2041 agtccgggtt gacgttgccc agcaccttgc tttggcgttt tgcacggccg ttggctccta
     2101 caacaatatt cccgtcttca tcggttacag atcctgtccc gtaaatggca ccccagggtt
     2161 cacccttgcg tgcctgtgta tatacagacc atgaggaacc tatggtgtaa acatctatca
     2221 gctgtttgga ttcttcattc tcatacaggt aaaggatctt gcttctgtcc attgaccagt
     2281 tcagggtaat gttccagtta aaatcactgg ttctcaccgg attagctgta agttgaacct
     2341 caataccctt gttgttgatc tccccggcat tgattatcat ggcattgtac cctgtggcac
     2401 gcgatacctc tacgttgagg atctgatctg ttgtattttt gttatacata gccacatcaa
     2461 agcccagccg gttatcaaag aacctggctt ccagacctat ttctgatgtc tcaacagatt
     2521 cgggacgaag ccctgcgggc ggatacgtgg tggggttgta gaactgtccc accccgtgga
     2581 tattgtatcc cactgaagag taatacctgt ctgtctgata agccccggtg gctgctccga
     2641 tcttagccca gcctccgcgc aatttcagga actcaagtac cgttccttta agaacgggga
     2701 atgtttcggt tatgatccag ctgcccgata cggagggata aaagaaagga tctgcaatgg
     2761 tggaactcca gtcgttgcgg gcagaggcat ctatatagag cattcccttg aaacccagcg
     2821 acaggctggc atatacggaa ttggaacgga tatgggatcc tcccatgttt gtaccgggac
     2881 ttcctgccac attggatatg gtatacaaac ccggaacgat caaagcttct gctgacagag
     2941 aggatgtctt atagtccagg tccctgtaat tggcccccaa aacagcattc acactcaaca
     3001 ggttgtctcc gaacgttttg tcaaaatagg ccagaaaatc tgcattgatc tcagaatttt
     3061 tctggttata ggaccaaaaa ccgccgtcag gatagtcggt gtggtacaga accacctgct
     3121 ttgtatacgt gtcgtaatag tcatagccca ggcggccttc aaatttgagc cagcttgtag
     3181 gcttgaacca taacgaggtt tttccgaaga acctgttgcg ttcaaagctg ttggtattgt
     3241 agtacatggt ataataagga ttctggtggt agtcgggaca ccagctgtat ggcttgcccg
     3301 tgtacggatc attgccttta tcatacaaat tcttaaggga ttccatgttt acctggcgcc
     3361 cgaaccactg cagcaacgat tgcaggggat tcccggcatt gtatgctccc tgcgggaggt
     3421 tttcactctg ggtcattgtg taattcatgg ccagatcgaa tgagatgtat ttgtttattt
     3481 ccacattcga attaacctgt gcgctcacac gtgtctggtc tgtattggga agggttcctt
     3541 tctggtcccg gaaacccagt gatgcacgtg tggtggcccc tttggtcctg gttattactg
     3601 aaatgttatg actttgcgag tagccggtat taaagaagct tttaatgtta tctggatgcg
     3661 aaacccaggg ggttgcc
//
LOCUS       DAOSOF010000163         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_25716_length_3623_cov_4.915639, whole genome shotgun sequence.
ACCESSION   DAOSOF010000163 DAOSOF010000000
VERSION     DAOSOF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25716_length_3623_cov_4.915639"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="PLM68_08025"
     CDS             <1..1752
                     /locus_tag="PLM68_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT55339.1"
                     /translation="AFKMNQNRFPWNECSVPSDRFYDCFDLKFNYIKQDSVEPSWHYY
                     WLSEEGTQQLRPAIYSARIKVPDDLCGGDNAQRFELLRGYLQKVVAAHKETNPFDRLI
                     HFAGEGYNSDCLTAWRQYALVFSEYFPLAFASASGNTFLNFRQDPVIKYLLYDQIQRP
                     GTDLLAFYEHGAPDTQYINGDYPAQTFKENIGWLKHLLRVQYKRCKNPEDQQEFIKMN
                     CDMYHLDPIMFHPDTLTAYAVKDSSDEADRYIVLEDLKKLKPGARMVMLNACYNGSFH
                     EEGYVAGSYLFVPGSQTVTAQGNTVNVLQDKFADQLIGYMGMGIRLGFWQKEVVTLES
                     HMLGDPTYRFTACDRADEWNRALASGAPEAYWRGFLQAGEPMQRAMAIKQLTGMGCMT
                     SAGLEEIFTRDDSYIVRLQALVLSAKFADEHTKGIIMRGFHDPYENIRRQACNMAGKM
                     GCNDFIEPLKSLQAGAYETQRVQYAAQTALEVFDPALVGGGAELANPNLDEAGIRYLR
                     NNPQHFRIPELLGFLADAAQPADLRVVMAEALGWFNNSAQRMQIAGALEEQLRQKGLP
                     EALRAEMVKTIKRLKNN"
     gene            1800..>3623
                     /locus_tag="PLM68_08030"
     CDS             1800..>3623
                     /locus_tag="PLM68_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT55340.1"
                     /translation="MKKLKMWIPFVTLLFLLAGTFTLQAQRRQGVTLEGYVYEMDEDQ
                     KLPVPYASVSIASLSIAVTTDENGHYVINNLPARNLQVTARFLGLEERTIEVDLSKQQ
                     VLDFVLLVSNFRLEEIVVTAESNKAGAATASKINKTAMEHMQLTSLADVMSLLPGAAT
                     VKSDLTSVTTASIRGGSSLGTAILMDGAPISNNANMQILSSAIGGSTPSTSGTTPSIG
                     VDLRTITTDNIESIEVIRGVPSVEYGDITAGAIIVNSKTGRQPLSIKFNTNPNLYQGT
                     ITHGVNLGKNAGSLNYSGDYSYSVSDPREGYDFFRRVTARIGYSNTIGKWYTNTSVSF
                     LSALDKGEPNPDDEQDVTIQRQNDLGIRANTRGTFTFNRNFFKSMEYNIQFAYTDRDS
                     YFYDEATNATVAYSLSKHDGAVLSSIHGNKIYDVNGNLVTNVPAGEEYSIAWTLPSQY
                     KYDYTVDGRELNTFAKVKANFAGDLGPTSHRMVIGADFKSDGNIGEGKIFDPLNPPYR
                     SVSYTDATQRERSYKEIPFVNQFSLFAEENFDWEFASRHLQISAGIRYDHVLNFGGGF
                     SPRVNASIDILPKALTLNAAFGITRKAPTLAYLYPDKAYFDL"
BASE COUNT          958 a          966 c          926 g          773 t
ORIGIN      
        1 gctttcaaga tgaaccagaa ccgctttcca tggaacgaat gttccgttcc ttcggaccgg
       61 ttctacgatt gctttgattt gaaattcaat tacataaagc aagatagtgt tgaaccttcg
      121 tggcattact actggctctc ggaggaagga acgcagcagc tgcggccagc catttactcg
      181 gcacggataa aagtacccga cgacttgtgc ggtggtgaca atgcacaacg tttcgaactg
      241 ctgcggggct acctgcaaaa ggtagtggcg gcccataagg aaacgaaccc gtttgacagg
      301 ctcatacatt ttgcaggtga agggtacaat tcagactgcc tgacggcatg gcgccagtat
      361 gcgctggtct ttagcgaata tttcccactg gcatttgcct ctgcttcagg gaatacgttc
      421 ctcaatttcc ggcaggatcc tgtgataaag tacttgctat atgaccagat ccagcggccg
      481 ggcacagacc ttctggcttt ttatgaacac ggggccccgg acacacagta catcaacggg
      541 gattacccgg cccaaacttt caaagaaaat attgggtggc tcaagcactt gttgcgcgtg
      601 cagtacaaac gatgcaaaaa cccggaagac cagcaggaat tcattaagat gaactgcgac
      661 atgtaccatc tggatcccat catgttccat ccggacacgc tgacggccta tgccgtgaag
      721 gattccagcg atgaagccga ccggtacatt gtcctggaag atctgaaaaa actcaaaccc
      781 ggagccagga tggtcatgtt gaatgcctgt tataacggat cattccacga agagggatat
      841 gtagcgggtt cttacctttt tgtgcccgga agtcaaacgg ttacagcaca gggcaatacg
      901 gtgaacgtgc tgcaggacaa gtttgcagac cagctcattg gttacatggg tatgggcatc
      961 cgtctgggat tctggcagaa ggaagtggta acgctggaat cgcacatgtt gggcgacccc
     1021 acataccgtt tcacagcctg tgacagggca gatgaatgga accgtgcact ggcttcagga
     1081 gctcctgaag cctactggcg cggattcctt caggccggcg aacccatgca acgggccatg
     1141 gcaatcaagc aattgacggg aatgggatgc atgacctctg ccggactgga agagattttc
     1201 accagggatg actcctacat tgtcagactg caggcacttg tgttgtcggc aaaatttgcg
     1261 gacgaacata ccaaaggaat cattatgcgc ggtttccacg acccgtatga aaacatacgc
     1321 cgccaggcat gcaatatggc aggcaaaatg ggttgcaacg atttcattga accgttgaaa
     1381 tcactccagg ccggggctta tgaaacacaa cgcgtgcagt atgcggctca aacagcactg
     1441 gaggtctttg acccggccct ggtgggaggt ggtgccgaac tggcaaatcc taaccttgat
     1501 gaagcgggaa tccgctatct gcgcaacaat ccgcagcatt tcaggatccc ggaacttctc
     1561 ggttttcttg ccgatgctgc acaaccggcc gatttgcgtg ttgtcatggc cgaggcgctt
     1621 ggctggttca ataactcggc acaaagaatg cagatagccg gagcgctgga ggaacaactc
     1681 aggcagaaag ggctgcccga agcgctgcgg gccgaaatgg tgaaaaccat aaaacgcctg
     1741 aaaaacaatt aaccaacaac atatatattt tgcaataacc taagcacaaa acaagagtaa
     1801 tgaaaaaact taagatgtgg attccattcg taacgctgct tttcctgttg gccggcactt
     1861 tcacgctgca ggcacagagg aggcagggcg taaccctgga agggtatgtc tacgaaatgg
     1921 atgaagacca aaagcttcct gtgccgtatg cctctgtgag cattgcttcg ttaagcatag
     1981 cagtgactac agatgaaaac gggcactatg tgatcaacaa cctaccggcg cgtaatctgc
     2041 aggtcacagc ccgattcctg ggcctggaag aacggaccat agaggttgac ctatcaaaac
     2101 agcaggtgct cgatttcgta ctgctggtct cgaacttcag gctggaagaa attgttgtca
     2161 ccgccgagtc caacaaggca ggtgccgcca cggcatccaa gatcaacaaa acggccatgg
     2221 aacacatgca gctgacctca ctggccgatg tcatgtcgtt actgcccggt gctgccaccg
     2281 tgaagtccga tctgacctct gtcaccaccg cttcaatccg cgggggcagc agcctgggaa
     2341 cggccatcct gatggacggg gcgcccatat cgaacaacgc caacatgcag attctttcat
     2401 cggccattgg cggatcgaca cccagcacct ccgggaccac gccttccata ggtgtggacc
     2461 tgcgtaccat cactacagac aacattgaat cgattgaggt tatccgagga gtgccttccg
     2521 tagaatatgg ggatatcacg gcaggggcca tcattgtgaa ttcaaagaca ggccgccagc
     2581 cgctgtccat caagttcaac accaatccca acctgtacca gggaaccatc acccacgggg
     2641 tgaacctggg taaaaacgcg gggagcctga actatagcgg cgactattcc tacagcgtct
     2701 ccgaccccag ggaaggatat gattttttcc gccgtgttac agcccggata ggctattcca
     2761 acaccatcgg caaatggtac accaacacct cggtgagttt cctctcggcc ctggacaaag
     2821 gagaacccaa tcccgatgac gagcaggacg tgaccataca gcgtcagaac gacctgggta
     2881 tccgcgccaa cacaagggga accttcacat tcaaccgcaa cttcttcaaa agcatggaat
     2941 acaatataca gtttgcctat acagaccgcg acagctactt ctatgacgag gccaccaatg
     3001 caacggtagc ctattcgttg tccaaacatg acggcgctgt tctttccagt atccatggca
     3061 acaaaatata cgacgtgaac ggcaacctgg taaccaacgt gccagccggt gaggaatatt
     3121 ccattgcctg gacgctgcct tctcaataca agtatgatta tactgttgac ggaagggagc
     3181 tcaatacctt tgccaaggta aaagccaatt tcgccggaga cctgggcccg acaagccacc
     3241 gcatggtgat cggggccgat ttcaagagtg acggcaacat aggggaaggg aagatattcg
     3301 acccgctcaa cccgccatac cgctctgtaa gttacaccga tgccacacag cgtgaacgtt
     3361 cctacaagga aatcccgttt gtgaaccaat tcagcctttt tgctgaggag aattttgact
     3421 gggaatttgc cagccggcac ctgcaaattt ccgcaggtat ccgctatgac cacgtgctga
     3481 attttggcgg cggtttctct ccccgcgtga atgcatccat tgatatcctt cccaaggcac
     3541 tgacgctgaa tgcagccttc gggatcaccc gcaaagcacc caccctggca tacctttatc
     3601 cggacaaagc atactttgac ctg
//
LOCUS       DAOSOF010000164         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_25810_length_3614_cov_4.950267, whole genome shotgun sequence.
ACCESSION   DAOSOF010000164 DAOSOF010000000
VERSION     DAOSOF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25810_length_3614_cov_4.950267"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLM68_08035"
     CDS             complement(<1..317)
                     /locus_tag="PLM68_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOT55341.1"
                     /translation="MNDIKFQTKINSEIGELEAVILHTPGPEVENMTPRSVERALYSD
                     ILNLSVALQEYEQLSMFLEKITKVYQVKDLLVKVLDNLAQRNSLIGKICLTENVNDYY
                     DEL"
     gene            complement(314..1405)
                     /locus_tag="PLM68_08040"
     CDS             complement(314..1405)
                     /locus_tag="PLM68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOT55342.1"
                     /translation="MTRNQYCIIMAGGIGSRFWPLSRNAMPKQFLDILGLGKSFLQLT
                     YERFLRIIPSERILVVTSEQYKDLVRKQLPEILETNILTEPYRRNTAPCIAYATYKLL
                     KTDPQAIVVVAPSDHLITGEEDFLGTIRCALQLAAEQDVLITLGITPSRPETGYGYIQ
                     AIKNEPIAIGDHSAYPVKTFTEKPDAEMARILVDSGEFYWNSGIFVWSLASIVRQLER
                     WLPEVSLPFRNGISLYNTPHEEKFIKKLYEEIRNVSIDYGVMEKAKNTCVFSATFGWS
                     DLGSWESIYMHQDKDTRGNAVSATSSLLESVKDSFVYSCNQGKLLVVKGLENYIVVDT
                     PDALLICPRDETQFRQLFNDIAHNHEEFV"
     gene            complement(1402..1791)
                     /locus_tag="PLM68_08045"
     CDS             complement(1402..1791)
                     /locus_tag="PLM68_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT55343.1"
                     /translation="MEGKKGNTLEKGRKGEALAGEFLKREGFRIFHTNWRTGHKELDI
                     VAGKDGCIHFVEVRSRSRGSVVSPEWTVDRYKQRKIMAAARAYMAKYHITDEARFDVI
                     AIVLGTGGTDYQDYELTYIPNAFTLIL"
     gene            complement(1827..3392)
                     /locus_tag="PLM68_08050"
     CDS             complement(1827..3392)
                     /locus_tag="PLM68_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOT55344.1"
                     /translation="MKQVTGHIALFLLILSISCTKQPVSLLILQTSDLHGHFDESVAG
                     LAGYVREQQQLYGKNLLLFDTGDNLQGTPGLYYYSHVDTAEKHLCAAFFDWFPYTAVT
                     VGNHDIEAGINVFSRVYRQTEAPVLAANILHEMTGETYFRPYRIFHRKGYKIAVLGMI
                     TPKVTSWVPEWLRPGLDFDPMTASMQYWVPLIHEKEKPDVLIGLFHTGAGSKEGKTAG
                     KENAALWIAENIPGFDLICYGHDHKPMVQYAINKENDTVWLMNPGAYGKQLARAEIIL
                     NSGARCKAQIKAALVDVSHLSPDLDYLSGFEPYFRQAAAYEQQPIAELLNTMESNLVF
                     EGPSRWLDEVHRGQLELAGEDPGIRADVSFASALRSDVVIEKGPLDLKDFFAWFPFEN
                     SLCILEMTGWEIRQYLEYSYDQALIINFDTAAGILYTVCRNKPEGERIKIHSMADGTA
                     FDTGKRYHVVMNSYRAQGGGGHLSKGLGWDNATISSRVIWDSRKDMRTLFMQWEASRS
                     PISADPLNHWNYE"
BASE COUNT          851 a          921 c          827 g         1015 t
ORIGIN      
        1 atcagctcgt catagtaatc gttcacgttt tctgtaaggc atatcttgcc tatcagggaa
       61 ttccgctgtg ccaggttgtc cagaaccttc accagcaggt cctttacctg gtagaccttt
      121 gtgatctttt ccaggaacat ggacaattgt tcgtactcct gcagtgccac cgaaaggttc
      181 aggatgtcac tgtacaatgc ccgttccaca gacctggggg tcatgttttc cacttccggt
      241 cccggagtgt gcaggatcac ggcttccagt tcccctatct cggaattgat ctttgtttga
      301 aatttaatgt cattcataca aattcctcat ggttatgtgc aatatcgttg aacaactgac
      361 ggaattgggt ctcatccctg gggcagatca gcaatgcgtc aggtgtgtca accactatat
      421 aattttccaa acctttgaca accagtagct tcccctggtt acaagaatac acgaaactgt
      481 ctttgacact ttccagcagg gagcttgtcg cagatactgc attgcccctt gtatccttat
      541 cctggtgcat atagatactt tcccagcttc ccaggtcgga ccatccgaat gttgctgaaa
      601 agacacaggt gttctttgct ttttccataa ccccgtaatc tatggataca ttgcggatct
      661 cttcatacaa tttcttgatg aatttctctt cgtgcggagt attgtacaag cttataccgt
      721 tacggaaggg aagactgact tccggcaacc agcgttccag ctgccggaca atagaagcca
      781 ggctccagac gaaaatcccc gaattccagt aaaattcacc actgtcaacc aggatgcgtg
      841 ccatttcggc atcgggtttt tcggtaaatg tttttacggg atatgcgcta tgatctccta
      901 tggctatagg ttcattcttg atagcctgta tataaccgta accggtctcg ggacgggaag
      961 gcgtaatacc cagggttatc agcacatctt gctctgcagc cagctgcagg gcacaacgga
     1021 tggtccccag aaagtcctcc tccccggtaa tcaaatgatc ggaaggagct accaccacga
     1081 tagcctgggg gtcggtcttg agcagcttgt aggtagcata ggcaatacag ggtgccgtat
     1141 tgcgacggta tggttcggta aggatgttgg tttccaggat ttccggtaat tgtttacgga
     1201 ccaaatcttt gtattgttcc gatgtaacca ccagtatcct ttcggagggg attattctca
     1261 ggaacctttc ataggtaagc tgcaggaaac tctttcccag gcccaggata tccaggaatt
     1321 gtttgggcat ggcattccgg ctcaggggcc agaaacgcga tcctatgcca cctgccataa
     1381 taatgcaata ctgatttctg gtcataaaat aagtgtaaaa gcgttgggaa tatatgttaa
     1441 ttcataatcc tgatagtctg ttccaccggt tccaagcact atggctatga catcgaaccg
     1501 ggcttcatca gttatatggt acttggccat ataggcgcgg gcagctgcca ttatttttcg
     1561 ttgtttgtac cggtcgacag tccattcggg agacacaaca ctgccgcggg aacgtgatct
     1621 tacttcaacg aaatggatac atccgtcttt cccggcaact atatccagtt ctttatggcc
     1681 tgttctccag tttgtatgaa agatcctgaa tccttctctt tttaaaaatt ccccggccaa
     1741 tgcctctcct tttctcccct tctccagggt gttccctttc tttccctcca tatcgtttcc
     1801 cctgtgacaa atgtaggcaa tttttattat tcgtaattcc agtgattgag aggatctgcg
     1861 gaaatcgggc tgcgggaagc ttcccactgc atgaagagtg ttctcatgtc tttcctgcta
     1921 tcccaaatca ccctgctcga tatggtggcg ttatcccatc ccagtccttt tgataagtgg
     1981 ccccctccgc cctgcgcacg ataagagttc attaccacat ggtatctctt tccggtgtca
     2041 aatgccgtac cgtctgccat actgtggatc tttatccttt ctccctcagg tttgttccgg
     2101 catactgtgt acagaatacc ggccgcggta tcgaaattga tgatcagggc ctggtcatat
     2161 gaatattcaa ggtattgtct tatttcccat cctgtcattt ccagtatgca taaggaattt
     2221 tcaaacggga accatgcaaa gaaatctttc aggtcaagcg ggcccttttc aatcaccaca
     2281 tcggaccgta aggccgaggc aaaggacaca tcggcccgga taccgggatc ctctcctgcc
     2341 agttcaagct gacccctgtg cacttcgtcc agccatcggg atggtccttc aaaaaccaaa
     2401 ttgctctcca tggtgttcag cagttctgct atgggctgtt gttcataagc ggctgcctgc
     2461 ctgaaataag gttcaaatcc gctcaaatag tccagatcgg gtgacagatg gctaacatcc
     2521 accaatgctg ccttgatctg ggccttacaa cgggcgccgc tattcaggat tatctcggcc
     2581 cgggccagtt gcttaccgta agcccctgga ttcatgagcc atactgtatc gttttccttg
     2641 ttgatagcgt attggaccat tggtttatgg tcgtgtccat aacaaatcag atcaaagccg
     2701 ggaatgtttt ctgctatcca cagtgctgca ttctcttttc ccgcggtttt cccctccttg
     2761 ctccctgcac cggtatggaa taaacctatg agcacatcgg gtttctcttt ctcatgtatc
     2821 aaagggaccc agtactgcat agaggctgtc atggggtcaa agtccagacc gggacgcagc
     2881 cactccggga cccaggatgt cacttttggt gtgatcattc ccaggacggc gatcttgtaa
     2941 cctttcctgt gaaaaatacg gtagggtctg aaatacgttt ccccggtcat ttcgtgaagt
     3001 atgttggcag caagcaccgg tgcttctgtt tgcctgtaaa ccctggaaaa cacgtttatt
     3061 cccgcttcaa tgtcatggtt tccaacagtc acggcagtat agggaaacca atcaaaaaag
     3121 gcagcacaaa gatgcttttc tgcagtatcg acatggctgt aataataaag ccccggggtg
     3181 ccctgcaggt tatcccccgt gtcgaagagc aacaggttct tcccgtatag ttgctgctgt
     3241 tcccggacat atccggccag tcctgccacg gactcatcaa agtgaccgtg caggtcgctt
     3301 gtctgaagga taagcaggga gacgggctgc tttgtacagg aaatgcttag aatgagcaaa
     3361 aaaagcgcaa tatgacctgt gacctgtttc attttcatta atacaaaaat aagaaaaaac
     3421 gcaactccgt cagccgggat ccgataaaac acccccggaa atcctttagt cctggatccg
     3481 ggggtgcatc agagatgcca tccttttcag gcgctatatc tctgtgagat ttggtttaat
     3541 gccggtgcgg ttattctgcg gctgcaggtt cgtgcgatac atagaaaccg ctgccaacct
     3601 ggggagttcc ttta
//
LOCUS       DAOSOF010000165         3533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_26595_length_3533_cov_4.402243, whole genome shotgun sequence.
ACCESSION   DAOSOF010000165 DAOSOF010000000
VERSION     DAOSOF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26595_length_3533_cov_4.402243"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLM68_08055"
     CDS             complement(<1..222)
                     /locus_tag="PLM68_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOT55345.1"
                     /translation="MKRVVITGIGIYSCIGVSKEEVRDGLYQGRSGIGIDPSRTEYGY
                     RSPLTGIVPRPDLKESLTRRQRICLPEEGE"
     gene            complement(232..960)
                     /gene="fabG"
                     /locus_tag="PLM68_08060"
     CDS             complement(232..960)
                     /gene="fabG"
                     /locus_tag="PLM68_08060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOT55346.1"
                     /translation="MKYALVTGGSRGIGRAICVALAKEGYSVIVNYARNREAAEKTVE
                     MIASEGGMAQLLQFNVASKEEVDNALDAWDNEHPGEYISVLVNNAGIRQDNLMIFMQD
                     EQWNNVLETNLFGFFYVTRRVLKNMLTRRYGRIVNVTSLSGLTGLPGQTNYAASKAGL
                     IGATRALAMEVAPRKVTVNCVAPGFIETDMTSDINQDEHKGRIPAGRFGKPAEVAAAV
                     VFFAGENASYITGQVLSVNGGLYT"
     gene            complement(965..2494)
                     /locus_tag="PLM68_08065"
     CDS             complement(965..2494)
                     /locus_tag="PLM68_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOT55347.1"
                     /translation="MILLNRELTLKNIEDLLFAKETVSLDKEAIDRITRSYEFLKEFS
                     KEKVIYGINTGFGPMSQFRVDDSNLKQLQLNLIRSHAAGTGEILTDLQVKAAMIARLA
                     TFVQGCSGVHPSLPVLLAECINRDILPVIPQHGSVGASGDLVQLAHMALALIGEGKVH
                     YHGQTRDAAEVLKENNLEPMQIHIREGLAMTNGTSVMTGIGLLNFFNARKLLLWAMSA
                     SALVNEIAGSYDDFLSPVLNGKKRHDGQLFIARQLSDMLSESRCLKRRQSFLYNREVN
                     GDKVFRDKVQPFYSLRCVPQILGPVADTLEYAGRVLLEELNSCCDNPVVDPETENVYH
                     GGNFHGDYVSLEMDKMKTVVTKMTMLMERQLNYICHDKVNQILPPFVNLGIPGLNYGM
                     QAAQFTATSTTAECQTLSMPNYIHSIPNNNDNQDVVSMGTNSGLITARVIENAFQVTS
                     IHLMALAQAVSCLDITEKLSPPTLTVYKRIRGIFPAVKEDVALYPYIAKTVEYITHNK
                     P"
     gene            complement(2535..>3533)
                     /locus_tag="PLM68_08070"
     CDS             complement(2535..>3533)
                     /locus_tag="PLM68_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT55348.1"
                     /translation="PLLPLDPGKIRSIAVIGPRADEVIFDWYSGTPAYTVTILEGIRN
                     AVGPDVEVLYEPSNTMDKAYIAASKADVAVVCIGNHPYGTNARWFYSPVPSDGREAVD
                     RKALTLEQEDLAKVVLKANPNTVLALVSSFPFTINWSEEHIPSIVHIANNSQELGNGL
                     ADVLFGKVNPAGRTTQTWVRDIADLPPMMDYNIRNGRTYMYFAGKPLYPFGHGLSYTT
                     FEYGSLKMPESFADSTQIAVKIRNTGSFDGEEVVQLYVEYPSSRIARPNRQLVGFERV
                     MVPAGQERKVILTLKALDMAFWDVKKQRFAVEPGAVKVLVGSSSANIRLSGILEVK"
BASE COUNT          806 a          929 c          869 g          929 t
ORIGIN      
        1 ttcaccttct tcgggcaaac aaatcctttg cctgcgggtc agcgattctt tgagatcggg
       61 ccgtggaaca atgccggtga gcggagaacg gtaaccgtac tccgtcctgg atgggtcaat
      121 gccaatcccg cttcttccct ggtaaagacc gtccctgact tcttccttac tgacaccgat
      181 acatgaataa attcctattc ctgtaatgac tactctcttc atgctatcgt actaagtgta
      241 taatcccccg ttgacagaca aaacctgccc ggttatgtat gaagcgtttt caccggcaaa
      301 aaagacaacg gcagcggcta cttctgcagg ttttccaaaa cgtcctgccg gtatccttcc
      361 cttgtgttca tcctggttaa tgtcgctggt catgtcggtc tctataaaac cgggagccac
      421 acaattgacg gtgactttcc ttggagccac ctccatggcc agggcacgtg tagccccgat
      481 aagaccggcc ttggaagctg cataattagt ctgtccaggc aagcctgtca gacccgaaag
      541 cgaggtcacg tttacaatcc tgccgtaacg tctggtcaac atgttcttta gcaccctgcg
      601 ggtaacatag aaaaaaccaa acaaattggt ttccagtaca ttgttccact gttcatcctg
      661 catgaaaatc atcaggttgt cttgcctgat acccgcgttg tttacaagaa cgcttatata
      721 ttcccccgga tgttcattat cccatgcatc cagggcattg tccacttctt ccttgctggc
      781 aacgttgaat tgcagcaact gcgccatacc cccttccgaa gctatcattt ccacggtttt
      841 ctctgctgct tcccggtttc ttgcataatt gactatcacg gaatatccct ctttagccag
      901 ggcaacacat atagcgcgtc ctatgcctcg tgagcctccg gttaccaaag cgtacttcat
      961 gatatcatgg tttattgtgg gttatgtatt ctacagtctt ggcaatatag ggatacagtg
     1021 ccacatcttc tttcacagcc gggaagatcc ctcggatcct cttatagaca gtcagtgtgg
     1081 gcggagagag tttttccgtg atatccaggc aggaaaccgc ctgtgccagg gccatcaggt
     1141 gaatggaggt cacctgaaag gcattttcaa tgacccgggc agttatcagg cccgagttgg
     1201 tgcccatgct taccacatcc tggttgtcgt tgttgttggg gatggaatgg atgtagttgg
     1261 gcatgctaag agtctggcat tctgctgtag tggaagtggc ggtgaactgg gcagcctgca
     1321 ttccgtaatt cagaccgggg atgcccaggt tcacgaaggg cggcaggatc tggttgacct
     1381 tgtcatggca tatgtaattg agctgccttt ccatgagcat ggtcatcttg gtgaccacgg
     1441 tcttcatctt gtccatttcg agcgaaacgt aatctccatg gaaatttcct ccatggtaaa
     1501 cattttcggt ttcgggatcc accaccgggt tgtcgcagca agaattcaat tcttccagga
     1561 gcacacgccc ggcatattca agtgtgtcgg ccacaggacc caggatttgc ggaacacagc
     1621 gcaaagaata aaatggctgc accttgtccc ggaatacctt gtcaccgttg acttccctgt
     1681 tgtacaggaa agattgcctt cttttaagac acctgctttc gctgagcatg tcagataact
     1741 gccgggcaat gaacaactga ccgtcatggc gttttttgcc gttaagcaca ggagacagga
     1801 agtcatcata ggagccggca atttcattga caagagccga ggcggacatg gcccacagca
     1861 acagcttcct ggcattgaag aaattcagca gcccgatgcc cgtcatcaca gaagtcccgt
     1921 ttgtcatggc cagtccttcc cgtatatgta tctgcattgg ctccaggttg ttttctttca
     1981 gcacttcagc agcatccctg gtctggccgt ggtaatgtac ctttccttcg cctatcaggg
     2041 ccagtgccat gtgggcaagc tgcacaaggt ctccgcttgc acccacactg ccgtgctgtg
     2101 gaataaccgg caatatatcc cggttgatgc actctgccag caacacaggc agggagggat
     2161 gcacgccgga acaaccctgg acaaaagtgg ctagtcgtgc aatcatggca gccttgacct
     2221 ggaggtccgt caggatctcc ccggtgccgg ctgcatgcga acgtatcagg ttcagctgaa
     2281 gctgtttcag gttgctgtca tccaccctga attgcgacat gggaccgaat cccgtgttga
     2341 tgccgtatat gactttttct tttgaaaatt ctttcaggaa ttcatacgaa cgggttatcc
     2401 tgtcaatggc ttccttgtcc agtgatacgg tttctttcgc gaatagaagg tcttctatgt
     2461 ttttcagggt taattccctg tttaataaga tcataaaaaa gatagcacta aatcaagctg
     2521 caaagatact ttttttattt cacttcaagt attccggata aacggatatt tgcagatgaa
     2581 ctgcccacaa gaaccttcac tgcgcccggt tcaactgcga accgttgttt cttcacatcc
     2641 cagaaagcca tgtccagggc tttgagtgta aggataacct tacgttcttg cccggcagga
     2701 accatgacac gttcaaaacc cacaagctgc cggttcggac gggctatcct ggaagaggga
     2761 tattcaacat acagctgcac cacttcctca ccgtcaaagg agcccgtgtt cctgatcttc
     2821 actgctatct gtgtgctgtc agcaaagctt tcaggcattt tgagcgaacc atattcaaag
     2881 gtggtatagc tcaggccgtg tccgaagggg tatagcggtt tccctgcaaa gtacatgtag
     2941 gttctaccgt tgcggatatt gtaatccatc atgggaggca ggtcggcaat gtccctgacc
     3001 caggtctgtg ttgtccttcc tgcaggattc actttcccga acaggacatc ggcaagtccg
     3061 ttgcccagtt cctgtgaatt gttcgcaata tgcactatgg acgggatgtg ttcctcggac
     3121 cagttgatgg taaagggaaa gctgcttacc agagccagca ccgtattcgg attggccttc
     3181 aggaccactt tggccaggtc ttcctgctcc agggtgagcg ccttgcggtc aacggcttcc
     3241 cgtccgtcgc tggggaccgg cgaatagaac caccgggcgt tggtgccgta gggatggttg
     3301 cctatgcata ccacggccac atcggctttg gaagccgcaa tatatgcctt gtccatggta
     3361 ttggacggtt cgtataatac ttccacatcc ggcccgacgg cattacggat cccttccagg
     3421 atggttacag tataggccgg tgtgccgctg taccagtcaa agatcacctc atcggcccgt
     3481 ggacctatga cggcaatgga acgtatcttg cccggatcca ggggaagcaa ggg
//
LOCUS       DAOSOF010000166         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_26757_length_3516_cov_4.393817, whole genome shotgun sequence.
ACCESSION   DAOSOF010000166 DAOSOF010000000
VERSION     DAOSOF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26757_length_3516_cov_4.393817"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLM68_08075"
     CDS             complement(<1..494)
                     /locus_tag="PLM68_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55349.1"
                     /translation="MIDFHDALQIVMGHATPAGTEMISLEDACGRILSLDIKAPADMP
                     PFSKSAMDGYACRKEDTENGMAVLEVITAGKLPVHEIIPGMCSKIMTGAIMPEGANAV
                     LILENAKVRDGRVFGPRPASDNIIRQGEDLKAGDPVLHAGTLMQPQHVSLIASCGMTK
                     VAVSR"
     gene            543..1337
                     /locus_tag="PLM68_08080"
     CDS             543..1337
                     /locus_tag="PLM68_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55350.1"
                     /translation="MMFLRLNNTTTRLVFLFLSGILLLFIVAPLLGLFLSTSLPDLFE
                     TIKDTDVQRSIGLTLGVSALFTLLAGFFALPLAYIMARKEFRGKAFIQGLIDIPIVLP
                     HSAAGIAVLGFISRDSALGRLADIMGLNLVGSPFAIGVAMAFVSVPFLINAARDGFEA
                     VPERLEQAARSLGASPFRVFWQISLPLAKPSVITGLVLMFARGMSEFGAVVIVAYHPM
                     TTPVLIFDRFNSFGLDYARPVAVLFIIICILVFMALRLLGRNKKKK"
     gene            1339..1956
                     /locus_tag="PLM68_08085"
     CDS             1339..1956
                     /locus_tag="PLM68_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT55351.1"
                     /translation="MLLCRDLEYKVGTFHLEGFSLVLQQGDYCALLGPSGSGKTIILE
                     LIAGLRTLKKGSIIIDGKDCTLLPPQRRPVGILFQDYALFPHMSVFENIAWPLEIRKM
                     KKNEIKRTVVSFASDLDIDRHMDRMPGSLSGGEKQRVALARTLVVHPKVLLLDEPLSA
                     LDAPLRDQSVLLLKKVSQTGITILHVTHDQAEVRFLANRQITIGS"
     gene            complement(2203..3141)
                     /locus_tag="PLM68_08090"
     CDS             complement(2203..3141)
                     /locus_tag="PLM68_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT55352.1"
                     /translation="MKKQIRKLPLLTLRRLLLSSKLLLLPLLGISFSGCSQKSDKQLV
                     VFHAGSLSLPMKALADSFATIHPGTEFLMEAAGSVDCARKITELGRSCDIMASADYMV
                     IDQLLIPEYASMNQPFAMNSIVIAFTDKSRKSDEINSENWYRVLLSPDVFYGRSDPNA
                     DPCGYRTMFTLQLAERHYAGSGSKVPFRVSDFTGKDHRFIRPKEVDLLALLGSQAIDY
                     MFIYKSVAMQHGLRFLELPPEINLGDSTMANLYASAQLKIRGGSPGDSVLVRGTPITY
                     SFTIPHNARNPELAKKFADFLTDPDGGQKILRSMGQ"
     gene            complement(3143..>3516)
                     /locus_tag="PLM68_08095"
     CDS             complement(3143..>3516)
                     /locus_tag="PLM68_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55353.1"
                     /translation="DTLAVITRPDSELPVITYPVLFYGNSTGYKGVREFSGQMLSRMI
                     QPWFPSDDYKTVQTGMLSIAGVDGFRAAFSLAEVVNRNDNREILLMQRGKSFSLYTAP
                     DAFADRCIKGLSEIRLIDPEE"
BASE COUNT          791 a          894 c          928 g          903 t
ORIGIN      
        1 cggcttacag cgacttttgt catgccgcat gaagctatca atgatacatg ttgcggttgc
       61 atcaacgtcc cggcatgcag gaccggatca cctgccttca ggtcttctcc ctgacggata
      121 atgttgtccg atgccggtcg cggaccgaat acccgtccgt cacgcacctt ggcgttttcc
      181 aggatcaaca cggcattggc gccttcaggc atgatggccc cggtcatgat cttgctgcac
      241 atgcctggaa tgatctcatg aacaggcaat ttgccggcgg taatcacttc caggacagcc
      301 atcccgtttt ctgtatcttc cttgcggcag gcatatccgt ccattgccga ttttgaaaag
      361 ggaggcatat cggcaggggc ctttatatcc agggaaagga tacgtccaca ggcatcttca
      421 agtgatatca tttcagtgcc ggcaggggtg gcgtggccca tcacaatttg caaagcatca
      481 tggaaatcta tcatagaaac aaagatacaa tggatttttt tatatttgct ttgtaaagaa
      541 agatgatgtt cctcaggctc aataatacga ccacacgcct ggtcttttta tttttatccg
      601 gtatcctgct gctgttcatt gttgcaccgc tgctggggct tttcttatcc acatccctgc
      661 cggatctgtt tgagacaatc aaggacacgg atgttcagcg aagcattggc ctcacgctgg
      721 gcgtctcggc gctttttacc cttctggctg gttttttcgc ccttccgctg gcctatatca
      781 tggctcggaa agaattcagg ggaaaagcct tcatccaggg attgatcgac atacccatcg
      841 tactgccgca ctctgcggcc gggatagctg ttctggggtt tatttcaagg gactctgccc
      901 ttggcaggtt ggcagatatc atgggactga acctggtggg cagtcccttc gccatcgggg
      961 tagccatggc ttttgtaagt gttccttttc tcatcaatgc ggccagggat ggatttgagg
     1021 ccgtaccgga acgcctggaa caggctgccc ggtccctggg agcttcacct ttcagggttt
     1081 tctggcagat atcccttccg ctggccaaac catctgtgat tacagggctt gtgcttatgt
     1141 tcgccagggg aatgagtgag ttcggcgccg tggtcatagt tgcttaccat cccatgacca
     1201 cccccgtgct gatatttgac cggttcaatt ccttcgggct tgattatgcc cggccggttg
     1261 ctgtgctttt tattatcata tgcatcctgg tgttcatggc gcttcgcctg ttgggccgaa
     1321 acaaaaaaaa gaagtgatat gttgttgtgc agggacctgg aatataaagt gggcactttt
     1381 cacctggagg gattctctct ggtgcttcaa cagggcgatt actgtgccct gttgggcccg
     1441 tccggatctg gcaagacaat catcctggag ttgatagccg gattgagaac cctgaaaaaa
     1501 ggctcgataa tcattgatgg caaggattgt accctccttc ccccgcaacg cagaccggtg
     1561 gggatcctct tccaggacta cgcgctcttt ccgcacatga gcgtctttga aaacatcgcc
     1621 tggcccctgg agatcaggaa aatgaaaaag aatgagatca aaaggacggt ggtcagcttt
     1681 gcctctgacc tggatattga taggcacatg gacaggatgc ccgggtcgct ctccggaggg
     1741 gagaaacagc gggttgccct agcccgtacg ctggtcgttc atcccaaagt gcttttactg
     1801 gacgagccct tgtccgcgct ggatgccccg ctaagggacc aatcggtcct tctactgaaa
     1861 aaggtgtcgc agactggcat caccatcctt cacgtcacgc atgaccaggc cgaggtgaga
     1921 ttcctggcca accgccagat caccatagga tcataacact ccggcttttt gctccggggt
     1981 accattcttt tgctgcctgc tgcggccgct gacatcacac gaacgggaag cttattcatt
     2041 aagccgaggc tctacgttcc aaggggtggc agtgaaagaa gcaggcagca tatgcaggaa
     2101 agcttcggca taaagtaaaa gtgctctgtt ccatgtagtc tcagcgagca ttacaggtac
     2161 caacagaata agtgaagctt atcggtgtat aattaaggta cgttactgtc ccatggagcg
     2221 cagtatcttc tgtcctccgt cgggatctgt caggaaatct gcgaattttt tcgccagttc
     2281 aggattccgg gcattgtggg ggatggtgaa actgtaagta atgggggttc ctcttaccag
     2341 gacggagtcc ccggggctgc cacccctgat tttcaactgt gccgaggcat agaggtttgc
     2401 catagtgctg tcacccaggt tgatttccgg gggcaattcc aggaaccgca ggccgtgttg
     2461 catggcaact gatttataga tgaacatata gtctatggcc tggcttccca gcaatgccag
     2521 caggtccact tccttggggc ggatgaaacg gtggtctttt cctgtaaagt cacttacccg
     2581 gaaagggacc ttgctgccgg atccggcata atgacgttct gccagctgca aggtgaacat
     2641 ggtacggtat ccgcaggggt ctgcatttgg atctgatcta ccatagaaca catcagggga
     2701 aagcaatacc cggtaccagt tctccgaatt aatctcgtcc gatttgcgcg atttgtccgt
     2761 gaaggcaatg actatgctgt tcatggcaaa gggttggttc atggatgcat attcaggtat
     2821 cagcaactgg tcaatgacca tgtaatctgc cgaagccatg atgtcacagg atcgtcccag
     2881 ctctgtgatc ttgcgggcgc aatccacgct cccggcggct tccatgagaa attcagttcc
     2941 gggatgtatg gtggcaaaac tgtcagccag ggctttcatg gggagtgaca ggctgcctgc
     3001 gtgaaagaca accagttgct tgtcagattt ttggctgcaa ccggaaaatg atatgcccag
     3061 aagcggcaag agcagcaatt ttgacgacag aagtaatcgt ctcaaagtca gcaaaggcaa
     3121 cttacgaatt tgttttttca tatcactctt ccgggtcaat caaccggatt tcactcagtc
     3181 ctttgatgca tctgtctgca aaagcatcgg gggctgtata cagggaaaaa gattttcccc
     3241 tttgcataag gaggatttcc cggttgtcgt tccggttgac cacctcggcc agggaaaaag
     3301 ccgcccggaa accatccaca ccggcaatgc tcagcattcc tgtctgcacc gttttgtaat
     3361 cgtcagaggg gaaccatggc tggatcatcc tgctgagcat ttgtcctgaa aattcgcgga
     3421 ctcccttgta acctgtgctg ttaccgtaaa aaagcacagg ataggtaatc acaggaagtt
     3481 cgctgtccgg ccgggtgata acggccagcg tatcct
//
LOCUS       DAOSOF010000167         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_27053_length_3487_cov_5.216492, whole genome shotgun sequence.
ACCESSION   DAOSOF010000167 DAOSOF010000000
VERSION     DAOSOF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27053_length_3487_cov_5.216492"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLM68_08100"
     CDS             <1..764
                     /locus_tag="PLM68_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT55354.1"
                     /translation="LNQAQRLFDRTQELFKAGVVVREEFENVETQLQLSKEELSNAQD
                     HLEIVRDGIASRSAQASNTQIRSTISGMILDVPVKVGNSVIQANTFNAGTTIATVADM
                     NDMIFRGNIDETEVGRIREGMPLDITIGAIQDLHFDAVLEYISPKSTENNGAILFEIK
                     AAVDVPDTVFVRAGYSANAGIILDSSYDVLVIPESSVVFRDGGTFVNVFKGLEGDKQV
                     FEEKPVVLGLSDGIQVEIKEGLTTEDQVRGSLVTE"
     gene            777..2129
                     /locus_tag="PLM68_08105"
     CDS             777..2129
                     /locus_tag="PLM68_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT55355.1"
                     /translation="MRKIMILCAGLLFSSGLLAAQTPDKSWTLDDCIRYGLENNITVK
                     QYELAKENQEITVETTRFSRLPDLSANVGQTFYFGRTPDRDGVYQDQTGSNSSLGINT
                     SVNLFSGFRVTNQIKAEKLELQAAVEDLSRAREDLSLSITGNFLQVLLSRELYQISLD
                     QLELNRRQVEQTQFLVTGGKSSESDLFDARSSLAQQQMQVTDAANNLQLSLLALTQAM
                     NLPHVEGFNVATPDMEEFTRREALGLEQPSGVYENSVKERPAIKAAAFRLEGSEKVLQ
                     AARSAFYPSLRLGAGYSNSYYFNYSLAAGLSNASLADQLAQNSAQSVGLSLSIPIFNR
                     MATRNQVRSARLNIKNQELMLDKAKQDLYKEVQQAYYNAVAALEKFKTSEVAVEAAQL
                     AYDFEEQKYEAGRSNIYTFDQVRLRLSTARSESAQAKYNFLFRARILAFYNGESLGGD
                     "
     gene            complement(2406..2702)
                     /locus_tag="PLM68_08110"
     CDS             complement(2406..2702)
                     /locus_tag="PLM68_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55356.1"
                     /translation="MKALFISFYQAFYDEVMKALEKNHVKGFTFWEEVRGHGSRDGEP
                     HLGSHAWPTLNSALITFVPDHVVDQLLQDLKKADESAPKQGLRAFVLEAEERTV"
     gene            complement(2699..>3487)
                     /locus_tag="PLM68_08115"
     CDS             complement(2699..>3487)
                     /locus_tag="PLM68_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT55357.1"
                     /translation="EDIQNILIYNNQGQKIRLRDVAAIGETMQPPTIERKDRERIVTV
                     TCVAAKGAVLSDLVAAARQQIEKVDIPPEVMVSIAGSYEDQQESFKDLGMMMILILIL
                     VYIVMAAQFESFSSPFIIMFSVPFAFTGVLAGLAISNTALGIMSMVGLLMLFGMVVKN
                     GIVLIDYIVLCRERGMSIREAVVTSGRSRLRPILMTALTTIFGMVPLAVGSGVGAEMW
                     NGLGITVASGLTVSTAVTLFLIPAIYSLTAERKEKRKMRKAQQL"
BASE COUNT          908 a          868 c          887 g          824 t
ORIGIN      
        1 acctgaacca ggcacagcgg ctttttgacc gcacccagga attattcaag gcaggggttg
       61 ttgtacggga agaatttgaa aatgtggaaa cgcaactgca attgtccaaa gaagagctca
      121 gcaatgccca ggaccacctg gaaattgtcc gtgacggtat tgccagccgt tctgcccagg
      181 caagcaacac acaaattagg tcaactattt ccggcatgat actggatgta ccggtgaaag
      241 tgggaaattc cgtgatacag gccaatactt tcaatgccgg aacaaccatc gccaccgtcg
      301 cagatatgaa tgatatgatc ttccggggta atattgatga aaccgaggtg gggagaatcc
      361 gggaagggat gcccctggat ataaccatag gagccataca ggatcttcat ttcgatgcag
      421 tactggaata tatatcgcca aaaagcacag agaacaacgg ggccattctt ttcgagatca
      481 aagcggcagt tgatgttccc gacactgtgt ttgtaagggc agggtacagc gccaatgccg
      541 ggatcatact ggacagcagc tatgatgtgc tggtcattcc cgagagttcc gtggtcttcc
      601 gggatggggg aacctttgtg aatgtgttca agggactgga aggtgacaag caggtgtttg
      661 aagaaaaacc cgtggtgctg ggccttagtg acgggatcca ggtagagatc aaagaaggcc
      721 tgaccacgga ggatcaggtg cgtggttcac tggttactga ataatacagg gaaattatga
      781 gaaaaattat gattttgtgt gcaggtctgc tcttttcttc cggcctgctt gcagcccaga
      841 ctccggataa atcatggacg ctggatgatt gcatccggta tggactggaa aacaatatta
      901 ccgtcaaaca atacgaactg gcaaaagaaa accaggaaat cacggtagaa accaccaggt
      961 tttcccgcct tcccgatctg tcggccaatg tgggccagac cttttacttt ggacgtacac
     1021 cggaccgcga cggcgtttac caggaccaga ccggttccaa ttcatccctg ggtatcaata
     1081 ccagcgtcaa tctattcagc ggattccggg ttacaaacca gatcaaggcg gagaaactgg
     1141 aacttcaggc tgcggtggaa gacctgagcc gtgcaaggga ggacctctct ctgtccataa
     1201 ccggtaattt cctgcaggta ttgctgagcc gggagttgta ccagatttca ctggaccagc
     1261 tggagctgaa ccgcaggcag gtggaacaga cccaattcct ggtaacaggg ggaaaaagtt
     1321 ccgaaagcga tttgttcgat gcccgttctt ccctggccca gcagcagatg caggtaaccg
     1381 atgcagctaa caacctgcaa ttatccctgc tggcacttac acaggccatg aacctgcctc
     1441 atgtagaggg ttttaatgtg gccacacccg atatggaaga gttcaccagg cgggaagcat
     1501 tgggactgga acaaccatcg ggagtttacg aaaattctgt aaaagaacgc ccggcgatca
     1561 aggcggctgc tttccgcctg gaaggaagcg aaaaagtgtt acaggcagcg cgcagtgcct
     1621 tttatcccag cttgcgcctg ggggctggtt acagcaattc ctattatttc aattattcgc
     1681 tggcagccgg attgagcaat gcatccctgg cggaccagct ggctcaaaac agtgcccagt
     1741 cagtgggact gagcctgagc ataccgatat tcaacagaat ggccacccgg aaccaggtgc
     1801 gttcggcccg cctgaatatc aaaaaccagg aattgatgct ggataaggca aaacaggatc
     1861 tctacaaaga agtgcaacag gcatactaca atgccgtggc tgcgcttgaa aaattcaaga
     1921 cttctgaggt cgcagtggag gctgcgcaac ttgcctatga ttttgaagag caaaaatatg
     1981 aagccggccg ttcaaacatt tacacctttg accaggtgcg cctgcgtttg tcaacagccc
     2041 ggtcagagtc tgcccaggca aaatacaatt ttctgttccg tgccaggatc ctcgctttct
     2101 ataacgggga aagccttggg ggtgactaaa atgtagttgc ccttttgctg tctacagctg
     2161 tcgctgaaac cacaagaacg ggaagctttt tcttttaggc cgaggtttcc cttatctttt
     2221 ggtgtcagcg acaccttctg gcagcagaaa caggctccac ctcttatgat cttgagcaac
     2281 aatgctgaaa aatacaagag tcgttgcccc ataaaggacg ggaagctttt tcttttaggc
     2341 cgaggctctc cgtcctttat ggggcaacga ctacctgaag ttaaagcctc ggcttaacac
     2401 cagggctaca ccgttctttc ttcagcctcc aggacaaagg cacgcaagcc ttgtttgggg
     2461 gcggattcgt ctgctttctt cagatcctgt agtaattggt ccaccacatg gtccggaaca
     2521 aaagtgatca gggcggaatt cagagttggc caggcatggc tacccaggtg cggttctccg
     2581 tcacgagatc catggccccg tacttcttcc cagaaggtaa aacctttaac atggttcttt
     2641 tccaaggctt tcatgacctc gtcataaaat gcctggtaga aagatataaa taatgctttc
     2701 atagttgttg tgctttacgc attttgcgtt tctcctttct ttctgccgtc aggctgtata
     2761 tggccggaat cagaaagagc gttacagcgg tggatacggt aagaccgctg gctacggtga
     2821 tacccaatcc gttccacatt tctgcaccca ctccgctgcc aacagccaaa ggcaccatcc
     2881 cgaatatggt ggtcaaagca gtcatcagga tgggacgcag acgcgagcgg ccggaggtta
     2941 caacggcttc ccggatggac atgccccgtt cccggcacag aacgatgtag tctatcaaaa
     3001 caatcccgtt tttcaccacc attccgaaga gcatcaacaa tcccaccatg ctcatgatac
     3061 ccagggcggt attgctgatg gcaagacccg ccagcactcc ggtaaatgca aagggtaccg
     3121 agaacatgat gataaaagga ctggagaatg attcaaactg agccgccatg acaatgtata
     3181 ccagtatgag gatcaaaatc atcatcatgc ccaagtcttt aaatgattcc tgctggtctt
     3241 cgtaactccc tgctatgctt accattactt ccggaggaat gtccactttt tctatctgct
     3301 gccgggcagc ggctacaagg tcactcagga cagctccttt tgcggcaacg caggttaccg
     3361 ttacaatccg ctccctgtct ttgcgttcaa tggtaggagg ttgcatggtc tcgccaatgg
     3421 cagccacatc ccgtaacctg attttttgcc cctggttgtt gtaaatcaga atattctgta
     3481 tgtcttc
//
LOCUS       DAOSOF010000168         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_27119_length_3480_cov_4.670949, whole genome shotgun sequence.
ACCESSION   DAOSOF010000168 DAOSOF010000000
VERSION     DAOSOF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27119_length_3480_cov_4.670949"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PLM68_08120"
     CDS             complement(<1..1335)
                     /locus_tag="PLM68_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT55358.1"
                     /translation="MDELKKISRRKALKYFGASVLGVAGVYAAVRYTPLKATLRESLK
                     KGDIRPITTRKDRVYGESISLLAYGCMRLPTMRNQEGNTVIDRDKSRELLKHAYENGI
                     NYFDTAYNYHNGESEIVTGEILKDFPRDSFCLATKMPTWLVTSLEKGKELFSEQLNKC
                     QVEYFDFYLLHALSSIDEYNKAYRDTGVLDYLKEEKTAGRIRRLGFSFHGNEETMDYL
                     LEQHNWDFVMIQMNYLDWEVQNAKYMYEQIEKKGIQCLVMEPLRGGALATLNKEAAGI
                     LAEADPDSNPARWAFRYVASHPNVLTILSGMNVMAHLEENINTFRKFKPLSKAEYEVL
                     ARALEAYRKISYVPCTACAYCMPCPYGVDIPLVFRTYNQMVVEHMVPDINGPIDEEFA
                     AKGQAFLSHFNDSIPAQNQPHNCTACGACKQHCPQEIDIPVEIKRINEIIWYI"
     gene            1401..1958
                     /locus_tag="PLM68_08125"
     CDS             1401..1958
                     /locus_tag="PLM68_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT55359.1"
                     /translation="MIKQKICAFIATRIMGWKLIGKPIPVPKCIILGVPHTSMWDFVI
                     SFLFYTGIGGNAKILVKSDVFVWPLRGLLRWAGAIPVDRGKGASFTRQTIEAIRNQET
                     IHLAIAPEGTRKATKKWKAGFHTLSRALDIPVYYGYFDWGRKEVGVAEQILITDDVQA
                     DMRRIKQWYIDKGVIGKHPERFDPY"
     gene            complement(1921..>3480)
                     /locus_tag="PLM68_08130"
     CDS             complement(1921..>3480)
                     /locus_tag="PLM68_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT55360.1"
                     /translation="KERYEQHHKVQIKDDALISAVELSHRYITNRFLPDKAIDLMDEA
                     AARLRLEMSSVPQCIDELDRKIRQLEIEREAIKRENDKEKIETLSREIEELSARCDVQ
                     RARWQEEKAVLEEIQQKKKQMDELRFAAAEAERRGDYGRVAEIRYGKIVELEKAIDEL
                     NKQKGSFTQSLIKESVTEEDIAEVVSKWTGIPVQRMLTAEREKLLHLEEELHKRVIGQ
                     DEAITAVSDAVRRSRAGLQDPRKPIGSFLFLGTTGVGKTELAKALAEFLFDDENLITR
                     IDMSEYQERHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFNI
                     LLQVLDDGRLTDNKGRVADFKNTILIMTSNMGSHLIQDSLQEDPDDAMAFEKVKRQVM
                     ELLRQTIRPEFLNRIDEIIMFHALTPENVRDILRLQLKIIADILLAKGIGIEFTDSAL
                     DYLQVKGYEPSYGARPLKRLLQKELVNELAIKILDGSLDGNLTVLVDSDGNGLNLRNL
                     NRDQTVQGASR"
BASE COUNT          789 a          919 c          787 g          985 t
ORIGIN      
        1 gatgtaccag atgatctcgt tgatcctttt tatctccacg ggtatgtcaa tttcctgggg
       61 acaatgttgt ttgcatgcac cgcaagccgt acagttgtgt ggctggttct gggcaggaat
      121 ggaatcattg aaatgtgaca gaaaagcctg tcctttggca gcaaattctt catctatagg
      181 gccattgata tcggggacca tatgctctac caccatctga ttataggttc tgaataccag
      241 cgggatatca actccatagg ggcagggcat acaataggca caggccgtgc acgggacata
      301 ggatatttta cggtatgctt ccagtgccct ggccaacact tcatattctg ccttgctcaa
      361 aggtttgaat ttcctgaaag tattgatgtt ttcctccagg tgggccatga cattcatccc
      421 gctcaaaatg gtcaatacgt taggatgtga tgccacatac ctgaatgccc acctggcagg
      481 attgctgtca ggatcagcct ctgccaggat gccggctgcc tctttgttca gggttgccag
      541 tgcaccgccg cgcaagggtt ccatcaccag acactggatg ccttttttct ctatctgttc
      601 atacatgtat ttggcattct gcacttccca gtccaggtaa ttcatctgga tcatcacaaa
      661 atcccaattg tgttgttcca gcaaataatc catggtttcc tcgtttccgt gaaatgaaaa
      721 accaagccgc ctgatgcgcc cggcagtctt ttcttccttc aggtaatcca gtactcccgt
      781 gtcgcggtat gccttgttgt actcatctat ggatgaaagg gcgtgcagca ggtaaaaatc
      841 aaagtattcc acctgacatt tattcaattg ctcactgaaa agctctttgc ctttttccag
      901 acttgttacc agccaggtag gcatcttggt agccaggcag aatgaatcac gcgggaaatc
      961 cttcagtatc tctcccgtga caatctcgga ttcgccgtta tggtaattgt aagcagtatc
     1021 aaagtaattg atcccgtttt cataggcatg tttcagcaat tcacgggact tatccctgtc
     1081 aatgaccgtg ttcccttcct ggtttcgcat ggtcggcaga cgcatgcacc catatgccag
     1141 gagggatatg ctttcaccat aaactctgtc ttttctggta gtgatgggcc ttatgtcgcc
     1201 ttttttcaat gactccctga gtgtggcttt caagggagtg taacgtacag cggcatagac
     1261 accggcaacc cccaggacgc tggctccaaa atacttcagg gcttttctcc tgcttatctt
     1321 ttttagttca tccacagtag tgatattatt tgaattcgtt agagatgcca aaattactca
     1381 ttttttatac ctttgttgca atgatcaagc aaaaaatctg cgcattcatt gccacacgaa
     1441 tcatgggatg gaaacttata gggaaaccca ttcccgtgcc caaatgcata atcctgggcg
     1501 taccccatac ttccatgtgg gattttgtga tttctttcct gttttatacc ggaatcggag
     1561 ggaacgccaa aatactggtc aagagcgacg ttttcgtatg gccgctcagg ggtctgttgc
     1621 gctgggccgg agccattccg gtagaccgtg gaaaaggcgc ctcttttacc aggcaaacga
     1681 tagaagctat ccgtaaccag gaaaccatcc accttgccat agcccccgaa ggcacccgca
     1741 aagccacaaa aaaatggaaa gcaggttttc acacactttc cagggcactt gacattcccg
     1801 tgtattacgg gtattttgac tggggacgca aagaagtcgg ggtggctgaa cagatactca
     1861 taaccgatga tgttcaagcc gatatgcgcc gtatcaaaca atggtatatt gacaaaggcg
     1921 ttatcgggaa gcaccctgaa cggtttgatc cctattaagg ttccgcaggt tcaatccatt
     1981 cccgtcgcta tccaccagaa cagtcaggtt tccatccaaa gacccgtcca gtattttgat
     2041 tgccagctcg ttaacaagct ccttctgtaa aaggcgcttt aacggtcttg ctccgtaaga
     2101 cggttcgtac cctttgacct gcaggtaatc gagtgcgcta tcagtgaatt cgatgcctat
     2161 gccttttgcc agcaggatgt ctgcaatgat tttcagttgc aacctgagga tatcccgtac
     2221 attttccggt gtcagggcat ggaacatgat gatctcgtct atacggttca ggaattccgg
     2281 ccgtatggtt tgacgaagca attccatcac ctgtcgcttt actttttcaa aagccatggc
     2341 atcgtcaggg tcttcctgca ggctgtcctg gatcagatga gaccccatat tggaggtcat
     2401 gatcaggatg gtattcttaa agtcggccac acgtccctta ttgtccgtaa gcctgccgtc
     2461 atccaggacc tgaagcagta tgttgaacac atcggggtgg gccttttcaa tttcgtccag
     2521 caacactacc gaatacggtt tcctgcggac agcttccgta agctgccccc cctcgtcgta
     2581 tcctacatat cccggaggcg cacccaccag gcggctgaca gaatggcgtt cctggtattc
     2641 actcatgtca atcctggtga tcaggttctc gtcgtcaaag agaaattctg ccagtgcttt
     2701 ggccagctcc gtctttccga cccccgtcgt gccaagaaac aggaaagaac cgatgggctt
     2761 gcgcggatcc tgcagacccg ccctgctgcg acgcacggca tctgacacgg ctgtaatggc
     2821 ttcatcctgc ccgataacac gcttgtgcaa ctcttcttcc aggtgaagca gtttctcacg
     2881 ttctgccgtg agcatccgct ggacaggtat ccctgtccat ttggaaacca cctcggcaat
     2941 gtcttcttct gtaaccgact ctttgataag ggactgggtg aaagaacctt tttgcttgtt
     3001 gagttcgtca atggctttct caagttccac aatctttcca tagcgtatct ctgccacccg
     3061 tccgtaatca ccgcgtcgct ccgcttctgc agctgcaaag cgcagctcat ccatctgctt
     3121 cttcttttgc tggatttcct ctaaaaccgc tttttcttcc tgccacctgg ccctttggac
     3181 atcacagcgg gccgataatt cctcaatttc cctgctaagt gtttcaatct tctccttatc
     3241 gttttcgcgt tttatggctt ccctttcaat ctccagctgg cgtatcttcc tatccagctc
     3301 atcaatgcat tggggtacag agctcatttc aagccttaac ctggccgccg cttcatccat
     3361 caggtcaatg gccttgtcgg gcaggaaccg gttggtgatg taacggtgag ataattccac
     3421 ggcagatatc agggcatcgt ccttgatctg caccttatgg tgctgttcgt atctttcttt
//
LOCUS       DAOSOF010000169         3469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_27233_length_3469_cov_5.441711, whole genome shotgun sequence.
ACCESSION   DAOSOF010000169 DAOSOF010000000
VERSION     DAOSOF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27233_length_3469_cov_5.441711"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..961)
                     /locus_tag="PLM68_08135"
     CDS             complement(26..961)
                     /locus_tag="PLM68_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOT55361.1"
                     /translation="MESTRQRIVRSVRAAAPGALKTAWWLVKIMFLVSFLITLLKYLG
                     VISWLSGLLTPFFSNFGLPGEAALAYVSGYFVNCYSAIAVMTSLNLDARAITILAVMV
                     LCAHNMPMETAVQRKTGTSAIRVVFVRTLCSLFLGWLLNKVMPGENAAYVAPPAIKEQ
                     ITFWPLMGDWILDTLKIAIPMVLLVFLLTIIQRLLSEFGVIRYIAKFLRPVMLFFGLP
                     AKTAFLWIVANTLGLAYGAAVMIDEVKEGKISVRDVDLLNHHICVSHSNVEDLLLFTA
                     IGGTLPWMLLSRWAMSLFLVWERKLERLIKKVGAK"
     gene            1027..2304
                     /locus_tag="PLM68_08140"
     CDS             1027..2304
                     /locus_tag="PLM68_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55362.1"
                     /translation="MNRFILFTLLLCLFSADSCKRTPKETAVALSSFKSLESVLQKDG
                     GALWGSAMYAPTFFVHPETKVIYADRKDEQGLLQPLDGIWTGTLPEQMNPANSTAELG
                     GMKWVMAIWPLPESEFERNVLLVHETFHYWQPELGLDSRLGLCEHMENTSARIWLKME
                     WNALDAAIREEDPESAAQAVGQALHFRNNRLSEFPAEARLNEDAFIILEGLPEYTSYK
                     LCCDDAVSMARQILESRKRHWNNESFVYAFAYHSGLAYGYLLDRYDVSWRSELNKDSS
                     LPDMLEKALPTFGEESPVNDSIILRQYGYDTIVASENEKEQEKIRLRDAYIHAFTRDT
                     VLAIPLRQFNMGFTPVNVQSLGQLGTLYPNIRITGDFGILEVTEGGCLISTDWSAAFV
                     TYKAPGWKLELKDGFDILPPAEATPLIYRIAEM"
     gene            complement(2283..3128)
                     /locus_tag="PLM68_08145"
     CDS             complement(2283..3128)
                     /locus_tag="PLM68_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT55363.1"
                     /translation="MKKIPALLILNVLYFLLPLTSAARGKYVVDSMESAILGEMRHFA
                     VYLPESYDNDTVREYPVLYLLHGGGGSWGDWGRVGNMVRIADQLMAAGESRQMIIVMP
                     DAGTTLMTYFNGGSWNFEDHFFNEFMPVVENRYRIRAEKGSRAIAGLSMGGQGSVMYG
                     CHRPDLFSVVYAMSAYLYRMEIAMFREDNPVNRDIQQRVEDHNAVKYIEGLSLDEVSP
                     LRSVTWVIDCGDDDFTYAANVEFSLAMKEKGIPLQVRIFDGAHSWEYWQAAFRRALPY
                     ISAIL"
     regulatory      3179..3390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          935 a          874 c          811 g          849 t
ORIGIN      
        1 gggtgcaagg ggtcgaatgt tcgagtcatt tcgccccgac tttttttatc agccgctcaa
       61 gttttctttc ccacacaaga aacagcgaca tggcccagcg cgagagcaac atccatggca
      121 gcgttccacc aatagccgta aacaggagaa gatcctccac attgctgtgg gaaacacata
      181 tatggtggtt cagcaggtcc acatctctga ccgatatttt tccctccttc acttcatcta
      241 tcataacagc agccccgtag gcaagtccca aagtattggc cacaatccat aaaaacgctg
      301 ttttggccgg caatccgaaa aacaacatca ccggtctcag gaatttggca atataacgta
      361 ttactccaaa ttccgacaac aaccgttgta tgatggtcag caggaatacg agcaacacca
      421 taggtatcgc tattttaagc gtatccagta tccagtcccc cattaatggc cagaaggtta
      481 tctgttcctt aatggccggc ggggcaacat atgcggcatt ttctccgggc atgaccttgt
      541 ttaatagcca ccccagaaac agagaacata aagtccttac aaaaaccaca cgtattgccg
      601 aggttccggt cttgcgctga actgctgttt ccatgggcat gttgtgagca caaagtacca
      661 tgaccgccag gattgttatg gccctggcgt ccaggttgag tgaggtcatt acggcaatgg
      721 ccgagtagca gttcacaaaa taacccgaca cataagccag tgcagcttca ccaggcaatc
      781 cgaaattcga gaaaaagggt gtaaggagtc cggacaacca tgaaatcaca cccagatact
      841 tcaacagggt gattaaaaat gaaaccagaa acataatctt caccagccac catgcagtct
      901 tcaatgcacc cggggcggca gccctgacag aacggacgat tctttggcgg gttgattcca
      961 tgttttgaat ttgaggcgca aatatactat tattttatag ttttgtgatt attaacaagt
     1021 cagcgtatga atcgttttat actatttact ttattactat gcctgttctc tgcggattcc
     1081 tgcaagagaa cgcctaaaga aacagctgta gcactttctt ctttcaagtc gctggagtcc
     1141 gttttacaaa aggacggcgg agctctttgg ggatctgcta tgtatgcgcc tactttcttt
     1201 gtgcatccgg aaactaaagt catctatgcc gacagaaagg acgaacaggg gctgctgcaa
     1261 ccacttgacg gaatatggac cggtacattg ccggaacaaa tgaatccggc caattcaact
     1321 gcagaactgg gcggcatgaa atgggtgatg gcaatctggc ctctgccgga aagtgaattt
     1381 gaaaggaacg tattgctggt ccatgaaaca tttcattact ggcaaccgga attggggctg
     1441 gattccaggc tggggctttg tgaacacatg gagaacactt ctgccaggat atggcttaag
     1501 atggaatgga acgccctgga cgcggccatc agggaagaag accccgaatc tgctgctcaa
     1561 gctgttggcc aggctttgca tttcaggaac aaccgcctgt cggaattccc tgcagaagca
     1621 cggctcaatg aagacgcttt tatcatcctg gaaggtcttc ccgaatatac ctcctacaag
     1681 ctttgctgcg atgatgcagt tagtatggcg cgacaaatcc tggagagccg caagcgtcac
     1741 tggaacaacg aatcgttcgt atatgccttt gcctaccatt cgggactggc atacggctat
     1801 ttactggacc gttatgatgt ttcctggcgg agtgagctga ataaagactc ctccctgccc
     1861 gacatgttgg aaaaagcact gcctaccttt ggggaagaat ccccggtcaa tgattctatt
     1921 atcctgagac aatacggata cgacaccata gtcgcgtcag aaaacgaaaa ggagcaggaa
     1981 aaaatacgtt tacgggatgc ctatatccat gcttttaccc gggataccgt cctggccatt
     2041 cccctaaggc aattcaacat gggattcacc cctgtcaatg tgcagtcact tggccagctg
     2101 gggacattgt accccaatat ccggattacc ggggattttg gaatattgga ggtaactgaa
     2161 ggcgggtgtc tgatctctac agactggtct gcggctttcg tcacttacaa ggctcccgga
     2221 tggaaactgg agcttaagga tggattcgac atcctcccgc ctgctgaagc aactcctctt
     2281 atttacagga ttgccgagat gtagggaagt gccctgcgaa aggctgcctg ccagtattcc
     2341 caggaatgtg ccccgtcgaa tatccttacc tgcaaaggaa tgcccttctc tttcatggca
     2401 agggagaatt ccacgttggc ggcataagtg aagtcgtcgt caccacaatc gatgacccag
     2461 gtcacagagc gtaacgggga cacctcatcc agggaaagcc cttcaatgta tttcacggca
     2521 ttatgatcct ctaccctttg ctggatatcc ctgttcacag gattatcttc cctgaacatg
     2581 gcaatttcca ttctgtacag gtaggcgctc atggcataaa caaccgagaa aaggtccggc
     2641 ctgtggcatc cgtacatgac tgaaccctga cctcccatgg acaagccggc aatggcacgt
     2701 gatccttttt ctgcacgtat cctgtaacgg ttttccacca cgggcatgaa ttcattgaaa
     2761 aagtggtcct caaaattcca gcttcccccg ttgaaataag tcataagggt cgtacccgcg
     2821 tccggcatga caatgatcat ctgccgggac tccccggcgg ccatcaattg gtccgctatt
     2881 cggaccatgt tcccaacgcg gccccagtca ccccaggaac ctcctcctcc atgcaacaaa
     2941 taaaggaccg gatactccct gacggtatca ttgtcatagc tttcgggaag atatactgca
     3001 aagtgacgca tttctcccag gatggcactt tccatggagt cgaccacgta tttgcccctg
     3061 gccgctgaag tcaagggaag taaaaaatac aagacattca gaatcaataa agcagggatt
     3121 tttttcacgg cattttttta tttcttcaca aaattagcac aatattgtcc aaaacgacct
     3181 atatttgcat tgcaacggtt ctgaaggacc tgaaaacggc ccggaagatt aaaagggaat
     3241 gccgtgaaaa tcggcaacag ttcccgctgc tgtgaatccc aggaacggaa gttccaaagg
     3301 atctgtcaat cttgccactg acctaacggt tcgggaaggc gccagataaa gggataagtc
     3361 agaagacctg ccgttgtact attagcggag aactaactaa ttttccagaa catgaaacat
     3421 ttgtcttttt tatgtgcatt gtgcctgaca ggacttttgt caggctgcc
//
LOCUS       DAOSOF010000170         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_27371_length_3456_cov_4.955013, whole genome shotgun sequence.
ACCESSION   DAOSOF010000170 DAOSOF010000000
VERSION     DAOSOF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27371_length_3456_cov_4.955013"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="PLM68_08150"
     CDS             complement(<1..1554)
                     /locus_tag="PLM68_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT55364.1"
                     /translation="MKPFKKLFPLFFSVLLACSCASSANTDLNIEKKVAGLLSNMTLD
                     EKIGQMTLISSTDLPLDGEFAEALRKGQVGSVINEVDPARVNALQRMAVEESRLGIPL
                     IVGRDVIHGFKTIFPIPLGLAATFNPTLVEQGARVAALEATAAGVRWTFSPMLDITRD
                     PRWGRIAEGFGEDTYFTSAMGVAMIRGYQGGEPGDPSNMAACIKHFVGYGAAEGGRDY
                     NSTNLPERVLRNVYLPPFLAGVQAGAMTLMTSFNDNDGVPMTGNRFLVRNVLRHEWDF
                     TGLVVSDWASVNEMIPHGFATDQAHAARLALEAGVDMDMMSFAYVGHLGKLVGEGAVS
                     EKMIDEAVRNILRVKFQLGLFDNPYVQEDVHREMFYKPEHLELSRQSAVQSVILLSND
                     GTLPLDADKIRSVLVTGPMADAPHDQLGTWVFDGEEQATVTPLKAIREHYGHRLRVLY
                     QPGLKYSRDKDLSGIRKAVEAARTVDVVIVCVGEESILSGEAHCLASLDLQGTQSELI
                     AALKSTGKPV"
     gene            complement(1565..2665)
                     /locus_tag="PLM68_08155"
     CDS             complement(1565..2665)
                     /locus_tag="PLM68_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOT55365.1"
                     /translation="MKKLFFIIASITLTTCGCNGGSGPEPNGQPQITLSEEKLEFGFS
                     AGAKTITVTADREWGVAADQDWCTTFPTGGLEGQTILTVNTTENRTGKDRTCILSFRS
                     GDYKKELTLLQHLNPADTTSIIDPGVEVPAGYKLIWRDEFDKLDNAEWWFETGAGGWG
                     NNELQRYIPGVEGKDTCARVSGGTLEIMAKQSGNEVLSIRMNTQRSWTYGYFEARLKL
                     PVGKGTWPAFWMMPKNFKAWPDDGEIDIMEHVGYRPNWISSTIHCKSYNHASGTQKTA
                     EKFIATAQSEFHTYALEWTENAIYGYVDGIRYFTFTNDQKGIYDSWPFYKPFYLKLNL
                     AWGGNWGGAEGVDPAALPAIFEIDYVRVFQKK"
     gene            complement(2674..>3456)
                     /locus_tag="PLM68_08160"
     CDS             complement(2674..>3456)
                     /locus_tag="PLM68_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55366.1"
                     /translation="VNKDSGLRPDLNPGDGNDYAIPYETKSTTWNLYTEGGVMYLEFP
                     AETVVGYVPNPQGYSTPRFRVLNLTTDLLELALDGPGITWHYLFVPVSFEGDETSASS
                     FASGLPGSWTWEPSVLGHFGCGPSGGNGLDWWSAAPYDKQGQGLYDDILTFKKDGSYV
                     FDPGPGGTIYCNWECGFHPEYGTGEQNVDYAVPVQVQTSTYVLEEGVIRWPSNTFVSY
                     IPNAQTLANPVYKITKMTTYILELVTDNGGIAWHYRFQRYAK"
BASE COUNT          765 a          957 c          855 g          879 t
ORIGIN      
        1 gacaggtttt cctgtagatt tcaacgccgc tatcagttcg ctttgtgtcc cctgcaggtc
       61 cagactggcc aggcagtgtg cctcccccga gaggatggat tcttctccca cgcatacaat
      121 cacaacatcg acagtgcgtg ccgcttcaac ggctttcctt attcccgaca ggtctttgtc
      181 ccttgaatat ttcaatcccg gctgataaag aacgcgcaac cggtgaccgt aatgctctct
      241 tatagctttc aaaggagtga cagttgcctg ttcctctcca tcaaaaaccc aggttcccag
      301 ctggtcatgc ggcgcgtcgg ccatggggcc tgtgacaagt acagacctga tcttgtctgc
      361 atccagggga agggtaccgt cgttgctcaa cagaatgaca ctttgaacag cgctttgcct
      421 ggaaagttcc aggtgctcgg gtttatagaa catttccctg tgcacatctt cctgcacata
      481 gggattgtcg aacaatccca gctggaattt aacgcgcagg atgtttctta cggcctcatc
      541 aatcattttt tcagacacag ccccttcccc gaccagtttc cccagatgcc cgacgtaagc
      601 aaaggacatc atgtccatat ccacgccggc ctccagggcc agtctggccg catgggcctg
      661 atccgtggca aacccgtggg gtatcatttc attgacagag gcccagtcgc tgactaccaa
      721 ccccgtgaaa tcccattcat ggcgcaagac attccttacc aggaaacggt tcccggtcat
      781 gggaacccca tcgttgtcgt tgaaagaggt cataagtgtc atcgcacccg cctgaacccc
      841 ggccaggaaa ggcggcaggt acacattgcg taatacccgt tctgggaggt tagtgctgtt
      901 ataatcccgg cctccttcag ccgctccgta acccacaaaa tgcttgatac aggctgccat
      961 gttcgaagga tcaccaggtt caccgccttg ataacccctt atcatggcca ctcccatggc
     1021 cgaggtgaag tatgtgtctt ctccgaaacc ttctgcaatc cttccccagc gtggatccct
     1081 ggtaatgtcg agcatgggtg agaaagtcca gcgcactcct gcagcagtgg cttccagggc
     1141 ggctacacgc gcaccttgtt ccaccagtgt ggggttgaat gtggctgcca gtcccagggg
     1201 tatcgggaat atggtcttga atccgtggat cacatcccgg cccactatca ggggtatacc
     1261 caggcgggat tcttcaacag ccattctttg cagggcattc accctggcag gatccacctc
     1321 gttgataacg gatcccacct gtcctttgcg caatgcctcg gcaaactctc cgtccagcgg
     1381 taagtcggta gaggagatca atgtcatctg acctattttc tcatcaaggg tcatattgga
     1441 taacaaaccg gcgacttttt tttcaatatt caggtctgta tttgctgaag acgcacagga
     1501 gcatgccagc agaacagaga aaaacagcgg gaataacttt ttaaacggtt tcatagaaac
     1561 gggattattt tttctgaaaa acgcgtacgt aatcaatttc aaaaatggcc ggcagggctg
     1621 ctggatcaac gccttctgcg ccaccccagt ttccacccca ggccaggttc agtttcagat
     1681 aaaagggctt gtagaaaggc cagctgtcat agattccttt ctggtcattg gtgaaagtaa
     1741 aataacggat gccgtctaca tacccgtata tggcattttc tgtccattcc agcgcatatg
     1801 tgtggaattc actctgggcc gtggcgatga atttttcggc agttttctgg gtacctgaag
     1861 cgtggttgta gctcttgcaa tgtatggtgc tgctgatcca gttggggcgg tatcctacgt
     1921 gctccattat gtcaatttcg ccgtcatcgg gccatgcctt gaaattcttg ggcatcatcc
     1981 agaaggccgg ccaggttcct ttgcccacag ggagcttcag gcgcgcctcg aaataaccgt
     2041 atgtccagct gcgctgcgta ttcattcgga tggaaagtac ttcattaccc gattgttttg
     2101 ccatgatttc cagcgtcccg ccggagaccc tggcacatgt gtcttttccc tcaacccccg
     2161 ggatgtaccg ctggagttcg ttgtttcccc agcctcctgc cccggtttca aaccaccatt
     2221 ccgcattatc cagcttgtca aactcatcgc gccatatcag tttataccct gcaggcacct
     2281 caaccccggg atcaataatg gaggttgtat ctgcaggatt gaggtgttgc aacaaggtaa
     2341 gctctttttt ataatcgcct gaacgaaagg ataaaataca tgttctgtcc ttcccggtcc
     2401 tgttctccgt cgtgttcaca gtcaagattg tctgaccttc cagacctcct gtaggaaagg
     2461 tggtacacca atcctggtct gcggcaacac cccattccct gtcagctgtg acagtgatgg
     2521 tctttgcccc ggctgaaaat ccgaattcca gcttttcttc agaaagggtt atttgcggtt
     2581 ggccgtttgg ttcgggcccg ctgccgccgt tacacccaca ggtggtcagg gttatggagg
     2641 caatgataaa aaagagcttt ttcatgcttg tctttatttt gcgtatctct ggaaacggta
     2701 atgccaggca atgcctccgt tgtctgtgac cagctcaaga atgtacgtgg tcatcttcgt
     2761 gatcttgtag acagggtttg ccaatgtctg tgcattgggg atgtagctga caaatgtatt
     2821 tgacggccac ctgatcacac cttcttccag aacataggtg gatgtctgca cctgcacagg
     2881 aacggcataa tccacgtttt gctcgccggt gccgtattca ggatggaatc cgcattccca
     2941 gttgcaataa atggtgccgc cgggacccgg atcaaataca tatgatccgt ctttcttgaa
     3001 ggtgaggata tcgtcataca aaccctgtcc ctgtttgtcg tagggagcag cactccacca
     3061 gtccagaccg ttgcccccac tggggccgca tccaaagtgt cccagcacag agggttccca
     3121 tgtccaggac cctgggagtc cgcttgcaaa actggaagcg ctggtctcgt ccccttcaaa
     3181 cgacacggga acaaaaaggt agtgccatgt gatgccagga ccgtcgagcg ccagctccag
     3241 caggtcggtt gtcaggttga gcacgcggaa acgcggtgtg gaataacctt gaggattcgg
     3301 aacgtatccc accaccgttt cggccgggaa ctccaggtac atcacaccgc cttccgtata
     3361 caggttccag gtggtggact tcgtttcata aggaatggca taatcattcc cgtcgcccgg
     3421 gttcaggtcg ggacgcagtc cgctgtcctt gttgac
//
LOCUS       DAOSOF010000171         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_28605_length_3341_cov_3.167377, whole genome shotgun sequence.
ACCESSION   DAOSOF010000171 DAOSOF010000000
VERSION     DAOSOF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28605_length_3341_cov_3.167377"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLM68_08165"
     CDS             complement(<1..1037)
                     /locus_tag="PLM68_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT55367.1"
                     /translation="MKRFFFLIVLLTFMTTTASAVQTGKPVVQRIEPPCWWVGMETQL
                     QLMIHGPGLGEGTVTVLTPGIAIAAVHKADSPNYLFLDINIPAGVPAGDYTFQWSNGK
                     KKLNFVYTLHERTENSSKRFGLSSSDVLYLLMPDRFVNGDLENDSTPCTAEKADPKDD
                     YGRQGGDLEGIRMSLDYLEELGITSVWPTPVTLDDEPAVSYHGYACADYYKIDPRFGT
                     NETYRELVKEAASRGISFLQDIVPNHCGTAHWWMKDLPFKDWIHVFGTYTRSNYAMST
                     HMDPHAAAIDNELCTQGWFDTSMPDMNLENPFVLQYFIQATVWWMEYAGLAGVRIDTY
                     PYSNEKAISAY"
     gene            complement(1042..2382)
                     /locus_tag="PLM68_08170"
     CDS             complement(1042..2382)
                     /locus_tag="PLM68_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT55368.1"
                     /translation="MKKSLIIISFMMMLAGGCTQQGGEEIHPQWIRNAVIYEVNTRQI
                     TTEGTFNAFADMLPGLKELGVDVLWFMPIYPIGMEGRKGTLGSYYSIRDYGAVNPEFG
                     TLEDFKLLVDKAHELGMKVILDWVAAHTSRDAVWLDQQDWYIRRPDGEPEFLYDWSDV
                     ARLNYNNQEMRQAMLDKMLFWVEEVNVDGFRCDMADLTPVDFWNWAVPVLKEHKPGIF
                     MLAESENPINTREAFNAYYAWRFHHTMNSVARGEKVADSLRKDLNSMNTEFGPHAIPL
                     LFTSNHDENSWSGTEFERMGDAALQMAAFTFVLPGIPLIYTGQEVGNIKRLQFFEKDT
                     LQPSDPDSFMEFYKNLIKLKKSSDALQVPPYGGILQEVPHTAASSVFAFIREKAGSKV
                     LAIFNFDSQPVSFRFTGNLADGTYTDWNDQTLFNVTPDYNWDMEPYGFRILTSQ"
     gene            complement(2392..>3341)
                     /locus_tag="PLM68_08175"
     CDS             complement(2392..>3341)
                     /locus_tag="PLM68_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT55369.1"
                     /translation="TWFDKHIPKFDFSRPDVCQALSDSALFWLQHFDLDGFRHDATKH
                     IDELFWRTLTRKITKEFPDRTVLQIGETYGSPSLIGSYVKKGMLDGQFDFNLYYAFLN
                     ATVAPGGSFENLWDQLETCLIQYGYHNLMGNISGNHDMARYISLAGGDLGLDENQKLA
                     GWKRRIGTGKPESFDYLAMLHALNMTLPGIPCIYYGDEIGMPGANDPDNRRMMIFEGY
                     TPQQVHLKERVRRLTSLRSTNLALLYGDLFPLHVSRDVLAYVRIYMGKAVAVYLNKGH
                     DFFDIRSLSLPFGLSTDSMVPYMGETVVAPQGFTIMVIE"
BASE COUNT          831 a          834 c          769 g          907 t
ORIGIN      
        1 ttataggcgg aaatggcttt ctcgtttgaa tagggatacg tatcaatcct gactcccgca
       61 agaccggcgt attccatcca ccagaccgtg gcctggataa aatactgcag tacaaatgga
      121 ttctccaggt tcatatcggg catggaggta tcaaaccacc cctgtgtaca caattcattg
      181 tcaatggctg cagcatgggg gtccatatgg gtactcatgg catagttgga gcgtgtatat
      241 gtgccaaaga catggatcca gtctttaaaa ggcagatctt tcatccacca gtgggccgtg
      301 ccgcaatggt tgggcactat gtcctgcaga aatgatatgc cccttgatgc tgcctccttt
      361 acgagttccc tgtaagtttc attcgttccg aaacgcggat cgatcttata ataatcggca
      421 caggcgtatc cgtggtacga taccgccggc tcatcatcaa gggtaacggg tgtcggccag
      481 acagaagtaa tccccagctc ttcaaggtaa tccaggctca tgcgtatccc ttccaggtct
      541 cctccctgac gtccgtaatc atctttaggg tccgcttttt ctgccgtgca gggagtggaa
      601 tcattttcga gatctccgtt cacaaaccgg tcaggcatga gcaggtacag tacatccgag
      661 gaagaaagcc cgaaacgttt tgaggaattc tcagtccttt catgcagggt ataaacgaaa
      721 ttcaatttct tttttccgtt tgaccactgg aatgtataat ctcccgccgg aacacccgcg
      781 ggtatgttta tatccagaaa caagtaattg gggctgtctg ctttgtgaac agcagcaata
      841 gctataccgg gagtcaacac ggtgaccgtt ccttcgccca gacccgggcc atggatcatg
      901 agctgcagct gtgtttccat accaacccac cagcaaggag gttctatgcg ttgcactacc
      961 ggtttaccgg tttgtacagc gctggcagta gttgtcataa atgtaagaag gacgataagg
     1021 aaaaaaaatc ttttcatggc tttattgtga agtcaggatc ctgaaaccat acggttccat
     1081 atcccagttg taatcaggag taacgttgaa aagggtttga tcattccagt ccgtatatgt
     1141 cccgtctgcc aggtttcctg taaacctgaa agagaccggc tggctgtcaa aattgaaaat
     1201 ggccagcact ttgctgcctg ctttttccct tatgaaagca aatactgagg aggcagctgt
     1261 gtggggcact tcctggagga tgcctccgta tggaggtacc tgcaaagcat ctgaactctt
     1321 ttttaacttg atgaggtttt tatagaattc catgaaagaa tcgggatcgg aaggttgcag
     1381 ggtgtctttt tcaaagaatt gcagtctttt gatgttaccc acttcctgtc ccgtgtaaat
     1441 caggggaatt ccgggaagca caaaggtgaa ggctgccatc tgcagtgccg cgtctcccat
     1501 tcgttcaaac tcggtcccgc tccatgaatt ctcatcatgg tttgaggtaa agagcagggg
     1561 gatggcatgc ggaccaaatt cagtgttcat gctattcagg tcttttctca ggctgtccgc
     1621 aaccttttct cctctggcca cactgttcat ggtatggtga aatctccagg catagtatgc
     1681 attgaatgcc tccctggtat ttatgggatt ctctgattcc gccagcatga agataccggg
     1741 tttgtgctct ttcaagacag gaacggccca attccagaaa tcgacaggtg tcagatcggc
     1801 catatcgcag cggaaaccgt ccacattgac ctcttccacc cagaaaagca ttttatccag
     1861 catggcctgg cgcatctcct ggttattgta gttcaaacgt gccacatcgg accagtcata
     1921 caggaattcg ggctctccgt cgggccttct tatataccag tcttgctggt ccagccaaac
     1981 ggcatcgcgg gaagtgtgag ctgccaccca gtccagaatg accttcatac ccagttcgtg
     2041 tgctttgtca accaacagtt tgaaatcctc caatgttccg aattccggat tgaccgcgcc
     2101 gtaatccctt atggaataat atgagccgag ggttccttta cgtccttcca tcccgatagg
     2161 atagatgggc ataaaccata acacatccac ccccagttcc tttaatccgg gcaacatgtc
     2221 ggcaaaggcg ttgaaagttc cctccgtagt tatctgtctt gtgttcacct cataaataac
     2281 ggcatttcta atccattgtg gatggatttc ttctccgccc tgttgtgtgc atcctcctgc
     2341 taacatcatc ataaaactaa taataatcag tgactttttc attgtattct gtcattcaat
     2401 taccatgatt gtgaaacctt gcggtgctac aacggtttct cccatatacg ggaccatact
     2461 gtcggtgctc aggccaaaag gcaaactaag ggaacggata tcgaagaaat catgaccctt
     2521 gttcaggtac actgcaacgg cttttcccat gtaaatgcgc acataggcca gcacatccct
     2581 gctcacgtga agcgggaaca gatcaccata gagcagggca aggtttgtgc ttctgagcga
     2641 tgttaacctc cttacccttt ctttcagatg aacctgttgt ggtgtatagc cttcaaagat
     2701 catcatcctg cggttgtccg ggtcgttggc tcccggcatt ccaatttcat ctccgtaata
     2761 tatgcaggga attccaggca aggtcatgtt caaggcgtgt agcatggcca ggtaatcgaa
     2821 ggattcgggt ttaccggtac caatccttct tttccagcct gccagcttct ggttttcatc
     2881 caagcccaga tcgcctccgg caagggatat ataccttgcc atgtcgtggt tgccgctgat
     2941 attccccatc aggttatgat acccatattg aatcagacat gtttccagct gatcccataa
     3001 attctcaaaa gatcctcccg gggcaacggt ggcgttcaaa aaagcatagt acaggttgaa
     3061 atcaaactgg ccatccagca ttcctttttt tacgtaacta cctatcaaag aaggtgaacc
     3121 atacgtttct ccaatctgta gaactgtcct gtcgggaaac tccttggtta ttttccgggt
     3181 aagcgtcctc cagaacaact cgtcgatgtg tttggtggca tcatgccgaa aaccgtctaa
     3241 atcaaaatgt tgcaaccaga aaagcgcgga atcggacaat gcctggcata catccggcct
     3301 tgaaaaatca aatttgggaa tgtgtttgtc aaaccaggtg g
//
LOCUS       DAOSOF010000172         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29185_length_3290_cov_4.392272, whole genome shotgun sequence.
ACCESSION   DAOSOF010000172 DAOSOF010000000
VERSION     DAOSOF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29185_length_3290_cov_4.392272"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLM68_08180"
     CDS             complement(<1..290)
                     /locus_tag="PLM68_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HOT55370.1"
                     /translation="MLLLAFATSGCIGPEEHDAFVRVENGQFIRNGEPYRFIGFNFWY
                     AGLLASEGTGGDRERLHRELDYLKSVGIDNLRIMVGADGPENRAKVRPSLQT"
     gene            complement(319..2061)
                     /locus_tag="PLM68_08185"
     CDS             complement(319..2061)
                     /locus_tag="PLM68_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT55371.1"
                     /translation="MKKTLLSILTLMLILGCDQTPQVREAELDPSWKFQTGNDLQWAL
                     PGFDDSQWPEISPATSWENQGYANYDGYGWYRKTFTIPQDIIKAVIHYGGLSIRYDNA
                     DDSDALYFNGQIIGATGSFPPDYISRYGEKRTYTVPLEYIKTDEPNTIAIQVYDGGGD
                     GGLLTSAIIIQPVAPALNISMEYVVDAENGVFMGEVPLTLSVTVNNQEEKTKKSVIHL
                     AVTTDDYQPVQEFSRKITVRSQESMTYVLPLKISNPGFYRCTLRLETDGVKGEPLTFN
                     IGLEPEKIISPIDAQEDFDAFWQETRSILDSTPMRPSMKLLKEYSTGKRNIYYVEMKS
                     FGDEIMGGYYAVPKEKGKYPVIITYMGYGSNPWMPHTDGEPGFISFVVSVRGQGIYKA
                     GNKYNDKWIVWGIEDKNDYYYRGAFMDLVRAIDFVCSRPEADKDRIAAEGGSQGGAFT
                     MAACALDNRIAVAAPTIPFLSDYPDYFRIVDWPRSDIDAYIREHPQATWEDIYKLLSY
                     FDIKNLAPRIKTPTIMGVGLQDPTCPPHTNFAGYNLITAPKDYRIYQDNGHNTPPEWQ
                     EVRMEFFKKHLELD"
     gene            2178..>3290
                     /locus_tag="PLM68_08190"
     CDS             2178..>3290
                     /locus_tag="PLM68_08190"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOT55372.1"
                     /translation="MRRITLLSMTALIFAASCSGGKGDNPPYMDPSLDVEKRVTDLMK
                     RMTLEEKIGQMNQCVGLNHIDESQAGMKEEDLMRNTAQAFYPGYLKEDIAQLAREGKI
                     GSFLHSVNIGEANELQQLAMESRLRIPLLMGIDAVHGCALVSGTTVYPTSIGMASTFD
                     PELVYISSRQTAKEMRAIGMHWTFAPNVEVARDQRWGRVGETYGEDPYLVSLMGSAAV
                     KGFQGDEGLEKDLVLACAKHFVGGSQPVNGTNGASAELSERTLREVFFPPFRACVDAG
                     VETFMMAHNEIEGIPCHTNAWLMQDIIRKEMGFDGFIVSDWMDMEHVYDLHRTAVSVE
                     DAYAQSVLSGMDMHMHGPGFVPAMLKLVEDGVVPVSR"
BASE COUNT          748 a          789 c          881 g          872 t
ORIGIN      
        1 tcggtctgca ggctgggtct tactttggcc ctgttttccg ggccatctgc acctaccatg
       61 atacgcaggt tgtctatccc tacggatttc agataatcga gttcccggtg cagcctttcc
      121 cggtcacctc cggtaccttc cgaagccagc agaccggcat accaaaaatt gaatcctatg
      181 aaacggtagg gttctccgtt gcgaataaac tgcccgttct ccacacggac aaaggcatca
      241 tgctcttccg gcccgatgca accggaggtc gcgaatgcca gcaacagcat tccgcagaat
      301 aatatcattt ttttcatgtc agtcgagttc caggtgtttt ttgaaaaact ccatgcgcac
      361 ttcctgccac tcaggcgggg tattgtgccc gttgtcctgg tagatcctgt aatccttggg
      421 tgctgtgatc aggttgtagc cggcaaaatt ggtgtgaggg ggacacgtgg ggtcctgcag
      481 tccgactccc atgatagtgg gggttttgat gcgtggggcc aggtttttga tgtcaaaata
      541 gctcagaagc ttatagatgt cttcccaagt ggcctgcgga tgttctctta tgtaggcatc
      601 aatatcgctc cggggccagt ccacgatccg gaaataatcg ggataatcac tcaaaaaggg
      661 tatggttggt gcggcaacgg caatacggtt gtccagcgca caggcggcca tggtgaaggc
      721 acctccctgg ctgccgcctt ctgcggctat ccggtcttta tcggcttcgg gccttgaaca
      781 gacaaaatca atggcccgca ccaggtccat gaaggcgccc ctgtagtagt aatcgttttt
      841 atcctctata ccccatacga tccatttgtc gttgtatttg tttccggctt tatagattcc
      901 ctgtcccctc acagatacaa cgaatgagat gaatccgggt tccccgtcgg tatgcggcat
      961 ccagggattg gatccgtagc ccatataggt tatgatcacc gggtattttc ctttttcttt
     1021 gggaacagcg tagtatccgc ccatgatttc gtccccgaat gatttcattt ctacgtaata
     1081 aatgtttctt ttccccgtgg aatactcttt cagcagtttc atggatggcc gcatgggggt
     1141 gctgtcgaga atggatcgtg tctcttgcca gaaagcgtca aaatcttcct gggcatcgat
     1201 aggtgaaatg atcttttcgg gctccagacc gatattgaat gtaaggggtt ctcccttgac
     1261 accgtccgtt tcaagacgga gggtgcagcg gtagaatcca ggattggaaa ttttcagggg
     1321 aaggacatag gtcatgctct cttgtgacct tactgtaatt ttccgggaaa attcctgaac
     1381 cggctggtaa tcgtcagtgg taacggccag gtgtatgaca gactttttgg ttttttcttc
     1441 ctggttgtta actgttacgc taagggtaag tggtacctct cccatgaata cgccgttctc
     1501 tgcatctaca acgtattcca tggatatgtt cagggcaggg gcaacgggct ggatgattat
     1561 agcggaggtc agaagaccgc cgtcaccccc tccgtcgtat acctggatgg ctatggtgtt
     1621 gggttcatcg gttttgatgt attccagcgg aacagtgtac gtcctttttt ccccgtaacg
     1681 gcttatgtaa tcggggggaa aactgccggt tgccccaatt atctgcccgt tgaaatacag
     1741 ggcatctgaa tcgtctgcgt tgtcatagcg gatcgacagt cctccgtaat gaataaccgc
     1801 ctttatgatg tcttgcggga tggtgaatgt cttgcggtac catccgtatc cgtcgtaatt
     1861 ggcatagccc tggttttccc aggatgtggc aggggatatt tccggccact ggctgtcatc
     1921 aaaaccgggc aaagcccatt gcaggtcgtt cccggtctgg aatttccagg aagggtcaag
     1981 ttccgcttcc ctgacctggg gtgtctggtc gcaacccaat ataagcatga gggtcagtat
     2041 agacaacaat gtttttttca tatgatttta gttaaatgtt tgaccgaatg ttttgaaagt
     2101 gtaaatgtaa cacttttagg ctgataaaat attggctaca tttgttaccc gttgaattct
     2161 gtaaaataat ctgaactatg agaagaataa cattattatc tatgacagct ttgatttttg
     2221 ccgcttcctg ttcgggcggg aagggagata atccccccta tatggacccg tcactggacg
     2281 ttgaaaagag ggttacagac cttatgaaaa gaatgacatt ggaggaaaaa ataggccaga
     2341 tgaaccagtg tgtagggctc aaccatattg acgaatcaca ggccggcatg aaagaggaag
     2401 accttatgcg caatacggca caggcttttt atcccgggta cctgaaggaa gacattgccc
     2461 agcttgccag ggaaggaaag atagggtcat tcctgcattc tgtgaatatt ggcgaggcca
     2521 atgaactaca gcagctggcc atggaatcca ggctgcgtat tccattgctg atgggtatag
     2581 atgccgttca tggctgtgcc ctggtcagcg ggaccaccgt ttatcccact tccataggaa
     2641 tggcttccac ttttgatccg gagctggtgt acatttcttc ccgccagact gcaaaagaga
     2701 tgcgggccat aggaatgcac tggacattcg cacccaacgt ggaagttgcc cgggaccagc
     2761 ggtggggaag ggtaggcgaa acatacgggg aggaccctta cctggtatcc cttatgggga
     2821 gtgcagctgt aaaaggtttt cagggtgatg aaggcctgga aaaagacctg gtcctggcct
     2881 gcgccaagca ttttgttggc ggatcgcagc cggtaaacgg caccaacggc gcatccgccg
     2941 aactatccga aaggacattg cgggaagtct tctttccccc gttccgtgcc tgtgttgacg
     3001 ccggggtaga gaccttcatg atggcccaca acgaaatcga aggcattccc tgccatacca
     3061 atgcctggct catgcaggac atcatccgga aggagatggg ctttgacggt tttattgtca
     3121 gtgactggat ggacatggaa catgtatatg atctccaccg cacagctgtc tcggtggagg
     3181 atgcctacgc ccagtcggtg ctctcaggca tggacatgca catgcacggg cccggatttg
     3241 tgccggccat gctcaaactg gtggaggacg gtgtggttcc tgtgtcaagg
//
LOCUS       DAOSOF010000173         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29291_length_3280_cov_4.889302, whole genome shotgun sequence.
ACCESSION   DAOSOF010000173 DAOSOF010000000
VERSION     DAOSOF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29291_length_3280_cov_4.889302"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLM68_08195"
     CDS             complement(<1..187)
                     /locus_tag="PLM68_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55373.1"
                     /translation="MKKNNVRTILILLPFLLCGYHMHAGSSVADSTSSTAGRSSSVGN
                     STSSVADSTSSVADSTSS"
     gene            complement(174..1307)
                     /locus_tag="PLM68_08200"
     CDS             complement(174..1307)
                     /locus_tag="PLM68_08200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT55374.1"
                     /translation="MNPVSSTFKDTKKHYEILDALRGVAALVVVLFHLLETFTNGNNH
                     IQLINHGYLAVDFFFVLSGFVIGYAYDDRWGKMSLGNFFKRRLIRLHPMIIVGMFIGA
                     VTLFFQQAAIFPAIAQTPLWKVIVVMVLGFALIPVGPNLDLRGWNETFPLNGPAWTLF
                     FEYIANILYGLFIHKFSKKALAVLAVISGAALVHLAVTRGDIIGGWSLEPLQLRIGFT
                     RLLYPFFAGLLLSRVVKPGSVKNAFLWCSLLLVAILSVPRLGGTEHIWINGLYVSVSI
                     IILFPLIIYMGASGNVTGRFSTKLCRFLGDISYPVYIIHYPLIYMFMAWVFKNQVSMK
                     ASVPMALFIFFLTIALSYGIYRIYDVPVRKWLTRKITPNNEKK"
     gene            complement(1304..2206)
                     /locus_tag="PLM68_08205"
     CDS             complement(1304..2206)
                     /locus_tag="PLM68_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT55375.1"
                     /translation="MSKHAEIRRYSLVIEKIRSGHYPTFREIRDFLHQHGFQVGDRTI
                     QRDMEQIRFEFGVEIKFDRTRRGYCIDYEESVNIESFFHFLEIVNTAELLTQSLMESK
                     DALRHISFDTGGGLKGIHNLKPLLRAVRERRQVSFLHYNFQTDKTTDYTVKPYLLREY
                     QNRWYVVGVVRDTKKLRIFGIDRITDLEVKAETFDPGLIPDPASLFDHTIGLFLSQNT
                     LQTVVLSFTPTQGKYVKSLPLHKSQEILVDNGQECRIRLYVIPNYELTQEILRLGDTV
                     KVLEPEWLATEIRQILKKTLKQYK"
     gene            2654..2824
                     /locus_tag="PLM68_08210"
                     /pseudo
     CDS             2654..2824
                     /locus_tag="PLM68_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            complement(2880..>3280)
                     /locus_tag="PLM68_08215"
     CDS             complement(2880..>3280)
                     /locus_tag="PLM68_08215"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HOT55376.1"
                     /translation="SSQPVEVPTAEPAVSSQPAIVSAREQEAVPVRDEDEVTAKKKVD
                     LTQYAGNYYSPDIETIYRLFVQDGQLKVCRRAEDVFTLQHLSGDNFLSEGNGEFQFER
                     NRKGRVTGFEVSVSRAANVPFSLIPAGFEL"
BASE COUNT          898 a          751 c          784 g          847 t
ORIGIN      
        1 cgctgctggt gctgtcggct acgctgctgg tgctgtcggc tacgctgctg gtgctattgc
       61 ctacgctgct ggatcttccg gccgtgctgc tggtgctgtc ggctacgctg ctgcctgcat
      121 gcatatggta cccgcagagc aggaaaggca gcaatatcag gattgtcctt acattatttt
      181 ttttcattat tgggcgtgat ctttcgggtc agccattttc tcacaggaac gtcataaata
      241 cggtatattc cgtaagacaa ggctatggtt aggaaaaaga taaaaagagc cataggaaca
      301 gaggctttca tggatacctg gtttttgaat acccaggcca tgaacatata tatcagagga
      361 taatggatta tataaacggg ataggatatg tctcccagaa accggcagag ttttgtcgaa
      421 aaacgtcctg tcacgtttcc gctggctccc atatagataa tcaaaggaaa tagaatgatt
      481 atggaaaccg acacataaag tccattgatc caaatgtgtt ctgtgccgcc cagcctggga
      541 acggacagaa tagccacaag caacaggctg caccacagga aggcattttt cacactgcca
      601 ggtttcacca cgcgcgatag cagtaacccg gcaaagaagg ggtacaaaag acgggtaaat
      661 cctatacgta gctgaagcgg ctccagggac catcctccta tgatgtcgcc ccgggtgacg
      721 gcaaggtgga ctagcgctgc cccggaaatg acagccagta ctgcgagtgc ttttttggag
      781 aatttgtgaa tgaaaagtcc gtataaaata ttggctatgt attcaaaaaa aagcgtccag
      841 gccggcccgt tgagcgggaa cgtttcgttc catcccctca gatccagatt tggtcccacg
      901 ggaataagcg caaatcctaa taccataaca acaatcacct tccataaagg ggtctgggca
      961 atggcgggaa agatcgctgc ctgctggaag aaaagagtaa cagcgccaat aaacattccc
     1021 acaatgatca tcgggtgcag ccggataaga cggcgtttga aaaaattgcc aaggctcatt
     1081 tttccccacc ggtcatcata agcatatcca attacaaaac cggataatac aaagaagaag
     1141 tcaacggcca gatacccatg gttaatgagc tgtatatggt tgttgccatt ggtaaatgtt
     1201 tcaagcaagt gaaacagcac gaccaccagg gcggccacgc cgcgtaaagc atccagaatt
     1261 tcataatgtt ttttggtgtc cttaaaagtc gaagaaacgg gattcatttg tattgtttaa
     1321 gggttttttt cagaatttgc cggatttctg ttgccagcca ctcgggttcc agcaccttga
     1381 ccgtgtcgcc cagtcgcagg atctcttggg taagttcata gttggggatc acatatagcc
     1441 ttatcctgca ctcctgcccg ttatctacca ggatttcctg ggatttgtgc agtggcaagg
     1501 atttcacgta tttaccctgt gtgggggtga aggaaagaac tacggtctgc aacgtgttct
     1561 gggataagaa cagccctatg gtatggtcaa agaggctggc cggatccggt attaaacccg
     1621 ggtcgaaggt ctcggctttc acctcaagat ctgttatgcg gtcaattccg aaaatgcgca
     1681 gttttttggt gtcgcgcacc acacctacca cataccaccg gttctggtac tcgcggagca
     1741 ggtatggttt tactgtgtag tctgttgtct tgtctgtctg gaaattgtag tgcaggaaac
     1801 tgacctggcg ccgttccctc acggcacgca aaagcggttt cagattatgg atgcccttca
     1861 acccccctcc cgtgtcaaaa gaaatgtggc gcaaagcgtc cttgctttcc attaggctct
     1921 gtgtaagcaa ttctgccgta ttgacaatct ccaggaaatg gaaaaaagac tcaatgttaa
     1981 cgctttcttc gtagtctatg caataacccc tgcgggtgcg gtcgaatttt atctctaccc
     2041 cgaactcaaa gcggatctgt tccatatcgc gctgtatggt cctgtctcct acctgaaaac
     2101 catgctggtg aaggaagtcc cggatttccc ggaatgtggg gtaatgtccg ctcctgatct
     2161 tttctatgac cagggaatat cttcttattt ctgcgtgttt tgacatgatg cgggggttaa
     2221 tgagaagcaa atttattaaa tttcaatggt tctccataat gaagaagggt ggcaaacccg
     2281 gagtgccgcg caagagggat ttagcgtttt gcagaccgct gataatctga tatttatatt
     2341 tcagcaaatt ggcattgcgc ggcactccgg gtttgccacc cttgctgagc gcaaaatggg
     2401 ctcggcttga aaaaatgggc tcggcctgaa aaaagtttct gttcttgtgg tgtcagcgac
     2461 ctgaagtggc gtggaaggtc ccgcatgtta gtaaattggt gaaatatttg ttatctttaa
     2521 aagatttaca ttttgtgcag gttctgctct acgggtcctc cctcaccatc tgttaattaa
     2581 aaaaaggcca aagtcaccgt cgcattgagc taaaggtctt tgtatgtata gagttataat
     2641 taaatattaa taaatggcaa aatcagattc atttaacaaa agacaaatcg aaaaaaacaa
     2701 aagagctaaa cggaaagaaa aacaacaaaa aaaagaggag cggaaaaatg ctccaaaaag
     2761 ttcgtttgaa gacatgatcg cttacgtaga taaaaacggg atgatcacca gcacgcctcc
     2821 tgaaccagat aattctgaaa acaatacggt ttcagacgag acataggtgc ccgccaaaac
     2881 tatagctcaa agccagcggg tataagactg aaaggtacgt ttgcagcacg tgagacagag
     2941 acctcgaatc cggtcaccct gcctttccgg ttcctttcaa attgaaattc tccattccct
     3001 tcagaaagaa aattatctcc agaaaggtgt tgcagggtaa aaacatcttc tgccctgcgg
     3061 catactttta attgcccgtc ttgaacaaaa aggcggtata tggtttcaat atcgggacta
     3121 taataattcc ccgcatattg cgtaaggtct accttctttt ttgccgtcac ctcatcttca
     3181 tcccgtaccg gaaccgcctc ttgttcccgg gccgagacta tcgcaggctg cgatgaaacc
     3241 gccggttcag cggttggaac ttccactggc tgcgatgaaa
//
LOCUS       DAOSOF010000174         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29628_length_3250_cov_4.146479, whole genome shotgun sequence.
ACCESSION   DAOSOF010000174 DAOSOF010000000
VERSION     DAOSOF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29628_length_3250_cov_4.146479"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2116)
                     /locus_tag="PLM68_08220"
     CDS             complement(<1..2116)
                     /locus_tag="PLM68_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT55377.1"
                     /translation="MNISELSIKRPVLATVTVLIITLFGLIGYTFLGTREYPSVDQPI
                     ITVNTSYPGANADVIMNQITEPLEQNINGIPGIRSLSSVSSQGSSRITAEFELSVDLE
                     TAANDVRDKVSRAQRFLPRDCDPPTVSKADADATPIMQISVHSDTRSLMEVSEIADLT
                     VKEQLQTIQDVSGVSIWGEKRYSMRLWLDPARMAGYGVTPLDVKNAVDRENVELPAGR
                     IEGDKMELSIRTLGLMTTPHEFNNLIIKQDRGRIVRFQDVGRAELGPEDTRSAFRKDG
                     VPMVVVVIIPQPGANHIMIADQAIERIRQMKKDLPEDVSVDVLYDNTQYIRASIKEVR
                     DTIFIAFFLVVLVIFIFLRDWRVTLVPVVVIPVSLVGAFFVMYLAGFSINVLSMLAVV
                     LSVGLVVDDAIVVTENIYIRVERGMTPVEAGIEGSKEIFFAVVSTTITLVAVFLPIVF
                     MEGMTGRLFREFSIVIAGSVTISSIVALTFTPMLATKILKKRDSKGWFYRKTEPFFNG
                     MTNGYTRWLTAFIKVKWVSIPLVVLLFAAIILYWTAIPSELSPMEDRSQFTVRVTGQE
                     GSTFEYVRDITDQVASQADSIAYERKALTSRSSTGSGFVTVMLPDIDQRERSQMEIAE
                     AVTQQVIRESRARVIVQQTSTFGGRRGGMPVQYVLQAPTIEKLQEVLPRFMEEVDNSP
                     VLRMADVNLKFTKQELRINIDRD"
     gene            complement(2113..3219)
                     /locus_tag="PLM68_08225"
     CDS             complement(2113..3219)
                     /locus_tag="PLM68_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT55378.1"
                     /translation="MSKRTRWILVALVFLLLGGLVFFPTIRKIFFKNESGVVPGGEGL
                     RQRVLNVNAEVLTTHPMSDYTVTTGDILPAEEVDLTFETSGKVVDIHFQEGAYVTKGT
                     VLAKINDAPLQAQLKKLEAQVKLAQDRVYRQQTLLERDAVSQEAFESVRTDYNKLQAD
                     IELVKAQIAQTELKAPFDGIIGLRQLSEGAFASPGTKIATLAKVSELKIEFFVPETYA
                     LDIKRGTPVEFTLADNQGLERTFRASVYAVESGINRETRTLTVRALYANTGNQLVPGR
                     YVSVRILKEEIPNAVSVPAEAIIPEMGENLVYVYRSGKAQPVTIVAGLRTESRVQALT
                     GVKAGDTLITSGVMQLRSGLDVIIDQLTEGEGSL"
BASE COUNT          736 a          880 c          804 g          830 t
ORIGIN      
        1 tgtcccggtc aatgtttatg cgcagttcct gtttggtgaa tttcaggttc acatcggcca
       61 ttctcaatac cgggctgtta tctacctctt ccatgaaccg gggtagcact tcctgcaatt
      121 tttcaatcgt aggagcctgc agaacgtatt gcacgggcat ccctccgcgg cggcccccga
      181 aagtggaggt ttgctgcaca ataacacggg cacggctttc ccggattacc tgttgggtaa
      241 ccgcctcggc aatctccatc tggctgcgct ccctctgatc gatatcggga agcataacgg
      301 taacaaaacc gcttccggtc gatgaacgcg atgtaagggc tttcctttca taagcaatgg
      361 aatctgcctg gctggcaacc tggtctgtta tgtcccgtac atattcaaaa gtacttcctt
      421 cctgtccggt cacccgtaca gtaaactgtg accggtcttc cataggggag agttcggaag
      481 gtatggctgt ccagtacagg atgatggccg caaaaaggag aacaaccagt ggaatggaca
      541 cccattttac ttttataaaa gcggtaagcc accgggtata cccgtttgtc ataccattaa
      601 aaaagggctc tgtcttcctg taaaaccatc ctttactatc cctttttttg aggatctttg
      661 tggccagcat gggggtaaag gttagcgcaa caatagaaga aatggtaaca gatcctgcaa
      721 tcacaatgct gaattcacga aacaaacggc cagtcatgcc ttccataaaa acaatgggca
      781 ggaatacggc aaccagggta atagtagtgg agaccactgc aaaaaagatc tctttggacc
      841 cttctatgcc ggcttctacc ggggtcatgc cccgttccac gcgaatgtat atgttttccg
      901 taaccacaat ggcatcgtcc accaccaatc ccacagacag cactacggcc agcatggaca
      961 gcacatttat ggagaatccg gccaggtaca taacaaagaa agcccctacc agggaaacgg
     1021 gtatgaccac tacgggcacc agggtgacgc gccagtcgcg cagaaaaatg aatatgacca
     1081 gaaccaccag gaaaaacgct atgaatatgg tgtcacgtac ttccttgatc gaggcacgga
     1141 tatattgtgt attgtcgtaa aggacatcaa ccgacacatc ctccggaagg tcttttttca
     1201 tctgcctgat acgctcaatg gcctggtctg caatcatgat atgattggct ccgggctgcg
     1261 gaatgatgac caccaccacc atgggtaccc cgtccttgcg aaaagcactc cgagtgtcct
     1321 cgggtcccag ctcggcccgt cctacgtcct gaaagcggac aatgcgtccc ctgtcctgct
     1381 taatgatcag gttgttgaat tcatgaggcg tggtcatcag ccccaaggtg cggatggaaa
     1441 gttccatttt gtccccttca atccttcctg caggcagttc aacattttcc cggtccacag
     1501 cgtttttcac gtccaggggg gttaccccgt acccggccat ccgggccgga tccagccaca
     1561 ggcgcatgga atagcgtttt tctccccaga tacttacccc gcttacatcc tgtatggttt
     1621 gcaattgctc tttgaccgta aggtcggcaa tttcgctcac ttccatcagg gaccgggtgt
     1681 cgctatgaac gcttatctgc atgatgggcg tggcgtctgc atctgctttt gaaacggtgg
     1741 gcggatcaca gtccctgggt aaaaagcgct gcgcccgcga gaccttgtcc cggacatcat
     1801 tggccgctgt ttccaggtcc acgctcaatt caaattcagc cgttatgcgg gaactcccct
     1861 ggctgctcac actgctcagg gaacgaatcc cgggaatgcc gttgatgttc tgttccaggg
     1921 gctctgtgat ctggttcatg atcacatcgg cgttggctcc cggatagctt gtatttacgg
     1981 taattatggg ctggtctacg ctggggtatt ccctggtccc caggaaggtg tagcctatca
     2041 gcccgaacag ggtgatgatc agaacagtaa ccgttgccag gactggcctt tttatactga
     2101 gttcggaaat attcataaac tcccctctcc ttctgtgagc tggtcaatga tcacatcaag
     2161 gccgctgcgc agctgcatga ccccggatgt gatcagggta tcccctgcct tgacaccggt
     2221 aagggcctga accctgcttt cggttcgtaa tccggcaaca atggtgacgg gttgtgcttt
     2281 gccgctcctg taaacgtata ccaggttttc tcccatttcg ggaatgatgg cctcggccgg
     2341 aacagaaacg gcattgggaa tttcttcctt cagtatcctg accgagacat accgccccgg
     2401 gaccagctgg ttgcccgtgt tggcgtacag tgcccgtact gtaagggtac gcgtctctct
     2461 gtttatcccc gattccacgg cgtaaacact ggccctgaaa gtcctttcca gcccctggtt
     2521 gtctgccagg gtaaattcaa cgggggtgcc tcttttaata tccagggcat acgtttcggg
     2581 aacaaaaaat tctattttca attccgagac tttcgccagg gtggcgattt ttgtcccggg
     2641 cgaagcaaaa gccccttcgc tcagctgccg cagccctatg atcccgtcaa agggcgcctt
     2701 cagttcagtc tgtgcaatct gcgcttttac cagttctatg tctgcctgca gtttgttgta
     2761 gtctgtgcgt accgattcaa aagcctcctg gcttacggca tcgcgttcca ggagcgtttg
     2821 ttgacggtat accctgtcct gtgcgagttt cacctgtgcc tcgagttttt tgagttgcgc
     2881 ctggagcggg gcgtcgttga ttttggccag gacagtgcct ttggtaacat aagccccttc
     2941 ctggaaatga atgtccacta ccttacctga agtttcaaag gtcagatcca cttcttctgc
     3001 cggcaggata tcccctgtgg ttaccgtgta atcagacatg ggatgagtgg tgagcacttc
     3061 tgcattcacg ttcagaaccc gctgacgcaa cccttccccg cctggaacaa ctccggattc
     3121 atttttaaag aaaatcttcc tgatggtggg aaagaaaaca aggccgccca gcaatagaaa
     3181 tacaagagct accaggatcc atcgtgtacg ttttgacatg ttcgtttgtt cttaattgaa
     3241 ttgggaaaca
//
LOCUS       DAOSOF010000175         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29665_length_3248_cov_4.571250, whole genome shotgun sequence.
ACCESSION   DAOSOF010000175 DAOSOF010000000
VERSION     DAOSOF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29665_length_3248_cov_4.571250"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLM68_08230"
     CDS             complement(<1..931)
                     /locus_tag="PLM68_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT55379.1"
                     /translation="MKKITILILTGLFWGACSPEAIGQYGTIQLNQTNAEQVLEAMTL
                     KEKVSLLTGEGLIGSSIGAAGATKKLSRQGIPSLELSDGPAGLRLRSTSTTWYPSASL
                     LASTWDTSLISAVGKAMGAEALAHGADVILGPAMNIHRHPLCGRNYEYFSEDPLLTGI
                     AASAMVRGIQENGTAACLKHFAANNQETNRDNNNVIADERTLREIYLRGFEIAVKESA
                     PKTIMSSYNRINGTYTSEDSLLLTTILRDQWDFQGLVMSDWFGGRNAPAQIRAGNDLL
                     EPGRNWQRRAILKAVRKGELAEETINTAAGRVLR"
     gene            1006..2391
                     /gene="hisS"
                     /locus_tag="PLM68_08235"
     CDS             1006..2391
                     /gene="hisS"
                     /locus_tag="PLM68_08235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT55380.1"
                     /translation="MTQTPSIPKGTRDFNPLEASRRNYIFDTIRNVFKEFGYAPIETP
                     AMENLSTLLGKYGEEGDRLLFKILNTGDSFVPLAGKVDPNPANIDSNALALQVCEKGL
                     RYDLTVPFARFVVQHQNELTFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSDSL
                     YNEFEMVQLVDDIFNRFGIRVQLRINNRKVLAGIAQVMGYPDRLIDITIAMDKYEKNG
                     LEGVRIELLEKGITPEGLSRLEPLLLFKGVTTSKIAFLTRLFEEGGSEIGLKGVEELK
                     WLFSMIEKVGTGQAVELDLTLARGLNYYTGAIFEVKALDFSIGSICGGGRYDDLTGVF
                     GLKDMSGVGISFGADRIYDVLMGMGKFPESVMDAPQIVFFNMGRNEAAYALRCMKRLR
                     ASGITCELYPEEVKLKKQFEYAQRRGVRKVAIIGEQEMNNNQVTWKNLETGEQQTVDM
                     QDFLNMFGVLA"
     gene            2417..2489
                     /locus_tag="PLM68_08240"
     tRNA            2417..2489
                     /locus_tag="PLM68_08240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2450..2452,aa:Met,seq:cat)
     gene            complement(2532..2921)
                     /locus_tag="PLM68_08245"
     CDS             complement(2532..2921)
                     /locus_tag="PLM68_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55381.1"
                     /translation="MKKWVPLFTMTLLLCSAVSCTETPSETKTSYSWQLILEKPSSVP
                     IGLQIWKYADFFNDGTFVLSVDYYHGAQDTDTFQYSDSGNVIRIYDMYGKDWIGTMPS
                     GAIQAGNTVQFKAESGEGYFALKYLGN"
BASE COUNT          804 a          754 c          836 g          854 t
ORIGIN      
        1 gcctgaggac gcgaccggca gccgtattga ttgtttcttc cgccaattcc ccttttctga
       61 cggctttgag gatggctctc cgttgccagt ttctgccggg ttccagcaag tcatttcctg
      121 cacgtatctg tgccggggca ttccggcctc cgaaccagtc gctcatcacc agtccttgaa
      181 aatcccattg gtcacgcaat attgtggtga gtaacaggct gtcctctgac gtataggtcc
      241 cattgatacg gttataggaa ctcatgattg ttttgggtgc agactccttt accgcgattt
      301 caaagcctct gagatagatc tcacgcaacg tcctctcatc tgcgatcacg ttgttgttgt
      361 cccggttggt ttcctggtta ttggctgcaa aatgcttgag acaggcggcg gtgccgtttt
      421 cctgtatgcc acggaccatg gccgaagcgg caatccccgt gagcaaggga tcttccgaga
      481 agtattcata attccttcca caaaggggat gacggtggat gttcatggct ggccccagga
      541 tcacatcggc accatgggcc aaagcctcgg cccccatggc ctttcccacc gccgagatga
      601 gggaggtgtc ccaggtggaa gccagcaaag aggctgatgg gtaccaggtg gttgaggttg
      661 atctgagccg caggccggcg ggaccatctg agagttccaa tgaagggatt ccctgacggg
      721 acagcttttt ggtggcaccc gctgctccta tggaagagcc aataaggcct tctcctgtga
      781 gaagggaaac cttctccttc agggtcatgg cttccaggac ctgttccgca ttggtttggt
      841 tcagttgtat ggtcccgtac tgaccgatgg cctcagggga gcaggcgccc caaaaaagcc
      901 ccgtgagtat aagaattgta atctttttca tacccataaa tatcgcccat ttttttcaaa
      961 gtcgattgat ttcacttatt tttgcaaaga ccaatacaga atatcatgac acaaacacct
     1021 tccattccca aagggacacg cgattttaac ccgctggaag cttcccgtcg caattacatt
     1081 tttgatacca taaggaacgt tttcaaggaa ttcggttacg cacccattga aacgcctgcc
     1141 atggagaacc tttccaccct cctgggaaaa tatggcgaag aaggggaccg tctgcttttc
     1201 aaaatactca atacgggaga cagcttcgta cctctggccg gaaaggttga tcccaatccg
     1261 gccaacatcg attccaatgc cctggcattg caggtatgtg aaaaaggttt gcggtatgat
     1321 ctgacagttc cttttgcccg ctttgttgta cagcatcaga atgaactcac ctttcccttc
     1381 aagcgatacc agatacagcc tgtttggagg gccgacaggc cccagaaagg tcgttaccgt
     1441 gaattttatc agtgtgatgc cgatgtcatc ggatcggatt ccctctataa tgaatttgaa
     1501 atggtacaac tggttgatga tattttcaac cgttttggca ttcgtgtcca gctcaggatt
     1561 aacaaccgaa aggtgctggc agggatagca caggtgatgg gttatcccga taggttgatc
     1621 gacataacca tagctatgga taaatacgag aagaacggcc tggaaggtgt ccgtattgaa
     1681 ttgctcgaaa aaggcattac cccggaaggc ctgtcacgcc tggaaccgct gcttctgttc
     1741 aaaggcgtca ctacatccaa gatcgctttc ctgacacggc ttttcgagga aggtggttcg
     1801 gagatcgggc tcaaaggtgt ggaagagttg aaatggttgt tttccatgat tgaaaaggtt
     1861 gggacagggc aggctgttga actggacctg accctggcac gcggactgaa ttattatacc
     1921 ggagccattt ttgaggtgaa agcactggat ttctccattg gcagcatttg cggtggcgga
     1981 aggtacgacg acctgacagg tgtgtttgga ttgaaggaca tgtcgggtgt gggcatttct
     2041 ttcggggcag acaggatcta tgacgtgttg atgggtatgg gtaaattccc tgaatcagtc
     2101 atggatgcac cgcaaatcgt ttttttcaac atgggcagga acgaggctgc ctacgcgctg
     2161 cgatgcatga aacgtttacg tgcgtcgggc ataacctgtg agttgtatcc cgaggaagtc
     2221 aagctgaaaa aacagtttga gtatgctcag cgaagagggg tccggaaggt agccataatt
     2281 ggggaacagg aaatgaacaa caaccaggtg acctggaaaa acctcgaaac gggcgagcag
     2341 caaactgttg atatgcaaga ttttttaaat atgtttggag tattggcgta atttttgtac
     2401 atttgcaatt ctatatcgcg gagtggagca gttggtagct cgttgggctc ataacccaaa
     2461 ggtcggaggt tcgagtcctc cctccgctac gaaggataag ccagtcattt gactggtttt
     2521 tttcttttga atcagttgcc caaatatttc aatgcaaaat acccttcacc actttcagcc
     2581 ttaaactgta ccgtattacc agcctggatt gcgccggaag gcattgtccc gatccagtcc
     2641 ttgccataca tatcatagat cctgatcaca ttgccggaat cgctgtactg gaaagtatca
     2701 gtgtcctgtg ctccatgata atagtctaca gaaagtacaa aagtgccatc attaaagaaa
     2761 tctgcatact tccatatctg aaggccaatc ggaactgatg aaggcttttc caggatgagt
     2821 tgccagctgt aggaggtttt tgtttcggaa ggagtttctg tgcatgacac cgcagaacat
     2881 aataaaagtg tcatagtaaa aagggggacc cattttttca ttattaggga atatttgaag
     2941 ttagaaatac ataacaaagt taagaaattg acggcaataa caaaagaacc gggagctgtt
     3001 ttggtacacc ggggcttcaa ccctttcctg caggcggtcg cctgagtgcc aagaacggag
     3061 tgctttttcc ttaagccgag gcactccgtt cttagcactc aagcgacctt tcagttatgc
     3121 gtattgctgc cccaacaatc gcctggtggt cttcaaggcg gtgcgccaaa ggcttttggc
     3181 gcatataata tgctgcttcg cagcaccaat cattgttccc ggctgctttt tgggaggtag
     3241 taagtgat
//
LOCUS       DAOSOF010000176         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29827_length_3233_cov_5.119887, whole genome shotgun sequence.
ACCESSION   DAOSOF010000176 DAOSOF010000000
VERSION     DAOSOF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29827_length_3233_cov_5.119887"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLM68_08250"
     CDS             <1..261
                     /locus_tag="PLM68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF040149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55382.1"
                     /translation="IKYTNNIQNIIVLIYRSPHKFNGHAIGFYMSSQIVKAGFEDEMV
                     INFYNPYKFYTLYNLAAKNNGTFLFCNEFMEFLKNRTEKYYH"
     gene            complement(771..>3233)
                     /locus_tag="PLM68_08255"
     CDS             complement(771..>3233)
                     /locus_tag="PLM68_08255"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOT55383.1"
                     /translation="TQSQNDELIVTQKEFNTGGEASQLKIPVKTNIQYECSVQGNAGE
                     WITVFETQTKGLEEYFLILEIAQNNNYTERVGQVKITGAGKESYITITQDMRDEIKIM
                     YQKQRASLSALFKSLNGNQWKKKDNWDSDKPLDQWFGLTVDEHGFVTKLELPENNLKG
                     TLPAEIGDLVKLQSLYLGYNYGIGGSIPSEIGNLKELGFLNLFGNELTGEIPEELYSL
                     SELFHLDLGVNKLSGGLSSGIARLSKLLALWLHYNNFSGPLPKELGQMNLTQLALYNN
                     SFSGSLPDELGSLNNLDFFSVGSNKLSGFIPISILNNPNIWEKFWINITAFNDFDLSN
                     LTYYPAPDYTLTDVEGNSIDLGEEYASHQYTVLYSWTTWCPYASGFHPTLISLYNQYK
                     SKGLEVIGYYAQDQVADEIGMTDQKLKEAADYIGMPWSTVYLTTKYKAFGSALIQGGS
                     PTIHVVDKLGNIVFSTFAFADSRNDLGKFLEERLGEGSDYSYYASTDYSADGKVRTYQ
                     THTTGDGIKLIFTGDAFTDRDHADGTFDRRMNQAVEAFFSEEPYASLRNRFDVYSVAA
                     VSKNREIADDSETAFGTYFGSGTLVGGDDEEVFKYALKVPGVDMTTSLVTVVVNSPNY
                     SGTCYMYEDNAAVCYSAITGFSEGEFAKLIRHESGGHGFGKLLDEYAYSGTITDSKKS
                     DFIYKRDVLHWGGNVDVTSDPSKIQWSVMLSDARYKDLVEIYEGALTFAKGAWRPTYN
                     SIMHLNTEGYNAPSRFSIYKRIILLSENREATFDEFAEFDAPNLSKTTARPATTERTE
                     PLAPPVFIRGSWKDAGKRNIEK"
BASE COUNT          866 a          744 c          669 g          954 t
ORIGIN      
        1 attaaatata caaataatat ccagaatatc atagtgttaa tttatagaag tccccataag
       61 tttaatggtc atgctatcgg attttatatg tcaagccaaa ttgtaaaagc agggtttgaa
      121 gacgaaatgg taataaattt ttataaccct tacaaattct atacgttgta taaccttgcc
      181 gcaaagaata atggtacgtt tttgttttgt aatgaattta tggaattttt gaagaataga
      241 acagaaaaat actaccacta atgtataaag atcaaccgga tcacaagcat aagggggggg
      301 ggtgactaat aagtagttac ccccactttt atataagacc accggacggt cgtttgtgca
      361 gcaatgctgc ttaatggcag acttacagca aatcaggaag gtgctgctgc gcagcatatt
      421 ataagcgcca aatgcgcttg gcgcaccatt ctgaaagaca gcgttataga gtcgctgcac
      481 cacaaagccc cgggagcttt ttgggaacgc cgaggctccc ggttctttgt ggtggcaacg
      541 acagcagcag ctcagagttg agtgccttgc acacattttt ttggaggtaa acccggttta
      601 ccgcgcaacg cctgtttgca aaaatataaa tgtctgaata taaacgatct ataaaatgcc
      661 aaatccctct tgcgcggcac tccgggtttg cctccaacgt ttaaaaaata tccagaaacc
      721 tcggcgtacc aaaaagctcc tggatattta ttgtagcagc aaccgccggg ttatttctca
      781 atatttcgct tccccgcatc cttccagctt cccctgataa ataccggggg tgccagtggt
      841 tcggtccttt ctgtagttgc cggtctggct gtagtcttag ataggttggg cgcatcgaat
      901 tcggcaaatt cgtcaaaagt ggcttccctg ttctcggata gtagtattat ccgcttgtaa
      961 atggaaaatc tggatggtgc gttatagcct tctgtattca ggtgcataat actattgtag
     1021 gtcggcctcc aggctccttt ggcaaatgtc aaagcgcctt catagatttc cacaagatct
     1081 ttataacgtg catcgctcaa catgacagac cattgtatct tggacgggtc tgaagtcaca
     1141 tccacattcc ctccccagtg cagcacatct ctcttgtaga tgaaatcact cttcttgctg
     1201 tctgtaatgg ttcctgaata ggcatattca tccaggagtt tcccaaatcc gtgcccaccg
     1261 ctttcatgcc ttatgagctt ggcaaattca ccttctgaaa atcccgtgat agccgaataa
     1321 caaacggcag cgttatcttc atacatatag catgtacctg aatagttggg cgagttgacc
     1381 acaacggtaa ccagggatgt tgtcatgtca acgcccggaa ctttcaatgc atatttgaat
     1441 acttcttcat catctcctcc taccaatgtt ccacttccga aataggtacc gaaggcagtt
     1501 tccgagtcat ctgcaatctc gcggttctta gaaactgctg caacggagta gacatcaaaa
     1561 cggttacgta aagaggcata gggttcttca ctgaagaaag cttccacggc ctggttcatt
     1621 ctcctgtcaa atgtgccgtc tgcatggtcc ctgtcggtaa aagcatctcc ggtgaaaatg
     1681 agtttgatgc cgtctccggt tgtatgtgtc tggtatgtcc tgactttccc atctgcagaa
     1741 tagtccgttg aagcatagta gctatagtct gaaccttcac ccagtctttc ttcgaggaat
     1801 ttaccaaggt cattgcgact atctgcaaat gcaaaggtag aaaatacaat gttgcctaat
     1861 ttgtctacaa cgtggatggt tggtgaccca ccttgtatta acgccgagcc aaatgcctta
     1921 tacttcgtgg tcagataaac tgtactccag ggcataccta tataatctgc agcttccttc
     1981 agcttctgat ctgtcattcc aatttcgtca gccacttgat cctgagcata ataaccaatc
     2041 acttcaagtc ccttggactt atactggttg taaagagaaa taagtgtcgg gtgaaaaccg
     2101 ctggcatacg ggcaccatgt agtccatgaa tacaagaccg tgtactggtg tgatgcatac
     2161 tcttcgccta aatcgatact gttgccttca acgtcagtta acgtgtaatc gggagcagga
     2221 tagtatgtca ggttggaaag atcaaaatca ttaaaggctg taatattaat ccagaatttt
     2281 tcccagatat tgggattatt aagaatagag attggtatga agcctgataa tttattggag
     2341 ccaacgctga aaaaatcaag attattcaaa cttcccagtt catcgggcag tgaaccggag
     2401 aatgaattat tgtacagggc gagctgtgta agattcatct gtcccagttc ttttggcagt
     2461 ggtcccgaaa aattattgta atgcagccat aacgccaaca gcttacttaa tcgggcaatt
     2521 cctgatgaca atccgccgga aagcttgttt acgcccaggt ccaaatggaa caattcagac
     2581 agggaataca gttcttccgg tatctctccg gtcagttcgt tcccaaacag attcagaaac
     2641 cccagttcct tcagattccc tatttctgag ggaatactac caccaattcc atagttatat
     2701 cccagataaa gcgattgaag cttcaccaaa tctccgattt cggcaggcaa agtccctttg
     2761 aggttgtttt caggcagttc cagtttggtg acaaatccat gttcatctac tgtcaaacca
     2821 aaccattggt caagaggctt gtcagaatcc cagttatctt ttttcttcca ctggttcccg
     2881 tttaatgact tgaataatgc cgaaagggat gccctttgct tctgatacat tatttttatc
     2941 tcatcacgca tgtcctgtgt gatggtgata tagctctctt tcccggcccc tgtaattttg
     3001 acctgaccta ccctttctgt gtagttattg ttttgggcaa tctccagtat taggaagtac
     3061 tcttccaaac ctttggtttg tgtttcgaag accgtgatcc attcgccggc attcccctga
     3121 acactgcact catactgtat atttgttttc acagggatct ttaactggct tgcctcaccc
     3181 cctgtattga attctttttg agtcacaatc agctcatcat tctggctctg cgt
//
LOCUS       DAOSOF010000177         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_29981_length_3219_cov_4.489570, whole genome shotgun sequence.
ACCESSION   DAOSOF010000177 DAOSOF010000000
VERSION     DAOSOF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29981_length_3219_cov_4.489570"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLM68_08260"
     CDS             complement(<1..941)
                     /locus_tag="PLM68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOT55384.1"
                     /translation="MNKIILTLGLLAALTLAGCSDFLDRPQKTAMDDSNYWTSETNIR
                     LFVNGGYPNYFNGYDRSWGTAYVPYRGANFNDDMTSAGKQSAFLAAVPADNWYRSESA
                     TTIYWLTQSGASAWNFGWVRKWNTLIQRLAKMKEEGYLSNEQFNHWNGVARLFRGMEY
                     SRLVQSFGDVPYYDKPVSDTDMDEQYKDRDPRTLVMDKVYEDFVYAIANIRANDGVNY
                     VNKYVAAALASRYMLFEGTWYKYHPGSGTNDLAKKFLQLVVSAGDVVINSGQYAFTTD
                     FRSLFGSDTKQGKEILLYREYSAELSTTHCIASYSNLT"
     gene            complement(953..>3219)
                     /locus_tag="PLM68_08265"
     CDS             complement(953..>3219)
                     /locus_tag="PLM68_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT55385.1"
                     /translation="ASARVSSQINKFLSVHAGLMYSKTQKRWAYATSSTTADIWYYMY
                     RWGPTYPMAASDESGNPLRNATYETAVANTASITNNFTSANAGLTITPVKDWNINLDY
                     TYSNQESIELNPGTSFMAGDTWVSAVPLNNQDGTRIYRDNEWNQHNSLGASIPAYMLN
                     YSRYTAPGSNPDHIYRSAANRTRSTLNLTTTYDLNINDAHIFNFMLGMNAVAYDYNYS
                     WSQKLQLLDYTNPQFDLASGTQTSGGNSLWDSQLGFFGRINYNFKERYLFEANLRYDG
                     TSKFPKDLQWRWYPSFSFGWRVMEEPWMDFAKPALTSLKLRGSWGSIGDQSVSNTLYV
                     PTMSGANSYWLHGGARDTYFGTPAAVSSIITWQDIVTLNVGVDISILNGALSASLDWY
                     QRDTKNMIVPGEGFSYTFGTTAPQGNFGSLRTNGWEVSLNFGHQFASGFRITASAAFS
                     DAVTHITKYGSTTSINAWYEGKVYGEIWGYQVDRLYQNDDFVYENGKLVTMKSTDGYT
                     VNKLSDPNAPTQGKLQTSSNFRFGPGDVKFKDLNGDGVINNGSQLIDDYGDLSVIGNT
                     TPRYEYSFRLGMEYKGVDFSVFFQGVGKRDMWGASPLAIPGFNSSDGAMPKVIAQDFW
                     YETYDGSGNVIDSNYDAFYPRAYNLGASSTGNNMQVCDRYLLNMAYLRMKNLTIGYTM
                     PRQLTRKAMINNVRIYISLENFLTFDKLRGLPIDPEEIAGYSLLNTSNYNSSRTGVGV
                     PTFKSASVGLQLTF"
BASE COUNT          808 a          746 c          784 g          881 t
ORIGIN      
        1 gtaaggttgg aatacgaggc tatacagtgg gttgtagata attctgcgct gtattccctg
       61 tacagcagga tttctttgcc ttgttttgtg tcagagccaa aaagcgagcg gaaatccgtg
      121 gtaaatgcat attgccccga attaatgacc acatcacctg cagacaccac taattgaagg
      181 aattttttag ccaggtcgtt agtgcccgag ccgggatgat acttgtacca ggtgccttca
      241 aagagcatat aacgggaagc cagtgccgct gcaacatact tgttgacata gttcactccg
      301 tcgtttgctc tgatattggc tatggcatag acaaaatctt catagacttt atccatgacc
      361 aaagtcctgg gatccctgtc cttgtattgt tcgtccatgt cggtgtcact tacgggttta
      421 tcgtagtatg ggacatcccc gaatgattgt acaaggcggc tgtattccat gcccctgaaa
      481 agcctggcaa caccgttcca gtggttgaat tgttcatttg agagataacc ctcctctttc
      541 atcttggcca gacgttgtat gagtgtgttc catttccgga cccatccaaa gttccaggca
      601 gatgctccgc tttgagttag ccagtaaatg gtcgttgccg attccgagcg gtaccagttg
      661 tcggcaggaa cggcagcaag aaaggctgat tgtttgccgg ccgaagtcat atcgtcgttg
      721 aaatttgcgc ccctgtatgg aacgtaggcg gttccccatg atctgtcata tccgttgaaa
      781 taattgggat aacctccatt tacaaacaga cgtatattgg tttcgctggt ccagtagttt
      841 gaatcgtcca tagctgtttt ctggggacga tccagaaaat cgctgcaccc cgccagggtt
      901 aaagccgcta ataaacctaa ggttagtatt attttattca tagtgtctga ttttagaaag
      961 ttaactgaag gccgacagat gcgctcttaa aggttggaac gccaacacct gtgcgggagg
     1021 aattgtaatt actcgtattt agtaaggaat atccggcaat ttcttccgga tctatgggaa
     1081 gtccgcgaag tttgtcaaat gtcaggaaat tttccaggga aatataaata cgtacattgt
     1141 ttatcatagc ctttcgggtc aattgcctgg gcatggtgta acctatagtc aggttcttca
     1201 ttcttagata ggccatgttc agcagatacc tgtcgcatac ttgcatgttg ttcccggtgc
     1261 tgctggcccc caggttgtag gcacggggat agaacgcatc gtaattgctg tcaatcacat
     1321 taccgctgcc atcatacgtt tcgtaccaga agtcctgggc tatgaccttg ggcatggctc
     1381 cgtctgaaga gttgaaaccg ggaatggcca gagggctggc gccccacatg tcacgctttc
     1441 caacaccctg gaagaatacc gagaaatcaa ctcccttgta ttccatgcca agacggaaac
     1501 tgtattcata acggggagtt gtgttcccaa tcaccgataa atctccgtaa tcgtcaatca
     1561 gctggctccc gttgttaatc accccgtcac cgttaaggtc cttgaacttc acgtcaccag
     1621 gcccaaatct gaagttggag cttgtctgca gcttaccctg tgtgggggcg tttggatctg
     1681 acaatttatt gactgtatat ccatcggtgc ttttcatggt tacgagttta ccattttcat
     1741 atacaaaatc atcgttctga taaagcctgt caacctggta accccagatc tctccgtaca
     1801 ctttcccttc gtaccaggca ttgatgctgg tggtgcttcc gtattttgta atatgagtaa
     1861 cagcgtctga aaatgcagca ctggcagtga tccggaatcc gctggcaaac tggtgcccaa
     1921 aattcaggga tacttcccag ccgtttgtcc gtaaagaacc aaagttgccc tgaggagcgg
     1981 ttgttccgaa tgtgtaggaa aatccttccc cgggaacgat catgttttta gtgtctcttt
     2041 gataccagtc taacgaagca ctcaatgctc cgttaagaat actgatatca acccccacgt
     2101 tgagtgttac aatatcctgc catgtaatga tggaggatac agcagcagga gtgccaaaat
     2161 aagtgtctct tgcccctccg tgcagccagt atgaattagc tccgctcatg gtgggcacat
     2221 acagggtgtt gctaaccgac tggtcgccaa tagaacccca cgatccgcgt aacttcaggc
     2281 ttgtcagggc aggtttggca aaatccatcc agggttcttc cattacacgc catccaaacg
     2341 agaaagaagg ataccagcgc cattgcaggt ctttggggaa cttggatgtg ccatcgtaac
     2401 gaaggttggc ttcgaaaaga tatctttcct tgaaattata gttgatacgc ccaaagaaac
     2461 caagctgcga atcccagagc gaattaccac cagaggtttg tgttccggaa gccagatcaa
     2521 attgcggatt agtgtaatcc aataactgta gcttctggga ccacgaataa ttataatcat
     2581 acgccacagc gttcatcccc aacatgaaat tgaagatgtg ggcatcgttg atgttcagat
     2641 cataggtggt ggtcaggttc agtgtgctgc gggtacggtt ggctgcagac ctgtaaatgt
     2701 ggtccggatt cgaacctgga gccgtatacc tggaataatt gagcatatag gccggaatgg
     2761 aagctcccaa tgaattatgc tggttccatt cgttgtccct gtaaattcgc gttccgtcct
     2821 gattattcaa aggaacagca gaaacccagg tgtcaccggc catgaaactt gtaccgggat
     2881 taagttcaat gctttcctga ttggaatagg tatagtccag attgatgttc caatctttca
     2941 ccggcgtgat ggtcaggccg gcattggcac tggtgaagtt gttggtaata gaagccgtat
     3001 tggccacggc agtctcatac gtggcattgc gaagcggatt gccgctctca tcggacgctg
     3061 ccataggata agtaggtccc catctgtaca tatagtacca gatatcggca gtggtagagg
     3121 atgtggcata agcccagcgt ttctgggttt ttgaatacat caggccggcg tgtacagaaa
     3181 ggaatttatt gatctgtgag ctgacccttg ccgaagcat
//
LOCUS       DAOSOF010000178         3199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_30187_length_3199_cov_7.225509, whole genome shotgun sequence.
ACCESSION   DAOSOF010000178 DAOSOF010000000
VERSION     DAOSOF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30187_length_3199_cov_7.225509"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLM68_08270"
     CDS             complement(<1..436)
                     /locus_tag="PLM68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOT55386.1"
                     /translation="MAQEIERKFLVAGPYKPLAVKSTRITQGYLNSVPERTVRVRVKG
                     DKGFITVKGIGNKSGASRYEWEKEIPVEDVRSLLELCEPGVIDKTRYLVKAGPHTFEV
                     DEFYGENEGLTLAEIELGSENEAFEKPSWLGAEVTGDTRYYNS"
     gene            complement(438..1082)
                     /locus_tag="PLM68_08275"
     CDS             complement(438..1082)
                     /locus_tag="PLM68_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55387.1"
                     /translation="MSKEKMEIGAISKPRFEFRSFGQNFDRAHQRMARLSVPVPEKVW
                     ERSSDEVYIISAANDINNTKIRDGKMDIKTYVQTVQGLEQWNPLMKGEFPISAKVLRE
                     EVFPAFMVEMPPLTKDTYTYDEFIEMVRKHPLLAAVRVHKQRFGYMVNDTICEVGNVF
                     VNGAKVVTINSESTEIEDILKTIREVGLEGVENINYLQAIKRIIGMSPKPLANE"
     gene            complement(1101..1919)
                     /locus_tag="PLM68_08280"
     CDS             complement(1101..1919)
                     /locus_tag="PLM68_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55388.1"
                     /translation="MKKILFAALLLVAGFTTSCVKDDLYDGPASISNLKFNPEAVTPK
                     DAVTVTATITDLRSVSSAVLKYSIGGGSAQSVNMTASGDVYSGIIPAQADKAVVTFHV
                     EAVNEKGYIAKSADKSYTVAAVVVDYSGLYLNELNGNDKFMELQNRGTKDIPLEGVYI
                     CKDSENDSPVWVCDDRTLAPGQFLLLYSEDVQSTHPEQPEALIFHSGLSAKKNVRIQL
                     FNPAGSSIDDFNLTAIAKTAPASYSRNTDKKWAYAPATPGAANQESTDYVTGLQ"
     gene            complement(1940..>3199)
                     /locus_tag="PLM68_08285"
     CDS             complement(1940..>3199)
                     /locus_tag="PLM68_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT55389.1"
                     /translation="EERNGVLVFESRNEDYKIWFDSRVQVDGAAFFGENKDYDPIGNG
                     VSIRRARFAVKAQVTKDWYGEVDLDFANGILELKDAYVMYSGIKNLEIQAGNFKEGFS
                     MESTTTSRYLPFMERPMVVQTFAPSRHIGAQLKTNLNWFMASAGVFFQAVEDAEIRTN
                     VEDNNKDYGRNSGYSVTAKVVAMPFYQDSNKGLHIGLAGSYRTPKVDMAPSEYGGVRY
                     STRTATSINRRKYLDTDVIRDVHHDLLYGAELAAYFGGLRMQGEYIGNITSLNNPVAT
                     VNGETSRLYFSGWYAHVGYLLFGGKQRYNTNDGEFTQPSRGRDWGDIEILFRYDYLTL
                     NSAPIYGGSGQNYSAGLNYYINNNIKIMLNYMYSDHDRFANGKGKLLVGHDASGAPTK
                     DYTKVVDSPRTAGVDYHTLSVRFEIDF"
BASE COUNT          701 a          807 c          805 g          886 t
ORIGIN      
        1 tggaattgta ataccgggta tcgcccgtga cttccgcacc cagccaggaa ggtttctcaa
       61 aagcttcgtt ttccgaacca agctcgatct cggccagagt gaggccctcg ttttctccat
      121 agaattcatc cacttcaaac gtgtggggcc cggcctttac caggtaccgc gttttgtcta
      181 taacacccgg ttcgcagagt tcaaggagcg aacggacatc ttccaccggg atttcttttt
      241 cccattcata acggctggca ccgctcttgt tgccgatacc cttgaccgtt atgaaaccct
      301 tgtctccttt caccctgacc ctaactgtcc tttcaggcac agagttcagg taaccctggg
      361 taatgcgggt ggacttgacg gctaacggct tatacgggcc agccaccaag aatttgcgtt
      421 ctatttcctg tgccatatca ttcgtttgcc agaggtttgg gactcattcc tattatgcgc
      481 ttgatggcct gcaggtagtt gatgttttca acgccttcca gtcccacctc cctgatggtt
      541 ttcaggatgt cttcaatctc ggtggattcc gagttgatgg ttaccacttt ggcgccattg
      601 acaaagacat tgcccacttc gcagatggtg tcgttcacca tatagccaaa acgttgtttg
      661 tgcactctga cggcagccaa aaggggatgc ttccggacca tttcaatgaa ttcgtcgtag
      721 gtataggtat cttttgtcag cgggggcatc tctaccatga aggcagggaa cacttcctcc
      781 ctgagcacct ttgcggaaat ggggaactct cccttcatga gcggattcca ttgttccagt
      841 ccctgtaccg tctgtacgta ggttttaatg tccatcttgc cgtcacggat cttggtattg
      901 tttatatcgt tggctgccga tatgatatac acctcgtcac tgctgcgctc ccacactttt
      961 tccggaacag ggacagacag tctggccata cgctgatgcg cccggtcaaa attttgcccg
     1021 aaggaacgga actcgaatct tggctttgat atggcgccta tctccatttt ttcttttgac
     1081 atatatttac tggcaatgtt ctattgcagc cctgttacat aatctgtact ttcctggttt
     1141 gcggcaccgg gagttgccgg ggcgtatgcc catttcttat cggtattgcg gctataggat
     1201 gccggggcag tcttggcaat ggccgtaagg ttgaaatcgt caatgctgct accggccgga
     1261 ttgaacagct ggattcttac attcttcttg gccgagagcc cgctgtggaa tatcagggct
     1321 tcgggctgtt ccggatgcgt tgactgcacg tcttcgctat agagcagcag gaactgaccc
     1381 ggagcaagtg tgcgatcgtc gcaaacccat acaggagaat cattctcact gtctttacaa
     1441 atataaacgc cttccagggg aatatccttg gttcccctgt tctgcaattc catgaattta
     1501 tcattcccgt tcagctcgtt caggtaaagt ccggagtaat ccacaacaac agcagcgacg
     1561 gtataggatt tatcggccga tttggcgata tatccttttt cgttcacggc ttcgacatgg
     1621 aaagtcacca ctgccttgtc ggcctgtgcg gggataatcc cggaatatac atcgcccgag
     1681 gcagtcatgt ttaccgattg ggccgagcct ccgccgatgg aatatttcag gacagccgag
     1741 gaaacgctac gcaggtctgt gatggttgca gtaacggtta ctgcgtcttt aggggtaacg
     1801 gcttcggggt tgaatttaag gttggaaatg gatgcgggcc cgtcgtacag gtcgtccttg
     1861 acacaggagg tcgtgaaacc tgcaaccaga agaagcgccg caaaaagtat ctttttcata
     1921 gttgttcttt ttttgagggt taaaagtcaa tttcaaaacg taccgacagg gtatgataat
     1981 caacaccggc tgtgcgaggg gaatcaacca cttttgtgta gtccttagtg ggggctccgg
     2041 aggcatcgtg gcccacaagc agttttccct tgccgtttgc aaagcggtcg tggtctgaat
     2101 acatataatt aagcatgatc ttgatgttgt tgtttatata gtaattcaaa cctgcggaat
     2161 agttctgtcc cgaacctccg tatatggggg cgctgttcag ggtcaggtaa tcgtagcgga
     2221 acaggatttc tatgtcaccc cagtcacgtc cgcgtgaagg ctgagtaaat tcaccgtcat
     2281 tggtgttgta gcgttgtttt cctccaaaga gaagatatcc cacgtgggca taccatccgc
     2341 tgaagtacag acgggaagtt tcaccgttga cagtggccac ggggttgttc agggaggtaa
     2401 tatttccgat gtattcaccc tgcatgcgca atcctccgaa ataagcagcc agttcggctc
     2461 cgtagagcag gtcgtgatga acgtccctga tgacatcggt gtctaagtat tttctgcggt
     2521 tgatggaggt ggctgtgcgt gtgctgtagc ggacaccgcc gtattcgctc ggggccatat
     2581 caactttggg tgtcctgtaa gaaccggcca gccctatgtg caaaccttta ttgctatcct
     2641 ggtagaaagg catggcaacc actttggccg tcacggaata gcccgagttg cgtccgtagt
     2701 ctttattgtt gtcttccacg ttggtgcgga tctcggcgtc ttccacagcc tggaagaata
     2761 caccggcaga ggccatgaac cagttcaggt tggttttcaa ctgcgcacct atatggcggg
     2821 acggtgcaaa agtctgtacc accatgggcc gctccatgaa cggcaggtaa cgcgaagtag
     2881 tggtggattc catggagaat ccttccttaa agttaccagc ctggatctca aggtttttaa
     2941 taccactgta catcacgtag gcatctttga gttccagaat accgtttgca aaatcaaggt
     3001 caacttctcc gtaccagtct tttgttacct gggctttgac ggcaaacctt gcccggcgta
     3061 tggaaacgcc gttaccaatg ggatcgtaat ctttgttttc cccaaagaat gcggccccgt
     3121 ctacctgaac ccggctgtca aaccagatct tgtagtcttc attcctggat tcaaatacca
     3181 ggacaccgtt gcgctcttc
//
LOCUS       DAOSOF010000179         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_30579_length_3168_cov_3.754899, whole genome shotgun sequence.
ACCESSION   DAOSOF010000179 DAOSOF010000000
VERSION     DAOSOF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30579_length_3168_cov_3.754899"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..2018
                     /locus_tag="PLM68_08290"
     CDS             198..2018
                     /locus_tag="PLM68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT55390.1"
                     /translation="MGLTDKLEYIDQLGATAIWLSPVHPAMSYHGYDVKDYSKTNPVY
                     GTMDDFKDFVRAAHQKDIKVYLDYVINHTGREHWWFSQAIRSSDNPYRDYYIFSDDPR
                     SDIIAGKIPMIRTEGASGYDSGQWFTVSTTGDKTLAFKLDWSNPAQPKLTVTETETAD
                     PDNDAPAADNAKYLYFGDGVIRQFHDKGGGQYELVTNYSSSWGFLIRTSTTQWNNKTK
                     YGAQSTSASMITYGKPFVLYTHDNPDNVYDLQLPGSTMFHSHFWTNWFADLNYGAVET
                     AEQSPAFKAIVEDAKVWIDAGIDGFRLDAVKHIYHNEFSDENPEFLKKFYKAVNDYYR
                     LSRNQDIYMVGEVFSGYEDVAPYYKGLPALFEFSFWHRLQWALGQNTGCYLAKDMFTY
                     QNLYKQYRTDYIRATKLSNHDENRARSDLGGSLAKSRQAGAILLTAPGSPYVYYGEEM
                     GYIGTKTRGDLYVRSPMYWGDSYTTSYTDEIDQALPPAVGTVLTQQADTASILNVYRR
                     FSRARNTYPALASGTMTEHPVYNDKASASFPQLAAWYLSGGGEKLLVLHNLGKDQVTF
                     ALNDAIDHAVVTLGNVYIQKEKETNKLKMDSYSSVVFKIK"
     gene            2026..>3168
                     /locus_tag="PLM68_08295"
     CDS             2026..>3168
                     /locus_tag="PLM68_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT55391.1"
                     /translation="MKSFSCLLLTGILVLLQACSPSVSRKSVFDVLEPATVLNLEQDT
                     TIVLVRDYFPSIDKVDRTDSPNLKVIPYQGQDTVMIILHEDTPAMTVLNVHTKGQVGN
                     IPVWRMTLQTQAAPRLAVFPASPDTDALFTVRIENGPATLYLFWQNSLVQTVRITDVY
                     NVLLPGFTALMDRSWVRVYAVNQGGHSNDLLIPLDKGRPVLRQDQITRSDKHARIMYQ
                     VFVDRFFNGNPANDRRINSPEVLPRVDYQGGDLEGVVLKIKEGFFRELGVNTIWLSPI
                     TQNPYDAWGQIEDPKTKFSGYHGYWPLYITKVDDRFGNEQVLTELLDRAHEDNMNVIL
                     DHVANHMHINSPTLQQHPDWVTSPVTPDGRPNFELWDEFRLTTWFDK"
BASE COUNT          867 a          775 c          768 g          758 t
ORIGIN      
        1 tcctttgcgc ccttttcctg ctctttgcat cctgtcgtaa ggaacccact ccgggaacag
       61 atccagacaa tggagcatag ttctccaggg ttaaggacac tccccgggcc tgggatgaaa
      121 acaagcgagc tgatatcacc tatcagctcc ttgtttattc atttgcagat aaggacggag
      181 acgggtgggg cgatataatg ggacttacag acaaactgga atatatagac cagcttggag
      241 ccactgccat atggctttca cccgtgcatc cggccatgtc ctatcacggt tatgatgtga
      301 aggattacag caagaccaat cctgtatacg gcacgatgga cgatttcaag gattttgtcc
      361 gggctgccca tcaaaaggat ataaaggttt acctggatta tgtcatcaac catacgggca
      421 gggaacactg gtggttcagc caggctatcc gttcttccga caacccgtac cgggattatt
      481 acatattttc ggacgatccg cgctccgata tcattgccgg taaaattcca atgataagaa
      541 cggaaggggc atctggttac gacagcggac agtggttcac cgtttccaca acaggtgata
      601 aaacccttgc attcaagctg gactggagca atcctgccca gccaaagctc actgtgacag
      661 aaacggaaac ggcagacccg gacaatgatg cccctgctgc cgacaatgcc aaatacttgt
      721 attttggtga tggtgttatc aggcaattcc atgacaaggg tgggggacaa tatgaattgg
      781 tgacaaatta ctcctcgtcc tggggctttt tgatacgcac cagtaccacc caatggaaca
      841 acaaaaccaa atacggggca caaagcacct cggcttcaat gatcacttac ggcaaaccgt
      901 ttgtccttta tacccatgac aatcccgaca atgtatacga tctgcagctg ccgggttcca
      961 ccatgttcca ctctcatttc tggacgaact ggtttgcaga cctgaattac ggagcagtag
     1021 aaacggcaga acagagtccg gctttcaagg ctattgtgga agatgccaaa gtctggatag
     1081 atgcaggcat tgacggtttc cgcctggatg ctgtcaaaca tatttaccac aatgaatttt
     1141 cagatgagaa tccggaattc cttaaaaaat tctataaagc cgtaaatgat tattacaggc
     1201 tgtccaggaa tcaggatatt tatatggtag gggaggtgtt ctccgggtat gaagatgtgg
     1261 ctccgtacta taagggactg cctgcccttt ttgaattctc tttctggcac cggctgcaat
     1321 gggccctggg tcagaacaca ggctgttacc tggctaagga tatgtttacc tatcagaacc
     1381 tttacaagca atacaggaca gattacatac gtgccacaaa actgtccaac cacgatgaaa
     1441 accgcgctcg ctcagatctg ggagggtcac tggccaagtc aaggcaggca ggtgccatac
     1501 tgcttaccgc tccgggatct ccctatgttt attacgggga agaaatgggc tacataggga
     1561 caaaaacacg cggtgatctt tacgtaagaa gccctatgta ctggggagac agctacacca
     1621 ccagttatac agatgaaatc gatcaggccc tgccaccggc cgtcggtacc gttcttacac
     1681 aacaggcaga cacagcttcc atactgaatg tttaccgaag gttttccagg gccaggaaca
     1741 cgtatccggc tctggcatca ggaaccatga ccgagcaccc ggtatacaac gacaaagcat
     1801 ctgcgagctt tccccagctg gctgcatggt acctttccgg aggcggtgag aagctgcttg
     1861 tcctgcataa cctgggaaaa gaccaggtta cgtttgccct taacgacgca attgatcatg
     1921 cggttgttac cctgggcaat gtatatattc agaaagaaaa agaaacaaac aagttgaaga
     1981 tggattcgta ttcatctgtt gttttcaaaa taaaataatg cgaccatgaa aagcttttcc
     2041 tgtttgcttc tgacggggat ccttgtcctt ttacaggcat gttcaccttc cgtttccagg
     2101 aaatctgttt ttgatgtgct ggaaccggca acagtcctta acctggaaca ggatacaacc
     2161 atagttctgg taagggacta ttttccatct atagacaagg ttgacaggac ggattcaccc
     2221 aacctgaagg tgatacctta ccagggacag gatacggtga tgatcatcct gcacgaggac
     2281 acgcccgcaa tgactgtgct caatgtgcac accaaaggtc aggtcggaaa cataccggtc
     2341 tggagaatga cattgcaaac acaggcagct ccacgtctgg ccgtttttcc ggcatcacca
     2401 gatacagatg ccctttttac cgtaaggatt gagaatggtc cggccacgtt atacctcttc
     2461 tggcagaaca gcctggtaca gacagtacgg atcacggatg tatacaatgt actgttgccg
     2521 ggattcactg cattgatgga caggtcatgg gtgcgggttt atgcagtaaa ccagggcggt
     2581 cattcaaacg acctgctcat ccctcttgat aagggccggc cggttttgag gcaggatcag
     2641 atcacccgca gcgataagca tgcacgaatc atgtaccagg tgtttgtaga ccgttttttc
     2701 aacggaaatc ctgccaacga tcgcaggatc aattctccgg aagtgcttcc cagggtagat
     2761 taccagggag gcgaccttga aggggtggtc ctgaagatca aagagggatt ttttcgcgaa
     2821 ctgggtgtca acaccatatg gctttccccc ataacacaga atccctatga cgcctgggga
     2881 caaatagaag atcccaaaac caaattcagt ggttaccacg gatactggcc tttgtacata
     2941 acaaaagtgg atgaccggtt tgggaatgaa caggtcctta cagagctgct ggacagggcc
     3001 catgaggata atatgaatgt tatcctggat catgtagcca accacatgca cataaacagt
     3061 cccactttac agcaacatcc cgactgggtt acatctcctg tgacgcccga cgggcgcccc
     3121 aattttgaat tatgggatga attcagactc accacatggt ttgacaaa
//
LOCUS       DAOSOF010000180         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_30840_length_3148_cov_4.747171, whole genome shotgun sequence.
ACCESSION   DAOSOF010000180 DAOSOF010000000
VERSION     DAOSOF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30840_length_3148_cov_4.747171"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLM68_08300"
     CDS             complement(<1..567)
                     /locus_tag="PLM68_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HOT55392.1"
                     /translation="MKKISLLILLMTTLAGTAAAQELRNFAFGRQPIVSPEITENQVT
                     FRLLAPKAYEVRLYGSWTADYRSSLAMARDTAGVWYASIPLPAPEIYTYSFIVDGVMM
                     NDPSNVIMQRDGTRYLSVLLVPGGISGYYKEATQRGNVRTVWYDSPTIGMQRRMTIYT
                     PYGYENNPRGKYPVLYLLHGAGGDEEAWI"
     gene            696..1229
                     /locus_tag="PLM68_08305"
     CDS             696..1229
                     /locus_tag="PLM68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOT55393.1"
                     /translation="MMVHNPWHSVEPEAKGFEVNALIEISNGSRAKYELDKETGLLKL
                     DRVLYSAVYYPANYGFIPRTYAKDDDPLDVLVLSQIAIQPLCIVRVKIIGVMQMIDQG
                     KPDDKLISVACHDKSVSHIEEIKGMPPIFDSELKQFFEEYKRLEQKTVLVEEFADRKK
                     ALDILEDALQRYKELYP"
     gene            complement(1285..2424)
                     /locus_tag="PLM68_08310"
     CDS             complement(1285..2424)
                     /locus_tag="PLM68_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOT55394.1"
                     /translation="MKKSMFTLFALCIVLNGNLLKAQDSLLAFIHNEALSPDEYIIEK
                     FHTNDVVLLGEHHLIKQNLLFVQDLIPKLYKHGIRNLGMEFGAQEVQDKLDSLVNAPE
                     YDQDLAQEIMFTYNCTWGYQEYVDIYKAAWRLNRSLPQDAPKFRILNLSYIFRWDKFT
                     PGPRNPENVAAVFTRGTVDKFRAEIIEQEVLQKGEKVLALVGTTHAFTKYGSPYFKYN
                     GDNFCDYDHDWLGGRLYRKYPGRVFNIMLHQAFNKREGDSYIQISPLEGLLEKIMALN
                     GNKPVGFDLLDSPMGRQPDPSIYSMCYKDFTLGQLFDGYIFLKPLSQLEGCTPIKGFV
                     NEQNIEEALRQFPDPDWHAPVKNLEDMVRFIDENPRSMIRGYNSL"
     gene            complement(2445..3089)
                     /locus_tag="PLM68_08315"
     CDS             complement(2445..3089)
                     /locus_tag="PLM68_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT55395.1"
                     /translation="MKRIRYILFDFDGTLADTFDLSFALYDRIAGEYGCEPLKQEDRQ
                     IIAAGRPQDLLRKYNMSAHKLGQITLRIRKEIHDHVPHMKPFEGMKEALTAIKDKGYR
                     LGIITSNARPNVLLFLENNGMDNLFDFVYSGRSLFGKDKVFKRMFAKKNIHPASAIYV
                     GDETRDIEACRKVGIPIVSVTWGMNNREILSSLHPDQMAHTPQEIIRCIENITL"
BASE COUNT          788 a          742 c          773 g          845 t
ORIGIN      
        1 tatccaggct tcctcgtctc caccggctcc atgcaacaag tacaggacag ggtatttgcc
       61 tcttgggttg ttctcgtaac cgtatggcgt ataaatggtc attctgcgct gcatgcctat
      121 ggtgggtgaa tcgtaccaga cggtcctcac attgcccctt tgggtggctt ccttgtaata
      181 tcccgagata ccaccaggga ccagcaatac gctcagataa cgcgttccgt cgcgctgcat
      241 gataacgttg gagggatcat tcatcattac cccgtccact atgaagctgt aggtgtaaat
      301 ttcgggggca ggcaggggga tgctggcata ccatacaccg gcagtatcgc gtgccatggc
      361 cagggaagaa cggtaatcgg ccgtccatga accgtacagg cggacttcat aagctttggg
      421 agccagcagg cggaaagtca cctggttttc agtaatttcg ggagagacaa tgggctgcct
      481 gccaaaagcg aaattgcgta gttcctgggc agctgccgtg ccggccagtg tggtcatcag
      541 aaggatcaga agagatattt ttttcataag atcgtatata atagtttcac atatgcaaat
      601 ttgacagtta taaaccggaa aagtttatgt atacgttcat caatattttc tatttgtgcg
      661 aaaagttata actttattcc acaaacccat atatcatgat ggtacataat ccctggcaca
      721 gtgttgaacc cgaggctaaa ggattcgagg tcaatgcgct catagagatc agcaacggaa
      781 gccgcgccaa gtacgaactg gacaaggaaa ccggactgtt aaagcttgac cgggtattgt
      841 attcggcagt ctactatccg gccaattacg gattcatccc ccgtacttat gccaaagatg
      901 atgacccgct ggacgtgctg gtactgtcac agatagccat tcagcccctg tgcatagtca
      961 gggtcaagat cattggcgtg atgcagatga ttgaccaggg caagcccgat gacaagctca
     1021 tttccgtggc atgtcacgac aagagcgtaa gccacattga agaaatcaag ggcatgcctc
     1081 ccatctttga ttccgaactc aagcaattct ttgaggagta caaacgcctg gaacaaaaga
     1141 cagtcctggt ggaagagttc gcagacagga aaaaagccct ggatatcctg gaggatgcgc
     1201 tgcaacggta taaggagctg tatccctgat tagcattcag atttctgaca caaccttaca
     1261 tgctaagaag gagtaggtca ggtcttaaag agaattgtaa cctcttatca tagaccgggg
     1321 gttttcatct atgaaccgga ccatgtcttc caggttttta accggagcat gccagtccgg
     1381 atccgggaac tggcgcaagg cttcttctat gttctgctcg ttgacaaaac cttttatggg
     1441 tgtgcatcct tccaattgag acagcggttt gaggaaaata tatccatcaa agagctgacc
     1501 cagggtgaaa tccttgtagc acatactgta tatgctggga tcgggctgcc gccccatggg
     1561 agaatccagc aggtcaaagc ccacaggctt gttgccgttc agcgccatga tcttttccag
     1621 taagccttca agaggggata tttgtatata actatcaccc tctcttttgt tgaaagcctg
     1681 gtgcaacatg atattgaaca cccttccggg gtacttcctg tacaatcgtc ctcccagcca
     1741 gtcatggtcg taatcacaaa aattgtcacc gttgtatttg aagtagggtg aaccgtattt
     1801 ggtaaaggca tgggtagtgc ctaccagggc aagtaccttt tcaccctttt gaagcacttc
     1861 ctgctcgatg atctctgccc tgaatttgtc tactgttccc ctggtgaaaa cagctgccac
     1921 attttccgga ttacgtgggc cgggggtgaa tttgtcccat ctgaaaatat agctcaggtt
     1981 caggatcctg aattttggag cgtcttgcgg aagcgaacgg ttcaacctcc aggctgcttt
     2041 gtagatatcg acatattcct ggtatcccca tgtacaattg taggtgaaca tgatctcctg
     2101 cgccagatcc tggtcgtatt caggagcatt gaccaggctg tcgagtttgt cttgaacttc
     2161 ctgggccccg aattccattc ccaggttccg gatgccgtgc ttatacagtt tggggatcag
     2221 gtcctgcaca aacagcaggt tttgttttat caggtgatgc tcacccagta gcactacatc
     2281 atttgtatgg aatttctcaa tgatgtattc atccggtgac aaagcctcat tatgaatgaa
     2341 tgctaagagg gagtcctggg cttttaacaa attgccgttc aggacaatac acaaggcaaa
     2401 tagcgtaaac attgactttt tcattacatt agggcattta tatgttataa cgtaatattc
     2461 tcgatacacc ggatgatctc ctgcggggta tgggccatct ggtccggatg cagggatgag
     2521 aggatttccc ggttgttcat gccccaggtg accgaaacaa tggggatgcc cactttccgg
     2581 caggcttcga tatcccgggt ttcatccccg acataaatgg ccgaggcggg atgaatgttc
     2641 tttttggcga acatcctttt gaacaccttg tcttttccaa aaaggctcct tccgctgtaa
     2701 acaaagtcaa acaggttgtc catgccgttg ttttcaagga acaagagcac gttgggccgt
     2761 gcattggaag taatgatacc cagccggtaa cccttatcct taatggccgt aagggcctct
     2821 ttcatgccct caaagggctt catgtgcggt acatggtcat ggatctcttt ccgtatacgg
     2881 agtgtgatct ggccgagttt gtgggctgac atgttgtatt tcctcagcaa atcctgcggt
     2941 cttccagcgg caatgatttg cctgtcttcc tgtttcaggg gctcacagcc gtattcgcct
     3001 gcaatgcggt cgtacagggc aaaggaaagg tcaaaggtat cggccagggt accgtcaaag
     3061 tcaaaaagaa tgtatcggat gcgtttcacg atgcttgttt aaatattaag ataaccattc
     3121 tcgtcataag cttcattcct tttctgct
//
LOCUS       DAOSOF010000181         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_31099_length_3131_cov_3.989272, whole genome shotgun sequence.
ACCESSION   DAOSOF010000181 DAOSOF010000000
VERSION     DAOSOF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31099_length_3131_cov_3.989272"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLM68_08320"
     CDS             complement(<1..396)
                     /locus_tag="PLM68_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOT55396.1"
                     /translation="MSLKKSAIRQLAGETAVYGMSTILARVLNFLLVPLYTRILTDQA
                     AYGVVAEFMAYIAVLQVVLVMGLETGSFRYASLAGQKNDKAGAARVFSTSLTTVTLLA
                     GLFFAVVVMLREPIAGKLGYAEFPQSIIYV"
     gene            complement(401..1141)
                     /gene="rlmB"
                     /locus_tag="PLM68_08325"
     CDS             complement(401..1141)
                     /gene="rlmB"
                     /locus_tag="PLM68_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT55397.1"
                     /translation="MSEDRIYGIHPVREALLGKRKPEKILLRQGMDSNTAAQMMDLAK
                     KFDVPVQFVPQEKLNHLTANKNHQGVVAMLPQVDYVTLDEAVAAAGTKPGFPTIILLD
                     GVSDVRNFGAIARTCECAGAGALILPAKGGAAVNQDAIKASAGALLRIPSCKVPNLRT
                     AVFHLRESGYVVIGTSAGQRQSIYETDFCRPLALVMGAEGKGISRGIMELCDELASIP
                     QFGEIGSLNVSAATAVVLFEAVRQRLKK"
     gene            complement(1134..3092)
                     /locus_tag="PLM68_08330"
     CDS             complement(1134..3092)
                     /locus_tag="PLM68_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT55398.1"
                     /translation="MISVSNICVSFAYRELFRDVSFTIDRRDRIALVGKNGAGKTTLI
                     RIIAGLQEPTSGRVVRSSDCRIGYLPQQMEHAKDKTVLEEALTVFFHLEELHRRMDNI
                     SAELSGREDYDSEAYKKLMVDLNEVSDRLRILSSDNPRGNAERILTGLGFKQDELDRP
                     SRTFSEGWNMRIELAKLLLSRPDILLLDEPTNHLDIESLRWLEQYLETFPGALLVISH
                     DRTFLDTVTTRTIEISLTRIYDYKAPYSQFLELRREYLEQQMAAYQNQQRTIEKTQEF
                     IERFRYKPTKSNQVQSRIKQLEKMELIEVDHLDNSRIRITFPEVSRSGKVVFNCRDVS
                     VGYDKGPIFTGANLVVERGEKVAFAGRNGEGKTTMLKLLTGQKSVMKGVFETGYNVQT
                     GYYAQNQDELLDNNLTVYQTLDGIAVGDVRTRLRDILASFLFRGEDVDKKAGVLSGGE
                     RSRLAMAKLMLQPYNVLLLDEPTNHMDIPSREVMKEALKQYNGTLVVVSHDRTFLDGL
                     VDKIYEFRDGKVKEHLGGIREFMERRKLETLKELEQADPAREEVRTENTGKESRAGEP
                     YRLKKERDKTLRKLQQQVTHWENRIEQHEKGIALMDEKMLNTPPGGFGTAFYQLYDKE
                     KRELEQAMKSWESAHDELDKFIETIQNE"
BASE COUNT          693 a          853 c          708 g          877 t
ORIGIN      
        1 tacataaata atggattgcg ggaattctgc atatcccagt ttcccggcga tcggttcccg
       61 taacatgacc accactgcaa aaaacaaacc ggccagcagc gttacggtag tgagcgaagt
      121 tgaaaacacc ctggcagctc cggccttgtc gtttttctgt cctgccagag aagcatagcg
      181 gaagctgccg gtctccagac ccatgaccag gaccacctgc aacacggcaa tgtatgccat
      241 gaattcggcc accactccgt aagctgcctg atctgtcagg atgcgggtat acaagggtac
      301 aagcaggaaa ttcagaaccc ttgcaaggat cgtgctcatc ccgtatacgg cagtctctcc
      361 tgccaattgc cttatagctg atttttttaa agacattgtc ttactttttc aaacgttgtc
      421 tgaccgcctc gaaaagcacg actgctgttg cagccgatac gttcaatgag ccgatttccc
      481 caaattgggg tatggaagcc aattcatcgc acagttccat gatcccgcgc gatatgcctt
      541 ttccttcggc acccatcacc agcgccagcg gacggcaaaa atccgtttca tagatggact
      601 gacgctgtcc ggccgatgtt cctatgacca cgtaacccga ttccctgagg tgaaaaacag
      661 ccgtcctcag gttggggacc ttgcatgagg gaatgcgcag cagtgctccc gcagaagcct
      721 ttatggcatc ctggttcacg gcagcccctc ctttagctgg aagtatcagc gcacctgcac
      781 cggcgcattc gcaagtacgg gcaatggctc cgaaattgcg gacatcactt accccgtcca
      841 gcaggattat ggttggaaaa cccggtttgg tccctgcagc ggccacggcc tcatccagtg
      901 ttacataatc aacctgcggc agcatggcaa ccaccccctg gtggttcttg tttgccgtca
      961 gatgattcag tttctcctgt ggtacgaact ggaccggcac gtcaaatttt ttggccagat
     1021 ccatcatttg tgctgccgta ttactgtcca taccctggcg gagcaggatt ttttcaggtt
     1081 tcctttttcc caggagtgct tccctgacag gatgtatgcc gtaaatgcga tcttcactca
     1141 ttttgtatgg tttctatgaa tttatccagt tcatcatgtg ctgattccca gcttttcatg
     1201 gcctgttcca gctctctttt ttctttgtcg tacaactggt aaaacgctgt gccgaatcca
     1261 ccgggaggcg tgttcagcat tttttcgtcc atcagggcaa tccctttttc gtgctgttca
     1321 atcctgtttt cccaatgcgt gacttgttgc tgcagcttgc gcaatgtttt atcccgttcc
     1381 tttttcaggc ggtaaggctc ccctgccctt gattctttac cggtattttc agttctaacc
     1441 tcttccctgg caggatcggc ttgttccaat tctttgagcg tttcgagttt cctgcgttcc
     1501 atgaactcgc gaatcccgcc caggtgctct tttacctttc catccctgaa ttcgtatatt
     1561 ttatcaacaa ggccgtccag gaatgtcctg tcgtgagaaa ccaccaccag ggtcccgttg
     1621 tactgtttaa gggcttcttt catcacttcc ctggaaggaa tatccatgtg attggtgggt
     1681 tcgtccagca acagaacatt atagggctgc agcataagct tagccatggc caggcgcgat
     1741 ctttccccac cgctcagcac tcctgctttc ttatccacgt cttccccgcg aaagaggaat
     1801 gaggccagga tatcacgaag tcgtgtacgg acgtctccca ccgctattcc atccaatgtc
     1861 tggtatacag tcaggttatt atccagcaat tcgtcctggt tttgtgcata atagcccgtc
     1921 tgtacattgt accctgtttc aaaaacccct ttcatgacac tcttctgccc ggtaagcaat
     1981 ttcagcatgg ttgttttgcc ttctccgtta cggccagcaa atgcaacctt ttcaccacgt
     2041 tccaccacta ggtttgcccc ggtaaagata ggccctttat cgtaaccaac agatacatcc
     2101 cggcaattga acacaacttt cccgcttctg ctgacttcag gaaaagtgat tcgtatccgg
     2161 gagttatcca aatgatccac ctctatcagt tccatctttt ccagttgttt tatacggctt
     2221 tgcacctggt tggacttggt gggcttatag cggaaacgct caatgaattc ctgtgttttt
     2281 tctatggtac gttgctggtt ctggtatgca gccatctgtt gttccaggta ttccctgcgt
     2341 aattccagga attgggaata cggggcttta taatcgtaaa tcctggtgag agaaatttca
     2401 atggtcctgg tagtcaccgt atccaggaag gtcctgtcat gcgatatcac cagcagggcc
     2461 ccgggaaaag tttccaggta ctgttccagc cacctgaggc tttcaatatc cagatggttg
     2521 gtgggttcat ccaataacaa tatgtcaggc cgggaaagca acaattttgc cagttctatg
     2581 cgcatattcc acccttcgga aaaagtccgg ctggggcggt ccagttcgtc ttgtttgaag
     2641 ccaagaccgg tcaggattct ctcggcgttc cccctgggat tgtctgaaga caggatacga
     2701 agccggtcac taacctcatt caggtcaacc attaatttct tgtaagcttc agaatcataa
     2761 tcctctcttc ccgaaagctc tgcggagata ttatccatgc gccggtgtag ttcttccagg
     2821 tggaaaaaaa ccgtaagcgc ttcttccagc acggttttgt cctttgcatg ttccatttgc
     2881 tggggaagat atcctatccg gcagtcggaa gaacgtacca ccctgccaga cgttggttcc
     2941 tgcagacctg ctatgatcct gatcaatgtg gtttttccgg ccccgttctt tcccaccagg
     3001 gcgatcctgt cgcgccggtc aatggtaaaa gaaacatccc ggaaaagctc cctgtaggca
     3061 aaagaaacgc agatattgga aacagatatc attccatttt tctccccagc atgtcaatgg
     3121 taatgagctg a
//
LOCUS       DAOSOF010000182         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_31658_length_3088_cov_5.912628, whole genome shotgun sequence.
ACCESSION   DAOSOF010000182 DAOSOF010000000
VERSION     DAOSOF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31658_length_3088_cov_5.912628"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLM68_08335"
     CDS             complement(<1..998)
                     /locus_tag="PLM68_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT55399.1"
                     /translation="MAFPDKAYVSLENPDTRRFAIEDPRGFIATYPNGAILDEIQRTP
                     DIFSYLQQVLDENQGKGRFILTGSNNFLLQENISQSLAGRVGYLYLLPLAINELPGDT
                     PGELPGKRLSANELMHKGFYPSLYREEEQIDVGKYYTNYIRTYTERDVRMIRNVTDLY
                     TFERFLRLCAGRIGQLLNMNNLAVETGVDNKTIGAWLSVLEASFVVFRLQPYHKNFNK
                     RIVKMPKLYFYDTGVASALLGIENAAQMALHPFRGSLFENMVIVNFLKSRYNRGKTNN
                     LYFWRDSLGTEVDLIVEESDTLLPIVIKSGQTITEDYFKSISSWLKISGMKKGWVI"
     gene            complement(1465..2955)
                     /locus_tag="PLM68_08340"
     CDS             complement(1465..2955)
                     /locus_tag="PLM68_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOT55400.1"
                     /translation="MDTEMKEYIDFTLKDWLYQAHIMEVIEHKTFLEDGPIVLKRIEN
                     EDYMQIPLFRQVAYLCETVREAKTLKLTTTGNLPRAVVHGIYKLGILDHYFEENIARL
                     RTENDWYTVPLTRLLAEMGGLIKKRSNALMLTKQGEKVLKDRHLLLKSILITFGHKLS
                     WAYFDLFEDRSLGQQNFGLSLLLMADYGNQPRDARFYSERYFYHNSHGSSSRYAQHCY
                     NTRTLDRFMQNLGLITYDKTHRFGEQEQVTVAKTPLFDKLLAIDRNFGKISYRAETKA
                     EALAVSQAVPDAVHKPEAQVQAQAVAQAVVPLYRLHISLNGAQPAIWREFVVPSDISL
                     VNFHVVIQAVMGWTNSHLHLFRKKGVEYTYRYRSDNYWDEEGYVDYAGMKIADLMTDC
                     GDSVTYLYDYGDDWKHTIELLEVIKNIKVRTGTFFLQCLAGERRCPPEDCGGVHGYHE
                     MLKTLKNPAHSEYKDTVTWLGDDFDPNYFNLLEVNNILASYCKECV"
BASE COUNT          803 a          742 c          637 g          906 t
ORIGIN      
        1 taaattaccc atcctttttt catgccagat atctttagcc aggaggagat ggatttaaag
       61 taatcttcag ttatggtttg tcccgatttt attactatcg gcaacaaagt gtcagattct
      121 tctactatca aatcaacttc tgtacccagg ctgtcccgcc agaaataaag attattggtt
      181 ttcccccggt tataccggct ttttaaaaaa ttaacgataa ccatattttc aaacaaactg
      241 ccgcgaaaag gatgtaaggc catctgtgct gcattctcaa tccccagcaa tgcagatgcc
      301 accccggtat cgtaaaaata cagcttcggc atttttacta tacgcttatt aaagttctta
      361 tgatacggct gcagacgaaa aaccacaaag ctggcctcca ataccgataa ccacgctcct
      421 atggttttat tgtcaactcc ggtttctaca gccagattat tcatgttgag cagctgacca
      481 atacgcccgg cacacaatcg aagaaatctt tcaaaagtat ataaatcggt aacgtttcgt
      541 atcatcctga cgtcccgttc tgtgtatgtg cgtatgtaat ttgtataata cttacctaca
      601 tcaatttgtt cttcttcccg ataaagagat ggataaaatc ctttgtgcat aagctcattg
      661 gccgataacc tcttacctgg tagctcacct ggcgtatcac ctggtaactc gtttattgcc
      721 agaggcagca gatacaggta acccaccctt ccggccagac tttgagatat gttctcctgg
      781 agaaggaaat tgtttgatcc ggtaaggata aaccggcctt taccctggtt ttcatccaac
      841 acctgctgaa gatatgaaaa tatgtctggt gtgcgttgta tctcgtccag aatggcacca
      901 ttgggataag tggcaataaa accacgcggg tcttctatgg caaaacgcct ggtatcagga
      961 ttttccagac taacatacgc tttgtcagga aatgccatgc gtaccagcgt ggttttgcct
     1021 gattgccggg ggcctataac cgccacactt ttaaactgcc cggctaatga ctttaattct
     1081 ttttctgcaa tacgagttat cagcataatg caaatataca attttggatt tcaattccaa
     1141 aattgtgatt gagtgctgca aagcagcatt ataataagcg ccaaagaact ttggcgcacc
     1201 gccttggggt gccgcgcatt cttgatgcat cacgttgact tttagcatta taacaaaaca
     1261 gctccctggg ggagctgttt tgttaacgat ctgttatata ggcaaatgca tcaagagtac
     1321 gcggcactca accgccccgc tcttactcgt ggtcaaagac taccaggctg tcctttatca
     1381 acgtagcatg tccctgccat ggtgtcctgt tttaggaata tggtatcgcc catgaaatat
     1441 aacacagcat ctttatattt tgtttcacac acattcttta cagtaacttg ctagtatgtt
     1501 gttcacttcc aggaggttaa aatagttggg atcaaaatca tctcccagcc aggtcaccgt
     1561 atctttatat tcactgtggg cggggttttt caatgtcttc aacatttcat ggtagccatg
     1621 aacccctcca caatcttctg gcgggcatcg tctctctccg gcaaggcatt gaagaaaaaa
     1681 agtcccggtc cggaccttta tgtttttgat gacctccaac aattctatag tatgtttcca
     1741 gtcgtcaccg tagtcgtaaa gataggtaac ggaatcgcca cagtcagtca ttaaatcagc
     1801 gattttcatt cccgcatagt ctacatagcc ttcttcgtcc caataattat ctgaccggta
     1861 tctgtatgtg tactcgactc cttttttccg gaacaggtgc aaatggctgt tggtccagcc
     1921 catcaccgcc tgtatcacca catgaaagtt tacgagcgaa atgtccgagg ggaccacaaa
     1981 ttcccgccag atggccggtt gtgcgccgtt tagcgatatg tgcaaccggt aaagcggcac
     2041 aacagcctgg gccacagcct gggcttgaac ttgagcttca ggtttgtgca cagcgtcggg
     2101 tacagcctgg gacacagcca aagcctcggc cttggtctcg gcccgataac tgatttttcc
     2161 aaaattgcgg tcaatggcta aaagtttgtc aaagaggggt gtttttgcga cggttacctg
     2221 ctcttgttct ccaaaccgat gcgtcttatc gtaagtgatc agtcccaggt tttgcataaa
     2281 ccggtcaagc gttctggtat tgtaacaatg ctgtgcgtac ctgctactgc ttccatggga
     2341 gttgtggtag aagtaccttt ccgagtagaa gcgggcgtct ctgggctggt tgccgtagtc
     2401 cgccatcaaa agcagcgaaa ggccaaaatt ctgttgcccc agcgaacgat cctcaaataa
     2461 gtcaaaatac gcccaactga gtttgtgccc aaaagtgatc aggattgatt tcagtaacaa
     2521 atgcctgtct ttcagcacct tttcaccctg tttggtgagc attaaggcat tgctgcgttt
     2581 ttttatgagc ccacccattt ctgccaggag cctggtcaga ggcaccgtgt accaatcatt
     2641 ttcagtccgt aaccgcgcga tattctcctc aaaataatga tccaaaatgc cgagtttata
     2701 aatcccgtgt accacggctc ttgggaggtt gccggtagtt gtgagtttga gggttttcgc
     2761 ttcccggacg gtttcgcaca ggtatgcaac ctgcctgaac agaggaatct gcatataatc
     2821 ctcattttca atacgtttca aaacaattgg cccgtcttcc aaaaaggtct tatgctcaat
     2881 cacttccatg atatgcgctt gatacagcca atctttcaac gtgaaatcta tgtattcttt
     2941 catttctgtg tccataagct cttaatctct aaattttttt aattccaggg caacggccaa
     3001 aaaccaagta tccctgagag ttccttgtct tcatatatat ctttaagaat ctgaaagaga
     3061 tatagcttat gcttgtttat atccagca
//
LOCUS       DAOSOF010000183         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_31950_length_3065_cov_4.020930, whole genome shotgun sequence.
ACCESSION   DAOSOF010000183 DAOSOF010000000
VERSION     DAOSOF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31950_length_3065_cov_4.020930"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLM68_08345"
     CDS             complement(<1..295)
                     /locus_tag="PLM68_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55401.1"
                     /translation="MKLRFKALFICVLLLFTGTVAGQTADSLTVKTTDTLCTAIPDSL
                     TKAYLDRLSLPYAEAGRVPAVDSLLRVIRDEKGLFYELDSLGLMILNEAGIPVI"
     gene            complement(323..1309)
                     /locus_tag="PLM68_08350"
     CDS             complement(323..1309)
                     /locus_tag="PLM68_08350"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOT55402.1"
                     /translation="MKRFSLFHNLALVMIAVTIGSCAQGNKEKERKPLTFSQPPIPVL
                     LTSGSDILGYRILHYWDLFPFKDSLCIQQENFAEQAFTGFVNLLQEDPHVTARDAIER
                     MMTTAGTAADDPVVRKKILLKFFSLSEYFFYHPNSPYRNDDHFQAVLESMVASPLLDT
                     VEKQRPVYLLEMIMKNRVGEMARDIRFVRAVRNFPNTDDPASPETLYNLKSDYILVFF
                     INLGCNACRQAADEILASELLTEMVQQKRLVILTIYPDKDLEGWQKYLPTLPSGWIHG
                     YDPEGELRNGRSYDLKAIPSLYLLDGSKRVLVKDALSVSQIEDTIRQEAHFL"
     gene            1353..2120
                     /locus_tag="PLM68_08355"
     CDS             1353..2120
                     /locus_tag="PLM68_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOT55403.1"
                     /translation="MTPDTFRIILYVMAALAVVVFIALYQVKAGYGILIDKSWGITIS
                     NKWAWMIMEAPVFIAMCWFWVMSPRKWDLVPFIFFLLFQIHYLQRSFIFPWLIKGKSR
                     MPVLIMLMGIVFNLANAFIQGQWIFFLAPETMYTNSWLRDPRFLLGIILFLTGLIVNI
                     QSDNTIRHLRKAGDNNHYLPERGLFRYVTSANYLGEIVEWLGFAVLTWSLSGFVFFLW
                     TFANLVPRAHAIHKRYQEEFSSEMASEPRKRIFPFIY"
     gene            2127..>3065
                     /locus_tag="PLM68_08360"
     CDS             2127..>3065
                     /locus_tag="PLM68_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT55404.1"
                     /translation="MLRNSLLFTPASIGPLTLRNRTIRAAAFEGMCIDNGPSQALFNY
                     HTAVAAGGVGMTTVAYAAIARSGLSFPTQLWMRPDIVPGLRHLTDGIHKEGAAASIQM
                     GHCGNMSHRKICGCMPVSASSGFNMYSPTFVRAMKKTEILQMVKDFGKATELARESGF
                     DAVEIHAGHGYLLSQFMSQWLNRRKDEYGGSFENRLRFTSQAIEEVMEVATRHNIAVL
                     VKMNMRDGFRGGMELDESLEVARLLEKLGVNALVLSGGYVSRAPMYVMRGAMPIRTLT
                     YYMPWNYVKVGVRLVGRLMIPSVPFKEGYFLEDAREF"
BASE COUNT          769 a          722 c          784 g          790 t
ORIGIN      
        1 ctataaccgg gatacccgcc tcattgagaa tcatcagtcc caggctgtcc agttcgtaaa
       61 acaagccttt ctcatccctg atcaccctta acagggaatc aacggcagga acccgtcctg
      121 cctctgcata aggcagtgaa agcctgtcca aataggcctt tgtcagcgaa tcgggtattg
      181 cggtacacag cgtgtctgtc gttttaacgg taagggaatc agctgtctgg cccgcaactg
      241 tccctgtgaa aagcagcaaa acgcaaataa acagtgcttt aaaccgtaat ttcatgtatt
      301 ctcagtatga cgaggcgcaa agttacaaaa aatgtgcctc ttgtctgatg gtatcctcaa
      361 tttgcgacac agagagggca tctttgacga gaacgcgttt tgaaccatcc agcaggtaca
      421 gcgatgggat ggctttgagg tcataggagc gcccgttccg taattcccct tcaggatcat
      481 agccgtggat ccacccggaa ggaagagtgg gcaggtattt ctgccagcct tccaggtctt
      541 tatcggggta tatggtcaga attaccagtc ttttctgctg taccatttct gtcagtaact
      601 ctgaagccag gatttcgtct gcagcctggc ggcatgcatt gcatcccagg ttgatgaaga
      661 agaccagtat gtaatctgac ttcaggttgt aaagtgtctc cggcgatgcc ggatcatccg
      721 tattaggaaa attcctgaca gcacgtacaa aacgaatgtc ccgtgccatt tcacccaccc
      781 ggtttttcat gatcatttcc aagagataaa caggccgctg tttctccacg gtgtccagca
      841 gggggcttgc aaccatgctt tccaggacgg cctggaaatg atcatcgttc ctgtagggtg
      901 aattggggtg gtagaaaaag tattcggata aggagaagaa cttaagcagg atttttttcc
      961 tgacaaccgg atcgtctgct gcagtgccgg ccgtggtcat catcctttca atggcatccc
     1021 gggcggtgac atggggatct tcctgcaaca ggtttacgaa accggtaaag gcctgttcgg
     1081 cgaaattttc ctgttggatg cagagtgaat ccttgaaggg aaacagatcc cagtaatgaa
     1141 gtatccggta cccgaggatg tcagaaccgg aagtcaggag aaccggtata ggtggttgtg
     1201 aaaaggttag gggtttccgt tctttttctt tgtttccctg tgcacaggac cctatggtta
     1261 cagcgatcat gaccagggcc aggttatgaa aaagggaaaa acgcttcata aaacaaaaat
     1321 acaaaaaaat gttacatttg tccgttatga atattacacc tgacactttc cggatcatac
     1381 tgtatgtgat ggcagccctg gcagtagttg tatttattgc cctttaccag gttaaagccg
     1441 gctatggaat tctgatagac aaaagctggg gtatcaccat atccaacaag tgggcctgga
     1501 tgatcatgga agcgcccgta tttattgcca tgtgctggtt ttgggtgatg tcaccccgca
     1561 aatgggattt ggtgccattc atctttttcc tgctgttcca gatccattac ctgcagcgtt
     1621 cctttatctt tccctggctg atcaagggaa aaagcaggat gccggtgctt atcatgctca
     1681 tgggtatcgt tttcaacctg gccaatgcct ttattcaggg tcaatggatt ttcttcctgg
     1741 cgcctgagac aatgtatacc aattcctggc tcagggatcc aaggtttctc ctgggaatca
     1801 tcctgttcct gacaggactg atagttaaca tacaatccga caacaccatc cggcatttac
     1861 ggaaagcagg ggacaacaac cattacctgc ccgaaagggg cttgttcaga tatgtcacca
     1921 gtgccaacta cctgggagaa atcgttgaat ggcttggctt tgctgtcctt acctggtcat
     1981 tgagcgggtt tgtctttttc ctgtggactt ttgccaacct ggttcccagg gcacatgcca
     2041 tccacaaaag ataccaggag gagttctcct cagagatggc cagtgaaccc cgaaaacgaa
     2101 tatttccttt tatttattag atcaacatgt taaggaattc cctcctgttc acccctgcct
     2161 ccataggtcc cctaaccttg cgcaacagaa ccatacgggc tgccgctttt gaaggaatgt
     2221 gcatcgataa tggtccctca caggcattgt tcaattacca cactgccgtt gcggcaggtg
     2281 gtgtaggcat gacaacggtg gcttacgccg ccatagccag aagcggattg tcgtttccta
     2341 cccagttatg gatgaggccg gatattgtcc ccggtctgcg tcacctcacc gacgggatac
     2401 acaaagaagg agcagctgct tcaattcaga tggggcattg cgggaacatg agccaccgca
     2461 aaatatgtgg atgcatgccg gtatcggcaa gcagcggttt caacatgtat tctcccacat
     2521 ttgtccgtgc catgaaaaaa accgagatac tgcaaatggt aaaggatttc ggaaaagcta
     2581 cagagctggc aagggaatca ggctttgacg ccgtagagat acacgccggg cacggctacc
     2641 tgctaagcca gttcatgtct cagtggctaa acaggagaaa ggatgagtac ggaggtagtt
     2701 ttgagaaccg tctcaggttt acttcccagg ccattgagga agtgatggag gtggcaaccc
     2761 gccataatat agccgtgctg gtcaagatga acatgaggga tggattcagg ggaggtatgg
     2821 aacttgacga gtctcttgag gtggcaagac ttctggaaaa actgggcgtt aatgccctgg
     2881 tgttgagtgg cggttacgtg agcagggccc ccatgtatgt gatgcgaggt gccatgccca
     2941 tcaggacact gacttattat atgccctgga attatgtaaa agtgggtgtc cgtctggtcg
     3001 ggcgcctgat gataccttct gttcccttca aggaaggtta cttccttgaa gatgcacgtg
     3061 aattc
//
LOCUS       DAOSOF010000184         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_32407_length_3034_cov_4.810003, whole genome shotgun sequence.
ACCESSION   DAOSOF010000184 DAOSOF010000000
VERSION     DAOSOF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32407_length_3034_cov_4.810003"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="pgk"
                     /locus_tag="PLM68_08365"
     CDS             <1..408
                     /gene="pgk"
                     /locus_tag="PLM68_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT55405.1"
                     /translation="DVVAASAFENDARTVICPIDKIPDGWFGMDTGPEALQQWKEVIL
                     GSKTILWNGPAGVFEMPSFARGTLQLVEIVAEATHNGSFSLVGGGDSVAAVTRMGYAD
                     KVSYVSTGGGAMLEYLEGKELPGIQAIGAIRPS"
     gene            complement(395..955)
                     /gene="frr"
                     /locus_tag="PLM68_08370"
     CDS             complement(395..955)
                     /gene="frr"
                     /locus_tag="PLM68_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT55406.1"
                     /translation="MSDAKKVLEQTQGKMNKAVEFLENELATYRVGKANPAVFSGVHI
                     DYYGTSTPLSQVSSISVPDARTMIIQPWDKSLIPAIEKAIMAANLGFTPQNNGEIIRI
                     MVPPLTEERRRELVRKARTEGENARISIRNARRDGVDVLKKYQKDGLSEDVVKDTEIL
                     IQKEVDVFSKNVEEVLASKEKEIMTV"
     gene            complement(970..1674)
                     /gene="pyrH"
                     /locus_tag="PLM68_08375"
     CDS             complement(970..1674)
                     /gene="pyrH"
                     /locus_tag="PLM68_08375"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT55407.1"
                     /translation="MKYSSILLKLSGESLGGKEGQGLDSRVLENYAREIKPLADAGIR
                     IAIVVGGGNIFRGLKGAMNGYDRVHGDQMGMLATTINSIALADALQAQGMKAMVYTAT
                     PMRPIARYYHRDEVLRKMEKRTIVLLSGGTGNPFFTTDSAAALRAVELGVDALLKGTR
                     VDGVYSDDPEKVPDATRYDTLTFDQVLQKNLGVMDMTAFTLCRENNMPIVVFNMNGRG
                     NLEKVVKGEKTGTIVK"
     gene            1748..2395
                     /locus_tag="PLM68_08380"
     CDS             1748..2395
                     /locus_tag="PLM68_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOT55408.1"
                     /translation="MASSITKKAIMSVTGLFLILFLTLHVTANLTILWGREAYDTVTE
                     FMGTNTFIQLVVPLLALGFVIHIGYSLILNLKNLKARGPVGYAGGMKSRSAGFDRRAS
                     QNMLVLGIIVLGFLVLHLSQFWIKMQWQHLTGGDPENGYLLVTGYLGTPWVAICYIAW
                     FAALWFHISHGFWSAFQTLGLSNQRILPVLRIVSMIYSSLLFAGFTVIVIWCMFF"
     gene            complement(2415..2906)
                     /locus_tag="PLM68_08385"
     CDS             complement(2415..2906)
                     /locus_tag="PLM68_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55409.1"
                     /translation="MKGFNPWIYLRRVAKYIVFYAIIILLMLLIVYYTSDNRSSPFWE
                     AMIPSDNRTKLIILIVAFSTIYPFLGFSRKEVYTNRFSEEDKEAATRIVSESGFILIS
                     DQDGKMVFKSKRITMRIFRVFEDKITLDYRDNPLTIEGMRRDIQRIASHLEHYFRGVS
                     DDQ"
BASE COUNT          676 a          769 c          756 g          833 t
ORIGIN      
        1 gatgtggtgg ctgcctcggc tttcgagaac gacgcccgga ctgtcatatg ccccatagac
       61 aagatcccgg acggatggtt tggcatggat acaggtcccg aagccctgca acaatggaaa
      121 gaagtcatcc tcggctccaa aaccattctc tggaacgggc ctgccggcgt ttttgagatg
      181 ccttcctttg ccagggggac attacaactg gtggagatag tggcagaggc cacgcacaac
      241 ggctccttct cgctggtagg cggaggagac tcggtggctg ccgtaacccg gatgggatat
      301 gcagataagg tcagttatgt ttcaacagga ggcggcgcca tgctggaata cctggagggc
      361 aaagaactgc ccgggatcca ggccataggc gccatcagac cgtcataatc tctttttcct
      421 tggaggccag gacttcttcc acatttttgg aaaaaacgtc cacttccttt tgtatcagaa
      481 tttcggtgtc tttgactacg tcttcagaca atccgtcttt ctgatatttt tttagcacat
      541 ccacaccgtc ccgtcgggca ttgcggatgc tgatacgggc attctcccct tcggtcctgg
      601 ccttcctgac cagttcgcgg cgcctttctt cggtcagcgg aggaaccatg atgcggatga
      661 tctcaccgtt gttttgcggt gtgaacccca ggtttgccgc catgatggct ttttcgatgg
      721 cagggatgag gcttttatcc catggctgga tgatcatggt ccgggcgtcc ggaacagaga
      781 tactggacac ctgggacaag ggtgtggagg tcccgtagta atcgatatgc actccggaaa
      841 aaacagcggg attggccttc cccacacggt atgtggccag ctcattctca aggaactcca
      901 cagctttatt catcttgccc tgcgtttgtt ccaatacttt ttttgcatcg cttatttcca
      961 taatgtatct tattttacaa tcgttcctgt tttttctcct tttacgacct tctcaaggtt
     1021 ccccctgccg ttcatgttga acaccacaat gggcatgttg ttttcccgac aaagcgtaaa
     1081 ggctgtcata tccatgaccc ccaggttctt ctgcaatacc tggtcaaaag tcagggtatc
     1141 atacctggtg gcatcgggga ccttttccgg gtcatcgctg tagacaccgt ccacacgggt
     1201 tcccttgagc aaggcatcca ctcccaattc cacggcacgc agggccgcgg cagaatctgt
     1261 ggtaaagaat gggttccccg ttccgccgga aagcaggaca atggttctct tttccatctt
     1321 gcgcaatacc tcgtcgcggt gatagtaccg ggctatgggc ctcatgggag tggcggtata
     1381 gaccatggcc ttcatcccct gtgcctgcag tgcgtctgcc agggcaatgc tgttaatggt
     1441 ggtggccagc atgcccatct ggtctccgtg aacacggtcg tatccgttca tggcaccttt
     1501 caatccccgg aaaatgttac cgccgccaac tacaatggca atgcgtattc ctgcgtctgc
     1561 caggggtttt atctcgcggg catagttttc cagcacccgg ctatccaggc cctgtccttc
     1621 tttgcctccc agactttccc cgcttaattt cagtaatatg gaagaatact tcatgttttt
     1681 gtctttcata tcctacaaag atatataaaa aatccgtgta tctttgctct gctcacacag
     1741 atacatcatg gcatcctcca ttacaaaaaa agcgatcatg agcgtaacgg ggctttttct
     1801 gatcctgttc ttaacccttc atgtgacagc caacctcacc atattgtggg gccgtgaagc
     1861 ttacgacacc gtgacggaat tcatgggcac caatactttc atacaacttg tggttccgtt
     1921 gctggccctt ggttttgtga tacacatagg atattccctc atcctgaacc tgaagaacct
     1981 gaaagcccgc ggaccggtag ggtatgccgg gggcatgaaa tcccggagtg cgggctttga
     2041 caggcgggcc tcgcagaata tgctggttct gggaatcata gtcctgggat ttcttgtgtt
     2101 gcacctgtcc cagttctgga taaaaatgca atggcaacat ctgaccgggg gagatccgga
     2161 gaacggatac ctgctggtga cggggtatct gggtactccc tgggtggcca tttgctatat
     2221 cgcctggttt gcggccctgt ggttccacat cagtcatggc ttctggtctg cctttcagac
     2281 cctgggactg agcaaccaga ggatccttcc cgttctgcgc attgtgtcaa tgatttactc
     2341 ttccctgctg ttcgcgggat ttacggtcat tgtgatctgg tgcatgtttt tctaggcagg
     2401 atgcgttccc ggggctactg gtcgtcgctg acaccgcgga aatagtgttc caggtgactg
     2461 gcaatgcgtt gaatgtcccg gcgcatgcct tctattgtca gcgggttgtc ccggtaatcc
     2521 agggtgattt tatcttcaaa aacacgaaag atccgcatgg taatccgttt ggatttgaaa
     2581 accatttttc cgtcctgatc agaaatgagg ataaatcccg attctgagac aatgcgtgtg
     2641 gcagcttctt tgtcttcttc tgaaaaccgg ttggtataga cctctttcct ggagaaacct
     2701 aagaaaggat agatggtaga aaaagccaca atcagaatga tcagcttggt acggttatcg
     2761 gaaggtatca ttgcctccca gaatggggag ctgcgattgt cgcttgtata atagacaata
     2821 agcagcatga gcaatatgat gatggcataa aaaacgatgt attttgcaac gcggcgtaaa
     2881 tagatccacg ggttaaatcc tttcataagc actccgattt taaggtgtaa acaaatgtag
     2941 gtcttttttt tattactttt gtgataacta aactattcga ctatggaaaa aaatctcaaa
     3001 accattatca ttatcctgtc cgtagtagcc gcac
//
LOCUS       DAOSOF010000185         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_32627_length_3017_cov_3.621877, whole genome shotgun sequence.
ACCESSION   DAOSOF010000185 DAOSOF010000000
VERSION     DAOSOF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32627_length_3017_cov_3.621877"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2918)
                     /locus_tag="PLM68_08390"
     CDS             complement(<1..2918)
                     /locus_tag="PLM68_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55410.1"
                     /translation="MVIYFSVPRIGGTDISAVIGETSVYLSLIEVFDFLRIKNSYTAG
                     FESVNGFFITEDAAYNIDRVNNRITFKDKEFQLKAGDIIRTETNLFLKTELFGQVFGL
                     DCSFSYRSLSVSMNTSIELPVIREMRLAQMRKSLGQITGDIVADTVIGRKFPLIHFGM
                     ADWSAILTQEIKGPKGARLSLNLGGVILGGETNINLNYTTNTPFQERNQFYQWRYANN
                     KYKALRQVILGKINSFSTSTINSPVLGIQITNTPTTYRQSFGTYTLSDVTEPGWTVEL
                     YVNNVLVDYKQADASGFYTFEVPMIYGNTNVKLQFYGPWGEERSKEQTLNIPYNFLPA
                     GNFEYRLSAGMVSDSLWSKFSRLDMDYGITRFMTIGAGVEYLSSVTSGQIMPFVHTSL
                     RLAPGLLLTGEYTHGVNVKGILNWRFRSDIQLEVNYTRYDKDQKAINTNYLEERRIAM
                     SIPIRFKSFSVFTRMSLNQVIMPTTQYTTAELLLSGSLFGINTNLTTFATFSSASKPN
                     IYSTLSMSIKLPAAFILTPQTQFNYTQGKFVSVRAALEKRMFNNGYLNVSFERNFLNS
                     ITNLELGLRYDFSFAQIGFSARRSNNTYSFVETASGSIMTDPRSRLVAVNNRSTVGKG
                     GMILYPFLDMNWNGKRDKGEPKAFGMEVQVSGGRIQYIEKDTITRIYDLQPYAQYLVT
                     LNGSKFDNISWRMNNQTMSVIVDPNQFKRIEVPIVVVAEASGQVYLRDRNTLRGQGRI
                     TVQFYKNDTVILNSTLSESDGYFSYMGLAPGNYLVAVDPEQMRKLDMTATPAFIPFTV
                     EPSIDGDYINDLEFVIQSNAPATKPAAAPAQAAPPVTPTAATPAQAAQPMTPPAKKDT
                     LTSDPAKKISPSQAVTPAPVPVTPPLPPAEQKSKTGDTPIKQMTTPQVAESPVKSGYR
                     LQFLVTDKPLESGSNYLTHLKQRLPILEITAVLEEDGRYHYVSQYFRTRREALHWMRM
                     V"
BASE COUNT          721 a          675 c          771 g          850 t
ORIGIN      
        1 accatgcgca tccagtgcag ggcttcccta cgggtgcgga agtattggga tacataatga
       61 taacgtccgt cttcttccag aacagcagtg atttccaata ttggcagcct ttgtttcagg
      121 tgtgttaagt agttgcttcc actttccagg ggtttgtctg taaccaggaa ctgtaaacgg
      181 taaccgctct tgacggggct ttcggcaacc tggggagtag tcatctgctt tatgggagta
      241 tcacctgttt tacttttttg ttcagcaggc gggagcggag gcgtaaccgg tacgggagca
      301 ggagtgacag cctgggatgg ggatatcttt ttagcaggat cggatgttaa tgtgtccttt
      361 ttggcgggcg gagtcatagg ttgagccgcc tgtgcaggag tagcagcagt cggagttaca
      421 ggaggagctg cctgtgcagg agcagcagca ggctttgtgg caggggcatt tgactggatc
      481 acaaattcca ggtcgtttat gtagtctccg tcaatgcttg gctcaactgt gaagggaata
      541 aaggcgggtg tggctgtcat atccagtttt ctcatctgtt ccgggtctac tgctaccagg
      601 taattgcccg gggcgagccc catatagctg aaatagccat cagattcgga aagcgtgctg
      661 tttaaaatta ccgtgtcgtt cttgtagaac tgtacggtta tccttccctg gccacgcagg
      721 gtgtttctgt cacgaaggta tacctggccg gatgcttctg ccacgaccac tatggggacc
      781 tcaatccgtt taaactggtt gggatctact atcacactca tggtctggtt gttcattctc
      841 caggaaatgt tatcgaattt gcttccgttc agggtgacca ggtactgggc atagggctgc
      901 aggtcataga tacgcgtaat ggtatccttc tctatgtact ggatccgtcc cccgctcacc
      961 tggacctcca ttccaaaggc tttgggctct cccttgtccc gttttccatt ccagttcatg
     1021 tccaggaaag gatacaggat cataccaccc ttacctacag tgcttctgtt attaactgca
     1081 actaacctgg agcgcggatc agtcatgatg ctgccgcttg cagtttccac aaacgaatag
     1141 gtattattgc tcctgcgtgc agagaatcca atttgtgcaa atgaaaaatc gtagcgcaat
     1201 cccaattcca ggttagttat gctgttcagg aaattccttt caaaggatac gttcaggtat
     1261 ccgttgttga acattctttt ttccagggct gcacgtacag aaacaaactt gccctgggtg
     1321 tagttgaatt gagtctgggg agtcagtatg aatgctgccg gaagttttat ggacatagac
     1381 agggtactgt atatattggg cttggaggca gaggagaatg tcgcgaaagt agtcaggttt
     1441 gtattgatac caaaaaggct tcctgacaac agtaattctg ccgtggtata ctgtgtggta
     1501 ggcatgatca cctggttaag tgacattctt gtgaagacgg aaaaactttt gaacctgatg
     1561 gggattgaca tggcaatgcg cctttcttcc aggtaattgg tgttgatggc cttttggtcc
     1621 ttatcgtaac gtgtgtaatt cacttccagc tgaatgtcag agcgaaacct ccagttcaaa
     1681 atccctttga cattcacacc atgggtatat tcaccggtaa gcagcaatcc cggggcaagt
     1741 ctcagggatg tatgcacgaa gggcatgatc tggccggagg ttacagaaga aaggtattca
     1801 acaccggctc ctatggtcat gaaacgtgtt atcccgtaat ccatgtccag cctggaaaat
     1861 ttactccaca gggaatccga gaccatacca gccgagagcc ggtactcgaa attgcctgca
     1921 ggaaggaaat tatagggtat attcagcgtt tgttccttag agcgttcttc cccccacgga
     1981 ccgtagaatt gtagtttcac attggtgttt ccataaatca tgggaacctc aaacgtatag
     2041 aagcccgatg cgtcagcttg cttgtaatct accaggacat tgttaacata cagttccaca
     2101 gtccagccag gttcagtaac atcgctcagg gtataggtcc cgaaggactg ccggtaagtg
     2161 gtgggtgtgt tggtgatctg tatacccaat accggcgaat ttatggtaga cgtggagaag
     2221 gagttgattt ttcccaggat gacctgacgg agtgctttat atttgttatt ggcataacgc
     2281 cactggtaga actggttcct ttcctgaaag ggagtattgg ttgtgtagtt caggttgata
     2341 ttcgtttctc cgcccaaaat gacgccgcca aggttcaggg acaggcgtgc tcccttaggg
     2401 cccttgattt cttgtgtcag gatcgcagac cagtcggcca tcccaaagtg aatcaatggg
     2461 aatttcctgc caataaccgt atcggctact atgtcacctg ttatctgtcc caggcttttt
     2521 cgcatttgtg ccagtcgcat ttccctgatt accgggagct cgatactggt gttcatggaa
     2581 acggagaggc tcctgtagct gaagctgcag tccaggccga acacctgccc gaagagttct
     2641 gtttttaaga ataaattggt ttctgtccgg atgatatctc cggctttaag ctggaattct
     2701 ttatccttga atgttatcct gttgtttacc cggtctatgt tgtaggcggc atcttcggta
     2761 ataaagaacc cgtttacaga ttcaaatccg gcggtatatg aatttttgat cctaagaaaa
     2821 tcgaatactt caatcaggga taaataaaca ctggtttctc caataacggc tgaaatatct
     2881 gtacccccaa ttcggggaac agaaaaataa atgaccattt cttccagata ctcttcttcc
     2941 tgggccaggc ttctggccgg cagcatgatc ccggtgatcg ccattaaaca agtgatgatc
     3001 gggataagac gcctgaa
//
LOCUS       DAOSOF010000186         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_33578_length_2950_cov_5.157513, whole genome shotgun sequence.
ACCESSION   DAOSOF010000186 DAOSOF010000000
VERSION     DAOSOF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33578_length_2950_cov_5.157513"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLM68_08395"
     CDS             complement(<1..331)
                     /locus_tag="PLM68_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HOT55411.1"
                     /translation="MKKSFFLLVLLTACTSGGQVVVNQLGYYPGQEKIAIVDASFQGS
                     FRVVDHQKGTTVFEGTAGEVFSSLFSDKERTRLDFTSLDSSGIYRIVTEQGMESPAFR
                     IGDSILAP"
     gene            complement(591..2189)
                     /locus_tag="PLM68_08400"
     CDS             complement(591..2189)
                     /locus_tag="PLM68_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOT55412.1"
                     /translation="MLLPVSCSRNNGSSDPDAQQLFMGENIAVAPTVYGKVKGYILRD
                     IYHFMGIPYGASTEGKNRFMPPCPPEPWDEVLPCVYFPTSAPQVVRYSRNSESYSAFV
                     DRWNYDEIGEDCLRLNVWTPALDGNKRPVIVWLHGGGFSSGNSIEQNGYGGENLARYG
                     DIVFCSVNHRLNAFGFSDFSAVGGEAFKNSGNVGLLDIIAALEWIRDNIANFGGDPGN
                     VTIIGQSGGGSKVSAIATMPAAKGLVHKAVALSGNSIKAGNPEFSAGLGACILEEAGL
                     QPSQINQLQEMPWEEYLALANRAATKFNSRMEGLGIRGGFGPVADGTVLPAEIYFSAD
                     DPSIPDIPMIYCTTFHEWNPNRDRPELEDVSLEEVTENLKEHYADKALEIVAAYAGQF
                     PDKKPIEIWALISSSRVNVVRAANTKLQQQSPVYVAWFGWQPPLFDGRLRAFHCIDIC
                     FWFCNTDLMVTHTGGGKEPRMLSRKMADALLRFMRTGNPNGGSLPFWPEYTIGKGETM
                     VLNTHPEVVNDPDREARLVLERAM"
     gene            complement(2234..>2950)
                     /locus_tag="PLM68_08405"
     CDS             complement(2234..>2950)
                     /locus_tag="PLM68_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOT55413.1"
                     /translation="DITRITKNETIMWARSAWAGSQRYPLHWGGDAANTDIGMLTTLR
                     SGLSLGLCGFSFWSHDIGGFVQSCPEELYSRWLPFGFLTSHTRIQGAPPTEPWYYSKD
                     FMDGFRRSAELKYRLMPYIVEQSEECTRTGLPMLRALLIEYPDDPVAWQIEDQYLFGS
                     DIMVAPLFESDKERLVYLPVDRWVDYQSGKTYDPGWHRITTGQIPALILVRKGAVIPQ
                     VPVAQSTDKIEWEKVKNIKY"
BASE COUNT          739 a          761 c          712 g          738 t
ORIGIN      
        1 ggggggccag gatgctgtcc ccgattctga aagcaggcga ctccatccct tgttcggtta
       61 caatacggta aattccggag ctgtctaaag aagtgaaatc aagccgggta cgttctttgt
      121 ccgaaaagag cgaggagaac acttcaccgg ctgttccctc aaataccgtt gtccctttct
      181 gatgatcaac gacccggaaa gagccctgga atgatgcatc cacaatggct attttttcct
      241 gtccgggata atagccaagc tggtttacaa ccacctgtcc gccagatgtg caggcggtca
      301 gaagaacaag aagaaaaaat gattttttca ttatctttac tttggttatg acaaatttat
      361 caaacttaat atgaaaacac taagatcttt agtaaccgaa gtgcttattg catccctttt
      421 cctgagcggt tgcgggacta taggcagaac aggcgtagga gccggctgct aacaataata
      481 caatagaagg caggatgcaa aaccgccgtg tggagattta catcctgcct aatgaaaaga
      541 tgattgaaga agtacagcgc caggctgaaa cttccaaata actccaggga ctacattgcc
      601 cgctccagga ccaggcgtgc ttccctgtcc ggatcgttga caacttcagg gtgggtattc
      661 aacaccatgg tttccccttt cccaatggta tattcgggcc agaagggcag tgaacctccg
      721 ttgggatttc ccgttctcat gaaccgtagc agggcatcgg ccattttcct tgaaagcatt
      781 ctgggttcct taccacctcc ggtatgggtg accataaggt ctgtgttgca gaaccagaaa
      841 cagatgtcaa tacaatggaa tgcacgcagc cgtccgtcaa acaaaggcgg ttgccatccg
      901 aaccaggcta catagacggg ggattgctgc tgcaacttgg tgttggctgc acgcaccaca
      961 ttcacccggc tggaactgat aagggcccag atttcgattg gttttttatc tgggaactgg
     1021 ccggcataag cagccacaat ttcaagggct ttgtctgcat aatgttcctt aagattttcc
     1081 gttacctctt ctaatgatac atcttccagt tccggacggt ccctgttggg attccattca
     1141 tggaacgtgg tgcagtatat catgggaata tcgggaattg acggatcatc tgccgaaaaa
     1201 tagatctcgg caggcagtac tgttccgtct gccaccgggc cgaatcctcc cctgatcccc
     1261 agtccttcca tccggctgtt gaatttcgtg gctgcacggt tggcaagggc aaggtattcc
     1321 tcccagggca tttcctgtag ctgattaatc tgtgaaggct gcaaaccggc ttcctccagg
     1381 atgcaggcgc ccaagcctgc cgaaaattcc ggatttcctg ccttaatgga gttaccgctt
     1441 agtgcaactg ccttgtgaac cagtcccttg gcggcaggca tggtggcaat ggcacttact
     1501 ttggaaccac cgcctgattg cccaatgatg gttacattgc ccggatctcc accaaagttg
     1561 gcaatattgt cccggatcca ttccagggca gctatgatat ccagcagtcc cacattaccc
     1621 gaattcttga atgcttcccc gcctacagca gagaaatctg aaaagccaaa tgcgttcaga
     1681 cggtgattga cagaacaaaa tacaatatcc ccgtaacggg ccaggttctc gcctccatat
     1741 ccgttctgtt caatggaatt accacttgaa aaaccgccac cgtgcaacca tacaatgact
     1801 ggtctcttgt tcccgtcaag ggccggggtc catacattga gccgcaggca gtcttctcct
     1861 atctcgtcgt aattccacct gtccacaaag gcagaatagg attcagaatt gcgggaatac
     1921 ctcactacct ggggagccga ggtcggaaaa tagacacagg gaagcacttc gtcccagggt
     1981 tcggggggac agggtggcat gaacctgttt ttcccttcgg tgctggctcc gtaaggaatt
     2041 cccataaaat ggtaaatgtc ccgtaaaata taacccttca cttttccata aacagtgggg
     2101 gccacggcaa tattctcacc cataaagagt tgctgcgcat cgggatctga agaaccgttg
     2161 ttccgggagc aggatacagg gagcagcatg atcagcgcca gaaggctgta ggcataacgt
     2221 ttcatataca acactagtat ttaatatttt taactttttc ccattcgatt ttatctgtac
     2281 tttgagccac agggacctgt ggtataacgg cccctttcct gaccaggatc agggcaggga
     2341 tctgccctgt ggttatcctg tgccagccag gatcataggt cttgccgctc tgataatcta
     2401 cccagcgatc aaccggaagg taaaccaggc gttccttatc gctttcaaac aagggggcca
     2461 ccattatatc gcttccaaaa agatactggt cttctatctg ccaggccacc gggtcatcgg
     2521 ggtattctat aagcagtgcc cgcaacatgg gcaggcctgt ccgtgtacat tcttcggatt
     2581 gttccacgat atagggcatc agacggtatt ttagttctgc cgatctgcgg aaaccatcca
     2641 taaagtcttt gctgtaatac catggctcgg tgggtggtgc accctgaata cgtgtatggg
     2701 acgtcagaaa accaaacggg agccagcggc tgtataattc ttccggacaa ctttgtacaa
     2761 aaccgcctat gtcatgactc cagaaagaaa atccgcacaa tcccagggaa agtccgcttc
     2821 tcagggtggt aagcatacca atatcggtat tggcagcatc ccctccccaa tgcagcggat
     2881 agcgctgtga tcctgcccag gcacttctgg cccacatgat cgtttcattt ttggttatgc
     2941 gggtgatgtc
//
LOCUS       DAOSOF010000187         2922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_33983_length_2922_cov_5.403907, whole genome shotgun sequence.
ACCESSION   DAOSOF010000187 DAOSOF010000000
VERSION     DAOSOF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33983_length_2922_cov_5.403907"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /locus_tag="PLM68_08410"
     CDS             complement(<1..2373)
                     /locus_tag="PLM68_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT55414.1"
                     /translation="MKENYLKELNEPQRQAVEDIKGPSLIIAGAGSGKTKVLTCRIAH
                     MLEQGIPASAILALTFTNKAAKEMKDRVSSLVGSEKVRYLWMGTFHSVFARILRTEAQ
                     VLDFPRNFTIYDKSDSKSAIKSCIKELKLDEKVYPPSEVMSRISRAKNNLMTASAYAA
                     DDQAMQADEAARKPRMADIYALYARKCRLAGAMDFDDLLLYTNILFQQNPQILALYRD
                     RFRYILVDEYQDTNLAQYLIITALAREHRNIAVVGDDAQSIYAFRGARIENILNFSKD
                     FPEAKEYRLEQNYRSTQTVVNAANSLIKHNARRMRKECFAQSETGEKIALLCAYTDAE
                     EAFLVVSSILDKVYRSREGYGEFAVLYRTNAQSRLLEEALRKRNIPYKVYGGFSFYER
                     AEVKDMMAYMRLVVNPDDEEAFRRAVVTPSRGIGDVSLQKLGTAALFAGMSSIGYIRQ
                     GDLEAAGLRGNIAGRLKAFSEMIETVYNMQEEGANAYQVAMEVARLSGYLPMLREDKS
                     IEGMSRLNNVEELLNSIQAYCGMAAEGVELETEVSVPDAGEAAPTLERFLKNVTLLTQ
                     MDGQEQPDNERVSLMTVHSAKGLEFSHIYVVGMEEKLFPSLLSSNTQTELEEERRLFY
                     VAMTRAKKSLTLSFSRTRMHWGNMESNPPSRFLREIDPGFLNQPVPDVFGSGTEEQEG
                     RSTWSREGKFGSGGGKFGSREGKFGSGGGRAGFEGGNTWAGRRNSFGASTPADRPGGQ
                     GFKAGGASARDSFKAPGGTSGKEGFSTPGGTPGPADFQAADASSLTVGMYI"
     gene            2437..>2922
                     /locus_tag="PLM68_08415"
     CDS             2437..>2922
                     /locus_tag="PLM68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOT55415.1"
                     /translation="MSACKKLLRGLDIPLKGSAHPMTVSLELSSLYTLKPSDFKNTTP
                     KLLVREGDNVQAGSPVFCDKEHPEVLFTAPVSGTIHRIVRGEKRKITGIQILAGDEQM
                     QEQFPVAEPEQLSPGTIRELLLQSGCWPLITRRPYGITALPGETPKAIYISCFDSAPL
                     AP"
BASE COUNT          635 a          808 c          731 g          748 t
ORIGIN      
        1 aatatacatt cccacggtaa gggacgaggc atccgccgcc tgaaaatctg ccggacccgg
       61 ggtaccaccg ggtgtgctga atccttcctt accagaagtg ccaccgggcg ccttgaagct
      121 atccctggcc gaagctcctc cggccttgaa tccttgtccg ccggggcggt ctgcaggagt
      181 actcgcgcca aatgagttgc gccggccggc ccaggtgttt ccgccttcaa aaccggcccg
      241 tccgccaccg gacccgaact ttccctcacg ggatccgaac tttccaccac cggacccgaa
      301 ctttccctca cgggaccaag tgcttctacc ctcctgttct tcggttccgg agccaaatac
      361 atcgggaacc ggctggttaa ggaaaccggg atctatctca cgcagaaagc ggctgggcgg
      421 gttggactcc atgttgcccc agtgcatacg ggtgcgtgaa aaggaaagag tgagtgattt
      481 cttggcccgg gtcatggcca cataaaacaa ccgcctttct tcttccagtt cggtctgggt
      541 gttgctggac aacagggaag gaaataattt ctcttccatc cccaccacat agatgtggga
      601 aaactccagg cctttggccg aatggactgt catcaggctc acccgttcgt tgtcgggctg
      661 ttcctgtccg tccatctgag tgagcagggt cacgttcttc aggaatctct ccagggtggg
      721 agccgcttct cccgcatcgg gcacactcac ttctgtttca agttccacac cttctgctgc
      781 cataccacaa taggcctgga tgctgttgag cagttcttcc acgttattca atctggacat
      841 gccttctatg gatttgtctt ccctgagcat gggaaggtaa cccgataacc gtgccacttc
      901 catggccact tggtaggcat tggctccttc ttcctgcata ttgtacacgg tctcaatcat
      961 ttctgaaaaa gctttcaggc gtccggctat gttgcccctc agccctgccg cttccaggtc
     1021 gccctgccgg atatacccta tggaggacat gccggcgaac agtgcggcag ttccaagttt
     1081 ctgtagggac acatcgccaa tgcctcttga cggcgtgact actgccctgc gaaaggcttc
     1141 ttcgtcatcg gggttgacta ccaggcgcat gtatgccatc atgtctttca cctcggccct
     1201 ttcgtaaaag gaaaatccac cgtatacctt ataagggatg ttccttttgc gcagcgcctc
     1261 ctccaggagt ctggactgcg cattggtgcg gtacaggacg gcgaactccc cgtaaccctc
     1321 tcgggatctg tatactttgt ccaggatgga tgagaccacc aggaatgctt cttctgcatc
     1381 ggtataggca cacagcaagg caatcttttc cccggtctcg gattgtgcaa aacattcttt
     1441 gcgcatgcgg cgcgcattgt gttttatgag gctgttggca gcattcacta cggtctgggt
     1501 ggagcggtaa ttctgttcca gcctgtattc tttggcctcg ggaaagtcct tggaaaaatt
     1561 caggatgttt tcaatcctgg ccccgcggaa tgcataaatg ctctgggcgt catcacccac
     1621 tacggctatg tttcggtgtt cccgggcaag ggctgttatg atcaggtact gggccaggtt
     1681 ggtgtcctgg tattcatcta ccaggatata gcggaaacgg tccctgtaca gggccagtat
     1741 ctgcggattc tgctgaaaaa ggatattggt gtagagcaac aggtcgtcaa aatccatggc
     1801 cccggcaaga cggcactttc ttgcatacaa ggcgtaaata tcggccattc ggggtttacg
     1861 ggccgcttca tcggcctgca tggcctggtc gtcggccgca taggcagaag ctgtcatgag
     1921 gttgtttttg gccctggata tgcgtgacat cacctctgag ggaggataga ccttttcatc
     1981 cagtttcagt tctttgatgc agcttttaat ggcagatttg ctatcggatt tgtcataaat
     2041 ggtaaaattc cgcggaaaat ccagtacctg tgcttctgtc cgtaaaatgc gcgcaaaaac
     2101 cgagtggaaa gtacccatcc acaggtaccg gactttttca gatccgacca gtgaagacac
     2161 tcggtctttc atctctttgg ctgccttgtt ggtgaatgtc aaagcaagta ttgcagaagc
     2221 ggggatcccc tgttccagca tgtgagctat cctgcatgta agtactttgg ttttaccact
     2281 accggcaccc gcgatgatca acgatggacc ttttatatct tcaactgctt gtctctgagg
     2341 ttcattcaac tccttcaaat agttctcctt catgtcgaca aatatagata aaaatgccga
     2401 tgaaaatttt atctttgcat ttgctaatct acgtatatgt ccgcctgtaa aaagttgctc
     2461 agggggttgg atattcccct caaaggcagt gcccacccta tgaccgtctc tttggaactt
     2521 tcctccctgt acacactgaa accttccgat ttcaaaaaca ccaccccgaa acttcttgta
     2581 agggaaggag ataatgttca ggccggctcc cctgtattct gcgacaagga acatccggaa
     2641 gttttattca cggcccctgt cagcggcacc atccacagga tagtccgtgg tgaaaaaaga
     2701 aagatcaccg gcattcagat tcttgccggt gatgagcaga tgcaggagca gtttcccgtt
     2761 gccgaaccgg aacagctttc cccggggacg atcagggagc ttttgctgca aagcgggtgc
     2821 tggccgctga tcacccgtag accgtatggc attactgccc tccccggaga aacaccaaaa
     2881 gccatatata taagctgttt tgactcggcc ccgcttgcac cc
//
LOCUS       DAOSOF010000188         2887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_34539_length_2887_cov_4.260946, whole genome shotgun sequence.
ACCESSION   DAOSOF010000188 DAOSOF010000000
VERSION     DAOSOF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34539_length_2887_cov_4.260946"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLM68_08420"
     CDS             complement(<1..614)
                     /locus_tag="PLM68_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55416.1"
                     /translation="MDPIATTVPVTGLKAPVEFQVLRVPDHFTKADFSTHRQADFKGI
                     TSPDCNMVTSSQIQYYHPDNLPARFLCFFPEPDTLINGVASFTLDGTQDIIYSPVAYA
                     GSLYAPNPDFGYSFNHELSRLNVTVTLSQDMDMTEDFVLLSAEVLTYNKLQLQLGGPL
                     AGQLKVAGDAKKVLIPLRDDIGSITRNIRLSKNPEDCGSVYAYAG"
     gene            complement(836..1651)
                     /locus_tag="PLM68_08425"
     CDS             complement(836..1651)
                     /locus_tag="PLM68_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase I, inosine and
                     guanosine-specific"
                     /protein_id="HOT55417.1"
                     /translation="MKTSVYQESADYILKKIPKRPQVAIILGSGLGNLAESIQDQVVI
                     PYAEIPNFLLSTAPGHKGNLVWGRLGGKEVICMQGRFHYYEGYGMEQVTLPIRVFKLL
                     GAEALLVSNAAGGINPRFKQGDLMVITDHINLMPNPLIGKNNPDFGDRFPDMTRTYDK
                     ELRKICDDIAHIHGIPLRHGTYIGSSGPTFETPAEYNFFKSIGADATGMSTVPEVIVA
                     RHCGMRVFGMSVITNEAYDFAEDFENNGEDVIAVADKAAAKMALLFADIIANM"
     gene            complement(1648..>2887)
                     /locus_tag="PLM68_08430"
     CDS             complement(1648..>2887)
                     /locus_tag="PLM68_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT55418.1"
                     /translation="DVLIEGNKIKSVESAGSIPEQGIEKVIDGSRKAIIPGFVNAHAH
                     SAMTLFRGVGEDVPLDQWLRERIWPYEAKLDEEMVYWGARLACIEMIRTGTTTFNDMY
                     WHLNGTKRAAEDMGMRSVQSFTWLDHFDDRVIEENKKLLKEAHKASSGWSSLTAFSVS
                     PHAIYTVAEKSFRYINSYARDNNLTVHTHLAETRSEMDQAEREFGMSPTEYLNKLGLM
                     SPNLVAAHSLWLSSDDIKILGDNGVNTVHNPNSNLKLASGYKFKYNELRDAGANVCLG
                     TDGCASSNNLDMIDSMKTAALLQKVWRNDPGAIPLKELMNVASLNGAKALHLNAGKIE
                     PGYLADIALVDMAKPSFVPNHYFLANLVYAANGECIDTVICDGRIIMENQQIPGQEEI
                     YRKVQEVADKVVDYKKRKKK"
BASE COUNT          676 a          715 c          725 g          771 t
ORIGIN      
        1 ccggcataag catatacaga cccgcaatcc tcggggtttt tagagagacg tatgttcctg
       61 gtaatgctgc cgatatcatc ccttaaggga atcagaacct tttttgcatc cccggcaacc
      121 ttcagctggc cggcaagcgg tcctcccaac tgcaactgca atttgttata agtgagcact
      181 tcagcagaaa gcagcacaaa atcttcagtc atgtccatat cctgagagag tgtgactgtg
      241 acattcagcc gggacaattc gtgattgaaa ctgtatccga agtccggatt gggagcatac
      301 aggctcccgg cataagcgac cggtgagtaa atgatgtcct gtgtgccgtc cagggtgaaa
      361 ctggcgactc cgtttatgag ggtatcgggt tccgggaaaa aacacaaaaa acgggcgggc
      421 agattatccg ggtgataata ctggatttgt gaagaggtca ccatgttgca atcaggagat
      481 gtgattccct tgaagtctgc ttgacggtgt gtggaaaaat ccgctttcgt gaaatgatcc
      541 ggtaccctga gcacctggaa ttccacagga gcttttaggc cggtcactgg tacggtagtc
      601 gctatgggat ccatagaatt atctcggata tctgatgtgc attgtataaa taacaactgc
      661 acgacgatca ggataaataa tgatcgcatc gtatgtagga ttaaacgtac gatgtaaatg
      721 taaggaaaaa aaatgaatta tgctacatgt tttttaagaa aaatgcatat aaatgtgcta
      781 tttctccgac atccctaccc cacgcaagag tacgcagtac tccagggcag gatccctaca
      841 tgtttgcaat gatatcagca aacaagagtg ccatttttgc ggcagcctta tcggcaactg
      901 caatgacatc ttccccgtta ttctcaaaat cttcggcaaa gtcatacgct tcattcgtta
      961 tgacagacat gccaaaaacg cgcatgccgc aatggcgtgc cacaatgact tccgggacgg
     1021 tggacatccc ggtggcatct gccccaatgg acttgaaaaa attatattct gccggggttt
     1081 caaaagtagg tccgctgctg ccgatatacg tcccgtgacg cagcgggatg ccgtggatat
     1141 gggctatatc gtcacatatt ttgcgtaact ccttgtcgta ggtgcgcgtc atatcgggaa
     1201 aacgatctcc gaaatcaggg ttgttcttgc caatgagcgg attgggcatt aaattgatgt
     1261 gatctgtaat gaccatcagg tctccctgct tgaagcgcgg attgatcccg cctgccgcgt
     1321 tggaaactaa gagcgcttct gctcccagca gtttgaagac gcgtatgggt aaagttacct
     1381 gctccatgcc gtatccttcg tagtagtgaa agcgcccctg catgcagatc acttcttttc
     1441 ctcccaggcg gccccagacc aggtttcctt tatgcccggg agcggtggat agtaaaaaat
     1501 tggggatctc ggcataaggt atgacaacct ggtcctgtat gctttctgcc aggtttccaa
     1561 ggccgctgcc aaggataatg gccacctgcg gacgttttgg aatctttttg agtatgtaat
     1621 ctgctgattc ctggtaaact gaggtcttca tttctttttt ctttttttat aatctactac
     1681 tttgtctgcg acttcctgca ctttacggta tatttcttcc tgtccgggaa tttgctggtt
     1741 ttccatgatg atcctgccgt cacagatgac cgtgtctatg cattccccgt tggccgcata
     1801 gacaaggttg gccagaaagt aatggttggg tacgaaggaa ggcttggcca tgtcaaccag
     1861 cgctatgtct gccaggtaac cgggctcgat ctttcccgca ttcaggtgca aggctttggc
     1921 cccgttcaga gaagccacgt tcataagctc tttcagggga atggccccag gatcgttccg
     1981 ccaaaccttt tgcaaaagag ctgctgtctt catgctgtct atcatatcca ggttgttgga
     2041 cgaagcacag ccatcggtgc ccaggcatac attggccccg gcatctctca gctcgttgta
     2101 tttgaattta taacccgagg cgagcttcag gttggagttg ggattgtgta cggtattcac
     2161 accgttatcg cccaggatct taatatcatc gctgctgagc cagagtgaat gggctgcaac
     2221 caggttgggg ctcatcagac ccagcttgtt caggtattcg gtgggactca tgccgaattc
     2281 gcgctcggcc tggtccatct cggagcgggt ctcggcaagg tgcgtatgca ctgtaaggtt
     2341 gttgtccctg gcatagctgt ttatgtaacg gaaagatttc tctgccacgg tgtatatggc
     2401 gtggggactg accgagaatg cggtgagtga actccatcca ctgcttgcct tgtgggcctc
     2461 tttcaacaat ttcttgtttt cctcgatcac acggtcatcg aaatgatcca gccatgtaaa
     2521 agactggaca gaacgcattc ccatatcttc tgccgcccgc ttggtcccgt taaggtgcca
     2581 gtacatatcg ttgaaggtgg tggtgcctgt gcggatcatt tctatacagg ccagtcttgc
     2641 accccaatag accatttcct cgtccagctt ggcttcatac ggccagatgc gttcgcgcag
     2701 ccactggtcc aggggaacat cctctcccac cccgcggaat agcgtcatgg ccgaatgggc
     2761 atgggcattc acaaaaccgg gaataatggc cttgcgggat ccgtcaatca ccttctcaat
     2821 gccttgttcc ggaattgagc cggcggattc aacactcttt atcttgttgc cctctatcag
     2881 aacgtcc
//
LOCUS       DAOSOF010000189         2874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_34753_length_2874_cov_3.443065, whole genome shotgun sequence.
ACCESSION   DAOSOF010000189 DAOSOF010000000
VERSION     DAOSOF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34753_length_2874_cov_3.443065"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLM68_08435"
     CDS             complement(<1..984)
                     /locus_tag="PLM68_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55419.1"
                     /translation="MKYRGLILALLILSHLAGCVKVSSLDDRASVASCLITDVFPEAV
                     VFNEPVIEEDAIVLPMDYGKYEFPVTVTLDIQTAQAIDKILGMDTGNTLVFEDHETMR
                     RIHLIAQSGVVHTYSIRIEVAPRSDQAAVTAARLTAYSPAEFPLAGEVVVDIVENEIG
                     IYALKGAPLPLTIGLELDLSPGAALEKPGKNGNFTIDAYDTSIPFTVVAESGKKELWH
                     IRLIGVVLAEDASQTDREPWERMAPGGALEIAFKPGEVECVGITADLQTSRYKVSVRD
                     NGIPFPWKALVGFKMNPYVVPIHHDTLEEFTIQNWEDKRTFYLVDILSRQAR"
     gene            complement(984..>2874)
                     /locus_tag="PLM68_08440"
     CDS             complement(984..>2874)
                     /locus_tag="PLM68_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT55420.1"
                     /translation="NKEIPIIADDYVTMDFGTGCLKITPAHDPNDYEIGLRHKLPVID
                     ILNLDGTLNGEARILVGEDRFVARKKILIMLEEAGCLEKEESYVTQIGFSERTDAVIE
                     PRLSQQWFVKLSILAKKALEYVEDGQVRLIPDKYRNTYRHWMETARDWCISRQLWWGQ
                     QIPAYYLPGGEVVVASSEEEALVLARKIDSTLKAGDLKQDPDVVDTWFSSWLWPISVF
                     DPEKPGHPEKEPNQELAYYYPTDDLVTAPDILFFWVARMIMAGDLFCGQKPFSNVYLT
                     GMVRDKMGRKMSKSLGNSPDPIELMNIYGADGVRMGLMLCTSAGNDILFDETQMEQGR
                     NFCNKIWNAFRLVSSWQRDEHLEQEETSRQAVRWFGQLLSATRDEAEDLFSKYRLSEV
                     LMLLYKRFWDDFCSWYLQIIKPAGNAGKRIDAATYDSTLAFFDLLLHLLHPFMPFITE
                     ELWQNMAKRKEGDSIMVSPLPASGPCNQAELEDFSRLQELIVSIRGLRQNRQIPGKTL
                     LKLYVKGQFPQILHGIARRMGGLESVQMVTEAPQEGNVYAFLVGTTVFFIPLWNLLDK
                     EEERSKLNAELEYHRKFLAIVDKKLSNESFLQRAPADVVAIERKKRSDAMSRIAALKQ
                     QLDLL"
BASE COUNT          690 a          744 c          677 g          763 t
ORIGIN      
        1 ccgtgcctgt cgtgaaagta tatcaaccag gtaaaaggtt cttttatctt cccagttctg
       61 gattgtgaat tcttccaggg tatcatggtg aattggcacg acatagggat tcatcttaaa
      121 acccaccagg gctttccagg gaaaaggaat gccgttgtcc cggacagaaa ccttgtaacg
      181 gctggtttgc agatcggccg ttattcccac acattccact tcacccggtt taaatgcaat
      241 ttccaaagct cctccagggg ccatgcgctc ccagggttcc ctgtctgtct gcgatgcatc
      301 ttctgccaat acaaccccaa tcaggcgaat gtgccacaat tctttcttcc cgctctcggc
      361 aactactgtg aaagggatag acgtatcata ggcatctatg gtgaagttac cattcttgcc
      421 aggtttttcc agtgcggcac cgggactcag gtccagctcc agacctattg taaggggcaa
      481 gggagctccc ttcagggcat agatacctat ttcattctcg acaatatcca ccacaacttc
      541 ccctgccagt ggaaattctg cgggactata cgctgtaagc cgggcagctg taactgcagc
      601 ctgatcgctc cggggtgcca cttcaatacg tatgctgtat gtgtgtacta ccccgctctg
      661 ggcaatcaga tggatcctgc gcatggtttc gtgatcctca aataccagtg tattgccggt
      721 gtccatgccc aggatcttgt cgattgcctg ggctgtttgt atatcgaggg tgactgtaac
      781 cggaaattcg tattttccgt aatccattgg caacacaata gcatcctctt caataaccgg
      841 ttcattgaag acaacggctt ccggaaacac atctgtaata aggcaggatg ccacagatgc
      901 acgatcatca agactggaca ctttaacaca gccggccaga tgtgaaagaa tgagcaatgc
      961 aaggattaaa cccctgtatt tcattataat aaatctaatt gttgctttaa ggctgcaatc
     1021 ctgctcatag catccgaacg ttttttacgt tcaatggcaa caacatccgc gggagcacgt
     1081 tgaaggaaac tttcattgga taatttcttg tccacaatag caagaaattt ccggtgatat
     1141 tccagttctg cgttaagttt agaacgttct tcctctttat ccagcaggtt ccacagcgga
     1201 atgaaaaaaa cggtcgttcc caccaggaag gcataaacat tgccttcctg cggtgcttcg
     1261 gttaccattt gtacagattc cagtccaccc atgcgccggg caattccatg caggatttgt
     1321 ggaaactgtc ccttgacata gagtttcaaa agtgtttttc cgggaatctg cctgttttgc
     1381 ctgaggcccc tgatggacac aatcagttcc tgcaaccggg aaaaatcctc aagttctgct
     1441 tggttgcagg gtcctgaagc cggtaagggg gacaccatga tactgtcccc ttccttacgt
     1501 tttgccatat tttgccaaag ttcctcggtt ataaacggca tgaacggatg cagcaggtga
     1561 agcagcaggt caaaaaatgc cagcgtgctg tcgtaagtgg ctgcatcaat tctcttgcct
     1621 gcattcccgg caggtttgat gatctgcagg taccaggaac agaaatcgtc ccagaaacgc
     1681 ttgtatagca gcatcaggac ttctgagagc ctgtatttgg aaaacaggtc ctcggcttca
     1741 tccctggttg cagagagtaa ttggccgaac cagcgtacag cctggcgcga tgtttcttcc
     1801 tgctccaggt gttcatcgcg ttgccaggaa ctcaccagac ggaaggcatt ccagatcttg
     1861 ttgcagaagt tccggccctg ttccatctga gtttcatcaa acaagatatc gtttccggca
     1921 gaagtacaga gcataaggcc catacgcaca ccgtctgcac cgtaaatatt catcaattca
     1981 atgggatccg gtgagttgcc cagagatttg gacatcttgc ggcccatttt gtcgcggacc
     2041 atccctgtga ggtaaacatt ggaaaagggt ttctggccgc agaacagatc cccggccatg
     2101 atcatcctgg ctacccagaa gaaaaggata tccggggcag tgaccaggtc atctgtggga
     2161 tagtaatacg ccaactcttg gttgggttct ttttccgggt ggcccggttt ttcgggatca
     2221 aagaccgaga tgggccagag ccacgaggag aaccaggtgt ccaccacatc gggatcctgc
     2281 ttcagatccc cggcttttaa cgtggaatca attttccggg ccaggacaag cgcttcttct
     2341 tcagacgaag cgaccaccac ttctccgccg ggcaggtagt aggccgggat ttgctgcccc
     2401 caccacaatt gtcttgaaat gcaccagtcc ctggctgtct ccatccagtg acggtatgtg
     2461 ttgcggtatt tgtcagggat aagccttacc tgtccatctt ccacatattc taaagccttt
     2521 ttggccagta tgctcaattt aacaaaccat tgctgcgaga gccgtggttc aatgaccgca
     2581 tctgtacgtt cagaaaatcc aatctgagtg acataggatt cttctttttc caggcatcct
     2641 gcctcttcca gcataatgag gatttttttg cgtgccacaa agcggtcttc ccctaccagg
     2701 atcctggcct ccccgttcag ggtgccatcc aggttcagaa tatctatcac aggcagtttg
     2761 tgccgcaatc ctatttcata gtcattggga tcatgggccg gggtgatctt taggcaccct
     2821 gttccaaaat ccatggtaac gtagtcatcg gcaataatgg gtatttcctt gttt
//
LOCUS       DAOSOF010000190         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_35420_length_2832_cov_4.928700, whole genome shotgun sequence.
ACCESSION   DAOSOF010000190 DAOSOF010000000
VERSION     DAOSOF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35420_length_2832_cov_4.928700"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLM68_08445"
     CDS             <1..440
                     /locus_tag="PLM68_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT55421.1"
                     /translation="FLDSSDPGKDIQIYINSPGGSVTAGLGIYDTVQLVKSDVATICT
                     GQAASMAAVLLAAGEKGKRSALPHARVMIHQPVGGVEGQAADMAIAVKEILELQKQLY
                     QILAQHTGKTLEQIQKDADRDFWMRSTDAVDYGIIDEVLRKAK"
     gene            443..1678
                     /gene="clpX"
                     /locus_tag="PLM68_08450"
     CDS             443..1678
                     /gene="clpX"
                     /locus_tag="PLM68_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT55422.1"
                     /translation="MQKKTPDTDYCSFCGKSSKETGVLITGIDASICELCLQEASLVL
                     DDMYGHKSKNPSKKDPVPNGRLMTPREIRAFLDEYVIGQDQAKIFLSVAVYNHYKRIR
                     SGKGNDVDIEKSNILLVGPTGTGKTLLANTIARMLNVPFTIVDATVLTEAGYVGEDVE
                     SILTRLLQASDYDVDRAEQGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEG
                     TVVNVPPQGGRKHPEQRMIAVNTKNILFICGGAFEGIERKIAQRLNTSVVGYGMAEKK
                     AHIDKDNLVKYVAPQDLRAYGLIPEIVGRVPVVTYLNRLDREALKDILTKPKNALVKQ
                     YKHLFALDGIKMSIDPQVLDYIVDTAIEFKMGARGLRSICETIMVDAMYDAPSSGKKT
                     LRITLPYAKKRVEKLTKII"
     gene            1689..>2832
                     /locus_tag="PLM68_08455"
     CDS             1689..>2832
                     /locus_tag="PLM68_08455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT55423.1"
                     /translation="MSEIKDFINKNSQRFLDELFTLLRIPSVSSQQEHKEDMQRCARQ
                     YVVSLIEAGADRAEIHSTKGHPVVYAEKIIDSRKPTVLVYGHYDVQPEDPIGLWKTPP
                     FEPEVRDGAIYARGANDDKGQSFMHIKAFEYLVSRDELPCNVKFLIEGEEEIGSPSLG
                     EWARQNKELLACDMILVSDTTMISEEVPSINTGMRGLAYLEVKVTGPDKDLHSGHYGG
                     AIANPINVLCGMIAGLIDNKGRITIKGFYDDVVELSPEDRALLALAPFNLEHYKKSIG
                     INGITGEEGYSTLERIGIRPCLDVNGIWGGYTGEGAKTVIPSFARAKISMRLVPNQDH
                     KKIARLFAEHFTSMAPAGVSVEVTEHHGGQGFLAPISSPMYQAAAKA"
BASE COUNT          762 a          679 c          774 g          617 t
ORIGIN      
        1 ttttcctgga ctcttcggat cccggcaaag acattcagat ctatatcaat tcgccaggtg
       61 gttcggttac agccggattg ggtatctacg acaccgtaca gctagtcaaa tcagacgttg
      121 ccaccatatg taccggacag gctgcatcca tggccgccgt gttgctagca gccggggaga
      181 aaggaaaacg ctcggcattg ccccacgcgc gggtgatgat acaccagccg gtgggcggag
      241 tggaaggaca ggcagccgat atggcaatag ccgtcaaaga gatcctggaa cttcagaaac
      301 aactctatca gatacttgct caacatacgg gcaaaacact ggaacagatc caaaaagatg
      361 ccgacaggga tttctggatg cgttctaccg atgctgtgga ttacgggatt attgatgaag
      421 ttctcaggaa agccaaataa taatgcaaaa gaaaaccccg gacacagatt attgttcttt
      481 ttgcgggaaa tcaagtaagg aaacgggtgt gctcattacg ggcatcgatg catccatctg
      541 tgaactatgc cttcaggaag cgtccctggt gctggatgat atgtatggcc ataagtcgaa
      601 gaatccgtcc aaaaaggatc ctgtgcctaa tggcaggctg atgacaccca gggagatccg
      661 cgcttttctt gatgagtatg tgattggtca ggaccaggcc aagatatttc tttctgttgc
      721 cgtctacaac cattacaaac ggatccgttc cggaaagggc aatgatgttg acatagagaa
      781 atcgaacatc ctgttggtag ggcccacggg aaccgggaaa acgctgctgg ccaataccat
      841 tgcccggatg cttaacgttc ccttcaccat agtggatgcc accgttttga cagaagccgg
      901 ttatgtggga gaagacgtgg aaagcatcct gacccggctg ctgcaggcct cggattacga
      961 tgtggacagg gctgagcagg ggatagtctt cattgacgag atagacaaga ttgcccgtaa
     1021 gagcgacaat ccgtccatta cccgcgacgt gtcgggagag ggagttcagc aggccttgct
     1081 caaattgctg gagggaacgg tggtgaatgt gcctccccag ggaggtcgca aacaccctga
     1141 acagcgtatg atagcagtca atacaaagaa tatcctgttc atatgtggcg gtgcttttga
     1201 gggaattgaa cgcaagattg cccaacgcct caatacgagt gtggtagggt acgggatggc
     1261 agagaaaaaa gcgcacatag acaaggacaa cctggtaaaa tacgtagctc cccaggattt
     1321 gcgcgcatac ggcctgatac cggaaattgt gggccgcgtg cccgtggtca cctacctgaa
     1381 caggctggac agggaagctt tgaaggatat cctgaccaaa ccaaagaatg cattggtaaa
     1441 gcaatacaag caccttttcg cgctggatgg cataaagatg tctatcgatc cgcaggtgct
     1501 cgattacatt gtggatacgg ccattgaatt taaaatgggc gcacggggat tgcgctccat
     1561 ctgtgaaacc atcatggtgg acgccatgta cgatgcgccc tcttcgggaa agaaaaccct
     1621 gcgcattacc ctgccttatg caaaaaaacg tgtggaaaaa ctgacaaaaa tcatataaac
     1681 aggatatcat gagcgaaatc aaggatttta tcaacaaaaa ttctcagcgt tttctggatg
     1741 aactttttac cctgttgcgt attccatctg tcagttcaca gcaggaacac aaggaggaca
     1801 tgcagcgatg tgcccgtcaa tatgtggttt cactgataga agccggggca gacagggcag
     1861 agatacattc caccaaaggt cacccggttg tgtatgcaga aaaaatcatt gattcccgga
     1921 aacctaccgt cctggtttat ggccattacg atgtgcagcc tgaagatccc attggattgt
     1981 ggaaaacccc tccctttgag cccgaggtcc gagacggggc catctatgcc cgcggtgcca
     2041 atgatgacaa aggacagtct ttcatgcaca taaaagcttt tgaatacctg gtttcccgtg
     2101 acgaacttcc ctgcaatgtg aagttcctga ttgaaggtga agaggaaatt ggttctccat
     2161 cgttaggcga gtgggccagg cagaacaagg aattgctggc ctgtgacatg atcctggtct
     2221 cggacacaac catgatcagt gaggaggttc cctccatcaa cacagggatg cgcgggctgg
     2281 cctacctgga agtgaaggtt accggacccg acaaggattt gcactcggga cattatggcg
     2341 gggccattgc caatcccatc aacgtgctgt gtggcatgat agccgggttg atagacaata
     2401 agggccgcat caccataaaa ggcttttatg acgatgtggt tgaactctca ccggaggaca
     2461 gggcactgct tgcccttgcc cctttcaacc tggagcatta caagaaatcc ataggcatca
     2521 acggcatcac aggggaagaa ggctattcca cgctggaaag gatcgggatc cgtccctgcc
     2581 tggatgtgaa cgggatatgg ggcggttata cgggggaagg ggccaagacg gtgatcccgt
     2641 cctttgcccg ggccaagata tccatgcgcc tggtacccaa ccaggaccac aagaaaattg
     2701 cgcgtttgtt tgcagaacat tttacatcca tggcacctgc gggggtatcg gtggaagtga
     2761 ccgaacacca cgggggacaa ggtttcctgg cgcccatatc gtcacccatg taccaggccg
     2821 cggcaaaggc cg
//
LOCUS       DAOSOF010000191         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_37006_length_2740_cov_4.131471, whole genome shotgun sequence.
ACCESSION   DAOSOF010000191 DAOSOF010000000
VERSION     DAOSOF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37006_length_2740_cov_4.131471"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="PLM68_08460"
     CDS             complement(<1..1766)
                     /locus_tag="PLM68_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT55424.1"
                     /translation="MHAMITNREVLARIPYTWKPLDKGYSDQSLYVNLSTFSAEVRPI
                     APEVKEKFIGGKGYGLRLLWDAVTPRTKWSDPENEIVISGGPVCGITQYSGTGKSICV
                     GLSPLTEGVVDSNVGGHFGPHLKFAGFDALEVQGIAPREIILYINGVDHEIEFFVPPA
                     GMHVDSHLLAEELHHMFADSESDKLNISVVSTGQAADHSLIGMLNFSFYDVKRKKIRL
                     KQAGRGGLGTLMRHKNVRAIVARIPKVSGTLNNVVDMPAVLERGRRFTREMNELDDQQ
                     CQMRKVGTAHLMGVMEKYDLLPVMNFKYGDHKDCHKIDMEVWKKHFTQGVPDGCWLGC
                     TMMCAKGVDDFELRTGPYKGSRVLVDGPEYETAAALGSNMAVFDPLYVIEQNFYCDTY
                     GICTISWGNIMAFIMECWEAGVLDKERTGGLDFTWGNAKDALECMHQLARGEGFGLIA
                     GLGVRKMKEMFIEKGWGDASFLNDIAMENKGMEYSMYISKESLAQQGGYAMTNKGPQH
                     DEAWLIFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLAGFCKLCWNDVEPADNAKTAE
                     PAKVPEHVDNYVTVFNAVTGSN"
     gene            1810..2409
                     /gene="thiF"
                     /locus_tag="PLM68_08465"
     CDS             1810..2409
                     /gene="thiF"
                     /locus_tag="PLM68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOT55425.1"
                     /translation="MKVKENIRALLAKKCVGIAGAGGLGSNVASALVRSGLGHLVIAD
                     HDTVSRENLDRQFFFADQEGRPKVYALQDNLQRISSGTRLTLFYGSVTARNARELFSC
                     CHVVVEAFDMAAEKEWFATWMTENLSHIPLVMGSGIAGWGHSDKLRVQRSGNLYVCGH
                     QLDTHETEVPLMAPRVGLVAMMQADIVLEILLTTKAPSQ"
     gene            2406..2609
                     /gene="thiS"
                     /locus_tag="PLM68_08470"
     CDS             2406..2609
                     /gene="thiS"
                     /locus_tag="PLM68_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOT55426.1"
                     /translation="MTITLNNRKETFPGGPMTIARVMEEKSFHFKMLIVKLNGNIISK
                     EAFASTLVENGDRLDIIHLISGG"
BASE COUNT          663 a          736 c          629 g          712 t
ORIGIN      
        1 atgttgctgc cggtcactgc attgaaaacg gtcacatagt tgtccacatg ttcggggacc
       61 ttggcaggtt ctgcggtttt ggcattatca gcaggttcca cgtcattcca gcacagcttg
      121 caaaaaccag ccagtccgaa ccatgtcctg aacatgggaa aatagtgcag agcctctgcc
      181 ttgtcttcaa aggtggggat ctggttattg accatgtcca tgaagatcag ccatgcctcg
      241 tcgtgctggg gacccttatt ggtcatagca tagcctccct gctgggcaag agactctttg
      301 gaaatgtaca tggaatattc catacccttg ttttccatgg caatatcatt aaggaaagag
      361 gcatcgcccc accctttttc aatgaacatt tccttcattt tccttactcc cagtccggct
      421 atcaggccga aaccctctcc ccgggccagc tggtgcatgc attccagcgc atcctttgca
      481 tttccccagg tgaaatccaa acctcccgtc ctttctttgt caaggacccc tgcttcccag
      541 cattccatta tgaaggccat gatatttccc caggatatgg tacagatacc gtatgtatca
      601 caatagaaat tctgttctat gacatagagc ggatcaaaaa cggccatgtt ggaacccagg
      661 gctgcagccg tttcgtactc cggtccgtcc acaagcaccc tgcttccctt atagggtccg
      721 gttctcaact caaaatcatc cacgcccttg gcacacatca tggtacaccc cagccagcat
      781 ccatcgggca ccccctgggt aaagtgtttt ttccagactt ccatatcaat tttatggcag
      841 tccttatgat ccccatattt gaaattcata accgggagca ggtcgtactt ttccatgaca
      901 cccatcaggt gagcagtccc cacctttctc atctgacact gttgatcgtc cagttcattc
      961 atttcgcgtg taaaacgcct gccacgctcc aggacggccg gcatgtccac cacgttgttc
     1021 agtgttcccg ataccttggg tatcctggcc acgatggcgc gcacgttctt gtgccgcatc
     1081 agcgtgccga gcccgcccct gccggcctgt ttcaacctta tctttttacg cttcacatca
     1141 taaaaagaga aattgagcat gccaataagg gaatggtcgg cagcctgccc ggtcgatacc
     1201 acggatatgt tcaatttgtc gctttcgctg tcggcaaaca tatggtgaag ctcctctgca
     1261 agcaggtggg aatcaacgtg catgccagca ggaggaacaa aaaattctat ctcatgatct
     1321 acaccgttga tatagaggat gatctcccgg ggagcaattc cctgtacctc cagggcatca
     1381 aatcccgcaa atttcaaatg cgggccaaaa tgtcccccga cattggaatc caccacccct
     1441 tcggtcaatg gagacagtcc cacgcaaata ctcttgcccg tgcctgaata ctgggtgata
     1501 ccgcatacgg gacctcccga tatcacgatc tcgttctcgg gatcactcca ttttgtccgt
     1561 ggtgtgactg catcccacaa caggcgaaga ccatagccct tgccgcctat gaacttttct
     1621 ttcacctccg gcgctatggg acggacttct gcgctgaacg tactcagatt tacatagagg
     1681 ctttgatccg agtatccttt gtccagcggt ttccatgtat aagggatcct tgccaggact
     1741 tcacggtttg ttatcatcgc atgcataaaa ggtgttttat tgtatatttg tcatgcaaaa
     1801 ataaagaaat tgaaggtaaa agaaaacata agggccctgt tggctaaaaa atgtgtaggc
     1861 attgcaggag caggcggact gggttcgaat gttgcctctg ccctggtgcg aagcggtttg
     1921 ggacacctgg tgattgcaga tcatgatact gtttcccggg aaaacctgga ccggcaattc
     1981 ttttttgcag accaggaggg acgtcccaaa gtctatgctt tgcaggataa tctgcagcgc
     2041 ataagttccg gtaccaggct taccttgttt tacggaagtg tgaccgcccg caatgccaga
     2101 gagctgtttt cgtgttgtca tgttgtggtt gaagcctttg acatggcggc tgaaaaagaa
     2161 tggtttgcta cctggatgac agaaaacctt tcccacatcc cccttgtcat gggttcgggc
     2221 atagccgggt ggggtcattc ggataagctc cgggtgcagc gttccggtaa tttgtatgtt
     2281 tgcggacatc agctggatac ccatgaaacg gaagtgcccc tgatggcccc tcgtgttggc
     2341 ctggttgcca tgatgcaggc agatattgta ctggagatct tattgacaac aaaagcacca
     2401 tcccaatgac catcaccttg aacaacagaa aagaaacgtt ccctggcggt cccatgacca
     2461 ttgcccgggt catggaagaa aaatccttcc atttcaaaat gctgatagta aagctgaatg
     2521 gaaacatcat atccaaagaa gcgtttgcct caaccctggt agaaaacggt gaccgtctgg
     2581 atatcatcca tctcatcagc gggggctgaa gatgacatat cacgaaaatt tatgaaaaca
     2641 accttatttt accaccttgc aggactgata ctgatagcaa cggctttcct tgccccttcg
     2701 tgcatttccc ttgatctgga cgcgatcagc ggatctaccc
//
LOCUS       DAOSOF010000192         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_37704_length_2702_cov_4.261428, whole genome shotgun sequence.
ACCESSION   DAOSOF010000192 DAOSOF010000000
VERSION     DAOSOF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37704_length_2702_cov_4.261428"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLM68_08475"
     CDS             complement(<1..1065)
                     /locus_tag="PLM68_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOT55427.1"
                     /translation="MMKKTKTILTGLFLLFCSMLMAQNIQVTGKVTDAVDGQPLPGAT
                     IFVQGERTAQVTDNQGNYVISCPPDATLIFSYVGYLSVEVLVNNRSVINVEMRDVNVL
                     EEVIVTALGITRERKSIGAAIQDVKAEEITKSGHLNLSSALSGRVAGMQVTQAGGAIG
                     ASSRIVVRGNSSLSGNEPMIVVDGIPISNDNYMQNAVDYGSGLTDINPEDIENIAVLK
                     GGSAALYGMRAGNGVVLITTKSGKAAREGLSITYNGSFTVDNVYNLPPLQNLYGQGYE
                     GAEYEFNRSGYNGTYANWVYDELGYSDLASYFGADESWGPRLDIGLNIPQWDSPYLND
                     SHQATPWVSHPDNIKSFFNTG"
     gene            1379..>2702
                     /locus_tag="PLM68_08480"
     CDS             1379..>2702
                     /locus_tag="PLM68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT55428.1"
                     /translation="MKKFLLLTLSVIMGFTALFAQQRVTGTVTSSEDGAPIPYVTVVV
                     AGTTITSQTNLDGVYSINVPAGSNTLRFTFVGMQTVVADINGRAVVDVIMYPDAIALE
                     DVVVIAYGTAKKESLTGSATVVDSQKLSRRVVSNVTKALDGLSTGVITTSGSGQPGSG
                     ASVIIRGYGSINASQTPLYVVDGIPFNGNLNAINPADIESMTVLKDASSGALYGARGA
                     NGVIMITTKKATSEKVSITYNGSAGISNRALKRYDMVGQRDFVTMTYESLRNAYAFDN
                     GQPWEVASQNAMNDLSNSLGGELYNPFKNYTWATVIDPATGAVREDAVSAWDENWMDE
                     IEQQNAFRHEHQLTVAGGSARTKALMSLGFLDENGILKTTSFQRFSGRANVDSQVNDW
                     VKAGLNLSASMTKSNSSGYTGSAYANVWYSAQFMAPIYPVWLKDLSGQD"
BASE COUNT          725 a          645 c          639 g          693 t
ORIGIN      
        1 gccggtatta aagaagcttt taatgttatc tggatgcgaa acccaggggg ttgcctggtg
       61 tgaatcgtta agatacgggc tgtcccattg ggggatgttc aggcctatat caaggcgtgg
      121 accccagctt tcgtcagctc caaaataact ggccagatcg ctgtaaccaa gttcatcgta
      181 aacccagttc gcgtaagttc cgttgtatcc gcttctattg aattcgtact cggcaccttc
      241 gtatccctgt ccgtacaggt tctgaagcgg gggcaggttg taaacgttgt ctaccgtaaa
      301 gctgccgttg tacgtgatgg agaggccttc tctggcagct ttcccggatt ttgtggtaat
      361 cagtacaacg ccattgccgg cccgcatacc atacaaggca gccgatccgc ccttgagtac
      421 tgcaatgttc tcgatatctt cgggattgat atctgtcaat cccgatccat agtccacggc
      481 gttctgcatg tagttgtcgt tggaaatggg tataccatcc acaacaatca tgggctcatt
      541 gccgctcaag gaagagttac cccttacaac aatacggctt gaggccccaa tggcaccacc
      601 agcctgggta acctgcatac ctgccacacg ccctgacagc gcagaagaaa ggttcaggtg
      661 cccggatttg gtgatctcct cggctttcac atcctggata gccgccccta tcgattttct
      721 ctcacgggta ataccaagtg ccgtaacaat cacttcttcc agaacgttga catcacgcat
      781 ctcaacgtta ataacagaac gattgtttac tagaacttct acactcaggt accccacgta
      841 actaaagata agggttgcat ccggaggaca tgaaatgaca tagttaccct ggttatcggt
      901 aacttgagca gtgcgttctc cttgtacaaa gatggttgca cctggaagcg gttgtccgtc
      961 aaccgcgtcc gtaaccttgc ctgtaacctg gatgttctgg gccatcagca tggaacagaa
     1021 cagcaggaac aagccggtca ggatagtttt ggtttttttc atcatgaatg aaattaggtt
     1081 aaagaattag gtaaaaagct atcaaatata aatatttgaa atgaaacggg catgaaacag
     1141 ttttttgaaa gataaacagt aactattcag gtgaaatttc aaaaataatt gctgtttttt
     1201 gcttaaaatt atttcatttt gtaagaaaga ttctgttgag acgcggggat ttgtatcaaa
     1261 aaatcaacga cagggagaaa aaaacgcttt tatgtttgaa aaattcatta tctttgtaaa
     1321 ctacgcggca cattaagata aaccctataa actatttact aacctaaaaa aaagacagat
     1381 gaaaaaattc cttttattga ctttgtcagt cataatgggg ttcacagcat tgtttgccca
     1441 acagagggtt accgggacgg tgacatcctc ggaagatggt gctccgattc cctatgtgac
     1501 agtggttgta gcgggaacaa caattacctc gcaaaccaat ctggatggtg tctactctat
     1561 taacgttccg gctggcagca atacccttcg tttcactttc gtgggtatgc agacggtcgt
     1621 tgcggacatc aatggcagag cggttgttga tgttatcatg tatcccgatg ctattgcact
     1681 ggaagacgtt gttgtgatcg cctatggtac agcaaagaaa gaatccctga caggttctgc
     1741 aactgttgtt gacagccaga aattgtcaag acgcgttgtt tctaacgtaa ccaaggcttt
     1801 ggatggtctt tccaccggtg ttatcaccac gtccggttcc ggacagcccg gctcgggagc
     1861 ctcggtgatc atccgcggat acggatctat caatgcaagc cagaccccat tgtatgttgt
     1921 ggacggaatc cctttcaatg gaaatctcaa tgccatcaac cctgctgaca ttgaaagcat
     1981 gaccgttttg aaagatgctt cctccggagc actgtacggt gcacgtggcg ccaatggtgt
     2041 tatcatgatc accaccaaaa aggcaacatc agaaaaagta agtatcacct ataacggcag
     2101 cgccggtata tccaaccgtg ccctcaaacg ttatgatatg gtaggccaaa gggacttcgt
     2161 gaccatgact tatgaatcgt tgcgcaacgc atatgcattc gacaacggtc agccgtggga
     2221 agttgcctca cagaacgcca tgaatgacct gtcgaattcc ctggggggag agctttacaa
     2281 tcccttcaag aattacactt gggcaaccgt catcgatccg gctaccggag cggtaaggga
     2341 agatgccgtg tcagcatggg atgaaaactg gatggacgag atcgaacaac agaatgcatt
     2401 ccgccacgaa caccagttaa cggttgccgg tggcagcgcc aggaccaaag ccctgatgtc
     2461 actcggtttc ctggatgaaa acggtattct gaaaaccact tctttccagc gcttttccgg
     2521 tcgtgccaac gtggacagtc aggtgaacga ctgggtaaag gcaggcttga acctgtctgc
     2581 atccatgaca aaatccaact catcgggtta cacaggatct gcctacgcca acgtttggta
     2641 cagcgcccag ttcatggccc ccatttaccc tgtatggctt aaagacttaa gtggtcagga
     2701 cc
//
LOCUS       DAOSOF010000193         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_37746_length_2700_cov_6.286957, whole genome shotgun sequence.
ACCESSION   DAOSOF010000193 DAOSOF010000000
VERSION     DAOSOF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37746_length_2700_cov_6.286957"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2651)
                     /gene="secDF"
                     /locus_tag="PLM68_08485"
     CDS             complement(<1..2651)
                     /gene="secDF"
                     /locus_tag="PLM68_08485"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOT55429.1"
                     /translation="MQSKGAIWLVAILITVACLYQLSFTAVTQIQENKAAGYAEKAIV
                     EIQKTPEYQNISEFDQAFFLDSLRNAKESYYIDSITPEKVYLWYTYKEVKEREISLGL
                     DLRGGMNVMMEVKLENLIRALTDYSQDPIFNQAIELTNQKVKEGARGNYVDLFAASWE
                     EIAPGERLSRIFGTYDMRERIKPETSNEEILRIISTEAESAMANSFNVLRNRIDRFGV
                     TQPNIQRLGTTGRILVELPGIKEPERVRKLLQGTASLEFWETYENSEVYPALEEANRI
                     LRDYLDDMKPAATEEDSLTAEAGEENDLLAQLAKEDSLSEDLTAWAQQNPLFNVLNPS
                     VYEGQLAPGPCVGRAHYRDTAMINTWLNLPQVQAALPADVRLLWSVKPDKVNQVYYEL
                     VAIKANTRDGRAPLDGGVVTDANKQFDQTGGGFPSVDMGMNAEGARIWARMTADNIGK
                     CIAIVLDDMVYSYPRVSVEITGGRSSITGDFTSDEADDLANVLKSGKLPAPTTIVQES
                     IVGPSLGTRSINAGMGSFLIAFLLVLAYMIFFYNRSGVYASVALLTNLLFLFGALVSF
                     GAVLTLPGIAGIVLTLGMAVDANVIINERIKEELRTGKAIRLAIADGYKNAYSAILDG
                     NITTMITGIVLIFFGSGPVKGFATTLVIGLVTSLFTSIFITRLLIERRLTKNKPISFD
                     NRITRNFLTNTKIKFLEYRKYYYIAAIAISLIGLVFMFTKGFTYGVDFTGGRTYVLRF
                     DQEVSSEAVREKVTEAFQGGVEVKEFGGKSQMRVTTRYMIQDESAEADAMVDEMLYNA
                     VKEFFVTPLTLEDFLSTLENPNGVISSEKVGPTVASDIQRDAAIAILLSCVAIFLYIA
                     ARFRNWSWGIGGLISLIFNTLFVVSFFS"
BASE COUNT          669 a          680 c          636 g          715 t
ORIGIN      
        1 gagaagaaag aaacaacaaa aagcgtattg aaaatcaggg atataagccc acctattccc
       61 caggaccagt ttctgaaacg ggcggcaata taaaggaaaa tggccacgca ggagagcagg
      121 atggctatcg cggcatcacg ctgtatatca ctggccactg tcggccccac cttctccgaa
      181 ctgatcactc cgttgggatt ttccagcgtg gaaagaaaat cttcaagtgt cagcggggtc
      241 acaaagaact ccttgaccgc attgtaaagc atttcatcca ccatcgcatc ggcttctgca
      301 ctttcatcct ggatcatata tctagtagta acgcgcatct ggctttttcc gccgaattcc
      361 ttgacttcta ccccaccctg gaaagcctcc gtaacttttt cacgtacggc ctcggaggag
      421 acttcctggt caaagcgtaa gacatacgtt cttcctccgg tgaaatcaac cccgtatgta
      481 aagccctttg tgaacatgaa gacaagtcca atgagggaaa tggcaatggc tgcgatatag
      541 taatatttcc tgtattccag gaatttgatc tttgtgtttg taaggaaatt acgagtaatc
      601 cgattatcaa aggaaatggg tttattcttg gtcagtcgtc tttctatcaa aagccttgtt
      661 atgaaaatgg acgtgaacag ggatgtaaca agacctataa ccagggttgt ggcaaatccc
      721 ttcacgggcc ctgaaccgaa gaatataagg actatcccgg ttatcatggt ggtgatgtta
      781 ccgtccagaa tggccgaata agcattttta taaccgtctg cgatcgcaag acgtatcgct
      841 ttacccgtcc gcaactcttc tttgatgcgt tcgttaatga tcacgttggc atccactgcc
      901 atacccaagg tcagtacgat accggcaatc ccgggcaggg tcagtacggc tccgaaggaa
      961 acaagcgctc cgaagagaaa cagcaggttg gtcagcaatg ccaccgaagc gtataccccg
     1021 gaacgattgt agaagaaaat catgtaggca agcaccagca gaaaggcaat tagaaatgac
     1081 cccatacctg catttatgga acgtgtccct agtgacggcc ccacaatgct ttcctgtaca
     1141 atggtggttg gagcaggcag tttaccggat ttcaacacgt tggcaaggtc atccgcctca
     1201 tcggaagtaa aatcgccggt aatggatgaa cggccacctg tgatctcgac gctgaccctg
     1261 ggataggaat agaccatatc atccaggacg atggcaatgc atttgccaat gttgtcggct
     1321 gtcattcttg cccaaatacg ggcaccttct gcattcatcc ccatgtctac agaggggaat
     1381 ccgccgccgg tctgatcaaa ttgtttgttt gcatcggtta ccacacctcc gtccaggggt
     1441 gcccgtccgt ctcgtgtatt tgccttaatg gcaaccaatt cgtaataaac ctggttaacc
     1501 ttatcgggtt tcacagacca cagaaggcgt acatctgcag gcagggctgc ctgtacctgg
     1561 ggaagattca gccaggtgtt gatcatggcc gtatcgcggt aatgcgcccg gcccacacag
     1621 ggtcccggtg cgagctggcc ttcataaaca ctggggttga gaacgttaaa cagtggattt
     1681 tgttgtgccc aggctgtgag atcttcggaa agagaatctt ctttggccag ctgtgccagc
     1741 aggtcgtttt cctccccggc ttccgccgtc aatgaatctt cttctgttgc tgccggtttc
     1801 atatcatcca ggtaatccct caggatccgg tttgcttctt caagtgccgg gtatacctcg
     1861 gaattttcgt aggtttccca aaactcaagg gaggctgtcc cctgcagcag tttgcggaca
     1921 cgttccggtt ccttgatgcc gggcagctct accaggatgc gtccggtcgt ccccaaacgc
     1981 tgaatattag gctgggtaac accaaaacgg tcaatacggt tgcgcagcac attgaatgag
     2041 ttggccatgg cagactcggc ttccgtgcta atgatgcgga gaatctcttc attggaagtc
     2101 tcgggtttga tgcgctcccg catgtcataa gtcccgaaaa tcctggagag cctttctccc
     2161 ggtgcgattt cttcccatga ggcggcaaac aggtccacat aattgcccct tgccccttcc
     2221 ttcacttttt ggttggtcag ttcaatagcc tgattaaaaa tagggtcctg gctataatcg
     2281 gtcagtgccc ttatcaggtt ttcgagcttt acttccatca ttacgttcat cccgcccctg
     2341 aggtcaagac ccaggctgat ctccctctcc tttacctcct tgtaggtata ccacaggtat
     2401 actttttctg gggttatcga gtcaatgtaa tagctttctt tagcgttccg cagcgaatcc
     2461 aggaaaaaag cctggtcaaa ctcgctgata ttttgatatt ccggggtttt ctgaatttca
     2521 acaatggctt tttctgcata cccggccgct ttgttttctt gtatttgggt tactgcagta
     2581 aaagacaact gatacagaca agctactgta atgagtatcg ccaccagcca gatggcacct
     2641 ttactttgca taattttatt attaatttat aagtcaggta aaatagggtg caaatgtaat
//
LOCUS       DAOSOF010000194         2695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_37838_length_2695_cov_4.608333, whole genome shotgun sequence.
ACCESSION   DAOSOF010000194 DAOSOF010000000
VERSION     DAOSOF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37838_length_2695_cov_4.608333"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLM68_08490"
     CDS             complement(<1..672)
                     /locus_tag="PLM68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT55430.1"
                     /translation="MKVLMFGWEFPPHISGGLGTACDGLTRGLAKHNVQVLFVMPSAS
                     GDEDQSAVRIINASDVAAFETREEARQLFEKVQFLKVTSNLIPYLGPDEFEEKVDLER
                     EKEWNEFRISFGQKFKFSGKYGKNLMEEVMRYALVASAIAHKNQFDVIHAHDWLTYMA
                     GIAAKRVSGKPLVVHVHATEYDRSGENINTQVYDLERRGMEAADRVIAVSNLTRNTII
                     TRYHID"
     gene            complement(669..2579)
                     /locus_tag="PLM68_08495"
     CDS             complement(669..2579)
                     /locus_tag="PLM68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOT55431.1"
                     /translation="MAYLQFNKHELGNLEYSLNREILSTNRAGGYLNTTLPGCNTRKY
                     HGLMVVPVDRLGGENYVLLSSLDETLIQHDQAFNLGIHRYPGIYEPRGHKYIIDLEMD
                     KIIVLTYRIGGMIFKKEIVFLHNKEQLLIRYTLIEAHSPTTLRLKPFLAFRKIHDLTF
                     ANLAADTFHSTVEKGVGYCLYENFPRLYMQLSVANEFVKYPDWYKNIEYLREKDRGYD
                     YHEDLFVPGYFECPIHKGRSIVFSASLVPVAPSGLQAVFNRELANRPSRDSFSNCLKL
                     SASQFIITRNGKTQVFAGYPWSRRCPRITAIALPGLALSTENSLKIFTQALETLVGDE
                     QSLYHAADTPLWLFWTLQQYLGQTGDRKALWRKYGHVMKSIVRNYKTGKDRVTMHANG
                     LIWCGEGDVPMTWMDSVVNGKPVTPRSGYIVEICALWYNAVCFALDLAKDMEDNEFVK
                     EFEDIPGLVKENFLPTFWCESRSHLADYVNDQGQNIFTRPNQLFACSLPYTPLEDEVI
                     NSVLRACSGELLTTRGLRTLAPKNPLFKSRCEGDADQRHRAYHQGSVWPWLLGHYIDA
                     QFRLHGRGFVDCARDLIGVFEEDMTEHGIGSIAELYDGNPPYRPHGCTSSALSVAEIL
                     RVMNLIDKYNTL"
BASE COUNT          638 a          697 c          658 g          702 t
ORIGIN      
        1 gtcaatatgg taacgtgtta ttatggtatt gcgtgtgagg ttgctcacgg ctatgacacg
       61 gtcggcggct tccatccccc gcctttccag gtcatagacc tgggtgttga tattctctcc
      121 tgaacggtca tattctgtgg catgaacatg caccacgagc ggttttcccg aaacgcgttt
      181 tgcagcaatc cctgccatgt aggtgagcca gtcatgggcg tgtatgacat caaactggtt
      241 cttatgggca atggccgatg ccacaagcgc atagcgcatc acttcctcca tcaggttctt
      301 gccgtatttt ccggaaaatt tgaatttttg tccgaatgat atcctgaatt cattccattc
      361 cttctccctt tccaggtcca ccttttcttc aaattcatcg gggcccaggt aagggatcag
      421 gtttgacgtc actttcagga actgcacctt ctcaaaaagc tgcctggcct cttccctggt
      481 ttcaaaagcc gctacatcac tggcattgat gatgcggaca gcactctgat cctcatcacc
      541 cgaagccgag ggcatcacaa acaggacctg gacgttgtgt tttgccagcc ccctggtcag
      601 gccgtcgcag gccgtgccca gtccgccgga tatatgagga ggaaattccc atccaaacat
      661 taatactttc atagtgtatt gtatttatct atgagattca tcacacgcaa aatctcggct
      721 acgctcaggg cgctggaggt gcatccgtga ggacggtacg ggggattgcc gtcgtatagt
      781 tcagcaatgg atccaatccc gtgttcggtc atatcttctt caaaaactcc aatgaggtcg
      841 cgggcgcaat caacaaatcc cctgccatgc aaacggaact gggcatcaat ataatgaccc
      901 agcagccatg gccatacgga gccctgatgg tatgccctgt ggcgttggtc ggcgtctcct
      961 tcacaacggc ttttaaacaa cggattcttt ggggcaagcg ttctcaggcc ccgggttgtg
     1021 agcagttcgc cgctacaggc ccttagaacg gaattgatca cctcatcctc caggggcgta
     1081 tagggaagcg aacatgcaaa caattggtta ggtcttgtga aaatgttctg tccctggtca
     1141 ttgacgtaat cggccaggtg agaacgggat tcacaccaga aagtgggcag gaagttttcc
     1201 ttgacaagac cgggaatatc ctcgaactcc ttcacgaatt cattgtcttc catatccttg
     1261 gccagatcca gggcaaagca gacagcattg taccacaggg cgcagatttc cacaatatag
     1321 ccgctgcgag gagtgaccgg tttcccgttc accaccgaat ccatccatgt catgggaaca
     1381 tctccctctc cgcaccatat cagcccgttg gcgtgcattg tcacccggtc tttgcccgtt
     1441 ttgtaattcc ttacaatgga tttcataacg tgaccgtatt tacgccatag tgctttcctg
     1501 tcgcctgtct ggccgaggta ttgctgcagc gtccagaaca gccacagggg ggtatcggct
     1561 gcatggtaaa gactctgttc gtctccgaca agcgtttcca gcgcctgtgt gaaaattttc
     1621 aaactattct cagtagacag ggcaagtccc ggcaaggcaa tggccgtaat ccttggacac
     1681 cgcctggacc atggatatcc ggcaaacacc tgtgtcttgc cgttcctggt tatgatgaac
     1741 tgtgaagcag aaagcttcag gcaattactg aaggaatccc ggctgggtct gttggccagc
     1801 tcccggttaa agacagcttg caggccggaa ggggcaaccg gtaccagtga tgccgaaaag
     1861 acaatgctcc gtcctttatg tatggggcat tcaaaatatc cgggaacaaa caggtcctcg
     1921 tggtaatcat agcctctgtc cttttccctg aggtattcta tgtttttata ccagtcgggg
     1981 tatttgacaa actcgttcgc cacactgagc tgcatgtata accggggaaa attctcgtag
     2041 aggcagtagc ccacaccttt ttccacggtg gaatggaaag tatctgccgc caggttggca
     2101 aaagtcaggt catgaatctt gcggaaagcc aggaatggtt tcagacgcaa tgttgtgggc
     2161 gaatgggcct ctatcagggt atacctgatg agcaactgtt ctttattatg caggaaaacg
     2221 atttcctttt tgaagatcat gcccccgatc ctgtatgtca ggacaatgat cttgtccatt
     2281 tcaaggtcga tgatgtactt atggcccctc ggttcataga tccccggata acggtgaata
     2341 cccaggttga aagcctggtc gtgctgtatc aatgtttcat ccagtgaaga caacagcaca
     2401 taattttccc ctcccagcct gtcaacaggg accaccatga gtccatgata ttttcttgtg
     2461 ttgcaacccg gcagggtcgt gttcagatag ccgccagccc tgttcgtaga aagaatttcc
     2521 ctgttcagtg aatactccag gtttcccagc tcgtgcttgt tgaattgtaa atatgccata
     2581 atcttacctt aaagcttaag cttgcaagtt actaattaat cctcaattaa caccatggcg
     2641 gtacgtgccg gcaggtaaag gaacaggaca ttggtacccc gctgcggaag ggtga
//
LOCUS       DAOSOF010000195         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_38236_length_2672_cov_5.291173, whole genome shotgun sequence.
ACCESSION   DAOSOF010000195 DAOSOF010000000
VERSION     DAOSOF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38236_length_2672_cov_5.291173"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLM68_08500"
     CDS             <1..564
                     /locus_tag="PLM68_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOT55432.1"
                     /translation="SGHIPTPGHRSTEKMVQWENYVFVTCWSYDNKILVIDTATDRIT
                     DSLEVGHQPTDLVLDRNGKIWCAAAGNLYRIDARSRNIEKIFPLRADGARTHLALNAF
                     SDTLYYLNKSLWRMAINQATTGNTPFIVLDDRPYYGLAVDPVSSDIYVADALDYVQPG
                     KVYRFNPGGEPVDTLTTGINPGSFCFK"
     gene            567..2024
                     /locus_tag="PLM68_08505"
     CDS             567..2024
                     /locus_tag="PLM68_08505"
                     /inference="COORDINATES: protein motif:HMM:NF028130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD-like domain-containing protein"
                     /protein_id="HOT55433.1"
                     /translation="MNRKHNLLLCISLFLLAGCNMGRELYNEPAPLITFDVPDGQYTV
                     KTGQTLAIMPQISYDTGASYTWELDGITVGTDKYFTFSAQEPGTYFLTFKVTRPDITV
                     SKQVRINVIRKAHPHIFLAVPVDGYSVLTGTSLTIRPIVENNENTEYLWKVNQVEVSR
                     DSIYTFTSSYEGNYDVFFSAVNEDGADEILFRVNVADTLPGSGDTGTTDDPPLPSDKY
                     YRPSNQESHAHWSAVLEYTPAPGQFINELETGGFSGENTREGSIRYAEKRLKEGLFVS
                     LGAFGGYLVAGFDHSIVNDGGYNLQITGNAYENSSEPGIVWVMQDSNGNGLPDDTWYE
                     LKGSEHSNSSTRRNYAVTYFRPREPESPVHWIDNEGNTGTIDYLGSFHSQDSYYPLWI
                     DRDYYTLTGTCLESCNYLSQSGSWISPPYGWGYADNNDQDLFRIADAVTPKGDPVEMG
                     FIDFVKVQTGVQGKSAWLGEISTEICSISDYNLIK"
     gene            2021..>2672
                     /locus_tag="PLM68_08510"
     CDS             2021..>2672
                     /locus_tag="PLM68_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HOT55434.1"
                     /translation="MRRFLIPAFMMLLSGFTTHAQDSVEVRQLDSIRITARARLRDMG
                     VQKTSLDSLELRNDITGSLAQVLTRTSTLFIKHYGRATLSTASFRGTSPSHTQVTWNG
                     MKINSPMLGMVDFSTIPSYFVDKADLYHGPGSVTISSGGLGGAIALSSAIPQQDGISL
                     SFIQGAGSFLTFDEFLHFTVKKPTWSASTRVYFVSSRNDYKYINYRKKHFFYDDQGN"
BASE COUNT          710 a          675 c          676 g          611 t
ORIGIN      
        1 tcgggtcata ttcccacccc cgggcaccgt tccacggaaa aaatggtcca atgggaaaat
       61 tatgtatttg taacctgctg gtcttatgat aataaaatac tggtcatcga taccgctaca
      121 gaccggataa cagattcgct ggaggtgggt caccagccga ctgacctggt gctggacagg
      181 aacggaaaaa tctggtgtgc cgcagccggg aatctttacc gcatagatgc ccgcagcagg
      241 aatattgaaa agatcttccc gctccgggct gacggagccc gtacccatct tgccctcaac
      301 gcgttctccg acacactgta ttacctgaat aaaagcctgt ggcgtatggc cataaaccag
      361 gctactacag gcaatacgcc attcatagtg ctggatgaca ggccatatta tggtctggcc
      421 gttgatccgg tatcatccga tatatacgta gccgatgccc tggactatgt ccagccagga
      481 aaagtctacc gtttcaatcc cgggggagaa ccggtggaca cacttaccac tggaatcaac
      541 cccggatctt tttgtttcaa atgaacatga acagaaaaca taacctgctg ctgtgcatta
      601 gcctgtttct cctcgccggg tgcaacatgg gaagggaatt gtacaatgag cctgcccctc
      661 tgataacgtt tgatgtccct gacggtcaat acacggtgaa aacaggacaa accctggcta
      721 tcatgccgca gatcagttat gataccgggg cctcctacac atgggaactt gacggaataa
      781 cagtcggaac agataaatat tttactttca gtgcacagga gcccggaact tacttcctga
      841 cctttaaggt gacaagacct gacataaccg taagcaaaca ggtacggata aatgtcatca
      901 ggaaagctca cccgcatatc ttcctggccg tccccgttga cggctattcc gtccttacgg
      961 gcacctctct tacaataaga cccatagtgg aaaacaatga aaatacagaa tacctgtgga
     1021 aagttaacca ggtggaagtg agccgggaca gcatctatac cttcacatca tcctatgagg
     1081 gaaattatga tgtgtttttc agtgctgtca atgaagacgg agcggatgaa atcctgttca
     1141 gggtcaatgt ggcggatacc ttgcccggaa gtggtgatac cggcactacg gatgatccgc
     1201 ccctgccctc ggacaagtat taccgtcctt ccaaccagga aagccatgca cactggagtg
     1261 cggtcctgga atacaccccg gctcctggac aattcatcaa cgagctggaa acgggaggat
     1321 tttcgggaga gaacacgcgc gaaggatcca tacggtatgc agaaaagcgg ttgaaagaag
     1381 gtttattcgt atcccttggg gcttttgggg gatacctggt cgcgggtttc gatcacagta
     1441 tagtgaatga tggagggtac aacctgcaga taacgggaaa cgcatacgag aactcctcgg
     1501 aaccaggaat cgtatgggtg atgcaggaca gcaacggaaa cggcctgccg gatgatacct
     1561 ggtatgagct taagggaagc gaacacagca atagcagcac caggaggaat tatgcagtca
     1621 cgtatttccg tccccgcgaa ccggaaagcc cggtacactg gatcgataat gaaggtaata
     1681 ccgggaccat agattacctg gggtcgttcc acagccagga cagttactat ccactgtgga
     1741 ttgacaggga ttattacacc cttacgggca cgtgtcttga gtcatgcaat tacctttccc
     1801 aaagcggttc atggatcagt cctccatacg gctggggcta tgcagacaac aacgaccagg
     1861 atttgttccg catagcagat gccgtaaccc ctaagggaga cccggttgaa atgggtttca
     1921 ttgattttgt caaagtgcag accggggtgc aggggaaatc agcatggctg ggagaaatat
     1981 caaccgagat ttgctctatc agtgattata atcttatcaa atgaggaggt ttttgatccc
     2041 ggccttcatg atgctcctgt ccggtttcac tacccatgcg caggattcgg ttgaagtgcg
     2101 ccaactggat tccatccgca taacagcccg ggcaaggctc cgggacatgg gggtgcaaaa
     2161 gacctctctg gattcattgg agctgcgcaa tgatatcaca ggatccctgg cacaggtact
     2221 gacgcggacc agtacgctgt tcattaagca ctacggacgg gccaccctgt ccaccgcatc
     2281 tttcaggggc acctccccgt cccatacaca ggtcacatgg aatgggatga agatcaattc
     2341 tcccatgctg gggatggtgg atttttcaac cattccctcc tatttcgttg acaaggccga
     2401 tctttatcat gggcccgggt cagtgacaat atcctcgggc ggactgggcg gagctatagc
     2461 cctttccagt gcgattccgc aacaggatgg gatttccctc agtttcatcc agggagcggg
     2521 cagcttcctg acttttgatg agttcctgca cttcaccgtc aaaaaaccaa catggagcgc
     2581 ctcgacccgg gtctattttg tatcctccag aaatgactac aaatacataa actatagaaa
     2641 aaaacatttc ttttacgatg accagggcaa tg
//
LOCUS       DAOSOF010000196         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_39203_length_2619_cov_4.934477, whole genome shotgun sequence.
ACCESSION   DAOSOF010000196 DAOSOF010000000
VERSION     DAOSOF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39203_length_2619_cov_4.934477"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLM68_08515"
     CDS             complement(<1..553)
                     /locus_tag="PLM68_08515"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT55435.1"
                     /translation="MDKGKRNGNIYTIVYAALMVTLVAALLAFASQLLKPRQVANMMA
                     ETQQTILRSVHLEDQPYEKYITDTVAGPDQLPLFICTLDNGTRKYIIPLHGKGLWGPL
                     WGYLALDDDFSTIYGAVFDHISETPGLGAEITTLFFSDPFRGKSLYHGDQFVSISVLK
                     QGRSQNNPNAVDGISGGTLTSRGV"
     gene            complement(546..1664)
                     /locus_tag="PLM68_08520"
     CDS             complement(546..1664)
                     /locus_tag="PLM68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOT55436.1"
                     /translation="MKWIENIIEKIKPAFSKGGRLAFLHSTFNAFETFLMVPGTVTGK
                     GSHIRDCNDLKRVMIVVVIALIPCLLFGMYNTGLQHYLAMDPRAELPGFFPVFWYGFL
                     KVLPLVIVSYVVGLGIEFTIAQIRGHEVNEGYLVTGMLIPLIVPVTIPLWMLALAVAF
                     AVLIGKEIFGGTGMNIWNPALLARAFLFFSYPAYMTGGKVWVVDGTSGATPLAALAEG
                     TPLQELPSWTDMFLGFIPGSVGETSLVCILLGAAILLYTGVASWKIMISCVAGAVLIA
                     LTGDIPVHYHLVMGGFAFGCVFMATDPVTSPQTETGKWIFGFLTGALTITIRLYNPGY
                     PEGMMLAILLMNTFSPLIDHVVVSVNVKRRIKRIARHG"
     gene            complement(1661..>2619)
                     /locus_tag="PLM68_08525"
     CDS             complement(1661..>2619)
                     /locus_tag="PLM68_08525"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOT55437.1"
                     /translation="GITALPGETPKAIYISCFDSAPLAPHYDFTFAESFESLQMGLRV
                     LERLCPGKVHLGLRDTTSPHSVFRRLPGIAHIFSGPHPAGNPGVQLHHVCPVGKDQIA
                     WTLTPMGVSIIGKLFLYGIYDTTRMVAVTGPALSRTGYITCHAGFQVGALNPFPLKEG
                     TDARIISGNPLTGDTIGREGFLGYHHQQVTCLPEGGKRELLGWARPLRLKKFSAARTY
                     ISYLAPLLGKTGRFNLDTNVNGGARAFVLTRNYQKVLPMDIFVSHLIKAILAEDIDKM
                     EQLGIYEVIPEDLALCEFVCPSKIEIQEILQNGIDLMIKEMQ"
BASE COUNT          633 a          675 c          721 g          590 t
ORIGIN      
        1 caacaccacg actggtgagc gttccgcctg aaataccgtc aacagcattg ggattatttt
       61 gggaccgccc ctgctttagc accgatatcg agacaaactg atccccgtgg taaagggatt
      121 tgccccggaa cggatcgctg aagaaaaggg tggtaatctc ggccccaagc ccgggcgtct
      181 cggaaatgtg gtcaaagacc gccccgtaga tagtactgaa atcatcgtcc agggccaggt
      241 atccccataa ggggccccag aggccttttc catgcagggg aatgatgtat ttgcgggtgc
      301 cgttgtccag tgtgcaaatg aaaagaggca attgatccgg tcctgccacg gtgtcggtta
      361 tgtatttctc atagggttga tcctcaaggt gtactgaccg gaggatggtt tgctgtgttt
      421 ccgccatcat attggctacc tgcctgggtt tgagcagctg tgaagcgaaa gccagcaggg
      481 cggccaccag ggttaccata agggcggcat acacgatggt gtaaatgttc ccgtttcttt
      541 tccctttatc catggcgtgc aattctcttg attctgcgct ttacattgac cgaaaccaca
      601 acgtggtcaa tcagcggaga aaaagtgttc ataagtagta tggcgagcat cattccctcg
      661 gggtatccgg gattgtagag ccggatggta atggttagtg ctccggtcaa aaatccgaag
      721 atccattttc cggtctcggt ctgggggctg gtaacaggat cggtggccat aaagacgcac
      781 ccaaaggcaa agcctcccat caccaggtgg taatgcacgg ggatgtcccc cgttaaggct
      841 atcagaacgg ctcccgccac acaggaaatc atgattttcc agctggccac cccggtgtaa
      901 agcaggatgg ctgcccccag caggatgcac accagggagg tttcccctac agaaccggga
      961 ataaagccca ggaacatatc cgtccaggaa ggcagttcct gcagcggagt cccttcggcc
     1021 agggcagcca gcggtgtggc cccgctggtg ccgtcaacca cccagacttt gcctcctgtc
     1081 atgtatgcgg gataggagaa aaaaaggaaa gcacgcgcca gcagggcggg attccagatg
     1141 ttcattccag ttcctccgaa aatctctttt ccgattagca ccgcaaaagc tacggccagg
     1201 gcaagcatcc acaacgggat ggtcaccggc actatcaggg gtatgagcat tcccgtgacc
     1261 aggtatcctt cgttaacctc gtggccccgt atctgtgcta tggtgaattc tatgcccagg
     1321 ccgaccacat aagatacgat caccagaggc agtactttca gaaaaccata ccagaaaacg
     1381 gggaaaaagc cgggcagttc tgcccgaggg tccatggcca gataatgttg cagccccgtg
     1441 ttgtacatcc caaaaagcag gcaggggatc agggctatca caactacaat catcacgcgc
     1501 tttaggtcgt tgcagtcgcg tatatgagat cctttgccgg taacggtgcc gggaaccatc
     1561 aggaacgttt caaaggcatt gaatgtggaa tgcagaaacg ccaggcggcc acctttgctg
     1621 aaggcaggct ttatcttttc tatgatattt tcaatccact tcattgcatt tcctttatca
     1681 tcagatctat cccattttga agaatttcct ggatctcgat cttggacggg cagacgaatt
     1741 cgcacagggc aaggtcttca ggaataactt catagatccc caattgctcc attttgtcaa
     1801 tgtcctctgc cagaatggct ttgatcaaat gcgagacaaa gatatccatg ggcaggactt
     1861 tctggtaatt cctcgtgagg acaaaggccc ttgcgccacc gtttacattg gtatccagat
     1921 taaagcggcc ggttttaccc agtagggggg ccaggtagga tatataggtc cgtgctgcgc
     1981 tgaatttttt caggcgcaac ggtctggccc atcccagcag ctcccttttc ccgccttccg
     2041 gcaggcaggt gacctgctgg tgatgatatc ccaggaaacc ttccctgcca atagtgtcac
     2101 cggtaagggg attgccgctg atgatgcgtg catctgttcc ttctttaagg gggaacgggt
     2161 tgagagcacc gacctggaaa ccggcatggc aggtgatata acccgttctg ctaagggcag
     2221 gaccggttac ggcaaccatc cgggttgtat cgtagatacc gtataggaag agcttcccaa
     2281 tgatcgacac acccatcggg gtgagcgtcc aggcaatctg atcctttcca acaggacaaa
     2341 cgtggtgcag ctgtaccccg ggattcccgg cgggatgggg tcctgaaaaa atatgtgcaa
     2401 tgccgggtaa acggcggaaa acgctgtgag gcgatgtggt gtcgcgaagc cccaggtgga
     2461 ccttccccgg acacaggcgt tcaaggacgc gcagacccat ctgcagggat tcaaaggatt
     2521 cggcaaaggt gaaatcgtaa tggggtgcaa gcggggccga gtcaaaacaa cttatatata
     2581 tggcttttgg tgtttctccg gggagggcag taatgccat
//
LOCUS       DAOSOF010000197         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_39295_length_2615_cov_4.947656, whole genome shotgun sequence.
ACCESSION   DAOSOF010000197 DAOSOF010000000
VERSION     DAOSOF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39295_length_2615_cov_4.947656"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLM68_08530"
     CDS             <1..1175
                     /locus_tag="PLM68_08530"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOT55438.1"
                     /translation="FVNDSSKGRIPVTLFLGGDPLYTLTGNFPHTADIDLMLLGGYLR
                     KKPIVKVPCLSQPLEVPENCDLVIEGYIEKNAQTVPTASCGENTGFYSIGGKEPLMHV
                     TCITHRKNPVIPLITPSLGLACSMRFVAKAFSIFVETNIEQSVALEARKLCYPDFSKQ
                     GAAAIVAVRKYYPGQVHKVANSFWGSDLTGLNKLLIVVDEHVNVFDRHQVNESIRKHY
                     NPSRDTFFSRGPLSLNDHAAPQRGFGGKICIDATDKHVQPQQVSSASDDPCLFYHKSE
                     TNPLSRNPEAKILIAVDEEVNLKKREMCYWLAINQADPIRDVNINNGILHVDACIKKQ
                     GDRGITRPWPNVCCSSLDTIQAVDNYWERLKIGKPVVSPSLQFHALLRPGNAQISE"
     gene            complement(1133..>2615)
                     /gene="lnt"
                     /locus_tag="PLM68_08535"
     CDS             complement(1133..>2615)
                     /gene="lnt"
                     /locus_tag="PLM68_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT55439.1"
                     /translation="LWMERAFTISGNRGAWKYYALTFLTWNVLTVFWISYSTLVGGIM
                     AILGNAFQMFSIFAVFRRVKRWILRKGKPEFIAHIFLAALWIAWEFFYFDAEISFPWL
                     VLGNGFATNVSLVQWYEMTGVLGGSLWIWVLNILVYYGCRVKPREWKSAAIPMVIMIV
                     LPITVSLIRFYTYKEPNLPVEVVVVQPNIDPYKEKFSSMPQSEQDKRILGLASSHITP
                     ETQLVVTPETSIREVVLGDYFSAPSMVLYKEFVQENPNTAFIFGATMVDFHPRAEKKP
                     TVASRPYSGGWYEIFNSAIQLDTTDRVQVYHKSKLVVGSEKMPYTNLFPFIENLALNL
                     GGTSGTLGTQSERTVFTSVNKGISVGVAICYESIYGGFITGYIRNGATLMAVITNDGW
                     WRNTPGYRQHVSYASLRAIETRRSIVHCANTGISALINQKGERIMQTKWWVQTAIRGT
                     VNLNEKLTPYVKYGDYTGWIVVFFMLLTGLYSLICALPGRKRA"
BASE COUNT          705 a          629 c          591 g          690 t
ORIGIN      
        1 tttttgtgaa tgactcctct aaaggcagga taccggtaac cttgtttctt gggggagatc
       61 cgctatatac gctcactggt aattttccac atacggcaga cattgatctt atgcttctgg
      121 gaggttattt acgtaaaaaa ccgatagtca aggttccctg cctgagccag ccgcttgaag
      181 tccctgagaa ttgtgatctg gtcatagagg gttatataga aaaaaatgca cagactgtac
      241 ctactgcgtc ctgtggcgag aataccgggt tttattccat tggcggaaaa gaacccctga
      301 tgcatgtaac ttgtatcaca cacaggaaga atcccgtgat tccactgatc acaccttcat
      361 tgggtttggc ctgttcgatg cgttttgtgg ccaaggcttt ctcgattttt gtagaaacaa
      421 atatcgagca gtccgttgcc ttggaagcgc gaaaactgtg ttacccggat ttcagcaaac
      481 agggtgctgc tgccatagtg gctgtgagaa aatattaccc cggccaggtt cataaggtgg
      541 ccaattcctt ctggggttcc gatcttaccg gcctgaacaa attactgatt gttgtggatg
      601 aacatgttaa tgtatttgac cgccaccagg tcaacgaatc tatcaggaag cattacaatc
      661 cgtcccggga cacatttttt agccgcggcc ccctgagtct caacgaccat gcagctcccc
      721 aaagagggtt cggaggaaaa atttgcatag atgcgactga taaacacgtt cagcctcaac
      781 aggtctcatc agcatccgat gatccctgtt tgttttacca taaatcagaa accaatccct
      841 taagcagaaa tcctgaagca aagatcctta ttgcagtgga tgaagaagtc aatctgaaaa
      901 aaagggagat gtgttattgg ctggctatca atcaggccga tcctatacgt gacgtcaaca
      961 taaataacgg gatcctccat gtagacgcct gcatcaaaaa acagggcgac cggggaataa
     1021 caagaccctg gccaaatgtg tgctgctctt cccttgatac aattcaggct gttgacaact
     1081 attgggaacg tttgaagatt ggaaaacccg ttgtttcccc atctcttcaa tttcatgccc
     1141 ttttgcggcc cggcaatgcg cagataagcg aataaagacc tgtcagcaac ataaaaaaga
     1201 caacaatcca gcccgtgtaa tctccatatt ttacataagg agtaagcttt tcattaagat
     1261 tgacagtacc tcttatggct gtctgaaccc accatttcgt ctgcatgatg cgttcacctt
     1321 tctggttgat caaggccgat atgcccgtat tggcacaatg gacaatgctg cgtctggtct
     1381 caatggcccg cagggatgca taactgacgt gttgcctgta tcccggggta tttctccacc
     1441 atccgtcatt ggttatgact gccatgagtg ttgcaccgtt cctgatatac ccggttataa
     1501 aaccaccata tatggattca tagcatatgg ccacacccac agatatcccc ttgttgactg
     1561 aagtgaatac ggtgcgttca ctttgagtcc ccagtgtccc ggaagttcct cccaggttca
     1621 gagccaggtt ttcaatgaag gggaacaggt ttgtataggg catcttctcc gacccgacaa
     1681 cgagttttga tttgtggtaa acctgtacac ggtcggtcgt atccagctga attgccgagt
     1741 tgaagatctc gtaccatccg ccggaatagg gacgggatgc cacagttggt tttttctctg
     1801 cccgaggatg gaaatccacc atagtggctc caaaaataaa ggcggtattg ggattttcct
     1861 gaacgaactc tttgtacagg accatggatg gtgcggaaaa ataatccccc aggactactt
     1921 ccctgatgct ggtttcagga gtgaccacca attgggtttc cggtgttatg tgcgaactgg
     1981 caagtcccag gattcttttg tcctgttcgc tttggggcat ggaagaaaac ttttccttat
     2041 agggatcaat gttgggctga accaccacta cctcaacggg cagattgggt tctttgtatg
     2101 tataaaacct tatcaaagag actgttatgg gaagcacaat cattatgacc atgggaatgg
     2161 ccgctgattt ccattccctt ggctttaccc tgcaaccata gtaaaccagg atattgagta
     2221 cccagatcca aaggctgccg cccaacactc cggtcatttc ataccattgc accaggctga
     2281 cattggtagc gaagccattg cccagaacga gccaggggaa ggaaatctcg gcatcaaagt
     2341 aaaaaaattc ccaggcgatc cataaagcgg ccaggaatat atgggcaata aattccggtt
     2401 ttccttttcg taaaatccat ctttttactc tcctgaaaac agcgaaaatg gaaaacatct
     2461 ggaacgcatt ccccaaaatg gccattattc ctcccaccag tgtactgtaa gatatccaga
     2521 atactgtcag cacattccag gtcaggaaag tcaatgcata gtatttccag gcccccctgt
     2581 tcccggatat agtgaaagca cgttccatcc ataaa
//
LOCUS       DAOSOF010000198         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_39596_length_2600_cov_4.826326, whole genome shotgun sequence.
ACCESSION   DAOSOF010000198 DAOSOF010000000
VERSION     DAOSOF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39596_length_2600_cov_4.826326"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLM68_08540"
     CDS             complement(<1..606)
                     /locus_tag="PLM68_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT55440.1"
                     /translation="MVKILIIEDDAAFNALLKTWLTKAGFRAEGVLSAGEAMRAVQRQ
                     VYDIVLSDLRLPDTDGLTLLKWIRETSPDSAVFIMTGYADIQTAVQAIRLGAVDYLEK
                     PVDPVILREKIDEVLRKKDRPHERIPFIKGESPASLKMYDYMMLVAPTLLSVLISGES
                     GTGKEYVANHIHQNSSRRDGPFVALDCGAIPRDLASSELFGH"
     gene            647..1180
                     /locus_tag="PLM68_08545"
     CDS             647..1180
                     /locus_tag="PLM68_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOT55441.1"
                     /translation="MMKARLFSVFLLVSLFYGSFSAVYGQSKTKVVNIPSVLEIEVII
                     PLSISKESNLSFGYASPGQGTGYVTLTATENPQRTASGVATLLPSPGAVYAGKFRVTG
                     GPNASVNVTVPTWIHTLTHSSGSPSLNLELTTPSATLQLSPAGEAVFYVGGTVTMWWG
                     QALGQYTGTFTVTVSYQ"
     gene            1325..1864
                     /locus_tag="PLM68_08550"
     CDS             1325..1864
                     /locus_tag="PLM68_08550"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOT55442.1"
                     /translation="MKTTLKIFALSVALFAFSNISFGQGNTAEATATAGANIITPIGI
                     TVDAGSKLQFGDLIATSSDYKVTIAPDGTRTSTGDAAFFTTTGFSAVSFTVTGEAGAA
                     FSITLPEDETIDLTGDGNDMALTDFTSSVGLSSSINGTAGSYNSGSKTFTVGATLSVG
                     ENQAAGQYSATFDVTVAYE"
     gene            1982..2509
                     /locus_tag="PLM68_08555"
     CDS             1982..2509
                     /locus_tag="PLM68_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOT55443.1"
                     /translation="MKKLILPLIAGLLFTGFSVNTYAIDPDPVVTATATADLVEALAA
                     SEKAQLNFGRFTAGASGGTVVIANTPAGTMNVTGTVTVIGGGSPSSAAFSITGQANQE
                     VTITLPADGAVNLTHATTTDVMNVATFSVSDASPKLSASGEATIYVGGTLTIPNTGIE
                     IGVYSGTYNVTFAYQ"
BASE COUNT          692 a          659 c          561 g          688 t
ORIGIN      
        1 gtgcccgaac agctcgctcg aggccagatc cctgggaatg gcaccgcagt ccagagccac
       61 aaaaggaccg tcccgccggg agctgttctg atgtatatgg tttgcgacat attccttgcc
      121 tgtgccactt tcaccggaaa tgagcacaga aaggagtgtt ggagccacca gcatcatgta
      181 gtcgtacatt ttcagggaag cgggactttc ccctttaata aaaggtatgc gttcgtgagg
      241 tctgtctttt ttccggagca cttcgtcaat cttttcccta aggatcaccg gatctaccgg
      301 tttttccagg taatcgaccg ctcccaggcg gattgcctgt acggcggtct gtatatcggc
      361 atacccggtc atgatgaaca cagcgctgtc gggcgaggtt tcccggatcc atttgagcag
      421 ggtaagtccg tcggtatcgg gcaaccttaa atcagaaagg acaatgtcat aaacttgtct
      481 ctgcacggcc ctcatggctt ctcctgccga tagaacgcct tctgcccgga atcctgcttt
      541 tgtcagccaa gtcttcaaca aggcgttgaa agctgcgtca tcctcaatga tcagtatttt
      601 aaccataaat gcaaatgtaa aaaatatttt tatgtatctt tgtcttatga tgaaggcccg
      661 tttattctct gtctttcttt tagtttccct tttctacggg agcttttctg ctgtttacgg
      721 acagtccaaa acaaaagtgg taaacatacc ttccgttctt gaaatagaag tgatcattcc
      781 cctgtctatc tccaaagaat ccaatctctc attcgggtat gcttctccgg gacagggaac
      841 aggatatgtg acgctcacag ccactgagaa cccgcaaagg acagcttccg gtgtggccac
      901 gcttttaccc agtcccggag ctgtttatgc cggtaaattc agggtaacgg gaggccctaa
      961 tgcgtcggtc aatgttactg tccccacatg gatccatacc ctgacccaca gcagcggctc
     1021 ccccagcctg aatcttgaat tgacaacccc ctctgccacc cttcagctga gccctgcggg
     1081 cgaggcagta ttttatgtgg gtggtaccgt caccatgtgg tggggccagg cccttggcca
     1141 gtacactggc acctttacag tgaccgtttc ataccagtga ttcccgggat tgttgaaaaa
     1201 aaacgccctt tcgtagattg ttttcagcct tatattgaaa tcgttttttt atttaaaacg
     1261 cagagcatac ttttgtcccg cgtttgaaaa attagtttaa aaaacaatct taaaaatatt
     1321 cattatgaaa acaactctca aaatcttcgc cctctcagta gccctcttcg cattctcaaa
     1381 catttctttc ggacaaggta acacagccga agcaactgca actgccggag ctaatattat
     1441 tacaccaatc gggataacag ttgatgcagg atccaaattg caatttggtg atttaattgc
     1501 cacttcatcc gattacaaag taaccattgc acccgatggc acaagaacct ccactggaga
     1561 tgccgccttt tttacaacaa ccggatttag tgctgtatct tttacagtaa caggagaagc
     1621 tggcgctgct tttagtatta ctttgcctga agacgaaact attgatctta caggagatgg
     1681 taatgatatg gctttaacag actttacaag cagtgtcggc ttatcttcaa gtataaacgg
     1741 aacagcgggc tcttataact ccggatcaaa aacttttacg gtcggtgcca cattatcggt
     1801 cggagaaaac caggcagctg gtcaatatag cgcaaccttt gatgtaaccg tagcttacga
     1861 ataataatag ctccacacga ctaatagctc cacacaataa tatatttcct cggtcgctcc
     1921 ttcccaatgg atggagcgtt ccggggaaaa ttaaaaaaaa gaaaaacgaa gtcaaacaac
     1981 catgaaaaag ctgatacttc ccctgatagc cggcctgtta ttcacaggtt tttccgtgaa
     2041 cacctacgca attgaccccg accccgtagt cactgctacc gcaacagccg atcttgtaga
     2101 ggcacttgcc gcctctgaaa aagcccagct caatttcggc cgttttactg caggcgccag
     2161 tggcggcact gttgtcattg caaacacccc tgcaggaacc atgaacgtta ccgggaccgt
     2221 gaccgttatt ggaggcggta gcccaagttc cgctgcattc agtattaccg gacaggctaa
     2281 ccaggaagtg accattaccc tgcccgcaga cggagctgta aaccttacac atgccaccac
     2341 tacagatgtg atgaacgtgg caacattttc agtctccgat gcaagcccca agctgagtgc
     2401 ttcaggtgaa gccactatat atgtgggcgg cacgctgacc attcccaaca ccggaattga
     2461 aataggtgtt tactccggaa cctataatgt aacctttgct taccaataag aaaagatgca
     2521 acccttaata tagggtataa cataagggga cttctataaa ttaacactat gatattctgg
     2581 atattatttg tatatttaat
//
LOCUS       DAOSOF010000199         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_39766_length_2591_cov_4.741719, whole genome shotgun sequence.
ACCESSION   DAOSOF010000199 DAOSOF010000000
VERSION     DAOSOF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39766_length_2591_cov_4.741719"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="PLM68_08560"
     CDS             complement(<1..1894)
                     /locus_tag="PLM68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOT55444.1"
                     /translation="MIVYQSTKEGFLEDVLNNEIDTKINQAFYDHLGRHTSPNEVLSW
                     TNSMMHMGNVLRDDEIASNAGISIEFQIPLTSQRIDFIITGLDEAQQEQVIIIELKQW
                     SSAQLTDKDAMVRTRFQHGETETVHPSYQAWSYAALIESYNQTVQEENIKLVPCAYLH
                     NYNPDDVITNPFYSYHIKKAPVFLKADAVKLREFIKKFVKYGDSKDILYRIENGRLRP
                     SKQLADSISAMIMGNREFIMIDEQKEVYETALSMSIKARSDKKMVLIVEGGPGTGKSV
                     VAVNLLAELTQRGQVTRYVSKNAAPREVYKVKLTGSLKKRMIDNLFGGSGDYCDIQEN
                     TFDTLIVDEAHRLNMKSGLYQNRGENQVKELIRAAKFTVFFIDNDQQIHIKDIGESNL
                     IARWAEKEGARVQRLKLKSQFRCNGSDGYLAWLENTLQLKETANIKLTPQEFDFRIVD
                     SPSELRKLILEKNSAKNQANILEKNSANKSRMVAGYCWDWASKKDPEAYDITFPQYDF
                     AMRWNLTKDGSTWIIAPDSVNEIGCIHTCQGLELDYVGVIIGPDIRFENGRVVTDVTK
                     RSSMDRSIFGIKTMLREDPQEALQLADRIIKNTYRTLLTRGMKGCYVYFCDQALHDHF
                     AAQLSPA"
     gene            complement(1932..2270)
                     /locus_tag="PLM68_08565"
     CDS             complement(1932..2270)
                     /locus_tag="PLM68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOT55445.1"
                     /translation="MNDYDRIIKKLIEFRNERDWEQFHDPRNLALALMLEAAELNELF
                     LWKKDHEVENIDIGRIKEELADVLTYSFFLAHKYNLDIFQIVEEKMIKNGQKYPREKA
                     RGTTKKYTEL"
BASE COUNT          579 a          610 c          647 g          755 t
ORIGIN      
        1 gggcagggga gagttgtgct gcaaagtggt cgtgcagcgc ctggtcgcag aagtagacgt
       61 agcagccttt catgccccgg gtcagaaggg tgcggtaggt gtttttgatg attcggtctg
      121 ccagttgcag ggcttcctgc gggtcttctc tgagcatcgt ttttatccca aagatggagc
      181 ggtccatgga ggaacgtttt gttacatcag tcactacacg gccgttttca aaacgtatgt
      241 cggggccaat aatgacgccc acatagtcca gttcaaggcc ctggcaggtg tggatacacc
      301 cgatctcgtt gaccgaatca ggggcaatga tccaggtgct gccgtctttg gtcaggttcc
      361 agcgcatggc aaaatcgtat tgcgggaacg tgatatcgta cgcctccgga tccttcttgc
      421 tggcccagtc ccagcaatag ccggccacca tcctggattt gtttgccgag ttcttctcca
      481 ggatatttgc ctggttcttt gcggagttct tctccaggat gagtttcctg agctccgagg
      541 ggctgtccac aatgcggaaa tcaaattcct gcggagtaag ctttatattg gccgtttcct
      601 tgagctggag cgtgttttcc agccaggcaa ggtagccgtc cgatccgttg caccggaact
      661 gggatttgag tttgagccgt tgcactcttg cgccttcttt ttctgcccag cgggcaatca
      721 ggttggattc gccaatgtct ttaatgtgga tctgctggtc gttgtcaata aagaagacgg
      781 tgaatttggc ggcacgaatc agttctttta cctggttttc gccccggttc tggtagagtc
      841 cgcttttcat gttcaagcgg tgcgcttcat ccacaatgag ggtgtcgaaa gtgttctcct
      901 ggatgtcgca gtaatcaccg ctgccgccaa agaggttgtc aatcatgcgc tttttcaggg
      961 agccggtgag tttcaccttg taaacttccc gtggtgctgc gttcttggaa acgtaccgtg
     1021 tgacctggcc gcgttgggtg agttctgcca gcaggttcac ggctaccacg ctttttcccg
     1081 ttcccggacc accttccaca atcagaacca tctttttatc gctacgagct ttgatggaca
     1141 tggaaagtgc cgtttcatag acctctttct gctcgtctat catgatgaat tcccggttgc
     1201 ccatgatcat ggccgatata ctgtctgcca gctgcttgga aggcctcaga cggccgtttt
     1261 ctatccggta caggatgtct ttgctgtccc catatttgac gaatttttta atgaattccc
     1321 ggagttttac agcatcggct ttcaggaaaa ccggagcttt ctttatatga tagctgtaaa
     1381 acgggttggt gattacgtcg tccggattgt agttgtgtag gtaggcgcag ggaaccagct
     1441 ttatattttc ttcctggacg gtctggttgt aactttcaat caatgcagca taggaccaag
     1501 cctggtatga agggtgtact gtctcggttt ccccgtgctg gaagcgggtg cggaccatgg
     1561 catccttgtc agtcagctgg gccgaggacc attgcttgag ctcaatgatg atgacctgtt
     1621 cctgttgtgc ttcgtccagc ccggttatga taaagtctat gcgctgggaa gtgagcggga
     1681 tctggaactc aatggatatg ccggcgttag aggctatttc atcgtcccta agcacgttgc
     1741 ccatgtgcat catggaattt gtccacgaca ggacctcgtt gggcgaggtg tgcctgccca
     1801 ggtggtcata aaaagcctga ttgatcttgg tatcaatttc gttgttcaga acatcttcca
     1861 gaaaaccttc ctttgtggac tggtaaacaa tcatagcata gtatttctca aagcatttct
     1921 cacagttatt ctcacagttc ggtatatttt tttgtggttc cacgagcttt ttcccggggg
     1981 tatttttgtc cgtttttgat catcttctcc tccacaatct ggaagatgtc caggttgtat
     2041 ttgtgggcca ggaaaaagga gtaggtaagc acgtctgcca gttcttcctt gatgcgcccg
     2101 atgtctatgt tttcaacttc gtggtctttc ttccacaaaa acaattcatt gagttcggca
     2161 gcctccagca tcagtgccag cgccaggttc ctgggatcgt gaaactgctc ccagtcccgt
     2221 tcattccgga attcaataag ttttttgata atgcggtcgt aatcgttcat aagcttgaca
     2281 ataaatcttt tacatccaaa gagtagtaaa gataatcaca taaattagaa cataaaagca
     2341 aaaagcagga gaatttgcgc agggtaaacc cggtttgccg cgcaacacga aaattgcaaa
     2401 aaataaatgc cagattttca ggcatttgaa aatctcaaaa tctctcttgc gcggcactcc
     2461 gggtttgccc ccccatcaat gaaacaaata tgtgataact ttacccaaaa tattatcata
     2521 tatggaagca gctctaccga taaaaatttg aaaaccttaa cagttaccat attatgaaac
     2581 ggtatagtat t
//
LOCUS       DAOSOF010000200         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_43820_length_2410_cov_4.883227, whole genome shotgun sequence.
ACCESSION   DAOSOF010000200 DAOSOF010000000
VERSION     DAOSOF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43820_length_2410_cov_4.883227"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLM68_08570"
     CDS             complement(<1..1007)
                     /locus_tag="PLM68_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOT55446.1"
                     /translation="MKKLFLILAMALISCTPRQDYNKKVWAWMVGKTTMTNGQWDYYF
                     QKAAEAGIDAILLECHGGYPEVLEEPSDTSKASTAASATGTDPAPDFTDHAAIQIIQN
                     ALPFAKKYGIELHAWIWAMNRTELSLRNAHPDWYQVNALGESCLNIKLYNREHYRWLC
                     PSRPETIQYLKERVAELAQIKGLAGVHLDFIRYPDAILPYGLQESRNVVQDRVYPQWD
                     FCYCDTCRACFKALTGVDPLDLEDPTADERWMQYRWDALSAVTSEVCAEIKKHGKVPS
                     VAVFATPQESRKLVRQDWPRFRNVDIFFPMIYHKFFNWEDYQVHTATREGVQELSEAG
                     N"
     gene            complement(1024..>2410)
                     /locus_tag="PLM68_08575"
     CDS             complement(1024..>2410)
                     /locus_tag="PLM68_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HOT55447.1"
                     /translation="HINLDVTQVGLNASVNGITELIQHDLLEGNGTAIKVNLQDECTM
                     DPSIAPGAPYEHPMSACRNLFLTGSTGFLGAFLIRSLIEQNEGKDIRIYCHVRAQNEE
                     KGRERVISNMKHYRCWKDEYAQNIQAVTGSLEQPRLGMREDVYQELSQHVDAVYHNGA
                     MLNFLFPYQFLKASNVDGTRECLRFACTGRAKYFHYISSYSVYDNPSHFGKKVYESDP
                     LASGEGYLLGYSETKWVSEKLAQSARERGLKACIYRPGDITGDTVNGIWEMKDLISRL
                     IVGCIHMQKAPDIKTRLFTVPVDYVSEAIAHISRQEGACNLAFNILNPESFTIKSMVK
                     AIRRMGYRIRIVPYESWREELRGTNIRENPLRVLASLFPEVSGTAGSDYATVTSDNAG
                     AADVAGDLGAAGATGPAGENLLNRFGRLQPRYDMTNTNNFLEDTEMQKRYLGKHLLTV
                     YLKYFMEQKYI"
BASE COUNT          525 a          641 c          611 g          633 t
ORIGIN      
        1 tcgttgccgg cttcactgag ctcctgcact ccttcccggg ttgccgtatg gacctggtag
       61 tcttcccaat tgaagaattt atggtagatc atgggaaaga aaatgtctac gttgcgaaag
      121 cggggccagt cctgccgtac cagcttgcgg gactcctggg gagttgcaaa tacggccacc
      181 gaaggcactt tcccgtgttt tttgatctcg gcgcaaacct cgcttgtcac tgccgagagg
      241 gcgtcccaac ggtattgcat ccagcgttca tctgctgtag gatcctccag gtcaagcgga
      301 tccaccccgg tcagggcttt gaagcaggcc cggcaggtat cgcaataaca aaagtcccac
      361 tgcggataca cccggtcctg tactacgttg cgggattctt gcaatccgta aggcaggatg
      421 gcgtcgggat aacgtatgaa atccagatgg acgcccgcca ggcctttgat ctgcgccagc
      481 tcggcaacac gttccttcag gtattgtatg gtttcgggac gggacgggca aagccagcgg
      541 taatgctccc ggttgtatag ttttatattc agacatgact ctcccagtgc attcacctgg
      601 taccagtcag gatgggcgtt gcgcaggctt agctcggtcc ggttcatagc ccagatccat
      661 gcgtgtagtt ctatcccgta tttcttcgca aacgggagtg cattctgaat gatctggatg
      721 gcagcatggt ctgtgaaatc cggggccgga tcagtacctg tcgcactggc agcagtggat
      781 gctttggacg tgtctgacgg ttcttccagc acttcggggt agcctccgtg acattccagc
      841 aggatggcgt caatccctgc ttctgccgct ttttggaaat aatagtccca ttgcccgttg
      901 gtcatggtcg ttttgccaac catccaggcc catactttct tattgtagtc ttggcgaggt
      961 gtacaagata tgagtgccat agccagtatc aggaataatt ttttcatgtt taaaagtaag
     1021 aaatcaaatg tatttctgct ccatgaagta cttaaggtag acagtaagca ggtgttttcc
     1081 cagatagcgt ttttgcattt ccgtatcttc caggaaattg ttcgtattcg tcatatcata
     1141 acggggttgc agccgcccga agcggttcag gaggttttca ccggctggac ctgtagcacc
     1201 agcagcacct aaatcacctg caacatcggc agcaccagca ttatcagagg taactgtagc
     1261 atagtcagag cctgcagtac cggaaacttc cgggaacaac gaagccagca cccgcagggg
     1321 attttccctg atgttggtcc cgcgcagttc ctctctccag ctttcgtaag gaacgatgcg
     1381 gatcctgtat cccatgcgcc ggatagcctt aaccatgctt tttattgtga atgattccgg
     1441 gttcaggata ttgaaggcca ggttgcaggc accttcctgc cgggagatat gggctatggc
     1501 ttcacttaca taatcaaccg gtactgtaaa gagccgggtc ttgatgtccg gggccttttg
     1561 catgtgaatg caccccacga tcagccggct gatcagatcc ttcatttccc atatgccgtt
     1621 gacggtatcc ccggttatat ctccgggcct gtaaatgcag gctttcaggc cgcgctccct
     1681 ggcactttgg gccagcttct ccgacaccca cttggtttct gaataaccga gcaaataacc
     1741 ctctcctgaa gccaggggat cggattcata tactttcttc ccgaagtggg aaggattgtc
     1801 atacacgcta taggacgaaa tgtaatggaa atatttagcc ctccctgtac aggcaaagcg
     1861 caggcattca cgcgtcccat ccacgttgct tgctttcagg aactggtatg ggaacaggaa
     1921 attgagcatg gctccgttat ggtaaaccgc atccacatgt tggctcaact cttggtaaac
     1981 gtcctcccgc atgcccagcc gcggctgctc cagggatcct gtaaccgcct gaatgttttg
     2041 tgcatattcg tctttccagc aacggtagtg tttcatgttg cttatgacac gttcccgtcc
     2101 cttttcttca ttctgtgccc gtacgtgaca atagatgcgg atgtctttcc cttcattctg
     2161 ttcaatgagc gaccggatca aaaaagcccc caggaagccg gtactcccgg tcaggaacaa
     2221 attcctgcat gcactcatcg ggtgttcata cggagcaccg ggagcaatgg acgggtccat
     2281 cgtgcattcg tcctgcaggt tgacttttat tgctgtcccg ttcccttcca gcaagtcatg
     2341 ctgtatgagt tccgtaatcc cgttcacaga agcattcagg cctacctggg tgacatccag
     2401 attaatatga
//
LOCUS       DAOSOF010000201         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_43851_length_2409_cov_6.002124, whole genome shotgun sequence.
ACCESSION   DAOSOF010000201 DAOSOF010000000
VERSION     DAOSOF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43851_length_2409_cov_6.002124"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLM68_08580"
     CDS             <1..788
                     /locus_tag="PLM68_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT55448.1"
                     /translation="VQGSCYGSTQPHATDFPLITIRDMVKAHELLRLHLGIEHIDLLT
                     GGSSGGFQALEWAIGNPGLTGNLCLIACNARISPWATALNESQRMALLADTTFGPREE
                     PLSGRRGLAAARSIAMLSYRSYEGYSKTQSEQEDDVLMALKACSYQQYQGKKLADRFS
                     PYSYYTLTLSLDSHNVGRNRGGTAKALSRIKARTLCIGIDSDILFPISEMEILARHIP
                     GAVLEVISSDFGHDGFLLEHEQITSTLCKFLNSGAAHWPMPLP"
     gene            737..>2409
                     /locus_tag="PLM68_08585"
     CDS             737..>2409
                     /locus_tag="PLM68_08585"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT55449.1"
                     /translation="MQVLKFGGSSLANASAISQVMKIIRTASARDKTIVVASALSGVT
                     DTLIRAGIMAGSGDSGYTTLIDDLQARHITVINELLPSESDPGVRVELSILFDKLRDI
                     CRGAFMIGEISPATSDLIAGFGELFSTRILEATCTSTGLSCHWADAREIIRTGPSSSQ
                     PVVNTAVTQRNVRELLRNNLSKIILVPGFIASDDQGRTTTLGRGGSDYTASLLAVAAK
                     ARMLWIWTDVNGMMTADPRIVPQARTIENISYKEALEISHFGARVVYPPTIQPVVSKG
                     IPIMVKNTFDPEGPGTLIEQDPPEGKEKIRGISGSDRIALLSMEGSGMVGIPGYSSRL
                     FNALAQQQINIILITQASSVHTMLVAIDQADAQKARKAADETFAYEISLQKVEPLKVE
                     SGFSIISLIGDDMKNQSGASGRMFEAIGRRNITIRAIAQGSSEKNVSAVVATNDAAEA
                     IRAVHQEFFGSSERCINLFIAGWGNVGKSLTAMIFSQREAWAVREGIRVQICGICNSK
                     RMILNKEGLAPDQIGQTLSSDGELYQNGTFIDKIIEMNLRNSVFADCTA"
BASE COUNT          607 a          628 c          639 g          535 t
ORIGIN      
        1 atgtccaggg gtcctgttac ggctccacgc aaccccatgc tactgacttc cctttaatca
       61 ccatccggga tatggtgaaa gcacacgaat tgcttcgcct ccatctgggt attgaacata
      121 ttgacctgct cacagggggt tccagcggag gttttcaagc cctggaatgg gccataggta
      181 atcccggcct gacaggcaac ctgtgcctga tcgcctgcaa tgcacgcatc agtccctggg
      241 ctacggcatt aaatgaatca caacgcatgg cccttttagc agatacgact ttcggccctc
      301 gggaagaacc cctgtctggc cgccggggac tggcagcggc acgatccatc gccatgctct
      361 cttaccggtc atatgagggg tatagtaaga cccagtcaga acaggaggat gacgtgctga
      421 tggctttaaa agcctgttct taccagcagt accaggggaa aaaactggca gaccggttca
      481 gcccttacag ttactacacc cttaccttaa gcctggattc gcacaatgtg gggcgtaatc
      541 gcggcggcac agccaaagcc ttgtcacgta tcaaagcacg caccctatgt attggcatag
      601 acagcgacat actttttccc atttcagaaa tggaaattct ggcccgtcac atacccggag
      661 cagtcctgga ggtcatttca tctgattttg gacatgacgg ctttttactg gaacatgaac
      721 aaataacatc tactttatgc aagttcttaa attcgggggc agctcactgg ccaatgcctc
      781 tgccataagt caggttatga aaattatccg gactgcctcg gccagggata aaaccattgt
      841 ggttgcctcg gccctgagcg gtgtgaccga tacgctgata cgtgcaggaa tcatggccgg
      901 cagcggagat tccggttata ctacattgat agacgatctg caagcaaggc atatcacagt
      961 tataaacgag ctgcttccat cggaatcaga tcccggtgtc cgggttgagt tatccatatt
     1021 gtttgataag ctgcgggata tatgccgcgg ggctttcatg ataggggaaa tcagtcctgc
     1081 cacttccgat ctgattgccg gtttcgggga gttgttctcc acccggatcc tggaagcaac
     1141 atgcacctcc acgggactaa gttgccactg ggcagatgcc cgtgaaatca tccggaccgg
     1201 tccctcctct tcccagccgg tggtcaacac cgccgtgacg cagcgcaatg tccgggaatt
     1261 gttgcgcaat aacctgtcaa agattattct tgtaccgggt ttcattgcct ccgatgatca
     1321 gggcaggaca accaccctgg gaaggggagg atcagattac acggcctcgt tgctggctgt
     1381 agcggcaaaa gcccgcatgc tttggatctg gaccgatgtg aacgggatga tgacggcaga
     1441 tccacgaatc gttccccagg cacgtaccat tgagaacata tcgtataaag aagcgctgga
     1501 gatctcgcat ttcggggcgc gggtggtgta ccctcccacc atacagccgg tcgtgtcgaa
     1561 aggcatcccc atcatggtaa aaaacacctt tgatcctgaa ggccccggga cactcatcga
     1621 gcaggacccc cccgaaggaa aagaaaagat caggggtatt tccggaagtg accgcatagc
     1681 cctgctatcc atggaaggga gcgggatggt gggcattccg gggtattcaa gccgactttt
     1741 caatgcgctg gcgcagcagc agatcaacat tattctcata acgcaggcat cttcggtgca
     1801 caccatgctg gtggctattg accaggccga tgcgcagaaa gccaggaagg ccgccgatga
     1861 aacctttgcc tacgaaattt cactgcagaa agtagaacct ttgaaagtag aaagcggttt
     1921 ttcaattatc agtcttattg gcgacgatat gaaaaaccaa tccggggcta gcggccgcat
     1981 gtttgaggcc attggccgcc gaaacatcac catccgggcc atagcccagg gatcctctga
     2041 aaaaaacgtt tcggctgttg tggccacaaa cgatgcagcg gaagccatcc gggctgtaca
     2101 ccaggagttc ttcggatctt ctgaacgctg tatcaacctc tttattgcag gctggggcaa
     2161 tgtgggaaaa agcctgaccg caatgatctt cagccaacgg gaagcatggg ccgtgcggga
     2221 aggtatccgg gtacagatct gcgggatctg caacagcaaa agaatgatcc tgaataagga
     2281 agggctggct cctgaccaga tcggacagac cttgtccagc gacggggaat tgtaccaaaa
     2341 cgggactttt attgacaaaa tcatagaaat gaatctgaga aacagcgtgt ttgcagactg
     2401 tacagctga
//
LOCUS       DAOSOF010000202         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_44297_length_2392_cov_6.429183, whole genome shotgun sequence.
ACCESSION   DAOSOF010000202 DAOSOF010000000
VERSION     DAOSOF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44297_length_2392_cov_6.429183"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..808)
                     /locus_tag="PLM68_08590"
     CDS             complement(248..808)
                     /locus_tag="PLM68_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT55450.1"
                     /translation="MKSREYYLDLLHRHITNPKMIAHSLASEAVLRALACRFGHDEDV
                     WGMAGLLHDIDVEITGSHPLRHGPEGALMLQDEDLPADVLDAIGMHNEKATGSRKRST
                     LFHHALAAGETVTGLIFAVALVYPDKKISSVKTKSVTKRMKEKIFAASVNRNTILECE
                     QMGISLDEFVSLALAALAPIEKELGF"
     gene            947..1582
                     /locus_tag="PLM68_08595"
     CDS             947..1582
                     /locus_tag="PLM68_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55451.1"
                     /translation="MKNLIIASVLFAFPLCLAAQTNFDHIKISGRQGEYITVLNVSST
                     TIEYENSFGVKKNIPVNEVEFLEYDGKVVYYNYRKNDHDIYDEYLQRISRRDPEKLLE
                     KGAKVFVPLIYGESRWEIMGCINTRKMLEEDHSDFWKLVDTEYEAEIILYYVVKYDDD
                     EIYHYYVIETRDGETIYKSDDEEIDGGWRASWDISREHVEDLLDNMIKDVD"
     gene            complement(1595..2134)
                     /locus_tag="PLM68_08600"
     CDS             complement(1595..2134)
                     /locus_tag="PLM68_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT55452.1"
                     /translation="MEFSELIKLRQSVRQYTDRSVEREKITACLEAARLAPSANNSQP
                     WKFIVVDDTELKNRVAECTASLGMNKFTYQAPVMVVVVLEKENLFSSLGGKVKDKIFC
                     HYDVGLAVGQFCLQAAELGLGTCIIGWFDEKKIRGILNINRKRRIPLIISLGYSEAPV
                     KEKKRKPMGEMSSWNAYNN"
     gene            2200..>2392
                     /locus_tag="PLM68_08605"
     CDS             2200..>2392
                     /locus_tag="PLM68_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55453.1"
                     /translation="MKHILLTALAILLASAAFAQVADSTGKEQIRYNQYGVPVNRRPL
                     FSEERNGVLVFESRNEDYKI"
BASE COUNT          621 a          568 c          549 g          654 t
ORIGIN      
        1 catgctggcc ctgtcccctg ccctggactg tctttcttcc cggctgagcg ggctggcgcg
       61 gaatccggtt tgcggaacag ttcttgtgac ggtcatctcg ttgcttttcg gctggcttaa
      121 ctggggcctc gccctggttt tcggagccat tctggtcaag aagatcgccg agcaagccat
      181 ccgtaccggc tctgccatta attaccccct gctgggagcc tggtctgttc cgctatcctg
      241 cacaggatta aaagccgagt tccttttcta tgggagccag ggccgccagg gccagggaga
      301 caaactcatc cagggaaatg cccatctgct cgcattccag gattgtattg cggttaaccg
      361 atgccgcgaa gatcttttct ttcatgcgtt ttgtaaccga ctttgtcttg acactggata
      421 tctttttgtc cggatagacc agtgccacgg caaagatgag ccctgttacc gtctctccgg
      481 ctgccagggc gtgatggaac aaagtagagc gctttctgct tccggtggct ttttcattat
      541 gcatgccaat ggcatcaagc acatctgccg gcaggtcctc atcctgcagc ataagtgctc
      601 cttccgggcc gtgcctcaac gggtgggacc cggtaatttc cacatcaata tcgtgtaaca
      661 acccggccat gccccagacg tcttcatcgt gcccgaacct gcacgccagg gccctgagaa
      721 cggcttcgga agcaaggcta tgggcaatca ttttgggatt ggtaatgtgg cggtggagca
      781 gatccaggta gtattcgcgg gatttcatat tgtccggtcg tattattcgg ttggaaaata
      841 ataaagcaat aaaatgacgt gataaagata caaaagtatt gacaggattc acgtatcatt
      901 ttttttcata tatttactaa ttgttaatca actcaatgaa agcattatga agaatctgat
      961 cattgcatct gttttatttg cctttcccct gtgccttgcc gcacaaacaa attttgacca
     1021 tatcaaaata tcgggaaggc agggagagta cataactgtt ttaaatgttt catccaccac
     1081 catcgaatac gaaaattcgt ttggtgtgaa aaaaaatatc cctgtaaacg aggtagaatt
     1141 tttggagtac gacggcaaag tggtttatta taattacagg aaaaatgacc acgacatcta
     1201 tgatgaatac ctgcaacgga tatcacgccg ggacccggaa aaattgctgg aaaaaggtgc
     1261 caaggttttt gtaccgctca tttacgggga aagccgctgg gagatcatgg gttgcatcaa
     1321 cacccgcaaa atgcttgaag aagatcacag tgatttctgg aaactggtcg ataccgaata
     1381 cgaagccgaa atcatactct attacgtggt aaaatacgac gacgatgaaa tttatcacta
     1441 ttatgtaatt gaaacacgtg atggtgaaac aatctacaaa tcagatgacg aagaaattga
     1501 cggaggctgg agggcctcct gggatataag ccgggagcac gtagaggatc tgttggacaa
     1561 catgatcaag gatgtcgact aaaaaaggtg tcttttagtt gttgtacgca ttccagctgc
     1621 tcatttcacc cattggcttt cgtttctttt ctttcactgg tgcttctgaa tagccgagtg
     1681 aaataatcag cggaatacgc cttttcctgt tgatgttcaa tattcccctg atcttttttt
     1741 catcaaacca cccgatgata caggttccca ggccgagttc agccgcctgc aggcaaaact
     1801 ggccgactgc caggcccaca tcataatgac agaaaatctt gtcttttacc ttcccgccca
     1861 aagaggaaaa cagattctct ttctccagta ccactaccac catgacaggc gcctggtacg
     1921 tgaatttgtt catacccaga ctggcagtgc actccgctac ccggttcttc agttccgtat
     1981 catctaccac aatgaatttc cagggctggg agttattggc cgagggagca agccgggccg
     2041 cctcaaggca tgcagtgatt ttttcccttt caacgctccg atccgtgtat tgacggacac
     2101 tttggcgtaa tttgattaat tccgaaaatt ccatatacaa atatactaac ataatttttt
     2161 attattttta tagagatttt aacctaatta attaatagta tgaaacatat tctattgaca
     2221 gcactggcta tcctgcttgc ttccgcagct tttgcccagg tagctgattc tacgggaaaa
     2281 gaacagatcc gctacaatca gtacggggtg ccagttaacc gcagaccgtt gttttccgaa
     2341 gagcgcaacg gtgtcctggt atttgaatcc aggaatgaag actacaagat ct
//
LOCUS       DAOSOF010000203         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_44778_length_2375_cov_3.237931, whole genome shotgun sequence.
ACCESSION   DAOSOF010000203 DAOSOF010000000
VERSION     DAOSOF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44778_length_2375_cov_3.237931"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="PLM68_08610"
     CDS             complement(<1..1556)
                     /locus_tag="PLM68_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT55454.1"
                     /translation="MRKSPVYCSLLLTVSLFAFSLKAPAQDAVVKKIIHEGTTNNQTQ
                     KHLDILTNRIGGRPVGSDAYNAAVQWAAAQFESWGLEVSIEEAGETRVGFSRGPWSGK
                     IYGGILPGGDGIHLHFATPSYTSGTIGLQRGHVVIEPKTQAEFDRMKGVLKGAWVLVT
                     GTNTGWPVDRSVKGDQRRDSLIRLNEEVARHNNDVMRRYRARSGDRRMNIDEMQEALK
                     DSLLKTSDEPALFYRQMADAGILGLIQSAPVPITALWDKETAFGETSSFENLPSIPDI
                     KLDEHQYAYIYQLAKERQPIALEFDIRNHFRPGPVKFHNVVAKITGTHYPDQYVMVSG
                     HLDSYDVAAGGVDCGVGVTPAMESARLLSVAGAKPKRSILFVLFAGEEFGLLGATAWV
                     EQHKDELERISNLFNRDGGPLAATGVAVPKAMYEDFVKASKHISDINPDFPFTVDTIP
                     PRPMPAQPGGTDASVFAIKGVPTISLRDRDVKGYDFNYREIWHTERDLYTKSIGEYQE
                     HTSVVQAVIV"
     gene            1616..2191
                     /locus_tag="PLM68_08615"
     CDS             1616..2191
                     /locus_tag="PLM68_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOT55455.1"
                     /translation="MIRFREIVSAFMVLFAVIDITGSIPIILDMKAKGRKFKPFLATI
                     ISTALIFLFLFVGEAILGLFSVDISSFAVAGSLVIFVLAVEMIFGVEIFKNDGPPDAS
                     TIVPLVFPLIAGTGTLTTVLSLRAEYSLPNVGIAIVLNMIIVYVVLRHVNLAEKLLGK
                     GGIYILRKFFGIILLAISVKLFASNVTGLFT"
BASE COUNT          516 a          602 c          606 g          651 t
ORIGIN      
        1 tatacgataa ctgcctgtac aacagatgtg tgctcctgat attctcctat gctcttggta
       61 tacaagtcac gctccgtatg ccagatttcc ctgtaattga agtcgtatcc cttcacatcg
      121 cggtcacgca atgagatggt gggtactccc ttgatggcaa agaccgaggc atctgttcct
      181 ccgggttgtg ctggcatggg acgcggggga atggtgtcca cggtaaacgg gaaatcagga
      241 ttgatgtccg aaatgtgttt cgaggctttt acaaaatctt cgtacatggc tttgggtacc
      301 gctaccccgg tggcagccag tggtcctccg tccctgttga agaggtttga gatccgctcc
      361 agttcgtctt tatgctgttc aacccacgcg gtggctccga gcaacccaaa ttcttcgccg
      421 gcaaatagca caaacaggat gcttctttta ggttttgccc cggcaacgga aagcagcctg
      481 gccgattcca tggcaggggt aacccctacg ccgcaatcca cacccccggc agccacatca
      541 taggaatcca ggtgaccact caccatcacg tattgatccg ggtaatgcgt tccggtgatt
      601 ttggcgacca cgttatggaa cttgaccggg ccgggccgga aatggtttct gatgtcaaac
      661 tccagtgcta tgggctgtct ttcctttgcc agttggtata tatatgcgta ctggtgttca
      721 tccagtttga tatcgggtat tgagggcagg ttctcaaaag aggaagtttc gccaaaagct
      781 gtttctttat cccacaatgc ggtgatgggg acaggagcag actggatcag tcccaaaatg
      841 ccggcgtctg ccatctgcct gtaaaacagg gctggttcgt ctgaggtttt aagtaaactg
      901 tctttcagcg cctcctgcat ttcgtcaata ttcattctcc tgtcacccga gcgggcacgg
      961 tagcgccgca tgacatcgtt attgtgacgg gccacctctt cgtttagccg gataagcgag
     1021 tccctgcgtt ggtcgccctt tacggacctg tccacaggcc agccggtatt ggttcctgtt
     1081 accaacaccc aggctccttt gagaacgccc ttcatccgat caaattctgc ctgggttttg
     1141 ggctctatga ccacatggcc acgttgcaat cctatagtcc ccgacgtgta agagggagtg
     1201 gcaaagtgca ggtgaatgcc gtcacccccg ggaagtatcc cgccataaat ttttcctgac
     1261 caggggccgc ggctgaatcc cacgcgtgtt tcgcctgcct cttcaatgga cacctcgagt
     1321 ccccaggatt caaactgtgc agcagcccac tgtactgccg cattgtaggc gtccgacccc
     1381 acaggccgtc cgcctatccg gtttgtaagg atgtccaggt gtttctgcgt ctggttattg
     1441 gtggttccct catggatgat ctttttgacc acggcatcct gtgccggtgc tttgagggaa
     1501 aatgcaaaga gacttactgt caacagcaag ctgcaataaa cggggctctt tctcatctga
     1561 tataatgtgt ttgaatacaa gtataacaaa tgtagtgttt ttttatcttt gtagtatgat
     1621 caggttcagg gaaatcgtca gtgcgtttat ggttttattt gccgtgattg acattactgg
     1681 ttccataccc attattcttg atatgaaagc caaaggaagg aaattcaagc ctttcctggc
     1741 gaccatcata tccactgccc tgatattcct gtttttattt gtgggagagg ccatccttgg
     1801 actgttcagt gtagacattt cttcttttgc cgttgccggg tcactggtga tctttgtgct
     1861 ggcagtggaa atgatatttg gcgttgagat cttcaaaaat gacggacctc ccgatgcatc
     1921 cacgatcgtt cccctggttt ttccgctgat agccggaaca ggaaccctta ccaccgtgct
     1981 gtccctgcgg gccgaataca gcctgcccaa tgtaggcatt gccattgtgc tcaacatgat
     2041 cattgtgtac gttgtattga ggcatgtgaa tctggcagaa aaactgctag gcaaaggagg
     2101 aatctacatt ttgagaaaat tcttcggcat catcctgctg gccatatctg ttaagctgtt
     2161 cgcttctaac gtaacagggc tgtttacctg attggctatg aaaaaaaagg ttttgctctt
     2221 aatcatgtct gctttttccc tggcttgcgg atgctgtaag gatgaacccg ccccgggaac
     2281 tgatccggtg gagcagccgg actggatagt gggggcggac ctctcgctgc tttcaagaat
     2341 ccagcaggca ggaggcgtat acaaggacag ccagg
//
LOCUS       DAOSOF010000204         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_45747_length_2336_cov_4.249014, whole genome shotgun sequence.
ACCESSION   DAOSOF010000204 DAOSOF010000000
VERSION     DAOSOF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45747_length_2336_cov_4.249014"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLM68_08620"
     CDS             complement(<1..726)
                     /locus_tag="PLM68_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT55456.1"
                     /translation="MKKTCLFILCSLCILQAAGQYRTPTDPKPSGLRVFTTRGESFTL
                     WLDGFADLDGSYFFGYYEDYDSIPNGGTVRNARLGLRAEVADGWTGVFEADFSRLKPR
                     ITEAYIQYSGIRNLNITAGHFESLFSMEGAARPREQAFHEKPMVISAMVPVDLLGIQV
                     STNQYWFLGSLGVSFDKTSYKRQNPEADSILTTSFDSRTVMAKVVAMPFVNNKDLGLH
                     VGVAASYKFPAKDPVTGSYPSIRY"
     gene            complement(773..1258)
                     /locus_tag="PLM68_08625"
     CDS             complement(773..1258)
                     /locus_tag="PLM68_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOT55457.1"
                     /translation="MKCKYILQVFLFLLAAQTVKAQPSDLQIDILNNFNFGKVAVTGW
                     SGSVSIEVANGVFNRVATGSVELKDMGNYSPATIKFSSSSKNFNVTQLILPGEVTLTR
                     QGGSQTRTIYSITAWPPPPYYSIKKGITVYMGGTIQLADYQANPGGIYSGNLSFTVVY
                     E"
     gene            complement(1278..2120)
                     /locus_tag="PLM68_08630"
     CDS             complement(1278..2120)
                     /locus_tag="PLM68_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55458.1"
                     /translation="MHKSGKLFFLLVFFIFTGSLMPRAFAQGNLLVTPRRVVFDGSRR
                     VMELNLANTGQDTARYNISFIQYRMTTEGTFEEITEPDPGQFFADKHLRFFPRSVTLA
                     PNEAQTVRMQVVGRENLAPGEYRSHVYFRAVPNEVALGEEDPNRDTTSVSVRLIPIFG
                     ITIPVIIRVGDSATEVYLSDLALEKGDNEALYLKMSFNRSGNMSVYGDLNVTHVAPNG
                     TETRVGVVNGIAVYTPNTIRNFRIALDNQSGVDFSSGKLLITYTAQSDVRPETYATAQ
                     LELP"
BASE COUNT          634 a          541 c          549 g          612 t
ORIGIN      
        1 gtagcgtatg gacgggtatg atccggtaac cgggtccttt gcgggaaatt tataggaagc
       61 cgccacgcct acatggagtc ccaggtcctt gttgttcaca aagggcatgg ctacaacctt
      121 tgccataacc gtacgcgagt caaaggatgt ggtcagtatg gaatccgctt cgggattctg
      181 ccgcttgtaa cttgtcttgt caaaagaaac cccaagagag cctaaaaacc agtactggtt
      241 ggtgctgacc tggataccca acagatccac cgggaccatg gccgatatta ccatgggttt
      301 ttcatggaaa gcctgttccc ggggccgggc agcgccttcc atagaaaaga ggctttcaaa
      361 atgtcctgct gttatattca gattacggat accggaatac tgaatataag cttcggtgat
      421 gcggggtttg agcctggaaa aatccgcctc gaaaacgcca gtccagccat ctgccacttc
      481 tgctctcaac cccaggcgcg cattgcgtac ggttcctccg ttcggtatgg aatcataatc
      541 ttcataatat ccgaagaaat aactgccgtc caggtcagca aatccatcca gccacaatgt
      601 gaatgattcc ccgcgggttg taaaaacacg gagcccggaa ggtttcgggt ctgttggggt
      661 gcggtattga cctgctgcct gcaggataca aagagaacac aagataaaca agcacgtttt
      721 cttcattctg gctattataa gagagcgtag atgacggagg ctggaggtac ggttattcgt
      781 aaacaacggt gaaactaagg ttccctgaat aaattccccc gggattggcc tggtaatcgg
      841 caagctgtat ggttccaccc atatacacgg taatcccttt ttttatgcta taatatgggg
      901 gaggaggcca tgctgtaata ctataaatcg tcctggtctg actgccgcct tgcctggtaa
      961 gggttacttc acccggaaga atgagctgtg ttacattgaa atttttactg ctggaggaaa
     1021 actttatcgt tgcaggacta taattaccca tatctttaag ctccaccgac cctgtcgcca
     1081 cacggttaaa aaccccgttg gctacctcta ttgatacaga acctgaccaa cctgtaactg
     1141 ctactttgcc aaaattgaaa ttattaagga tatcaatctg caagtcactt ggttgagcct
     1201 tgactgtctg tgctgctaac agaaacagaa aaacctgcaa gatgtattta catttcataa
     1261 aacctggtta aagcttttca gggcaactcc agttgtgctg tagcgtatgt ctccggcctg
     1321 acgtccgatt gggcagtata tgttatcagt aatttgcccg agctgaaatc caccccggac
     1381 tgattatcaa gtgctatgcg aaaattcctt atagtatttg gggtgtatac agctatacca
     1441 ttgactacgc ctacccgggt ctccgttcca ttgggtgcga catgggtgac attcaggtcc
     1501 ccgtatacag acatgtttcc tgatcggttg aaactcattt tgagataaag agcttcatta
     1561 tcaccctttt ccaaggccaa gtctgaaagg tacacctctg tggcggaatc tcccacgcgg
     1621 atgataaccg ggatggtgat tccaaaaatg ggtatcagcc ggacactaac cgaggtggtg
     1681 tcacggttgg gatcttcctc gcccaaggcc acttcattgg ggacagcgcg aaaatatacg
     1741 tgggaacggt attcgcccgg tgccagattt tcccttccca ccacctgcat acggaccgtc
     1801 tgggcttcat tgggtgccag ggtcactgaa cgcgggaaaa aacgaaggtg cttatcggca
     1861 aaaaattgac cgggatctgg ttctgtaatt tcttcaaaag ttccttctgt ggtcatcctg
     1921 tactggatga aagaaatatt ataacgggcc gtgtcttgtc ctgtattggc caggttcaat
     1981 tccattaccc tgcgggatcc gtcaaacacc acacgccgtg gggtgacaag caaattcccc
     2041 tgggcaaatg cccggggcat taaggagccg gtaaaaatga aaaataccag caaaaagaaa
     2101 agttttccag atttatgcat gatcccttaa tttatacgtt gacaataaac acaaatataa
     2161 gaaaaaatta aaacgtaaca gatttacggg aaagagatta ttaattcggg aaagatcagt
     2221 gatgattccg tcatttattt tatcaaaaaa cgcttctctg caagggagaa gcgttcctta
     2281 tggtgggttc taaaaattca ttttccccgt tttttgattt gaaaaatcga agaacc
//
LOCUS       DAOSOF010000205         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_46625_length_2304_cov_3.824811, whole genome shotgun sequence.
ACCESSION   DAOSOF010000205 DAOSOF010000000
VERSION     DAOSOF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46625_length_2304_cov_3.824811"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLM68_08635"
     CDS             <1..285
                     /locus_tag="PLM68_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT55459.1"
                     /translation="KAVKIPLVCLGGLFSRCIMEQALQDGFEFIGMARPLINDPAFVN
                     KMKKAEEEGNGDVRSGCKHANYCIGRMYSLDMVCHQHVKNIPKRLLDEFK"
     gene            272..1123
                     /locus_tag="PLM68_08640"
     CDS             272..1123
                     /locus_tag="PLM68_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT55460.1"
                     /translation="MNSNNTPVALVTGANGGMGREITRALLRSGYRVVVSCRPGKRGL
                     DFYNLLSQEFGSDRVDYLDMDLSCEISVRDAARRLLEKESRLDVLVNNAGMLGWKPEV
                     NKKGYEMHNMVNCLGPMLFTWLLKPILGRGSRIVNTVSVMIRVGRIPPHFPYPPEKFD
                     RFKQYACSKLALTLLSLRLAQLWKEDGITVNMTDPGIVNTPIIRLHKWIDPLTDVFFR
                     PVIRQAPRGAATAIFLSLDPSVEGKTATLFKDCRAVRLSSRVLDDPGGADLWSFFITL
                     LSYGDQQ"
     gene            1107..1871
                     /locus_tag="PLM68_08645"
     CDS             1107..1871
                     /locus_tag="PLM68_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55461.1"
                     /translation="MVISNKINMGFLVVGFVLFLSSLVSIFEMGRTRHSLGDVLSVNV
                     DNINASIQLLEVTDQNIFTLLNQIGVNPDSLLQMGSLYEDDRFEGYFRASRATFTTKE
                     EQALTDSIMFAYAAYMQILNEAPVIWEKNGYQARREWYTARLYPTYTRLRNYIQELIS
                     LEQRLLDHNTQQIQDGFYRSIMPGVIAVASSILLLFLLSYFIRIYIIQPIKEIRTGVR
                     NTLLFKKKYQVRLPAGDELSELNESITQLCDEHNKL"
     gene            1868..>2304
                     /locus_tag="PLM68_08650"
     CDS             1868..>2304
                     /locus_tag="PLM68_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT55462.1"
                     /translation="MRIQVIARYMGLVLLVNALFMFVSVIISAIYGFDTGFSPLLLSA
                     IITATIGSFPYIFVRESSEVTTREGYVIVVLSWILSCLFGMLPYLLWGGEFTLINAWY
                     ESVSGFTTTGGTILTNIEAIPHSLLFWRASTHWIGGMGVVLFM"
BASE COUNT          558 a          531 c          581 g          634 t
ORIGIN      
        1 aaagccgtaa aaattcccct ggtttgtctg ggaggccttt tttctcgttg catcatggag
       61 caggcacttc aggatggttt tgaatttatt ggcatggcac gtcccctgat caacgatcct
      121 gcctttgtga acaagatgaa aaaggcagag gaagaaggta acggggatgt cagaagcggc
      181 tgcaaacacg cgaactactg cattggcagg atgtacagcc tggacatggt atgccaccag
      241 catgtgaaaa atattccaaa acgtttgctt gatgaattca aataatactc cggttgccct
      301 ggtaaccgga gctaacggtg gaatgggaag ggaaatcacc cgtgcgctgc tccgctcagg
      361 ttaccgtgtg gtcgtatcgt gtcgcccggg caaaaggggt cttgattttt acaacttgct
      421 cagccaggaa tttggaagcg accgggttga ttacctggat atggaccttt cctgtgagat
      481 ttctgtacgg gatgcagcac gccggttgct ggaaaaggaa tcccgcctgg atgtgctggt
      541 taataatgcc ggaatgctgg gatggaagcc cgaagtgaac aagaagggtt acgagatgca
      601 caatatggtc aactgtctgg ggccaatgtt attcacctgg ctgctgaagc caatactggg
      661 caggggaagc cgcatagtga acaccgtttc tgtgatgata cgtgttggac ggatccctcc
      721 tcattttccg tatccgccgg aaaaattcga ccggtttaaa cagtatgcct gttccaaact
      781 ggcgcttacg ttactttccc tgcgtctggc gcaattatgg aaagaagacg gcattactgt
      841 aaacatgacc gatccgggaa ttgtcaatac ccccatcatc aggctccaca aatggattga
      901 cccccttacc gatgtgtttt tcagaccggt aatccgtcag gctcccagag gggccgcgac
      961 agccatcttc ctgtcccttg atccgtctgt agagggaaaa acggcaacct tgtttaagga
     1021 ttgccgggcg gtcaggttgt cttccagggt actggatgat cccggaggcg ctgacctatg
     1081 gagttttttt ataactttgt taagttatgg tgatcagcaa taaaatcaac atgggttttc
     1141 ttgttgtggg ctttgtcctg ttcctgtcaa gccttgtcag catttttgaa atggggagga
     1201 cccggcattc cctgggcgat gtgctttctg tcaatgttga taacatcaac gcatccatcc
     1261 agttactcga ggttacggac cagaatattt ttacattgct caaccagata ggtgtcaacc
     1321 ctgattcctt acttcaaatg ggatccttat acgaggatga ccggtttgaa ggctattttc
     1381 gtgcttcccg ggctacattt accacaaaag aggagcaggc acttactgat tccataatgt
     1441 ttgcctatgc tgcttatatg cagatcctga atgaagctcc tgttatttgg gagaaaaatg
     1501 gttaccaggc caggagggag tggtataccg cacggctgta tccgacttat accaggttga
     1561 gaaattacat tcaggaactc atatccctgg aacaacgtct gcttgatcac aatacgcaac
     1621 agatccagga tggattttac aggagtataa tgccaggggt tattgcagtg gccagcagca
     1681 tattgctgct tttcctgctg tcctatttta tcaggatcta tattatccag cccataaaag
     1741 agatccgtac aggggtcagg aatacattac tctttaaaaa gaagtaccag gtcaggctgc
     1801 ctgccggtga cgaactgtcg gaactgaacg agtccattac gcaactctgc gacgaacaca
     1861 ataaattatg agaatccagg tgatagcaag gtacatgggc ctggttctgc tggtaaatgc
     1921 cctgttcatg ttcgtttccg ttattatatc ggcaatttac ggttttgata ccggtttttc
     1981 ccccctgttg ctgtctgcta tcattacagc cactattggt tccttccctt acatatttgt
     2041 cagggagtcc agcgaggtaa ccacccggga aggctacgtg atcgtggttt tatcatggat
     2101 cctgtcctgt ctctttggta tgctgcctta cctgctctgg ggcggtgaat tcacactcat
     2161 caatgcctgg tatgaaagcg taagcggctt caccacaacc gggggaacta tacttacaaa
     2221 tattgaagcc atcccgcatt ccctgctttt ctggcgtgcc tctacccatt ggatcggggg
     2281 gatgggagtc gttcttttca tgtt
//
LOCUS       DAOSOF010000206         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_47418_length_2275_cov_5.200450, whole genome shotgun sequence.
ACCESSION   DAOSOF010000206 DAOSOF010000000
VERSION     DAOSOF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_2275_cov_5.200450"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="guaA"
                     /locus_tag="PLM68_08655"
     CDS             <1..984
                     /gene="guaA"
                     /locus_tag="PLM68_08655"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT55463.1"
                     /translation="FALGICQCKGDWTPSGFIEETIAGMREKIGDEHVIMAISGGVDS
                     TVTATLLHKAIGTQLHCIFVDTGLLRLNEYTAVMRTYQDMGLNITGVDASSTFLGALK
                     GITDPETKRKTIGRIFIEVFTREASKVRNATWLAQGTIYPDVIESVSLHGHTSAIKSH
                     HNVGGLPKHVPLKIIEPLRLLFKDEVRCIGLALGVKEELIKRHPFPGPGLAIRLLGEI
                     TLEKLQILRQADDIFIQSLKKENLYDTVWQAGAILLPVRSVGVMGDERTYEYTIALRA
                     VESTDGMTADWIHLPYDFLMKVSNDIINKVKGVNRVVYDISSKPPATIEWE"
     gene            complement(939..1547)
                     /locus_tag="PLM68_08660"
     CDS             complement(939..1547)
                     /locus_tag="PLM68_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOT55464.1"
                     /translation="MQPFLPRGSRILMLGSFPPPKTRWSMDFYYPNFRNDMWRIVGLV
                     FFNQAGYFIQGNGYNRKLIESFCREKGLALYDAAVKVIRMTGNASDKYLEVVESIDLA
                     GILDNLPMCSNVAVTGQKAAQTVLEVLAGTGFQQVLQLPAMGSFANFRYGERLVRLYR
                     MPSTSRAYPMPLEKKVSYYRNLFKSFLGHSHSMVAGGLEDIS"
     gene            complement(1564..1749)
                     /locus_tag="PLM68_08665"
     CDS             complement(1564..1749)
                     /locus_tag="PLM68_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55465.1"
                     /translation="MKCETSKSAPAGGAVYGIGFVGAVIYFIVQASGFWEGVLGVLKA
                     IVWPAIMVFEVLKYIGA"
     gene            1879..>2275
                     /locus_tag="PLM68_08670"
     CDS             1879..>2275
                     /locus_tag="PLM68_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55466.1"
                     /translation="MKVGLTYDLQSEYLSLGYSEEDTAELDKTETIEGIETALHSLGY
                     ETERIGNARSLMMRLFKGDRWDLVFNICEGIFGDGRESLVPAILDDWQIPYVFSSAAT
                     MALSLNKALCKRVVRDAGIPTPDFCLVRSI"
BASE COUNT          640 a          537 c          531 g          567 t
ORIGIN      
        1 ttcgcattag gtatctgcca atgcaaaggg gactggaccc cttccggctt tatagaggaa
       61 accatagccg gaatgcgcga aaagattggt gatgaacatg tgatcatggc catatccggg
      121 ggcgtggaca gtaccgtgac agcaaccttg ctgcacaaag ccataggaac acaattgcat
      181 tgcatatttg tggatacggg tttgttacgc ctgaacgagt atactgctgt catgagaacg
      241 taccaggata tggggttgaa tataaccggt gtggatgcat cctcgacttt cctcggggcc
      301 ctgaagggaa taacagatcc tgaaaccaaa cgcaagacga taggccggat ctttattgag
      361 gttttcacca gggaagcctc aaaggtccgg aatgccacat ggcttgccca ggggaccatc
      421 tatccggatg tcattgaatc tgtttcttta cacggtcata catctgccat taaatcacat
      481 cacaatgtgg gaggccttcc caagcatgtt cccttgaaaa ttatagaacc cctgcgcctg
      541 ctcttcaaag atgaagtacg ctgcatagga cttgcccttg gtgtgaagga agagttgatc
      601 aaacgccacc ccttccccgg tcccggtctg gctatcaggc ttttaggtga aatcaccctt
      661 gaaaaactac aaatcctcag acaagccgac gatattttca tccagtcgct aaagaaagaa
      721 aacctgtatg atacggtctg gcaggccgga gcaattctgt taccggtcag gagtgtgggt
      781 gtcatgggtg atgaacgcac gtatgaatac accattgcct tacgggcggt tgaatctaca
      841 gatggtatga cagctgactg gatacacctg ccttacgact tcctcatgaa agtttcaaac
      901 gatatcatca ataaagtaaa gggggtcaac cgggttgtct atgatatatc ttccaaaccc
      961 cctgcaacca tagaatggga gtgacccagg aagcttttga ataaattcct gtaatacgac
     1021 acctttttct ccaaaggcat gggataagcc cttgaagtag agggcatcct gtacaatcta
     1081 accagtcttt ccccatatcg gaaatttgca aaagatccca tggctgggag ctgcagtacc
     1141 tgttgaaaac cggttccagc cagcacttca agcaccgtct gggccgcttt ttgtcctgtt
     1201 acagcaacat tgctgcacat agggagattg tccagaatcc ctgccaggtc aatggattcc
     1261 acaacttcca ggtatttatc agacgcattc cctgtcatgc ggatcacctt taccgccgca
     1321 tcataaagag ccagaccttt ctccctgcag aaagactcga taagtttcct gttgtatcca
     1381 ttcccctgaa tgaaataacc ggcctggttg aagaagacca ggcctactat gcgccacatg
     1441 tcattcctga agttgggata ataaaaatcc atagaccacc gggtcttcgg aggaggaaaa
     1501 ctgccaagca taaggatgcg tgaccctctt ggcaggaatg gttgcatggg gtggttttcc
     1561 ggttcaggca cctatgtact ttaacacttc aaaaaccatg attgccggcc atacaatagc
     1621 tttgagaaca cccaggacac cttcccagaa gccactggcc tgaacaataa agtagattac
     1681 tgccccgaca aagccgatac cgtaaactgc ccctccggcg ggagcacttt ttgacgtttc
     1741 acatttcatg aaaattcttt ttaaaaaaat aatttgaaaa gaacaacgga ttcataaaag
     1801 tgctaatatc gcacattatt ttatataaca aatttatgaa aatttatggt attcccgtag
     1861 agcccgggag cagacaaaat gaaagttggt ctgacatatg acctgcagtc tgaataccta
     1921 tctttggggt actctgaaga agacactgca gagctagaca aaacagagac gattgagggc
     1981 atcgaaacag cccttcattc tttaggttat gaaaccgaac gcatcggaaa tgcaaggtcg
     2041 ctgatgatgc gtctattcaa aggagacaga tgggatcttg tttttaacat ctgcgaaggt
     2101 atcttcggag atggcaggga atcgcttgtg ccggctatcc tggatgactg gcagataccc
     2161 tacgtgtttt cgagcgctgc aactatggcc ctgtcattga acaaggcgtt atgcaaaaga
     2221 gtcgtgagag atgcgggaat acctactccc gatttttgcc tggttcgcag tatta
//
LOCUS       DAOSOF010000207         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_48641_length_2232_cov_4.031236, whole genome shotgun sequence.
ACCESSION   DAOSOF010000207 DAOSOF010000000
VERSION     DAOSOF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48641_length_2232_cov_4.031236"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2091)
                     /locus_tag="PLM68_08675"
     CDS             complement(<1..2091)
                     /locus_tag="PLM68_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HOT55467.1"
                     /translation="MKGIIDILLERTKDCPDRKIFNFLDFSTEERTVTEVTIDMVFRN
                     AKAIAWKLLDRGAKKGDRVVILSLQDPGTLYAVYGAIMAGTIFTIIPPPIDEGKVERF
                     ISVVKSCQPKFIISNYALEHERLSKAQPPQLTQPPQLQQPPQPPQSNGRSPQSGLKKQ
                     LLRQAFFEAIRLKRIYTDRINTTTRGFPLHTAAPEDVIYLQYTSGSTSDPKGVMVTLK
                     NLMTNINQVQELYDYSTGNSLAMWVPFFHNLGLLFGIFLPITALEGRTYFLQTLQFLQ
                     KPTLWLRVLSDYQVKLTAGPNSAYALFPNILTPKAASAFDLTHVTHFMNGSEFVDPVT
                     IRSFADLFGIRAEAFACGYGMAENVCLVTAAHKNHQTLWVSEDALRRNRFVPCEPGQG
                     KELVSLGEPVRGITMIAVDPATGKPCEEDQVGEMYIQGDTVCKGYWGGVRDNENFRAR
                     IEGHQGYFFKTGDMGILYGGNFYMTDRIKEIIIINGHNVYPGDIKAALKKNIPALSAD
                     ASVFFTVEANNREAVVACLETDRINLDFAALAAQVNQVAARIFEFSFYDVVFLKKNTL
                     PRTDNLKIRTHKVRELYLQEKLSQGVHKPLNERNGELPPEGMPQVLFSSRKNASAREK
                     TTSPETALNGIHEKVKSVFDNLLERQDYDLDTGFLELGGDSLKMVECACQLEEVFHIN
                     LDVTQVGLNASVNGI"
BASE COUNT          476 a          577 c          569 g          610 t
ORIGIN      
        1 aatcccgttc acagaagcat tcaggcctac ctgggtgaca tccagattaa tatgaaacac
       61 ttcttccagc tggcaggcac attccaccat cttgagtgaa tcgcctccca gctccaggaa
      121 acccgtgtcc aggtcgtaat cctgtctttc cagcaagttg tcgaacacgg acttgacttt
      181 ttcatgaata ccgttcagtg cagtttcagg gcttgttgtt ttttcacggg ccgaggcatt
      241 ttttctgctg ctgaacaaca cctggggcat tccctccggc ggaagctcgc cgttgcgttc
      301 attaagtggc ttgtggacac cctggctcag tttttcctgc agatacagct ccctgacctt
      361 atgggtgcgg atcttcaggt tgtcggtcct gggaagggta ttcttcttca ggaacaccac
      421 gtcgtaaaac gagaattcaa atatccgggc agcaacctgg ttgacctggg cagccaaagc
      481 agcaaaatcc agattgatcc tgtcagtttc caggcatgcc actacagctt ccctgttatt
      541 tgcttccacg gtaaaaaaga cggaagcgtc tgcggataag gcgggaatgt tctttttgag
      601 tgcggccttt atgtctcccg ggtacacatt atgcccgttg atgatgatga tttccttgat
      661 cctgtcggtc atatagaaat tccccccata gaggataccc atatcgcccg tcttgaaaaa
      721 atagccttga tgaccctcta tacgggcgcg gaaattctca ttgtccctga cgcctcccca
      781 atagcctttg cagacagtat ccccctggat gtacatctcc cctacctggt cttcttcaca
      841 aggttttccc gttgcggggt ccaccgcgat catggttatg cccctaacag gttcccccag
      901 gctgaccagt tcctttccct gacccggttc acacgggacg aagcggttcc ttcgcaaggc
      961 atcttccgat acccacaagg tctggtgatt cttgtgcgct gccgtcacca ggcagacatt
     1021 ctctgccatg ccgtagccac aggcgaaagc ctcggcccgg atcccgaaca aatccgcaaa
     1081 ggaacgtatg gtgaccggat cgacaaactc cgatccgttc atgaagtggg tgacgtgcgt
     1141 gaggtcaaat gccgaggctg ccttaggggt cagaatgttc gggaacaagg cgtatgcgct
     1201 gttgggccct gcggtgagct tcacctggta atcggaaagg accctgagcc agagcgtggg
     1261 cttttgcagg aattggaggg tttgcagaaa ataggtcctc ccctccaggg ccgttatggg
     1321 caggaagatc ccgaaaagca atcccaggtt atggaaaaac gggacccaca tggccaggct
     1381 gttcccggtg gaatagtcat acagttcctg cacctggttt atgttggtca tcaggttttt
     1441 cagggtgacc atcacccctt tggggtcact ggtcgagccc gaggtatatt gcaggtaaat
     1501 gacatcttcg ggtgcggctg tatgcagtgg aaacccccgt gtagtggtgt ttatgcggtc
     1561 agtataaatc ctttttaaac ggattgcttc gaaaaaagcc tgcctcagga gttgtttctt
     1621 cagacctgat tgcggcgacc tcccattgga ctgtggcggt tgtggcggct gttgcaactg
     1681 tggtggctgt gtcagctgtg gcggctgcgc ttttgaaagc cgctcatgtt ccagggcgta
     1741 attggaaatg atgaattttg gctggcagga tttgactacc gatatgaaac gctctacttt
     1801 tccctcgtct atcgggggcg ggatgatggt aaaaatggtg cctgccataa tggctccgta
     1861 tacggcatat agggtaccgg gatcctgtaa agagagaatg actacacggt ctcccttctt
     1921 tgctcccctg tccagcaatt tccaggcaat ggctttggcg tttctgaaaa ccatgtctat
     1981 ggtaacctct gttacagttc tttcttctgt agaaaaatcc aggaaattga atatttttct
     2041 gtccggacag tctttggtcc tttccaggag tatgtcaata atgcctttca tgcttctttg
     2101 actggggatt cgtctgttat gactggggat tcgcctgtca attccttcat gtgttttttc
     2161 atcccggcga agagctgcag tatcttttcc cggtcctttt cattaccccg taccgctgtt
     2221 gtgaaatcca ac
//
LOCUS       DAOSOF010000208         2065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_54129_length_2065_cov_3.667662, whole genome shotgun sequence.
ACCESSION   DAOSOF010000208 DAOSOF010000000
VERSION     DAOSOF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54129_length_2065_cov_3.667662"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..551
                     /locus_tag="PLM68_08680"
     CDS             93..551
                     /locus_tag="PLM68_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOT55468.1"
                     /translation="MNTLPLILAYIGVALMLIPSGVGSAIGTVLAGNATVAAMKKNPN
                     IFGSSMILSALPSTQGLYGFAAFFLNLGAINGLNGVLTMNQALAVLAAGTAMGVVGLI
                     SAIKQAQIAANGVVEMSNGHNVFSKTLVLAVFPELYAILAFASCFLAMPS"
     gene            555..1955
                     /gene="glmM"
                     /locus_tag="PLM68_08685"
     CDS             555..1955
                     /gene="glmM"
                     /locus_tag="PLM68_08685"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT55469.1"
                     /translation="MSLIQSISGIRGTIGGLPGVSLSPFDIVRFTSAYARLIGEQRRG
                     MSQTIVVGRDARLSGDMVRQLVCGTLRACGMDVVDIGLASTPTTEMAVLFLKASGGIV
                     LTASHNPGEWNALKLLDHNGEFLNANQGERMLEIIQKNNFEFSGVDDLGAYETNDFTD
                     KHIEAVLNHPLVNVEAIYRSGFSAVVDGINSVGGVIMPRLLDAMGVTCHKIHCEPTGH
                     FAHNPEPLPHHLLDLSSEVVARKATLGISVDPDVDRLALVCEDGSMFGEEYTLVACAD
                     HVLGKFATQYPDKKIHTVSNLSSTRALKDVTLWFGGTYSAAAVGEVNVVEEMKRTGSV
                     IGGEGNGGVILPSLHYGRDALVGTALFLSLLAEREQSMTQLRSTYPAYSMSKNRADMK
                     EGLDMDVLCDRMRKAFPGATISMVDGLRLDFENERKWVSLRKSNTEPILRIYSEAPDK
                     SGADDLASQILALLSL"
BASE COUNT          548 a          482 c          524 g          511 t
ORIGIN      
        1 aaaaatgcaa gtttcaccgg aggcggacgc gaatacaaac cgttgaaaat taaagaataa
       61 ttaatacaat atttaatatc agttaactta ttatgaacac attaccatta attttagctt
      121 acataggtgt tgcacttatg ttgatcccct ccggagtcgg aagtgctata ggaacagtat
      181 tggcaggaaa tgccaccgta gcagccatga aaaagaatcc taacattttc ggatcttcaa
      241 tgatactgag cgccctgcct tcgacccagg gtctttacgg tttcgctgct ttcttcctga
      301 accttggagc cattaatggt ttgaacggag tactcactat gaaccaggca ttggccgttt
      361 tggcagctgg cacggccatg ggtgttgtgg gtcttatttc agccatcaaa caagctcaga
      421 tcgcagccaa cggggttgtt gaaatgagca acgggcataa cgtattcagt aaaacattgg
      481 tattggccgt gttcccggaa ctctatgcca tcctggcctt tgcctcgtgc ttcctagcaa
      541 tgcccagcta aaccatgagc ctgatacaat ccatctcggg aataaggggt accattggcg
      601 gtttgccggg ggtatccctt tctccttttg acattgtccg gtttacctcg gcctatgcca
      661 ggctcattgg tgagcaaagg agaggaatgt cccaaaccat tgttgtgggc cgcgatgcac
      721 gcctgtccgg agacatggtc agacaactgg tttgcgggac gttgcgagct tgcgggatgg
      781 acgtagtgga tataggactg gccagtaccc ccacgactga aatggcggtt ctttttttaa
      841 aagcgtccgg gggaatcgtt ctcacagcca gtcacaaccc aggggagtgg aatgcgctca
      901 aactactgga tcacaacgga gagttcctca acgcaaacca gggggaaagg atgctggaaa
      961 ttatccagaa aaacaacttc gaattttccg gagtggatga cctgggtgca tatgaaacaa
     1021 atgattttac cgacaaacac atagaagccg ttcttaacca cccgctggtg aatgtggaag
     1081 caatttatcg ttcaggcttc agtgctgtcg ttgatggcat caactcggtc ggtggggtga
     1141 tcatgccaag actcctggat gccatggggg ttacctgcca caagatccac tgtgagccaa
     1201 caggccattt tgcacacaat ccggaacccc tgccccatca cctgctggac ctgtcatcag
     1261 aagttgttgc cagaaaagca acgctgggca tatctgtaga ccccgatgtg gaccggctgg
     1321 cccttgtttg tgaagacggg agcatgtttg gcgaggaata cacccttgtg gcctgtgcag
     1381 atcacgtgct gggtaaattt gccacacagt atccggataa aaaaatacat acggtatcca
     1441 atctttcctc cacccgcgcc ctaaaagatg taacgctgtg gtttggaggc acttattctg
     1501 ccgctgcggt aggagaagta aatgtggtgg aagaaatgaa acgtaccggt agcgtgattg
     1561 gcggtgaagg caatggcggc gttatcctgc cctcactcca ttatggcagg gacgcactcg
     1621 ttgggactgc cctcttcctg tcactgctgg ctgaacgtga gcaatccatg acacagctca
     1681 gaagtaccta tccggcttat tcaatgagta aaaaccgggc tgacatgaaa gaagggttgg
     1741 acatggatgt gctctgtgac aggatgcgta aagcatttcc cggtgcgaca ataagcatgg
     1801 tagacggact gcggcttgat tttgaaaacg aaagaaaatg ggtcagcctt cgcaagtcca
     1861 atacggaacc aatcctgcgg atatatagtg aggctcctga taaatccggc gcagatgatt
     1921 tggcttctca aatccttgct ttgctttcat tataatttgg atgagccaaa aaattgcgta
     1981 actttgcagc ccgtttgcag aaaattataa aattatacat aacatgaaac aaggaataca
     2041 cccggaaacc tacaggcttg tcgct
//
LOCUS       DAOSOF010000209         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_54901_length_2043_cov_4.900905, whole genome shotgun sequence.
ACCESSION   DAOSOF010000209 DAOSOF010000000
VERSION     DAOSOF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54901_length_2043_cov_4.900905"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLM68_08690"
     CDS             <1..429
                     /locus_tag="PLM68_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT55470.1"
                     /translation="FEQEYERIPSPEELSEILDIPSEKISDTLRVSGRHVSVDAPFID
                     GEDNSLLDVLVNNDSPNADRGLVNESLNKEIERALSTLSRRERDIVKYFFGIGTPDMT
                     LEEIGEEFGLTRERVRQIKEKAIRRLRHSARSKLLKAYLG"
     gene            433..1773
                     /locus_tag="PLM68_08695"
     CDS             433..1773
                     /locus_tag="PLM68_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOT55471.1"
                     /translation="MFETIGNAIHTFSSWLNLPLFITLIGGGLFFMFYSGFIPLRFYG
                     RGLKAVGVKEEGAAGQISSFQALSSAIAATVGLGNISGVAVAITIGGPGTIFWMWVSA
                     CLGMATKFFEGTLAIMFKGRDSRGELQGGTMYMITEGLGNKWKPLAVLFSIFGMVGTF
                     CLMQSNQLTEALQTVIFRPAGIENTPWLRLVVGLCIAGIVSLVVVGGINRIGKVATLI
                     VPFMVGFYFLLVFYIILTNLSVVPDVFRSIFQEAFNLKAGLGGLAGSAIVIGARRAAF
                     VNEAGVGTATMMHGASKNSEPVREGLVAMIAPSIDSGLVCTLTALAVLINAQYTVSNV
                     QGMQMVIESFERSIPGWGGYLLMIVVLAFSFSTMFSYSYYGTKCTNFLFGAHRAHYYN
                     YLFLGTLVMGAVIPLTAVVDIIDIAYAFMAFCTVFTMIRLSPNVKAAIKVYFKK"
     gene            1770..>2043
                     /locus_tag="PLM68_08700"
     CDS             1770..>2043
                     /locus_tag="PLM68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT55472.1"
                     /translation="MNAEDRIIKALENTVIKLYGITPKQSIQLQETRKEFEGDFTFVV
                     YPLLPVSRKSPEQTATELGQAIIEAVQEVESFQVIKGFLNLKMSPYY"
BASE COUNT          518 a          452 c          507 g          566 t
ORIGIN      
        1 tttgaacagg aatacgaacg catcccttct cccgaagaat tgtcagaaat actggatatc
       61 ccgagtgaaa aaatctctga caccctgcgt gtctcgggcc gccatgtatc tgtagacgct
      121 ccgttcattg acggggagga caacagcctg ctggatgtcc tggtaaacaa cgattcaccc
      181 aacgccgacc gcgggcttgt caatgaaagc ctgaacaagg agattgaaag ggcactttcc
      241 actttatcta ggcgtgaaag ggatatcgtt aagtatttct ttggaatcgg tacacccgac
      301 atgaccctgg aagagatagg agaagaattc gggcttacaa gggaacgtgt ccgtcaaata
      361 aaggaaaaag caatacgccg gcttcgtcac agtgccagaa gcaaattact gaaggcttat
      421 ttgggttaac ctatgtttga aacaataggc aacgccatcc atacattcag ttcatggctg
      481 aacctgccct tgttcataac cctcattggc ggcggtcttt tttttatgtt ctattcgggt
      541 ttcatcccgt tgcgttttta tggcagggga ctgaaggccg ttggtgttaa ggaggagggt
      601 gccgccggac aaatcagttc ttttcaggca ttgagttctg ccattgcagc caccgtagga
      661 ctgggtaaca tttccggggt ggctgtcgcc attaccatag gaggccctgg taccatattc
      721 tggatgtggg taagtgcctg cctgggaatg gcgactaaat tctttgaagg aacccttgcc
      781 atcatgttca aaggaaggga cagcagggga gaattgcagg gaggaaccat gtacatgatc
      841 acggagggac tgggcaataa atggaaaccg cttgccgtgc tgttttccat atttggtatg
      901 gtcggcactt tttgcctgat gcaatccaat caactgacag aagccctgca aacggtgatc
      961 tttagacctg ccgggataga gaatactccc tggctcaggc tggtggtagg attgtgtatc
     1021 gctggcatag tatccctggt tgttgttggg ggaattaacc gtatcgggaa agtggcgaca
     1081 ctcattgtcc cttttatggt gggcttttac ttcctgcttg ttttttacat catactcact
     1141 aatctgagtg ttgtccctga tgttttccgt tcaatattcc aggaagcgtt caaccttaaa
     1201 gcaggcttgg gaggtttggc cggttcggcc atagtgatag gtgccagaag ggcagccttt
     1261 gtgaatgaag cgggagttgg aaccgccacc atgatgcacg gggcttccaa aaattctgaa
     1321 cctgtacgtg aaggattggt ggcaatgatc gcaccctcca ttgattccgg gctcgtttgc
     1381 accctgacag cccttgcggt gcttatcaat gctcaatata cagtgtccaa tgtgcaaggg
     1441 atgcaaatgg tcattgaatc atttgaacgt tccatcccgg ggtggggggg ttacctgctc
     1501 atgattgtcg ttctggcttt ttccttttcc accatgtttt cctattcgta ttacggaacc
     1561 aagtgtacga attttttatt tggtgcccac agggcacatt attacaatta cctttttctg
     1621 ggtacccttg tcatgggagc cgtgatcccg ctgacagccg tcgttgatat aatagatatt
     1681 gcttacgcat tcatggcttt ttgcactgtg ttcactatga tcaggctctc gcctaacgtt
     1741 aaagctgcta ttaaagtata tttcaagaaa tgaatgcaga agacaggata atcaaggcct
     1801 tggaaaacac tgtaataaaa ctttacggaa taactcccaa acagtcaata cagttacagg
     1861 aaacaaggaa agaatttgaa ggcgacttta cctttgttgt ttatccattg ctgcctgtaa
     1921 gcagaaaatc tcccgaacaa acagccaccg aactcggaca ggctataatt gaagctgttc
     1981 aggaagtcga atctttccag gtcataaaag gttttcttaa tctcaagatg tcaccgtatt
     2041 act
//
LOCUS       DAOSOF010000210         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_55851_length_2019_cov_5.306517, whole genome shotgun sequence.
ACCESSION   DAOSOF010000210 DAOSOF010000000
VERSION     DAOSOF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55851_length_2019_cov_5.306517"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLM68_08705"
     CDS             complement(<1..127)
                     /locus_tag="PLM68_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55473.1"
                     /translation="MFDLDSWQEVWVTITRNKRRSLLTAFGIFWGIFMLVIMLGAG"
     gene            complement(130..1386)
                     /locus_tag="PLM68_08710"
     CDS             complement(130..1386)
                     /locus_tag="PLM68_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55474.1"
                     /translation="MFDDFQEIFTTLKKNKLRTFLTGFSMAWGIFMLIVLLGAGNGIR
                     NGILFNFADMATNFVKLWPGRTAQAYGGLQAGRRIKLDSTDGPYLEKEFSQVYNVSPL
                     ISRWGRNFYYQNEYAPGVLQGVLPGYSNIESVEIRPGNGRFINQADIRLARKVIVLHS
                     RTAGLLFKDKDPLGKHITYDNVSYKVVGVYHDTNMSQNPNSCIPLTTAQLIYNPENGY
                     SQVAMLLDGMDTKALNEVFEKDLRSSMGRKHRFEKEDQSAIYLYNVTREFIQFRGIFN
                     GITLFVWIIGLGTLIAGVVGISNIMLITVKERTKEFGIRKAIGATPGTILRLVLTECL
                     MITAFFGYLGMLAGIAVTELLNHVVGTPDLPASQEDMVLFANPTVDPVIIIAATLVLI
                     AAGLMAGYLPARRAVQVKPIEALRYE"
     gene            complement(1379..>2019)
                     /locus_tag="PLM68_08715"
     CDS             complement(1379..>2019)
                     /locus_tag="PLM68_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT55475.1"
                     /translation="HVLKGLNLQIDDGEFVAIMGASGSGKSTLLNIIGILDDYDTGSY
                     HLNGKLIRDLSEKESAVYRNEMIGFVFQSFNLISFKTALENVALPLYYKGIRRSKRNR
                     IAMEYLERMGLGEWADHMPSELSGGQKQRISIARALVANPRVILADEPTGALDSQTSV
                     EVMQLLQQVNREGMTIVMVTHNPEMAAMSQKIIKIRDGIIGSVEQNNKASHV"
BASE COUNT          488 a          521 c          467 g          543 t
ORIGIN      
        1 taccggcccc cagcatgatc acaagcatga aaatacccca gaagatgcca aaagcagtaa
       61 gcagactcct tctcttatta cgggtgatgg tgacccaaac ttcctgccaa ctatccaaat
      121 cgaacatgat tactcgtatc ttaaagcttc tatgggtttg acctgtacgg ccctgcgggc
      181 gggaaggtat ccggccataa gaccggccgc tatcagcacc agggtggctg ctattatgat
      241 gaccgggtcc accgtgggat tggcaaacag caccatgtct tcctgggaag ccggtaaatc
      301 gggggttcct accacatggt tcagcaattc tgtgacagca atacccgcaa gcattcccag
      361 gtaaccgaaa aatgccgtta tcatgaggca ttcggtaagc acaaggcgca agatagtccc
      421 gggggtggca ccaattgctt ttcgtattcc aaactctttg gttctttcct taaccgtgat
      481 caacatgatg ttactgatgc caaccacccc ggctatcaag gttcctagtc cgatgatcca
      541 gacaaacaag gtgataccgt tgaatattcc cctgaactgg ataaattcgc gggtcacatt
      601 ataaagataa atcgcagact ggtcttcttt ctcaaaccgg tgcttgcggc ccatggacga
      661 cctgaggtcc ttttcaaaaa cctcgttcaa cgcttttgta tccatcccgt ccagtaacat
      721 ggctacctgg gaatagccgt tttctggatt atagattagt tgcgcagtag tcaaagggat
      781 gcaactgttt gggttttgcg acatgttggt gtcatgatat accccaacta ccttatatga
      841 aacattgtca taggtaatat gttttcccag ggggtcttta tccttgaaaa gcaacccggc
      901 cgtcctggaa tgcagcacaa tgacttttcg tgccaggcgg atatcggcct ggttgataaa
      961 acggccgttg ccgggccgta tctcaaccga ttcaatattg ctgtacccgg gaagtacccc
     1021 ctgcaataca ccgggggcat attcgttctg gtagtaaaaa ttcctgcccc aacgcgaaat
     1081 taatggagag acattgtaaa cctggctgaa ttccttttcc aggtaggggc cgtctgtgct
     1141 gtccagcttt atccgccttc cggcctgcaa cccgccataa gcctgggccg ttcttcccgg
     1201 ccagagtttc acaaagttgg ttgccatatc ggcaaaattg aaaagaatgc cgtttctgat
     1261 cccgttcccc gcacccagca ggactatgag catgaatatg ccccatgcca tggagaagcc
     1321 ggtcaggaaa gtgcgcaact tgtttttttt cagcgtggtg aatatttcct gaaagtcatc
     1381 aaacatggct tgccttatta ttttgttcta cagatccaat gataccgtca cggattttta
     1441 ttattttctg gctcatggca gccatctcgg gattatgggt gaccatcacg atggtcatcc
     1501 cctccctgtt gacttgttgc aatagctgca tgacctcaac cgaggtttgt gaatccaggg
     1561 ctcctgtagg ttcgtctgcc agtatgaccc gggggtttgc caccagtgcg cgggcgattg
     1621 atatacgctg tttttgtcct cccgacaatt ctgaaggcat atgatccgcc cattccccca
     1681 gtcccatgcg ctccaggtat tccatggcga tcctgttgcg cttgctgcgt ctgattcctt
     1741 tgtaatataa gggaagggcc acgttctcaa gggcagtctt gaaagagatc aggttgaagg
     1801 attgaaaaac aaatcctatc atttcgttcc tgtatacggc agactccttt tccgaaagat
     1861 cccggatcag tttgccgttc aggtgataag atcctgtatc atagtcgtcc agtattccaa
     1921 taatgttgag cagggtggat tttccggacc cggatgctcc cattatcgcc acaaattccc
     1981 catcatcaat ttgaaggttc aatcctttca aaacgtgca
//
LOCUS       DAOSOF010000211         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_56424_length_2003_cov_3.879877, whole genome shotgun sequence.
ACCESSION   DAOSOF010000211 DAOSOF010000000
VERSION     DAOSOF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56424_length_2003_cov_3.879877"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLM68_08720"
     CDS             <1..786
                     /locus_tag="PLM68_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55476.1"
                     /translation="SNARFSFYPLTSGIHLSGRFLFRSRTPGWYVQQYYSNHYAWNNA
                     FDKTSDTRIEAQLSIPDWRTEATFSYGLLGKMIYYDTLGVVRQAEQPVNVMTATLTQN
                     FRLWYFRFDHRLLFQFSSNKEILPLPLFSANIRYYIEIPVVKDVMTAQIGADITFHTA
                     YFMEAYNPALGNFHVQNEKKYGNTPYIDAFIHMKWKRATIYAKYINVAQGWPDSGYFS
                     APHYIRPQRVFKLGITWPFHIMPGKTATRSTSSPGGAPVNSTH"
     gene            786..1766
                     /locus_tag="PLM68_08725"
     CDS             786..1766
                     /locus_tag="PLM68_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT55477.1"
                     /translation="MSKKKIFIALAASFLISLLIKDYKYWFSHRSEDSRTERNTGLPG
                     RDSLRSTIYLPSHAGGRVGYHYEIMKRFAGSHDKELKYIPVVDSSSVWDMLKEQHTDI
                     LAINIIHDPVPKSLAGKVCITPQITPLGEVWIMMKDHCPWHLSLVHWLTLFKQNPGFS
                     QFRNRFLPSSPDRCPYDSLLRVVSRPLGWDWKMLRSVMYQESKYRMNARSGSDAIGLM
                     QIRESTAKDMKVLNPYDPEDNIKGAVRYFAFLRRNMHLSHLPEEEEINFVLAAYNAGM
                     ARIQDDREQAAQDGLDPDKWAHVARYAPQQTQEYVEIVWNRYMGWVSASE"
     gene            complement(1750..>2003)
                     /locus_tag="PLM68_08730"
     CDS             complement(1750..>2003)
                     /locus_tag="PLM68_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55478.1"
                     /translation="AVVAIRGMIAGTVLAVVFLIIQETTRFITLDPASYFVDSVPVDI
                     SWFHILTVDVIAVGMLLLILMLPLKSLIRISPDKAIRMR"
BASE COUNT          550 a          506 c          462 g          485 t
ORIGIN      
        1 tctaatgccc gcttctcctt ttatcccctg acttcgggca tacatttgtc cggcaggttc
       61 ctgttccgtt cccggacacc cggatggtac gtgcagcaat actactcaaa ccattatgcc
      121 tggaataacg cgtttgacaa aacatctgac accaggatcg aagcgcaatt atcaatacct
      181 gactggagga cggaagcgac cttttcatac ggcctgctcg ggaaaatgat ctactacgat
      241 acactgggcg ttgtaaggca ggcagaacaa ccggtcaatg tgatgactgc aacattgacc
      301 cagaatttca ggttatggta cttccggttt gaccaccggt tgttatttca attctcgtcc
      361 aacaaggaaa tcctgccttt gcctcttttt agtgcgaaca ttaggtacta tattgaaata
      421 ccggtggtca aggatgtaat gacagcgcag ataggagccg atataacgtt ccatacggct
      481 tacttcatgg aagcatataa tccggcgctg ggtaactttc atgttcaaaa cgagaaaaaa
      541 tacgggaaca ctccttacat agatgctttt atacatatga aatggaagcg ggccaccatt
      601 tatgccaaat acatcaatgt ggcccaggga tggcccgaca gcggttattt ttcagccccg
      661 cattacatac gcccgcaacg tgtctttaaa cttggaatta cctggccatt ccatatcatg
      721 cctggaaaaa ccgccacccg gtcaacctct tccccgggag gtgcccctgt taattcaacc
      781 cattgatgtc taagaaaaaa atattcattg cccttgcagc atcctttttg atctctctcc
      841 tgataaagga ctacaaatac tggttttcgc accgttcaga agattccagg acagaaagga
      901 acacaggctt gccgggcagg gacagcctcc gtagtaccat atacctgcct tcacatgcag
      961 gaggacgcgt gggatatcat tacgagatca tgaaacgttt tgccgggtcc catgataagg
     1021 aactgaaata tataccggtt gttgactcct cctctgtctg ggatatgctc aaagagcaac
     1081 ataccgatat tctggccatc aacatcatac atgatccggt tccaaaatct cttgccggca
     1141 aggtgtgcat cacaccacag atcactcccc taggagaagt ttggatcatg atgaaggacc
     1201 actgcccctg gcacctgagc ctggttcact ggctcacatt gttcaagcag aacccgggtt
     1261 tttcacaatt caggaaccgt ttccttcctt catcccccga caggtgtcct tacgattccc
     1321 tgctgcgggt agttagcaga ccgctgggat gggactggaa aatgctcagg tctgtgatgt
     1381 accaggaatc aaaatacagg atgaatgccc gttcagggag cgatgccata ggactcatgc
     1441 agatccggga atctaccgca aaagacatga aggtccttaa tccttacgat ccggaagaca
     1501 acatcaaggg agccgtcagg tactttgctt tcctgcgaag gaatatgcat ttgtctcatc
     1561 ttccggaaga agaagagatc aattttgtcc ttgctgcata caatgcaggc atggcacgta
     1621 ttcaggacga cagggagcag gcagcacagg atggactgga cccggacaaa tgggcacatg
     1681 ttgcacggta tgcaccacag caaacacagg aatacgttga aattgtctgg aacaggtata
     1741 tgggttgggt cagcgcatcc gaatagcttt atcgggactt atcctgatga gactcttcaa
     1801 aggcagcata agtatgagca ataacatgcc aaccgctatc acatccacag taagaatgtg
     1861 gaaccacgaa atgtccacgg gtacactgtc gacaaaataa ctggccggat ccagtgtgat
     1921 gaaccgggtt gtttcctgga ttatcagaaa taccacggcc agtacggttc ccgctatcat
     1981 gccacggatt gccacaacag ccg
//
LOCUS       DAOSOF010000212         1981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_57273_length_1981_cov_5.356698, whole genome shotgun sequence.
ACCESSION   DAOSOF010000212 DAOSOF010000000
VERSION     DAOSOF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57273_length_1981_cov_5.356698"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..940)
                     /locus_tag="PLM68_08735"
     CDS             complement(176..940)
                     /locus_tag="PLM68_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT55479.1"
                     /translation="MKILIGGDIEGIATACDWDSITPGHPDYERNRLQYTHEMNAAIE
                     GAFAAGATEVYVRDGHGGNKSLIPDLLDKRAFHIKGRCSEDYRAMVLTIETGYDAVLF
                     LGYHAKAGTPDGVLCHTMARAVDEFTLNGFSLPEMGYNALVAGMYNVPVVYLSGDEAA
                     CRQAKELFGGIVTTSVKKGLSRCSAICLHPQVACDQIRADVEKALTSGKRCVYRMEGP
                     YTMYCSIRRTYDAPNIEKTLHTASLEEALHLFWTLT"
     gene            complement(972..>1981)
                     /locus_tag="PLM68_08740"
     CDS             complement(972..>1981)
                     /locus_tag="PLM68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOT55480.1"
                     /translation="IGKICLTENVNDYYDELMEMSSRTLANKLIEGLPARIDSLTSFL
                     NDEYYALLPLYNFYFTRDASAVVGNNAVICRMANKIRVRESLIMEAVFNNNQYFTSNV
                     VNTFEQQPHDRSILIEGGDILVIRDDILVVGNGMRTTSQGVDLLLNRLSGSAPEGKSY
                     IFVQQLPSSPESFIHLDMVFTMLDVDKCVVFDPLIQGDTRYSTVQITLDNGKVEKIRR
                     VPGLLTSLRRLGVDLEPISCGGSDEWDQEREQWHSGANFFAFAPGKVLSYARNEHTLN
                     EMSKHGFEIITSEDVTSGKVDINTVKKCVVTIVGAELARGGGGTRCMTLPLRRKSVKW
                     E"
BASE COUNT          455 a          525 c          490 g          511 t
ORIGIN      
        1 aagttccttt ttcccttctg gggcaacgtt cctgaaatct tcaaagaggc cggtgagaat
       61 gcctttcctg ccgagcatgc gtaccctgaa agcttccacc tcggcctggg tttttgcctg
      121 gaaaacatcc agctcttttg tcaaattatc tattatgtcc ctcataatct gcttgtcatg
      181 tcaatgtcca gaaaaggtgc agggcttctt ccagagaagc tgtatgcagt gttttctcaa
      241 tgttgggtgc atcgtatgtc cgacggatgg aacaatacat ggtgtaaggg ccttccatgc
      301 ggtatacgca acgcttcccg ctggtcagcg ctttttccac atcggcacgg atttggtcac
      361 aggctacctg ggggtgaagg cagatggccg agcatcggga caagcctttc ttgacagatg
      421 tggtcacgat cccgccgaat aattccttag cctgccggca tgcggcttca tcacccgaga
      481 ggtagaccac gggaacgtta tacatgccgg ctaccagggc attgtatccc atctccggca
      541 gggaaaaccc gttaagcgta aattcatcta ccgcgcgggc catggtgtgg cacaacaccc
      601 cgtccggtgt accggccttg gcgtgatatc ccaggaacag cacggcatcg taacctgttt
      661 caatggtcag gaccatggca cggtagtctt ccgagcaacg gcctttaatg tgaaaggctc
      721 ttttatccag cagatcggga atcagcgact tgtttccgcc atgcccgtca cggacatata
      781 cttctgtggc gcctgcagca aaggctcctt caatggcggc gttcatttca tgcgtatact
      841 ggagacggtt tcgttcataa tcgggatggc ccggtgtgat ggaatcccag tcacacgcgg
      901 tggctatacc ttcaatatct cctccaataa gaattttcat aggtcggtag tattatgctt
      961 tttatattta attattccca tttgacggat ttccggcgca ggggaagtgt catgcagcgc
     1021 gtacctcccc cgcccctggc cagttcggcc cccactatgg tcacaacaca ttttttcact
     1081 gtatttatat ccacttttcc agaggtcaca tcttccgatg ttatgatctc gaagccgtgc
     1141 ttagacattt cgttcagggt gtgttcattg cgggcataag aaagcacctt gcccggagca
     1201 aaggcaaaga aattggcacc ggaatgccat tgttcccgtt cctggtccca ctcgtcgctg
     1261 cctccgcacg atatgggttc caggtccacg ccaaggcggc gcagggaggt cagcaagccc
     1321 ggaacacgcc gtatcttttc caccttcccg ttatccaggg tgatctgtac tgtactgtaa
     1381 cgggtgtcac cctgtatgag gggatcgaac accacgcact tgtccacatc aagcatggta
     1441 aagaccatat ccaggtggat aaagctttcc ggagaggaag gcagctgctg gacaaaaata
     1501 taactcttgc cttccggagc agatcccgac aagcggttaa ggagcaggtc cactccctgt
     1561 gacgtggtcc gcataccgtt cccgaccacc aggatatcat cccttatgac caggatatca
     1621 cctccctcta tcaggataga gcgatcgtgc ggttgttgct caaaagtatt gaccacattg
     1681 cttgtgaaat actggttgtt attgaaaaca gcttccatga tgagggactc cctaacccgt
     1741 atcttgttgg ccatacggca gataacagca ttgttcccca ccacggctga cgcatccctg
     1801 gtgaagtaga aattatacag ggggagtaag gcatagtatt catcattcaa aaaagaagta
     1861 aggctgtcta tgcgggcagg gagtccttca atgagcttgt tggccagggt ccttgaggac
     1921 atttccatca gctcgtcata gtaatcgttc acgttttctg taaggcatat cttgcctatc
     1981 a
//
LOCUS       DAOSOF010000213         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_58679_length_1948_cov_5.180666, whole genome shotgun sequence.
ACCESSION   DAOSOF010000213 DAOSOF010000000
VERSION     DAOSOF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58679_length_1948_cov_5.180666"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1948
                     /locus_tag="PLM68_08745"
                     /pseudo
     CDS             398..1948
                     /locus_tag="PLM68_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
BASE COUNT          540 a          438 c          512 g          458 t
ORIGIN      
        1 gacccggaaa actggtacgt attccggggc cggattaagg ccggataagg aaggtggatc
       61 tgaaaaatgg cgaaatataa taccctttgt ctagcagaat acaaaagtgg gtaggtggat
      121 aagaagtgag aaggacgaaa tccccattag tgaaaatgtt gcattatttt gataagaaca
      181 aagcgtggcg gtgcgggcag gtagtaaaag agacaaaaac atctgtgatt gtctacactg
      241 catacaggga aaaggaaatt gtacctaggg acaagataat tagggaagaa ttcgtaacat
      301 ctgaccgggc ttacctcaga ccagactgaa aataatttag tccaagtttg tataataaag
      361 acagaaggga aaactacgca ccagagggat gtatatgatg aagttatctg aagcaggaca
      421 aagagtcttt aacgcgcgtt acgccatgcg tgacgatcaa ggatatatta ttgaaacatt
      481 tgatcaggct gtcgaacgcc tggcgcgggc tgctgcaagt gccgagaaag aaaaccaggt
      541 tttctggcaa gaaaagtttt cctcaattat aggagatctt ctgtttattc catccacacc
      601 gatttgggcc aatatgggca aacccgatcg gccctggcag ccttccgcct gctttgttct
      661 ggccatggag gattcgctgc agtccatgta cgaaaccctc ctgtctactg cgatggtctt
      721 caaatctgga ggaggtgtag gctacaactt tagcaccatc agaccgcggg gttctctggt
      781 gagatccact aagggtaggg cctcaggcgt tgttgaattg atcaagcttt ataacgcttc
      841 atccgatatg gtcatgcaag gtggtgtccg ccggggcgca tctatgggta ttttaaatat
      901 tgaccatcct gagatcatgg actttattca tgccaagcta gatggcaggc tgacgaactt
      961 taacctgtcc gtgggcgtta catacgcttt tatggacgct cttgcccagg gtgcggactg
     1021 gcctcttgta tttaacggca aagtacacga tgtattacct gccaccgagc tgtggcagga
     1081 gatcgtgaaa tcagcccatg cctgtgggga tccgggtatt atttttctgg ataccattca
     1141 gaaaagcaac cccttacccc acaaacagtt aaactgtact aacccatgcg gcgaacaacc
     1201 gctgtccccc ggcgaatctt gactgttggg cagcgtcaac ctggcccgtt taattggccc
     1261 ggacaaccgt atcgatagag ccaagttgca agaggtggtg gctctcgccg tgcgtttttt
     1321 ggataacatc atcgatgtag cagagtaccc atttgaattg atcgcagaag ccacccgggc
     1381 caccagaaaa attggcctgg gctttactgg actggccgac gcactgatcc gggccggttt
     1441 accatacgat tcagatgaag gtaggcatta tgcgggagag atagtcgagc tgatgcagca
     1501 aacaaccaga gagacgtccc gggccctggc ggaggaaaaa ggctgctttc ccgaatggga
     1561 aaacagcatc tattaccctg atgagaaaat cagaaatgct accagcgtga ccattgcgcc
     1621 caccggttcg gtaactacaa tggccggctg cgaaggttac ggtgtagaac cgatatttgc
     1681 cgtagcctat aaaaaagcta ctaatgtagc cggtgacttt gaagtgttca gcccgttatt
     1741 cctggaagcc tgtcaaaaag ccggcgttcc caacagtgtt ctggaagatg tagccttaag
     1801 cggcacctgt caggggatca tgggaatccc gcctgagatc gcccgtgtgt tcaaaggagc
     1861 tcatgatatc gcaccggaag atcatatcct gatgcaggct gaagtacaaa aacatgtgga
     1921 taatgctgta agcaaaacta ttaacctg
//
LOCUS       DAOSOF010000214         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_61867_length_1877_cov_3.709111, whole genome shotgun sequence.
ACCESSION   DAOSOF010000214 DAOSOF010000000
VERSION     DAOSOF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61867_length_1877_cov_3.709111"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..576)
                     /locus_tag="PLM68_08750"
     CDS             complement(31..576)
                     /locus_tag="PLM68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT55481.1"
                     /translation="MKEELIIAGFGGQGVLSMGKIIAYSGIMEDKEVTWMPSYGPEMR
                     GGTANVTVIISDKRISSPILRWCDTAIVLNQQSMDKFEPMVRKGGLLLYDPNGITRHP
                     VRKDIKVCSIEAADLSNRMEMSKAFNMVVLGAYLKMKPILERHNVIQGLKKSLPERYH
                     DLIPMNEKAIELGMKEVKEVI"
     gene            complement(579..1340)
                     /locus_tag="PLM68_08755"
     CDS             complement(579..1340)
                     /locus_tag="PLM68_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT55482.1"
                     /translation="MDIKDIIKPENFVYGKSKVLTDTVMHYCPGCSHGVVHKILAEVI
                     EEMGIQDKTIGISPVGCAVFAYKYIDIDWQQAAHGRAPALATATKRLYPDKYVVAYQG
                     DGDLASIGTAEIMHACNRGENIVVIFINNAIYGMTGGQMAPTTLLGMKTATTPYGRRA
                     DLNGYPLKITELIAQLEGTCYVTRQSVQNPGSVRKAKKAIRKAFENSALKKGTSFVEI
                     VSTCNSNWKMTPVKANKWMEENMFPFYPLGDIKDR"
     gene            complement(1352..>1877)
                     /locus_tag="PLM68_08760"
     CDS             complement(1352..>1877)
                     /locus_tag="PLM68_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOT55483.1"
                     /translation="PVRRLTNQEIAEKYGHWATLGKPASRNGNIISSLSLAASEMEAI
                     NLRLQAKYQKMRENEVRFEKTMCDNAEYLLVAFGSSARICEGTLEIARSKGIRMGLLR
                     PITLFPYPETAIREMLPQLKGIMSVELNAGQMVEDVRLAVNGAVPVEFYGRMGGSIFS
                     PEEVFEAFAKKFNL"
BASE COUNT          410 a          466 c          453 g          548 t
ORIGIN      
        1 aatgcatttt tagcaaaaaa aaaaagaggt ctaaatgacc tcttttactt ctttcattcc
       61 cagctctata gctttttcgt tcatgggtat cagatcgtga tacctttccg gcagggattt
      121 cttaagtccc tgtatgacgt tatgcctttc caggatgggt ttcatcttaa gataggcacc
      181 cagcacaacc atattgaaag ccttggacat ttccatgcgg ttggacaggt cggctgcttc
      241 tatggagcat accttgatgt ccttacgtac aggatgacgc gttatgccat tgggatcata
      301 aagcaacagg cctcctttcc tgaccatggg ttcaaacttg tccatggact gctggttcag
      361 cactatggcc gtatcacacc accgtaggat gggcgagctg atccgtttgt cactgatgat
      421 gaccgtcacg ttggccgttc caccgcgcat ttcggggccg taggatggca tccaggtaac
      481 ttccttatcc tccatgattc ctgaataggc aatgatcttg cccatagaga ggacgccttg
      541 tcctccaaaa cctgctataa tcaattcttc tttcataatt atctgtcttt tatgtctccc
      601 aggggataaa acgggaacat gttttcttcc atccatttat tggccttgac cggggtcatc
      661 ttccagttgg aattgcaggt tgaaacaatc tctacaaaag aggttccttt tttcagcgcc
      721 gagttttcaa aagctttccg gatggctttt tttgcttttc tgacactgcc cggattctgc
      781 acagactgac gtgtcacgta acaggttcct tccagttgtg ctatcagttc cgtgatcttg
      841 agggggtaac cgttcaggtc agccctcctt ccatagggag ttgtggccgt tttcattccc
      901 aggagggtgg tgggggccat ctggccgcct gtcataccat agatggcgtt gtttatgaaa
      961 atgacaacaa tattttcccc gcggttgcaa gcgtgcatga tctctgccgt gccaatggat
     1021 gccaggtcac cgtctccctg gtaggccacg acatatttgt cggggtacaa gcgcttggtg
     1081 gctgtggcca gtgccggggc acgtccgtgt gcagcctgct gccagtcgat atcgatgtat
     1141 ttataggcaa aaacagcaca acccaccggg gaaatcccta tggtcttgtc ctggataccc
     1201 atctcttcta tgacttctgc cagtattttg tgaactacgc cgtgactgca accgggacag
     1261 tagtgcatga ctgtgtctgt cagcactttg ctcttgccgt atacaaagtt ctcgggtttg
     1321 atgatatctt taatgtccat ggtaattgcg attaaaggtt gaatttttta gcaaaagctt
     1381 cgaatacttc ttcgggagag aagatggatc cgcccatcct tccgtagaat tcaacgggaa
     1441 cagctccgtt aacagccagc cgtacgtctt ctaccatctg tcccgcattc agttcaacgg
     1501 acatgatgcc cttgagctgt ggcaacatct cacggatggc cgtttcgggg tacgggaaca
     1561 aggtgatggg cctgagcaat cccatgcgga tgccttttga acgggctatc tccagggtgc
     1621 cttcacaaat acgggcagaa cttccgaatg ccaccaatag gtattctgca ttgtcgcaca
     1681 tggttttttc aaaacgcact tcgttttccc tcattttctg gtattttgcc tgcagccgca
     1741 ggttgattgc ttccatctcg gaggcggcca gggaaaggga agaaatgatg ttaccattac
     1801 ggctggcggg tttacccaag gttgcccaat ggccgtattt ttctgcaatt tcctggtttg
     1861 taaggcgtct gaccggt
//
LOCUS       DAOSOF010000215         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_64733_length_1817_cov_4.114075, whole genome shotgun sequence.
ACCESSION   DAOSOF010000215 DAOSOF010000000
VERSION     DAOSOF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64733_length_1817_cov_4.114075"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1817)
                     /locus_tag="PLM68_08765"
     CDS             complement(<1..>1817)
                     /locus_tag="PLM68_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOT55484.1"
                     /translation="DANGVYALNNVPENGTLVFSSIGYEAIEIPVNGRAEINAALAQQ
                     AVGLDQILVVAYGTTTKGSYSGSATQLRQESFKDVPVLTFEQAIVGAVPGVQISQKSG
                     QPGSLPEIRIRGFGSFNAGNEPLYVIDGVPATSGDWSSGNIYTSSMNFLNPGDIESVT
                     VLKDAAASSLYGSRASNGVVIITTKKGKAGRPVTTFKGNVGISYFAVNNYPLVSEEEG
                     EVLTREAWRNYGTDNPAQWQKYGSLDNYVDVMTEKYYPSRKSNLIYENWEDQLFRTAV
                     LQNYEVSISGGNENSKVFASIAYTDENGINRIQYMKRFTANVNAEHKMSKYLRVGGSI
                     QFSDQKQSGHQDGQAKDNVFYLWKVHLTPRWPFKYDDGSYWLEFYDGGTRVNPVPQFD
                     LQINDANQMRIMLKGWAELSITDGLKIKTLLSKDYLKMHDRFAWLYGHINFTAYGQGY
                     ASDRHRFIDRVVSSTIANYNKSFGKHNIALMAGWEAESEEFIYTRLAKIDFSNFGATE
                     SALASNFESGYTYRSNSNLLSFISSASYDFNSKYYVSGSYRRDGSSRLGPATRWGDFW
                     SVAGSWRLSNEEFIKGIKWINDLRIRGSYGTSGTLPSSY"
BASE COUNT          399 a          458 c          483 g          477 t
ORIGIN      
        1 gtaactggag ggcagggtgc cacttgttcc atatgatccc ctgatcctga ggtcgttgat
       61 ccattttata cctttgatga attcctcgtt gctcaatcgc caggagcctg caaccgacca
      121 gaagtcaccc cagcgggttg ccgggcccaa cctggaggat ccgtcacggc ggtaagaacc
      181 ggatacgtaa tacttgctgt tgaagtcata acttgcgctg ctgataaagg agagcaggtt
      241 ggaattactc ctgtaggtgt agcccgattc aaaattggaa gcaagtgccg attcggttgc
      301 cccgaagttg gaaaagtcaa tcttggcaag gcgtgtgtag atgaattcct cgctttctgc
      361 ttcccatccg gccataaggg cgatgttgtg ttttccgaac gatttgttgt agtttgcaat
      421 ggtgctgctt accaccctgt caatgaaacg gtgacggtcc gaggcatatc cctggccgta
      481 ggccgtaaaa ttaatgtgac catagagcca tgcaaacctg tcatgcatct tgaggtagtc
      541 cttgcttaaa agggttttga tcttcaggcc gtcggtgatg ctgagttctg cccatccctt
      601 gagcatgatc ctcatctggt tggcatcgtt gatctgcagg tcaaactgcg gcacgggatt
      661 cacccgggta ccaccatcgt agaattccaa ccagtaggag ccgtcatcgt atttgaaagg
      721 ccagcgcggg gtgaggtgca ccttccacag gtaaaatacg ttgtcttttg cctgtccgtc
      781 ctggtggccg ctctgcttct ggtcagagaa ctggatagaa ccgcccaccc tcaggtattt
      841 ggacatcttg tgttctgcat tcacattggc ggtgaaacgt ttcatgtatt gaatgcggtt
      901 gataccgttt tcatctgtgt aggctattga agcaaagacc ttggagtttt cattccctcc
      961 ggatatggac acttcgtagt tctgcaacac agccgtgcgg aaaagctggt cttcccagtt
     1021 ttcgtagatc aggttgcttt tacgagaggg ataatacttt tcagtcatga catccacata
     1081 gttgtccagg gaaccgtatt tctgccattg tgcaggattg tctgtcccgt agttgcgcca
     1141 ggcttccctg gtgaggactt ctccttcttc ttcagacacc aggggataat tattcacggc
     1201 aaaatacgaa attcccacat tgcccttgaa ggttgtgacc ggtctgccgg cttttccctt
     1261 tttggtagtg atgattacaa caccgttgga ggccctggac ccgtataggg aagatgcggc
     1321 ggcatccttg agtacggtga cagattcaat gtccccggga ttcaagaagt tcatggaact
     1381 cgtgtagatg tttcccgagc tccagtctcc actggtggca ggcacaccat caataacata
     1441 caagggttcg ttgcctgcat tgaaggaacc aaaaccgcgg atgcggattt cgggcaggga
     1501 acccggttgt cccgatttct gggagatctg cacccccgga acagcgccca cgatggcttg
     1561 ttcaaaggta agcacaggca catccttgaa gctctcctgc cttagctggg tggcagagcc
     1621 ggaataggac cccttggttg tggtgccata ggcaaccaca aggatctgat ccaggcccac
     1681 agcctgctgg gcgagggcag cgtttatctc ggcccggccg tttacaggga tctcaatcgc
     1741 ttcatagccg atgctggaga aaaccaatgt cccgttttcc gggacattgt tgagcgcata
     1801 aacaccgttg gcgtctg
//
LOCUS       DAOSOF010000216         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_66293_length_1786_cov_4.566724, whole genome shotgun sequence.
ACCESSION   DAOSOF010000216 DAOSOF010000000
VERSION     DAOSOF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66293_length_1786_cov_4.566724"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PLM68_08770"
     CDS             complement(<1..157)
                     /locus_tag="PLM68_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOT55485.1"
                     /translation="MRFFIDTANLEQIRQARDLGILDGVTTNPSLMAHENIHGHAAIM
                     AHYRQICE"
     gene            277..>1786
                     /locus_tag="PLM68_08775"
     CDS             277..>1786
                     /locus_tag="PLM68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT55486.1"
                     /translation="MQFTIRAIHQKCAALKLLLFITFLALAIVSCSREEPEDILSDKA
                     DLISFVLSKAENPSLSTDLQGIRNRNVFYMTVPEGVDLTNLKPSLLVSPGATVRINNV
                     PVTSSGSNPTASDSGSDPLASSGSNPTASDSGSDPLASSGSNPTASDSGSDPLASSSH
                     YYPPATRSMPAVDFSGVVTVEVTAENGKALQYKLLVKNGRQEFDNLIYQCMAKYDIPG
                     ISFAFSKNEEIAYCSGLGFAITETEERTTPGHLFRLASISKQFTTLCILKLYEAGKLT
                     LDDRVFGTGGILEQEYGNVTPRAAAVTIRHLLQHTSGWGSDPDPMFSSAFTGQTLDQR
                     IRYMLGTTQAEPGTVFTYSNMGFGILGEVIKKVSGQPYEEYLKAVLSEAGITDIHVGK
                     DRAGRRPNEVVYYSQNGYNGYANPMDVIAAAGGVIASTEQMLQLLFHIDGRPIVPDII
                     QPATRIQMLTPSAIYPRYALGWRRGHKFFPDAAYHSGNLAGTATMWVMGSSET"
BASE COUNT          444 a          504 c          468 g          370 t
ORIGIN      
        1 tttcacaaat ttgccggtaa tgcgccatga tggccgcgtg gccgtggatg ttctcgtgcg
       61 ccataagcga agggttggtg gtcaccccgt ccaggatccc caggtcacgc gcctggcgta
      121 tctgctccag attggctgtg tctatgaaaa atctcataat atttttaatt ttgtctaccc
      181 aaagatacgc acaaaaatga ctgccgcgca cttttgatgt atcccgctgg attacaacgt
      241 aataacaaaa cagctccccg gaagggctct ttttatatgc aattcactat cagagcaata
      301 catcaaaagt gcgcggcact caagctcctt cttttcataa ctttccttgc cttagccatt
      361 gtttcttgtt ccagggaaga acccgaggac attttaagcg acaaggcgga cctgatctcg
      421 ttcgtcctgt caaaggccga gaacccctcc ctgagcactg acctgcaggg aattcgcaac
      481 aggaacgtgt tttacatgac cgtgcccgag ggtgtggacc tgaccaacct gaagcccagc
      541 ctgttggtgt cgccgggcgc aacggtgagg atcaacaacg tgccagtcac aagcagtgga
      601 agcaatccta cagctagcga cagtggcagc gatcccctgg caagcagtgg aagcaaccct
      661 acggctagcg acagtggcag cgatcccctg gcaagcagtg gaagcaaccc tacggctagc
      721 gacagtggca gcgatcccct ggcaagcagt agccactact atcccccggc aaccaggagc
      781 atgcctgcag tggatttttc gggagtggtg acggtagagg ttactgcgga aaacgggaaa
      841 gccctgcagt acaagctgct ggtgaaaaat ggcaggcagg aatttgacaa cctcatctac
      901 caatgcatgg ccaagtacga tatcccgggg atttcctttg ccttctctaa aaacgaagag
      961 atagcctatt gcagcgggct gggttttgcc atcacggaaa ccgaagaaag aaccacgccc
     1021 ggccaccttt tcaggctggc aagcatctct aagcaattca ccacattatg cattctgaaa
     1081 ctgtacgagg caggaaaact gacccttgac gacagggttt tcgggaccgg aggcatcctg
     1141 gagcaggaat atggcaacgt tacacctcgt gccgcagcgg tcactatcag gcatttgctg
     1201 caacacacca gcggctgggg cagcgacccc gaccccatgt tctcctcggc cttcaccggc
     1261 cagacgcttg atcaaaggat acggtacatg ctgggcacca cccaggccga gccgggcacc
     1321 gtgtttacat atagcaatat gggtttcggg attttgggag aggtcatcaa gaaagtttcc
     1381 ggacaaccct acgaagaata cctgaaagcg gtcttatcag aggcgggcat taccgacatc
     1441 catgtaggaa aagaccgcgc gggcagaagg ccgaacgaag tggtttacta ctcccagaat
     1501 gggtacaacg gctatgcaaa tcccatggat gtgattgccg cggctggcgg tgtcatcgcc
     1561 tcgaccgaac aaatgctgca actgctcttt cacatagacg ggcgccccat cgtgcccgat
     1621 atcatacaac ccgccacgcg tatccagatg ctcacccctt ctgcgatcta tccccggtat
     1681 gccctgggct ggagaagagg ccacaaattc ttccccgacg ccgcctacca ctccgggaac
     1741 ctggctggaa ccgctaccat gtgggtgatg ggcagttccg agacag
//
LOCUS       DAOSOF010000217         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_68386_length_1747_cov_4.437352, whole genome shotgun sequence.
ACCESSION   DAOSOF010000217 DAOSOF010000000
VERSION     DAOSOF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68386_length_1747_cov_4.437352"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLM68_08780"
     CDS             <1..866
                     /locus_tag="PLM68_08780"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT55487.1"
                     /translation="YNIANIHVPKTIDNDLPLPEGIPTFGYQSAKAQGTDLGRTVYED
                     ARTSENWFIVTAMGRSAGHLAFGIGSSCHFPMIIISEMFYKTQMTIDKIVNLIVSSII
                     KRQIRNIPYGCIMISEGVFQSLSEEDVKNAGIAFTYDAHGHPELGKISKSHIIDNLVE
                     KKMKELGINVKTRPVEVGYEVRCITPKSFDLEYCSMLGMGVYELYTKGVSGCMVCRDG
                     NGKIIPLFLQDLQDPATGKIPPRVVNMNSHEVQFYMKHIMDYITEEDYEAAKAIVPNP
                     EEFDFHKILKF"
     gene            complement(962..>1747)
                     /locus_tag="PLM68_08785"
     CDS             complement(962..>1747)
                     /locus_tag="PLM68_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOT55488.1"
                     /translation="FNYEGGCYAKCIDLSQEKEPDIWKAIRRDALLENVTILPDGTPD
                     YADGSVTENTRVSYPIYHINKIVLPSKAGHAKKVIYLSADAYGVLPPVSILDEKQAQY
                     HFLCGYTSKLAGTERGITEPLPSFSAAFGEAFLTVHPTIYAKNLVKKMKENGAKAYLV
                     NTGWNGTGKRISLKDTRAIIDAILDGSIDKASTRHIPVMNLTIPVKLPKVSEGILDPR
                     DTYTDVKEWEKKALSLAAKYIKNFEQYCDNDAARQLVASGPQV"
BASE COUNT          436 a          404 c          448 g          459 t
ORIGIN      
        1 agtacaacat agccaacata cacgttccca aaaccataga taacgacctt ccccttccgg
       61 aaggaattcc cacgttcgga tatcagagtg ccaaggcaca gggcaccgac cttggacgga
      121 ccgtatacga agatgcccgc accagcgaaa actggtttat cgtaacggct atgggacgtt
      181 cggcaggcca cctggctttt gggatcggtt cttcctgtca ttttcccatg atcatcattt
      241 ccgagatgtt ctacaaaacc cagatgacca tagacaagat agtgaacctg atcgtttctt
      301 cgatcatcaa acgccagatc cgtaacattc cttacggttg tatcatgatc tcggaaggcg
      361 ttttccagtc cctttcggaa gaagacgtaa agaatgcggg cattgcgttt acttacgatg
      421 cacacggcca tccggaactg ggtaaaatat ccaaatccca tatcatagac aacctggtgg
      481 agaaaaaaat gaaagaactg ggtatcaatg ttaagacacg gcctgtggaa gtgggttatg
      541 aagtccgttg catcactcca aaatcctttg acctggagta ttgttccatg ctgggcatgg
      601 gcgtatatga attgtatacc aaaggtgtaa gtgggtgcat ggtatgccgg gacgggaatg
      661 gcaagatcat tccgttattc ctgcaggacc tgcaggatcc ggctaccgga aagatccctc
      721 cgcgcgttgt aaatatgaat tcacacgagg tccagttcta catgaagcat atcatggatt
      781 atattacaga agaggactac gaggccgcca aagccatcgt tccgaatccc gaggaatttg
      841 actttcacaa gatcttgaaa ttttaagggt tcattcagtc gctgcacaac aaagaaaaga
      901 gagctttttt ggtacgccgg ggctctcttt tctttgttgt actgcgttat ggtacgcggc
      961 actatacctg gggacctgaa gccaccagct ggcgtgcagc atcgttgtcg caatactgtt
     1021 caaagttttt gatgtacttt gctgcaagag aaagcgcttt cttctcccat tcctttacgt
     1081 ctgtgtaagt gtcgcgcgga tccaggatac cttccgaaac cttgggcaat ttgacaggaa
     1141 tggtgagatt catgacgggt atgtgccttg tggaggcttt gtcaatgctt ccgtccagga
     1201 tggcgtcgat aatggcacga gtatctttca gggaaatacg tttgccggta ccgttccacc
     1261 ctgtgttcac caggtaggct ttggccccgt tttctttcat ctttttgacc aggtttttgg
     1321 cataaatggt cgggtgcacg gtcaggaaag cctcgccgaa ggctgccgag aatgagggta
     1381 atggctcggt gatgccgcgt tcggttcctg caagttttga agtgtaaccg cacaggaagt
     1441 gatactgtgc ctgtttctca tccaggattg aaaccggggg taaaacgccg taggcatcgg
     1501 cagaaagata aatgactttt ttagcgtgac cggccttgga aggaaggacg atcttgttga
     1561 tatggtatat gggataggaa acacgcgtgt tctccgttac agacccgtct gcataatcgg
     1621 gggtgccgtc cggcaaaatg gttacgttct ccagcagggc atcacgacgg atggctttcc
     1681 agatatcggg ttctttttcc tggctgaggt caatgcattt ggcatagcag ccaccttcat
     1741 aattgaa
//
LOCUS       DAOSOF010000218         1669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_72878_length_1669_cov_4.360595, whole genome shotgun sequence.
ACCESSION   DAOSOF010000218 DAOSOF010000000
VERSION     DAOSOF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72878_length_1669_cov_4.360595"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..690)
                     /locus_tag="PLM68_08790"
     CDS             complement(4..690)
                     /locus_tag="PLM68_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOT55489.1"
                     /translation="MLTFLKEKVFQANLMLVEHGLVIFTWGNVSAIDHETGLVVIKPS
                     GVSYRTMTAGQMVVVDKDGKVVEGDLRPSSDTPTHLALYRAFEGIGGIVHTHSVYATS
                     WAQAGVDLPNIGTTHADYFHGAIPCTAAMNKKQINRNYEWETGNMIIERFRDMDPMHT
                     PAVLVKNHGPFTWGADPFQAVENAVVLEQVAKMAAVAYSINPSLEMNQLLIDKHFERK
                     HGPDAYYGQK"
     gene            complement(700..>1669)
                     /locus_tag="PLM68_08795"
     CDS             complement(700..>1669)
                     /locus_tag="PLM68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HOT55490.1"
                     /translation="DPDFPWLGALLGSSIIGFWYWCTDQYIVQRVLSGRNQKESRRGA
                     IFGAYLKLTPVFLFLIPGMIAYAMSQKGVTINGQLFVLNTADTAFPQLVATLLPAGVK
                     GMVVCGILAALMSSLASLFNSSAMLFTIDFYKRFKPQTPEKKLVRIGQIATVVIVILG
                     ILWIPVMRSIGDVLYAYLQDVQSVLAPGIAAAFLMGIIWKRASAKGGMWGLITGFVIG
                     LTRLGAKVYYTSVETATQSGIFYRVFYETNWLFFCGWMFLFCVIVIILVSLFTKAPQP
                     AMIQGLVFGTATAAEKAETRASWNHWDVIHTLIILGITSAFYWYFW"
BASE COUNT          452 a          418 c          392 g          407 t
ORIGIN      
        1 gaactatttt tgtccgtaat aggcatccgg tccgtgtttg cgttcaaagt gcttgtcaat
       61 cagcaattga ttcatttcca gtgaaggatt aatggaataa gccacagctg ccattttggc
      121 cacctgttcc aggacaacgg cgttctccac cgcttggaac ggatctgctc cccaggtgaa
      181 tggtccgtga ttcttcacaa gcactgcagg agtatgcatg ggatccatat ccctgaaacg
      241 ctctatgatc atatttcccg tctcccattc ataattcctg ttgatctgtt ttttgttcat
      301 ggcagcggta cagggaatgg caccatgaaa ataatctgca tgggttgtac cgatattggg
      361 caggtcaaca cctgcctgtg cccatgaagt agcatagacg gaatgggtat ggacaatgcc
      421 cccgatacct tcaaaagcac ggtataaggc aagatgcgtg ggggtatccg aggatggcct
      481 cagatctcct tccacgactt ttccatcctt gtcaacaacc accatctggc cggcggtcat
      541 ggtacggtaa cttactcccg agggtttgat gaccacaagg cccgtttcat ggtctatggc
      601 cgaaacattt ccccaggtaa aaatcaccaa accatgttcc accaacatga gattggcctg
      661 gaatactttt tcttttaaaa atgttaacat gttgctaaat taccaaaaat accaatagaa
      721 cgctgatgtg atgcccagga tgatcaatgt gtggataaca tcccagtgat tccagctggc
      781 tcttgtctcg gctttctctg cagctgtggc tgtcccgaat accaggccct gtatcatggc
      841 cggctgtgga gctttggtga ataggctcac cagtatgata acaataacac agaagaggaa
      901 catccagccg cagaagaaca accagttcgt ttcatagaaa acacgataga aaatgcccga
      961 ttgagttgct gtttcaacgc tggtataata cactttggcc cctagccggg taagtccgat
     1021 cacaaatcca gttatcagtc cccacatgcc ccccttggca ctggcacgtt tccagatgat
     1081 ccccataagg aatgcggcgg caatacccgg ggcaagaaca gattgaacgt cctgcaaata
     1141 ggcgtagaga acatccccaa tgcttctcat gaccggtatc cataaaatgc ccaggattac
     1201 gatcacaacg gtagcgatct gacctatgcg gaccagtttc ttttcaggtg tctgcggctt
     1261 gaaacgttta tagaaatcta tggtgaacag catggcagag gaattgaaca acgatgccag
     1321 ggaactcatg agcgcagcca ggatgccgca aaccaccatt cccttgacac ctgcaggcag
     1381 caaggtggct accaattgcg gaaaagcggt atctgctgtg ttgagtacaa aaagctgtcc
     1441 gttaatggtc acgccttttt gtgacatggc atatgctatc ataccgggaa taaggaaaag
     1501 gaatacgggc gtcaatttga ggtatgcacc aaaaattgcc cccctgcgcg attctttctg
     1561 gttccggcca gaaaggacac gttgtactat atactgatct gtacaccaat accaaaaccc
     1621 gatgatggat gaacccagca aggcacccag ccaggggaaa tcgggatcg
//
LOCUS       DAOSOF010000219         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_75984_length_1620_cov_4.235144, whole genome shotgun sequence.
ACCESSION   DAOSOF010000219 DAOSOF010000000
VERSION     DAOSOF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75984_length_1620_cov_4.235144"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..658
                     /gene="coaE"
                     /locus_tag="PLM68_08800"
     CDS             47..658
                     /gene="coaE"
                     /locus_tag="PLM68_08800"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT55491.1"
                     /translation="MKTPIILGITGGIGSGKSYVTRIFSALGVPSYDSDARTKQLYRE
                     DPALRELLIGILGPDVFSNGEPDTRKIASIIFNNTDLLKQINRTVHPYVVRDFNKWAA
                     ACHAPYVIFESAILLEMDPPFRATRVLTVSADRELRLHRTAGRDNVRIQSVMARMDKQ
                     WTDEQREAKADFVVLSDDKQALLPRVLEVHQYMLNLTNNLNEN"
     gene            648..1187
                     /locus_tag="PLM68_08805"
     CDS             648..1187
                     /locus_tag="PLM68_08805"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOT55492.1"
                     /translation="MKTDLSKILSVGGQPGLHRYLTQSSGGVVVESLSDGKRKWMGPS
                     ARMTSLSDISVYTQTDEELKLQDVFVSMKEYLKDEQAPAPGKDPAEMAAFFEKVIPQY
                     DRNRFYPSHMKKVLEWYNLLKSADALDFEEAEGKEEAAAAAEPVAKKPAAKTIIPAAK
                     AAKPVAAKAPKPVQHTTQK"
     gene            1187..>1620
                     /locus_tag="PLM68_08810"
     CDS             1187..>1620
                     /locus_tag="PLM68_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT55493.1"
                     /translation="MQTSRRIPQRVSVYTLGCSKNRVDSEHLMRQLQAGGMKVIPDAL
                     EEDYPLDTLIINTCGFIHDAREESVNYILEALLAKEEGRLGKVLVMGCLAERYKKDLE
                     KEMPGVDAFFGVTDIPLITAYLGVPYHPRLATERVLSTPAHYA"
BASE COUNT          458 a          409 c          405 g          348 t
ORIGIN      
        1 tgaaaaagaa ttatatagca gtaatgctac tattaaaaat ttacgtatga aaactcccat
       61 catactaggc atcacaggcg gcataggcag tggaaaaagc tacgtgaccc gtattttcag
      121 cgcattggga gttccttcat acgattcaga tgcccgtacc aaacaacttt acagggaaga
      181 tccggctctt cgtgaactgc tcatagggat cctcggaccg gatgttttca gcaacgggga
      241 acccgacacc aggaaaattg cttccatcat attcaataac acggacttgc taaaacagat
      301 caaccgtacg gtccatcctt atgttgtacg ggattttaat aagtgggctg cagcctgcca
      361 tgccccctac gtaatttttg aaagtgccat cttgctggaa atggatccac ccttccgggc
      421 cacccgggta ctcaccgtct cggcagaccg ggaactcagg ctccaccgca cagcaggccg
      481 cgacaatgta cgaatccagt ctgttatggc cagaatggat aaacaatgga cagatgagca
      541 gagagaagcc aaggcagatt ttgttgtcct gtcagatgat aaacaggcac ttttgccccg
      601 ggttcttgaa gtacaccaat atatgttgaa tcttacgaat aacctcaatg aaaactgatc
      661 tttcaaaaat tttgtcagta gggggtcaac ccggtttgca ccgttacctg acccagagca
      721 gcgggggtgt ggtagttgaa tccctctcgg atggaaaaag aaaatggatg gggcccagcg
      781 cccgcatgac atcgttgtca gatatttctg tatacacaca gacagatgag gagctgaagc
      841 tccaggatgt atttgtcagt atgaaagaat atctgaaaga cgaacaggct cctgcgccgg
      901 gaaaggaccc ggccgaaatg gctgcctttt ttgaaaaagt cattccacag tacgacagga
      961 accgttttta cccctcgcac atgaaaaaag tgctggaatg gtataacctg ctcaaatcgg
     1021 cagacgcact ggactttgag gaagcagaag gaaaagaaga agcagcggca gcagcggaac
     1081 ctgttgccaa aaaacctgct gccaaaacga tcattcctgc tgccaaggca gcaaaaccgg
     1141 ttgcagccaa ggctccgaag ccggttcagc acacaaccca gaaatagtgc aaaccagccg
     1201 acggatcccc caaagggtat cggtctatac cctgggctgt tccaagaacc gtgtggacag
     1261 cgaacacctc atgcgtcagc tgcaggcagg aggcatgaag gtcattcccg atgccctgga
     1321 agaagactac ccgctggata cgttgataat caacacttgc ggcttcattc acgatgccag
     1381 ggaggagtcc gtcaattata tcctggaagc cttgctggcc aaagaagagg gcaggctggg
     1441 aaaagtcctg gtcatgggct gcctggctga acgctacaaa aaagacctgg aaaaggagat
     1501 gccgggtgtg gatgctttct ttggcgtaac ggacattccc ctgattaccg cataccttgg
     1561 agttccttac catccccgcc tggccaccga aagggtgctt tccaccccgg ctcactatgc
//
LOCUS       DAOSOF010000220         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_77601_length_1595_cov_6.364935, whole genome shotgun sequence.
ACCESSION   DAOSOF010000220 DAOSOF010000000
VERSION     DAOSOF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77601_length_1595_cov_6.364935"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLM68_08815"
     CDS             <1..1185
                     /locus_tag="PLM68_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT55494.1"
                     /translation="AFGIFWGIFMLVIMLGAGNGLAEGMASDTQGFSENSALFYTNNT
                     TEPYKGFQKGRYWNMEEEDLLILQAQISDIKYTVPFLMGSNVVVSYRDKSTSCNTKGM
                     SQRYNDMLPQHILYGRYINYMDVQERRKVCVVGKKVYEEIFHSGEDPTGQYIKLYNIN
                     FQIIGVTEPAVRGVNINGRDDEVIAIPYTVMQQIQNSGTVVHLIGVVANENVGMSVLE
                     PQVETLLKARHNVSPTDEFALESINVEKIFRQFEMLFTGIALLIWIVGLGTLLAGVVG
                     VSNIIMVTVRERTKEIGIRRAIGAKPVAIIFQIIKESTLLTVVAGFLGLAFGVFILDL
                     ADKLFIQNMTGDVFFKNPQIDFSVALLAAAIIVLCGVIAGILPARRALQIMAIDAIRE
                     EN"
     gene            1204..>1595
                     /locus_tag="PLM68_08820"
     CDS             1204..>1595
                     /locus_tag="PLM68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOT55495.1"
                     /translation="MKKIFRYVLIAALLAGVVFTFVYLWKKSRPKKEVYELVTPVIGN
                     IEQKTVATGKVEPRDEVAIVPQISGIVSELHKEAGEMVQVGEIIARIKVIPEMGTLNA
                     AESRVTQAGLNLNQAQRLFDRTQELFKAG"
BASE COUNT          449 a          330 c          421 g          395 t
ORIGIN      
        1 gcttttggca tcttctgggg tattttcatg cttgtgatca tgctgggggc cggtaatggc
       61 cttgccgagg ggatggcttc cgatacgcag ggtttttctg aaaactcagc tttgttttat
      121 accaataata cgactgaacc ctataaagga tttcaaaaag gtcgttactg gaacatggaa
      181 gaagaggacc tgctgatcct gcaagcacag attagtgaca taaagtatac ggtcccgttt
      241 ttaatgggca gcaatgttgt ggtatcatac agggacaaga gtaccagttg caataccaag
      301 gggatgtcac aacggtacaa cgacatgctg cctcaacata tcctttacgg aaggtatatc
      361 aattacatgg acgttcagga aaggaggaag gtgtgtgtcg ttggcaaaaa agtgtacgaa
      421 gagatctttc acagtggaga agatccgaca ggacaataca tcaaattata caatatcaat
      481 tttcagataa ttggtgtcac ggaacccgcg gtacgcgggg tgaatatcaa cggccgggat
      541 gacgaggtca tagccattcc ctatactgtg atgcaacaga tccagaactc gggaacggta
      601 gtgcacctca taggggttgt ggcaaatgaa aatgtgggca tgagcgttct ggaaccccag
      661 gtggagacct tgttgaaagc ccggcataac gtatctccca ccgatgaatt tgccctggaa
      721 agcatcaacg tggaaaaaat attcagacaa tttgaaatgc tgttcacagg aatagccctc
      781 ctgatctgga ttgtaggact gggaaccttg cttgcgggtg ttgtgggtgt gagtaacatc
      841 atcatggtca ccgtaaggga gcgtaccaag gaaattggca ttcgcagggc aatcggtgca
      901 aaacctgtcg ccatcatatt ccagatcatc aaggaaagca ccttgcttac cgttgtcgcg
      961 ggtttcctgg gactggcatt tggtgttttt atccttgacc tggcggacaa gctgtttata
     1021 cagaacatga ccggtgatgt cttctttaag aatcctcaga tagatttttc cgtggccctg
     1081 ctggctgcag ccataatcgt gctgtgcggt gtcatagccg ggatccttcc tgcccggagg
     1141 gcattacaga ttatggccat agatgcaata agagaagaaa actaacaaat aaattaccct
     1201 ataatgaaga aaatttttcg ctatgtgttg attgcagccc tgttggcggg tgttgtgttc
     1261 actttcgttt acctgtggaa aaaatcacgc cccaagaaag aggtatacga actggtcacc
     1321 cctgtaatcg gaaatattga acagaaaaca gtggctacgg ggaaagtgga accacgtgac
     1381 gaagtggcta ttgtcccgca gatctcaggc attgtgtccg agctgcataa agaagcagga
     1441 gaaatggtgc aagtgggaga gatcattgcc cgtatcaagg tcattcctga aatgggaacg
     1501 ttaaatgctg cagaatcaag ggtcacgcaa gcaggattga acctgaacca ggcacagcgg
     1561 ctttttgacc gcacccagga attattcaag gcagg
//
LOCUS       DAOSOF010000221         1532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_82159_length_1532_cov_3.570752, whole genome shotgun sequence.
ACCESSION   DAOSOF010000221 DAOSOF010000000
VERSION     DAOSOF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82159_length_1532_cov_3.570752"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="hutI"
                     /locus_tag="PLM68_08825"
     CDS             <1..957
                     /gene="hutI"
                     /locus_tag="PLM68_08825"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOT55496.1"
                     /translation="RGLSYKEIAARGGGILNSARLLRETSEEELYQQSLARAKELICF
                     GTGAVEIKSGYGLTVEDELKMLRVIRRIAQETPLTVRATFLGAHAVPEKYKGNQSGYV
                     DLIIEEMIPRVAEEKLARYVDVFCETGFFTVKETGRIFTAALRHGLRPRIHANQMDFS
                     GGVQVAVDYGAISADHLEHTGPKEWDTLAGSDTMPVLLPGATFFLEMEYARAREMIDR
                     GLPVALATNYNPGSCPAGNMQFMMTLACLKMKMTPEEAINAATLNGAHAMDLSYSLGS
                     ITVGKKANIFITAPMPSYEYFIYAFTSPLVETIILNGKITKT"
     gene            954..>1532
                     /locus_tag="PLM68_08830"
     CDS             954..>1532
                     /locus_tag="PLM68_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT55497.1"
                     /translation="MIFTLAQQFSTLLGLLTIVPTGHGAVCLKLEDKIIYIDPYSQTT
                     DYTGYPKADIIFFTHDHYDHYDTTALKHLVTAETVFVGPPCMEKHLTLEKTMKNGDSY
                     RWENIEILAVPAYNILNKRPDGQPFHPKGYGNGYVFTFGDIKVYISGDTELIPEMDNL
                     GPIDVALLAKNLPYTMSDDQFIQAVKKIGAPMV"
BASE COUNT          422 a          379 c          383 g          348 t
ORIGIN      
        1 cgcggattgt cttataagga aatagcggca cgcggaggcg gcattctcaa ttccgcccgc
       61 ctgttgcgtg aaacatcaga agaagagctt taccagcaaa gcctcgcaag ggcaaaggag
      121 ttgatttgtt ttggcaccgg cgccgttgaa attaaaagcg gctatggcct taccgtagag
      181 gacgaattga aaatgctgcg tgtcatcaga aggatagcgc aggagacgcc cctgacggtc
      241 agggcaacct ttctgggtgc gcatgcggtt ccggaaaagt acaaggggaa ccagtccgga
      301 tatgtagacc tgatcataga agaaatgatc cccagggtag ctgaagaaaa actggcccgg
      361 tatgtggatg tgttttgtga aacaggcttc tttacagtaa aggaaacggg aaggatcttc
      421 acggcagccc tcaggcacgg attgcggccc aggatccacg ccaaccagat ggatttttcc
      481 ggcggggtgc aggtagccgt ggattacggg gccatatcgg cagatcacct ggaacacaca
      541 ggaccgaagg aatgggacac tttagccggg tctgacacca tgcctgtgct ccttcccggg
      601 gccacctttt tcctggaaat ggaatacgcc cgtgccaggg aaatgataga ccgcgggctt
      661 cccgttgccc tggcgaccaa ctataatcca gggtcctgtc cggccggaaa catgcagttt
      721 atgatgaccc tggcctgcct gaaaatgaaa atgacccccg aggaagccat caatgcagcc
      781 accctgaacg gggcgcatgc catggatctg tcgtattcgc tcggatccat aacggtgggc
      841 aaaaaggcca atatatttat cacagccccc atgccctcgt atgaatattt tatctatgcc
      901 tttaccagtc ctttggtgga gacaatcatt ttaaacggaa aaattacaaa gacatgattt
      961 ttacattagc acaacaattt tcaactttat tgggtttgct taccattgtc cccacgggcc
     1021 acggagccgt atgcttaaaa ctggaagaca agatcattta tatcgatccg tattcccaga
     1081 ccaccgatta caccggttat cccaaggcag atatcatttt ctttacccat gaccattatg
     1141 accattacga cactacggcc ctgaagcacc tggtaaccgc agaaaccgtg ttcgtgggcc
     1201 cgccctgcat ggaaaaacac ctgacattgg aaaaaaccat gaaaaacggg gattcctacc
     1261 ggtgggaaaa cattgaaatt ctggctgttc cggcctataa tatcttgaat aaaaggcccg
     1321 atggccagcc attccacccc aaaggatacg ggaacggtta tgtgtttact ttcggggata
     1381 ttaaagtata tatttccgga gacacagaat taatcccgga aatggataac ctgggaccca
     1441 tagatgtagc cctgttagct aaaaatttac cctataccat gagtgacgat cagttcattc
     1501 aggctgtcaa aaagattgga gcccccatgg tg
//
LOCUS       DAOSOF010000222         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2352
            NODE_83932_length_1509_cov_5.925034, whole genome shotgun sequence.
ACCESSION   DAOSOF010000222 DAOSOF010000000
VERSION     DAOSOF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864419
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:58:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,768
            CDSs (total)                      :: 1,734
            Genes (coding)                    :: 1,715
            CDSs (with protein)               :: 1,715
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 10 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 4 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83932_length_1509_cov_5.925034"
                     /isolate="AMR_MDS_2352"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLM68_08835"
     CDS             complement(<1..908)
                     /locus_tag="PLM68_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT55498.1"
                     /translation="MKKILNILVLGAVCLVAASCATTRIPADFSGIVCGEPVFDVQEN
                     GTVKISFCVEVPANYFEKRITFSIMPSILYANGDVKELPCYTVQGFSVVDTNYPVVDW
                     SVQQVLCYSTTIPYHDGLATATLQTKAWILNCLSKEELCLPLCSWAISVPLTPVPPVY
                     MAHIAAESSVRAALRGKIYFPVNGHLVTDAIATQPEITRTLNSLRQLTSRNDFNITRI
                     DVEGNASPEGTSRINDPLAKRRADHTRAFFEKALKDNGYTKPVPASVWTVNSTSGMGF
                     WNEFYTAMKESNVSNKDQLAEKFLRLA"
     gene            1061..>1509
                     /locus_tag="PLM68_08840"
     CDS             1061..>1509
                     /locus_tag="PLM68_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOT55499.1"
                     /translation="MHIAIAGNIGSGKTTLTRLLAKHYGWVPKYEEVVDNPYLTDFYE
                     DMKRWSFNLQIFFLNRRLAGIVEIQKAGHTVIQDRTIYEDASIFATNLHAMGLMSTRD
                     FDNYIKLYELMISLIPAPDLIIYLRASVPTLVNRIQKRGREYENSIRL"
BASE COUNT          391 a          363 c          372 g          383 t
ORIGIN      
        1 gccaggcgga ggaatttctc ggcaagctgg tctttgttgc ttacgttgga ttccttcatg
       61 gcggtgtaga attcgttcca gaatcccatg cccgaggtac tgttcacggt ccatacgctg
      121 gcaggcactg gtttggtgta accattgtct ttcaatgctt tctcgaagaa agcgcgggta
      181 tggtcggcac gtctcttggc cagcgggtcg ttaatgcggg aggtcccttc cggagaagcg
      241 ttaccttcaa cgtcaatgcg tgtgatgttg aaatcgttgc gacttgtcag ctgacgcagg
      301 gaattcaacg tccgtgtgat ctctggctgg gtggctatgg catcggtaac aagatgaccg
      361 tttaccggga agtagatctt tccgcgcagg gcggctctta cactcgattc agcggctatg
      421 tgggccatat atacgggagg cacgggggtc agcggcacgg aaatggccca gctgcaaagg
      481 ggcaggcata attcttcttt gctcaggcag ttgagtatcc atgctttggt ttgcagtgtg
      541 gccgtggcca gaccgtcatg atacggtatg gtggtgctgt agcacaatac ctgttggaca
      601 gaccagtcca ccaccgggta attggtgtct acaacagaga atccctgtac cgtatagcag
      661 ggcaattcct taacatcacc gtttgcgtat aatatggaag gcatgatgct gaaggtgatc
      721 cttttttcaa aatagtttgc aggaacttcc acgcagaaag agatctttac cgtcccgttt
      781 tcctgaacgt caaaaaccgg ctctccacaa acaattccgg aaaaatcggc aggaatcctc
      841 gtggttgcgc aggatgccgc cacaagacac accgctccga ggacaagaat attcaaaatt
      901 ttcttcatat tatttaaggt tatgaatagt agaaatctat cgggttatac gcaaagttat
      961 gtaaaaaatt cccacgcaac aacaattttt gttaattacc tgttaattgt tttcaaggca
     1021 taaaatttac gtatctttgt tacagcaaaa aacaccggac atgcatatag ctattgcagg
     1081 aaacattgga agcggaaaaa ccaccctgac acgacttctt gccaaacatt acgggtgggt
     1141 gcccaagtat gaagaagtgg tagacaaccc ctatctaaca gatttctacg aagatatgaa
     1201 acgctggtct ttcaatctgc agatattctt cctgaaccgg cgcctggcag gcattgtcga
     1261 gatccagaaa gctggacaca cagtcatcca ggaccgtacc atttatgagg atgccagcat
     1321 ctttgccacc aacctgcacg ccatggggct catgtccacg cgtgattttg acaattacat
     1381 caaactctat gagctgatga tatcactcat acccgcaccc gacctgatca tatacctgag
     1441 ggcttcggtc cccaccctgg tgaaccgcat ccagaaaagg ggccgggagt atgaaaattc
     1501 catacgcct
//
